LOCUS       DATROY010000001        64484 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.001, whole genome shotgun sequence.
ACCESSION   DATROY010000001 DATROY010000000
VERSION     DATROY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64484
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1074
                     /locus_tag="VBD11_000001"
     CDS             <1..1074
                     /locus_tag="VBD11_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6952986.1"
                     /translation="EATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYELFKQ
                     LWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEKLSD
                     NLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINTMEY
                     IMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQPDL
                     EQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQKMP
                     FRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYKAKL
                     TYCLNKGASERELQEIDSLIYDN"
     gene            1199..3184
                     /gene="tkt"
                     /locus_tag="VBD11_000002"
     CDS             1199..3184
                     /gene="tkt"
                     /locus_tag="VBD11_000002"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6952987.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            3396..3701
                     /locus_tag="VBD11_000003"
     CDS             3396..3701
                     /locus_tag="VBD11_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6952988.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            3721..4455
                     /locus_tag="VBD11_000004"
     CDS             3721..4455
                     /locus_tag="VBD11_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6952989.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            4459..6063
                     /locus_tag="VBD11_000005"
     CDS             4459..6063
                     /locus_tag="VBD11_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6952990.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPISYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(6271..7657)
                     /locus_tag="VBD11_000006"
                     /pseudo
     CDS             complement(6271..7657)
                     /locus_tag="VBD11_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            7798..8601
                     /gene="proB"
                     /locus_tag="VBD11_000007"
     CDS             7798..8601
                     /gene="proB"
                     /locus_tag="VBD11_000007"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6952991.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            8611..9864
                     /locus_tag="VBD11_000008"
     CDS             8611..9864
                     /locus_tag="VBD11_000008"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6952992.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGTKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            9947..10894
                     /gene="rsmH"
                     /locus_tag="VBD11_000009"
     CDS             9947..10894
                     /gene="rsmH"
                     /locus_tag="VBD11_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6952993.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            10909..11235
                     /gene="ftsL"
                     /locus_tag="VBD11_000010"
     CDS             10909..11235
                     /gene="ftsL"
                     /locus_tag="VBD11_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6952994.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            11239..13497
                     /locus_tag="VBD11_000011"
     CDS             11239..13497
                     /locus_tag="VBD11_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6952995.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            13499..14509
                     /locus_tag="VBD11_000012"
     CDS             13499..14509
                     /locus_tag="VBD11_000012"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6952996.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            14607..15950
                     /locus_tag="VBD11_000013"
     CDS             14607..15950
                     /locus_tag="VBD11_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6952997.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            16088..16900
                     /locus_tag="VBD11_000014"
     CDS             16088..16900
                     /locus_tag="VBD11_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6952998.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            16995..17798
                     /locus_tag="VBD11_000015"
     CDS             16995..17798
                     /locus_tag="VBD11_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6952999.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            17798..18541
                     /locus_tag="VBD11_000016"
     CDS             17798..18541
                     /locus_tag="VBD11_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6953000.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            18663..18887
                     /locus_tag="VBD11_000017"
     CDS             18663..18887
                     /locus_tag="VBD11_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6953001.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            18956..19870
                     /gene="trxB"
                     /locus_tag="VBD11_000018"
     CDS             18956..19870
                     /gene="trxB"
                     /locus_tag="VBD11_000018"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6953002.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            20028..21488
                     /locus_tag="VBD11_000019"
     CDS             20028..21488
                     /locus_tag="VBD11_000019"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6953003.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            21485..22306
                     /gene="nadE"
                     /locus_tag="VBD11_000020"
     CDS             21485..22306
                     /gene="nadE"
                     /locus_tag="VBD11_000020"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6953004.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            22419..23753
                     /gene="pepC"
                     /locus_tag="VBD11_000021"
     CDS             22419..23753
                     /gene="pepC"
                     /locus_tag="VBD11_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6953005.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(23800..26040)
                     /locus_tag="VBD11_000022"
     CDS             complement(23800..26040)
                     /locus_tag="VBD11_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6953006.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(26027..26626)
                     /gene="recU"
                     /locus_tag="VBD11_000023"
     CDS             complement(26027..26626)
                     /gene="recU"
                     /locus_tag="VBD11_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6953007.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            26701..27219
                     /locus_tag="VBD11_000024"
     CDS             26701..27219
                     /locus_tag="VBD11_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6953008.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            27340..27672
                     /gene="gpsB"
                     /locus_tag="VBD11_000025"
     CDS             27340..27672
                     /gene="gpsB"
                     /locus_tag="VBD11_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6953009.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            27691..28059
                     /gene="rnpB"
                     /locus_tag="VBD11_000026"
     ncRNA           27691..28059
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBD11_000026"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            28142..29296
                     /locus_tag="VBD11_000027"
     CDS             28142..29296
                     /locus_tag="VBD11_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6953010.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            29309..30796
                     /locus_tag="VBD11_000028"
     CDS             29309..30796
                     /locus_tag="VBD11_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953011.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(31021..31503)
                     /locus_tag="VBD11_000029"
     CDS             complement(31021..31503)
                     /locus_tag="VBD11_000029"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6953012.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            31692..33299
                     /locus_tag="VBD11_000030"
     CDS             31692..33299
                     /locus_tag="VBD11_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6953013.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            33428..34324
                     /locus_tag="VBD11_000031"
     CDS             33428..34324
                     /locus_tag="VBD11_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953014.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYIEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            34311..35051
                     /locus_tag="VBD11_000032"
     CDS             34311..35051
                     /locus_tag="VBD11_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953015.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            35048..36133
                     /locus_tag="VBD11_000033"
     CDS             35048..36133
                     /locus_tag="VBD11_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953016.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            36135..36725
                     /locus_tag="VBD11_000034"
     CDS             36135..36725
                     /locus_tag="VBD11_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953017.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            36775..37479
                     /locus_tag="VBD11_000035"
     CDS             36775..37479
                     /locus_tag="VBD11_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6953018.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            37646..38275
                     /gene="gmk"
                     /locus_tag="VBD11_000036"
     CDS             37646..38275
                     /gene="gmk"
                     /locus_tag="VBD11_000036"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6953019.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            38298..38612
                     /locus_tag="VBD11_000037"
     CDS             38298..38612
                     /locus_tag="VBD11_000037"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6953020.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            38686..41076
                     /locus_tag="VBD11_000038"
     CDS             38686..41076
                     /locus_tag="VBD11_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6953021.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            41123..42058
                     /locus_tag="VBD11_000039"
     CDS             41123..42058
                     /locus_tag="VBD11_000039"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6953022.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            42048..43370
                     /gene="rsmB"
                     /locus_tag="VBD11_000040"
     CDS             42048..43370
                     /gene="rsmB"
                     /locus_tag="VBD11_000040"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6953023.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            43408..44145
                     /locus_tag="VBD11_000041"
     CDS             43408..44145
                     /locus_tag="VBD11_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6953024.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            44145..46100
                     /gene="pknB"
                     /locus_tag="VBD11_000042"
     CDS             44145..46100
                     /gene="pknB"
                     /locus_tag="VBD11_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6953025.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            46259..46954
                     /locus_tag="VBD11_000043"
     CDS             46259..46954
                     /locus_tag="VBD11_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6953026.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            46951..47970
                     /locus_tag="VBD11_000044"
     CDS             46951..47970
                     /locus_tag="VBD11_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953027.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            47963..48604
                     /locus_tag="VBD11_000045"
     CDS             47963..48604
                     /locus_tag="VBD11_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953028.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            48646..50046
                     /locus_tag="VBD11_000046"
     CDS             48646..50046
                     /locus_tag="VBD11_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6953029.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            50048..50404
                     /locus_tag="VBD11_000047"
     CDS             50048..50404
                     /locus_tag="VBD11_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6953030.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(50450..51226)
                     /locus_tag="VBD11_000048"
     CDS             complement(50450..51226)
                     /locus_tag="VBD11_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6953031.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEKIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            51347..52102
                     /locus_tag="VBD11_000049"
     CDS             51347..52102
                     /locus_tag="VBD11_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6953032.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            52221..53204
                     /locus_tag="VBD11_000050"
     CDS             52221..53204
                     /locus_tag="VBD11_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6953033.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQEDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            53408..53731
                     /locus_tag="VBD11_000051"
     CDS             53408..53731
                     /locus_tag="VBD11_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6953034.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            53748..54068
                     /locus_tag="VBD11_000052"
     CDS             53748..54068
                     /locus_tag="VBD11_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6953035.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            54070..55371
                     /locus_tag="VBD11_000053"
     CDS             54070..55371
                     /locus_tag="VBD11_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6953036.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            55552..58008
                     /locus_tag="VBD11_000054"
     CDS             55552..58008
                     /locus_tag="VBD11_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6953037.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            58018..58686
                     /locus_tag="VBD11_000055"
     CDS             58018..58686
                     /locus_tag="VBD11_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6953038.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            58754..59842
                     /locus_tag="VBD11_000056"
     CDS             58754..59842
                     /locus_tag="VBD11_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6953039.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(59994..60920)
                     /gene="cysK"
                     /locus_tag="VBD11_000057"
     CDS             complement(59994..60920)
                     /gene="cysK"
                     /locus_tag="VBD11_000057"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO6953040.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(61011..61655)
                     /locus_tag="VBD11_000058"
     CDS             complement(61011..61655)
                     /locus_tag="VBD11_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6953041.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            61711..63000
                     /locus_tag="VBD11_000059"
     CDS             61711..63000
                     /locus_tag="VBD11_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6953042.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            63000..63666
                     /locus_tag="VBD11_000060"
                     /pseudo
     CDS             63000..63666
                     /locus_tag="VBD11_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            63743..64297
                     /gene="raiA"
                     /locus_tag="VBD11_000061"
     CDS             63743..64297
                     /gene="raiA"
                     /locus_tag="VBD11_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6953043.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT        21247 a        10261 c        12846 g        20130 t
ORIGIN      
        1 gaggctactt taaatcgtca tcttgctcat ctcaatgaat tgttagcgga atttgatatt
       61 gctatttcac aaggaaaaca gattggtgat gaattgcaat ggcgttattt ttattatgaa
      121 ttattcaagc aactatggtc ttatgacaag tgccaaaata tgataaagaa attagactta
      181 gactcattaa ttctattaat agagcgcttg gctcaacata ctttaactcg agaagctcag
      241 caaaatctgg ggctttggtt tagtatttgt catcatcgtc tccttgctat ggaaaaacta
      301 tctgataatc tcaagcccat tgtggaacat tatcaatgta atgcttttta taaacggtta
      361 gacgcagcct tagttttgta tatgagtcgt tttgctcttg agtataggga aggagaagtt
      421 ctggcaactt ttgcttttct acattcacaa aatattttac caatcaatac aatggaatac
      481 atcatgggat ttggaggccc gattattgat tgtgtaaccg aaacaatcat ctactttaaa
      541 aaggagagta ttttagctga cgagacatca gatcaggtta tttaccaact tggtcagtta
      601 tatagccatt attatttctt taaaggtcat attttagttg aacaacctga tttagaacaa
      661 acgtatcgac ttattgatca taatatgagg gataagttgc atgatatttc aaagaagatt
      721 attgcaaatg ttaatcgaat tagaccttta acagaagatg gttgttcttt gctgacttta
      781 catttgttgg aattgcttat tttctcgaaa aatagtcaaa aaatgccctt taggattggc
      841 ctagatatga caggtaatgc tgtggaacag tcattgttgg aatatcgcat tcgacaacat
      901 ttctctggta ataattctat tcaagtcgaa ccttatgacg aaggaaaggg atttgatatg
      961 gttatttatc agagtcgctc aagaccttat aaagccaaac taacctattg ccttaataaa
     1021 ggtgcttcag agcgagaact acaggaaata gattcactta tttatgacaa ttaatcccat
     1081 tacttaattg aatggtttta attcctccag aaattgttaa aacgtttacg tattttatgt
     1141 tataatatca aagacgaaca attttaaatt tagttgttaa aaacagaaag gttttatcat
     1201 gacatttgac accattgatc aattagcggt taatacagtc cgcacgcttt ctattgatgc
     1261 tatccaagca gcaaattctg ggcacccagg tcttcctatg ggagctgcgc ctatggctta
     1321 tgtgctttgg aataaattct taaatgtaaa cccaaaaaca agtcgcaatt ggacaaaccg
     1381 tgaccgtttt gtactttcag ctgggcatgg ttcagctctt ctttatagcc tacttcattt
     1441 agctggctat gatttatcaa ttgatgattt aaaacaattc cgccaatggg gatcaaaaac
     1501 acctggacat ccagaagtta accatactga tggtgtagaa gcaacaactg gacctttagg
     1561 gcaaggtatt gctaatgctg ttggtatggc aatggctgaa gctcatttgg ctgctaaatt
     1621 taataaacca ggctttgatt tagttgatca ttacacttat acacttcatg gtgatggttg
     1681 tttgatggag ggtgttagcc aagaagctgc cagtcttgct ggacacttaa aacttggtaa
     1741 attggtcctt ctttatgatt caaatgatat ctcacttgat ggtccaacgt ctcaatcatt
     1801 tacagaagat gttaaagggc gttttgaatc atatggttgg caacatatct tagttaaaga
     1861 tggtaatgat ttagaagcga ttgctgcagc aattgaagct gctaaagctg aaactgataa
     1921 accaaccatt attgaagtta aaactattat tggatttggc gcagagaaac agggaacatc
     1981 ttcagttcat ggagcgccac ttggtgcaga aggtattact tttgccaaga aggcttatgg
     2041 atgggaatat ccagacttta ctgttcctgc tgaagttgca gctcgatttg cctcagatct
     2101 tcaagcgcgt ggtgcgaagg cagaagaggc atggaatgat ctttttgcta aatatgaagt
     2161 tgagtatcca gaattagcag ctgaatacaa agaagctttt gctggccaag ctgagacagt
     2221 tgaattgaaa gctcatgacc taggttcatc agtggctagt cgtgtttcaa gccaacaagc
     2281 tattcaacaa ttatcgactc aattaccgaa cctctgggga gggtcagcag acctctcagc
     2341 ttctaataac acaatggtag cagcagaaac agatttccaa gcaagtaact acgctgggcg
     2401 taacatttgg tttggtgtac gtgaatttgc aatggctgct gcaatgaatg gaattgcgct
     2461 tcatggtggg actcgtgttt atgggggtac attctttgtc ttctcaaact acttgttacc
     2521 agcagtacgt atggctgcac ttcaaaactt gccaactgtc tatgttatga cacatgattc
     2581 tattgctgtt ggagaagatg ggccaactca tgagccaata gaacaattag catcagtgcg
     2641 ctcaatgcct aatttgaacg ttatccgtcc cgcagacggt aatgaaacaa acgctgcatg
     2701 gcaacgagct gtatcagaga cagatcgacc aacgatgctt gttttaacac gtcaaaactt
     2761 accagttctt gaaggaacat cagaattagc ccaagaaggg gttaataaag gtgcttatat
     2821 tttatcagaa gctaaaggtg aacttgatgg tatcatcatt gcaacaggtt cagaagttaa
     2881 attagcttta gatactcaag acaaacttga gtcagagggc atccatgttc gcgtcgtatc
     2941 tatgccagca caaaacattt ttgatgaaca agaggcttca tatcaagagc aagtgcttcc
     3001 atcagctgtt acaaaacgtc ttgctattga ggcaggatca agcttcggtt ggggtaaata
     3061 tgttggttta aatgggctaa cacttactat tgatacttgg ggagcatcag caccaggtaa
     3121 cagaattttc gaagagtatg gctttacagt tgagaatgct gtatcgctat ataaagaact
     3181 ttaagagcat gaagaagttg ggaaaaatac ctcaacttct ttttgtaaga tataggatgg
     3241 atttgagaat agttttgata gactgataag tgatggaaag cactcttttt tgaaaacgtt
     3301 tgacaaacat tccatagcgc agtataattg ataaaactat tgtatatata aaataataca
     3361 actattatta attatctttt tagaaaggtt tgtttatgaa gaaggagtgt cgtgattttt
     3421 atcggcagat acagcatact tacaatgata tatctgtccg tgaagatgca gtgctatcat
     3481 caatactatt atcagcatcg aatggtttga taaaaactag cgatgtacct cgtgttgctt
     3541 atgagttaac tcagcaattg gaaaataatg agatagaaaa atctttcgag tcattagcta
     3601 cagttaagga attaaaaaaa tctgctaaaa aattgtctga gagatatgag caaaataagg
     3661 taacaacggg attgctttct aaagtatttg gaaatcatta ggagataaaa gaagattttt
     3721 atgattaagt ttgaacatgt ttcgaaagta tacggggaaa aagaagctct cagtgattta
     3781 accctctcca ttaaagatgg tgagattttt ggattaatcg gtcacaatgg agcaggaaaa
     3841 acaacgacaa ttagtattct aacatctatt attgatgcaa cttatggcca agtctatatt
     3901 gatgacctac ttttgacaga acatcgtgac caaattaaga aaaaaatagg ttatgtaccg
     3961 gactcgccag atattttctt gaatttaaca gcagaggaat actggtactt tttagctaaa
     4021 atttatgatg ttgccccaga agatattgaa gcacgtatca caaaattggt agacatattt
     4081 gaactagagg agcagcgcta taatcctatt gaaagttttt cacatggtat gcgtcaaaaa
     4141 gtcattgtta taggtgccct tcttccaaat cctgacattt ggattctaga tgagccatta
     4201 actggtttag atcctcaagc ttcttttgat ttgaaagaaa tgatgaaaga acatgctaaa
     4261 aatggaaaaa cagttatttt ttcaacacat gtcttagcag ttgcagaaca gttatgcgac
     4321 agaataggta ttttgaaaca agggaaatta atctttgtcg gtagcttagg tgagttaaaa
     4381 atgcaatatc cagataaaga tttggaaact atctatttgg aattagcagg acgtcaagct
     4441 aatagggagg gataaagtat gaattggtca cgtatctggg aactcgtaaa aattaatatc
     4501 ctttattcaa accctcagac tctatcggca ctaagaaaaa agcaagaaaa gcatcctaaa
     4561 aaagaatttt cagcttataa atccatgttt agaaatcagt tatttcagat tttgctcttt
     4621 tcaataattt atgtatttct ctttgtatca cttgatttta aagaatatcc gggctatttc
     4681 acgttctaca ttggtatctt tacactagta tccattatct actcttttat tgcgatgtac
     4741 agtgttttct atgagagtga cgatgttaaa caatatgctt atttgcctat taaatctgaa
     4801 gagctttatg ttgctaaaat ttttgcaaca tttgggatgt ctgtaacttt tctaatgcca
     4861 atcctaacct taatgattgt ggcttattgg cgtataatag gtggcccact tgcggtcctc
     4921 ctagcaatca taaattttgc tattttattt ttatcggtaa cggtgatttc attgtatatt
     4981 aatagtttaa taggccgtgc cattattcga agtgctaatc ggaaacttat ttctactata
     5041 ttaatttcac tagctacatt tggagcgata gttcccttat tatttgtgaa tatgaccagt
     5101 caaaagatgg tacaagggaa attacaagat atcgctccaa tttcttatgt cagaggttat
     5161 tatgatattg ttacagcgcc gtttagtatg gagagtcttc tcaactatta tctcccgctc
     5221 ttaatcattt tattcttgat aggtgctatt tataaatggg ttatgccacg ctactatcag
     5281 gagttgttgt atggacaggt caagcagaga aaagtccatc gtcaaatcga ttttagtaaa
     5341 cgtgagtcaa ttaataaaac tcttgttaaa caccatttat caagtttaca aaatgcgaca
     5401 ttattgacaa atacattttt aatgccacta ttatatctag caatgttcat tgttccaatt
     5461 ttgaataatg gtaaagagat tggtcgattt tttaatgaaa attattttgg gatagctttt
     5521 ctagcaggaa tattaatagg tagtttatgt gtgatgccag ctagcattgt aggggtaggt
     5581 atttctttag aaaaaagtaa tttctacttt ataaaaagtt tgcctatatc attttcgtat
     5641 ttcttaaagc ataaatttgt aactttgata acattacaac ttgcagttcc tacttttatt
     5701 tatttcttgg taggattctt tctacttaaa ctatcaatcc tcgttttatt gagcttcatt
     5761 ttaggtctag tcttcatggg acttatcgaa ggccagttta tttatagacg tgactataaa
     5821 catttgttct tgaattggca agaggttacg caactattta atagagggtt aggccaatgg
     5881 ctgttagttg gttcattgtt tggaatgatg ataataggta gttttcttat tggaataagt
     5941 attttttggt caatggtatg gaatactgta gctgtcaata tcataatcct tatcattggg
     6001 ttgctcatat tatcaatttg ccaatactta ctgctaaaaa atttttggaa gaagcttgtt
     6061 tagtaaaaag attttagaaa aatcatacag taagaataat gcttaataac tgagatagtt
     6121 atagcggata aaataaaggc ttgttgtgga ctttacggtt ggaaggcagc cactgtaatc
     6181 ctttgtttaa taacgctagt tttagctcta aaatatttga tttttattgc tatctattat
     6241 atcttatcag acaaagagac tagggataac ctagtctctt tgtctgatgc taacttgttt
     6301 ttcgtaatat tgtgctagaa tgaatgtcaa tatagcagtt gtgcttacag taatgataac
     6361 tgcaataagt atggtaataa aaagatgtgt tgaagtatga ctgatataaa gaaatagacc
     6421 tggtagacca gaagagccta cagcgaagcg gtgagcgttc attataccgg catagagtcc
     6481 tccaagacca ccaccaataa gtgaggctat tagaggataa cgtttcggaa ggttgacacc
     6541 atataagata ggttcggtga ttcccatata agctgttatg gcagcaggtc cagcaatttc
     6601 tttaagcttt ttttcttttg ttataagagt caccactgta cctgcagttg cttgtgcgat
     6661 atttgagcag agcatgcctg gtccaaaaat agaatcaacc cctaatttcg ctagttgtgt
     6721 gattcctaat gcagcaattc cactatgaac accaaacatg attaggatag gagcaaatgc
     6781 tcctactaga aaagcaggtg cccaactggc gtatttggat aaaaaaagta aaaataacag
     6841 ctaaatattg gccagcgata gtacccattg gtcccaatat agagaacgac aaaattccca
     6901 tgctgagaaa tattaacata ggaacaagca ctaaacgtag tgatttagga ataatttgat
     6961 ttaaatattt ttctatataa gcctgaacac aaataattag aataatggga atgacacttg
     7021 atgcatagtt agtaagtgtg aatgggatcg tatggaataa ggatactggt ttaccagaag
     7081 ctactaaatt agcccaatta gggtgaagga gcatgacaac agttcccaaa gctaaaatag
     7141 gattagcctt caatttttga gcagctgtga tggcgattaa gataggtaaa aaatagaaaa
     7201 caccatctgc aaaaagattt agtaatatat atgtttgaga taacggtgtc aagattttga
     7261 atactagtaa taaagctagc aatgccttaa tcataccagc gcctgataga gctggtaaaa
     7321 tcggttggaa agtcccagaa acaaaatcaa taattcgact aactgctttg gcttttttgg
     7381 tttgttctgt tttagtcgtt gagttagttt caatgaggga gttaatttct tcaaacactt
     7441 ttgcgacatg agtcccgatc actatctgat gttgccctcc gctgataacg acagttttta
     7501 cctcttttaa agtttttaat tgctcaaggt ttactttcgt cggatcattg agactaaagc
     7561 gaaggcgtgt ttgacaatgg taaacattgt taatattatt ttgcccacca aggtgtttga
     7621 tgatattttt tgacaaggtg atgtattctc tagacataat atctccttaa tacttataaa
     7681 acgttttccg attctagatt atagcatagt aataaaagaa aacacttgga aatgcttgtg
     7741 aattttcaat gatgttttca gatagcattc ctaaagggtc tatgatataa tttttttatg
     7801 aaaagacatt ttgagacaac tagaagaatt gttattaaag taggaaccag ttccctcgta
     7861 cagacaagtg ggaaaattaa tttaagtaaa atagaccatt tagcatttgt tatttctagt
     7921 cttatgaata aaggtatgga ggtgatttta gtatcatctg gagctatggg gtttggttta
     7981 gacattttga aaatggataa gcgtcctcaa gaaatatcac aacaacaagc agtttctagc
     8041 gtaggacaag tagccatgat gagtctttat tctcagatat tttcgcacta tcaaactcat
     8101 gtttcccaaa ttttattaac acgtgacgtt gtggtttttc cagagagttt gcaaaatgta
     8161 acaaactctt ttgagagtct gctctctatg ggaattttgc caatcgtcaa tgagaatgat
     8221 gctgtcagcg tggatgagat ggatcataaa acgaagtttg gggacaatga ccgtctctct
     8281 gctgtcgttg ctaaaattac taaagcagat ttattaatta tgctgtcaga tattgatggt
     8341 ctttttgata aaaatcctaa tatttatgat gatgctgttt tacgtagtca tgtttctgag
     8401 atcaccgatg acatcattaa gtcagctgga ggagcaggaa gtaagtttgg tactggtggc
     8461 atgcttagta aaattaaaag tgcacaaatg gtgtttgata acaatggaca aatgatattg
     8521 atgaatggcg ccaatcctcg tgatatttta aaggtgttag atgggcataa tattggaact
     8581 tattttgctc aaggaaagtg agattgagtt atgacgtata ttgaaatatt agggcaaaat
     8641 gctaaaaaag ctagtcaatc agtagcacga ttaagtacag ctagtaaaaa tgagatactc
     8701 agggacttag cgagaaacat agtggctgat actgaaacca ttttgactga gaatgctcgc
     8761 gatgttgcta aggcaaaaga taatggtatc tcagaaatta tggtggaccg tctacgacta
     8821 aacaaagacc gcatacaggc gatagcaaac ggtatttatc aggtagcaga tttggcagat
     8881 ccgattggtc aagttgttag tggttataca aatttagatg gtttaaaaat ccttaaaaaa
     8941 agagtgcctt taggggttat tgcaatgata tttgaaagtc gtcccaatgt atcggtggat
     9001 gcctttagtt tagcttttaa aacagggaac gctattattt tacgtggtgg taaagatgct
     9061 attttttcaa atactgcctt ggttaattgt atgcgccaaa cattgcaaga tacaggccac
     9121 aacccagata tagtacaatt agttgaagat actagtcatg ttgtagcaga ggagttaatg
     9181 caggcaacag actacgtgga cgtcttaatt ccaagaggtg gtacaaaatt aattcaaacg
     9241 gtgaaggaaa agtcaaagat tcctgttatc gaaacaggtg ttggaaatgt tcatatctat
     9301 attgatgaat ttgcagattt agatatggca gctaagattg ttattaatgc taaaacccaa
     9361 cgccctagtg tctgtaatgc tgctgaaggg ttagttgttc atcaagctat tgctaaggga
     9421 tttttatctc aattggagaa aatgctcaaa gaatcgaacc agtcagttga atttcgtgcc
     9481 gatgaggaag ctttacaact tttagaaaat gctgttgctg caagtgagac tgattatgct
     9541 actgaatttt tagactatat tatgtcagta aaagtagtag attcttttga acaagcaatt
     9601 agctggatta ataaatacag tagtcatcac tcagaagcta tcattactaa taatatcagt
     9661 agggcagaaa tttttcagga tatggtagat gcagcggcag tttatgttaa tgcttcaaca
     9721 cgttttacag atggtttcgt ttttggactc ggagcagaga ttggtatttc gactcaaaaa
     9781 ttacacgcgc gtggaccgat gggattagaa gctttaacaa gtacaaaata ctatattaat
     9841 ggaactggtc aagtaaggga atagttgacc tataatagga gagattgcat ttatcggtct
     9901 ctcttttact tttctaaagt ttaggattta tggtacaatt aaagttatga caaacgattt
     9961 tcatcatatt actgtgcttt tgcacgagac tgtggatatg cttgatatta agccggatgg
    10021 tacctatgta gatgctactt taggtggggc aggacacagc gaatatcttc tttcacaatt
    10081 aggcccagat gggcaccttt acgcatttga ccaagaccag aaggctattg acattgctca
    10141 tatacgttta aagaaatatg tggataccgg tcaagtaacc tttatcaaag ataatttcag
    10201 aaatctatct tctaacttaa aagcattagg agtgtcggaa attaatggta tttgttatga
    10261 tttaggtgta tcaagcccac agctggacga gcgtgaacgc ggtttctcat ataaacaaga
    10321 tgcccccctt gatatgcgaa tgaatagaga gcagagcctt acagcttatg acgttgtgaa
    10381 cacttatagt tatcatgatt tagtaaggat tttctttaag tatggcgaag ataaattttc
    10441 aaaacaaatt gctcgaaaaa ttgagcaagt acgagctgaa aaaccaatat ctacaacaac
    10501 agaattggct gagattatca aatcgtctaa atctgccaaa gaactgaaga aaaaaggtca
    10561 cccagctaaa caaatttttc aagctattcg tattgaagtc aatgacgaac taggagctgc
    10621 agatgaatct atacaacaag caatggactt attagctgtt gatggtcgta tatctgtcat
    10681 cacttttcat tctttagaag atcgtcttac taagcaactt tttaaagaag catcaacagt
    10741 agaagtacct aaaggtcttc cttttatacc agatgattta caacctaaga tggagctcgt
    10801 taatcgtaaa cccatcttac caagtcaaga agaactagaa gcgaacaatc gtgcccattc
    10861 agcgaaattg agagtagcgc gtcgtattcg ataataagaa agaaacttat gacaaatgaa
    10921 aaacgaactg aagcagtaac acaaacctta caacgtcaca tcaagacttt ctctcgcatt
    10981 gagaaagctt tttatggcgc tattgtaatt actgctatta tcatggcagt cggtattatt
    11041 tacttgcaaa gtaatagttt acaagttaaa caagaagtaa atcaattaaa tagtaaaatc
    11101 aacgataaac agacagaatt tgacaatgct aaacaagaag taaatgagct ttctaatcgt
    11161 gatcgaatta ctaaaattgc taaagatgct ggtttaacaa ttcaaaacga caatatttat
    11221 cggaaggtgg actagtctgt gacttttttt aaaaagctaa aaaaaatctt ccttgattat
    11281 gtgatccata taagagatag gcgttcgccg cagaaaaata gggaaagagt aggtcaaaat
    11341 ctgatgattt taaccatttt cctatttttc atttttatca tcaactttgt catcatcgtt
    11401 ggtacagata gtaaatttgg ggttaattta tccaaggaag ccaagaaagt ctatcaacaa
    11461 agtatgacag tacaggcgaa gcgtgggaca atttacgacc gtaatggaaa tcctatcgct
    11521 gaggatgcta cgacctatag tctctacgct attatctcaa agaactatac gacagctaca
    11581 ggtcaaaaac tatatgtaca accttctcag tatgaaaaag tagctagtat cttagagaat
    11641 aaattgggca tgaagaaaaa tcttgtccta aaacaactca atcaaaagaa attgtttcaa
    11701 gtttcctttg gaagttcagg ttcaggattg tcttatacca agatggctga catcaagaaa
    11761 acgatggaaa agtcagatat caaaggaatt ggtttttcta ccagtccggg gcgtatttat
    11821 ccaaatggga tatttgcttc acaatttata ggatttactc ttcctcagga tgatggagat
    11881 ggaaaaaaat tagttggtaa tacaggcctt gaagcggcac ttaacaaggt attatcaggt
    11941 actgatggca aagtaacata cgaaaaagac cgtagtggga atgttctttt aggtactgca
    12001 acaacagaac gtagggcggt taatggtaag gatatctaca caactttatc agagcctatt
    12061 cagactgttt tagagactca gatggatgtt ttcgcagaaa aaaccaaagg aaaatttgcg
    12121 agtgctacag ttgtcaacgc taaaacggga gaaattctag caacgagtca gcgtcctact
    12181 tataatcctt ctacccttaa aggttacgat aagaaaaatt tgggtaccta taacacacta
    12241 ctatatgata atttttttga acctggttca acaatgaagg taatgactct agcctctgca
    12301 attgattcta agcattttaa ctctactgaa gtgtataaca gtgcacaata taaaattgca
    12361 gatgcggtta ttcgagattg ggatgtcaat gaagggttat catctggtag ttatatgact
    12421 tttccacaag ggtttgcaca ttccagtaac gttggtatgg taaccttgga acaaaaaatg
    12481 ggacgagata aatggttaaa ctatctttct aagtttaaat ttggttaccc aacacgtttt
    12541 gggatgcttc atgaaagtgg aggtttgttc ccatctgaca atgaagtaac tatcgctatg
    12601 agctcatttg gtcaaggtat tggtgtaacg caagttcaaa tgttgcgcgc atttacgtct
    12661 atctcgaatg atggtgttat gttacaacca cagtttatta gcagtattta tgatccaaat
    12721 acagggactt ctcgtacagc acgaaaagaa gtcgttggaa aaccagtttc caaagaagca
    12781 gctagtaaaa cacgtgatta tatggttact gttgggacag atccttatta tggtacatta
    12841 tacgcagcag gtgccccagt tattcaggtg gggaatcaat cagttgcagt aaaatctggt
    12901 actgcccaaa tcgcccagga aggtggtggt ggttatctgc aaggaaaaaa tgatactatt
    12961 aactcagttg tggctatggt gccatcggaa aatcctgact tcatcatgta tgttaccatc
    13021 caacaacccg aaaaatttag tatcacattc tggaaagatg ttgttaatcc tgttttagaa
    13081 caagcaacag caatgaaaga aacgatatta aaaccggtat tgaatgactc agaacatcag
    13141 acaaaatata aactatcaaa aatagttggt gaaaatccag gtcatgtagc ggaagaatta
    13201 cgtcgtaatc tggttcagcc aattatttta ggaaatggta gtaaagtttc taaagtatct
    13261 aaaagaccag gagctaatct tgctgagaat gaacagctac tagtattaac aaataaactg
    13321 actgaattac ctgatatgta tggttggtct aaagcaaacg ttgaacagtt tgctaaatgg
    13381 acaggtataa aagtgaccta taaaggttca acctcaggta aagtaagaaa acaaagtatt
    13441 gatgttggaa aatccatcaa taaaattaaa aaaatcaaaa ttacaatagg agattaacat
    13501 gtttttaagt ataatggcag gtgtcatagc atttgtcctg acagttattg ccattccacg
    13561 cttcattaag ttttaccaat tgaagaaaat tggcgggcaa caaatgcatg aagatgtcaa
    13621 acaacatcta gccaaagcag gtacgccgac aatgggagga acggtatttt tggttgttgc
    13681 tcttcttgtc tcacttattt ttagtattat tttatctaaa gaaaatagtg gtaatttagg
    13741 tgcaactttt ggtattttat ctgttgttct catctatgga attattggtt ttttagatga
    13801 ttttttgaaa atttttaaac aaatcaatga gggcttgact ccaaagcaaa aaatgtcgct
    13861 ccaactaatt gcgggtctaa ttttttattt tgtccatgtg ttaccaagtg gcacatctgc
    13921 gataaatatt tttggtttta accttgaagt aggatactta tacgcttttt ttgttctttt
    13981 ctgggttgtt ggtttttcaa atgctgtgaa cctaacagat ggaattgatg gtctagcttc
    14041 tatttcggtt gttatcagtt tgattactta tggaatcatt gcttataatc aaacgcaatt
    14101 tgatatttta ttgattatcg ttatcatgat tggtgcttta cttggctttt ttgtctttaa
    14161 tcataaacca gctaaagtct ttatgggaga tgttggttca cttgcattgg gtgctatgtt
    14221 agcagccatt tcaattgcct tacgtcagga atggaccttg ctttttatcg gctttgtcta
    14281 tgtttttgag acgtcttcag tgatgttgca agtagcatac tttaagtata ctaagaagaa
    14341 aactggtgtt gggaaacgta tcttccgcat gacaccattt catcaccatt tagaattagg
    14401 tggtgtgtct ggcaaaggta ataaatggag cgaatggaaa gtagatgctt ttttatgggc
    14461 tattggtatt ttcatgagcg ctattacact tgctatttta tatctttaaa ctttattagg
    14521 ggatgtggta tggactgcca gattcccttt tgctttcttg tagcctttaa tggtataatg
    14581 aacataataa tagtaaggaa atagacatgt catttaaaga ttttaatttt aaaccataca
    14641 ttcagagggc tctcgatgag ttaaaattcg tcgatccaac tgatgttcaa gctaaattga
    14701 ttccagttgt tcgttcaggc cgcgatcttg tgggggaatc gaaaactggt tctggtaaaa
    14761 cccacacctt tttacttcct atttttgaga agctagatga aagtagtgat gatgttcaag
    14821 ttgttattac tgcaccaagt agagagttgg caacacaaat ttatcaggca actaaacaaa
    14881 ttgctgagca ttcagagcaa gaaatacggg tggttaacta tgttggtggt actgacaaat
    14941 tacgtcaaat tgaaaagttg aaagtaagcc aacctcatat tgtcattgga accccaggac
    15001 gtatttatga tttggtgaag tcaggtgatt tagttattca taaggcgcat acttttgtgg
    15061 ttgatgaagc agatatgact ctggatatgg gattccttga taccgttgat aagattgcag
    15121 ggtctttacc caaggatgtt caaattttag tcttttcagc aacgattcca caaaaattac
    15181 agccattttt aaagaaatat ttgaccaatc cagtaatgga aaaaatcaaa acagcaacag
    15241 ttatcgctga taccatcgat aactggcttt tgtccactaa aggacgtgat aaaaatgcac
    15301 aaattttgga actctctaaa ctaatgcagc cttaccttgc aatgatattt gttaatacga
    15361 aagagcgtgc agacgaatta cactcgtatc tttctagtaa tggcttaaaa gtagctaaaa
    15421 ttcatggtgg gattgcacct cgtgaacgta aacgtattat gaatcaggtt aaaaacctcg
    15481 aatttgaata tatcgttgca acagatttag cagcgcgtgg tattgatatt gaaggtgtta
    15541 gccatgttat caatgatgcc atccctcaag acctatcttt ctttgtccac cgtgtcggtc
    15601 gcacaggccg taatggctta tctggtactg ctataacttt atatcagcca agtgacgatt
    15661 ctgatattcg tgaacttgaa aaactaggta ttaattttat tcctaaagtc attaaaaacg
    15721 gtgaatttca agatacatac gaccgtgatc gtcgtaataa ccgtgaaaag tcctatcaaa
    15781 aattagatac agaaatgatt ggacttgtga aaaagaaaaa gaaaaaaata aaaccaggtt
    15841 acaagaaaaa aattcaatgg aaagttgaag aaaagcgtcg caaagagcgt agagcaagta
    15901 atcgtgctaa ggggcgtgcg gagcgcaaag ctaaaaaaca aagtttttaa aattattaaa
    15961 gtctgaaatc agttcgtttc aggctttttt attgatataa aatctttatc acctgataag
    16021 gaaatactat tatacagata gattttagag tgttagacta gtaatatgat attttaggag
    16081 aattcttatg tttaaaaaaa taatattatc tattgcaact atagccgcaa ccgccagcct
    16141 agcagtatca gttcaggcgt cagagaaagt agaacttaaa gtagctacag attctgacac
    16201 ggcaccattt acttatcaaa aagacgggaa attcaaaggt tatgacgttg atgttgtcaa
    16261 agctgttttt aaaggtagta agtacaaagt aaccttcaag acagttcctt ttgatactat
    16321 ttcaacaggt attgatgcag ggaaatttga tttatcagct aatgattttt catataataa
    16381 agaaagagca gaaaaatatc tcttctcaga tcctatatcc cgttcaaatt atgccgtagt
    16441 agggaagaag gggagccatt acaaatcatt aagtgacctc tctggaaaat caacagaagt
    16501 tttatctggc gttaactatg cacaggttct agaaaattgg aataaaaatc atcctaataa
    16561 aaaaccaata aaaatcaaat atgtttctgg gacaactggt gttactagca gattaaaaaa
    16621 tattgagagt ggaaaaattg actttatcct atatgatgcc atttcatctg actatattgt
    16681 aaaagatcaa tcattaaact taagcgtttc tcctttgaaa ggtaaaattg gtaataataa
    16741 ggatggatta gaatacctcc ttttaccaaa agataaaaaa ggtaaaactc tacagaaatt
    16801 tataaataag cgtattaaag ttttgaaaga agatggtact ttggcacgtt taagtaaaca
    16861 atatttcggt ggagattacg tttcaaacat tgataaataa atcagtgaga ctataagttt
    16921 tatcttttta catgtgagag tttgcgtgat agaataacga agataagaaa gtttagaaaa
    16981 ggaaagtaga aggaatgaat caatttatct tgacgggtgg atggtcttgg tataataatt
    17041 tggttagtca ggttccagcg gggaaattat ttagttggaa ggctgtttta gatgcaatac
    17101 cctcaatttt agagcgatta cccataacgc tattattgac agtagcagga gccttatttg
    17161 gactcatttt agctttgatt tttgcagtag tgaaaattaa ccgtgtcaaa attttatatc
    17221 cgattcaagc tttatttgtt agttttttac gtgggactcc aatacttgtt caattaatgc
    17281 tgagttatta tggaattcca cttttcttga aatttttaaa ccaaaaatat ggttttgatt
    17341 ggaacattaa tgctataccg gcttctgtat ttgctattac tgcatttgct tttaatgaag
    17401 cggcttatac aagtgaaact atacgagcgg cgattttgtc ggttgatcaa ggtgaaattg
    17461 aggcagcacg tagtttaggg atgacctcag cgcaagttta tcgtcgtgtt attattccaa
    17521 acgcagcagt agtcgcaaca cctactttga taaacacgtt aattggtcta actaagggaa
    17581 cctctttagc ttttaatgct ggtattgttg aaatgtttgc ccaagcacaa attatgggtg
    17641 gtagcgatta tcgctatttt gaacgttata tctctgtggc acttgtttac tgggcggtta
    17701 gtttcttaat tgaacaattg ggaaatgcta ttgaacgaaa aatggcaatt aaggctccac
    17761 accatttaac tgatgagatt ccaggaggag tacgctaatg ataaaattga gacaattgac
    17821 taaatcattt tctggtcaaa aagtgttaga caaacttgat ttagatattg aaaaaggtca
    17881 agttgttgct ctagtaggtg cttcaggtgc aggaaaatca acctttcttc gtagtatgaa
    17941 ttatttagaa gagcctgatt atggaacaat tgaaattgat gattttaagg ttgattttaa
    18001 atcaatcagt aaagatgaca tactaacatt acgccgtaag ttagccatgg tttttcaaca
    18061 attcaatcta tttgagcgtc gtacagcttt ggataatgta aaagaaggtt tgaaaattgt
    18121 caaaaagatg tcagatcaag aggcaacacg gattgcaaga gacgaactgg caaaggtcgg
    18181 acttgcagat cgtgaaaaat attatccaag acatttatca ggtggacaga aacaacgtgt
    18241 tgctttggca cgcgcattag ccatgaaacc tgatgttctt cttttagacg aaccgacatc
    18301 agcacttgat ccagaattag ttggagaagt tgaaaaatct attgcagatg ctgctaagca
    18361 ggggcaaacc atggtgcttg ttagtcatga tatgaatttt gtttaccaag tagcagataa
    18421 agttttattt ttggaaaaag gtcgtatttt agagtctggg acgcctgaac aattatttaa
    18481 ccatccttta gaagaacgta caaaagaatt cttcgctagt tataacaaat cgtacctata
    18541 agctaaaata ttttgatata atgaaggtta gaatgtctaa tcttcatttt taattattct
    18601 tgagttaggc taagtatata agaaagctgc gatatgtttt gacaatcgct tgttggatag
    18661 agatgttttt agaaatagca aaagtttatt tacaaggttt agggcttgcc agcataactg
    18721 ttattttagt tggtatattt tgggtctttt ggcgtgccaa gaaaaacata gataaaacag
    18781 ctagagaacg ccaatctgtt ctttatgatg tccttctgat agctcttatg acagctccaa
    18841 tacttgcctt tgcatttgta gctatcattt taatgataaa agcttagtca gattagttgg
    18901 aaatatagac gctttgctat agaataaaac cataagattc aaaggagaaa tagtaatgta
    18961 tgatacttta ataataggtt caggtcctgg tggtatgaca gcagcgcttt acgctgcacg
    19021 ttcaaacctt aaagttgggt taattgaaca aggtgctcca ggtggacaga tgaataatac
    19081 tgctgaaatt gagaactatc caggctacga tcatatctca ggtccagaat tatcaatgaa
    19141 gatgtatgag ccgttggaaa aatttgaagt agagcatatt tatggtatag ttcaaagagt
    19201 tgagaacgat ggtgatgtaa agcgtgttat caccgaagat gaatcgtatg aagccaaaac
    19261 agtaatcctt gctaccggag ctaaaaacag ccttttagga gttccgggtg aagaggaata
    19321 tactagtcgt ggcgtttctt actgtgcggt ttgtgatggt gcatttttcc gtgatcagga
    19381 tcttttagtt gtgggtggcg gtgattctgc tgttgaagaa gctgtcttct taactcaatt
    19441 tgctaaaagt gttactatta ttcaccgacg tgatcaattg agagctcaaa aagtacttca
    19501 agaccgagct tttgctaacg aaaaaataaa atttgtatgg gattcagtgg tcaaagagat
    19561 taaaggaaat gagattaaag tatctggtgt aactgttgaa aatctaaaga ctggagaaat
    19621 ctcagagatg actttcggag gtgttttcat ttacgttggt ttgaaaccac attctagcat
    19681 ggtctcagaa ttaggtatta cggacgagac tggctgggtc ttaactgata caaatatgaa
    19741 aacatcaatt ccaggactat atgctattgg ggatgttcgt caaaaagatc tacgtcagat
    19801 tgcgactgct gttggtgaag gtgctatcgc tggacagggt gtttataact atataactga
    19861 gcatttctaa gaaatactaa aagtagaaaa gactatcgaa aaacgattag tctttctttt
    19921 tattttcatg aaagtaaggt gataaactga actaccgaga tagaaatatg ctataatgtt
    19981 agacatatag ccttatccta ggctcatgat taaaaggaga ttgtctgatg tataaagacg
    20041 atagtttaac actacatact gatttatatc aaattaatat gatgcaagtc tattttaata
    20101 aaggtattca caataaaaaa gcggtgtttg aagcttactt tcgcaaggtt ccatttgaaa
    20161 atggttatgc tgtttttgca ggtttagaac gtattgttcg ttatttagaa aatctgagct
    20221 tttctgatag tgatttatcc tatttagaag agttaggtta tcctgaagaa tttttagatt
    20281 acttaaaaaa tttaaaaatg gaactgactg tcaaatcagc taaggaaggt gaccttgtat
    20341 ttgctaatga accattagtt caaattgaag gtcctttagc acaatgtcag ttagtagaga
    20401 cagcgatttt aaatatcatc aactaccaga cacttgttgc tacaaaagca gctcgtattc
    20461 gttctgttat tgaagatgaa ccgctattag agtttgggac acgacgtgct caagaaatgg
    20521 atgcagccat ttggggtact agggcagcga ttattggtgg tgctaacgca acgagtaatg
    20581 ttcgtgctgg taaaatcttt aatattccag tatcaggaac gcatgcacat gctttagttc
    20641 aaacttatgg cgatgattac caggcattta aagcttacgc agaaactcat aaagattgcg
    20701 tctttttagt ggatacttat gatacccttc gtgtaggtgt gcctaatgct attcgagttg
    20761 caaaagagat gggcgaaaag attaatttct tgggagttcg ccttgattca ggtgatttag
    20821 cttatttgtc taagaaagtt cgtcagcagt tagatgacgc cggttttcct aatgcaaaga
    20881 tttatgcttc gaatgatcta gatgaaaata caatcctcaa cctaaaaatg caaaaagcta
    20941 aaattgatgt ttggggtgtc ggaacaaaat taattacagc ctatgatcag cctgcgctag
    21001 gtgctgttta taaaattgtt tctattgaga ctgacgccgg tagtatgcga gatacaatta
    21061 aactatcaaa caatgcggaa aaagtatcaa cacctgggaa aaagcaagtt tggcgtatca
    21121 ccagtcgagc taaagggaaa tctgagggtg attacatcac atttgcagac actgatgtga
    21181 cacaattaga tgaaattgac atgtttcatc caacttatac ttatatcaat aaaacagtcc
    21241 gtgattttga cgcagtaccc ttactggtag atatctttga caaagggaaa ttagtttatc
    21301 aattaccaag ccttcaagaa attcaagagt acggacgcaa agaatttgat cagctttggg
    21361 atgagtataa acgtgttctg aatccacagg attacccagt tgatttggca cgcgatgttt
    21421 ggcaaaataa aatggacctg attgatcgta tccgtaaaga agctttggca aaaggagaag
    21481 taagatgact ttgcaagatc aaattatcaa agaactcggt gtaaaacctg ttattaatcc
    21541 aagtcaagaa atccgtcgtt ccgtagaatt tttaaaagac tatctattaa aacatagttt
    21601 tctaaagacc tatgtactgg gtatttcagg ggggcaagat tcaactttag caggacgtct
    21661 agctcaacta gcagttgaag agttgcgtgc tgatacaggg gaaaactatc aatttatagc
    21721 tattcgttta ccatatggta ttcaggccga tgaagaagat gcacaaaaag cattagattt
    21781 tatcaagcca gatattgctt tgaccattaa tattaaagaa gctgttgatg gccaagttag
    21841 agctcttaat gcagcaggag tagagattac agactttaat aaaggaaata ttaaagctcg
    21901 tcaaagaatg attagtcaat atgcagttgc aggtcaatat gctggagcgg ttattggtac
    21961 tgatcatgct gcagaaaata ttacaggttt tttcaccaaa tttggtgatg gaggtgcgga
    22021 cttattacct ctctttagac ttaataaaag tcagggaaaa cagttattag ctgaacttgg
    22081 tgctgataaa gacttgtacg aaaagattcc aaccgcagat ttagaagaaa ataaaccagg
    22141 aatcgctgat gaaatagcac ttggagtaac ctaccaagaa attgatgctt atcttgaagg
    22201 gaaggtagtt tcggacaagt ctcgaggtat cattgaaaat tggtggtata agggtcaaca
    22261 taaacgtcat cttccaatca caatttttga tgatttctgg aaataagaaa aatagctaga
    22321 atattctagc tatttttgtt tctctaataa attactttca gacttgcaga aatattcaga
    22381 atgtttataa tagtagctaa gtataaaagg aggaatttat gtcagaatta actcaaacat
    22441 ttacagataa gttattagct gattaccaag caaatacaaa atttagcgct attgaaaatg
    22501 cagtaactca caatggttta ttgaagtctc tagagactcg tcaaagtgag attgagaacg
    22561 actatgtctt ttcaattgat ttgaccaaag atgaagtctc taatcagaaa caatcaggac
    22621 gttgctggat gtttgctgca ctgaacacat ttcgtcataa actgatttca gattttaaac
    22681 tcgagaactt tgaattatct caggcacata catttttctg ggataagtat gaaaaatcaa
    22741 attggtttat ggaacaaatt attgcgactg caaatcagga attgagtagt cgtaaggtaa
    22801 aattcctatt agatgtacct caacaagatg gcggacaatg ggacatggtg gttgctctgt
    22861 ttgagaaata tggtgtcgtt cctaaaacag tttatcctga atctgtttca tcaagtgcta
    22921 gtcgtgaact aaatcaatat ttgaataaac ttttacgcca agatgcacag attctacgag
    22981 aattaatagc gcagggagca gatggggcaa ctgttcaaaa taaaaaggaa gaattgcttc
    23041 aagaaatttt taacttttta gcaatgaacc ttggcttgcc accgcaatct tttgattttg
    23101 cttatcgtga taaagacaat cattatcagt ctgataaaaa tattacaccg aaagcatttt
    23161 atcaaaaata tgttaatctt gacatatcag attatgtttc tattatcaat gctccaactg
    23221 tagataagcc ttatggacaa tcctatacag ttgagatgct tggaaatgtt gtcggaggcc
    23281 cagctgttaa atatttaaac cttgatatga aacggtttaa agagttagcg attgcgcaaa
    23341 tgaaatcagg agaaactgtg tggtttggtt cagacgtagg tcaagtatca aatcgtcaaa
    23401 agggtatcct agcaactacg acttatgatt ttaattcatc tatggatatt aaacttagcc
    23461 aagacaaagc gggccgccta gattatagtg aaagtttaat gacacatgct atggtcttaa
    23521 cgggagtaga ccttgatgaa tctggacaac ctttaaaatg gaaagttgag aactcttggg
    23581 gagagaaagt tggtaaggat ggttattttg tagcttcaga tgcttggatg gatgaatata
    23641 cttaccaaat agtcgttcgt aaagaattgc ttactaaaga agaattagag gcttacaacg
    23701 cagagccaat tactttagcg ccgtgggatc cgatgggagc actagctaat tagtaatagt
    23761 aattataaat taaaaaactg gatatattat ccagtttttt taattaccgt taggtgctgt
    23821 attgttatta ccattaccat gtccagttcc tggcacagtt tgattggaat tattaggggt
    23881 agtagcatta ccattattag gtgtttcgac ttgtggggat gatgaattgg catcatctgc
    23941 tgtattctta tcctcttttt gtttctcaga ggatgttgaa ctactgcttt gacttgactg
    24001 tccagaattt tgatagatat tcttatagac agaagaagag taagtgccgg ttgtagttgt
    24061 cccattgata tacaagtagc ttccacttcg atagagccct tctggcattg tccaatctgc
    24121 actgtatccg ccagttaggt aggacatcat tgcacgataa acctcagtag caatatccag
    24181 ttggctacca taaagtggtg taaggcgatt tttataacca gtccaaattg ccattgtgta
    24241 cttagaagta tagccgacaa agttttcgtc aggagccatt gtaccaacgg cgctattgta
    24301 gataccagta gttgcttcaa ttttagctaa ctcatcttct gtatagttgg aagtaccagt
    24361 cttaccagct tgtgcaacac cagggatagc tgctttagta ccagtaccaa atgttagtac
    24421 tgttttcaac atatccgtca tcatgtaggc agtagtctct ttcatcgcac ggctaccaga
    24481 cgctgcataa gtatcattgg ttccatcgct aaattcaatt ttattaacat attgcggctt
    24541 atagtaagtt ccgccgtttg caaaagccga gtaagccgct gccattttct cactacttgc
    24601 accatatttt gcatcactgc tattgttgtt acttgaaata gcatttgaat agttcatttc
    24661 tggatagtat atgcctaatt tttcaaggaa agattttgct tcgtctaatc cagcggcttc
    24721 aagtgctctg acagcaggga cgttacgtga ttgttgaata gcggtctgca tactcatcca
    24781 acccatatat tgacgatccc agtcatatag ttgagtagaa gtaccaggcc agtagtaaac
    24841 tgagtcgttt aatgattgac ctgttgaatt atagacacca ctatcaatag caggtgcata
    24901 agctgagata ggtttcattg tagaacccca atcgcggtct gttaagacag attgatttgt
    24961 cccaaatgaa atattttcat tctgatgacg cccgcctaat tgtgcaatga ctttaccatt
    25021 agtcgcatcc atgatggtag atgctatttg taattcattg tttggataag cgatgtaagt
    25081 atcactgttg tagatgtcat atagttgttt ttgtgcatca gtgttgatat tagtatacac
    25141 ttttagccca gcagtaaaga tatctttacc agttttttgt ttaacttcac taataacttg
    25201 ttttaagtag ttatccatat attttggata agtagatttt tgctttaatt cttttaagcc
    25261 atcagttact ggagttgcaa cagcttggtc gtattccttt ttagaaatgt ttttatcttg
    25321 atacatctgc tgaagaacgg tattacgtct tgtttgagca gattctgggt ttttataagg
    25381 gtcatattgt gtaggtgctt gaggaatacc agcgagcaaa gcaagttgtg caatagataa
    25441 ttcctttagg tctttaccaa agtatgattt agctgttgtt ctcataccat aattcccatt
    25501 tcccatataa actttattaa tatagaaagt aagaatttct tctttggtgt atttacgctc
    25561 catttgaagc gcaagccaaa cttcctgtga tttacgtttt aacgtttggt cagatttatt
    25621 ggtagaaaag taagccagtt taatcaactg ttgatcaagg gttgaaccac cttgcgtatt
    25681 actactaaca aggttatgcc aagctgcacc taaaatacga taaatatcga cacctctatg
    25741 tttaaagaaa cgtttatctt ctatagaagt gatagcgtta accaaattta gtggaatgct
    25801 atccgcacta acactttcac gcttttctga gcctaaatcg gcaataagtt tattattacc
    25861 atcataaacc aaactagagt taacggattt taaagcttga tcggttaatt tcggagaacg
    25921 actaacgtag tatgcaaata ggagcccacc tattacaata gctaagataa taaatcccat
    25981 tataatgcca aaagcatact tcaatagttt gataacagat tcctttttaa ttgtaatcac
    26041 cgcctaataa tttttcttca ataatatcta agtaagggac ttgaggaaag gcactctcct
    26101 ttacgaaaaa tccatttttt ctgatataat caataggcat tgatttattg cctttatcaa
    26161 tctgataaaa tgaaattaac tcattagcag ggagtagata ggtttcctta agtgtggaaa
    26221 aatgaagcaa gacaaagcaa atcccttttt gctgtaatac atttgccatg tgctctattt
    26281 ggtgagcatg aaaatttttc ataggcatag cagttttctg ccgggtttct ttggcttcaa
    26341 agtcaatata gtaacctttg taaacaccag aataatcagt agtagaagct tgtctaaaat
    26401 aagcttctac aatcttggca cgactacgtt taggataatc taccttgacg atttgtactg
    26461 gcgttggttt cttatgaatg acagctaatt cgtgagacaa atagtagtca ttggttgcat
    26521 tgatagcagc ttcaaatgac ataccacggt tggcaaaatc gattttcttt ttctttgatt
    26581 ttgtaactgt tgttttccga ataagctgat gtggatagtt aaccattttt ctccttgcat
    26641 tacaaataaa atgctaagat aagcaaaact attataccat aaaattagga tggacaacaa
    26701 atgaagtcaa ctattcttgt aacaggttac aaaaattttg aattagggat tttccaggat
    26761 aaggatcctc ggattactat tataaaaaag gctattgata aggattttag acgcttcttg
    26821 gaaaatggag cagactggtt tatttttatg ggaaatttag ggtttgagta ctgggctttg
    26881 gaagttgctc ttgatttaca aaaagaatat gattttcaaa ttgcgacaat ttttaccttt
    26941 gagaaccatg gccaaaattg gaatgaggct aataaagcta agttggcact ctttaagcaa
    27001 gttgactttg ttaaatatac tttcccaagc tatgaaaacc cgggtcaatt taaacaatac
    27061 aatcattttc tcattaacaa cacacaaggt gcatatcttt tttatgattc tgaaaatgaa
    27121 actaatttaa aatttttgct agaaatgatg gaaaaaaagg aagcttatga tatttctttc
    27181 ctaacttttg atcgcttaaa tgaaatatat gaggaatgat cgttttttcg gatatgagaa
    27241 cgttttacgc ttgatttttc catttaattt aggtattatt tactttgata taaaggatag
    27301 gacttatatt aaaacgattt gactggtttg gagaatagaa tggcaagtat tatttatagc
    27361 ccaaaagata tttttgagca agattttaaa gttagtatgc ggggatatga caagaaagaa
    27421 gtcgatgtat ttctggatga tgtcatcaaa gattacgaaa attatcttga gcaaattgag
    27481 aagctccaaa tggaaaatag acgtctacaa caagctttag acaaaaaaga aagcgaagct
    27541 tcaaatgtaa gaaattcagg gacagctatg tataatcaaa aaccgattgc ccaatctgca
    27601 accaattttg atattttaaa acgcattagc cgccttgaga aagaagtttt tggaagacaa
    27661 attagagaat aaggatttct caaatatgca atttttggat aatcgcgtag tattgatata
    27721 ctatgaggaa agtccatgct agcactggct gtgatgccag tagtgtttgt gctaggcgaa
    27781 aaaataagcc tagggagata gctagctatc ttacggcaag caaaagggct aagtctttgg
    27841 atatgcctga atagctttga aagtgccaca gtgacgtagt ttctagggaa atctagaaag
    27901 tggaacgcgg taaacccctc aagctagcaa cccaaacttt ggtaggggca tgggatagtt
    27961 ggaatgagaa caatctatcc tgactgcttt gcagtagaca gatgattatc gaaagaaaca
    28021 attcctagtt gtttctggaa caaaacatgg cttatagaaa attgcataat aggttagtgg
    28081 ctgagataac atctcagcca atttttaact gtgatgaata cgatagtgta aaagaggaaa
    28141 aatgaaagaa tcattcaaat taatagcaac tgctgctgct ggtttagaag ccattgttgg
    28201 tagagaaatc agaaacctag gaattgactg ccaagttgag aacggtagag tccgtttcca
    28261 cggagacatc aaaacaatca ttgaaacgaa cctatggcta cgagctgccg atcgtataaa
    28321 gattattgta ggagagtttc cagcacgtac ttttgaggaa ctttttcagg gtgtatatgg
    28381 ccttgactgg gaaaattatt taccgttagg ggcaaaattt cctattgcta aagcaaagtg
    28441 tgtcaagtca aaattgcaca atgaacctag tgtacaagcc atttctaaaa aagctgttgc
    28501 taagaaactt caaaaagtct ttcaccgtcc tgaaggagtt cccttacaag aaaatggagc
    28561 tgaatttaaa atagaagttt ctattttaaa agataaagcg actgtcatga ttgatacgac
    28621 tggatcaagt ctcttcaaac gtggttaccg agcagaaaaa ggtggtgcgc caattaaaga
    28681 aaacatggca gcagctatta ttcaattatc taattggttc cctgataagc cattgattga
    28741 tccaacttgt ggttcaggta ctttttgtat tgaagccgct atgattggaa tgaatatagc
    28801 tcctggtttt aatcgtgatt ttgcctttga ggcgtggcca tgggtagatc aaagtcaagt
    28861 acaaaaggtt cgcgatgaag ctgagagtaa agcaaactat gatattgatt tagatatttc
    28921 aggttttgat ttagacggtc gtatggtaga aattgctaga aaaaatgcag aagaagcagg
    28981 acttggagat gttatcaaat tgaaacaaat gcgtcttcaa gatttaaaaa cagataaaat
    29041 taacggtgtt attatctcca acccaccata tggtgaacga ctcctggatg acaaagcagt
    29101 ggacattttg tataatgaga tgggacaaac atttgcgcct ttaaaaactt ggagcaagtt
    29161 tatcctaact agtgacgaag gatttgaaaa aaaatacggc tcacaagctg ataaaaaacg
    29221 taagctttac aatggtactt taaaagttga tttgtaccaa tattacggtg aacgtgtgag
    29281 acgtcaagta aaataacggg gagaactcat gtcagaagat caaaaacacc cattttttga
    29341 accagaaaaa gaaactgatg gtcttgaatt taaagatgct aaagaaatga cagtagaaga
    29401 ggctgttaga aaagattctg aaattaaggc tggtattacc gaagaagata gtattcttga
    29461 taaatacatc aaacaacatc gtgatgaagt agcttcacaa aagtttgaga caaaatcaag
    29521 cgattttgca aaccttgata cggcatcttt agatgatttt atcaagaaac agcgcgaaga
    29581 attatcagcg atgttagctg cagaagagct ttctaaaaag ttggataatt cagtctctca
    29641 agaacaagat atagaagcta atgctgtctc acctaaagaa gaatcttcac aagaacaaga
    29701 aaatagtgta actccagttc ctcctctgaa tacggaagca gaaccaactg caacagaacc
    29761 agattcgact attgctgatt cagaggagta taaatcctct tctaagaagc gtggaggtat
    29821 tgtaggaaca ttgattgctc ttattttact tctaatagta acaatctttg gttacaacta
    29881 tttcaagaat aataattcga caaattctca aactgcaaca agtcaaagtt ctagttctaa
    29941 ggctacgacg acttcatcag aagaagataa aaaagctagt cagaacttag ataatttcaa
    30001 caaagcttat gctaactttt ttgttgatga taagaaaacc caactcaaaa atagcgaatt
    30061 tgataaattg tcagaattag aaaagaaagt tgatgcttta aaaggaacta aatactacgg
    30121 taaagtaaaa gttaaatttg atagtctaaa acgtcaaatt gatgctgtta aagctgttaa
    30181 cgacaagttt aagtcgcctg ctgtagttga tggtaagaag agtgaaaaat tagaagtaaa
    30241 agatggagcg aattttgact ctttagatag taaaactctt aatactggta atgctagtct
    30301 agatagttta cttcactcca tcgtttcaac tggtcgtaat caggtaaaac aatccgaaga
    30361 acaggctaaa aaaactcaag aagaacagtc ttcgtctaat aaggtttcag acactcaaac
    30421 aactgaacaa ccaaacgtaa ctaacggtca atcttcctca tcagctgcta caataaataa
    30481 tcaggcagct ggcactgcat caggtaattt ggaacgtaac cgtagccgag taccgtataa
    30541 taatgctgct attgcggaca caggaaatcc tgcctggata tttaacccag gagtattaga
    30601 gaaaatcgtg gccacttcac aggcgcgagg ctatttctct ggaaataact atattcttga
    30661 acctgtaaat attatcaatg gtaatggtta ttacaatatg tttaaattag atggcactta
    30721 tttgttctct atcaatgcca agacaggtta ctttgtaggt aacgctcctg gaagagctga
    30781 tagtttagat tattaatata aagagcaaaa ccagtttttt gtctacaccc taaaagttag
    30841 aaaatcagtc taacgattag ggtgtttttt gtattcgcat ttagatgtgt acgactgcta
    30901 tgaggatatt agggaaaaat taaaaggatt ttgctctcta tgatacagaa caaaatcctt
    30961 tgtttaaatt aataagacaa cttttggaat tccaaaagtt gacttttatt ttttattaga
    31021 tcagacaatg tggcgctcaa aagcattgtc agaaatccct tgggaaagaa ttaatttcgc
    31081 ccattcttga gcagagtgga gactgtggtc tttataattt ccacaagatt caatggttgt
    31141 tccaggaacg tcttcccaag tgaccccacc agcaatagcc tctagactag atttgatgac
    31201 tttagcaatt tcagtagcat cctgttttcc ccacataatc atgtgaaaac cagttctaca
    31261 accaaaagga gaacaatcaa tcaaaccatt aatttgttgg cggattaact tcgccagaag
    31321 gtgctcgata gtatgtaaac cagcagtatc aatagcattt tcattaggct gaataaggcg
    31381 gatatcaaag ttagtaatga tgtctccgac cggtccaact tcttctgaaa ttaaacgaac
    31441 gtagggtgct ttaacgatgg tgtgatctaa ttcaaaactc tctacgacaa cttcttttgt
    31501 cataagtttt ctccttttgt attacattct agagactaaa ggagatgttg aaaatttctc
    31561 ctaatccctt taaataacat tctaatcata tcataaatgt ctataatttt aaattgtgaa
    31621 aacgcctttt ttatgataaa cttaaaagtg gactttctat atgaaatcta gaaaaaaatt
    31681 gaggtataaa catgtttaat attatattag caatggtttg tgcactcatt ggtttgatta
    31741 ttggttatgt ggctatttca atgaaaatga aatcatcaaa agaagctgca gaactaactc
    31801 ttttaaatgc agaacaagat gccgttgatt tacgtggtaa agcagaaatt gaagcagaac
    31861 acatccgtaa agcagcggaa cgtgaaagta aagctcacca aaaagaatta cttttagaag
    31921 caaaagaaga ggctagaaaa tatcgtgaag agatcgaaaa ggaatttaaa tctgatagac
    31981 aagagttgaa acagatggag gcacgtttaa cagatcgtgc atcatctctt gatcgtaaag
    32041 atgaaaatct atctaacaaa gaaaaaatgt tagatagcaa agaacaaagt ctaaccgata
    32101 aatctagaca tattaatgag cgcgaacagg aaattgctac tttagaaaca aaaaaagtag
    32161 aagaattgtc acgcatcgca gagcttagtc aagaagaagc taaagatatc attttagctg
    32221 ataccgaaaa agatttagct catgatattg cgacacgtat taaagaagca gaacgggaag
    32281 taaaagatcg atcaaacaag atagcaaaag acttgctagc ccaagctatg caacgtttag
    32341 ctggtgaata tgttactgaa caaacaatta caactgttca tctccctgat gataatatga
    32401 agggacgtat cattggtcgt gaaggacgta atattcgtac tctggaaagt ttgacaggta
    32461 ttgatgttat catagatgat acgccagaag tcgttgtctt atcaggtttc gaccccattc
    32521 gtcgtgaaat tgctcgtatg actttagaat ctttgattca agatggacgt atccatccag
    32581 ctcgtattga agagttggtt gaaaaaaatc gtttggaaat ggatcaacgt atccgtgagt
    32641 atggtgaggc agcagcttat gagattggtg caccaaatct gcatcctgat ttgataaaaa
    32701 ttatgggacg tttgcagttc cgtacatcat atggtcaaaa tgtacttcgt cattcagttg
    32761 aagttggtaa acttgcaggt attttagctg gagaactagg agaaaatgtt gatttagctc
    32821 gtcgggctgg cttcctccat gatatgggga aagctattga tcgcgaagtt gaaggcagtc
    32881 acgttgaaat tggtatggaa tttgctcgta agtacaaaga gcatccgatt gttgttaata
    32941 ctattgcaag ccaccatggt gatgtagaac ctgacagcgt tattgcagta attgttgctg
    33001 ctgcagacgc ccttagttca gcacgaccag gtgcgcgtaa tgagtcaatg gaaaattata
    33061 tcaaacgtct gcgtgattta gaagaaattg ctaatggctt tgagggagtg caaaatgcct
    33121 ttgcacttca agccggacgt gaaattcgta tcatggtaca acctggtaaa gtttctgatg
    33181 atcaagtagt tattatgtcg cataaggtac gtgaaaaaat cgaacaaaac ttggattatc
    33241 caggtaatat taaggtaaca gttattcgtg aaatgcgagc tgttgatttt gcaaaatagt
    33301 caaaaggaga gcttgttagc tctccttttg ttatagagtt gacatttgtc atattttgaa
    33361 atgacaaaaa tatctaaaaa gtttccttct gattttttat actatgatta tagtaaagga
    33421 gaaccaaatg gcaaaagata taattaaggt tgatcatata tttaaaagca tcgggcaaaa
    33481 aacgatttta gaagatatat catttagtat agcttctaac caatgtgtag cattgattgg
    33541 acctaatggt gcagggaaaa caactctgat gtcaacttta ttaggtgata ttagtatttc
    33601 ttcaggagca ttgactattt ttaatctgcc tgcacaccac aataggttaa aatataaggt
    33661 ggccatctta ccacaagaaa atgtattgcc ctcaaagttt acagtgagag aactcattga
    33721 tttccaaagg tgtttatttc cagaagtgct gccaatgtct ctgattttag actatctaca
    33781 gtggagtgat gcgcacttgc aacaatttac agagacttta tctggagggc aaaaacgact
    33841 tttagctttt gttttaactt tggttggtaa gccccaatta ctatttttag atgaacctac
    33901 ttccggaatg gatacttcca cacgtcaacg attttgggag ctggttgcga cactaaaaaa
    33961 agaaggtgac acaattgtct attctagtca ttatatcgaa gaggtagaac atacagctga
    34021 taggatttta gtacttcata aaggaaagtt attacgcgat acaaccccct ttgccatgaa
    34081 gcaagaaaaa accgaaaagt tattcaccgt tccgcttagt tatcaaaaat tattacctac
    34141 ctatttgatt acagagtgtg aagccaagag tgatagtata acgtttgtta ctggggaggc
    34201 tgaaactgta tggaaaatac tggcagataa tggttgtcct attgaagcta ttgagatgac
    34261 caatagaact ttgttaaatc gtatttttga gactactaag gaggtaaaac atgagaatct
    34321 ttaaagcatt attgaaaatg gaatttcttt tgacaaaacg tcaacttgcc aatctcatta
    34381 tggctatagg tatgccagtg gctttttttc tatttttttc aggatttatg ggcgaaggac
    34441 tcactaaagc catcgaagct atctacgttc gtaattatat gataacgatg gcaggattct
    34501 caagtcttag ctttgctttt tttacttttc cattttctat gaaagatgat caactctcaa
    34561 accgaatgca attattaaga cactcaccag taccaatgtg gcaatattat ctagcaaaaa
    34621 ttattcgtat tttattttac tattgcctag caataactgt tgttttccta acagggcata
    34681 ttttaaggca agttagtatg ccaatagaac aatggatgca gagcttttta ttgctattag
    34741 gaggagcaac atgttttatt ccttttggat tgctcgtctc ctactttaaa aatactgaac
    34801 taatgtcaat ggtagccaat atttgttata tgtcattagc tgttcttggt ggcatgtgga
    34861 tgccaattac aatgttccca aaatggttgc aaggtttatc aaagttaacc cctacttacc
    34921 atttgactca agttatcctt agtccgtttg ctaatagttt tgctgttttt tctcttatta
    34981 ttctgatagg ctatggtatt ataatgttag ttatcgctta tctactatct cagaagaggc
    35041 attcaatttg aaaaataaaa aaaataacta tgatttaatg tattttattc cgttagtatt
    35101 tttgatttac cccataggag ggattctcta ttatcactac cctttttgga ctttattttt
    35161 tacgctagct tttgtaggag catatttata ctctattatt atcaggggcg aatcaaagta
    35221 tcacatgatt gcctggttta caatgttgac atatattttc tacatgacag tttttataaa
    35281 tagtggtttc atctggtata tttactttct gagtaattta ctcgtctatc gctttagaga
    35341 taaattgaag tcttttagat tcattagttt tgcttgcact ctagccacaa tggtattttt
    35401 atgcttcttt aaggcatcag actttggtga tagaatcatg tttctaattg ttcctatttt
    35461 ttgtatcggt tacatgtgga ttgctattga aaatagaaat agcgaagaac aaagagaaaa
    35521 aattgcagag caaaatcagt atatcaatat tttgtcagct gaaaatgaac gtaaccgtat
    35581 agggcgtgat ttgcatgata gtttaggaca tacatttgct atgatgacac taaagacaga
    35641 actagcgtta aagttgttag aaaaaaggaa ctatgataag gtacaaaaag aactatcaga
    35701 attaaatcat attagtcatc aatcgatgag tgaggtccgt caaattgttt ctaacctcaa
    35761 gtatcgtaca gtagtggaag aaattgatga gttgtaccgc ttgtttcaat tatctaacat
    35821 caagttaaca gttgttaata agttagagac gtcacaacta agtccagtaa cacaatctac
    35881 aatcacaatg atattaaagg aattatccaa taatattgtt aagcatgctg aagctgatag
    35941 cgtggagttg agtttagtaa ggcaaggtgc tactataaat attgagatga ttgataatgg
    36001 ttgtggtttt acaaaccttg acggagatga gttgcatagt attcaagaga gattaactat
    36061 cgttgaaggg acactaacga ttttgtcccg aagtaaacca actcatattc aggtgatatt
    36121 gaaagaggta taagatgaaa ttacttgttg ctgaggatca gtccatgcta cgagacgcta
    36181 tgtgccaatt acttttgatg gaagaaagtg tttctactat tgatcaagct ggaaatggtg
    36241 aagaagctat tgccatttta tccaataaag ccatagatgt tgcaatattg gatgttgaga
    36301 tgccgatatt atcaggttta gatgtcttag agtgggttag aaaataccag aatgttaagg
    36361 tcataattgt aacaaccttt aagcggtcag gttatttcca aagggctatt cgttcaaatg
    36421 tagatgctta tgttttgaaa gaccgctcag tagctgattt gatgaaaacg attcaaaaag
    36481 ttttatctgg gggtaaagaa tattcgcctg aattaatgga aaacgttata tctaatcctt
    36541 tgagtaagca agaaataaaa attctttccc taattgctca agggaagacc agcaaagaaa
    36601 ttgcatgcac tttattttta tcgaatggta cagttcgtaa ttacacgtca aatatttttg
    36661 ataaattagg agttaataat cgtttggagg cacttaaaat agcacgagaa aaggattggc
    36721 tgtgatagtt tttttccagt cgagatgcca taatgtatta agagggatat atgtatgcaa
    36781 aaatttaaag aacgttttga tacgcttagt gatgcaattt tggccattgc tatgacgatt
    36841 ttagttttgg agataaaaac tccgactacg atgggtgata ttggtgactt tacacggaac
    36901 ataggtttat ttattgttag ctttgttgtt gtttttaatt tttggtacga aagggcacaa
    36961 aatagcctag atgctcaaaa gactaatgat gaaattatag ctcttgatat cattgaacac
    37021 ctaggtattt gtttgattcc tctctttact aaatttatga tttcttttga gaatcataat
    37081 tttgctgtta tggcttatgg actcttaacg ttattagttg gtctcacatc agacattatt
    37141 cggattcgtt tggcaagcta tgatttagtg accattccta gtgaattgaa agagcgggtt
    37201 ataaaagtca tgacaacttt tgccattcgt tcagttgtgg ttcgctttat cattattatc
    37261 ctcgcttatt ttctacctga ggtagggata tttgcatatt tagtcatacc gctctttatg
    37321 ttttggcgtc gctctagagc aggaaaatac tatactaaaa agggcattga ggcaccctct
    37381 tatatcaagc tttttatcca aattggagag agagttcctg aggattttac taattcaagt
    37441 agtggaataa gtggggtaaa aagactctat aaaaaataat aggagacctt gaaaaaaagg
    37501 tttctttttg ttaaactaat gagaagaaac tttatttagg gagcaattta tgaattgcta
    37561 taaaagttaa tagttaaggt aagaaccaga ttgttctggt ctttttaaca cttaacaccc
    37621 gatctattaa tttgaggagt gtgtaatgtc tgaacgtggt ttgctaattg ttttctcagg
    37681 accatctggt gttggtaagg ggacagttag gcaagaaatt ttttcaaccc ctgaccataa
    37741 atttgattat tccgtttcaa tgactactcg tccacaacgc ccaggcgaag ttgatggagt
    37801 tgattatttc ttccgtacac gtgaagagtt tgaagcgttg attaaagaag gtcaaatgct
    37861 tgagtatgcg gagtatgttg gtaactacta tggaactcct ttaagttatg tcaacgagac
    37921 tttagacaaa gggattgatg tttttcttga aatagaagtt cagggagcgc ttcaagttaa
    37981 atcgaaggtt ccggacggtg ttttcatttt cttaacacca ccagatttag aggaattaga
    38041 agagcgttta gtaggacgtg ggacagatag tccagaagtg attgcccaac gtattgaacg
    38101 tgccaaagaa gaaattgctt tgatgaggga gtatgactat gctgttgtca atgatcaagt
    38161 gtcattagca gcagaacgtg ttaaacgtgt cattgaagca gaacattatc gtgtggatcg
    38221 tgttattggg cgttatacca acatggtcaa agaaactgat aaaaaattaa gttaaaaata
    38281 gaaaagagaa ttgccttatg atgttaaaac cttctatcga cactttgttg gataaagttc
    38341 cttcaaaata ttctttagtt attttacaag ctaagcgtgc tcatgaattg gaagctggtg
    38401 agaaagccac tcaagatttt aaatctgtga aatcaacact tcgcgcttta gaagaaatcg
    38461 agtcaggtaa tgttgtaatt cacccagatc catcagcaaa acgtgcctca gttcgcgctc
    38521 gtatcgaagc tgaacgcctt gctaaagaag aagaagaacg taaaatcaaa gagcaaattg
    38581 ctaaagaaaa agaagacggc gaaaaaattt aagcaagtca aaagtgagag gttgaaacat
    38641 tctgtatcag cctcttttca tattttagag aattagggag ggtttatggc aagaaaatta
    38701 gcacaggtta ttgttgatat tcctctgatg cagacagata aaccattttc gtatgccatt
    38761 cctaaagatt tggaagattt ggtacaagta ggagcacgcg ttcatgttcc ttttggaaga
    38821 gggaatcgtc ttttgcaagg ctttgttgtc ggtttcagag atgatgatga attagagact
    38881 aaggatattg cgggggtcct agattttgaa cctgttttaa atcaggaaca actagactta
    38941 gctgatcaga tgcgccatac tgtcttttct tataaaatat ctattttgaa atcaatgtta
    39001 ccaagtcttt tgaattcgca atatgataaa ctcttgttag caacagacac attaccatca
    39061 gaagatagag aagatctatt tggtcataaa acggaaattg tcttttcaag tttgtcttct
    39121 caagatgcca aaaaagcagg cagattaatt caaaaaggtt tcatagaagt tcaatattta
    39181 gccaaagata agaaaacaat aaaaacagaa aagatttaca aaatcaacag aactctgtta
    39241 gaagagagtc aaatagcagc aagagcgaaa aagagactgg agttgaaaga attcctgtta
    39301 gaaaatcctc aaccagggcg cctcacagct ttaaacaaac aattttcatc tcctgtagtt
    39361 aattttttca gagaagaagg aatcattgag gtcattgaaa aagaagctag tagatctgat
    39421 aattatttta aaggaatcct aaaaacggat tttttggatt taaatcagga acaagctaaa
    39481 gtagttaaaa tagttgttga ccgaattggt aaggatcaga ataaaccctt cttgttggag
    39541 gggattacag ggtctggtaa gacagaggta tatcttcatg tcattgataa cgttttaaag
    39601 ttgggaaaaa cagcgattgt tctcgttcca gaaatttctt taactcccca gatgacgaat
    39661 cgttttattt cccgttttgg aaagcgagtt gctattatgc actcgggctt gtcagatggt
    39721 gaaaaatttg atgagtggcg aaagataaaa tcaggtcaag ctaaagttgt cgttggagct
    39781 cgttcagcaa tttttgctcc tttggaaaat atcggagcca tcattataga tgaagaacat
    39841 gaatcaactt ataagcaaga atctaaccca aggtatcatg cgcgcgatgt ggccttgcta
    39901 cgtgcagaat atcataaagc tgtgttacta atgggatcag caactccaag tatcgaaagc
    39961 cgagctagag ctagtcggga tgtttataaa tttttagaat taaaacatcg tgctaatcct
    40021 aaagcacgta ttcctcaggt tgaaattatt gattttagaa attttattgg tcaacaagaa
    40081 gttagtaatt tcacaccata tttactagat aaaatccgag atagattaga taagaaagag
    40141 caagtggttc ttatgctcaa tcgtagaggt tattctagtt ttattatgtg tcgaaactgc
    40201 ggctacgttg atcaatgtcc taattgtgac atttctctga ctttacatat ggctacaaag
    40261 accatgaatt gtcattattg tgggtttgaa aagcctattc caagaacatg tccaaattgc
    40321 aatagtaaaa gtatctctta ctatggtact ggaacccaaa aagcttatga agaacttttg
    40381 aaagttatcc cagacgcaaa gattttacgg atggatgttg atacgacaag gcaaaaaggc
    40441 ggtcatgagt ctattttgaa gcgtttcgga aatcatgaag ctgatatttt attgggaaca
    40501 cagatgattg ctaagggatt ggattttcca aatgttactt tagttggagt actaaatgca
    40561 gatacttctc tgaatttacc tgattttcga tcatcagagc gaacttttca gctattaact
    40621 caagtggctg gacgtgctgg gcgtgctgaa aaagaaggag aggttgtcat tcaaacttat
    40681 aatcctaatc attatgctat tcaattagcc caaaagcaag attttgaagc tttttatcaa
    40741 tatgaaatga atatccgtcg acaacttggt tatcctcctt attattttac agttggttta
    40801 acattatcgc ataaagatga agagtggtta atacgaaaat cttatgaagt attaagttta
    40861 ttgaagcaag gctttagtga taaagtaaag ttgttaggtc caacgccaaa accaattgca
    40921 agaacacata acctttatca ctaccaaatt attatcaagt atcgatttga ggacaacctt
    40981 gaacttgttt tgaatcgctt attagacatc actcaagaca aggagaatag agatcttcgc
    41041 ttagcaatag accacgaacc gcaaaatatg atgtagaata gtgctatgat ataattgaaa
    41101 aaagaaagac gaggaaaaag caatgacaaa attattattc atgggtaccc ctgatttttc
    41161 agcgacggtt ttaaaaggta ttttagcaga tggtaagtat gatgttttag ctgttgtgac
    41221 gcaaccagat cgtgctgtag gtcgtaaaaa agaaattaaa atgacccctg taaaagaagt
    41281 ggccttggaa aacaacattc ctgtgtatca gccggaaaaa ctatctggaa gtccagaatt
    41341 agagcaacta atgaccctag gtgcagatgg tattgtaaca gctgcttttg gtcaattctt
    41401 accaacaaag cttttggaat cggtaggttt tgcaattaat gtacatgctt ctctattacc
    41461 aaaatatcgt ggtggggcac caattcatta tgccattatt aatggcgaaa aagaagctgg
    41521 ggttaccatc atggagatgg ttgcaaagat ggatgcaggg gatatggtat caaaagcttc
    41581 cgttgagata actgatgaag acaatgtagg gacaatgttt gatagactcg ccgttgttgg
    41641 tcgtgacctt ttattagata cgcttccagg ttatctatca ggtgatatta agccgatacc
    41701 acaaaatgaa gaagaggtaa gtttttctcc taatattagt ccagatgaag agagaattga
    41761 ttggaataaa tcatcgcgcg acatttttaa ccatgttcga gggatgtatc catggccagt
    41821 tgcacatacc cttttggagg gaaatcgttt taaactttat gaagtaacaa tgtcagaagg
    41881 aaagggctca ccgggtcaag tgatcgctaa gacaaagaac tcattgacag tagcaactgg
    41941 tgacggagct attgaactca aatcagttca accagcaggg aaacctcgta tggatataaa
    42001 agacttttta aatggtgttg gtcgtaattt agaaatagga gataaatttg gcgaatgatt
    42061 ggaaaaagag tgcgcgtggt ttagctttaa tgactttaga agaagttttt gataaaggag
    42121 cttattctaa tatcgcactt aataagagtt taaaaaaatc tagactatca gataaagatc
    42181 gtgccttagt aacagaaatt gtctatggaa ctgtggctag aaaaataacg ctagaatggt
    42241 atctatcaca ctttatagtt gatcgtgata agcttgaatt atgggtatat catctcttgc
    42301 tactgagtct ttatcaacta ctctatctgg acaacatccc agatcacgct attgttaatg
    42361 atgctgtcac tattgctaag aatcgaggca ataaaaaggg tgctgaaaag ttaattaatg
    42421 ccgttttacg aagagtaagt tcggagacgc tccctgaaat tgcatcaata aaacgccaaa
    42481 acaaacgtta ctctgttgct tattccatgc cagtgtggct cgtaaaaaaa ttaattgacc
    42541 aatatggtga gacaagagct ttagcaatta tggagagttt atttgagcga aataaagcca
    42601 gtcttcgtgt gactgactta tcgcaaaaac aaactattaa agaaacttta aatgttagag
    42661 attcacatat tgcagagaca gctttagttg cagactcagg aaattttgca tcgacatcat
    42721 ttttccaaga tggcctcatt actatccaag atgaatctag tcaattagtt gctccaacac
    42781 tcaaagtctc gggtaatgac caagtattgg atgcatgtag tgcaccagga ggaaaaacaa
    42841 gtcatatcgc ctcatatttg actacagggg cagtaactgc tttagatttg tatgatcata
    42901 aattggaatt ggttatggaa aatgcaaaac gcctaggtct gtcagataag ataaaaacta
    42961 aaaagttaga tgcaagtaaa gctcatgagt attttcctga ggatactttt gacaaaattt
    43021 tagtagacgc accttgttca ggtatcgggc taattcgccg taaaccagat attaagtaca
    43081 ataaagctaa tcaagacttt gaagccctcc aagagattca attgtctatc ctatctagcg
    43141 tttgtcaaac gctacgtaaa ggtggtataa taacctatag cacttgtacc atttttgagg
    43201 aagaaaattt tcaagttatt gaaaaattct tagaaaacca ccccaatttt gagcaagtag
    43261 aactgagtca tacacaagag gatattgtaa agagaggctg tattagtata agtcctgaac
    43321 aatatcatac agatggcttt tttattggac aagtcaaacg tatcttgtag tgtctaggag
    43381 gaaactgaca tcgtcagaaa aaaatatatg gaaatatctc ttttaacaga tattgggcaa
    43441 cgtcgttcca ataatcaaga ttttattaat caatttgaaa ataaagccgg cgtcccacta
    43501 atcatcttag cagatggtat gggaggacat cgtgcaggaa atattgctag cgagatgact
    43561 gtaactgatc taggaagtga ttgggcggag acagattttt ctgagttatc agaaattcgt
    43621 gattggatgc ttgtctctat tgaaactgag aatcgcaaaa tatatgaatt aggtcagtca
    43681 gatgattaca aaggtatggg aactaccatt gaagcagttg ccattgtagg agataatatt
    43741 attttcgcac atgtaggaga ttcacgtatc ggtatcgttc gtcaagggga gtatcattta
    43801 ttgacgagtg atcattctct tgttaatgaa ttggtaaaag ccggccaatt aacagaagaa
    43861 gaagctgcta gtcatcctca aaaaaatatt attacgcaat caattggtca agccaatcca
    43921 gtcgaacctg atttaggagt tcatctactt gaagaaggtg actaccttgt agttaatagt
    43981 gatggactaa ctaatatgtt gagtaatgcc gatattgcta cagtactaac acaagaaaaa
    44041 accttagatg ataagaatca agatttaatc actctagcaa accatagagg aggtcttgat
    44101 aatatcactg tggcactagt ttacgttgaa agtgaggcgg tttaatgatt cagattggca
    44161 aattatttgc tggtcgttat cggatcctta aatcaatcgg acgaggtgga atggcggatg
    44221 tttatctagc tcgagatcta attttagata atgaagaggt cgcaataaaa gtattgcgaa
    44281 ccaattatca aacagatcag attgcggttg ctcgcttcca gagagaagca cgagcaatgg
    44341 ctgagctgac acatcctaat attgtcgcta ttcgtgatat aggtgaagaa gacggacagc
    44401 agttcttagt aatggaatat gttgatggat tcgatttaaa aaagtatatt caagacaatg
    44461 cacccctatc aaataatgaa gttgttcgaa tcatgaatga agtcctttca gccatgtcac
    44521 tggcacatca gaaaggtatc gtacaccgtg atttaaaacc tcaaaatatt ttattaacta
    44581 aaaaagggac tgtaaaagtt acagatttcg gtattgcggt agcgtttgct gaaactagtt
    44641 taacgcaaac gaattctatg ttaggtagtg ttcattattt atcgccagag caggcgcgtg
    44701 gttcaaaagc gactgttcag agtgatattt atgccatggg aatcatgctt tttgaaatgt
    44761 taactggcca tattccttat gatggggata gtgcagttac tattgcccta cagcacttcc
    44821 aaaaaccact accatcaatc cttgcagaaa ataaaagcgt tccacaagct ttggaaaaca
    44881 ttgtcattaa agcaacagct aaaaagctaa cagatcgcta taagacaact tatgagatgg
    44941 gacgtgattt atcaactgct ctaagctcga cacgtcaccg tgaaccaaaa cttgttttta
    45001 atgatactga gagtactaag acactaccaa aagtaacatc tacagtatca tctttaacga
    45061 cagaacaact gttgcgtaat caaaaacagg ccaaaacaac agaaaaaata actcctgatt
    45121 ctgcgagtaa cgataaaacg aagtctaaaa agaaggcttc tcatcgactt cttggtacaa
    45181 tcatgaaatt attctttgca ctctgtgttg taggaataat tgtatttgct tataaaattt
    45241 tagtaagtcc tacgactatt cgtgttccag atgttagcaa caaaacagta gctcaagcaa
    45301 aaatgacatt ggaaaattct ggattgaaag taggagctat tcgtaatatt gagagtgata
    45361 gtgtttcaga aggccttgtc gtaaaaacag accctgctgc aggacgttct cgtagagaag
    45421 gggctaaagt taatctttat attgccactc caaacaagag tttcactcta ggtaattata
    45481 aggaacataa ttacaaagat atcctcaaag atcttcaagg caaaggtgtt aaaaaatctt
    45541 taatcaaggt taagcgtaaa attaataatg attatactac tggtacaatc ttagcacagt
    45601 cgcttccaga aggaacatct tttaatcctg atggtaataa gaaattaaca cttacggttg
    45661 ctgttaatga tccaatgatt atgcctgatg taacaggtat gacagttgga gaagtaatag
    45721 aaaccttgac agacctcgga ttagatgcag ataatcttgt cttctatcaa atgcaaaatg
    45781 gcgtttatca agcagtagta acacctccat cttcaagtaa gatagcaagt caggatcctt
    45841 attacggagg agaagttgga ttaaggagag gagataaagt taagctgtat cttcttggtt
    45901 ctaaaacaac aaataactca tcatctacgc caatagattc ttcggcttca agctcaacag
    45961 gaacaactac ctcggattct gtaagctcat caacggatgc tagcacttct gatagtagta
    46021 gcacttctac aagtagttca acactaccaa gtgatagtac aactaataca ggaacagcta
    46081 acaatccatt aactcaataa aattaaaaag aagatgttaa agtcttcttt ttaatttgct
    46141 atggtagaga ccatgcctag aaaggttctg ccaatatgcc aaatttttct ctcaataatg
    46201 gtgcaaagta tagggctttt tgataaaata cgtcatatag agtaatggaa ggattattat
    46261 gaaaaaattt cagttttttc tacttgttga agccgttgtc ttagttatgg gattaatgaa
    46321 aattttatca gatgactgga caagttttat ttttattctc gctttgattc ttttagcctt
    46381 aagattttat aacaacgata gtcgtcataa ttttctgtta acgacaagtt tgttattact
    46441 atttttaatc tttatgctta acccttacat tattgcagcc gttgtttttg cagttttgta
    46501 tgttctaatc aatcatttct cgcaagttaa gaaaaaaaat cgttatgctt tgattcaatt
    46561 taagaaccat caattagatg ttaagacaac acgaaaccaa tggttaggta ctgatcagca
    46621 cgaaagtgac ttttatgctt ttgaagatat taatattata agaatctctg ggactgatac
    46681 aattgattta acaaatgtta ttgtatctgg gcaggataat gttattataa ttcaaaaagt
    46741 ttttggtgat accaaagtat tggttccttt agacgttgcc gtaaaggctg atattagttc
    46801 tgtttacggt agtgtacagt actttgattt tgaggagtat gatctaagaa atgaatctat
    46861 aaaattgtca caagaggaag aatattacct tttgaaacgt gtcaaattag ttgtaaacac
    46921 tatcgctggg aaagttgagg ttagtcgtaa atgaaaaagc accactactt tttagctttc
    46981 ttctatggaa gtgttattat atttgccatt tgttttgtca ttattgatag tttgggtgtt
    47041 aatttggtgc acctctatca aacgtctcgt ttgtggctga ttgaacaact gattttctct
    47101 attttctttc tatctttagc ggtaactatc cttttattgc taacttggtt tttattagac
    47161 gataatagta aacgtcaaat taaccataat ttacgtcgca ttttaaataa ccaatcaata
    47221 aatgttaccg atgatggtac tgaaatatcg accaatattc aacggctctc taaaaaaatg
    47281 aatttaatga cagcaagtct acaatctaag gaaaatagtc gtattttaaa aagccaagaa
    47341 attgtgaaac aagaacgtaa gcgtattgca cgtgatttac acgacacagt tagtcaggat
    47401 ttgttcgcag cttccatggt tttgtcaggg attgcacaga atgtttctca gctagacgtt
    47461 gatcaggttg gctcgcaatt attagcagta gaggaaatgc ttcagcatgc acaaaatgat
    47521 ttgagaattc tgctacttca tttgaggcca gttgaattag aaaataagac cctatctgaa
    47581 ggattcaaaa tgatattgaa agaattgacc gataagagcg acattgaagt tgtttatcac
    47641 gaatcaattc taaccttacc caaaaagatt gaagacaata tttttagaat tggtcaagaa
    47701 tttatcagta atactttaaa gcattcacaa gccagtcgtc tcgaagtgta cctcaatcaa
    47761 acagagaatg aattgcagtt aaaaatgatt gataatggta ttggatttga tatggattct
    47821 gtctatgatt taagttatgg attaaaaaat attgaagatc gagtagaaga tttagcagga
    47881 aatttacagc ttctcagtca gcctggtaaa ggagttgcta tggatataag attaccttta
    47941 gtaaatcaga gtgaggataa aaatggataa aataaaaatt gtacttgttg atgaccatga
    48001 gatggttcgt ctcggtttaa aaagtttttt aaacttacaa gctgatgttg aagtaatagg
    48061 tgaagcctca aatggtttag aaggcataaa aaaagcactg gagttacgtc cagacgttgt
    48121 tgttatggat ttagtaatgc ctgaaatgga cggtgtagaa gcaaccttag cattgttgaa
    48181 ggattggcca gaagctgcta ttttagtctt aacatcttac ttggacaatg aaaaaattta
    48241 ccctgttatt gaagcaggtg ccaaaggcta tatgttaaag acctcaagtg cagcagagat
    48301 tcttaatgcc attcgtaaag ttgcaagggg agaacaagct attgaaaatg aagtagataa
    48361 gaaaattaag gctcatgata aatgtccagc tttacatgag ggcttaacag caagggaacg
    48421 tgatatcctt aatttattag ccaaaggtta tgacaatcaa aggattgctg atgaattatt
    48481 tatctcgtta aagactgtta aaactcatgt ttctaatatt ttgggaaaac taaatgtggc
    48541 agatcgaact caagcggttg tctatgcttt ccaacaccat ttggtaccac aagatgatga
    48601 ataagtgata taatagagac actatgttaa tgaaagtggt tagtcatgga cgcaaaaact
    48661 aaatataaag caaaaaaaat aaaggcggtg ttttttgata ttgatgacac gcttcgtgtt
    48721 aaagatacag gttatatgcc accatcgatc ttaaaagttt ttaaagcttt gaaagataaa
    48781 ggaattgttg taggtattgc ttcaggaagg gcacgatatg gcgttccaaa agaagttcaa
    48841 gatttaaatg ccgattattg tgtaaaatta aatggagctt atgttaagga caaggataag
    48901 aatatcatct tccatcgtcc tattcctgct gaatatgtag agcaatataa gaaatgggca
    48961 gacactgttg gtattaagta cggtttagct ggtcgtcatg aggcagtttt atcagatcgt
    49021 gatgatttgg taaatgatgc gattgatatt gtgtattctg atctcgaagt taatccagat
    49081 ttcaataaag aacatgatat ttatcagatg tggacgtttg aagataaagg agatagcctt
    49141 catctacctg aaccgttaga tgaacatctt cgcttgatac gttggcatga tcattcttca
    49201 gatgttgtgt taaaaggaac atcgaaggct ttaggtgttt ctaaagttgt tgaacatttg
    49261 ggcttaaagc cagagaatat cttggttttc ggagatgagc tcaatgattt agaactcttt
    49321 gattatgctg gactagctgt ggcgatggga gtgtcacacc cagaagctca aaaaaaagca
    49381 gattttatca caaaaaaagt agaagaagat ggcattttgt atgccctgga ggaactaggt
    49441 ttgatagaaa aagaattaac atttccacaa gtagatattg aaaatacaga aggtcctgtg
    49501 gctgtcatta aaacaaatca cggggatatg actgtaaaac tcttcccgga tcatgctcct
    49561 aaaacggtag caaactttat tggtttagct aaacaaggtt attatgatgg tattatcttc
    49621 catcgtatta ttccagattt tatgattcag ggtggtgatc caactggtac aggtatgggt
    49681 ggtgaatcta tctatgggga aagttttgaa gacgaatttt ccgaagaatt atataatgtt
    49741 cgcggagctc tttcaatggc taatgctgga ccaaatacga atggcagcca atttttcatc
    49801 gtccaaaata caaaaattcc ttatgcgaaa aaggaattag agcgaggtgg ttggccaaca
    49861 ccaattgctg agttgtatgc tggacaaggt ggtacaccgc atctagatcg tcgccacagt
    49921 gtatttggac aattagtaaa ccaaagttct tttgaggtac ttgatgagat tgcggcagta
    49981 gagactggtt ctcaagacaa accattggaa gatgttgtta ttttaacgat tgaggttaaa
    50041 gaataaaatg aagattggcg ataagttaaa gggaacagtc actggcatta ggccttatgg
    50101 agcctttgtt tctcttgagg atggtaggac tggacttatc catatttctg aaattaaaac
    50161 aggttacatt gataatattt atgatgttct tagtgtaggt gatgaagtct atgttcaggt
    50221 tatagatgta gatgaattta cacaaaaagc aagtttatcg cttcggaccc ttgaagaaga
    50281 aagacatcat attcagcatc gtcatcgatt ttctaacaat cgtcttaaga taggtttcaa
    50341 accacttgag gaaaatttac ctagttgggt tgaggaaggt ctagcctact tgaataataa
    50401 ataaacaaaa acctagattt gattaatgac aagtctaggt tttaaatatt taaaagtagc
    50461 agtgaatatt atacttgcta aaaatagctt gataatcgag tagatcctct gggtgtagtg
    50521 cagcgatctt ttccatttca tattgacgat taagtaatcg atatttgttt tgtccaaatt
    50581 ggtggaaagg taagagttgc acttgacgga tatctaggcg atcaaataaa caagcaaatt
    50641 cttcagcatc ttctaatgaa tcgttaaaat taggtatgac aggaatacgt agcacaatcg
    50701 ttttaccgtt ggcaaaagca taatgaatgt ttttgatgat tgaagcattc ttaaccatcg
    50761 tcttttcttg gtgtttgagg ctattgtaat gttttaaatc ggtgtaaata aaatcaacgt
    50821 attggataag gtctatgaat tgttcatgtc tagtataagc agttgtttca atagccgtgt
    50881 ggattcccag tgatttagca cgttttaaaa ttgccttggc aaattcaaat tgtgcaaata
    50941 tttcaccgcc agatagcgtt atgcccccac cagattcttc gtaaaaatca atatctttta
    51001 aaacctcttc aataatatca tcaaccgatt tttcttcgcc cacgatgacg ctttcattag
    51061 taatggcatc tctcattgtt tctggaacca ttttttgcga ctcgggatta gcacaccaag
    51121 gacatctaag aggacaaccc tttaagaaaa cggttgttcg aatacctgga ccatcgtgaa
    51181 tactaaaatg ttgaatgttg aacacaatac ctttttcatt tgtcatatta ttttcctcaa
    51241 tttcatttta agtatacctt ttatacgtaa caaagtcaat tacgaatgaa agtttattga
    51301 acttgtttaa taaataaaaa atgataaaat agaaaggagg tgtttcatga atagattaga
    51361 aaatattatt agtttagtat cccagtacca aaaaatagat gttaatacac tctcggagtt
    51421 gttgcaagtt tctaaagtaa ccatacgtaa agatttagat aaactagaag gcaaagggct
    51481 tttgcataga gagcacggtt atgcagtttt aaacagtggg gatgatctta atgttaggtt
    51541 atcttttaac cataaaacta aaaaagaaat tgctgcctta gctgcaaata tggtgtctga
    51601 caatgacact atcttaatag aatcaggctc tacttgtgct cttttagcgg aaaatatctg
    51661 tcagacaaag cgaaatgtca ctattttgac caactcttgc tttattgcta actatttacg
    51721 agagtatgat tcatgccaaa ttgtcttact tggtggtgag tatcaatcaa gttcacaggt
    51781 tacggtaggt cctctcttga aaaagatgat ttcacttttt catgtctcgt tagcttttgt
    51841 tgggactgat ggttttgatc caaaactagg atttatgggt agagatttaa tgcgttctga
    51901 agttgcacaa gagatggcga atgctgccga tgaagtcatt attttaacag attctagtaa
    51961 gtttaatcag actgcattgg tggaacagct tccgttgtca actgtatcac aagttattac
    52021 agataaacat ccaaattcgg atattgctaa cttgttccaa gaagcagaga ttaccattca
    52081 atctttatta caagtaccat aaaagaggct atttttctta cattttgcac atttgtggcg
    52141 ataatagata aagtgtcatg tcaaattgca cagatgtaac ctttttgtta aactgaagca
    52201 aatgctgaaa ggttatgagt atgaaattag agcgtcaaaa attgttagct aaagtagctt
    52261 acctttacta tatggaaggg aaaagccaat ctgagattgc caatgaatta ggaatttatc
    52321 gtacaacaat aagtcgtatg ttagccaaag cgagagaaga aggtcttgtt agaatcgaaa
    52381 tttcagattt taatccagaa atttttcagt tagaatccta ttttaaaagt aagtatcacc
    52441 ttaaagatat tgagattgtt tcttcgcgaa aagatagcga tacttcagag atagagaagg
    52501 atttagcaca tgtagctgca gctatgattc gaaaaaaaat taaagagaat gacaaggtgg
    52561 ggattgcttg ggggaggaca cttagtaaag tagttgaagc aatgcgtcct catccggttt
    52621 ctcaagtttc ttttgttcct ttggccggag gtccaagtca tattaatgca cgctatcacg
    52681 ttaatactct ggtttacgaa atgtcaaggc gtttccaagg aagttgtact ttcattaatg
    52741 caactctagt tcaagaaaac gctaatttag cgaaaggaat attaacttca aaatattttg
    52801 aggggctaat ggataattgg gaaaaacttg atgtggcaat tgtgggcgtt ggtggtaaac
    52861 ctaaaagcaa tgaacaacag tggcttgatc ttttaaatca agaagatttt caatgcttgg
    52921 atgaagaagc agctgtaggt gaaattactt gtcgtttttt taatcattca ggtgattctg
    52981 tgaatcaaca tttagctaaa aggacaatag gaattacctt agaacaacta cagaaagttc
    53041 ctaatagaat agcggttgct catggcaatt ataaggctgc ggcactctta gccgttttaa
    53101 aaaaggggta tattaatcat ttagttactg atttttccac agccttaaat attttaagat
    53161 tagataaaga cacctttgtg gatactatgt atcaaaagag ctaggattta ttcctagctc
    53221 ttttaataat gtttctatta ccaagtgaat tgttatagaa taagcacaaa agtgcgctat
    53281 tatcataaag ggcacaaggt ctattgacga cgattaaggg acatgttaca tttgttttgc
    53341 gaaagaaaat tagaaccttt tgtaagaaag tttaaactta taaaaaatca ttaaagcgga
    53401 ggtgcttatg gaaatcatag tcgcagacca aattattatg gggctcattt tgaatgctgg
    53461 ggatgctaaa caacatatct atcaagcctt aaaacttgca aaagaaggaa attttgcaga
    53521 aagtaaaatt gaaatagaac tagcagatag tgcactccta gaagctcata atcttcaaac
    53581 acaattcctt gcacaagaag caggagggac aaggacagat atctcagcac tctttattca
    53641 ttctcaggat catttgatga caagtattac tgagattaac ttgattaaag aaattattga
    53701 tttacgccag gaattacata ttcaaaaata agtaaagggg atcaaatatg ataaaaattg
    53761 gtttattctg tgcagcaggt ttttcaacag gtatgttagt aaacaatatg aaaatcgcag
    53821 cggataaaga agggatagag gcacatattg aagcttattc acaaggaaaa attgcagatt
    53881 atgctaaaga tttagatgtt gcattacttg gtccacaagt tagctatact ctagataaat
    53941 caaaatcaat ctgtgatgaa tacggtgtac cgatagcagt tattccaatg gcagattatg
    54001 gtatgttgga tggtgtaaaa gttttgaaac ttgctttgag tttattggaa acaagaaaag
    54061 aggcttgata tgtcaaagtt tgatagtcag aaaataatta ctccgattat gaagtttgtc
    54121 aatatgcgag ggattattgc actcaaagat ggcatgctag caattttacc actaacagtt
    54181 gttgggagtc tctttttaat attagggcag cttccattta aaggtcttaa tcaagccata
    54241 gctaatgttt ttggaccaga atggacagaa ccatttatgc aagtttattc aggaactttt
    54301 gcgattatgg gcttgatttc ctgttttgca attgcttatg cttatgctaa aaatagtggt
    54361 gtagaaccgt tacctgctgg cgttttatca ctttcgtctt tctttatttt aatgaaatca
    54421 tcttatattc ctgtaaaagg agaagcaata gcggacgcta tttctaaggt ttggtttggc
    54481 ggtcagggga ttattggagc aattattatt ggcttagtag ttggtgctat ctatacatgg
    54541 tttatccaac atcatattgt tataaaaatg ccagagcaag taccacaggc aatagcaaaa
    54601 caatttgaag ctatgattcc agcttttgtt atctttcttt tatcgatgat tgtttatttg
    54661 attgctaagg taacaactgg aggtaccttt attgagatga tttatgatat cattcaagta
    54721 ccgttgcaag gcttaacagg ctcactttat ggagcaattg ggattgcttt ctttatttca
    54781 ttcttatggt ggtttggtgt ccatggccaa tctgtggtga atggtattgt gactgctttg
    54841 ttactatcca atttagatgc caataagtct ttattagcag caaatcgctt aacattagat
    54901 aatggcgctc acattgtaac acagcagttt ttggatagtt tcttgatttt atcgggctca
    54961 ggaataactt ttggactagt tattgcgatg ctttttgcag caaaatcaaa acaatacaag
    55021 gcacttggga aagtggccgc ttttcccgca attttcaatg ttaacgagcc aatcgtcttt
    55081 ggctttccaa tcgtaatgaa tcctgtgatg tttctgcctt ttattttagt accagttttg
    55141 gcagctttaa ttgtttatgg agctattgcg gttggtttta tgcaaccatt ctcaggtgtt
    55201 actttaccat ggagtactcc agcaattatt tctggattca tggtaggtgg ttggcaaggt
    55261 gcactcgtcc aaatagtcat tttagctatc tcaactgctg tttacttccc attctttaaa
    55321 atccaagata atattactta caaaaatgaa tgtgaaatgg aaaggggata gctagcagat
    55381 aagaggttga ggtgtgtaat gcaccttaac ctttttctaa aaatgcacat ttgtgcaact
    55441 ttagatgtag cattctattc tttattttct tcttgttatt ttaggattta ggtgctatta
    55501 tatttacgaa gaaaaacttt ttagtttcga aagaaacaag cggaggctgt tatgaccaaa
    55561 caattactca ctcaaaaata tacaaatgat gttcaacaaa attctcaaaa gcattttggc
    55621 tatttgacag agcgcatgta ttcttatcgc gataaggttc ttgataagaa accattcata
    55681 gatgcagaac gtgctatctt ggtaactgaa gcctatcaaa agcatcagga aaaacctaat
    55741 gttttaaaga gagcttacat gttgcaaaat attttggaaa aaatgacaat ctatattgac
    55801 gatgagacaa tgattgtcgg taaccaagct tcatctgata aagatgctcc tatttttcca
    55861 gaatacactc tagaatttgt tgtaaatgaa ctagaccttt ttgaaaaacg agatggtgac
    55921 gtcttttaca ttacagaaga aaccaaagaa caaattagaa atattgcacc tttctgggaa
    55981 aataataatt tacgggctcg tgcgggcgtt atgcttccag aagaagttca agtttatatg
    56041 gagacaggct tctttgggat ggaaggtaag atgaattctg gggatgctca cttagctgtt
    56101 aactatcaga agttactaga agaagggctt attggctttg aaaaaaaagc aagaaaagct
    56161 aaagctgact tagatttaac aaagcctgag agtattgaca aatatcattt ctatgattct
    56221 attttaatta caatagaagc tgttaaaacc tatgcagagc gttttgctat cctagcaaaa
    56281 aaacaagcta aaacagcaaa tactaagaga cgtcaggaat tgttagatat cgctagtatt
    56341 tgtgaacgag tgccatatta cccagctgaa acgtttgcag aagctgttca atcggtttgg
    56401 tttattcagt gtattcttca aatagaatca aacggtcatt ctctatctta tggacgcttt
    56461 gatcaataca tgtatccata tgttaaagct gacttagaag ctggacgtga gactgaggat
    56521 tcgatcgttg agcgcctaac taacctatgg attaaaacaa taactattaa taaggttcgc
    56581 agccaagctc atacgttttc gtctgctggt agtcctcttt accaaaatgt aacgattgga
    56641 ggacaaaccc gacataagga agatgctgtt aatcctttat catttctagt actaaaatca
    56701 gtcgctcaga cacacttacc acagcctaat ctaacggttc gttatcatgc taacctagac
    56761 aagtcattca tgaacgaagc tattgaagtt atgaaattag gttttggtat gccagctttt
    56821 aataatgatg aaattattat tccatcattt attaaaaaag gagtttccga agaagatgcc
    56881 tatgattatt cagcgattgg ttgtgttgaa acagcagttc ctgggaaatg gggttatcgt
    56941 tgtaccggca tgagctatat caacttccct aaagtgttat taattacaat gaataatggc
    57001 attgacccgg cttctgggaa gagatttgcg ccttcttatg ggcatttcac ccagatgacc
    57061 tcatataaag aacttaaaga agcttgggac aaaacacttc gttacttaac tcgaatgagt
    57121 gttattgttg aaaatgctat tgatattagt ctcgaaagag aagtacctga tattctttgt
    57181 tcagctttaa ctgatgattg tattggacga ggtaagcatt taaaagaagg tggtgcagta
    57241 tatgactaca tatctggtct acaagttgga atcgctaatc tttcagactc acttgctgcg
    57301 cttaaaaaat tagtatttga agaaaaacgc ctaaccacat tagaagtatg gcaagcattg
    57361 cagagtgatt acgcaggtcc gcgtggtgaa gaaattcgtc aaatgttgat caatgaagca
    57421 ccaaaatatg gaaatgatga tgactacgcg gatagtttag tacgtgagtg ttatgatgtt
    57481 tatgttgaag aaattgctaa atatcccaac acacgctatg gacgtggacc aatcggtggt
    57541 attcgctatt caggaacgtc ctctatttca gcaaatgtag gtcaaggacg aggtacttta
    57601 gcaactccag atggtagaca tgcaggaact ccattagctg aaggttgttc accatctcat
    57661 aatatggaca aaaagggacc tacctctgtt ttgaaatcag tttcgaagtt accaactgat
    57721 gagattgtcg gcggtgtact ccttaaccaa aaagtcaacc ctcaaacctt ggcaaaagaa
    57781 gaagataaac aaaaattaat tgccttactt agaacattct tcaatcgcct tcatggttac
    57841 catatccagt ataatgttgt ctcacgtgag actttgattg atgctcagaa gcaccctgaa
    57901 aaacaccgcg atttgattgt gcgtgtagca ggttattctg cattctttaa tgtcctatct
    57961 aaggcaactc aagatgatat tattgcacgt acagaacatg ctctttagga gattattatg
    58021 gaatttttac ttgacacttt aaatttagaa gctataaaaa aatggcatca tatactgcca
    58081 ctagcaggtg taacttcaaa tcccaccatt gctaagaaag aaggagatat acattttttc
    58141 caacgcattc gtgatgttcg agaaattatt ggatgtgaag caagtttgca tgttcaagta
    58201 gtggccaaag attatcaagg gatactggat gatgctgcta aaatacgcca agaaacggat
    58261 gatgacattt atatcaaggt tccagttacg ccagatggat tagcagcaat taaaacgtta
    58321 aaagcagaag gttataatat tacagccaca gcaatataca ccagtatgca aggtcttttg
    58381 gctatatcag ctggagcaga ctacctagca ccttacttta accgaatgga gaaccttgat
    58441 attgatgcga cacaagttat taaggaatta gcgcaagcaa tagagaggac aggttcttct
    58501 agcaaaattt tagcagctag ttttaaaaac gcttcacaag taacgaaggc cttatcacag
    58561 ggtgctcaat caatcaccgc gggaccagat atttttgaat cagtctttgc gatgccatct
    58621 attgcaaaag ctgttaacga ctttgcagat gattggaaag ccagtcaaca ctcagaacat
    58681 atttaacaga gaacgccaac ttttgaggct gtatctttaa cataaaattt tttaaagaag
    58741 aaagaggttg agaatgaaag ttttcgcaag tccatcgcgc tatatacaag gaaaggatgc
    58801 tttattccaa agtattgaac atatcaaatc tttaggtcag actccactta tcctttgtga
    58861 tgatgttgtt tataatatcg ttggagaaag gtttttatct tatctacaag acgatttgtt
    58921 accacatcgt gtctccttta acggagaagc atctgataat gaaatcaatc gtgtcgttgc
    58981 tgttgcaaaa gaaaagaata gtgaccttat tattggactt gggggtggaa aaactattga
    59041 ctctgcaaag gcaattgcag ataaagttaa tttaccagtg atcattgcac caacagtagc
    59101 ttcaacagat gccccaacat cagcgttatc agtaatctat acagatgaag gagcttttga
    59161 aaaatatatt ttttattcaa agaatccaga cttagttttg gtcgatactc aagtcattgc
    59221 tcaagcgcca aaacgtttac tagcttctgg gattgcagat ggcttagcta cttgggttga
    59281 agcgagagca gtcttacaaa aaaatgggat agcgatggct ggcggtagac agactttagc
    59341 gggcgtggca attgctcaag catgtgagcg tactttattt aatgatagtt tacaagcttt
    59401 agcggcttgt gatgtcaaag tggttacaaa agctttagaa aatgttattg aagctaatac
    59461 cttacttagt ggtttaggtt ttgaaagcgc aggattagct gcagctcatg ctattcataa
    59521 tggttttact gccttgagtg gagatattca tcacttaacc catggtgaaa aagttgccta
    59581 tggaacgttg acacaacttt tcctcgaaaa tagaccaaag gaagaaattg atcgctacat
    59641 taatctttac caagcgattg gaatgccaac tacacttgca gaactccatt tgggtgatgc
    59701 aacatatgaa gaacttttaa aagttggtca gcaagcaaca attgaaggtg aaacaataca
    59761 tgaaatgcca ttcaaaatta gtgcagagga tgtcgctgca gccctgttag cagttgaccg
    59821 ctatgtttca aatcatcaat aaaaacaata gaaccaagtg aaattgcttg gttctatttg
    59881 tcgttttaat attttaacta aatagggctt actagtattt aaagagatat aaactaaaaa
    59941 agatcagaca acatatctaa tcttttttag ttatcgttta ctgaagatta aagttattca
    60001 aaatcataca ggcttgttga taagtaacgt tcgccattat cagggagaag agctaaaacc
    60061 tttttccctt ttcctagttg tttggccact tcaatagcag cataaatagc agctgaagca
    60121 gaaattcctg cgaggaaacc ttctagtccg ccgatgatac gtcctgtttc aatagcatcg
    60181 ttagatgtaa cgcgaatgat atgatcataa gaatctgtag ccaatgtttc tggaatgaaa
    60241 cctgcagaaa tcccttgtat tttatgaggc ccaggttgct caccagaaag gattgcagat
    60301 tcatcagctt ctacagcata aatagcgaca tcaggattga ctttttttaa tgcacgagag
    60361 acaccagtaa ttgtcccacc agttcccaca cctgcgataa aagcatctaa acctttttca
    60421 ccaaaagtct cgataatttc cctaccagtc gttttttcgt ggatttctgg attggcttga
    60481 ttattaaatt gcaggggtag ccaagcattt tgctctgcag aaatttcttt tgccttagca
    60541 atagctccct tcatcccttc actaccaggt gttagtacta attgagcacc ataggcttga
    60601 atgattttac gacgttcaat tgacatggtt tctggcataa caataataac attataccct
    60661 ttagcttttc ctacccaagc caaaccgata cctgtattac cagatgtagg ttctacgatt
    60721 gtatctccgg ctttaagaat ccctttttgc tctgcatctt caatcatagc tagagcgata
    60781 cgatctttga cagatgaacc tgggttaaag gattctaact taacgtacac ctctgcagct
    60841 ccttctggaa caatatgatg taattgaata atgggtgtat ttccgattaa gtctgtaata
    60901 gaattataaa tttttgacat aagctatacc tcttcatgac attggttaag aatattataa
    60961 aactcacatt gattaaagta aaatatcaat tctttatcaa gtgatagcta ttatttatcg
    61021 gtttcagcct taaagggaat ttcaacaatt tgactccctt cttcagttaa caaagctttt
    61081 ccttggtaga actctgtcaa attagttatt gtgtgttcaa attccttagg gtcaacgtat
    61141 attttacagg ttacagcttc taaaaattct gtttcaaact cctgaaggtg gtgctctttt
    61201 aggaagttgt caaaagtttg atattgagga taagttaatt gaatacgaat accaatttgt
    61261 tctttaactt ctactacccc gatctctttg atggcattag ctacgctacc agagtaggca
    61321 cgaataagcc cccctgtgcc taatttgata cctccaaaat accgtgtaac gacggcaaca
    61381 acatttgtca ggccttgctt ttcaagtacg gttagcatag ggatacctgc tgtaccacta
    61441 ggttcaccat catcactaga tcgttttata tccccttttt cgccaataac catagctgaa
    61501 cacgaatgat tggctttgta gtgcgctttc ttgatttgcg taatataatt acgcccctcc
    61561 tcttcggact ctactcgctt tagatgacaa ataaatcgcg attttttaat ttcttcttcg
    61621 acaattccgt ctgattttat cgttttatag ttcatagaga aattttacaa aaattcttca
    61681 aatttagcaa aaaaatccga atgaaataat atggaaaatt atttaggtag actttggaca
    61741 aaagcacaat tatctgagca acttagaaaa attgcaatta acttgccatc ttttataaag
    61801 aaaggaagcg actatatttg tactcgatgc tctagttctg ttgccaaaaa ttgccaatta
    61861 ccaactggaa attattattg tagagaatgt atagtttttg gaagagttac tagtaatgaa
    61921 aatttatatt atttcccaca aaaaacattt tctaaaacga attctttaaa atggaaaggg
    61981 ggactgacac cttatcaaaa tgaggtttca gaagagttgt tgaaaggtat ttcaagtaag
    62041 gaaaatcttc ttgttcatgc tgtgacaggg gctggtaaaa cagaaatgat ataccactct
    62101 atcgccaaag taattgatac tggagggagc gtttgtattg ccagtcctag aattgatgtt
    62161 tgtttagaat tgcataagcg actttcaaac gattttagat gtgccataac attaatgcat
    62221 ggagaatctc cttcttatca gagatcccct ttaactattg ccacaacaca tcaattgtta
    62281 aaattttatc atgcttttga tttgctaatt gttgatgaag ttgatgcttt tccatatgtc
    62341 gacaacccga tactatacca tggtgtaaaa caggccttaa agaaaaacgg aacaagtatt
    62401 tttctgactg caacttcaac gactgagtta gaaagaaaag ttgcaagaaa agaactaaaa
    62461 aaattacatc tagctaggag atttcatgcc aatccgttag tgatacccga gatggtttgg
    62521 gtaagtggta tccagaaaag cttgcagaca caaaaactac ctcccaaatt atatcagctg
    62581 ataaataaac agcgacaaac gagatatcct ttgttattat ttttccccca tattgcagaa
    62641 ggtcaggtat tcaccgaaat gttaagacaa gcctttccta tggaaaaaat tggttttgtt
    62701 tcaagtaaga gcacatctag gttaaagtta gtgcaagatt ttcgagataa caaattgtct
    62761 atcctagtat caactaccat tctagagaga ggagtaactt tccctagcgt cgacgttttt
    62821 gttatacaag caaatcatca tttgtttacg aaaagttcct tggtacaaat ttcaggaaga
    62881 gttggaagag cattagaaag acccgaaggg ctcctttact tccttcatga tgggaaaagt
    62941 aaatcgatgc accaagccat aaaggagatt aaaaacatga atcatatagg aggattttaa
    63001 tgacttgttt gttatgtcat gaaatagatt tatcgcaatt aacttttgtg gaacttatac
    63061 ttctaaaacc aaagcaaaac gtaatttgtc aaacgtgcaa gggtagtttt gaagcactta
    63121 gtagggagat gggttgccaa acatgttgca agcaaatcct ccgaaaacaa tgtcaagatt
    63181 gtatttattg gggtaaaaaa ggtatagagg taaatcattt ttccctttat agatacaatg
    63241 aagcaatgaa aaaaatattt ttctcttttt aaatttcaag gggattattt gttgaaagat
    63301 gtttttacaa aagaaataaa ggctgctcta aaaaggtata agggctacac tatagtgcca
    63361 gttcccttga gtcatgaagg ataccaaaat aggcaattta atcaagtgat tgcttttcta
    63421 caatcggcaa atatacctta taaaaatatt ctttctaaaa aagatggagg taagcaatcg
    63481 gctaataata aagaagaaag actcaaacaa gttcagcaat ttacattaaa aaatgaggct
    63541 gagttaaggg ataacctttt aattgttgat gatatttata caacaggcgc aacaatagca
    63601 caaatcagga aactattaga agaaaaaggt ataaaaaata taaaaagttt ttcattagca
    63661 cgctaaaatc ttgcaaattt tcatgaaagc ggttataata agaatataga aagcgttaac
    63721 aacgcaagaa agaggtactc atatgattaa atacagtatt cgtggtgaaa acatcgaagt
    63781 aacagaagct attcgtgagt atgttgagac caaactctct aaagttgaaa aatactttaa
    63841 tgaagaacaa gaattagata ctagagtcaa cctcaaagta tacagagaga agaccgcaaa
    63901 agttgaagta acaatcttaa tagattctat tactttacga gcagaagatg tttcacaaga
    63961 tatgtatggg tcaattgact tagttgttga taaaattgag cgtcaaattc gtaaaaataa
    64021 aactaaaatt gcgaaaaaat atcgtgaaaa aattccagca agtcaagttt tcacaacaga
    64081 atttgaggct gagccagatg aagaagcagt aagccaaaga attgtacgta ctaagaatgt
    64141 taatctaaaa ccaatggatg ttgaggaagc attgttgcaa atggaacttt taggtcatga
    64201 tttctttatc tatacagatg cagaagataa tacgacaaat gtcctttaca agcgtgaaga
    64261 tggcgaacta ggtttaattg aagctaaagg aaattaactt tgtaacataa tttaaaaaat
    64321 ctcagagttt tttctgagat ttttttaaaa cctattgaca acaaataaaa aattatagta
    64381 tactaaatga gttcttaagc gaacctaata ttgtattatc attttataaa caacttaaaa
    64441 aaagtagttg acaaagcaga ataagtttgc tagaatatag aagt
//
LOCUS       DATROY010000002        59027 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.002, whole genome shotgun sequence.
ACCESSION   DATROY010000002 DATROY010000000
VERSION     DATROY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59027
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            42..524
                     /locus_tag="VBD11_000062"
     CDS             42..524
                     /locus_tag="VBD11_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953044.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(595..1704)
                     /gene="dinB"
                     /locus_tag="VBD11_000063"
     CDS             complement(595..1704)
                     /gene="dinB"
                     /locus_tag="VBD11_000063"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6953045.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            1874..4186
                     /gene="pflB"
                     /locus_tag="VBD11_000064"
     CDS             1874..4186
                     /gene="pflB"
                     /locus_tag="VBD11_000064"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6953046.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            4287..4660
                     /locus_tag="VBD11_000065"
                     /pseudo
     CDS             4287..4660
                     /locus_tag="VBD11_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(4695..5624)
                     /locus_tag="VBD11_000066"
     CDS             complement(4695..5624)
                     /locus_tag="VBD11_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6953047.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(5621..6376)
                     /locus_tag="VBD11_000067"
     CDS             complement(5621..6376)
                     /locus_tag="VBD11_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6953048.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            6501..7397
                     /locus_tag="VBD11_000068"
     CDS             6501..7397
                     /locus_tag="VBD11_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6953049.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(7476..8324)
                     /locus_tag="VBD11_000069"
     CDS             complement(7476..8324)
                     /locus_tag="VBD11_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6953050.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGSTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(8492..8944)
                     /locus_tag="VBD11_000070"
     CDS             complement(8492..8944)
                     /locus_tag="VBD11_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6953051.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(8962..10164)
                     /locus_tag="VBD11_000071"
     CDS             complement(8962..10164)
                     /locus_tag="VBD11_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6953052.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            10320..10979
                     /locus_tag="VBD11_000072"
     CDS             10320..10979
                     /locus_tag="VBD11_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6953053.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            11003..13288
                     /locus_tag="VBD11_000073"
     CDS             11003..13288
                     /locus_tag="VBD11_000073"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6953054.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            13292..13651
                     /locus_tag="VBD11_000074"
     CDS             13292..13651
                     /locus_tag="VBD11_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953055.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            13697..14677
                     /locus_tag="VBD11_000075"
     CDS             13697..14677
                     /locus_tag="VBD11_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6953056.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(14762..16510)
                     /gene="cydC"
                     /locus_tag="VBD11_000076"
     CDS             complement(14762..16510)
                     /gene="cydC"
                     /locus_tag="VBD11_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6953057.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(16503..18221)
                     /gene="cydD"
                     /locus_tag="VBD11_000077"
     CDS             complement(16503..18221)
                     /gene="cydD"
                     /locus_tag="VBD11_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6953058.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(18221..19240)
                     /gene="cydB"
                     /locus_tag="VBD11_000078"
     CDS             complement(18221..19240)
                     /gene="cydB"
                     /locus_tag="VBD11_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6953059.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(19241..20668)
                     /locus_tag="VBD11_000079"
     CDS             complement(19241..20668)
                     /locus_tag="VBD11_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6953060.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(20771..21979)
                     /locus_tag="VBD11_000080"
     CDS             complement(20771..21979)
                     /locus_tag="VBD11_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6953061.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(21992..22891)
                     /locus_tag="VBD11_000081"
     CDS             complement(21992..22891)
                     /locus_tag="VBD11_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6953062.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(23300..23905)
                     /locus_tag="VBD11_000082"
     CDS             complement(23300..23905)
                     /locus_tag="VBD11_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953063.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            24788..25087
                     /locus_tag="VBD11_000083"
     CDS             24788..25087
                     /locus_tag="VBD11_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6953064.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            25247..26437
                     /locus_tag="VBD11_000084"
     CDS             25247..26437
                     /locus_tag="VBD11_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6953065.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(26507..27232)
                     /locus_tag="VBD11_000085"
                     /pseudo
     CDS             complement(26507..27232)
                     /locus_tag="VBD11_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(27359..27946)
                     /locus_tag="VBD11_000086"
     CDS             complement(27359..27946)
                     /locus_tag="VBD11_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6953066.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(27990..28526)
                     /locus_tag="VBD11_000087"
     CDS             complement(27990..28526)
                     /locus_tag="VBD11_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6953067.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            28661..29833
                     /locus_tag="VBD11_000088"
     CDS             28661..29833
                     /locus_tag="VBD11_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6953068.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(29877..30659)
                     /locus_tag="VBD11_000089"
     CDS             complement(29877..30659)
                     /locus_tag="VBD11_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6953069.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            30818..31087
                     /gene="rpsN"
                     /locus_tag="VBD11_000090"
     CDS             30818..31087
                     /gene="rpsN"
                     /locus_tag="VBD11_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6953070.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
     gene            complement(31448..32473)
                     /locus_tag="VBD11_000091"
     CDS             complement(31448..32473)
                     /locus_tag="VBD11_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6953071.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(32593..33603)
                     /gene="tsaD"
                     /locus_tag="VBD11_000092"
     CDS             complement(32593..33603)
                     /gene="tsaD"
                     /locus_tag="VBD11_000092"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6953072.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            complement(33679..34086)
                     /gene="rimI"
                     /locus_tag="VBD11_000093"
     CDS             complement(33679..34086)
                     /gene="rimI"
                     /locus_tag="VBD11_000093"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6953073.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            complement(34088..34780)
                     /gene="tsaB"
                     /locus_tag="VBD11_000094"
     CDS             complement(34088..34780)
                     /gene="tsaB"
                     /locus_tag="VBD11_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6953074.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVSEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            34959..35189
                     /locus_tag="VBD11_000095"
     CDS             34959..35189
                     /locus_tag="VBD11_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6953075.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            35243..36922
                     /locus_tag="VBD11_000096"
     CDS             35243..36922
                     /locus_tag="VBD11_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6953076.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(37080..37589)
                     /locus_tag="VBD11_000097"
     CDS             complement(37080..37589)
                     /locus_tag="VBD11_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6953077.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(37737..39083)
                     /gene="glnA"
                     /locus_tag="VBD11_000098"
     CDS             complement(37737..39083)
                     /gene="glnA"
                     /locus_tag="VBD11_000098"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6953078.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(39117..39488)
                     /locus_tag="VBD11_000099"
     CDS             complement(39117..39488)
                     /locus_tag="VBD11_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6953079.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(39568..40107)
                     /locus_tag="VBD11_000100"
     CDS             complement(39568..40107)
                     /locus_tag="VBD11_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6953080.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            complement(40370..41566)
                     /locus_tag="VBD11_000101"
     CDS             complement(40370..41566)
                     /locus_tag="VBD11_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6953081.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(41701..42570)
                     /locus_tag="VBD11_000102"
     CDS             complement(41701..42570)
                     /locus_tag="VBD11_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6953082.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            complement(42779..43789)
                     /gene="gap"
                     /locus_tag="VBD11_000103"
     CDS             complement(42779..43789)
                     /gene="gap"
                     /locus_tag="VBD11_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6953083.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            complement(43993..46071)
                     /gene="fusA"
                     /locus_tag="VBD11_000104"
     CDS             complement(43993..46071)
                     /gene="fusA"
                     /locus_tag="VBD11_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6953084.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            complement(46226..46696)
                     /gene="rpsG"
                     /locus_tag="VBD11_000105"
     CDS             complement(46226..46696)
                     /gene="rpsG"
                     /locus_tag="VBD11_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6953085.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(46718..47131)
                     /gene="rpsL"
                     /locus_tag="VBD11_000106"
     CDS             complement(46718..47131)
                     /gene="rpsL"
                     /locus_tag="VBD11_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6953086.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(47350..48162)
                     /gene="purR"
                     /locus_tag="VBD11_000107"
     CDS             complement(47350..48162)
                     /gene="purR"
                     /locus_tag="VBD11_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6953087.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            complement(48259..49200)
                     /locus_tag="VBD11_000108"
     CDS             complement(48259..49200)
                     /locus_tag="VBD11_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6953088.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            complement(49190..50464)
                     /locus_tag="VBD11_000109"
     CDS             complement(49190..50464)
                     /locus_tag="VBD11_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6953089.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            complement(50466..51098)
                     /locus_tag="VBD11_000110"
     CDS             complement(50466..51098)
                     /locus_tag="VBD11_000110"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6953090.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            complement(51091..51753)
                     /locus_tag="VBD11_000111"
     CDS             complement(51091..51753)
                     /locus_tag="VBD11_000111"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6953091.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKYSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            complement(51760..52632)
                     /gene="rsgA"
                     /locus_tag="VBD11_000112"
     CDS             complement(51760..52632)
                     /gene="rsgA"
                     /locus_tag="VBD11_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6953092.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(52788..53639)
                     /locus_tag="VBD11_000113"
     CDS             complement(52788..53639)
                     /locus_tag="VBD11_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6953093.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            complement(53643..54515)
                     /gene="rsmA"
                     /locus_tag="VBD11_000114"
     CDS             complement(53643..54515)
                     /gene="rsmA"
                     /locus_tag="VBD11_000114"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6953094.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            complement(54595..55086)
                     /locus_tag="VBD11_000115"
     CDS             complement(54595..55086)
                     /locus_tag="VBD11_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953095.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            complement(55105..55665)
                     /gene="rnmV"
                     /locus_tag="VBD11_000116"
     CDS             complement(55105..55665)
                     /gene="rnmV"
                     /locus_tag="VBD11_000116"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6953096.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            complement(55652..56434)
                     /locus_tag="VBD11_000117"
     CDS             complement(55652..56434)
                     /locus_tag="VBD11_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6953097.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            complement(56616..56897)
                     /locus_tag="VBD11_000118"
     CDS             complement(56616..56897)
                     /locus_tag="VBD11_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953098.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            complement(56907..57299)
                     /locus_tag="VBD11_000119"
     CDS             complement(56907..57299)
                     /locus_tag="VBD11_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953099.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            complement(57290..58582)
                     /locus_tag="VBD11_000120"
     CDS             complement(57290..58582)
                     /locus_tag="VBD11_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6953100.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            complement(58582..58974)
                     /locus_tag="VBD11_000121"
     CDS             complement(58582..58974)
                     /locus_tag="VBD11_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6953101.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
BASE COUNT        18869 a        11723 c         9461 g        18974 t
ORIGIN      
        1 ttaatacttt tattaaggtt tatttagaaa aaggagatat tatggtttca tatgaaaaag
       61 tccgccgctc tctgcgcaca gctacaatca ctatcattgt attaaatagt ttgtctttag
      121 tattcagatt gtttacagga atttcagtgc agctagccaa aactgaaatt aataaaggga
      181 acacaggtaa tcttcctaaa gaacatatcg aagcagtctt gagcgccaca acaccattta
      241 tgctatttgt cactgcttta atagtattag tcaacattgc cattgttatt ttctgtatta
      301 aaaatttaag agcaattaaa agaaatcaaa tggttaatta ccttccttat taccttggct
      361 tcgccattac tgtaggactt gttattttgg gttttctaac tactaaagca ccatgggcaa
      421 tagctataaa tatcgtcttc caagctattt tcggtcttct ttatttccat gcttatcaaa
      481 aagcacaaaa actcaacgaa cgtgatcttg aagagactaa ctaaaagtaa aagcactaga
      541 atctagtgct tttttctttt caaagagagt taaatatagt catcgtccca acttttaaaa
      601 atctaaatag atagcttcat gttcattttc cagggtcgtt actgtaacac ctaagagtcg
      661 tatgccaaaa actgactctt ctaaggtatc aaatatcgct ttagcaactt gatcaatgat
      721 ttgaaaatct tgagtatact cttctaatgt catacgtttc gtcaatgttt caaaatccgc
      781 atatctaact tttaaaacga ttgtcttacc aactttctta tttttctcca gagaagcaac
      841 aactctctga acgttctttg aaatctcagc tttgatatct gcttcgttat atagtaattt
      901 cccataggtt ttctcacttc ctattgattt acgaactcta ttaggtttta ccggggaagc
      961 attaatccct cttgccttac gataaaggtc atacccgaaa cgaccaaaca tatcaattaa
     1021 agaaatttca gataaggaaa ggaggtcctc accagtataa acccctaaag catgtaattt
     1081 ttcgacggag cgtttaccaa caccatgaaa tttctctatt ggtaagggct tcaagaaatc
     1141 ttgagcttga tctggtagga tgaaggtaag tccttttggt ttctcaaagt cagaagccaa
     1201 ttttgccaaa aatttattgt aggaaatacc cgctgaacag gtcaaatgga catcattcca
     1261 aatatcgtat tgtatcattt tagcaagttt aacagctgat tttattccca ttttattttc
     1321 agttacatct aaataagctt cgtctatact catcggttca accaaatccg tatacttttt
     1381 aaatatatct cgtacttcca ttcctacttg gcgatatttt tggtaatttc ccgaaataaa
     1441 gatagcttga gggcatcgtt catacgcttc ttttgaagac atagctgaat gaactccaaa
     1501 ctttcgagcc tcataattac aagtagaaac aacccctctg ccgccggttt ttctgggatc
     1561 agaaccaata ataacaggct ttcctttaag agagggattg tcacgctctt ccacagatgc
     1621 aaaaaaagca tccatatcaa tgtggataat tttacgcgac gtatcattta ttaaaggaaa
     1681 aattaacatt gttctctccc ccattctctc tactccatta taacagatta caacttaatg
     1741 atacagatta aaaaaactgt attataaaag aatcataatt ttgtcaagaa atatgcttgc
     1801 gaaaacgttt actttttcct aaaatggata tgtaaacaaa attaaatctg tcacaatgtt
     1861 taaggagaaa actatggcaa cagttaaaac aaacacagat atctttgaac aagcttggga
     1921 aggctttaaa ggtgttgatt ggaaagaaaa agcaagtatt gctcgcttcg ttcaagcaaa
     1981 ctatgcacct tacgatggag atgaaagctt cttagctgga gcaactgaac gttcacttca
     2041 catcaaaaaa gtgattgaag aaacaaaagc tcactatgaa gaaactcgct tcccaatgga
     2101 tactcgtgtt gcttcaattt ctgaattacc agctggtttt atcgacaaag ataacgagct
     2161 tatctttggt attcaaaacg acgagctttt caaattgaat ttcatgccta aaggtggtat
     2221 ccgtatggcg gaaactacac ttaaagaaaa tggttatgaa cctgatccag ctgttcacga
     2281 aatcttcact aagtacgcta caacagtaaa tgatggtatc ttccgcgctt atacttctaa
     2341 cattcgtcgt gctcgccatg cacacactgt tactggtctt ccagacgctt attcacgcgg
     2401 acgtatcatt ggtgtatatg ctcgtctagc tgtttacggt gctgactact tgatgcaaga
     2461 aaaagttaat gactggaatg cattgaatga tattgatgaa gagtcaattc gtcttcgtga
     2521 agaaatcaac cttcaatacc aagctcttgg tgaagttgta aaacttggtg acctttacgg
     2581 cgtagatgtt cgtaaaccag ccatgaacac taaagaagcc attcaatggg tgaacattgc
     2641 tttcatggct gtatgtcgtg ttatcaatgg tgctgcaaca tcacttggac gtgttcctat
     2701 tgtccttgat atctttgcag aacgtgacct cgctcgcggt acattcacag aatcagaaat
     2761 ccaagaattt gttgatgatt ttgtccttaa actccgtact gtaaaatttg ctcgtactaa
     2821 agcttatgat gccctttact caggtgaccc aacctttatc acaacttcaa tggcaggtat
     2881 gggagctgat ggacgtcatc gtgttaccaa aatggactat cgtttcttaa atacacttga
     2941 caatatcggt aactcaccag aaccaaactt aactgttctt tggtcagatc aattaccata
     3001 tgctttccgt cgctactgta tgtcaatgag tcataaacac tcttcaatcc aatatgaagg
     3061 tgtatcaaca atggctaaag aaggctacgg tgaaatgtca tgtatttcat gttgtgtatc
     3121 tccacttgat ccagaaaacg aagacaaacg tcacaacctt caatattttg gtgctcgtgt
     3181 taacgttatg aaagcacttc ttactggtct taacggtggt tatgatgatg ttcataaaga
     3241 ttacaaagta tttgacatcg acccaattcg tgacgaagta ctcaactttg acactgtaaa
     3301 agctaacttt gaaaaatctc ttgactggtt gactgatact tatgttgatg cgatgaatat
     3361 catccactac atgactgata aatacaacta tgaagctgta caaatggcat tcttaccttc
     3421 acatgtacgt gctaatatgg gatttggtat ctgtggtttt gcaaatactg tcgattcatt
     3481 atctgcgatt aaatacgcta ctgttaaacc aatccgtgac gaagatggtt acatctacga
     3541 ctacgaaaca gttggtgact tcccacgtta tggtgaagat gatgatcgtg ttgactcaat
     3601 cgctgaatgg ttacttgaag ccttccatgg acgccttgct aaacacaaac tttataaaga
     3661 tgctgaagca actgtttctc ttcttacaat cacatcaaac gttgcttact ctaaacaaac
     3721 tggtaactca ccggtacata aaggagtata tctcaatgaa gatggtagtg taaacctttc
     3781 taaagtagaa ttcttctcac caggtgctaa cccttctaac aaagctaaag gtggctggtt
     3841 acaaaacttg aattcacttt ctaaacttga tttcgctcat gcaaatgatg gtatttcatt
     3901 gacaactcaa gtttctccac gcgctcttgg taaaacattt gatgaacaag ttgataactt
     3961 ggtaacagtc cttgatggtt actttgaaaa cggtggtcaa cacgttaact tgaatgtcat
     4021 ggaccttaaa gatgtctatg acaagattat gaatggtgaa gatgttatcg ttcgtatctc
     4081 aggttactgt gtcaatacta aatatctaac tcctgagcaa aaaactgagt taacacaacg
     4141 tgtcttccat gaagtacttt caatggacga tgcattaact aactagtttg tattcgatga
     4201 agtaggtttg attatatcaa acctactttt atctatatta tcaatagtac taattacttc
     4261 tttatataac ctcaaggagg ggcctaatgt taaatcacaa atttcttcaa gttctaaaat
     4321 atgagggtgt cgtttcgata acaagctgga ccgaattagc tcctcacgtt acaaacactt
     4381 ggaatagcta cctgactatt acagatgacc agcgtatatt agctcctgca gcaggaatga
     4441 ctcatcttga aatgatctca ataataattc taaaattatt atgactctgg gcagccgtga
     4501 agtcgagggt cgtgatggtt atcaaggtac aggctttaga attgaaggaa ctgctaagct
     4561 attagaagca ggaagcgatt ttgaaattgt taaagaaaaa tacccttttc tgcgtaaagt
     4621 attggaagta actcccataa atgttatcca acttttataa aaaacttctc ctagaaaatt
     4681 ctaggagaag ttttttattc aaagtacgat gttatcgcct cttgtacatg tcgtcgtttc
     4741 ttaatccaaa gtggacggtc atcataggaa aaggttctgt ttgtcaaaaa aatagctgct
     4801 gcctgctttt gataatttag tgcaataaaa ggaccagtat aaccagtatg gtttatccaa
     4861 tccttatcta tatcccaagc taatgatctt gatttactca aactataatt attccacaaa
     4921 ggcttcgcaa aatcatcttt taaataatga atagaaaatc tttgtaaatc atcaatagtt
     4981 gaaaataaac ctgcagaacc agtatgcttt cctaatacct tagctttagg atcgtgtaca
     5041 ataccatcgt taataccggc tacagttgga acagcttcta ctctaggacc aaaagaggtt
     5101 tctttcatct gaaaaggagt gaaaatataa cgtttgaaaa gtttatccaa actatcccct
     5161 aatacctctt ctaacataaa accaagtaaa agaaaattaa tatcagtgta tttaaacgat
     5221 ttatcttcca aaaccttgat atgattaatg gcatctttca gctgagtagc attcaactga
     5281 tcacgatttg gaataaaagg gtcaattcca ctgctatgtg tagctaattg ccttacagtt
     5341 aacgtctgat gatgaaacgt agggtaataa taccttaatg gcttatcaat atcaatagtc
     5401 ccttgataaa ctaactttgc tagtaaagtt cccacgccga caacttttga tacactggct
     5461 agatcataga ccaatcccgc tttaactttc tcattgccat caattgtccc caagtaatgt
     5521 tcgtgccact ctccattttc ataaatagct aagcttgcac catgataaag attatctttg
     5581 atttgctcct taatgagttc taaaacggta ttagttttaa tcattctttt gaaatccaaa
     5641 tctcaatatt actagtgtca gagatgacta aaatcttttc tttagaatca aggtaaacat
     5701 caagtcccca tgattcaaaa gttgatttca agtcatttaa attagtatct tcattaacac
     5761 agcattctag tatctcaata tcccaagttt catttggagc aatttgcaaa tcttgtccct
     5821 gtgcttcttt gaaagataaa ttaattggga acttcccagc aaatacttta tcgtaaaaag
     5881 cttcagcttt cacggcatca ggtacattca aactaataga ttggaaagca aagtttgata
     5941 gacctgtagt gtcatctttt ttttctaact ctgggcgctc aattgccact aattgtgaca
     6001 aatcatcttc agcatgaagt aagaaaaggt ctccctcggg cgaaactgtt tcataggcat
     6061 aaccattttg accttgatat acttgaatgg cttgcgcacc atttgccaaa agcttttcaa
     6121 tatcttttgc atcttgagac ttaacaataa tctttgctaa cttcttagta ccattcaccg
     6181 cacgagtacg ataagttggg gattcctcaa taataaagct agcctctttg ttctgccatg
     6241 ctgagaaaac tgcgattgca ttttcttcac ttatcaattt aaatccaaga gactcctgat
     6301 aaaatgcaat attcaagtct ctattattaa cacgtaatac tgggtgttta aatgtcaatg
     6361 aatgaaataa cgtcataaat tcctccttgt tcagtctagt ctattttaga taattttaga
     6421 taatttagca agaatctttg tgctctcaat accataaagt caaattgaca gtttcactat
     6481 ttttttgtag actggatatt atgaatgaaa cactactatt acatgggatt cagcttattc
     6541 tgattatcgc catgattatc actttttatc agattgttag acatattagg tctcaaaaaa
     6601 taaatccttt caaacgattt ttcactggtt tgtggatcgg ttttgtaact gatgctctcg
     6661 atactcttgg tatcggctct tttgcaacaa cgaccacatt ttttaaacta actaagcttg
     6721 ttgaagacga tcgtaagata cccgcaacaa tgacagctgc acacgtactt ccagttttgt
     6781 tgcagtcact ctgttttatt tttgttgtaa aagtagaggc tttaacacta ataacaatgg
     6841 ctggtgcagc ttttattggg gcttttgttg gagctaaaat gactaaaaat tggcacgctc
     6901 ctactgttca acgtattcta ggaacactgc tcatcacagc tgctattatt atgctctatc
     6961 gtatgatcac taaccctggc gctggtattt cagattcagt gcatggtcta cacggcattt
     7021 ggttatttgt tggtatcgtc ttcaatttta ttattggtgt attaatgact atgggactag
     7081 gtaattatgc tcccgaactt atcttcttct ccctaatggg actgagtcct gcagttgcta
     7141 tgcctgtcat gatgctagat gcagctatga taatgacagc ttctagtaca caatttatca
     7201 agagtggtcg tgttaattgg aacggctttg ccggtctggt tactggggga atattaggcg
     7261 ttattgtcgc tgttctcttc ttaactaacc ttgatcttaa cagcttgaaa acactggttg
     7321 taggtattgt tttatttact ggagcaatgt taatacgttc ttcctttaaa aaaactatgc
     7381 ccaaaagtca tcattaaacg aaatcagcta gcaagactgg ctgattttta tatttaaaac
     7441 aaaaaaagac cggtaaaccg atctttcttt aactattaaa gatataatag tttgaacaag
     7501 gctacagcga gtatacttgc tacgattggt gctaaaactg gcacccatgc ataccaccat
     7561 tttgaatctt ctttagcttg tccaagaatt tgttttggta ataagcggtg aacaatacgt
     7621 ggaccaaggt cacgcgcagg gttcaatgca ggtccagtcg atccacctaa agaagcaact
     7681 agagtcatta ccaagaaacc aatacctaag tgagctacag caagtgaacc agttacgtat
     7741 ggtgaaatcc ttgtcgctgc tgtcgtttgg tcataaccag cttgaacaag tttaccaaca
     7801 agttctacac caaaatagtt cttcgttaaa gccaaagctc caaagaaaag aacaaaagaa
     7861 ccaacgaatt catttaagaa accattaatg taagccgctt tacgacttga tttctgtcca
     7921 tcatctaatg cagaaatagt tgaaaatgat ccaagaacat gatttggatt ttctgtctta
     7981 acaaagtaag gttgataaac cataacgaca actaactgtc caaatattgc tcctaaaatt
     8041 tgtgcaagaa tgtattgtcc aacgtgtgcc caagggaaaa gacctgaaaa tgccaatcca
     8101 agtgtgaaag ctgggttaat atggtttcca gagacattac caaacataag cgctggcatc
     8161 ataacaccta aaccataacc aattgcaata ataatccaac ctgaattatt tccttttgtg
     8221 ccttttaagt caacgttagc tacagcccca tttcctaaga tgataagaaa agcggttgcg
     8281 ataaattctg tgatatactt cacagtccaa gtgatttcga tcaatccttg tttcctcccc
     8341 aaaaaatatt tacaacatta accattctac caagtaagct taatattgta aagcttatca
     8401 caaaataaaa ttaataaaat ttttaaccaa atatatttga taaaaaatag agtactaggt
     8461 tcggactacc tagtactcta ttttatttag tttataaggt tttcttacta tcacgaacga
     8521 ctagcatatc aactttcgaa tgacgtaaaa tgtattctga agttgagccg atcaataggc
     8581 tttcaaaagt gtttaatcct gttgcgccta ccataattaa atctgctttt tcccttatgg
     8641 ggatatcctt tgccaaaaga gtcttaggat ttccgatttc agtaattttt ttaatctttg
     8701 taaggccttt tgaatgtgct aatttctcaa aataggctag cacatctgct gactcttgtt
     8761 cttgcctatc aaaaacatag tcactaaaga ctccctcact ttggtaagcg tgagcatcta
     8821 taacatgcgt tagcagtaat tcagcatcat tacgaagagc aacatttatc cctttttcta
     8881 ccgccaattc tgactcgtaa gacccatcaa tagcaataag aatacgctca tatttttgtg
     8941 tcatagctta attcctccct tttatatagt tttagtttca acttgtctag atgattttaa
     9001 gaaatataga ataccacaaa caacaggaat aatcgctgct atccagaata attctctaaa
     9061 ggattgcaca ccagctccaa gaaaaccatc tttaacaagt cccaaaatgt aaggtccagc
     9121 acctaaccct aaatcaagac ctatcatgta agttgacatg gctgtattga aacgatgttc
     9181 ctcaacacct ttgattgatg ctgcttggcc acaagacata aaggtgccat aaccaaaacc
     9241 aatcaaagca cctgatagaa ggtaggtaac acttcccata gcactcccta ataaagcaag
     9301 tcccaaagtt aagaacagat aacttggata aagcacccac ttatctccct tagcgtccat
     9361 taatcttccc atagatggtc tggttaaagt gatgacaagt gcataaacaa taaagaaata
     9421 agctcctact gtcatcaaat taatttctgt tgtatataat ttttggaaac ctaacacgga
     9481 agcataggag atacccatca aaaatgcaat aattgtgata aaaattgctt ttttctcaat
     9541 gaaactatca atagtccatg attttgattt agctaactgt tctggattta aagtaatatt
     9601 tttgactggg aaaacaaatg ctcccaacac tacaatcgca attaaaatac tacataatac
     9661 aataaccatt ttaaagttaa tatgaaggtt gtctagcata aatgttccta caaaaggacc
     9721 aatagctgcg gctaaacttg ttgatagacc gtaaaagtta atcccctcac ctcttttatc
     9781 agctggtata taggctgtta caatagtatt agttgctgtt gacacgacgc cataaccaaa
     9841 accatttagg aaacgaacta aatacattac tccgatactt ggcatataaa aataagctaa
     9901 agttgttagt aagtaaaaaa tagcccctcc acgtaaaact aacttacgtc ctagaacttc
     9961 taattgctta ccaaatataa gacgagcaat caaagtccct acaatataaa tccccgttgc
    10021 taatcctgct tggctagtgc taacacctaa ctctttagtc gcaataaaag ctatgataac
    10081 ggtaaacaaa taatagacca tataaacaat aaagtttaaa atagtaatgc ctataaaatg
    10141 tttgttgaat aatttgtctt ccaatgcaaa tggctccttt tttcaatttc attttcataa
    10201 acattttaca aaaatattag aagatacgat atttcaatgg tataatataa gtgctagtat
    10261 cagactgcat taacaaatgt taaggtggtc tatcaaacct aaaacatttg gaggaagtca
    10321 tgaatcatca aattttacaa aattacatta atagtcataa tcttccaatt atcgaaaaag
    10381 actaccataa ataccttaca tttgaaagtt tagaagaaga ctatacttat atcctaaaag
    10441 atggtattgt taaacaaagt gttctctcta aatatggtac tgagttcaat ctgcgttatg
    10501 tgacaggctt agaaatcact tctatcctaa atacagacta ctcccaacac atgggagaac
    10561 cttacaacgt tcgaatcgaa tccgaaactg cccactttta caaagtacgt cgctctacct
    10621 tcctaaaaga tatcaataat gatattgaac tgcaaggata tgtgaaagat ttctatcata
    10681 accgccttga aaaatctatg aaaaaaatgc aatgtatgct cactaatggt cgaattggtg
    10741 ctatctccac acaattatat gacctcagta agatgtttgg tgaagaacga gataacggtg
    10801 atatctatat taattttgtt atcacgaacg aagaacttgg taaattttgc ggaatatcca
    10861 caggaagtag cgtgagccgc attttgaaac aactaaaaga cgatcatatt attcgtattg
    10921 aaaaacaaca tatcattatt acaaatgttg agaaattaaa agatcatatt gtattttaat
    10981 ataacaatag aaaggatata ttatgcgtta taatcaattc agctacattc caactaaacc
    11041 taacgaagct tttgaagagc tcaaaggact aggttttcca ttaaacaaaa agaattctga
    11101 taaagctaat ttggaagctt ttctcagaca ttctttttta aatcaaactg atactgatta
    11161 cgctcttagt ctccttatcg ttgatgcaaa aaccgatgct ctaacctttt ttaaatcaaa
    11221 tagtgactta acactagaaa atttacaatg gatttattta cagttattag gctttatccc
    11281 ttttgtagac tttaaagacc ctaaagcatt cttacaagat attaacttcc cagtctcata
    11341 tgacaatatt tttcaaagtc tacatcactt actcgcctgt cgtggaaaat ctggcaatac
    11401 attaatcgac caattagttg ctgatggttt acttcatgca gataatcact accatttttt
    11461 caatgggaag tctctggcca ctttcaatac taaccaattg attcgcgaag ttgtctatgt
    11521 tgaaacatcc ttagatacta tgtctagtgg tgaacatgat ttagtaaaag ttaacattat
    11581 cagacccact accgagcata ctatccctac gatgatgaca gctagcccct atcatcaagg
    11641 tatcaatgat cctgccgcag accaaaaaac ataccaaatg gagggtgcgc tagcagttaa
    11701 acaacctaaa cacatacaag ttgacacaaa accatttaaa gaagaagtaa aacatccttc
    11761 aaaattaccc atcagccctg caactgaaag cttcacacac attgacagtt atagtctcaa
    11821 tgactatttt ctttctcggg gttttgctaa tatatacgtt tcaggtgtgg gtactgctgg
    11881 ctctacgggt ttcatgacca gtggggatta ccaacaaata caaagcttta aagcagtcat
    11941 tgattggtta aatggtaagg ttactgcatt cacaagtcat aaacgagata aacaagtcaa
    12001 ggctaattgg tcaaatggcc ttgtagcaac cacaggtaaa tcttatcttg gtaccatgtc
    12061 aactggttta gcaacaactg gcgttgaggg gctgaaagtc attatcgctg aagccgcaat
    12121 ctccacatgg tatgattatt atcgagaaaa tgggcttgtg tgtagtccag gcggctaccc
    12181 cggtgaagat ttagacgttt taacagaatt aacatactca cgaaacctct tagctggtga
    12241 ttacatcaaa aacaacgatt gctatcaagc attgttaaat gaacaatcaa aagcaattga
    12301 ccgtcaaagt ggggattaca accaatactg gcatgaccgt aattacctaa ctcacgtcaa
    12361 taatgtcaaa agtcgagtag tttacactca tggactacag gattggaatg ttaagccaag
    12421 acatgtctat aaagttttca atgcattgcc tcaaaccatc aaaaaacacc tttttttaca
    12481 tcaaggtcaa catgtgtata tgcataattg gcagtcgatt gattttcgtg aaagcatgaa
    12541 tgccttacta agccaagaac tacttggcat tgacaatcat ttccaattag aagaggtcat
    12601 ttggcaagat aatactactg agcaaacttg gcaagtttta gatgctttcg gaggaaacca
    12661 tcaagagcaa attggtttag gtgatagtaa aaaacttatt gataaccatt atgacaaaga
    12721 agcctttgat acttattgta aagacttcaa tgtgttcaaa aatgatcttt tcaagggaaa
    12781 taataaaacc aatcaaatca ctattaatct tcctctaaag aaaaattatc tcctgaatgg
    12841 acagtgcaaa ctccatctac gtgttaaaac tagtgacaaa aaggccattt tatcagccca
    12901 aatcttagac tatggtccta aaaaacgatt caaagataca ccaaccatca cattcttaaa
    12961 cagccttgat aatggtaaaa attttgccag agaagcttta cgtgaactcc cgtttactaa
    13021 agatcattat cgtgtcatca gtaaaggtgt cttgaacctt caaaatcgta cagacttact
    13081 tacaattgag gctatcgagc cagaacaatg gtttgatatc gagtttagcc tccaaccaag
    13141 tatatatcaa ttgagtaaag gtgataatct aaggattatc ctttatacaa ctgattttga
    13201 acataccatt cgagataatg ctagttactc tataacagta gatttaagtc aatcttattt
    13261 aactatccca actaatcaag gaaattaact tatgaaactt ctcactaaag aacggtttga
    13321 tgattctcaa cacttttggt accagatcaa tttattacaa gagagtaact tcggagcagt
    13381 ttttgaccat gataataaaa acattccaca ggttgttgca actattgttg atgatttaca
    13441 aggttccgga agttcgaatt atttctggta ttttggcaat actactgata cttccatcct
    13501 tatgattgct catttaaatc gaaaattcta tattcaggtt aatttaaaga actttgactt
    13561 tgcactcaat ttaatagcta taaataattg gaagagtctc ctccaaactc aactcgaagc
    13621 tctaaacgat accctagcaa tatttcaata aataaggtag aatggagtga caaagcaacg
    13681 cgagggagac tgattaatgt catcttattg gaataactat cctgaactta aaaaaaatat
    13741 tgatgaaacc aatcaactaa ttcaagaaag aatacaggtc agaaataaag atattgaagc
    13801 ggcgctaagc caactcacag ctgcgggagg aaaacagctc agaccagcat tcttttacct
    13861 tttttctcaa cttggtaata aggagaatca agatactcag caactaaaga aaatcgctgc
    13921 ttctttagaa atccttcacg ttgctacatt aatccatgat gatgtcattg atgactcacc
    13981 actaagacgt ggaaatatga ccattcaaag caagtttggc aaagacatcg cagtttacac
    14041 tggggattta cttttcacag tctttttcga tcttatttta gaatctatgg ctgatacacc
    14101 atttatgagg attaatgcaa aatctatgcg taaaattctc atgggagaat tggaccagat
    14161 gcaccttcgt tacaatcaac aacaaggtat ccatcactat ttacgtgcga tttcaggtaa
    14221 gacagccgaa ctctttaaat tagctagcaa agaaggagct tactttggtg gtgcagagaa
    14281 ggaggttgtt cgtctagcag gccatatcgg atttaacatt ggtatgacat tccaaatttt
    14341 ggatgatatc ctggattata ctgcagataa aaaaacattt aacaagcctg tcttagagga
    14401 tttagcacaa ggcatttaca gccttcctct acttcttgcc attgaagaaa atcctgatat
    14461 tttcaaacct attttagata aaaaaacaga tatggctact gaagacatgg aaaaaattgc
    14521 ttatctcgtc gtttcccata gaggtgttga caaagctcgc catctagctc gtaaatttac
    14581 tgaaaaagct attagtgaca taaataagct accccagagc tctgcaaaaa aacagttgct
    14641 acaattaact aattaccttt taaaacgcaa aatttaaata ataaaaaaac attccacaat
    14701 gctagaaaag cagttaggga atgttttttt attatcattt atttatcgca cctatcaatc
    14761 atcatagatc accatcatca gcggctttca gctgacggta acgttggcta ctttgagaca
    14821 attcttgagg agaaccttcc aactctaatt gcccattttc tataaataag atacgatcag
    14881 catgttcaat accttttaag tgatgtgtaa tccaaactaa ggtcttacct tccaattctt
    14941 tcataaatac ccttagtaag gcttgttcag taataggatc aagtccaaca gttggctcat
    15001 ctaagataac aattgggaca tcttttagta agattctagc caaagcaatt ctatgccttt
    15061 cgccacctga aaacctaagt ccagcttcat caaccattgt atagagacca tctgataaat
    15121 cagtgaccat ctctttcaat ccaactcgtt caagaacttt ccatacatct tcttcactag
    15181 catcttggtt tccaatgcga atgttattta gcagggttgt attaaaaagg tagggcgctt
    15241 gttgtatcac tccaatatag ttagaaatgc aatcaccaac tattgaaaca tcagcaccgc
    15301 ctagggtaat ctccccttga cttgctttca agtcgccacg aagtagactg gctaaggtac
    15361 tcttgccaga accactacgc cctaaaatag caattttttc tccttcttta atatccaaat
    15421 ctaaatgatg caaaacccat ttctcttgtg gcttatactg gaaacttaaa ttcttgacgg
    15481 aaaaatcgta tggcttatta ggcaactgat tttgagttgt ttcaaaatac gtttctgata
    15541 attcattaag acgatgaatt gaatcagaat atttatttgt ttcttgagca gcagcagaca
    15601 aaccagcaaa tgcctctgac aagggaaata ctgttagaac aaatgctgca atccagttag
    15661 cttcgccacc tctgtgacca ataaactgat tactagccca aataataacc aaaatagcta
    15721 gaaagccaaa aactaattcc acaataagag ctctacgatt atcaaaggaa cgaatttttt
    15781 tctgaacagc catgagttca ctttccgaac gctcatgcaa ggcgacgtac tcttgaccac
    15841 gttgactaaa aatccagtct gaaataccta agacattatc cgtcaaatca gtataaagat
    15901 gattttttaa ttctttttca cgagtctgac gagcaccatt agctaaaatc gaccaaagtg
    15961 gaaaaagaaa aatcataata gctaaataaa gcaacatcat taaagcaaac catagagaaa
    16021 agaagcctgt tgcaataata ataaagctat ataatatcca cgctataatc gttggaaata
    16081 ttgtcctaag atacaaattt tgcaaataat taatatcctc cgctaaaagc cccataacat
    16141 ctcctaaacg aaaatcacgc ttcataaaga tagcattgct ttctaaggaa tggtaaagct
    16201 tcaagcgcag ctgtgaagtc attcgtaata cccaattgtg actcgttagg cgttctatat
    16261 aacgaaaaac aggacgtcca atgccaaaag cacgtgttag tacaattgga acatatacta
    16321 gaagaatatt actcggtagt gaagctgact tactaatcag atagcccgaa ttaaacataa
    16381 gagcactggc agaaaagaaa gtcataaaac caaggaaaag agcaattact aaggaaactt
    16441 tatattgttt gaaaaatggt tttatccatt gatctgtctt taattcttta aacaaaggga
    16501 ttttaaacat catttcctcc catagcatgc ttcagttgat agagatatcc ttttttagct
    16561 aataattcct gataagagcc tacttctgca actctcccct tctccataac gactataaca
    16621 tccatttggt tcaaccagtg aagacgatga gttgcaaaga taactaaacg atctgacatc
    16681 agtggcaaca ttttctcttt taattctaac tctgtttcaa tatctaaatg agcagttggt
    16741 tcatcaaaaa ccataatacg acgattttga tcaagaaaag cacgagccag agctatacgc
    16801 tgggcttgtc ctccactaag cggacgcgct ccattaccaa tacgcgtttc taaaccgtca
    16861 ggtaactcgc ttagcaagct atctaaacct accatatgaa tcgctcttac cacctcttcg
    16921 tcactagcat tgggagtata gaaggtaata ttatcacgta atgacatttc aaaaacataa
    16981 ggagtttgag gaatatagat catctgttta tgccaatctt cttgatcaag gttagtcact
    17041 tctctaccat ccacttttaa agaaccatta tcaggcccta aaaaaccact cagaaggtta
    17101 attaagctag acttacctga accactaact cctactaaag caaccttttg atgtcctcta
    17161 aatcgtaagg ttacatcctc aacaacacgt ttttggtcgt acgcaatagc aatgttttct
    17221 aaatccaaac gactctcttt agaccaatca tctatgacta attgctcacc tttaatacca
    17281 gttttgttta gaactttttg aatcgcttga aatgcatttt tcccgtctaa cgttgcatga
    17341 taatcactag aaaaatctct tacgggtaaa aagtattcag gggataaaat caaaattgtt
    17401 aaagccggta aaaggtaaat ctgctcattt aataatcgta aacctagaaa aacagcaacg
    17461 atagcaatgg ataaagttgt aaaaaagtct aaagcaaacg ttgataaaat accaattttc
    17521 aaagtactca ttgttgcttt acgaaaagat tcactagttt gataaatgct tttaccataa
    17581 cgtttactaa gaccaaaata tttgagagta tcaatgcctc gtaatgaatc taaaaaatga
    17641 ttagaaagta cttgatagga ttcatattgt ttatctgctt tagcttgagc cgcatagcct
    17701 aaaataatca taaagatgat aattaaagga aaaacgataa gtaaaattgc acctgattca
    17761 atatctaaat agaaaatata agctagaata atccatggga taatagacat gttaatcatc
    17821 ttattcaaga ctaaacgtaa ataattttca actaaagaaa cgccatctaa tgccattgta
    17881 acaacattac cagttccttg ttcttgaacg actttaggac ctagatcaaa taacttatct
    17941 aataattgac gtctcaaaag actagattgt gctgttgaaa agtcatcaag ttttctattt
    18001 ttgatgtaat caataacatg acggcctaaa tatgagacca taaataacaa aacataaacc
    18061 gtttgagaag aaagtttttg cccttcccac aaaccagtta tagaaagact caaataataa
    18121 gcttgtccga taataaagat tgcttgaaga acatcaagtc ccgcaagcaa tcctaacaac
    18181 ttatgaatac ctgacaagcg cataaccgct ttatctagca ttatttttct cctgtcacaa
    18241 tcactggcaa ggtaatacgt tttctaaaga tataatatgc ccatgctgta taagctagaa
    18301 caaagggaac tagagtaatt gcgacaatcg acattacttt taaagtatat ggcgttgagg
    18361 atgcattttg aatcagcaaa tcatatttag ggctaattga tgaaatcata actcgtggga
    18421 atagaccttg gaacaacaga acaaccactg aaacaagact gagaccactc gctaaaaatg
    18481 ctgtcatctc tgctccttta aaagttgaag catgcgctaa tacagctaat acaacaataa
    18541 ccaaaaccat aattgtcgta actatcggat gattgctaaa gaaatctgtc ttgaaataca
    18601 agagaactgc aaacaaggct aaaccaagat agagtaccca ataaagcaac tgagcataat
    18661 tacgggcacg ctctctaatt ggcccttctg tcttcaatgc aatgtaattt aaaccatgaa
    18721 gataggctag caataccatt gcaactccac ccaccaacga gaatatatta aagtaatcac
    18781 tgaattgagc tgataagtta ccactggcat caagtggcat accttgaatc aaacttataa
    18841 acataatccc aaagaagaat ggaacaatag cggaaccaat tgttaaggtc cagttccaaa
    18901 actgcttttt ctctgcagga actttatgtc taaattcgaa ggaaacacca cgaataatta
    18961 atccaaagag aatcgtaagg agaatcaaat agtaaccact aaatagtgaa gcataccagt
    19021 atggaaaaga agcaaacatt gctccaccac cggtaagaag ccaaacttca ttaccatccc
    19081 aaactgggcc aatggtttct actacttgat ctttttcatg ctcattatgt gttaaagttt
    19141 gaacagccat cccaacccca aagtcgaatc cttcaaggaa gaagaatcct gaaaatagga
    19201 ggccgattag gaaaaaccaa aagaattgta aagcactcat ttagtatgct cctttctcaa
    19261 agggatcaat tgaagtatat gcaccatgat gtccgtgttc atcttcatac tctggccctt
    19321 tcttcaattc tcttattgtt aggcttatca tcatcaccgc taaaccacca aacaacaaga
    19381 aatacacagt attggaaaca aataatgatg caactgaaac gtttggagag acagagtcct
    19441 taatcttgaa aagaccatag acagtccaag ggtatcgtcc ttgttctgtc acaatccaac
    19501 caaatgtatt ggctaagaat ggtgcaaagg ttgttaaagc aacaatccaa agcatccatt
    19561 tgtgtttata aagaattggt tttttcttac gagttaaaaa gagtcctaaa acagaaacac
    19621 ccataatagc tgttcccatc gccgccattg tacggaaacc atagaaaagt aggttaacca
    19681 ttggatagta gttatcttta ccatacttag caaccaattc tttattagct gtatccatac
    19741 ctttcacaga accagatggt ttcccgtaag ataaaatact caacatataa ggaatcttaa
    19801 tcccaaaaac ttgtttgtgt tctgcttcat ttgcccaagc aactacactc caagctgcag
    19861 gatcacccga atcttcataa tcaccttcca tagcagcaaa tttcattggt tgatcgtgaa
    19921 tcaaggcttt catttgtaaa tccccaactc ccataactga aattgaacca agaagtgcta
    19981 ctactaaacc aatacgaacc gatttcttat aaagttcaac cgttgtatct ttcagctgtt
    20041 ctgattttaa gaggcggaag gctgacatac cagcaaccac tgtaccaccc atcgtaatgg
    20101 caccaaaaat aacatgggta aattcataga agaactgtgg attttttaca agtgcaagaa
    20161 aatcagtcat ttgcgctcta ccgtttacaa cttcataacc aacaggatgt tgcatgaagc
    20221 tattagcagt aagaatccac atcgctgaca ttaatgagcc aaaaaccact agccaaatga
    20281 atgtcacatg taatttttta ctaatttttt tgttatccca tgtaaacatc cataaaccaa
    20341 ggaaagtaga ttccataaag aaagctaata aagcttcaat agccaatgga gctccaaaaa
    20401 tatcacctac gaaacgtgaa tagtctgacc agttcattcc aaattggaac tcttgaatga
    20461 tacctgtcac aacaccaacc gcaaaactaa ggagcatgat attaccccaa aattttgtca
    20521 attttttata ctcttcattt tttgtgataa catacattgt ctccataata gcaaccacta
    20581 ggcatgtacc aattgtaaat ggtacaaaga aaaagtggaa aactgtagtc atcgcaaatt
    20641 gaaaacgagc taaagtttca attgtcatac aaaacctctt attttgtttt aatcatttct
    20701 tttatagaaa tatctgcaag agctagtttc ctagcttcgt aacaattggt aaataactta
    20761 ctttaatatc ttaatgatat aaatcaaaac gtcccttagc tagaagttct ttaagtccac
    20821 ccatatcaac aagtgatttg ttcatgataa tcttcttcat aaccgacgca gggtattttt
    20881 taattttagt ttttccaaca acaccaagtc catgcgtatt tcctactgaa gcaactgtgc
    20941 cttgcggact atacgaaaag tcttctgtag cttcaccttt gatttgatgt aaaaggtttt
    21001 tagcaacatg agctcccata cgagttgcaa tttgagctgt tgttgggaat gggcgaccag
    21061 actcagtgtc cataaatgca gacacatcac cgataacata aacattatcg tattttggat
    21121 cacgcaaatc actgttgacc attacacgtc cacgacgttg atcaaatcct gattctccca
    21181 taaccggact tccactaaca cctgttgtcc aaatgatagt acctgcggta atagatttga
    21241 gttcttcatc ctctttacta gtaccataaa caacttcacc cggtttgatt tccttaatca
    21301 tacttccaag catcaaatta atacctaaat ccttaattaa gttgacacca tattgcgcta
    21361 attcatcatc aaacattggt aggatgcgag ttgctgcctc tacacaaata atttcaattt
    21421 gttcaggttt tacacccgct aaagcagcat attttggtcg ctcatcaacc atagctccgg
    21481 ctaactcaat acctgtaaaa ccagcaccac atactaagag tttgagaaga ttttcatctt
    21541 ttgtctcacg gtacaacttc attgtattaa caatgtgatt atggatgttt tctgcttgcg
    21601 aaatattggt catctgcaaa gcattctcca tagcaccctt gatgccaaaa gtttcagata
    21661 caaagcctaa ggcaactacg acataatcat aatggagttc accgttattt ttaactgtta
    21721 ctgttttatt ttcagcatcg actctaagca cctcatcttg catgaagtta accttattag
    21781 tgttgataac atccttaatt gggaatgtaa ttttttcttt tggctgagag cctgcagcta
    21841 cttcatgtaa ttctgtagct tcgtagtgat aatcattcat gtcaacaagg gtaatatgaa
    21901 aatcacctga ttgtttttgt aaattacgaa ctgctttcaa accggcataa ccagcaccta
    21961 aaactaggat ttctttcatg ataaaactcc tctaaaaaat gattccaatt agatacgtta
    22021 agacttgggt tgcagatccc agtgctagaa tacgtaccgc acaaacaaat gtttctctct
    22081 taacctgttt ttgccacaat agttttgtct gcttataaat aaatggtatt aataacaaac
    22141 tcaagagtac tgtaacaggc gcaagtccaa aaagaaattc aaaaacaatt gccaataaag
    22201 ctatactatt agaaatagca aacagcaaga gtccacctct tatccctgta taatgtacaa
    22261 gggtatagcg atgatttttt tcatcttctt ctttgtcgca cagattattc gccaacatta
    22321 ggttggcaat ccataaggtg ttcggcaagg aaatcaagaa aattttgctt aagttgagga
    22381 tatcccacga aaagttttga taagtgttta gatatacaca aattaaacta atcataaatc
    22441 ccattgtcaa gccagaaaat acttctccta agggcagact agataaaggt cttggaccaa
    22501 aggaatataa aacaccaatt ccaaaacaaa aaagtcccat ccataaaaga ggcagaccga
    22561 cttgggaaac taaaactaaa ccaatcatac tagaagtaat gactaaactt gccataataa
    22621 cttctatttg gcgtagggat aggttctcac gaccaataat gttagtgtca ttcttataat
    22681 ccaaatccac agcattccga taatcattgt aattattcca aatgtcaacg aacatattga
    22741 ataaaaacat agcaacgaag aataaaccga catatacagg gtgtactgaa ttgtagtaat
    22801 aagcactaaa acacaaacca atcaaaaatg gtaaaacact agctgttttg gctttcattt
    22861 ctaccaactc tagaaagatt ggtaatgtca ttattaaagt cctctctata cataatcttc
    22921 aacaatacta ttataatgca atttctacca tttttaaaac aatagggctt gatttttgat
    22981 taaaaataca aaaaattttt cttaaaatga gttaatcaca cttagaacac taaatactat
    23041 tttgaggagt cctattcatc acactatgcc atgtaatatt cggaaaaata gtattgcaac
    23101 cctcaactat cccttatgtt ggcaccagcc tttgaaagtc ctctgattgc actgcttagt
    23161 tagtattttc atcagctgta tgatctctaa gagccatcag catactaaaa aaactagtat
    23221 tacactttga aaagtgtaac actagctcca atttgtttta aatagcttaa ctatttaatc
    23281 attaatctac tttatatatt taagcattta aagttgaatg ttggttgagg atacctgaga
    23341 gagaaccttg aatataattt ggcaatactt gtttttgagc acgttcatag atttctgaat
    23401 ctgttaactg acgttctgat ttggggcttg atacttgcgg tgtcactgct gcattttgtt
    23461 gtttgtaaag tgttgaatgc tggttaagga tacctgagag agaaccttgg atatattttg
    23521 gtaatacttg tttttgggca ctttcataaa gttctgaatc tgttaactga cgttctgatt
    23581 tggggcttga tacttgcggt gtcactgctg cattttgttg tttgtaaagt gttgaatgct
    23641 ggttaaggat acctgagaga gaaccttgga tatatcttgg caatacttgt ttttgggcac
    23701 gttcataaag ttctgaatct gttaactgac gttctgcttt tggaggtgtt gcttgcggtg
    23761 ctgtgaaagt tgaagctgaa acaccttgtg tagaaccaag agctacgaat gctcctccag
    23821 caaacattgc tgagacaaga ccaagtttca aatagttagc agttgagacg ttgttgactt
    23881 ttttttctgt aataagtttg ttcataagaa ttacctcttt cttttgggtt atttatttta
    23941 tgaactaagt ataacaacaa gaaataaagt aaggttaaac taaatataaa ataattctta
    24001 agtcattctt aggaaaccat aaaatcatac actaacaaaa aatttggtat ctagctcaaa
    24061 agtaaaagta gaataaaggc ttttacttct gagctagtaa tgaataaatt atatttgcat
    24121 ctagtttgga attagcccta gtgtattata atgatcccta caaatatgtg ccaaacacct
    24181 tcttcatata tacaagattg atttaacatt cctataccct taatggtggg cacattctct
    24241 tcatgttcca tatagaaatc aacagataat tccgtattgt ccctattatt aaattttcca
    24301 gttaatgata aattatatca tccaccttga ggtgtcctat taccaatctg ttcaaccttt
    24361 acacttgacg aatcgaattg aacagattct gtcttatcat tctgtgattt agaacatcaa
    24421 caatttcttg ttcatgttct ttgaggtaag ctagttgttc ttctttaacg tgcttttatc
    24481 tttcgtactt actgatactt gagagcattt ttttgaccct acttcttact aatcagctga
    24541 cgtccctata agatgaatca taaacacata gctgccacta accattttaa ccttttatgc
    24601 atacataatt cttcttgata tcccatacca ttatctttag tataaacact taataaaaca
    24661 agcatttaac aactaaggtt aataaaattc aataaatcta atccttctac ttactttagt
    24721 ttatcacttt tttctctcat cctatgctat aatagtaagg tatcaaaatt taggagaata
    24781 tagtcttatg atgaatatgc aaaatatgat gcgtcaagca caaaagcttc aaaaacaaat
    24841 ggaacaaacg caagctgatt tagcagctag ccagtttact ggaaaatctg ctcaagagct
    24901 cgttaccgtg acatttacag gcgataaaaa gctaatttca attgattaca aagaagctgt
    24961 ggtagaccct gaagatattg aaacactaca agacatgaca actcaagcta tcaatgacgc
    25021 tttaagccaa gttgacgatg ctacaaaaaa aattatggga gcatttgcgg gtaaaatgcc
    25081 attctaaaaa ataaatcaca tttactgaat cacctgtaaa tgtgattttt atttcaaact
    25141 tctttcattt tttcacgaaa acaagtttta ttttcaaaaa atatttgcaa aaaatcagca
    25201 gagaggtata ataacattga aaatattaga gagaaggtga ctaaccatgg ttatggatag
    25261 tctaatcata aaacagttga ttaagagtac atttgatata cccttacaag tcacttatcc
    25321 aaatgggaat attgaaactt ataatggtag caatccccac gtcaaactta aactaaacaa
    25381 aaatttctcc gtctcggagc tatcaaaaga cccttccata gtattgggtg aagctgttat
    25441 ggatggagac attgaaattt atggttctat acaagaatta attttatcag cttaccgctg
    25501 tggagacagc tttctacgta atagtaaatt tagcaaattg attcctaaac aatttcatga
    25561 taaaaaacac agtaaatccg acattcaaaa gcattatgac atcggaaatg atttctataa
    25621 actgtggctt gatgatacca tgacttattc atgcgcctat tttaaacatg aaaatgatag
    25681 tctagagcaa gctcaactaa acaaagtaca ccatattctt aataaattaa atgctcagcc
    25741 gggagggaaa ttactagaca tcggttgtgg ctgggggaca cttatcatta ctgctgctaa
    25801 agaatatggc ttaaatgcga caggtatcac cttaagtgaa gagcaggctt ctttcattac
    25861 taagcgtatc aaggaagaag ggttggaaaa taaagtaaca gtcctcatta aagattatcg
    25921 tgacattcgt gaaacatacg attatatcac tagtgttggt atgtttgaac atgtaggaaa
    25981 agaaaattta tctcaatact tccaaaccat ttctaaaaga ctcaacatca atggtctagc
    26041 tcttattcat ggtattactg gacaagtggg tggtaatcat ggtagtggaa ctaattcttg
    26101 gattaataag tatatcttcc caggaggata tatccctcgt ttaactgaaa atttaaatca
    26161 tatagctagt gctggacttc aaattgctga cttagaaccg cttcgtcgtc actatcaaaa
    26221 gactctcgaa ttatggacaa aaaatttcca caatgccctt cctgaagttc agaaaactca
    26281 tgataagcgt tttatcaata tgtgggacct ctatcttcaa tcttgtgcgg cctcttttga
    26341 atctggcaat attgatattt ttcaatactt gctttcaaaa ggtgtttcaa aagacactat
    26401 gcctatgaca cgcgattata tgtattctgc taattgatat agttaatatt atagtattcc
    26461 aacttattct aaataaaaag gactctcttg aattcaaggg agttctttaa cgttcttctc
    26521 tggctaactc tatacaatgt gacttttggt gacaatgtgg acagaatact tttcgcgtct
    26581 taggggtatg tttcgctaga ttaaatgctt gatatgtcgg tgtaaaggtt tgatgacaat
    26641 ttggacaaag ataaacaacc tgttgtttaa aataccaaac cataccattc atagctatta
    26701 agaatactgt tactgctatc cacataagcc atctttgatt tagagaaact gctgctatta
    26761 ttaacgtaat atagaccata ataatcataa cgacttgtag cactatgctt ttttgtagta
    26821 taccccattt tcttttgttt tcttccataa gcacaatgtc agaaagatag cctaatgttt
    26881 tactatcttg tttttctaca gtttttagta ggttgacagc tttgtcaagt ttgtcttttt
    26941 tactatcaat agctagcctt aattctctta tctacacttg taaaaaaagc tctaaaatct
    27001 ggctagcatt gtcttcagta aatagatttt tgatattatc aattgaaaaa tctaaatctc
    27061 gtaaataaat aatttgtcta agcttttcta aatcagcaat actataaacc ctcctacctc
    27121 cttcagatag ttctgatggg gataaaattc ctcttttgtc atagtactga acagtacgaa
    27181 cagtaactcc tgcttcttta gctaagtcac cagtggaata tttttctgac atgtttcaca
    27241 cctcctttaa tatagtaaac acttctttat cagaaacacc tttactatat caaatgacct
    27301 aaggtaataa gcaagtaata acgctaagta tttttaactt taaataaaac tacaacaatt
    27361 aatcaaacaa atttcctaaa gtcgcaaatg ggctatcaac tgctacctct ttttgctgtt
    27421 ttaaatagat tttattttca tccaagtcca gaaatttttc ataaacacgc gcagtcatac
    27481 gggcatcctc taagctatta tgtgctttac cctttattcc taaaaagtca gcaacagtag
    27541 tcaatttaag gttgacaata ccatttaaat ctgtactgcg tctcacaaaa gcttcatcat
    27601 ataagtccac ctgatattga ctagttagat ccaaaccatt ttcaactaaa aggggtaaat
    27661 ccgatttata cccattataa ccaatcagtg gtgtatctcc aacaaaactc tgaaagtctg
    27721 tcaaaactat ctcaatttta ggagccccta taatatctct agctgtaata cctgttaaac
    27781 cattaataaa gctttgtaat ggaacttttg tgtgaacata agtatcaaat aaagcaatct
    27841 cttgatgatt agaatattta acggccgaca cttgaataat atggctatgc tcaccaaccg
    27901 tgttaaactc taaatcaaaa gctatatatt cttgtaattg tttcattatt tctcctaaaa
    27961 agcgcaagga aactccttac gcttttattc tattttttat tgaagaaacg tccaaagaaa
    28021 ctttttttaa cagcttctaa ttcttgaatt ttttcttctt tggcttcttg aatctcctca
    28081 acctctgctt tagcttcatt taactcaagt tgcagacgtt gcgtatcttc catagcagtt
    28141 aaagtcaatt gctgttgttg atctaattgc ttatctttct ctgctatttg aatatctttt
    28201 acatgtaact gctcgtcttt tgaagcaatc tgaatatctt ttgcttttaa ctgttcatat
    28261 aaacgtgtga tttcagtatt cttctcatca acgagaattt ctaaaagttc tcgtcttgat
    28321 gcttcttcgt caattggttc ctcttccaaa atagtttttt tatagatttc ttccaattta
    28381 atcaagccat cacgattaac aactgtaacg cctttactat tcttttccac acattcttca
    28441 gggagtgttt taactctgtt attcatcgct tgacgactaa cccctaaaat ttcagctaat
    28501 tcactaaccg tcttttcaac tgtcataact tcctcttttg tactgatttt cttataaaag
    28561 aatatcacaa tcctaatcta gtgtcaattt actgtcaact taaagtgtca aacaagacat
    28621 tttgacgtct atttctctat ttatatgcta gaataagatt atgaagcatt atgacacaat
    28681 aattataggt ggtggacctg ctggaatgat ggcagctatt tcctcaaatt tttacggcaa
    28741 caaaacactt cttattgaga aaaataaacg tttaggcaaa aaactagctg gaacaggtgg
    28801 tggccgctgt aatgttacca ataatggaaa ccttgatgag ctcttagctg gtatacctgg
    28861 aaatggccgt ttcctatata gcgtcttctc acaatttgat aaccatgata ttattaactt
    28921 ctttcaagac aacggcgtca cacttaaaga agaagatcat gggcgtatgt tccctactac
    28981 tgataaatct cgtactatca ttaacgccct tgaaaataaa ataaaagaac ttggtggtca
    29041 aattatgact gacacagaag ttgtctctgt caaaaagata ggagattcat tctatatcaa
    29101 aactaaagat acacagttcg catccgataa acttatcgta acaactggtg gtaaatccta
    29161 tccgtctaca ggttctactg gtttcggtca tgacattgct cgtcacttta aattagaagt
    29221 cacagatatg gaagccgccg aaagccctct tttaactgat tttccccata aaaaactaca
    29281 aggtatctcc ttagatgatg tcactttgtc atttgaaaag catattatta cacacgatct
    29341 tctctttact cattttggat tatcaggacc agcagccttg cgaatttcta gctttgtcaa
    29401 aggcggagaa actatatacc tagatgtttt gcccaatatc tctgttaaag aattggaaat
    29461 acacttccaa aatgaaagag aaaaatcttt gaaaaacgct cttaaaatac ttcttcctga
    29521 gcgtttagct gaattttacg ctgaagactt acccgaaaaa gtaaaacaag tatctgtaaa
    29581 agacttagaa atgttaattc aaaaacttaa aaaacttcct attttagtaa ctggcaagat
    29641 gtctctagca aaatcctttg taacaaaagg gggagttgat cttaaagaaa taaaccccaa
    29701 aacacttgaa agtaaaaagg tagctggttt acattttgct ggtgaagtcc tagacatcaa
    29761 tgctcatacg ggagggttta acattacttc tgccttatgt acaggctggg tagctggtag
    29821 cttacactat taatacatga aagctgaaca attccgtcag cttttttata tagaatttaa
    29881 agataatatt tggctcttaa ggcttctttg attttttcca attcatctgg taaatttacc
    29941 tgatgaatag catacccaat ctgataaggg gatactataa aacgaggaac aattttacaa
    30001 ataagacccc catcatgctt atagaaaaag agattataag atttacagcg ccctcccaag
    30061 aattcattta aaatataatg acacaaaatt ggtaaactat ctgcaatcaa aatagcgttt
    30121 tccaatttgg atactttaat tgtgaactct gtgaaatcaa gcataggacg attggcaata
    30181 ctaatctctg cttgttgact cgtttccgaa caaacctcga taccatcaaa ccaatctaag
    30241 ttcaacgttt cataagcatc tacgtttttt aagccaacaa tttggctatg aggatgtcgt
    30301 aaacttccgc cagataaggg cccaaaattt cgataaagaa cgaccgaacg gtaatgacca
    30361 ctttcttcca ttaattgcca ttttgacaaa agataatcta acaattggcg catctttgac
    30421 tcactataaa ctgaaatatc cccattgtga tcatttgatt caatcaccaa tgtttgatag
    30481 gtttcttcta aagttgggta tttattttct acccagatca tatcgtcttt cacgtctagg
    30541 atttttccta attggctttt atcacaaaat ggacatttgt ctttattgct gggtttttgt
    30601 ttcgcgatat tagggttaaa tcggatatat ctttcattca tactgttatt gtaacacatt
    30661 catcaccatt acaatataat acttatccag ttaacatttt ccttgcattc ttagctgaaa
    30721 agagtataat agaatcatgt cttgtttacc ggttaattac cagttaacta ggcaccctgt
    30781 tttgggatag aaaaatcttt ttgggaaagg agaaatcatg gctaagaaat caaaaattgc
    30841 taaatttcaa aaacagcaaa aactcgtaga acaatatgct gaactacgtc gtgagttaaa
    30901 agaaaagggt gattacgaag cacttcgtaa acttcctaaa gattctaacc ctaaccgact
    30961 taaaaatcgt gatttaatcg atggccgtcc tcacgcttac atgcgtaaat tcggtatgag
    31021 tcgtattaac ttccgtaacc tagcttacaa aggccaaatt cctggcatta aaaaagcaag
    31081 ctggtaattc gtgaagacaa atagttactt tttatgacta ttctattgag tgtggacgaa
    31141 tagttaatac attgtaatta tttaacctgt tgagtattta ctcagctatt acaaacttct
    31201 tcaaaatttt attatcatag gaatgtagga tctcatcaat ccttaaatga ctaataataa
    31261 aagatgaaaa aatttttctt atctcaaatc atagatgcca atttatttat tttcaaaaag
    31321 cccggtatgc caagataccg agctttttat tattataaat actgataaaa ttcttttagt
    31381 taatcccaaa aacaagacta ttattttgta atagtcacta ctgaataata tttcttttat
    31441 taaaaactca caagacattt cttaaagcag ttacatcttc gcgactagtt gaccaactag
    31501 ttgctagacg tattacagta tgatggtcat catatttttc ccaaaaacta taagctacat
    31561 tctttgctaa atctgctagt tttgtatttt ctacaataat aaattgttga ttagttggtg
    31621 attttaaata gaatgagtaa cctttttctt ctaaaatgat ttttaattcc tctgctaagt
    31681 cgatcgcatg ctttccaatt tttaagtaaa ggttatccgt aaagaaacga tcaaaagcaa
    31741 gccccagcaa tcttcccttg gctaataacg caccatgctg ctttacaatt gtagtaaact
    31801 gttttggacg atttttcttt gtgaaaacaa ctgcttcccc agcaagagcc cccattttgg
    31861 ttccaccaat ataaaaaaca tctgataagg ctgcaatcgt tgggaaatca acatctgtat
    31921 ctttcgcagc caaaccatac cccagacgag caccgtcaat aaataagggg atttgataat
    31981 gtttacaaat cttcgataac tcttctagtt ctgctttact ataaagtgtt ccatattctg
    32041 ttggatgtga tatataaacc attcctggaa aaaccatgtg ttggtaatta ccatcagcat
    32101 aaaaagtctc aatataagtt gcgacttcgc ttgcgagtaa tttcccatta tgagaaggta
    32161 aagtcaatac cttatgccct gaaaattcta tggctcctgc ttcatgacta ctcacatgac
    32221 ctgtttctgc agcaataact ccttcataag aagcaagcat actactgata actacttgat
    32281 ttgtttgagt accacccact aaaaactcaa cttcagcttg tggacaggaa cagacttctc
    32341 gaattttatc tgccgcttgt tgacagtaac tatctgtccc atatcccgat agaccttgat
    32401 cattagtttc aataagggca tttaaaagct ctggatgagc tcctttatta taatcatttt
    32461 caaaatgtaa cattccttaa ctcctttatt agttaaaaaa atagcgacaa tatcatttgc
    32521 taatattttt gaatattcaa tcattataac aaataaaaaa tatcaaaaca agcgttttga
    32581 tattatgaac acctataaac tctcaaaagc taaactaggt ttggcattca attttaaaga
    32641 agcaaaattc tctttttcaa actctatcgc tgctgctaat gcaatcattc ctgcattatc
    32701 cccacataat ctaagaggag gaataaccac atcaatatca ggagaaatat cagtagccaa
    32761 gcgttcccga agtccttgat tagctgcaac tccaccagca acgactaaag ttttcactgg
    32821 atactttaac aaagcttttt gagttttggc caataaaata tccaaaacag ccgcctgaaa
    32881 ggaagcacat aaatcttcaa gaaccaatgc ttcacccttt tgttctgcat tatgatgtaa
    32941 attgataaat gcagatttta atccagaaaa agaaaattca agatgatctt ctttgatcat
    33001 cgctctagga aaatggtaag tatcttgacc cttatgagct aactgatcaa tctcgcgacc
    33061 tgctggataa gttaagccca taacacggcc tactttatca taagcttctc caacagcatc
    33121 atcccgagtt tctcctacta ttttgtaatc tcccggttca gaaacatata ctaattctgt
    33181 atgtccccca ctgacaagca aagctaacaa tgggtattga agttccttaa cgtcacgtgc
    33241 tgccattaaa tgacctgcca tgtggtttat aggaattaga ggtaatttat ttgcccaagc
    33301 gaaagctttt gctgcagcca tacctaccaa taaggctcct actaatcccg gaccatatgt
    33361 tacagcaaca gcatccaaat cgctagcaac aatacctgct tcttgaagag catcctcaaa
    33421 acaggttgtt actacttcaa cgtgatgacg gcttgccact tcaggaacaa caccaccaaa
    33481 acgtttgtga ctttcaactt gacttgcaat aatattagct agtaactctt tatcattttt
    33541 taaaatagca acactagttt catcacatga actttccact gctaaaatat atctatcttt
    33601 cataatgata ttaatccatc aatatcgtct tttccattag tattgaggac tattcatatc
    33661 ctttctatca tcaactattt atcccttgcg catcactata gcatcttcaa ttggatcatg
    33721 gtaataattt ttgcgctttc ttaaaactac aaaaccacac ctctcataaa gtttttgtgc
    33781 tacgatattt gaataacgaa cctcaagaaa aactggcacc tctatatcgg cgatcattga
    33841 cattaactgg taagcataac ctttaccttg ataatttttt ttaacagcaa tattagtaat
    33901 ttctacttca cctaccaatt gttgaagagc caaaaaacca atgacttcac cgtcattata
    33961 aacaaaaaaa taatctgttg aatctttttt taaatcttct gaaatctgcg ataaactcca
    34021 cggagatttg tcatagacat cagatagtac gctccatata gccagtgctt gttctgatga
    34081 ttccatgtca aactctctta atatattctt ctgtattcgt ttcacagtgg ttttttaacc
    34141 aattttcctc agcttcaaca cgttttaagt atcgtggaac aaacgcatct acattaacgc
    34201 ttttcatttt ttgtcctttg cgcccaattg ctactgcaca aggtaaagtt tctgtaattt
    34261 tagcatgtgg taaggctttc ttaatctgat caaaaaatgc tgcaacctct ccgacaaaat
    34321 gaacattggc tttattcccc acctcttgta agacttcttc aagagaagtg tgacagtctg
    34381 gtttaacagt atcaccattt tgatagaaac caacataaac attgttacgt cgtgcatcta
    34441 taagtggtac caataaatca ttttctgaaa atccatttgt taaagcgtac aggctagata
    34501 ctccaactaa gtcaatctta agcgtataag ctagcatttt tgctgtagca acagctacac
    34561 gtaagcccgt ataagatcct ggaccctctg atactacgat acggtccaaa tcttgaggtt
    34621 ctaaatcaat tgattgcatt aaaaaatcaa tggctggcat caaattaatg ctatgattct
    34681 ttttgatatt gatagtgaca gtcgctaaac attccatatt gtttagtaca gccactgata
    34741 gtgctttgct tgaagtatca aaggctaaaa ctttcatcat ttaacttccc ttacttgatt
    34801 ctacctctta ttttatgata taatcatgat aattgcaacg aatcaaggtt aattctatta
    34861 ctctataaaa acttaataat ggtttgtcat tgtctcactt aacggcattg acccattttt
    34921 ttatagttag aaaaaccgag agataagaaa ggattattat gatttataaa gttttctatc
    34981 aagaaacaaa agaacgcaac ccacgtcgtg agcaaaccaa aacactttac gttactattg
    35041 atgcagctaa tgaattagaa ggtcgcattg ctgcacgtaa actagttgaa gaaaatacag
    35101 cttacaatat cgaattcatc gagttacttt cagacaaaca ccttgaatat gaaaaagaaa
    35161 ctggtgtttt cgagctcact gagttctaga aaggagttaa acataatagc tagagtaatc
    35221 tacccattat gataaactta ttatgtcaaa tatcaactta aaacccgaag aagttggggt
    35281 ttatgcgatt ggtggtctcg gtgaaatcgg aaaaaatact tacggtatcg aatatcaaga
    35341 cgaaattatt attgtagacg ctggtattaa attccctgaa gatgatcttt tgggaataga
    35401 ttatgttatc ccagactatt catatatcgt tgaaaacatt gaccgtatca aggcccttgt
    35461 tataactcat gggcacgagg accacatcgg tggtattcct tttctattaa aacaagctaa
    35521 tttaccgatt tatgcaggtc ctctcgctct tgctctaatc aaaggtaagt tagaagaaca
    35581 tggtttactt cgtgacgcta cattatacga aatccacgct aatacagagc tgacttttaa
    35641 aaacttaagc gttacatttt tccgcactac tcactccatt cctgaaccat taggtattgt
    35701 tattcatacc cctcaaggta aggttatttg tacaggtgac ttcaaatttg actttacacc
    35761 tgttggggag cccgctgact tacatcgtat ggctgctcta ggtgaagatg gtgtcctctg
    35821 ccttttatca gactcgacaa atgctgaagt tccaaccttc actaattccg aaaaaattgt
    35881 tgggcaatct attatgaaga ttatcgaagg tatcgaggga cgtattattt tcgcctcttt
    35941 tgcttcaaac atcttccgac tccaacaggc tgctgaagct gctgttaaaa ctggtcgtaa
    36001 aattgctgtt tttggacggt caatggaaaa ggcaattgtt aacggtattg aacttggcta
    36061 tattaaagtt ccaaaaggta ccttcattga gccaagcgaa cttaaaaatc ttcatgctag
    36121 tgaagtttta attatgtgta caggtagtca aggtgagtct atggcagcac ttgctcgtat
    36181 cgctaatggt actcatcgtc aagttacttt gcagcctggt gatactgtga ttttctcatc
    36241 aagcccaatc cctggtaata caacaagtgt caataaatta atcaatacca ttcaagaagc
    36301 tggtgtcgat gttattcatg gtaaaattaa caatatccat acctccggcc atggtggtca
    36361 acaagaacaa aaattaatgt tacgcttgat taaaccaaag tatttcatgc ccgtacacgg
    36421 tgagtatcgc atgcaaaaag tccatgctgg tttagcagtt gacacaggta ttcctaaaga
    36481 gaatatcttc attatggaaa acggtgatgt tttagcttta acgagcgact ctgctcgtat
    36541 tgctggacat ttcaatgctc aagatatcta cgttgatggg aatggtattg gcgatattgg
    36601 tgcggcagtt ctacgtgacc gccacgactt atctgaagat ggtgttgtcc ttgccgttgc
    36661 taccgttgat ttcgattcaa aaatgatttt agctggacca gacatcctta gccgaggctt
    36721 tatctatatg agagaatctg gggaccttat tcgcgaaagc caacatatct tattcaatgc
    36781 tattcgtatt gcactgaaaa ataaagatgc aagtatccaa tcagtaaatg gagctattgt
    36841 taatgcctta cgcccattct tatatgaaaa aactgagcgt gagcctatca ttattcctat
    36901 ggtccttacc cctgataaat aaacaaaaga acggaaaata ctttctccgt tctttttaat
    36961 tgcctatatt agcaaaataa gttaatatat caaattactt ctgggataac tatagaatat
    37021 aaaagaactc caaacagtat cgtactttta taccgtctat gtgaggagtt ctttatagat
    37081 taattcggat aaatgtagct aactacccct ggtgttactg tattttgtgg atcaaaccaa
    37141 ccacgataat tatcaatcca ttgatgacct gcataattag attctaaaac ctgaatttta
    37201 ttagtgacag gatctacagc tgtaacataa gccacatgtc cataactacc atcagtccaa
    37261 catacgatag aacccacgcg cggttgtgtc cctactgtat aaccagctga tgcggcgctc
    37321 gcagcccaat caccaccgtt tccccaccag ttacctgccc atgttgccat ctcctttaca
    37381 ccccatgtgc attgacctag tggaaacgta ttactagctg gatgtgcttg agcaatttcc
    37441 tccgcacttg ctaggtagtc tacatctact gcatagttat atgtattcgc atctgcattt
    37501 gttgaagcaa atgaaaaagc ggacaccaaa gccactgttc caagcattgt tttagacttt
    37561 aattgtttta tgttcatgtt ttcctccatc tgatgactcc tagtttatca aatccatata
    37621 acagacaaat gaaaacctgt aacaagtttt aggcaatttt ttacaataat ttacaaaaaa
    37681 actagtttta gactttacaa aataaagcct aaaactagtt ataaacattc ttatgattaa
    37741 tataaatcta ggtaattatc aatttcccat tgtgaaacat aagtcgcata acttgcccat
    37801 tcaatacgct tagcgtctaa gaaattagta tagatatgag ttcctagggc cgcttttacc
    37861 acttcatctt cttcaagtgc tttaagagca ttatgaagag tagatggtaa atcaacaata
    37921 cctgcttgac gacgttcttc tacagtcata gcatagatat ttgtctcaat tggttctggt
    37981 gcttcaattt tattctcgat tccctcaagt cctgatccca aaagtactga taaagcaaga
    38041 taaggatttg ctgttgggtc aaccgagcgc aattctaggc gtgttcccat acctcgagat
    38101 gcaggtacac gaataagtgg cgaacgattg cgacctgccc aagcaatgta aactggtgct
    38161 tcgtatcctg gtaccaaacg tttataagaa ttaacagttg gattaatgat agctgtgtaa
    38221 ttgtaagcgt gtttcattaa cccacctaaa aagtagtagg catcctctga taattgcatt
    38281 ccacgtggat cttctggatc aaagaaagca ttgttccctt cattatcaaa aagagacata
    38341 ttacaatgca tacctgaacc attgatacca aacttaggtt ttgccataaa agtagcatat
    38401 aaaccatgct tacgagcaat tgttttaaca accaatttaa ataattgaat gttgtcacag
    38461 gcttttaaaa catcatcata tttaaagtca atttcatgct gtccaactgc tacttcatga
    38521 tgactagctt caacttcaaa gcccatttgt gttaagacat taacaatttc acgacgtgta
    38581 ttatctgcta agtctgttgg agctaagtca aagtagcctc ctttgtcatt aacatcaagt
    38641 gttgggttac cattttcatc cattttaaat aggaaaaatt caggttctgg tcctaagtta
    38701 aatgacttat agcccatctc ttgcatacgt ttcatattac gcttaagatt tccacgtgga
    38761 tctccggcaa aaggttctcc ttctgctgta tagatatcac agattagacc tgcaacagct
    38821 ccattttcat ctccccaagg aaagacaatc caagtatcta aatcaggata taaatacata
    38881 tcagattcat tgatacgaac aaagccctca atagaagatc catcaaacat tgctttattt
    38941 gataaaactt tgtctaattg ttcatctgta gccggaattt cgacattttt cattactcct
    39001 aagatatctg tgaacatcaa acggaggaag gtaacatttt tttccttaac ttcgcgacga
    39061 atatcttctg ctgtgatagt catatagtct ccttaatatc tagcttgact gcatgtttac
    39121 aaatgcatat ttccaatgtg ctgtgaagga cttgaaaaac gcccttgatt acggagctca
    39181 tcttgtaaaa gtcgacgaac ctcggcatct gataatgact tttgtttctc tttggactta
    39241 tgttgtcttt gcatgtattc atttttaata tcagaaatat gcaaaccatc gctaatgaag
    39301 tcttttattt ccagtaagcg gtccatatca ttcagtgaaa acatgcggcg gttaccttca
    39361 gtacgttcag gtgtgattaa cccttgatcc tcataatagc gaatttgtct ggcagtcaaa
    39421 tctgtcaact tcataacagc tccaatcggg aatacagcca ttgtacgcct aagttctctt
    39481 tctttcatga tggcctcctt tcattaaacc attataaact ttaaaaagtt tgatgtcaac
    39541 tctcatgtga gattttatga catcaaacta taatttgtta ttttttagtt ttttccgaag
    39601 tatttcaata tctgtattaa ccatcattgc aataaaaaca ccacaaaaag tttcaaaaat
    39661 acgtgcaaaa acatatagaa ttgtctctcc gcttgggata gataaagtta taataagtaa
    39721 tgcggctgtt cccccaataa tacctgattt attattacaa gcaacattaa tcatgataca
    39781 taacatggta caaataggaa caattaataa agtgacccaa aaagcttgat gaaatatctc
    39841 attgaatgca aaaaatacta aggagagtaa gccacctata ctattaccaa taattctaga
    39901 aaaaccaaag tgcacgcttt tatcaaagtc ttctcttaaa ctaaatacag cagttagagc
    39961 accaatttga agcccgttcc aaccaaataa atgaaaaacc aataaaacta aaaagaccga
    40021 taagcctgtt ttaaatgttc tcattccaaa tttaaacttt ttaatatcaa acttgtaatt
    40081 agataataat ttttttaacg ttttcatacc tatcccttca ctatatattc taacatatca
    40141 atccataaaa ccctttttgt aaacgtttta tttattacta gtatatcttt tataaaccaa
    40201 tttatatcaa atcgtcataa acttttgaaa attgcagtgt caaccaaata ccactatatc
    40261 taaaaactag taaatgataa atgaagtcaa ggaaagttca ataactctac attatcaact
    40321 aaaaaaagac atgcatcagc atgtccttta atataaatta taatgactat tatttttcag
    40381 tcaatgctgc caaacctggt aatactttac cttcgagcaa ttccatgctt gctccaccac
    40441 cagtagagat ccatgagaat ttgtcagcac gaccaaggtt gatagcagct gctgctgaat
    40501 caccaccacc gatgattgat ttaacgcctg gttgtttaac gatagcgtcc attacaccga
    40561 ttgtaccagc ttggaagtca gggttttcaa agacacccat aggtccgttc catacaactg
    40621 ttttagcacc agtaagtgct tcatcaaatt tagcgattga tttaggaccg atgtcaagac
    40681 caaggaaccc ttctgaaact gcttcacctt cagtgtcgcg aacttcagta taaccagcaa
    40741 atgcgtttgc ttcttttgag tcaactggca agatcaattt accgtttgat ttttcaagga
    40801 ggtctttagc aacatccaat ttgtcttctt ctacaagtga gttaccgatt tcgatacctt
    40861 gagctttgta gaatgtgtaa gtcataccac caccgataag aactttatca gctttttcaa
    40921 gaaggttttc gataacacca atcttatcag aaacttttga gccaccaaga atagctacga
    40981 atgggcgttc tggagtttca actgcttctt ggatgtaagc aatttcgttt tcaagaagga
    41041 aaccagctac agctttttca acgtttgctg aaatacctac gtttgatgca tgagcacggt
    41101 gtgctgtacc aaatgcatcg ttaacgaaga ttccatctcc aagtgaagcc cagtatttac
    41161 caagttcttc gtcattctta gattctttct taccgtcaac atcttcaaaa cgagtgtttt
    41221 caaccaaaag aacttgtcca tcttccaaag cattgattgc ttcttctaat tttgcaccac
    41281 gagtaacacc tgggaataca acatcttgac caagtttagc agctaaatca gcagctaccg
    41341 gtgcaagtga ttttccttct ttgtcagctt cttctttaac acgtccaagg tgagagaaga
    41401 ggatagcacg accaccttgt tcgatgatat acttgattgt tggaagagcc gcagtgatac
    41461 ggttgtcgtt agtgataacg ccgtctttca aaggcacatt aaagtcaaca cgaacgagga
    41521 cttttttacc tttcaaatca acgtctttaa cagtcaattt agccattagt aaaactcctt
    41581 atataatttg atattattta ttataacata tttttgtgaa cagttgtaat tattgattac
    41641 tttttcacaa atagatttca tagattttcc tagttgtttt taagagaatt cgtatactta
    41701 ttaatcatac gaatgcaaca ttttttgtcg ttctttcaat tgtttttgaa catccagatg
    41761 ttttccttga taaatagcac tttcccaaga accgtacata ggatttggga aaacaataaa
    41821 tttactacca aactcacttt gaagtttagt tagtagttgt tctctatctg tactagatga
    41881 tttagaaaaa tcagcaaaat caactaaatt atctccaaaa agcataatta aattggtatc
    41941 tttttgaact gcctgacggc gactctcttt agatttcatt ccttttttaa ggaaaagcaa
    42001 gtggtctttc ccttgaacag gtataccttc cttctctaaa ttttctttag tcgcatcaac
    42061 ttgagcatct gtacgatctg agacataata aatttttatt cccttttcat tagcatactt
    42121 caaaaattct ttggcacccg caacagcctt agctgatttc ttttgcaccc atttatccca
    42181 actctctggc gtgaaactag agccatcttt aatattcttt gcttgatatg ggctattatc
    42241 taaaactgtt tcatctaaat ctaagataat tgaatatggt ttttcactgg gcttttgtaa
    42301 ccaatcatct aacttcattt tagcaacatt ataaccttgt aaatataaag ccttggcttc
    42361 tgcagaattt tgataccaaa ggacagacat agtgttttcc ttagccaaca gtttattaga
    42421 acttagagaa atatttttaa atttcgtctt ttgctccacc ttatctggag acatatcagc
    42481 ttttttagta ctgtggtttg actgacatgc tgtcatcata aatacagcaa cagataacga
    42541 taaaactgaa aaaacttttg tagtcttcat aaaacttctc ctctatattt tttcaattaa
    42601 atcataccac tgcttacttt caaaatcaaa ccttatttta acttatagtc aatataattc
    42661 atttttcatt tattttctga taattttcta taataaaatg aaaaaacatt cgtattattt
    42721 tttatttata aaaataaaag agggataaat ccctctttta catcatatta attaaaaatt
    42781 attttgcaat ttttgcaaag tattcaagtg tacgaacaag ttgtgaagtg tatgacattt
    42841 cgttatcgta ccatgaaaca actttaacca attggttacc gtcaacagtt tgaactttag
    42901 tttgagtagc atcaaacaat gaaccgtatg aaataccaac gatatcagat gatacgattg
    42961 gatcttcagt ataaccgtat gaatcgttag ctgctgcttt catagctgca tttacttctt
    43021 cgacagttac gtctttttca agagttgcaa ccaattcagt tactgatcca gttggaacag
    43081 gaacacgttg tgcagcacca tcaagtttac cgttcaattc tgggataaca agtccgatag
    43141 cttttgcagc accagttgag ttaggaacga tgtttgcagc acctgcacga gcacgacgaa
    43201 ggtcaccacc acggtgtggt ccgtcaagga tcatttggtc accagtgtat gcgtggatag
    43261 tagtcatcaa accttgttta acaccaaagt tgtcttgtaa agctttagcc attggagcaa
    43321 gacagtttgt agtacatgaa gcacctgaga taactgtttc agttccatca aggatatcgt
    43381 ggttagtgtt gaaaacaact gttttaacgt cgtttccacc aggagctgtg ataacaactt
    43441 ttttagcacc attttcatgg atgtgttgtt cagctttttc ttttgatgca aagaaaccag
    43501 ttgcttcaag aacgatttct acgccatcag tagcccagtc aatgtttgct ggttcgcgtt
    43561 cagcagaaac tttaacaaat tgaccgttaa cttcgaatcc accttcttta acttcaacag
    43621 taccgtcgaa acgaccttga gttgtgtcat atttcaacaa gtgtgcaagc atgtttggat
    43681 ctgtaaggtc gttgatacga gtaacttcaa caccttctac gttttggatg cgacggaatg
    43741 caagacgacc gatacgaccg aaaccgttaa taccaacttt aactaccatt agtgatttcc
    43801 tccttatgaa aatctcaaat attttatttt agggatcccc ctaaccaatg tgaaaagatt
    43861 aacttgtaca tatgtgcaaa aatctttcaa cacaattatt atatcttttt tcgagaaaaa
    43921 ttgcaatgaa tttaccactt tcaaaaagaa aaaaagatac tctttcgagt acctttcata
    43981 agttttcaga aattattcac cagagttttt cttgatgatt tcttcttgaa ctgacttagg
    44041 aacatcttcg tagtggtcaa ataccatcat gaatgtgcca cgtccttgtg ttgcagaacg
    44101 aagaactgtt gcataaccaa acatttcagc aagtggaaca aaggcacgaa caacttgtgt
    44161 attaccacgt gcttccatac catcaacacg accgcgacgt gcagtcacgt gacccataac
    44221 gtcaccaaga ttgtcttctg gagctgtaat tgtaacaagc atcattggct caaggatagc
    44281 tggttgcgct gatttagcag cttctttaag tgcaagtgat gcagcaatct tgaaggctgt
    44341 ctcagatgag tcaacatcat ggtatgaacc atcgtaaagt ttagctttta cgtcaaccat
    44401 tggatatcca gcaagaacac cgtttgccat agattctaca agtccttttt caacggctgg
    44461 aataaattca cgaggaacca caccaccgac gatagcattt tcgaattcga atcctttacc
    44521 ttcttcgttt ggtgtaaatt caatccaaac atcaccaaat tgacctttac caccagattg
    44581 gcgtttgaag aatccacgtg cttgtgtaga agcgcggaat gtttcgcggt atgatacttg
    44641 aggtgcacca acgtttgctt caactttgaa ttcacgtttc atacggtcaa caaggacatc
    44701 aaggtgaagt tcacccattc ctgagataac tgtttcacca gtttcaacgt tagtttcaac
    44761 gcggaacgtt ggatcttctt cagcaagttt ttgaagggcg atacccattt tatcttggtc
    44821 agctttagat tttggctcaa ccatcaattg gataactggt tctggaactt caattgattc
    44881 aagaataact tttgattttt catcagtcaa tgaatcacct gtagttgtat ctttcaaacc
    44941 aacagccgct gcgatatccc cagcataaac agtttcaatt tcttgacgac tatttgcgtg
    45001 catttgtaag atacgtccga tacgttcacg tttaccttta gatgtattca acacgtatga
    45061 acctgaatta agaacacctg agtacacacg gaagaatgtt aagcgaccca cgaaaggatc
    45121 tgtcatgatc ttgaaggcaa gcgctgcaaa tggctcttca tctgaagcag gacgtgtttc
    45181 ttcttcgtca gtatcagggt taataccttt gattgcagga atatcaagtg ggcttggaag
    45241 gtaatcaata acagcatcaa gcatcaattg aacaccttta ttcttgaagg ctgaaccaca
    45301 caatacaggg tagaattcaa cattgatagt tgctttacga atagcagcca tcaactcttc
    45361 gttagtgatt tcttcacctt caaggtattt catcatcaaa tcttcatcag tatcagcaac
    45421 tgcttctact aatttttcac gatattcatt tgcttgatca acatactctg ctggaatatc
    45481 ttcttcaagg atatctgtac caaggtcatt agtatagatt tcagctttca ttttgattaa
    45541 gtcaataata ccacggaaat cgtcttctga accaattggc aattgaattg ggtgtgcatt
    45601 cgcttgaaga cggtcatgaa gtgattgtac tgaatagagg aagtcagcac cgattttatc
    45661 cattttatta gcaaaaacga tacgtggaac tccgtattct gttgcttgac gccaaactgt
    45721 ttcagtttga ggttctacac ctgattgagc atcaagaact gttaccgcac catcaagtac
    45781 gcgaagtgaa cgttgaactt caattgtgaa gtccacgtgt cctggtgtat cgatgatgtt
    45841 aacacggtga ccatcccatt gtgctgttgt tgcagctgat gtaattgtga taccacgttc
    45901 ttgttcttgt tccatccagt ccatttgtga agcaccttcg tgtgtttcac cgattttgtg
    45961 gattttacca gtatagtaaa gaatacgctc agttgttgtt gttttaccag catcgacgtg
    46021 agccatgata ccgatattac gagttttttc aagtgaaaat tcgcgagcca tttggtttct
    46081 cctatatttt aaattattta cttctttatt ataacactat ttataaaaaa cggataggca
    46141 ggacctaccc gttctaattg ctaacattgc tatctaccaa acaatttgtt tagtagctat
    46201 gataagtctc aaaaagagag taatcttacc aacggaagtg tgcaaaggca cggttagctt
    46261 cagccatttt gtgagtatct tcacgtttct taactgatgc tcctgtgttg tttgcagcat
    46321 ccatgatttc ttttgcaaga cgatctttca tagtgtgttc accacgagca cgtgatgcgt
    46381 taaccaacca acgaagtcct aaagttgtac gacgttctgg acgaacttcg acaggaactt
    46441 ggtagtttga tccaccaaca cggcgtgcac gtacctcaag tacaggcatg atgttttcca
    46501 tagctgtttc gaacacttca agagcatctg ttccagtagc ttctttaatt gcttcaaaag
    46561 catcatatac gatagttgca gcagtaccac gtttaccatc aagcattaca cggttgataa
    46621 gacgtgtaac gatttttgag ttatacaatg gatctggcaa tacttcgcgt ttaggcgctt
    46681 gatttttacg actcattttc tcttatcccc cttctaatta ccctttagga cgtttagcac
    46741 cgtatttaga acggccttgt ttacgatcag ctacacctgc agtatcaagt gcaccacgaa
    46801 cgatatggta acgtacccct ggaaggtctt ttacacgtcc accacggata agcacaacac
    46861 tgtgttcttg caagttgtgt ccgatacctg gaatgtaagc agtaacttca ataaggttgc
    46921 tcaaacgtac acgagcaaat ttacgaaggg cagagttagg ttttttaggt gtcatagttc
    46981 caacacgagt tgcaacacca cgtttttgtg gtgcagataa tttagtgtga acttttctgt
    47041 gactgttgta accaatattc aaagcaggtg aatctgattt ttcaacttta gatttacgtg
    47101 gtttacgtac caactggtta attgtaggca tctacatttc tcctgtaagt tttttatttt
    47161 tggttgatat agcacttggt gacagcctat atctgcgtgt acttttgcaa caattgtcag
    47221 cacgtctctg tacacttttg agagaccaaa agtaaaagta ccgtttatca tagtatcatt
    47281 ttacacataa tgtgtcaagc ttttattact tttacaagca aaaagaatca aggatgtcac
    47341 cttgactctt taatcaaata tattccccgc ttctacagag actctattat tttttacatc
    47401 aatttcagat actctcagaa gagatttata ggccatcttc tcacgttgtt cctgagcatt
    47461 ttctgcaaat acagcgacac ctactaatgt gctatcgaat tctgagagta aactaatcat
    47521 ccccgaaaca gtacctccac ctttcaagaa atcatcaact attaaaactc gactgttagg
    47581 ttttaagcta cgcttagaaa gaaacatttt ttcaatacgg ccacttgaac cactagcgta
    47641 gtttactgac acagtagaac cttccgtaat cttcaaatca cgtctcacaa taacaaaagg
    47701 aacatctaaa acatttgcta ctgcgtttgc taaaggaaca ccttttgttg caaccgtcat
    47761 gacagcatca attttttgac ctctgtaagc gttcgctata atgcgaccaa tactttgtaa
    47821 catttttggt gtactgagta gatctgaaag ataaatgtaa cctcccggaa gaatacgatc
    47881 gttttcagat aaacgctgtc ttaattcttc aacgatttct ttcgcttctg cttcagcaat
    47941 agttggtgta aagataacac ctccacttgc accagtcaca gttttaatat ctccgatttg
    48001 agcttgttca aaagcttttt tgataatagc aatatcttct gaaatcgaag atttcgcggc
    48061 accgtattta ctagcaaatg tatttaaact agttaatgta tagggattat ttattaggta
    48121 atttgaaata acaaccatgc gttcgctacg tcttaatttc atattttctc cttattcata
    48181 gcattcttta catatagtat aacataacaa atgctaaaaa cgaacatttt attttaggtc
    48241 ttaattattc tacctaaatt atttaatgtt cggtttataa aaagaacggt tatccatggc
    48301 aaaaatgcga tttgtcattt caccttcgtt gacacggttt aaagctgttg tcatcatcat
    48361 catatttgca tcaatgttat cgatcatatg gataatttct gcctccatga ttctaggacg
    48421 aaccgggctt ccatattcta attgtccatg atgactcaga ataacatggc gtaacactgt
    48481 aacctcttct tttgtatcat caatattaag ctctgctagt atttttgtta attcctcatc
    48541 aataagtgaa atatgaccga taagattacc tcgaatagta tattctgtat tatcaggacc
    48601 cgataactca atgaccttgg ctaaatcatg tagcataata ccagcaaaca tcaaactttt
    48661 attaagttct ggatagatat ctccgatact atctgccaaa cgaaccattg ttgccgtgtg
    48721 atatgccaat cctgattcaa aagcgtggtg gtttgttttg gcggctggat aggtaaaaaa
    48781 ttccttattg tatttacgat aaagtgctct taccacacgc tgccaggttg cattttcaat
    48841 tttaaagagc atttgttcca tgtactctct cacgttgtca acattgattg gaggtttttc
    48901 tttaaaatct ctaggatcat taggttcacc atctttgatg tttcttaatg taatttggtt
    48961 gacttgggga gttccattat aaacttcacg acgccctttc atatgaacaa ttttaccggc
    49021 tacaaactct tctacattat aggtctgtgc atcccacata tttcctgaaa tttcgccagt
    49081 atcatcttga aaggtaaagg caataaagtc tttacctgca cgcgttttac gaacttctgc
    49141 tttctttata aggtaaaacc cctcaaaaag ctcgtctttt ttcatttggt taattttcat
    49201 taatttcctc ttcttctgtt ataggaaggt ttaacaagct agtggttgct tgatcttgat
    49261 gttcttcaac tgtatttaag actctgataa tagcgtttgt ccgcgttgtg agtaaactat
    49321 caatactttt acttgctgta tttaattgtt tttgagcttt tgataacatg ccaccaaatt
    49381 ttccaaattc aaccttgaca ttccctaaaa ttttactaat atcattagca ttcttttgaa
    49441 tatttaaggt tttaaagcca acagataatg aatttagtaa agcagataag gttgacggtc
    49501 cagctactac aatattttcg tcacgtctta gactatcaaa gaatgttgca tttcttacca
    49561 cttcagaata gagcccttca gttggtaaga acatgatacc aaaatttgtc gtttcaggag
    49621 gatttaagta cttattgttt atatcttttg caaatttacg aatacttgcc agtaaagatt
    49681 tacgatagag ttctatttga accttgtcac ccaattcata agcatcttcc aatcggtaat
    49741 aatcttctag aggaaactta gagtcaatag gcaaatagat ataatctccc tgaccatttc
    49801 caggtaattt aatagcatat tcaacacgct cagaagagcc agacaccgta ggaaattctc
    49861 tctcatattg actaactgtc ataatatctt ctataatttg gccgagttgt aactccccta
    49921 atattcccct tgtcttagta tttgacaaga ctttgttcag tgtaccaaca tcttgggcaa
    49981 cagttttcat ctccccaaga ccttgattga cgctctctag ttgacgcgat acagtttcaa
    50041 aagaagtttg caaacgcgtt tgtagcgttt tttctagctt ttcctcaaca gtttggcgca
    50101 tttgatccaa gcgtttttca ttggaatctt gcattttctg aactgactga gataaattgg
    50161 agttaatcaa ttccaagcgt ttgtctgatc gatctcgact ctccgttaaa tgctgattta
    50221 attctgtacg catatcggta agcaactgat aaatttcctg ttgcatacgg tttagctgat
    50281 tagtcaacgt tagaatttga tctttttgcg ccacttctat ctgatatgtc agttggtctg
    50341 atagattatc agcattatct tcaagttgtt tttcaacgga ctttgttaat tccgtcattt
    50401 ttgggtaaga aattatcgtc actgtcagac tagctagtac aatgatcaaa atgatgacaa
    50461 gcatattaac ctctatcctt actataaata atgattaagt aaccatcttt gagctctact
    50521 tcaattggcg aagtcataaa ttcattactg gaatacattt ttttcttaaa ataattactt
    50581 ttgttcaatt catatttagc cccagaaatc tgcaaagtag actcactctc aggcatgaac
    50641 gagacatagg acatcccttc aatccttgag acttgatttt tcccggatgg tagataagtc
    50701 actatgtttt gttcatcttt gagtctgatt tgtgacataa atggttctag atctctgtca
    50761 cttggtaaaa agatattaga catcatatgg tcaatcctgc caccaaatgc tccaaatacg
    50821 gtcacctgag cttctggaaa ttctttgaaa atagttttta aagctaattc tgtatctgta
    50881 tcattttttt ctgcactagc agaaacgata ttgctacaat agtgtttgat ataaagcagt
    50941 tcatcttctg ttatagaatc aaaatcaccc actgccatgt ccaaagaaag cccatttttt
    51001 aataaaaata ggcttccacg gtcaattccg acaaaatagt caaaatcata ttcaaaataa
    51061 gttaagtcac cgccagcaaa taaagcaatt ttagtcatcg agcgccaccc ttaaggtttc
    51121 tacttgtgca ggtaaatcac ttgctttaaa gagataagaa cctgcaacaa agacatttgc
    51181 accagcctct gctgctgcct taattgtagt attatctata ccgccatcaa cttcaatatc
    51241 ataatgatga ccgtactctt tgcgccatgc tgcaactgtc tttacttttg acatcatttc
    51301 tggaataaag gcttgtccac caaagccagg attaacagtc ataattaaaa tctggtcaac
    51361 caaatctaag ataggaataa gagattctac gggtgttcct gggttaataa ccacacctgc
    51421 tttcattcct gcctctttta tcttttgaag cgcaccatgt atatgtttcg ttgcttcaac
    51481 atgaatggtc atgatatcag caccagcttg agcaaaagcc tcaatataac gttctggatc
    51541 aacaaccatc agatgacaat caaaaacaag tttactgtat ttacgcatgc tggatacaac
    51601 atccgcacca aaactaatat tagggacaaa ttgaccatcc atgatatcaa tatgaacgta
    51661 ttctgctgtt gtctcttcaa tacgtttcaa ttcgttagca aaattagcat agtctgctgc
    51721 taatatcgat ggggcaattt tattggttga catagaaacc tactttcttt taataacttt
    51781 cttatatgtt tcacgacggt tctcaatttc acttaaaaat tggaggtagt tatcatagcg
    51841 gacttgccat aactgacctt gagttaaggc ttcttttaca gcacatttag gctcatgtgt
    51901 atgcgtacac gagcggaatt tacaaaaatg actaagtcta cgaagctctg gaaaggattc
    51961 gtttaagtcc tctgcattat ccacttcata gtccaaagaa gaaaaaccag gagtgtctgc
    52021 aatttttcct ttatgaacgt tatagaaact aactgctctt gtggtgtgtc ggccacggcc
    52081 taaactaccc gaaatttcac cagtttcaag tttcaactca ggagcaattt tgttgagaag
    52141 cgttgatttt ccgacacctg tctggcccat aaaaacagtc actttatttg ctaacagagg
    52201 caagagttcc tcttgagaat aacagaaaac gtagcctata ttctgataaa gttcgcgaat
    52261 atcatcaata acaaccaaat cgtctaacaa atccaacttg gaaatataaa tgataggctg
    52321 aatcatctta tattctagaa gtactaagaa ccggtctaat aagttagcgt taaaatcagg
    52381 ttctttagca gacatgataa cgacagcttg atcaatatta acaataggtg gtcgtacgag
    52441 gctattttta cgctcttcaa ttgatagaat atatccttct gattgatctt gcgaagaaaa
    52501 ttcaacccaa tccccaacgt agggaatttg accttttttt ctaaaatttc cacgcgcacg
    52561 tgtctgataa acaacaccgt cagattccac ataataaaaa cctgctagcg acttgacaat
    52621 tctaccttgc aattgttctc cttaattgtt tagaatacca ctattatatc ataaaatgag
    52681 aaataagctc tactctcaga gataagctaa atagaaacta tttgcaggtg ttcgatttct
    52741 ctcaaataac ctcaatttta tgaattccca acataaaagc agtgatctta tttagataca
    52801 ctgcgctttt ttccaattac tattaaggca cctactgtaa tttctgatat actcgcccaa
    52861 tcaatagtcg ttttatctac gctgaataac aacggtgtca tatcaataaa agcttgacgt
    52921 tcttgttgag aacagttata gctttgaaca actttctgct cagaaataat ttcaaaattt
    52981 tctctaaagt gatctagaat tttttgatta gagtaatctt tctgtttcaa gtattgacta
    53041 gctttttctc gcaattcttg aacatgttca gctacaggaa caacctttac aaggatacca
    53101 tcatcactca gcacccgcct aaattcttta taatttgcag gagaaaaaat gtctaaaatg
    53161 atatcaattg atgagtcttg aataggaagg ttagctaaat cagaaacaaa ccatttcaca
    53221 agcctagact gatcactttt agcagctaat tgaatcgaat ctttagaaat atcaaaagcc
    53281 aagaaagtct tctcatgctt gttaactaat tgacgagaat aaaagccctc tccacaacca
    53341 atatctaaaa ccgaatgaaa ctgagaatta ttttccaaaa cctttgaaat agcttctaaa
    53401 atatgattat aatacccgtt ttctaagaca agctgccgat tttcaaatga ttttttatca
    53461 taatgttcat ctaccttctt tcctccaaga agattaacat atccaaattt ggataaatca
    53521 aaacaatgat tatcagagca tttgaggctg ttactttctt ttatcaaggg attttgacat
    53581 ttaggacaag cgaaataact tgcactatct tcaaaacgcc acttcttgtc aaccaacata
    53641 gtttatatcc caacctcttt caaggcatct gctaaacttg caaaatcagg gatagataag
    53701 gcttctccgc gaatagatgg cttaattttt gctatttcta atgctttttc caattttgct
    53761 ttagtatctt ctgattttcc aaaatgactc gttagattat tccaaagtgt tttacgacga
    53821 tgtacgaagg ctacttttga cactctgaaa aagaaatcct catcttgaac actcactact
    53881 ggttgatctc tacagaccat tttcaaaata gctgaatcta catttggagc aggaacaaaa
    53941 acagttctcg gaacgataaa tgatactttg gcagtcatat aatactgcac tgcgattgac
    54001 aaagaaccgt atgctttagt atttggcata gctgaaatac gatctgccac ttctttttgt
    54061 atcatgacca caaactccgc aaaaggaatc ttgctctcaa tcaagtgcat aaggattggt
    54121 gttgtgatgt agtaaggtag gtttgcgacc accttgatag gcagatcagg attcttgaaa
    54181 gcctgaatct gggtctgcaa atcagccttg aggatatcct gattgaccac ttggacattg
    54241 tcaaaacgcg caagcgtatc agcaaggata ggaatgagac gatcatcaat ctcaaaagcc
    54301 ataacctcag ctgcattctc agccaaaaac tctgtcaaag ccccgatacc aggaccaatc
    54361 tcaatcacgt tgacaccctt gtcaatctct gccgtgtcaa caatcttctg taaaatatta
    54421 gtatcggtca aaaagttctg accgaatgat ttcttaaatg taaacccatg acgctccaaa
    54481 atagcgcggg taacggtttt atctgcgatt ctcataattc ctctatttct taatattgat
    54541 catttttgaa ataacatttg aggctatttg tgatattaca actggattat accactactt
    54601 ccttttgcct ttactattct gagttataaa cttattttta attgcagttg cacaagcttc
    54661 ggttagtggt tctcctattg cttgcttata attaaatcca atcaactgca ttgtttttaa
    54721 agtatcttta ttcaataatg taaccaattg tttcattgtt cgttcctgtt cctcatttag
    54781 accagaaaaa ttatataaca atgagattcc ttgctcgaga gtattggata gatattcaat
    54841 atcctcatca tctatattaa ttttttcata ctcttcttta tatgactgtg caatgcgaat
    54901 tgcttgctcc cgttcagcta ataattcgtt aattaaatca tcataagtag ctctcaatgt
    54961 tgcagcatct tttacatcct ttattgattg aattttactg gttaccatag ttgcagtccc
    55021 tttgactgca agcgttgcta aatctacccc agctctagtc aatgcttccg ttacattata
    55081 ctccactatt tttctccttt tctattaata cccctccata cactcttcaa cttctgcctt
    55141 ggtcactccg aagagtctga ggcgtttgag gagttgttta ccatttgagt agccgatacg
    55201 gagttgattc ccaagatact cacggcgttt tcggctatcg ctagcagtaa tgaatcccca
    55261 acgaatgagg tctgcttggg tgatatcgaa atgatcttca tcatcgaaat gctgagtcac
    55321 ttttgagagt gccttctgca aatcttcaaa gctggcatgc tcgacaccca gagatcgtcc
    55381 cttggtcttt gagcctggct ttgcctcgtc tcgattgagg aaggcatggc ggactgtcgg
    55441 aatggcattc atgatgattt tacggatacg ctcgccatta taatctgggt ctgtaaagac
    55501 gataactccc cgcaaattgt gcaaacgttc gatacgctct aaatcatctt catcaattgc
    55561 tgaacctcta gtttcatagg tatcaacatt gtaaaagcgt cgcagattag cagtatcatc
    55621 tttcccttcg acaacgatca cttcttgaat atcaattttt ttcatcaagt ctaaatatcc
    55681 ttactgcatt ttggtatgta gcctccgcta cctcttccac agttatgcct cgtagctctg
    55741 ctattttttc aacgacatat ctcgtataag cggttttgtt ttctcgacct cttttaggta
    55801 caggagccaa ataaggagca tctgtttcca ctaatatctt atctaatggc aactctcgtg
    55861 cagcttcttg tacatctaat gccttcttaa aggtcacaac accagaaaag gaaatcatca
    55921 tccctaaatc aataaatttc tgagccattt ccaaagaacc agaaaaagag tgcattatac
    55981 caccaaaagg cccaactcca gattccttaa tgacttcata agtgtcttct aacgcatcac
    56041 gtgtatgaac gacaaaagga aggttatact cttttgaaag ttcaatttga cgcttaaaca
    56101 cctcgatctg aatatctttg ggatcctcca tccaataata atccagacca atttcaccaa
    56161 gtgcaataac cttgggattt tctaaatgac tgataatcat tgactcaata ttatcatcat
    56221 aagaaccagc ttcagttgga tgccatccta ttgtgctata tacctgtgca tattgggaag
    56281 ataattccaa agattttgaa atggtatctt ggtcaaagcc gacaacattc atttttgtaa
    56341 caccgagttc gctagctaaa ttaatttcct cgtctatctt tccttcaaaa ttttctacgt
    56401 taaggtgggt atgggtatca aatattttta tcatatcatc gttctttctt tgcttatcgt
    56461 agctacaagc attaatcttt attattataa cataagactg aagcattctg atcagaaaga
    56521 gtaaatcaaa aaagtcaaaa catttttgct ctgacttttg aatacttgct gtaaaaatcc
    56581 tctacaagtg tctagctaat ggtagagaaa ctagactata aatgattata tttttgctga
    56641 ttgctttcaa tagcctcctc aacctttgaa cgaaattctc cttttattgc ctcgtccaat
    56701 tgattagcaa ctttatgaat agctgtcttt gtgttgatgg ctacttcgtc tatttcttta
    56761 tgatgcgacg taataatatc gtgaaactta gatctcttgc gtggcgcgta cacgctatgt
    56821 atcaattctt tatccaattg tttttttaaa gtccttactg actccttacc agtcaggaca
    56881 tcccacatta aactcaataa tttctcctac ttcattaatt ctcttttctt ttccctttgc
    56941 aactcttcaa aggttagttc atgttctgaa cgttgccgaa tatagttagc tctatattct
    57001 tcttcttgag cggacaaggc atagtactct ttatggtaag cttgctctaa gtcgtcatga
    57061 taagaaccta aaatacgatt gagcgtcatc atatctgagc tatcattact atatctcaaa
    57121 tcatattcaa cagcgtcgta agtttcttga agcatattaa taatcttatt ttttcttact
    57181 gagagatcgt ctaatttttc ttcaaactgg caacaactat ggtgataggc atcatctaaa
    57241 tctgatattt ttctctgagt atcagctaat tgtttttcaa tgatatcaat cagtgccatt
    57301 gtttaaactc ccctgacaag ttactatctt gtcgcaacat agcttcaact cctttattaa
    57361 tttggttttc taattcaacg taagcgtgag aaatgctttc tgccgcttga gttttatttg
    57421 agaaaaaggt ttgagcttct ccgacaatgc tcgcatagct aacacctgac tgagcatacg
    57481 cttcttccaa ttcttgagga cttagttcag taactcctgt aggagctact tttaaagatt
    57541 gataaagctc ttctgcttta gcaactgctt tttgctcaac ttttttaact tcttcaactg
    57601 cttcttttga tatcgttacc aaaccactag agatcacttt tgcttgttga ctatctagga
    57661 aaatagcttg attcttacta acttcttttc ccgtactttt aaagctacta agtttcgctt
    57721 taatagcatg cattctttga ttcacattcg tatgaactac tgaatagttt tctttgtaag
    57781 caaatttatc ttctgctaat tttaaagaac catctggatt gtactggtag cctccccaaa
    57841 tatgctgatt ggtaaaatta gcaacccagc tacctcctga aacagcttta ctgtcaacta
    57901 ttttaacaat tccgacagca ttatcgcttc cttctttagg ataacctatt ccaattttat
    57961 ccttggaatc aacgtaatta ttgaccaccc cttttatttt atcaacctgt tctttttgtt
    58021 tatctgtcaa aatactataa aggtttggac cgttatatat ccaagctcct tgaactcggt
    58081 aaatttgatt atcatccaat tctgcaatag catactgtgc attcattgac cctaacgagt
    58141 gtccgtagac cgaaattttt gcgttaggat atttattcat aacttctttt agcagatgag
    58201 ctgattgttc taactgcttt gtaccttgtt tatgagatac actttgtgaa ccaaatttct
    58261 ttaataatag tttactagct attggaatat catttttaaa ccagtctgtt ttccaatcat
    58321 cccctccaaa cggtgctgtt gaccccttaa ataaaaccgt aacttcctta acatcctctg
    58381 gcttgtctaa agttccattt ggagtgatgg tataaatttg ttccccggtt tctttgcctc
    58441 catcttgaac gtgagcaact ttaccgatgg ggagtacctt atcttggctc attattttga
    58501 ctagtgtatc aagttggcta cctaattcat actcttgctt cgctatattt accctatcta
    58561 aatcagtcaa gactttttcc attaatgacc tctactttcc aataaatgag ttaactgggg
    58621 agattgatgg ctactttgac tgaccaaatg cccttgatta tccctatcca aattacaagt
    58681 aatatataag ttaggatctt tattaatgat taaccttacc gaaatccctc ccataggatt
    58741 ttttttgata ctttctttgt caatatcata cgacttaata acagcattct ctttgaaagc
    58801 atcttcatct tctgcataaa tccacttttg ataagcattt ttagcgtctt tgctattagc
    58861 tatttcccat aattcttgtt gtttattgtt ttcagtcact tttaaacctc ctacagtgaa
    58921 accaagccct aaaaggatta ttacaataaa actaagtaat tttattgact tcatctctcc
    58981 tccttttatc atataacact catttgatat ctagtttatc ataaatt
//
LOCUS       DATROY010000003        50168 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.003, whole genome shotgun sequence.
ACCESSION   DATROY010000003 DATROY010000000
VERSION     DATROY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50168
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(287..1006)
                     /locus_tag="VBD11_000122"
     CDS             complement(287..1006)
                     /locus_tag="VBD11_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6953102.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWRYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
     gene            complement(1008..1346)
                     /locus_tag="VBD11_000123"
     CDS             complement(1008..1346)
                     /locus_tag="VBD11_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6953103.1"
                     /translation="MNEFTTQIITVSVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISAEVGGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVK
                     RMNDEWKKGQ"
     gene            complement(1348..1644)
                     /locus_tag="VBD11_000124"
     CDS             complement(1348..1644)
                     /locus_tag="VBD11_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953104.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNNNKEK"
     gene            complement(1654..1815)
                     /locus_tag="VBD11_000125"
     CDS             complement(1654..1815)
                     /locus_tag="VBD11_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953105.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            complement(1878..2294)
                     /locus_tag="VBD11_000126"
     CDS             complement(1878..2294)
                     /locus_tag="VBD11_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6953106.1"
                     /translation="MKQWNIVGKPFPIYQDGVVVKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIRLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            complement(2305..2457)
                     /locus_tag="VBD11_000127"
     CDS             complement(2305..2457)
                     /locus_tag="VBD11_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953107.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            complement(2466..6293)
                     /locus_tag="VBD11_000128"
     CDS             complement(2466..6293)
                     /locus_tag="VBD11_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6953108.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFNGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWTDQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQEILDNAVFKANQALASADGKNTNYYGTEMPVDDPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKNLSDSAKKDATEALTNLISESKKLTDKMSALSKTEVEHYSTTNLQLTQLDG
                     TVKGLQTSYEALLKKDGDITQTLANYKQTIDQNSTSITANKKTVDGTLSSLQTQVTQT
                     ANEITTRLSQTNIEALITSKSAVIADNKVKETADSFSREITRVNNTIDNLKIGTVNLL
                     SGTKNWTKNWFNRNNWQSDDDNLTIGNFSLTVLKRKAMWSGISQLYAVKSGENYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVTSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEDLTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGWFMVGGF
                     MLSKGTADLPWSESKQDIKDNVTAINTIVAQTANSWAIKNLTSNGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLTDLNTGQIEFYTDNPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            complement(6306..7148)
                     /locus_tag="VBD11_000129"
     CDS             complement(6306..7148)
                     /locus_tag="VBD11_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6953109.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSHTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            complement(7161..11255)
                     /locus_tag="VBD11_000130"
     CDS             complement(7161..11255)
                     /locus_tag="VBD11_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO6953110.1"
                     /translation="MAGTFDGAIFADIGANTKDYEAAMARIVSTTQNAFRKAQDAAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNSFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIAQKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGAMVASGLSTASGIIGAFAATVSGLPAIFATAAARGVAIL
                     GAMTQAMGAVVSVAMAAIGPAAILGLVVAGLGLVNNQFGTQIDKLLNIAITRGSQIIT
                     NLVNGITSQIPQLIASGAELISKFANAITVLLPVIIQAGVQLISSLVQGVGQNATSLI
                     SSAIQIIGTFISSIASALPQLLAVGMELIVNLVQGIVQNIPLIIQYAQQIINNFGASL
                     QANMPSIVNNGIAILTNLVQGIIQMLPAVVQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGLQLVVQLAVAIIKGLPQ
                     IISAGIQLIIGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMSGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSAVSVTRSAMNSVVSAMNAAASG
                     ARSAGYNTGIGFANGLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYT
                     GEGFALGMQDWMDDINVIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDE
                     VRNQQLTSPTFEVHNELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            complement(11284..11514)
                     /locus_tag="VBD11_000131"
     CDS             complement(11284..11514)
                     /locus_tag="VBD11_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953111.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNISNDFAERVAKLNLHFQNL"
     gene            complement(11589..12044)
                     /locus_tag="VBD11_000132"
     CDS             complement(11589..12044)
                     /locus_tag="VBD11_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953112.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            complement(12095..12727)
                     /locus_tag="VBD11_000133"
     CDS             complement(12095..12727)
                     /locus_tag="VBD11_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6953113.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     TLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLKDREVYPAKFGYGKIDEIEYSEGVEDLVEASYALNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSSPGAVPEKLSEGH"
     gene            complement(12739..13098)
                     /locus_tag="VBD11_000134"
     CDS             complement(12739..13098)
                     /locus_tag="VBD11_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953114.1"
                     /translation="MIYSPSTLYLNEVKNRLGTLNIPIYFKLPKSDVLEPFIVIGSNV
                     SDTSKTAQTGAVVGDFGLNIDAFLPATSRTDAEEVKSRMIMSLGRNKRINAQLLMDNS
                     VGREVYHIAINITEILI"
     gene            complement(13102..13446)
                     /locus_tag="VBD11_000135"
     CDS             complement(13102..13446)
                     /locus_tag="VBD11_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6953115.1"
                     /translation="MSLTYRMRGLDRFLRSVERKQKSARIAVDKKLSKSAARIERQAK
                     ILAPVDTTWLRSQIYNEQQKLLHYRVVSPVLYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            complement(13443..13721)
                     /locus_tag="VBD11_000136"
     CDS             complement(13443..13721)
                     /locus_tag="VBD11_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953116.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            complement(13732..14088)
                     /locus_tag="VBD11_000137"
     CDS             complement(13732..14088)
                     /locus_tag="VBD11_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6953117.1"
                     /translation="MDKELLLVEVKIFKGIAKNDASQDELIKLAIDESIERILAKLNE
                     YSDEELLEIPRRLTFIARDVAIKRYNRLNSEGAKADSEEGRSFSWEDYLTEYDSILRN
                     VAYGKRCRGKGVARFI"
     gene            complement(14094..14990)
                     /locus_tag="VBD11_000138"
     CDS             complement(14094..14990)
                     /locus_tag="VBD11_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO6953118.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRREELT
                     SDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGAGLQKALSLSWAKLATFNEFE
                     GSQLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKVYST
                     AVDNLVFASLNVRGGDLGGLFADYTDETGLIAAARQRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEADKEVPGAVPGG"
     gene            complement(15009..15578)
                     /locus_tag="VBD11_000139"
     CDS             complement(15009..15578)
                     /locus_tag="VBD11_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6953119.1"
                     /translation="MAEENVATETTEQVDTQKEAVEQAKQEHERTFTRADISKMMTAE
                     RAKWEAEQSDAIEKARTEGERLAKMSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     QTLLVEKGLPLEFIDVVLGTTAEEVKTNIDNLQIIFDKAVEKRVNNRLTQKPPRTGNG
                     SVGMTKAEIMAVEDDDERMRLIAENRNLF"
     gene            complement(15721..15924)
                     /locus_tag="VBD11_000140"
     CDS             complement(15721..15924)
                     /locus_tag="VBD11_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953120.1"
                     /translation="MNKRIKKKRKLERAIVMLIAENAMQAEALRNQHKRIEQLEQIVE
                     HNAQATNNELDYIKNNYKKKWKK"
     gene            complement(15929..16117)
                     /locus_tag="VBD11_000141"
     CDS             complement(15929..16117)
                     /locus_tag="VBD11_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953121.1"
                     /translation="MGVRPPSFTTISIHQYSRIIKEEDMFIWRMVLVALGVLVLILII
                     GIAAIAVKSIIAEVKKEG"
     gene            complement(16145..17593)
                     /locus_tag="VBD11_000142"
     CDS             complement(16145..17593)
                     /locus_tag="VBD11_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEO6953122.1"
                     /translation="MLMDKKNQQQDHLDYWQKRQEDIVSYLDRADLDIFNELSRVYSN
                     QSLSLQKDLYDFYNRFAEDNKITYQDAVKKLRHEDLSDYAANANRYREQAEKDPELLK
                     RINEQYVSAQATRLDMLNLELIHRAGTMRGILDGLFSDHLRKAASYAYQKSVGGRSGT
                     INGPALEQLIKTPFDGYNYSEQLWGNVDNLVKDLQEAFKKGFVRGLGPREMARELGKR
                     YDVARHRAETLIRTDGTMIINNATAKRYIDAGIKYYRDLVQLDDRTTQICRSKARENK
                     KKLLSEIQPGINAAPYHFNCRTTIIPDEDELNTDVEPIADKSTKYFKDVTSDWIDGQE
                     YESQISMLNEYTKGGGTYRVDGHNVVLDHSNYEYRVANWLSKKTGLQVEMVPRVNFPE
                     NINTPDYLVDDVPFDLKEITGSGKNVIDGNLRKAKKQAPNIIFDITKTPLSFEEILGQ
                     LEHIYKIDRRGLNISIIKNKDEVLAVVEKERR"
     gene            complement(17553..19082)
                     /locus_tag="VBD11_000143"
     CDS             complement(17553..19082)
                     /locus_tag="VBD11_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6953123.1"
                     /translation="MFERKFLSGTRFNENANRQFIMLQEDFDAIEYNSQKWIDQLKKY
                     ISSFETEQLDRLKELKRYYLGDNNIKYRPAKTDEYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNEDSDIQKNINLMSVRNNEDYHNVAIKTDLSIYGRAYELLTVEEVDNGTEI
                     KLYQLPAEQTFVIYDDTYQHNSLMAVHFYDIDYGAGKRKQIVKVYTSNMIFTYEDFNQ
                     ETKGMSLKDEEEHYFSGVPVNEYSNNDDRAGAYESVLDNIDAYDLSQSELANFQQDSV
                     DAILVLSGNPYTGADENDFFEDGRINPNGRLGISIGFKKAKVIVLDDNPNPDGAKPDA
                     YYLKKEYDMAGSEAYKNRLVSDILRFTFTPDIQDLKFSGVQSGESMKYKLMASDNYRE
                     KQERLFKKGLMRRLRLAANIWSIKGNESTVYSQVNDTSIVFTPNVPQNDNEIVSAAQS
                     LYGIVSDQTIFEILNAVTGVDAEAELKRLKEEADEKQVMPEPRNVGDANGQEEPTAGP
                     S"
     gene            complement(19097..20377)
                     /locus_tag="VBD11_000144"
     CDS             complement(19097..20377)
                     /locus_tag="VBD11_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6953124.1"
                     /translation="MTTKQRPKINIVIKYPSKVFNKHIYDKLYNYDNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAN
                     RNEAYYKIYALGQFATLEKLIFPKYEKRILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKSLGYAKEEIRGDSAEKKSNQELRNLGIQ
                     RMIDVKKGAGSVMQGIQYMLQYDIVVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSSDRNKRLNNAKYYFR"
     gene            complement(20367..20798)
                     /locus_tag="VBD11_000145"
     CDS             complement(20367..20798)
                     /locus_tag="VBD11_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6953125.1"
                     /translation="MSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARSIGQENLT
                     KPDIKAYIDERIEKLESAKIATQEEVLQYLTSIMRGDQQEKTLISIGELGQEIVDIDV
                     GAKDRIKAAELLGKRYRLFTDKIEMDISSDVTISVGEWDDD"
     gene            complement(20919..21332)
                     /locus_tag="VBD11_000146"
     CDS             complement(20919..21332)
                     /locus_tag="VBD11_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6953126.1"
                     /translation="MSKLSNAQLKAFDEWLFDYRDIERKIAIRKLELQTPVSTDINVG
                     GGKSNLVAKETEDIVIKWSKDGKIKSYENFRESVDRVKALLDDELNYIFDLRWGTGSN
                     NTWEEIAYKIHTSRAGTYRKRDRILTLFAQQIGKL"
     gene            complement(21410..21742)
                     /locus_tag="VBD11_000147"
     CDS             complement(21410..21742)
                     /locus_tag="VBD11_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953127.1"
                     /translation="MSLIDEVKECGSESHKKWFDRYFDGLKIEEKLKQSAMKGFSGYK
                     ISIPKNDEYLARRLDSQKTIDLLKYRLGDGFDVEIKNLESSYKIFGKPVVLSRDLFIF
                     WRTGDKNG"
     gene            complement(21745..21975)
                     /locus_tag="VBD11_000148"
     CDS             complement(21745..21975)
                     /locus_tag="VBD11_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953128.1"
                     /translation="MNEQNNKLHKQSIVIVVLTVAVSVLLTTNIALYNYYQPQITGLQ
                     QQLIRTQYRLKESSEQNQRQTKRIAELTGNGG"
     gene            complement(21972..22208)
                     /locus_tag="VBD11_000149"
     CDS             complement(21972..22208)
                     /locus_tag="VBD11_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO6953129.1"
                     /translation="MTGELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLVEYWLLESVNE"
     gene            complement(22205..22420)
                     /locus_tag="VBD11_000150"
     CDS             complement(22205..22420)
                     /locus_tag="VBD11_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953130.1"
                     /translation="MKIDYIDFFERVVPGWMRESNKKMQELGFGTEQYWVWVNMSIVD
                     ICESYGNDDLVNGQFHMIWEWLERKTK"
     gene            complement(22417..22815)
                     /locus_tag="VBD11_000151"
     CDS             complement(22417..22815)
                     /locus_tag="VBD11_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO6953131.1"
                     /translation="MTIEFFIPMKKIPTTTHQQKKVRVVNGKPQFYEPDSLKEARAMF
                     MDKFAQHVPKEKLNGPIRLTTKWLFPKIKKSVNGQYKDTKPDTDNLNKLPKDCMTELG
                     FWNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            complement(22842..23039)
                     /locus_tag="VBD11_000152"
     CDS             complement(22842..23039)
                     /locus_tag="VBD11_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953132.1"
                     /translation="MKEYKKRMIVKYQELKSRAEKLSVLINKFYIDSLDFKLQCPIEL
                     LVAQWHAMGAYLKILEQRAAI"
     gene            complement(23409..25688)
                     /locus_tag="VBD11_000153"
     CDS             complement(23409..25688)
                     /locus_tag="VBD11_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6953133.1"
                     /translation="MAENDFNLLPLLDYINPATVDYQTWVQVGMALKHEGYTAMDWDV
                     WSQSDSRYKKGECFAKWDSFQGNGFGTITGATITQLAKDNGWTSSEYRNSDDAHELSW
                     DDTIDRDYKIVDKNWIESKEIQEPRNWNPVQDLITYIDTLFESTDKVGYVTETYPITL
                     DTGEIVYKPTKGAYDRTAGQLIESLQKNPTDLGAVFGDFKEEAGAWIRFNPLDGNGVK
                     NDNVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDARDYQEY
                     RKRVDYIYQICKKNGLDIDTQNRNPSRLSRMPGVTRNGHKQFLIDTNVGKTNYEEWYQ
                     WIEDLNDDLPDPETLADEWDNMPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELS
                     IALAEGKEWLGWQCEQGKVLYVNLELDRPSALHRFRDVYEAMSLPPANIKNIDIWNLR
                     GKTVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATEL
                     GCSVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTEELIKSRSEKAA
                     AKIYQQALQEKALAYYQQEVTLDDLESRYQMQQHFDKAIKDIMIKQPYLEAVKKAQYE
                     VEISTAWRVEGTLREFAKFQPVNMWFSYPVHDVDTTGVLADISLEDNVPTWKKNFEKK
                     SPRESREKKSQKVETAINSLNDGIEPVTIDNLIEYFSTEDKPVSEKTIRRWIKENGKF
                     EVKNKQILPIEEPKTNKSL"
     gene            25759..26010
                     /locus_tag="VBD11_000154"
     CDS             25759..26010
                     /locus_tag="VBD11_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953134.1"
                     /translation="MPEIKITFSDGSIVIFHEEQSFQSVVKSNDAMSLSKIYSLWNHV
                     HDGLIPSFLELIANSQFFFDLENPQIYYASNAVIKIEAI"
     gene            complement(26003..26200)
                     /locus_tag="VBD11_000155"
     CDS             complement(26003..26200)
                     /locus_tag="VBD11_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953135.1"
                     /translation="MENINEQIEKFISNFADEAIEKSETYSEAILYVDKHSSLTEFGQ
                     VVKKAIQEKIRDIALNSRIIK"
     gene            complement(26209..27792)
                     /locus_tag="VBD11_000156"
     CDS             complement(26209..27792)
                     /locus_tag="VBD11_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6953136.1"
                     /translation="MKLREYQEEARSAVQQEWEQGKKKTLLVLPTGCGKTIVFSKIIE
                     DRVKLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAESTSLGSWYRVVVGSVQTL
                     QREKRLSKFPTDYFDTIVVDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLTLDLSTVSQQAGDFKASEIGTALDPYL
                     EQIADEMVKQCSNRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKEKLLLLD
                     FLWHTERHELCRPAHLIAKTPEVAQKMVENMTDETEVAFELLEAEEVASKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSINAEDLVDYVPEFGWEMAPASEKQLKTLEKFGI
                     FTDEIGNAGKAAKLLDRLDKRRQSGLTTPKQIRMLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            complement(27798..28406)
                     /locus_tag="VBD11_000157"
     CDS             complement(27798..28406)
                     /locus_tag="VBD11_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953137.1"
                     /translation="MGKAKDYTGQRFGRLVVIERLPQDPYTKAKYKCQCDCGKLTIVN
                     SSNLATGHSTSCGCIVTKHGFARKERLYNIWVGMRQRCRDKNSNDYIKYGGRGITICD
                     EWEEYIQFKQWASSNGYRDNLSIDRIDVNGNYEPSNCRWANSTEQANNQRNNHLITFK
                     GKTQTIKQWSSELGISNITLLTRLNRGWSITKALTTPTQKGV"
     gene            complement(28406..29011)
                     /locus_tag="VBD11_000158"
     CDS             complement(28406..29011)
                     /locus_tag="VBD11_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953138.1"
                     /translation="MTEYNNNFEREFGWDDTIQEDAKEFITLQPGDYVFTVTDFERAR
                     HTPNPQKTGKLPACNKAVISLEIETDEGVASLKHNLFLHSSTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGATGVCSVKNRTYNDNVYNEVKSMIYADSVDWTKVLNANTQPQAPQ
                     PTYQQPAQQTTNFAPQQQPAPQSYQQGQMQASQQQGGWGGF"
     gene            complement(29107..30180)
                     /locus_tag="VBD11_000159"
     CDS             complement(29107..30180)
                     /locus_tag="VBD11_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6953139.1"
                     /translation="MQITRGKRARAQRVIIYGPEGIGKSSFAAQFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAFVKANPTCCKTLVIDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNMLQELIELGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTADNNKKKATGGTRVLYTQHHPAWDAK
                     NRHGLQEEMPLDYAGIAHIFAQAPSQPVAETPPVQQPVPTQPIHEPVHEQQAQMSGPT
                     AATEYPSVLPKALTDLMTPEQVTPDELVAVANIRGHFPPLTPIENFPPDYWNMIVANW
                     GATLEVIKTQVRATEQPFTVEGS"
     gene            complement(30195..31514)
                     /locus_tag="VBD11_000160"
     CDS             complement(30195..31514)
                     /locus_tag="VBD11_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6953140.1"
                     /translation="MTITINKLEIENVKRIKAVKVEPSSKGLTIIGGNNNQGKTSVLD
                     SIAWVLGGNKFKPSQAAREGTMVPPTLKITMSNGLIVERKGKNSSLKVIDPNGQKGGQ
                     QLLDSFVEELAINLPKFMDGTPKEKADVLLEIIGVGDQLAELELKEKELYNQRHTIGV
                     IADQKEKFAKEQPYYNDAPKELISIADLIQQQQEVLAKNGENARKRQNLSVIQQNHQF
                     KQSEVEHLKQQLATAEKQLQELSSDLEIAQRDTMSLIDESTAEIEENISNIEEVNRKV
                     RANLDKDKAEEDAKHQREQYNILTNDIESIRQQKRDLLINADLPLEGLSVDDGKLLYL
                     GQEWDNMSGSQQLIVATAIVRKLKPDCGFVLIDKLEQMDNITLEQFGKWLEQEGLQAI
                     ATRVSTGEECAIIIEDGYSVENKAHSFKTAEGGSFAETVAPTWQGGF"
     gene            complement(31498..31728)
                     /locus_tag="VBD11_000161"
     CDS             complement(31498..31728)
                     /locus_tag="VBD11_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953141.1"
                     /translation="MIEVERQPKNTYSRIRCSNNIYDEIANIANECDLTLKEVTDALL
                     NYGLANAQIISSEKVVTESKLVIGDFDNDNHN"
     gene            complement(31721..31948)
                     /locus_tag="VBD11_000162"
     CDS             complement(31721..31948)
                     /locus_tag="VBD11_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953142.1"
                     /translation="MLKEVLLENELLRDENRRLTNEMADYYFTNVAKANLLDIIVNEG
                     YILDSTLEKCIAKLDNVDRQTLEKNWSDDND"
     gene            complement(31942..32091)
                     /locus_tag="VBD11_000163"
     CDS             complement(31942..32091)
                     /locus_tag="VBD11_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953143.1"
                     /translation="MKKLFKKLFVKKNQVIETRQKWTIETHGWEANARRYDNMIERNN
                     RGRTC"
     gene            complement(32096..32407)
                     /locus_tag="VBD11_000164"
     CDS             complement(32096..32407)
                     /locus_tag="VBD11_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6953144.1"
                     /translation="MPKAELVYRPAKQSEKADYGDYVHLCQIWEGLTVGTAKVWAAEM
                     RNHPDFKQFINNPTHRIVFINYEGFKLFVTWKSRNRYKPKKETLAEMLENIKKERQLG
                     V"
     gene            complement(32487..32678)
                     /locus_tag="VBD11_000165"
     CDS             complement(32487..32678)
                     /locus_tag="VBD11_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6953145.1"
                     /translation="MPDIAVGREKVVTFLKSNNIKKSDLAAAYGLNRQEVTNILSGST
                     RGPKANQFILRVIADYSIE"
     gene            32729..33517
                     /locus_tag="VBD11_000166"
     CDS             32729..33517
                     /locus_tag="VBD11_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953146.1"
                     /translation="MSRYSDYKYANTILKSIEERPEKYLIIHYSCESFYNLGSKSPRI
                     ASISVRQFNNAQTNNFSIHQYSEILDTPITDDTYETIEKELLTDFFTFVDRNTDKNWI
                     HWNMRDTVFGFHAIEQRFKVLGGTPIIIDDDKKIDLAYLFKKMYGGGYIDNPHIEKLL
                     ELNNLTPHRFLTGKQEADAFSSRQYHELSMSTSSKVNSFSTFLTLAINDDLKTNTPKW
                     KMRGTNLSGLLSAFQETTSGKVIIGIINLIVGGIVGAIIAKYIQ"
     gene            complement(33468..33713)
                     /locus_tag="VBD11_000167"
     CDS             complement(33468..33713)
                     /locus_tag="VBD11_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953147.1"
                     /translation="MKKNIVDLQKRNEHFQWVADSLEGKENELYVERDWYDNPTLISK
                     EDAKKEVEQVQQKLILLQKKSFIEYILQLLHQLFRRQ"
     gene            34009..34356
                     /locus_tag="VBD11_000168"
     CDS             34009..34356
                     /locus_tag="VBD11_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6953148.1"
                     /translation="MFPTFEKIKELCKQRGITLIQLEETLGYSRNTLYKLKTQKPNAE
                     RIAEIADYFNVSTDYLLGRTDNPNIAKDSQEYTSDDLRKMAEKAKTFDGKPLTESDID
                     AIQNIIEIYLKGR"
     gene            34360..34746
                     /locus_tag="VBD11_000169"
     CDS             34360..34746
                     /locus_tag="VBD11_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6953149.1"
                     /translation="MTIEEIVDCHKVNLAYFDNELWPRPGIYIDDIKVIFVNKSLSLD
                     AQKRVILHELGHCERNIGDYKTLREKHELEADRYMISHLIAEALSQLEDKREFNYLQF
                     MQYHQLTTTADECMVIDEYYNLLKVV"
     gene            34764..35315
                     /locus_tag="VBD11_000170"
     CDS             34764..35315
                     /locus_tag="VBD11_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953150.1"
                     /translation="MDINKIKEFAVSTSEKAKDGIIKANEMRKRASQESKIVLPPTIN
                     LGFNTPTAIRKTVEGDYYIGFYSEKPDLFKFVDFQFDGSRIIEKTVTKGKTTQKGRSG
                     STIIGGLIGATINPTAAVLGGLAGANRGKKGTINSTSTTITEEIPGKAQLTLRNLKTN
                     DIKIIKTKLTQAQANNIQNFFSK"
     gene            35430..36572
                     /locus_tag="VBD11_000171"
     CDS             35430..36572
                     /locus_tag="VBD11_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6953151.1"
                     /translation="MWSEKHKSGKVNFVERYKDPYTNKWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTNLIDEWWIFYQQEIKRSSIVTLKGNIREIRAEFGIN
                     IPVVNIDPRYVQNYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPKI
                     KKTLNDWKKIEEKYLEEEEIKRLLKELFRRPSTHRLGFLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYINGEKTSPKTLASYRETIMSKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTRNGVPIQINSFNLALKKANERLEQPINKNITSHIFRHTLVS
                     RLAENNVPLKAIMDRVGHADAKTTVQIYTHITKKMKSNIADIMENY"
     gene            complement(36660..36935)
                     /locus_tag="VBD11_000172"
     CDS             complement(36660..36935)
                     /locus_tag="VBD11_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6953152.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(37038..37640)
                     /locus_tag="VBD11_000173"
     CDS             complement(37038..37640)
                     /locus_tag="VBD11_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO6953153.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            complement(37615..38454)
                     /locus_tag="VBD11_000174"
     CDS             complement(37615..38454)
                     /locus_tag="VBD11_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6953154.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(38447..39283)
                     /locus_tag="VBD11_000175"
     CDS             complement(38447..39283)
                     /locus_tag="VBD11_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6953155.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(39396..41054)
                     /gene="recN"
                     /locus_tag="VBD11_000176"
     CDS             complement(39396..41054)
                     /gene="recN"
                     /locus_tag="VBD11_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6953156.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(41066..41539)
                     /locus_tag="VBD11_000177"
     CDS             complement(41066..41539)
                     /locus_tag="VBD11_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6953157.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(41526..42353)
                     /locus_tag="VBD11_000178"
     CDS             complement(41526..42353)
                     /locus_tag="VBD11_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO6953158.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(42346..43218)
                     /locus_tag="VBD11_000179"
     CDS             complement(42346..43218)
                     /locus_tag="VBD11_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6953159.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(43218..43433)
                     /locus_tag="VBD11_000180"
     CDS             complement(43218..43433)
                     /locus_tag="VBD11_000180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6953160.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(43411..44751)
                     /locus_tag="VBD11_000181"
     CDS             complement(43411..44751)
                     /locus_tag="VBD11_000181"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6953161.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(44877..45713)
                     /locus_tag="VBD11_000182"
     CDS             complement(44877..45713)
                     /locus_tag="VBD11_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6953162.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(45710..46564)
                     /locus_tag="VBD11_000183"
     CDS             complement(45710..46564)
                     /locus_tag="VBD11_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6953163.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(46703..48397)
                     /locus_tag="VBD11_000184"
     CDS             complement(46703..48397)
                     /locus_tag="VBD11_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6953164.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(48684..49418)
                     /locus_tag="VBD11_000185"
     CDS             complement(48684..49418)
                     /locus_tag="VBD11_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6953165.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(49411..50103)
                     /locus_tag="VBD11_000186"
     CDS             complement(49411..50103)
                     /locus_tag="VBD11_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6953166.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
BASE COUNT        14727 a        10285 c         7874 g        17282 t
ORIGIN      
        1 cgtatgcgag ggaggccatt gatatttata tatacattat atatttttat atagcttatt
       61 gtcaatgatt ttccctttat ataattaaac tttttaataa aatatatttt tcatccagta
      121 aaaggcacct aaatacctcc tgaaaaatct gttcaaaata ataaacagcc cccgcaaaag
      181 cgagggcatt tgtcttatct aaaggaattt tacctcctaa attgtttttt tagttgcggt
      241 gtaacttaca ccattaacag accgacattt atatcgctct gttggtttac atatctgttg
      301 catcaattaa gtaagcatct tctacccact gattagactg tggagcgcct atccttgccc
      361 agccttttac tttttcgtaa actcgaactc tagtaccagc aacaagcaac tccttatcag
      421 tgctattgac gtcaggcttg gactcaacgt aataatcttc tgaaattgtt gcttcgtaat
      481 atggcatatt tgaattactt agcggtgtgt taacgtctaa ctctttttca aatttagata
      541 caactgattt attatcaacc ttagcatttg actgtttccc agagtaacga taagcgtaaa
      601 catacggttg accattatat ctccagattt catcatggtt attcactgta attgagttat
      661 aaccataatt acagtgaata atattgtctg gatcaacaaa cataccagta tgtccaaaag
      721 ctccagccga agccccacgt ttaccccaaa taaaaatatc acctctttgc gcattccaat
      781 tagtattttc tgcaataaga acataaccgt tttttatcaa ccaatcgtgc tcatattctg
      841 tatttactgc ccagccatta tctgatgcgc ctgctgagcg tagagcaaaa tagacagagc
      901 tagagcaatc gtaagatgaa ggaccatttc gatagtccat cgagtaagta actttacctt
      961 ttctagatgc catccatgcg atagcttgct caatattaat taccatatta ttgacctttc
     1021 ttccactcat cattcatgcg tttaaccgca gcttcgataa aagtttcaag ttgtgtctgt
     1081 gtaagattaa tattatattg cgataaacca tcaataacag cggttttagc ttctgttagc
     1141 ttatcttgtc ccttgatacc accaacttcc gcagatattt gttcaactgc ctcaacagca
     1201 ttacgagcga caatttctgc gattttaaca gctttttctc cacctttttt tataagtaat
     1261 ttttttactt cgtgcgtgag aatacctgcg atgataccaa aaattggtac tgatactgta
     1321 atgatttgtg ttgtaaattc gttcatgtta tttctcctta ttattattaa ataatgtttt
     1381 gatttgctct ttattgatga tgatatcatc ctcgacacga cctaaacgct cctcgtgacg
     1441 atcaataaca gcttttgtaa tctctcggtc tctatctata tttttgagct cgtatgctag
     1501 ttctttgagt gagtccttga gctgagccat agcaatctcg ttagcttcca ttgctttttt
     1561 aaagggatta acaataaatc cccatactcc caaaatagat aaagcagcac ctgcaaaagc
     1621 gccaacttgc acaaaatcta tcattaagtt acctcactct gattctttat cagtaacttt
     1681 ttgactaagt aactcaagca caacatctcg taaattaaat aatttaggta ctgcttcgaa
     1741 atcacaaata ccattcgcaa cacgacggta ccaaacgtca acaataagat gatctttttt
     1801 aaaagtatat gtcatattaa attctccttt ttttattaaa taatatagtg ttataacggt
     1861 attcaacgtt aattccatta gcttgattcc atcgatgtag acggcacgga aaggctagat
     1921 acacttgatg tcactaagtc agttaactcg acaactgctt tatcaacttt aagctgcata
     1981 tcttttgttg cttgcgacat cttagaaatt gcctcatcag cctctgcaat ttttttgatt
     2041 aaagttactt tgtcagcttt tagttgctca atatctttta cagactcaag cattgcgaaa
     2101 tcaacgacat tttcttttgc aaaagtttca agtacaagtc ttatcagtga ttgattatcc
     2161 ttatcaacat gattaccaat tagctcttgt ggtaaatatg taccatcgtc gccttgtaag
     2221 cgtacatccg tttttacaac cactccatct tgatagattg gaaatggttt tccaacaatg
     2281 ttccattgtt tcatgttact cacctcactt tgctcctggc ttagttatct catctaattg
     2341 ctgatttaac tcagcaattt gtaattgtaa ttgctcattt tgagcttgta gagttgcttt
     2401 atctaaagat aattgcgcta tttgtaaagc taaatttgat tttatttttt cttccatttt
     2461 ttctcctaaa tctttcctgg aatagtataa ctgtagcctt tttcagtcgt tttattattg
     2521 tgtagtagct gcaagttatc aataatcata ttaattgtat ctttcatgct tgtataagtt
     2581 gatgtatttt tccatagcca aatatctcct acttttatct tagacgttac acgatgattt
     2641 atgttacgtg catctattga tagctgattt ggcaaagtaa tgatttccca accatcagcg
     2701 ttagtgtacg cagagcttgc taagtgaata ctatcgccaa ctaaatcaag agtatcgatg
     2761 tctttaccat tccaagcgcg aatacctacg aaaccaccgt cgtttgacga ttcacttccc
     2821 catcgattag aacctataac cgttacgcca gccgcgccct ttccttcaac attacctgtt
     2881 gcaaatttaa cgaattggtt aggataacct gcagtaatac gtttgatagc tggattatct
     2941 gtgtaaaatt caatttgtcc tgtattaaga tcggttaaca ttgcaccgtt ttgcgatatt
     3001 aaaacgccgc tacgtatcag gtccgctgac attcttccgc tggtaatatt actagcatct
     3061 aaattaatga catttatacg attagcatca attgttcctg ctgtaatctt atctgcagtt
     3121 aaatctttta tcatcgcatt agtaataatg ccattatcaa taatagattg acctgacaaa
     3181 tgtattaact taccttccag ttttacgtta ccatcagtca aattaagctg actcaataca
     3241 gtaccgttag aagtcaaatt tttaatagcc caactgttcg ctgtttgtgc gactattgta
     3301 ttaatcgcag taacattatc tttgatatct tgcttggatt cagaccaggg tagatcagca
     3361 gtacctttag ataacataaa accaccaacc ataaaccagc ctggatcact ggccaacatt
     3421 gcgaaccgtg gtctgatttt accgtctcga gttggaataa atgttatttt aaatcgtcta
     3481 atggtttgag tgacgtcttt aataatagtt tcacgaggag ttgtgcttgt aatatgatga
     3541 ttccaaagat catacaagta gaaatataga tttcctgctt gttctctaga aatgtatgca
     3601 gtaaacgtat actcttgatc ttttttgaca tcatattcga ttaaaggtga caccttatta
     3661 ccgcttaccc attttttaaa aacaaatggg taaggcgatt gtgttagatc ctctagtact
     3721 gcaccttctg tttgccatcc tgtgaatttt tttgtgccag gtaaaatgtt gttatcaagc
     3781 tgactaactg ttgcaactat accgtcagcc gtttgggtaa ctcgtgacaa gcgtccatcc
     3841 acattagata ctgttgattg tatattatca actttttgta ttgtagctgt cagactctcg
     3901 ttttgtttac caatcagttg actatgacta ttaactgtat cgttgatttt attaaattca
     3961 acagtcattt tatcttcttg atccgctggg gactctgaat agtcacttgt aacatttcca
     4021 tgttcaagct ggtaacctgc aatatacaac caaccatttt cattaaagcg ttcgaagcgt
     4081 ggtaaaatat agccatcaga agttacgtta aatttaataa cgatacgttg ccaatccgtg
     4141 tttaaagtga tcgacttgaa actaatactt atcttagcct gttgcgattg atcatttggt
     4201 aacaaatata aattaacaac atcattatct atgcttgatt tagcatatgc actaaaaacg
     4261 taattctctc cgctttttac agcatacaat tgtgatatac cactccacat tgctttgcgc
     4321 tttagcactg ttaagctaaa attaccaata gtcaagttat cgtcatcaga ttgccagtta
     4381 ttacgattaa accaattttt agtccaattt ttagtaccag atagtagatt aactgtacct
     4441 atctttaaat tatcaatagt attattaaca cgagttattt ctctgctaaa actatctgca
     4501 gtttccttga ctttattatc agcgataaca gcagacttac ttgtaatcaa cgcttcaata
     4561 tttgtttgag ataatctcgt tgtaatttca ttagccgttt gtgtgacttg ggtctgtaga
     4621 ctgctaagcg taccatcaac agtctttttg ttagcagtaa tacttgtact attttgatca
     4681 atcgtctgct tataattagc gagtgtttga gtgatatctc catctttttt taacaatgcc
     4741 tcgtagctag tttgtaatcc tttgactgta ccatcaagtt gtgtcagttg cagattagtt
     4801 gttgaataat gctcaacttc tgttttagat aacgcagaca ttttatccgt cagtttttta
     4861 gattcactaa taaggtttgt caatgcttct gtagcatctt ttttggcaga atcagacaaa
     4921 ttctttgctt gattaattaa ttcgtcttgg ctaacatttt tagcaacaat tgcattatat
     4981 ttttcagtta actgttcatc agcaagtttc attgctgcat taacctctat gattttatta
     5041 gtaatttctt tctttacaaa atcaatatcg tcgctaaaag aattgataat ccattcagca
     5101 ccattccaga aatacattct ggtagtatcg ccaacagtta aaaaaagcaa atcacccttt
     5161 cttagcgtgc cttttggatc atctactggc atctccgtac catagtagtt agtgttttta
     5221 ccatcagctg atgctagtgc ttgatttgct ttaaatactg cattatctag tatttcttgg
     5281 ttattattaa tcttacttga taattggcta acagctacag tttgtttttt gattgtacca
     5341 atatcgttac aagtgacttt atggttaatc agctgacctg ttacatcata ctcactttca
     5401 aacgatacta ttcggatttt ttccttaaat cctagtgtct cattgatagc cataatataa
     5461 tcaccggcaa taggttgagt gtactgataa cctgcttttg taagatcttc aatgtcaatt
     5521 tttacagcga tattgtaaga cgaatctaca ttttgtttta gtactgattt taaattgtca
     5581 gcgttagtgt accgctcatc aacaacaggc tcaccctcaa ttcgtccata ttcctttgca
     5641 agtggacttt catattctac ttcgagcctt cctttggaat gatcctcttg gtcagtccac
     5701 gctccaaaac ccttctgata tgtaataaaa tcattgattt tcttttcaat tatgatttca
     5761 ttcatattga aatttttacg tacgattgtt gataaatctg ttcctgtttt ttgtagaatt
     5821 cgaacaacct tgccacgaac aaaaaattca actcctgcac tcttaatgat gtcattaaat
     5881 aaatcaagtc gcgatttata tccaaacgat tgcttttcga aagatttgac aactacttct
     5941 aaactgtaag tatacccgct gccattaaaa ataaagttca aatatgcttg gaaagtatgt
     6001 gaaccatcat ttaattgctt atgtagtgat gatttatcaa aatcataaaa aaattgatga
     6061 actgcatcaa attcaacttg aattttgtta ccagtatcag taggtttagc ataaataatc
     6121 ttgtaatact catcatcaaa ctcaagtttc caacctttat cgaccttgtt tagtacctct
     6181 tcgttagtat aaattgttcc tgataaagac ctttctccgt taactgcatt agttgtttta
     6241 acaacagctt gagcttcaaa ctcattatct ttatgatcta aaaaagttgt caatctatca
     6301 cttccttact tgtaaagttc tttaaagttt aaaaacttga ttgtgccttg aaaatttgtg
     6361 ctgtagagaa tagtcttgtt gactttttgc tttaaaacaa aatattcatg attggttcta
     6421 gcagtcacat ttatcttatt caatgttgtg gatataccag tgattacaat aacgtcgcct
     6481 tgattaagtg gtgcactata ttgataagcc cagcgcctcc catcgatttc aagataaaaa
     6541 ctactttgtc caccagttga agttaactct acagaccatg ggtattctaa ctggctaaaa
     6601 ggtgcagtac cagaataatt aaacgttccg ttagtaatga ctatattttg tgcaatagtt
     6661 tcaccaaatg gaagttctgc agtctcaaat gcaattgtaa agttgtattt gagaccttca
     6721 ctagacttac caacaaaagt gtactcaggt tcacttgaga cataaacttt ccaacgatag
     6781 tgccacgctg tatgagattg atttaataag tctaaatcac ctgctttcat cccaggcaat
     6841 tcgaaatcat ataaattttc tttctgagga tacattttag taatgtaaaa aggctcacta
     6901 tccactagta agccattcac atcatctttt tttgtcataa aatctttaat agaagctaca
     6961 actaagcgcc cattaacctt aacttttttt gtgccatact tagcactagc aaagatttta
     7021 ccgcttcgac ctttcactgt tcgactatca acctcaagag atgatgaacg gtcatcgaca
     7081 tcaagtattc gtagaccaat ctctgaaaat cgaaaggatt gatcactctt ttgtactaat
     7141 aaatccattt ttctcctctc ttatgtcgca aagttgaagt aattatcttt attactttct
     7201 cttgcttctt tagatttaat agttgtataa atttcatcac caacaagttc attatgaacc
     7261 tcgaacgttg gactagtcaa ttgttggttg cgtacctcgt cgcttagatt atcaagtgat
     7321 gatgatacac ctgcacttga tatgtttgca gatgtcgcta attgtgaatc tgtttcccat
     7381 cgctgatcag tcacagcatt agcgtactct ttacctatta cattgatatc atccatccaa
     7441 tcctgcatac caagtgcaaa accttcacca gtataagcac caagttctcg cgttactcgc
     7501 gaaggcgaat gaatatccat tgcgcttctg attgttgcag cgacattagc agcaatacta
     7561 ttagcaatag ccataatagt tcctgcagtg cttgctaggc catttgcaaa tccaatccca
     7621 gtattataac ctgcagatct agcaccactc gctgctgcat tcattgcaga tacaaccgaa
     7681 ttcattgcac ttcttgttac actcacagcg ctgttcattc cactagtaac agtgctgttc
     7741 attcctgaca ttgaacttga aactgtagat ttagcttgtg taaaactgct attcacactg
     7801 ttagatatat ttgcaccgcc tgattttgca gcatttgcag cttgattcat ccctgtagat
     7861 acagtgctag ttatacctga catgttatta gctacaccac tagccatacc agctgtagca
     7921 ccagtaactc cagaactcat tccattcgcg gaagtggtag catttgcttg tgcagtttgc
     7981 atattacttg ttgcctgttg cgataattcc aagaaattcg gtagagaccc cagactcatt
     8041 tggctagtag cgttattaac attagcagtc atttgattag cttgattagt agcatttgta
     8101 tttgctaaag tcatgttcga actcaacgct tcattaaatc ctgccgcatt cagcaaacct
     8161 tgatttgcca tagatgctgt ttgtgcattt acatttgttg ccatctgagt agcatttgtt
     8221 gtcgcattta aattagcaag accagtattt tgactaatgc tatttagcat agtagttgtg
     8281 tctatgttag tttgattagc cattgtggtc gtctgcatac caacattcat agtcattgtt
     8341 gacatgtctg atgtaacttt agcggcactt gttgagctct ttcctgtgat ttggtcccac
     8401 atagaactaa atccaccttt aatgccttcc catacccctg aaagagcatt cggaatagcc
     8461 tcaagcatag ctttgccaag tcctataatc aattgaatac cagctgaaat aatctgtggc
     8521 aaccctttaa taattgcaac agccaactgc acaactaact gtagccctgc agatataatt
     8581 tgtggtaatg cttgtgataa accagatatc aatgactgaa taatctgcat cgctgcttgg
     8641 attacctgtg gtaaattttg aataatacct tgcactaaca tgattataat ctggatgccg
     8701 ccttggatta cctgtggcaa atactgagct ataccggaaa taaaacctgt gatgacttgg
     8761 gttgcaattt gtacaactgc gggtaacatt tggataatgc cttgcactaa attagttaag
     8821 atagcaatgc cattattaac aatgcttggc atgtttgctt gtaagcttgc cccaaaattg
     8881 ttaataattt gttgagcata ttgaataatt agtggaatat tttgcacaat tccttgaact
     8941 aaattaacta tcaattccat accaactgct aaaagctgag gtaatgcact tgcaattgaa
     9001 ctaataaatg tccctataat ttgtattgct gaactaatta aactcgtagc attttgtcca
     9061 acaccttgaa ctaaactaga tattaattgg acacctgctt gaataataac aggtaacaat
     9121 acagtaatag catttgcaaa tttagatata agttcagcac cactagcaat taattgaggt
     9181 atttgtgatg taatgccatt taccaaatta gtgataattt gagaacctct agtaattgct
     9241 atatttagca atttatcaat ttgagtacca aattgattgt ttactaaacc aagacctgct
     9301 acgaccaatc ctaaaattgc cgctggccct attgctgcca tagcaacgct taccacagct
     9361 cccatggctt gtgtcattgc accgagtata gcaacacctc tcgctgccgc agttgcaaaa
     9421 atggcaggta aaccacttac tgttgctgca aaagcaccaa ttattccgct agcagttgac
     9481 aatccactgg ctaccattgc tccgaatgat cccaacgctg aaccaacagt cccaagagca
     9541 acaccaatct taccaataat tgatattaaa ggcataatag ttgcaatagc acttgcagga
     9601 tttaataagg cccaagctgc tattgcagta ggacctatag atgctatctt tgatttgatt
     9661 ctatcaatag ttgctgacga tacttcacca gtttttgcaa attcacctaa agcagtattt
     9721 aataatccaa aagctgattt aattccacta gttagtggac taaagtcaat attcaccatc
     9781 ttggagattc tagcgaatgc tgcaacaagg tttggcatta ttgagttcac agttgcaaat
     9841 gcagaattta tagctggctt aatactatta atggattctg caatggtctt cataccatta
     9901 gctttggcag cttcatcaaa ggctgaaatc atacctgcta cgccttttac gacggcggta
     9961 tgtacgttct tccatgcagt tcggattcca ccagccgatg attgggccat ctcagcaaaa
    10021 ccaccctgcg ccttgtttag ttcaatcatt ttatcagcga attcttgggc agtaatactg
    10081 ccgtctgaaa gagctgcttt taagtcctga acaccatttt taccaaatcc gaatgactct
    10141 gccattttag acattaagcc aggggcagct tcagacacag aattaaactc ttcggcatat
    10201 atcttaccag accccaacga ctggttaaac tgtctaagtg cttgctcagc cccttcagta
    10261 gtcgctccat aaccaatcat tgcattatta aatgctaatg ctagctctgt tcctttatct
    10321 aagctacctg tagttatagc taattgttgg gcacttttaa cagcactgtc tagtggtgta
    10381 ggaagtcctt caattccttt agataattta tctattgatg ctttagactg ttgtgcagaa
    10441 tacccaaata gcgccatcgt tttcggaaag cggttcatgg tatctacacg acttattgca
    10501 ccgtccattg ctcctgtgat tgcttggatt cctttctgag caatggccat caaactaaaa
    10561 gcagaaatga tacttcccac agtgcttctt agtttttcac cagcagatga tgcgcttgcg
    10621 aatctctgac taataccatt tagggcacta gttgcagaac tggacatcct gctaaaacta
    10681 ttgctaagac ctgtgcttaa cttagtcgct aaattagtaa ccgctgaagt gattctaccc
    10741 ccaaaactac tactgatttt atctgcaaca gtactcgctt tagaactaac agagttgaaa
    10801 gcagctgaga cggcacttgt gatcttagac gaaaagctaa ctactgcact tgttgcactt
    10861 gtgaatgcgg attttatggg ttgagggatg gcgctcgata acttctgtac gctattttga
    10921 ataattgaaa aagctttatt gaacccattc tttatgggtt cgggaatttt ttcaccgata
    10981 gatgctgcta tcctttgaat ttgaccaaga gataacttga gacctgtact atatgcatta
    11041 ccgagtcttt gccctaatga ttgaccatta tttgctaatt gagccataat ttgaccaatc
    11101 atctgtatca ttttgttgct gttgttaact gcagcatcct gcgcttttct aaaagcattt
    11161 tgtgtagtac taacaattct agccatagct gcctcataat ctttagtatt tgcaccaata
    11221 tcagcgaata tagccccatc aaatgtacct gccattaaat ccctcctttc ttgttatatc
    11281 ttgttataaa ttttgaaaat gtaaattgag cttcgctaca cgttctgcga aatcgtttga
    11341 aatattagtt agatcttgtt tattttgata agttcgttta attttgttac gttgtttatc
    11401 taatttgagc ttattagcat tgacgttttt agcatttaac gtgtatctga gatttaaagc
    11461 aagttcagag aggttttcac gttctgcaat ttgcttgtac tgcaaacctt ccataatcgc
    11521 atctaattcc gttcttgtac acttgtaaat aatatcaatg tccgtcaggc caagttgagc
    11581 acaattaatt aagagattgc gtctttcatt ttcccaatca tgtcttttac gatttggatt
    11641 tgagcttcgt ccgcatcttc tcgagcttct aaatatttca ctgatttctc catgctttcg
    11701 atatatttcg aaatcttctc tttgaaaaaa ccagattcaa ccatgtcatt ctcaatttct
    11761 ttaaataagg cttctgtcga cccttcttct tcgactagct ctgcgataga atcaagcact
    11821 tcatcttctg acaatgcttt tttaccgctg cctacagaca gcttgactaa atctactaca
    11881 gctgaatcat cacgctctaa gattttataa aatagcgtac cgacgccgtc agcgttacgt
    11941 tcccctgtct ctttattcac agtcccaagt ctattattta tcttaaacat tgttttaaaa
    12001 tcaaatttaa tttcaatact gcgttttgct actgttaatt ccatttatgt atttctcctt
    12061 aaaaataaaa ggtagcgaaa tgctacctta taaattagtg tccttctgat aatttttcag
    12121 gaactgctcc cggtgaactt cctgaaactt cttttttctg gatattgtca taatcgccag
    12181 tagtttcgcc tgggttttga tatgcataaa cacttgttag caaagcgata tcttctgccg
    12241 tcagtggaaa tttgccatct tttaaactat cgataatatt caatgcatat gatgcttcta
    12301 caagatcttc aacaccttct gaatattcaa tctcgtcaat ttttccatat ccaaacttag
    12361 caggataaac ttctctatct tttaaagttc ctgaaccttt ttctttcaaa gattcgtctg
    12421 cgataacacg ccaaactttg acagatttac cagttgtttt agctatttct acaatttcaa
    12481 ttgctttgtc tttcggtgtg tagtatgatg tcaaatcaat tgagtgctca tctgttgctt
    12541 tttcaagcac acgtccttgt tgagtttgtt catcaataaa atctccaccg agcgtcgttg
    12601 tcccatctgt gcgatacgct ggcagaattg ctttactacc taattcagca tcaacagatt
    12661 gaataaaata aaatatattt ttacctttaa taggcttagc tgtagtaatt tcaactgtag
    12721 tttccattta attctccttt aaattagtat ttctgtgata ttgatagcaa tatggtagac
    12781 ctcacgacct acagaattat ccatcaataa ctgtgcatta atccgtttgt ttcgtcctaa
    12841 ggacataatc attctagact taacctcctc tgcatctgtt ctgctagtgg caggtaagaa
    12901 tgcgtcaata tttagcccaa aatctcccac aacagctcct gtctgcgctg tttttgatgt
    12961 atccgataca ttggaaccta tgacaataaa cggctctaaa acgtccgatt ttggtaattt
    13021 aaaatagatt gggatattaa gtgttcccaa tctatttttt acctcattca gatataaagt
    13081 tgatggtgag tatatcattg atcacctctt aaacattttt ttgaggttag ccattaacac
    13141 tggccattct tttctcaggg cagggtctaa aaatggttga gcttccattt tacgagtacc
    13201 taactcaaga taaatagaat ataaaacagg agaaaccact ctatagtgca agagtttttg
    13261 ttgttcattg tagatttgac ttctcagcca tgtagtatca accggggcta gtattttagc
    13321 ctgtctctca atcctagctg ctgatttgct aagtttttta tctacagcga ttcgtgctga
    13381 cttctgcttg cgctcaacac tgcgtaaaaa cctgtccagt cctcgcatac gataagtaag
    13441 actcatacat aaataaccgt gctattttta tggtgcgcta gtccctttat caacctcggt
    13501 ttccccttgt agataatctt gctaaagcca tcgtaatggc cttgcaagtg caacttaaaa
    13561 gcgttcaaat catatttacc aaataacccc atctgttcag attttgtgaa agtattttcc
    13621 atgcagggga tttttccgct aggtttttca acagttcttt tatctagaaa atcgtcctgc
    13681 tcatcgacat acactaagat aactctatct ttgtaaatca tttatgacct cctaaataaa
    13741 tctagcgaca ccttttccac ggcacctttt accataagca acatttctaa gaattgagtc
    13801 atattctgtg agatagtctt cccaactaaa agaccgacct tcttcagaat ctgccttggc
    13861 tccttctgaa ttaagccgat tatatctttt aatagctaca tctcttgcga taaatgttaa
    13921 acgtcgtggt atttctaaca gctcttcatc agaatactca ttgagtttcg caaggatacg
    13981 ttcaatactt tcgtcaatgg caagtttaat caattcgtct tgtgaggcat cattcttagc
    14041 aataccttta aaaattttta cctcaactaa aagtagttcc ttatccattt attttaccct
    14101 cctggaactg ctcctgggac ctctttatct gcttcaatag ttgcctctac aactccttca
    14161 ggaatttcag caaatagtac attcgctcca aaaaatactg actcataagt taagttagat
    14221 aattgacgtt gacgagctgc agcaatcaag ccagtctcat ctgtgtagtc tgcaaataga
    14281 ccacctaaat ctccaccacg tacattcaac gaagcaaaaa caagattatc aactgcagtt
    14341 gagtaaactt ttccttctgg cacagatggc ataacgataa cattctgcat ccctaaaaag
    14401 ttctttagta acgtcatccc aaaaacatta gaagcatctg cacctacttt agtatcacca
    14461 aggtaatttg ctacatctaa aggagataca aaagaaacaa gttgagaccc ctcaaactca
    14521 ttaaaagtag ctagttttgc ccatgataaa gatagtgcct tctgaagacc agccccttta
    14581 acttttgttg gttttgtttt gaggaatgta aagaacgcat ccttaattcc attctgaagc
    14641 tcacgcataa tgcgcttatc tgcttcagtg atagcacgag ctgcaccatg gcgagcaatt
    14701 gcctcagctg tagttgcacg acgtttcttg aaccacttca ctgtatagtc tttatcttta
    14761 gttcgagtaa ccttagacag agggattgtt tccccttctc caggatcagt ttgatctaaa
    14821 gtcacttccc acttataggt ctgaattttt aaatcactgg ttaactcttc acgacgagta
    14881 acccctagta gtttcagtaa gtcattaatg tttttggaaa acttgttaac aaaatcaatc
    14941 gatttaatgt ctcccaagtc tgccatggtg tttaaatttt tttcagccat aatattcctt
    15001 tcttattctt aaaataagtt acgattttca gcaattaaac gcatacgttc gtcgtcgtct
    15061 tctactgcca taatctcagc tttagtcatg ccgacagagc cgttgccagt ccgtggtggc
    15121 ttctgagtca aacgattatt aacgcgcttt tcaacagcct tatcaaaaat aatttgtaaa
    15181 ttatcaatat tagtcttaac ttcttctgca gtagtaccta aaacaacatc aataaattct
    15241 aatggcaaac ctttttccac aaggagcgtt tgagtctcaa tacgcatttc acgttctgcg
    15301 actgcttttt ctcgttctgc gattgcatca agacgttttt gttcttcttc cttagcacgt
    15361 tcatccttgg acattttggc caatcgttcg ccctcagtac gtgccttttc aatagcgtca
    15421 gattgttctg cttcccattt agctcgctca gcagtcatca tttttgaaat atccgcacga
    15481 gtaaatgtac gctcatgttc ttgcttagct tgttcaacag cttctttctg agtgtcgact
    15541 tgctcagtag tttctgttgc tacattttct tctgccataa ttttctcctt tagtggttac
    15601 gccaccaacc gatagtctag ttttacgtcc tgcgacgaaa cagtgtagct tttagtgtca
    15661 tcaacaagtt tggacataat aaaaagccgt atcgctacga ctaatctaaa tgattagtaa
    15721 ttatttcttc cattttttct tatagttatt ctttatataa tcaagttcgt tatttgtcgc
    15781 ttgagcatta tgttctacaa tttgttcaag ttgttcaatt cttttatgtt gatttctaag
    15841 agcttctgct tgcatggcat tctctgcgat aagcatcaca atagctcgtt cgagtttacg
    15901 tttcttctta attcgtttat tcattgcttt atccttcctt cttcacttct gcaataattg
    15961 acttaactgc tattgctgca atgccaataa tcaatatgag caccaataca cccagtgcta
    16021 ctaataccat tcgccaaata aacatatctt cctccttgat aattctgcta tactggtgta
    16081 tacttatggt agtgaaagac ggtggtctaa cgcccatgaa gcagttttct gtggaggcgg
    16141 tgggtcatcg tctttctttt tctactacag ctaacacctc gtctttgttt ttgataattg
    16201 atatattgag acccctgcga tctatcttat aaatatgttc taactgcccc agtatttctt
    16261 caaaagatag tggggttttt gtgatatcaa aaatgatatt aggtgcttgc ttttttgctt
    16321 ttcgtagatt accatcgatg acgtttttac ctgaacctgt gatttctttc aagtcaaatg
    16381 gaacgtcgtc aactaaatag tctggagtgt taatattttc ggggaaatta accctcggaa
    16441 ccatttcaac ttgtaatcct gtcttcttag ataaccagtt agcaactcta tattcgtagt
    16501 ttgaatggtc aagtactacg ttatgaccat caactctgta agttccgcct cctttagtgt
    16561 actcattcag catagatatt tgagactcat actcttgccc atctatccaa tctgatgtga
    16621 catccttaaa atacttagta cttttatcag cgataggttc aacatcggta ttaagttcat
    16681 cctcgtcagg aataattgta gtcctacaat taaagtgata tggagcagca ttaattccgg
    16741 gctgtatttc agataagagt ttctttttat tttctcttgc cttacttcga catatctgag
    16801 tagttctatc atctagctga actaagtctc gataatactt aatacctgca tcaatgtatc
    16861 gcttggctgt agcattattg ataatcatcg tgccatcagt acgaataagc gtttcagccc
    16921 tatgtctggc tacgtcgtaa cgtttgccca attcacgagc catttctcta ggaccaagac
    16981 ctctgacaaa tccttttttg aatgcttctt gtaaatcttt tactagatta tcaacatttc
    17041 cccagagttg ctctgagtaa ttatagccgt caaatggtgt cttgattaac tgttctagag
    17101 ctggcccatt aattgttcct gagcgaccac ctacagattt ttggtaagca taactagctg
    17161 cctttcttag atgatcgcta aacaaaccat ctaagatacc tctcatagta cctgcacgat
    17221 gtataagctc taaattaagc atgtcaagac gagtagcctg cgctgataca tattgttcat
    17281 taatacgttt aagtagctct gggtctttct cagcttgctc tctatatcga ttggcatttg
    17341 ctgcataatc acttaaatct tcgtgtctaa gcttcttaac agcatcttga taagttatct
    17401 tattatcttc agcaaaacga ttataaaaat catataaatc cttttgtaaa gacaatgatt
    17461 ggttactata tactctagat aattcattga aaatatctaa gtctgctcta tctaaataac
    17521 taacaatatc ttcttgacgt ttttgccagt aatcaagatg gtcctgctgt tggttcttct
    17581 tgtccattag catcacctac atttcttggt tctggcatta cttgcttctc gtcagcctct
    17641 tctttcagac gttttaattc agcttcagca tcgacaccag ttactgcgtt taaaatttca
    17701 aaaatagttt gatcgctaac aattccataa agactctgtg cagccgaaac aatttcatta
    17761 tcattctgcg gcacattagg agtaaatact atactagtat cattaacttg actatatacc
    17821 gtcgattcgt tccccttgat tgaccagata ttggcagcta aacgtaaacg tctcatgagt
    17881 ccttttttga acaaccgttc ttgcttttca cggtaattat ctgaagccat tagcttatac
    17941 ttcattgact cgccagattg aacaccagag aattttaaat cttgaatatc gggagtaaaa
    18001 gtaaagcgta agatatctga cactagtctg tttttatacg cttcacttcc tgccatatca
    18061 tattcttttt ttagataata cgcatctggc ttagcaccat ccgggttagg attgtcatca
    18121 aggacaataa cttttgcctt cttgaatcca atagatatac ctaaacgacc gtttggattt
    18181 atacgtccat cttcaaaaaa atcattttcg tctgctcctg tatacggatt accagagaga
    18241 actaggattg catctacaga atcttgttga aagttagcaa gctctgactg tgataagtcg
    18301 tatgcatcaa tattatctaa gactgattca taagcgcctg ctctatcatc attattagaa
    18361 tattcattaa ctggaacacc actaaagtaa tgttcttctt catctttcag actcattcct
    18421 tttgtttctt gattaaagtc ctcgtaagta aatatcatat ttgaagtata gaccttaaca
    18481 atttgcttac gtttacctgc gccgtaatcg atgtcgtaga aatgtacagc catcaaagaa
    18541 ttatgttgat aagtatcatc ataaataaca aatgtctgtt ctgcgggtag ttgatagagt
    18601 ttaatttctg tcccattatc tacttcttca acggttaaaa gctcgtaagc acgaccgtaa
    18661 atggataagt ccgttttgat tgctacattg tgatagtctt cgttgttacg aacactcata
    18721 agattaatgt ttttttgaat atcactatct tcgttcttgt actcgacagg cacaccaagc
    18781 atataacctt gctcaaagac tgtaatgtac ttagcaaaat cactagcaat acgattgtca
    18841 gctgcatatt catcggtctt tgctggacga tatttgatat tgttatcacc aagataatat
    18901 cgctttaact ctttcaatcg gtctagttgc tcagtctcaa aactagaaat atattttttt
    18961 aactgatcaa tccatttctg cgaattatat tcaatagcat caaaatcttc ttgcaacatg
    19021 ataaattgcc tgttcgcatt ttcattaaat cttgttcctg ataaaaattt tctttcaaac
    19081 aagttcaaat ccctctctat ctaaaataat atttagcatt atttagtcgt ttatttctgt
    19141 cagatgattg atatattcta tcttgtactg catatctgat agcatcaatg caatggttat
    19201 agctgtcaac tggctcattg atatactcgt tagttttctt atcttttttc caagtgtaat
    19261 tctccagctc ttcaattgtt ttaacacacc tttcgtcaac aacgatatcg tactgtagca
    19321 tgtactgaat tccttgcata actgaaccag ctcctttttt tacatcaatc attctttgaa
    19381 tacctaaatt tctcaactct tggttagatt tcttttcagc tgaatcccct ctgatttctt
    19441 ctttggcata tccgaggctc tttatagcat tagctatttt atcattggtc aaattctttc
    19501 tgacatattc ctccaagatg tatagcttct tgtttgcatc atcgattttg acatgcaaaa
    19561 atgccgaagg atcattaatg aatccgtagt ccaaaccaaa aaaagaaggc aagtgtgata
    19621 acttgtcttt gtttagtatt cttttttcat actttggaaa tatgagcttt tctaaagtcg
    19681 cgaactgacc tagagcataa atcttgtaat aagcttcgtt tctgtttgct agttcctcta
    19741 tattttctct agtaacatca tctaaaaacc tattatcttt atatgtagtt tgataaacta
    19801 cagtgttttt aggtgacttg ataaagaaag ctttataaac ccagtttgct ttactcacag
    19861 gattgaacat gagatagact tgcttctcta gatgtttctt atcccttaaa cgcaaagtca
    19921 actgtgtgta atcatcaagt gtaaactcac tagcttcttc cattacaaca tcagatatcc
    19981 ctttaattga cttaatcttt tctggattat ccatgccttt gaaaataaat tccgagccat
    20041 tcggcaatgt tattctaaaa gctgacatat ttatcttgca tttatccaag acgccaaaat
    20101 acgacaagtt agacataata tcagcaaata cagagtctct gacagttgca ccaacttttc
    20161 gaaggacaag aatctttctc ggatgtttaa actttggatt gagtgctttt aagataattt
    20221 tttggaatac accgtgtgat tttccagaag atgcacctcc atagtgtacc tctgtgaagt
    20281 tatcgtaatt atatagttta tcgtagatat gtttattaaa gactttgcta ggatacttga
    20341 tgacaatatt aattttaggg cgttgcttag tcgtcatccc actcacctac actgatggtt
    20401 acatctgaac ttatatccat ttcaattttg tcagtgaata atctgtaccg cttacctagt
    20461 agctcagctg ccttgattct atcttttgca cctacatcaa tatcgacaat ctcttgtcct
    20521 aactccccga tgctgatcag tgttttttct tgctgatcgc ctcgcatgat tgatgtaagg
    20581 tattgtaaaa cttcttcttg cgtggcaatc tttgcagatt caagcttttc aatcctttca
    20641 tctatataag ctttaatgtc aggtttggtc aagttttctt gacctattga cctcgctgtc
    20701 tttttactgt aacctgcttt aatagccgct gctgtcgcgt tagctgagat gatgtactca
    20761 tctgcaaaac gcttttgttt tagtgttaat ttactcaatt ttccatcacc tccttcgtga
    20821 cataataaaa agacaagaca ctatttgcct tgccttcaaa ctcatactac caatttatca
    20881 tcaaaaaagt gagaaaacca cccttttttg tctcaaactt acaattttcc aatttgttgt
    20941 gcaaatagag tcaaaattct atcccttttt cggtaggttc ctgctctaga cgtatgaatc
    21001 ttataagcta tctcctccca ggtattatta gaaccagttc cccaccttag atcaaaaata
    21061 taattcaatt catcgtcaag cagtgcttta accctatcaa cactttctct gaagttttca
    21121 taacttttga ttttaccgtc ctttgaccac tttatgacaa tatcttctgt ctctttagct
    21181 actagattag acttcccccc gcctacattg atatctgtac taacaggcgt ctgcagctca
    21241 agttttctaa ttgcaatttt acgttcaata tcgcgataat caaacaacca ctcatcaaaa
    21301 gcctttaact gtgcgttaga taatttactc attccgtcac ctcttctcta aactgccgat
    21361 aataaacggt aaaaataata ttaaaagtat caaaacaact gctgtgttat catccattct
    21421 tatctcctgt tctccaaaaa ataaataaat ctctacttag tacaactggt ttaccaaaga
    21481 ttttatatga tgactcaaga ttttttattt caacatcaaa accatcacca agtcggtatt
    21541 ttagtaaatc tattgttttt tggctgtcaa gtcgtctagc taagtattca tcattttttg
    21601 gaatagagat tttataacca gagaatcctt tcatcgcaga ctgtttaagt ttctcctcga
    21661 tttttaaacc atcaaagtac ctatcaaacc attttttgtg agattctgaa ccgcattctt
    21721 ttacttcatc aattaatgac aactttatcc cccatttcct gtcaactccg caatccgctt
    21781 tgtctgtctc tgattttgct ctgacgactc tttcaaacgg tactgcgtgc gtattagttg
    21841 ctgttgtaaa cctgtgattt gtggttggta gtagttgtac aatgcaatat ttgttgttaa
    21901 caacacgctg actgctacag ttaatacaac aatcacaatt gactgtttgt gtaatttgtt
    21961 gttttgttcg ttcattcgtt cacactttct agtaaccaat attcaacgag acgttttgta
    22021 tattcgagtg ctttttctaa atcctgagtc gcattgcctt tgtatctatg tcgtttaatg
    22081 tatttatcaa tgtgactttc catgattgcg acaaactggt cccatgggta tgtcttatac
    22141 cacgactcaa tcaaatcaat ttccccatga ttataatgct ttggtttatg tactaattca
    22201 cctgtcattt tgttttcctt tctagccact cccaaatcat gtggaattgg ccattcacca
    22261 aatcgtcgtt gccataactc tcgcaaatat caacgattga catattaacc catacccaat
    22321 attgttcagt tccaaaacca agctcctgca tttttttgtt actttctctc atccagccag
    22381 gaaccactcg ttcaaaaaaa tcaatataat caattttcat aactcttcca ccttcacata
    22441 tattcctact gtatcagccc agaacttctc aataatttcc gaagcaacct gcgcatcatc
    22501 attccaaaat cccaattcag tcatgcaatc tttaggtaac ttgttcaaat tatctgtgtc
    22561 gggtttagta tctttatatt gtccgtttac tgattttttg attttcggaa ataaccactt
    22621 ggttgttagt cttattggac catttaattt ttctttaggg acatgttgag caaacttatc
    22681 cataaacatt gccctagctt cctttagtga atctggttca tagaattgtg gtttcccatt
    22741 taccacacgg accttttttt gctgatgtgt tgtggttggt atttttttca ttgggataaa
    22801 aaattcaatt gtcatgatca acattatcca agtttatttt ttcaaattgc tgcacgttgt
    22861 tctaaaattt ttagataagc tcccattgca tgccattgtg ctactaataa ttcaatagga
    22921 cattgtaatt taaaatctag actatcaata taaaatttat taattaatac tgataatttt
    22981 tctgcacggc tttttaattc ttgatattta acaatcattc tttttttgta ttctttcatt
    23041 tttgtcactt cccatctttt tttattttcg cgcatagtca atgtcaaggg acatggttac
    23101 agggttacaa ggggcggatg catagccccc ttgttcctgt tcatgtaccc attgaccaat
    23161 tgggacattt cctaaatatt cttctttcgg agaaagagaa tattctgtcc ctcaattttg
    23221 tccctcaaaa gtcaaggaca ttttcgagaa attttcgagt ttgtccctat ttttgtccct
    23281 gaaaatcatg taatagggac acggacattt tcgagtttgt ccctattttt cagggacatt
    23341 ttcgagggac actttcgaat ttgaccctcg attttgtctg tccctgtccc tgtccctctg
    23401 tgaaatattc acaaactttt attggtttta ggttcttcta ttggtaaaat ttgtttattt
    23461 ttaacttcaa atttaccgtt ttcttttatc catctacgaa tagttttttc agaaactggc
    23521 ttatcttcag tagaaaaata ttcgattaag ttatcgattg tgactggctc tattccatca
    23581 ttcaatgaat taattgcagt ctctactttt tgagatttct tttccctgga ctctcttgga
    23641 ctcttcttct caaaattctt cttccaagtc ggcacattat cttctagtga tatatcagcc
    23701 aagacacccg ttgtgtctac atcatgcact ggatagctaa accacatgtt aactggttgg
    23761 aatttagcaa actcgcgtaa agtcccttca actcgccagg cagtggaaat ctccacctca
    23821 tactgggctt tcttgaccgc ttccagatag ggctgtttaa tcatgatgtc cttgatagcc
    23881 ttgtcaaaat gttgctgcat ctgataacga ctttccaaat catctagcgt tacttcctgt
    23941 tgatagtagg ctagcgcttt ttcttgcaag gcttgttggt aaatcttagc ggctgctttt
    24001 tctgagcgtg acttgatgag ttcctcagtc aattctagct ctactaggtc aatcagtgca
    24061 tcagggtcac gggcaaacac tcctgaacca cttgcacggt ccatagattt tttaccgcct
    24121 tggctcccct ttgagtgatg gtggcagtaa atcacactac aacctaactc agtagctact
    24181 ttgtcaaact gattggtaaa gtgagccatt tggtccgcac tattttcatc accagtcagc
    24241 accttataga tagggtcaat gatgaccgct tggtaatttt tctttaagct acgacggata
    24301 agcttaggcg ctaacttgtc catgggaacg gtctttccac gtaggttcca aatatcaata
    24361 ttcttgatgt ttgctggtgg caagctcata gcttcgtata catcacggaa gcgatgcaaa
    24421 gctgacggtc tatccagttc cagattgaca tacaagacct taccttgttc acactgccag
    24481 cctaaccatt ctttaccttc agccagcgcg atggatagct caattaatgc aaacgactta
    24541 ccagcttttg atggtccagc aatcaacatc ttgtggcctt gacgcaaaac tcctttgatg
    24601 agttccggtg ccaattctgg catattatcc cattcgtcag ctagcgtctc tgggtcaggc
    24661 aaatcgtcat ttaaatcttc aatccattga taccattctt cgtagttggt tttacccaca
    24721 ttagtatcaa tcaagaattg cttgtgccca ttccgagtca caccaggcat gcgtgataat
    24781 cgactagggt tgcggttctg tgtgtcaata tcaagtccat tctttttaca aatttgatag
    24841 atataatcaa cccgtttgcg gtactcctga taatcacgag catctacttt gacaatagcg
    24901 tgtaatgatt ttttaccgct atggactaag gttgctattg gcaattctag ttctttaaac
    24961 aaagcatact gtttaccaag ttccatgctg tcggattcaa ccaaggcata tctaaagtct
    25021 gttacattgt cattcttgac accatttcca tctagtggat taaaacgaat ccatgcacca
    25081 gcctcttctt tgaagtctcc aaatactgct cctaagtcag taggattttt ttggagtgat
    25141 tcaatcagtt gaccagcagt cctgtcatac gctccttttg ttggtttata aacaatctct
    25201 cccgtatcaa gggtaattgg ataggtctct gttacataac caactttgtc agttgattca
    25261 aataaggtat ctatgtaagt aattaaatct tgaactggat tccagtttct tggctcttga
    25321 atctctttcg attcaatcca atttttatcg acaatcttat aatcacgatc gattgtatcg
    25381 tcccagctga gttcatgagc atcatcgctg ttacgatact ctgatgatgt ccatccatta
    25441 tctttagcta gctgtgtgat tgttgcccct gtgatagtcc caaagccatt gccttggaaa
    25501 ctatcccatt ttgcaaaaca ctcacctttt ttatatctac tatcagattg tgaccaaaca
    25561 tcccaatcca ttgctgtata accttcatgc tttagggcca ttcctacttg aacccatgtt
    25621 tgatagtcta ccgtggcagg attgatataa tccagcaacg gtagcaaatt aaaatcattc
    25681 tctgccatgt tatcctttct gcacatgtta tcctttctgc atttgatata attaacctaa
    25741 agaaaggagg tcaactatat gcctgaaata aaaattactt tttcagatgg ttctattgtt
    25801 atatttcacg aagaacagag ttttcaaagt gtagtaaaat caaatgatgc tatgtcctta
    25861 tcaaaaatat attcattatg gaaccatgtt catgatggat taattcctag cttcttagaa
    25921 ttaattgcta actcgcaatt tttctttgac ttagaaaacc cacaaattta ttacgcttca
    25981 aatgctgtta taaaaattga agctatttaa taattctgct attcaaggca atatctctga
    26041 ttttttcttg aatagctttt tttactactt gcccaaactc ggtgagactt gagtgtttat
    26101 cgacatataa aattgcctcc gaatatgttt cggatttttc gattgcttca tcagcaaaat
    26161 ttgaaataaa cttttcaatc tgttcattaa tattttccat tttttctcct attctggttg
    26221 atattctcga gcattgatgc cttttggtag gcgccatccg ctagcagcaa tacgattaat
    26281 catgtttgat gctacttcaa atttccacat acctacattt ttgaaaccgt atcgctcaag
    26341 cattcgtatt tgttttggag ttgttagacc tgattgacga cgtttatcca gtctatctaa
    26401 taatttggca gccttaccag cattcccaat ttcgtcagta aatatgccaa atttctcaag
    26461 tgttttaagt tgtttttcag aagctggcgc catctcccaa ccaaattccg gtacatagtc
    26521 cactaagtct tcagcgttga tagacatttc aaattgcaac ggatcaacta atgcacgttt
    26581 acgtttacgc atttctgcta actgcttagc gagtgcttct tctcgctctg caacaacatc
    26641 tttgctggct acttcttcag cttctaacaa ttcaaaagct acttcagttt catcggtcat
    26701 gttctcgacc attttttgtg caacttctgg tgtttttgca attagatgag caggacgaca
    26761 aagctcatgg cgttcggtat gccataaaaa atctaataac agtagttttt ctttccccgg
    26821 cgctaatcgt gtgccacgac cgaccatttg actataaaga gctcttactt ttgtgggtct
    26881 cagtacaact acgcaatcga ctgtcgggca atcccatcct tcagttaaaa gcattgaatt
    26941 gcatagtaca ttatatttat cattatcaaa gtcttctagg acttcagcac ggtctttcga
    27001 ttctccattg acctctgctg ctttgaatcc tttttggttt agaatatcac ggaatttttg
    27061 ggaagtttta acaagtggta aaaatacaac tgttttgcga ttagaacatt gcttaaccat
    27121 ttcatcggca atttgttcta ggtacgggtc aagcgccgtc ccaatctcac ttgctttaaa
    27181 atctccagct tgctgactca ctgtagatag gtccaaagtc aaaggaatag taactgctgt
    27241 aatttttgat aaataaccag atttgatagc atctaccaaa gaatactcat aagctaaact
    27301 atcgaaatat ttacctagat tttgtttgtc accacgatct ggtgttgctg taactcccaa
    27361 aacattgcta ctatcaaagt gttgtagaac tctttggtaa ccgtctgaaa tagcatgatg
    27421 cgcttcatca acaacaattg tgtcgaagta atctgttgga aatttactca aacgcttttc
    27481 tcgctgcaac gtctgcacag aaccaacaac cactcgatac catgagccga gcgatgtact
    27541 ttctgctttt tctaaagccg tgcccaatcc tgttgctgtc attagcttat cacttgcttg
    27601 ttccagcaac tctgaccgat gagccaatac taaaacacgt tcgcctagtt taactctatc
    27661 ttcaataatc tttgagaaga caatagtctt accacaacca gtaggaagga caagaagggt
    27721 tttcttcttg ccttgttccc actcttgttg cactgctgac cttgcctctt cttgatattc
    27781 tctaagtttg atgtaaatca cactcctttt tgtgtaggtg tcgttaaagc ttttgtaata
    27841 ctccaaccac gatttaatct tgtaagtaaa gtaatattag aaataccaag ttcacttgac
    27901 cattgtttta ttgtctgagt cttaccttta aatgtgatta aatggttatt tctttgatta
    27961 tttgcttgtt cggttgaatt agcccatcta caattagatg gttcataatt tccgttaaca
    28021 tcaatacggt caatagataa attgtctcga taaccattcg atgatgccca ttgtttgaat
    28081 tgaatatatt cttcccattc gtcacatata gttattccac ggccaccgta tttaatataa
    28141 tcattggaat ttttatctct gcagcgttgt ctcattccaa cccaaatatt ataaagacgc
    28201 tcttttctag caaaaccatg tttagtaaca atgcatccgc aactggtact atgaccagtt
    28261 gctagattac tactattaac aatcgttaac ttaccgcaat cacattgaca tttatatttt
    28321 gcttttgtat atggatcctg aggtaaacgc tcaatcacta caagcctacc aaatcgttga
    28381 ccagtgtaat ctttagcttt tcccattaga atcctcccca tccaccttgt tgttgagatg
    28441 cttgcatttg accttgttga tatgactgag gtgctggttg ttgttgaggt gcaaaattag
    28501 ttgtttgctg agctggttgt tgatacgttg gttgtggcgc ttgtggttga gtatttgcat
    28561 tcaatacttt agtccagtca acgctatcag cataaatcat cgatttcact tcattataaa
    28621 cattgtcgtt atatgttcta ttcttaacac tacatacacc cgtcgcacca actactgtgt
    28681 tccaattcat ttttaatggc tcaccgtgtt ttttctgacc aattgcccca aagaatgctg
    28741 ataacattcc ttcggtagag ctatgtaaaa atagattatg cttcaaacta gcaacacctt
    28801 catcagtttc aatttcaaga gaaattactg ctttattaca tgctggcaat ttccctgttt
    28861 tttgtggatt tggtgtgtgg cgtgcacgtt caaagtctgt tactgtgaag acatagtcac
    28921 caggttgtaa tgtaataaat tcttttgcat cttcttggat tgtgtcatcc catccaaatt
    28981 cacgttcaaa gttgttattg tattcagtca ttttatttct cctattttaa ttttaaaaag
    29041 tattgttagc tattggaata aatccaattt ttgttatatt ttgctattat ttctaattcc
    29101 caaaatctat gagccttcca ctgtaaaggg ctgttctgtt gctcggactt gtgttttaat
    29161 aacttccaat gtggctcccc aatttgctac aatcatgttc cagtaatctg gtggaaagtt
    29221 ttcaatagga gtcaatggag ggaagtgtcc tctaatattt gctactgcta ctaactcatc
    29281 tggtgtaact tgttcaggcg tcatcaaatc cgtcaaagcc tttggtagga cagatggata
    29341 ctctgttgct gcagttggtc ccgacatttg cgcctgttgc tcatgtacag gttcgtgtat
    29401 aggttgagta ggcaccggtt gctgtactgg cggtgtttct gctactggtt gacttggtgc
    29461 ttgtgcgaat atatgtgcga taccagcgta atcaagtggc atttcttctt gtaatccatg
    29521 acggtttttt gcatcccacg ctggatgatg ttgtgtatat aagacacggg taccgccagt
    29581 tgctttcttc ttgttgttat cagctgtcat cactacagtt ttataattag cgaacagaac
    29641 catgtcagcc cattctttaa ctaatggtgc agtctgtgag cttgtctttt tacctagctt
    29701 gagttcccag cgatcataag ctcccatttc atcaggttgc tcaaatttac gcatttgggc
    29761 atgagcagta aggacgacat tgatacctaa ctctatcaat tcctgcaaca tatttaggaa
    29821 acgtccaact tcttcttttg tgtaagtgta accattaccc caaccaaaat cttcaattcc
    29881 tttttttcca tgttgagcac agacatcatc tactattaag ctttctgccc agtcaatagt
    29941 gtcaatgaca agtgtcttac agcatgtagg gttagctttt acaaaagcaa tttgattttt
    30001 tagcatagtg tagcttgtag gcttgtccat gcgtgccacg tccatgttgt cagttgaacc
    30061 ctctgtgtct ataaacaacg gctctggaaa ttgcgctgca aaacttgact tcccaattcc
    30121 ttctggtcca tagataatga cacgttgtgc ccttgcccgt tttcctctag taatttgcat
    30181 atttacctct ctttctaaaa tccaccttgc caagtcggtg ctactgtttc agcaaaacta
    30241 ccaccctctg ctgttttaaa gctatgggct ttattttcca ctgagtatcc atcttctata
    30301 atgatggcac actcttcgcc agttgataca cgggttgcaa tagcttgcaa accctcctgc
    30361 tctagccatt taccaaattg ttcaagtgtg atgttgtcca tctgttcgag tttatcaatc
    30421 aaaacaaagc cacaatctgg ttttagttta cgtacaatag cagttgctac tattagttgt
    30481 tggctgcctg acatgttatc ccactcttga ccaaggtaaa gtaactttcc atcatctact
    30541 gacaagcctt caagtggcag atctgcatta atcaataagt ctcgtttttg ttgacgaatt
    30601 gactcaatat cattagtcaa tatgttatat tgttcacgct gatgtttagc atcttcttca
    30661 gctttatctt tatccagatt agctctgact ttgcggttga cttcttcaat attagaaatg
    30721 ttttcttcta tttctgcagt tgattcatct attaatgaca ttgtgtctct ttgtgcaatt
    30781 tccaagtcac tagataactc ttgtaattgt ttctctgctg tggctaattg ttgttttaaa
    30841 tgttcaacct ctgactgttt aaattgatga ttttgttgaa taacggatag attctgacgt
    30901 tttctagcgt tttccccatt ttttgctaac acttcttgtt gttgctggat taaatcagcg
    30961 atactaatta attcttttgg ggcatcatta tagtatggtt gctccttagc aaacttttct
    31021 ttttggtcag caataacacc aatagtatga cgttgattat agagttcttt ttctttgagt
    31081 tctaattctg ccaactgatc accaactcca atgatttcaa gtaagacatc tgctttttct
    31141 tttggagttc catccataaa ttttggtaag ttaatagcta actcttctac aaagctatca
    31201 agcaattgtt gaccaccttt ttgcccgtta ggatcaataa ctttcaaact agaattctta
    31261 cccttacgct caacaatcaa accatttgac atggtaattt taagcgtagg tggtaccata
    31321 gttccctctc gtgctgcttg actaggttta aacttattac cacctaatac ccaagcaata
    31381 ctatccagaa cgctagtttt accctgattg ttattgccac cgatgatggt taatcctttg
    31441 gatgatggtt caacttttac agctttaatg cgctttacgt tttcgatttc taacttatta
    31501 attgtgattg tcattgtcaa aatctcctat tactaattta ctttcggtaa ctactttttc
    31561 agaactaata atttgagcgt tagcaagacc atagtttagt agtgcatcag ttacttcttt
    31621 tagtgttaaa tcacattcat ttgcaatatt ggcgatttca tcataaatat tatttgaaca
    31681 tctaattcgt gagtacgtat tttttggttg tctttcaact tcaatcatta tcatcactcc
    31741 aatttttttc tagtgtttgt ctatcaacgt tatctaactt agcaatacat ttttccagtg
    31801 tgctatctaa gatgtaacct tcgttgacga taatatccaa taaatttgct tttgctacgt
    31861 tcgtaaaata ataatctgcc atttcattag ttagtcgtct attttcatca cgcaacagtt
    31921 cattttctag taatacttct tttaacatgt acgccctcta ttattacgtt caatcatatt
    31981 gtcgtaacgt cgtgcattag cttcccagcc atgtgtttcg attgtccatt tttgacgtgt
    32041 ttctataacc tgatttttct ttacaaataa ttttttaaat aatttcttca ttatgttata
    32101 ctcctaactg tctttccttt ttaatatttt ccaacatttc tgccagcgtt tcctttttag
    32161 gtttatatct atttctagac ttccaagtaa caaacaactt aaatccctcg taattgatga
    32221 atacaattct atgcgttgga ttattgataa attgtttaaa atctggatga tttctcattt
    32281 ctgctgccca aacctttgcc gttccgacgg ttaatccctc ccagatttgg caaagatgca
    32341 cataatcgcc atagtcggct ttttccgact gcttagctgg tctgtagact aattcagctt
    32401 ttggcatttc ttacctccaa tctgtgatat aatgtaagta gaatttttga taaggggctg
    32461 actgcaatca gttccttttt ttgcatttac tcaatgctgt aatctgcaat cactcgcaga
    32521 ataaactggt tagcctttgg tcctctagtt gagccactaa gaatgttagt gacctcttga
    32581 cgattgagac cataggctgc tgctaaatca ctcttcttga tattgttact ttttagaaag
    32641 gtaacaactt tttctcgtcc aactgcaata tctggcatat tttctccttt cttttctgat
    32701 ataataaaaa taaaaacttg aggtaaaaat gtctcgatac tctgattata aatacgctaa
    32761 tactatcctt aaaagtattg aagaacgacc agaaaaatat ctaattatcc attactcctg
    32821 cgaaagtttt tataatctag gtagtaaaag tcctagaata gcttccattt cagttcgtca
    32881 attcaataat gcccaaacca acaacttttc cattcatcag tattccgaga tattagatac
    32941 cccaattact gatgatactt atgaaactat cgaaaaagag ctgttaacag atttcttcac
    33001 atttgttgac agaaataccg acaaaaattg gattcactgg aacatgagag atactgtctt
    33061 tggctttcat gctattgagc agcgattcaa agttctaggc ggcacaccaa taatcattga
    33121 tgatgacaaa aagatcgatt tagcctatct gtttaaaaag atgtacggtg gaggatatat
    33181 tgacaatccg catattgaaa aattacttga attgaacaat ctaaccccac atcgttttct
    33241 gacaggtaaa caagaagctg acgctttctc ttcgagacaa taccatgaac taagtatgtc
    33301 aacatcaagc aaggtcaata gcttttcaac attcctcact ttagctatca acgatgattt
    33361 aaaaactaac acccctaaat ggaaaatgag aggaactaac ctatctggat tactttccgc
    33421 ttttcaagaa acaacctcag gaaaagtgat aatcggtata atcaacctta ttgtcggcgg
    33481 aatagttggt gcaataattg caaaatatat tcaataaatg atttcttttg aagtagaata
    33541 agtttttgtt gtacttgctc tacttctttt tttgcatcct ctttggaaat aagagtcgga
    33601 ttgtcgtacc aatctctttc cacgtaaagt tcgttttctt tcccctctaa gctatcagcg
    33661 acccactgaa aatgctcatt acgcttttgt agatcaacga tgtttttttt cattttccct
    33721 ccttgtttta aaatctgtaa acaagaaaca actaaatttt caacttttta gtatataacc
    33781 acttgacaac tcacacctaa aaagttagaa ttaaagcata ttaaagaacc ttaacaaatc
    33841 aactaaaacc cgttcgccaa aactgtttta tagttgtttg cttagttgtt tttttagtta
    33901 tctcttgctt acaaaaaata tgatacacct atttggttga attgtcaaca tttttcaccc
    33961 aaaaagttga aatatttttt gtcatgcctt agaaaggttg ataaatcaat gtttccgacg
    34021 tttgaaaaaa tcaaagaatt gtgcaaacag cgtggaatta cacttattca actagaggaa
    34081 accttaggat atagccgaaa tacactctat aagttgaaaa ctcaaaaacc caacgctgaa
    34141 agaattgctg aaatagcaga ctacttcaat gtctctacag attacttatt aggtagaaca
    34201 gataatccca atatagctaa agatagccaa gaatacactt ctgacgatct tagaaaaatg
    34261 gcagaaaagg ctaaaacatt tgatggcaag ccacttactg aatcagatat tgatgcaata
    34321 caaaatatta ttgaaattta tttgaaagga agatagtcta tgactattga agagattgta
    34381 gactgtcaca aagttaatct cgcatacttt gataacgaat tatggccacg acctgggatt
    34441 tatatagacg atattaaagt tatttttgtc aataaatcac tatcgttaga tgctcaaaaa
    34501 cgggttatcc tgcatgaact cggacattgt gaaagaaata ttggtgacta taagacacta
    34561 cgtgagaaac acgagctaga agctgacaga tacatgatta gtcatttaat cgctgaagca
    34621 ctttctcaat tagaagataa acgagagttt aactacctac aatttatgca atatcatcaa
    34681 ctaacaacta ctgcagatga atgcatggta attgatgaat actataattt acttaaggtc
    34741 gtgtaatata gtgaggagat tatatggata taaataaaat aaaggaattt gctgtgtcaa
    34801 catcagaaaa ggctaaagac ggcattatta aagcaaacga aatgagaaaa agggcatcac
    34861 aagaaagcaa aatagtctta ccgcccacaa ttaatttagg ttttaataca cctactgcta
    34921 ttcgtaaaac agtggagggt gactactata ttggttttta ttctgaaaaa cctgatttat
    34981 ttaaatttgt ggattttcag tttgatggtt cacgaattat tgaaaaaaca gtaacaaaag
    35041 gaaaaacgac tcaaaaaggt cgttctggta gtacaattat tggaggatta ataggtgcaa
    35101 ctatcaatcc tactgctgca gtacttggcg gattagctgg tgcaaatcgt ggtaaaaaag
    35161 gaactattaa cagtacctct actactataa ccgaggaaat tccgggtaaa gctcaattaa
    35221 cactcagaaa tttaaaaaca aatgatataa aaataattaa aacaaaacta actcaggctc
    35281 aagctaacaa tattcaaaat ttttttagca aatagaaaaa gccccactct agccggcaag
    35341 caatgaagtg aggcaaactg tacaataaat taggcattaa aaagcccttt ttattgtacc
    35401 cattttatca agaaatgagg taaaaatcaa tgtggtcaga aaaacacaaa agtggaaaag
    35461 taaattttgt agaaagatat aaagatccat atactaataa atggaaacgt acatcagtac
    35521 tgatggaaaa agatactcct cgaattagaa aagaagctca aagaatatta gatgctaaga
    35581 tagctgatat cgtaagaaag ttgcaaacct ctgacatgct atttacaaat ctaattgatg
    35641 agtggtggat attttaccaa caggaaatta aaagatcgtc tattgtcact ttaaaaggca
    35701 atattcgaga gattagagca gaatttggga tcaatatacc tgtagttaat atcgacccta
    35761 gatatgtcca aaattatctg gataatctcg attgctctag aaacaaaaaa gagcgaaaca
    35821 agtctatgct taatcttatc tttgattatg ctgttagtct agatatcatc aaggataatc
    35881 ctgcaagacg cgctaaactg ccaaaaatca agaaaacact taatgattgg aaaaagattg
    35941 aagaaaaata tcttgaagaa gaagaaatca agcggttgtt aaaagaatta tttagaagac
    36001 ctagcactca ccgcctggga tttctatcag aatttatgag tcttaacgga tgccgtattg
    36061 gtgaggctat tagcatagag ccggataata ttgattttaa aaacaaaact ctacagttgc
    36121 acggaactta tgatcgcact aatggttaca taaatggaga aaagacctct ccaaaaaccc
    36181 ttgcgtccta tcgtgaaacc attatgagca aacgtgaaat ggaaatttta caagagatgg
    36241 agtttattaa cgaactcgaa aaaaatacaa ataaacgata cagagacatg gggtatatat
    36301 tcacgactag gaacggtgtt cctatccaaa taaattcctt taatttagca ttgaaaaaag
    36361 caaacgagcg gcttgaacaa ccaatcaata aaaatatcac tagccacatc ttccgtcata
    36421 ctttagtcag tcgtcttgct gaaaataacg taccactaaa agcaatcatg gatcgtgttg
    36481 gacatgctga tgcaaaaact actgtacaaa tttataccca cattacaaag aaaatgaaat
    36541 ccaacatagc tgatattatg gaaaactact aactttatgc cccaaatatg ccccaaatat
    36601 aaaaaaagaa ccttgtcgct atcaataaac attgatatga caaggttttt ctagacgaat
    36661 tatttaacag cgtctttaag agctttacca gctttaaatg ctggaacttt tgatgctgca
    36721 atttcgattt ctgcaccagt ttgtgggtta cgacctttac gagctgcacg ttcacgaact
    36781 tcaaagttac caaaaccgat taattgtact ttttcacctt cagcaaggta atctgctaca
    36841 gctgcaaaaa ctgcatctac tgctgctgct gaatcttttt tagttaattc tgtagcttct
    36901 gctacttttg caattaaatc ttgtttgtta gccatgttaa caaatcctcc aaataaattc
    36961 taaacttata caacataagt tttaacagat tatatgatat ctaaaaaaag gcttaaggtc
    37021 aagtatttaa cgtcatttta gggctttttc ttaaaaatat cgaaactaaa attatcgttt
    37081 actaaatcaa ttgcagtcgc tttcaaataa ataccttctt tattttttaa atcttgcaaa
    37141 ttaatattaa taaccgattt tttaggtttt atatctacaa agtttggcaa tttataagat
    37201 gattttatat attgtaacac atctttttct ggaagcggaa gagttcctac tgaaaatgag
    37261 gtcactttaa gttgaacagc gccatttgtt aaacgataag gttcaaaata gatgtataaa
    37321 ggcacttcat aacctaataa ttgataagag ccttcaaata gtatagaaga tgaagcagca
    37381 taaatcttat aattcatctt cttagtttga tattgtttta aataaagtgc aattgtctta
    37441 ttcagttgcg acttattggt cgtaaaagta cctactttta ctttatcttg taccccagtc
    37501 gaaatttttc ctgtattagg ctcacgtact tgaattaaac gacttgcaat cactgctgtg
    37561 aaggaaaggt taattgccaa caataagaga aatgcccatt tccaaaaatt taaattacgg
    37621 ccagtttttt cttgtttcat ttattttctc cataacggcg ttagacatga tttgatagcc
    37681 aatattattg ggatgaaaat ggtctccagt aaagagagca tcattagtga tacttgcctg
    37741 actatttgat gactctgtaa taccctcttt accatttatt cccttataaa ggcggtcatt
    37801 aattgggaca aaataaacat tttctgaagc atcaactact tcttttgtag ctttattcca
    37861 attatcaata acggtttgca ttttagttaa ttgtggaaag tttaggtaaa aaggattata
    37921 aatgcctaaa acataaatag gcaatttagg attatcttgt cttgcttttg caagaatttc
    37981 tttcaaacgt tccttatatg cttctgctgg tttctcaaag gaatttagtg ataaatgact
    38041 gagctcttta cgaataacag ccaagacatc attaccacca acagttagcg tcaataaatc
    38101 agctttctct aaatcttttt cgatttgagg atctgtcgtc atacgtttta aaatttgttg
    38161 actagtattc ccagacacac cataattaac agaagtcact tggtaagagt atcgattatg
    38221 gagtgattct gatagcagtg ggacaaaacc accttgagag gttgtatccc ccacaccttc
    38281 ggtcagagaa tctccaagag caacatagtt aagtgggata actttctttg ttaggaagtc
    38341 tttttttgtt aatttaggat ttgattttgg aatgatcaag gaaaaaatcc caaaagataa
    38401 aagaagactt accagaaaaa aagaaagtcc ggtaagtatt tttttcttat tcataacgaa
    38461 ccataacagc aaatgcatcc tcaccagtat gagtttggat gattgagcca gtttctaata
    38521 cggatatagg agcacctaga acagctagtt tttctttaaa gttattagcc atatcagctt
    38581 taccacagta ggagatgcca atttcagcaa tttttcgtgt ttgggcactt tcaacaaaat
    38641 tatctaacca tttgctgaag gtctttagac cgcgaccttt aacaatcggt actaattcat
    38701 ggtttgttag ctccataatt acttttatgt taagcaatga acttaggaga cctgttacac
    38761 gtccaatacg accacctttt actaaatttt caagggtcga tacgccaata aataattctg
    38821 acttctggcg tacttcttcc acacgagcca aaattgtatc taaatcagct ccctctttag
    38881 ctaatttcgc ggcttctaca acctggaatt tttgacactg gtctgtaaaa gtagaatcaa
    38941 taactgtaac atctgcacca gcaatattag ctccctggcg tgatgcttca atagttcctg
    39001 atagtgtatg cgttaaatga attgcaatga tatgttccgc accttcattc attaattttt
    39061 cataaatttc tgcaaaaaca cctacaggtg gttgacttgt ttttggcaat tccttacttc
    39121 cacgcattag gttcaagaat tccccttgtg cttttaagtc attgtctgaa tacaaggtac
    39181 catctatcat aactgacaag ggaacaactg taatgtcaag ctctttaata agttctggtt
    39241 caatggtaat ggaagaatcc gttacaattt tgattttact catatcctat cctattctat
    39301 actttaatat ttcaataccc ttattataac aaaatttaag tgtatcaaca ttaatttctc
    39361 ctaacaatgt tagcttttta aagacgttac caaagctaac taccaagtaa ttctttagct
    39421 tgagtacgtg cagtatcagt tacattgttt cctgccaaca ttttagcgat ttcttctaca
    39481 cgttcttcat agcttaaaag ccttaccgtt gaaactgttg aagagtcact ggaaattttt
    39541 tcaataaaat attggtaatc cgctattgca ataacctgag ctaaatgaga aattgctaaa
    39601 acttgtccat gactaccaat cttgtgaatt ttttgagcaa tagcctgggc cacgcgacca
    39661 gaaactcctg tatccacctc atcaaataca atggaagttt tatcttcctt acgtgaaaaa
    39721 gcagatttaa tagctaacat caatcgagat aactctcctc ctgaagccac ttttaccaat
    39781 ggcttaaagc cctctccggg attcgttgaa atataaaatt caacgatttc gttcccttct
    39841 ttattaaatt ttccttttgt aaattgaact tggaaatcag ctttctccat ataaagctct
    39901 gttaattctt gtttgatttc attttccaat tgtttagcta gcttatgacg ctctaaactt
    39961 aattgatttg ctgattcaat caaatcgtgt tctaaaatct ttaattcttt ttctaaagca
    40021 tctgaggact cttcactacc tgtcaataga ctatattctt tagtaatgtt atcaaaatag
    40081 tctaaaacat cattcacatc accgccatat tttcgcgtta ttgtgttaat aacatctagt
    40141 ctattttcaa tttcttgtag taaaccagcg tcaaaatcta aatcatcgat aacgtcacct
    40201 aaacgtttag taacttcttc aataacgtag taagcttctg aaagattggt ggaaagatct
    40261 ttatattctc gatcaaattc ttctaaagcc ataaggtcat tcattgcaga acgaacattc
    40321 gataaacttg aaaactcttc gttatctaac ataagatatg catttgtcaa agtatctgca
    40381 atattcttat gattcattaa tttatcacgt tgcttgagta gcgtttggtc ttcatctgat
    40441 ttaagggcta cagactcaat ttctgctatt tgaaattcta gcatttcaat acgtgattta
    40501 ttctcttgtt catttttttg cttatccaat acacgtttac gaagctgacg ataagcatca
    40561 aaaagacttt gataacgctc ttttatgaca ttaaattctg tattaccaaa ttcatctaac
    40621 atcagaatgt gcatgttagg tttcattagt tcctcttggt catgctgacc atgaatgtct
    40681 accaaataat gccccacagc ttttagtgtt gacaaattca ccatttgccc attaattcga
    40741 ctaacgctgc gcccattttg aaagatttct cggcgaataa ttaattcatc tgctaattca
    40801 atgccatttt cttccaataa ttggactaat gattgatttt tttcaataga gaaaaatcct
    40861 tcaatttctg ctttgttagc accatggcga atcacttcaa cactagcacg ggctcctaac
    40921 atcatattca tagcatcaat aatgatagat ttccctgcac cagtctcacc agttaaaaca
    40981 gtcattcctg tttcaaaatt aagtgaaatt tcttcgataa tagcgaaatt cttaatagaa
    41041 atttctaaaa gcataaggtc ctctactaat cttttttctc caacattgca atcattgatt
    41101 gacgaatgtg atcagcatct gcttctgatt ttgcaatcaa aaggagacta ttatcgtcag
    41161 ctgtcaaact aaaaatatgt tcttgacaat gtgacattat gaaggtttta attaattgac
    41221 tgttacctgg tatgacatta atattgataa attgctctaa acctagtagc ttatctgata
    41281 ttgataaaat acttgtttta ataggctttg ccacagcagt tgtaaagata gggtcgtttt
    41341 catttgacaa accgtaaata tagcgacctt ttgctgatgg cacttttata atgccaattt
    41401 cattcatatc acgtgaaata gttgcttgag ttagagttac cccataagct tccaatctgc
    41461 gtaacaactc atgttgtgtt tcaacagcat ggttaagaac aatttgctta attaaattta
    41521 aacgttcact cttcttcatt tttcttaaat tccttatgtg ctttttctat aacatcttgt
    41581 atttggtcaa gcacaagatt ttgtggatct tgacactttt gcaaatgcat taaaaactca
    41641 atatttccat gtccaccttg aacgggcgaa aaatcaagat gtttaaccgt atatccataa
    41701 tctttcgtga aattggtcac tgttgtcaaa accttttcat gaaccaactt gtctttgaca
    41761 ataccatttt taccaatttg ctcacgacct gcttcaaatt gtggtttaat taatgccact
    41821 acttgtccac catccactaa aatttctttt agagctggta aaatcaaatt aagagagata
    41881 aatgagacat ctatcgatgc aaattcaggc agtccctcct tgaaatcttc tttttgggca
    41941 tacctaaaat tatattgttc catagaacga acacgatgat cctgacgtaa cttccaaact
    42001 aattgatttg ttcctacatc tactgcgtaa actaaacgcg ctcctgattg tagcataaca
    42061 tcagtaaaac cacccgtaga ggcgccaata tctatagtta gcttatctgc aactgaaatt
    42121 tcaaaaactt gtaaagcttt ttctaatttc aatccacctc tactaacata ttttagtttt
    42181 tcacctttta gttttaattc agtatcgtct gcaacctttt cgcctggttt atcataacgt
    42241 tctccattga taacgttaat caccagtcct gccataacac cacgtttcgc ttgctctcgt
    42301 gtatcaaaaa gtccctgttt ataggcaaga acatctaccc tctctttagc cattcaaccg
    42361 taatccctct attatatcta ttatttttct agcattaaag ttagcttttt tctctagatt
    42421 ttgaaaaatt gcctgagctt ttttcaaagt atcgtcaagt atttcctgtg acttatccaa
    42481 acccaataaa tttggataag ttgtcttttc tgctacaatg tctttatttg gtgtcttacc
    42541 caattcttca aaactagcag tcacatctaa aatatcatca cgtacttgaa aagcatgacc
    42601 gattaaaagg ccagcttgat gcagttttcc ttttacttct tccgacttct cagctaaaat
    42661 acctgctgca acaaagggat aagctaacag gcgtcccgtt ttattaatat gaatcaagct
    42721 taaatcgtca atagataaaa ctttgttttc tcccttcata tctaacattt gtccaccaac
    42781 catgccaaaa ctacccgcag acatagacaa taactctatt aacctaacag taacatcagc
    42841 tttaaaacca gcttttacaa ctaaatcaaa tgggtctaaa aaaagggaat ctccagctaa
    42901 aaccgctgta gcttcatcaa actttttatg gtttgttaag cggccacgac gaaaatcatc
    42961 attatccatc gccggtaaat catcatgaat taaagagcca gtgtgaatca tctccaaagc
    43021 agcagccaca tcataatgac catctattag ttcaactcca aatccctcta aaatttctaa
    43081 aaggatgcgt ggacgaatac gtttcccacc tccatcaact gagtagagga tagccttaat
    43141 taaatcagga gaaacgacac tatgtgtctg tttgtaatga cgatgaatag cttcatcaat
    43201 tttttcaata gtaaccatca agtatccatt tctacttccg ttccgtcagc ttgcatcact
    43261 tttacaagcg tctcttcagc ttcttttaaa gttttttgta actcttttga aataagcatt
    43321 cctttttgaa attcagcaat ggcatcttct aaggcaacat cacctgtttc taaacgtgag
    43381 acaattgtct ctaattcctg caaattttct tcaaatgttt tcttgtctga catccttcac
    43441 ctcaacttct aaaagaccat cttgcatctt aacttgcaaa tgatctcctt catttattcc
    43501 attagtagta gatactaaag tatggttttt ttcgataata gcgtaaccac gcgctacaat
    43561 tctagaacta tccaatgata taagcgcatc ttgagccttt tcaaaacgtg ctagcttgct
    43621 gtcatactga ctagtcattg tacttgataa tagtcgtcta ctttgcacaa cacgctcttg
    43681 gtaagtatga agccgctgtt ttaaatctat gccttgcagt ttttgatgaa gcaaaccttg
    43741 cttttgtctt actgtttgta gtttatcacg catagaatat gttaactgct gatttaggtt
    43801 atctaatttt tgaaggaaac catcatatag acgctctggt tgcctaaaaa taactgattg
    43861 ctttgatttt tgcaacctct cctcttttgt cctgataaga cgaaggctag actgatacat
    43921 tctattttca cgttcagtaa tccaagacaa gatatcaatt ttagtaaccg ttgtagcaag
    43981 ctcagctgct gcagttggag ttgctgctct gcgatcggct acaaaatctg ctaaagttgt
    44041 atcagtttca tgtccgacac tagaaataac gggaagacga gattcaaaaa tagcttctac
    44101 aacacattct tcattaaatg cccataagtc ctcaatagaa cctcccccac ggccaacaat
    44161 taatagatcc aaatcctttt tctcgttagc taaagcaata gtttgtgcta tctcctgagc
    44221 tgcgccctct ccttgaactt ttgtggggaa aagtaatatt tccactcctg ggaatcgacg
    44281 agatactgtt gtaataatat cccgaataac agctccactt ggactagtca caactcctat
    44341 ttttctaaca aattgaggaa gtaattgctt atgacgatca tcaaagtaac ctgcttgaga
    44401 gagtttcttc tttagttgtt caaattggat agcaagagct cctataccat ccggttctgc
    44461 tttttcaaca ataatagagt aggagccgct tggttcataa agttgaacac ggccaactac
    44521 attaaccttc atcccttcct ctaattcaaa acccagtttt ttgaattgac ctgaccacat
    44581 tgtcgcctga atgactgatt tatcatcttt taaagagaag tattgatggt ttgggcgacg
    44641 acgaaaatta gacacttgcc cagttaaata aaccctctct aaataagggt ctttgtcaaa
    44701 ttttaatttt aaatacttag tcagtgtcga cacggataaa tagtctgaca tagcacctcc
    44761 tcatgagagt tttattaact tcattatacc aaaaaaactt aaaaaatacc tatttgctct
    44821 acaaatagct cgataaagca aaagaagtgt catgctaaca cctctctact tattttttat
    44881 ttagatagtc tagccatttc ttttggaatc tcatcactaa tcgttgtcgg tagaacaacg
    44941 taggcttctt tagataattc ttgagcaatt gccgaatgta gataaactcc cactgatacc
    45001 ttatctagag gagaggcttc tttaaattgc gctaacatac ctgcaatcat accacacaaa
    45061 gtatccccca tccctccagt cgcctggtaa ggacctccta ctataatttc tttttcgtct
    45121 aaatcttgaa aaatacgcgt atgcgaactc ttagcgacta agatcgtccc tttgggaaaa
    45181 gatttaagag cggtttgggt attttctttt gtctgttgcg atacagcaat acctgacaat
    45241 cgctcccatt ctttttgatg aggtgtcaac actaaattct tagcctttgt ttgcggaaac
    45301 gcaccttctg ataacagagt caaggctgat ccgtcaatga tgacactttg atgtgattga
    45361 atcttctcca tggtccggtt caaaattttt cgagatgatt ctgatactcc taatccagga
    45421 ccaattacaa taacatcact ttgaacaatt gatttttcca accatttgta atcatcacta
    45481 tcaaacgcca ttacctctgg tagttgacta tgcaaagagg ggatattgca actttgggtt
    45541 gctacagtaa ctaatcctgc accagttttg acacaggcca aagctgccat tataatagca
    45601 cctccatagg gataaaaacc tcctatcaga agaacactac cataatcacc tttatggctt
    45661 gatttagggc gtggcttaat taacgccctc gcttcttgtt caccaacaat catagactca
    45721 cacttctaag agctgcttga taagtttgtt ccaaaagcat tgtaatcgtc ataggtccta
    45781 cgcccccagg aacaggtgtt atcatactag caacttctgc cacttgttca aatacaacgt
    45841 ctccaatcaa tttaccattt tcatcgcgat tcataccaac atcaatcaca acagcacctt
    45901 ctttaacgaa gtcttttgta acaaagtgcc cctgaccaat cgcaacaata aggatatcag
    45961 cctcctttgt tacctcagaa aggtttcgag ttcttgaatg tgtcaaagta actgttgcgt
    46021 ttttatcaag gagaagttgt gccattggct taccaacaat attggacctt ccgatgataa
    46081 cagcatgttt tccctctaaa tccacatggt actctctaaa catctccata ataccagctg
    46141 gcgtacaagg taccatcatc ggtcgtcctg accaaagatg acccgtattc attgggtgga
    46201 aaccatcaac atctttctta ggatctattg ctaaaataat ttttttatcg ttaatatgtt
    46261 gtggtaacgg aagttgaact aaaataccat ggatactttt atcttcattg tattgatgga
    46321 taatatcaat cagttcttct tgagaaatgg attcagataa tcgtaaagtc tcacttttaa
    46381 aaccagcttc taatgcagag cgttctttat ttcgaacgta cacttggcta gctggattat
    46441 ccccaacaag gattacagct aaaccaggta tgataccatg ctgttccttt aacctttcaa
    46501 cttttcgccc taactcagcc tgcatttttt gtgataaggc tttaccatct attaattcag
    46561 tcattgatac tccttcttca tccgtttttt ctatattata tcaaaaaata gcgattatgc
    46621 tattttatat cgctattcat tttgtatcaa ttttaatcaa tagaaaaggc aatgaatccc
    46681 tcatcgcctt tttacatatg acttatttag tactattcat tttagcacga caagccgact
    46741 caattgcatt aagtttcgat aaactatctg cttctgtaca accaatcgta taaaggtaac
    46801 attttatctt aggttctgtc cctgacggcc ttaaagcata ccatgaaccc tcattaaaat
    46861 aatattttaa acaattttgc tttggaaaat ccttataacc atccttgaaa tcaatagaat
    46921 tctccaatgt catctcacct acttgtaata ttgggttctg tctaaaatcc tccataatac
    46981 gactaatacg ttcttgcccc tcagcaccct ctaactcaag agaaaattgg cgctcgttgt
    47041 aatagccaaa ttcatcgtaa atggtttgca aaacgtctaa aagtgtttgc cctcgttctt
    47101 tataataggc agtcatttct actaccatca ttgaagcact cacagcatct ttatcacgta
    47161 caaaagtgcc atagcaaaaa ccaatacttt cttcatagcc aaagagataa gttttatcct
    47221 ttgagatatc atattcatta gcttttccac aaatattttt aaatcctgtt aaagtttcaa
    47281 cagtttcaat attatattta tctgcaataa cttttgatag atcaccagtt acaatggatt
    47341 ttaccaatac aggatgatgt ggcaaattcc ctaaggcaca tcgttgtgaa aaaatataat
    47401 aggaaagaag tgcccctatc ttattaccat ttaagaaaat atattctccc ttactatctt
    47461 tgacttccaa tgctactcgg tcacaatctg gatctgtggc aagtaagaca tctgcatcaa
    47521 ctgactttcc tagagattct gaataggcaa atgctttagg aacttcaggg tttggatagc
    47581 caactgttgt aaaatcagga tcgggcattt cctgctcagg tactacataa atattttcaa
    47641 aaccacggcg tcttaaaact tcgcgcacag gtaaatttcc tacgccattt aaaggagtat
    47701 aaactactcg gactgactta tcaatgttag tatcattaat agttaaacca agcacttctt
    47761 ttttatatgc ttcttcaata ctctcatcaa tataacttgc caaaccactt gccagagcct
    47821 cttcaaaggg tatttgctta atttgctgat aatcggttat agcatccata tgattggcaa
    47881 tttggtcagc aatatcatct aaaatctgag atccttcttt ccaatatgcc ttataaccat
    47941 tataagcttg aggattatga ctagcagtaa ccatcacacc cgaaacacat cctagagcac
    48001 gaatagcata tgagcacatt ggtgttgggc gaatcccttt ataaatataa gacttaatac
    48061 catttgctgc cataatggaa caagttaatt ctgcaaattc cttagattga taacggacat
    48121 cataactaac tgcaattcca cgtgcaatag cttcagggcc atgatcaata atcgtattag
    48181 ctaatgcttg tgctgctttc cccaccatat aagtattcat acgattggtt cctgctccaa
    48241 gtttacctct caatcccgcc gttccaaatt ctaatgtttt gtaaaaacga tcctgaattt
    48301 cagattcatc tcctttaata gcttctaaat ctgacttaat atctttaccg agtgagtcgt
    48361 tttctaacca ctcttgataa atctctttat aattcatatg agacatccta ttctaagacc
    48421 aaaatggtaa aaatcagctc catgataaga gattttagtt cttcaggagc taactgaata
    48481 gtatcaaatt aaccttataa agtcaactta ttttataaat taactacact taatatattt
    48541 aaagcatcta aatttttttc atcaccctat cattcttaat ctacattata tatagactta
    48601 ccaacttcca aacaatacag taacaattta ctcataacaa tggaataata aaagtctaaa
    48661 acaagttgtt ttagacaatg aaattataat actttactta agaagtctct tgtgcggatt
    48721 tcttttgtct gctcaaatac tttcttaggg gtcccttgct caacaataat ccctgcatcc
    48781 ataaaaatga cacgatccgc tacttcacgt gcaaaaccca tttcatgagt gacaataacc
    48841 atcgtcatac cagatttagc taaatcttgc ataacagtca agacttcacc taccatttca
    48901 ggatcaagag ctgaagtagg ttcatcaaaa agaaggacat caggattcat tgcaagacct
    48961 cttgcaatag caatccgttg ttgttgtcct ccagataagc ttgctggata agcattagcc
    49021 ttctctttga gtccaacttt ttcaagtagc tcgtatgctt ttgtctgagc atcaagctta
    49081 gaaagtccct ttgtcttaat aggtgataaa gtaatatttt ctagtacagt catattggga
    49141 aatagattga actgttgaaa aaccatgccc attttttcgc gcattttaaa aatatcattc
    49201 tttttgtctg ttatatcaat cccttcaaaa gtcactgttc cctttgttgg tacttccaag
    49261 agattcattg ttcttaaaaa tgttgactta ccagagccag aagggccaat aataaccacc
    49321 acttctcctt gatgaatatc caagtcaatg ccttttaaaa cctcattttt cccaaaactt
    49381 ttatgaagtc cttgtattga aataattggc tcaaccatct atttttaccc ctttcccaag
    49441 atatttctcc atttgtttta acaaagctga gagaatcgtt gtcaacatta aatagtaaaa
    49501 tgctgcaaat aataacggtg caactggtga gtaagtagcc gttacaactg attgtgctcc
    49561 gttccataat tccatgacac caatagtttg aaggagagca ctatctttaa taattgtaat
    49621 aaattcattc cctagagcag gtaaaatatt tttaaaagct tggggtaaga taacatagcg
    49681 aagtgtattt ttaggtcgaa tccccaacga gtaagctgct tctatttgtc cagatggtac
    49741 agcctcaatc cctgcacgta caatttccga aatataggca ccactattta aggaaatgat
    49801 aatgatacca ggtaaaagtc gtgtaaaatc taaatctaaa acaccaaagc taattgttgg
    49861 taaattgtta aaatgcatcc atgcgaaagc aatcataatt tgaactacca tcggtgtccc
    49921 acgaaatacc catacataga aattagctaa tattgtgaga aaatgtaaat tagtacgctt
    49981 tactaaagca attaacacgc ctataatagt tccaaaaaaa acaacacatg ttgaaatcat
    50041 aatggttacc atcacaccat aattaaaata ggaccaatat tgtggtaaaa atgaaaaatt
    50101 cattgacatc tcctaaaaaa gtaattgcca atagtctatc acacaaagaa taaaaataca
    50161 agtttaaa
//
LOCUS       DATROY010000004        43706 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.004, whole genome shotgun sequence.
ACCESSION   DATROY010000004 DATROY010000000
VERSION     DATROY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43706
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            27..1766
                     /locus_tag="VBD11_000187"
     CDS             27..1766
                     /locus_tag="VBD11_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953167.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1778..2512
                     /gene="ltdR"
                     /locus_tag="VBD11_000188"
     CDS             1778..2512
                     /gene="ltdR"
                     /locus_tag="VBD11_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6953168.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2668..4152
                     /locus_tag="VBD11_000189"
     CDS             2668..4152
                     /locus_tag="VBD11_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6953169.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
     gene            4354..4926
                     /locus_tag="VBD11_000190"
     CDS             4354..4926
                     /locus_tag="VBD11_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6953170.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            5202..6053
                     /gene="ylqF"
                     /locus_tag="VBD11_000191"
     CDS             5202..6053
                     /gene="ylqF"
                     /locus_tag="VBD11_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6953171.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            6040..6801
                     /locus_tag="VBD11_000192"
     CDS             6040..6801
                     /locus_tag="VBD11_000192"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6953172.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            6822..7370
                     /locus_tag="VBD11_000193"
     CDS             6822..7370
                     /locus_tag="VBD11_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6953173.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
     gene            7610..8572
                     /locus_tag="VBD11_000194"
     CDS             7610..8572
                     /locus_tag="VBD11_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6953174.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            8569..9543
                     /locus_tag="VBD11_000195"
     CDS             8569..9543
                     /locus_tag="VBD11_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6953175.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            9540..10301
                     /locus_tag="VBD11_000196"
     CDS             9540..10301
                     /locus_tag="VBD11_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6953176.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            10363..11391
                     /locus_tag="VBD11_000197"
     CDS             10363..11391
                     /locus_tag="VBD11_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6953177.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            11529..12371
                     /gene="dprA"
                     /locus_tag="VBD11_000198"
     CDS             11529..12371
                     /gene="dprA"
                     /locus_tag="VBD11_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6953178.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            12466..14586
                     /gene="topA"
                     /locus_tag="VBD11_000199"
     CDS             12466..14586
                     /gene="topA"
                     /locus_tag="VBD11_000199"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6953179.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            14723..15424
                     /locus_tag="VBD11_000200"
     CDS             14723..15424
                     /locus_tag="VBD11_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953180.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            15436..18066
                     /locus_tag="VBD11_000201"
     CDS             15436..18066
                     /locus_tag="VBD11_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953181.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
     gene            18172..19050
                     /locus_tag="VBD11_000202"
     CDS             18172..19050
                     /locus_tag="VBD11_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6953182.1"
                     /translation="MRKSIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            19157..19747
                     /locus_tag="VBD11_000203"
     CDS             19157..19747
                     /locus_tag="VBD11_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6953183.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            19781..21052
                     /locus_tag="VBD11_000204"
     CDS             19781..21052
                     /locus_tag="VBD11_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6953184.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            21065..21496
                     /locus_tag="VBD11_000205"
     CDS             21065..21496
                     /locus_tag="VBD11_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6953185.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            21588..22472
                     /gene="truB"
                     /locus_tag="VBD11_000206"
     CDS             21588..22472
                     /gene="truB"
                     /locus_tag="VBD11_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6953186.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            22485..23417
                     /locus_tag="VBD11_000207"
     CDS             22485..23417
                     /locus_tag="VBD11_000207"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6953187.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            23460..23873
                     /locus_tag="VBD11_000208"
     CDS             23460..23873
                     /locus_tag="VBD11_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6953188.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            23866..24147
                     /locus_tag="VBD11_000209"
     CDS             23866..24147
                     /locus_tag="VBD11_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6953189.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            24137..24901
                     /locus_tag="VBD11_000210"
     CDS             24137..24901
                     /locus_tag="VBD11_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6953190.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            24969..26279
                     /locus_tag="VBD11_000211"
     CDS             24969..26279
                     /locus_tag="VBD11_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6953191.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            26458..27324
                     /locus_tag="VBD11_000212"
     CDS             26458..27324
                     /locus_tag="VBD11_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6953192.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            27370..28287
                     /gene="pstC"
                     /locus_tag="VBD11_000213"
     CDS             27370..28287
                     /gene="pstC"
                     /locus_tag="VBD11_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6953193.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            28277..29164
                     /gene="pstA"
                     /locus_tag="VBD11_000214"
     CDS             28277..29164
                     /gene="pstA"
                     /locus_tag="VBD11_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6953194.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            29176..29979
                     /gene="pstB"
                     /locus_tag="VBD11_000215"
     CDS             29176..29979
                     /gene="pstB"
                     /locus_tag="VBD11_000215"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6953195.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            29991..30749
                     /gene="pstB"
                     /locus_tag="VBD11_000216"
     CDS             29991..30749
                     /gene="pstB"
                     /locus_tag="VBD11_000216"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6953196.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            30783..31436
                     /gene="phoU"
                     /locus_tag="VBD11_000217"
     CDS             30783..31436
                     /gene="phoU"
                     /locus_tag="VBD11_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6953197.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            31582..34131
                     /locus_tag="VBD11_000218"
     CDS             31582..34131
                     /locus_tag="VBD11_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6953198.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            34293..34973
                     /locus_tag="VBD11_000219"
     CDS             34293..34973
                     /locus_tag="VBD11_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953199.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            34957..36270
                     /locus_tag="VBD11_000220"
     CDS             34957..36270
                     /locus_tag="VBD11_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6953200.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDILLFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            36359..36691
                     /locus_tag="VBD11_000221"
     CDS             36359..36691
                     /locus_tag="VBD11_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6953201.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            36709..38274
                     /gene="ffh"
                     /locus_tag="VBD11_000222"
     CDS             36709..38274
                     /gene="ffh"
                     /locus_tag="VBD11_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6953202.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            38341..39027
                     /locus_tag="VBD11_000223"
     CDS             38341..39027
                     /locus_tag="VBD11_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6953203.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            39017..39790
                     /locus_tag="VBD11_000224"
     CDS             39017..39790
                     /locus_tag="VBD11_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6953204.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWVLLV"
     gene            complement(39823..41418)
                     /locus_tag="VBD11_000225"
     CDS             complement(39823..41418)
                     /locus_tag="VBD11_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6953205.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
     gene            41803..42873
                     /gene="xerS"
                     /locus_tag="VBD11_000226"
     CDS             41803..42873
                     /gene="xerS"
                     /locus_tag="VBD11_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6953206.1"
                     /translation="MKRELLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKE
                     YRRFLEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVS
                     QTTINRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGNETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDINLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEY
                     RGVPNRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDKL"
     gene            complement(42916..43668)
                     /locus_tag="VBD11_000227"
     CDS             complement(42916..43668)
                     /locus_tag="VBD11_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001899410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953207.1"
                     /translation="MLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVSETIE
                     QTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAFLKFR
                     QYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFALSQVL
                     DNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQKATGM
                     GLYMTKEVLSSLNLSISVDSKINYGTAASIHK"
BASE COUNT        14814 a         7341 c         7792 g        13759 t
ORIGIN      
        1 actaattaat actttggaga gatttaatga cactcttttt aattatgatg gagcgagcgg
       61 ggttaatcat attattagcc tatgcctttg tgcatattcc ctttataaaa caaactttga
      121 agcaacccga attaaaaaaa caccagtata ttctgcttat cttatttagc ttatttgcta
      181 ttatttctaa ttttacaggg gtagaaattc agtcagattt atctatcatt ccacaaactc
      241 ttaatcatat agcagatcaa tcatcagttg ccaacacacg tgttttaacc attggtgtat
      301 caggtctaat cggtggcccc atagtcggta ttatagttgg tctcttatct gtattcgtta
      361 ggtacttgca aggaggatta gcccctcata tttatgtcat ttcttcctta ctaattggtt
      421 tatgctctgg gttatcaggc aattatcttc gtaaaaatta taataaaatt cgtgttttag
      481 atgcaatggt tgttggtttc gggatggaaa ttctacaaat gatctgtatc ctcattttta
      541 gtgttgactt taatcaggcg cttcgattag tatcttttat cagcatgcca atgattttaa
      601 gtaatacttt aggtttaggt atatttatat ccattatttc atctactcaa aaattagaag
      661 aacatgccaa agcatttcaa acccatcaag tacttgaatt agcaaattta acccttcctt
      721 accttagaaa gggactgaca acagaatctt gtcaaccagt agctgaaatt attcataaac
      781 atatggatgt gagtgctgtt agcctgacta gtcaatcagc cattttagcc tatgtaggag
      841 atggagctga ccaccatctt cctaatactc aaatattaac aaagcttgct aaaagagcaa
      901 tagatactgg taaagttagt gtcgcaactg ataaaagtga aatcgaatgc gaccatagga
      961 attgcccact tagttcagca attgttattc cattacacat ccacgatgtt atagtgggta
     1021 ctttgaaatt atatttttca gacgcacaac atatgactta cgttgaccgt caattagctg
     1081 aaggattagg caatatcttt tccactcagt tagctttggg acaagctgag gaagcaacaa
     1141 ggttactaca ggatgcggaa atgaagtctc tccaagcgca ggttaatccg cattttcttt
     1201 ttaatgcact caatactatt tatggtttaa ttcgcatgga tagcgaaaaa gcaagaaagc
     1261 tcgtccaaga ttttagcaag gttattcgtg ctaatcttca acgggcaaag caaaatttaa
     1321 ttcctctcca tgatgaattg gaacaggtta acgcctattt agcccttgaa gaagcaagat
     1381 ttcctaacat ggtggccttt aatttagaca accaaacaaa ttcagatgac aacttaatga
     1441 ttcctccctt tacattgcag gtcttgattg aaaactctta taaacacgct tttaagcata
     1501 tcaataaaaa taaccagcta aaggttacta ttgcaagaaa taatgatagg cttcatatca
     1561 ttgtccagga caatggtatt gggattccaa aagaaaaatt aataacgctt ggaaaaaaaa
     1621 ctcaaatttc aaaaaaaggt tctggtacag ctattgagaa tctcgttcgt cgcttgaata
     1681 ttatttatga tggacaagca agtttaaaat ttgaatcaaa tgattcagga acttgtgcta
     1741 ttgttaacat ccccattaaa aattagaaag gtattgtatg aaagttttag tagttgatga
     1801 tgaaccagtt gcacgtaacg aattaattta ccttcttaat aagtatgatt ctaacctcgt
     1861 tatagcagag gcgcatgata tggctactgc attagctatt ttacttagag aaacttttga
     1921 tgtagcactg ttagatatcc atctcagaga tgattctggg ttgcaattag cagagtatat
     1981 caataaaatg cccaaaccac cattattgat attcgcgact gcttatgatc aatatgctat
     2041 tcaggctttt gagcaggatg cgcgtgatta tttgttaaaa ccctatgagt ttgataggtt
     2101 aaagcaagct atggatagag taaaaggagc gctaagtaca tctacaatta tagagagcgt
     2161 agcttccggt cctctcttca agcaacagta tccattgaca gtagaagatc gaatctatct
     2221 ggtgtcggcg gatgatatcc ttttgattga agctatgcaa ggaaaactga ttatacaaac
     2281 acctgataaa aattatgaaa ttgatggctc tctacaacaa tggcaagata aactaccatc
     2341 atctcaattt gtacgggtac atcgctctta cattgtgaat attaatgcta ttaaaacgat
     2401 tgaaccttgg tttaaccaaa cacttcagtt acacctttgt aataaaataa cagttcctgt
     2461 tagcagagca aatgtaaaac ccctaaaaca aatgttaggc atatctacct aactaatgaa
     2521 aagttattca tcataaaact tagactattc atttctaaaa aacaaccatt catcttgaaa
     2581 ataggaaatt tcaatttttc aatgctatac taaagtcatg aaaacgctta ataaaattaa
     2641 gcaaaaaaat ttttaggagg cagtgatatg gtaacatttt taggaggggt agccctttta
     2701 attgttggtt atttcacgta cggtagatac attgaaaaga attttcaaat tgatgaaaat
     2761 cgccaaacac ctgcagaagc attaagggat ggatatgatt ttgttcctat gccaaaatgg
     2821 aagaatggga tgatcgagtt attgaacatc gcaggaactg gacctatctt tggacctatt
     2881 ttaggagcac tctatggtcc agttgcttat atctggatag tcttaggatg tatttttgca
     2941 ggggctgttc atgattatat gattggaatg atttctctta gaaataatgg tgcatatctt
     3001 cctgaattag ctagtcgcta tcttggaaaa tcaatgaagc atgtgatcaa cattttttca
     3061 atgctattac ttattttagt tgcaaccgta tttgtggtaa ctccagctaa tcttatcctg
     3121 agtattttac cagctgggac actatcttta ccttggataa tcggtcttat tttcgtttat
     3181 taccttattt ctacagtatt acctattgat aaagcacttg gtaaagttta tcctgtattc
     3241 tgcgtcatcc taatggttag tacagcagca gttggcttcc gtttattaac tggtggcttt
     3301 gatatgccaa acttaacttt tgaaaccttt aaaaatatgc atccagctgg acttgggata
     3361 ttccctgctt tattctttac catttcatgt ggagccatct ctggtttcca tgctacccaa
     3421 gcacctatgg tctcaagaac aactgttaat gagagagaag ggcgtttcac tttctatggt
     3481 atgatgattg cagaaggagt aattgcgatg atttgggcag gtgcttcaat gagcttgttc
     3541 aaaggtcaaa atctctatga aatgattgct gctggaacac cttctgcagt tgtcaatcaa
     3601 gtaatgttaa tgttactcgg tagtgtaatt ggaactatcg ctatcattgg tgttattgtt
     3661 ctccctgttt catctggttt atcagctttc cgtagcttac gtactatcgt tgctgattat
     3721 atccatgtca aacaagatac tcttccaaaa atctttgctg taactatccc attatatgtt
     3781 atttcatttg tccttacaca cgttgatttt aatctcctat ggcgttactt taactgggca
     3841 aatcaagtaa ctgctgttat tggtttatta gttgctacaa gatatctcat cctaaaaaga
     3901 cgaaattatt gggtaacatt tgtaccagct atgttcatgt tgtatgcagt agttgtttat
     3961 attttatctc aaccaattgg atttaatatg ggcttaggca tcttaactta ttcacttgct
     4021 ttagtgttaa caggtattct tgttggactt ttctggaaat ctggtcaaaa acaattaaaa
     4081 actgttcatc ctgaagcatt cctgtttaac gatcatagac caattaatta ttacagtagt
     4141 ttagattctt aatattattt tgaagccatg ttattaagac ttacagttat cacaataacc
     4201 tcgaatgaag tatcattcga ggttattttt ctactaaaaa tctcagtaaa tatttctatt
     4261 tgattacaaa tgttacaaaa atatttgaca accttctttc tgaaacttat aatagatttg
     4321 tccataacag agcaaaagag agagggatag tacatgagaa aacgttcaat tttgccaata
     4381 ttagctgtct tatttgtttt catgagtatc tttagcgtat actctatttt tcatggtgat
     4441 aaagctaaag aaaaagcaag tgtcataaaa caagcatctc aaacaagtca aacttcaaaa
     4501 aaagaggtct tacaaaagaa gacttatcca aatctaaata aatattcaaa tctagaaatt
     4561 catgtttcat ctacgaggca aacaatgaca ataacctcga gtgacaaggt catctttaaa
     4621 acgattgttt caacaggagc aaaagagagc cctaccccaa aaggaacttt tgtcatagaa
     4681 ccggaacgag gagatttttt ctataacgcc tcatcaaaag aaggagccta ttattgggtc
     4741 tcctttaaag agcatggtat ttatctcttt catagcgttc cgactgatca acaaggaaat
     4801 gagatcccag aagaagcaaa gcaattagga aaagccgcct cacatggctg tgtgcgtatg
     4861 tcaagggcgg atgcaaaatg gttctatgaa aatattcctc aaggaacgac tgttaccatc
     4921 aaatagtctt aatgtggtaa aaatcatgtt attaaaagct tggagtaaac ttccagagct
     4981 ttttcgtata tcccttaaac tcaattagta aacgaaacct atcagcggaa taaatgtata
     5041 aagtatttcg atatcattaa aaaaatgata gaataatagt gttaatcgaa tattttatcg
     5101 acctttatat gcggttgaag aagaataaaa ctaaaagatt agacctctaa caaatagatg
     5161 tagactttta aggtagtcta tacataagga gaatttattt catggctact attcaatggt
     5221 tcccaggaca catgtccaaa gctcgccgtc aagttcaaga aaatattaag catgttgatt
     5281 ttgttacaat tttagtggat gcaaggttac ctctttctag tcaaaatcca atgctaacca
     5341 aaatcgtcgg agacaaaccc aaactaatga tcctcaataa agctgatcta gcagatccca
     5401 ttcgaactaa agaatggaga gatttttatg aatctcaagg tttaaagaca ttagctatta
     5461 attctaaaga acaatcaaca gttaaaaaag taaccgacat cgctaaaata ttaatgtccg
     5521 ataaaatcgc taaactacgg gaacgtggta ttcaaaaaga gacacttcgt acgatgatta
     5581 ttggaattcc gaatgcaggc aaatcgactc taatgaatcg cctagcaggt aaaaaaattg
     5641 cagtggttgg taataaacca ggtgtcacaa aaggtcagca atggctcaaa tctaataagg
     5701 aactggaaat tttggacact cctggtatct tgtggcccaa atttgaagat gaattagttg
     5761 gattaaagct agcattaaca ggggcaatca aagatcaatt attgcctatg gatgaggtaa
     5821 caatatttgg attgaattac tttaaaacct actatcctga tcgtctcaaa gagcgtttta
     5881 aaagtattaa tttagaggat gaatctcctg aaatcatcat gaccttaaca caaaaattag
     5941 gttatcgtga cgattacgat cgcttttata atttatttgt caaagaagtt cgcgatggta
     6001 aattaggacg ctatacgtta gatatcgtcg gagaacatga tggcaacaat taaagaaatc
     6061 aaagcaattt tagagactat tgttgattta aaagacaaac gttggcaaga atatcaaaca
     6121 gattcacgcg caggtgttca aaaagcaatc cttcaacgaa aaaaaaatat tcagtcacat
     6181 ttagacgaag aagcgcgctt agaacaaatg ttggtctacg aaaaaaaact ttacattgaa
     6241 catataaacc ttattgcagg aatcgatgaa gtaggtagag gtcctttagc aggaccagta
     6301 gtagctgctg ctgttattct tccaccaaac tgtaaaataa aacatctcaa tgactccaaa
     6361 aaaattccaa agaaaaagca tcaagaaatc tatcaaaata ttttagatca agcgttagca
     6421 gttggtattg gtattcaaga cagccaatgt attgatgata tcaacattta tgaagctaca
     6481 aaacatgcta tgattgatgc agtttctcat ttatctgtag ttcctgaaca tcttttaatt
     6541 gatgctatgg ttttggatct accaattcca caaactaaaa ttatcaaagg tgatgctaat
     6601 tcactctcta ttgcagcagc ttcaattgta gctaaagtga ccagagataa aataatgtct
     6661 gattatgata gcaaatatcc tggatttgca ttttctaaaa atgctggcta tggtactaaa
     6721 gagcatttag agggtttgca aaaatacggt attactccga tacatcgtaa aagttttgaa
     6781 cctataaagt caatgttgta aataatacgg aagaggaaaa gatgaccgaa aaagaaaaaa
     6841 tgttagcagg acaatattat cgtccatcag cacctgaact acgcaaggat agagaagttg
     6901 cactaaaaaa tatgcaagcc tttaacaatg aagatgatag tagcaaacgt aacgtcattt
     6961 tacaaaaatg gtttggtgct actgggaaga gtatccatat ggaacaacgt tttgtctgcg
     7021 attatggttg taatatttat gttggtgaaa acttctacgc caattttaac caaactttct
     7081 tagatgtttg tgaaattcgt attggcgata actgtatgtt tggcccaaac tgtcaactcc
     7141 tcaccccact ccatcctcta gatccaatag aaagaaattc aggattagaa tacggtgccc
     7201 caatccaaat cgggaataat gtctggctag gtggaggagt gactatttta ccaggtgttg
     7261 tattaggaga taatgtcgtc gttggcgctg gaagtgtcgt tacgaaatca tttgaaaata
     7321 atgttgttat agcaggaaac cctgctaaga taatcaaaaa attagcttaa tgtaagaaaa
     7381 aacctttaat tataacaaag gttttttcta atttaaaaaa tagataaaga gaatattctg
     7441 ttaatctaat ctatcttatt tataatgatt ttaaatagaa aaaccttgat ataataatgt
     7501 tttcttaagt ataaaattct ttgaaaaagt agatattttt ccaaaaaatt atgttattat
     7561 agatttgtgc tcatttttga acactttatc tttcatttta aggagtttta tgaaaaagct
     7621 attaaccact cctgtaatgg gttgtacttt ggttatactt tctctaacgt ccctatttgt
     7681 tggtgttaaa tctatcccac tggagcaaat aacacacctt aatcaatcac aggtagatat
     7741 ttttttagcg agtcgcttac caagaaccat tagtatccta atatctggtg caagtttgtc
     7801 agtctgtggc ctgctaatgc aacaactcac ccaaaacaag ttcgtttcac cgacaacttc
     7861 agggactatg gattgggcga aattaggagt agttgttacg ctgattttct ttaaaaatac
     7921 ctctattttt attcaactat gtattgcttc aggttttgcc attttaggat cgctgctttt
     7981 tgtaactatt ttaaaaatga ttacttttaa ggataatatt tttatacctc ttatcggact
     8041 gatgcttgga caaattgtag cagcagcaac agtattttta ggaacacatt ttcaagtact
     8101 acaatctgtc aacagttggc ttcaaggtaa tttttccatc atgactagcc atcgctacga
     8161 aattctttac ttagcattgc catgtttgtt tttagtgtat ttttttgctc atcagtttac
     8221 cattgttggg cttggagaaa gcttcgctaa aaatttaggg attgcttatg aaaagatgat
     8281 ttactttggt ttagttttgg tttcaattat gacatcccta gtcattatta tcgtaggagc
     8341 tttacctttc ttaggtttaa tcgtacctaa tctaattagc ttaactaaag gggatcatat
     8401 gtcctcaact atcttagaga catcgctttt aggggcttgt atcgtcatga tatgtgatct
     8461 ttttggtcgc ttagttattt ttccttatga agtctcaata ggagttacgc taggagtatt
     8521 gggtagtgcc tttttcttga tttcaattat aagaaatgag ggaaaatcat gattaaggat
     8581 ttagagatac caaaattact tattttactg atactactaa ttgctaccat tattttgttt
     8641 ttaatttatg gtattccaac tgacgcaaat gaatttttga ttatctatat cctaaaaaca
     8701 cgttatcaaa aattgattgc attaattttg gttggtatct gcattggtag ttctagtctt
     8761 atttttcaaa ctctaaccaa taaccgactt ttaactccgt ccattattgg tttagatagc
     8821 ttatatgttc ttatccaaac aggattaatg tatttaatcg gagctcagcg tgtcataaaa
     8881 ttttcatctt ttagcagctt cctcctttca ttattattaa tggttggatt cgcttactta
     8941 ttatttacga tactttttcg aaataaaaaa cagtctttgt attttgttct attagctgga
     9001 ctcatattta atacattatt ttcttctatc tcatccttta ttcaagccat tatggacccc
     9061 aacgatttta tgattttaca aaatcagctc tttgctagtt tcaatgctat taataccaag
     9121 atcctttgga tttctttcat tatcattgtc gttagctttg tcattaattg gccttttata
     9181 aaggaattgg atgtcttact attgggtaaa gaaaatgcga tatcactcgg catctcatat
     9241 caaaagctta ctactagaat ttttttatgg ttagcattga tggtagctat agcaacagca
     9301 ttagttgggc cgattacttt tttaggctta cttgtagcac atattaccta tcacagtttt
     9361 cataccttta gacatcaaat attagttcct atagccattg ttatctgtat ctttacactt
     9421 gtcttagggc agcatctcgt tcaaaatcta ttacacctca ctgttcaact aagtgttctc
     9481 ctcaacctta tcgggggaag ttattttata ttcaccttaa tcaaaggaag gaaaaactta
     9541 tgattcaaat aaacaattta cataagttct atggccaaaa ggaaatatta aaagacatta
     9601 atatttcgat tcctaaagga aaagtaacag ctattttagg tccaaatgga tctggaaaga
     9661 gtacattgct gagttgtatc agtcgacttg aaccttatga taatggtgaa attttccttg
     9721 ataaagtacc attagcccac tacagttcaa atgacttagc caaaacatta gccatattaa
     9781 gacaatctaa tcacttaaca ctcaagataa aggtgagtga cttgatagga ttcggtagat
     9841 ttccttatag taaaggacgg ttaagtcaaa aagataaagc tgtcatagaa tcagttatat
     9901 cttatatgga tttaaacgat atagctgacg agttcattaa taacctcagc ggaggtcaaa
     9961 ttcaaagagc ttttatcgca atgacaatgg cacaagatac tcaatatatt tgcttagatg
    10021 agccactcaa caatttagat atgaaatatg ctgttcaaat gatggatctt atcaaacgat
    10081 atgcgtatga gtttaataaa actatagtga ttattattca cgacatcaat tttgcaactc
    10141 actacgctga caatgttgtc gctttaaaag aaggacaagt ggtgtcttgt gggactgtcg
    10201 aagatgttat gcaagaaaaa atcctttctc atttatttga tatgcctatc cgtatcgaaa
    10261 ctgttgatgg taaaccaatt atcctttttt gtacaacgta aataaagaga tttatgtagg
    10321 ataacatttt tagtttttat ctattttagg agattttatt taatgacaaa aaaacttatt
    10381 attgctatat tagcactatg cactatctta accacttctc aagctgtttt agctaaagaa
    10441 aaatcacaaa ctgttaccat aaaaaacaac tattcggtct atattaaaaa agaaaaaaga
    10501 gacaagccgg ataataaaaa gcaaatcagc gagacactta aagttccttt aaaacccaaa
    10561 aaagtagttg tttttgatat gggagctttg gatactatca cagctttagg agctgaaaaa
    10621 tctgttattg gtatcccgaa ggctaaaaat gctctaagtt tattgcccaa taacgtcaaa
    10681 tctgtttata aagctaagag ataccaagac gtaggaagtc tcttcgaacc aaactttgaa
    10741 gctattgctc gtatgcaacc tgatgtggtt ttcctaggag cacgtatggc ttctgttgat
    10801 aatattgaaa aattaaagga ggctgcacct aaagcagcat tagtatatgc tggagtcgac
    10861 tcaaaaaaag tatttgacaa aggagttgct gagcgtgtca caatgttagg gaaaatcttc
    10921 gaccaaaata aaaaggcaaa aacctttaat aaagatatcg cacaagctgt tcttaaattg
    10981 cagaaaacta ttgagaaaaa aggtaaacct acagctctat ttgtaatggc aaacagcggt
    11041 gaacttttaa ctcaatcacc ttctggtcgt tttggttgga ttttctctgt aggtggattt
    11101 aaagcagtca atgaaaatga aaaactaagt tcacatggta ctcccgtatc ttatgaatac
    11161 atcgctgaaa aaaatcctaa ctatctcttt gttttagatc gtggagcgac tattggacaa
    11221 ggagcttcat caaaagaact ttttaataac gatgttatta aagcaactga tgctgtcaaa
    11281 aacaaacgtg ttcatgaggt agatggaaaa gattggtata tcaattcagg cggaagccga
    11341 gtaacactcc gtatgattaa agatgtacag aactttgttg ataatcgtta attttttgtc
    11401 aaaggctagg aaaaatccta gtcttatttt tttgaaaaaa tagctattat caatgcttca
    11461 gagtatagat tgatcttatc actttatttt gtctattatt ttcgaatata gtcaatggag
    11521 gtaaccttat gaatcatttt gaactattta agcttaaaaa agctggacta acaaatctta
    11581 atatcaacaa tattatcaac tatctcaaaa agaatagttt aacttctcta tctgttcgca
    11641 atatggccgt agtatcaaaa tgtaaaaatc ctactttctt tatagaaaat tataaacagc
    11701 tagaccttaa aaaacttcga caagaattca aaaaatttcc agttctatcg attttggatt
    11761 ctaactatcc tttagagtta aaagaaatat ataatccacc agttctactt ttttatcagg
    11821 gtaatattga acttctatct aaacctaaat tagctgtagt gggggcaaga caggcatctc
    11881 agataggttg tcagtctgtt aaaaagatta tcaaggagac taacaatcaa tttgttatcg
    11941 taagtggttt agcgcgtggc attgatacag cagcacatgt tagtgcttta aaaaatggcg
    12001 gcagtagtat agctgttatc gggagtggtt tagatgttta ttatccaacg gagaataaga
    12061 aacttcaaga atatatgtca tataatcatc tcgtattatc agaatatttt accggagaac
    12121 aacccttgaa atttcatttc cccgaacgta accgtattat tgcagggctg tgtcaaggta
    12181 ttgtggttgc cgaagctaag atgagatctg gaagtttaat tacctgtgaa agagcattag
    12241 aagagggacg agaagttttt gccattcccg gaaatattat cgatggcaaa tcagatggat
    12301 gccaccatct tatccaagaa ggagctaaat gcattatttc aggaaaagat atcctttctg
    12361 aatatcagta gtgttttgct ttctactata ggagaagttt tctattgaca gtttaagaaa
    12421 agtgagttat tatgctataa gtttacgata tagaaagtga tacctatggc aactacgaca
    12481 aaaacgtcga ccaaaaaaac aagtaaaaaa aaatcagcta ctgctaaaaa aaatctagtt
    12541 attgtggagt ctcctgcaaa agcaaagact atcgaaaaat acttaggacg taactataaa
    12601 gttgtagcct cagtaggtca tatccgtgat ttaaaaaagt caagtatgtc cattgatttt
    12661 gagaataatt acgaaccaca atatattaat attcgtggta aaggacctct cattaatgat
    12721 ttaaaaaaag aagctaaaaa ggctaaaaaa gtttacctcg cgagtgaccc ggaccgtgaa
    12781 ggagaagcta tttcctggca tttagcgcat attttagatt tagacaaaga agacagaaac
    12841 cgtgttgttt tcaatgaaat cacaaaagat gctgttaaaa atgcttttgt ggaacctcgt
    12901 cagattaaca tggatctcgt tgatgctcag caagcacgac gtgttttaga tcgcattgtt
    12961 ggttattcta tctcacctat tttatggaaa aaagtaaaaa aagggctatc agctggacgt
    13021 gtacaatcag ttgctcttaa actaatcatt gatcgtgaaa atgagattaa ggctttccaa
    13081 ccggaggagt attggactat tgacggttcc tttaaaaaag gaacacgtaa attcaatgcc
    13141 actttctacg gtttagacgg aaagaaattc aaattatcga ataatgaaga cgtcaaaaca
    13201 gttctaaaac gtattaaaac tgatgaattc ttagttgaaa aagttgaaaa aaaagagcgt
    13261 cgtcgtaatg caccattacc gtatacaact tcttcattgc aacaagatgc agctaataaa
    13321 atcaattttc gaactcgtaa aaccatgatg attgcgcaac agctgtacga aggacttagc
    13381 ttaggcacag caggtcatca aggtctgatt acctatatgc gtactgattc tacacgtatt
    13441 agtccgttag cacaaaatga agcaacagaa tttattacta accgttttgg tgcaaattat
    13501 tctaaacatg ggaataaagt taaaaatgct tctggagcac aagacgctca cgaagctatt
    13561 cgcccatcta gtgttaatca tacacccgaa agtattgcta agtatttaga caaagatcaa
    13621 ctaaaacttt acactcttat ctggaaccgt tttattgcaa gccaaatgac agcagcagtc
    13681 tttgacacaa tgaaagttaa tttaacgcaa aatggtgtta cttttattgc taatggtagc
    13741 caagtcaagt ttgatggtta catggctgtt tataacgata ctgacaaaaa taaaatgtta
    13801 ccagatatgg aggaaggaga aagtgttaaa aaggttaata cgaatcctga acaacacttc
    13861 actcaacctc ctgcaaggtt ttcagaagca agtctcatta aaacacttga agaaaacggt
    13921 gtaggtcgtc cttcaactta tgccccaacg cttgagacta ttcaaaaacg ttattatgtt
    13981 aagcttgcag ctaaacgctt cgaaccaact gaacttggtg aaattgttaa tagccttatt
    14041 gtagaatttt tcccagatat tgttgatgtt accttcactg ccgaaatgga agggaaacta
    14101 gacgaagttg agattggtaa agagcagtgg caaaagatta tcgatgaatt ttataaacca
    14161 tttgaaaaag aacttgcaaa agcagaaact gaaatggaga aaattcaaat aaaggatgaa
    14221 cctgctggat ttgattgcga gctatgtgga tcaccaatgg taataaaact aggacgttat
    14281 ggaaagtttt atgcgtgtag caatttccct gaatgtcata acacaaaagc tatcactaag
    14341 gaaataggtg ttatttgtcc tatctgtcaa aaaggacaag ttattgagcg aaaaacaaaa
    14401 cgcaatcgta tcttttatgg ctgtgaccgc tacccagaat gtgagtttac atcttgggac
    14461 aaacctattg gacgaacttg ccccaaatca aatgatttct tagtggagaa aaaagtacgc
    14521 ggtggtggta aacaagttgt ttgctcaaat gaaaaatgcg actaccaaga agagaaaatt
    14581 aaataaagtg gaatctgagt gtttagctca gatttttaaa aaaattatta ttttacataa
    14641 cttaatttat gtatttattt ttatgggaga attaataaaa aagtgctata atataggtgt
    14701 attcatagtg tgaggtgccg ttatgtccta tattgaaatg aagcattcct ataagcgtta
    14761 tcaaacaggt gaaaccgaaa ttgttgctaa caatgatatt agtttcagta ttgaaagagg
    14821 tgagttggtg gttattcttg gtgcatccgg cgctggcaaa tctactgttc tcaatatact
    14881 aggagggatg gatagtaata gtgaaggtga agttttaatt gatggtaaga atattgctaa
    14941 ttataccatc agggaattga ctcgttaccg tagatatgat gtcgggtttg tttttcaatt
    15001 ctataactta gtccctaatc taacagcact agagaatgta gaattggctt cagaaattgt
    15061 tccaaaggca ttagatgccc aacaggcatt agaaaacgta ggtttgggac accgtatcaa
    15121 tcattttcca gcacagcttt ccggtggcga acagcaacgc gttgcaatag cacgagctat
    15181 tgccaaaaaa cctaaactgt tactttgtga tgaacctact ggtgctttag attaccaaac
    15241 tggaaaacaa gtactagcca tcctgcaaaa gatggcgcaa tcaaaagaaa caactgtgat
    15301 cattgttact cataatactg ccttagcgcc aattgcaaat cgcgtgatcc atatgcatga
    15361 ttcaaaaatc agtgatattg taataaacga gaatccaagt gatattcaga acatagaata
    15421 ttagaggtta tacagatggg aaagacattt tggaaagata tctatcgatc aattacgact
    15481 tcaaaaggta gattttcatc aatcctctta cttatgatgt taggctcatt tgcttttata
    15541 ggactgaagg tctcagcacc taatatgcaa agaacagcac aaaattacct tgctcatcat
    15601 catgttatgg atattaccgt tttcaattct tggggacttg acaaacatga tcaaactgtt
    15661 ttagaaagct taaaaggatc acaagttgaa ttttcttact ttgtagatac aacccctcaa
    15721 cagaatagta aatcttatcg attgtattcc aatactaaaa cgatatcaac ctttgatctt
    15781 gttaagggac gtctcccttt aaataaatca gaaattgctc tctcatttca ggaacgaaaa
    15841 aaatatgcaa taggtgataa aattaatttt aaacaagata aaaacaaact gttttcaaat
    15901 acaggtcctc taactattgt aggttttgtt aattctaccg agatttggtc taagacaaac
    15961 cttggtagtt ctcaaacagg ggacggcgac ttagatagtt atggcgtatt agataagaca
    16021 gcatttcact ctccagtata tacaatggca agggtaactt ttaaagatct taggttgatt
    16081 aaccctttct caataagtta caaagaaaaa gtagcgaaat accaagaaaa ggtatcgcga
    16141 aaattaaata ttcacaataa aataagatat accaaaacaa aaaaagagag cctacgtaaa
    16201 atcgatgagg aggaaaaaag cctactaaaa gctcaaaaac aaatcaatcg gttagataat
    16261 gatagcctag cgatgccact atctcaaaga caagctattc aaatgaaaat taaacaagac
    16321 cgactatctc ttttgaaacg aacaaaagag cttctaaaat tgagacataa cacccaaata
    16381 atggaatcgc ctcaaattat tgtctacaac cgtactacct ttcctggagg acaaggatat
    16441 aacacttttg actccagtac aaatagcact tctaaaatca gtaatctttt ccccattatt
    16501 ttatatttag tcgcagcatt agtaacctta acaacaatga ctaggttcgt cgaggaagaa
    16561 agaaccaacg caggcatatt aaaagcacta ggctatagtg accgccaagt tatctttaaa
    16621 tttattattt atggttttat agcaggaaca ttagggacta ctttgggtat cattggaggg
    16681 cattatcttt tacctcgtat tatttctgat atcatttcca aagacctgac tattccaaac
    16741 acacagtatc atcttttctt aaattacagt ttactagcct ttgttttttc attattaagc
    16801 attgttcttc cagtttttgt tatcactcga cgtgagttga aagaaaaagc agcattttta
    16861 ttattaccaa aaccaccagc taaaggatct aaaatagctt tagagtatat caattggatt
    16921 tggaaaaaac tgtccttcac acaaaaagta actgctagaa atatttttcg ttacaaacaa
    16981 aggatgatta tgaccatatt tggagttgct ggatcagtag cgctcttatt ttcaggtcta
    17041 ggaatacaat cctccttaaa gcaaaccgtc aatgaacatt tcggtcgtat tatgccctat
    17101 gatatattgc taacatataa cacaaatgct tctcccccaa aaatacttga actactttca
    17161 aaagattcaa aaatagacaa ataccaacca atacaccttg aaaatcttga cgaatccatt
    17221 cctggacaaa ttaataaaca gtcaatttcc ctattcataa cagacaaaaa acaattacta
    17281 ccttttattt atttacaaga agcaacaact aataagtcat tgcacttgaa taataaaggg
    17341 attattattt ctaaaaaatt agctcaattt tatcatgtta ataccggtga ttttatccat
    17401 ttatctcatt cacaaacact tccttctaga aaattaaaaa taacaggagt tgtcaatgcg
    17461 aatgttggtc actatatttt tatgacaaaa caatactatc gaactatttt taagaaagaa
    17521 gctaaagata acgctttttt ggttaagtta accaaacata aaatcgcaaa taacttagca
    17581 gaaaaacttt tagaaattaa tggagttgag tctcttacgc aaaatgctct tcagctggct
    17641 agtgtagaag ccgttgtgcg ttctttagat ggctctatga ctattttagt tgtcgtatct
    17701 ttattgttag ccattgtaat tctctataat ctcactaata ttaatttagc agaacgtaag
    17761 cgggagctat caactataaa ggtattaggt ttttataatg aagaagtaac tttatacatt
    17821 taccgcgaaa ccattatttt atcaaccatc ggtgtgattc taggcaccat tagcggtact
    17881 tatttacacc gtcaaatgat gttgctaatt ggttcagatc aaatactttt tggtgaaaaa
    17941 gtatcaccaa ctacatttat aataccaata agcgtagtag tcatcattct aataagtcta
    18001 ggttttatag ttaaccatca attaaaaaaa ctcaatatgt tagatgcctt gaaatcagta
    18061 gattagttta taaaaaagat tatcttttct aatcttttcg ataacgattg acagcaagta
    18121 atcttaattg tataatataa cagtataaag ttagataaaa gtgagatatt tatgagaaag
    18181 tctatttttg aggaaactgc aaaaggtaaa gtatcaaggc atgtcctttt aactatatta
    18241 ttggctttga tattactatt tacagcagat ttccttacct atattattat tgaattgatt
    18301 atctcgcttg atttttggaa actagagtgg gatagcgata tcgtccagtt aatcatttta
    18361 ctagcaactt caattagtac ggtaatgtta tttatgtggg tgaaatttaa agagaagcgt
    18421 ccaattagtt ctctagggct atttaaagaa ggagccctaa cagaattatc tagaggatgg
    18481 ttagtcggga gcgttctctt tggagcctgc ttaatattaa caataatttc aggctctgga
    18541 gttatcgaaa ctgttcgttt tacagctgat aaattgattt ggtttcttat ttttgctgtt
    18601 ggatggcagg tacaaagcgc atctgttgaa atattgtgtc gtggatggtt acttccggtg
    18661 agtgccacaa agtatagtaa gatagtttct gtcagtatca gttcaatctt tttcggtctt
    18721 ctgcatagcg ctaataatca tgtaagcctt atctcgattt tcaatctgtg tctctttgga
    18781 ctatttctat cgctctatgt tattttaaaa ggtaacatct ggggggcgtg tggtattcat
    18841 ggcgcctgga actgtgtaca agggagtgtc tttggaatcg aagttagtgg agagccaatg
    18901 ttgtctaact ccctagtcca tgttaaaacc tatggtgctg actggattag cggtggaaaa
    18961 tttggtgtgg aaggaagcat gattaccagt attgttctta tcgtagcatg ttattggctt
    19021 tatcaaaaat cgaattttcc ttacgactaa tagatttaat ctaaataagc ttctcttact
    19081 gaaagcttat tttttatttt agtattaaaa aactatgttt tgtgatataa tattttaaag
    19141 acgaaaggag tctattgtgc cacaaaatcc aacaagtaac ccagagaaaa aaacttattt
    19201 attatctgaa ttggtagact gctttaaagg taaagctgtg ccttcaaaag cagaagcagg
    19261 tgatatccgt attattaatt tgagtgatat gtctccttta ggaattgact accataactt
    19321 aagaacattc caagatgaac aacgatcgct tttaaaatat ctacttcagg aaggcgatgt
    19381 tttaatcgct tcaaaaggaa ctgtcaagaa agttgctatt tttgaagagc aagattaccc
    19441 tgtcgttgcc tctgctaata ttacaatact aaggcctaca caacacatta gagggtacta
    19501 cttaaagctt ttttttgatt cagaagaagg tcaacaggca cttgagaatg ctaacaaagg
    19561 aaaagctgtg atgaatatta gcacaaagga actgttaaat attgcaatac caagtatccc
    19621 gctttttaga caggattatc ttatccaacg ttataaacag gggctcaacg attacaaacg
    19681 taagatagca cgtgcagagc aggagtggga acgtatccaa aatgacatca gacaacaact
    19741 tagctagaac caaatacaaa acatcatagg aagagttatc atgaacgaaa tcaaatgccc
    19801 tcattgtgga acagcttttg ccatcaacga gtctgaatac catcaattac tagaacaaat
    19861 tcgtggagat gcttttgaca aagaagtaag tgaacggttg gaaaaagaac gtctaatatt
    19921 aggggagcaa gcaaaaaatc aattacagga agttgttgta gaaaaagaca aggagatagc
    19981 taaacttcag tacaaagtca aacaatttct tatagaaaaa gacaatcttc tcaaagacaa
    20041 tgagtaccaa ctcgctgagc aattaaatca aaaagacatg atgcttcgcg accttgaaaa
    20101 ccaaatcgat agactacgtt tagagcatga aaatagcttg caagaggcgc taacaaaagt
    20161 cgaacgagaa agagatgcaa tacaaaatca gttgcacatt caagaaaaag aaaaagattt
    20221 agctttagct tcagtaaaaa gtgattatga agtacaacta aaggcagcca atgaacaagt
    20281 agaattctat aaaaacttca aagctcaaca gtctactaaa gcagtaggag aaagtttaga
    20341 acattatgct gaaacagaat ttaataaagt gcgacatttg gcctttccta atgcttattt
    20401 tgagaaggac aatacattat caagtcgtgg ctcaaagggg gactttatct atcgagaaaa
    20461 ggatgaaaat gaccttgagt ttttaagtat catgtttgaa atgaaaaatg agtctgatga
    20521 tactatcaag aagcataaaa atgaagattt tttcaaagaa ttagataaag atcgtcgtga
    20581 aaaatcttgc gaatacgcag ttttagtaac tatgcttgaa gcagacaatg actattataa
    20641 tactggaatt gttgatgtta gtcacaaata ccctaaaatg tacgttatac gtccacaatt
    20701 ttttatccaa ttaattggta ttctaagaaa tgcagcactc aataccttaa aatataaaca
    20761 agagcttgct ttgatgaaag aacaaaatat tgacatcaca cattttgaag aagatttaga
    20821 tattttcaaa aatgcatttg ctaaaaatta taattctgca agcaaaaatt tccagaaagc
    20881 aatcgatgaa atagataaat ctattaaacg tatggaagct gttaaggctg ctttaacaac
    20941 gtctgaaaat caactacgtc ttgcaaataa taaattagac gatgtttctg tcaagaaatt
    21001 aacaagaaaa aatccaacaa tgaaagcaaa attcgatgct ctaaaagact aagaggttat
    21061 gattatgaag attagaactg ctacactaga cgatagtgaa aaattagtcc ctttgtatca
    21121 agaattagga tacgcaatat cattatcaga gattcaatct attttaaagg ttattcttac
    21181 tcattcagat tatggctttt tgatagctga ggataatggc aaactactcg ccttcgttgg
    21241 atatcataaa ctttactttt ttgaaaaaag tgggacttac tatcgtattt tagcactagt
    21301 agttaatgaa aagcaccgtc gtaaaggcat cgctagtcaa cttattaatc acgttaaaca
    21361 gctagctaaa acagatggaa gtgaagtact ggctttaaat agcagtttaa aagaatatcg
    21421 tcaggaagct tatcattttt atgaaaacct agggttcaag aaggttagta caggcttttc
    21481 atactattta gattaggtat aaatccttat agcactatac caaaagagcg taagttttta
    21541 cgctcctttc tttgtgttac cgcttattta ctgatataat ataaaacatg attacaggaa
    21601 taattaattt aaagaaagaa gctggaatga catcacacga tgctgtattc aagcttcgaa
    21661 agatattaca tacaaaaaaa ataggacacg gcggaactct tgatccagat gttgttgggg
    21721 ttttaccaat tgcggttgga aaagccactc gtgttattga atatatgaca gaaagtggca
    21781 aaatttatga aggagaaatc actttaggat atgccacaag tacagaagat tctagtggag
    21841 aggtcatttc aagaactcca ctaactcaat ccgatcttag cgaggatgtt gtggatcatg
    21901 cgatgaaaag ttttaccggt ccaattacac aagttcctcc tatgtattca gcagttaaag
    21961 taaatggtaa aaaactctat gaatatgctc gttcaggaga agaagtagag cgacctaaac
    22021 gacaaataac aatttctgaa tttagacgca caagcccatt gtacttcgaa aaaggcatct
    22081 gccgttttag tttctatgtc tcatgcagta aaggtaccta tgttaggacg ttagctgttg
    22141 atctaggaat aaaactagga tatgctagcc atatgtcttt cctaaaaaga acctcatctg
    22201 caggattatc tattacacaa tcattaacgc ttgaagaaat taatgaaaaa tataagcaag
    22261 aagatttttc atttttactt ccaattgaat atggtgtttt agatctccca aaagttaacc
    22321 taactgagga agataaagtt gaaatctcat acggacgtcg tatcttatta gaaaatgaag
    22381 cagatacctt agcagctttt tatgaaaata gagttatcgc tatactagaa aaacgtggca
    22441 atgaatttaa accacacaaa gtattgttat aaagaaagtc aatcatgaaa atccattaca
    22501 ttaatgatta taaagatatt caagctaaag aagattgcgt cttagttttg ggctattttg
    22561 atggcttaca tctgggacat aaagccctct ttgataaagc taaaaaaatc gcgaccgaaa
    22621 aaaatttgaa aattgtcgtc ttaacgttta atgagacacc tcgtctaacc ttcgctcgct
    22681 tccaacctga attattgcta catcttacct ctccagaaaa acgttctgaa aaatttcaag
    22741 agtatggcgt cgatgagtta tatttaatga attttaccag tcatttttca aaagtatctt
    22801 ctgacctttt tattaagaaa tatatttatg gtctcagagc aaaagctgct gttgtcggtt
    22861 ttgattataa gttcggtcat aatcgtacca gtggtgatta tctagcgcgt aatttcaagg
    22921 gaccagtcta tattattgat gaaatttctg aaggtggtga aaaaatctct tctacacgta
    22981 ttcgtcaatt gattacagag ggtaatgttg agaaagctaa ccaacttttg ggttatgagt
    23041 tttcaacctg tggcatggta gtacatggag atgctagagg acgaactata gggttcccaa
    23101 ctgctaatct agctcctatt aatcgcactt acttacctgc agacggtgtt tatatttcca
    23161 atgttctgat taatggaaaa tattatagag caatgacgag cattggaaaa aatatcactt
    23221 ttggtggtac agaacttcgt ttagaagcta atatctttga ttttgatggc gacatttatg
    23281 gagaaactat tgaaattttc tggttaaaaa gaatcagaga aatggtaaag tttaatggta
    23341 ttgacgatct tgtaaaacaa cttaaaaaag ataaagaaat tgctttaaat tggaaaaaag
    23401 atagtcaaac tctttaattt tttgtataat agtctctagt aaatacaaga ggcggttata
    23461 tgattacctt atttttatca cccagctgta ctagttgtcg aaaagcgcgt gcttggctaa
    23521 gtaagcatga ggttgctttt gaagagcata acattattac aagtccactc aataaagaag
    23581 aactattaca aattctatcg tttacagaaa atggtacaga agacattatt tccacacgtt
    23641 caaaagtctt tcaaaaactt gctattgatg tagacgaatt atcaacgtct tcattaatgg
    23701 aattaatttc agaaaatcca agtcttttac gacgcccaat tattttagac aaaaaacgta
    23761 tgcagattgg ttttaatgag gatgaaattc gtgccttctt accgcgtgac tatcgtaaac
    23821 aagaactgaa acaggccact attcgtgctg aaattgaggg aaaacatgat tagtagtaat
    23881 tatagttatc ctcttgatcc ttcatggaat acggaagata ttactaaggt acttcgcttt
    23941 ttaaatcaag ttgaacacgc ctatgaaaat agtgttaaag ttgatgattt gctagatagc
    24001 tataaagaat ttaaaaaatt tgttaaatcc aaggcgcagg aaaaacaaat tgaccgtgaa
    24061 ttccagagaa ctagcggtta ttccacttat caagctgtta aagcagcaca acaacaagcg
    24121 aaaggattta tttcacttgg acgctaaatt tgatttcgct aaacaattag tgtataaagc
    24181 tggtcaattc attaaatctg agatgcaaaa tacttttgat gttgaggaaa agtctcgatt
    24241 tgatgattta gtgacaagtc ttgataaaaa aacacaaaaa ttattaattc aagaaattat
    24301 tcaacattac ccagatgata atatacttgc cgaagaagat gatgtgcgct cacccattgc
    24361 acaaggtaat gtttgggttt tagaccctat tgatggaact gttaatttta ttgttcaaaa
    24421 agataacttt gctgttatgt tagcttatta cgaagaaggc gttggacaat ttggtatcat
    24481 ctacgacgtt atggctgata ttctttacag cgggggagga cattttgatg tctatgctaa
    24541 cgataaaaaa attgttcctt ttcaagagtg tcctttagaa cgttgcttac ttggagtaaa
    24601 ttctgctatg tatgcagaaa atgattgcgg tattgcccat ttagcttctg aaaccttagg
    24661 tgttcgtatc tatggtggtg ctggaattag catggcaaaa gtcatgcagg gcaaattatt
    24721 agcttatttt tcgtatattc aaccttggga ttatgctgca gcaaaaatca tgggggagac
    24781 attaggattt accttattaa cgcttgacgg ggaagaaccc aactattcaa caagacaaaa
    24841 agtaatgttc ctaccaaagt cgaaattaaa tcttatccaa tcctacctta ccaaaaaata
    24901 aacttaaaac cttctaatta aatagaaggt ttttttgcac gcccgtgatt atgtagtata
    24961 atagaaacat gaaattaccg aatgaattta ttgaaaaata tcagactata cttaaagatg
    25021 aagcagaagc tttttttgac agttttgaac aaaaacccat atcggcatat cgcaccaatc
    25081 ctctcaaaga aaagcaactt gattttccaa atgccatccc aagtacccct tgggggcatt
    25141 atgggaaaat atcaggaaaa tcaatagaac acacaactgg ccttgtttat tctcaagaac
    25201 ctgctgctca gatagtggct cagattgctg aaccacaaga aggaatgaaa gtgctagatt
    25261 tagcagctgc acctggtgga aaaacaacac accttctatc atatttaaac aatactggtc
    25321 tattagttag taatgaaatt tcaaataaac gtagtaaaat tttagtcgaa aatgtcgaac
    25381 gttttggtgc tagaaatgtt atcgtgacta atgaaagctc ccaacgacta gccaaatgtt
    25441 ttaattcttt ttttgatctt attgtttttg acggtccgtg ttctggtgag ggaatgttcc
    25501 gcaaagatcc tcaagcaata caatattggc acaaagatta ccccactgaa tgtgcacagt
    25561 tacaaagaga tattttaaaa gaagcaatca aaatgctagc tcatggtggt atacttgtat
    25621 actctacttg tacatggtca ccagaagaga acgaagaagt ggttaattgg ctccttcaag
    25681 agtatgatta cttagaactt gttgatattc ctaaactaaa tgggatggtt gaagggatta
    25741 atgtaccaca agttgcaagg atgtaccctc atcattttca aggtgaagga caattcgttg
    25801 caaaactgag agatactaga tctaaggaag cacagaaaat taagccaaaa gcacagaaaa
    25861 taaataaaat gcagttacaa ttgtggcaac aatttgcaca agaccattta aagatagact
    25921 tgaacggtgt tttagatgtt ttcggcgacc aactctatct tctacctaat ggtctccctg
    25981 atttgtctaa attaaaaata gcacgcaatg gtctccatct aggcactttc aagaagaatc
    26041 gttttgagcc atcgtttgct ttaggaatgg cacttagcga acatgaccta gtacagtcta
    26101 ttgaaattga tatagaacag tttgaggtgt acgtatctgg aaatgtagtc aaactagaca
    26161 agactgttcc aaacggttgg tatcaaattc ttgtaaaagg caatggatta ggttttgcaa
    26221 aagtgacaaa taatactctt aaaaattatt accctaaagg actacgattt cagacataac
    26281 tgttattgaa tacttttaat aaaaaaggaa aaagaaatac ttttcaaacc ctagaaactt
    26341 atggtactat gggacagtga tgtgtgtttt tactatttgt aaatcattaa acactcaatg
    26401 attttagtaa tatatatcaa ctactaaata tcaaaattta ttcaaaagaa taggaaaatg
    26461 aaaatgaaaa aaaacatttt tttgttaacc ttgctagcca ccgtcttatt gacgctgtct
    26521 ggttgtgcta attggattga taaaggtcaa tcaatcacca gcgttggttc aaccgcctta
    26581 cagccacttg tagaagctgc agcagatgag tttggtaaga caaacctcgg taagactatc
    26641 aatgtccaag gaggaggctc aggaacaggc ttatcacaag tacagtctgg agctgttcaa
    26701 attggtaata gcgacttatt tgcagaagaa aaagatggta ttgatgctaa gaaattgaaa
    26761 gaccatcaag tcgcagtagc tggattagct gttattgtta ataaaaaagt taatgttaaa
    26821 aatctaacca cccaccaact tcgtgatatt tttgcaggaa aaatcaagaa ttggaaagaa
    26881 gtgggaggac aagatttaga tatttccatc atcaatcgag cagcaagttc tggatcccgt
    26941 gcaaccttcg ataatactat tatgggaaat gttgctccta ttcaaagcca agaacaagat
    27001 tcaaatggta tggtgaaatc aattgtttct caaacacctg gtgcgatttc atacctcgct
    27061 tttgcatacg ttgataaatc tgtcggaaca ttaaaactaa atggttttgc gccaacagct
    27121 aaaaatgtta ctaccgacaa ctggaaactt tggtcatatg aacatatgta tacaaaaggt
    27181 aatgaaacag gtctaacaaa agaattctta gactacatga aaagtgataa agttcaaagt
    27241 agtatcgtac aacatatggg atatatctct attaacgaca tgaaagttgt taaagatgct
    27301 gaaggtaaag taaccccaaa ataaaactta atcacatcta gataaagcat cttgatccta
    27361 ggaggaatta tgaaaaatca agaattagct aaaaaattaa catctccttc aaaaaactct
    27421 cgattagaaa aatttggtaa aggaataact tttctttcgc ttgccctaat cgtcttcatt
    27481 gttgcgatga ttttgatttt tgttgctcaa aaaggactat ctacattttt tgttgatggc
    27541 gttaaattaa cagacttctt atttaataca aaatgggaac caagtgctaa gtcattcggt
    27601 gctcttccaa tgatagctgg atcatttatt gttactattc tatcagctat cattgctaca
    27661 ccatttgcta tcggtgctgc cgtatttatg actgaaatct cacctaaata tggttctaaa
    27721 attttacaac ctgcagttga attactagtt gggattcctt ctgttgttta cggttttatt
    27781 ggtttacaga ttattgttcc cttcgttcgc tctatttttg ggggaacagg ttttggcatt
    27841 ttatctgggg tctgtgtcct cttcgtcatg attctaccaa ctgttacttt catgacagta
    27901 gatagcctta gagctgtgcc acgtcactat aaagaagcaa gtttagccat gggggcaact
    27961 cgttggcaaa caatttggcg tgttatatta aacgctgcac gtccaggaat tttcactgca
    28021 attgtttttg gtatggcacg tgcatttggt gaagctctag caattcagat ggttgtcgga
    28081 aactctgcaa ttttaccaac atcattgaca acaccagcag caacattaac gtctgttcta
    28141 acaatgggta ttggtaatac tgtaatggga actgtacaaa acaatgtcct ctggtcgcta
    28201 gccttagtac ttttaattat gagtttagca tttaatacag ttattaagtt aattacaagg
    28261 gaaggaaaga aaaactatga acgctaaaaa ggctgacaaa ttagcaacta caatactcta
    28321 tagtattgca gctatcatcg ttacaatcct cgcctctctt ttaattttta ttcttgttcg
    28381 cggattgcca cacgtatctt ggtcattttt aactgggaaa tcatcatcat atgaagcagg
    28441 aggcggtatt ggaatacaat tatacaattc ctttttccta cttattgtta ctctgattat
    28501 ttctattcct ctatctttag gagctggaat atacctctca gaatatgcta aaaaaggacg
    28561 tttaacaaac tttgttagaa catgtatcga aattttgtca tctcttcctt ctgttgtagt
    28621 tggtctgttt ggttacctaa tcttcgttgt tcaatttcaa tatggcttct caattatctc
    28681 aggtgcctta gcacttactg tttttaactt accccaaatg acacgtagtg ttgaagatag
    28741 ccttcaaaat gtacaccaca cacagcgtga agcaggattg gccctaggta tttctcgttg
    28801 ggaaacggtc atttacgtcg ttgtccctga agctctgcca agtattgtaa ctggtgttgt
    28861 gctagcttca ggacgtattt tcggtgaggc agcagcactt atttatacag ctggtcaatc
    28921 tgctccagca cttgattggt caaattggaa cgtccttagt gttacaagtc caatctcaat
    28981 tttccgtcaa gcagaaactt tagctgttca tatttggaaa gttaatagcg aagggacaat
    29041 ccctgatgca acacaagtat ctgccggtag cgccgcagtc ttattggttg ttattcttat
    29101 tttcaacctt tcagctcgct cgatcggtaa gaaacttcat tcgaaattaa cctcgtctaa
    29161 ttaaaaggag tagtaatggc agaatataat tgggacgaac gccatatcat taccttccct
    29221 gaagaaaatt cagctctcac cacaaaagat ttacacgttt actacggtga gaaagaagct
    29281 atcaagggaa ttgatatgca atttgaaaaa aataaaatta cagccttaat cggcccatct
    29341 ggatgtggaa aatcaacata tcttcgtagc ttaaatcgta tgaacgatac gattgatatt
    29401 gcacgcgtga ctggacaaat catgtatgaa ggaattgatg ttaacgctca agatatcaat
    29461 gtttacgaga tgcgaaaaca tatcggaatg gttttccaac gtccaaaccc gtttgcaaaa
    29521 tcaatttata aaaacattac ctttgcatat gagcgtgctg gtgtaaaaga taaaaaattc
    29581 ttagatgaag ttgtcgaaac ctcactaaaa caagctgctc tatgggatca agtaaaagat
    29641 gaccttcata aatcagcatt cactttatct ggcggtcaac agcagcgatt atgtattgca
    29701 cgtgctatcg cagttaaacc agaaattctc ttaatggatg aacctgcttc agcactcgat
    29761 ccgattgcaa ctatgcaact agaagagaca atgtttgaac tcaagaaaaa ttacactata
    29821 attatcgtaa cacataatat gcaacaagca gcacgtgcta gtgattatac tgcatttttc
    29881 tatcttggag acttaatcga atacgataaa acgaataaca ttttccaaaa tgctaaatgt
    29941 caatctacta gcgattatgt ttctggtcga tttggatagt gaggtaaaag atgacacaac
    30001 ctattttaca agtcagtgat ttgtctgttt actataataa gaaaaaagct ttaaaagaag
    30061 tctcaatgga cttctatcct aatgaaatta cagccttaat tggtccgtct ggttctggaa
    30121 agtcaacact cttacgagct attaaccgta tgggagactt aaatcctgaa gtcactctaa
    30181 caggagcagt gatgtataac ggacataatg tttatagccc gcgcactgat accgtagaac
    30241 ttcgtaaaga aattgggatg gtttttcagc agcctaatcc atttcctatg tcagtatttg
    30301 aaaatgttgt ttatggtctt agactcaaag gaataaaaga caaagctacg ttagatgaag
    30361 ccgtagagac ttcattgaag ggcgcgtcta tctgggacga ggtcaaagat agattacacg
    30421 actctgctct tggtttatct ggtggtcaac aacaacgtgt ttgtattgcc agaacccttg
    30481 ctaccaagcc taaaattatc cttctagatg agccaacctc tgcacttgac ccaatttctg
    30541 ctggtaaaat tgaagagact ttacatggtc taaaagatca atataccatg ttacttgtta
    30601 cacgttctat gcagcaagcc tcacgtattt ctgatcgtac aggtttcttc ttagacggta
    30661 acttaattga atatggcaat acaaaagaaa tgtttatgaa tcctaaacat aaagaaactg
    30721 aagattacat tacaggtaaa ttcggttaat accatataat aagatcagaa agagaataca
    30781 atatgttaag atcaaaattt gatgaagaat tagataaact gcataaccaa ttctatgcaa
    30841 tgggaattga agctatcgga caaatcaaaa aaacagttcg tgcttttgtc agtcatgatc
    30901 gagagttagc taaagaagtg atagaagatg atgtaacgct taacaacttt gaaacgaagt
    30961 tagagaaaaa atcattggaa attattgcac tccagcagcc tgtatcacaa gacttacgca
    31021 ccgttattac cgttttaaaa gctactagtg atgttgagcg tatgggagac catgcagcag
    31081 cagttgcaaa agcaaccatc cgtatgaagg gcgaggaaag aatcccagca gtagaattag
    31141 aaatcaataa tatgggaaaa gcagttaaaa acatgcttga agaagcctta acagcttata
    31201 tcaatggtga tgatgaaaaa gcttacgaag tagcagcaat ggatgaaata gttgatgatt
    31261 acttccgtga tatccaaaaa atggtagttg aaacaattca aaagcatcct gacgtagcat
    31321 ttgccgccaa agaatacttc caagtattga tgcacttgga gcgaattgga gattatggca
    31381 aaaatatctg tgaatggatt gtttatttaa aaactggaaa aattatcgag ttatgatata
    31441 attattgctt gtagagtggt aattgtaagt atagttcgtg cgatagaaaa gctgtaacta
    31501 tcttgttaca gcttttctat cttttctagc ccatacagga ctagtaatgt ttcaaatgtt
    31561 tctgaattag ttaggagtta tatgaaaact gtagaacatt ttgttaccaa atttgtccct
    31621 gaaaactata accttttctt ggatattaat cgtcaaacta aaaccttttc aggtaacgtt
    31681 gctgtgtctg gcgaagcctt agataacaat atctcttttc accaaaaagg gttaaccatt
    31741 aaatctgtct tattagataa tcaaccatta gatttccaac tagatgaaga caatgaagct
    31801 atgcatattc aattgcatga aactggtagc atggtattag tctttgaatt cagcggtcac
    31861 attaccgata atatgacagg tatgtatcca tcttactata cagttaatgg catcaaaaaa
    31921 gaggttatct ccacacaatt tgaaagtcat tttgctcgtg aagtattccc aagtattgac
    31981 gaaccagaag caaaagcaac ctttgatttg agcctaaaat tcgatcaaaa agaaggagag
    32041 attgctcttt caaatatgcc ggaaatcaat gctgaacaac gtcaagaaac aggtttatgg
    32101 acatttgaca ccacacctaa aatgtcctca tatttattag cttttgcctt gggggaattg
    32161 catggtaaaa caacacatac caaaaacggc actcttgttg gctcatatgc cacaaaagcc
    32221 catcagttaa atgaattaga tttttcttta gatatcgttg tgcgtgttat tgagttttat
    32281 gaggactact ttggtgtacg ttatcctatt cctcagtcac tccacgttgc tctacctgat
    32341 ttttctgctg gagctatgga aaattgggga ttagtaacat atcgtgaagt ctatctttta
    32401 gtagatgaga actctagtgt ttccagccgt cagcaagtag cattagttgt agcacatgaa
    32461 atagctcacc agtggtttgg taacctcgtt actatgaaat ggtgggatga tttatggtta
    32521 aatgaatcat ttgctaatat gatggaatat gtatcaattg attatattga accaaaactt
    32581 aatatatttg aagactttca aacaggtggt ctaccattag ctcttaagcg tgatgccaca
    32641 gatggcgtcc aatctgttca cgttgaggtc aatcatcctg atgaaattaa cacacttttt
    32701 gatccagcta tcgtttatgc aaaaggtagc cgcctcatgc acatgttacg tcgctggtta
    32761 ggcgatacag attttgccgc aggactaaaa atttattttg aaaaacatca atatcaaaat
    32821 actattggtc gtgacctttg gaatgcactt tctcaaacct caggaaaaga tgttgcagct
    32881 ttcatggatt cttggttaga gcaaccagga taccctgtga tggcagccaa aatagaagaa
    32941 gatgagctca ttttaactca aaaacaattc tttattggtg aacacgaaga taagtctcgc
    33001 ttgtggcaaa ttcctcttaa tagtaattgg gaaggaatcc cagagatatt aaccgaggaa
    33061 acggtagtaa tccccaattt tagtcagtta gctgaaaaga acaaagaaaa tggtgcactg
    33121 cgtttcaata ctgaaaatac tgcacattac attactaact atcaaggtca actcttagag
    33181 catatcattt ctgatcttcc tcttatggat aacatttcta aactacaaat tgtacaagaa
    33241 cgtcatttac ttgctgaaag tggtatgatt tcatatagta gtttaatacc tttagtctct
    33301 cttttgagcc aagagacatc atatcttgtt aactctgcta ttaagagtgt tattgatggt
    33361 ttatccttat tcgtccagga agatagccaa gatgaattcg actttaaaga atttgttaat
    33421 aaattatcag catttaattt taatagactt ggctttgaaa aaagagaagg agaaggagat
    33481 gatagcgaaa tggtccgtca tctatcccta agccttgctc tctactcaga taatgaacat
    33541 gctattgaag aagctcacca tatttttaaa gcccacgaaa ataatatcgc tgctatacca
    33601 gctgcaattc gcctgttagt tcttaccaat gaaatgaagc atttcgaatc caaagaactt
    33661 agccacttgt tattagagac ttatagtact actaccgatg ggaatttcaa acgacaacta
    33721 gcgagtgctt tatctcatac tacagatagc aagacactca agaaattatt gtcagattgg
    33781 aaaaataaag atattgtgaa accacaagac ttagctatga gttggtatgc tactttcctt
    33841 aaaaattctt ttacacaaga aagtgtctgg gagtgggccc aagaaaactg ggaatggatc
    33901 aaagcaactc taggtggtga tatgagtttt gataaatttg tgatttatcc ctcaagtagt
    33961 ttcaaaacag aagaacgcct tgaacaatat aaaaactttt tcgagcctca attaagcgat
    34021 atggcgatta gccgtaatat ttcaatggga attaaagaaa tttctgctcg tgtcctttta
    34081 atcacgaagc aaaaagaaga agttatcaat actatcaaaa aatatatcta ataaactcaa
    34141 atagagattg atggattatt caatctctat ttctttgtct gtgtcatgaa attagttgac
    34201 ataaaacata ctttatttag gatttattaa gcaaagtttg gtattctaat attaatagtt
    34261 cagataaaat atgtacatcg aaaggatata ctatgataaa aattttatta attgaagatg
    34321 acttgagtct ttccaattcc gtttttgatt tccttgatga ctttgccgat gtcatgcaaa
    34381 tttttgatgg agaagagggt ctttacgaag ccgaaagtgg agtctatgat ctgattcttc
    34441 tagatctcat gttacctgaa aaaaacggct tccaagtgtt gaaagagcta cgtgaaaaag
    34501 gtattacaac ccctgtatta ataatgaccg ctaaagaaag cattgatgat aagggacaag
    34561 gtttcgactt aggtgctgat gattatctaa caaaaccttt ttacctagaa gaactaaaaa
    34621 tgcgaattca ggctctttta aagcgttctg gaaaatttaa tgataacagc ctaatttatg
    34681 gtgatattcg cgtagacatg tctactaact caacctttgt taatcaaaca gaagttgagc
    34741 tcttaggtaa agagtttgat cttttagtct atttccttca aaatcaaaat gttatcttac
    34801 cgaaatctca aatctttgat cgtatttggg gatttgatag tgatacaacg atttcagttg
    34861 tagaagtcta tgtctctaag gttcggaaaa aattaaaagg aacacttttt tcagaaaacc
    34921 ttcaaacttt gcgtagtgta ggatatattt taaagcatgt cgaaaccgat taagataagt
    34981 aaattcaaaa aaaatgtctc agatagtcat tttatccatt ttttcacagt ttttagtggt
    35041 atttttctgg ttatgactgt tatcatcctc caagtcatgc gctatggtgt ttattcttct
    35101 gttgatagta gtttaaagta tatcagtacc catcctaaaa attacatcaa tatggttatg
    35161 tcacgtactg ctgcctacct agataactcc aatatcgcaa gtgtcaagtt aaaacccggc
    35221 ggtcagacag tagctaatac tgatattctt ctttttacat cagaagaaga agtcattaac
    35281 tattttgacg cattttcaaa ctatcagttt ttaaaaccta ataaaaaaaa tctaggtggc
    35341 attagtgaat taactttaac caacatcttt ggtcaagatg agacatatca tgctgtcacc
    35401 gtaaaagtta ataaccctgc ttatcctaat gtgacttaca tgacagctat tgttaatatt
    35461 gatcaattag taaatgctaa agaacgctat gaaaaaatta ttatatttgt catgactact
    35521 ttttggatta tttcaattgg agcaagtatc tacttagcta aatgggcaca aaaaccgatt
    35581 atagaaaact acgaaagaca gaaggctttt gtcgaaaatg ctagccacga gctgcgtact
    35641 cccctagctg tcttacaaaa tcgcctagaa acgctattca gaaaaccgaa tgccaccatt
    35701 ttagaaaata gtgaaaatat cgcttcaagt ctagatgaag tacgaaatat gcgtatttta
    35761 accactaatc ttttaaactt agcaagacgt gatgatggaa tcaaaccaga attagctgtt
    35821 attaagccta cactttttga tagtatcttt gagaattatg atctaatcgc ccaagagaat
    35881 ggtaaaaatt ttactggaca taatatgatc caagatagtt tcaaaacaga taagacactg
    35941 ctaaaacaac tcatgactat tttatttgat aatgctataa aatatactga caatgatggt
    36001 agcattgact ttaccatatc tgaaactgat aagtatctct ttttagaaat cgctgataat
    36061 ggaccaggaa tttcagagga ggataaagtc agaatttttg atcgttttta ccgtgttgac
    36121 aaagcaagaa cacgacaaca aggagggttt ggcctcggcc tttctttagc tcaacaaatc
    36181 gttaactcac tgcgaggtaa tattacagta attgataata aacctagagg cagtattttt
    36241 aagataaaac taccaaaagt aaaaggttag gccatgttag tcctaacctt ttaaatttta
    36301 aagtggtctt gcatacttga tatagcaagc tgataggaaa tttggtataa taaaagctat
    36361 ggaaattgaa aaaacaaatc gtatgaatgc cctttttgag ttttatgcgg cattattaac
    36421 agataaacaa atgaattata tcgagttata ctacgccgat gactacagcc ttgctgaaat
    36481 agctgaagaa tccggtgtta gccgacaagc tgtctatgat aatatcaaac gaactgaaaa
    36541 aattcttgag gcgtacgaaa tgaaactaca tatgtattca gattatattg tccgtagtca
    36601 gatttttgat gacattctag aaaaatatac cgatgatgcc tttttacaag aaaaaatttc
    36661 cattctaagt agcattgata atagagatta aatgaggaga aataatttat ggctttcgaa
    36721 agtttaacag agcgcctgca aggagttttt aaaaatattc gtggtaagaa aaagttatct
    36781 gaaaaagatg tgcaagaagt caccaaggag attcgtctag ctcttttaga agctgacgtt
    36841 gctcttccag ttgttaaaac ctttattaaa catgttcgtg aacgtgctgt tggacatgaa
    36901 atcattgata cactagatcc tacgcaacaa attgttaaga ttgtcaatga agagctaaca
    36961 gatttattag gggctgagac ctctgaaatt gaaaaatccc ctaaaattcc aaccatcatt
    37021 atgatggttg gtctacaagg tgctggtaaa acaacttttg ccggtaagtt agcaaacaaa
    37081 ctaatcaaag aagacaatgc tcgtcctatg atgatagcag ctgatattta tcgaccagct
    37141 gctattgatc agttaaaaac ccttggtagc cagattaatg ttcctgtctt tgatatggga
    37201 actaaccatt cagcagttga gattgtcact aaaggtttag aacaggctcg tgaaaatcgt
    37261 aatgactatg ttcttattga tacagccgga cgcctacaaa ttgatgcaac ccttatgcag
    37321 gaattgcatg atgtcaaagc catagctcaa cctaatgaaa tactccttgt cgttgacagc
    37381 atgattggac aggaagctgc aaatgtcgct gaagaattta accgtcagct cagcattagt
    37441 ggtgttgttc tgacaaagat tgacggtgat acacgcggtg gtgctgccct ttcagtacgc
    37501 gaaattactg gaaaaccaat taaatttaca gggacagggg aaaaaatcac tgatattgaa
    37561 actttccatc ccgatcgtat ggcaagccgt atattgggga tgggagacct gctaacactt
    37621 attgagcgcg ctagtcaaga atacgacgaa aaacgttcaa tggaattagc ggagaaaatg
    37681 cgtgaaaaca cctttgactt caacgatttt attgatcaat tagatcaagt tcaaaatatg
    37741 gggccaatgg aagatctcct taaaatgctt ccaggaatgg ctaacaatcc agcaatgaaa
    37801 aacttcaaag tggatgaaaa cgaaattgct cgcaaaagag ccattgtgtc atcaatgact
    37861 ccagaagaga gagaaaatcc aaatctcctc aatccaagtc gtagacgtcg tattgctgct
    37921 ggatcaggta atactttcgt tgatgttaat aaattcatta aagattttaa ccaggcaaaa
    37981 caaatgatgc aaggcgtgat gtccggtgat atgaataaaa tgatgaaaaa aatgggaatt
    38041 gatcctaata acctccctaa agatatgcct ggaatggacg gaatggatat gtctaattta
    38101 gaaggtatga tggggcaaaa tggcatgcct gacttatcta gtttaggtgg cgatatggac
    38161 ttcagccaaa tgtttggtgg tggcttaaaa ggaaaagtag gagcctttgc agctaaacaa
    38221 tcaatgaaac gtatggctaa taaaatgaag aaagctaaga agaaacgcaa ataaaaatgt
    38281 ctatttaaat agacattttt atttttgtct gctataatag acaaaaaggt ggtgatattt
    38341 atgtatttag cattaatcgg tgatatcatt aattcaaaac agatacttga acgtgaaact
    38401 ttccaacagt cttttcagca actaatgacc gaactatctg atgtatatgg tgaagagctg
    38461 atttctccat tcactattac agctggtgat gaatttcaag ctttattgaa acaatcaaaa
    38521 aaggtatttc aaattattga ccatattcaa ctagctctaa aacctgttaa tgtaaggttc
    38581 ggcctcggta caggaaacat tataacatcc atcaattcaa atgaaagtat cggtgctgat
    38641 ggtcctgcct actggcatgc tcgctcagct attaatcata tacatgataa aaatgattat
    38701 ggaacagttc aagtagctat ttgccttgat gatgaagacc aaaaccttga attaacacta
    38761 aatagtctca tttcagctgg tgattttatc aagtcaaaat ggactacaaa ccattttcaa
    38821 atgcttgagc acttaatact tcaagataat tatcaagaac aatttcaaca tcaaaagtta
    38881 gcccaactgg aaaatattga acctagtgcg ctgactaaac gccttaaagc aagcggtctg
    38941 aagatttact taagaacgag aacacaggca gccgatctat tagttaaaag ttgcactcaa
    39001 actaaagggg gaagctatga tttctgattt tttaagggat aatccaatcc taactttatt
    39061 attttgcgca cattttttag ctgactttca gtggcaaagt caatccttag cagatagtaa
    39121 atcacatagt tggaggggac tctggagaca tctcttgatt gttttcctcc ccctagcagc
    39181 tttaatgata ttgataccag aaactacttt gttaaattta agcatatggg gaagtcatat
    39241 tgttattgat agtataaaga aattgagtta cccttgggta gaggaagggc atttccaaaa
    39301 agcagctttt attatagacc aactagccca ctatacttgc attatcgttt tttaccatgc
    39361 gcttcctaca tatttaccgc ctaatcattg gcttttgcct atcaagcact ttattgtcat
    39421 agctctggtt tttatcatta tcaccaaacc cattaatatt gtgtttaaaa ttttctttaa
    39481 taaatttcaa gctaaagaat tatcatcatt attaacacaa gaaaaaacaa aaataatgaa
    39541 agaaaaatct gaagatcatg aagaaacaat tgaaggagca ggagctatga ttggtaattt
    39601 agagcgtcta attatggcta tattactaat ctcaggacag tatgctgcta taggtcttgt
    39661 ttttactgca aaatccattg cacgttacga taaaatttct aaaagtcagg tttttgccga
    39721 atattattta attggatcat tgttctcaat tataagtgtt ttgataactc attgggtact
    39781 tttggtatag aaaagcgcta gaaatatcct aacgcttttc ttttattctg tcttataagc
    39841 ataagtaaaa tcaacattca aaccagttga atttcttacc aaccccttta atttagaatt
    39901 ttggagtcct tttttcccta ggtaataaac tgggttataa tatgattgat caaataaagc
    39961 tttttcagca gttttgtaat cattagcggc atctcctggt ttcaaggcgt cagtagtaat
    40021 tgccttttga taggcattgt catactcttt agaagaaaat ttaccataat tatacgcaga
    40081 attagtcgtg aataacccat agaaagttga tccttctgga taatctcctc cccatgagaa
    40141 gagcacgaca tcaaaatttt cattttttgc atcttcaagg cgttgcttga aagtaacaaa
    40201 tttttcttca acagttagac caggcaaagc actctcccat gtagatttca catagtcaac
    40261 agcattttta gctgctggag agtccgaatc agctgtgatt gttaacttga gagatgactg
    40321 accaacctca gcaagacctt ctttaaaaag tttttgagct ttacttgtat tatacgtata
    40381 acctggggca gtatacttgg aaagatcctc accattaggt aatttggcta attttttagg
    40441 gaccagagat tcagcaggag ttgaacctgt atcaactgcc gctttaacaa cacctttacg
    40501 atcagtcgct aaattcaaag cttgcctaat tttctgattt gtcaatgctt taaccgaacc
    40561 agtttggtta taaatagcag aagttcctga tatttcagca aaatcaatct gaccttgctt
    40621 gtacatttga actgcagtat ctggtttttt gatagtttga actatgacac tatttgtttt
    40681 aacatgttta gaatcccaat aatatttatt tttgactaat ttaaatttac catttgaacc
    40741 attccaatcc ttaacaaggt aaggacctga gtaaatttga ttttttgatg ttgtaccata
    40801 atctttacca actttttcaa catacgattg tttttgaggc ataaaattag agaaggctaa
    40861 ataatattta aattgagggc atggacttgt caatttaaag gtcacttgat tgcctttagc
    40921 tgttacacca agtttattta aatctttaat atctccagaa ttaatcttat cagcatttaa
    40981 aaggtgcgat tctgtcgcca agtaagcata ttcagatgca gttttaggat caactattct
    41041 ccgccatgtg taaacaaaat cttcagcgct tagtttgctt ccatctgacc attttaaatt
    41101 atcacgaagt gtagcagtat aagtaagacc atcagaagaa acggatactt ttttagctaa
    41161 atcgggtttt ggtttgccct ctttatcaat tctaagtaaa ttgctgcctg aattacctat
    41221 agctaagttt gaataagcat ctgtattctt tgaaatgtcc aatgtactga tttcagtagg
    41281 aatataccaa ttaataactt tattatctcc agagttcttt gactcattac cacaggcaac
    41341 cagggtaact gtggtggctg ttagagtcgc caaagatata cccaaaaact taagtttttt
    41401 tctattaaaa ctagacataa taccagtcct cctaaacctt tttttataag acatagtaga
    41461 tctaatattg agaaaactag tatattttaa agtagaattt aactttggtt attaacttgc
    41521 aaatataatt aaaaataatt gagtacactt tattaccttt tcagataccg ataaaatcaa
    41581 tgaaattatg aatcagtttt ctcaaaatac tctgtcttta ctataacaaa catgttaaac
    41641 gataataagt aatactgatc atcataaaga atggatattt gatgtactta ccgaatgaat
    41701 tcctgttttt aacttttaaa ctttccgaaa aactataatt ttcttgaaag ttatgataga
    41761 gtatgatata cttctattta tagatataat ggagatttta ttatgaaacg tgaattacta
    41821 ctcgaaaaaa tcgatgaact aaaagaaatt atgccttggt atgttctaga atattatcaa
    41881 tcaaagcttt ctgttcctta tagtttcaca acactctacg aatatttaaa agaatataga
    41941 cgttttcttg aatggttatt ggattctggt gttgctaatt gtcatcacat tgcagaaatt
    42001 gagctttcag ttttagaaaa ccttactaaa aaagatatgg aagcttttat actatattta
    42061 cgagaacgcc ctttattaaa tgctaacaca aggcaaaatg gggtgtctca aaccaccatt
    42121 aacagaactc tatctgcctt atctagtctt ttcaaatacc ttactgaaga ggttgagaat
    42181 gctgacgggg agccttactt ttatcgcaat gtcatgaaaa aagtctcaac caaaaagaaa
    42241 aaggaaactt tagcttctcg ggcagaaaat attaaacaaa agttattttt gggcaatgaa
    42301 acgatagaat tcttagagta tatagattgt gaatatcaaa acaaactttc caaacgtgct
    42361 ttagcttttt tcaacaaaaa taaagagaga gatttagcta ttattgcttt gctactagct
    42421 tcaggagtgc gtctatctga ggcagtaaac ttagatttaa aggatattaa ccttaatgta
    42481 atggttattg atgtcacacg aaaagggggg aaaagggact ctgtcaatgt cgctagcttt
    42541 gcgaagccct accttgctaa ttatttagac atccgtaaaa atagatacaa agcagaaaat
    42601 caggacattg ctctattttt atcagaatac cgtggggtgc caaatcgtat tgattcttca
    42661 agtgtagaga aaatggttgc taagtattcc caggatttta aggttcgtgt aacacctcat
    42721 aaactaaggc atactcttgc tactaggcta tatgatgcta caaaatctca agtattagtt
    42781 agccaccaac taggtcatgc ttccactcaa gttactgacc tatataccca tattgttaac
    42841 gatgaacaaa agaacgcttt agataaacta tagtaacata aataaagtta tgtaaatatt
    42901 ttattttaca taactttatt tatgtataga agcagcagtc ccataattaa ttttgctatc
    42961 caccgaaatg gacaaattca gactagataa gacttctttt gtcatatata accccatgcc
    43021 tgttgccttt tggtgctcat gaccgttgta acccgtaaag ccatcttcaa aaagtctagg
    43081 gatatcctct tcgagtatgc cgataccgta gtcttgtatt ctaatactct cttctcctat
    43141 gcttattatt atctttgact caggattaga atattttatg gcattatcta gcacctgtga
    43201 aagagcaaaa gttaaccact ttttgtctgt tttccagata ttatcgcctt caattatgat
    43261 agataagctt ttggatagac aaataacctt atacgatttt ataatttcta ctactacttc
    43321 tctaaggcta acagcttcaa aacgaaaatc gtcacgatat tgtctaaatt tcaaaaatgc
    43381 taacaacgtt tcaagataat gttgcaattt caataattgt tgttcaactt ccttaggatc
    43441 gagatgattt gtctgggcca ttaatgaaat agctgccaat ggaactttca tttggtgcga
    43501 ccacatcttt atcataagtg caacatgatt ggtttgttca attgtttcag aaactttttg
    43561 cgcttctcta gcttctaatt gtgtaataat attaaaataa gcgtaatcac tcggttgaag
    43621 ttcgaaagag gggtcctgac tactattaaa atatttgaga tgtaacattt ttttcctgta
    43681 acgactgtat tgccaaatac taattc
//
LOCUS       DATROY010000005        43000 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.005, whole genome shotgun sequence.
ACCESSION   DATROY010000005 DATROY010000000
VERSION     DATROY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43000
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..553
                     /gene="fetB"
                     /locus_tag="VBD11_000228"
     CDS             <1..553
                     /gene="fetB"
                     /locus_tag="VBD11_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6953208.1"
                     /translation="IINASYNAAKRGKGINKGFVISFIAIGSGTIITLSVLIFSGILK
                     FVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETKLALGADILPASIDIIR
                     DVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKYQMMVTFMLLATTSIAS
                     FVATYLAYKIFFNNRKQLVVTKD"
     gene            717..1535
                     /locus_tag="VBD11_000229"
     CDS             717..1535
                     /locus_tag="VBD11_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6953209.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(1572..2456)
                     /locus_tag="VBD11_000230"
     CDS             complement(1572..2456)
                     /locus_tag="VBD11_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6953210.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGVRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            2649..3707
                     /locus_tag="VBD11_000231"
     CDS             2649..3707
                     /locus_tag="VBD11_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6953211.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            3721..4713
                     /locus_tag="VBD11_000232"
     CDS             3721..4713
                     /locus_tag="VBD11_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6953212.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            4921..6471
                     /locus_tag="VBD11_000233"
     CDS             4921..6471
                     /locus_tag="VBD11_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO6953213.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            6538..7563
                     /locus_tag="VBD11_000234"
     CDS             6538..7563
                     /locus_tag="VBD11_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6953214.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            7580..9379
                     /gene="uidA"
                     /locus_tag="VBD11_000235"
     CDS             7580..9379
                     /gene="uidA"
                     /locus_tag="VBD11_000235"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6953215.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFVDFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            9408..10079
                     /locus_tag="VBD11_000236"
     CDS             9408..10079
                     /locus_tag="VBD11_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6953216.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            10196..10813
                     /gene="eda"
                     /locus_tag="VBD11_000237"
     CDS             10196..10813
                     /gene="eda"
                     /locus_tag="VBD11_000237"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6953217.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            10830..12230
                     /gene="uxaC"
                     /locus_tag="VBD11_000238"
     CDS             10830..12230
                     /gene="uxaC"
                     /locus_tag="VBD11_000238"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6953218.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            12248..13294
                     /gene="uxuA"
                     /locus_tag="VBD11_000239"
     CDS             12248..13294
                     /gene="uxuA"
                     /locus_tag="VBD11_000239"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6953219.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            13418..14257
                     /locus_tag="VBD11_000240"
     CDS             13418..14257
                     /locus_tag="VBD11_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6953220.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            14410..15222
                     /locus_tag="VBD11_000241"
     CDS             14410..15222
                     /locus_tag="VBD11_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6953221.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            15238..17028
                     /locus_tag="VBD11_000242"
     CDS             15238..17028
                     /locus_tag="VBD11_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6953222.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(17086..18171)
                     /locus_tag="VBD11_000243"
     CDS             complement(17086..18171)
                     /locus_tag="VBD11_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6953223.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            18381..19385
                     /gene="ccpA"
                     /locus_tag="VBD11_000244"
     CDS             18381..19385
                     /gene="ccpA"
                     /locus_tag="VBD11_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6953224.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            19517..20983
                     /locus_tag="VBD11_000245"
     CDS             19517..20983
                     /locus_tag="VBD11_000245"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6953225.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            21028..22026
                     /locus_tag="VBD11_000246"
     CDS             21028..22026
                     /locus_tag="VBD11_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6953226.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            22028..23362
                     /locus_tag="VBD11_000247"
     CDS             22028..23362
                     /locus_tag="VBD11_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6953227.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            23819..25762
                     /gene="thrS"
                     /locus_tag="VBD11_000248"
     CDS             23819..25762
                     /gene="thrS"
                     /locus_tag="VBD11_000248"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6953228.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            25983..26687
                     /locus_tag="VBD11_000249"
     CDS             25983..26687
                     /locus_tag="VBD11_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953229.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            26785..27804
                     /locus_tag="VBD11_000250"
     CDS             26785..27804
                     /locus_tag="VBD11_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6953230.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            27971..28537
                     /locus_tag="VBD11_000251"
     CDS             27971..28537
                     /locus_tag="VBD11_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6953231.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(28586..29236)
                     /locus_tag="VBD11_000252"
     CDS             complement(28586..29236)
                     /locus_tag="VBD11_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6953232.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(29248..29943)
                     /locus_tag="VBD11_000253"
     CDS             complement(29248..29943)
                     /locus_tag="VBD11_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6953233.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(29956..30756)
                     /locus_tag="VBD11_000254"
     CDS             complement(29956..30756)
                     /locus_tag="VBD11_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6953234.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(30768..31523)
                     /locus_tag="VBD11_000255"
     CDS             complement(30768..31523)
                     /locus_tag="VBD11_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6953235.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            31863..32573
                     /locus_tag="VBD11_000256"
     CDS             31863..32573
                     /locus_tag="VBD11_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953236.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            32566..33915
                     /gene="vicK"
                     /locus_tag="VBD11_000257"
     CDS             32566..33915
                     /gene="vicK"
                     /locus_tag="VBD11_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6953237.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            33919..34728
                     /locus_tag="VBD11_000258"
     CDS             33919..34728
                     /locus_tag="VBD11_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6953238.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            34731..35099
                     /locus_tag="VBD11_000259"
     CDS             34731..35099
                     /locus_tag="VBD11_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6953239.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            35274..35960
                     /locus_tag="VBD11_000260"
     CDS             35274..35960
                     /locus_tag="VBD11_000260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6953240.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            35968..39507
                     /gene="smc"
                     /locus_tag="VBD11_000261"
     CDS             35968..39507
                     /gene="smc"
                     /locus_tag="VBD11_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6953241.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            39598..40395
                     /locus_tag="VBD11_000262"
     CDS             39598..40395
                     /locus_tag="VBD11_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6953242.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            40395..41219
                     /locus_tag="VBD11_000263"
     CDS             40395..41219
                     /locus_tag="VBD11_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6953243.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            41219..42829
                     /gene="ftsY"
                     /locus_tag="VBD11_000264"
     CDS             41219..42829
                     /gene="ftsY"
                     /locus_tag="VBD11_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6953244.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT        14145 a         6554 c         8788 g        13513 t
ORIGIN      
        1 gattattaat gcttcttata acgcagctaa gcgtggtaaa gggattaata aagggtttgt
       61 tatttcattt atagctattg ggtcagggac tataatcact ttatctgtct tgattttttc
      121 aggaatttta aaatttgttc cgaatcaaat gattcctgta ggtggtatga ttattagtaa
      181 ttctatggta gcaatcggtt tgtgctataa acaattattg tccgaattta gaagtaaaca
      241 agaggaagtg gagacaaaat tagctttagg agcagatatt ttgccagctt cgattgatat
      301 tattcgtgat gttatcaaaa ctggtatggt acctacaatt gattcagcta aaacacttgg
      361 gattgtatct ttgccaggga tgatgacagg gttaattctt gctggaactt cacctatcca
      421 agcggttaaa taccaaatga tggtgacgtt tatgctatta gcaacgactt cgatagcttc
      481 ttttgtagct acttatttgg cttataaaat tttctttaat aatcgtaagc aattagtagt
      541 aacaaaagat tgattcattt taataaacag tatgttagtc tggtaagtat agtccaggct
      601 tttttggtat cttaatctta tttttgatcg aaaattgacc aagttattga gtcgattacg
      661 tttaaatgct actatttaat agaaattgtt gtagggatta tcaaaaggag gaagaaatga
      721 ttttaaaaat ttgtcgtgca gcatatagtt tacaatgggg aggtgtttac caattagctt
      781 tgctggatta tcctcgaatt aaggcgtttg aattggaaag gataggagct ttcatagctt
      841 acgagaaaca atataaaaga aaaactgaga tacaatgtga cgataaacat ctcctcgcaa
      901 aaattgttca ttttttaaaa tacaatagtt ttacttttcc ctatattccc aaatatagag
      961 aagcggcagc tacttttaat gaggatggta ttagtttaac ttctgatttt ttaagccata
     1021 catgtacgat tgaaactgca aaactaattt ttaaagaagg taaaatctta tcagcagtta
     1081 aagcctttaa taagcctgct gaagtactgg taaaagataa gaggaatgct gctggagacc
     1141 ctaaagatta ctttgactat gtgatgttga actggtcaaa taccaattct ggttatcgtt
     1201 tagtaatgga aagattgtta ggcaaagcac catctgaaca ggagttaaca gtaggtttta
     1261 agccaggggt cagttttcat tttacttatc aagatatcat caatcatcct gattctattt
     1321 ttgatggtta tcatcctgct aaaattaaaa atcagctttc tttagcagaa catttagttg
     1381 catgtgttat cccaaaacat tatcaagaag attatcaaag ccttgtgccc aatgacttga
     1441 aacacagggt ttattattta gattactgta acgaaacact ttatgagtgg aatcaaaaag
     1501 tttatgattt tctttgtcat ttggaaaata aataaaacca aaaagtttaa aactttttgg
     1561 tttaactgct attaattggt ctttaaaaga cttaaaaatt cttcaatcat aggactaaaa
     1621 gaagtatcat ttcggtaagc aataccaatc ggataaaaat tatttttatc cttaagtggg
     1681 acccagcata aatcttgaac ttggctaatg ctttcaaatt cacttggtaa aatagtgact
     1741 gcattgaggt cttgtaaact atgaataaga acttcccagt cattgtgttt aaagacgata
     1801 tgggggtcaa atcctaaagc acgacattta cgaggcaaca tctctcctag catataatgt
     1861 tcgttgagtg aggcaacttt ataatctctt aaatcattaa gttcaatttc aggaagtgtt
     1921 gcaagaggat gcgattttgg catgacaatg cttactttat atcctttagt tgaggtttgc
     1981 agtggttcga tagtgatatc attgcgggta cttggaaagg acaataagcc aatatcgatc
     2041 tttccttttg ctaacaattc ttgtaattta cgggaaccat cttgaatcaa agatacttca
     2101 acatttgaat gtgtagccat aaaagttgaa atctgtttca taaattgaat agcaaaaaga
     2161 gaagtaagcc ctacacggac accttccttt ttttcttgat ttaattgctg gatctccgta
     2221 accatttgat taacattagt catcaacaat tgtcctttct catagagaat ttctcctgct
     2281 tcagttaaag cgagttgacg accattttgt aagaataaaa tagtatttaa ttctgcttct
     2341 aattttttaa tggattgtga aagtgtaggt tgggtcacaa aaagactttt tgcagcgcgt
     2401 gaataatttt ttgcttctgc aacagcaata aagtaagtaa gttgtctaat atccataata
     2461 tctgtccttt tttcttagta atagtatagc acaagtctat caatctgacc tttatgatta
     2521 tttccacaat aatattagta aaagctatac ttatataatt ttttccaatt ttccttttgc
     2581 aaatagctat gatatagtta atatagttag tgaaatcaca aacaatcatt attagtagga
     2641 ggcatcttat gtcggatgtt gtcgaaaaac aaacaactaa atcgtttata atgaacgtat
     2701 taaatggttt agctttaggg actgttattg tcttgatacc aggagcaatc cttggagaat
     2761 taatgaaagc tttactgcca atgtggagcg gctttgcaac tttaatcgct gcaacagcag
     2821 ttgcaacaag tatgatgggg cttgttattg gtattatggt tggtctcaat tttaaattta
     2881 atcccattca atcagcatca ctaggattag cggttatgtt tgcaggtgga gctgcaacct
     2941 tccttaaagg cgctattatg ctaaaaggaa caggcgatat cattaacatg ggaattactg
     3001 ctgccttagg tgtccttttg atccaatttt tatctgacaa gacgaaatca ttcactttga
     3061 ttgtcattcc aacagtaaca ctcttactag ttggaggagt aggtcatgtg ttattacctt
     3121 atgtcaaaat gattaccacc atgattggac aagggattgc ttcattactt ggtttacaac
     3181 ctgttttgat gtcaatttta attgcgatga ttttctgttt cttgatcgta tctccaatca
     3241 caacagttgg gattgctttg gcaattagtt tgtccggaat tggttctggt gctgctaatt
     3301 taggtatctg tgctgctagt tttgggcttt gtatggctgg gtggtcagtc aattctaagg
     3361 ggacagcctt agcgcatgtt ttagggtctc ctaagatttc gatggctaat gtattagcaa
     3421 aaccgaagat tatgctacct atgatatctt cagcagcaat actaggaatt ctcggagctc
     3481 tctttaatat tcaaggaaca ccagctagtg caggttttgg tatcagtggt ttgattggac
     3541 cgattaatgc tcttaattta gcaaaaggtg gatggtcagt tatgaatatg ttattgataa
     3601 ttataatatt tgttgctgca ccaattatat tgaattttat ttttaattac ctctttataa
     3661 aagtacttaa aattattgat ccaatggact ataaattaga tatttaagga gatattagtg
     3721 atgaaactca aagtatttaa tgttagagaa gaagaagcaa cattagcaca agattgggca
     3781 aaccgaaatc atgtggaact ttctatgagt gaaggacctt tgacattgga aactgttaat
     3841 gaggtagaag gttttgatgg gattgctaat gcacagattg agcctttaga tgatgctatt
     3901 tatcctcttc tcaaagaaat gggaattaag caaatcgctc agcgcagtgc tggtgttgat
     3961 atgtataatc ttgagttagc taagcaacat ggtattatca ttagcaatgt gccaagttac
     4021 tcaccggagt ctattgcaga atttacagtg acaatcgctc ttaaccttat tcgaaaagtt
     4081 gagcttatta gagctaatgt tcgtgaacag aatttttcat ggaccttgcc aattcgagga
     4141 cgagtcctag gtaacatgac tgtggctata ataggaactg gtcgcattgg tctagcgact
     4201 gctaaaattt ttaaagggtt cggatgtcgt gttattggtt atgatattta ccataatcca
     4261 atggcagatg gtatccttga gtatgtcaat tcggtagaag aagctgttga agaagctgat
     4321 cttgtgtcct tacatatgcc accaactgct gaaaatacgc atctatttaa tttagatatg
     4381 tttaaacagt ttaaaaaagg tgctatttta atgaatatgg cacgtggagc attggtggaa
     4441 accaaagatc ttcttgaggc tttagatcaa gggcttttgg agggagctgg tattgatact
     4501 tatgaatttg aggggccata tatacctaaa aattgtcaag gtcaagacat ttcagacaag
     4561 gatttcctaa gactgattaa ccatccgaaa gtaatataca ctcctcatgc ggcttattat
     4621 acagatgaag cggtcaaaaa tctagtagag ggtgccttaa atgcttgtgt agaagtgatt
     4681 gaaacgggaa caactactac gaaagtcaac taaaagatgc ttaggcatct ttttttgtgg
     4741 ggtaacaaaa catatgatga ttatgacctt taaagcaaaa aaacatctta tgtatttata
     4801 gcttgaaagt cttttttata ataaaaaaag atatttttta taaaaatatt gaaaaaaaca
     4861 tcagatgaat agtattattt aatcataaaa gcgtttacaa aatacaagga gggttctctc
     4921 atggagaaaa caatggatat ggaacaacgt gaagaagttg tatcttataa ccgagcgaaa
     4981 gtatggcagt tagttatgtt tgccatgaac aatgcttcta caaatatata cttatttacg
     5041 tttatgtttg ttacctattt ctcaactggc gttcttggct cggcagccat ttttgtcagt
     5101 caaattatgg gttatatccg tatttttgat ggttttattg atccagctat tggtattatg
     5161 attgataaaa ctgatacaaa atttggtaag tatcgtccta ttttgattat tgggaacgtt
     5221 attacggctt tgtcattgat ttttcttctt gcattgagag gtgtcgatga aaatattcgt
     5281 ttcccattat ttatccttgt tctaattatc cataaaattg gatactcaat gcaacagaca
     5341 ataactaaag cagggcaaac agcactgaca aatgacccta agcaacgtcc tatttttaat
     5401 atcgttgatg cagttatgac aacatcatta atgacaggcg gtcagtttgt cgtttcagtt
     5461 ttcttagtac caaaatttgg taattttaca cctcaattct tcaatgtcct tatttttgga
     5521 acaattctta tttcagctat tttagcaatc gttgcaatta ttgggatttg ggcaaaagac
     5581 agaaaagaat tctttggttt aggagagaat acccaaaaga ctgctctaaa agattactgg
     5641 aaggtgttga aaggaaataa accgcttcag atattatcca ttgctgcagc tttggtaaaa
     5701 tttgccatcc aattctttgg cgattccgtt gttatggtgc tattgtttgg tattttattt
     5761 ggtaattatg ctttatcagg acaattctca cttcttttca ttgttcctgg tgttattatt
     5821 aatattttgt tctcaacaat tgctcgtaaa aaaggtcttc gtttttcata tgtcagagca
     5881 attcagatcg gtatgattgg tttacttgct tttggtgcag ttctttacgt tggtaagcct
     5941 ggtgacctta gtttaacaag cctaaatctt tatactattt tatttatcgt tactaatatt
     6001 attgcgagat acgcttcaca agcacctgct agtttagtat tgactatggg agcagatatt
     6061 tctgactatg aaacttctga atccggtcgt tatgtatcag gaatgattgg aactatcttc
     6121 tccttaacag attcgattgc ttcttcattt gctcctatgg tagtaggttt tgtgttagca
     6181 ggaattggat tttcaaaatc atttccaact atcgaaacac cactgccccc tgatttaaaa
     6241 atggcagcta ttagtatcct tgtggccatt ccatttatag ctttgtctat agctcttctt
     6301 ttaatgaaat tttacaaact agataaagaa gaaatggttc gtattcaaga gaagattcaa
     6361 gttatgaaag cagcaactac taaggaacgt gctaaagata ttgctcgtaa tgttcctttg
     6421 acagatatgg attatgttga tgtaacaaaa tatcctattg ataaagatta aaaagtttag
     6481 ctgctcttca atagagcagc tattttgtta aataagagct accattggag gattcttatg
     6541 gcaaaaatta tttcattagg tgaggttctt ttgcgattat ctccacctca atatcacaca
     6601 ctgatgcagg ctaatcacct aaaatgccaa ttcggtggtt cagaacttaa tgtcttagca
     6661 agccttgctc aactaggtta ccatgtagga ttggtctcag ctttgcctga taatgatttg
     6721 ggcaagatgg caagtcaatt tatattgtca cagcaaatta gtccagctgc tataattaaa
     6781 aaagaaggac gactaggaat ttattactat gaacaaggat tttcggtaag aacaaataag
     6841 gttatctacg atcgtaacta ttcatctttt tgggaaagta ccttgagtga ttatgatttt
     6901 acttctattt ttaagggagt ggattggttt catgtttctg gaattactcc agcactaacc
     6961 aaggacttat atgaggtgac tcggttcctt atgacaaaag ctaaagaagg aggtgtaaaa
     7021 gtatcgattg atctcaattt tcgtgagagt ttatggtcat cttttcaaga ggcacgtgag
     7081 caattatcgc cactattagg attattagat gtttgctttg gcttagagcc gatttattta
     7141 gctggcgaaa gtgaagattt aaaagatgag ttgggcttat ccagacctta ccttgatatt
     7201 gaattacttg aaaagataac ccaaaagatt gtccaagaat atgggttaga ctatattgcc
     7261 tttactcaaa gggaaatgga atatactaat cagtatatgt taaaatcata tttgtatcat
     7321 aataatatgt tatatcaaac tgataaaact ggcgttgaag tattagatcg tgtggggaca
     7381 ggagatgcct ttgctgcagg tttaattcat gcgcttctgg aaaaagaaac tcctcaaagg
     7441 gccttagaaa tagctatggc tacatttaaa tacaaacata ccattcaagg agatatcaat
     7501 atcatgacac gagatgatat tgcttacctc attgaaaaag aaacaaatga tattaaacga
     7561 tagtgtaaaa ggagagtgta tgttatatcc attattgaca aaaacaagaa atacttatga
     7621 tttaggcggt atttggaact ttaaattagg agaacataat ccaaatgaat tactaccttc
     7681 agatgaagtc atggttatcc cgacttcatt taatgatttg atggtaagta aagaaaaacg
     7741 tgattatata ggtgattttt ggtatgaaaa agtcattgaa gtacctaaag tatcagagga
     7801 tgaagagatg gtgctgcgtt ttggctcagt gacacatcaa gctaaaattt atgttgacgg
     7861 tgtattggta ggagagcata agggaggttt tactcctttt gaagttcttg ttcctgaatg
     7921 taagtataat aatgagaaaa ttaaggtatc aatttgtgct aataacgttt tagactatac
     7981 cacgcttccg gtcggaaatt atagtgaaat cattcaagaa gatggtagca ttaagaagaa
     8041 agtacgtgag aattttgatt tcttcaatta tgcaggtgtg catcgtcctc ttaagttgat
     8101 gatacgtcct aaaaatcata tttttgatat tacaattaca agtcgtctat cagatgattt
     8161 acagtcagca gatttacatt ttttagtgga gactaatcaa aaggttgacg aagttagaat
     8221 tagcgttttt gatgaagata acaaactagt gggtgaaacg aaggatagca gattatttct
     8281 tagtgatgtc catctttggg aagttttaaa tgcctatctg tacacagcgc gtgttgaaat
     8341 ttttgttgat aatcaactcc aagatgtcta tgaggaaaat tttggtctta gagaaataga
     8401 agtgacaaat ggtcaattcc tattgaatcg caaacctatt tattttaaag gatttggtaa
     8461 acatgaagat acgttcatta atggcagagg tttgaatgaa gctgctaact taatggattt
     8521 gaatcttttg aaggatatgg gggcaaactc ttttagaaca tcccattatc cttattctga
     8581 agaaatgatg cgtttagcag atcgaatggg agtgttagtg atagatgagg ttccagcagt
     8641 agggttattt caaaatttta acgcttcctt agatttgtca ccaaaagata atggtacgtg
     8701 gaatttgatg caaacaaaag cggcgcatga acaagctatc caagaattag tgaagcgtga
     8761 taaaaatcat cctagcgtcg tgatgtgggt agtcgctaac gaaccggcta gtcatgaagc
     8821 gggagcacat gattattttg agccattagt aaaactttat aaagatttag accctcaaaa
     8881 acgtcctgtc accttggtta atattttaat ggcaacccca gatagagacc aagtgatgga
     8941 cctggttgat gttgtctgcc ttaatcgtta ctatggttgg tacgttgacc acggtgattt
     9001 aacaaatgca gaagtaggta taagaaaaga gttattagaa tggcaagata aatttcctga
     9061 caaaccaatt atcataacgg agtatggcgc tgatacgtta ccgggattac attctacttg
     9121 gaatattcca tatacagaag aatttcaatg tgatttttat gaaatgagtc atcgtgtttt
     9181 tgatggtatt cctaatttag ttggtgagca agtctggaat tttgtagact ttgaaactaa
     9241 tctgatgata cttcgtgtac aggggaatca caaaggtctc ttttcaagga atcgccagcc
     9301 gaaacaagtc gtcaaagaat ttaaaaaacg ctggatgact attcctcatt accataataa
     9361 aaaaaatagt gtaaaatagg ggtagttatt ttagaagagg tgatgatatg gctagaccct
     9421 tagtagaaca aacagcagat cgtctattgc atttaatttt agaaagagaa taccctgttg
     9481 gtgctaagct ccccaatgaa tatgaattag cagaagatct agatgttggt cgcagcacaa
     9541 tccgagaagc agtcagaagt ttagcaacgc gtaatatttt agaagtcaga cagggctctg
     9601 gaacatatat tagttctaaa aagggtgttt ctgaggatcc attagggttc tctctaataa
     9661 aggacacaga taggttgaca tcagatctct ttgaactacg ccttttattg gaacctcgaa
     9721 tagcagagct ggtagcttat agaattacag atgatcagtt acaattactc gaaaagcttg
     9781 ttggagatat tgaagatgct gttcacgcag gcgatccgaa acatttactt ttagacgtgg
     9841 aatttcattc catgctagct aaatacagtg gtaatattgc aatggatagc cttttgccag
     9901 tcatcaacca gtcaattcat ctgataaatg cgaattatac aaatcgtcag atgaagtcgg
     9961 atagtttaga agcacataga gaaattatca aagcaatccg agaaaaaaat ccagtagctg
    10021 cacatgatgc tatgcttatg catatcatga gtgttagacg ttcagcgttg aaagcttaat
    10081 ataagaagaa aaatagctat tcttgaatag ctattttttt agtattcttc ttgacaattc
    10141 atcagatgac ttatttaata tagttaaaga taacggttac attttgggag gtattatgtt
    10201 aaatcaattg aaaaacaact atttctttgc tgttattcga gggaaatcat ctgaggatgc
    10261 gctcgaaatc gcaaagcatg ctattttggg tggtattcgg aatattgaag taactttttc
    10321 gactcctgaa gcaagcaaag tcatcaaaca attatcagat gactttaaaa ataataaaga
    10381 aattattgtg ggggccggaa cggtcatgac aactgaactt gctaaagagg ctattgatgc
    10441 tggagctaaa tttttggtta gcccacactt tgattcagat attgctaatt tagctaatga
    10501 aaacaaggtt tattatttcc caggctgtgc aacggcaaca gaaatcgtgg tagctagaaa
    10561 gtataaatgt cagattataa agctttttcc aggtggagtt gtgggtccag gttttatcaa
    10621 agatattcat ggtcctattc cagatgttga tttaatgcca tcaggtggcg tctctgtttc
    10681 aaatgtagtt gaatggagaa aagcaggagc agttgcggtt ggtgttggct cagcattatc
    10741 tagtaaagta gccacagaag gctatgatag tgttactaaa atagctaaac agtttgtttc
    10801 tgcacttgat tagtgagtaa gagaagaata tggcgtttaa tactgaaaca tttatgttaa
    10861 aaaatcaagc agctatacag ctgtatgaag aggtcaagag acaacctatt tttgattatc
    10921 attgccactt agatccaaag gacatttttg aagatcatat ttttgataat attgtggatt
    10981 tgtggcttgg tggtgaccac tataaatggc gtcttatgag ggctaatggt atttctgaag
    11041 cagaaattac aggtccagca tccaaccttg agaaatttaa agcatttgct agaacactag
    11101 aaagagcata cggtaatcct gtttatcatt ggtctgctat ggaattgaaa aatatctttg
    11161 gtgttaatga gattttgaca gaatcaaatg ctgaggaaat ttatcatcgt ttaaatcatt
    11221 tcttgaaaga gcataaaata agtcctagac gtttaattgc agatagcaaa gttatgttta
    11281 ttggtactac agaccaccct cttgatactc tcgagtggca taaaaagttg gctgctgatg
    11341 agagttttaa aacagttgtt gctccgacat ttaggccaga tgaagctttt attgaacatc
    11401 gacattttgt tgattttatt actaaactgg gtgacattac tcaaaaagag attacagatt
    11461 tttcaacatt tatagcagca atggaagaac gcattgcgta ttttgctcaa aatggttgta
    11521 gggctagcga tatcagtttt acggaaatcg tctttgaaca aactgatgaa ttggagttga
    11581 acgatttatt taataaagtt tgtgaaggct atatcccaaa tcaatcagaa atcagcaaat
    11641 ggcaaacagc tgtttttatg gaactttgcc gattatataa aaaatatggt tttgtaacac
    11701 aggtgcattt tggagctttg agaaataatc attctactat ttttgaaaaa ttgggagctg
    11761 acgtgggtgt ggattcactt ggtgaccaag tggctttaac tgttaatatg aaccgtttat
    11821 tggattcctt ggttaaaaaa gatagtctgc ctaaaatgat ttggtataat ctcaatccag
    11881 cttacaatat agcagtagct aacactttgg caaattttca agctaacgaa cttggagtga
    11941 gatcttacct tcaatttggt gcaggttggt ggtttgcaga tactaaatta gggatgatta
    12001 gtcaaatgaa tgccttagca gagcaaggca tgttggcgaa ttttattggt atgttaacag
    12061 attctagaag ttttctatct tatcaacgtc atgactattt tcgacgtatt ttatgtacct
    12121 acttaggaga atggattgaa gaaggagaag ttccagaaga ttatcaagct ttaggaagta
    12181 tggctaaaga catcgcttat caaaatgcag ttaattattt taagaactaa aaaggaagag
    12241 tgaaatcatg gaaatgtcat ttaggtggta tggtgaagac gaccccgtta ctttagaaaa
    12301 tattggtcaa attccaacaa tgaaagggat tgttacagcg atatacgatg taccagtagg
    12361 agaagtttgg tcacgtgagc gcattcaaca gctgaaagaa aaggtagagg cagcagggct
    12421 taaaatttcg gttatcgaat cagtacctgt tcatgaagat atcaaattgg gacgtccaac
    12481 acgtgatctc ttgattgata attatattca aaccgtcaaa aatttggctg cggaaggtat
    12541 tgatactatt tgttataatt ttatgccagt atttgactgg acacgcactg atttagcata
    12601 tcaatatcca gatggttcca cagctttgat atttgatgag acggtttcta aaaaaatgga
    12661 tccagtaaac ggtgaattat ctttgccagg atgggatgcc agctatagta aagaagaaat
    12721 gaaggctatc atggatgcct atgctgaaat tgatgaggaa aaactttggg aaaatctcac
    12781 ttattttatt aaacgtatta ttcctgaagc ggaagccgta ggcgttaaaa tggccattca
    12841 tccggatgac ccaccatatt ctatttttgg tttacctcgc attattactg ggttagaggc
    12901 tattgagcgt tttgtgaaat tgtacgatag taaaagtaat ggcatcactt tatgtgtggg
    12961 ttcttacgct tctgatcccc agaatgatgt tttagagatt tcgcgacgtg cttttgagtt
    13021 agatcgtgta aactttgttc atgctcgcaa tattaaatta ggtgatggaa agtcatttaa
    13081 agaatcggct catccatcag aatacgggtc aatagacatg tatgaagtta ttaagttatg
    13141 ccatgaattt gggtttgagg gtgctattcg acctgaccac gggcgtatga tttggggaga
    13201 aacaggtcgt ccaggatatg gcctatacga tagagcttta ggagccactt acgtttctgg
    13261 tttatacgaa gctgtgatta aaggttcaaa ataaggcgat gcttccaaat ttctcctgtt
    13321 ccaatctcct gaaagagaaa tttggaaagt tattatcata atatgattgt acttagctaa
    13381 gagtagaata cttatagaag ttaatgagag gaataagatg tcaaaaacaa ttacatttac
    13441 caataaagtt gtcgtcataa caggtgcagg cggtgttctc tgtggttata tggctaaaga
    13501 atttgcgaaa gcaggcgcta aagtggcatt attagatttg aatcaagaag ctgctcaaac
    13561 atttgctgat gaaattgtag aagaaggtgg tatcgctaag gcttataaag ctaacgtttt
    13621 gtctaaagaa aaccttgaag aagtgcatca agccgtttta gaggatcttg gaccaactga
    13681 tattttagtt aatggtgcag gaggcaacaa tcctaaagct acaactgata atgaatttca
    13741 cgaattagac ttaccttcag aaacaaaaac cttctttgag ttagatgaag ctggtattag
    13801 ctttgttttt aacttaaatt atttaggaac attattgcca acgcaagtat ttgcccaaga
    13861 tatggttgga cgtgaaggag cgaatattat taatatctct tcaatgaatg ctttcacccc
    13921 tctgaccaaa ataccagctt attctggagc taaagcagct attagtaact tcacacaatg
    13981 gctagctgtt catttctcaa aagttggtat ccgttgtaat gctattgcac ctggtttctt
    14041 ggtaacaaat cagaaccgaa gcttgttatt tactgaggac ggtcagccaa ctgcgcgtgc
    14101 tgagaaaatt cttaataata caccaatggg aagatttgga gaagcaagtg agctgattgg
    14161 cggattgttc tttttagcag atgaaaaatc atcaagtttt gttaatggcg ttgtccttcc
    14221 gattgatggt ggatttgctg cttactctgg tgtttaattt acaagataat agccacttaa
    14281 cacataagag attattgagt tgagtggttt tttaaggaaa tttgtttaat gatcttacca
    14341 ctaaatgatg ggtaggttgc taattggaaa atcctcataa gttagataaa tttagaaact
    14401 ggagataata tggtagatac aaatcacaaa atcgcttttt gcatagattc tgatggttgc
    14461 gcaatggata caatgacata taagcatcaa ctcttttttg gtccaattgc tgcagatata
    14521 tttgggatat cagaacaaga aagaacaagt tttttaaaag agtgggatag agtcaatctt
    14581 tattcaagaa ctcgtggagt taatcggttt gtgggattgg ttatgggcct tgagtttgcc
    14641 ggagtagaac atattgataa attaaaagaa tgggtcgaaa caacagactc attgtccaat
    14701 aattccttag agttagcgct tgccaacgat tttagtagag atttagagaa ggctttagaa
    14761 tggtccaatg aagtgaatcg ccaaatcaaa aattatgagg gagattcttt agcgttttca
    14821 ggtgtgttag aaagtttaga acgattaagt caatttggta atgtttatgt cgtgagttct
    14881 gctaacaagg aagctgtttg gcaagagtgg gaagaacagg gattgctaaa gtatgttgat
    14941 gatatctatt gtcaagatag aggtaaaaaa gaagatgtta tcgccacttt aattgcaagt
    15001 ggttactgcc gtgaaactat tttgatggta ggagattccc tgggtgatct ggctgcagca
    15061 gaacaaaatg gtattgcatt ttacccagta cttgttggta aggaagtcaa atcatgggaa
    15121 attcttagag aagatattgg tgaagctttt gcaaaaggtc aattcgaaca gcaaaggcaa
    15181 aaagaatcta ttaatacctt ttgggctaat ttagataact aatagaaagg ataggctatg
    15241 acacatttag ttgacttaac aaagaaaccc ttcaatctaa atcaagaagc aattgagtgg
    15301 attgaaaaaa caattaatga gatgacattg gatgagaaaa ttgggcaact ttttttcaat
    15361 atgggagcta gtcgttcaga agaatattta acagatgttt tggatcggta tcatattgct
    15421 gctgttcgct ataatagagg tagttcaagt gaaatctatg atcagaattt aattttacag
    15481 acaaagtcga aattgccgat gttgattgct gcaaatacag aagctggtgg agacggtgct
    15541 gtcacggatg gaactaaagt tggcgatgaa ataaaagtgg ctgctacaaa tgatccgaaa
    15601 tatgcttatg aaatgggaag aatcgcaggg atggaggcat ctgctgtggg ttgtaatgct
    15661 tcattttctc caattgtaga tttgacacgt aattggcgta atcctatcat agctagtcga
    15721 aattggggtg ctaacgttga tcaaattatt tcactttcaa aagagtatat gaaaggaatc
    15781 atgcaatata atattgtacc tttcgctaaa cattttccag gggatggcat tgatgagcgt
    15841 gatcatcatt tgtcatttgc ttccaatcct atgagtaaag aggagtggat gtcgacattt
    15901 ggccgcattt atggtgaatt ggctgacgca ggtttaccag gagtcatggc aggtcacata
    15961 catttaccaa acgttgaaaa agaaatgcat ccagagcgag accttgatga tatgttacca
    16021 gcttcattga ataaaacact tttagatgag ttattgagag gagagttagg ttataatgga
    16081 gctattgtaa cggacgcttc tcatatggtt ggaatgactg cttcaatgcc aaggagagat
    16141 ttgttgccaa cggcgattga agcaggttgt gatttattct tattctttaa tgaccctgat
    16201 gaagacattc aatggatgaa agaaggttac gaaaaaggta ttctaacaga ggaacgtctc
    16261 catgacgctt tacgtcgtac tctcggtcta aaagcgaaat taggcttgca taactatgag
    16321 ggacgtcgcc aagaactctt tatgcctaaa gataaggcta tggctttaat caacacatta
    16381 gagtcgcaaa aaatagctga tgaagttgct gataaagcag taactctggt aaaagataaa
    16441 caaaaagata tctttccagt aaatccagaa cgttatcgtc atatcttact tgttaacgtt
    16501 gaaggatata agggaggttt tggtgcgatg attgctggaa acaagcaacg tgcatcggat
    16561 tacatgaaag agttacttga ggcacgtggc catgaagtaa ctgtctggga gtctaccgaa
    16621 gagcgtatta tgaaattgcc acaagaggag cgggcagcag ctatcgctaa tgtttacgca
    16681 caaaaacagc caatcgcaaa tctaacagaa cactatgact tgattattaa cttggtagat
    16741 gtcaatgctg gaggaacaac acaacgtatt atttggccag cagctaaagg cacccctgat
    16801 cagccttttt acgttcatga aataccatct attgtgattt cagtacaaca tgcatttgct
    16861 ttagcagata tgccacaggt agggacttat attaatgctt atgatgggct tcctagcacg
    16921 ataagtgcag tagttgcgaa attggctgga gaatcagaat ttacaggagt ttcaccagta
    16981 gatgcttact gtggtttaat tgatacacat ttatggcgcc aatgctaata gaaacaagat
    17041 gatgtacttt tatgtaacat aaaaaaagag cttaaagctc tttttttatc cttcaaaata
    17101 gagtagttcc ttgggtgttt tggtaaatac ttcaaagccg gttttagtca cataaccgca
    17161 gtcttcaatt cgcacaccaa ctttatctgg tatataaata cctggttcaa cagagaagca
    17221 catgccttct tgaatctcca tatcgttacc agccataata gatggaaatt catggacatc
    17281 catacctaat ccatgaccaa ggcggtggtt aaagtattgt ccataaccag ccttttcaat
    17341 aacatttctt gctgctgcgt caacttcaga agcaaggact cctggcttaa taaaatcaat
    17401 agccgcttga tgtgcttcaa gacataaatg gtaaatatct tttttgaatt gatcaggttt
    17461 ccccactgct actgtacggg tcatatctga cgtataacct agtgtttcta cacctaaatc
    17521 aaataataac aaagcattat tttcgatttt attagttcct gggataccat gtggatttgc
    17581 tgcattattg ccagtcaaaa ccatagtgtc gaaactcatt ttattgatac cttgtttttt
    17641 catttcaaat tcgatttgag cgataatatc ggtttcagtg ttgtttagcg agatattgtc
    17701 aaatccaact tgaacagctt tatcagcaaa ttctccagcg actaacattt tctcaatttc
    17761 atctcgtgat ttaattaggc gcatgttttg aatataaggt gttaggtttt caaaatgacc
    17821 atcaaaaacg gtttggagac cttggaattt agtaacatta agattatcaa attcggctag
    17881 aactttagaa acagaaaagg atggtaagtt gctagctatt ttttgccaag gattttctga
    17941 atctatgtaa ccaaagacgg gaaatggtac actttgtttt gcacgcgata cttctagtgc
    18001 tggtacaaat aaaataggat cgcgatcttc atagacaaat aaaaacattt gtctttcatg
    18061 gggatcgcaa aagaaacctg ttaaatagtt gactgtcaca ggatcagaaa agacagcaag
    18121 ttcagcttgg actgaatgta agtgatggcg tatacgatta agttttgaca taataaccct
    18181 tcttcctaaa aggaacttag tctagttagg ctagtttctt atgcctatta tttttccaaa
    18241 aaaataggaa aaaatcaagt ttagttagta ttttgaaagt ttttcataaa aaagctttat
    18301 aaaagcttga aagtgtttcc aaaaggtgat aaaatatcag tgaaagcgtc attttcaaat
    18361 agaaaggaag ttaaatcaat atgaatacag atgatacgat tacgatttat gatgttgccc
    18421 gtgaagcagg tgtttcgatg gcaactgtta gtcgtgtggt aaatggaaat aaaaatgtca
    18481 aggaaaatac acgtaagaaa gtccttgaag ttattgatcg tcttgactat cgtccaaatg
    18541 ctgtggcgcg tggtttagca agtaaaaaaa cgacaactgt tggtgttgtc attcctaata
    18601 tcgcaaatag ctacttttca attttagcgc gtggtattga tgatattgca gcaatgtaca
    18661 aatataacat tgtccttgct tcaagtgatg aagacgatga taaagaggtt aatgttgtta
    18721 atactttgtt tgctaagcaa gtagacggta ttatttttat gggacaccac ttaactgaaa
    18781 agattcgtgc agagttctct cgttcacgca caccgattgt tttagcaggt actgtggacc
    18841 ttgagcatca attaccaagt gttaatatcg attacaaagc agcagcagtt gatgttattg
    18901 atattttagc tggcaatcat aaagacattg cttttgtatc aggaccactt attgacgata
    18961 ttaatggcaa ggttcgtttg gcaggataca aagaaggtct caagaaaaat ggcttaaact
    19021 ttaaagaggg gttggttttt gaagccaact accgttatgc tgaaggcttt gcattagctc
    19081 aacgtgtgat taatgctgga gcaacagcag catatgttgc tgaggatgag ttagcggcag
    19141 gtttgttaaa tggtcttttt gaggcaggta aacgtgttcc tgaagatttt gaaattatca
    19201 caagcaatga ctcgccaatc gcacaatata cacgtcctaa cttaacttct atcagtcaac
    19261 cggtctatga tttgggtgct gttagcatgc gtatgctaac aaaaattatg cacaaagaag
    19321 agttggaaga aaaagaaata gtgcttaatc acgggattgt taaacgtggc acaacaaata
    19381 attagtgtta gagtatgtta tttgaaaaat agtggtagaa tggtagtagc ttagcgctta
    19441 actttctgat acttaacatg atatattgag aggattaggg tagcttagct tcctaatctt
    19501 tttttagagg tgtaatatga caaatgaatt gataatgcaa gcttttgagt ggtatttacc
    19561 tagtgatggg aatcactgga agaaattaga ggagtctata tcagacctta aaaaacttgg
    19621 aattagtaaa atctggttac caccagcatt taagggaact agcagtgatg atgtaggata
    19681 tggtgtttat gatctctttg atttaggaga atttgaccag aatggaacaa ttagaacaaa
    19741 atatggtagg aaagaagagt atctaaagct tattaagtcg ttaaaggcaa atggcattaa
    19801 accgtttgca gatatcgttc ttaaccataa agccaatggt gatcataaag aaaaatttca
    19861 agtcatcaaa gtcaatcctg aaaatcgtca agaagcatta agtgaaccct atgagattga
    19921 aggatggacg ggatttgatt tcccaggtag acagggtgag tacaatgatt ttaaatggca
    19981 ctggtaccac tttactggtt tagattacga tgctaaaaat aatgaaacag acatctttat
    20041 gattgtaggc gataataaag gttgggcaga cgatgatttg attgatgatg aaaatggtaa
    20101 tttcgattat ctcatgtaca atgacattga ttttaagcat cctgaagtta taaaaaactt
    20161 acaagattgg gctaagtggt ttattgaaac tacaggcatt gaaggttttc gtttggatgc
    20221 tgtgaaacat atcgattctt actttattca aactttcatt aatgatattc gtacaaaaat
    20281 aaaacccgat ttagaggtct ttggcgaata ttggaaatct gaccaaacat caatgaagga
    20341 ctatttagag gcaacacaat ttcaattttc tcttgttgat gtcaccttgc atatgaattt
    20401 ttttgatgcc agtcaccaaa accgtgattt tgatatgcga acaatttttg atgatagtct
    20461 tgttattgat aatcctgagt atgctgttac attcgtagaa aatcacgata cgcaaagtgg
    20521 acaagctcta gaaagtagag tggaagattg gtttaaacct ttagcttatg gcttgatact
    20581 tttacgccaa caagggactc catgtctttt ttatggtgat tactatggca ttcaaggaga
    20641 atttggccaa ccatccttta aagaggttat tgataaaatg gctgagctac gccaaaacta
    20701 tgtatttggc gaacaagtcg attactttac gcattcaaat tgtattggtt ggacttgtct
    20761 tggggatgag gaacataatt cttgtttagc tgtggtccta acaaatggtg atcagggatg
    20821 gaaacatatg gaagtagggg aaatttatgc aggcaaaact tttgttgatt atttaggcaa
    20881 ttgtgaacaa gaggttgtta taggagatga tggttggggt gattttttag ttgcatctgc
    20941 gtctatctcg gcttgggtcc ctaaaataga ggaagctaac tgataaatca ggtgaaaaaa
    21001 aaggtaaaat gagatataat aagacctatg aaagttttac tgtatttaga ggctgaagaa
    21061 tacttgaaaa agtcaggtat cggtcgtgct atcaaacatc aagagaaagc tcttcaaatt
    21121 gctgggatag attataccac taatcctaca gatgactttg accttgttca tatgaatacc
    21181 tatggtatta gaagttggct tttgatgagt aaagctaaaa aaactgggaa aaaagtaatt
    21241 atgcatggcc attccactga agaggacttt agaaattctt ttattggctc aaatttagta
    21301 tccccgcttt ttaaatggta cctttgccgt ttttatcaaa aagcagatgc cattataacc
    21361 ccaactgatt attctaaaca actaataaaa gcatatggca tcaagaaacc aatttttgtt
    21421 ttatctaacg ggattgatct aagtcgctat cagaggtcag aaaagaaaga agctgcattt
    21481 cgtcactatt ttcacttaag taaggatgat aaagttgtta tgggggccgg tctttatttc
    21541 atgcgtaagg gaattgacca atttgttgaa gtagctgcaa aaatgcctga tattcgtttt
    21601 atttggtttg gagaaacaaa taaatgggtt attcctcgaa aggttaggca aatcgttacc
    21661 aagcaacatc cctcaaatgt tacttttgct ggttacataa aaggtgatgt ttatgaaggt
    21721 gccatgagtg ctagcgatgc tttcttcttt ccgagtcgtg aagagacaga agggatagta
    21781 gttttagagg ctttagctag tcatcagcat gtggttttaa gagatatccc tgtgtatcac
    21841 ggttgggtga ccgaagacag tgttgaatta gcgactgatg ttgatggttt tgttgaaaag
    21901 ttggataaag tcttatctgg taagagtgat aagattaaag aaggttatcg tgtggctgag
    21961 agtcgaagta tcgaacgaat tgcccatgaa ttggcaagtg tttatcaaaa agtaatggag
    22021 ttgtaagatg cgtataggtc tatttacaga tacatacttt ccccaagttt cgggagtttc
    22081 aacaagtatc cgaactttaa aggaagggtt ggagaaggaa ggacacgaag tttatatttt
    22141 cacaactacc gaccgtaatg tcaaacgttt tgaagaccct acaattattc gtttaccaag
    22201 tgtccctttt atttctttta cagatcgtcg agtagtttat agaggtttaa tttctgctta
    22261 taggatagct aaagactacg aacttgatat tattcatact caaacagaat tcagtttagg
    22321 acttttagga aaattagttg caaaagcttt aagaatacct gttgtgcata cttatcatac
    22381 tcaatatgag gattacgttg gttatatcgc aaaaggcaaa ttgattaagc caagtatggt
    22441 caaatatatc atgcgtacct acctaagtga tttggatggt gtcatatgtc cttctcgaat
    22501 tgttctcaat cttctagatg gttacggtgt caaaattcca aaacaagtta ttccgacagg
    22561 tattcctgtt gagaactatc gtcgagagga tatctctgaa gaaacaatca agaatctacg
    22621 aacggagctt ggccttgcgg ataatgatac aatgctattg agtttatcac gggtatcttt
    22681 tgaaaaaaat attcaagcaa tcttgatgca tttatcagct gtcgttgatg aaaatcctca
    22741 cgtaaaatta gtgatagttg gagatggtcc ttatctttca gatctaaaag aacttgttca
    22801 ctctttagaa cttgaaaatt cagttatttt tacaggtatg gttgagcata gccaagtggc
    22861 tatctactat aaagcatgtg atttctttat tagtgcttca acaagtgaaa cgcaaggatt
    22921 aacttacatt gagagtctgg cgagtggacg tccaatcatt gcgcaaagca atccttatct
    22981 agatgatgtt atttcagata aaatgtttgg aactctgtac aagaaggaaa gtgatttggc
    23041 agatgctatc ctagatgcaa tagcagagac acccaaaatg actcaagaag cttatgaaca
    23101 gaaattatat gagatttctg ctgagaattt cagcaagtct gtatatgctt tttacctaga
    23161 ctttctgatt tcacaaaaag ctagtgtgaa agaaaaagtc tctctgacga taggaaataa
    23221 ggatagccat tctactcttc gttttgtcag aaaagcggta tatttaccta agaaagtttt
    23281 tacctttaca ggaagagcct ctaaaaaagt ggtaaaagca cctaaacgtc gtatttcatc
    23341 tattcgagat tttttagatt gagacgaata aaattagtaa atgtcttgaa attataaaaa
    23401 atattgttat aataaaaaat gaagacatgt taggttctaa tagatttcaa gagcgagcac
    23461 tggttggtgg aaagagtgca attagagaac ttagtactac ttccttgatt ttatgtaaac
    23521 ataaaagggt ggttccctta tcgccactag aggtctgaca caagtatact cgagtttttg
    23581 tgttggataa attgaggtgg aaccacgtag tctttacgtc cttttggatg taaaggctct
    23641 ttttttataa aagaagtggt tgcataaaag aggcagattg cttttctaat gttatactta
    23701 ttatccctat ctaggataat gtggaattca atcaaaggtc aatccctttg tttattagtg
    23761 aattaatgtg tttaggaatt ggtatcaccc taacagttat taagaaaagg agaacaagat
    23821 gattaaaatc actttcccag atggtgcaat tcgtgaattt gaatctggca tcacaacatt
    23881 tgaaattgca caatcaattt ctaactcttt agctaaaaaa gctttagctg gtaaatttaa
    23941 tggtcaactt attgatacaa cacgtgctat cgaagaagat ggtagtattg agattgtaac
    24001 acctgaccac gaagatgctt taggagttct tcgtcactca gcagcgcatt tatttgctca
    24061 agctgcaaaa cgcctttttc cagacttatg tttaggtgta ggtccagcta ttcacgatgg
    24121 tttttactac gatactgata ataaatcagg tcaaatttca aatgatgatc ttcctcgtat
    24181 tgaagaagaa atgaaaaaaa ttgttaagga aaatcaccct tgtattcgtg aagaaatcag
    24241 taaagaagaa gcgttagaac tttttaaaga tgacccttat aaagttgaat taattagtga
    24301 acatgctgaa gatggtttaa ctgtttatcg tcaaggtgaa tttgtagacc tttgtcgtgg
    24361 acctcatgtc ccatcaacag gacgtattca agtctttcat cttctaaatg tcgctggggc
    24421 ttactggcgt ggtaacagtg ataatgctat gatgcagcgt gtctatggta ctgcttggtt
    24481 tgataaaaaa gatttgaaag catatcttaa acgccgtgaa gaagctaagg aacgtgacca
    24541 ccgcaaatta ggtaaagaac ttgatttatt tatggttaat cctgaagtag gtcaaggatt
    24601 gccattctgg ttgccaaatg gagcaactat tcgccgcgaa ttggaacgtt acattgttga
    24661 taaggagatt gcttcaggtt atcaacacgt ttacacacct ccgatggcat ctgttgaatt
    24721 ttacaagact tctgggcact gggaccacta tcgtgaagat atgttcccaa caatggatat
    24781 gggagatggg gaggaattcg ttcttcgtcc aatgaactgc ccacaccata ttgaagttta
    24841 taaacatcat gttcactcat atcgtgaatt gccaattcgt atcgctgaac ttggtatgat
    24901 gcaccgttat gaaaaatcag gggccctcac aggattgcaa cgtgtacgcg aaatgacact
    24961 caacgatgcc catattttcg ttacacctga gcaaattaaa gatgaatttc ttaaagctct
    25021 taatctcata gcagaaattt atgaagattt taacttaaca gattaccgtt tccgcttatc
    25081 ttatcgtgac ccagaagata aacataagta ttatgataac gatgagatgt gggaaaatgc
    25141 acaagctatg ctaaaagagg ctatggatga ttttggatta gattattttg aagcagaagg
    25201 cgaagcagcc ttttacggtc ctaaattgga tatccaggtg aaaactgctc ttggtaatga
    25261 agaaacatta tctactatcc aactagattt ccttcttcca gaacgctttg atcttaaata
    25321 catcggtgca gatggtgaag aacatcgtcc aattatgatt caccgcggtg gtatttcaac
    25381 aatggagcga tttacagcta ttttaattga aacttacaaa ggtgcctttc caacttggct
    25441 tgctccacaa caagtttctg ttattccaat ttcaaatgaa gcccacattg attatgcttg
    25501 ggaagtggct cgtgccctta aagatcgtgg tattcgtgct gaagtggatg accgtaacga
    25561 aaagatgcaa tataagattc gtgcggcgca aacccaaaaa attccatatc aattgattgt
    25621 tggtgataag gaaatggaag aaaaagccgt aaatgtgcgt cgctatggaa gtaaagcgac
    25681 tgaaacaaaa tctatagagg aatttgtaga aagtatttta gctgatattg caagaaaatc
    25741 tagacctgac gaagttaaat aaattttatt agtcattata aaaatcatta ataaatgatg
    25801 tgaactgatt actgtatgat gcagaatcag ttttttagat gctaattttt tcttgatgtt
    25861 taattataag ctaattatag ataaataatc tcatataaca ttcacaaaat tagatttttt
    25921 ggtataatta atatgattga cacataaagg aaaatagtat taataaaaag gggtatggag
    25981 cattgcgaac agtattagta gtgcaaggtg atgacgaaac aatagagtta ttaaggtcgt
    26041 atttagaggg agctctttat aaggttgtga tggctagtga tggtgaggag gctttttctt
    26101 tatttcagca acatcagatt gatttagcaa tcatagatat tacgctacct aaaatagatg
    26161 gatatgagtt gacgcgactc attcgtcaag atagtcagat tcctattatt atgctagcag
    26221 ctaagacaac agatatggac agaatattag gattgaatat tggagcagat gattttatca
    26281 cgaaaccgtt taattctttg gaggttttag ctcgaattaa ttcgcaactg cgtcgttact
    26341 atgaattcaa tagtcttgcc aaacctaaaa atcaatttat aaagataggg gaattggaac
    26401 tagatgagga acatgttgaa ctaactaaaa atggtaaaca tatcaagttg actgctacag
    26461 aatttaagat tcttcatatc ttaatgtcat caccaggaaa gatttatact aaaacacaac
    26521 tctatgagaa gattaatggt aggtatttag aaggtgatga gacaacgatt atggtgcata
    26581 tttctaatat ccgtgacaaa atcgaagatg atagtaaata tcctaaatat ataaaaacac
    26641 ttagaggagt agggtataaa atcgaaaaac cgcagtatct tgtttaaaaa cataatggct
    26701 agtgagagcc attttttagt gaagatattt cttggtttta agctttcctt tttgctataa
    26761 taagtaaaat tggaggggag agatatggca aagaccgctc ttaatattcc ttttacagtt
    26821 gaagggatag aaaattatta tcaaaatata aaaatgatac tgtctttagc agataaatgg
    26881 agcataccta aagatgatct attactcttt ttaaagggac tcaaggctat tgatgatata
    26941 aaagttggct ctcctatttt taagtatttt tggacttctt taagtttaca agcacccctc
    27001 aaagctttag aattcgtgtt agagcaggct aaaatgccaa ctgaattgtc aggagaatta
    27061 tcagaaacac aatacttggt cgcacagttt tcagatgagt tagctccgca tgatgatttc
    27121 tggatagcct tatcacaagt tatttatgat tcttttcctg ggaatagtct agccgaagat
    27181 actgtattaa ataggaaact ccatcaattc cgctatttga tttctagtca gcaagcacag
    27241 tatgttcgac gatactttaa agatgttggt atgactgata gagatgcttt agtaaactac
    27301 ctatcatgtt taagagaacc agatagtatc gcctactatg aatctgctcg cttgcataat
    27361 aaacgtcgga gaaatggtga aatctttggt ttcccagatg atgagccggt tattaatagt
    27421 aagcttttaa tttcattcca tacggaattt attattgatg acaaaggaaa ctttttaaac
    27481 gagattgacg cagaagtaat cacacgtaat ggcattatca acggtgctag ctttaattat
    27541 gcttttaaaa ataatacacg tcataaagaa ttagatgttg atcctgtaaa gttagaccca
    27601 aaatttcgaa acgatatgac tagaggttat agatcgccaa atctatctag aaggaaatgg
    27661 tttttcttta aagaagagga ttacgattgt agttatttca ataaaaaagg atattacgca
    27721 tttggaaggc gtagtgctaa gcaatcggtt gataaacaag tcaaatactt gaagaaggct
    27781 gtgcagaaaa tgcgcatcaa ctgatacata tactattaaa agataccatg atactatggt
    27841 atcttttaat ttgcggtatt aatgtcaaat ggtttttatt gaccacatat ttttataaac
    27901 gagtggtcaa aattgaaaaa gtgtctagct tatgttaaaa tacaattgaa taatagaaag
    27961 aaggtgctgt atgattaaga aaaataaagt ttttttagga gttcttttag tccttgttgt
    28021 tatccttgga ggtggtgtat tattttacca atcacaattc caaaagacaa ctaatcaagc
    28081 gttagctatt gcttataaag atgctaaagt ggctaagaag gatgtcatac atcaaaaaat
    28141 tgacaaagaa tttgaaaact ttagagggag ctacgaaatt gaattcaata ccaaaagcgc
    28201 agaatacagc tatcatgtag atgctaagac tggacaaatc cttgagcggg acatggataa
    28261 taatggcttt tcaaaatcga cttctcagtc aagttcatcg tcaagtcaaa aatctcataa
    28321 aataagtcaa gaagaagcca aaaagattgc atttaaagat gctaatatag aggaatcaga
    28381 agtcagtaat cttaagatca aagaagaaat tgaaaatgga aaatcagttt atgatattga
    28441 ctttgtggac ctgaaaaata aaaatgaagt tgattatcaa attgatgcag aaactggaaa
    28501 aatcattgaa cgttctagag atcatatgaa tgattaattt aaataagata taaaaaaacg
    28561 aaggtctaaa aggccttcgt tttaattact cagtagcttg ctcccaacgt tttgaaagtt
    28621 tacgtgaaaa acttgagatg ataaagtttg taatgaaata gacagcagtt ataatagcgt
    28681 agagtgtaaa aacttgtcca gcttcgaaat atcttcccat tagaatttga ctttttccga
    28741 agatttcttg aatagctata acagaataaa gtagtgatgt atcttttata accgttacaa
    28801 actgagaaat aatggctggc aacattttgc gaaaagcttg tggaaaaata ataataatga
    28861 aaacttgcaa ataagtgaat ccttgagaaa gtccagcttc tgtctgtcct ttatcaactc
    28921 catttaaacc tccccgaata atttctgcta aagcagcgga ggtaaaaatt gtaaacgaag
    28981 taataccagc gctgactgat ttcatctgga atactaagaa aatgataaaa atccaaagta
    29041 gatttggaac atttctgaca aattcgatat agatactagc tattaatttc cagataggat
    29101 ttttgctatt tctcataatt gctaatagag ttccaaaaaa cattgatagt aaaatggaaa
    29161 taaaagatat atataaggtt aaaccgaacc cttgaaggag aaatagtata tttgttagtg
    29221 ttaagactga tgacatatca aacctcctta agaataagtt ttcttattta attcttcttg
    29281 ttttcttgcc cagcttgcta gtgggaaaca aataacaaaa tagaagatag cagctcctgc
    29341 aaaagctggg atataattag tcgtatcata agcccaggcc ttagctaaaa acatgatatc
    29401 ggctccagaa atgattgcta cggtagaggt attcttgatt aggttaacaa cttggttggt
    29461 cattggtggt aaaattgttc tgatagcttg aggcaaaatg ataagttgca tggtttgatt
    29521 agctgtgaat ccttgagcta aagcagcctc tgtttgtcct ttagggacag cttcaattcc
    29581 tgagcgaata acttcagaaa tataggcacc atggtatagg ccaacgcaca aaacagcggt
    29641 gaaaaaagca ctaatcataa tatgaccatt actaatgatt gctagtccat agtagacaaa
    29701 aaccatttga accaaaagag gtgtgttttg aaaaacttca acatagatac gtgatatcaa
    29761 cttaccaact ttacttttag aagttgataa accaccagag agaattccta atatcagagc
    29821 aagtaggagt gcaccaaacg aaatacctag agtatataag aagcctttaa agaataggtc
    29881 aaaatggtta aagaaagcgc cccaccttga aatggcaaaa ggactgatgt ttaataaagt
    29941 cataaaaaat cctccctatt ctttttttac tttagcgggt ttaagcttaa atttttgata
    30001 aatgtgtttt aaactaccat ctttttccca tgataccagt aacttattaa cgtagggaat
    30061 caagactttg tcctgtttac tggttgcaat accgtattct tgcgtattga acccttcttt
    30121 taatatggtt gtatgagggc taaggtagcc gctaaggata gatttatcaa ctgaaaaagc
    30181 atcaatacga ttagcgtaca gcgatatcgc taattctggg aaactcccta attgagcata
    30241 gctaaatttt agcttgtgtt ctttagcata tttttctaaa ttgacttttg tagtggcacc
    30301 ttgagccaca ccaatgtgtt taccatccaa ttcttttata gttttgatat gactattttt
    30361 acgcaccaaa aatccaattt catcgtgata ataagcttta gaaatattat agttagcttt
    30421 tctttcgggc gtgatagtgt atgttgcgat tagaatatca atttgcccat tgtccatcag
    30481 aggctctctg gtttgtgcag ttgtcggtac aaaaactggt ttgacgccta aggatttggc
    30541 aatttttttg gcaatatcaa tttccattcc ttcgtattga ttcgtctcag cattataata
    30601 accaaagtta ggcacatctt gcttgactcc aactttaagg acacccgatt ttttgatttc
    30661 tgataattga ttagctgacg ttgagttggg ataaaataac aggaaaatga aaatacagga
    30721 taatagatag agcgttaatc tttttcgtct cagcatattt cgtcctccta aaggtcagta
    30781 ctggtatggt tgataatgtt tcttaaaaat tgtttggcac gtggttctgt gggattatca
    30841 aaaaagtctt gcacatccgt agtatctact aaaatttccc cgtcagccat aaaaataata
    30901 cgatcagcaa cttctcgtgc gaatcccatt tcatgagtta caacgaccat attcattccg
    30961 tcatttgcca atttttgcat cactgaaaga acgtctccga tggtttcggg atcaagggca
    31021 gaggtaggct catcaaagag gaggagtttt ggatgcatgg caagccctct agcaatagca
    31081 attctttgtt tttgaccacc tgatagcata gatgggtagg aatcttttct ttcccacata
    31141 ttgacaaatt ttaaatattt ttcagcaatt tccatagctt cttttttaga ttgttttaat
    31201 actttaatag gagctaatgt aatattttct aacacggttt tgtggggata aaggttaaaa
    31261 tgttgaaaaa ccataccaac ttctttacgg aggttgacca attctttaga tgaaatattt
    31321 gctaattcat gtccattcac gaccaaacta ccatcgtcaa tagattcgag agcgttcatt
    31381 gttctaatga gtgttgattt acctgatcca gaaggtccta atagaacaac cacttgccct
    31441 ggctctattt ccaaattaat ctgacgaagg gcgtgataat ctccataata cttgttaaca
    31501 ttcttataag atatgagtga cataagaacc tcctaaaata acagtttatt gttaggttac
    31561 ctaacattat agataagtgt atcacctgtt tgtgagcttg tcaataaatt cagaatatta
    31621 aagagtagga ttgatttaaa cattgctatt tttagagcgg tatttctagt aaaaatatta
    31681 cgttctcgta tcatttattt ttcagtttaa taggatgtat tgaaagtttc caattaacta
    31741 ggaaatctgt tataattagt gataatagtg gtgctttagg cccatattat catattttcg
    31801 gtcactttta tttttcagtt tatggatgga agtgggagac cagaaagtaa tagaggtaaa
    31861 atatgaaaaa aatacttatc gttgatgatg aaaaaccaat ttcagatatt atcaaattta
    31921 atttaaccaa agaaggatat gaaacggcga cagctttcga tggccgtgaa gctttagtac
    31981 aatatgcaga atttcaacca gatttaatca ttttggattt aatgctccct gaactagatg
    32041 gtttagaagt ggctaaggaa gttcgtaaaa ccagccatat tcctattatt atgctttccg
    32101 caaaagacag tgagtttgac aaagtcattg gtttagaaat cggtgcagat gattatgtta
    32161 ctaaaccttt ttcaaatcgt gaacttttgg cacgggtcaa agcacattta cgacgtactg
    32221 aaaatattga aacagctgta gcagaagaaa gtgctcaaaa tgcttcttca gatattacta
    32281 ttggtgagtt gcaaatttta ccagacgcct ttattgctaa gaaacgtggc gaagaaattg
    32341 aattaacgca ccgagaattt gaattacttc accatttagc gacacatata ggacaagtca
    32401 tgacacgcga acatttattg gaaacggttt ggggatatga ttattttgga gatgtccgta
    32461 cagttgatgt gacggtgaga cgtttacgtg aaaagattga agatactcct ggtcgtccag
    32521 aatatatttt gactcgacgt ggtgtaggtt attacatgaa gtcttatgaa taatagtgct
    32581 gcaaatatta ggtcgtttga gctagcctta ctttttttac tagtttttgt tgctgtctat
    32641 tttgtttatc tagcggttag agattttaaa atgtctaaag atatccggct tttgaattgg
    32701 aaagtcagag atttgatagc aggaaattat tcagatagta ttctaattca aggtgatgca
    32761 gatttggtag aattagggga aagtctaaat gatttatctg atgtcttccg aatggcacat
    32821 gataacttag aacaagaaaa gaatcgctta gcgagtatct taacttatat gactgacggt
    32881 gtgctggcta cagatcgttc aggaaaaatc gttatgatta atgagacagc ccaacagcag
    32941 ttcaatctag catatgatga agctttatca atgaatattg ttgatatgtt agggtcggga
    33001 agtccatatt cgttccaaga tttagtctct aagacgccag aggtagttct taatagacgt
    33061 gatgaaaatg gtgaatttgt taccttacgg attcgatttg ctttgaaccg tcgtgaaagt
    33121 ggttttattt ccggtttagt tgcagtatca cacgatgcta ctgagcaaga gaaagaagag
    33181 cgtgaaagaa gactttttgt atctaatgtt tctcatgaac ttcgtacacc tttgacatct
    33241 gtaaaatctt atttagaagc tttagatgag ggagcgctca atgaagaagt tgctccaagc
    33301 ttcattaagg tatctctaga tgaaacaaat cgtatgatgc gtatgatatc agatttatta
    33361 agtttatcgc gcattgataa tgaagtaacg catttagatg ttgaaatgac gaattttaca
    33421 gctttcatga cctcaatttt gaatcgattt gatcagatta gaaatcaaaa aacagtcaca
    33481 ggaaaagttt atgaaattgt cagagattat cctcttaagt caatttgggt ggaaattgat
    33541 acagataaga tgactcaagt gattgataac attttaaata atgcagtcaa gtattcacca
    33601 gatggtggta agattacagt taatctacgc acaactaaaa cgcagatgat tttatcaata
    33661 tcagaccaag gcttaggtat tcccaaaaaa gatttacctc tcatttttga tcgtttttat
    33721 cgtgttgata aggcgagaag tcgtcaacag ggtgggactg gacttggttt gtcaattgca
    33781 aaagaaattg ttaagcagca taagggattt atttgggcta agagtgagta tggtaaaggg
    33841 tctactttta caatcgtctt gccttatgat aaagatgctg taacttatga agaatgggag
    33901 gacgttgaag attaaaacat gtcagaaata ggatttaaat atagtatctt agcttcaggt
    33961 tcaactggaa attgttttta tattgagaca ccccaaaaac gtcttttaat tgacgctggt
    34021 ttaacaggca agaaagtgac tagccttctt gcagaaatta atcgtaaacc agaagattta
    34081 gatgctatac tagttaccca tgaacattct gaccacatta aaggtgttgg cgtattggct
    34141 aggaaatatc accttgatat ttatgccaat gagcagacgt ggaaagttat ggatgagcgt
    34201 aacatgttag gcaaagttga tgttagtcaa aagcatgttt ttggacgcgg taaaacctta
    34261 actttcgggg atttagatat tgagagcttt ggtgtaagtc atgatgctgt ggatccccaa
    34321 ttttaccgta tgatgaaaga tgataagagc tttgtgatgt tgacagatac aggatatgtt
    34381 agcgaccgaa tggcaagctt gattgagaat gcagatggtt atttgatcga gtcaaatcac
    34441 gacattgaga ttctacgttc gggttcttat ccttggacac ttaagcaacg tattttatca
    34501 gataaaggac atttatctaa tgaagatggt tcagaaacca tgattcgcac tataggtaac
    34561 cgtactaaac acatctattt aggacacttg agtaaggaaa ataatattaa ggaacttgct
    34621 cacatgacta tggaaaacaa tttaatgaga gctgactttg gtgtgggcac agatttttct
    34681 gttcacgata cttcacctga ttcagctacc ccactgacga ggatttaaga ttgaaaaaga
    34741 ttttttacat tagtttagga tttattagtt tagggatagg cattgcaggt attgtcttgc
    34801 cagtagttcc aacgactcca ttagtattgc tttcagcttt ttgcttttcg agaagctctg
    34861 aaaaatttga tatttggttg cgtcaaacta aagtttataa atactacgct gcagattttg
    34921 ttgaaagcag aagtattgcc ccagcgcgta aaaagtcaat gatttggcaa atttatatct
    34981 tgatggggat ttcaatatat tttgctcctc taatgtggct caaactggga ttattgattg
    35041 gaactattgt aggaacttat gtgctttttt atgtagtacc tgataaaatt gatgaatagt
    35101 taacaagaga ctaggtgtgt atcctagtct tttgttgaat attcaaatat gtaattaagg
    35161 aaacgttttc tttggaagat aagtttaact tccaaaatgt ttgattattt gtcaactgtg
    35221 gtataatggt atttgtttaa tattttatct atgattgaaa tcaaggagaa aaaatgaaag
    35281 aattaagatc aaagctggaa aaagactatg gtattgtttt tgctaatcaa gaattattag
    35341 atacagcttt tacccataca tcatacgcaa atgagcatcg cctcctaaac atttcacata
    35401 atgaacgttt ggaattttta ggagacgctg ttctacaatt acttatttca cagtatcttt
    35461 ttacaaagta ccctcaaaag gcagaaggtg atttatcaaa attacgttca atgattgttc
    35521 gtgaggaaag tcttgcaggt ttttcacgtc tatgtggctt tgatcactat attaaacttg
    35581 gaaaaggaga agaaaaatct ggtggacgta atcgtgacac tattttagga gatttatttg
    35641 aggctttttt aggagcttta ttattggata aaggtgttga agtggttcat gcctttgtta
    35701 acaaggtaat gatccctcac gttgaaaaag ggacctatga gcgtgttaaa gattacaaga
    35761 caagcttgca agaattactt caatctcatg gggatgtaaa aatagattat caagttacta
    35821 atgaaagtgg tccagcacat gcaaaggaat ttgaggtgac tgtatctgtc aaccaagaaa
    35881 atttgagcca aggaattgga cgatcaaaga aagctgctga acaagacgct gccaaaaatg
    35941 ctttagcaac gttacaatga ggaaatcatg tttttaaaag aaattgaaat gcaaggcttt
    36001 aagtcctttg cggataaaac taaggtagaa tttgatcaag gagttactgc tgttgtaggt
    36061 ccaaatggat caggtaaaag taatatcaca gagagtttac gttgggcatt gggagaatca
    36121 agcgcaaaaa gtttacgagg aggtaagatg ccagacgtca tatttgctgg tacagaaaat
    36181 cgtaagcccc ttaattatgc gcaagtgagt gttactttag ataacagcga ccactttatc
    36241 gaaaatatag ctgacgaagt tcgagttgaa cgtcgtattt ttcgaaacgg agatagtgaa
    36301 tatttaatag atggacgcaa agttcgctta cgtgatattc atgatttatt tatggatact
    36361 ggattaggga gagattcttt ttctattatt tcccaaggac gcgttgaagc tatttttaat
    36421 agtaaaccag aagagcgccg tgccatattt gaagaagccg ctggtgttct gaaatacaaa
    36481 acacgtaaga aagaaacaca atccaaatta gaacaaactc aagggaactt agatcgctta
    36541 gaggatatta tttatgaatt ggacatgcaa gtccaaccat tagaaaaaca agcaagtatt
    36601 gctaagcgtt ttttagtatt agatgaggaa cgacaaggac tccacttatc tattttgatt
    36661 gaagatattt tacaacatca atcagattta actactgttg aggaaaagct attgacagta
    36721 agagaagagt tagcaactta ttaccaacaa cgtcagtccc ttgaagatga aaatcaatcg
    36781 ttaaaacaga aacgtcacca tttatctgaa gaaattgagg ttaaacagtt agctctttta
    36841 gacgttacaa agttaaagtc agatttggaa cgccaaattg atctgattcg tttagagtca
    36901 agccaaaagg ctgaaaagaa agaagaggca ggtcaacgct tagcagagtt agaagttaaa
    36961 gctaaagatt gtaatgacca gattacccaa aaaaatatag agttaactac tctgtcggaa
    37021 aaaatagccc aaataagatc agaaattgtt agtatagaaa gttccttaga gcgtttttca
    37081 actaatccgg atcaggttat tgaaaaactt cgagaagact ttgttacctt gatgcaagaa
    37141 gaagccgaca cttcaaacgc tttaacagct ttactagctg atatagagaa tcaaaaacaa
    37201 gcttctcaag ctaaatctca agaaatacaa gaagttagca aaaatttaga agtattaaaa
    37261 agtaacgcaa aggtagcatt ggaacgattt gaggctgcaa agaagaatgt tcgtcagtta
    37321 ttatctcact accaagattt aggtcaaacc ttgcaaaatc tagaaggtga gtataagaat
    37381 cagcagtcaa ttttatttga tcatttagat gaaatcaaat caaagcaagc acgtatttcg
    37441 agtttagaat ctattttgaa aaatcatagt aatttctatg ctggagtgaa gtcagttcta
    37501 caggctaagg accagcttgg aggtatcata ggtgctgtta gtgagcatct tagctttgac
    37561 aaacattacc aaacagcatt ggagatagct ttgggaggta gcagccaaca catcattgtt
    37621 gaagacgaga gtgctgccaa acgttcaatt gcttttttaa agaaaaaccg ccaaggtaga
    37681 gcgacatttc tgcctttaac tacaataaaa ccaagagaat tagcacaaca ttatctctca
    37741 aaattacaaa gttctcaagg gtttttagga atagctagtg aattggtaac ctatgatcaa
    37801 cgcttgtcaa acatttttaa aaataactta gggttaactg ctatttttga tacggttgat
    37861 aatgctaatg tagctgcaag acaattgaat tatcaagtac gtttagtaac cttagatgga
    37921 acagagttac gccctggagg gtcgtactca gggggagcaa atcgtcaaaa taatactgtt
    37981 tttatcaagc ctgaattgga taatctaaaa aaagaattga aacaggcaca aagtaaacag
    38041 ttaattcaag aaaaagaagt tgccgcattg ttagagcaac tcaaagagaa acaagaaacc
    38101 ttagcacaac ttaagaatga tggagaacaa gctcgtcttg aggaacaaag agctgatata
    38161 gagtatcaac aactatcaga aaagcttgct gatttaaata aactatataa tggcttgcaa
    38221 ttaagcagcg gggcgttgga acaaactact tctgaaaatg aaaaaaatcg tttagaaaaa
    38281 gaacttgaac aatttgctat taaaaaagaa gagttgacaa cttcaattgc tcaaattaag
    38341 gaagataaag attcaattca agaaaaagtc aataatctaa caacattatt gtcagaggca
    38401 cagctagaag aacgtgattt gctaaacgag cagaaatttg aacgtgccaa ctgtacacgt
    38461 ctagagatta ccttgtctga aataaaacgt gacattagta atttacaaac gttactaagc
    38521 catcaggaca gtcaattaga caaagaagag cttccacgta tagaaaaaca gttgctacaa
    38581 gcaaataaga gaagagaaaa tgacgaagaa aagcttgtga gtctccgttt tgaattggag
    38641 gattgtgaag ctgctttaga tgatttagct gcttctctgg ctaaagaagg acaaaaaaat
    38701 gaaagcctta ttcgtcagca agctcaatta gagtcacaat gtgaacaatt atctcaacaa
    38761 ttgatgatat tctcacgtca attatcagaa gactatcaga tgacattgga tgaagcgaag
    38821 gtaaaagcaa atgtcttgga agatattttg atggcgcgtg aacaattgaa atctctacaa
    38881 gctaaaatta aagctttagg acctgttaat attgatgcaa ttgctcaatt tgaagaagtt
    38941 catgaacgat tgaccttcct taacacacag cgagacgact tagttcatgc caaaaattta
    39001 ttactagaga cgattacaga tatggatgat gaagttaaga ctcgttttaa atcgacattt
    39061 gaagccattc gtcatagttt caaagaaacc tttgtacaga tgtttggtgg aggttctgcg
    39121 aaccttattc tgacagaagg tgatttatta tcagctgggg ttgatatttc ggttcaacct
    39181 cctggtaaaa aaatccagtc tttaaacttg atgtcaggtg gtgaaaaagc attatcagcg
    39241 ttagcattat tatttgccat tatccgtgtt aagacaattc catttgtcat cttggatgaa
    39301 gttgaggccg ctttagatga agcaaatgtt aagcgatttg gcgattatct taatcgattt
    39361 gataaatcaa gtcaatttat cgttgttact caccgaaaag ggactatgtc tgctgctgat
    39421 agtatctatg gggtaacgat gcaggaatca ggcgtttcca aaattgtttc tgtaaaatta
    39481 aaagaagctc aagaaatgac caattagaaa acagaagggg atcccttctg ttttttggat
    39541 tttcagcctt attttatgtt acaatagtaa atgtaaaccc tttagttagg agattccatg
    39601 ataaaattag tagcaaccga tatggacggt acattcttag atgaaaacgg tacctatgat
    39661 aaaaaacgtt tagcaaatgt gctaaaaaaa tttaaagaac aaggaattgt ctttacggca
    39721 gcaagtgggc gttccttatt atcacttgaa caattatttg ctgactttcg tgatcagatg
    39781 gcttttatag cagaaaacgg aagtgcagca gtcttattta atcgcttggc atatgaacag
    39841 cacttgagtc gagaacaata cttagatatt attgaccatt tgagtaagag cccttatatg
    39901 gagaataatg aatatgtctt atctggcaag gatggtgcat atattctaag tgatgccaat
    39961 ccagattata ttgaattcat cactcattat tatgataatt tgcaaaaagt ttctcacttt
    40021 gaagatgttg atgatattgt tttcaaagta acggctaact ttactgaaga gactgttaga
    40081 caagctgagg agtgggtaaa tcaagctata ccgtatgcca cagcagtgac aacaggtttt
    40141 aaatccattg atattattct aagctcagtt aataaacgaa atggtttaga gcacctctgt
    40201 gaacaatacg gtattagagc agaagaagtc ttatcttttg gtgacaatat taatgattta
    40261 gaaatgctag agtggtcagg aaaagctatc gcaacagaaa atgctagacc agaagttaaa
    40321 gagatagcag attgtattat aggtcatcac aatgatcaag ctgtaatggc ttatttagaa
    40381 agtatggtgg attaatgttg gatattaaaa tacttgcctt agatttagat ggcactttat
    40441 ttacaacaga taagaaagtt tcagaagaaa ataaagtggc cttaaaagca gctagagaaa
    40501 aaggtataaa agttgttatt acaacagggc gtcctttaaa agcaattggt aatttgttag
    40561 aggacctaga gttagtatct gatgaagatt acagcattac ttttaacggt ggtttagttc
    40621 aacaaaatac agggaaaatc ttagctaaaa ctgcaatgac acgacaagaa gtggaagata
    40681 tccatgagga gttgtatcaa gtgggtttgc caacagatat cttaagtgag ggaacggtat
    40741 atagtattgc taacaaggga caccattctc aatatcattt agccaaccca ctcttagaat
    40801 ttatagaagt tgatgattta gagcaagtgc caaaggatgt agtttataat aaaattgtta
    40861 gtgttattga tgctacatat cttgatcaac aaattgctaa acttcctgat agacttaaag
    40921 ttgactatga aatgtttaaa tcacgtgata tcattttaga attgatgcct aaaggtgttc
    40981 ataaagcagt cggcttagaa ctactgacaa aacaccttgg gttagatagt agtcaagtta
    41041 tggcaatggg agatgaagca aatgatttat caatgctaga gtgggcaggc ttaggagttg
    41101 caatggctaa tggtattcca gaagctaagg caacagcaaa agcgacaact atttgtaata
    41161 acgatgagtc tggagttgcc gaagcagttg ggaaatatat tttaagtgag gaaaattaat
    41221 gggattattt gaccgcctat ttggacataa gaaaaaagat aaagaactag aaattgaagc
    41281 atcagaatct gttgttttag aaaatgaaga ttcagttatt gataaagagg aaagcagcaa
    41341 cttttcgaag gagtcaactt tgaatcgaac ctctgaagtc cctgtcgcag aagatgattc
    41401 tttcttagaa ttagaaagag atacagcatt gtcagaatca catcaaccag ttacctctga
    41461 aattcatcct ttagagtcag aagatacctc tgaaattcct gtaaaagaag acgatagttt
    41521 tttagaacta gaagataggg ctaagacaaa ggtggcagat acttctgagg taggaaatgt
    41581 agttcctgat tccacgactc tttctgacaa tgtttctgct aaatctgagg catcttttag
    41641 tgatgaggag caatcaagtg atagtcaggc ttcagagcag ttttcagaaa ctccccttca
    41701 agaagagatg tctagtggaa aaactgaggt acaaacagag tcagaggata catctgcggc
    41761 tgatgctttt ttagcagatt actatgctaa acgtaaagca attgagaaag aaatctcgtc
    41821 taattcgctg tcaacagatg agtcagaact ttctgaggca caggaagttt tatcgcaatc
    41881 acaggcagac actataaaag ctgagagtca agaggaaaaa tataatcgta gcctgaaaaa
    41941 aacacgcaca ggttttagtg cacgccttaa tgctttcctt tctaacttcc gtcgtgttga
    42001 cgaagagttc tttgaagaat tagaagaaat gttaatctta tctgacgttg gggtaaatgt
    42061 tgcaacacag ttaactgaag accttcgtta tgaagcaaaa ttagagaatg ccaagaaatc
    42121 agaggattta aaacgtgtta tcgttgaaaa attagttgaa atctacgaaa aagatggtat
    42181 ttataatgag gctatcaatt tccaagaagg tttaaccgta atgctttttg taggtgttaa
    42241 tggtgttgga aaaacgacat ctataggtaa gttagctcat caatacaagt ctcaagggaa
    42301 aaaagttatg ttagtagcag ctgatacttt ccgtgcaggt gctgtggctc aactagtaga
    42361 atggggacgc cgtgttgatg taccagttgt cactggagaa aaaaaagcag atccggctag
    42421 cgttgtcttt gatggcatgg aaaaagccgt tgctcaaggc gttgatgtct tgttaattga
    42481 tacagcagga cgtttgcaaa ataaagaaaa ccttatggca gagttggaaa aaattggacg
    42541 tattattaag cgtgtcgtac cagatgctcc ccacgaaact ttgcttgctt tagatgcatc
    42601 tacaggacaa aatgctttaa gtcaagctaa agagttctcg aaaattacac cattaactgg
    42661 ccttattctg actaaaattg atggtactgc taagggtggt gtggtactgg ctattcgtca
    42721 agaactagat attcctgtta aattcattgg tttcggcgaa aaaatcgatg atattggtga
    42781 atttaattca gaagatttca tgcgtggcct tctggaagga attttataag aaaaaagaac
    42841 tttcatttag aaagttcttt ttatagtacg acgggcatgt cgtacatctg aggtgcaagt
    42901 cctctgtggg cacccgctac cggtgaaccc aatagcgact cccaagcttg actatcgtga
    42961 ggtagcgggg agaggaaggg atagcgaaat catggctcta
//
LOCUS       DATROY010000006        41845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.006, whole genome shotgun sequence.
ACCESSION   DATROY010000006 DATROY010000000
VERSION     DATROY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(76..1095)
                     /locus_tag="VBD11_000265"
     CDS             complement(76..1095)
                     /locus_tag="VBD11_000265"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6953245.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(1122..2000)
                     /locus_tag="VBD11_000266"
     CDS             complement(1122..2000)
                     /locus_tag="VBD11_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6953246.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(1969..2787)
                     /locus_tag="VBD11_000267"
     CDS             complement(1969..2787)
                     /locus_tag="VBD11_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6953247.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(2780..3112)
                     /locus_tag="VBD11_000268"
     CDS             complement(2780..3112)
                     /locus_tag="VBD11_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6953248.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(3141..4127)
                     /locus_tag="VBD11_000269"
     CDS             complement(3141..4127)
                     /locus_tag="VBD11_000269"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6953249.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(4124..5323)
                     /locus_tag="VBD11_000270"
     CDS             complement(4124..5323)
                     /locus_tag="VBD11_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6953250.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(5316..6152)
                     /locus_tag="VBD11_000271"
     CDS             complement(5316..6152)
                     /locus_tag="VBD11_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6953251.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            6291..6989
                     /gene="coaB"
                     /locus_tag="VBD11_000272"
     CDS             6291..6989
                     /gene="coaB"
                     /locus_tag="VBD11_000272"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6953252.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            6986..7528
                     /gene="coaC"
                     /locus_tag="VBD11_000273"
     CDS             6986..7528
                     /gene="coaC"
                     /locus_tag="VBD11_000273"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6953253.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            7574..8146
                     /locus_tag="VBD11_000274"
     CDS             7574..8146
                     /locus_tag="VBD11_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6953254.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            8256..9974
                     /locus_tag="VBD11_000275"
     CDS             8256..9974
                     /locus_tag="VBD11_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6953255.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKVKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(10038..10235)
                     /locus_tag="VBD11_000276"
     CDS             complement(10038..10235)
                     /locus_tag="VBD11_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953256.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(10249..11982)
                     /locus_tag="VBD11_000277"
     CDS             complement(10249..11982)
                     /locus_tag="VBD11_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953257.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLSLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(11983..13704)
                     /locus_tag="VBD11_000278"
     CDS             complement(11983..13704)
                     /locus_tag="VBD11_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953258.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSFGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(13716..14318)
                     /locus_tag="VBD11_000279"
     CDS             complement(13716..14318)
                     /locus_tag="VBD11_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6953259.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(14320..15297)
                     /locus_tag="VBD11_000280"
     CDS             complement(14320..15297)
                     /locus_tag="VBD11_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6953260.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEHFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(15302..16558)
                     /locus_tag="VBD11_000281"
     CDS             complement(15302..16558)
                     /locus_tag="VBD11_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6953261.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(16650..17246)
                     /locus_tag="VBD11_000282"
     CDS             complement(16650..17246)
                     /locus_tag="VBD11_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6953262.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(17239..18069)
                     /gene="prmC"
                     /locus_tag="VBD11_000283"
     CDS             complement(17239..18069)
                     /gene="prmC"
                     /locus_tag="VBD11_000283"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6953263.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKIEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(18069..19148)
                     /gene="prfA"
                     /locus_tag="VBD11_000284"
     CDS             complement(18069..19148)
                     /gene="prfA"
                     /locus_tag="VBD11_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6953264.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(19183..19752)
                     /locus_tag="VBD11_000285"
     CDS             complement(19183..19752)
                     /locus_tag="VBD11_000285"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6953265.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            19890..20072
                     /locus_tag="VBD11_000286"
     CDS             19890..20072
                     /locus_tag="VBD11_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6953266.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            20221..21159
                     /locus_tag="VBD11_000287"
     CDS             20221..21159
                     /locus_tag="VBD11_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6953267.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            21177..21779
                     /locus_tag="VBD11_000288"
     CDS             21177..21779
                     /locus_tag="VBD11_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6953268.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            21803..23038
                     /locus_tag="VBD11_000289"
     CDS             21803..23038
                     /locus_tag="VBD11_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6953269.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            23137..23925
                     /locus_tag="VBD11_000290"
     CDS             23137..23925
                     /locus_tag="VBD11_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6953270.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(24024..25298)
                     /locus_tag="VBD11_000291"
     CDS             complement(24024..25298)
                     /locus_tag="VBD11_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6953271.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(25298..25879)
                     /locus_tag="VBD11_000292"
     CDS             complement(25298..25879)
                     /locus_tag="VBD11_000292"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6953272.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAEAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(25983..26078)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(26134..27117)
                     /gene="guaC"
                     /locus_tag="VBD11_000293"
     CDS             complement(26134..27117)
                     /gene="guaC"
                     /locus_tag="VBD11_000293"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6953273.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(27321..28661)
                     /locus_tag="VBD11_000294"
     CDS             complement(27321..28661)
                     /locus_tag="VBD11_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6953274.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            28953..29624
                     /locus_tag="VBD11_000295"
     CDS             28953..29624
                     /locus_tag="VBD11_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953275.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(29695..31695)
                     /locus_tag="VBD11_000296"
     CDS             complement(29695..31695)
                     /locus_tag="VBD11_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6953276.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(31830..32591)
                     /locus_tag="VBD11_000297"
                     /pseudo
     CDS             complement(31830..32591)
                     /locus_tag="VBD11_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(32653..33645)
                     /gene="pta"
                     /locus_tag="VBD11_000298"
     CDS             complement(32653..33645)
                     /gene="pta"
                     /locus_tag="VBD11_000298"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6953277.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(33671..34555)
                     /locus_tag="VBD11_000299"
     CDS             complement(33671..34555)
                     /locus_tag="VBD11_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6953278.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(34552..35388)
                     /locus_tag="VBD11_000300"
     CDS             complement(34552..35388)
                     /locus_tag="VBD11_000300"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6953279.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(35363..36034)
                     /locus_tag="VBD11_000301"
     CDS             complement(35363..36034)
                     /locus_tag="VBD11_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6953280.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            36144..36716
                     /locus_tag="VBD11_000302"
     CDS             36144..36716
                     /locus_tag="VBD11_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6953281.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            36893..37867
                     /locus_tag="VBD11_000303"
     CDS             36893..37867
                     /locus_tag="VBD11_000303"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6953282.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            37871..38986
                     /locus_tag="VBD11_000304"
     CDS             37871..38986
                     /locus_tag="VBD11_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6953283.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            38988..39335
                     /locus_tag="VBD11_000305"
     CDS             38988..39335
                     /locus_tag="VBD11_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6953284.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            39490..40128
                     /locus_tag="VBD11_000306"
     CDS             39490..40128
                     /locus_tag="VBD11_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6953285.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(40141..40821)
                     /gene="radC"
                     /locus_tag="VBD11_000307"
     CDS             complement(40141..40821)
                     /gene="radC"
                     /locus_tag="VBD11_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6953286.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(40917..>41845)
                     /locus_tag="VBD11_000308"
     CDS             complement(40917..>41845)
                     /locus_tag="VBD11_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6953287.1"
                     /translation="SRLLMAIKRSVSMILSYATFIGLIVWLFSIVIPDLISSLSSLLV
                     IDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQVLSVLTNLLTSV
                     SSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFHYVRHILHQRFH
                     GFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGAYIGVTIGFILI
                     ATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAITIGGALWGILGM
                     LLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
BASE COUNT        13815 a         8020 c         6605 g        13405 t
ORIGIN      
        1 taaataataa aaatagtata gcaatattcc ctataaaaac atattttttt gcaaaaaaaa
       61 tttaaaatgt tcgtcttatg aaaataaaac tgcttttatt tcttctggac tagtctgacc
      121 aaaataatct tctaatgata aagtacttaa tttttccaag actaattctt ctttcatagg
      181 gcaaccaatg agaaggtttt caatatctga aatatcttta gaactgaaaa agtcaccata
      241 aatacggcaa ttcgtaataa tacctttctc aatttctaag tgaaaatcgt aagttcctga
      301 cggaaaacga gcatctctgt gatacgaata ctgtggtgag ttaccataat tccaatccca
      361 attcttatat ttttcatcag ctagagcgtc aatcgcagcc caatcttttt cggttagatg
      421 atagcgtttt gcctgatcaa tatgatcaat atgtaataat tgacaaacca ttaaatcttt
      481 aaattgatcg gttgtgatat gacgataatc ttctgacaag tggctacgaa tatctcctac
      541 tcgacttcgg acagatttta tccctttaga ttcaattttt ttccgattag gatttaacac
      601 tttttccata gcatcaaaat caacatctaa taaaagagag taaccaccgt aaatacgacc
      661 gtttactatc gtcattgctg cacctgatac ttttttacca tcaataacta agtcatttcg
      721 tcccctcatt tcaacactag aagcacctaa agtttttagg gccttaatag ctggctcata
      781 agctcgtttg aaatcaccaa attgtccatg atctttcatt agatagcaaa tatttacagc
      841 acctgagtca acgtaaacag ctcctccacc agtatctcga cgaacaacag gaatatcatg
      901 ttctttcaaa tattcttgat ttgtctctat aactgcattc tgaaattttc cgatttcaac
      961 tttaggatca caatagtatg gaaataaaat atcatcatct aaaaaaacat tttgtaaaat
     1021 ataaacttgc atggcaagtg caatcgcacc gtctgtgata cgcttaccat ttctaattgg
     1081 ttcaataaga tacatttagg tctcctttta tagcaattta gttatccaat aatttctttt
     1141 ttcgcttcag caattaattc agcgatatgc tgtgtcagtt gcacactcct tgagcgaatc
     1201 ttttgaggaa tgcgataatg cttgtgatta agagtaacaa ataaagagtt ttcatttaaa
     1261 gagacgaatc gccaaaacgg atgcttaata aattgtggcg tggtatgtcc aactccaatc
     1321 tctagataaa gaactttgtc attttgatgt tgtgataaga aattttcata acgttgtcgt
     1381 tgagcatgaa agtcagcatc ttccaccata cctttttctt cgttacgttt attaatttca
     1441 aacggttgat cacactttgg gcatttgggg attaagttac taggaatctt catatccttt
     1501 tgttgtgcaa tcatttgacg aattgcctga tcatttcgat aagtttgttg atgacaatgt
     1561 tgactacact gccataagcc atattcacct tgaatatgaa aaactttttc taaattataa
     1621 tcagccacag cgaaggcatt atcagcatta gttgtaataa tatgatattc ttttttcgct
     1681 aaaatatcct ttaaatcaag ataagcttgt cctaccggtt gatctaaata attaagtgcc
     1741 acaaaacgac tttggaaggc ccaatattct tcccaatctt caaagtcata aagacttgct
     1801 tgcaacatat ctaatagttg atatttagca ataaaatccg gaaaagcttc ttcaaatctg
     1861 gggccaatat aggtaaatcc atccgctgca gacatgcctg caccaatacc aactacaata
     1921 gcatctgatt cttctattaa ctgacttagt atttctgatt gagaatgatt agttttttct
     1981 agagtcttcc acactgtcat ctatttcctc ttttaataat aatttttgat aaatctcttg
     2041 gtctttaggt gtgaatgtat tgaaaataat atagagtttg tgctgatttt cgcgttgcca
     2101 ctctaatatc tcttgaacag ccaattcagc ggctctttga ttaggaaaac caaattctcc
     2161 cgttgatata caagggaaga caattgtttt aagattagct tgagctgcca attgtaagca
     2221 ggatttataa gaagacttta ataaatcctc tcttatacga gatggctttt gatcaacttt
     2281 tacataaggt ccgactgtat gaataacata cttacaagga agatgatagg attcggtaag
     2341 tttagcttgt cctacggctt ccattcgtcc ttgttgtgtc atcaattgat gacatgctaa
     2401 acgtaatcgt gaacctgcaa acgtatgaat ctgattgtca atacaatggt gattaggaat
     2461 aaaacaacct aacaacttac tatttgctgc attaacaata gcatccactt gtaataagca
     2521 aatatcccca tggtaggtaa atacatttga atataccgtt tgatgacaga cgtctatcgt
     2581 ttttactttt tgagtattcc accaatgtga caaataccta tcttctaaag acaggtactc
     2641 ttgactaatg ttttgaggat cttgttgatt aactagacca cgccaaaccg tttgcaaatc
     2701 ggtggtcctc aagtcatcaa cgtctgttaa tttttctgac tgaagatact caatcatggc
     2761 tagcaatagt tgtttttgat taggcatttt ctaaagcttc aaaagcagca taatcaacat
     2821 ctgttaacac aaccaaccaa ttttcgtcag ctttttcaga atttaacaaa gttggttctt
     2881 gacttgcagc tgtattaact ttaacaactt tacctgtaag aggagataaa attgccataa
     2941 ccgtctttga agcttcaata tttaaaataa catcatcaac ttctaaattt gtatcatctg
     3001 tgaattccac atatccaaca gttcccaagt catcttgaag ttcgggagtt aagctgatag
     3061 tataaagttc ttcatttttt tcaattaata agtagttcgc aatttttttc atgagtaatt
     3121 tcctttctga gaaaacgtga ttaatataac tgtgataata attttaagga tttgattcgt
     3181 tgatcaagtc ctggaacaag aggaatcaaa agtagttctt ctgcttgact agcattaact
     3241 aaagcgtcaa ggctttcttt aacttgtttt ggatcaccta caatcatacg gtggcgattt
     3301 gatttaattt ttgctttttg ttcaggagtc aattggtaat gattagcttc ttcaatagta
     3361 ggaaatgttg caaattcatt gaagtcttta tttcctagca tccagatatc taaagtcttt
     3421 gctatgtttt cagcttcttc tgaagtatca gctataacta caaatggagc taccatcaaa
     3481 ttaggagact tattaggata atattcttca aactgatgat aataaagaga tgaaagcttt
     3541 ttagcttctg tcaatgggtc tttgtccata aaaggaaaaa caccgaaggt aaagcctaag
     3601 ccaagtttag ccgctaaata agctgatttt tgaccactcc ccaacacata taagtctggg
     3661 aagctagaaa gagtcggttg aactaatggt tccttactgg atgattcatc aataagccat
     3721 gacttcaatt cctccagtac ctcttcgtaa tcatgtgctt tatgtaagct acgaagtgca
     3781 tttgaaactt taactgtccc tagtgaattt cctaaaccaa tacttactcg attcggatga
     3841 catgtctcta atgtcttgag agtctccgcg agtttaaaac tactatagtg cagaggcatt
     3901 atacctccag agccaatttt gatagattta gtctggttag ccaaatgcat tatcattaat
     3961 tctggattgc taatactgaa tgccttaaca ccgtgatgtt cagccaccca aaattgatga
     4021 tagcctagtt gctccgcctc ttgtgataaa agaatcgttt cttgtattgc atcacttgca
     4081 gtttttccat aatcaataag cccataatca aggacactta atttcatctt tcctccctat
     4141 aattgcttcg aagttctcta aaaacttctc gagcttcctt tggtaaagat tctccataat
     4201 ccatcaaagg aacaatatct ttaaaggagg cttttggaat cgccaaacct tctaaatcat
     4261 tcactttaat ccttaaatta atctgatcag aacgcttagc ttttattttt tgtaaaaatt
     4321 ccggatcaac tactaagggc gtggaagctc ctatcatatg agcatgttgt aaggcttcaa
     4381 acacctttga agcactatta atacctccag ttgccataat tggaactcta tcaccaaaat
     4441 gttcaaatat aacttgatta accagctgcc ctttgtaaac tccctcagaa cgtatagtat
     4501 ttctaaagac atcatgcccc cagcttgcaa tggcaagata atctacctga gcaatcgcta
     4561 aaattttttc aagaaattcc ataaactctt caatgctata tccaatttgt gaaccgcgag
     4621 tttcttcagg cgtcgctcta aaaccaagga taaaatctga ctccgcttct tctctgataa
     4681 ctttttgtac cgctttaaaa acttctaagc ctaaacgaca gcgattagtc aaagtttgag
     4741 gaccatactc atcttttctt tgatttgaga aggttgagaa aaaagtttga attagtaatc
     4801 gttgtgcaga agatatttca acaccatcaa atccagcttg tatagctcgt cttgtagcct
     4861 ggacatattc atcgataatc cttagtatat ctttgtgtgt tagttcttta acctgatgtg
     4921 ggtatggcga ttgcaattgc atagggctgg gaccatatac atatccgtgt cttgccaaag
     4981 tgtgacttga gaaacgtcca gcatgcgtca gctgcaaaac agctttcgct cccttagact
     5041 tcattgcttt agccaattta gtcaaaccag gaatgtcctc atcctttgaa acgctaaaac
     5101 catattcaaa taactgacca tattctgtga tatatgctgc tccagtaatt tgtaatggag
     5161 cggatttagc acgacgaaca gcatatgcta tgtcgtcatc agttacaaaa ccttctgatg
     5221 tagaagaatt agttaccata ggtgatagaa caaaacgatt ttctaagctc aatccattgg
     5281 gaagtgttaa tggacgaaat aaattacctt ggacattatt catctctgct caccatcccc
     5341 ttattagcca tagacttagc ctgaaatcta tcaaactctg caactaaatc gggtctaatc
     5401 atcagtaaac gatcattaaa acctacttgt ttaaggctaa tcaacatacg atcaataaca
     5461 tcttctactg tcataggaca atctaataga tcagatagat tagccataat ttcaggatct
     5521 acatttggat aagcaattgg actacccgta tcacctagac caatcttata aaaatctgaa
     5581 atcatttgac tccgcccttt ttgatcgcca caaacgctaa ggtaaattga taccgcaata
     5641 ccattcttta tacggcgctg agccaagcca gcaaattttt tgccatttat actaagatca
     5701 aacttaccag gacaatagga ggtctctact tcaaagtgct caataggttg ataaaaatcc
     5761 gaaaatatac ttctaataaa atcgaccatt ataagatacc catctgagat agacaatttt
     5821 ctctcaaaaa catctggaat aaccaatgaa aaatttaaaa ttccttcatc agctacaact
     5881 gcgagacctc caaaattccg aacaactggt tcataaccac gactgatgat ttctttttta
     5941 gctaattcta aatgtggtaa ttctcgatct aacatcccca gaatgactgt cttagtcatc
     6001 ggccaaatat gcaaaataat ttcttgattt gagcgattaa tctcccttaa aaatacttct
     6061 gtccatataa aaggtttttc cgcgtcttga gcatctgtaa catagtcttt aaaaatagat
     6121 acaggtaact gcgctagatc ttgccactcc aataaatctg acctcctttc tctagaaaac
     6181 tctgcaagat ttattttatc gcaatcgctt gaaattttcc agtaagtttc cctcaaattc
     6241 cattactatc aagaagaaat atatctttta tttacgaaaa gcattttttt atgtacaata
     6301 tggctatgaa aattttgatt acatcaggtg gaactaccga gaaaattgat accgttagga
     6361 gtatcacgaa tcacgcaaca ggagctttag gaaaaataat tgcagaaaaa tatttacgag
     6421 aaggacatca ggtgacactc gtgactacta aaaatgcagt caaaccagaa tctgctacca
     6481 acttatccat atttgagatt gaggacgttg actcattaat aaaaaccttg aagccattag
     6541 tgaaagagca tgatatttta attcatagca tggcagtgtc tgactatacc cccgtctaca
     6601 tggctgattt cgaaaaggtt aaatcaagcg atgatttaga tacttttttg cgcaaagata
     6661 accacgaagg gaaaatatca tctgaatcag aatatcaagt cctttttctc aaaaaaacac
     6721 caaaagttat cagccttgtg aaaaaatgga atcctcaaat tactctagta ggttttaaac
     6781 tacttgtgaa cgttactaaa gaaaaccttt ttaaagttgc tcgtcatagc ttaatcaaaa
     6841 ataaagctac gtttatttta gctaatgacc tcattgatat tacctcaaaa catcacatag
     6901 cctacctact tgatcatgat aatgtgtata aagctaccac caaagaagat attgctcaat
     6961 taatttacga gaaggtaaaa aaaatatgat taaacgtatt acactagccg tcacaggttc
     7021 aatttcggcc tataaagcag cagaccttac aagtcaatta actaaaattg gctatgatgt
     7081 tcatatcata atgacacaag ctgccacaca atttattaca ccgttaactc tgcaagtatt
     7141 atcaaaaaat cctattcatt tagatgttat ggatgagcat aacccaaaaa tcatcaacca
     7201 tatcgaatta gcaaaacgca cagatttatt tatcgttgca cctgcctctg ctaatactat
     7261 tgctcatcta gcatatggct tcgctgataa catcgtaaca agcgttgctc ttgctatgcc
     7321 cgatgaaact ccaaaactta tcgcaccagc aatgaataca aagatgtatc ataatacaat
     7381 cacccaacgt aacattgata ttttgaaaaa aataggttat caggaaattg aacctcgtat
     7441 tagtttgctt gcctgtggtg ataccggtca aggagcccta gctgatatct ctactatact
     7501 taaatgtata caagaagttg caaattaggc tgtaaatttg tatactattt ctagtttaat
     7561 ttaaggagtt ataatgaaaa agaaaaacaa atcctctaac attgctataa ttgcaatctt
     7621 ttttgctatt atgcttgtca ttcatttttt gtcatcattt atttttagtt tttggttagt
     7681 ccctattaaa cctactttga tgcatatccc agttattatt gcatctatag cctatggacc
     7741 tcgtattggt gcaactctag gcgccttaat gggggggatc agcgtagcta acagcagcat
     7801 tgttctatta ccaacgagtt acctcttctc accttttgtt gaaaatggta atttttattc
     7861 gctaattatt gcacttgtac cacgtattct aatcgggatt attccttatt tcgtttacaa
     7921 attactacac aaccgctttg gtttggctat ctcaggtgct ataggctctc taacaaacac
     7981 agtatttgtt ttatctggaa tttttatctt tttttcaagt acttataatg ggaatatcaa
     8041 gctaatgctc gctgggatta tttcatctaa ttcattagct gagatggtca ttgcagctat
     8101 cattgtatat ctaactgttc ctcgtattct caatattaaa cattaaaaac tggccaggaa
     8161 gaaatcttcc tggcttttct tacttatatt ttatagtcat attgaaaaaa acatgttata
     8221 atgaaagcgt ttataaatat tgaaggagta tttcaatgac ttacacagaa aattttcaaa
     8281 aatggttaga ttttgagcag cttcctgact atcttcgtca agaattacta tcaatggatg
     8341 aaaaaacaaa agaagatgct ttttacacaa acttagaatt tggtactgct ggtatgcgtg
     8401 gttatattgg tgctggtaca aatcgtatta atatttatgt tgtccgtcaa gcaaccgaag
     8461 gattggcaaa attaatcgaa actaagggag aggaagctaa aaaacgtggt gtagccattg
     8521 cttatgattc acgccacttc tctccagaat ttgcttttga gtctgcacag gtcttggcac
     8581 agcatggaat taaatcttac gtatttgaag ctcttcgtcc aacaccagaa ctctcttttg
     8641 ctgttcgcca tttaaatgcc tatgctggta ttatggttac tgcaagccac aatccagcac
     8701 ctttcaatgg ttataaagtc tatggacaag acggaggtca actaccacca gctgatgctg
     8761 atgcactaac agactttatc cgtgctattg aaaatccatt tgcagttgaa cttgctgatt
     8821 tagatgaaag caaatcatcg ggccttattc aggttatcgg tgaagatgtt gatattgaat
     8881 atcttagaga ggttaaagat gttaatatca atcaagattt aatcaataac tttggaaaag
     8941 acatgaaaat tgtctatact cctctccacg gaactggtga gatgttgaca cgccgtgccc
     9001 ttgctcaagc cggttttgaa tctgttgttg tagtagaatc acaagctaag gcagatccag
     9061 acttttctac tgttaaatcg cctaaccctg aaagccaagc tgcctttgct cttgctgaag
     9121 aactcggacg agaagttaat gcagatgtct tagtagctac tgatcctgat gcagaccgcc
     9181 taggcgttga aattcgccaa ccagatggtt cttataaaaa cctttctgga aatcaaattg
     9241 gagcgattat tgcgaaatac atcctggaag ctcataaaac agcaggaaca cttccagaaa
     9301 atgctgctct cgctaaatca atcgtatcaa cagaattagt aacaaaaatt gccgaaagtt
     9361 atggcgctac aatgtttaac gttttaactg gattcaaatt catcgctgag aaaattcaag
     9421 agtttgaaga aaaacataac cacacctata tgtttggttt tgaagagagc ttcggatatc
     9481 ttattaaacc atttgtccgt gataaagacg ctattcaagc agttcttttg gttgctgaga
     9541 ttgcagctta ttaccgttcg cgtggtttaa cacttgcaga tggtattgat gaaatttaca
     9601 aagaatatgg ttattttgct gaaaaaacga tttcagtaac actttcagga gtggatggag
     9661 ctgctgaaat taagaagatt atggacaaat tccgtgaaaa tggtccaaaa caatttaaca
     9721 atactgatat tgtattatta gaagatttcc aaaaacaaat cgctactaaa aatgatggaa
     9781 caatatctaa cttaacaact cctccaagta atgttttgaa gtacacgtta gctgatgatt
     9841 catggattgc agttcgccct tcaggaactg aaccaaaagt caagttctat attgctacgg
     9901 tcggtaatga tttagcagat gcagaaacaa aaatcgccaa tattgaaaaa gagattacaa
     9961 catttgtaaa ttaataaaaa aagctaggaa cttactccta gcttttttta tatctatcta
    10021 acgatgttct attattttca atcatgaaat ataactgcaa ttaacttcac gatcaaataa
    10081 tagagcagac ttacaattaa ccacgttatc ccaataactg caactaaacc tattaaaaca
    10141 gataataccg tattcttagt tgctagttgg ttactgataa accatgaaac tgcagaaata
    10201 aatatcagta ataatagttg aagccttttt ttcatataat tcctcttact acttttgtgc
    10261 attttgcatt tgataataaa tacctttttg agctattaat tcagagtggt tacccacttc
    10321 tacaatttca ccagatacca taactaaaat aatatctgct gtttgaatgg tagataagcg
    10381 atgtgcaata ataaagcttg ttctcccctt catcaacatt tggaatgctt cttgaacaag
    10441 aacttcagta cgagtatcta tagacgatgt cgcctcatct aatatcaata ttctaggtaa
    10501 tttaagaaaa atacgagcaa ttgtcaatag ctgacattgc ccctgtgata acgaatctcc
    10561 agcatcttct aaatacgtat catatccatt cggtaattgt ttgataaaga aatctgcatt
    10621 agctgctttt gcagcctcta tcacttcttc acggctagct ttaggattgg catatgcaat
    10681 gttatcgtga atcgtagcac tttttaacca cgtttcttgt aataccattc caatttcttg
    10741 ccttaattgt gatggctcat aatcagtaat aggcttacca tctaaaagga tatttccccc
    10801 gtcaacttca tagaaacgca ttaataaatt gattaaagta gacttcccag cacctgttgg
    10861 tcctacaata gcgaccttag ctcctgctgg aatgtgtaaa ttaataccat tcaatagtgg
    10921 ttttgattta ttataaccaa aaataacgtt tttaaaatcg atttgacctt tgaccgttga
    10981 tgaatcaagt ttttctgtac ctgttatatt tggtgacgat tcttctaaaa tagagtaaag
    11041 gcgttctgca caagccagag cactttgcat ctcagacagg acggaagaga tgtcattaaa
    11101 tggcttagta tattgattga cataatttaa gaaagtgatt aattgaccaa cactaaaagc
    11161 acctgacata atacgaaggg caccaatccc tgctaagaag ccataaatca aactattgat
    11221 aaatcgggtt gatggattga cagttgaaga ataaaaaatt gctgattgag aaaagttagc
    11281 atactcttga ttaatagtcc taaaatgatc actactttct tcttgcgcac tgaatgcttg
    11341 aataaggctt tcttgactca ccatctcttc aatgaattga gtttgtcgtc ctcgtgatgc
    11401 cgtttgattt tgatataagt gataactctt tttagcaata aagcgggcta gaaagagtga
    11461 taaaggcgtc agaaataata ccaaaaaaag cattaataaa tcaatctttg ccatactaaa
    11521 aatagtaact agaatagtta ataaaccaac aaagaactgg ttaaaaacca ttaataaacc
    11581 atttgataat tgctccgtat cggtagtcac acgactaatc aagtcaccta tgccacgttt
    11641 atcaaggtaa gctataggta atagactcaa cttttccatt acagcctttc ttaaagaagc
    11701 aacataatga aaaattaagc ggttatagag cagaggatta atccattgaa taatagtatt
    11761 tgctaaaata actgcaacca tcttccacat tataggtaac aataatatca tagaatgagg
    11821 cgataataca acgtctaccg cctgtccaat tagaacaggc aaataaacag tgaggcaaac
    11881 ttgtactacc gtccctaata atgccattcc tactaagctt tttttagaaa gaagatcttg
    11941 aacaagcttt ttttgtatct ttttaggatg acttgctttc atttactcct cctccttaaa
    12001 attttgtgaa tggtaaatgg acttataaac tttgttatga gctaataaag attgatgatt
    12061 atcaaaacca acttgtttgc ccttatcaag gagtaagatt ctatctgcat tttgtatact
    12121 attaattctc tgtgatacaa taataagatt agtttggttg aaatgtttaa ttatagcttt
    12181 aaataaacga gcttctgtta aataatccaa tgcagatgtt gcatcgtcta aaatcaaaaa
    12241 tggaatctta tcttgaacta aagctcttgc aatcgtcaac ctttgccttt gaccaccaga
    12301 gaaattccta ccaaaactct ctacaggggc atcaagttga ccatcttttt cttttacaaa
    12361 atcactagct tgtgctattt ctaaagcagt ccaaagtttt tcctcactaa ctttaccaag
    12421 tccaaaagaa aggttagaac gaatagttcc tttaaacaac tgagcatttt gaggaacaac
    12481 tcttaccagg ctacgccaat ttgaaattgt atctggactt tttccctgat gatatatatc
    12541 aatatcccct tcttgcactt tataaatatg aagcagtaaa ttaaccaagg ttgatttccc
    12601 agagccagtt ccaccaatta tccctaaagt ttctcctaca ttcatagaaa aagtaatatc
    12661 agataaagct ttttcagagg agtttggata agaaaatgcc atttcttgca cttctaaagc
    12721 cttattcgaa tattttggct caagtttgtc atcaattatt tcagacggtc tttcaaaaac
    12781 ggctataatt cgcttagcac tgatataact ttggttcagt gatgtcacca acattgtcat
    12841 ttttaacaat tcaacaagaa tttgcaataa gtaattaatt aaagcaacca acattccttg
    12901 tgataaaaga tgattagcta tatttaaatt accacgccaa ataatcacaa ccaaagtgat
    12961 attaacaact agaaatgtta gaggcgttac taaactagaa agcctacctg cttttaattg
    13021 taaattcgta tagtgataat tgatatcatt aaatgcttca ctctctctat ctacttgatt
    13081 aaaggcacgg ataactcgta caccttgaag ttgttgccta gtcaacttta ccaaataatc
    13141 ggttgaagtt ctgattttaa ggtaaatagg atttaatagt cgagacatta caaaaacaat
    13201 gataaataac gtgacaacca ttatcaagaa ccaaatagtc aatgagggac taatggaaaa
    13261 agccataatt atggcaccaa aaacaataat cggagccctc aaaaataaac gtaaaaattg
    13321 atttaaacca gtttggatgt gaaatgtatc agcagtcaaa cgagtaatca aactagcagt
    13381 tcccaattca tctctgtctt ttttacccag tgacatgact ttttgataga gatcctctgt
    13441 caattgtctc gtataaccaa ctgcggcttt tgaagaataa tattgagcag ttatcgctac
    13501 tacaacaccc accgcagcca aaaagaaaat caatccaatt tgcaacagta aactacttct
    13561 atcccctcgt ggaatggtct cgtcaatcat ttttgcaata acgattggta ccaaaagttc
    13621 aaaagaggct tctaataatt tgaaaagggg tccaaagata gtttctttga tatacccctt
    13681 aaaataataa gttagtcttt tcatacttac ccttcttaaa acaactccat tatttttatc
    13741 aaataaaggt tgaattgaac ttcacgtgag tagtaaatat taccaccatt tttatagagc
    13801 ttccctcctg aaattaaggc gagaggatga tagtaaaagt aagtgtcccc gttataattt
    13861 cctaggctgg gagctacaac atttttagag taatttttgg ccaactcaat ggaattttca
    13921 cccccatgat ccgctacata atcaatataa gaagaaccaa aattatatgc ttggacagca
    13981 gtccatacat caaccttagc ttttttagct tgacttatat tttgtgacaa cagagtgacg
    14041 ccatgtttaa tactggtgtg actatccgta atactgttcg tagttccact gatactttca
    14101 cttgattgca tcacatctat agcatctccc ttagtttcag tataaatcat agataatact
    14161 aagtgcacat tagcctctga accattctct gcaagagtat tctgcactaa atcatgatat
    14221 tgaagcactt ttttaacatt ttcatggatg ataactagac ggtacccaat gaaaataata
    14281 ataattaatg ctatcaaacg ttttaaaaat ttaaacatat tatttatttt gaatatcctg
    14341 tatgtttttg attagtattg agtatgtacc atcagggttt tggataaatt caactgattc
    14401 ggcatcttgg tagacattgt tgggtacaat caattcaata ccatttgaaa gagatagctt
    14461 ctgattctct aacttcttaa tttgacggga atgatcaata tcacttactt gaacagatcc
    14521 aggtattgcg tctttcaatt catcaacaaa agtcagcctg gctgttaaat tatcatcaaa
    14581 taattgatct gctaattttt ctggagacaa ttcttgctct tcttctaaat tcttataaat
    14641 agccgatttc atttttgatt ggaaagaaaa atcatctttg ttgaaatttt cagcaatttt
    14701 ttgagctgtc tgttcaacca tttttataga ttttttaaca gactgttctg gttgtacttg
    14761 taataaattt tcagaaaaat aattagcaaa tgaaccattg tgtttgatac gcttttcaat
    14821 taaatagtat tgctttgatg acttgttaac aaccaaagct tcatctggag tttgagcagc
    14881 acttggaagg ttattctgtg ttattgtaat tggctgctct tgattttcag aaacatgagc
    14941 aaaatgttcc tttaatgaga ttcgaaggaa agcaaaatgt tccattccat ccttgtcaaa
    15001 ttgaataaaa actaagtcat tcgttttttg atcttccgat atgacaaact cttctttcca
    15061 aagttgcgca atttgacagg aagaaacata taaatcatct tgtaaatgac tcataaatac
    15121 attatcctct ccaaaatagc cacgcttagc ttcatcagaa aaaactttac ttaatttttt
    15181 acgaaagtag tcatcaatcc ttggagttag ggttaacgga gtatctgata gcactaactc
    15241 tgtgtcgtta ggagaaaact gatgtataat aatctgtttg atataaaaat ccatcataac
    15301 attaattctc atacaaaggg aaagcgtccg ttaatgattt aatttcttga cgaacttctg
    15361 tgaggacatc ttgattttca tgatttttga gtgccttaat cattaattca gcgattcttc
    15421 gagattcttc aactcccatt cctcgactag taattgctgg agtaccaatt cgaatacctg
    15481 atgttttaaa tggtgatagt cgctcaaacg gtatagagtt cttgttaagg gtgatattga
    15541 cttcttcaag aacattttgt gcctttttgc cattctcaat aacttttgtg acgtctacta
    15601 agaacaaatg attatcagta ccatcagaaa tcaaatgaaa gtcatcatct tctttaaaaa
    15661 ctttagccat ggcttgagca tttttaataa tatcctcacc gtatatttta aaggaaggat
    15721 ctagagcttc tttcaaagct acagcttttg cagcaattac atgttctaat ggtccgcctt
    15781 ggagtcctgg aaagactgca gaattaattt ttttagcaat tgcttcatca ttggttaaaa
    15841 tcaaaccacc tctaggtcca cgtagagtct tatgtgttgt cgttgtcgtt acatgagcat
    15901 aaggaatagg actaggatga tgaccagaag caactaaacc tgcaatgtga gccatgtcaa
    15961 ccattaagta agcatctact gcgtctgcta tttgacgaaa cttttcaaaa tcaataatgc
    16021 gagaataggc cgacgcccct gcaacaatta atttaggttg cgtttcctgt gctattttca
    16081 aaatgttatc atagtctagc atctccgtct taggatcaac agagtaagaa acaaaatgat
    16141 aagtttttcc tgagaagcta actgaagcgc catgtgttaa gtgcccacct gctgctaagt
    16201 ccatccctaa gacagtatct cctggctcga ttaaagccat gtaagctgct gcattagcct
    16261 gactaccaga atgtggttga acattagcaa actcagcatt aaataacatc ttagctcgtt
    16321 ctatagctaa actttcaact acatcaacac aatctgttcc tccataataa cgatgactag
    16381 gatacccttc ggcatattta tttgtcaaaa cagaaccttg tgctgccata acagcttttg
    16441 aaacaacgtt ttctgatgca attaattcaa tattattttg ttggcgaatt tcctcatcat
    16501 gaatagcctg ccaaagttct tggtcaaatt ctttaaaatt gtctttatca aaaatcattt
    16561 tttactcctt aagctcgtta taacttttaa ataatggttt caatcattat atttatcaca
    16621 tttttactat tagttgcaag atgattaaat taattttcaa aaattaattc aggtatcgta
    16681 gccgttagta cctcttgcgt gattgcacct tgccttaaaa tcttaactct atcaccagac
    16741 aaatcaataa tagtagaatc cacacctgtc aatgctttat catcttcaat acctaaaact
    16801 tgatgattaa actgtttttg aatttctgag aatacacgac cactatcaca tccagaaata
    16861 ttagcagaag gaccaatcaa tggaccatgg ttagcaatca tatcaagggt tacagggtgc
    16921 ttaggcatcc gaaatcctac gcttgataac ccagaattga tccaatccgg aacttcttgt
    16981 gaagcttcca aaataatggt tagcggtcct ggtaaaaaag catcgtacaa ttgcttcaaa
    17041 taggtcggtt gatttttaga atattttaaa atagtctcaa aatcacatat atttaagttc
    17101 atagccttat cacgaggtct ttttttaagc tcatagacgt ggttcacagc ttcttcactc
    17161 aaagctttgg caaagattcc gtagaccgtt tctgttggca taataactgc gccatgatcc
    17221 tctaaaatct gtcccaaatc attcattatc taataccacc attctatctt taccaaatat
    17281 atctttcaaa actctgcaac gattgtttgg aaaatatctt ttcaataaac ttcgtaaatc
    17341 atcaccttgt ttgtaaccta tttcaaaata taatttacct ctaggttgca aatattctct
    17401 tgagttttca atgattttac gataaatagc taatccttct tcgtctgcaa aaagggcact
    17461 atgaggctct gaagctagca cattcttccc cacctcgtct ttgtcattgt aagaaatata
    17521 gggaggatta gacacaataa tatcaaattt atctgaaatg ttactaaaga catctgattc
    17581 tataaaagtc acttccgcat cacaattatt agcattttcc tttgccaagt ctaatgcaga
    17641 tacagaaatg tcactagcta gaacatccca agaggggcgt tcttttttta gagaaatggc
    17701 aattgctcca ctacctgtac caatatctaa aacagaacaa tcttctattt tattctctga
    17761 taagattaga tcaaccaact cctctgtctc aggcctcgga attaaaactc gttcatcaac
    17821 aaagaaaata agatctctaa agtaagcttt acctgtaata tactgcggtg atctatgttt
    17881 tttaagctgt tgaaaaatat tttccattaa ttgctgatca gtatcactca cactactatt
    17941 ttgatttaaa aggtaatcag ttgttgacca ttgttttagg tcttttaata cataaataaa
    18001 attttcgact tcttcaccac aagcctctaa caactgttca taatgcttga ttaactgcgc
    18061 ataattcatt atttatttaa ggcttctaac ttttgagtct gatcatacat aacaagtgca
    18121 tcaataactt catccatttt tccagataaa atagtatcta atttttgaag tgtcaaacca
    18181 atacgatgat ctgttacacg attttgtggg aaattataag tgcgaatacg ttctgaacga
    18241 tcaccagtac caactgttga tttacgttca gcatcttgtt catcttgagc aatttgcgca
    18301 aaatggtcag caacacgagc acgaatgatt ttcatcgctt tatcacggtt tttttgctga
    18361 gtacgctctt cttgcatttc aactttgatt cctgtaggaa tgtgtaccat acgaactgca
    18421 gtagcaactt tattgacgtt ctgaccacca gcaccagacg cgtggtaaat atcgacgcga
    18481 agatcttttt gatcaatttc gtattctact tcttcaactt ctggcattac cagaacagtt
    18541 gcagttgaag tatgaacacg accctgactc tctgtcaccg gaacacgttg aacacgatga
    18601 gctcctgatt catatttaag cttagaataa actgactgac cagaaaccat agcaaccact
    18661 tctttaatac caccaacacc attaacggat gattccataa cttcaaaacg ccatccttga
    18721 gtttcagcat atttttgata cattgttaaa agatcaccag caaaaagcgc tgcttcatcg
    18781 ccaccagcag caccacgaat ttctaaaata atgtttttat catcgttagg atctttaggt
    18841 aatagaagga tttttaatct ttcttcatac tcttctttag ctgcttttga ttctttaagt
    18901 tcttctttag ccatttcttc caattcagca tcaccagaag catccttgat catttcttca
    18961 gcatctgaaa tattttgaat gacttgctta tattctctat aagctgtgac agtttctcga
    19021 gtattagctt cttcacgtga taattccata aatcgctttg tatctgaaac aacatctgga
    19081 tcactaagta actcacctaa ctcttcataa cgatcttcaa ctgcctgcaa ttgatcataa
    19141 atattcattt ttctttgtat ctcctttaaa accgattatc ttttaaatat ctggattaaa
    19201 ataatgtttt cgacaaactg ggatatatgt ttcattacca ccaatttgta tctgatcacc
    19261 ttcatagaca ggttttccgt tttctgtcct taaaaccatg gttgctttct tactacagta
    19321 ttggcaaatt gttttgattt catcaatctt atcggccaat aatagcaaat gttttgaacc
    19381 ttcaaaaaga ttgttttgga aatcattttt caaaccaaaa gccatgactg gtacatctaa
    19441 gtcatctact actctagcta aatcgtagac gtttttctta ctcaaaaatt gacattcatc
    19501 aattaaaaca caataaggct tttgtggtaa attctgaata taggagaaaa tgtccatatc
    19561 atctgaaatt ggaactgctt cacgacgcat tccaatacga cttgaaacga ctccaaattc
    19621 atctcgagtg tccaaggcgc ttgtcataat aaccacaggt tttccttgtt cttcatagtt
    19681 atgagcaact tttaaaattt caatggtttt accagaattc attgtcccat atttataata
    19741 gagttgagcc aaagcggaaa atcctacttt ctatagtatt ctacctattt taccacaaag
    19801 gctgaagttt tcccagccat tgaaaacagt gtgtactttt tcacaacatt atgttacaat
    19861 gatgcccaat aacataaagg agtaatatta tgccatttgt aaaaattgac ttgtttgagg
    19921 gacgctcaca agaacaaaaa aatgaattag ctcgcgaggt tactgaagta gtatcacgca
    19981 ttgcaaaagc acctaaagaa aatatccatg tttttatcaa cgatatgcct gaaggaacct
    20041 actatcctca aggtgaactt aagaaaaaat aagaaatagt aaaagaggtt acccaggtaa
    20101 cctcttttga tagtctttgc aagcatattt cttgacaaat tgaaaatatt tgaaaataag
    20161 tattgatttt caagttagaa aacggtatca ttagaatata ttgctttggg ggtggaaaat
    20221 ttgcaacttt ctcatagttt aagactcatg ggaacgacta tcgatattca aattaattct
    20281 aaaaatgctc aaaaacaaat ccaagaggtc atagaactct tggagctcta caaaaatcgc
    20341 tttagtgcaa atgactttaa ttcagagtta atggctataa ataacaacgc tggtatcaag
    20401 cctattcaag tacatccaga cctctttgaa ttaatcacaa ttgggaaaga acacagctta
    20461 gctaggccta gtaacctaaa tattgctatt ggtcctttag tgcaaacttg gagaattgga
    20521 ttttcagatg ctaaactacc tagccctagt gaagtatcag aagctatgat tttatctgac
    20581 ccaactcata tacttctaga ttcaaaaaat caatctgttt ttttaaatca gataggaatg
    20641 aaaattgatt tgggtgcact tgcaaaagga tatattgctg acaaaattat gacctatctc
    20701 aaaaatgaga tgattgattc tgctatcatt aatttaggcg gaaacgtttt agttcacgga
    20761 gataatccta atcgttcaga agggtattgg gttatcggca ttcaacatcc gaaaaagaaa
    20821 cggggacaaa atattggagc tgtcaaaatt aaaaaccaat ctgttgtaac ctcaggtact
    20881 tatgaacgcc gtctgattat tgacgataag gaataccatc atatttttga tcgacagact
    20941 ggttatccga tacaaacaga gatggctagc atttctattg tttctaaaca atccgtcgac
    21001 tgtgaaatct ggaccacaag gttgtttggc ttatccatta aagaggctct tgatatttta
    21061 aacgctgttt cctacattga aggcattatc atcactaaag atgaccgtat ctatttatct
    21121 gatggcttaa aacatcattt tcaactattt tatcattaag aaagaggatt tctaatatga
    21181 aacttattgg catcgttgga actaactcaa ataaatcaac aaatcgtcaa ttattacaat
    21241 acatgcaaca acactttgct gacaaagcag aaattgaact catcgaagtt aaagaccttc
    21301 cgttatttaa taaacctgct gataaaaatg ttccccaagt aattttagat attgctgcta
    21361 aaattgaaga agctgatggt gttattattg gtacgccaga atatgatcat tctatacctt
    21421 cggccttaat gagtgtttta gcttggctat catatggtat ttacccactt ctcaacaaac
    21481 ccgttatgat tactggtgct tcatatggaa cacttggatc atcacgtgca caattacaat
    21541 tacgtcaaat acttaacgct cctgaactaa aagcaagtgt tttacctgac gaattcttgt
    21601 tatcacactc tctccaagcc tttgataaag atggcaacct acacgatatc gaaacaagtc
    21661 agaagctaga tgctatcttt gatgattttc gtctttttgt taagattact ggtaaattat
    21721 caaatgctcg tgatttactt caaaaagaag cagaaaattt tgactgggaa agtttataag
    21781 agtatagaag ggaataacta agatgaaatt tgtaggtatt gttggctcaa atgcagagca
    21841 atcatataat cgtatgttgc ttgaatttat tcgcaagaat tttaaaacca aatttgaatt
    21901 ggaagtcctt gaaatcgatg atattccaat gtttaatcaa gtccaaaatt gggaagaaag
    21961 tttccaactt cgtcttctta ataataagat cacacgagct gatggcgtta ttattgccac
    22021 tcctgaacat aatcatacta ttacggccgc tcttaaaagt gtgcttgagt ggctctcatt
    22081 tgctgtgcat ccactcgaaa ataaacctgt tatgattgtc ggtgcttcat attatgatca
    22141 aggcacatca cgtgctcaaa tacaccttcg taaaatcctt gatgctcctg gcgttaatgc
    22201 ttacacactt ccaggaaatg aattcttatt aggaaaagct aaagaagcct ttgatgataa
    22261 tggaaacatt atcaatcctg ggactgttca atttttagaa acttgtcttg ataattttat
    22321 caaatatgta ggagttgtat catcattgag aaaacctaaa gctattgaat ctgaagacct
    22381 ctactgtcag ggacaaatcg atacaaccat tactagtatc gatccagatg atcctgattg
    22441 gttagacaag gcagccgaga aagttaacgc cgttgaaggg gatgcttacg ttaaactaga
    22501 caatggttta cttactgtta accaacttaa tatgttttta aatgctatgc catttgagct
    22561 aacatatgca gatgataata atcaatttct atattacaat aataatcatc cagatccaga
    22621 tacaatgtta ggaaaacgtg ttcgtgaaca agtaggaaac cgtatggcca cagttcatgg
    22681 tagtttacca ccggcacgaa tgaaaaatgt tgaatgggtt atcggtactc ttcgtaatgg
    22741 aaatcaagaa tatgtacgta cagtcgttcc tggtacacct ccagaagtta tcaatactca
    22801 caactatcaa gctatgtact acccagacgg atcttatgct ggtattaatg aaattatctt
    22861 taactttaaa ccgtggttag attggtacct ccaaactact ggacaacgtt tggtcggtgg
    22921 aactactgct gctcctggag cttcagcagt tgacgctact tctagcgctt caactagcac
    22981 agaggatgct cccgttgatc aagccacaga tgccacttct ggtgcctctg aacattaatc
    23041 tcctcgcttt tgaggatctg ataccttaaa aagatatcaa gatcctcctt tttaatattt
    23101 aatcaagtta ataaaatcac ataaaaggaa tatactatgt cactttttca agaaaaaatt
    23161 gcttacaatt gcgctaaaaa ggaagcgctt tataaagaga gtttaggacg ctacgccttg
    23221 agatcaatgc tagcaggggc ttatttgaca atgagtactg ctgccggtat cgtcgcagct
    23281 gatactattg gtaaaatttc tcctgctcta tcaggttttg tatttgcttt catctttagt
    23341 tttggactta tttatgtttt aatatttaat ggtgaattgg cgacatctaa tatgctttat
    23401 ctcactgcag gagcctataa taaaaatatc tcttggaaaa aagccataac aattttaatt
    23461 tattgtactt ttttcaacct cgttggtgct tgtatattag cttggttgtt taatcaatcg
    23521 tactcattcc agcatttaac aaatgatagc tttttagggc atgtcgttgc taaaaagtta
    23581 ggaaaaccta gttcaggagc tttcctagaa ggtattattg ctaatatgtt tgtgaattta
    23641 gctatccttg cttacatgtt attaaaagaa gaatcggcaa aaatgacggt tattttatct
    23701 gctattttta tgtttgtttt tctatcaaac gaacacttaa ttgctaattt tgcatccttt
    23761 atgttagctg cttttagcca tatcgaacat attaaaggtt ttactcttct aaacgttatc
    23821 cgccaatgga cacttgtatt ttttggtaac tggataggag ggggcgtttt catcggccta
    23881 gcctatgctt ggttaaataa aacaaaaact tctcaccatg aataaaagaa aaactgagta
    23941 atttattact cagtttttct tttatattct catcatgaaa tcaataacta aaaattaact
    24001 tttttgataa ataaaggaaa gtatcatatt aattttttag gcaatccatt caaaatgata
    24061 tttaaaacaa cagctgtcag ggttgagata acaataccat ttgtcaaaaa catttgtaaa
    24121 gtattaggaa gactaatgaa gagatttgtc tcattaaaac ctactcctgc tgcaattgat
    24181 acggctgcaa tgataaagtt atgctcatta tgctcaaaat caacctgatt aagcattttc
    24241 atcccttgaa gtgctaccat accaaataaa actaacatag caccaccaag aactggactt
    24301 ggaatcattt gagccattgc gccaaattta ggtaacaagc caagtataac taagaagaga
    24361 gctgtaaaat atatcggctt acgcgtacgt atcccagata tctgtaccag tcccacattt
    24421 tgagaaaaac cagtatatgg aaaggtatta aataagccgc taagtaatac tgccaatcct
    24481 tctgaacggt aaccattacg aagtctctta ctgtctaatt tatcgtttgt aatatctgaa
    24541 agcgcaaggt aaacacctgt tgattctacc atagaaactg ttgcaataat acacatcatt
    24601 aaaatagaag taaattcaaa tctaggagct ccaaaataaa atggcttcgg aatatgtaca
    24661 agtggtgctt ctgctaccac agaagcatca actaagccca taaatgctgc aagaattgta
    24721 cctgaaatga gtccaataag gattgaaatt gacttcaaaa agccttttgc gaaaatatta
    24781 attaataaaa caacaccaat cgttaccagt gataaagtta aagattgtaa gcttggttct
    24841 tttgcattat cacccatatt tcccatcgca actggtatta atgtcaaacc aattgtagta
    24901 ataactgatc ctgttacaat tggaggaaag aaatttgcaa ctttagaaaa aatgccagca
    24961 actaaaacga catatattcc tgaagcaatt aaagctccaa acatatagcc agaaccttgt
    25021 tgtgcaccaa taattgataa aggagctaca gactgaaagg cacaccctaa tacaactgga
    25081 agacccactc caaagtgctt acttaatcgt aattgtaata aggtggcaat cccacacata
    25141 aaaatatctg tagcaataag ataagttagt tgcttagcat tataaccaag agcactagca
    25201 atcatgatag gtacaagaat ggatcctgca tacatagcta acaggtgttg caagcctaga
    25261 agtgctgctt gcgaattact ttgctcatta aatttcatta agcatcagcc tccgcaaaaa
    25321 caactcgacc attttcaaac gctttaatac gagcaagaga agtaaccgga acacctgttt
    25381 tttctaacaa atcacgccca tcttggaaag atttttcaat aacgattccg ataccagcaa
    25441 ccttagcttc agcttgacca ataatttcaa gtaatccttt agccgcttga ccgtttgcta
    25501 aaaagtcatc aatgatgagt acagtatcat cgttagataa aaagcgactc acaatagaaa
    25561 cttgactcgt aacttgcttt gtaaaagaat acacttcagc agttaagata ccttcagtca
    25621 tagtaatgtt cttagctttt ttagcaaata tcattggtac gcccaatgct tgagctgcgt
    25681 acactgctgg cgcaattcca gatgcttcaa ttgtaacaac cttcgtaatg ccggcttctt
    25741 tatatttatc agcaaaaact ttacctattt cctgcattaa ctcaaaattt acctgatgag
    25801 tcaaaaaaga atcaactttt aaaatgttct cacctaaaac atcaccatct ttaagaatac
    25861 gttcttccaa taacttcata ataacccctt cactctttag aaaataaaaa aataacttac
    25921 ctcaatcaga ggacacaaat ctcctctaat ttatcgcttc attaacatga accaacaggc
    25981 aagctattta ttagcttact tattgttaga cattacggtg tcttgtagag acgctaagcc
    26041 aatcctcagc ataaataagt aaatcatata tttaattgtt atgtatgata gataactatt
    26101 gtaacatttt taattaagat ttcaagattt aatttaaatg gaatcaccat tccaaataga
    26161 atttttaaca ataacgtaat caacgtgtct caagctatcc aattcttttc cacctgcgta
    26221 agaaatagaa ctttgcaaat cttgttgcat ttctgtcaaa gtatcctcta gtcgcccttt
    26281 aacaggtaat agaattttct taccttcaac atttttatgt tctccctttt gatattcaga
    26341 agctgagcca tagtattctt taaattgctg tccttctact tccacaagtt ttccaggact
    26401 ttcaaggtga ccagcaaaca gcgaaccaat cattaccata ctagcaccaa agcggataga
    26461 ttttgcaata tccccatgtg ttcgaatacc accatcagca ataattggct tgcgagcagc
    26521 tttagaacac caacgtaagg cagctaattg ccaacctcca gtaccaaaac ctgtcttgac
    26581 tttagtaata caaactttac caggtccaat accaacttta gttgcatctg cacctgcatt
    26641 ttccaattct ctaacagctt caggcgttcc tacatttcca gcaataacga aagttcctgg
    26701 aagctcttgt ttaatgtgtt ggatcatctc tataacactg ttagaatggc catgtgcaat
    26761 atcaatagtg ataaactcag gagcatcttc tttcagactt gtcacaaaat catattcata
    26821 gtctttgacc ccaaccgaaa tcgaagcaat taagccttta tcatgcatac gcttgataaa
    26881 aggtcttctt gcttcttcat taaaacggtg cataatataa aaataacctt cacacgctaa
    26941 tgtttcagct acctcttcat caataatagt ctgcatatta gcagggatta ctggaagttt
    27001 aaaggtatag tttccaagtt tgacacttgt gtcagcttgt gagcggctag atataataca
    27061 cttgtttggt attaattgaa tatcttcata atcaaaaaca ggaatatcgt taaacataat
    27121 ggttctccta atgagtaaaa tgaccttcct ataactttgt ataacaaagc atcgctaggt
    27181 aaaaacatac ctttcctttt taaattatat actttattta tttttagtca atttttgatc
    27241 aatttatttg aataatgttc gtttttttca aaataaatgt aaaatcctat agagcagtac
    27301 tctataggat tttacattta ttatattgct tttctttttc gaagataagt taatcctaca
    27361 atagctaaga taacaacaac gataacgaat agaactgcaa attgactacc aattatagct
    27421 ccatttttcg gaatagctac ttgtccgatg gtaaaaattt gtgaggctac tgcggttgca
    27481 aaagctcccg caaattgttg tagtgtattt aagatactat taccatccgc tgactgtgct
    27541 ggctttagtt ttgttaaagc tgtcgtaacc atattacttg ctccaatccc cataccaatt
    27601 ttagtaagaa tatcaaaggc taaaagtact acaacaaact tcgcaggaat gaatatagct
    27661 gtaccaatta aacctatcaa agaaagggag atacctatca taattggctt aaatgcacca
    27721 attttatcca ggagataacc tgcaaaaggg gataaaaaaa ctactgctat tgcacctgga
    27781 aacataaaca aaccagcttt ggttgaagtt tgatgtaaaa caagttgcaa gaggttaggt
    27841 aataagaacg aaagtgctag aggaatcatt tgaaaagcca ggacgccata caataagatt
    27901 gagaaatctt tatctttaaa aacattcaaa ttcaaaaatt ctagctgttt atttaaatag
    27961 taaaaaataa caaaactaac aatagctact aataagtaaa aaatagacat cttttcaata
    28021 gctaaaagaa gggttgccaa gccaatacct aaactgacaa aggcaggaaa attgagttta
    28081 tcattaattc ttacttgacg tttgggaata gaagccaaac ctataaaggt agatatgata
    28141 agtattggtg ccagaagcat aaaaatcatc ttccatccta acattcctga aatgacacca
    28201 ccataagtag gtccaactgc cggtgctaaa agtgtgacca tagcagctac tcccatcatt
    28261 agtcctctac gctccatcgg aatttgtgta agaatgatat gaaacaataa agggagggct
    28321 aatcctgtcc caacaccttg caagactctc cctactaaca atatagctaa attaggggca
    28381 aagctatcaa ttaaaacacc aaataaaaaa agcacatttg ccaatgtaaa aatttgtctc
    28441 tctgataaat ttttaatcat aaatgctgac aaaggcacag tcacagcaac agctaacaaa
    28501 tttcctgttg tcacccattg aataacagcg gggttaaccc caaattcttt catcaaaagc
    28561 ggaaaagtga cattcataga tgtttcaata agaatccctg aaaaacctaa tatagccgtt
    28621 gtaattatgg caggcataat acgccatccc tttttagtca ttaaaatctc cttactttga
    28681 ttattatcat aaaatagagc accccgctaa atcaaggtgc tccatttaat tcattcgaat
    28741 taaaattaaa ttgtttatat tagttgtcaa aggacaatca acagctctta ttatatcaaa
    28801 taaagctaag aaaaacaaag agttttttga atattaatgc cttcactttc ttatctattc
    28861 tctacccatc aatcaccact tcagagaaca taacaattgc aatttactct taataccgtt
    28921 atcatgttca tatgctaata cccaagaaaa tagtggcttt tgcctcaaat gaatctgaaa
    28981 ataatttctt tattacgtta aagacttact ttaattattt gttttctatt caaatcatta
    29041 cagatatttc aactctcaat catgcagatt ttgacggtag ctttgcattc catgatatag
    29101 aaactagcat acctcatctg gtaattgact ccaactactt agcgattagt cagactaact
    29161 caaaaacaga ggctaacgat attaaaacgt tctcggaact ctccaaaaca atgaccgagt
    29221 tttactatat gctaaatttt gacttattta accatctccc atatcgtttt agattgcata
    29281 ataaggacgg tcaaacaatt tattcgaacc ataaacctga agatcctttt gatatttatc
    29341 ctgaagaaga atatcctata gacaaatggg ttcaaaacag tctaatagag aaaaaagcga
    29401 aagagctaca tttactctta cctggcgcat cacaagatta tattctagta caatcttata
    29461 aacgcttaga aaatgatagt gggcaactag ttggttatat tgaacatgtc cataatatta
    29521 aaccactatt agaggggtat ttaaaagaat ccggtcaagc tatcgttgga tggtctgatg
    29581 tcacgtctgg tgcttctatc tctaacgatg attttgaact ataatccact cgtcactcta
    29641 aaaagaaaac taataactaa aaggcactgg tttaattcca gcgcctttta ttaatcaatc
    29701 tccagttgtt gtaagttcaa ttttttcttt aattggtaga cctcgattag ctgtaaaaat
    29761 caaaggaact acttctgttg taacttcgga aaattgtaga ccaaaccatc gtattggtgt
    29821 tgctgtccaa tgctttacag atgatttcat tagcataaca aatcgttcac caggttttag
    29881 gttttgactg cttgaaaaac gttcatcaag gacaataaac ctaaaatctc ctactttact
    29941 atcaggtcta acgctgtatg tctttccttg tttaggcaga cgtccacttt caagtaattc
    30001 ttctacaatc gtacgaagat agcgtgacac agtctgacgc atcttaaatc caaggtaaag
    30061 ttctaccttc acaatgaagt cggttcccat catgtcaact ttatactcag ctgtataagg
    30121 ttcatcagtg accttaatgt ttacaaacca ataaacttgt gcttttttag ggcgcttatc
    30181 aaggatagaa tacatgatag acttatcaat catgtctcct tccatacgat ttgtcaaata
    30241 aacaacgtta gtatgataaa tcggataacg atgatcatgt ctaagtttat ctaattgtcc
    30301 gatgtaatct tttaaatcca acaacttaac ataacgcgaa acaattttac tacctttata
    30361 ccagatagtc atgatgaata taattgctac tgctataata acaacaacat agcctccatg
    30421 cataaattta acagctgaag caaggaagaa gattccctct aaaataccaa agaaaatcat
    30481 catcaataag actaaacctc tttttactcc tttttgaatt aaaaagaaag acagtaaaat
    30541 agttgtcatt agcatcgtaa ttgttatcgc taatccatat gctgcttcca tgtgcgctga
    30601 agtcttaaaa agcaaaacaa tagaggttgt aatggcaaat aagaaccaat taataacagg
    30661 tatgtaggtt tgaccaatat tgtcaccagg ataagttgaa cgaaattgtg ggaatatttt
    30721 tagtcgcata gcttcagaaa ctaaggtaaa tgatccagaa atcagtgcct gtgaagcgat
    30781 gatagctgcc aaagtagcta aaataacgac atgcattgta aattgcgaag gaatactagc
    30841 aaaaaaggga ttcaattcat ttcctgcgtt cttattagct aaaatccatg ccccttgccc
    30901 acaataagaa agtataatgg caaccttaac gaacggccat gaaacatgta tatttccacg
    30961 cccaacatga cctaagtcag agtatagtgc ttctgctccc gttgtcgcca ggaaaataga
    31021 tcctaaaata aaaatacctt tatgattctc tggactaaat aacaatttca aaccgtagta
    31081 tggattaatt gctttgaaaa cttctggatg ggcaaaacta ttaaggagac cgctaatacc
    31141 taaaaaggca aaccatataa acataattgg cccaaataat ttcccaataa cacccgttcc
    31201 aaacctttgg atggcaaaaa gtaacagtaa aataaacaag gtcgcaaaaa taacattact
    31261 ctgattctgg aaaatatgct gcaaactagg aactactttt aatccttcaa cggctgaagt
    31321 tacggttaca gctggcgtca aagctccatc tgacaacaag gttgcacctc caataacagc
    31381 cggaacaatt aaccaaggtg tcatttttct aacaagggta tataaagaaa aaataccacc
    31441 ttcgtggtga ttatccgcct ttaaagctac aagcacatac ttgatagttg taataagtgt
    31501 caaggtccat atgattaaag atatagaacc taagataaac gattctgtga cactagaaat
    31561 accaccttgg ttttcaacca atgattgcat cgtatagagt gggcttgtac caatatctcc
    31621 ataaacaatg cctaaagcaa taataaatcc tgcttttgat gctttatcaa aagaagaatg
    31681 attgacatgt tgcatttctg ccaaccctcc gattaaataa tattagtcaa aaaaatggct
    31741 gaactcatta tagcagatta agttaaaaaa acaacactta tctataaaaa gaccagaaat
    31801 tattaaagcg tttcctggtc tttactattt taatcacgat aaacaggttg aggtccataa
    31861 gtttgactga ctggcattat ttctatgcga ttaatattaa tgtgttctgg ttggctagca
    31921 acccatgaca cggtattagc aatatcctct gcttgaattg catgcgcacc ttcataaagt
    31981 ttttcaactc ttttatgatc tcctttgaaa cgtacagttg aaaattctgt cccttcacat
    32041 aaaccgggtt ctaggttagt aactctgatt ttagttccag ctaaatcggc acgcaagttg
    32101 agagcaaatt gttttacaaa agcttttgat gcgccataga cattagctcc tggataagga
    32161 attgttcctg cagttgatcc caaattgata attaaccctc tattaacttc taccattttc
    32221 ggtaaaatgc atcgtgtcaa gtaaattaat cctacaacgt tagtattaat catagtcatc
    32281 caatcttcaa aatcagcttc atatgattta tccaatccaa gtgctaaacc tgcattatta
    32341 accaacaaat caattgattg caaattttta ggcagacttt ctaaagcttt gtcaaccgat
    32401 tctctacttg taatatccat ttaaagtgga taaaagtaac cctctgaaaa cttttctcca
    32461 aggcatttta atttatccat tcgacgagca caaccaataa cgctataacc atctgaaact
    32521 aatttagtaa caacagctgc accaaaacca gcagatgccc ccgtcacaag cgcaacttta
    32581 cgtgatatca taaatactcc tcctctagta tatagaaaaa cgagctagga aaaatccttc
    32641 tcgttttata acttattttg atttctcatc aatagcttga gcagctgtaa tgatagcaag
    32701 tttgtaaata tcttcagcac ttgatccacg tgataaatca tttactggag cattcaatcc
    32761 ttgtaagatc ggtccgattg cttcaaacat acccaaacgt tgtgcaattt tatagccaat
    32821 atttcctgat tgaaggtctg ggaaaataaa aacatttgct tttccagcaa catcactatt
    32881 aggagcttta atttctgcag tttccggaac aaaagctgca tcaaattgta attctccatc
    32941 aacagctaat tcgggagata aatctttggc aattttagct gcttcttgaa ctttttcagc
    33001 ttgaggcgct tttgcacttc cttttgttga aaaactaagc atagcaatct tgggattaat
    33061 atcaaatatt ttggctgtat ccgcagtatt tactgcaatt tctgctagct cttgtgcatt
    33121 aggatcaatg ttaatagcac agtcagcaaa aatataacgt tcttgtgtat tttcacggtt
    33181 catcaagaat acacctgaag tgcgtgaaat tcctggtttt gttttaataa tttgaagagc
    33241 aggacgtaca gtatcagctg ttgaatgaat ggcccctgat accataccat ctgccaaacc
    33301 aagttttact aacataaccc caaaatagtt aacatccttc aaaagacgat ctgcatcttc
    33361 catagtagct ttgcctttac gaatctcaac aaaagcttct ttcatctctt caaatttttc
    33421 gtattgttca gggtcaataa cgctataatc ttgatcaaca aaaccaagtc ttgccaaaga
    33481 atcgtgaact tcgtcagcct tacctaaaat aacaggatcc acaagacctt caaatttcaa
    33541 acgagctgct gcacgtaata cacgttcatc gcttccctca gggaaaacaa tcttaacata
    33601 atcacttgca actttttctc ttaaaccacc aaataaggaa cgcatatata taaaaccttc
    33661 tatttctaaa ttaatgtaat tccatgataa cgctgtcaaa gtcatctgtc aagtctagcg
    33721 tctgtttatt tatttttccc ataaaagggt cgtaaaaaga caaagaatga caatgcaatg
    33781 cttgcctgtt tattcctaga tccatacgtc ctccgtacaa atcatctccc aaaagaggaa
    33841 agccgatatg agcaaagtga actctaattt gatgagtacg ccctgtatgt aatttgatat
    33901 ccactaatct aacatcacca taacgagcaa ctacttgata actagtatgt gcatatttgc
    33961 ctgattcatg gactcgcctc gtaataatac tatcaacatc tcgtgcaata ggagctataa
    34021 tatcaccact atctgctaaa tcaccactcc ctgatactaa tgcataatac cttttttcaa
    34081 ttgctttagc ctgaagttgt ttatctagac gagcatgggc ataaccatgc ttggcgaata
    34141 acattaatcc acttgtatct cgatccaacc gggtcacaat atggacctgc tgattggcat
    34201 agttattaga cacataatag tgttttataa aattagcaat tgtatttgaa tgaatagagc
    34261 taggaatact agcgaaacca aaaggtttat ttaaaatcaa aaaatgatca tcctcataaa
    34321 caatatctaa atcgtgaggt actggttcca aagtttcgtg agaaggctcg tcaggaatat
    34381 ctatcgtaac aacatcgcca atctctaata agaaaatagc gttctgctct tcaccattga
    34441 caaaaatctt accacctctg tacttaatct ttgctaataa cccccttgaa acatcatgac
    34501 ttttcaaaag tgttttaact ttgcaccttt cttctgctat gtattcaaat ttcaatgttc
    34561 aggttctccg ataaaagcat cgttaacacg ttcccaaaag ctagtgtgac ttggagttga
    34621 aacaaagtta atactttttt cgtcaatact atattctatt ttggtaacat ttttgtaatg
    34681 gaccgttttg ttatctatag aaattgtata gacaccaact cgtttaggaa ctatctctat
    34741 agcatctttt ttggggatga taaccgatga ccccaaggta cgatagacgc gattattaag
    34801 actagaaatt tctgttagtt gcaaggcttc tatcgttggg tggagtactg ccccccctag
    34861 tgatttattg taagcagtac taccggttgg tgtagaaact aaaatgccat ctcccctaaa
    34921 cctttcaaat accacttgat taataacaac atccgctacc attgtttttt caatacgctt
    34981 aatagtagac tcatttaaag cacgcgcacg aataactcga ccatcttcaa gggtaatagt
    35041 tactttcaaa ataggatagg agatttgttc acctttatca tttttcaaat tattaattaa
    35101 tgtgtcaact tcaaaatctc gatagtctgt ataaaaccct aaatgtcctg tatgaacacc
    35161 gacaaacctt actttatcca gttgtttttc gtacatatgg aaggctgaca aaagcatacc
    35221 atctccacca atagaaatga caatatcagg atcctttttt gataaataaa aatcaggatc
    35281 atgtttaaaa gcagcaaaga gctttgaggc tacacgcttg ctttgatatt ttccgttagc
    35341 aattatagca acacgtgttg ctctatctgt aaaattcatc tgtgtcatca ctatttccta
    35401 caccatcgct caattttcga tttaaaggat caaataaaag ctgtgcttca cgaatatctt
    35461 cgcgaatctt acgcatttct tcatccaatt ctaaagcgat ttttgctgtt ttctccagtc
    35521 tttgtttaat ttcttcaggg aagtccccct gatacttgta atttaatgaa tgttcgatgg
    35581 ttgcccaaaa attcatagcc agagttctaa tttgtatttc tgctaaaact ttcttttgtc
    35641 catctattgt gtctacagga tactctacaa cgacatgata tgaacgatat ccactagatt
    35701 tcatatgggt aatatagtct cgctcctgaa ccactgtcat gtcatgacgt tttctcaaca
    35761 aggcaaggac ttcatcaacg tcatctacaa attgtaccat gatacgaaga cctgcaatat
    35821 cttgcaagtc ctgagcaaga ttttcttctg aaataccacg taaaaccatc ttttcttgaa
    35881 tactttcaac cgactttacg cgtccagtaa caaattctat tggagagtga cgattttgct
    35941 ttctaaattg tttacgaata ccacgaagct ttatttttaa ttcaccaacc gtttgaatat
    36001 aagggtctag aaatgtttcc caatccattg acattctaat cctccaaaat atttaatgta
    36061 ctctcttgta aaataaactt tttttctata aaattaacac aacaattaat attataccat
    36121 atttaggaaa aggaagtgat aagatgacac acctagaaat tgaatacaaa actctattga
    36181 ataaagacga atttaatcgt ctaacaagct tattttctca tgttcaacct attactcaaa
    36241 ccaattacta ttttgacaca gaaacatttg aaatgaaagc acaccgaatg tcacttcgca
    36301 ttagaacgtt acctaaccgt gccgaactaa cactaaaaat ccctagagaa gtaggaaatt
    36361 tagagcacaa tcatgacctt actttagaag aagctaaata tatcgttaaa aatggccagt
    36421 tccctgaaga tactgagatt gcttctttaa tccttgaaaa gggagttgat ccaaccaaat
    36481 tagctgtctt tggacaactt acaacaacaa gacgagagat ggaaacttca atcggcttaa
    36541 tggctctaga cagcaatata tatgctgata ttaaagatta tgaacttgag ttagaagtca
    36601 aacaacctaa acaagggaaa agagatttcg accagttttt aaaagaaaat aatattaatt
    36661 ttaaatacgc caaaagtaag gtggctagat tctctgctac tttaaaaaat aactaacgtt
    36721 gagagattgg attttccaat ctcttttata attctcattt aagcaagaac ttaaaatctc
    36781 atactatctc tcctcaacat tgttattgca agcattttca tgtttcttat cgtgaatttt
    36841 ttctattttt ttggtaaact attagtatca atctaaagga gtcatctatc tcatggcaga
    36901 acaatacgcc gataaacaaa tcaaactttt ctcattaacc gctaatagag aaattgctga
    36961 aaaaatttcc caagcttcag gaattccttt gggtaagatg tcatcacgtc aattctctga
    37021 tggcgaaatt atgattaata ttgaggaaac ggtgcgcggt gacgatatct atatcatcca
    37081 atctacaagc ttcccagtca atgataatct ctgggaatta ctcattatga ttgacgcttg
    37141 caaacgtgca agtgctaata ctgttaacat cgtagtccct tattttggat actctcgtca
    37201 ggaccgcatc gctgcatctc gtgaacctat tactgcaaaa ttggtcgcta atatgttagt
    37261 taaagctgga gttgatcgag ttcttaccct cgacttacat gcagtgcaag tacaagggtt
    37321 cttcgatatc ccagttgaca atctttttac tgtcccacta tttgctgaac actataatca
    37381 actcggtctc tctggtgaag atgtcgtcgt tgttagtcct aaaaattctg gaatcaagcg
    37441 tgctcgtagt ttggcagaat accttgactc tccaattgct atcattgatt atgcacaaga
    37501 tgattctgaa cgtgaagaag gctatattat tggtgaggta gaaggtaaga aagctattat
    37561 tattgatgat attttaaata caggaaaaac ctttgccgaa gctgcaaaaa ttttagaacg
    37621 tggtggagca actgagattt atgctgttgc tagtcatggg ctttttgctg gcggcgcagc
    37681 tgatatttta gagtctgcac caattagaga aattatcgta acagactctg ttttatctaa
    37741 agagcgtatt cctagcaata ttaaatatct gaccgctagc catctcattg cagacgctat
    37801 tatccgtatc catgaaagaa aacctttaag tccattattt tcatatcgtt cagataagaa
    37861 ggattaattg atgatttatt tagacaatgc tgctactacc gctctaaccc catctgttat
    37921 tgagaaaatg accaatgtca tgacaagtaa ctatggtaat ccatctagta tacatacctt
    37981 tgggcgtcaa gcaaatcaac ttttacgtga atgtcgacaa attattgctg aatatctaaa
    38041 tgttaattca cgtgaaatta ttttcacttc tgggggaact gagagcaaca atacagctat
    38101 caaagggtat gctcttgcaa atcagctaaa aggtaaacat attattacct ctgaaattga
    38161 acatcattca gtcctacata ctatgactta cttatcagag cgatttggtt ttgatattac
    38221 ttacttaaaa ccaaaccatg gacaaattac tgcaaaagac gttcaagaag ctttacgaga
    38281 tgatactatt atggtatctc tcatgtttgc taataatgaa accggggact ttttaccaat
    38341 tcaagagatt ggtcagcttc tcaggaacca ccaagctgtt tttcacgttg atgccgttca
    38401 agtctttagc aaaatggaac ttgatcctca ttctttagga attgactttt tagctgcttc
    38461 tgcccataaa tttcacggtc caaaaggtgt tgggatactt tactgtgctc cccatcactt
    38521 tgatagtcta cttcatggtg gagaccaaga ggaaaaaagg cgtgcttcaa ctgaaaatat
    38581 aattggtatt gctggaatgt ctcaagctct tactgatgct acgactaaca cccttaaaaa
    38641 ttggactcac attagtcagc tgagaacgac ctttttagat gctatttcag accttgactt
    38701 ctatcttaat aacggtcaag actgcttacc tcatgtactt aatataggtt ttcctagaca
    38761 gaataatggc ttgttattga cacagttaga tttagctgga ttcgcagttt caacaggttc
    38821 tgcatgtact gcaggaacag tcgaacctag tcatgtctta acaagcttgt atggagccaa
    38881 ctcaccacgt ctaaatgaat caatacgtat tagtttttca gaactaaata cccaagaaga
    38941 aattcttgaa ttagctaaaa ccttaagaaa aattatagga gattaatatg gcatttgaaa
    39001 aagaaataaa tcttccagac tgcaattaca tttacactat cagcccgaac attaaaaaat
    39061 ataccttacg cgatactaca ttcactcaaa atcgcttagg taattatgaa ttagagcgtc
    39121 tcctagaaac agtacctaat tctggcgatg gctttcctct aaagattaca atcaataaag
    39181 aactttctgg ttttaagctc ttaattactg ataaatctgg ccttagaata gtaaatatct
    39241 ttaaatctac cgatcataaa attcttcaag acaaatttta tttcttaatg gacagcctcg
    39301 ttgagcgtaa tatctttacg aaaactgcaa aataatttac cgaccactat ttttaacatt
    39361 ttagtggtct ttttgcgaac cattttgttg tcactttcac aatctttttg tacaatagag
    39421 atgtgaagaa ttaagttata taactgattc taaatcgaat aataaaattt ttaacaagga
    39481 gataattttg tgattatgga taagtctatt cctaaagcaa ctgctaaacg tttatcactg
    39541 tactaccgta tttttaaacg ttttaatact gatggcatcg aaaaagctag ttccaaacaa
    39601 attgcagatg ccctaggtat cgattctgct actgttcgac gtgatttttc ttattttggt
    39661 gaactaggac gccgtggttt tggttatgat gtcaaaaaac ttatgaactt ctttgcagaa
    39721 atattgaacg atcattctac aacaaatgtt atgctggtgg ggtgtggaaa tatcggtaga
    39781 gctctcttgc attatcgttt ccacgatcgc aataaaatgc aaatttcaat ggcttttgat
    39841 ttagatagca atgatttagt tggtaaaaca accgaggatg gaattcctgt ctacggtatt
    39901 tcgactatca atgaccattt aatagatagt gatattgaaa ctgctatcct aacagtacct
    39961 agtacagaag cccaagaagt tgctgacatc ttagtcaaag caggtataaa aggcatcttg
    40021 agtttttctc cagttcattt aacattacca aaagatatca ttgttcagta tgtagattta
    40081 acaagcgaat tacaaacttt actttatttc atgaaccagc agcgataaat agttcattat
    40141 ttataaaata tctgcttctt ctcgaaaact ataatattta ttttttccaa cgatgatgtg
    40201 atctaggcag acaattccca gatgatcaca tgatcgtttt atttttttag tgaaacttaa
    40261 atcactttca ctgggatttg gagaacctga gggatgatta tgtataataa tcaaagaagt
    40321 tgccatgttt ttacaagcat aatgtagaat ttctcttggc tctgctactg aacgacgtac
    40381 agtaccaata aaaatagttc tctgttcgat aatacgattt tgtgtatcca tataaatagc
    40441 tactaaatgt tcttgttttt tatcccctaa ttcgagcatc attttcctcg ctaattgttc
    40501 actacttaaa atttgctctt tacgatcata ttcagctttg tgaatccgtt ttgctagttc
    40561 tagcatagct tttatttcga cggatttaac ctgaccgatt cctttaatgg attgcaactc
    40621 ctgtaaggat aattgaccaa aatctgctaa actgcttatg ttttctaaaa tttgcgttga
    40681 aatttcaaga acaggttttt ctttaatacc tgtacgtaag agaatggcta ataactcctg
    40741 attactcaat ctatctgcgc ctaaatcaac taggcgttct cttggtagta aagcttcctt
    40801 ttttaattca atatggtaca taaaacacct ccagtcataa tattcgtaaa ctaatcaaaa
    40861 actcatgttt taaatcaata aaaaatactt agatgaaaaa tctaagtatt ttttatttaa
    40921 cgataggttc gtgcacgatt tctaagcgtt tgtcgcttga taatatgatc ttttacaatc
    40981 tgatagatag tagctgcaac aggaacagca agtaacatgc ctaagatccc ccataaagca
    41041 cctccgatag taatagccat taaaacccac atagaaggca gtccaatcga tccaccgaca
    41101 actttcggat aaatgacatt tccctcaaat tgttgtaaaa ggatcaagaa aagaacaaac
    41161 aagaatgctt cagtaagcga ttcagtagca attaagatga aaccgattgt aacaccaatg
    41221 taggctccca caaccggtat tagagctgta aaagcaacta aaaccccaac tgttaaagca
    41281 taaggaaatt ggaagatcaa cataccaata accgtcaaac ttcctaaaat catagcttct
    41341 aaagtttggc ttacaaaaaa accatggaaa cgttgatgaa ggatatgacg aacgtaatgg
    41401 aatgttttgc ctgttgaacc taaataggta tcaattaaca aattaaattg acgtcccaac
    41461 tgctccttgt ttgccaaaac gtaaattgaa aaaataaaac taacaaaaac attcagaagt
    41521 gttgccgcaa tagaggaaac tgaggttagt aaatttgtta aaacagataa aacttgcttc
    41581 aaaatctgct ggctataacc acttaaagta gaaactaagt ctttatctgt tcccatataa
    41641 tttaaagcct cagaaatttg tttattttca ttgagattat taaccaattt agcaagtgct
    41701 ccggtatcaa taaccaataa agaactcaaa ctagaaatca aatctggaat gacaattgaa
    41761 aatagccaga caattaaacc aataaaagtt gcataggata aaatcataga aacactacgc
    41821 ttgattgcca ttaatagtct agatc
//
LOCUS       DATROY010000007        36479 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.007, whole genome shotgun sequence.
ACCESSION   DATROY010000007 DATROY010000000
VERSION     DATROY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36479
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..201
                     /locus_tag="VBD11_000309"
     CDS             <1..201
                     /locus_tag="VBD11_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953288.1"
                     /translation="WSKSPYSESFYSSKDIDWGYKPEGSLRVSDHWDFGDEEEHCPTV
                     EKVQGWAVCKFENGIYHLVEKF"
     gene            210..593
                     /locus_tag="VBD11_000310"
     CDS             210..593
                     /locus_tag="VBD11_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953289.1"
                     /translation="MSWKKIHFNSMNIIYENKKNCLIKMPNNSNYKNYKFWHPSKLVR
                     TLERGNGYFKTFSFTDEWEFNIFKDDKDYNKTKEITLSSEDMELEFETTNIEVEHNSN
                     SKSFYEEHEPVKIEKEVEVLDELTR"
     gene            577..1836
                     /locus_tag="VBD11_000311"
     CDS             577..1836
                     /locus_tag="VBD11_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953290.1"
                     /translation="MNSQGDFVLLTEDQQKAIEKFSRLKVGALFMKQGSGKTRVALEL
                     VRTTDSEFVLFMCPFSVKKNLLDEINLWGIDKEFEVIGYESISSSDKEYLRILDKLKN
                     AYKPFIVADESVFIKNDTSKVHRRMLEFRDLSEYRLILNGTPITRDEWDIYNQMEFLS
                     YKIFNMHRHEFLSTFFKTIRYKKKGFNVREFKKLSDVNIDYMRKLIAPYIFEADLKFD
                     KKETEKGISVDYSDECYEKYQERKESLLNSLEYGLSVVQQFANLAVACFDDKKRHEEI
                     AKHLKGQVIVFCTLLSEVKNISNKIDCYVITGETKPKDREKILEDFKNDNKPLLLTFG
                     TGAFGLNLQFCNRIAFASLTFDYARIDQAMSRIKRLGQSRDIEYTYFTSDLGVYRMIK
                     ENIFKKQSLKELIIDKIDKGELNENSL"
     gene            1820..3091
                     /locus_tag="VBD11_000312"
     CDS             1820..3091
                     /locus_tag="VBD11_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HEO6953291.1"
                     /translation="MKTVYDLAMSRIKYIFDNFDHVYISFSGGKDSGVMLNLALKYLK
                     DNKLDKKITLMHLDYEAQYEMTTEYVEKMEKEFSSYLNVYHICVPFKVTTCTSMFQNF
                     WRPWEEGKEDIWVRDLPENAKTKEDFPFFKENMWDYEFQEKLSTFIHDKEKAEKTAVL
                     VGIRTQESLHRWRAIHKDRDTYYNNKKYSKKISENVYNFYPIYDWKTEDIWVANAKFG
                     FAYNKLYDLYYQAGMSIDQMRVASPFLSEGQETLKLYKVIEPHTWGKLVSRVNGVNFT
                     GLYGGTTALGWKKITKPAHLSWKEYMEFLLDTLPEETKQSYLSKLETSIKFWKEKGGV
                     LSDEVIKELDGLSIKYEFGTHGYNTNKKAVKLDYLDDLDIKDFKAIPTYKRMCICILK
                     NDHLCKYMGFSQTKNEMKKRKEAIEKYANIL"
     gene            3075..3590
                     /locus_tag="VBD11_000313"
     CDS             3075..3590
                     /locus_tag="VBD11_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEO6953292.1"
                     /translation="MQTFYSPVYNVKPVPIEKIQANSYNPNSVASPEMKLLYQSIKQD
                     GYTMPIVCYYLKDIDKYEIVDGFHRYTTMLKHKDIYERENGCLPVTVIDKPLSDRMAS
                     TIRHNRARGSHDIGLMTNIVADLVDSGMSDAWILKNIGMDADELLRLKQVSGLAALFS
                     NKEFSNGLADD"
     gene            3628..3831
                     /locus_tag="VBD11_000314"
     CDS             3628..3831
                     /locus_tag="VBD11_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953293.1"
                     /translation="MTENKKRKGYSTVEKQIEADRRYREKNKEKVRIRSYFRTARSFI
                     RNHATNDDLEELTNEINKRREEL"
     gene            3999..4328
                     /locus_tag="VBD11_000315"
     CDS             3999..4328
                     /locus_tag="VBD11_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953294.1"
                     /translation="MKIKTTAKKIIKKVENSTSGLPQKILIMEALIEVEKDGVSQMFR
                     LREFNDEAKKTHIEIMKSVTRNQKYDQKTQENLDNRGRKFTISDLTVDEYRELNALAN
                     EVIDHFK"
     gene            complement(4368..5702)
                     /locus_tag="VBD11_000316"
     CDS             complement(4368..5702)
                     /locus_tag="VBD11_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6953295.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSFDAQCWDGYADYCGFLGVPYSNC
                     TNTGYARDIWEQRHENGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGGG
                     YGWFLGQNQGGANGAYNLVKIPYSATYPTAFRPKVFKNAVTVTGNTGLNKGDYFIDVS
                     AYQQADLTTTCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSSL
                     AQREADLFLSNLPSKKVSYLVIDYEDSASSDKQANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIITKYPNSIWIAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGIAG
                     GLDKNIVLLADDNSQVTVPKADKPQEAVSFNQRLDVNTKLDNSNTPYYEATLSTDYYV
                     ESKPNASSTDKEFIKAGTRVRVYEKINGWSRINAPQSDQWVEDKYLTNATQT"
     gene            complement(5828..6055)
                     /locus_tag="VBD11_000317"
     CDS             complement(5828..6055)
                     /locus_tag="VBD11_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6953296.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALTYHEPKK"
     gene            complement(6048..6350)
                     /locus_tag="VBD11_000318"
     CDS             complement(6048..6350)
                     /locus_tag="VBD11_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953297.1"
                     /translation="MNIDILQIGAASGAILSVVGLWAFVVNPFKTAMQKNEDTMSALK
                     DTIKELAYELKDSQRDREKIHKILDIHEQRLGKTEDDIIVNKEQIKTLFNRRNKYD"
     gene            complement(6510..6902)
                     /locus_tag="VBD11_000319"
     CDS             complement(6510..6902)
                     /locus_tag="VBD11_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6953298.1"
                     /translation="MLDFLNRYPVLLEDKSVKETKAILSFTSSTIKANFEVTLPAEEN
                     DKKFAETLKTCEKLIFEQLYKDKAEAEQFEKINDAIAKSKAQSDKAENMIKLMSATVN
                     DLIKTMADGGKLNDTTLNNASENSSTHI"
     gene            complement(6914..8917)
                     /locus_tag="VBD11_000320"
     CDS             complement(6914..8917)
                     /locus_tag="VBD11_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953299.1"
                     /translation="MTTYYSNSDRSYRLTYIVDEVSTSIADNSSQVRFRLYLTSRTNS
                     YAQYNFSGYAWVGGRYDFSAPSALGFNSNYLLIDKTIKVPHDADGSKTVVVAAKLSGP
                     GGYAPGTLNIPDQKFTLTKIPRTSTVAVSSGYFGDTLNVNINKTNDSFTYDVRYNVNG
                     ITGTVASDISGSTTFKTSLDWASTIPNTTSTPATIYVDTKSNGSVIGTSTGIFYLTVP
                     DNIKPKISSLTLSDTNQKASTIVGANNFVQIISNPVVTFNGASGAYGSTIQNFNAEII
                     GKNQSTQSNGGSLGILNFSGKATVKATVTDSRGRVSDPVTTEINVIPYSPPAFSFTVT
                     RAGAKNDQLVVTRNAKISPLIVDGVQKNKMTLTFKTAPLNTTSFTIDTSNASGTYTTV
                     AELINSTATLSGSYGANKSFDVYGLLSDIFSASGGGTPVKQTVSTESFPLSWHKNSVG
                     IGTLPKIDDTGSLNVAGNIYSDGKQIQQKQLALNNGGSFRHDTTDLNSLQDTGFYCVF
                     KGDNRPSGAGPGYLTVVRHETANYAYQQFYDRTNKTIFTRVLENGAWSGWSEYAKKDS
                     LPQATPAIEDTGWQSIGNGFNYRKIGSMVTIKYDFATNGINSFTVGSMPTNLIPNEMM
                     FAVTAWTVQLNVLNIQVSADGRILWFNPSKWAVNVKGQINWII"
     gene            complement(8930..12751)
                     /locus_tag="VBD11_000321"
     CDS             complement(8930..12751)
                     /locus_tag="VBD11_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6953300.1"
                     /translation="MVIITLVIHDSKLHPVLLLDNEKQGTLNYFDDTWTRQLTTGSSV
                     FEFSVYKKTLEGDNPLNHKYQVLNDQAFVSFVHKDKVQLFNIMQIEETETTVRCYCEN
                     LNLELLNEYCNPYKATKAMSFEEYLVAFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARLLSIANNFDAEIEFETQLHNNYTFKAFIINIYKEYEEGKSYGVGRDRSDTVLRYQ
                     KNIAGITKKLDKRQIYNAIRPYGKKTVKGERVVSNPVTRKVTKTVGSNKTYLGGDIKY
                     YGHTIKKANVQAIINYAVQYNILPSGIITQLYLESFWGDSTVGRRDNNWSGMTGGAQT
                     RSSGVKVTTGMARPANEGGTYMHYASVDDFLKDYTYLLAKQGIYNVVGKKNIADYTKG
                     LFRAGGAKYDYAAAGYQSYTNLMSNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTIQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDISIGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLERSPNAGNLQAGGIYNVKANFGAPFYTGQWGHTGIIKSVSS
                     TRVTVLEQNFAGRMYVVENSYEINAFSRGLQTVCYPREIAQGMAVNGATTQQVSGGTQ
                     ISYEEVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEIETDSQDVLMSTGLKDLKAHAYPAITYEVDGYVDLEIGDVV
                     RIQDDGYQPPLILTARVIEQVLSKDNPNSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYEIKLATSNGVAFKNGTGESVLTPSLQKNGKDYEAVYFYKNGDSLIDIGPSLIVK
                     ASDFNHVLNVTVEAYLNEELVASTQISFTDTEDGADGNTPYWHVAYANSADGTVDFSV
                     SDSANKRYIGQYTDYDAIGSNDPKKYKWTDMVGTVVVGTNNLIDGTKSFSGEDWFTSA
                     TLEDENLSNCPFTLKKWISGQKVSHAKDIMVEQGVTYTFSAYVKREVAGNLYFYLYDI
                     ADGFITSDTPRETIIKNVDSSLRRFEITFTPTKTGRIRPRFAMVSSEQGSFSSGGFML
                     VRGNKTGDWQESEADKASNLDSKADEAFTVEQLNALAERARIAEAELQSKATLDTVND
                     WVKALQDEIKAREGGQKLSEQKLIDFSNRMIAVQQTIGEMQIRTDFVNKFMSQSEDGL
                     VIGQKDGTSSVRVDNDRISFYSSGKEVAYIAQSVLVIDSGIFTTKLQIGRYRIEQYEL
                     NPDINVVRYVG"
     gene            complement(12742..14304)
                     /locus_tag="VBD11_000322"
     CDS             complement(12742..14304)
                     /locus_tag="VBD11_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6953301.1"
                     /translation="MANFSYKGVDLSPFLNCLNIVRTIGNNRSIATRKINELGEAIQS
                     VSFGAKTIFVTVSFKTKEIGASKFVDTTEPSTYSYENLNKLREKIAGILHSKTTFKLT
                     LPDEPDRYYMAVPKGDIDLKGISDWYDETVIEFYIPDGVAHSTTYKKFLDYTRDGNKL
                     TFKLQNEGNTNALPIIKIKHNSENGYIGLANETGAFALGSSEEEDGTVVHRNEVLFDY
                     SKAIAKALEGAPNVAKLNYMPPTFDSELKRMRLDNILGSGKGGEYVAIGNRGTTPGYT
                     EHVGTRTFTINPDSNGEYTFNEHLWWQQIFIATAQDQKGFLKLCVTGINDEFLYGIET
                     YKRKNGFETEYNFFALDDDGVGWRFYKQFEFQADRNYHNPFSMNRSRAVEIFREEDKF
                     RIYFNGAHHHVTVPSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNN
                     IVNKYQPGDEVVINFENDTVKTKDLDSIQDMVLGSTPIFIPPGKTELVMQLSKFSQSA
                     PNVEILMEERWL"
     gene            complement(14305..16953)
                     /locus_tag="VBD11_000323"
     CDS             complement(14305..16953)
                     /locus_tag="VBD11_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6953302.1"
                     /translation="MSNRSYTVQAILKATDTNFTSTMNKVQSAAQATIDKIKSIKDSN
                     ISTLGKIGEYTTMVGQGMQSVGRSLSKYVTLPIVGLGVAAAKTFGDFESQMNRVKAIS
                     GATGADFEKLRKQAIDLGASSVFSAKEVAQGQEMMASAGFNANQILAASPGVMSLAAA
                     SGGDLALASEAAATAVNMFSLNASEATHVADVFAKAAADTNSEVADMAEALKYAGPVA
                     GAMGISMEETAAAIGIMSNAGIKGSQAGTTLRTAITRLAKPTDQMQAVIDGLGLSFFD
                     TNGKMRSLTEITGQLREKMSGLTDQQKSAALSILFGKESLSGMLALINATPGELSKLT
                     EGLKNSKGAADKMANTMNSGLKGTIEQLKGSLETAGITIGGILNPLLQGVIGKIQAVI
                     DWFNKLSPAGQKLAVIIGGIGAALGPLLVIFGTVIIFIGQVTASLAGVSTAFTAIAGI
                     FSIISGPVILVVAAIGAFIAALVVAWNTSETFRNTIISGWKSMVDAVTPLIETLKQLI
                     LSIFTFIQANSATFIGGLKLAWSALVEALSGLFLVITGVVQVGMSVINTTIKVILAII
                     NGDWNAAWTAIKSGVGAIWEGIKNIIIGGLTFLASLFVSMLGVFVSVFSAGWSAIKGI
                     FSVTLKFLVNVVAAGMSAIGNEISSKINTAKSVAILAFNAMKNGISTAINAAKEVVSS
                     VVNRIKNILNSLANINLGAAGRAIMDGFLNGLTSAFEKVKNFVGGIAGWIQEHKGPIS
                     YDRTLLIPAGRAIMGGFNKSLTDSFEPVKKNVSSMASRISEEFQNGLNKLKDINISEI
                     TEGLQSSFDVTHYASFGGLNDSARKLEDDRFVALKDAVLAIRDFANRDVVVTVNGKEL
                     ARTAGDNFTEYQKQKEIMNNRMEGLI"
     gene            complement(16946..17221)
                     /locus_tag="VBD11_000324"
     CDS             complement(16946..17221)
                     /locus_tag="VBD11_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953303.1"
                     /translation="MTVREYNLRMKGYLIKHLETERNIYLSAFIKRNAEATNQSGEYI
                     YKDFKDFYDYDERKRKLLGDKPKQLDKTIVNRAKRVKELREKGGINV"
     gene            complement(17341..17694)
                     /locus_tag="VBD11_000325"
     CDS             complement(17341..17694)
                     /locus_tag="VBD11_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953304.1"
                     /translation="MKEITIAGKTYPLNFGFDFIREMDKRHFVENNGFKFGTGIQTAT
                     LQLSIKNPLILEDIILSATHTLNSIPSKEEIEKWAIKQAEDNKLEEVFEGFLTSLKKA
                     PLSKAQVKQLLKSMN"
     gene            complement(17769..18296)
                     /locus_tag="VBD11_000326"
     CDS             complement(17769..18296)
                     /locus_tag="VBD11_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6953305.1"
                     /translation="MVTSSPVHGKDNILMFRVLGEKKAAAKLSFQTEHKWKFSRKTDA
                     KITKDGAINSDKGLEVTLEIKGVASRDELNTTLKNAVLDGKQIEVWDIDLNSNNNSDN
                     KYDAEYAIGRLGSWEVPSNVEELSEISTEMAIDGKPVKGKATLTKEQIKAIQYVFKDV
                     TETNGDTVSHVEASS"
     gene            complement(18305..18694)
                     /locus_tag="VBD11_000327"
     CDS             complement(18305..18694)
                     /locus_tag="VBD11_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEO6953306.1"
                     /translation="MMKQPDQQLFDEIFKRLTDIGLTVYDFLPPLGTKYPFVVMGDTH
                     IMPRATKTQLIGKCSTTINVWGDGMDRKLISDIVARIMQAVSEINQIENRRWSMIIDD
                     SDTEILKDNSTNENLYRGILNMYFSFI"
     gene            complement(18691..19071)
                     /locus_tag="VBD11_000328"
     CDS             complement(18691..19071)
                     /locus_tag="VBD11_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953307.1"
                     /translation="MINLTLEGEHELLSALKKEVTFENKRKAVRKHGTKMHSKAIDKA
                     VFNGHYEWQKGKGRVFVKPTGATRRSIKLEFSNQSTVAKVKAGTNYSGYLETGTRLME
                     EQPFMKPALDSVIDNFIKDLARVE"
     gene            complement(19064..19363)
                     /locus_tag="VBD11_000329"
     CDS             complement(19064..19363)
                     /locus_tag="VBD11_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953308.1"
                     /translation="MRYNDRVTLLIKANGEPRYDPTLGKRVGGEVKKDVVPCNISEQG
                     IELSNLLDEKLDLDRRIVRLRHSVKKVDRVLIKDKAYRVVTSRNKALYVEEIVND"
     gene            complement(19360..19713)
                     /locus_tag="VBD11_000330"
     CDS             complement(19360..19713)
                     /locus_tag="VBD11_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6953309.1"
                     /translation="MEKINTQTIINNVKLDLDINDTLQDKLLEMLLKRITDHFSAEYG
                     TNEIDSAFSFVLEDCLIARYNRRGAERAKSESVEGRAITYYDFLNEFEPYDLMIKSKL
                     NISNQKSKKGGLYFL"
     gene            complement(19729..19983)
                     /locus_tag="VBD11_000331"
     CDS             complement(19729..19983)
                     /locus_tag="VBD11_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953310.1"
                     /translation="MALVIEAFRDKETGFIYKVGEEYNGARVEFLTGKGVLKATDTQS
                     TNFSNLKVDELKRELDELGVNYDSKAKKAELLELLESHTD"
     gene            complement(19996..21048)
                     /locus_tag="VBD11_000332"
     CDS             complement(19996..21048)
                     /locus_tag="VBD11_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEO6953311.1"
                     /translation="MALIYDVVTSANIKGFYDKQQANVDLSLGEKAFPSKQQLGLKLS
                     FIKGAAGKPVSIKAAAFDTKVPLRDRMSVDLLDEEMPYFKEGMFVKEADRQQLNVLAQ
                     TKNQELIDTVLSTIFNDETTLIAGAKARLEAMRMEVLSSGKIHINSNGVMKDIDYGLA
                     VTQTTKSSQEWSNKETANPLADIEKAIETVTERGHVPEAIVLNSKTFGYIKNAKATVK
                     VIKPLAPEGSIVTKAELKSYLSEELGLNILLKDGVFVNDAGESKKYFPDGVVTLVPNG
                     NLGYTVFGTTPEQSDLMGGQATDAQVSLVETGIAVTTTKTTDPVNVQTKVSMIALPSF
                     ERLDEVQIVTSSEVSL"
     gene            complement(21048..21431)
                     /locus_tag="VBD11_000333"
     CDS             complement(21048..21431)
                     /locus_tag="VBD11_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEO6953312.1"
                     /translation="MNKRTVKTSKEILHNLPYEAISVTLDASKFGNGLASAGTVLAGI
                     DESVFKDRTKKVKPVQNSEVDGEAHIDGILLTDVDLSKGNAAGPLVYRGTVNSDKLAD
                     SALAKDPSKLATKLPHIVFVKGGTK"
     gene            complement(21441..22019)
                     /locus_tag="VBD11_000334"
     CDS             complement(21441..22019)
                     /locus_tag="VBD11_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6953313.1"
                     /translation="MADLVNNGVVDEATQEEVETQEEVKAEATSEKTFTQAEVTEMIQ
                     QNVNRAVAKAHKDAQEQFKAEQDEAKKLAKMNSEEKANYETQKLLEELQQLKDDKTRN
                     ELTAVARKMFSEADINVDDDVLSLAVTLDAEQTKANVTKLASAFAKAIADDRKSLARQ
                     ATPSIGSGSITTQSNFGASLAERAGKVNTKLF"
     gene            complement(22328..23299)
                     /locus_tag="VBD11_000335"
     CDS             complement(22328..23299)
                     /locus_tag="VBD11_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO6953314.1"
                     /translation="MDMHTKEGKSYWRKRVKKEMEAKDKKDIKLGKSMKQIHDYHFRE
                     IEKEIESFYQRYADKEAIDLKLAKKAVSDVDINAYQKKAKELVARANEMRKEGIKVTK
                     ANFTHQENIDMAVYNLKMKVNALELLQLNIDLAMQSLSEDEYKATKKFLEDGFEEELK
                     FQSGLLGTSVSSQNDIKKLAKVTINTNFKGATWSENIWQRQDDLRKIVKEEVYKAITK
                     GDNAIKLSNKLKKEFGVSDSYARRLAITEHARVQMEVSKMSIEDNGFNGFEILPEPSA
                     CSICKGIASDGPYPMEKWDTGNTAPPFHPHCRCAVIGEDIEHKKGKK"
     gene            complement(23283..24776)
                     /locus_tag="VBD11_000336"
     CDS             complement(23283..24776)
                     /locus_tag="VBD11_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6953315.1"
                     /translation="MAHIEDFIDSTGEHKLLELRFHRESRMRYQISDISALFDDNYKI
                     LLEYLKHHENIQKPRIQELLDYAEGNNHEVSKSGRRQDEDMADVRAIHNYGKYISTFK
                     QGYLVGNPIRVEYDDEVNNELLKELAKKNNFHQLNRQLIKDLSKVGRAYELAYRSADN
                     KTKVIRLDPRETFIIYKNDVDKDSLIGVRYYNKSQIDKTDKTVEVYTSSEVIFFEFDG
                     ELTEIGRQPHAFGAVPITEYLNTDDGLGDYETELSLIDLYDSAQSDTANYMQDLSDAI
                     LAIIGRASFPEYVDTPEKAITYLRAMRKARLLNLEPPVDSEGREGSVDAKYLYKKYDV
                     QGTEAYKNRIAEDIHRFTNTPDMTDNKFAGNQSGEALKWKVFGLDQERVDLQALFEQS
                     LKRRYKLIAHVGEFLSEIKDFDISKLKIIFTPNLPKSDQEKINDFKALGGELSNKTKM
                     FVTGIVDDPDEEEDKIMQESQSGSLLAQKLEAQTRMSDKELAHGHAH"
     gene            complement(24789..26030)
                     /locus_tag="VBD11_000337"
     CDS             complement(24789..26030)
                     /locus_tag="VBD11_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6953316.1"
                     /translation="MELSKLYTRKQLQVLDYIWNNDWFICGLHGAKRAGKTVVNNDTF
                     VTELYRVRKIADDLKIDEPIYILAGTSSTSIQNNVLQELYNKYGFEPKYDKHGSFTFC
                     GVKVVQVYTGSISGLKRARGFTSFGAYVNEASLANEVVFKEIISRCSGDGARIVWDSN
                     PDNPNHWLNTDYIGKNKDGIIDFSFKLDDNTFLSKRYIESIKSATPSGKFYDRDILGK
                     WTVAEGAIYSDYDKAVHEVEELPKMTRFFAGVDWGYDHYGSIVIIGEDNNGNYYLVDG
                     IAEQYKEIDWWVTRAKEFIQIYGNVTFWADSARPEHVARFKRERIKARNGRKEVVAGI
                     EYIAKSFKDKRLFIKRGCIPRFFDEIYQYKWKPNSTKDEPQKEYDDVLDAIRYALYSQ
                     HKEDSISKTNNFNVLYQGLKN"
     gene            complement(26017..26514)
                     /locus_tag="VBD11_000338"
     CDS             complement(26017..26514)
                     /locus_tag="VBD11_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6953317.1"
                     /translation="MGKLTLKQQKFIDEYIICGNATDAAIKAGYSKKTAGQIGEQNLK
                     KLEIKQAIQSRMKVLEKSSIATADEVLRVFTQILRQELTEEVTELNQITGEFVTIEKQ
                     PSIAEVIKAGSELMKRYPTNLELKKINLEIEKLKSQIGGDEGQDEKIANFLNMVKGAI
                     SDGVE"
     gene            complement(26749..26836)
                     /locus_tag="VBD11_000339"
     tRNA            complement(26749..26836)
                     /locus_tag="VBD11_000339"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26800..26802),aa:Ser,seq:gct)
     gene            complement(27733..28152)
                     /locus_tag="VBD11_000340"
     CDS             complement(27733..28152)
                     /locus_tag="VBD11_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6953318.1"
                     /translation="MSRNQTKAELMLKRLKGLKLSIRELEKEMNCSNILITSSQFSDM
                     KVSGGIRQSQEDKNIAIISQNEWYLDKISELQKEKTKLLDNIYKLDDIGQINVLIAAY
                     LTYDTFEEAWDGLNISGSTFYSIKRKAIIKLNEVISK"
     gene            complement(28548..28814)
                     /locus_tag="VBD11_000341"
     CDS             complement(28548..28814)
                     /locus_tag="VBD11_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953319.1"
                     /translation="MKEKTIFISKKYANDFNNDKYNLSSGYYFRSGEKHDIAIVKYGE
                     KDYLKNTDLAYVVCDKIVDEGYVGFVYHGEYETWHFKLLNTEAN"
     gene            complement(28811..29320)
                     /locus_tag="VBD11_000342"
     CDS             complement(28811..29320)
                     /locus_tag="VBD11_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO6953320.1"
                     /translation="MNIEEAKKIVDKLSVDSDELWKIPMIPAHKVKALLDTLDQPKPE
                     VPQMIFDVIKSFDDDVDYLHQHMSRQSDEVREWLTHNEREFYEAWLAYPNITIEKEKL
                     YTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKTDNDLQLTESEIRKDFDWA
                     WQFREEVVE"
     gene            complement(29335..29499)
                     /locus_tag="VBD11_000343"
     CDS             complement(29335..29499)
                     /locus_tag="VBD11_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953321.1"
                     /translation="MKVKDLHKVLAKVDPELEVWINEKDEGVLTKAKNVMTWNKHPDK
                     FFINAETYPE"
     gene            complement(29489..29653)
                     /locus_tag="VBD11_000344"
     CDS             complement(29489..29653)
                     /locus_tag="VBD11_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953322.1"
                     /translation="MENYLCYWEKRGQNWYFLANCEDSPTGRIFNLTGVKFNYCPFCG
                     RRLKEVTNES"
     gene            complement(29655..29978)
                     /locus_tag="VBD11_000345"
     CDS             complement(29655..29978)
                     /locus_tag="VBD11_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953323.1"
                     /translation="MSLIDEVKECGSESHKKWFDRFFDDLKIEEKLKQSAMKGFSGYK
                     IAIPKDDEYLARRLDSQKTIDLLKQRLGDGFDVEITNLESSYKIFGKPVVLSRDLCIY
                     WTNGG"
     gene            complement(29981..30466)
                     /locus_tag="VBD11_000346"
     CDS             complement(29981..30466)
                     /locus_tag="VBD11_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6953324.1"
                     /translation="MSDIKILDACCGSRLFWFDKNEEHTTFMDIRQETFDIHGKHVNV
                     KPDVLGDFRNMPFENNIFNLVVFDPPHLKYVGQKSIMKAQYGQLDKDNWKEDISKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKEVLSAIPFTPLFGQRRGTTHWMTFVKFAELTGNG
                     G"
     gene            complement(30491..30568)
                     /locus_tag="VBD11_000347"
                     /pseudo
     CDS             complement(30491..30568)
                     /locus_tag="VBD11_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(30590..30997)
                     /locus_tag="VBD11_000348"
     CDS             complement(30590..30997)
                     /locus_tag="VBD11_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953325.1"
                     /translation="MIPKFRVWDKVTQAMGVVEAIDYVDNKIYTFYRKVVRRYIPFEE
                     AVLMQSTGLKDKNGIEVFDGDIVKCCRLFNDSLSEYVGQVKFVDFGWNIVDKADTHDP
                     FYNYKDGCPDEIREIENIGNIHENPELLERLEG"
     gene            complement(30981..31268)
                     /locus_tag="VBD11_000349"
     CDS             complement(30981..31268)
                     /locus_tag="VBD11_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO6953326.1"
                     /translation="MTDKINAESMQAAYNENYQMFLAKNADYGNSFEKSLNDFGFIAG
                     VVRISDKYNRLYNLINSDKNVSESLSDTLNDMANYCVMLAVWLEVKDDTKI"
     gene            complement(31280..31627)
                     /locus_tag="VBD11_000350"
     CDS             complement(31280..31627)
                     /locus_tag="VBD11_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953327.1"
                     /translation="MTDELINKFYKIFDNGIVRQIKKLDVDCKKAEQIRCSVTNNRRR
                     KTLPRPYVIEAFKDYFDEDTYAQMYIKSYREYHNPNDHETELFVKLKRAHKDTKLEHC
                     KQTKRLIYAAMSF"
     gene            complement(31617..32093)
                     /locus_tag="VBD11_000351"
     CDS             complement(31617..32093)
                     /locus_tag="VBD11_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO6953328.1"
                     /translation="MTFKTEFEIPIEPKPQTRPKFSKFGTYEDPKMKRWRKEVSGWIE
                     KNYDGPFFDDCIKVEVTFYMKAHKTLSKEPTQRSKGKTIQIYQNFVRELIWHAKKPDI
                     DNLIKAVFDSISDAGYDRIQKSGIVWSDDNIVCDLRAKKKYSQNPRIKVRIEEIDR"
     gene            complement(32083..32280)
                     /locus_tag="VBD11_000352"
     CDS             complement(32083..32280)
                     /locus_tag="VBD11_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953329.1"
                     /translation="MFKKQRIYAIYDDDKFVDVGTKEELSARLGIKKQTIEQYMTKSY
                     QALASSKRIALLVEIEEEYDF"
     gene            complement(32441..33238)
                     /locus_tag="VBD11_000353"
     CDS             complement(32441..33238)
                     /locus_tag="VBD11_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HEO6953330.1"
                     /translation="MTKLDLLGKDYYSNESSIKYWSISQYKRFRECETRALAELRGDC
                     TDTRDNTALLVGNYVHSYFESEKAHEEFKAQNGSEMISSRGATKGQLKKDYLVAEQMI
                     EALKNDYQFMKYYQGKKEVAITGFLGEVEFKGKIDCLNVECGYFVDIKTTKGPIDDTV
                     WNGQERVYWFEAYGYILQMAAYKIMLEAKYKKPFKPIIYAVTKETPPDTRAIAIENLD
                     AMQNELDNLAQNIKHLDAVKKGIEPPKPCGHCEYCRANQLTQRVMIF"
     gene            complement(33235..34215)
                     /locus_tag="VBD11_000354"
     CDS             complement(33235..34215)
                     /locus_tag="VBD11_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEO6953331.1"
                     /translation="MASEIVTIQKDITDIVNSKISSLQDEGLVIAPNYAPANALKSAF
                     FKILKTKDRNKRPALEVCTKDSIANALLEMVTQGLSPAKSQCYFIVYGNELQLQRSYF
                     GTMAVLLQQQKIKSIKAEVIYEGDEFELEIVDGEKKFKKHSTHWKNQDNPIEGAYCVI
                     EDVDGNKKLTLMTKKEIDKSWSKTKTGGGTQKEFPQEMAKKTVINRAAKFFINISDDS
                     DILADSINKTTADEFVDERQVKDVTPPETNSLDDLIGTQNEKIDAPSNLKDITDDLHD
                     EEEKPLTDENKTVLEDTSYPADEIPDFDQETGEIKASEGNLFDNLGDLMP"
     gene            complement(34218..34547)
                     /locus_tag="VBD11_000355"
     CDS             complement(34218..34547)
                     /locus_tag="VBD11_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953332.1"
                     /translation="MNTKEKIVVLRNTEDGSFLKSFKNKKDVLAYNVELTDSIHLASF
                     LPEEAYNMQKEKIDNLAETLGCDVVVIEASYDLKFIDGEDVPELTKEQKIKSIVNGMF
                     EQVFGGE"
     gene            complement(34549..34710)
                     /locus_tag="VBD11_000356"
     CDS             complement(34549..34710)
                     /locus_tag="VBD11_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953333.1"
                     /translation="MNEEIYESSRYWQSRYSDLMSDYLKEAEENIELKKQLKSLKAEN
                     WQLKHRKRK"
     gene            complement(34712..34966)
                     /locus_tag="VBD11_000357"
     CDS             complement(34712..34966)
                     /locus_tag="VBD11_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953334.1"
                     /translation="MVEIRINGERVTFDGNFRDAFIFTIDYLRDSEEPTLRQTYNEFK
                     DYTDEDLMEYISTEFDVDPEMFVDTNSDSKWKIKQRILED"
     gene            complement(34953..35228)
                     /locus_tag="VBD11_000358"
     CDS             complement(34953..35228)
                     /locus_tag="VBD11_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953335.1"
                     /translation="MENRPDLKLVAELENRIKDLKIENEILKSKNIDLSEDVKHLKDE
                     YREKDGSMADIMINNKNLRKENNELRAMFDFIKDRLEKFAGSYHGRN"
     gene            complement(35355..36137)
                     /locus_tag="VBD11_000359"
     CDS             complement(35355..36137)
                     /locus_tag="VBD11_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6953336.1"
                     /translation="MILGDENALDKIALSYQKNTKKEETICDKHDCSYITILKTGLTV
                     CPKCHKEDLENQNDLHVQKQYERELENKRLYYLKRLSIMDSELENASFDNFRTDTIKH
                     KEVLVWAKTMANDWFKGGQGNIIMTGKAGRGKSHLAYSIIRGLSDKTKKLGLLVNVTD
                     LLSEIKRDFSKEAFWLDKLKEVDYLVLDDLGAEKVSDWSTSIIYSLLNKRTNTIITTN
                     LTPAEIRQAYGERIASRIRKGCDKSHIMEFEGMEDERMKLWN"
     gene            complement(36124..>36479)
                     /locus_tag="VBD11_000360"
     CDS             complement(36124..>36479)
                     /locus_tag="VBD11_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6953337.1"
                     /translation="INTNNINTNISILNNQNLVPSNQTTTNYIYSIAEQEFGRLLSPM
                     EIETIRTMIKENNHDLIKEAIKRTKLQGKTNLNYVRGILRNWRDDNITTIEQIEAKEK
                     SRKSKQEEVSEYDTW"
BASE COUNT        10393 a         7396 c         5979 g        12711 t
ORIGIN      
        1 tggagtaaat caccatatag tgaaagtttc tattcttcta aagatataga ttggggatat
       61 aaaccagaag gaagcctacg tgtgtctgac cattgggatt ttggcgacga agaggaacat
      121 tgtcctactg tcgaaaaagt acaaggttgg gctgtttgta agtttgaaaa tggtatttac
      181 catcttgtag aaaaattttg aggtgtgaaa tgagttggaa aaaaattcac tttaattcta
      241 tgaatattat ttatgagaac aaaaaaaatt gtctcattaa gatgcctaac aattcaaatt
      301 acaaaaatta taaattttgg catccatcaa aattggtaag gactttagaa aggggaaatg
      361 gttattttaa aacatttagc tttacagatg aatgggaatt taatattttt aaagatgata
      421 aagattacaa taaaactaaa gagattaccc tatctagtga agatatggaa ttagaatttg
      481 aaacgacaaa catagaggtt gaacataatt ctaattctaa gagtttctac gaagagcatg
      541 agcctgtaaa aatcgaaaaa gaagttgagg tactagatga actcacaagg tgattttgta
      601 ttattgacgg aagaccaaca aaaagctatt gaaaagtttt caaggttaaa agtcggtgca
      661 ttgtttatga aacagggttc gggaaaaaca agagttgcgc tagagcttgt taggacaaca
      721 gatagcgaat ttgtgttatt tatgtgtcct ttttctgtca aaaaaaatct tttagatgag
      781 attaatttgt ggggaataga caaagaattt gaagtaattg gatatgaatc catttcatca
      841 tcagacaaag agtacttacg catattagat aaactaaaaa acgcttacaa gccatttatt
      901 gtagcagatg aatctgtttt tataaagaac gacacaagca aagtccacag aagaatgtta
      961 gagtttaggg atttgtcaga gtatcgattg atactaaatg gtacaccaat cacaagagat
     1021 gaatgggata tctacaatca aatggagttt ttaagctaca agatatttaa catgcacaga
     1081 cacgaatttt taagcacttt ttttaaaaca atcagatata aaaagaaagg ttttaatgta
     1141 agagaattta agaagttgtc tgatgttaac atcgattata tgagaaagtt aatagctcct
     1201 tacatctttg aagcagacct aaaatttgac aagaaagaaa ctgaaaaagg aatatctgta
     1261 gattatagcg atgaatgtta cgaaaaatac caggaaagaa aagaaagtct tctaaacagc
     1321 ttagagtatg gattgtcagt tgtacaacaa tttgctaatc tagctgtagc ttgttttgat
     1381 gataaaaaga gacacgagga aatagctaag catttaaaag ggcaagttat cgttttttgt
     1441 acactattgt cagaagttaa aaacattagc aataaaatcg attgttacgt tattactggt
     1501 gaaactaagc caaaagacag agaaaaaata ctagaagatt ttaaaaacga caacaaacca
     1561 ctactactta cgtttgggac tggagcattt ggtcttaatc tacaattctg caatcgtatt
     1621 gcgtttgcaa gtttaacatt tgactatgca aggattgacc aagcaatgag cagaataaag
     1681 cgtctaggtc agtctagaga cattgaatac acttatttca cttctgattt aggtgtttac
     1741 agaatgataa aagagaacat ttttaaaaaa caaagtctaa aagagttaat aattgataaa
     1801 atagataagg gggaattgaa tgaaaacagt ctatgattta gcaatgagca ggataaaata
     1861 tatttttgat aactttgatc acgtttatat ttctttttcg ggtggtaaag acagcggtgt
     1921 aatgcttaat ctagccttaa agtatttaaa agataacaag ctagataaaa aaataacatt
     1981 gatgcactta gattatgaag ctcaatacga gatgacaact gaatatgtcg aaaaaatgga
     2041 aaaggaattt tcaagctatt taaacgtcta tcatatctgt gtgcccttta aagttacaac
     2101 gtgcacttct atgtttcaaa atttttggag gccttgggag gaaggcaagg aagatatatg
     2161 ggttagagac ctacctgaaa acgctaaaac aaaagaggat tttccgtttt ttaaggaaaa
     2221 tatgtgggat tatgagtttc aagaaaaact atcaactttt atacacgata aagaaaaagc
     2281 tgaaaaaact gctgtactcg ttggaataag gacacaagag agcttgcatc gatggcgagc
     2341 gatacataaa gacagagata cttattacaa taacaagaag tatagtaaga aaatatcaga
     2401 aaatgtctat aatttttatc cgatttacga ctggaaaact gaagatattt gggtagctaa
     2461 tgcaaaattt ggatttgcgt ataataaact ctatgattta tattatcaag caggaatgtc
     2521 aattgaccag atgcgtgttg ctagtccgtt tttgtcagag gggcaagaga ctttaaaact
     2581 ttataaggta atcgaaccac atacttgggg taaacttgta agtcgtgtca atggtgttaa
     2641 tttcacgggt ctatatggtg gtactactgc acttggttgg aaaaagataa caaaaccagc
     2701 acatttatca tggaaagaat acatggaatt tttacttgat acgctaccag aagaaacaaa
     2761 gcaaagctat ctatcaaaac tggaaacgtc aataaaattt tggaaagaaa aaggaggcgt
     2821 gctgtctgac gaagttataa aagagcttga tggactatct attaagtatg agtttggaac
     2881 acacggatat aacactaaca aaaaagctgt aaaacttgat tatcttgacg acttagacat
     2941 aaaagatttt aaagcaattc caacttataa gcgcatgtgt atttgtattt taaaaaatga
     3001 ccacttgtgt aaatatatgg gatttagcca aacaaaaaac gaaatgaaga aaagaaagga
     3061 agcaatagaa aaatatgcaa acattttata gcccggttta taatgtcaaa cctgttccaa
     3121 tcgaaaaaat acaagctaac agctacaatc ctaatagtgt agcatcgcca gagatgaaat
     3181 tattgtacca atcaattaaa caagacggat atacgatgcc aatcgtctgc tattatctaa
     3241 aagatataga taaatatgaa attgtagatg gtttccatcg atacactaca atgcttaaac
     3301 acaaagatat atacgagcgt gagaatggct gtttaccagt aactgtcatt gataaaccat
     3361 tatctgacag aatggcttcc actatcagac acaatagagc aagaggctca cacgatatag
     3421 gattgatgac taatattgtc gctgatttag tggattccgg gatgtcagac gcttggatat
     3481 taaaaaatat cggtatggat gcagatgaat tattgagatt aaaacaagtt agcggacttg
     3541 ctgcactgtt tagcaacaag gaattttcta acggtcttgc agatgattaa tgtattttat
     3601 ataatattat aaaaaaggaa tgtgataatg accgaaaata aaaagcgaaa agggtattct
     3661 actgttgaaa agcaaatcga agcagacaga agatatagag aaaaaaacaa agagaaagta
     3721 agaattagaa gttactttag aaccgcaagg tcttttatta gaaatcatgc aactaatgac
     3781 gatcttgaag aactgacgaa tgaaataaac aagcgtagag aagaattata aagcaagtgt
     3841 taatacttac tttttatgtt tacataagct tttaattgcg ttacaattaa atcacaactt
     3901 tgcgattaac ataattaaaa taaagcgatc aatacgcacg ttcttaaaat taacggtata
     3961 gcagagttta cattattaaa gttatgagga gcaacacaat gaaaattaaa acaacagcaa
     4021 agaaaattat taaaaaagta gaaaactcaa catcaggtct cccgcaaaaa atacttataa
     4081 tggaagcgtt aattgaagta gaaaaagacg gagtgtctca aatgtttaga ttacgagaat
     4141 ttaatgatga agcgaaaaaa actcacattg aaataatgaa atctgtgaca cgcaatcaga
     4201 aatacgacca aaaaacacaa gaaaaccttg acaatcgtgg tagaaaattt acaatatcag
     4261 acttaacagt tgatgagtac agggagttaa acgctctagc taatgaagtt attgatcact
     4321 ttaaataata cacaaaaaac cgccctcaat taagagagcg gttaatttta tgtctgtgtt
     4381 gcgttagtga gatatttgtc ttctacccat tggtctgact gcggtgcatt aatacgtgac
     4441 caaccgttaa ttttttcgta aactctcaca cgagtacctg ctttgataaa ctctttgtct
     4501 gtactgcttg cgtttggttt agattctacg tagtaatctg tgctaagagt tgcttcgtag
     4561 tatggcgtat ttgagttatc tagcttagtg ttaacatcta aacgctgatt aaagctaact
     4621 gcttcttgcg gtttatccgc ttttggtaca gtcacttgac tattgtcgtc agctaataaa
     4681 acgatatttt tatctagccc cccagcgatt ccgacgcttg taaattgcca ccagcgcacg
     4741 ccgtccattg atggaaaata gtcccacagt ggttcagagc gcacctcgta atcgggataa
     4801 ccagctatcc aaatgctgtt tgggtactta gtgataattt gttgataatc aatattatta
     4861 agcgtaaaag gtttgtagct gtaataaaca ggcttatatc cagcgtttgc aattttatcc
     4921 ataaacgcaa taactgcatt agtgttagct tgtttgtcgg aacttgcaga gtcttcgtag
     4981 tcaatgacta agtaagagac ttttttgctt ggtaaattgg acagaaataa atctgcttct
     5041 cgttgcgcta agctgctatc tcctccaaat cgtccaaagt gataataacc aatcgggtca
     5101 ctagtattag cttgttgctg atgcctatca gacagccaag cgagtgactc ggatactttg
     5161 ataatcgttt tagtagtgcc agcttgctga caagtcgttg ttaaatctgc ttgctgataa
     5221 gctgatacat cgataaaata atcaccttta tttaagccag tattacctgt aacagtaact
     5281 gcatttttaa aaacttttgg tctaaatgca gtaggataag tcgcggagta tggaattttt
     5341 acaaggttgt atgcgccatt agcaccgcct tgattttgcc ccaaaaacca gccatatcca
     5401 ccaccagcat cactatcaaa gatagcaaca tgactgtacg gtgtaacacc tgcaactacc
     5461 ataaaaatag cgacatctcc cgcttgcata gtctctactt cgtcaaagta gtttaagata
     5521 ccattttcgt ggcgctgttc ccagatgtct cttgcgtatc ctgtatttgt gcagtttgag
     5581 tacggtacac ctaaaaatcc acagtaatct gcataaccgt cccagcattg tgcatcgaac
     5641 gaaccatcaa tatcataagc gttaccattt gacctgcttt tatattcttg ataagttgcc
     5701 atttactcct cctttccaaa aagtaaataa atcggataac taaaaaaagc aaccactgca
     5761 agcggtatgt acagtattgc tattgctagt accattgcta ttttagtgat tgcacgcatg
     5821 tcccctccta tttttttggc tcgtggtaag tcaatgcttg ttcgctatct gatagccctt
     5881 ttgttgttgg gtctgtcaca actccaagca aaaccaaaag cgttactgct gtgttggcaa
     5941 tatccgcaat atttgatggt agtttaatac ctagttgttg cgctagcaaa aatatagctc
     6001 ctaaaatagc catcaaagtt actttgtttt gtagtcgtaa ttttaaatta atcatattta
     6061 tttctcctat taaataatgt ttttatttgt tccttgttga caatgatgtc gtcttctgtt
     6121 tttccgagtc gttgctcgtg gatatccaag attttatgta tcttttccct gtcacgctgt
     6181 gagtctttta gttcgtaagc cagttctttt attgtgtctt taagggcgct cattgtatct
     6241 tcgttttttt gcatcgctgt tttaaacgga ttaacaacaa acgcccacaa tccaactacc
     6301 gataaaatcg ccccgcttgc tgcgccaatt tgtagtatgt caatgttcat tcattgcctc
     6361 attttccttc tgtaccaacc gtagaaactt caattagttt acgtactcgc tcacgacaaa
     6421 atgctggaac gtcatcaata gtaatccacc ctagttcaat ctgcattgca aagtaattaa
     6481 tcatcattgt tttttctcct tttttgtttt taaatatgtg tactgctatt ttcgctagcg
     6541 ttgttaagcg ttgtatcatt caatttccct ccgtcagcca ttgtcttaat caaatcgtta
     6601 acagttgctg acatcagttt aatcatattt tccgctttat ctgattgcgc ctttgactta
     6661 gcaattgcgt cattaatttt ttcaaattgt tctgcttctg ctttgtcttt gtaaagttgc
     6721 tcaaagataa gcttttcaca cgtttttaaa gtttcagcaa acttcttgtc gttttcttcc
     6781 gctggtagcg tcacttcaaa atttgctttg attgtactag atgtaaaaga taaaatcgct
     6841 ttagtctctt ttacactctt gtcttccagt aaaactggat acctattcaa aaaatctagc
     6901 ataactcctc cttttatatt atccaattga tttgtccttt tacgttgaca gcccactttg
     6961 acgggttaaa ccacaaaatg cgaccgtctg cactaacttg tatatttaat acatttaatt
     7021 gcacagtcca cgcagtaacc gcaaacatca tttcattcgg gattaaattc gtcggcatag
     7081 agcccacagt aaaactattt attccatttg ttgcaaagtc atatttaata gtgaccatac
     7141 tcccgatttt tctgtaatta aaaccgtttc cgatagattg ccaaccagta tcttctattg
     7201 ccggtgtagc ttgtggtagg ctatcttttt tagcgtactc actccaacca ctccatgccc
     7261 cattttctag cactcgtgtg aaaatagttt tattggtgcg gtcataaaat tgttgataag
     7321 cgtaattggc tgtctcgtgt cttacaactg ttaaatagcc cggtccagca ccacttggtc
     7381 tgttatcgcc tttaaaaaca caataaaaac ctgtgtcttg caagctgttt aagtctgttg
     7441 tatcatgcct aaaagaacca ccgttgttta aagcaagttg tttttgttgg atttgcttgc
     7501 catcagaata gatattgcct gcgacattca aagaccctgt gtcatcaatt ttgggtaaag
     7561 ttccaattcc aacgctgttt ttatgccatg acagcgggaa agactccgtt gataccgttt
     7621 gtttaacagg tgtaccgcct ccacttgcgc taaaaatatc acttagcaaa ccgtagacat
     7681 caaatgattt gttagctcca tacgaaccac taagtgtagc tgttgagtta atcaactctg
     7741 cgactgtagt ataagtaccg cttgcgtttg atgtgtctat tgtaaaactc gtggtattaa
     7801 gtggtgctgt tttaaaagtc agcgtcattt tatttttttg gacgccatcg acaatcagcg
     7861 gggagatttt agcgttacga gtgacaacta gttggtcatt tttagcgcct gcgcgtgtta
     7921 cagtaaaact aaaagctggt ggggaatatg gtataacgtt gatttctgtt gtcacagggt
     7981 ctgacaccct cccgcgactg tccgttactg ttgctttaac tgtggcttta ccactaaagt
     8041 ttaaaatgcc taatgaccca ccgtttgact gcgtagattg gtttttacct ataatttcag
     8101 cgttaaaatt ttggattgta gagccataag caccactagc cccattaaat gtgacgactg
     8161 gattagaaat tatctggaca aaattattag cacctacaat tgtagatgct ttttgattcg
     8221 tgtctgataa agtgaggctg gaaattttag gcttgatgtt atcaggtaca gttagataaa
     8281 aaatacctgt tgatgtccca atgaccgacc cattcgattt agtgtcaacg taaattgtag
     8341 ctggtgtact agttgtattt ggaatcgtac tagcccaatc caaactagtt ttaaaagtcg
     8401 ttgatcctga tatatcacta gccacagtac cagttatccc gttaacgtta tatctgacat
     8461 cgtatgtaaa gctgtcattt gttttattga tgttgacatt tagcgtatca ccaaaataac
     8521 cactacttac agcaactgtg cttgttcgtg ggattttagt cagcgtaaat ttttggtctg
     8581 gtatatttag cgttccgggt gcatacccgc caggacctga caacttagct gcgacaacca
     8641 cagtcttact accgtcagca tcgtgtggta ctttgatagt tttatcaatc agtaaataat
     8701 tactgttaaa gccaagcgca gatggggcgc taaagtcata tctaccacca acccaagcat
     8761 atccgctaaa gttgtattgt gcgtaactgt tagtccttga tgttaaataa agtctaaatc
     8821 taacttgact gctattatct gcgattgatg ttgagacctc gtcaacaata taagttaagc
     8881 ggtaactcct atcagagtta ctataataag ttgtcattta acctccttcc tatccaacat
     8941 agcggacaac atttatatct ggatttagct cgtattgctc aatacgatag cgccctattt
     9001 gtagtttggt cgtaaagata ccactatcaa taactaacac actttgagca atataagcga
     9061 cttctttacc acttgagtaa aagcttatac ggtcgttatc aacacgaaca ctagaagtac
     9121 cgtctttttg accaataaca agaccgtcct ctgactgact cataaattta ttaacaaaat
     9181 cagtgcgtat ctgcatctcc ccaattgttt gctgtactgc tatcatgcga ttagaaaagt
     9241 ctatcagttt ttgttctgat aacttttgtc ctccctctcg tgctttgatt tcgtcttgca
     9301 atgctttaac ccagtcgttg actgtgtcta acgttgcttt agattgcaat tcagcttctg
     9361 cgatgcgagc acgttcagcg agtgcattta gttgctcaac tgtaaacgct tcgtcagctt
     9421 ttgaatcaag attacttgct ttatcagctt ctgattcctg ccagtcgcct gttttatttc
     9481 ccctaacgag cataaaccca ccagagctga aactaccttg ctccgatgac accatcgcga
     9541 accttggtct aatcctacct gtcttagttg gtgtaaaagt gatttcaaaa cgtctgagac
     9601 tagagtcaac gttttttata attgtctctc gtggggtatc gctagtaata aaaccatctg
     9661 ctatatcata aagataaaaa tataaattcc cagctacctc acgtttaaca taagcactaa
     9721 aagtgtacgt tacaccttgc tcgaccatga tatcttttgc atgcgacact ttttgcccac
     9781 taatccattt tttaagcgtg aaaggacaat tagagaggtt ctcgtcttct agtgttgcag
     9841 aagtaaacca gtcttctccg ctaaacgact tagtgccatc gattagatta tttgtcccaa
     9901 cgacgaccgt cccaaccata tcagtccact tatatttttt agggtcgttt gagcctattg
     9961 catcgtagtc agtatattgc ccaatgtagc gcttgtttgc gctatcagac acgctaaaat
    10021 caactgtccc atctgcgcta tttgcataag caacatgcca gtaaggcgta ttcccgtctg
    10081 caccgtcttc tgtgtctgta aatgatattt gtgtacttgc cacaagctcc tcatttaaat
    10141 acgcctctac tgttacgttt aaaacgtggt taaagtcgct tgctttaaca attagtgatg
    10201 gtccgatatc aattaatgag tcgccatttt tgtaaaaata aacagcttca tagtcttttc
    10261 cgtttttttg caaacttgga gttaagacag attcaccagt gccattttta aaagcgacac
    10321 cgttcgaagt cgctagttta atttcatacg gaattgactc atcgtataga cgcaacatat
    10381 cgctgattaa atcagacgct agttgacttt ctttctcaac aaaattacta aatttagttt
    10441 tgtttgagtt tggattatct ttagacaaaa cctgctcaat aacacgagca gtcaaaatca
    10501 aaggtggttg ataaccatca tcttgaatgc gtacgacatc gccaatttct aaatcaacat
    10561 agccatcaac ttcataagtg attgctggat aggcgtgtgc ttttaagtct tttagacctg
    10621 ttgacatcaa gacgtcttga ctatcagtct caatttccat gtcctttcgt atccagttat
    10681 cccgtgtctc attacctgtt aaaacagatg gataacggtc tctggataaa ggtgcgtaca
    10741 aaaatccatt tttgagatag tactctactt taccgttttc atctttccac tctttgtaga
    10801 ttgagttgtc aatgtagatg atttgttctt cttcgtatga ttctgtctgt gcttcttgta
    10861 cgacttcctc atatgatatc tgtgtcccac cactaacttg ctgtgttgtt gcaccgttga
    10921 cagccattcc ttgtgcaatc tcacgaggat agcatactgt ttgcaatcct ctagaaaaag
    10981 cgttaatctc atatgagttc tcaacgacat acatacgccc agcgaagttt tgctctaaaa
    11041 cagtgacacg agtgctagac acactcttaa taatccctgt atgtccccat tgacctgtgt
    11101 aaaacggtgc gccaaagttt gctttgacat tgtagatacc gccagcttgc aagttaccag
    11161 cgttaggtga cctctctagc ttccagccat aagcccccca gttataatca gtaccgatta
    11221 aagcagcagc catgccaccg ccaattcgac cacgtatacc cccaatagag atatcaatcc
    11281 aagcgccatc taacttctta gcgtaccagc ctgacaaagc gtaacactgt ccagaaccaa
    11341 tcctacgccc tttaagtcta gtagcttcat ttagtgcttg tattgtctta gtagctcttc
    11401 tagctacgtt tacagctgtt ataggctgta ctggcgtttg ccacagctta tcaatcgtat
    11461 taaggatatt cccggtcact ttattaatac catttcggat attagacatc aaatttgtgt
    11521 agctttgata tcctgctgct gcgtagtcat atttagcgcc accagctcta aaaagccctt
    11581 ttgtatagtc tgctatattc tttttgccga cgacattata aatcccttgt tttgctaaaa
    11641 gataagtgta gtcttttaaa aagtcgtcta cactagcata gtgcatgtac gttccgccct
    11701 cgtttgcagg acgagccata ccagtagtga cttttactcc gctagaacgt gtctgtgctc
    11761 cacctgtcat tcctgaccag ttgttatcac gcctaccgac agttgagtca ccccaaaagc
    11821 tctctaaata aagttgcgtg atgattccac ttggcaaaat gttgtattgt acagcatagt
    11881 ttataatcgc ttgtacgttg gcttttttga ttgtgtgacc gtaatattta atatcgccgc
    11941 ctaagtaagt cttgttagag ccaactgtct tagtgacttt acgtgtaaca ggattagaga
    12001 caacacgctc accttttaca gtctttttac cgtaagggcg tattgcgttg taaatctgac
    12061 gcttatcaag ctttttagta ataccagcga tatttttttg gtatctaagc acagtgtcac
    12121 ttctatcacg gccaacaccg tatgacttgc cttcttcgta ttctttatag atgtttatga
    12181 taaaagcttt aaacgtgtag ttattgtgta gttgcgtttc aaactcaatt tctgcatcaa
    12241 aattattagc aatcgataac aagcgagcca gtttagtgtc ttgaccagtc cattctaaag
    12301 ttagcttctt gtcctttact tcgtttgtgc caattgtcaa agctccccag tttaaaatgt
    12361 caaatgctac aagatactct tcaaacgaca tcgctttagt ggctttgtac gggttgcaat
    12421 actcgtttag caactctaaa tttaagtttt cgcaataaca acgtacagtc gtttcagtct
    12481 cttcgatctg catgatatta aagagctgta ctttatcttt atgtacaaac gatacaaacg
    12541 cttggtcatt aagcacttga tacttatgat tgagtgggtt gtctccttca agtgtttttt
    12601 tgtacacaga aaactcaaaa acagatgagc ctgtagttaa ttgtctagtc catgtgtcat
    12661 caaaataatt aagtgtccct tgcttttcgt tgtctagcaa caaaacagga tgtagcttag
    12721 aatcatgtat tactagcgtt attataacca cctctcttcc atcaatattt caacgtttgg
    12781 cgcagattga gaaaacttag ataactgcat aaccaactct gtttttccgg gcggtataaa
    12841 tataggtgtc gaacctaaaa ccatgtcttg aatggaatct aagtcttttg ttttgactgt
    12901 gtcgttttca aaattgataa ccacttcatc gcctggctga tacttattaa caatattgtt
    12961 gtagtgagac actcccattt tttcaaagtt gaccttttcg aaaaggttat agttgatata
    13021 tttagagctg tcactacatg tccccattgc caagtgtatc ttgcgggatt ttttcccttt
    13081 aagggacgga acagttacat gatgatgcgc accgttaaag taaatacgga acttatcttc
    13141 ttctctgaaa atctcaaccg ctctgcttct attcatcgaa aaaggattgt gataatttct
    13201 atctgcttga aattcaaact gcttgtaaaa tctccagcca acaccgtcat catcaagagc
    13261 aaagaaattg tattctgttt caaaaccatt ttttcgtttg taagtttcga tgccatataa
    13321 aaattcatcg ttgattcccg ttacacaaag tttcaaaaat cccttttggt cttgcgctgt
    13381 tgcaataaaa atctgttgcc accacaagtg ttcattaaac gtgtactcgc cgttagaatc
    13441 ggggtttata gtaaatgtac gtgtcccaac gtgctcggtg tagccgggtg tggtgcctct
    13501 attcccaata gctacatact ctccaccttt gccagagcct aaaatattat caaggcgcat
    13561 gcgctttaat tccgagtcaa atgttggtgg catatagttg agttttgcaa catttggagc
    13621 accttctaaa gcttttgcaa tagcttttga gtaatcaaaa aggacttcgt tacgatgcac
    13681 gacagtcccg tcttcttctt ctgatgatcc aagtgcaaaa gcacctgttt cgtttgcaag
    13741 accaatatag ccattttcag agttatgttt aattttgatt attggcaacg catttgtgtt
    13801 accttcgttt tgcaatttaa aggttagttt atttccatct cgcgtgtaat ctaaaaactt
    13861 tttgtaagta gtcgaatgtg caacgccgtc tggtatgtaa aactcaataa ccgtttcgtc
    13921 gtaccaatca gatattcctt ttaagtcaat atcacctttt ggaacagcca tataatatct
    13981 gtcaggctca tctggtaatg taagtttaaa ggtcgtctta ctgtgcaaaa taccagctat
    14041 tttttctctt aatttattta aattttcgta actataagtc gacggttctg tcgtgtctac
    14101 aaatttactt gctccgattt ctttagtttt aaaactaaca gtaacaaaaa tagttttagc
    14161 tccaaaacta accgattgaa tggcttctcc taattcattt atttttctgg tagcaataga
    14221 cctattatta cctatagtcc tcactatgtt taaacaattc aaaaaaggcg ataaatcaac
    14281 gcctttataa ctaaaatttg ccaattatat caaaccttcc attctattat tcattatttc
    14341 tttttgtttt tggtattctg tgaagttgtc tccagctgtt cgagctaatt ctttaccatt
    14401 cacagtcact acaacgtctc tattagcaaa atctctaata gctaaaaccg catctttcaa
    14461 tgcgacaaat ctatcatctt ctaattttct tgctgagtca tttagtccgc caaaacttgc
    14521 ataatgagtc acgtcaaaac tactttgtaa accttccgta atttccgata tgttgatatc
    14581 tttaagttta ttcaatccgt tttgaaactc ctcagaaata cgactagcca tactagaaac
    14641 atttttcttg actggttcaa agctatctgt aagagattta ttaaaacctc ccattatagc
    14701 tctacccgct ggaataagta atgttctatc gtaacttatt ggccctttat gctcttgaat
    14761 ccatccagcg ataccaccga caaaattttt gactttttca aaagctgagg tcaatccatt
    14821 taaaaatcca tccattatag ctctaccagc tgctcctaag ttaatattag caagggaatt
    14881 aagaatgttt tttatacggt taactacact agaaacgact tctttcgcag cattaatagc
    14941 tgttgaaata ccgtttttca tcgcattaaa tgctagtata gcgacacttt ttgctgtatt
    15001 aattttagat gatatctcgt ttccaatagc actcattcca gcagctacta cgttaaccaa
    15061 aaattttagc gtcacagaaa atataccttt tatagcactc catccagcag aaaaaactga
    15121 tacgaaaaca cccagcatac ttacaaataa actagctaaa aacgtcagac ccccaattat
    15181 tatgtttttt ataccttccc aaattgctcc tactccagat ttgatagcgg tccatgctgc
    15241 attccaatca ccatttataa ttgctaatat cacttttata gttgtgttga tgacagacat
    15301 gccaacttgt accacgcctg ttataaccag aaacaaacca cttagagctt caacaagcgc
    15361 actccaagct aactttaaac cgccaataaa agtagcgctg ttagcttgta tgaaagtgaa
    15421 aattgataat attagttgtt tcaatgtttc gattagtggg gtgactgcat caaccattga
    15481 tttccaacca gaaataattg tatttctaaa tgtctcagat gtgttccaag cgactacaag
    15541 tgcagctata aatgctccga ttgctgcaac aactaaaatt acaggtcctg aaattattga
    15601 aaagattcct gctattgccg tgaacgctgt agaaactcct gctaaagaag cagttacttg
    15661 accaataaaa ataattactg ttccaaaaat gaccaaaaga ggcccaagag ctgcgccaat
    15721 acctccaata ataactgcca gcttttgccc agcaggagat aacttgttga accaatctat
    15781 gactgcttgg attttaccaa taactccttg caacaatgga tttaatatac caccgattgt
    15841 aatgccggct gtttctaaag aacctttgag ctgttcaatt gttcctttaa gaccgctatt
    15901 catagtgttt gccattttgt cagcagcacc ttttgaattt ttcaaccctt cggttaattt
    15961 agataactcg cctggggtag cgttaattaa agcaagcatc cctgacagag actctttacc
    16021 aaataaaatt gataaagctg cagatttttg ttgatctgtc aatccagaca ttttttctct
    16081 tagttgtcct gttatttctg tcaaagaacg catcttccca ttcgtatcaa aaaaagacaa
    16141 tccgagacca tcaataacag cttgcatttg gtctgtcggt tttgctaatc tggtgatagc
    16201 tgttctaaga gttgtaccag cttgagagcc ttttatacca gcgtttgaca taatacctat
    16261 tgctgctgcg gtttcttcca tagaaattcc catagctcct gcaactggtc ctgcgtattt
    16321 taacgcttcc gccatgtctg caacctctga gtttgtatct gctgcagctt ttgcgaacac
    16381 atcggctaca tgagtggctt cacttgcgtt caagctaaac atatttaccg cagtcgctgc
    16441 cgcttctgag gcaagagcta gatctccacc agatgctgca gccaaagaca ttactccggg
    16501 tgaagctgct aagatttgat ttgcgttaaa tcctgctgat gccatcattt cttgaccttg
    16561 tgcaacttcc ttagcactaa aaacagacga tgccccaaga tcaatagctt gttttcttaa
    16621 cttttcaaaa tctgccccgg tagctccaga gattgctttt actctgttca tttgagattc
    16681 aaaatctcca aatgtttttg cagcagcgac tcctaaccca actattggaa gtgtgacgta
    16741 ttttgaaagg ctacgcccaa cactttgcat cccttgacct accatagtcg tatattcgcc
    16801 gattttccca agcgtagata tgttgctatc ttttattgat tttattttat caatagtagc
    16861 ttgtgcagca gattgaactt tgttcatagt gcttgtaaaa ttagtatctg ttgcttttaa
    16921 aatcgcttgt actgtgtacg atcgattaga cattaatacc tcctttctct ctcaactctt
    16981 ttaccctttt agctctatta acaatagttt tatccaattg tttgggttta tcacctaaca
    17041 attttctctt ccgctcatcg taatcataaa aatctttaaa atctttataa atatactcac
    17101 cactttggtt ggtggcttct gcgtttcttt ttataaaagc gctaagatag atatttcttt
    17161 cggtctctaa atgcttgatt aaataccctt tcatcctaag attgtattct ctgacagtca
    17221 tattcctagc gacatcatag tcagttacat tcagtaaccc gaaaatgtta gctattattt
    17281 cgttgtacgt ttctagagaa tttttttcgt tctctttgtt gccgtttgca gactcaattt
    17341 ttagttcatg ctttttagta attgtttcac ttgtgctttc gatagtggcg ctttcttcaa
    17401 actcgttaaa aaaccttcaa aaacctcttc aagcttattg tcttccgctt gtttaattgc
    17461 ccatttttcg atttcttcct tgcttggaat gctattcaaa gtgtgtgttg ctgacaagat
    17521 aatatcttct agaattaggg ggtttttgat agataattgt aatgttgctg tttggatacc
    17581 tgttccaaat ttaaaaccgt tgttttcaac gaaatgcctc ttgtccatct cacggatgaa
    17641 gtcaaaccca aagtttaatg ggtaagtttt tcctgcgatt gtaatttctt tcatttttga
    17701 ttttctctcc ttaaaaaaat aacaagggta aaaacccttg ttttaaaaat taattgcctt
    17761 gtgttagatt aactacttgc ttctacatga gaaacagtat cgccgtttgt ttcagtgacg
    17821 tctttaaaca catattgaat agccttgatt tgctctttgg tgagggtagc tttacctttg
    17881 acaggttttc catcaatagc catttctgtg gaaatttcag ataattcttc aacatttgaa
    17941 gggacttccc acgaacctaa tcgacctatt gcgtactctg catcatattt attgtctgaa
    18001 ttgttgttgc tatttaagtc aatatcccag acctcaatct gcttgccatc taaaacggca
    18061 ttttttaatg ttgtgttcaa ttcgtcgcga ctagcaaccc ctttgatttc taaggtgact
    18121 tctagtcctt tgtcagaatt tatagcacca tcttttgtga tcttagcatc tgtttttcta
    18181 ctaaatttcc acttatgttc cgtctgaaaa gataatttag ccgcagcctt cttttccccc
    18241 aaaacacgaa acatcaaaat attatcttta ccatgaactg gtgaacttgt taccatgctt
    18301 cctcctatat aaaactaaaa tacatgttca aaatgccacg atataaattc tcgttagtac
    18361 tattatcttt taagatttcg gtatcactat catctataat catggaccat cttctatttt
    18421 ctatttggtt tatttcactg accgcctgca taattcgagc aactatatca cttatcaatt
    18481 ttctgtccat gccgtctccc cagacgttta tcgttgttga gcacttgccg attaattggg
    18541 tttttgtcgc tctcggcatt atatgagtgt ctcccatcac aacaaaaggg tattttgtac
    18601 ccaacggtgg aaggaaatcg taaacagtca atcctatatc agtgagtctt ttaaaaatct
    18661 catcaaacaa ttgttgatcc ggttgtttca tcattcaacc ctcgctaaat ctttaatgaa
    18721 gttgtcaatg acactatcaa gagctggctt cataaacggt tgctcctcca ttaatcgtgt
    18781 acctgtttcc aagtaaccgg aataatttgt cccagccttc actttagcaa ctgtactttg
    18841 attactaaac tctaatttaa tagaccttct agtagctcct gtaggtttaa caaaaacacg
    18901 ccctttgcct ttttgccact cataatgccc attaaataca gctttatcaa tagcctttga
    18961 gtgcatttta gttccgtgtt ttctgacagc tttgcgcttg ttttcaaaag taacttcttt
    19021 ttttaacgca gatagcaact catgttcccc ttccaatgtt aagttaatca ttaacaatct
    19081 cctcaacgta taaagcttta tttctgctcg ttacaacacg ataagcttta tctttgatta
    19141 aaaccctatc caccttctta acgctgtgac gtagccttac aatgcgtcta tcaaggtcta
    19201 acttctcgtc taacagatta gatagttcta taccttgctc cgagatgtta cacggaacga
    19261 catctttttt aacttctcct ccaacacgtt tcccaagagt tggatcatat cgaggttcac
    19321 cgttagcttt aattaaaaga gtaactctat cgttatatct cataaaaaat aaagtccacc
    19381 ttttttagat ttttgatttg aaatattaag cttactttta atcattaaat catacggttc
    19441 aaactcgttt aaaaagtcgt aataagtgat agctcttcct tccacggact cagacttagc
    19501 ccgttcagca cctcgcctgt tatagcgagc aatcaaacaa tcttcaagga caaatgaaaa
    19561 ggcactatct atctcattag tgccatactc tgctgaaaag tggtcagtaa tcctttttag
    19621 caacatttcc aatagtttgt cttgtaatgt atcgttgata tctaaatcaa gctttacatt
    19681 attaatgatt gtttgcgtgt ttatcttttc cataaacacc tccaaaaatt aatcggtgtg
    19741 agattctaaa agctctagca attccgcttt tttagcttta gaatcgtaat taacacctag
    19801 ttcatcaagt tcacgcttta gctcgtcaac ttttaaattg ctaaagtttg ttgactgtgt
    19861 gtcagtagct tttaaaacac ctttcccagt caaaaactca actctagcac cgttatactc
    19921 ttcaccgact ttataaataa aaccagtctc tttatctcta aaagcttcaa ttactagagc
    19981 cacgattacc tccttttata atgatacttc cgaacttgtt acaatctgta cttcatctaa
    20041 gcgctcgaat gatggtagag caatcataga aaccttagtc tgtacgttaa caggatcagt
    20101 agtcttagta gttgtaacag caatacctgt ctctacaaga gatacctgtg catctgttgc
    20161 ttgacctccc atgaggtcag actgttcagg agttgtccca aatactgtat agccaagatt
    20221 tccgttaggt acaagtgtaa ctacgccatc aggaaaatac ttcttgcttt cacctgcgtc
    20281 gttaacgaac acaccatctt taagtaagat gtttaatccc aattcttcag aaagataaga
    20341 ttttaattct gctttagtaa caattgagcc ttctggtgca agtggtttaa ttacttttac
    20401 ggttgctttt gcatttttga tatagccaaa agtttttgag tttaagacaa tagcttcagg
    20461 aacgtgacca cgctctgtta ctgtttcaat agctttctca atgtcggcaa gagggttagc
    20521 agtttcttta tttgaccatt cttgtgaact tttagtcgtt tgagttacag ctaatccata
    20581 atcgatatct ttcataacac cgtttgaatt gatgtggatt ttaccacttg acaacacttc
    20641 catgcgcata gcttcaagac gtgctttagc accagcgatt agagtagttt catcattaaa
    20701 gattgttgat aacactgtgt caataagttc ttgattttta gtttgtgcta aaacgttaag
    20761 ttgttgacgg tcagcctctt ttacaaacat accttctttg aagtaaggca tttcttcgtc
    20821 taacaagtct acagacatgc ggtcacgaag tggaacttta gtgtcaaacg ccgccgcttt
    20881 gatactaact ggtttgccag ctgctccttt gataaaggat aacttaagac caagttgttg
    20941 tttagatggg aaagcttttt ctcccaaaga caagtcaacg tttgcttgtt gtttatcata
    21001 aaatcctttg atgttagcag atgttacaac gtcataaatt aatgccatta tttagttcct
    21061 ccttttacaa atacaatgtg tggtaattta gtagctaatt ttgatggatc tttagccaaa
    21121 gcagagtctg ccaacttatc agaatttact gtgccacgat aaacaagagg acccgcagca
    21181 ttgcctttag ataagtcaac atctgtcaac aaaatgccat cgatatgagc ttcaccatct
    21241 acttcactgt tttgaactgg ttttactttt ttagtacgat ctttaaaaac agactcatca
    21301 atccctgcta aaactgttcc agcagatgcc agtccattac caaatttact tgcatctaaa
    21361 gtcacagaaa tcgcttcgta tggcaagtta tgtaaaatct cttttgatgt ttttactgta
    21421 cgtttattca ttttttcctc ctaaaatagt ttggtgttaa cttttccagc tcgttctgct
    21481 aagcttgcac caaaatttga ttgagttgta atagagccac ttccgattga aggggtggct
    21541 tgtcgtgcca atgatttgcg atcatcagcg attgctttag caaatgcgct agctagctta
    21601 gtgacatttg cttttgtttg ctctgcatct aaagttactg caagactaag aacatcatca
    21661 tcaacattaa tatcagcctc tgaaaacatt ttacgagcaa ctgctgttag ttcgttgcgt
    21721 gtcttatcat cttttagttg ttgcaactct tctaacaatt tctgtgtttc gtagttagct
    21781 ttttcttcgc tgttcatctt tgctaatttt ttagcttcgt cttgctctgc tttaaattgc
    21841 tcctgagcgt ctttgtgagc ttttgcaact gcacgattaa cattctgttg tatcatttca
    21901 gttacttccg cttgtgtaaa agtcttctct gatgtagctt ccgctttaac ttcttcttga
    21961 gtttcgactt cctcttgtgt tgcttcgtct actacaccat tgttaactaa atctgccatg
    22021 aggcgcctcc tgtttaaagt catgtctgac tattaaatct tgcacagttt atagccgtaa
    22081 gcacgttttg ggcataataa aaaccagcct caactggatt tgtgactatt tatttttcca
    22141 ttttttcttt gagtgttttt tgatacgact aagctcgttg ttagtcgctt gtgcgttatg
    22201 ctctacaatt ttttcaagtt gttcaatccg ttcgtgttgt ttgcttaatt ctttaaaaaa
    22261 aattcgtttt ctacaataag tgaagctatg tagcgttcga ttttgcgttt tttcttaatg
    22321 cgtttgttca ttttttacct ttcttatgtt cgatatcttc accaataact gcacaacgac
    22381 aatgtgggtg aaatggcggt gctgtattgc ctgtatccca tttttccata ggatacggac
    22441 cgtcacttgc tattccttta caaatagaac aggcggaggg ctctggcaat atctcaaaac
    22501 cattaaaccc gttgtcttct attgacattt tgctaacttc catttgtacc cttgcatgtt
    22561 ctgtgattgc tagacgtcta gcatagctat ctgatacccc aaactctttt tttagcttat
    22621 tagacaactt aatggcgtta tcgccttttg ttatagcttt ataaacttcc tcttttacta
    22681 tttttctaag gtcgtcttgc ctttgccaaa tattttcact ccacgttgcg cctttgaaat
    22741 ttgtatttat ggttacttta gctagctttt taatgtcatt ttgactagaa acagatgttc
    22801 caagtaagcc agactgaaat ttcaactctt cttcaaaacc atcttcaagg aatttttttg
    22861 tcgctttgta ttcatcttcc gataagcttt gcatcgctaa atcaatattt aattgcaaaa
    22921 gctctaaagc gttaactttc atctttaaat tataaacagc catgtctata ttttcttgat
    22981 gcgtaaaatt agctttagtg actttaattc cctctttgcg catttcgtta gcccttgcaa
    23041 ctaattcttt tgcctttttc tgataagcgt tgatatcaac gtcagagaca gcttttttag
    23101 cgagctttaa gtctattgcc tctttatctg cataacgctg gtaaaaagac tcgatttctt
    23161 tttcgatttc acgaaaatgg taatcgtgta tctgtttcat ggactttccc aacttgatat
    23221 cttttttgtc tttggcttcc atctcttttt taacacgttt acgccaataa cttttacctt
    23281 ccttagtgtg catgtccatg agctaactcc ttgtctgaca tacgtgtttg agcttctagt
    23341 ttttgagcta acaaactgcc cgactgcgat tcttgcatga ttttatcttc ttcttcatct
    23401 ggatcatcaa caataccagt gacaaacatc ttagttttgt ttgataactc tccaccaagt
    23461 gctttaaaat catttatttt ttcttggtct gacttaggta agtttggagt aaagattatt
    23521 tttagcttgc tgatatcaaa atctttaatt tcacttaaaa actcaccaac atgagcgata
    23581 agcttatatc tacgtttcaa cgactgctca aataacgctt gtaagtccac acgttcttgg
    23641 tctagtccaa aaactttcca cttgagcgct tctccggact gattaccagc aaatttgttg
    23701 tctgtcatat ctggcgtatt agtaaatctg tggatatcct cagcgattct gtttttatac
    23761 gcttcagttc cttgcacgtc atatttttta tacaaatact tagcgtcaac agaaccttca
    23821 cgtccttcgc tatcaacagg tggttctaga tttaacaatc tagcttttcg catagctctc
    23881 aaatatgtaa tagctttttc cggtgtatct acgtattcag gaaacgacgc acgaccaata
    23941 attgctagaa tagcgtctga caagtcttgc atgtagttag ctgtgtctga ctgcgcagag
    24001 tcgtataaat caatcaaaga taattctgtt tcgtaatcgc ctaaaccgtc atctgtatta
    24061 agatattccg tgatagggac agcaccaaaa gcatgcggtt gtctacctat ctctgttaat
    24121 tctccgtcaa attcaaaaaa gataacttcc gaacttgtat aaacttctac tgtcttgtct
    24181 gttttatcta tttgactttt gttgtagtat cgcacaccaa taagactatc tttatcaaca
    24241 tcatttttat agataataaa agtctctctc gggtctagtc ttatcacttt tgttttattg
    24301 tctgcactac gataagcgag ttcgtaagca cgaccaactt tagacaagtc ttttataagt
    24361 tgtctattta attgatggaa gttgttcttt tttgctaact cttttaaaag ttcgttgtta
    24421 acttcatcgt catactcaac acgtattgga ttaccaacaa gatatccctg tttaaatgtt
    24481 gatatatact tgccataatt atgtatggca cgaacatcag ccatgtcctc atcttgccta
    24541 cgaccagact tagatacctc gtgattgttt ccttctgcat aatctaacaa ctcttgtata
    24601 cgtggttttt gaatgttctc gtggtgtttt aagtattcaa gtaatatttt atagttgtca
    24661 tcaaacaaag cgcttatatc gcttatctga tacctcattc ttgactcacg atgaaaacgc
    24721 aactctaaaa gtttatgttc cccagttgag tcaataaaat cttctatgtg tgccattgtt
    24781 tctcctatct agtttttaag accttgataa agtacattga aattgttggt tttacttatg
    24841 ctatcttctt tatgctgtga ataaagagca tatctgatgg catctagcac atcatcgtat
    24901 tctttttgtg gttcgtcttt tgtgctgtta ggtttccatt tgtattgata tatctcatca
    24961 aaaaaacgag gaatacaccc tcgcttgata aataaccttt tatccttgaa tgatttcgct
    25021 atatattcaa tgcctgcaac aacttcttta cgtccatttc ttgctttaat acgctctctt
    25081 ttaaagcgag caacgtgttc tggtctcgca ctatctgccc agaatgtaac attcccatag
    25141 atttgtataa actctttggc tctagttacc caccaatcta tttctttata ttgctctgca
    25201 ataccatcaa ctaagtaata atttccatta ttgtcctctc cgataatcac aattgaacca
    25261 tagtgatcat atccccagtc gacaccggca aaaaagcgtg tcatttttgg caattcttcg
    25321 acttcgtgaa cagctttgtc ataatctgaa tagatagcgc cctcagcaac tgtccatttg
    25381 cctaaaatgt cacggtcata aaacttccca ctaggcgttg cgctttttat cgactcaatg
    25441 taacgcttag atagaaaagt gttatcatct aacttaaaac taaaatcgat aatgccatct
    25501 ttgtttttac cgatgtagtc agtgtttagc caatgattag gattatctgg gtttgagtcc
    25561 catacaattc tagcaccatc accagaacaa cgagaaatta tttccttaaa cacaacctca
    25621 ttagctagtg atgcctcatt aacataagct ccaaatgatg taaaacctct agctcttttt
    25681 aacccactaa tagaccccgt atatacttgc acaaccttaa cgccacaaaa tgtaaaagat
    25741 ccatgtttgt catatttagg ctcaaagcca tatttattat aaagctcctg taacacgttg
    25801 ttttggatag aagtagatga tgtcccagct aaaatataga taggttcatc tatttttaag
    25861 tcatctgcaa tcttacgaac acgatataac tctgttacaa aagtatcgtt gttaacaaca
    25921 gtttttccag cacgctttgc tccatgaagt ccgcagataa accaatcgtt attccaaatg
    25981 taatcaagaa cttgcaattg ctttcttgtg tataacttac tcaactccat ctgaaatagc
    26041 tcctttgacc atattcaaga aattggctat tttctcatct tgtccttcat ctccgccaat
    26101 ttgagacttc aacttctcaa tttcaaggtt gatctttttg agttctaggt ttgttgggta
    26161 acgtttcatc aattcgcttc ctgcttttat aacctctgct attgatggtt gcttctctat
    26221 cgttacaaat tcaccagtaa tctgattaag ttccgttact tcttcagtta attcctgtct
    26281 aagaatttgt gtaaacactc taagaacttc atcagctgtt gctatcgaac tcttttctaa
    26341 gactttcatg cgactctgta tagcttgctt tatttcaagt tttttcaagt tttgctcgcc
    26401 aatttgacca gcggtttttt tactataccc cgcttttatc gctgcatcag tcgcattccc
    26461 gcagatgatg tactcatcta taaatttttg ttgttttagt gttaatttac ctattttcca
    26521 tcacctcctt cgtgacataa taaaaagcca ccacaattgg tgactaattg gttaaccata
    26581 gataaatagc gaatgaatgc taagccgatt gcctacccca ttctgggaca tttctattta
    26641 tcgaacagga acagtcggaa tcgaaccgac acatataatc agaccgtcga caatccaatt
    26701 atcaaggcgc tacctctacc gttttccaat cacggttcat gttcataacg gaaagtgtag
    26761 gattcgaacc cacgcacgct tttacacgcc taacaaggta gcaacctgtc ctcttaaacc
    26821 acttgagtaa ctttccaaat gtaaacacgc tgagattaca cgtttagtca gttgtctttc
    26881 aaaaccttat gttttagttt tttatccact caaatccctt caagtaacaa ccatgtgcgg
    26941 ttagtatcgc taaccactcg ttacgccgca aactactaag ccatttttca attaacgaaa
    27001 accccgctaa aggtctaagc tgctttactc tttgactttg ctattatcct tgcgagactc
    27061 tagcaggtag cctagctacc gaagcacact ttcgtttgcg acgggcaatg acttttgctt
    27121 tattccaata ttttcaacaa atagcattgt tttcgtgtat cgcagacgtg cattgccttg
    27181 cgtttcgtcg ccttttgagc aacaaaatgc gcaacgccta cttgttatct cggtgctggt
    27241 acaccttgac ttgttgtgtt tgactgggat atcctcccag cgttgcacaa catgctgacc
    27301 gctcttggta ccacccttga acttcgtcag tttatacctc ctacacactc gtcgcacgta
    27361 ctgccgacac agcacctcac cgattggctc tggtattgcg ctttgacttc gtgtgctgta
    27421 accattactg attacataaa gattggattg cttagattga ccattactgg cgctcttgtt
    27481 gatacaccta caaaagcttt cccatatcgc tatggattat ctgtgctaag ccactattga
    27541 gacggcagga ttcgaagctg cacgtcccac atacataaaa tagcaagttc gatagtagtt
    27601 aaagttgata actaaataaa aagtctaatg gcaaaatgtt tatctcttct cgctatttcg
    27661 ataatactat aatagcacaa cgattacttt aatgagctgt aattcgctac gaattaatac
    27721 tataatctgt ttttatttac taattacttc atttaattta ataatcgctt tgcgtttaat
    27781 gctataaaat gtagaaccgc tgatattcag tccgtcccaa gcttcttcaa acgtgtcata
    27841 agtaagatat gcagcgatta acacattaat ctgcccgata tcatcaagct tatatatgtt
    27901 gtctaacaac tttgtctttt ctttttgcaa ttcagatatc ttatcaagat accattcgtt
    27961 ttgagatata atcgcaatat tcttatcttc ttgcgattgt ctaatacctc ccgaaacctt
    28021 catatcggaa aactgcgaac tggttatcaa gatattactg caattcattt ctttttcaag
    28081 ttctcttatt gatagcttga gacctttcaa cctttttagc attagttcag cttttgtctg
    28141 atttcgactc atttaagcag ctccttatga tataatgtaa ttaggatttt atattggagc
    28201 tggcttgcgt gagcctgctt tttttattac ctctctttcc cttttctgcc gactattttt
    28261 ttgtgttgtt aattgtcgag tattaaattt ttagttttgt gtcagcacta tattttcagc
    28321 gttgcgcttg tataatcatc tgtgagcgat aacagacttt agatttttat gaaaaaaatg
    28381 tcggaggata tttccctttc taaaaaattt cgctctataa ctagcagact aattattcca
    28441 aatctgctag ctgactacta cagaaagttt ctaagctgag tttaacgaga atcctagctc
    28501 gtacacccac agagccattg caggctctta ggcgcttgcg tgggacttta atttgcttct
    28561 gtgtttaata gtttaaaatg ccaagtttca tattcaccat gataaacgaa gcctacatag
    28621 ccttcatcaa cgattttatc gcatacaaca tatgctaaat cagtattttt taaataatct
    28681 ttttcaccat atttaacaat agcaatatca tgtttttcac cacttctaaa ataatagcca
    28741 gaggacaaat tatatttgtc attgttaaaa tcatttgcat attttttgga tataaaaatt
    28801 gttttttctt tcattccacc acctcttctc taaactgcca cgcccagtcg aaatctttgc
    28861 ggatttcgga ttctgttagt tgtaaatcat tatctgtctt tagtaagtct aagttatctc
    28921 tatgcataac tttgatactt acatttccgc taagttgtct catcagcaca aaacttaact
    28981 gtctttcatt cggattaggt atctcaactg tataaagctt ctctttttca attgtgatat
    29041 ttggataagc tagccaagct tcataaaact cacgttcatt gtgagttagc cactctctaa
    29101 cttcatcaga ttgtcgactc atgtgttgat gtaaataatc tacatcatca tcaaagcttt
    29161 taatcacatc aaatatcatt tgtggcactt ctggttttgg ttggtcaagt gtgtcaagca
    29221 aggcttttac cttatgggct ggaatcattg gaatcttcca aagctcatca ctatctactg
    29281 acaatttgtc cactattttt ttcgcttctt caatgttcat ttcgtcacct cattctattc
    29341 tggatatgtt tctgcattga taaaaaactt atcaggatgc ttgttccaag tcattacgtt
    29401 tttagcttta gttaaaactc cttcgtcttt ttcgtttatc cacacttcaa gttctgggtc
    29461 aactttagct agcactttat gtaaatcttt aactttcatt tgtcacctct tttaatctac
    29521 gtccacagaa aggacaataa ttgaatttaa caccagtaag attaaatatt cttccagttg
    29581 gtgagtcttc gcagttagcc aagaaatacc agttttgtcc tcttttttcc cagtaacata
    29641 aataattttc catcttatcc tccattcgtc caataaatac acaaatctct acttaacaca
    29701 actggtttac caaatatttt atacgacgat tcaagatttg ttatttcaac atcaaaaccg
    29761 tcccctagcc gttgttttag taaatctatt gttttttgac tgtcaagtcg tctagctaag
    29821 tattcatcgt ccttggggat agcgatttta tagccagaga aacctttcat cgcagattgt
    29881 ttaagcttct cctcgatttt taaatcatca aaaaatctat caaaccattt tttgtgtgat
    29941 tctgaaccgc attcttttac ttcatcaatt aatgacatcc tcatcctcca ttcccagtca
    30001 attctgcaaa ctttacaaat gtcatccagt gagttgttcc tcgtcgctgc ccaaaaagtg
    30061 gtgtaaacgg aattgctgat aaaacttctt ttacatttat ctgacaatca gaccatttaa
    30121 aaactaaagt tccacctact tttagaactc tcatgcattc ttcaaatcct tttgaaatat
    30181 cttctttcca attgtcttta tcaagttgcc cgtattgcgc tttcataatt gacttttggc
    30241 caacatattt taagtgtggt gggtcgaaga caactaagtt aaatatatta ttttcaaatg
    30301 gcatatttct gaaatctccc aaaacatcag gtttaacatt tacatgtttt ccgtgaatat
    30361 caaatgtttc ttgtctaata tccataaaag tggtgtgttc ttcattttta tcaaaccaaa
    30421 ataggcggct tccgcaacaa gcatctaaaa tttttatgtc tgacatccct atcccccatt
    30481 tccagtcaaa atcagcaatc cgctttgtct gtctctgatt ttgctcgcta gcacgtttaa
    30541 gttgcttttg tgtcctgcgt aatgcagtca agtatttctt gttcttcaat catccttcta
    30601 acctttctag taattctgga ttttcgtgta tatttccgat attttcgatt tcccgaattt
    30661 cgtctggaca cccgtctttg tagttgtaaa atggatcgtg tgtatctgct ttatcaacta
    30721 tgttccaacc aaaatctaca aattttactt gtcctacgta ctcagacaaa gagtcgttaa
    30781 aaagtctgca acacttaacg atatccccat caaacacctc aatgccgttt ttatctttta
    30841 ggcctgttga ttgcattaac acagcttcct cgaaagggat atatctacgg acaactttcc
    30901 gataaaaggt gtatattttg ttatcgacgt aatctattgc ttcaaccacg cccatagctt
    30961 gcgttacttt atcccacact ctaaattttg gtatcatcct taacctccaa ccaaaccgct
    31021 aacatcacgc aataattagc catgtcgttt aacgtgtctg acaggctttc tgagacgttt
    31081 ttgtcactgt ttataagatt atataacctg ttgtatttat cgcttatacg gacgacacca
    31141 gcgataaatc cgaagtcatt caaagacttt tcgaacgagt tcccataatc tgcattttta
    31201 gccaaaaaca tttgataatt ttcgttgtat gcagcttgca tactctctgc gtttatttta
    31261 tctgtcatgt gttatacctc taaaaactca tagctgcata tatcagacgc ttagtctgct
    31321 tgcaatgctc taacttagta tctttgtgcg ctctttttag ttttacaaaa agttccgtct
    31381 cgtggtcatt tgggttgtga tactcacgat atgatttgat atacatctgt gcataggtat
    31441 cttcgtcaaa ataatcttta aacgcttcga taacgtacgg tcttggcagg gtttttcgac
    31501 gtctgttatt tgtaacgcta catcttattt gctcggcttt tttgcaatct acatctagct
    31561 ttttaatttg ccttacaatc ccattgtcaa aaattttgta aaatttattt attaattcat
    31621 ctgtcaatct cttcaatcct cacttttatt cttggattct gactgtattt tttctttgct
    31681 cttaaatcgc atacgatatt gtcatctgac caaacgatac ctgatttctg tattctgtcg
    31741 taacctgcat cggaaatact atcaaaaaca gctttaatca gattatcaat atcaggcttc
    31801 ttagcgtgcc atataagctc acgcacgaag ttctgatata tttgtattgt tttaccttta
    31861 gaacgttgtg taggctcttt tgatagcgtt ttgtgggctt tcatgtaaaa ggttacctct
    31921 acctttatgc aatcatcaaa aaacggtcca tcataatttt tttctatcca gccagaaacc
    31981 tcttttcgcc acctcttcat ctttggatct tcgtacgtac caaatttgct gaacttaggt
    32041 ctagtttgag gttttggttc aattggtatt tcaaattctg ttttaaaagt catattcctc
    32101 ttcaatttct actaacaatg caattcgttt tgagctagct aacgcttgat atgatttagt
    32161 catgtattgc tctattgttt gctttttaat tccaagccgt gccgataact cttcttttgt
    32221 gccaacatcg acaaacttgt cgtcatcata tattgcatat atcctttgtt tcttaaacat
    32281 ttttcaaaaa tccacactcg ccctattaaa tgtgtgagct gtggcaagga cgagtgtagc
    32341 aattctccat attatcgatt ttatcgataa gcagactatt tcctttctcg ctcggaaaat
    32401 atagttactg caaaggccga gcttcacttt gcaatgttag ttaaaaaatc attactcttt
    32461 gtgttaattg attagcccta caatattcgc aatggccgca aggttttggg ggttctatcc
    32521 cttttttgac tgcatctaaa tgtttgatgt tttgtgctag gttatctaac tcattttgca
    32581 tagcatctaa attttcgatt gctattgctc tagtatctgg aggtgtttct ttagtcactg
    32641 cgtaaatgat tggcttaaat ggcttcttgt atttagcttc tagcatgatt ttataagcag
    32701 ccatctgtaa gatgtaaccg taagcctcaa accagtaaac tcgctcttga ccattccaga
    32761 ccgtgtcgtc aatagggcct tttgtagttt tgatatctac aaaatagcca cattcaacat
    32821 taagacagtc tatcttgcct ttgaattcaa cctcaccaag aaatcctgtg atggctacct
    32881 cttttttacc ttgataatat ttcatgaatt gataatcatt tttaagtgct tcaatcatct
    32941 gttctgcgac taaatagtct tttttgagct gacctttggt tgccccgcga ctcgaaatca
    33001 tttcagagcc gttttgggct ttgaattctt catgagcttt ttcactctca aaataagagt
    33061 ggacataatt cccgacgagc aatgcagtgt tatctctagt atctgtacaa tcccctcgta
    33121 attcagcaag cgccctcgtt tcgcattctc taaaacgctt gtactgacta atagaccagt
    33181 acttaattga tgattcattg ctataatagt cctttccaag caaatctaac ttcgtcatgg
    33241 cattaagtcc ccaaggttat caaataggtt gccttcgcta gctttaattt caccagtttc
    33301 ttggtcaaaa tccggaattt catctgccgg ataagaggta tcttctaaaa ccgtcttatt
    33361 ttcgtctgtg agcggttttt cttcttcatc atgtaaatca tctgttatat ctttcaaatt
    33421 gctaggagcg tctatttttt cgttctgcgt gccgattaag tcatcaagac tatttgtctc
    33481 tggtggtgtg acatctttga cttggcgttc atcaacaaac tcatcagcag tagtcttatt
    33541 gatagaatcc gcaagaatgt cactgtcatc actaatgttg ataaagaact tagcagctct
    33601 attgatgact gttttcttag ccatctcttg tgggaattct ttttgtgttc cgccgcctgt
    33661 ttttgttttt gaccaggact tatcgatttc tttttttgtc atcaatgtta gctttttatt
    33721 gccatcaaca tcttcgataa cgcaataagc tccttcaatt ggattatctt ggtttttcca
    33781 atgtgttgag tgttttttaa atttcttttc gccatctacg atttcaagtt caaattcatc
    33841 accttcgtag ataacttctg ctttaattga tttgattttt tgttgttgga gaagaacagc
    33901 cattgtccca aaatatgaac gttgaagttg taattcattt ccatagacaa tgaaatagca
    33961 ttggcttttt gctggtgata acccttgcgt taccatttct aatagtgcat tagcaatcga
    34021 atcctttgta caaacttcaa gggcggggcg cttgtttctg tctttggtct ttaaaatttt
    34081 aaagaatgct gattttaaag catttgctgg agcatagttt ggagcaatta ccaaaccttc
    34141 atcttgtaaa gaactaattt ttgaattaac gatatcagtg atatcttttt gaattgttac
    34201 aatttcactt gccatctcta ttcacctcca aaaacctgct caaacattcc atttactatg
    34261 cttttaattt tttgctcttt tgttaactct ggaacatcct cgccatcaat aaattttagg
    34321 tcatatgatg cttcgataac tacaacatca cacccaagcg tttctgccaa attatcaatt
    34381 ttttcttttt gcatgttgta agcttcttct ggtaaaaatg atgccagatg aatgctatct
    34441 gtaagttcta cattataagc aagtacatct tttttgtttt tgaaactctt taaaaaactt
    34501 ccgtcttcag tgtttcttag cactacaatt ttttctttgg tgttcatttc atttcctctt
    34561 tctatgtttc aattgccaat tttcggcttt taagctcttc aactgtttct ttaactctat
    34621 attttcttca gcttctttaa gataatcaga catcaagtcg ctgtatctac tttgccaata
    34681 acgactagac tcatatattt cttcattcac gtcagtcctc taaaattcgt tgtttgatct
    34741 tccacttact gtctgaatta gtatcaacaa acatttctgg gtctacatca aattctgtgc
    34801 tgatatattc catcaagtcc tcgtctgtat agtctttaaa ttcgttgtaa gtctgcctta
    34861 gcgtaggctc ttcgctgtct cgtaaatagt caattgtaaa tataaaagca tccctaaaat
    34921 taccgtcaaa cgttacacgt tcgccattaa tcctaatttc taccatgata gctacctgcg
    34981 aatttctcta gtctatcttt aataaagtca aacattgctc gcaactcatt gttttctttt
    35041 cttagatttt tgttattaat cataatatct gccatagagc catctttttc tcgatattca
    35101 tcttttaaat gtttaacatc ttcagacaaa tcaatgtttt tagactttaa gatttcattt
    35161 tcaattttta agtctttaat cctattttct aattcagcta ctaatttcaa atctggtcta
    35221 ttttccaaag tcaatcctcc ctctgcgcag tcttaaccgc ctgtattctt caattttttt
    35281 atttcgacta ggttcgtcta gagccatgat ttttgctgca tgttcttctg acaagctgaa
    35341 aaatgttgtt aatgttagtt ccataatttc attctttcat cttccattcc ttcaaattcc
    35401 atgatatggc ttttatcaca accttttcgt atacgtgatg caattctctc tccatacgcc
    35461 tgtctaattt cagctggtgt aagatttgtc gtgatgattg tatttgtacg cttgttaagt
    35521 aagctatata taatacttgt cgaccaatcg ctaacctttt cagcacctaa atcgtccaaa
    35581 actagataat caacctcttt taatttgtcc aaccaaaacg cctctttact aaagtctcgc
    35641 tttatttctg ataacaaatc agtaacgttt acaagtagtc ctagcttctt cgtcttatct
    35701 gacagtcctc tgataatgct gtaagctaga tgacttttgc ctcgtccagc tttaccagtc
    35761 atgataatgt taccctgacc tcctttaaac caatcgttag ccattgtttt agcccaaaca
    35821 agcacttctt tatgtttgat tgtgtcagtt ctaaaattat caaacgatgc gttttccagt
    35881 tcgctatcca tgattgataa cctttttaga tagtacagcc gtttgttttc gagttctctc
    35941 tcatattgtt tttgaacgtg taaatcgttt tgattttcca aatcttcttt gtggcattta
    36001 gggcaaactg tcaaaccagt tttaaggatt gtgatatagc tacagtcatg cttgtcacaa
    36061 attgtctctt cttttttggt atttttttga taggacaaag cgattttatc aagcgcattc
    36121 tcatcaccaa gtatcatact cacttacctc ttcttgctta gatttcctag atttctcttt
    36181 agcttctatt tgctcaattg tcgtgatgtt gtcatctctc cagttacgta aaatacctct
    36241 aacatagtta agattagttt ttccttgaag cttagttctt ttgatagctt ccttaattaa
    36301 gtcatgatta ttttctttaa tcatcgtacg aatagtttca atttccatag gagacaacaa
    36361 ccgaccaaat tcttgttccg ctatactata tatatagtta gtagttgtct gattagaagg
    36421 cactaagttt tggttattta gtattgatat attagtattg atattattag tattgattc
//
LOCUS       DATROY010000008        29956 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.008, whole genome shotgun sequence.
ACCESSION   DATROY010000008 DATROY010000000
VERSION     DATROY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29956
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1116)
                     /locus_tag="VBD11_000361"
     CDS             complement(<1..1116)
                     /locus_tag="VBD11_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6953338.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDT"
     gene            complement(1203..1706)
                     /locus_tag="VBD11_000362"
     CDS             complement(1203..1706)
                     /locus_tag="VBD11_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6953339.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(1792..3123)
                     /locus_tag="VBD11_000363"
     CDS             complement(1792..3123)
                     /locus_tag="VBD11_000363"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6953340.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDSTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(3133..3750)
                     /locus_tag="VBD11_000364"
     CDS             complement(3133..3750)
                     /locus_tag="VBD11_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953341.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(3915..7013)
                     /locus_tag="VBD11_000365"
     CDS             complement(3915..7013)
                     /locus_tag="VBD11_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6953342.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(7168..8478)
                     /gene="der"
                     /locus_tag="VBD11_000366"
     CDS             complement(7168..8478)
                     /gene="der"
                     /locus_tag="VBD11_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6953343.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(8526..9428)
                     /gene="dnaI"
                     /locus_tag="VBD11_000367"
     CDS             complement(8526..9428)
                     /gene="dnaI"
                     /locus_tag="VBD11_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6953344.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(9425..10600)
                     /locus_tag="VBD11_000368"
     CDS             complement(9425..10600)
                     /locus_tag="VBD11_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6953345.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(10600..11079)
                     /gene="nrdR"
                     /locus_tag="VBD11_000369"
     CDS             complement(10600..11079)
                     /gene="nrdR"
                     /locus_tag="VBD11_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6953346.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(11228..12733)
                     /locus_tag="VBD11_000370"
     CDS             complement(11228..12733)
                     /locus_tag="VBD11_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6953347.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIHKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(12723..13412)
                     /locus_tag="VBD11_000371"
     CDS             complement(12723..13412)
                     /locus_tag="VBD11_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953348.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(13647..14180)
                     /locus_tag="VBD11_000372"
     CDS             complement(13647..14180)
                     /locus_tag="VBD11_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6953349.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(14285..15175)
                     /gene="htpX"
                     /locus_tag="VBD11_000373"
     CDS             complement(14285..15175)
                     /gene="htpX"
                     /locus_tag="VBD11_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6953350.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(15196..15750)
                     /locus_tag="VBD11_000374"
     CDS             complement(15196..15750)
                     /locus_tag="VBD11_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6953351.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            15878..16591
                     /gene="rsmG"
                     /locus_tag="VBD11_000375"
     CDS             15878..16591
                     /gene="rsmG"
                     /locus_tag="VBD11_000375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO6953352.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            16606..17985
                     /locus_tag="VBD11_000376"
     CDS             16606..17985
                     /locus_tag="VBD11_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6953353.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLSHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            17998..18669
                     /locus_tag="VBD11_000377"
     CDS             17998..18669
                     /locus_tag="VBD11_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6953354.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(18704..19534)
                     /locus_tag="VBD11_000378"
     CDS             complement(18704..19534)
                     /locus_tag="VBD11_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6953355.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(19527..21203)
                     /locus_tag="VBD11_000379"
     CDS             complement(19527..21203)
                     /locus_tag="VBD11_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953356.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(21220..21768)
                     /locus_tag="VBD11_000380"
     CDS             complement(21220..21768)
                     /locus_tag="VBD11_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6953357.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(21937..23145)
                     /gene="sstT"
                     /locus_tag="VBD11_000381"
     CDS             complement(21937..23145)
                     /gene="sstT"
                     /locus_tag="VBD11_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6953358.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            23321..24688
                     /gene="brnQ"
                     /locus_tag="VBD11_000382"
     CDS             23321..24688
                     /gene="brnQ"
                     /locus_tag="VBD11_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6953359.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            24802..25857
                     /locus_tag="VBD11_000383"
     CDS             24802..25857
                     /locus_tag="VBD11_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6953360.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(25906..26598)
                     /locus_tag="VBD11_000384"
     CDS             complement(25906..26598)
                     /locus_tag="VBD11_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953361.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(26595..27665)
                     /locus_tag="VBD11_000385"
     CDS             complement(26595..27665)
                     /locus_tag="VBD11_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6953362.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(27658..29034)
                     /locus_tag="VBD11_000386"
     CDS             complement(27658..29034)
                     /locus_tag="VBD11_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6953363.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(29168..>29956)
                     /locus_tag="VBD11_000387"
     CDS             complement(29168..>29956)
                     /locus_tag="VBD11_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6953364.1"
                     /translation="TTLLGVTFSNQEVSASSTSSKVVKVGVMTFSDTEKARWDKIEKL
                     VGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENKKNLIPLEKTYLA
                     PIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLNVSGKKVATVANI
                     TSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIFVEKSDKNSKQWI
                     NIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
BASE COUNT         9436 a         5967 c         4625 g         9928 t
ORIGIN      
        1 tgtatcaatt ttattagact caatagcttt agcaatctgt ttaatagtgt aaccctcggt
       61 aattaatatc ttacctaaag ctggttttgt cggttctgca gttccgcctt tttccaattc
      121 agcagcaatc tgatctaaag tcatactttt tttaagattg tagtatccac tttgaaaatt
      181 actatagttt ttgaacttgg aatagtaatt aaagactgtg gcgctcttaa tgactcctgc
      241 tttttctaaa atttgaccaa ttaatttatt cccagatcct atcggaattt caacttgtac
      301 aaatttattt gaattggaat caacaggttt aatagcacta tctacaaaac gataacctac
      361 aaatgctgtc aagagcaata caagtatgat aattgatatc agaatacgtg caattcgttt
      421 tgccacatta tctgctttct gacgtctttc acgcttgtta tgtttgttct tggctgacaa
      481 agctataccc tcttcttctt ttgacaaatc ctctactcgg ataggttcct ttgctacggg
      541 ctctaactct gatgtctcac caagttttac attttcacct tctgtaggaa tatctgttgc
      601 tctcacaaga aagatttctt ccgtttcttg attaggcaca gcagttgcaa catcagtctt
      661 atctgctttt aaatctatgt caggtacatt ttcagttgtc ctaaattcac tagatttgga
      721 gccgctatca agctctcttg aaggttccat cccttgattg ataacttgtt cttcttggga
      781 agctaatgct tgttccgcct gacgtctttc ttcttctcga gcagcttctg ccagaagttt
      841 cgcctcaatc cgagccttct cttgctcttc taagcgttgc ttagtctcta gcgcctttgc
      901 tctagcttct tgttcgtctt tcaaacgttg cgcttcataa tctgccatca attgtgctgt
      961 tctgcgagca gcttctttgg cttcttgctc tttttgatag agttcttctt cacgcaactt
     1021 tctcaaacgg ttagcttctt ctaactctgc cagaatttgt tctttaaaac ttttctgatc
     1081 atggttagaa tgctgatcgt cattaaattc ggtcaaagag atttcctcct aataataatc
     1141 acaggcaatt atatcttaaa tagtctataa aatcaacaaa actttgacaa tgttatctac
     1201 ttttaacaaa ttgatcgtgt tggattattc caaatcatat tataccaaag cgaaccacca
     1261 tgttgggagt cagattctcc ctcatcttta aatccattca tttcatagaa ggaaattaaa
     1321 tcatcatggc aagttaaact tataccatct cgttcttgag aaacaaccag gtctttcatc
     1381 gctgctaata atgctgtacc aatcccctga cctttaaaat caggatgtat tgatagacta
     1441 gtaattccta tgaaaccacc tacccttaca gggaattcag aaactttatg gaataaatcg
     1501 tccgttaagt accgaccttt aataaccggt ccttcaatgt aacctgctaa acgaccatta
     1561 atctctgcaa ctaaaaaagt atcagtcatt atcgttaaac gttcttgcat tgctttttta
     1621 ctcgcagcct ctgcttctga aaaatttatt tgttcaatac taatgacttg ctgtaaatcc
     1681 tctaggcaac catttctaat aatcatactc atctccttaa tttctttatg aaattctctt
     1741 ggtaaagtca gatagaggac tgagaaactt ctctcagccc ttaccatcaa cttattgtgt
     1801 atttttagtt aggttagcta ataattcttc aaaagagcgc tcatacaatt gaatgtctcc
     1861 agcacccata aagacataga cagcattatc atgattgagt aaaggcgaga cattttcgac
     1921 tgtcactaaa tctgagtgtt tgacaatctt agcagctaaa tcttctacct tcacctcacc
     1981 attatctact tctctagcag aaccatatat ttgagcgaga taaacgctat ccgcttgact
     2041 caaggcatgg gcaaattcgt ctaaaagagc tatcgtacga gtgaacgtat gcggttggaa
     2101 aatagctaca atttcttttg acgggtattt ttgtcgagca gcatctaatg tcgcaataat
     2161 ctcagtagga tggtgagcaa agtcatcaat aatgacagta tcgtcaataa tcttctcagt
     2221 aaaacgacgc tttacccctg aaaatgtctt caaatgctca gctactaatg ccatatcaat
     2281 tcccattatg taaaggttag caataacagc agttgcattt aagatattat gtttaccgta
     2341 tgctggtaca tgaaactgac caatttcttc ttggttatag aaaaccttaa agtcagaacc
     2401 attaacagtt cgagtgatgt cttttgctat aaaatcattt gaatcttcaa aaccataata
     2461 atatattggt gcctcagaag tgatttcatg aagttttgga tcttctccat aaatgaataa
     2521 acctttttga acttgcttag catagtcatt aaaggcattg aatacgtcct ctaggcctgt
     2581 aaaataatca ggatggtcaa aatcaatatt ggtaataatt gagtattctg gatggtacgg
     2641 cataaaatga cgttcgtatt catcagcttc aaacacaaag taattagcat tagcagaacc
     2701 acgtcctgta ctatctccaa ttaggaaaga agtgtctgta atatttttta aaacatgagc
     2761 taataaacct gtcgttgagg tttttccatg tgccccagct acacctagac tagtgaactg
     2821 acgcataaaa tctccgagaa attcatgata tcgtttaaaa tgatagccct tttcaataac
     2881 ataagccaac tcttcattgt tatctggacg aaaagcattt cctgcaataa tctctaaatc
     2941 ctcactgata ttattcggtg agaaaggtaa tatagttaca cctgcttgct ctaaaccacg
     3001 ttgggtaaaa taatatttgt caacgtcact tccttggacg ttatgtccca tttgatgaag
     3061 catcagtgct agggcactca ttccggatcc tttaatacca ataaaatgat aagtttttga
     3121 catggtctct ccttaattaa tgtctttaaa acgtgtcaga tttaattctt gagccttggc
     3181 tctttcttta tgaaactgat tgtcttgttt attgtaaact tgactgcgtt tcaaaaaatc
     3241 aaaattattt ttctttgttt ttgggttcgg tgtattatca ggttcttgaa atatgattgg
     3301 aatttctgct aatatatagc tttcctgctg taaacgatca gaaagatgac ttagttcagt
     3361 cacctttcgc tgagtttgtt ttttcggtac tgttgccttc aattgttgct gaaaagcttc
     3421 ctttgatggc gccttaaatt cctgtttcgc atagtaagct tgtcgtttct cccgaacaga
     3481 cctctttgcc tcttgtcgag atttgctcaa ctctgtcgct ttttttacaa aactctgcgg
     3541 tggtacagga tcattttcaa ttaccttagg atgtgatatt tcttcatgtt cataattgct
     3601 tttgaccatg ctttgatatg ttttttcttg ataaaagcct cgaatgttgt taattaaatc
     3661 ttcattatca taaagattca tctttacaag cggagacatt actggttcat catctgctat
     3721 caacggaaat cgtctagagc ctctattcac attatcactt cctttctaca acagacctat
     3781 tataaactaa tttttctgtt ttttcaaagt tttcaactaa gtaaagctta tcagattggt
     3841 atttttcatt catttatata aaaaagaaag cgagagacca ctttcttttt tatattatag
     3901 tcacatcatc atatttattt agaaactcca agtatctctc gaatatcatc tacgctcatg
     3961 ctagcatggg tttcatttcc atccagaact gttgtaacca aatgtttttt agtttcttgc
     4021 atttctagaa ttttttcttc gattgtgcct ctagtaatca agcgataaac ctcgacattc
     4081 tctttttgcc ccaacctatg tgcgcgacta atcgcctgca tttcaacagc tggattccac
     4141 cacaagtcaa tcagaacaac ggtatcagct cctgtaaggt tgagaccaac gccaccagct
     4201 tttaaagaaa tcaagaaagc atcttttgat cctgcattaa atgctctggt catttcatga
     4261 cgttcatttg ctggtgtaga accagtaatt ttataagttg taagccccat agctaccatc
     4321 tcacgttctg cgatatctaa catacccctg aactgagaaa aaatcagagc acgatgtcca
     4381 ttttccttaa tttgtgttaa aagttgacgt aggctttcta acttaccgct ttcaccatca
     4441 taatccataa aaagacgtgg agtgtcacaa atttgacgta atcgtgtgat gccagataaa
     4501 atttcaattt tacgacgatt taagtcagcg tcactagaat gttggatact ttcttgaatt
     4561 tggcgtaatt gcgccaaata gataactttt tgactgtctg tcatttcatt tggatagttc
     4621 atttcaatca aatccggtaa ttctgggaga acttcttctt ttcgacgtct catcacaaat
     4681 ggtttgatat aacgagcaac ttgtttaggg ttcaacttca aaaactcttt cttaccaggt
     4741 aataaaccag gtaaaatgat ctggaaaatg gaccaaattt ctaacagttt attctcaata
     4801 ggtgttcccg ataatgcaaa acagtttttg acctcaaaag atctcaagct atgggctatt
     4861 ttagtttgag catttttcat aacctgtgct tcatccaaaa ttaaataatc ataatttcct
     4921 tcagaataag tctcaaaatc ctgcctaaat gatgaataac ttgtaatgat tacttgatga
     4981 cgttcagcaa taatttcatc tctacgaatc ttagaaccat aggcaactgc tacatctacg
     5041 tctggcgcaa atttgtggaa ttcatcttgc caattataaa ttaaacttga tggtgataaa
     5101 ataagcacac gagaatctct agttagtttt gtacttaaaa atgaaatcgt ttgaagtgtt
     5161 tttccaagcc ccatatcatc agctaaaatc cctgcaaagc cataatgatc taacatactt
     5221 aaccaacgga cacctgtaag ctgataatcg cgcaaggaag ctgaaacaga tagaccaggt
     5281 attttaaact tttctgggtg tctcaaatct tcaatcaatt ggtaaaaatg ttgtgaaaag
     5341 ctaacattat cttgatttgc aaacagttct gataattgaa atgctgcaat attatctaat
     5401 tcaatatgcc cattctttgc tcgtctagct ctcaaccctt gcaaagttgc acttactttc
     5461 ttagtttctt catcaaaaat aactaattgc cctgatttat tcacaaaata aggattattg
     5521 tcaaacaaag ctaccagagc ttgatcaaca tcgttttcca gtaaatcaga gaaatcaaaa
     5581 gaaatatcta gcaggcttcc cttagcttca atatcaattt gcggtcgttc tatgagacga
     5641 taactttcca attttgcgga taagctcact gttcccattt ttttaaattg tggaagcata
     5701 taggtataaa aatcgtataa ttcttgtgca ctgtatgctg gatggctggt tgaaaagtgg
     5761 ggtgcaaagc catatttttc taagagttta aatacttttt cttcatgttt gaagtgacta
     5821 gcaaatgtta actgttctag ttcttgacga ttatgaacag ttaaatcatt accataatca
     5881 aataccattt gcaataaaat ttcattttga ctattaatat cgaactcaaa atttacctta
     5941 aaatcatgaa tagagaagct ccgtggagca tccactagtc caatctcctt aaaatccagt
     6001 aaatgagctg ctaacttatc ttgatcatct aaatcaaaat ggatatgttt tgctaaatct
     6061 ccctcaatgg gaagactacg aatagccgtt accatctttt cttgcttcaa attaagatgg
     6121 tagaaagtat cgcgataaaa caaataagaa ttcgcaaata gtggttttaa gtctttctct
     6181 ttgatttcta attcaattga ttgcctatgg acaataacct taaattgata aagatgatcc
     6241 tcaccttcta attccttaaa gtagaggtga ttaaattgat gaaaatcact ttcaaatgaa
     6301 aaattttcta aagcattcat caaagtaaca ccctcttcaa aaaaaccacg agtgagacta
     6361 aggtgacgac cttgatttgg taaaataaac tctagatcaa ttttgcttga atgacttgga
     6421 atgagcctcc ataaaaattc tatcaattct tgactggtct catcaaactg tatcaaagac
     6481 aaagtttcaa agtattgttt tccaatctga taataagctt cttttcgaac agtatttaaa
     6541 aaggctttga tgtcacgaat aacataactt ctatcatcag gcagtcgtct aatttttaaa
     6601 gtccaccaga tatcattagc ataaggattt tgttctcctt gagccgataa ttgatatttt
     6661 atagtatcat cttcattaat aattagacta tctaaaaaaa cactaccaaa agaggttttc
     6721 ttcttagttt cttgttgact ttcttgttga acttgaactt tagataagat tgctttacct
     6781 tctggatcgt ttttcaagaa atgctccaga gcagctaaat gttgacaata gccttttctt
     6841 gcaaaaaaat cacaagaaca ttttgtttct tctgttacta atgaatattc aatctgacaa
     6901 tccccaactt tagcttttaa tagattacct tcttgagaaa tcaaggacac caaaccttgt
     6961 tcgtagagtt ctataccttg atttcgaata cgtcctggaa tcattctact cattatgcta
     7021 ccaccttcat cttatccctt aattataaca aaattcaagt ccaatttctt gtaaaaatac
     7081 taaattgatt gttttttgat acagtaacaa ataaaaaagg ctaaaacaac tgtcctaacc
     7141 ttctatttca atttcaacaa taagatgtta tttacgttta cgtgctatta gattaattgg
     7201 tgttccttca aaaacaaatg cttcacggat ttgattttct aagaaacgaa gatatgaaaa
     7261 gtgcatcaat tcttcttcgt taacaaaaac aacgaaagtt ggtggtttaa cagcaacttg
     7321 agttgcgtag aaaatcttaa gtcgttttcc tttatcagtt ggtgttggat taatagcaat
     7381 agcatccata atcacatcat ttaatacagc agacggaata cgtttgtttt gactttcgct
     7441 aatacgttta atcatatctg gtaacttgtg caaacgttgc tttgtctctg cagaaacaaa
     7501 gataattggc gcataagaca agaattggaa gttatcacgt atatcagctt cccactgact
     7561 gacagtatga ttgtcttttt cgatagtatc ccatttatta actacaataa taataccttt
     7621 accagtttca tgtgcaaacc ctgcaatacg cttgtcatac tcacgaatac cttcctctgc
     7681 attaatcacc ataagaacaa catctgagcg gtcgatggcg cgcatagaac gcataacaga
     7741 atatttctct gtgttttcat ataccttacc agatttacgc ataccagcag tatcaatcat
     7801 agtatactct tgcccctggc tatcaacgaa attagtatca atagcatcgc gcgtagtacc
     7861 agcaactggg ctagcaataa cacggtcttc cccaaggatg gcattaatta aactagattt
     7921 cccaacattt ggacgtccaa tcaaactaaa acgaataata tctggatttt cattttcctc
     7981 ttctaccgga agattttcca caatagcatc caatatatca ccagtaccaa taccatgaac
     8041 tgatgacaag ggataaggat cccctaaacc aagagagtag aaatcgtaaa tatcattgcg
     8101 catctcagga ttatctacct tattaacagc taaaataacc ggtttattgg tcttataaag
     8161 aatacgtgag acatactcat ctgcatcagt cacaccttct ttacccgaca caacaaacac
     8221 aataacatca gcctctgtca tagcaatgtc tgcttggtgc ttaatttgct ccataaacgg
     8281 tgcatcaaca tcatcaatcc caccagtatc aattaatgaa aacttacgat ttagccactc
     8341 acccgttgta tagatacgat cacgagtgac tccttcaacg tcctcaacaa tggaaatacg
     8401 ttctcctgca attctgttaa ataatgttga ttttccaaca ttcggacgac caacaatagc
     8461 aactgtaggt aataccataa ttctcctctc actattggga atactcccaa ttccattcta
     8521 ttttattaac ggcgattcgg tccttctaag tggaattcta tcgctaaata ccgtacgcgc
     8581 tccataactc gttttgcttg ccaagtttcg tcgcttcctt ttatattagc ccattttctt
     8641 tccaaatcgt taaaactata atttgacgta aagaaagtgg gcaactcttc tagcatacga
     8701 tgttgtaata taacttgtaa aatctcgtca cgaacccagg acgttgcctg ttcagcccca
     8761 atatcatcta aaatcaaaat cggaacactt ttcacagcat caatttcatc ttttacagtt
     8821 ccgcttgaaa tagcattttt gacatcaata gcaaaactag ggaaatgaag aagagtcgtt
     8881 gacacaccct ttctctctga taattccctc gccattgctg ccattaaata ggatttccca
     8941 acccccatat ctccatataa ataaagcccc ttgtgattat atgaaggata agtctctaca
     9001 aaatcaagca gttgtgacat agctttcatt cgactctcgt tattaatatc aaagtcagtc
     9061 attctgatat tccgatagga cttgggtaga ttaactaaat taatacgatc actgatagct
     9121 tgtttttttt gtgcttcaat taactctcgt gtttctaaat aagaaacatc tgcataacct
     9181 tcattcataa ccaaaatagg ctcatagcct ttagcaatat attgactatc cttattttta
     9241 aatttctgac gctcaatcaa aaattgatta aatttagaca tgctgatatt tatttcacgc
     9301 tgagaaagtt ggtgggtttt aatgaactcc gctacctgag catctgctaa aatcatttga
     9361 ataagctcat catttgtatt acgaggcact ctcccttgat tttctaatgc ttgtcccact
     9421 gatttcatga ttcaccatcc tttcctaatc gttctaaacg cttgagggca tctgttttaa
     9481 actgttctaa ttcaatttct ttttctgggc taacctcatc tttataatca ggattactcc
     9541 attcaggaac attcgattgt ttcttcttcg tttctgtttt agtccttact ttttgatctg
     9601 aaaaatcacg aattttaagc acagcatctt cggccgtcat aacattctga aaggcaaaat
     9661 cattagcaac tttcatgata taggccttat ttaagttagc agattttgtc ttattcaagg
     9721 tatataaaac cataacatta atgacttcgt ctaggaaatt catttttgcc aaatcctcaa
     9781 gtaaagtctt ctcaccactt gttgtcactg ccttacggct tctcttgatt ttttcaagga
     9841 acactagagc actatcattt ttagattccc ttagtatgac cttttcatta tttgtaaaac
     9901 tctgattatc tttaatatgc tgcccctcat tttgttgaag tttcattcgc tgaggcgcaa
     9961 tcattccatt gatagctgtt tgttttgcta attgataggt atcataccaa tttaaatgat
    10021 aaagttctga tacactataa atacctaaga catcatcttt ttcattgtta aaacgtaagc
    10081 catctcgcat catcaaacgt ttgaaacttt ccaagtcaaa aacattttta ctccggttta
    10141 cctcttgttt tggttgtcct aaatccgaga aaacatctgt aaatttttta gagatatctc
    10201 tagcattttt aggtattttc atatctaatt ctgccactgc tacctctcct atacgttttt
    10261 ctaacaaacg acgatagatg ggattagata aaaataactc atttgacata gcttgattca
    10321 acttaacaag gtaagtacct gtggcttggt aaacagtcaa caaatccaaa gctgttaaca
    10381 ttaccaatgc atcttgaaat cttggcatcc cgtattgcag atgattgaga acctctgaaa
    10441 atttatgacg tcgtattccg tcatcaaaaa aatgaacaaa ataatcatag agtgccatgg
    10501 catcacttcc cataattggg aaatacaatt gtattaaact attactatca taaacaatct
    10561 tattttgctt aatatatgta aactcatcta ttggactcat cacacgctac cactcttttt
    10621 actccgtacg cgctttgtaa tttgttgaag tagttcttca atttcatcta catctttaaa
    10681 agatttataa acactagcaa agcgcacata agtaatctca tctaattcgg cgagttcatc
    10741 catcactaaa tttccgatag tgatactaga aacctcatct tcatactcac tcctaatttt
    10801 acgttcgata cgtaagatac aattttcaat atcttcgctc gaaacagggc gtttttgagc
    10861 actctgaata ataccattca aaattttatc tcgagaaaat tgctcgcgag taccatcttt
    10921 ctttatgact aaaagaggca attcttccaa acgttcaaat gttgtaaagc ggttgccaca
    10981 tttttcgcac tctctccggc gtctgatagt cgtaccttct tcagcttgtc ggctatcaac
    11041 cacactagat ttattatagc cacattttgg acaacgcatg tcaacacccc tattctatca
    11101 tatcatttaa tcatcttatt ttaacacaaa ttcagtaata acaaaagaag cgcctctctt
    11161 atcacctcat catatcaaaa cacaaaataa tccttatgcc ttttatcatt aagcataagg
    11221 atttttctta tatttcttta gtttcttcaa atttttttat aggaatgcga acaatgaatt
    11281 cagtcccttg atttagttca cttttaatat ccacctttaa atggtaagca tccataattt
    11341 gtttaaacac tgaaagcccg atacctaaac cagcttgtgt actttctctg ttacgtgatt
    11401 tatcagttcg ataaaaacga tcaaagataa actcaatatc ttcatctgag ataccttctc
    11461 ctttatcttt tacaactact gtcgcaaaat cattgtctac tgataaaaca acactgactt
    11521 ctttataact aggagagtac ttgatagcgt tatcaattaa gatcatgaga gcttgctcaa
    11581 aatgtatctt atgtatcttt ccccaaacga tatctgaaat attattttca aaaataaatt
    11641 gaaaatcttc tctcaaaata cggaaatttt caataacaac tgatatagag cttgataaat
    11701 ctgtcacttc atcttgatga agttccaagc ttccttggac gcgaatcata tttaacatgt
    11761 cattaatcat taaggacata cgatctgcct catggtatgc cgctgctaaa ctttcttcaa
    11821 gaatttctgg atcatctttc ccccaacgtt gtaataaacc aatatgaccc tttacaacag
    11881 cgacaggcgt tcttaattca tgactaacat cactaatgaa ttgtgattgt cgacgagtat
    11941 agtcttccaa ctgatccaac atcccatcaa aaatcctaga caattctcca atttcgtctt
    12001 ctgaacggac cttacttcta atttctaaat tactaggatt ttcagatatc tgatgcatta
    12061 agtcgtgtaa atttttaacc ggacgaacaa tacgcttagt agcactatta ataacgacca
    12121 aggctaaaac agtccctaaa acttccatca taatcaagaa aacaatgatg ttttgcctca
    12181 tactataata acgacctaaa ttatagaaaa tttgaacgta tccaagcaac tgtcctgttt
    12241 tttgagagta gattgctttt gtaccaacaa aaccttccat atctgcaact ttagagatga
    12301 taacttttcc catggattta ccaatagttg gcatacccgt tctcagtgta gaaaaaactg
    12361 gtttcctgtt gacatcgtat agggtcattt cttgattaac ataaagaata ctatcgattg
    12421 actcgcccgt tctaacaatt ccattttgac gcttatgagg atagatgtca tccgcaaaaa
    12481 cctggttgta caggatctct aaaatatcat cggatgtcaa actagagttc gcttgcgaca
    12541 aacgaactct agttttttct aatgacctac cgacattaat tttttccctg tgaaggacgt
    12601 atcttgttgc cgaagtataa actaaaacag aaaatactgt aaataggcta aaaaataaga
    12661 taaaaactaa ttgcgataat ttttttgaca gaggttgttt tacccctata aactgatctt
    12721 ttttattttt cacgaatcac atagcccatt ccgcgaacag tttgaatata tgattcacgg
    12781 cctgggatat caattttacc tcttaggtaa cggatataaa catcaacaac gtttgtctct
    12841 gctgccacat cgtatttcca aacatgttct agcaactctt ctcgtgtcat aacacgattc
    12901 atatttgtca tcaagacatt caacaaatca aattcacgtt ttgttagtga aatttcctca
    12961 tcaccacgca tcgctgaacg attatgagta tttaatgaca aatcacggaa actaccacta
    13021 tcaccttttt cttttgtctt cgtttcaatt tcttggcgtc ggaaaatcgc tctaacacgt
    13081 gctaataatt cttcgattgc aaacggctta acaatataat catctgctcc acgatcaaga
    13141 ccagctacaa tatccataac agaatcacgt gctgtcatca tcatgatata ggttgttttt
    13201 tcagcctgca ggcgacgtgt gatttcgaaa ccatccatct ctggaagcat taaatccagt
    13261 agaatcaaat caaaatcttt ttctaatgct gtgtccaatc cttcacgacc gtttgtttca
    13321 acaacaacat catatccttc atgtagtagt tctaacgaga cgaagcgagc taaatttttc
    13381 tcatcttcga ttattaagat ctttttaccc atctacacca accttcttat tctttactta
    13441 acactatcaa ttatttttta gaatatctag ataaaaaaca agatagccca cattttacta
    13501 acgtttcttt aaataaataa atcgccatcc caaacaattg ttcgatagcg agacttaatt
    13561 tttattattg agtagtgtcc tctgtcactt ttcaattcta ctaaaaaaaa attaaaatat
    13621 caactgatgt tattaataat ttttatctaa tcactatcaa acataccttc taaggctgcg
    13681 aaaggattag atttttcttt ttctttagct tgtttttctt catactgctt ttcagtcatt
    13741 aacgaccaat ttttacctga taaatccgct tctaccccaa cctcatcagc tgccagaaca
    13801 cgaagaggta tatttaacaa gatattatcg ataacgcttt cttctaaatt aatatcatct
    13861 tcctctaata ttaagaccaa ctcctgatca acgagctctt ttttagtgct aacattttca
    13921 gcttcaataa aaacttcatt aatagtttgc ttttcagata aaacaactgg tttcatagaa
    13981 cgactagacg gtaatgtaat atcgtaagcc atattatagt caagtagata gagaccatct
    14041 tcatacacca catgacctga aacttgaata tcagaaatat ccattatctc tgaatgacgc
    14101 tccataagcg actctttgat atctattttt ttatcaaaat aaagcccctc tggtgatttt
    14161 ttgatttctg taagtaacac gtgccatctc cctttatagt atatgtcata ttatatcaag
    14221 ttttacaggc tttgtcttaa aaaaacaaaa cgcaaggcat agctacccta cgttttgtta
    14281 acgactacat atgacgcaaa cgctcaatac gatctgcaat tggtggatgc gtataaaata
    14341 aagactttaa cccttctttt ttcgttgggt cattgatata gagagccgca ctagcatcat
    14401 ctactggatg ccccataggt tcagaacgat caagtttttc taaagcagag atcattcctt
    14461 gtggatttct tgttaactca acagaactag cgtctgctaa gtattcgcgc tgtcttgaaa
    14521 tagctaattg aactaaacta gcggctaatg gtgcaaggat tagtgataaa atagagaaaa
    14581 tcaaaaccaa gatgtttcca ccatcttctc tgtcatcatc acgcctacga cctccgccgt
    14641 aaaataacat acggctacca atgcttgata ttagagttac agcactcgct aaagctactg
    14701 caatggttga aatacggata tcataattac gaatatgact aacctcatgt ccaataacac
    14761 cctctagctc ttcgcggttc ataacagcta ataggcctgt cgttgctgca acagcagcat
    14821 tttctggctt agagcctgtg gcaaaggcat ttagggagtc atcttcaaca atgaaaacgc
    14881 gtggcatagg tatctgagct atcattgcca tatcttcaac aatatgaaaa taatttggcg
    14941 cctcatcttc tgtaacttca cgcgcattgt tcattgacat aacaacatta gttgattgaa
    15001 aaatcatact tactgcatag ataaccccaa taattagagc tagaacaagc ccaaattggt
    15061 agctgcctaa taccaaataa cctacagctg ctccaatagc tgctaaaaga cagaagaaga
    15121 caattaacaa gacaactgtt tttctcttat tacttgcaat ttgttgatac agcaaaagcc
    15181 tctctccctt ctcaattaaa agtcaaagga aacttttggc acttcttttt cttcttcagg
    15241 agtttctaag aattggcttg gtttgaaccc aaatagtttt ccaacaatat tacttgggaa
    15301 agtttctaat ttcacattat aattggatgt tgttgtgtta tataactgac gtgaataaga
    15361 aattttattt tccgtattag ttagttcttc ttgaagttta acaaagctat tgttggcttt
    15421 taaatctggg taattttcag caaccgctaa aatacttgaa agctgtcttg ttagctcatt
    15481 acttgctgtc attgcttcag caggtgtgtt agctttagca acttgagcac gcagttctgc
    15541 aatcttctca agtgttttcc cttcgtacgc agcatagcct ttaacagttt cgattaggtt
    15601 tggaattaaa tcgttacgac gtttcaattg aacgtctatt tgactccatg attctttagt
    15661 atgcatgcga cttctaacta aactgttata agcaacaatc aaccaaataa caaacaaagc
    15721 aataattgct attaaaatca tagttcccat agtagtagct cctttacatt ttttccatta
    15781 tagcaatttt ctttagatta ggaaagtcct gttattccca ttattagtat ttgtgataaa
    15841 atagagtcat tatacgatat agaaattgag gtttactatg acaccccaag cattttatca
    15901 agttctgata gagcatggta ttacacttac tgacaaacag aaaaaacaat ttgaaactta
    15961 ttttagactt cttgttgaat ggaatgagaa aattaaccta acagctatta cagataagga
    16021 agaggtttac ttaaagcact tctatgattc tattgcgccg attttacaag gttatattga
    16081 caatagtcct cttagcattt tagatattgg tgctggtgca ggttttccta gtattcctat
    16141 gaaaatcctt tatcctgaaa ttgatattac tattattgat tcactgaata aacgcattaa
    16201 ttttctaaac atattagcta atgaacttga actttcagga gttcacttct tccatgggcg
    16261 cgctgaggac tttggtcaag ataaagtatt tcgagctaaa tttgatatcg taacagctcg
    16321 tgcagtagct agaatgcagg ttctagcaga attgacaata ccttttctta aagttaatgg
    16381 tcgcttgatt gcactgaaag ctgcagctgc tgaagaggaa ttaatttctg cagaaaaagc
    16441 tttgaaaaca ctattttcac aagtaactgt aaataaaaat tataagttac ctaatggtga
    16501 tgaccgaaat atcacaatcg ttagcaagaa aaaagaaacg cctaataaat accctcgtaa
    16561 agctggtact ccaaacaaga agcctttgta aagttgggag ctagtatgaa acatttcttt
    16621 gattacaaga caatgtctgt cgcacgtaaa ctcagcatta gttttatcgc tgttatccta
    16681 ttgggaagta ttttactttc acttccaatt tttcaatatg ctaacgcccc taaaacacat
    16741 tacattgatc accttttcac gactgtttca atggtatgtg taactggttt atctgttttt
    16801 cctatcagta aggtttataa cggttggggg cagattgtag ctattttact catgcaaaca
    16861 gggggacttg gtttagtaac actaatgtcg ttgtcttact ataccctaag acgtaaaatg
    16921 tcgttaaatg atcagaccct tttgcagtca gcgataactt ataacagttc gacagattta
    16981 aaaaaatatc tctatatgat ttttaaagta actttaactt tagaagtttt agcagccagc
    17041 attttagcca tagattttat tcctcgattt ggtttgagtc atggtatctt taatagtata
    17101 ttcttagctg tctcagcctt ttgtaatgcc ggttttgata atctaggggc aacgagctta
    17161 gctcaattta aactaaatcc tttggttaac attatcgttt gtttcttgat tatttcagga
    17221 ggacttggat ttgccgtatg gaaagattta attgaagcta ctatacaaac atctcacaag
    17281 ggaccaaaat taatcaagac atttccaaaa cgcctcagca accattctaa actggtttta
    17341 aaaacaacaa ccattatttt actaacagga actttgcttt cttggttgtt agagtttgga
    17401 aattttagaa caattgctaa tttatctcta cctaaacaac taatggtcag ctttttccaa
    17461 acagttacga tgcgtaccgc tggattttca actattgatt atacccaaac tgatttcgca
    17521 accaaccttg tttatattat ccaaatgtta attggaggag ctcctggtgg aacggcagga
    17581 ggatttaaag tcactgttat cgccatttta ctcttactat ttaaagccga actgagtggc
    17641 caaagccaag tgacttttca ttatcgaaca attccttcca gtattattaa acaaacacta
    17701 tcgattttaa ctttcttttt tatcatactt attagcggct atctccttct tctggaactc
    17761 aatccccata tagatccttt ttctcttttc tttgaggcat ctagtgcctt agcaaccgtg
    17821 ggtgtaacaa tgaacactac caatcaatta actctgggcg gtcgaattgt aattatgttc
    17881 ctcatgttta ttggtcgagt cggtccaatt accgtactgc tatcaattct tcagaaaaaa
    17941 gagaaggaaa ttcactatgc tgaaacagaa attattctag gttagaaaag gtcttttatg
    18001 aaaacaaaaa ttattggtgt acttggtcta ggaatatttg gacaaacact cgcacaagaa
    18061 ctaagtaact ttgaacaaga tgttattgct attgacagca atcctgaaaa tgtacaagct
    18121 gtcgccgaag ttgttacaaa agcagctatc ggagacatta ctgatttagc tttcctaaaa
    18181 cacatcggga tcagtgactg tgatactgtt attattgcta caggaaacag tttagagagc
    18241 tcagtattgg ccgtaatgca ctgtaaaaag ttaggcgtcc cacaagttat tgctaaagct
    18301 cgaaaccttg tatacgaaga agtactttat gaaattggtg ctgatttggt tatctctccg
    18361 gagcgagaat ctgggcaaaa tgttgctgca aacctcatga gaaataaaat tacagatgtc
    18421 ttccagattg aatctgatat ttctgtcatt gaatttaaaa ttcccaaatc atgggtaggg
    18481 aaaactgtcg aacaattaaa tatccgtcac aagtttgatc ttaacctcat aggtatcaga
    18541 aaagcaaaaa acaaacctgt cgataccgaa gttccaatca atactccact tgaagaaggt
    18601 attattctag tggctatcgc taactcagat gccttccaac gctatgacta tttaggatac
    18661 tttaactaaa gaaaataaaa aactgactat taaagtcagt tttttattgc catgggttat
    18721 agaaacgtcc atgattaagg tgaattaaat aaaggcttac aaacagacta gctagagcaa
    18781 gtatgattaa gacaatgtca cttttttcca aagattgctt agagtaccaa gtacgtcttt
    18841 tatttttacc aaatcgtcga agttccatag cagttgctac tgtatcaata cgttctagag
    18901 agctaaaaat taatggtgtg acaatctgca aattcccctt aatgcgtgct actaagttcg
    18961 atttttttga taattctatt cctcttgcct cttgtgctcg acgtattgta taaaattctt
    19021 cttgaacatc aggtatatat ctaagggtca aactaacagc ataagctatt ttatatgata
    19081 aacctaattg atttaaactt gatgcgaact gactaggatt agttgtcatc aaaaacaaaa
    19141 gagctaaagg cactgtacaa aagtatttta aaatcagatt aaagaggtaa aacaactctt
    19201 ggctagtcac atcatatggg ccaattcctt ttaacaataa agaagaacta ccataaatct
    19261 tatcaccata atggggagca aaaagataga ccattataat atttaaaaca gcgaatacaa
    19321 caactaaagt cataacaaag gacacctgtt gccatttgat ttttgctaat ctaaataaga
    19381 ctagcgatcc caatgaaaca acaattaaaa atcttgtatc ataagtggtc atgcaagcaa
    19441 cagaaactaa aataaaaaat agtaacttac tggcacctga tagcctgtat agaaagccat
    19501 aaccctcttg ataaccaatt aattgtttag ccattcttac ctccttgatt atcaatgtac
    19561 cattgtgtta gtttctgtgg agatatccct aatctgtctg ctaaagcaaa taaactcgtt
    19621 ttctttaaat gagtcttttt caaaagctct gtatttgaaa ggatgttaat aggatgatca
    19681 tctgcaacaa tcagaccatt gtcgattaca attgcacgat ctgtatactc taacataagc
    19741 tgcatatcat gtgtaatcat aacaatggta tgtccatcac aacttagttt atcaaggaaa
    19801 gacatcattt cagtataatg cttcatatct tgaccagctg ttggttcatc caaaataata
    19861 acttctggat ttaaaatcag tatcgaagca atcgtaacac gttttttctg accgaatgat
    19921 agagccgata taggccaatt cctaaattgg tagagcccac aaacctttaa tatatcgtag
    19981 acacgacttt ctatatcatt atcactaaaa cctcttaaac ggagacccag agcaacttca
    20041 tcaaaaacca tcgcttgact tatcatctga ttgggatttt gcaaaacata gccaattcgt
    20101 tctgctcttt cttttataga gtcttgactg acatcttcat tattataaat tagagtagca
    20161 ttattaggga taaattgaca taacgcttta gctaaagtag atttacctgc accatttttc
    20221 ccaacgatag ctaaccgctg acctttatat aaatcaaacg aaacatcatc taaagtatta
    20281 ttctctaaat catatgacac cgacaaatgc tgtactgata ataatttatc tgtatgttta
    20341 tctaaatcta atgctcgcat tttaggtatc aatagttcac tgaaatcaaa gtcatcaata
    20401 gacgtcattg tattttgttt ttcaaagtca tatcctaaat ttttgagagc agaaatatac
    20461 agtggttctc gaataccata ttcagataaa aaattagttt ttaaaagttg atccggctcc
    20521 ccattataaa taatttgtcc atcactaaaa agaactacac gatctactcg ctcataaaga
    20581 acatcttcca atcgatgttc aataataata gtggtcgcat cttgctcttg atgaatacgg
    20641 tcaaccaatg ccatgatatc ttgtcccgat tgtggatcta gattagccaa aggctcatca
    20701 aataatagaa ttgggctatc gtctactaaa actccagcta aactaacccg ttgtttttgt
    20761 cctcccgaca agtcttgtgg acgtttagac aaaagaggag ctatttctaa acgttttgcc
    20821 cacatttcaa cgatagaggc catttcttcc tgtgctacaa catcattttc taatgcaaaa
    20881 gcaatatctt ctgctactgt caatcctata aattggccat ctggatcttg taaaaccgta
    20941 gaaactaaat gagacttgtc atatattgac aagtcaaaag catttttatg gttaatcgta
    21001 aaaataccac tatattggcc tttatgagta tttgggataa taccatttaa acaatgacca
    21061 agcgttgact tccccgaccc tgaaggtcct aatatcaaga ccttttcacc tttaggaatg
    21121 cttaagttaa tcccttttaa agttggttca gattgaacgt catattgaaa ggtaaaatcc
    21181 ttccattcaa taaaatcttt catagattac tctcttatcc taatctttgg ataaactacc
    21241 tttttgtggt cgtgatttag cgtatgctag caacaaaagc gtaccaccta ctccaatagt
    21301 aatggaattc acaaggcttg ataagaaacc ttgtgcaaaa actttgcttg caggttcgct
    21361 ataaaagaaa atatcaccat acggtgcaac gacagaccaa cctataaggt tagcaataac
    21421 ttgaactaca ttaaatagaa gcaaatcctt cttaatcatg ccagataatt gaatagctaa
    21481 ttttttagca aaaaagccta tcatcaaacc taaaagacca ctaactaaaa cccaagtcca
    21541 ccacggagaa ccatattgaa tcgaatcttt caatgcgtgt ccgataaaac cagtaaaaaa
    21601 accaactcct ggtccataaa taactgcaaa taatgctaaa actgcatact gcaattgaat
    21661 attagtattt ggaattggtg ttggtatatt aactagcata cctataatga taaaaagtgc
    21721 agctccaatt ccagtcgcta caaccttttt tattgtgttt gtattcataa aaacctctct
    21781 gaaaaacaag cttaaacagt cacttacaat ctgttttatt ttatcatatt tcaagctgat
    21841 ttgaaacctt attgacaaga agaactgaaa aacgattgaa aataatatta aataccaaaa
    21901 aagatctcag atagaatacc taagaccttc attagtttat ttttttccaa aaactgattt
    21961 ttcagctaca gccgtgaaca gaacgtctgt cgatgaattt aaagctgtct cacacgaatc
    22021 ctgtatcacc cctacaatga aaccaactcc tacgacttgc attgcaacgt catttgaaat
    22081 accaaataag ctacatgcca ccggaatcaa taaaagtgaa ccaccagtta caccagaagc
    22141 tccacatgct gatacagcag caaccacact taataagaat gctgttggaa aatcaacagt
    22201 aattcctaaa gtatttactg ctgctaaagt caggatatta attgtaatag ctgcaccagc
    22261 catattgata gctgcaccaa gcgggataga tactgagtaa gtgtctttat ctaatcctaa
    22321 atcctcacat agtctcatat taacaggaat attcgccgca gaactacgtg taaagaaagc
    22381 tgtaatacct gaatccttta agcatcgtaa taccaaaggg taaggatttt tacgcatcat
    22441 aacaaaagca atgattgggt taacgacaag agctacaaat aacatcgtac ccaccaaaac
    22501 taatattaaa agaccataat cacctagtat accaataccg ttttctgaaa tgctagtgaa
    22561 aaccaacccc ataataccaa ttggggccaa attgataatc catttgacaa cttgtgaagt
    22621 agcatcagca aaagtttgca ttaaatcttt tgtacgctga ttactgcttc gcatcgccaa
    22681 accaaaaata accgcccaaa atagaatact catataattg gcctcggtaa tggcatgaat
    22741 gggattatca acaattttca acaaaagaga ttgaaagact tctgaaatcc ccttgggagg
    22801 taataagtct gtctttgctg gcgttttcaa tatcagtgtc aaagggaaaa tatagttcac
    22861 aacaacagcg attagggccg ctgcaaaagt tgcagtgata tacaaaccaa ttatcgttga
    22921 catattggtc ttaccgccct cacggtgttg cgacaaggca ctaataacta aagtgaaaac
    22981 taaaagaggg gcaatagctt tcaaccctcc tacaaaaagt tgaccaagta aaccaatcgc
    23041 agaggcttta gggactaaaa tacccaagaa taagccaata acgactccaa ttgttatttt
    23101 ttttatcaag cttgtttttc tccaagcttt taaaaatcgt ttcatactaa actcctaaaa
    23161 aattcttaaa gtctatgata tgataaaaaa aaagattcgt caaatacctt ttttattttt
    23221 tagaggttga cgaattctaa atttttctga tataattact ttttgaaata ttcaatcaat
    23281 ttcgaaaata aaatattttt ttgtacagaa aggtacattg atgaaactaa aaaacactta
    23341 tatcattatc ggcttgatgc tttttgcgct gttctttgga gccggtaatc ttatttatcc
    23401 agcctactta ggtatctact ctggttctaa ttgggcgtgg gctatcattg ggttctgctt
    23461 aacaggcgta actttacccc tacttggtgt tgcagctgtg acgtactcag gagcaaccga
    23521 tgttgaaagc ttcaccaaac ctgtctctaa aacatatgct attttctttg cagtagcact
    23581 atacctatct attggacctt tctttgccat tcctcgaaca ggggcaacct cattctcagt
    23641 tggtattcaa cctatcttag gcgacagctc cttagtcaaa attatctatg gtttaatttt
    23701 ctttggtatt tcttatttct tagctattag acctagtcag attgtagatc gtattggtaa
    23761 atatctaact ccctttttac taactgttat tagtatttta attattgcct cactcatcca
    23821 tccagctgct atcgttggaa caccacataa cgcttcaacg aatgtcagcg atgctttcaa
    23881 ggatttacca ttcctagcag gactgattca ggggtatggt acaatggatg cccttgcatc
    23941 aattgctttt gctattatcg ttattcaagc aagtaaacag tacggagcaa ttaccaaaaa
    24001 agaaattact tctatgacac ttaaatctgg tgctatcgct acatttcttt tagcttttat
    24061 ttatattttt gtaggacgta ttggtgcaac ttcacaaagc ttgtttaaat ttgcaaatgg
    24121 ttccttctta cttcacaata ctccaataga cggtgggcat gttcttagtc aatcagctaa
    24181 cttctattta ggaattgtgg gacaagctat tttaggtacg gctatttttc ttgcttgctt
    24241 aacaacagct actggattaa tcactgcttg tgcggaatac ttccacaaat tacttccaaa
    24301 gattagtcac attacttggg ctacaatctt tactttaatt gctattacgt tctattttgg
    24361 tggtttatca gagattatcc gttggtcact accagttctc taccttctct atccattaac
    24421 tatagtctta attttcttag tcttttttga tcaaaaattt gaaagtagcc gcattgtcta
    24481 ccaaacttct attgccgcca ctgctgtcgc tgctttatac gatgccttat caaaactcgg
    24541 cgaaatgact ggtttattta ccattccatc cgctttgacc acattcttta ctaaagttgt
    24601 tcctttaggt gaatattcta tgggatggat tagttttgca atttgtggtg tcttagttgg
    24661 tcttattcta aaaaaagtaa aagcataata atttaaagca gtacgaaaat tactgctttt
    24721 attttaaaat aattattgta accgctttct tttggtggta taatagcctt gaaaataaat
    24781 aatttttgga ggaactctaa tatgagagct gcaacttatc tttcagctaa tgaattatca
    24841 cttactgata aggctaaacc tcaagtaata aaacccacag atgcggttgt aagacttgtc
    24901 aaaacaacca tttgtggtac tgatctacat atccttggtg gcgatgttcc tgcttgtaaa
    24961 ccaggaacaa ctttaggtca tgaaggcatt ggtatcgtcg aagaagttgg ttctgctgtc
    25021 aatcatttta aagtcggaga taaagtaatt atttcttgtg tcactgcctg taatacttgc
    25081 tattactgta agcaccagtt gccttcacat tgtgaagatg gcgggtggat tttaggacat
    25141 cttattgaag gtactcaagc agaatatgtt catattccac atgcagatgg tagcctttat
    25201 catgcacctg aaggtgtttg tgacgatgca ttggttatgt tatctgatat tttaccaact
    25261 tcatacgaaa tcggcgtctt accttctcac attaaaccag gtgatactgt ttgtattgta
    25321 ggcgctggac caattggact atctgcctta ctaactgcac aattctattc tcctgccaaa
    25381 attattatgg ttgatttatc tcaaaaacga cttgaagcct ctaaaaaatt tggtgcaaca
    25441 cacaccattc tatcaacgtc tactcaagaa gttaaagaag aaattgataa aattaccaaa
    25501 ggccgtggcg ttgatgtcgt attagaatgt gttggttatc ctgcaacatt tgatatttgt
    25561 caaaatgtag tttctattgg aggacatatc gcaaatgttg gtgttcatgg taaacctgtt
    25621 gaatttaacc tccaagattt atggataaaa aatataaccc ttaatactgg attagttaat
    25681 gctaatacaa ctgaaatgct ccttgaagtt cttgagacag gcaaaatcga tgcaacacaa
    25741 ctagtaacac atcacttcaa gctatctgaa atcgaagaag cttataaggt atttaaagct
    25801 gctgaagaaa acaatactct caaagttatc atcgaaaacg atattactcc agcctgataa
    25861 tttttaaaca aatgaaaatg tgagaagaat agccttctca catttttatt tatgactcaa
    25921 gcgacgtgtt aaaaaatcac ctaaaaattg gataaagaaa attaataata aaatcagaat
    25981 agtcgctact aaagtaatat catcacgaga atagttatat cctttagtaa tagcaacaga
    26041 acccaatcct cctgcaccaa tagcgccagc catagctgtt tcacctacta aagaaatcaa
    26101 agtaaccgtt gatactcgaa ttaaatctgg tagaccttca cgaagataaa ctacaataat
    26161 atcccaaagt gttccacctg aggcttgtgc agcctcaata actccaccat caagttcagc
    26221 taaaacaact tgaacttgac gagcaaaaaa tgggaaaact gccaaagaaa gaggtaccaa
    26281 agctgctggt gaaccaagtg ttgttcctac aattacgcga gttactggcg caatcaaagc
    26341 aagaagaata atgaagggca gagctctaaa aacagaaaca actttatcta aaactccaaa
    26401 tactaattta ttagcaataa ctcctctagg actagttaaa acaaggaata aacctcctaa
    26461 caatcccatt aaacctccaa taaggaacga ccaaaaagtc atataaaggg tttgtacaat
    26521 agctgtctgc cagccgtaag caccttccca acccatttga tatacatttg gtaaatgtgt
    26581 ttgaatccac tctatcatgc tttaccacct tctttcaata acgttaattg aacttgcgaa
    26641 tctgtaattg cttggcaggc ttgtcttaat ttttcttcgt cacctgataa gataactacc
    26701 atttctccaa ctggaatacc atctaaaatt tcaatattcc catagaggat gttagcagtt
    26761 acttcaaatt ctttataaat ttgatttaaa agcggctcat ccgtgctatg cccagcgtat
    26821 ttcagttgaa ctaatttgct acctactggt agatttttga caacttcttg ttgctcaatc
    26881 ttaagcattg cctcatcaat cccagtagct atttttataa aatcttgtgt aagactctct
    26941 ctaggatgtg aaaaaatgtc taaaactgat ccttcttcaa tcaatttacc attttgcata
    27001 acagcaacgc gattagcaat gtctttgaca atttgcatct catgtgtaat caaaacaatc
    27061 gtaagtccca attttttatt cagatcttgg agaagggcaa gtatttgttt tgttgtttta
    27121 gggtcaagtg cagaggtaga ctcatctgaa attaagattt taggatcgtt agccaatgca
    27181 cgcgcaatgg caacacgctg tttttgccct cctgataatt gtgaaggata gttttgagca
    27241 cgatcggata atccaacaag ttctaataat ttagcaacct tagctgcttt cgcctctttt
    27301 gataaacctg aatgtttaag agcaaaagct acattctgtt ctgctgtcaa ctgtgacatt
    27361 aggttaaaat gttgaaaaat cattccaatt tcacggcgct gttcgcgtaa ttgtgtgcta
    27421 gtcagtgtca ccttgttgtc gtaaataact tggtcatcaa tagtaatttt acctgctgaa
    27481 ggttcttgta gcaaattaat aacacgaaca agggtagatt ttccggctcc tgaataaccg
    27541 acaattccat atatatcacc ttgattaata tggatagtaa catctttaac agcattaatc
    27601 tcacgtttct tttggtggaa agtaacatca atgttgtcta atttaataat tggttcttta
    27661 ctcatatgat tctattaaag cctccactaa ttcaatatgg gtgtaataat cagcaatact
    27721 aacattttca tcaacgccat ggtctcgact attggcatgt cctatgccaa tagctgcaat
    27781 tggcgctctt agagcttgat gaactaaata cattggtcct gtacctggtg aggtaggtaa
    27841 cagagatatc ccttctggat aaaattgctc agctaaatca acaaccttta ggatagatgg
    27901 cgctgacata tcactacgat aagacatttc accaagggta taggttagct caatgtcttt
    27961 aaagccattc tctttcaaat gattttgtat tgaatctagt acacctttcg gggttaaccc
    28021 tggtactaac ctaacctctg ctttacattt agcataagca ggtaaaattg ttttgacgcc
    28081 ttcaccttga taacctgagg ttataccttc aatggctata gaaggttcaa aataaagctt
    28141 tctcaaaaaa gttttatgac tatctgctaa actgggcaaa actaattggt aagcaccttc
    28201 tagcgccttt gcagatctat aagaatattt ttcaactaat tctaactctc tcttagtagg
    28261 gggaataact ttatcataaa taccttctac caatatatga cctttgttat cccttaacgt
    28321 attgagggct tgaattaaat accatgtcga ggaatcaaca accccaccaa aggatgaatg
    28381 aatatcaaca tccgctgaac ttactgataa atcaaaagtg acaatgcctt tatttccacc
    28441 agcaatttcc aactgtcctt taggattttt agggccatct tcccacacga tcaaatcagc
    28501 cccttgtagc tgctcttgat acttttctaa ataataatca aggcctactg aagctgattc
    28561 ctctgctcct tccacaataa aagtgatatc tagaggaagt tcccctttgt gtcttgataa
    28621 atatttttta actgctgata aacgtgcggt aatgtgacct ttatcatcat ctacaccacg
    28681 tccatacatc ttaccatatc gaagtgaaag tgtaaaaggg tcctcagtcc actgttctac
    28741 ctcatctgct ggtacagtat cataatgatt gtaaaaaata atacgcttag catctacttt
    28801 tgatgatttg aaatttgcca cgataaaggg agcgctatag ctgtcatcta taatgacttc
    28861 tgcgccagct tttctgaaaa cttctccaag atagcttgat aagtcattta aaccaacttt
    28921 ctgagcaaaa attgacttaa ttgcaattaa atctcttaaa atattgatat catcctgaac
    28981 aatagtatca gatagaaact tttgaatttg attttcttcg tttgttaaag tcatctttca
    29041 tccaccttcc ttcaaaaaca atccaagagc ctggcacatt gtattcgtcc caggcccttt
    29101 atggctatat ggctgataag ttgtatccta ttaggattcc ctgttaaact tatacagtca
    29161 attcacgtta ccattgtgga atatcagctg aagtatcttt gataactttt ttcacttcat
    29221 ctgtgtgata agcatccaag atagcttgga tagctttagc gttcttttgc tttttccaat
    29281 ttttacgtcc cgcaatgata ttaatccatt gttttgaatt tttatctgat ttctcaacaa
    29341 agatagcatc tgaaggtttt aaattagctt gctcaatgta tgtattatta ataatagctg
    29401 catctacatc tttgagtgca cgtggtgttt gactcgcatc taactcctga atattaatat
    29461 cctttttatt agatgtgata ttagcaactg ttgcaacctt cttaccagaa acattcaatt
    29521 tgattaaacc tgctgactga aggacataca atgcacggct accatttgtt gcatcatttg
    29581 gaattgcaat agtggctcct tttttcaatt ttttaagaga ttttaccttc tcagaataga
    29641 tacgaattgg agctaagtaa gtcttttcaa gtggaattaa gtttttctta ttttccttat
    29701 tccagttttc taagaaattg taatgttgaa aggcattaat atccacatcc ttattggctg
    29761 tcgcttgatt tggttgtgta taatctgtaa attctgtaaa tttgatttta gctttatcac
    29821 ctactagctt ttcaatttta tcccaacgtg ctttttcagt gtcagaaaag gtcataacac
    29881 caactttaac aactttactt gaagttgagc ttgctgaaac ttcttgatta ctaaatgtga
    29941 ctcctaaaag tgttgt
//
LOCUS       DATROY010000009        27059 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.009, whole genome shotgun sequence.
ACCESSION   DATROY010000009 DATROY010000000
VERSION     DATROY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27059
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            211..1233
                     /locus_tag="VBD11_000388"
     CDS             211..1233
                     /locus_tag="VBD11_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6953365.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            1604..2896
                     /locus_tag="VBD11_000389"
     CDS             1604..2896
                     /locus_tag="VBD11_000389"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6953366.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(2919..3212)
                     /locus_tag="VBD11_000390"
     CDS             complement(2919..3212)
                     /locus_tag="VBD11_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953367.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
     gene            3551..4168
                     /locus_tag="VBD11_000391"
     CDS             3551..4168
                     /locus_tag="VBD11_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953368.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            4336..5775
                     /locus_tag="VBD11_000392"
     CDS             4336..5775
                     /locus_tag="VBD11_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6953369.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            5803..6081
                     /locus_tag="VBD11_000393"
     CDS             5803..6081
                     /locus_tag="VBD11_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6953370.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            6148..6633
                     /locus_tag="VBD11_000394"
     CDS             6148..6633
                     /locus_tag="VBD11_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6953371.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            6746..7411
                     /locus_tag="VBD11_000395"
     CDS             6746..7411
                     /locus_tag="VBD11_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6953372.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            7415..8278
                     /locus_tag="VBD11_000396"
     CDS             7415..8278
                     /locus_tag="VBD11_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6953373.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            8280..8996
                     /locus_tag="VBD11_000397"
     CDS             8280..8996
                     /locus_tag="VBD11_000397"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6953374.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            9007..9654
                     /gene="fsa"
                     /locus_tag="VBD11_000398"
     CDS             9007..9654
                     /gene="fsa"
                     /locus_tag="VBD11_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6953375.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            9817..10800
                     /locus_tag="VBD11_000399"
     CDS             9817..10800
                     /locus_tag="VBD11_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6953376.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVRYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            11035..12054
                     /locus_tag="VBD11_000400"
     CDS             11035..12054
                     /locus_tag="VBD11_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953377.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            12073..13029
                     /locus_tag="VBD11_000401"
     CDS             12073..13029
                     /locus_tag="VBD11_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6953378.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            13127..14578
                     /locus_tag="VBD11_000402"
     CDS             13127..14578
                     /locus_tag="VBD11_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6953379.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            14600..15589
                     /locus_tag="VBD11_000403"
     CDS             14600..15589
                     /locus_tag="VBD11_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6953380.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            15655..17172
                     /locus_tag="VBD11_000404"
     CDS             15655..17172
                     /locus_tag="VBD11_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6953381.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            17192..17953
                     /locus_tag="VBD11_000405"
     CDS             17192..17953
                     /locus_tag="VBD11_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6953382.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            18019..19698
                     /locus_tag="VBD11_000406"
     CDS             18019..19698
                     /locus_tag="VBD11_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6953383.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            20015..21106
                     /gene="ulaG"
                     /locus_tag="VBD11_000407"
     CDS             20015..21106
                     /gene="ulaG"
                     /locus_tag="VBD11_000407"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6953384.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            21196..23574
                     /locus_tag="VBD11_000408"
     CDS             21196..23574
                     /locus_tag="VBD11_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6953385.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            23951..25174
                     /locus_tag="VBD11_000409"
     CDS             23951..25174
                     /locus_tag="VBD11_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6953386.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            25193..26920
                     /locus_tag="VBD11_000410"
     CDS             25193..26920
                     /locus_tag="VBD11_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6953387.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         8501 a         4425 c         5438 g         8695 t
ORIGIN      
        1 gcttttaggt aaaatgtgat tatattagca actaaggagt aatccatgag ataagcctaa
       61 taattatgtc ggacttcttt gttgcacctt atccagttcg tagtacaatt aagtttcagt
      121 attacctaaa ggcgccttta ttgaaattga agcagtagta cacagacact gagaataaaa
      181 aacttaacta tttttagcgg gagtatattt atgaatatca ttattgggac cagtctttta
      241 atacttgtat tagcgatttt cactttattt aattataaag caccctacgg aacaaaagca
      301 atgggagcat tggcatctgc ggcttgtgca tccttccttg tagaagcttt tcaagattct
      361 ttctttggta aagttttggg atttcaattt ttaagtgaag tgggtggtgc taatggttca
      421 ctttctggtg tagctgcagc tatattggtt actattgcta ttggtgtaac cccaggatac
      481 gcagttttaa ttggtttatc tgtttctgga acaggtatta ttcctggttt ccttgcagga
      541 tatttagtgg gatttcttgt taagtggatg gaaagaaata ttcctggtgg tttagatctt
      601 atttcaatta ttattattgg tgcaccatta acacgtcttg tggccaaact gttgacgccg
      661 cttattaata gcactttgct caccattgga gatatcttaa catcaggtgc tcatagcaac
      721 cctattttaa tgggggttat acttggcggg acaattgtag tagtggcgac agcaccactt
      781 tcttctatgg cattgacagc tatgctagga ttaaccggaa tgcctatggc tataggagcc
      841 ttgtctgtct ttggttcgtc atttatgaat ggtgtacttt tccataaatt aaaacttgga
      901 agtcgtaaag ataatatagc ttttgctgtt gagcctctaa ctcaagctga cgtgacttca
      961 gctaacccta ttccaatcta tgtcactaat tttgttggtg gtgcagcttg tggtatttta
     1021 attgccttga tgaaattagt taatgatact cctggaacag cgacaccaat tgcaggattt
     1081 gctgtcatgt ttgcctataa cccaatgata aaagtactaa taaccgctct aggttgtatt
     1141 atcctatctt tactagcagg ctattttgga ggcattgttt ttaaagatta taaattagtt
     1201 acaaaagaag agctacaggc tagagataac tagttacttc atgaaaagct aatagtaaat
     1261 atcattaata tagatatgat atctgacttg tgataactgt tattgataat ttaaaattca
     1321 acgactctaa taaaaagatt gttgaatttt tttattgtta agaatgctgg tatcagtaaa
     1381 tttcaagatt tctttatcaa ctataatgtt agttttttac cgttttttta cacttctaat
     1441 aattattttt ttcttttttt ccgataatat ttgaggaatt tggcaaaaaa aaacattttt
     1501 tgctataata aggtgtcaga caaactcgca ggtgaaattc ctgccatgtt tttccagaaa
     1561 ggattgggga gtcccaaaaa ggctcactca gtgttaaata aatatgactt cagttgttgt
     1621 agtaggtacc caatggggtg acgaaggtaa aggtaaaatt acagattttt tatctgctga
     1681 cgcagaagta attgcacgtt atcaaggtgg tgataacgct gggcatacta tcgttattga
     1741 taacaaaaaa tttaaacttc atttaattcc ttcaggaatt ttcttcaaag aaaaaatttc
     1801 tgtcattggt aatggtgttg tcgttaatcc gaaatcatta gttaaagagt tagcttatct
     1861 tcacggagaa ggtgtaacaa cggataacct tcgtatttca gatcgtgcgc atgttatctt
     1921 gccataccac atcaagcttg accaactaca agaagatgct aaaggtgata ataaaattgg
     1981 aacaaccatc aaaggaattg ggccagctta catggacaag gctgcgcgtg tcggtatccg
     2041 tattgctgac cttcttgata gagaagtttt tgctgagcgt ttgaaaatta atttagctga
     2101 gaaaaatcgt ttatttgaaa aaatgtatga cagtacacct cttgaatttg atgatatttt
     2161 cgaagaatat tatgaatatg gtcaacaaat taagcaatat gtgactgata catccgttat
     2221 cttaaatgat gctcttgatg ccggtaaacg tgttcttttt gaaggagcac aaggagttat
     2281 gctcgacatt gatcaaggaa catacccatt tgtaacatct tccaatccag tagcaggtgg
     2341 tgtcacaatt ggttcgggag ttggaccaag taaaattaat aaagtggtag gtgtatgtaa
     2401 agcttacact agccgtgttg gcgatggacc attcccaaca gaactttttg atgaggttgg
     2461 tgaccgtatt cgtgagattg gtaaagagta tggtacaacg actggtcgtc ctcgtcgcgt
     2521 tggatggttt gattctgttg ttatgcgtca cagccgtcga gtatcaggta ttactaacct
     2581 ctctctgaat tcaattgatg ttctttcagg gcttgatacg gtgaaaattt gtgtggctta
     2641 tgaccttgat gggaaacgta ttgactatta cccagcaagc cttgaacagc taaaacgttg
     2701 taaaccaatc tatgaagaat taccgggctg gtctgaagat attacagctt gccgtagctt
     2761 agatgatctt ccagaaaatg cacgtaatta cgttcgccgt gttggcgaat tggttggtgt
     2821 tcgtatttct actttctcag tagggcctgg tcgtgaacaa acaaacattc ttgagtctgt
     2881 ttggtcaaat atttaatatt aaaaatctga ctgtacagtc agactttttg atgtttactt
     2941 tttcttttta atagatagat actaataaga cttaaggact tcccagaaat caataaaact
     3001 actataattg ataaaaaaag aagtgaattg atattgtgaa tcaaagtatg taataatact
     3061 agcgatacaa ctgttagaat actaccagtc atcaataagc gttccagagg atagtagcca
     3121 tgttgcttat gcatttctaa ttttaatagt agaccagctg acaattctgc taggaagcaa
     3181 ataggtagag ctatcaaatt aataagaatc atagaccaac caaatccttg accataacct
     3241 atatatgata gactattttt gggaattgtt gacgtgtgaa ttataaaatc aatcgtaaag
     3301 ataatgtcta taagaagtag tagcgctaac agataatgta gtaagaagat tgttcttaac
     3361 cgtttattaa tcaatatttc atttcctcct acttatttac caataagctt attatatcat
     3421 aatgtataat caaacattcg ttagtactga ttaaaaggtt tatggagggg cggttactat
     3481 tgtttcattg aattagtaac tttaacgacg aaatgttata atgttattag tttataaaag
     3541 aggagattgt atgcgtaaat ttttccaaga tgttattgta gaggtggctt attatttaga
     3601 gattgctttg tcagttattc tggcaacagc tattattttc tttagtttag cactctttca
     3661 tgatttgttt agcatgttgt ctgttgatca aaatgttgat aagttgttcc agactttcct
     3721 tgctcgcgcc atgacaatag cagtcgggat tgagttgatc aaaatgtttt caaaaccttc
     3781 ccctgatact ttaattgaag tgttattgtt tgccttagct cgtcaacttg taattgaaca
     3841 tggtagtgtt tcagattacc ttatggggat tgcttcagtt gctattttgt ttgctgttcg
     3901 taaatatctt ttcacccatt ttgatgagtc aagcaacatt gttttacgtg ctaataacaa
     3961 agtcaaaaaa gctaatattt tagctagggt tcaactacct tgtcaaccca atgaaacttt
     4021 aggagactta cttgttcgtc atttaaatga tgaggaaaag gacgttgcta ttggttcagt
     4081 agtctatatt aataacgttg cattgcgtgt tgatagtatg cacgatggtc ttgtgacgcg
     4141 tgtagaaatc attaggagtc tacattaaaa aatataaaaa atcatgtatc ttaggataca
     4201 tgattttttt gtaaattagg ttcagcacaa ttttgcacac ttaagtgtgt acttcttggt
     4261 cttttttaaa acgcttacaa aaaatataat aaagatacaa aaaaacatta cttaaataaa
     4321 gagaggtctt tagttatgga aaacttccta gctcccctta attggttctc gcaaaatatc
     4381 ttgcagaatc cagccttctt cgtaggtctg ttggtactca ttgggtatct tttattgaag
     4441 aaaccacttc atgatgtctt tgctggtttt atcaaagcga ccgttggata tttgattttg
     4501 aacgtaggtg caggaggatt ggttaataca ttccgtccaa tcttagttgc tcttgctaaa
     4561 aaatttcatt tagaagctgc tgttattgac ccttactttg gtttagcatc agcaaatgct
     4621 aagttagaaa caatgggctt tatcagtgtt gcaacaacag cactcttgat tggttttggg
     4681 attaacattc tcttggttgc tcttcgtaaa gtaactaaag tccgtactct ttttattact
     4741 ggtcatatca tggtacaaca agctgcaaca atttcagtat ttgtactgct cttgattcca
     4801 caacttcgta atggttttgg tgcatgggct gttggtatca tttgtggtct atattgggcg
     4861 gtaagttcta atatgaccgt tgaagcaact caacgcctta caggtggtgg cggatttgcc
     4921 atcggacatc aacaacaatt tgcaatttgg tttgttgata aagttgcgcc atttttcggt
     4981 aaaaaagaag aaaatcttga taacttaaaa ttaccaacat tcttaaatat tttccatgat
     5041 acagttgttg catcagcaac attgatgcta gttttctttg gaggaatctt agctgtcctt
     5101 ggtccagaca ttatgtctaa tgttaaatta atcggacctg gtgcatttgt accaactaag
     5161 caagctttct tcatgtacat ccttcaaaca tcattgacat tctcagttta ccttttcatt
     5221 ttgatgcaag gtgtacgcat gttcgttaca gaattgacaa atgccttcca aggtatctca
     5281 aacaaacttc ttccaggatc attcccagcg gttgacgttg cagcttcata tggttttggt
     5341 tcttcaaatg cagttctttc aggatttgct tttggtttga ttggtcaatt aattacaatc
     5401 gctctacttg ttgtcttcaa aaatccaatc ttgattatca caggattcgt tccagtattc
     5461 tttgataatg cggcaatcgc tgtttatgca gataaacgtg gtggctggaa agcagcagta
     5521 gctctatcat ttatctcagg tattattcag gtggcacttg gtgctgttgc tgtaggtctt
     5581 ttaggacttg caggtggtta tcatggaaat atcgactttg aattcccatg gttagcattt
     5641 ggttacatct tcaagtattt aggtattgct ggttacgtga ttgtatgtct cttcttctta
     5701 gctattccac aacttcaatt tatgaaatct aaagataaag aagcatacta tcgtggtgat
     5761 gcgcctgcag aataatagaa atttataacg gaggaaataa aaatggttaa agttcttaca
     5821 gcatgcggaa acggcatggg atcatcaatg gttatcaaaa tgaaagttga aaatgcgctt
     5881 cgtcaattag gcgttagcaa ctttgagtca gcatcttgct cagtcggaga agcaaaaggt
     5941 ttagctgcga actatgatat cgtggttgct tcaaaccacc ttatccatga attagacgga
     6001 cgtacaaaag gtcatttagt aggtcttgac aaccttatgg atgacaatga aataaaaaca
     6061 aaactacaag aaattttgta agttaataga caaagggagt gagacaaaag tttctctccc
     6121 atatctttag acttttaagg agaaatcatg aatttaaaac aagcctttat cgaaaatgac
     6181 tcaattcgtc ttaaattatc tgcaagcgat tggaaagaag caatcaaatt atcaattgat
     6241 ccattaattg aaagtggtgc tgttgatgca gaatattacg atgccattat tgaatctaca
     6301 gaggaatttg gtccgtacta cattcttatg ccgggtatgg cgatgcctca tgctcgtcca
     6361 gaagcaggcg taaaacgtga tgcattttca ttaattacat taacagagcc agtagtcttt
     6421 ccagatggca aagaagtctc agtattgtta gcacttgctg caacaagttc agctattcat
     6481 acttctgttg ctatccctca aattattgcc ctttttgaat tggaaaattc aattcagcgc
     6541 ttgacggaat gccaagaagc aaaagaagtc cttgcaatgg tagaggaatc aaaaaacagc
     6601 ccatatttag aaggtttaga tttagaaagc taatcaataa actgatatta atagaaagga
     6661 taggacttat aaaaaatctc tgtgataaga aactctttgc cacaaataaa gtgagtattt
     6721 ttatcaggtc cttagaataa acaacatgac aaaacgttta ccaaatttac aagttgcact
     6781 tgaccactca gatttacaag gagctatcaa agctgccgta tcagtaggac atgaagttga
     6841 tgttattgaa gctggaactg tttgccttct tcaagtaggt agtgaattag ttgaggtcct
     6901 tcgcagtctc ttccctgaca aaataattgt tgctgataca aaatgtgctg atgctggtgg
     6961 gactgttgct aaaaataatg ctgttcgcgg tgcagactgg atgacttgta tctgttgtgc
     7021 tactattcca acaatggaag cagccttaaa agctatcaaa gaagaacgcg gcgatcgtgg
     7081 agaaattcaa atcgaactat atggtgattg gacttatgag caagcgcaac aatggttgga
     7141 tgcaggtatt tcacaagcca tttatcacca atctcgtgat gcgcttcttg ctggcgaaac
     7201 atggggagaa aaagatctta ataaggttaa aaaattgatt gatatgggct tccgagtttc
     7261 tgtaactggt ggtttatcaa ctgatactct tcaattattt gaaggtgttg atgtctttac
     7321 ttttattgcg ggtcgtggta ttacagaggc agatgatcca gccgcagcag cacgtgcatt
     7381 taaggatgaa attaaacgta tttgggggta atgtatgaca cgtccaatag gaatttacga
     7441 gaaggcaaca ccaaaacatt ttaactggtt agaacgccta caatttgcta aagaattggg
     7501 atttgatttt gtagaattat ccattgatga gtcagatgaa cgtttggcac gtttggagtg
     7561 gtcaaaagaa gaacgccttg agttggtcaa agctattttt gagacaggag ttcgtgttcc
     7621 aacaattaca tttagtggtc atcgtcgttt cccaatggga tctaatgatc ctgagaaaga
     7681 agcgcgtgcg atggatatga tgaaaaaatg tatcgtcttt gctcaagata ttggtattcg
     7741 taatattcaa ttagcaggat atgatgtgta ctatgaagaa aaatctccag agacacgtgc
     7801 acgctttatt aagaatcttc gtcaagcttg tacttgggcc gaagaagcac aagttattct
     7861 gtctattgag attatggatg acccatttat gaattccatt gagaaatatt tggctgtgga
     7921 aaaagagatt gattctcctt atctctttgt ctacccagat actggaaatg tatcagcatg
     7981 gcataatgat ctttggagtg aattttataa tggacatcgc tcaattgcag cgcttcatat
     8041 taaagatacc tatgctgtta ccgaaacgtc taaaggtcaa ttccgtgatg ttccatttgg
     8101 tcaagggtgt gttgattggg aagaaatgtt tgctgtcatt aagaaaacaa attataatgg
     8161 tccattcttg attgaaatgt ggtcggagaa ctgtgagact gtagaggaaa cacgtgcagc
     8221 cattaaagag gcgcaagatt ttctttaccc attaatggag aaagcaggag tgaaataata
     8281 tggcaaaatc attgcaagaa atgcgtgaac gcgtgtgtga agctaataaa tcactaccag
     8341 ttcatggttt agttaaattt acttggggta acgtttctga agttgaccgc gaagcaggat
     8401 tgattgttat caaaccttca ggagttgact atgaccaatt gacacctgaa aatatggttg
     8461 tcacagactt agagggaaat atagtggagg gggatttgaa tccatcatct gatcttccaa
     8521 ctcatgttca actttataaa gcatggccag aagtcggtgg tattgttcat actcattcaa
     8581 cagaagcagt tggctgggca caagcgggtc gcgatattcc attttatgga acaacacatg
     8641 cagattattt ttatgggcca gtaccatgtg ctaggtcact tagtgaagat gaagttaaca
     8701 ctgcttatga aaaagaaaca ggtagtgtta ttatagagga atttgaacgt cggaatttag
     8761 atccaatggc tgttcctggt attgttgtac gtaatcatgg accatttact tgggggaaag
     8821 atccagcaca agcagtttat cactcagtcg ttcttgaaga agttgccaag atgaatcgtt
     8881 tcactgaaca aattaatcca cgtgttgaac cagctccaaa atatattatg gataaacact
     8941 atttacgtaa gcatggacca aatgcttatt atggtcaaaa aggacaagaa cattaaggag
     9001 aataatatga aatacttttt agatactgcc gatgtttctg aaattcgtcg tcttaatcgt
     9061 ttaggaatcg ttgatggtgt tactaccaat cctactatta tttcacgtga ggggcgtgac
     9121 tttaaagaag ttattaacga aatttgccaa atcgttgatg gtccagttag tgcagaagtg
     9181 acaggtttaa cttgtgatga aatggttacc gaagctagag aaatcgctaa gtggtcacca
     9241 aatgttgttg ttaaaattcc aatgactgaa gaaggtttag cagcagtttc acaattatca
     9301 aaagaaggta taaagacaaa tgtgacatta atatttactg tggctcaagg gctttcggca
     9361 atgaaagctg gtgcaacctt tattagtcct tttgtgggtc gtcttgaaga tattggaaca
     9421 gatgcttatg ctttgattcg tgaccttcgt cacattattg atttttatgg tttccaatct
     9481 gagattattg ctgctagcat tcgtggattg gcgcatgtgg agggcgtcgc taaatgcggt
     9541 gcgcacattg ccactattcc agataaaaca tttgcatcac tatttactca cccgcttact
     9601 gataaaggga ttgaaacttt cttaaaagat tgggatagtt tcaagaagaa gtagtgttac
     9661 gataaagcaa actcttaatt atactagtga aagacaaact ttgataatgg gtttgtcttt
     9721 tttataatct tctccaataa gaacacttat tttcattttt cttattaaat gatataatac
     9781 ggataaatta agtctaatta gaataaggaa atagagatgt ttgagaaggt aaccattagt
     9841 gatattgcaa atttagtagg tgtgtcaaaa gctacagttt cctattatct caatgggaac
     9901 tataagaaaa tgtctctgca aacgaaagaa aaaatccgtt tagctattaa ggagactggt
     9961 tatcaaccaa gtaagattgc tcaaagttta gttacaaaaa atacccgtac tattggggtc
    10021 gttattgctg atattaccaa tccatttatt tcgtctgtga tgaagggaat acatgatact
    10081 tgtcaacaat ttggttactc ggttaacttt actaactcgg ataatgatat tgacattgag
    10141 ttggaaaatt taaaccgttt aaatcagcaa aacgtttcag gaattatttt agattctgta
    10201 gatcctaatc attcatttat agaaactttg agtaatgatc gtcttgtcat ggttgataga
    10261 caagctaaag atattaaggt agatactgta gtatccgaca ataaagaatc gactcaaata
    10321 tttcttgaaa aaatgcaaga aactgggtat catgacatct attttgtcac ttatcccatt
    10381 gaagggattt caacccgtga gcttcgttac gaaggtttta aagaagtggt cagttcaaat
    10441 cctgataagt tgattattat cactgaagat ggtagcacac agagaattct tgatattatt
    10501 gaacattcag agcaaaaacc agggttctta atgatgaacg gtccaactct tttgaatttt
    10561 atgaaaaaat taaatcaatc aacggtcaga taccctgagg attatggtct tgggagttac
    10621 gaagatttag aatggatgca ggtgcttaca ccaaacgttt cctgtataaa acaggattca
    10681 tatggcattg gttgtctggc tgcccaatgt ttaattgaaa aaattagtca aggaaacgaa
    10741 ccaactactg caaggttact agaagtaaaa aatcaaatcg ttattcgtca atctttttaa
    10801 aaatgtttgc gagagcaagc atttttttat aaaaaagcaa aaaacactat gtaaacgctt
    10861 gcttttataa aaaatgatag tataatagaa ctataaatga gttaaccgtt taacttaaga
    10921 ataggtaagc ttttaacgaa ccatctctaa ctctcttaac tcaaatgttt ttattttttt
    10981 gcaccattag ttaaacggtt aactcaagtt ttatcttcac caaaggagga tagaatgaag
    11041 attaataaat tattgtcaga cagacttgaa gcttgttatt cagtttgtcg tttgcaaaat
    11101 caagactatg attttttatt agtagcttca gaggttgatc aagcctgctt tgcttatgat
    11161 ttgaatagcg atttagcaaa aattgttgtt tgggaagatg tgggaggtac catgagtatt
    11221 attccaattc ccggaaccat ggattttttg gcaacccaga aattttaccc agggtttaag
    11281 gccaaagatt gtcaggttgt ttatgcaagt tttcaaggag atcattggga catttgtaac
    11341 gcatttgatt ttccgtattt acatcgcttt gatctcatta aaggtaaaga aaatcaactt
    11401 tactttatag gttgtacaat tgctaacagt aaagcctaca ctgaggattg gagtgataaa
    11461 ggccgaattt ttgttgctcg ttttaatact caaaaccata cattggaagg attgcaacaa
    11521 ttgcctcaaa ctttattaaa aaatcatgga tactatgcca ttcaggatga aggatattca
    11581 ttgattactt cagtagaagg ggtactcaaa ctcacttatc cagaattttc tactacaggc
    11641 gactggcaat tagaacggct tttcgatgag gagacaagcg atgtggtgaa agtggatatt
    11701 aatcaggatg gtaaggatga gtatgtgatc atccaaggtt ttcatggaga tcgtttacgt
    11761 atcttcactg aagatttcgg tcgagaatta ttccattatc ctgaaaaaac cccatttggt
    11821 cacgctattt ggagtggtcg tttacttaat cagacttgtt tcgtattcgg gtggcgatca
    11881 gaaaaagcag aattaaggct ttttcacttt gtagatgggc acttggtttc agaattagta
    11941 gatgcaaaag cagcttctag taatgtctta gcttttgaaa aagatggaaa agcttatctt
    12001 ttctcagcca ataacggacg tggcgaagtt gctctttatc aattagtaaa ataaatttaa
    12061 gaaggagaca atatggacaa gaaaaaaatc ttagtaacgg gtattgtgcc taaagaaggt
    12121 ctaagaaagc ttatggaccg atttgatgtt acttattcag aagatcgccc attttcacgt
    12181 gactatgtgt tagagcattt atctgaatat gacggatggt tactcatggg acaaaaaggt
    12241 gataaagaga tgattgatgc aggtgaaaac ttacaaatta tttctttaaa tgcggttggt
    12301 tttgatcatg tagatactgc gtatgctaaa gaaaaaggaa ttgtagtctc taattcccct
    12361 caagcagtac gagttccgac agcggaaatg acttttgctt taattttagc tgccagtaag
    12421 cgccttgcat tttatgatag tattgtaaga agtggagagt ggattgatcc gagcgaacaa
    12481 cgttatcaag gtttgacttt gcaaggttca acattaggta tttacggaat gggacgcatc
    12541 ggtttgacag tcgcaaattt tgctaaagca tttgggatga ctgttgttta caatgatgtt
    12601 tatcgacttc ctgaagataa ggaaaaagag cttggtgtaa cctatctcga atttgatcag
    12661 cttattgaaa cagctgatgt cataaccatc cacgccccag cattaccatc aacaatccac
    12721 aaattcaata aagatgtttt tgctaagatg aaaaatcgaa gctacttaat caacgctgct
    12781 agaggcccaa tcgtttctga agaagcactt attgaagctc tgaaagaagg tgaaatcgcg
    12841 ggtgcgggat tagatgtttt tgagaatgaa ccacaggtat ctgaaggctt gagaagtctt
    12901 gataatgtta tcatgagtcc acatgctggg acaggtacta ttgagggacg tagaactttg
    12961 gctgaagaag cagctgacaa catcattgcc ttctttgatg gcaagccaca aaatattgtc
    13021 aataagtaat tggctagtga gtgcagagcc atcttttgaa aaaatgggac ctcccatttt
    13081 tttacgggta aatgtaagcg cttaataatt taataaaggt gaacttatgc tagaagcttt
    13141 acataaattc tttgaagttt ttggtgcagc aactgttgta cccgctatga tttttattgt
    13201 cagccttttt ctaaaggtca atccaaggcg agctttcttt agcgccttac gagctggtgt
    13261 tggactcact ggctttggtt ggattatatc agcttttgct cctatggtga ctaagtatat
    13321 ccagcagatg gttaaaacta ctggtctcca tttaccaatt gtagatattg gttggcaggc
    13381 tggttctctc accgcctttt cttctgagat tggcctctct ttctttgttt ttggcttgct
    13441 aattgaattg gggcttttct tgcttgggat tacccgagta tttgtcccat ctaatctttg
    13501 gaataacttt ggctatatga tttggggtac aatggcctat gcagcaacag gtaattttat
    13561 attgtctttc gcatttatgg tttttgtctt actctattca ttagtgatgt cagaagtatt
    13621 ggcagatcga tggtcggagt attacggtgt taaaaatgca acgatcaact cgattcataa
    13681 tattgaaacc cttattccag ctcttattct tgatcctctt tggaacctac ttggtgtcaa
    13741 taaggtaaag ttgaatccag aatcattaaa gactaagtta gggatatttg gtgagccaat
    13801 gacacttggt tttatacttg gtgtgattat tggtgtatta ggtagtttac gtaaccttgc
    13861 aagtattgat acttggggag gtatacttgg ttttgcagtt gctcttgcag cggttatgac
    13921 tatttttcct ttgattacgg gggtatttgc tagtgctttt gctcctcttg ctgaagcagt
    13981 tgaacgcaat aagaaaaaag aaagtcaagc tgagcaagga gcactggata aaaaacgttg
    14041 gtttattgca gtagatgacg gtgttggttt tggagaacca gcaactatta tcgcaggtct
    14101 cattttagta ccaataatgg ttgttatttc cttgatttta cctggaaatg aagctttacc
    14161 agtcgttgac cttattgcta ttccttttat gattgaagct atgatagctg tgtcaaaagg
    14221 aaatatcctt aaagctattt tgaatggaat catatggttt tcactaggtc tttatgctgc
    14281 tagtgcccta ggtccaatct acacagaagc tgttaagcat tttggaactg ctttaccagc
    14341 aggggtaact ttgattatga gctttaatct cctagctcat cctttgacag ctctagtctt
    14401 ctttgcttgg atatctggga atccactcta tattggtgtg acagttatta tctatcttgc
    14461 tttcttgttc ttgcttcgta ctcgtcgtga atctatttac gcatacctta aaactatggc
    14521 tgataagaat agcggatatg aaggtagttg tgaaaaaatc tttattgctg aagattaaaa
    14581 atctgttgga ggaatactca tgaaaaaaat tgatggacat gctcatattg ttgatacaat
    14641 tgcaggattt aatggtaaag ggcgtcttaa tgcccttgga aacggttatg ctatttggga
    14701 tgatggtgat ttaattaagc tcatcccaga cgaatacggt gacaaagcat ttacagctga
    14761 atcttttttg aagtatatgg atgctaatgg tgtagataaa gcagtcattt tgcaaggtca
    14821 tcttaatggt tatcaaaatt attatacgca tttagctatt aaacgttatc cagaacgttt
    14881 tacaggcgca ttctctgttg atccatttgc agataatgca atgcaaattg taaaacgtca
    14941 tgttgaagtc cttggttttc gagctattaa atttgaaatt agtcaaggag ggggtatcca
    15001 tggataccgt ggacaaaaaa cgcctttccg tcttgataca gacccacatg tgagtcgtat
    15061 tttgacttat ctgttagact atccaggatt tgtagttaca gttgattatg gcaattggga
    15121 tcaaattagt caccaacctg atgctattgc taatttagct cgcctttata cgagtctcga
    15181 ttttgttgtt tgtcacctct cttttccaca tgttgagcat tcaaatcgat tgcgtgctga
    15241 acttaatatg tggaaaaact tcaacaatat ctatactgat atttcagcta ttcaagatat
    15301 tgatgcccca gatagttttc cttttccaaa atcagaacaa aatgtacgta ttgctaaaga
    15361 agttcttggg gctaagcgta tcatttgggg aactgattct ccatggtcag cgaccttcaa
    15421 tacttacgaa gaattagcta cttggctaga gaatgttgat attttcagtc aagaggaact
    15481 agaagatgtt atgtacaata atgctgagcg tgtttacttt aaagaagaac atgtaaaggc
    15541 taacagagca ggtatagatg atacaaccaa agagctcaat ctttattaga agagttttca
    15601 aggtacaaga gtttcttgta cctccttttt aaatcaacaa tgaggagaca aattatgact
    15661 tactacctta gcattgacta cggcggtaca aataccaagg cgcttatttt tgacaaatta
    15721 ggacaccaaa tcgctgtttc gagttttgaa actttaaaaa atgaggctca atctggtcat
    15781 cgtcaagtaa accttgttaa aacgtggaat gctataactt ctgctattag agaggttatt
    15841 caaatctcaa aactcagccc tgagcagatt agtgcagtag catgtattgg acatgggaaa
    15901 ggtctttatc tgctagataa taagttggag ccacttgaac aaggaatttt gtctacagat
    15961 aatcgtgcca aagatttggc gcaatatttc gaatctaaac ttgataatat ttgggagttg
    16021 actcggcaac atattttccc ttcacaaagt ccagttattt tacgttggct taaagattat
    16081 cagcccgaaa cctataaatc aataggggcg gtcctttctg caaaggactt tattcggtat
    16141 aagcttacag gaaaagtaca gcaagaatat ggtgacgctt caggtaatca ttggataaat
    16201 tttcaaacag gaacttatga tccagctatt ttagattttt ttggcattag agagatagaa
    16261 aactcacttc ctgaacttat agatagtgca gatttagttc ctgggggaat tagttctcaa
    16321 gcagcaaaag agactggtct tgtagaagga acccccgttg ttggagggct ctttgacatc
    16381 gatgcttgtg ctcttggatc aggtgtttta gagtcagata cttttagtgt tatttcggga
    16441 acttggaata ttaatactta tccaagttta aaaccagcaa agcaagatag tggtcttatg
    16501 acttcctatt ttccagatcg tcgttatctc ttagaggcaa gtagccctac ttctgcaggg
    16561 aatcttgatt ttatgttaaa aatgctcatg catcaagaaa ttgataacgc taaatctagt
    16621 ggaggttcta tctatgataa tttagaagaa tttctcactc atactgatgc cacacatcat
    16681 ggacttattt tctttccgtt tctttacggt agtaacacat cacaagatgc tagcgcttgc
    16741 ttttttgggc taacaactaa atcgacgaaa tctcagatga tacgtgcggt atatgaaggt
    16801 attgcgtttg cacataagca gcatatcact gatttaataa aaagtagggg cggtgtgcca
    16861 aaaataattc gtttctctgg cggagctacc aactcaccag catggatgca aatgttttct
    16921 gatatcttaa accttcctat tgaaacagta gaaggcacag aattaggagg attaggagga
    16981 gctattttag cacgtcatgc tttagataag atttcgttaa aggaagcagt ccaagatatg
    17041 gttcgtgtaa aagctattta taaacctcaa ttatccgaag taaaggggta cgaaaaaaaa
    17101 tatcacgcct accaaaaatt attggaaaca ctggatccta tttggtcgga actcggtcat
    17161 ctgaataagt agggatatca aaggagttgt tatgaaacaa gaagatatta ttatcaatag
    17221 tattgtattc aaaaaacagt tagacaaagg ttttacacaa gttcagctca ttaagttagt
    17281 aaaggattta ggattttcac gatttgagat acgtcaagaa ttgcttcaag atccagatag
    17341 agagctaccc gctttaaaag cagaggctga cttttatgat atcaatcttt actatagtgc
    17401 taatgaagac cttatcaaag gagggaaagt taatccttat cttaataaag gccttaaaga
    17461 agcatcacag ttaggagctc ctttcattaa gctaaatgtt ggacaaacca gaaacttaag
    17521 taaagaagag ttagaacctc ttaaagagat attgaaaagc caaactattg gtataaaggt
    17581 ggaaaataat caagacccta aagcagctac agtggagaat tgccaatatt ttatgactct
    17641 agtgaaagaa ttacaaatac caataagttt tgtttttgat actgctaatt gggcctttat
    17701 caaccaagac ctataccaag cggtaaataa cttagcttgt gataccacct accttcactg
    17761 taaaaatttt atacaggtag ctggaaagcc ccacctctcg aagagcctct tcgaaggaga
    17821 gattaatctg actgatttgt taaagagttt ctctaattgt gagtatctag ctctagaata
    17881 ccccacagag ttggagatat taaaaaggga tgttcaacgt ttaatctcta tcagtaatag
    17941 tcaaaacttt taatgaaaag gctttaaaaa gatgttataa taagtctgta tgaattcgag
    18001 tagaaaagag gtatattctt gattattctc gataaaaaaa gttatgacct cctcttttac
    18061 ctattgaaat tagaggaacc tgaaacagtt atggcaattg ccaacgcact taatcagtct
    18121 agacgtaaag tgtattatca cttagagaag ataaacgatg cactgcctag cgatgtgcct
    18181 cagattgtta gttatccacg tgtaggaatc ttgctaacag aaaaacaaaa agcagcctgt
    18241 cgtcttttat tagatgaagt gactgattat agttacgtca tgaaaagtag tgagaggttg
    18301 cagctgtctt tagtatctat cgtagtagct aaggaccgca taacgattga taggttgatg
    18361 caactaaacg atgtttctcg aaatactatc ttaaacgatt taaacgaatt aagaagtgag
    18421 ttagcagaga aagaatataa tttacagtta caatcaacaa aatgtcgtgg ttatttttta
    18481 gatggtcatc cattgtccat tatccagtac ttatataagc tcttagatga tatctaccat
    18541 aatggaagta gtagttttat agaccttttt aatcataaac tgtctcaagc ttttggtgcc
    18601 agcacttatt tttctaaaga ggttcttgat tattttcatc attatctctt catttctcaa
    18661 cgaagtctag gtaagaaaat caacagtcaa gatggtcagt ttatgattca gattttgcct
    18721 tttatactaa tggcttatcg taagatgcga ttaagtcctg aagtacagac ctctcttaat
    18781 agtgatttta gcttggtttg gcaacgtaag gaatatgaga ttgctaaaga gttggctgat
    18841 gagctggaag aaaattttca gttatcactg gatgagattg aagtgggact agtagccatg
    18901 cttatgctta gtttccgcaa ggaccgtgac aatcatttag agagccagga ttatgatgat
    18961 atgcgagcta ctctaaccag ttttttgaaa gaattggaag aacgatatca ccttcacttt
    19021 gttcataaaa aggacttact aagacaactt cttactcact gcaaggcact cttatatcgt
    19081 aaacgttatg gtattttttc tgttaatcct ttaacagagc atattaaaga caaatatgaa
    19141 gaactttttg ccataacctc gtcttctgta aagcttttag agaaagcttg gcaaatcaaa
    19201 ttgaccgatg atgatgtagc atatctaacg attcatttag gaggggaact tcgtaatagt
    19261 caacaatctc ctaataaact taagttagtt attgtatctg atgaaggaat agcgattcag
    19321 aaacttcttt taaagcaatg tcaacgctac ttaacaaata gtgatataga agctgttttt
    19381 acaaccgaac agtatcaaag tgtgagtgat cttatgcatg tagatatggt tgtctctact
    19441 agtgacgctt tagaatctcg ttttccgatg ttagtagttc accctgtttt gacagatgat
    19501 gatattattc gcttgattcg cttttctaaa aaaggtaact gtgcaaatag taatcaattt
    19561 accaatgaac ttgaaaaaac aattgctcaa tatgtcaagg aagatagtga acgctacgtg
    19621 ctgaaatcta agattgagaa acttattcat caagaattgc tccaagacgt ccttcccctt
    19681 caaagtacag tttgttaaaa tataatttta tggtagcctc tcagcttttc tgaagacaga
    19741 aaaagaagct ggggcttttt tggtgctttt aaacaatgtg ctagatattt aatcgcaatg
    19801 agttcctcta tgacatgata attaaagttg agaaagctag tacttttaat gggagtactt
    19861 tttatttgtg cttattttaa ttaaaattat gagtctaatt tggaacagac agcccagttt
    19921 tgaacacata aatgtgtgac acttggtctt tttttcaaaa gtatttgaac ttatactagt
    19981 agatgtaaga aacaattaat caggaggaaa taaaatgcct aacgttaaag atattactag
    20041 agaatcatgg attttatcaa cattcccaga atggggtaca tggctaaacg aagaaattga
    20101 agaagaagtt gttgctgagg gcaactttgc aatgtggtgg ttaggaaact gtggtgtttg
    20161 gattaaaaca ccaggtggtg ctaatgttgt tatggaccta tggtcaaacc gcggtaaatc
    20221 aacaaaaaaa gtaaaagata tggttcgtgg acaccaaatg gctaatatgg caggtgttcg
    20281 taaactccaa ccaaacttac gcgcacaacc aatggttatt gatccatttg ctattaatga
    20341 attggattac tatcttgttt cacatttcca cagtgaccac attgatatca acactgcggc
    20401 agccattatt aataatccta acttagacca tgttaaattt gttgggccat atgaatgtgg
    20461 tgaaatttgg aaaaaatggg gagttccaga agaacgaatc atcgtgatta aaccaggtga
    20521 atcatttgag tttaaagata tcaaagttac tgctgttgaa tcatttgacc gtacttgctt
    20581 ggtgacactt ccagtagatg gtgcagaaga acatgatgga gaacttgctg gtttagccgt
    20641 gactgatgaa gaaatggctc gtaaagcagt taactatatt tttgaaacac caggtggaac
    20701 aatctatcat ggagctgact cacatttttc aaattatttt gctaaacatg gtaaagacta
    20761 caagattgac gtagctatta acaactatgg tgataaccca gttggtattc aggataaaat
    20821 gacttcaatc gatcttcttc gtatggcaga aaatctccgt gccaaagtca ttattcctgt
    20881 acactacgat atctggtcta acttcatggc ttctacagac gaaatcttac aattgtggaa
    20941 aatgcgtaaa gaacgccttc aatatgactt ccatccattt atttgggaag ttggaggtaa
    21001 atacacttat ccacaagata aagatagaat tgaataccat cacccacgtg gttttgatga
    21061 ttgcttcgaa caagaatcaa atattcaatt taaagcactt ctataatggc taatgagaga
    21121 ctgtatgtcg catgatatgc agtttttcaa ttttaaaata tgttactaaa atcacaatta
    21181 agattaggag aaataatgtc agaatttgat acaaaatcat atttagaaaa acttgatgca
    21241 tggtggagag cagctaatta tatttctgca gcacaaatgt atctaaagga taaccctctc
    21301 ttgagacgag agctggttga aaacgactta aaggttcatc caattggtca ctggggcact
    21361 gtacctggac aaaattttat ctatgctcac ttaaatcgcg ctattaataa atacgattta
    21421 gacatgttct atattgaagg tccaggtcat ggtggtcaag ttatggtatc taattcatat
    21481 ttagatggtt catatactga attaaaccca aatattgagc aaacagagga tggctttaag
    21541 cagttatgta aaatcttctc tttcccaggt ggaattgcat cccatgctgc accagaaacg
    21601 ccagggtcaa ttcatgaagg cggtgaactt ggttacgcac tttctcatgc gacaggtgcc
    21661 atcctagaca atccagatgt tattgctgcg actgttattg gtgatggtga aggtgaaaca
    21721 ggaccactta tggctggttg gctatctaat acctttatca atccagtaaa tgatggtgct
    21781 gttctaccaa tcttctattt aaatggtggt aaaatccata acccaactat ctttgaacgt
    21841 aagacagatg aagaattatc tcagtttttt gaaggattag gttggaaacc tatttttgca
    21901 gatgttgttg aactctctga ggatcatgcg gctgctcatg ctttgtttgc agaaaaatta
    21961 gatcaagcta ttcaagagat taaaaccatt caatcagaag cacgacaaaa accagcagaa
    22021 gaagctatcc aagcaaaatt ccctgtctta gttgcacgta ttcctaaggg ttggactggt
    22081 ccaaaagctt gggaaggaac accaattgaa ggtggcttcc gcgctcacca agtaccaatt
    22141 ccagtagatg cccaccatat ggaacatgtt gattctcttt tgtcatggct tcaatcatac
    22201 cgtccagaag aattatttga tgaaaatggt aaaatcgttg atgagattgc tgctatttca
    22261 ccaaaaggtg atcgtcgcat gtccatgaat ccaataacca atgctggtat tgttaaagca
    22321 atggatacag cagattggaa gaaatttgct cttgatatta atgttccagg tcaaattatg
    22381 gcacaagata tgattgaatt tggaaaatat gcagcagatt tggtagatgc taatccagat
    22441 aatttccgta tttttggtcc agatgaaacg aaatcaaatc gtcttcaaga agtgtttaca
    22501 cgtactagcc gtcaatggct tggtcgtcgt aaaccagatt atgacgaagc tttaagtcca
    22561 gctggacgtg ttattgattc ccaattgtct gaacaccaag cagaaggttt cttagaaggt
    22621 tatgttttaa ctggtcgtca cggcttcttt gcttcatacg aatcattcct tcgcgttgta
    22681 gattcaatgg taacacaaca tttcaaatgg ttacgtaaat caaaaacaca cacaacatgg
    22741 cgtaaaaatt atccagcgct taacctaatt gcagcttcaa ctgttttcca acaagatcat
    22801 aatggttata ctcaccaaga tccaggtatt ttaactcatt tagctgaaaa aacacctgaa
    22861 tacattcgtg aatatttacc agcagatact aactcacttc tagctgttat ggataaagcg
    22921 tttaaagctg aagataaaat taatttaatt gtgacatcta agcaccctcg tccacaattt
    22981 tactctattg ctgaagcaga agagttagtg gcagaaggat ataaggtgat tgactgggct
    23041 tcaaatgtgt cgcttaatca agagccagat gttgtctttg ctgcggcggg aacagaacct
    23101 aacttagaag ctttggcagc tattagcatt cttcataagg ctttcccaga acttaagatt
    23161 agatttgtta atgtattaga catcctaaaa cttcgtcatc cttcacaaga tgctcgcggt
    23221 ttatcagatg aagagtttaa caaagtcttt accacagata aacctgttat ttttgctttc
    23281 catggttatg aagatatgat tcgagatatt ttctttagtc gtcacaatca taatttgcat
    23341 acgcatggtt accgtgaaaa tggtgacatc acaacaccat ttgatatgcg cgtgatgtca
    23401 gaattggacc gattccatct ggcacaggat gcagctcttg cttctttagg aaataaagca
    23461 caagccttca gtgatgaaat gaatcaaatg gttgcttatc acaaagacta tattcgtgaa
    23521 cacggagacg atattccaga agtacaaaat tggaaatggg aaaatatcaa gtagtcactt
    23581 ttttcaggcc tatacttaca aacaaaaaca cttttagatt ttttctaagg gtgttttgtt
    23641 attttataga agaatgaccg ctttttcaaa agtattaagt ggtcactttt aaagttgtaa
    23701 tattaatact aataagtctt taaataattc aataatgcca taacaataat ggtgtgggct
    23761 aattaaaata tctctaagca aaggtgttga cataaagaga ccatacatga tattatagct
    23821 atgttctgtt tcggcagttt atttttttga gtaaaaaagt gactaccctt ttacgataac
    23881 ttaacgtcac tttatagata agacacataa ccttaaaaat aagatacgaa atttttagta
    23941 ggagataaga atgacaaata ttttagaagt gaagaattta accaaaattt ttgggaaaaa
    24001 gcaaaaagct gctcttgaaa tggttaagca aggaaaaagt aagacagaaa ttcttgaaaa
    24061 aacaggagcg actgtaggtg tctatgatgc gagttttgaa ataaaagaag gcgaaatttt
    24121 cgttatcatg ggactttctg gttcaggaaa atcaacttta gttcggatgt taaaccgttt
    24181 gattgaccca tcatcaggaa atatctattt agatggtaaa gatattgcaa agatgaatgt
    24241 agaagattta cgtaacattc gtcgtcatga tatcaacatg gttttccaaa attttggtct
    24301 tttcccacat cgtacaattt tagaaaatac agagtttggg ttagagatgc gtggtgtttc
    24361 taaggaagaa cgtacaacgt tagctgaaaa agctttggac aatgcaggtc tattaccgtt
    24421 taaagatcag taccccagtc aattatcagg aggaatgcaa cagcgtgttg gtttagcacg
    24481 tgcccttgct aacagcccta aaatccttct gatggatgag gccttctcag ctttagaccc
    24541 attaattcgt cgtgaaatgc aagatgagct tcttgatctt caagacacga ataaacaaac
    24601 tattattttt atcagccatg atttgaatga agcgttacgt attggtgatc gtattgctct
    24661 gatgaaagat ggagaaatca tgcagattgg tacaggagaa gagattttga ctaatccggc
    24721 taatgatttt gttcgtgagt ttgttgaaga cgttgatcgc tcaaaagtgt taactgctca
    24781 aaatattatg ataaaacctt taacaactgt tttagaaatt gatggcccac aagttgcttt
    24841 aacaagaatg catcgtgagg aagtctcaat gttaatggca acgaatcgtc gtcgccaact
    24901 tctgggatcc ctaactgcag atgctgcaat tgaagctcgt aagaaaggtt tgccgctttc
    24961 tgaagtgatt gataaagatg ttgtaacggt ctcaaaagat acagttatta cagatatcat
    25021 gcctttaatt tatgattcat ctgctccgat agccgttact gatgataatg atagactcct
    25081 aggggttatc attagaggac gtgtgatcga agcacttgct aacgttcaag atgaaacagt
    25141 agtagaatcg ccaaaagaaa cggtagaagc ttaatagaga ggacaattca ttttggaaaa
    25201 tttattacaa cataagttac cggtcgcacc atttgtagag tcaacgacca attggattac
    25261 gaaaactttt tctggtttat ttgactttat ccagacaatc ggtaatgcat tgatggattg
    25321 gatgacaaaa acattactat tcattaatcc tttacttttt attgtactaa ttacaattgc
    25381 tgtatttttc ttagctaaga aaaaatggca attaccgaca tttactttca ttggtttgct
    25441 atttatctat aaccaagggc tgtgggaaca gttgattaat acttttaact tggttttagt
    25501 agcaagttta atttctatta ttattggtgt accactaggt atttggatgg caaaaagtga
    25561 taaagttaaa caagttgtta atccaattct tgactttatg caaactatgc cggcattcgt
    25621 ttaccttatt cctgcagtag ccttctttgg tatcggtatg gtaccaggtg tttttgcctc
    25681 tgttgtattt gctctgcctc caacagtgcg ctttactaac ttggctattc gtgaaatccc
    25741 acttgaatta atagaagcat ctgattcatt tggttcaaca gctaagcaaa aattattcaa
    25801 agttgaacta ccacttgcta agaacactat tatggcagga atcaatcaaa ctatgatgtt
    25861 ggctctttct atggtggtta caggatctat gatcggtgca cctggacttg gtcgtgaggt
    25921 gttatcggcg cttcaacatg ctgatattgg tacaggattt gttagtggtc tatcgctagt
    25981 tattttagca atcgtgttag accgtgttag tcaattcttt aattccaaac cgggtgagaa
    26041 gcaagctaaa acatcaaaag ttaaaaaatg ggtaggttta ggggccctgg ctctcttcat
    26101 tctagcagct cttggtcgca ttgttgtcaa tatgacttca ggaaatgaag ccaaaggtca
    26161 aaaagtgaag atagcttatg tccaatggga ttcagaagtt gcttcaacca acgttattgc
    26221 tgaagtttta aaatctaaag gatatgatgt tgaattgaca ccacttgaca atgcggtcat
    26281 gtggcaaaca gtcgccaatg ggaatgctga ttttacaaca agtgcttggt taccaaagac
    26341 ccacgggcaa tattttaata aatacaaaaa tagtttagat gacttaggtc ctcacgttga
    26401 gaacgtcaaa ataggtttag ttgttcctaa atacatgaac gtaaatagta ttgaagaatt
    26461 atcaaatcaa gcagataaac aaattacagg tatcgaacca ggtgcaggta ttatgaagtc
    26521 tgctaaacaa tcccttaaag attatccaaa cctttcaagt tggaagttgt tatcagcatc
    26581 aacaggagct atgacaacaa ccttagggaa ggctatcaaa aacaaagatc aagtagtcat
    26641 tacaggttgg tcacctcact ggatgtttgc taagtatgat ttaaaatacc ttaaagatcc
    26701 taagaaatca tttggtggtg aagaacatat caatacaata gcacgcaaga atttgaaaaa
    26761 agatatgcca aaagtatata aaattatcga taagttcaag tggactaaag aagatatgga
    26821 atctatcatg ttagatatgg ataaaggtat ggagcctgct aaagcagctc aaaaatggat
    26881 taaaaaccac aaaaaagaag tttcagagtg gactaaatag tatagaaaaa aacacgtctc
    26941 tcttagagcg tgttttttaa tgtgacttaa gtcgttttag ctatttaata taagttttat
    27001 attattttaa tacatcaaaa gtgagagaat gaaagaaatg gcaggttata aagtattac
//
LOCUS       DATROY010000010        26523 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.010, whole genome shotgun sequence.
ACCESSION   DATROY010000010 DATROY010000000
VERSION     DATROY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26523
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(298..684)
                     /gene="rplQ"
                     /locus_tag="VBD11_000411"
     CDS             complement(298..684)
                     /gene="rplQ"
                     /locus_tag="VBD11_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6953388.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(699..1637)
                     /locus_tag="VBD11_000412"
     CDS             complement(699..1637)
                     /locus_tag="VBD11_000412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6953389.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1687..2070)
                     /gene="rpsK"
                     /locus_tag="VBD11_000413"
     CDS             complement(1687..2070)
                     /gene="rpsK"
                     /locus_tag="VBD11_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6953390.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2088..2453)
                     /gene="rpsM"
                     /locus_tag="VBD11_000414"
     CDS             complement(2088..2453)
                     /gene="rpsM"
                     /locus_tag="VBD11_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6953391.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2471..2587)
                     /gene="rpmJ"
                     /locus_tag="VBD11_000415"
     CDS             complement(2471..2587)
                     /gene="rpmJ"
                     /locus_tag="VBD11_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6953392.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2613..2831)
                     /gene="infA"
                     /locus_tag="VBD11_000416"
     CDS             complement(2613..2831)
                     /gene="infA"
                     /locus_tag="VBD11_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6953393.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2947..3585)
                     /locus_tag="VBD11_000417"
     CDS             complement(2947..3585)
                     /locus_tag="VBD11_000417"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6953394.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3680..4984)
                     /gene="secY"
                     /locus_tag="VBD11_000418"
     CDS             complement(3680..4984)
                     /gene="secY"
                     /locus_tag="VBD11_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6953395.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5005..5445)
                     /gene="rplO"
                     /locus_tag="VBD11_000419"
     CDS             complement(5005..5445)
                     /gene="rplO"
                     /locus_tag="VBD11_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6953396.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5570..5749)
                     /gene="rpmD"
                     /locus_tag="VBD11_000420"
     CDS             complement(5570..5749)
                     /gene="rpmD"
                     /locus_tag="VBD11_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6953397.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5764..6258)
                     /gene="rpsE"
                     /locus_tag="VBD11_000421"
     CDS             complement(5764..6258)
                     /gene="rpsE"
                     /locus_tag="VBD11_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6953398.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6277..6633)
                     /gene="rplR"
                     /locus_tag="VBD11_000422"
     CDS             complement(6277..6633)
                     /gene="rplR"
                     /locus_tag="VBD11_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6953399.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6734..7270)
                     /gene="rplF"
                     /locus_tag="VBD11_000423"
     CDS             complement(6734..7270)
                     /gene="rplF"
                     /locus_tag="VBD11_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6953400.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7380..7778)
                     /gene="rpsH"
                     /locus_tag="VBD11_000424"
     CDS             complement(7380..7778)
                     /gene="rpsH"
                     /locus_tag="VBD11_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6953401.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7933..8118)
                     /locus_tag="VBD11_000425"
     CDS             complement(7933..8118)
                     /locus_tag="VBD11_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6953402.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8136..8678)
                     /gene="rplE"
                     /locus_tag="VBD11_000426"
     CDS             complement(8136..8678)
                     /gene="rplE"
                     /locus_tag="VBD11_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6953403.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8702..9007)
                     /gene="rplX"
                     /locus_tag="VBD11_000427"
     CDS             complement(8702..9007)
                     /gene="rplX"
                     /locus_tag="VBD11_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6953404.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9087..9455)
                     /gene="rplN"
                     /locus_tag="VBD11_000428"
     CDS             complement(9087..9455)
                     /gene="rplN"
                     /locus_tag="VBD11_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6953405.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9480..9740)
                     /gene="rpsQ"
                     /locus_tag="VBD11_000429"
     CDS             complement(9480..9740)
                     /gene="rpsQ"
                     /locus_tag="VBD11_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6953406.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9766..9972)
                     /locus_tag="VBD11_000430"
     CDS             complement(9766..9972)
                     /locus_tag="VBD11_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6953407.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9982..10395)
                     /gene="rplP"
                     /locus_tag="VBD11_000431"
     CDS             complement(9982..10395)
                     /gene="rplP"
                     /locus_tag="VBD11_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6953408.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10399..11052)
                     /gene="rpsC"
                     /locus_tag="VBD11_000432"
     CDS             complement(10399..11052)
                     /gene="rpsC"
                     /locus_tag="VBD11_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6953409.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11065..11409)
                     /gene="rplV"
                     /locus_tag="VBD11_000433"
     CDS             complement(11065..11409)
                     /gene="rplV"
                     /locus_tag="VBD11_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6953410.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11425..11703)
                     /gene="rpsS"
                     /locus_tag="VBD11_000434"
     CDS             complement(11425..11703)
                     /gene="rpsS"
                     /locus_tag="VBD11_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6953411.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11802..12635)
                     /gene="rplB"
                     /locus_tag="VBD11_000435"
     CDS             complement(11802..12635)
                     /gene="rplB"
                     /locus_tag="VBD11_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6953412.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12653..12949)
                     /locus_tag="VBD11_000436"
     CDS             complement(12653..12949)
                     /locus_tag="VBD11_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6953413.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12949..13572)
                     /gene="rplD"
                     /locus_tag="VBD11_000437"
     CDS             complement(12949..13572)
                     /gene="rplD"
                     /locus_tag="VBD11_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6953414.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13596..14222)
                     /gene="rplC"
                     /locus_tag="VBD11_000438"
     CDS             complement(13596..14222)
                     /gene="rplC"
                     /locus_tag="VBD11_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6953415.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14295..14603)
                     /gene="rpsJ"
                     /locus_tag="VBD11_000439"
     CDS             complement(14295..14603)
                     /gene="rpsJ"
                     /locus_tag="VBD11_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6953416.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14810..16090)
                     /locus_tag="VBD11_000440"
     CDS             complement(14810..16090)
                     /locus_tag="VBD11_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6953417.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16195..17685)
                     /locus_tag="VBD11_000441"
     CDS             complement(16195..17685)
                     /locus_tag="VBD11_000441"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6953418.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17805..18821)
                     /gene="adhP"
                     /locus_tag="VBD11_000442"
     CDS             complement(17805..18821)
                     /gene="adhP"
                     /locus_tag="VBD11_000442"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6953419.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19000..21642)
                     /gene="adhE"
                     /locus_tag="VBD11_000443"
                     /gene_synonym="adhC"
     CDS             complement(19000..21642)
                     /gene="adhE"
                     /locus_tag="VBD11_000443"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6953420.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21911..23689)
                     /locus_tag="VBD11_000444"
     CDS             complement(21911..23689)
                     /locus_tag="VBD11_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6953421.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23686..24066)
                     /locus_tag="VBD11_000445"
     CDS             complement(23686..24066)
                     /locus_tag="VBD11_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953422.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24073..24510)
                     /locus_tag="VBD11_000446"
     CDS             complement(24073..24510)
                     /locus_tag="VBD11_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6953423.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24662..25660)
                     /gene="ruvB"
                     /locus_tag="VBD11_000447"
     CDS             complement(24662..25660)
                     /gene="ruvB"
                     /locus_tag="VBD11_000447"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6953424.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(25957..>26523)
                     /locus_tag="VBD11_000448"
     CDS             complement(25957..>26523)
                     /locus_tag="VBD11_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6953425.1"
                     /translation="DECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLN
                     DLIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVF
                     VGLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQ
                     SIMFSKMIDDKHLIKKLELEWQEDITGH"
BASE COUNT         8021 a         5477 c         4496 g         8529 t
ORIGIN      
        1 tttagtatag acataactat tcctctatat tcaattaaga gaaattatat aaccactatt
       61 gagaaatgta gtctagctat taaactcttt gctcatcgaa aatatccaat aaatataata
      121 cataaaacgc ctgcttacga aatataaaca aaattgtttg catttcgtaa acaagcgtta
      181 cctatttaac aatatatgat gagtgttccc gctgagaata attctcagcg gtagaccaga
      241 gctagactaa gaatcgattg attccatcat cataacactc aacaaaattg ataaaaatta
      301 tactaattca ataattgcca ttggggcagc atcgccacgg cgtggttctg ttttaagaat
      361 acgagtgtat cctccgttac gttcagcata acgaggtgcg atgtcgtcaa aaagtttttg
      421 aagagctgtt gttgatgtat atttatcaga agcttcatca tagttttctg atgcaatttc
      481 attacgtaca taagcagcag cttgacgacg agcatgtaaa tcaccacgtt tacctagagt
      541 aatcattttt tcaactgttt tacggatttc tttagcacgt gcttcagtag ttacaattga
      601 ttcgttgata agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt gtgagctagt
      661 gcgtcctagt ttacggtaag ccattatgtc ctcctatttt atttatcgtt ttttaatcca
      721 agacctagat cggcaagttt gattttaact tcttcaagac tcttacgtcc taagtttcgg
      781 actttcatca tttcaggctc agttttttct gttaaatcaa atactgtatt aattccagca
      841 cgttttaaac agttatatga gcgcactgac aaatcaagtt cctcaattgt gcggtcaagc
      901 actttctcat cgttcacttt ctctgtttcc ttcattactt cagttgcttt agcaacctct
      961 gttaaatcag taaacaagtt taagtgttca attaagacgc gagctgaaag accaagagca
     1021 tcctcaggaa tgattgtgcc atttgtcatg atttcaatag ttaatttatc aaagccatca
     1081 ttactaccta cacgagcagg ttcaacttga taattaactt ttttcactgg tgtatagatt
     1141 gaatctacag ccaatgttcc tactggtgca tcatcttttt tatttccttc tgctggaaca
     1201 taacctcggt ttttagcaac ggtcattgtt gctttcaaac tgtgaccttc agcaattgtg
     1261 aaaagataat ggtcagggtt tacaatttcg atatcactat ctgttaaaat atctccggca
     1321 gtaatttctg caggaccttc aacgtctagt tcgataatct tttcgtcttc gacgtaagat
     1381 ttcacagcaa gtccttttac attaaggatg atttgcatca catcttcacg tacacctggg
     1441 atagtatcaa attcgtgtaa tactccatca attttgattg atgtgactgc tgcacctgga
     1501 agtgaagaca gaagcacacg acgaagagag tttcctagag ttgtaccgta gccacgttct
     1561 agtggttcga tgacaaatct gccgtaatct ttattttcat caatttttgt tattattggt
     1621 ttttcaaact caatcattta gttactccct cttaaacgaa aagttgtgta ctataaaatg
     1681 ctatgattat acacgacgac gttttggagg acgagcacca ttatgtggta caggagtcac
     1741 atcacgaatt gcagttactt caagaccagc tgcagcaagg gcacggatag cagattcacg
     1801 acctgaacca ggacctttta cagtaacttc aacagtttta agaccgtgtt cttgtgcaga
     1861 tttagcagca gcttcagcag ccatttgagc agcaaatgga gtagatttac gagaaccttt
     1921 aaatccaaga gcaccagctg atgaccatgc aagagcgtta ccatgcacat ctgtaatcat
     1981 aacaatagtg ttattgaatg tagcgtgaat atgagcaaca ccagattcga tgttcttttt
     2041 cacacgacgt ttgcgtgttg gtttagccaa ttgttttacc tcctatttta ttttttctta
     2101 cctgcaatcg caacagcttt acctttacga gtgcgagcgt tatttttagt attttgtcca
     2161 cggacaggaa gtccacgacg gtgacggatt ccacggtatg aaccgatttc catcaaacgt
     2221 ttaatattca agtttacctc acgacgtagg tcaccttcaa ctttgattgc atcaacttca
     2281 cgacggatag cgtcctcttg atctggtgtc aaatctttaa cgcggatatc ttcagaaata
     2341 cctgcagctg ctaagatttt tttagatgta gaaagaccaa ttccgtatac ataagtaagt
     2401 gaaattacta cacgtttgtc atttggaata tcaactccag caatacgagc cattttatct
     2461 cctttctata ttatccttga cgttgtttgt gttttggatt tgtcggacaa attaccataa
     2521 cacgaccgtt acgacgaata actttacagt attcgcaaat tggtttaacc gatggtctta
     2581 ccttcatgtt ttaatccctc caattatttc gactatttaa agcggtatgt gatgcgtcca
     2641 cgtgttaaat cgtatgggct catttcaaca gtaacacgat cacctaccaa aatacggatg
     2701 tagtttttac ggatttttcc tgatacagtt gccaaaattt ggtgtccatt ttccaactca
     2761 acagtaaaca ttgcgttagg catagtttca acaactttac cttcaatttc aatcacatct
     2821 tcttttgcca cgcaaaagta ccccctaaat ttcgatttga tgtcctctat aaagaggtaa
     2881 caattataag tcagactaac ttagtatatc attactaatc tacttatgca agtagattag
     2941 cataatttat ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt ctgttatttc
     3001 ttgattacct tcaatatctg taacaagacc aagcttacga tagtgttcaa gaataggttc
     3061 tccttgagca atattaacgt ccaagcgacg tttgacagtt tcaggcttat catcttcacg
     3121 ttgatagtaa tcttcttctt tataatctac tggtgggttg aacactttgt ggaaagtttc
     3181 accagtttta cgattgataa tacggccact caaacgctct ataaggcatg ttggatccac
     3241 tttaatatta ataacaccat ctaagcgtag tcctagttct tcaagcgtag catctaaggc
     3301 gtgtgcttgc tcaatagtac gtggatatcc atcaagtaaa aaaccttttt ctgcgatatc
     3361 atcctcagct aagcgctctt ttacaatccc gtttgttact tcatcaggaa ccaattcacc
     3421 tttatcaata taacttttag ctaaacgtcc catttgggtt tgattagcca ttgcggcgcg
     3481 gaacatatcc cctgttgaga tgtgagcaac accaaattct tcaacaatct tagctgcttg
     3541 agtaccttta ccagcaccag gcaaacccat aattaaaaga ttcattatag tctccttatt
     3601 ttgtttttaa ataaatccaa cacagcatgt cggacttatt aaaaaacaaa atagaaggtt
     3661 gacagagtca acctatattc tattctgttg tattcatgaa tccaacatag cgtctcttca
     3721 aaagatagcc ctcaagttgt ttcatacctt caatacctgt tgcaatcaaa atgagtaagc
     3781 ttgtaccacc taacgcaata cttgatgaca aatgcaacgc ttgttgagca ataattggta
     3841 acaaggaaat aaatgctaga aatactgaac caattgttgc taattttttc aatagcgatg
     3901 acatatactc ttctgtttct cgtcctggtc gaatacttgg aatgtaggac gaattttttt
     3961 gaagattctc tgctgtcttt tcagggttta cttgtacaaa agtatagaag aaagagaaca
     4021 gaataatcaa gatagcatag ataatcatac cgactggggt ttgataatta agtaattctt
     4081 ggagtttagt aagccaagga atttctttac cattttgaaa gaaaggaata atcgtacttg
     4141 gaattgttgt aatcgagcta gcaaagataa cgggaataac accagctgga tttactttta
     4201 gaggaagata tgaacttgta ggagctcctt gaacaagttt tgtatattgg attggaattt
     4261 tgtattcagc ttgttgaata aatgttgtaa aaaatacaat tgctaatact gctacaataa
     4321 gaatcccgac aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt acaaagaaat
     4381 cttcgtagat agttgtaatt gcacttggaa tggaagaaat gatccccgca aagataatca
     4441 tagacactcc atttccgaat cccttatcag taatttgttc acctagccaa gtaaccacca
     4501 tactacctgt tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc ggagttttaa
     4561 ctaaagcaac acttgataga gtgttaaaac cagcagtaat accaattgat tgtacgaatg
     4621 caaggaacaa agaaatataa cgtgtagctt ggttaagctt acgacgacct acctctcctt
     4681 gttttcccca ttcaacaaac ttcggaagaa tatccatttg aagtagctga acaacaatag
     4741 acgcagtaat gtatgggcta actcccatag agaataccga aaagtttctc attgcattac
     4801 cactaactaa gttaagcata tttagaaatg gaagttcacc catttgttct aaactcttaa
     4861 cattgatgcc cggtactgtg atatgcgttc cgatacgaaa tacaagaagg ataaagatag
     4921 taaataaaat tttatttctt accatcttta ctttgagtgc atctctaagt aattttaaaa
     4981 acataatgag ttacctctca ttaattagat gacttcgatt gaaccacctt tagcagtgat
     5041 agctgcttca gcagatttag aaaatttagc tgctttaaca gacaatttct tggttaattc
     5101 accattacca agaattttaa cgcctgattt ttcagcacgc acgattccag cttctttaag
     5161 aacaactgga gttacttcag taccatcttc aaagacattt aattggtcaa ggttcacaag
     5221 agcatattct tttgcattga tgtttgagaa cccacgtttt ggcatacgac ggaacaatgg
     5281 agtttgtcca ccttcaaaac caaggcgtac tccaccacca ctacgcgctt tttgtccttt
     5341 ttgaccacgt ccagaagttt taccattacc tgaagatgta ccgcgaccca cacgattgcg
     5401 gactttacgt gatccttctg caggttttaa ttcatgaagt ttcattattt tttctcctta
     5461 tttgtaaaat gctaacgcct ttacgagggg aaaggtactc cccgcaaaga ctcgcctata
     5521 cgatttgttt aaatcgcacc aataacaatc agtgcgattt agcttttaat taagcttctt
     5581 caacagttac taagtgagag atagcgttaa ccataccacg gatggcagca ttatcttcct
     5641 taacaactga agagtttaat ttaccaagtc caagggcaac aactgtttta cgttgttctg
     5701 gtttgcgacc gattggagac ttagtcaaag taattttaat ttgagccatt ataatcccct
     5761 ttcttatgct aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt ctgcacgttt
     5821 taattgtttc aagccctcaa cagttgcgcg aacaatgttg attggagtgt ttgaaccaag
     5881 tgatttagat gtaatatctg caacacctgc taattcgatg acggcacgaa cagcaccacc
     5941 agcggcaacc ccagcaccct caacagctgg tttcaacaat actttcgctc caccaaattc
     6001 tgaacgaact tcgtgaggaa ttgttgtacc gaccatagga acttcaacca tgtttttctt
     6061 agcagcttca acagctttgc gaatagcttc tggtacttct tgagctttac ctgtaccaaa
     6121 accaacacga ccattacggt caccaacaac tacaagagct gcaaaacgta gacgacgtcc
     6181 acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa gttctactgc
     6241 attatcttta aatgccatta ttaagtgtcc tccctattag aatttcaatc cgttttcacg
     6301 agctgagtca gccaaagctt ttacacgtcc gtgatagaga tatccaccgc ggtcaaacac
     6361 cacttcagaa atacctttag ctactgcgcg ttcagcaaca agtttaccga caacaacggc
     6421 ttgttctgtt tttgttccgt tagaaacttc tttatcaaga gttgatgcac ttgcgagcgt
     6481 tacacccgct acgtcatcaa ttacttgagc gtagatgcct gtattagaac ggaaaatgtt
     6541 caaacgtggg cgatcagcag ttccagagag tttaccgcga acgcgacggt ggcgtttttg
     6601 gcggatttta tttttatctg gtttcgaaat cacaattttc acctcttaaa tatattgtta
     6661 tgtcaaaatg acaatattaa aatgaaaaga tagagaattt gcagaaaaga ttctacaaaa
     6721 tctatcaatc ctattattta cctgttttac cttctttacg gcgtacgaat tcaccaacgt
     6781 aacgaatacc tttaccttta tatggttctg gtgaacgtaa gctacgaacg taagcagcag
     6841 tttgtccaac gctttctttg ttgataccga ttacgttgat tgttgttgga gttggcactt
     6901 caaaagtaac accttctgga gcttcaactt cgtcttggtg agacttacca acagaaagaa
     6961 caagttttga tccttgaagt tgtgcacggt aaccaacacc gcgcatttca agagcttttt
     7021 tgaaaccttc agaaacacca acgaccatgt tgttcaagtt cgcacgagtt gtaccatgaa
     7081 ttgttttcat ttcttttgag tcgttaggac gagtaactgt tacttcagtc ccttcaacag
     7141 tgattccaat atttttatta aactcacgag tgagttcgcc tttaggtcct tttacagtaa
     7201 caacattatc cttgttaatg atttcaacac ctgcaggcaa tgtaattact ttattaccaa
     7261 tacgtgacat gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa ctagttttca
     7321 cggggactca tgagaccaca ggattatact gtgactcaat gagcatttta ggtaaaattt
     7381 taccaaatat atgcaagtac ttctccacca acattttttt ggcgagcttc tttgtcagtc
     7441 aaaagacctt cagaagttga tacaattgca ataccaagtc cgttaagaac tttaggcata
     7501 tcttcatgtt tagtataaac acgaagacct ggttttgaaa tacgtttcaa gttagtgata
     7561 acacgttcac cattttgtcc atatttaagg aacacacgga tgatgccttg tttgtcatct
     7621 tcaataactt caacgttttt tacaaaacct tcacgtttaa ggatatcagc aatccctttt
     7681 ttaatgtttg atgcaggtac ttctaacact tcatgttttg cttggttagc attacgaata
     7741 cgagttaaaa agtctgcaat tgggtcagtc ataaccattt tttaattctc ctcttattag
     7801 cagtttgaat ttaatcactt gctaattaat gagtccttac ggacgttgta agactaggtc
     7861 ttaatatatt tgcgtttaaa aatcagtgcc attatcaatg acacctttta aacacaaagc
     7921 atcattaatt tattaccaag atgctttagt tacgcctggt acttgacctt tataagccaa
     7981 gtcacggaag caaacacggc aaagttggaa tttgcggtaa actgagtgtg gacgtccgca
     8041 tttttcacaa cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt tcttagcaat
     8101 catagatttt ttagccaatt tatttacctc ctagattact ttgcaaaagg cattccaagg
     8161 ccttttagca attcacgtga ctcttcatca gtgttagcag tagtaacgat aacgatatca
     8221 agaccacgta ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa ttgttcttta
     8281 actccaagag tgtagtttcc acgtccatca aatgatttcg ttggaacacc atggaagtca
     8341 cgtacacgtg gaagtgaaac agaaactaat ttatccaaga attcgtacat acgttcacca
     8401 cggagggtaa cttttgcacc gatcgctaca ccttcacgaa gacggaagcc agcgattgat
     8461 ttcttagctt tagtaataag tggtttttga cctgaaataa gtgctaactc agcagcagct
     8521 ttctcaagat ttttagcgtt tgatacagca tcaccaacac ccatgttaag aacgattttc
     8581 tcaacttttg gcacagccat aacagatgaa tagttaaatt tttctgtcaa cgcaggaaca
     8641 acttcattag tatatttttc ttttaagcga tttgccatta tgcttctcct ttccttcgtg
     8701 attaatcaag cacttcgcct gattttttgt tgtaacgaac ttttttgccg tcaacaactt
     8761 tgtaaccaac acgtcctgct acaccatttt tatcaagaac ttgaacgttt gacacatgga
     8821 ttggagcttc tttttcaacg atagcgcctt gagggttttc gttatttggt ttttgatgtt
     8881 tcttgatgag ggctacgcct tcaacaacaa ctttgtttac ttttggaaga gctttaagaa
     8941 ctacagcttc agtgcctttg tccttaccag caataacgcg aactttgtcg ccttttttta
     9001 caaacatttg agtttttctc ctatttttct tacgcctcga aaggcaccct aggaaatcct
     9061 aggggactac gtttgttaat ctgagattaa agtacttctg gtgccaatga aacgatcttc
     9121 atgtagccac cttcacgcaa ttcacgtgca actgggccaa agatacgagt tccgcgagga
     9181 gttttatcgt cacggatgat tacagcagca ttgtcgtcaa atttgatgta tgaaccgtct
     9241 gggcggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc acctttttta
     9301 actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc gatgttagcg
     9361 aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc accgctatta
     9421 tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt ttagtttgat
     9481 tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt agctgaaagt
     9541 gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt ttcatcatgt
     9601 gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt acgtttagtt
     9661 tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc gtaaagagtt
     9721 ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt tcatttctga
     9781 ttgcacagtc ttaacacgtg caatttgttt cttaacttcg tcaaggcgag cagtcttttc
     9841 aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga gttcgttttc
     9901 tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat ctttaatttc
     9961 ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc atttaactgg
    10021 taatttgtgg ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa caccagcaat
    10081 ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg tccctttacc
    10141 agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga agattttaat
    10201 ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag cttcgatttg
    10261 acggtttgta atccagtggc tagttgtagc ttgaagtccg tattcaccga atgaaacttc
    10321 tttaccacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt gtttaacacg
    10381 tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg gaagaacttc
    10441 tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag cttcttccca
    10501 agcatagtcg atatcagcac gaagcgtgtg aagtggaaca gttccttctg aataaccttc
    10561 agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa tacctttagc
    10621 tcctgcacgc attgtacgtt ggattgcttg tttttgagca cgacggaaag caacacgttg
    10681 ctctaattga cgagcaatat tttcaccaac aaggtgagca tctaaatcag gttgtttgat
    10741 ttcaatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac cacgaagagc
    10801 gtcaacgttt gctccacctt taccgataac catacctggt ttagcagtgt gaagtgaaac
    10861 aataacttta tttactgcac gttcaatttc aatagttgaa actgatgcgt cagctaattc
    10921 tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt attctttttc
    10981 agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa ttggatgtac
    11041 tttttgaccc acgattttac ctccttattt ttctgataca actacagtta cgtgagttgt
    11101 acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt tcattgttgg
    11161 tccttcgtta gcgaaagttt cagatactac caagtttgct ttttccaaac caaagttgtt
    11221 ttctgcattt gcaattgctg agttaagagt tttctcaata acacgagctg ctttgtttgg
    11281 agtgaatttt aagattgcga ttgcgtcagc aacattctta ccacggataa gatcaagtac
    11341 taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag ctgaagtaat
    11401 ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag ctgcgtgacc
    11461 tttgtaagta cgtgttggtg caaattcacc aagtttgtga cctaccatgt cctcttggat
    11521 ataaacagga acatgtttac gtccatcata aactgcgatt gtgtatccga tgaaacttgg
    11581 gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt catttgcttg
    11641 agcttcaact tttttcatta aatgctcgtc gacgaaaggt ccttttttaa gactacgtcc
    11701 cattttgtag ttttctcctt taaataatgt accacaacgg cctgtacact atagtgtact
    11761 accgagctgg cggatttgta taagattatg ctattaacct attatttttg gttacgacga
    11821 cgaacaataa gtttgtctga tttagctttc ttattacgag ttttaagtcc aagcgcaggt
    11881 ttaccccatg gagtagatgg tgctttacga ccaactggtg ctttaccttc accaccaccg
    11941 tgtgggtgat cgttagggtt cattacagaa ccacgaactg tagggcgaac acctttccaa
    12001 cgattacgtc ctgctttacc aatgttaaca agtgattgct gttcattacc aacagtacca
    12061 attgttgcac tgcaagtacc aaggatcata cgtacttcac ctgattgaag gcgaacaaga
    12121 acatatttac cttcttgacc aagtacttga gcagaagcac cagcggcacg aatcaattca
    12181 gcacctttac ctggttgcaa ttcaatatta tggataactg taccgactgg aatgttagca
    12241 agcggaagtg cgttaccaac tttgatgtct gcttctggac cagaaataat gcgttgacct
    12301 acttcaaggc ctttaggcgc aaggatgtaa gctttaacac catcagtata gtgtacaaga
    12361 gcaatatttg cagtacgatt tggatcgtat tcaattgttt taacaactgc ttcaacacca
    12421 tctttattac gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc accttggtga
    12481 cgaacagtaa tacgtccgtt gttgttacgt cctgctttat tctttagtga aacaagcaat
    12541 gatttctcag gagtgtttgt agtgatttct gcaaaatcca aagaagtcat attacgacgg
    12601 ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta gattattcag
    12661 cttcagcagc aaacaactcg attgcttttg aatcagctgt aagagtgata atagcttttt
    12721 tagtttttga agtaaaacct gtgtaacgac caacacgttt tgctttgggt ttaactgtca
    12781 cagtgtttac acttgcaact tttactccgt caaatgcagc ttcaacagct tgtttgatca
    12841 aaagtttgtg tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca agagcaacca
    12901 ttgatttctc agtgataaca ggttttttga ttacgtcata caaattcatt atgcaagaac
    12961 cccctcgatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt taacgatatc
    13021 aagaacactt gcagtagttg cagtagcaac tgtaacgttt ggaaggttac gcgctgaaag
    13081 tgcagcaaat tcgttacctt cttcaaggat tacaagtact tttgaatcaa tgctaagtgc
    13141 tgaaagaact gacgcaaatt cagcagtttt tggagctgca aatgaaaggt tttctacagc
    13201 tacaaattta tcttcagcaa cttttgctga ataaactgat ttcaacgcaa ggcgacgaac
    13261 tttttgtgga agtttgtagc cgtatgaacg tggagttggt ccaaagacaa caccaccacc
    13321 acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt tttgacgcca
    13381 tggtttacgt ccaccaccag atactgctga acggttttta accgcgtgag taccttggcg
    13441 aaggctagcg cgttggctga ttacaacatc aaaaacaact gattcgtttg gttcgatacc
    13501 gaagatagct tcgttaagct caactgagct aacttcttta ccagtttggt caaatagttt
    13561 aacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt aactgctgat
    13621 ttgatagtga taagagattt cttagcacct ggtacgttac ctttgatgag gacaacgttt
    13681 ttctctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt accacccata
    13741 cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc catagaacct
    13801 ggacggcgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc atggcgtttg
    13861 ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac atcaccagct
    13921 tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt tttgaattca
    13981 cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc aggtttgtta
    14041 ctcaatactt cacgtttgtc atcaaaacca acctgaactg cttcgtaacc gtctgtttca
    14101 acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac agggatgaat
    14161 tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat tccttttgtc
    14221 atgagaaaat atttcctttt cttattttat ttcaaaaagt tttttacgag cgttttttgt
    14281 gctcagaaat caaattaaag tttgatttct acgttgacac cacttggaag atcaagtttc
    14341 atcaaagcat caacagtttt ttgtgttggg ttgatgatgt ctaccaaacg tttgtgtgta
    14401 cgcatttcaa actgttcgcg agaatcttta tatttgtgag tcgcacgaat aattgtgtaa
    14461 agactgcgtt cagttggaag cggaactggt ccagcaactg tagcacctgt acgtgttgca
    14521 gtttcaacga ttttttctgc cgctgtatca agtgtacggt gttcgtacgc tttcaaacgg
    14581 atacggattt ttttgtttgc catcttttct cctctcgtct atttacgata ataggctagc
    14641 tcctcaagaa aaccaacacg agttgcgtgg caatgcaacc tagcgtgtcg caacctcttg
    14701 catcatagct aaagctgttc atacacagca ccagtatatg atatcaaaaa aaaacagcac
    14761 acgcaagcat tattttattt tttttcaaaa aaaatgcaac catttctggt tacatttttt
    14821 gaatatatcg atgatagcga actttcaaaa acagccacct aaagatatta tctgctatcg
    14881 tagccatcca aaccccaagc aaacctaatt caaaaactat tcctaataaa taaccaagaa
    14941 ctactctgat taaccacatg cctatagtcg ttgtgtaaaa tgggagttta gcatttccta
    15001 atccttgcca agctgctgta taaaccaagg tcccaatagt agcaggtact cctacgaaag
    15061 atagcaaaat aacaatcaaa cttcctttaa tggcagaagg attatttgta aataactgtg
    15121 tcaatggttt gccggcaaga agcataaaac ttgatataag gatcattaga actaagccaa
    15181 tataatacga actttgaata gtttcttcta tagattctct atttttttgt cctacatatt
    15241 ttgcagttag gataattgta gcagttgcga tgccaagtcc tggcatataa ttaaattgtg
    15301 tcaaggtttc tccaatagca tttcccgcta caacatttgt acctaactga acaacaatag
    15361 caactataac aacatctcca gctcgcatca tcagacgttc tcctgctgat ggtaaggcaa
    15421 gattccaaat ttgtgcactg attttccaag tcattctttt tattattttt ttaataggaa
    15481 gatacttcgc aaggatacaa ataccaatta atcttgcgat aagagttgat actgccactc
    15541 cgaccaaacc atagcgccac tcaaaaatag caaaacctga taagactgca ttgaggaaat
    15601 tgacaaaaat actgacatac attggtaaac gaggttgccc ttgcacacgt aaaaatgagc
    15661 ctagggtggt taatattcct aaagtaacaa cacctccacc cacaattgct aaatataaac
    15721 caccaacttg tgcgacactt tttgttgtcc ctaaaagttt aaagaaagtt tgtccaaaaa
    15781 caatcgagat aattcctaat actgccccta tcagtgatgt tagaaaaatg gcttgagaac
    15841 atactgaaat tgcatcatcc ttctcactac ctgctaacga cttggccaat agacttgcta
    15901 tactcgcccc taaagctata aaaatagctt gataaatagt aattatatta ttagcaactg
    15961 aaacacctga tactgctaca acacctaact gagccactag ataattatca actactccca
    16021 tgagcatttg taagatattt tcaatcatag ctggtatagc taactgaata atctctttac
    16081 tataacgcac agtatctcct ttctattacc caggatcatt aaaaagagag tgatgggaaa
    16141 atttctaaaa tgtttctttc tcgctctaaa tattttaaaa caatcgtatt tttattattg
    16201 tttcaaacct agacactctt caaccgtttc tcgcatattt gaggtaggta ctaccattct
    16261 atggattact tcggcctttt gtagatctgt aaccgcctta ggtaaagaaa cacctgacaa
    16321 atcgtttaag attgaaatag cagcgaaatc ctgtatctct tctttatttg taatagcctt
    16381 tgtaacaaca cacgggaact tataaggact tgctgttgac acaattactg ttggagtttg
    16441 atctttagtt tctgtctgat aagcttgata aacagctgat gctacagctg tatgcggatc
    16501 ctctatatac tgatactggt caaataaacg tttgatgtct aattcaacaa attgttctgt
    16561 cgcaaatccc gctacaaagc tctctaaaat atttgcttgc ctagcctcta aggcatactc
    16621 cccagtagtc actaaatctt ccattaattt tttagtcgtt tctgcatcat cgcctaaaag
    16681 gtgaaagatt aatcgttcta gattagagga gaccaaaata tccatagagg gacttgatgt
    16741 cactttaaat tctcgatttt tatcgtaggt ttgtgtctta aaaaaatctg ttagaacgtt
    16801 attatcattt gaggcacaaa tgagtttggt aacaggtaat ccaatttgac tagcataata
    16861 tgctgctaaa atattaccaa aatttcctgt aggtacacta aaattaatta gttgaccaat
    16921 tgagatttcc tttgacttaa ctaattgagc atatgcatat atatagtaaa caatttgagg
    16981 aacaaggcga ccgatgttca ttgaattggc tgatgataac tgcatatgct gctgtgataa
    17041 ttttgatcga agaagggaat tattgaacat ttctttaacc gaagtctgtg catcatcaaa
    17101 atttccttca atcgcaacaa catgggtatt ttgcccggct tgtgttatca tctgcaattc
    17161 ttgaatataa ctaacaccat gtttaggata aaatacaata atctcagttc ctggaacatc
    17221 tgcaaaacct gccatcgctg ccttaccagt gtcacctgat gttgctgtta aaataacgat
    17281 tttatcatca accccttgtt ttttggctgc tgttgttagc aaataaggaa gaatggataa
    17341 agccatatct ttaaatgcaa ttgtaggacc gtgaaataat tcaaggtgat accggtctcc
    17401 aatctttact attggggcta tttcagttgt atcaaattta gtatcataag cttgactaat
    17461 acagtaatcc aattcttgtt cagtaaaatc atcaaaaaaa gctgacaaaa cgagtttagc
    17521 cacttcttgg taggaagcat ccttaagttt agtgaaatct aaatcaactt ttggaaatgt
    17581 gatgggggta aagagtcctc catcgtcagc taatcccttt agaatcgctt gactagctgt
    17641 cactttatta ctagcatcac gtgttgattg atatactatt gccataagaa tccctcttta
    17701 tataaatatc ttttagttat tgtaacacaa tttaataaca tgtccattgt tcgttatata
    17761 attaaccccc ctttattaaa ataaaggggg gcgtaatatc attatcactt tgtaaagtcg
    17821 agaactttac gtccttgaat caatcctctt tccatttcat cgaatacttg aggagctgta
    17881 tccacaggca ctttttcaac cactggtact accaaacctt ctgctccaaa tgcgaaggct
    17941 tcttctaaat ctttacgagt tccaactaag gaaccaacca cacgaatccc atcaagtact
    18001 gtttttacaa tactcaattc catatattct gatggaagac caacagctac cactgtacct
    18061 ccagcacgaa cgctgtcaat cgcctgatta aacgcaactt tagaaacagc agtcacaacc
    18121 actccatgac aaccgcctgt cttttcttga atataggccg caacatcttt tatttctttg
    18181 ccattaactg tcaaatctgc tccaacctct ttagctaatt gaagtttatc tgcgttaata
    18241 tcaacagcta caacatgagc attgaatact ttttttgcat attggactgc taagtttcca
    18301 agaccacctg caccatacac tgcaatccac tgaccaggag cagctccagc ttctttgata
    18361 gccttgtatg ttgttactcc agcacaagtg attgatgatg cttgagctgg atctaatccc
    18421 tcaggaacct taaccgcata gtccgcggta acgatagcgt attcactcat accaccatca
    18481 acactgtatc cagcattttt aacactacga caaagcgtct cacgtcctgt agtacagtat
    18541 tcgcaatgac cgcacccttc aaagaaccat gcaatagaga cacgatcacc aaccctcaaa
    18601 gacgttacgc cttctccaat ttcttctact ataccaatgc cttcgtgacc caaaatgcgt
    18661 cctggtacct ggccgaaatc tccatgtgca acgtgtaaat cagtatggca cacaccacag
    18721 tattctactt ttacaagtgc ttcaccatga ccaacttttg gaagatcatg ctcaacaact
    18781 tcaacacctg tactagcttg attaacaaca accgctttca tgataaaacc tcctagaaat
    18841 aaatatatct tatttgtaat cgtttacatt tttatattgt attattattc acaaactatg
    18901 tcaagtgatt ttcagaaaat tttttgaaaa tattattaac tggttaatct ttttgaaact
    18961 aaaaatccca ctcaatagag tgagattctt cgactgttgt tacttacggc gtcctggacg
    19021 ttcctcataa ccataataag catcttcgat gatttcttgc atgtgatcta ccattggaag
    19081 gcgtggattt gctggtgaac attgatcttc atatgctaag aaagctagtt cacgagattt
    19141 ttctttccat tctttttcat caatgccttg gtcacggaaa ttcatcttaa tacctaaacg
    19201 tgttccaagg tcatacacag cttttgcata tgattcgaca gcttcttctg gtgttgctgc
    19261 tggtaagccg agtaatttag cgatatcttg atacttttca tcagcacgat agtaattgta
    19321 cttaggccat gttgctgttt tagctggacg agttccatta tagcggataa catacggcaa
    19381 aagaattgca tttgtacgtc cgtgtacggt atggaattgt gcgccaattt tatgcgccat
    19441 tgagtgagaa atacctaaga atgcattggc gaaagccata cctgccatag ttgacgcatt
    19501 gtgcatcttt tcacgtgctt caaaatcagc atcttttact gagcgttcta agtagtcaaa
    19561 tacaatttta attgcttgaa gagcaagtcc gtctgtataa tcatttgcca tctgtgaaac
    19621 ataagcctct gttgcatgtg tcaatacatc catacccgta tcagccgcaa taaaaccagg
    19681 aacagtcata acaagggccg gatcaacgat agctacagtt ggtgttaatg aataatcagc
    19741 gattggatat ttacgattat ttgctttgtc agagataacg gcaaatggtg tcacttcaga
    19801 acctgttcca gaagttgttg gaatagcaac aaattttgtt ttcttaccaa gttctgggaa
    19861 tttgaacgca cgtttacgga tatccatgaa tttttgaacg aggtcatgga aatcaacttc
    19921 tggttgttca tagaagagcc acataacttt cgcagcatcc ataggagaac caccacctaa
    19981 tgcaataata gtgtctggtt tgaaggtacg cattaaatca gtccctttca ttactgttgt
    20041 aatgtctgga tctggttcta cttcagcaaa aatctgataa acaactttat tgcgacgaag
    20101 atcaagttgt tcaatgatac gatccaagaa accaagctca accatggcgt gatctgtaac
    20161 aatcatgaca cgttcaacat cgcgacattt ttgcaaatat tgaatagaat cacgttcgaa
    20221 gtatgttttt gaagggactt taaaccattg catattattt ctacgtcttc ctactttttt
    20281 gatattcaag agatttatag cactaacgtt atcaccaact gagttacgtc catacgaacc
    20341 acaaccaaga gtcaatgatg gcaagaaagc attgtaaaca tccccaatac caccaaaagt
    20401 agaaggagaa ttccaaataa cacggatagc acgaattcta gttccaaatt cacgagctaa
    20461 gtctgcatct tttgtatgaa tagcagctga gtgccctaga ccattaaact caaccatttg
    20521 acgagctttt tcaacaccat cttctgtaga ttcagctttc aatactgcaa taacaggtga
    20581 tagtttttca cgtgtcaaag gttctttttc actaacttca gtacattctg cggcaagaat
    20641 atttgttcct tctggaacag taaatccagc ttgttcagca atccaaactg ctgattttcc
    20701 aacaatattt ggatttaatt ttgcaccagc acagtttttg ctatttgctt ttgcaccaaa
    20761 acagaattct tcaagaagag ctttttcttt cttattaacg aaataggtat gataagattt
    20821 aaattcttca acaaattctt tataaatttc tttatcaata ataacagcct gttctgacgc
    20881 acataccata ccgttatcaa atgatttaga cataacaata tcgtgagcag cttgacggat
    20941 attagctgat ttttcaacat aagctggaac attcccagca cctacaccaa gggcaggttt
    21001 cccacatgag taggcagctt taaccatcgc attaccacct gtcgcaagga tagtggcaat
    21061 accatcatgt ttcatcaaag cattagttgc atcaatcgat ggttgttcaa tccattggac
    21121 acaattttca ggagctccag cagcgatagc agcatcacga acaatacgtg ctgcatgtgc
    21181 tgatgattct tgtgctgatg ggtggaaggc aaaaataatt ggattacgtg ttttaagaga
    21241 gattaatgac ttgaaaatag cagttgaagt cgggttggtt gttggcgtaa taccacagac
    21301 aactcccact ggttcagcaa ttaatgtcaa gcctgtgaca tcgtcttctt caataactcc
    21361 cactgtcttt gtatgacgca tgttgttaac gacgtgttca catgcaaata aattttttgt
    21421 cgctttatct tcaaagacac cacgaccagt ttcttctact gcatgaagag caagttctcc
    21481 atgggcatcc aaggctgcaa cagaagcttt agctacaata taatcaactt gttcttgatt
    21541 aagttgacgc atctcatcaa gtgcttttaa accgttttga actaatgtat caattgcaag
    21601 agcaacatct gtagtttcta ccgctttagt tttttcagtc atttgagact ccttttgatt
    21661 tgttaaatgt ttcacaaatt tgtttacgag attattatag catatttaaa aactttgtaa
    21721 atagtttcac aacttttttg tgaacttttt ttcaatcttt cggaaacatt tcttgaaagc
    21781 gctttaatta aatttgtttt ttcattacta attttcagat taaagccaat cgatttcatt
    21841 tgtatcacca taaacggagg tatctattat tcgttttcac aaaaaagccg agtacatgac
    21901 tcggacttgt ttattttgca gctcgttgag ctgatttttg aatagctttt ttgacgttac
    21961 taacatataa atcagcccct gtagtatcgc tatcactata atgcacacca tctgtatttc
    22021 cccagatttc tggatgttct gtcgcaacct tataccagtc agcaaccgtt atgtaattat
    22081 attttttcat tagcgagagt tcataatcac gcactgtcgt aacttgactc atatttttag
    22141 cattataagg cgttactata atcaaacggt gtcccttagg caaagattta ataaattggc
    22201 tcaagtctgt cttgtagtta tctaatgaat tcacaccaac tgccaaaaca acaattttgg
    22261 acaaggcttt attttgaata cggttttcaa aaatatcaaa tgatttacta aaattacgac
    22321 tcaccgcagc atctaattgt acttctggta acaatttatt aaaagcagca cttgacctta
    22381 aggcaactga atctccgatt accgtcacat cactaagagc acctgcatcc cctgcggcta
    22441 gtgtatgtgt tcggttagta tttgttctag cttgttgtaa cgaattctgc aataattctg
    22501 tctcaaaact tcctatactt ggagctgtca acattgtcac tacagtaata agagttagta
    22561 cccctccgat actaaataac cactttttgt aaggcaacaa gtcaaattca taatcaagga
    22621 atttaggttt tctacctaaa ataaaaggtt caatgatata aaatgataat gttgagaaga
    22681 ctgctgataa tactactgtc aaaataacag ctatccagtt tggggataaa cgactaaaaa
    22741 taatataaaa tggccaatga aaaaggtaca taccgtaact gatatcagca agataagtaa
    22801 tcacgaaagg ttctgaaata tctggtgtat gctcgtgtaa aatacgtgca ttgtaaatca
    22861 ttacactagc aaaaatgcta gacaaaacaa aaccaaaaag atatgttagc cgattatcaa
    22921 aatcaagtgc aaaagttaaa atcattagac cagcaaaagc actacccatc atgattaaat
    22981 tatgcttcaa agtcagattt ttgatatttt tcttgaatcg tccggtaatc tctcgaatac
    23041 cagatattgt agccaccatc gctcctaaaa agaacggaaa tatatgtgat aatgtagaga
    23101 aatatatcgt tgaaaaatta tcaacgaaaa aggctctaac aaacatggta aggaagctga
    23161 ctccaaaaac tcccatggaa acaaggaaca aagtccccct caattgcttt tgatcttttg
    23221 atcttttcga taagagccat accgttaagc cccataaaac ataaaaatgg acttcaatag
    23281 aaagactcca agtatgcaca aataagtgag ggataaattg actttcgtag ttaccaccag
    23341 tcaagatttc gtaaaagttg ctggtaaaac ctaaagcagt cataatttga gagccaatac
    23401 ttgctctaaa atcgctttta accagaaata caaaaggtat tgtcaccaaa accatcaata
    23461 ctaatggtgg gaaaatacgg taaaaacgtc tacgacaaaa actaacaaaa tcaatttttt
    23521 tcgttttaga aaattcatca atcaacaagg ccgtaatcag aaaaccagaa aatgtaaaga
    23581 agatgtcaac accaacaaat cctcctggaa aactattctt aaagaaatgg taaagtaaaa
    23641 caagtagtag tcctgttata cgtaccaaag aaaaccattt tattctcatt tttgataaat
    23701 cccctgttta aatgtcccct tatagacttt cttattttta gcattgaggc gaccttgtcc
    23761 atcaggttga ccttttttaa agtcacctgt ataagaccag ccatgaactg aaacaaatgt
    23821 tcctttccct tcaaagaccc catttacaaa atcgccctta tagtaatccc cattctcata
    23881 ggttaattta cctttaccag tcatatggtg attaacaata gagcccacat attttatctt
    23941 accgccatca tagctaagga ctccttgact agaagtcgtt aaggtaaata ctgataaacc
    24001 aaaaacaaca ataattatca aagataatat ttctagatga gttcgtgtta ttttaaaatt
    24061 cttcatttct acttaatctc tactattatt tattagcgtt aaccactgtt tacagccatc
    24121 actgactaga cgataggttt cctcaaaatc accagtatac catggatcag gaacaccacc
    24181 atcacaaaaa aggctaatct tatcatcata ggttccttgt gatagagctt ttaaatcctt
    24241 gacattttgc tcatccatac cgatgatata atcaaaatat gctaaatcgt ctttagaaat
    24301 aggttgtgac cctttatcgt tagtgtaagg aatttgataa cgctgcaaaa tcgcttgcgt
    24361 tcccctatgt ataggatttc catgctccca tgttgaagtc gcgcgactct caaccgagag
    24421 gctatcctcc ttatctaaat tcgccataac aaactcagcc atagggctac ggcagatatt
    24481 tccaagacaa acaaaacaga ctttcttcat agcaaactcc ttttggtcta ttataacaga
    24541 aaaaaggtac ttataatacg tttaactgct caaagaataa ctacaacata tctaatactt
    24601 aacattgcat gaaaattaaa aaattaagac tagataccct agccttaaaa tgacttatgt
    24661 tttatttatc aaatcgctga taacctaaat gttcgtatgc cttatccgta gctacacgac
    24721 cggtacgggt acgcataata aaaccttttt gaattaaata aggttcgtac atgtcttcaa
    24781 cagtatctcg ttcttcagta atattaacgg atagagttcc taaaccaaca ggacctccat
    24841 tatacatttc aatcatggtt cttaagattt tttgatcgac gtaatcaagc ccctcgtgat
    24901 caacatctaa catcgttaat gctttatctg taatattgtc atctatcaaa ccatctccca
    24961 tgatttgagc ataatctcga acacgtttca ataaacggtt agcgatacgt ggcgttccac
    25021 gactgcgacg cgctaattca gaagcagctt cataagtaat tttcatttca aaaatatctg
    25081 ctgtacgctc aataatttct gtcaaatcgt tttcttcata atactccata tgccctgtaa
    25141 taccaaagcg agcacgtaag ggattagata acatacctgc acgtgtcgtt gcaccaatta
    25201 aggtaaaggg cggcaaatct aaatgaacac ttctactagt ttctcctgca ccgatcataa
    25261 tgtcaatata gaagtcttcc attgcactat aaagtacctc ttcaaccgcc atcggcatac
    25321 gatgaatttc atcaataaaa agaacatcac ctggttctaa atcatttaaa atggctacta
    25381 aatcccctga tttttcaatt gcgggacctg atgtttgttt gagattgaca cccaactcat
    25441 tagcaattac aaatgccatg gttgttttcc ctaaaccagg agggccaaat aataacacat
    25501 gatccaatga ctcatcacgc aatttagcag cttcaataaa tatttttagc tgatctttaa
    25561 ccttatcttg tccaatatac tctcttaaat actgcggacg aagtgtacgt tctaccaatt
    25621 cttcgtcacc cattgcatca ctatctaaaa atcttgtcat gatattatta taacatttgt
    25681 ataggaaaag ttaagaacta agttggtgta ttagtatctc ccttatttaa aggaagaata
    25741 aactgatctt atagttaatc caacctcaag aaatcacaga acagcttact tagcccatat
    25801 tccaccaacc cattcctaaa aacaaatcaa atgaacctaa atttgataat agaacaagta
    25861 atactattaa aaaacatttt ttaatcacta taataaactc ctaaataata ttttgtacgt
    25921 ttattataaa tttttagacc tcagagtcac aggacatcaa tgtcctgtta tatcttcttg
    25981 ccactccaat tctaattttt taattagatg cttgtcatca atcatttttg aaaacataat
    26041 tgattgttgg taagcatttt ctgcagactt tatatcattg tcaattgtaa tgaagtattt
    26101 ccattggtac ataaaatata aaggcatttt atgaaaatca tgtgttgaca ccatcgtttg
    26161 tcggctaact tctaacattt ctgcaagaca ctcttcacta tttaaacaaa gtccaacaaa
    26221 tacacaattc aggagtacat gattaaacca aaacaaatct tctgctgtaa gtttgtggtt
    26281 ctgacttatc aaagttttac atatagtttg aaatacttct ttagtaaatg ctctattgtt
    26341 aaatttagat acaactgcac aagttaaaaa caaatctatg ataatcaaat cattaagagt
    26401 atatttttct ttatatttta ctttatcaaa gcattcgcat aaaacctcca caccaaaatt
    26461 aatgtctcct gtttgataga cttcaaattt agcttgtaaa ctgtcaattg ctaagcactc
    26521 gtc
//
LOCUS       DATROY010000011        23439 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.011, whole genome shotgun sequence.
ACCESSION   DATROY010000011 DATROY010000000
VERSION     DATROY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23439
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            691..2109
                     /locus_tag="VBD11_000449"
     CDS             691..2109
                     /locus_tag="VBD11_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO6953426.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            2380..3339
                     /locus_tag="VBD11_000450"
     CDS             2380..3339
                     /locus_tag="VBD11_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6953427.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            3500..4402
                     /locus_tag="VBD11_000451"
     CDS             3500..4402
                     /locus_tag="VBD11_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6953428.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            4562..5626
                     /locus_tag="VBD11_000452"
     CDS             4562..5626
                     /locus_tag="VBD11_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6953429.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            5741..6733
                     /locus_tag="VBD11_000453"
     CDS             5741..6733
                     /locus_tag="VBD11_000453"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6953430.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(6778..7227)
                     /locus_tag="VBD11_000454"
     CDS             complement(6778..7227)
                     /locus_tag="VBD11_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO6953431.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            7193..7897
                     /gene="rsmD"
                     /locus_tag="VBD11_000455"
     CDS             7193..7897
                     /gene="rsmD"
                     /locus_tag="VBD11_000455"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6953432.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            7909..8199
                     /locus_tag="VBD11_000456"
     CDS             7909..8199
                     /locus_tag="VBD11_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953433.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            8196..8681
                     /gene="coaD"
                     /locus_tag="VBD11_000457"
     CDS             8196..8681
                     /gene="coaD"
                     /locus_tag="VBD11_000457"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6953434.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            8671..9744
                     /locus_tag="VBD11_000458"
     CDS             8671..9744
                     /locus_tag="VBD11_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6953435.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            9819..11153
                     /locus_tag="VBD11_000459"
     CDS             9819..11153
                     /locus_tag="VBD11_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6953436.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            11222..11800
                     /locus_tag="VBD11_000460"
     CDS             11222..11800
                     /locus_tag="VBD11_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6953437.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            11851..12957
                     /gene="rlmN"
                     /locus_tag="VBD11_000461"
     CDS             11851..12957
                     /gene="rlmN"
                     /locus_tag="VBD11_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6953438.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            12957..13472
                     /locus_tag="VBD11_000462"
     CDS             12957..13472
                     /locus_tag="VBD11_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6953439.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            13668..15413
                     /locus_tag="VBD11_000463"
     CDS             13668..15413
                     /locus_tag="VBD11_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6953440.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            15403..17142
                     /locus_tag="VBD11_000464"
     CDS             15403..17142
                     /locus_tag="VBD11_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953441.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            17270..17836
                     /locus_tag="VBD11_000465"
     CDS             17270..17836
                     /locus_tag="VBD11_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6953442.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(17904..18442)
                     /locus_tag="VBD11_000466"
                     /pseudo
     CDS             complement(17904..18442)
                     /locus_tag="VBD11_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(18443..19435)
                     /gene="bioB"
                     /locus_tag="VBD11_000467"
     CDS             complement(18443..19435)
                     /gene="bioB"
                     /locus_tag="VBD11_000467"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6953443.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            19560..20075
                     /locus_tag="VBD11_000468"
     CDS             19560..20075
                     /locus_tag="VBD11_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953444.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            20065..21180
                     /locus_tag="VBD11_000469"
     CDS             20065..21180
                     /locus_tag="VBD11_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6953445.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            21173..22402
                     /locus_tag="VBD11_000470"
     CDS             21173..22402
                     /locus_tag="VBD11_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6953446.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            22515..23147
                     /gene="nth"
                     /locus_tag="VBD11_000471"
     CDS             22515..23147
                     /gene="nth"
                     /locus_tag="VBD11_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6953447.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(23148..>23439)
                     /locus_tag="VBD11_000472"
     CDS             complement(23148..>23439)
                     /locus_tag="VBD11_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6953448.1"
                     /translation="TISTIILTPITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGL
                     SLPLHQMLFIIQIGAWLGIIYCLVMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         7601 a         3613 c         4687 g         7538 t
ORIGIN      
        1 atacatgagg actttataaa tagcaggaaa gtagatacta gttagatata gtagtgcaag
       61 atagatgaga aaaaataacg aatactttaa cttggtcata acaattccat tctaatggta
      121 gtaagcatgc ataaaatgta ggttttatca tactacttga atccagcaac tagatagagg
      181 gggttacata cgataagaca gtagtatttt aaaccgatcg gttgacttat tgatactgtt
      241 tagggaacaa gtgttttgtt gtcaatgtta aatttaattt agcattttga tggtatcacc
      301 gcaagaatga aaggaaaagt tgtatgtgct gaaaaaacta atgcttggcg atttagaaca
      361 cacgtttact agtcgagatg acaaaaaata agtaccaaat gtaaaggtcg atattatcag
      421 cattgtcagc gttaggtgtt ataaaaattt aacatcttcc aattaagcta ggtttaagtt
      481 tcctaagttg aactacgcct agccgaaaaa taatttcttt tttacagaac tcctaacaag
      541 gattttgaat tgtcaagatt cttgtttttt tggaaagtca tttagaggct ttttggggga
      601 agtattaact attgtatccg tttacaattc ctttcaaaaa tgttagtata tcattgaggg
      661 atatgagtta tggataaggg agagaggatg atgatgacta aggaaacgtt ggcacattat
      721 caaaagaaaa tagaacaaga atctgaaaaa aagcagtctt tagatgagca ctcatggcat
      781 gtggcctgtt ttagtcgaca ggaagcatct ataattggtc aaggagatgt gcttttttta
      841 attggtctat accacgatct aggcaaagct gatcgagcct ttcaagataa attattaaat
      901 aatccaaatc gacatgttga tcattcttat gcaggggcaa aatacttatg ctctattatt
      961 gggcctcatc taaaaagtcg aggggttgat aaaggtgaga gaatgacatt caatgaaatg
     1021 gtgggttatg tcatctcggc tcatcatggg atgtatgatt tttgctactg ttctgacgat
     1081 gctgaatatt atagctttaa taaatttaaa aaccgtatca acagagactt agatgattat
     1141 cactatcacg aagatattaa agggtatgct ataaaattag aagaaaaatt gtgtgattac
     1201 ggctacaaag atttaaggga gcttattgat aaagcttttg ataattacca acaagctatg
     1261 tcttccttaa actggcaaga taatagtgag tgggattatt atcagtcttg tatggtgaga
     1321 ctttacttgt cgctcttaaa aaacgctgat attttggaca cagtaaatgc ctatggtctt
     1381 aaaataagtc ctatggataa aacagagcga tcctctctaa aacattctta tttagcagcc
     1441 attgaacaaa aatatgctag ctttggacga ccaaacaatc agttgaatac tattcggaca
     1501 gaaattgctg agcgtgttaa agaaagaggt aaacgggatt ccaaagggat ctatcgctta
     1561 gatttaccga caggagctgg caagactaat cttagtatgc gctatgcgtt tcaccaatta
     1621 gttcatcaag acaaatcaag atttttttac ataacgccct ttctttcggt tcttgagcaa
     1681 aatgcttccg aaattagaaa agttacgggt gaccttggcg ttctagaaca ccattccaat
     1741 atggttaaac aggctaatga agatgatgac aaggacagtt tattgtcagc ttatcttatt
     1801 gatagctggg atagtcaagt agtcttgact tctatggttc aatttttcca aacgcttttt
     1861 aaaacaaaat cggctaattt gagacgtttt tcaagtttga ttaatagtgt tgtgattcta
     1921 gatgaagttc agtcactgcc tattgaagtc accactttgt ttaatttaac gatgaatttt
     1981 tttaataaag ttatggatac aaccatcgtt ctttgtacag caacacaacc tgcttatgat
     2041 tcttcagaga ttgaccatcg tatctgttat ggagggaatt tgggagaatt agctgaaata
     2101 gtggattgaa ataagtttat acgttgcttc atcaccaagt tttacgtcgc acccattgcg
     2161 ggtgcgtgga ttgaaatata agttcagcaa cttcacgaga aaatgttcct cgtcgcaccc
     2221 tttgcgggtg tgtagtttca actattttaa atctttcaaa ttatgctatt aaaattgtct
     2281 aacttttaac agagatgcga ttgatttgta ttaatcccta aaaagataaa ttgctagttc
     2341 ttatgctata ataactctat caatgtcaaa ggagttttta tgttaaaggt aagatatggt
     2401 gttgtgtcaa cggcaaaggt ggcgcctcgt tttattgaag gagtccgctt agcaggaaat
     2461 ggtgaagttg tggctgtatc gagtcgaact cttgagtctg cgcaagcttt tgccaacaaa
     2521 tatcatctcc caaaggctta cgataagtta gaggatatgt tagcagatga gtcgattgat
     2581 gtgatttatg tggcaacaat taatcaagac cattataaag ttgctaaagc agctctgtta
     2641 gcgggaaaac atgtgttggt agaaaagcct ttcacattga cttacgacca agcaaatgaa
     2701 ttattcactt tggcagaaag ttgtaatctg tttttgatgg aagcacaaaa atcggtattt
     2761 attccgatga cacaagtgat taaaaaatta cttgcctctg gtgaaatagg agaagtgatc
     2821 tccatctcat ctacaacagc ctacccaaac attgaccatg taacatggtt tagagaactt
     2881 gagttaggtg gaggaacagt gcattttatg gcgccctatg ccctgtctta tttacaatac
     2941 ctatttgatg cgactataac tcatgctagt gggactgcga cttttccaaa aggtcaaagc
     3001 gatagtcaat ccaaactact cttgcaatta tcaaatgggg ttctcgtaga tatcttcttg
     3061 acaacacgtt taaatttacc gcatgaaatg attatttatg gaactgaagg tcgtctaatc
     3121 attccacatt tttggaaaac cactcatgca aaattggtga gaaatgatac tagtgcgcga
     3181 acgattcaag ttgacatggt tagtgatttt gagaaagaag cttatcatgt tagtcaaatg
     3241 attttagaag ggcaaagggt aagtcatatc atgacgcctc agttgacgct gtcaggtgtt
     3301 aagattatag aaggcttata ccgttcatgg gggaaatgat gctggctatc tagcatatta
     3361 ataaggagta ctagatgtta ttttagtagt ccttttttgt tttgctctca taaggttaat
     3421 tagatttaaa agggttttct acttgttttt tctcgatttt tgtttcataa tgtagacaag
     3481 tttagagtga ggtgcttcta tgttttcgag cgattttgga tttgagataa ttaatgttga
     3541 cgatctatcc ttacaagagg agaaattttt accttcagaa ttgctggctt atgcacgtga
     3601 tgaaaacgag tcttctttcg tacgtgatat tgaggggcat ttgacacttg tttaccagct
     3661 tttggatacg caaggtcacg ttgatgatgt tcgtcatgtg ccgagagtga taccagtgac
     3721 tctatttttg aaagaagatg gcttgtttgt attagctaat cataaaaata ttatccttgt
     3781 aaaaaaagca cttaatcgcg ttgaaaaggt agattcgccg aaacacctct tattatcatt
     3841 agtgactgct ttctcaaagc aatattttga tgttttagat actatttctg aagaaagaga
     3901 taaactaatc aatgatttgc gtaaaagacc aaacaaatcg aacttggcaa ggctggcaaa
     3961 tttacaaagt ggaacagtac acttaatgat gggaactaag caaaattttg aaatgctgac
     4021 cgatttacaa aacattgagc aagataaaga gaatactaga aatgaaaaga tgcaacttca
     4081 agatgctatt attgaagcaa gacaattatc taacatgtgt tccttaaatt cacaggtttt
     4141 ccaagaactt tcttcttctt ataataatgt cctcagcaat aaccttaacg acaatgtaac
     4201 taccttaaca attatttcca ttggtatttc cattattgct atggttacca gcttttatgg
     4261 aatgaatgtt aagttgcctt ttgatagtgt tgatgctgtc tgggtattga ttattttaat
     4321 tacgactatc attactatta tgctctctat tgttatgtac atttatgtcc ataaagagga
     4381 taatcatccc aaacataaat agagaattaa taagagggtg ctatcaatta gtttagtttt
     4441 aagcatggca acatgctttt tttgtacaaa gaaactagaa ttcatctttg actttgagct
     4501 tgctttctaa ctcttaaaac gacataatag aggataatgt acagtgaata gagaggttac
     4561 tatgtcaata gaatcagatt ttttccggaa aaaacgtttt attttttcat cgcttgagga
     4621 gtttggattt atcaaatctg accaagagta tatctattgt caaacattta tggataatga
     4681 ctttaaagct attatcacga tttcattgga tggaaagata gctggtaagg ttattgattc
     4741 ggcacttgaa gaagaatatt taccgcttcg agcagcaaat tataatggtt cgtttgttgg
     4801 cgaagtacga tcggcttata tggctatatt aggtgatatt tcagattctt gttgtaagga
     4861 tttattattt accaaagatc aaagtaatcg tttagcggag aagatagcta caacttttga
     4921 agattcagtt gattatccat ttgcgaagca cccacaatat gcttcttatc gtgtatcagg
     4981 taaatggtat gctcttcttt ttccattgaa gatggggaag ctagaaaatg ttccagctca
     5041 actgtcagaa gaagaggttg aagttttgaa tattaaagtc aatcctcaag atatggaaat
     5101 actcttacaa aaagaaggta tttatccttc ttatcatatg tctaaaaaga cttgggtatc
     5161 tatcgtttta gacaatactt tgtctgatat agaaattttt aaattggtat cagatagtcg
     5221 taagcttgtt tctcccaata aaaaatcaaa ttctgagcca gagttttgga ttattcctgc
     5281 taatcctaaa ttttacgata ttgataagga gtttgcagag catcaattga ttgattggac
     5341 tcaaaaagga aagattgcgg tgggagatta tgtgtttatt tatattacag cacctattcg
     5401 tgctttgcgc tatgtctgtc aagtgataga acgtgacctt aaaacaagta cttacttagg
     5461 agcaagagat gtcaaagctt atatgcgact caaactgata aggcaatttg aagattctac
     5521 ctatcctgta gcatttttag cagaacacgg cgttaagact gtgagaggac cacggcgaat
     5581 gaccaaggaa ttgcgacaag tcatggcaaa aacaattcta gagtaatgac tttcagaaaa
     5641 aaacttgtaa aaaagtcaga ataattctac aattcctctt ccattttgtt acttgaagga
     5701 gtataatagc tctaacattt tataatttaa ggaggaacat atgaagaaaa gctttattca
     5761 tcagcaacag gagatttctt ttgtcaaaaa tacttttaca caatatttga ttgataagtt
     5821 agaaatcgtc gaagtgcaag gacctattct tagtcaggtc ggggatggta tgcaagataa
     5881 tttatctggt attgaacatc ctgtatcggt gaaagtcctt aatatacctg aagctgaatt
     5941 tgaagtcgtt cactcattgg ctaaatggaa acgtcatact ttggcaagat ttgggtttaa
     6001 tgagggagaa ggtcttttcg ttcatatgaa agctcttcgt ccagatgaag attctcttga
     6061 tccaacacac tcagtttacg tagatcagtg ggactgggag aaggtcattc ctgatggtag
     6121 acgtaatttg gactacctaa aagaaactgt ggaaaaaatc tacaaagcta tccgcctaac
     6181 agagcttgct gtggaagctc gttttgatat tgaatccatt ttaccaaagc gtattacctt
     6241 tattcataca gaagaattgg tagaaaaata cccagactta agccctaaag agcgtgaaaa
     6301 cgccatcgct aaggagtatg gcgcagtctt tttgattggt attggtggag agttagcaga
     6361 tggtaaacca catgatggtc gtgcaccgga ctatgatgac tggacaacac catcagaaaa
     6421 tggttttaaa ggtctcaatg gtgatatttt agtatggaac gaacagcttg gaactgcctt
     6481 tgaactatca tcaatgggaa ttcgagttga tgaagatgct cttaaacgtc aagttgttct
     6541 caccggtgat gaagatcgtt tagagtttga gtggcataag acgctattac gtggtttctt
     6601 ccctctgact attggtgggg gaattggtca atcacgatta gctatgttct tgcttcgtaa
     6661 aaaacatatt ggagaagtac agtcaagtgt ctggccaaaa gaagtcaaag ataccttcga
     6721 aaatatatta taaaaaatca gccaagcagt tttgtttggc tgattttaac aataagttta
     6781 tttttggctt aagaaagttt ggatttgttg aggtgtagct tggcttcctt gaacaagata
     6841 tttagtcatt tttcctcgtt gataatgtgc taaatttgga acagtggtaa tatgatgctt
     6901 tttgaaaaaa ctcttcaatc gtttcccttt gtatttttga ctatcaaggt agtaaatttt
     6961 tgaatgaagt gtggcttgag attttaataa ttttggtaag aaagctctac aatattggca
     7021 agttggacgt cctataaaaa tagtcgcttc agattgttga cttttgctta ctaaggcctt
     7081 atctgtgatg gcgattaagt cactatttgt tatcatgtgt tggtattgtt gttgagaaat
     7141 agtcatggtg tcagcgctac ttaagtttga ttttagcgtt gtaaggcata atatggctat
     7201 taaagttaat aataattttt taatcattat gggaacctcg tttctcttac tgctagctta
     7261 tcatatcaaa aaagaaggaa aagagatact atcttctctg tttgttataa gcaatttttt
     7321 tgataactct accttatttt ttgttataat agtggttatg agagtagtag caggaacatt
     7381 tggagggcgt cctttaaaaa cgcttgatgg aaaaacaact cgtccaacaa ctgataaggt
     7441 taaaggcgct atttttaata tgattggtcc tttttttgaa ggtggtcgtg ttttagacct
     7501 tttttctggc agtggtagct tagctattga ggcgatctca aggggaatgg accaagctgt
     7561 cctagttgaa aaagataggc gtgcgcaggt cgttattcaa gaaaatattg caatgactaa
     7621 gagtccggag caatttcaat tattaaaaat agaagccaac cgtgctttag aacaattaac
     7681 gggacaattt gatttggtct tgttggatcc gccatatgct aaggaagaaa ttgtaaagca
     7741 aatccaaatc atggatagca agggtttatt aggcgatgat atcatgattg tttgtgaaac
     7801 ggacaaatca gttgatttac ctgaagaaat tgcttctttt gggatatgga aacagaaaat
     7861 ttatggcatt tctaaagtaa cggtctacgt tcgttaggag gctttagtat ggattacaag
     7921 ttagtgatga ttattctcat cttagtcata ttgatttatt tgttgttgcg ccttcactat
     7981 ttagaagagt tgctaaaaca ttatcacttc aaaaatgatg aaggagtttg ttcattatca
     8041 agaacaaaaa atccattgta tctattaatg gcaatgatta cttttttgcc aaatcttatc
     8101 aattggatag ctaacggttt tgaggcaata gacattgttc gcctgattcc tgtggtagtc
     8161 tttttatggt tgtattattg tggagatgac gacgaatgac aaagaaagct ttatttacag
     8221 gttcatttga tcctgttact aatggacatt tagatattat tgagcgcgcc agctatctat
     8281 ttgatcatgt ttacattggg ttatttcata accttgagaa gcaaggttac ttttcgattg
     8341 agtgccgtaa aaaaatgtta gaagaagcta tccgtcagtt taagaatgtt tcagttttag
     8401 tggcgcaaga tagactagct gttgacttag caagggaagt gggagcaaaa tattttgttc
     8461 gaggtctgcg taatagccag gattttgatt atgaagccaa tttggaattt tttaataaac
     8521 agttggcaga tggtattgag acagtttatt tatcaacgtc gccatctttg agtccgatat
     8581 catctagtcg catccgtgaa ttgatacatt ttaaggcttc tgtgaagcct tttgttccaa
     8641 agagtgtttt tagagaagtg gagaaaatga gtgaagaata aagatccaaa aagaaaacat
     8701 aaaagtttgc taggtctgct taaatggtgg attataggtt ttgctttttt attgttagta
     8761 ttagcaagcc ttgtggtgag gctaccttat tatttggaaa tgccaggtgg agcttatgat
     8821 atccgttcgg tattgaaagt gaataaaaag gctgataaag ctaagggttc gtacaatttt
     8881 gtagcggtat ctgtcagtca agcaacgcca gctcaggttc tttatgcttg gttaactcct
     8941 tttacagaat tatctagtaa agaggagaca acaggtggct ttagtaatga cgattatctc
     9001 agaattaatc aattctacat ggagacttct caaaatgagt ccatctatca agctttaaag
     9061 ttagctaata agcaagtatc tcttacttat aaaggagttt atgtgcttaa cttagctaaa
     9121 aattcgacat ttaaagatag attacattta gctgatacag tcacaggagt taatggcaaa
     9181 agtttcaaaa attcttctca acttatcaaa tatgttgcag ctcttcatct aggtgataaa
     9241 gtaaaggtac aatacacaag tcaaggtaaa aagaaagaat cagttggtaa agttattaaa
     9301 ctatcaaacg gtaaaaatgg tattggtatc ggtttgacag accatacaga agtatcatct
     9361 gatgttcctg tggactttaa tactgaaggt gttggaggac caagtgcagg tttaatgttt
     9421 actttagcga tttacgacca attagttaaa gaagatttac gtaaaggacg taagattgca
     9481 ggcactggta ctattgagca aaatgggcat gttggcgata ttggtggagc aggtttgaaa
     9541 gttgtttcag cggctaaaaa aggaatggat attttctttg ttcctaataa tccgatcgat
     9601 aaaaatgcta aaaaaggcaa aacaaaggtt cagacgaatt atcaagaggc aaaagcagca
     9661 gctaagcgat taggtacgaa gatgaaaatt gtaccagttc aaaatgttca gcaagctatt
     9721 gattatttga aaaaaacaaa ataaactcaa agagttcggg cgtctgctcg gcttttttgt
     9781 tttgcgacat ttaactatca aagtgctaaa atgatagtat gacagagctt ataagaatac
     9841 tacatttaaa tgatctgcac tctcattttg agaattttcc taaggtaaaa cgattttttc
     9901 ataacaatca agcgcagcca atagagacga tttcacttga cttgggggat aatattgata
     9961 aaagccatcc tttgacagaa gcaagctcgg gcaaggctaa tgtccaattg atgaatgaac
    10021 tgggtattga gcttgctact attggtaata atgaaggagt tggtttatcc aaaaaagact
    10081 tggaccaagt ctacaaagat tcagatttta cggttattgt tggtaatcta aaagataaca
    10141 taatcgaacc gagttgggca aaaccttaca ttatttatga gacacagcaa ggaactaagc
    10201 ttgctttttt agcttatact tttccttatt ataaaaccta tgaacctaat ggatggacga
    10261 tagaagatcc aattgactgt cttaaatgcc atttacaaat aaatgagata aaagaggcaa
    10321 attgtcgtat tttgatgagt catttaggta ttcgttttga tacaaggatt gctcaagaat
    10381 tttcagaaat tgaccttatt ataggtgcac atacacacca tttatttgag gaaggggagc
    10441 taattaccgg tacatatctt gcagcagctg gtaaatatgg tcgctttgtt gggagtattg
    10501 atataacttt tgataatcat acactaaaag atattttaat ctcaacatat gatacaaaac
    10561 aactaactgg ttatccttcg gacagtgatt gggctaagga gattgagtca gaaggtaaag
    10621 aacagcttag aaaagaaagt ttaatatcct ttccagcacc attatcatta gaagaatcgt
    10681 gtcaactagt catggatgcg atgctgtttt acgcaggtgc agatgttgct attatcaatt
    10741 ctggcttaat tgttcagcca tttgaaaaag acttttcgag gaaaaatctg catgaatcat
    10801 tacctcatca aatgcgtctc gctaaattga cagtgagtag tcaagaactt ttagagattt
    10861 atgaaactat ttatcaacaa ggtcaatttt tagcgcaaca aaagattcat ggtatgggct
    10921 ttcgtggcaa gtactttgga gaagttttac attcgggatt tgattacaaa aatggaaaaa
    10981 tagtgtataa tgagaaagat attgatgcca aagaagaagt gattcttgta atcgttgatc
    11041 agtattattt tgcatcgtat tttgagtgtt taaaaacaaa aaaagtagac ttgctttttc
    11101 ctgaattact cagagatgtg gtagcagatt atttaataaa tatgacacta taaatgaaaa
    11161 atagataaat aaagagtaga agctagtcgt ttctctcaat gattttagag aaaggagtat
    11221 tatgcgaaaa gaagtgacac cagagatgct taactataat aagtatcctg gcccacagtt
    11281 tattcacttt gaaaatatcg ttaaaagtga tgatattgaa tttcaacttg ttattaatga
    11341 aaaatcagct tttgatgtga ctgtctttgg acaacgtttt tctgagattt tattaaaata
    11401 tgattttatc gttggcgatt ggggtaacga gcagttgagg ctaagaggct tttacaaaga
    11461 tgctagtacg attagaaaaa atagccggat ttcacgttta gaagattata ttaaagagta
    11521 ttgtaacttt ggttgtgctt attttgtgtt ggagaatcca aatcctagag atattaaatt
    11581 tgatgatgaa agacctcata agcgtcgtaa gtcaagatcc aaatcacaat catcaaagtc
    11641 acaaactaga aataatcgtt cccagtcaaa tgccaatgct cattttacaa gtaaaaagcg
    11701 taaagacaca aaacgccgtc aagaacgtca tattaaagaa gagcaagata aggaaatgac
    11761 ctctgcaaag cagcattttg ttattcgtaa gaaaaaataa aaaaaggata aactgtaatc
    11821 gtttattcca ccatgaagaa agaaaatatt atgcctaaaa aaactgatac acccgactat
    11881 aaaccctcta tttatagctt aactcgcgat gaactaatag cttgggctat tgaacatgga
    11941 gaaaagaaat tccgtgcttc acaaatctgg gactggctct ataaaaagcg tgtgcaaagc
    12001 ttcgatgaaa tgactaacat ttccaaagat tttattgcac ttttgaatga gaattttgtt
    12061 gtcaatcctc taaaacaacg tattgttcaa gaatctgctg atggaactgt taaatacctt
    12121 tttgaattac ctgatggcat gctgattgag acggttttga tgcgtcaata ttatggttta
    12181 tcagtatgtg taacgactca agttggttgt aacattggtt gcactttctg tgcaagtggt
    12241 ttaataaaga aacaacgtga tcttaacaat ggcgaaatca ctgctcaaat tatgcttgtt
    12301 caaaaatatt ttgatgaacg tggacaaggt gagcgcgtta gtcacattgt ggtcatggga
    12361 ataggtgaac cctttgataa ctataccaat gtattaaaat tcttgcgtac agttaatgat
    12421 gataatggat tagctattgg tgcacgtcac attacagtgt ctacttcagg tttagcacat
    12481 aaaattcgtg aatttgcaaa tgaaggtgta caagttaact tagcagtatc tcttcatgct
    12541 ccaaataatg aattacgatc aagtatcatg cgtattaatc gttctttccc tcttgaaaag
    12601 ttgtttgcgg ctattgaata ttatattgag acgactaacc gtcgtgtaac atttgagtat
    12661 attatgttaa atggtgtcaa cgatacccca gaaaatgctc aagagttagc agatttaacg
    12721 aaaaagattc gcaaattatc ttatgtcaat ttaattcctt acaatcctgt ctcagaacat
    12781 gaccagtata gtcgtagtcc taaagagcgt gtggaagctt tctatgacgt tttgaagaaa
    12841 aatggagtta attgtgttgt tcgtcaagaa catggtacgg acattgatgc agcttgcgga
    12901 caattacgtt caaacacaat gaaacgcgat cgtcaaaaag cgaaggtagg gcggtaatgc
    12961 ttaaaagatt atttactgaa gatggggaat tgacaaagat tagtcgtcgt ttcgtttgga
    13021 tgttagtggt tatctattgt cttattattg tcaggatgtg ttttgggcct caaattatga
    13081 ttgagggggt atcaactccg aatgttcagc gcttcggaag aattgtagct cttttagtac
    13141 catttaattc ttttcgtagt ttagatcagc taactagctt taaagagatt ttttgggtta
    13201 ttggtcaaaa tgtagtgaat attttactgc tgtttcctct cattataggg ttactatccc
    13261 taaagccaag tttacggaaa tataaaagcg ttatattact tgctttcttg atgtctcttt
    13321 tcatagagtg tactcaagtt gttttagata ttttaataga tgctaatcgg gtttttgaaa
    13381 tcgacgatct atggacaaat accttaggcg gtcctttcgc cctatggagt tatcgaaaca
    13441 taaaaggttg gcttctaact attagaaaat gaaaataatg taaaggctat gatattgttc
    13501 aagcctttta ctatttaaaa aggatcactt tagttgacga aagtaagctt atagaggcat
    13561 aatagagagt tgttaaatga gaattttaaa acacttttta tgtcttctga ttgatttggt
    13621 cttctgactt aattatgatg tttttcaacc tttgagaaag gaaaaatatg tctattataa
    13681 aaaatttgtg gtggtttttt aaggaagaaa agaagcgcta tcttattggg attttgtcat
    13741 taagtttggt tgcagtgtta aatcttatcc ctcctaaaat catgggatca gttattgatg
    13801 ctattacaac tggaaaatta acaagaccac aattactatg gaatttatta ggtttggttt
    13861 tgtcagcttt agctatgtat gggctgcgtt atatttggcg tatgtatatt ttagggactt
    13921 cttacaaatt aggccaagtt gtcagatacc gtttatttga acattttaca aaaatgtctc
    13981 cttcttttta tcagaaatat cgtacaggtg atttaatggc gcacgcgacc aacgacatca
    14041 attctctaac acgtcttgca ggaggaggag ttatgtcagc agtggatgcc tctatcacag
    14101 cattagtaac gcttatcacc atgttcttta ctatttcgtg gcaaatgaca ttaattgcgg
    14161 ttatcccttt gcccttaatg gccttagcaa ctagtaaatt ggggcgaaaa acccatgaaa
    14221 ccttcaaaga atctcaggca gccttttcag aattaaataa taaagtgcaa gaaagtgtct
    14281 ctggcgtcaa agtgactaaa tcatttggtt atcaagaaca agaaattgct tcttttcaag
    14341 agattaatca gatgactttc gtgaagaaca tgcggaccat gacttatgat gtcatgtttg
    14401 atcctttagt tcttcttttt ataggtgcct cctacgtatt aacattggct atgggagctt
    14461 ttatgatttc aaaaggtcaa gttactgttg gtgacttggt aacatttgtg acgtatttag
    14521 atatgttggt atggcccttg atggcgattg gtttcttgtt caatatggta cagcgtggta
    14581 gtgtttctta taaccgtatt aatagtctac ttgagcaaga atcggatata actgatcctt
    14641 taaatcctat caaacctgtt gtcaatggaa cattaagata tgatattgat ttctttagat
    14701 acgacaatga ggaaacctta gccgatattc atttcacctt agaaaaaggt caaaccttag
    14761 gtttggtagg tcaaacggga tcagggaaga caagtcttat taagttattg ctacgtgaac
    14821 atgatgtgac tcaggggaaa attactttaa ataaacatga tatacgtgat tatcgattgt
    14881 ctgagttacg tcaactaatc ggttatgttc ctcaagatca gtttttattt gctaccagta
    14941 ttttagaaaa tgttcgcttt ggaaatccaa ctctatctat caatgctgtc aaagaagcaa
    15001 ctaaattggc acatgtttac gatgacattg aacagatgcc agcaggattt gagactctaa
    15061 ttggagaaaa aggagtctca ttatctggtg gacaaaaaca aaggattgcg atgagtcgtg
    15121 ccatgatttt agatccagat attcttattt tggatgattc tctatcagca gtggacgcta
    15181 aaacggaaca tgctattgtt gagaatctta aaacgaatcg tcaagggaaa tcgactatta
    15241 tttcagcaca tcgtttatca gctgttgtgc acgcagacct tatcttagtt atgcgagacg
    15301 gcagagtcat tgagcgaggt caacatcaag agttgctaaa taaaggtggt tggtatgctg
    15361 aaacgtatgc ctcacagcaa ttagaaatgg aggaagcatt tgatgaagtc taatcaatgg
    15421 caagtcttta agagattaat ctcctattta cgcccttata aatggtttac agtattagct
    15481 ctatctctct tattgttgac gactgttgtt aaaaatatta ttcctttaat tgcttcacat
    15541 tttattgatc actatctgac aaatgttaat caaacagcag ttcttatttt agtgggatat
    15601 tattcaatgt atgtcttgca gaccttaatt caatattttg ggaatctctt ttttgcgcgt
    15661 gtttcttata gtattgttag agatattcgt agagatgctt ttgctaatat ggaaaggcta
    15721 ggcatgtctt attttgatag gacaccggca ggatctattg tgtcacgtat tactaatgat
    15781 actgaagcaa tatctgatat gttttcgggt attttatcaa gttttatctc ggcgatattt
    15841 atttttacag ttactctgta cactatgttg atgctagaca ttaaactaac aggactcgtc
    15901 gctcttttgt tacctgttat ctttatatta gtgaatgtct atcggaaaaa atcagtcact
    15961 gtcattgcta aaacgagaag tttacttagt gatatcaaca gtaaattatc agaaagtatt
    16021 gaaggaattc gcattgtaca ggcttttggt caagaagagc gcttgaagac tgaatttgag
    16081 gaaattaaca aagagcatgt tgtgtatgcc aatcgttcta tggctcttga tagtctcttc
    16141 ttaagaccgg cgatgtctct tttaaaactc ctagcatatg ctgttcttat gtcttatttt
    16201 ggatttacag gagttaaagg aggtcttacg gcaggattaa tgtatgcttt tattcagtac
    16261 gttaatcgtc tatttgaccc tttaattgaa gtaacgcaaa atttttcaac cttacaaaca
    16321 tcaatggtat cagcagggcg tgtgtttgat ctgattgatg aaacaggttt tgaaccaagc
    16381 caaaaaaata cagaagcttt tgtgagagaa ggaaatatcg aatttaaaaa tgtatcattc
    16441 tcatatgatg gaaaaaaaca aatccttgat aatgtttctt ttagtgttaa aaaaggtgaa
    16501 actattgctt ttgtaggagc gacaggttct ggaaaatcat ctattattaa tgtctttatg
    16561 aggttttacg aatttcaatc tggacaagtt ttattagatg ggaaggatat tagggattac
    16621 tcacaagaac aattgcgaaa gaatattgga ctcgtcttac aagacccctt tttatatcat
    16681 ggcactatta aatctaatat caaaatgtac caagacatca ccgatcaaga ggtacaggac
    16741 gcagcagagt ttgttgatgc tgatcaattt attcagaagt tgccagataa gtacgattca
    16801 gctgtttcag aacgtgggtc aagtttttca actggacaac gccaactatt agcgtttgcc
    16861 agaactgtgg ctagtaaacc taaaatttta atcttagatg aggcgacagc aaatattgat
    16921 tcggaaacag agcagattgt acaagattcg ttagcaaaaa tgcgtcaagg tcgaacaaca
    16981 attgccattg ctcaccgctt atcaaccatt caagatgcta attgtatcta tgttcttgat
    17041 agaggaaaga taattgaaag tgggaatcac gagtctttat tagaccaaaa agggacttat
    17101 tatcgtatgt accaattaca agccggcatg atggaggtct agaaaattta ggctattcaa
    17161 aaggcataat ctgtgaggct tagaggttgg aatagttatt cctctaagcc ttttttatat
    17221 tttacaatta gtgtataatg aggtcaaagt acaaggaaag gtctataaaa tgttactatt
    17281 ggtagataat tacgattctt ttacctataa tttaaagcaa tatttaagtg tctataaaga
    17341 aatattcgtc ataaaaaatg atgatcctaa ccttttttta ttagctgaga gcgcagaagc
    17401 tatcgtctta tcacctggac caggacaccc aaaggatgct gggaaaatat tagaattaat
    17461 caatcagttt ataggacaga aacctattct aggtatctgt ttggggcatc aagcgcttgc
    17521 tgagtgcttg ggtgggcgat taaatttagc aaatcacgtt atgcatggta aacaaagttg
    17581 ggtaacaata aacgaccata ctagtttgtt taagggaatt gatagtccaa cccaagttat
    17641 gcgttatcac tcattggtag tgactgattt accagagaat attgctatta ttgcacgatc
    17701 aaatgaggat aatgaaatca tggcattcca ttgccccagc ttaaaggtat atgcgatgca
    17761 gtttcatcct gagtcaatcg ggagtattga tggaatgaaa atgattgaaa actttttgac
    17821 gttaattaat gactaaaatg tgttagtgta ggataatgac tctctataaa taaaagacag
    17881 tacctaaggg cactgtcttt tatttagttg tatagatgag ttaatctgtc tgcaaattta
    17941 cgataaataa tcaagcaaat ggatgctttt aaggtatccc caggaataaa aataaggtta
    18001 gaaaatagtg atttagttaa ggggagagaa gtttgaaagg aaagaacgat gctaccacaa
    18061 atatcaatca atagtacgcc aaatatccaa ataataagga attaaaccca gagttttgtt
    18121 gttttcactt tctcaagccc taggccgata aaaaaagctg caaatggata agtcagcaga
    18181 tagcctgctg ttggcccaaa cagcgttacg agacctgacc gccctcctgg taaaatggtg
    18241 caccaatagc aactaagagc aagaaaatag ccacagctag gaaacctttc ctactaccaa
    18301 gcagagcacc tgccagcata acaccaagat tttgtaagac aattgggact ggaatgaaac
    18361 ctaatgggat ccctgggata aaacctaaaa caatgagtag tgcaaccatg agtgcaatat
    18421 aggtagttgt tcttgttttc atttaaatta attccttttt agtatgattt agttgtaatt
    18481 tttccaagaa ttcaatatcg gtatgttgat tgcgaccccc ctctgttagg taatttccca
    18541 aaaaagttga gtcaacaagt aaggtgacta atgattcaaa attctcaaaa tggacctccc
    18601 gtccagcact taatcttaat tccttgaaag gaaaaacaaa acgcaacatg gccataattt
    18661 ttaagcattt aatgggagtc aaatagttat attttccaag aggtgttcct tcaacagcaa
    18721 gtaaaaaatt gacagggata gaataggggt tcagttcttt tagtctgaaa gctaatgtga
    18781 tgagcccctc atcgctctct cccataccac aaatgaatcc agaacaaaca tcaatgtctt
    18841 cattgtgaat tctttctaaa gtatcacaac gatctttgaa gctatgcgtc gttgcaatgt
    18901 tggggtagtt ttcttcgggg gtgttgaggt tatgattgat acggtcaatt cctgcttgtt
    18961 ttagctgatg aagttgttct ctatccaaaa aaccagcggt aaggcatatt tctagaggaa
    19021 ggcttttttt gatttcaggg atgacttgac ataactgctc aatttctctc ttgctaggct
    19081 tagttccact catcgcaata caaaagacgc tagcaccatt ttgatgagcg acaatagcct
    19141 gttttagaat gacattttga ggaagcagac caaatttatc aatgtcagca gtgctttctt
    19201 tagattgaga acagtaacca caattttctg cacaaagtcc ttgcttagca cttaataaaa
    19261 cgttaaggcg aatattattt cggctaactt gatttttaag gtataaggca gcagcgtaga
    19321 tttcccacca gttctcatcg ctatttagaa tttccagcgc ttgttcataa gaaatgctag
    19381 tctttccaga taatatttcg tcagccaaat ggatataatt tgtttgaaat gacatgatag
    19441 cctcctttaa cgaaattaat aatagcataa aaaaactgta ttgtaaactt agtctatata
    19501 ataagttaac aatataaaaa attatgatat actggtctta tattttgacg ggagataaga
    19561 tgaaaaaact atttaaaaaa gcgatagcta tgtgccgaga tttgccgctt aattttgtaa
    19621 ttgttgaact tagtttgtat caaagagagg atggctcatc tgataatatt aaatggattt
    19681 ctaagcgaat ccttgaggta acggtaactg atcagcaatg gatttaccat ttgaggatgc
    19741 ttaatttacc aaaacttaag ccaatgtcac tagcatccag tgactgtcct gcaagggaag
    19801 agaaggctag tcttatttca atagaagaat gtcaaaaatt cactagctta gtgcgagata
    19861 aaaatccttt tcaccaaggg gaagcagcta ttgtgcctgc ggctctatta ttggaaaaaa
    19921 taatggaagt tagaaatgga aaaataaagg agattcattt gactttttta tctccagtaa
    19981 gggtgaatca agaattctca gtgcaagtag tggataacac agtgattatt tttcagcatc
    20041 aaatattatg tgtgaggggg acatatgaag aatagagatg tttatattgg ttttgggcta
    20101 cgaacaccta tcggtataaa agggaagcaa tttaaacatt accgtccaga acttttagga
    20161 gcacacctct taaatcaaat aaaaaaaata gaatcagaat ctaatattga tagtattatt
    20221 tgtgggaaca cagttggtac tgggggcaat attggtcgtt tgatgactct tttttctgat
    20281 tatgaatcct atattccagt acaaacgatt gatatgcagt gtgcttcatc aagttctgcc
    20341 ttgttttttg gttatctaaa aatcagtgcc ggtattaatg aaaaagttct tgttgggggg
    20401 attgaaagta gttctcttca acctatgaga cgttacgcta aagaagataa tcgtaacgga
    20461 gaatataccg ttgctcagtt ttctcctgac tcttatgctg aaactgtaat gttagaaggg
    20521 gcacaaagag tctgtcaaaa atatggtttt agaagagaaa tgttagataa attggcattc
    20581 ttgagccata aacgcgcctt aacagctaaa caaggtggct atttagaaga ggtaatctta
    20641 ccaatggaag ggatgcgaga tcaaggcgtt agaaaactaa aagaagcatt ttttcaaaaa
    20701 ttaccaagat tgatggaaaa ttcacctttg ctcactattg gaaatgtttg tttaatgcac
    20761 gatgctgctg catttctaac gcttcagagt cagaaaacag aattccggat tgttcacatt
    20821 gtagaagtag caggagatcc caagcttagt ccagaattgg ttcacacggc tacggaaaaa
    20881 ctattaacag aaactcatac taaaatatcg gattatgatg caattgaatg gaatgaacca
    20941 tttgcagcga ttgatgcttt atttaatcat tattatcctg aagagagaga aaaattcaat
    21001 atttttggag gggcattagc ttacggacac ccttatgcct gctcaggaat tattaatatc
    21061 cttcatctta tgcaggcatt aaaatataaa aataaaccta tgggtctaac tgccattgca
    21121 ggggcaggag gagtcggaat ggccatatca atagagtact taggagttaa gaatgcttga
    21181 atcattgaaa acaatagtta aaaccaatag cgataaaaag ttatttgatg gtgatttaca
    21241 ggtcagttat ggagaatttt ataacttagt cagacaagat atggcgtcac aagacaatag
    21301 gaaacatgtg attagcacac actctctgct taaccaatta gtaagatttg tttcaaagtt
    21361 atgtcaaaaa gctcttccta ttatttgtaa acctaacctt actcataatg aaataagtcg
    21421 attagaaaaa gaagtgcagt atgctcctca actagctgat tttggtgttc tttcttctgg
    21481 gactacagct gatgctaaac tattgtggag gagcttcgct tcgtggtctg atttttttag
    21541 cattcagaat gcttattttt cggtcacttc caatagcaga ctgtttattc aaggtgattt
    21601 ttcatttact ggtaacttaa atttagcgct atccctttta ttactggggg gaacgcttgt
    21661 tgtcacacag aaaaattctg taaaatactg gcagacttta tgggaaaaaa ctggtgtaac
    21721 gcatctatat ctgctcccta gttatctcaa gttagtggaa caatatagca aggagactgc
    21781 gttagacaat aaaactatta ttacctcttc gcaatatgtc tctgactcat tactggaagg
    21841 tttatatata aagcatccta aagtgtcagt gaaaatcttc tatggtgcta gtgaactcaa
    21901 ttatgtgtca tggtacgatg gtcgtgatat ccgagataag ccacagtatg ttggagaaat
    21961 cgttccaaat gtagctgtta ggattaaaga gggaagaatt tttgtaaaaa caccgtatag
    22021 tatctgtggc ctttcaagtg aatattgtgc tggtgattat ggtgaactga tagatggcaa
    22081 attatatcta tttggtcgtg gtgacgattg gtgcaatcaa tcgggcatta agctctattt
    22141 acctagatta attgaaaaaa taaaaacatg cccttatatc aaggatgcag tagcatttac
    22201 aaaagagagt caaagtcatg gacaagagtc acattgctgt attgttttaa ttgaaaatca
    22261 gatgcaacaa gagtgtttga agtggttatc ggaacatttt gaaaagaagt atgggtttaa
    22321 gcattatcat attgtatcta aaattccttt gatgccatct ggaaaaattg attatcaaca
    22381 actcaaaaga caattagcat gagtttttac taaaaaaata aaatagggtt aatggattgc
    22441 aaacctcagt tctgatattc tttattttga aagatatcgt gatataatga taaaaaagaa
    22501 agataaggaa taaaatgtta tcaaaagcaa aatcacgata tattatccgt gagattatta
    22561 agttatttcc agatgctaaa cctagcttag actttactaa tgtctttgag cttttggtag
    22621 ctgtaatgct atcagctcaa acaactgatg cagctgttaa taaagtcact cccgcccttt
    22681 ttgaacgctt tccaaatcct ttagttttgg cacaagctga tcccaaagag attgaaccct
    22741 atatttctaa aattgggctt taccgtaata aagctcgatt cttaaatcaa tgcgctaaac
    22801 aattgataga gcattttgat gggaaagtcc cccaaactcg tcaagagcta gaaagtttgt
    22861 ctggcgttgg aaggaaaacg gctaatgtgg tcatgagtgt tggatttggt attccggctt
    22921 ttgccgtaga tactcatgtc actcggattt gtaaacacca ccagatttgc aaacagtcgg
    22981 cttctccact agaaattgag aaacgagtca tggaggtttt accgccagaa gagtggctag
    23041 ctgctcatca atccatgatt tattttggtc gtgcaatttg tcatcctaaa aatccaaaat
    23101 gcgaccagta cccacaactt tatcactttc ctgataattt aaagtgatta gaataaaaga
    23161 gagtaactgg tgacgattat ataagcgata cttaaaaacg gtagaaaagc tatcgttttt
    23221 ttcatttttc tcataactaa acaataaata attcctaacc aagcacctat ttgaataata
    23281 aaaagcattt gatgaagagg taaagataag ccaattgtgg ctaaataaag gaaatctcca
    23341 cttccgatat taatatcaac cacttcagct aaaatcccaa ataaacataa aacaatagaa
    23401 acttttgtaa tcggtgtgag aattatcgtt gaaatggtg
//
LOCUS       DATROY010000012        23140 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.012, whole genome shotgun sequence.
ACCESSION   DATROY010000012 DATROY010000000
VERSION     DATROY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23140
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(37..603)
                     /locus_tag="VBD11_000473"
     CDS             complement(37..603)
                     /locus_tag="VBD11_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6953449.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(596..1531)
                     /locus_tag="VBD11_000474"
     CDS             complement(596..1531)
                     /locus_tag="VBD11_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6953450.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1624..2265)
                     /locus_tag="VBD11_000475"
     CDS             complement(1624..2265)
                     /locus_tag="VBD11_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6953451.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2246..2830)
                     /locus_tag="VBD11_000476"
     CDS             complement(2246..2830)
                     /locus_tag="VBD11_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6953452.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2900..3045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3114..3713)
                     /locus_tag="VBD11_000477"
     CDS             complement(3114..3713)
                     /locus_tag="VBD11_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO6953453.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKIGNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3706..4377)
                     /locus_tag="VBD11_000478"
     CDS             complement(3706..4377)
                     /locus_tag="VBD11_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953454.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4367..5242)
                     /locus_tag="VBD11_000479"
     CDS             complement(4367..5242)
                     /locus_tag="VBD11_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6953455.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5280..6788)
                     /locus_tag="VBD11_000480"
     CDS             complement(5280..6788)
                     /locus_tag="VBD11_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6953456.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6829..11133)
                     /locus_tag="VBD11_000481"
     CDS             complement(6829..11133)
                     /locus_tag="VBD11_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO6953457.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDISVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBD11_000482"
     CDS             complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBD11_000482"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6953458.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11706..11978)
                     /locus_tag="VBD11_000483"
     CDS             complement(11706..11978)
                     /locus_tag="VBD11_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953459.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
     gene            complement(12260..13408)
                     /locus_tag="VBD11_000484"
     CDS             complement(12260..13408)
                     /locus_tag="VBD11_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6953460.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
     gene            complement(13398..14246)
                     /locus_tag="VBD11_000485"
     CDS             complement(13398..14246)
                     /locus_tag="VBD11_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6953461.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            complement(14243..15667)
                     /locus_tag="VBD11_000486"
     CDS             complement(14243..15667)
                     /locus_tag="VBD11_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6953462.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            complement(15667..17031)
                     /locus_tag="VBD11_000487"
     CDS             complement(15667..17031)
                     /locus_tag="VBD11_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6953463.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            complement(17033..17980)
                     /locus_tag="VBD11_000488"
     CDS             complement(17033..17980)
                     /locus_tag="VBD11_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6953464.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            complement(17989..18924)
                     /locus_tag="VBD11_000489"
     CDS             complement(17989..18924)
                     /locus_tag="VBD11_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6953465.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            complement(18926..19984)
                     /locus_tag="VBD11_000490"
     CDS             complement(18926..19984)
                     /locus_tag="VBD11_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6953466.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            complement(19984..20706)
                     /locus_tag="VBD11_000491"
     CDS             complement(19984..20706)
                     /locus_tag="VBD11_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6953467.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            complement(20706..21530)
                     /locus_tag="VBD11_000492"
     CDS             complement(20706..21530)
                     /locus_tag="VBD11_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6953468.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            complement(21555..>23140)
                     /locus_tag="VBD11_000493"
     CDS             complement(21555..>23140)
                     /locus_tag="VBD11_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6953469.1"
                     /translation="AIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDN
                     NYRFGSGIKNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGK
                     AIGNIPVGLTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEY
                     GKRITYRIPIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISV
                     DGDKIVLNSSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYN
                     LSVAQEKKGVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQN
                     RLLQGSEYILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNNIIGI
                     DGYSNDIDLNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPF
                     YFTVNKDSVSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILF
                     AVALFIFYITAIRFVFKRM"
BASE COUNT         7356 a         4425 c         3528 g         7831 t
ORIGIN      
        1 tagcaattat catttttgct aattgcctaa agatagttac acctcatttt catatgattg
       61 taacttttct accataatta aaaagggggg cgtattaact tgattgagtg gttgataaag
      121 cattgtagcg aaattattct gtggtaattg ttccacgaat gctatcaccg catctctctc
      181 actttttcca ccatcatgac cataataaac cataagtgat agacgtccac ctacttctaa
      241 aatgtctaaa acgtttttga ttgccttgat agttgtgtgt ggttttgtaa taactgtttt
      301 atccgctgag ggtaagtatc ccagattaaa tatagccgct cttaaaggtg tatgaacata
      361 ctgttccaaa ttttcatgcc catccaagat aagttcagcg ttagagatcc cttgctccgt
      421 gagctttgct ttcgtttttg ctattgcttc ttcttgaata tcaaatgcat aaactttttt
      481 agaacttttc gctaaaaaaa cagtatcatt cccatttccc atagttgcat caagcgttat
      541 tgcttcttta tcaataactt ctgctaaaaa atcatgagaa agatgaattg gtcttttaat
      601 catgttttac ctcaacttta catccttgat atgactgtct tttctccatt tctttgtcaa
      661 tggcattaag cacttcccat ttattaaggc tccacatcgg tccaattaac atatgacgtg
      721 gcgcatcacc agtaattcgg tgaataacaa tatgttttgg aataatctcc agttggtcac
      781 aaataatcga aatatagtct tcttgactta acaaacgtaa acgaccttca tggtaatctc
      841 tctgcattct tgtattaatc ataagatgca gaaggtgtaa ttttataccc tgaatatcat
      901 tatccgtaac acatcgacga acattttcca ccatcatatc atgtgtctcc cctgggagac
      961 catttattag gtgagaaacg atttctactt taggagctaa ttctcgtact ctcttaacag
     1021 tttttttgta aaggtcatat gaatgtgctc tatttattaa tgcagaagtt gcttcataag
     1081 ttgtctgaag gcctaattct aaagtcacat gcattctttc agaaagttca gcgagatagt
     1141 atatagtttc atcaggtaag caatccggcc ttgttccgat gttgatcccg ataactcctg
     1201 gctcattaat agcctgttcg taacgctctt taattatctc taacttagca tgggtattgg
     1261 taaaattttg aaaatagact aagtatttat taacctcagg ccactttcta tgcatgaaat
     1321 caatttcttt atagaattgt tcacgaatag gagcttctgg agcaactata gcatcccctg
     1381 aaccagaaac tgtgcaaaaa gtgcaccctc ctctagcaac tgttccatct ctgttaggac
     1441 agtcaaaacc agcatctata ggtaatttaa atattttttc tccaaagagt tctcgataat
     1501 aatcattaat cgcacgataa cgttttttca taggataatt gtatcacaat tttaactaaa
     1561 ataacctcac tactacaata aaactaaaaa agattggaac gtcagttagt cccaatcttt
     1621 tatttacttc actttcttta accaatcctt ggataaaaag atatacgcag ttagattcaa
     1681 aataccataa gcaagtataa aaccagctaa aacatctgtc ggaaaatgaa cccctaggta
     1741 aatacgagat aacccaatta aaaaaatgag caaacccaat gtaccttggc acaacagttt
     1801 ccatatactc ttaggcatat agtactgcaa taaaataata ctactcccaa atatcataaa
     1861 tgttcccatc gagtgcccac tgggaaacga atagccacct gcaaatacta aatgggttaa
     1921 agttggtctc actctttgaa aaataagttt taaagaaagt atacatatac cagagataat
     1981 agcatttact gcgataaatc tagcttgagg ataccacttc ttaaggtaac agaaagtgac
     2041 gctcataatc gcaatagcta tctggcttac agtattacca accacagtga ttaacttaaa
     2101 aaatcttgta gaaagatttg gcaactgtcc tctaacactt tcttgaatag tttggtcaaa
     2161 tgcgattaca gtgtcgggcc aatatttgat gaccaatcct aaactgaaaa ataagataat
     2221 agcaataaat gcttgaataa gtttactatt ttgacgagat aacattagtc tttttatatc
     2281 tttctaatat tggcaaacaa gccacgtaag ttagatagaa aacaatcgaa attaaaattc
     2341 cctcaacgat attaaatgga ataaccattg ttaaaaggta attgcctaca ccaataaatg
     2401 ttctgatatc aaagttagca aatatagcat acaaaggaat cgcaaagaca tagttgagag
     2461 ctaccataga tacagtcaag ctaactgtac caaataaact agctttaata aaatcttttg
     2521 cactctctct atttttccag aaaatagcga aacttgctaa aaatagagct agagcaacca
     2581 tattcatcgg taaaccgata aaggtttctg gaccacgatt agcaagtata acttttaaaa
     2641 gtgatcttaa taagagtaca ccataacttg atttcaaatc aaataaaata aaagcaacta
     2701 acatcggaag gattgaaaaa tcaaccttta aaaattctgc tcctggtatt aatggaaatg
     2761 aaaccatcat caatacaaaa gataaggcag aaagaatggc gattgtcacc attttacgtg
     2821 tatttgtcat aaaaaaattc ctccaattta aataaattga aagaagctcc aaaggtaagc
     2881 gtaggtacgc gaaaaaaacc tttgtcttct cccatccaga ctttactgtc ggttgtggaa
     2941 tctcaccaca tcagctttcg ctcgcggact gatgcttcac aactgacaaa taagttggaa
     3001 gcgattaccg ccggtcggga attacaccct gccctgaaga cacctatagc ataacaaaaa
     3061 aaacttgcaa ttgcaagttt tttaatcact aattagtagt agattgtata atattaattt
     3121 ttaacatcaa ttaattgaca gcgcactaat actctagcta ctcctgcctt tgtacaagta
     3181 aacaagctta agtcccaatc attgtctgat gtggcagttt tataaacttt ttcaatcgct
     3241 gttggttcaa taatttctct attaccgatt ttgtagtgat agatttgccc tgttgtagtt
     3301 gtaaaataaa catccgttcc catatctaca ttttttaaag catcaaaatg ataaggaaaa
     3361 ttatgcgcac aaatcaccat gttattagtt aaataagaac cataatacct tgtaggcgtc
     3421 ttagacagtt gttcaaaact ataattagca gctaccggta aatgcagttt taagttcgga
     3481 atatccagag ttcccaagta atctgtttta tccaactttt ttacaggtaa ttctccattt
     3541 tctgaaccct ttacttgatg cgtaatagat ttatcaagca ccttgacaat atgctgagaa
     3601 gttaaatcag cttgatgcgc ctgattaata ttataccaac cccaatagat tccagaactt
     3661 accagaataa ttccgagtat agctaaaaaa tttgctgaat atcttctaat cacgtcttct
     3721 tctccatttt aaagctatta ttaaacacag aagtcctgac ataattagta taggtattgg
     3781 ccaccatact tgtccagtaa acggaagctt tccctttgtc tgatgtgtta ctgtaggagt
     3841 aattgtctct tcttttttag gtttagcatt taaagggctc attttctcaa atgctgtaat
     3901 atcgtacttc ccatccttag gtattgatag tataaaggga gacattagtt cataaccttg
     3961 agccgtttta gtctgaataa atagataaat cccttgagga agattgttcg caacaatacc
     4021 ttcagccggt aaattatcaa acgtttgtaa aggttgagtt ttatgaacag cttttgttag
     4081 tagattgacg tatttggctt ggttactatc aaggtttact tgtgttagat catcgtcttt
     4141 tattccaata ccttgaaatg gggtagttag agtaaaaact tgattaccat caacatcttt
     4201 agcttgtgct acttggtaaa caagtaaatt accgccagcg ataccttgat tattatactt
     4261 attttgtata gtaatagaac ccgttttcat ctgatcattg gtatcagcag acacaagttg
     4321 agtacttaga ctaaataata agagaagagt tatctttagg atctttttat aaatcattgt
     4381 tctcttcctt tctcattgct tgttttaaaa ttttcttacg ttgacgtgct ctcctagtta
     4441 cttctaaaga gattaaaagt aaaatcaaag taaggaaaat agcgataaat ggtgcgatat
     4501 aaataggctc tatttgtatt gcctctgcta ctaccaaagc gttaccatta tcgtttggta
     4561 cacgatgtcc tctcactagt aaccgatggg tattaacgcc atatggtgta cacgtcacca
     4621 aagtttggta gtctttacct ttaacaattt gtaaatccct caaatcatcc ggtttaactg
     4681 ttctgatttg atccacttga taagtatatg tttcatttaa gatactgact gtccagtggt
     4741 ctccaacttt taacttatcc aaatcagaaa aaagccttga agagggtaaa cctctatgtc
     4801 ctgataaaat agaatgagtt gagtctcctc caattggaag actacttcct tctaaatgac
     4861 caatagaagt ttgaagcact ttttcacttg taccatgata aagtggtaat tttatgttta
     4921 tctttggaat tgaaatataa cccatattac ccgttttatc gatagccagt tgtgaattat
     4981 aatccaaacg ctcttggcta gtcatgtgcc acttcattcc tgaagtttta aattgcttat
     5041 tatattcttt ggctcggtta ataatttttt tataatcgtt ttcatccata tgcgttacgc
     5101 ggtcttggta atccataatc gctcgagatt ggtgaaatga attccaataa ttagcaagtg
     5161 aaggataagc cattaagcct acccccactg caattatagt gacaagcaaa atggatacta
     5221 aatgttgtct tatttttttc atatatttta aatctgtacc actttgctag cccatcttat
     5281 taagaacgta aacgacgacg agcaacaagc acgatacctg ctcctattac taaaattgca
     5341 cctataatgt agaaaattgt tgtaccaata ccacctgttg aaggcaactc agtacctttg
     5401 ttattttcaa cagttgggtt aactaaaagg ttatctgaat tagtcgtatc agtggctcca
     5461 tctcctaaaa taaccttctg agagttatct aacaaattgt aacctaaggg agcctttttc
     5521 tcaactagat agtatgtacc ttctttcaag cctgtaatgg taattatacc atctgctcct
     5581 gttgtatatt ctgttgcatt agcttctgtg ccccattcaa cgttatttgt atcgttaaag
     5641 tttagaaatt gacccgtagc attctttaaa acaaatatag caccttgtaa tgaagctttt
     5701 gtggaaccat caattttttt tatagtaatt tgaccatccc tcactgttac tttttgacct
     5761 gggtcatcat tgctagtatt ggggttgatg gtcgcaatgt ttgtattttc tggtaaatca
     5821 gctgaacctg gtttagctcc actctttaat actcctgtat aagtgactgt gattgtattt
     5881 attcccttat aaaaaaagtc atcattagct ccattttgag tatttccggt tggagtattg
     5941 gtagctgccc acggaatagt aatcgtgaaa ttattatttt cctctaacag gttatacttc
     6001 ccagttgctt tttccgaacc ttgagttaga gttgtaatat tccctgatcc atcagtaata
     6061 gttacttcat aagacccttc gttcaaatca actacagaag cagatggcat agtatccttt
     6121 ataacatatt gatacacttt ttctgtacca tgataattga ctgcattctt ataagtaata
     6181 gtatatttga ctgtatcacc aaccgagtac gttttttgat ctacagtttt tccaccacca
     6241 tctccccatg tcgcatcagt attcttttca tgaatagtag catttggagt tacagatgta
     6301 accataatta cagctccatt attaacagtg ctagaaacat aataatatcc atattgggaa
     6361 acattaataa cctcagtacc atcattattt gactcagtaa cagtggaaac tggtgtagta
     6421 ttagctgata tagatttagc ccatgtcgca atctcatttg ctgacgcagt atctttttta
     6481 gttacatatg ttctccctcc attagtagtt gtcgtaaaaa gagaattaaa atcagttgaa
     6541 gctttatact cagcttcttt accttgagga attaaataag aagctccatc tttattcgaa
     6601 tcagatacat ttgcattatc tatttctgca tcaaaaactt tatatgcttt ataggttgcg
     6661 cctttttgag tatcttgaac tgtaattgtc cctgtctcag cggcaaaagc tatcggcgta
     6721 actggtgata cagccatacc aaatgctaaa ctcgccacta acagcgattg aatcattttc
     6781 tttttcattg aaatctttct cctaaaatca tattgatgaa tgattaattc atattttttt
     6841 tcgatagtat aatattaatc ctgatggtag agctaaagct aaaccaacta ggatataaat
     6901 gtgtgttcca atacctccag tactaggcaa ttctgttcct ttactgttag taattttaaa
     6961 agtatatact gtacttccat ccactaaatt ctcttttatt ggtgtcgcat tattaccatt
     7021 ttgttcaaag gtaactcccg tagaaatcac taatactgat atatcatttt taggtagtag
     7081 gtaccctgga ggggcctttg tctctgttag gtagtatttt cctactggca aactgaggta
     7141 gttattagca tccactaata acaagccttt atcgtttgtc accagccctg aatacatagg
     7201 atgtgaagct ttattcccat tagcatctga ttcataaata tcaaaaactg cacctgctaa
     7261 aaaattatta tcattttcga cattaacttt ctgtagtcgt actttttgct tgatacgtgt
     7321 attggtaaag ctaatatcta cgtctcctga aactgtcagg gattgtaagc cggtagcatc
     7381 ataagtttta tcagcttcac cagttgctag atttttttct gtaattgact cagatacttt
     7441 aaattcatcg taggcttgtt catctattga tatagaagtt ccataaggta ctttaaattc
     7501 cttagtctga ccatctctca gcggaaaatt ctcttgttgc aacgtttcac ttggattaaa
     7561 caagaagtct ttcgtcttat cttcatctag tccaacgaca gttttactta ctctgacggt
     7621 gtattcttta ggttgccaaa cagcatataa ggtatttgtt gcatcagggt tgttatcaat
     7681 acctattgat tgacctgctg taaattccac acgtcctgta tcagctaaat ccttatcatg
     7741 atgccaacca ataaggttgt aacctgtcct tgtaaagtat tggttttcag gaattgtagt
     7801 tgtgctattc aactccatac gcggtgtctc cacttgtgtt accacattac cattttctac
     7861 tctagtacca ccgttaccat tgtatttgat ggaggtatct tctaatttga tatctcctac
     7921 tggaataatg acaggtttta tggtgatatt tttattagca tctgctaaat gggcgtcaat
     7981 atcaatggaa tcatatgggt tataaatttt accattgtac caccagccac ggaaacgata
     8041 gccatctggc attgtcggac gtctcagtaa ggctgaatgg gagccatcgt cataggaatc
     8101 agggtcagta ggctcattac ttgtttgtaa ttgctgacca gaagcatcca atgctggctt
     8161 tccatctgta ccaacagcat cattgctgta tataatatga taatctccag cctttcgcca
     8221 aatagctctt aaattgatat cttgagttac agcacctgaa aagttataag gtctaatact
     8281 accatctgga ttaacataat accaaccgac taatttgtaa gcgtctttta cgtacttgta
     8341 cttagttgtt gtatcaacat ttgagagact agtatctgtc gtataatagg catctttagt
     8401 tgagtcggga tctttatctc gtgaatcata cttataataa tatgtacctg aaggatcttg
     8461 gatataatcc cttgtaatat ctgtataatc cggaatacga tcaccataat gcaaatctaa
     8521 ataggtatca tctgtttttg ataatcggcc tccgtttgga tcaatattga cacgatatgt
     8581 taccttttgc caacctgcat agactttaac atcatgagga ggcatagtcg tgttaaagtc
     8641 aaatacttgt gtttgtgctt ggtctttata ccatttacca tcccaaacat accctggtcg
     8701 actaggttta ggttgaacag ttgtcgtatc gggggcataa gaggacaaat tttgctcata
     8761 tagaacatcc tttactggaa aattaggaag ctctgtatta tcaagcggat ctaaatattt
     8821 aatcttgtat gaattacgtt cataccatac cactaagttc aaataatctt tgtggtaggc
     8881 accatatcta tcatagtatt catctgaaat aggaactttt actcctgcac tcgtttgact
     8941 tgcgtactga tcaaaaaggt aattatcagg atataaacgt tgataatatt taacattaaa
     9001 ccctagatat ttttctgtaa aggtaaattc atctgcccca gcacctccac cggtatctgc
     9061 taaagaataa gtgccatcca aaccttgttt gaagaaccga taagtttgaa ctatattccc
     9121 ttttggaaag agtttgataa catctgaatc aacggtatca ttaggtaaaa tgaactcacc
     9181 caaataactc atcccatagg ttccaacttg gttatttcca acattggcta aataacccca
     9241 tgcacccgtc ttccattgat aaccattagc ggccaaggtt gtaccgtaaa gtcctgtgta
     9301 agtatcagca tcagagctcc aattatacga atagtaatat tctggcaagg aatatccgcc
     9361 atattttgca aattttattg tgatgagttt tctatcataa taaacattaa caacacttga
     9421 accatcgtct tttatcatga cagaagtttc tgtcctcgta ttattaactt taaagccagt
     9481 cggtagttta tcattaatat cttgttgtgt tagcgtctga ttagataaag ataggcctga
     9541 tcgtgttact tgccctgcgt actcatatgt cttttgcgca tcagtagcat ttttattatc
     9601 cgttgctgat tgttgccagt agtttatcgt gtaggttgtt tgtgcgggcg accaatgtgc
     9661 atatagcgtc gtatccttgg tcaagacttg attaaaatca aaggctgccc caccactagc
     9721 agctgtgtac caccctgcaa aagtataacc tggcctcgtt ggatcattag gcttaattgt
     9781 cgaagcaggt tggcctgtta acacacgacg aggtgcaata taggtaactc ctgttgataa
     9841 gtcgcctgtg ttgaattcaa caccgtacga ttctttaaag acaggataaa gacttatgtc
     9901 tgaagataca ggtaatgctt gaatttctga ttcagaaagt ggagctccat tttgagtttt
     9961 agaccaacct acaaataaca atgttgaagt gggtggtgaa actttaaatg aacttaaatc
    10021 cgcggttccg tttcctgaac tatccaatgg tacttgataa cgctccaaaa tacttttacc
    10081 agcagaatca tcatataaag ttactgtcgc cactttactc ataaatggac gaacacaaat
    10141 ttcttttgtc tcagttacag ttattggctc accaaattta acagggtcac catactttcc
    10201 agtagtagga tcataggtat accaaccatt aaaatgctct cctgctttaa tcgtcggaat
    10261 cccaacttct cctagagatt ctccatcttt tataatttga tgatgaactt gcatacctga
    10321 agctgtcagg aaatcataat cagttccgtc attattttga aaatggtaag ttaacctagg
    10381 aacttctgta ttatcctctt gaacaattgc ataaatggag aatgaatctg ttttaaaagc
    10441 aacatcactg ctagtgttct tagtttctgc agtatcttta gattttaata cttctgtttg
    10501 accatcatct ttaaagtgaa caactttaag gttttcatct gaagcttcta atggcttatc
    10561 atagttaacc tctactttta ctggagcttg gggttctgct tctttaccat ttgactcaat
    10621 agtaatgtca tagagtttga aatttttgat ttcactatct tgtttagcaa cctctgtcaa
    10681 tgcttttttc ttataatctt taaaagtagc tgaattgtct tttaattccg tcacctttaa
    10741 atcagcattt ttaggaatct tagcttcttt ggtcaaagtc actgttacag tatagtctgc
    10801 acctctaaac atcaatggtt cttcacgata agcagcttcc tcagaagatg atgtttctgt
    10861 tacactagaa gcaggagtct ggggcttgct ctgctcaaca cttgattgag aactagatgt
    10921 tgatgaagtt acctggctag aatttttatt ttctaaagta atccccacat catctgtctt
    10981 agtttcttca actgttattg ctggtagaat taaaaaataa gtcgttaaaa aagttgttag
    11041 gatcatcaat gaccacatga taattttcca ctctttaggg tgttttttct tttttaatga
    11101 ttcgattata aaagtttgac acttctttag catttttgca tcctccctaa ccttaattga
    11161 tactactaat cttacctaga ggccatattc tgaaagaaat tttacctaca atttgttctt
    11221 ctgaaacatc tcctacagaa gtacttcgag aatcaattga agtttttcgg ttgtctccta
    11281 atacaaaaat ttttttatca ggtacttgat atgggtattt tatattacta ttaccgagtg
    11341 ctttatgaat aacatatggt tctttcaact tatgttgatt cacgtaaaca tccccttgag
    11401 aatcaatatt aacccagtct cctgactctg caataacccg cttgactagg accttattat
    11461 tgtagtaaaa cgcgacaacg tctccagttt taaaatttga accttttact gtaaagacta
    11521 catcacctgc acttaaagtc ttattcattg aatgtccgta gattcttaat acaggcaacc
    11581 ataaaaccgc aattaaaatg gctattgagg caaccgccat caagatgtat atggtatttt
    11641 taatgacact ccaaaagcgt ttctgataag ttacgcgatc cagttcttga gataatttat
    11701 ctgaactaat ctgtcttttc attgtctacg cctctcttat ctactaaatt ctttacgttt
    11761 tctaaatagg tatctgcagc taactgagca gtctcaaaaa taccactaag cattaaggaa
    11821 gcttcggcaa tagaaccagc tttgctaatt ttgatttttt tatcatctag cgcttcttta
    11881 agttgctgaa tctctttctc ttgtttttca ataagaagtt gctgttctaa cataatttct
    11941 agcaagtctt ttctttttaa tttttttaaa tcttccatcg cgattacttc cttaactgaa
    12001 ccttaaatta tcgtttagat attatatcaa agttctaacc tttaaactca ttttttgtcc
    12061 tgtgtttttt ctcaaaaaag tctatgctaa attaacattt ttgataattt tttgaaaaat
    12121 ctcatcgaag tcattttctt tttgaaagct cgaattctag gcattaaaaa gccatatatc
    12181 aaattgatat atggcttttt ttattattta aaacaaaaga atcaatagga caatagcgtc
    12241 aatttagtga cataatctat tacagattaa gttctttttg aataatataa tccaactttt
    12301 caactgtttt ttcccatgtg aaatgttctt taattctttt agcaatattc tgttgtagtt
    12361 tctctcttaa tgccttatct tttactaata aatcaagaga ttcatggagt gactgagtat
    12421 tttcttccat gatgattcct aactcagggc tatcaataac ttcaactgtt ccaccgcgat
    12481 ctgttgcaat aatagcactc gaaagtagac cagcttctaa aatagaggtt ggtaatccct
    12541 ctggatacat tgatgggtaa acaaagatat ctgtctgtgc cattaaagac atagtctgtt
    12601 caaagtttaa tttccccaaa aagttaatct gtttggactg atatttctct ttcaaatgtg
    12661 ctaattcagg tccgtctcct gcaatctgta aataaacatt ttcagagtac tgtgacatcg
    12721 aaaatgcttc taagagcaat tcaatgcctt tttctttaat aattctacca gcataagtga
    12781 tgaaaatatc atcagcagat ttttcaaggt aagccgtgtc agcaaaatca gaacctagac
    12841 tttcagatac cgaattataa ataactcctt tagcttctat attaaaatgt tttaaccact
    12901 caacgcttct cttagatacc gcataaaaat ctggacgata gtgcttaaca cgcgctgtga
    12961 gaagatgttc atagatagct ccaaagaaat ctaaaaaacg attattgaca gaaaaatgac
    13021 ttgacccatg gtctaaaaca atactaggta aacggtgctt ctttgcaaaa gatagccctt
    13081 ctagcgttgt taactgaaaa cgtgtattac aaatcacaaa atcaatattt tcatctgaaa
    13141 catgtttcat cagcgtgttg tattctcgat ttttgttaat aataggatag cgctgcttga
    13201 caatattttt ggtcggtaaa cggtaaattt ttctaccctt gtcttcatct ataatcggta
    13261 aatcatcatg attagttgtt acaataacaa cacggtagcc acgcttaacc aaatctgctg
    13321 tcattttatc tgtataacgt tcaatacctc cgaggaaggg tagataatat cctgagaaaa
    13381 cagcaactgt ttttacctta ttttccatat ttatccactt tcatcaataa gccatctttt
    13441 aagcctttaa tcatagcaac taattttttg ctcttttgct cttctgctac caacactcga
    13501 acaaattcat ttcgcataaa tactaaatat ttgtgccgct tcttcttacc atattttttt
    13561 ataatataaa tcgcattgcg tatcatgtaa tattttcgaa atggtgaatg attcaataca
    13621 tgaaaaacat ggccaaattt tttaactcgt gaagagtgtc caatttcgtg taaaagacca
    13681 ataaaattaa cctgataagt cttatatccc atctctgaca gacgataatt catttcagag
    13741 tcaacaaaat caataaacat ctcttctgca aagccagatg tttcttcaaa aacgctcgtt
    13801 ttcattaaag cagccgaagt aatacactct tcaatttctt tatagtcaaa ttcttgcata
    13861 actaaatctt cacggttcat atcttgatac aaacaagata acataccgac cttaggtaaa
    13921 agaaggtaat tttcataatt atctatcaaa tcacctagga caaccgaatc ttgatctaaa
    13981 gtcaagaacc aatcaaattc ttgtgctact gcaaattgac cgatacagtt caaagcatat
    14041 gcaattcctt tattttctgt taaataatca acagttaggt gcccctcttc attataatcg
    14101 gccactaatt gagaaatttc ttccttattt ttcgagccat tatctacgat gtagatatgg
    14161 cttacttgag gataaattgc tcgaatgttc tgatctaagc gttcaatatt ggggttaaag
    14221 gtgacaatac ccgctaaata tttcatgttc tatgctcttt tctaaaatct ctaaataact
    14281 gaatgactgg tgctttggtt ataaaaacga taccgacata gatagttact gctactaaac
    14341 tttgaatgac ataatttacc aatgatactg acatttgagt attgatataa taaagtacag
    14401 ctccactaag agtagcagca attaaatagc gcagcattcc tcttgttaat tctttaaaag
    14461 taaatacatc tcttaaagag atagcttgat agagggagac aataaattca gtaataactg
    14521 tagagataat agctcccata gcacctaaaa ttggtattaa aagtatatta agcacaacat
    14581 ttgccacaag tccaataact gcagacattg tgtaagcttt tgtacgtctt gaagccagta
    14641 gatactgtgt ccctaaagcg ttaccataag aaatgcaaat gatcatcaaa gactcaacag
    14701 ccattagcgg accgacagct tcataccctt tcccaaaaaa gaagacggca aatgtcgacg
    14761 aaactcctac cactcccgca atcattaaca ttgagatagc attgcttaaa tcaacaagtt
    14821 tcaaaagtaa agccttagct tgcttttctt ttcctgaaga aaatagactg gagagccttg
    14881 gtaagaaaac tcctccgatt gctgaaacaa tggtaaataa aatacgaata attttatcag
    14941 attgatcaaa atatccagca ctgaccacac tatccattgc tcccagcata ctcttgttaa
    15001 gagagagata aatctgcatg gtgatttggg gtaaaaataa cattaaagtt ggtcttagat
    15061 gtattaagat atctaatcta ctaaatggta tcttgatgat ttcatgcttt aaatgatgcc
    15121 agactgttaa atttccgagt aatgatgcaa atgcaattaa aaacatataa agtgctaggt
    15181 catctggcgt cttcacaact aagaaggtca aaacaactgt aataagctta acaatggtat
    15241 tacgaatgac aattatctta aagtcttcaa ctccaataaa ataccacgaa atatcagttg
    15301 cagtcgctaa taaattaata ccttgaatta aatagaataa ttgccattcg tttgtaagta
    15361 agacaaatcc aaagaataat aacgttgcta tagatacaga ggcaagtttt aatacaacta
    15421 attcccaaaa tatcttactt ctcttatact tatttgactg cacaaaagct atctcacgat
    15481 taccgtacaa agatatccct aaagcaccaa ataaagtaaa gtacatgaca atggagctcg
    15541 tataggcatt aataccgatg ccttgaggag atagtacacg ggaaacataa ggaactgtta
    15601 ctaaaggtaa caacaaagtc aataactggt atgaggtatt ataaaacata tttttcaaaa
    15661 gtttcattag tttctcctca taattaaact aataacagta tcaaataaca ataaggcatt
    15721 tgatacaatt accaagccag ttaacgcata cggcaccact ttccttcctg cctcatttag
    15781 acgcgctgga atacttgatg caaaaacttg taccaaaagc aatgtaacag taaagtaacg
    15841 tccttgaact ccttcaatgt agcttgttgg cgctccacga tattctaaca tgataaaaaa
    15901 gacaccaaga attcccacaa agaaactagc taagaaaaga aatcccatat tttttgactt
    15961 aacaaatttt tcttcagaaa accatactag aaagaccatg agataccata ctgatgatac
    16021 ccaagtggga ataaaattct gacttggata tggagaggca agccaggaag taatgtcatt
    16081 acttaaaata ttgataccag cataattata gccaaatgtc attacataac gtcttaaaac
    16141 agcaaggaca ccaccatgtc ttgcggtcag aacataaact atagcaagtg acaaaaaggt
    16201 tagacatagc caaaatagct ttttagattt aagaccttga aataaatgtg atagcttctg
    16261 attatagccc attaatttag gattgttaac ctgcaataat accggtatta ctagcaataa
    16321 aaacagccag taattctgct tacatgcaac aatgactaaa agaatcaatc ctataagagc
    16381 aatactacga tttttagtca acgatttatc aactaaaaga tttatcaaaa gcgataaaaa
    16441 tgctccaaat acaatatatc cagtcgagtc ataagataga ctagcaaatg aatttagaac
    16501 tacgggactg aggaaaatag caaagtagat taactttcct tttttcacat accggataat
    16561 gagggcaacc aaggtgattg agacaaagga actaaataac cttgctactg taatcattac
    16621 ccccatagaa ggatacacaa atttgcctaa aaataagcca actgctggca caagatgacc
    16681 ccaaaaaaca aagttatgtt tagaatagtt tatctcacga ggcaaatgat taccatcaac
    16741 aaaattggct ttatttaaat agtatttttc aaatctagta ccatctctat aggaggtcga
    16801 ttgtcgtctc atacctgaat caatattata ttctccatag taagaagtat caataatgtt
    16861 atttgaaata cgaccagaca cagctaaatg gtattgtcca tcaggttcat tgaaaaatgg
    16921 cattaatagg ctcaatacaa tagtaatcgt taagcctaat ataaaatatg ttttatgcaa
    16981 gttcttgtct agaaaaaact tttcaaatag acgaactttt ttgaggttca tattaactcc
    17041 tttttatatc ttttaataaa aacaccaagt cacgaatgcg atttgaaaag ctatcataga
    17101 accgtttttt agtcatcatt tgtttaagta ttgctacaaa atctttattt tgagcatttt
    17161 tataacgttg ctgattaaaa tccagataag ctttagcttc ccttaattga ctagagctac
    17221 ttttttctaa aaatggtaca aggatgatat agtaactcaa aacaaaatac agttcttgta
    17281 aatgcctttc gtaacttgag ctcatattca agatattccc acttgcattc tcagcatgtc
    17341 gcttatgttc accaacactt ctgtttaaat tgtaacctga accaatgaca ccagataccc
    17401 ctgcaaaaag attatcatgt gacaaaatta aattatcact gtaaaaagtc attacttgat
    17461 caacaaacga cttgcgtaaa caaaaagtcc aaccttgacg atagttatga tatgaaaagt
    17521 ctaatggata cttagaaatt gtttcctcac cattgaactt aaaattattg ggaatagtag
    17581 cctcttctga taactttttg atatcaatat cggcgctaag aaggtttata tcttggcgtt
    17641 cttccataat atcaacttgc atcttatttt tatgatgata ccatgtgtca tcttgatcac
    17701 taaaaaagac atagtcaacc tcataggcta acacatcaat aagtaattga cgaaaattta
    17761 aacgccaacc taaattttta tcattactag ttattgacca accatctaaa cggtgctctt
    17821 ttatatagtc ttcaaccacc tttacagtat catcagtaga gccatcgtcc ctcattaaga
    17881 catagtctgg tctcaacgtt tgatttctaa ttgaatctag ctgatcgtgg atgaaacctt
    17941 gaccattata tgttgccatc aaaactgctg tttttaccat attattcctt actttttaaa
    18001 cgcaaataat aaatctctca tacgtccact attagagtta tagagtcgcc aatttttaat
    18061 cacaaaagca ctattatgaa ttaatgacaa tttagaagct tccaatcttt ctttttcaaa
    18121 ggctaagcgt tgatttaaag cgacatcatt ttgatatttg ttgacaacct tctgcatggt
    18181 ttcgtaaaaa tcaacaaatg tttttaatcc tgtgatatgt tgtgttttag gcgacttcca
    18241 attcaaaaca aatttgctat taccacttgc gtttcctcca tatcgaatat gaactcctac
    18301 gctttcgtta aggttataac catttccaat aatactggca gttgtctcga taagattatc
    18361 gtgcgaaata ttagctccaa aagtccaatt atcagcgata gcttgcgcaa aatctctctt
    18421 catagcaatg gtccatccgg gacggaatgt ttgataatca tatactttag gatactgact
    18481 gattctatca ctagaagaaa aagttagaaa atgtggaaca ctggcttctg tagacatcgt
    18541 tttgatatca acgtctgcac tcaaaacctc aatttgaggg ttatctgaca taatggcaaa
    18601 ctgtcgttcg tttttatcaa gataccaaat atcatcttga tcactaaaaa agacatagtc
    18661 aacctcatag gctaacacat caataagtaa ttgacgaaaa tttaaacgcc agcctaagtt
    18721 tttgtcgttt ttaacaattt tccagccttc taactcatgt tttgcgatat agttattgac
    18781 gacattgact gtttcatccg ttgaacaatc atccctcaat aatacataat ctggttttaa
    18841 tgtctgttgg cgaattgaat caagttgttc agatataaat ttttcgccat tgtatgtcgc
    18901 cattaaaact gctgtcttta ccatattact cctcaaatgc ttgtactgtt tttaatattc
    18961 cagattcaag agaaacttct cgcttccatc ccaacgcttc taatttggcc atagtgagac
    19021 gtgttctccc cattttgcta taacctgcac tcatagtttt aggaatatcg aatactactg
    19081 aaatattttt atcagaaaag agatcaacta acttttgtgc caaatcctta atcataatgg
    19141 gttgatcttc atttgctaca ttgtaagctt gaccaacttc accatttaat aaaatagtaa
    19201 ataaccctga tacagcatct gctaaataac agaaagcacg ctctgctgta ccactacttt
    19261 ttaaaacgat atctttgcca tcaatgacat tcgataaaag atcattcata atacgaccat
    19321 cgttaccaag ctccatacca ggaccaaatg aatgtgcaat acgagcaata gtaaagggta
    19381 ccttatattg atcatagtaa ctttgcagta gagtttcagc catacgtttg ctttctgggt
    19441 aacaagctct agtagctaaa atatcaaatc caccataagc ttcttcatca atcacttctt
    19501 taatagatgt tccatatact tcacgcgttg ataagaacaa gaagttttca attgtttttt
    19561 cctttgcaaa gtctaataaa ttcaatgtac caattgtatt agcttttatg atgctaacag
    19621 gattactcaa aatagcagtt ggactagcat tgcttgctgc atggataatg taatccactt
    19681 ttccatcata ctctaggcgt tcttctacat cataaggaat taaggtgaaa taatctttcc
    19741 caactaaatc tgaaaattta tcacgggctt tttcaatgtt tctagctgtc gcaataattt
    19801 gcgtttggta atttcttgtc tcattgagat aggctagtaa aaataccata taactcgcta
    19861 acatactatt agaaccactt attaaaacgc ctttattagc aattctttcc caattaatat
    19921 aattttggtt actaataagt gctaaatctt cttgtacaat tttattacta aatttaatca
    19981 tcattacatc ccataaattt gttcattttc tctagcatca tacaaagctt taaacatata
    20041 aaaatcatct ggtgttgtta tcttgatatt ttcatatggt ccctcaacaa tagtgagttc
    20101 acgattgtac atacccatca aagtactaga atcaattgca tcagtaatcc ctttgctaat
    20161 ggcatcacgt tcaacagaaa ggatatctga tagataaaaa ctttgtggcg ctttagcaat
    20221 gaaggaacga gtacgatcaa caacttcaga tattttagaa gaatcattga ctagaacaac
    20281 tgtctcttta gcacgaactg aagttaccgc acttcctgtt tcttttactg actgtatatt
    20341 agcattaata acttcttcat taatcaatgg acgtactccg tcatgaatta aaacaacggc
    20401 atcatctgta gccagctgtt cagcggcttc aagcccctta aaaattgaca tttggccagt
    20461 ttctccacct gcaacaactg cttttacttt cgtaaggttg aaacgctcta ccagattatt
    20521 catataatct agccagtctg atacacagac aactactaca gcatcaatag cttctgtatt
    20581 ttggaaaata tcaattgtgt gtacaataat gggtttacca tgcacttcta ggaattgctt
    20641 tggtttacct ttggtattca tgcgacgacc aacaccacct gcaaaaataa ctccaatatt
    20701 catcattaac cttctttcca gtaccaagtg taataactat gactttctct cttactttct
    20761 ggaggtaatt ccatataact acccccatat aattgattca gataagcatg ctcattttta
    20821 atttttcggg ttttaatgtt ttcaaacata acatcttcat actcagaaaa aatttctctt
    20881 ggaaaatatt ctttcaagcg atatcttgaa tcggtataac ccatgtaagg aacctcttga
    20941 ttattatatg tttccatcac ttgatcaaca atttcagcac gttttttagc ttggccgtga
    21001 taagcgatga atcggggtag ccccaaaatt agccttgcat ttgcataaag ctttcgggaa
    21061 ccgctagcaa aattggggta agtcgtacac atcaaatttg cagcagcaga gtgaactttt
    21121 ttaaagaatc tttgacgttc ttctgcactc tctggtaatc ggtcaagtgg gaagatatca
    21181 ataaaaatcc ctgtccaagg atgaattcta tctgttttac ttaagtacat tgactttgtg
    21241 tgatacagct tggcaaatgg ccatagattt ttttcaacag aatgatgtag caatttaaaa
    21301 tcgctattat tagcatcagc taatgctttc atcaaacgct catactggct tctttctagc
    21361 atcaaatcaa tatcgtcatc ccatggaatg aatcctttat gtctcattgc tcccagtagg
    21421 ctgcctccgc ctaaagaata ttcaatttta ttatcacgcg caactttatc aatataagct
    21481 aacatttcaa gttgaacttc tcttatttct gagaccgtca tttctttcat aattacctct
    21541 gtctaacagt taactcacat tcttttaaat acaaatctta tagctgtaat atagaaaata
    21601 aataaagcta ctgcaaaaag aatcactagg gtcatgggtt tgaaagtttt ctttgttttt
    21661 tgaccttttg gagtgtttgc aagtgcattg tattcttgtt tgttgtactg catgtccatt
    21721 atataaccat ttatattata gttaccaaat tgtgctttag aaacactatc tttatttact
    21781 gtaaaataaa atggtttatt gctttgtaag tagccttttg gagattttgt ctgtattaag
    21841 aaatacttat acttattaga caaacctcgt aatttaaata atgattggtt acttttcact
    21901 tgttttttat agttataagc ccataaccta ggattcaagt ctatgtcatt tgaatagcca
    21961 tcaataccta taatgttatt tcctctaata tggtaagcat tttggttaag atattcttta
    22021 tttaaactct taatcgtttt ttgagtattt atccaaaagc cattaggttg atatagagat
    22081 acttttttat ctgcattcaa tcttcctaaa atatactctg aaccttgcaa taatctattt
    22141 tgattgtcgt catacatggc aaaatttata ccataactgc tcaaactagg tgtctttata
    22201 tagcctttag aatcctcgac aaaaatccct tgatcttgtt tttgattatc aacagaaatt
    22261 agactccgtt tagtctctat gacgcctttc ttttcctgag caacagacaa attataatct
    22321 ctttttgaag caacatgtcc tgtaatctct aactctttaa tatcagcatt tgtcaaatca
    22381 aagtctaact tatcaataga gtgaagttta tacaggctat tttgtaataa ttctttttgc
    22441 tctgtttcat caccactact atttaaaaca attttatcac catcgacact aatactgcta
    22501 tctattggat acaaacgtcc ctctttacct tcttccgcaa taataggcgc ttgagaaagt
    22561 cggtaattat aattagtact atctactaca aaattagggc taactctgac agtcagctgc
    22621 tttgaataaa ttggaatcct ataagtaata cgtttaccgt attcaactcc tatagtatct
    22681 ctttttttgt tattaacagt tactcgagga ttatctactc gggtattacc aagataaatc
    22741 gtttcgtttt tctgaatatt tttcaacaca tattcttgag tttctgcgct gttttcaaaa
    22801 gcagttaaac caactggaat attaccaatt gcttttcctt tgttatttgt gaacttataa
    22861 cgtttatttt ctggattata taaaacactt ggctcttcta agccattcat gtaaattaaa
    22921 taatctttat tcttttcaag cagtaacctt acaatagcaa tagcatcctg tctagtattt
    22981 tttataccag aaccaaatct atagttatta tccataagga cttttttacc actaacttgg
    23041 tacttccgct tgatactatc aaaagtatag tgtgtaaagt ctttgctctc aaactccccc
    23101 gtcctcactt tatccaatgc tgtttgtaaa tgaatagcat
//
LOCUS       DATROY010000013        21131 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.013, whole genome shotgun sequence.
ACCESSION   DATROY010000013 DATROY010000000
VERSION     DATROY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21131
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..275
                     /locus_tag="VBD11_000494"
     CDS             <1..275
                     /locus_tag="VBD11_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953470.1"
                     /translation="ESYAKANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSM
                     TSTYAKVFRQKDAKSRLVYEGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            363..632
                     /gene="rpsO"
                     /locus_tag="VBD11_000495"
     CDS             363..632
                     /gene="rpsO"
                     /locus_tag="VBD11_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6953471.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            1012..3141
                     /gene="pnp"
                     /locus_tag="VBD11_000496"
     CDS             1012..3141
                     /gene="pnp"
                     /locus_tag="VBD11_000496"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6953472.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            3143..3895
                     /locus_tag="VBD11_000497"
     CDS             3143..3895
                     /locus_tag="VBD11_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6953473.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            3904..4488
                     /gene="cysE"
                     /locus_tag="VBD11_000498"
     CDS             3904..4488
                     /gene="cysE"
                     /locus_tag="VBD11_000498"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6953474.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            4498..4680
                     /locus_tag="VBD11_000499"
     CDS             4498..4680
                     /locus_tag="VBD11_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953475.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            4677..6020
                     /locus_tag="VBD11_000500"
     CDS             4677..6020
                     /locus_tag="VBD11_000500"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6953476.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            6013..6399
                     /locus_tag="VBD11_000501"
     CDS             6013..6399
                     /locus_tag="VBD11_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6953477.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            6502..7257
                     /gene="rlmB"
                     /locus_tag="VBD11_000502"
     CDS             6502..7257
                     /gene="rlmB"
                     /locus_tag="VBD11_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6953478.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            7254..7772
                     /locus_tag="VBD11_000503"
     CDS             7254..7772
                     /locus_tag="VBD11_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6953479.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            7865..8725
                     /locus_tag="VBD11_000504"
     CDS             7865..8725
                     /locus_tag="VBD11_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6953480.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            9262..9384
                     /locus_tag="VBD11_000505"
     CDS             9262..9384
                     /locus_tag="VBD11_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6953481.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            9606..10052
                     /gene="rplM"
                     /locus_tag="VBD11_000506"
     CDS             9606..10052
                     /gene="rplM"
                     /locus_tag="VBD11_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6953482.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            10073..10465
                     /gene="rpsI"
                     /locus_tag="VBD11_000507"
     CDS             10073..10465
                     /gene="rpsI"
                     /locus_tag="VBD11_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6953483.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10599..11753)
                     /locus_tag="VBD11_000508"
     CDS             complement(10599..11753)
                     /locus_tag="VBD11_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6953484.1"
                     /translation="MSVHKYQSTKGITYFVKVYLGLNDYGKKKYYTKRGFKTRKAAKA
                     HETAVNHQLNSGTFVHLTAQTTYTYQELYQRWYEAYKDTVEATTASKTADLYRLHILP
                     VFGEKKISKISPLDCQTFITNKAKTFKNMKQIKSYTSKIFEFAINMNYIERNPMSKVI
                     MPKFKKTVSENYWTVNELHQFLTIILENEPYKHYALFRLLAYSGLRKGELYALKWEDF
                     DSDSQLLTVSKSLGRIDGHAIEKETKNTFSVRSIYLDDETCSILNKWRQETCKEKGQL
                     SVTPLSIDKEFMFTYCNRDGEIEPLHADYINNILKRIIRKHHLKKISPHGFRHTHATL
                     MIEMGIDPVNTAKRLGHASSQMTLDTYSHATKSGEKQSIKKFADYLNKAK"
     gene            complement(11807..12325)
                     /locus_tag="VBD11_000509"
     CDS             complement(11807..12325)
                     /locus_tag="VBD11_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6953485.1"
                     /translation="MTNHIAKLIEESGKKLTEISSATNIAYPTLSGYNQGIRQPKKGN
                     AQKLADYFGVSVAYILGLDDNRQAPSTMDIITDSFKVSEISSVTPFKSDMEKLKKGIE
                     LGEIHLSMPLNEGFSDDFRRILARYLVNHEEEFMKTFTKHMNSLKRESEIWQTWIQTE
                     EYRIRQEDRHKK"
     gene            12473..12757
                     /locus_tag="VBD11_000510"
     CDS             12473..12757
                     /locus_tag="VBD11_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6953486.1"
                     /translation="MTDETTLVVQLPKSLERQLRAYYDGMVSSAIERALEDKELYKPM
                     VRMSALSRWLDVSTTTIQKWTREGMPTMVIDGVTLYDKRAVTRWLKQYER"
     gene            12766..13251
                     /locus_tag="VBD11_000511"
                     /pseudo
     CDS             12766..13251
                     /locus_tag="VBD11_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
     gene            13303..13590
                     /locus_tag="VBD11_000512"
     CDS             13303..13590
                     /locus_tag="VBD11_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6953487.1"
                     /translation="METLWHRELTQQEKTLLEGLTVNQSLLQLAIQKTAELEPRKQNM
                     SYVKGILTNWEARGLITADQVIASEKDYHPLEISNVEVSEDFLAAMDLWKD"
     gene            13605..13895
                     /locus_tag="VBD11_000513"
     CDS             13605..13895
                     /locus_tag="VBD11_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953488.1"
                     /translation="MTVYYQLENMLVAGFKSQEEYNCYQELKRDYESETEDYSFSIRE
                     ITNQLEIICQTSENDFPDLEEGILTDYLELVEQLKRYDTKLADRYLRMIHHV"
     gene            13888..14250
                     /locus_tag="VBD11_000514"
     CDS             13888..14250
                     /locus_tag="VBD11_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6953489.1"
                     /translation="MSERVRNVLKKFYVTESQNKVLNQLVDDTGLQTFSNYARRMLLK
                     ETSLFMRFDESQFDELIYSLRRIENNLRQLSKIAEQSQDSQAYRAMEYSRRLVSNYEK
                     QLTRYHQKKKRKLLSKGA"
     gene            14250..14606
                     /locus_tag="VBD11_000515"
     CDS             14250..14606
                     /locus_tag="VBD11_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6953490.1"
                     /translation="MAKKRIRNVIKQVFLSEEENQKLLERMKQDGISNFSLFARKQLL
                     KPDFEAWVVSFPEYQSLTDRLLSIGRTINSIAKSATQFGKISRQDLMELGQLMEEHVE
                     LVEKQLKEDKQRIAKK"
     gene            14952..15644
                     /locus_tag="VBD11_000516"
     CDS             14952..15644
                     /locus_tag="VBD11_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HEO6953491.1"
                     /translation="MKENLIKSKHRVQKHGEVFTPSWMVQKMLDTPGVKEACENISAT
                     FLEPSAGDGNFLQAILERKLASVIKQYDKRNWKTKSLIALSSIYGIEFLEDNLEVARS
                     RMFLYYLDWYEITFGDRLTNKSDIYKSAYYLIHKNIVRGNTLTQRHPVTGDLIWFNEW
                     QVVKGHPSLVRKIPFSLSELLGKEVENDKSIVEGQLSFFDIDDNFVVEETAEKILESK
                     EINIQKVYLLGD"
     gene            15647..20050
                     /locus_tag="VBD11_000517"
     CDS             15647..20050
                     /locus_tag="VBD11_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEO6953492.1"
                     /translation="MISNFEFLAIDIDTSELFKTINMAEENYTHGDYEGTLTKVRKVA
                     ENTARLIADREYIDIRERATFNDVLKDIRGLIPNKKIVDNFYEIKGKGNNSAHILNPE
                     DATKENALKSLEQVFDILAWVMLTYIDPDIKASAIGRFLEPRAQEMYKTAERKFIYIQ
                     TVDNQSGLFNAFEGTQKIGEGTVSSDDIETDWSPNSEFLRTAAPKRINQYMKTSGLPY
                     TLGWVELAYKKSTKSWFHDYDVHNVLKRSGVKQAEQLEGTEWFKTDLETAKSAIKAVK
                     EGKNYLNIPADETVVEEPIIILRPEQEAAVEQTQKIFKKKDRMLWNAKMRFGKTLTSL
                     QLIKNEQFNKVLIMTHRPVVADSWFDDFKKMKMADEGYNYGSVNQGETLENLKKEDHP
                     FIYFASIQLLRYNNGQTNLKDFSDVDWNLIIIDEAHEGTQTELSDTVMKELLKEQTKV
                     LELSGTPFNLLDQFDDEQVYTWDYVMEQAAKKKWELEHPDKPNPYETLPKVSMYTFEM
                     KHKERFSDESKSFNFREFFRVNGAGELVHESSVRAFLDNITNPDSTTNYPFSTKSYRE
                     ELRHTLWLMPGVKEANAFEDLLKEHPIFGKEYAIVNVVRGSSSDDGVANEVDIEKVRH
                     AITDDPSATKTITLTVRKLTTGVNIPEWTAVLFLSNTNSAMNYLQAAFRAQTPFSHEK
                     LGMKKNCYVFDFAPDRALTVMAESAQINSGVGKKNTQEQKQAMARLLNFMPILGVSDN
                     GMKTFDVDRMLTQLKKVYAEKAVRSGFEDDSLYNDHLLTLTSDDESMFNELNAIVGKT
                     QSQKTPKKVVINENGLTDAENEQAERAKKKPKAKRTAEDLELIEKQKEAKKQRKNLIS
                     ILRGVSIRIPMMIYGMKVDLSKDITIKDFIKQVDNESWKEFMPKGFTKGMFQDITKYY
                     DAEVFIEAGRIIRQRAKSFDDLDFIERAEHIAELFGTFKNPDKETVLTPWRVVNMQIT
                     KSIGGLNFYDDNFQSVTDGATSNLHWVDNQLTSSVYYQDVKILDLNSKTGLYPLHSAI
                     SLYYQRMIENDDSRFEADRVYQEILANNVYAIAKTPMAKTITERTLVGYRDYKTNVVY
                     IENLTEILKSTQDKGKEIVEEAFNQVKFDIVIGNPPYQESTNENIMRTGQQKRVKNVF
                     QDFQLLADNLALKYSCLIYPGGRWVHQSGKGMKEFGKNQINSQHLRQLIYYPDAGEIF
                     GEVAIPDGISVVVKDYQVERETFDYTYSYAGEEVTVTRSHPGDKLMPLNPSNEAIVEH
                     IEVFMRNYHLKPLSDSKVINQKLFQVESDFAELNPDKVILDQGQSFDVSHQIKLLTND
                     KAGKAGRATWFIAERDVIKVNKDLIDKWKVVVSSANAGGQKRDNQLEILPPQTAFGRS
                     RLALKMFDSQKEAENFYQYVSSYVIRLAFLMTDESLTSLAKVVPDILDYSDSNNLLDF
                     EKNIDEQLQTLLELTDEQMVYIKEVVDSLRK"
     gene            20113..20601
                     /locus_tag="VBD11_000518"
     CDS             20113..20601
                     /locus_tag="VBD11_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953493.1"
                     /translation="MEPYITESIANVGVELGILAVKGTTAKINSKIQSMKTEREADKL
                     RASYDDIVNELLSERADALRIAQIYKEEYEKVTIKDEDIEYLHNTLKRIISLLGTFGN
                     VTKEQSVSFQQMTELLNKDTLKTMQLLGFNYREAIGEPLTEVCANTIKSRLGNFSKSK
                     KK"
     gene            20653..>21131
                     /locus_tag="VBD11_000519"
     CDS             20653..>21131
                     /locus_tag="VBD11_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002284916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage resistance protein"
                     /protein_id="HEO6953494.1"
                     /translation="MDMKAFQAKDEIIVSEAVQSKITANALFNFMREYSFLEKVIFNM
                     AISPRYYPEDISYLNLRYNDKDLTEWYIPMTCFCDIPLHQISYHAEGNSLSSNDKGYG
                     KFSIAFHKKFGIEKGIQPIHYLNENSAHVEELTNTMDLLLNQGGNSMEVNEVLTNFI"
BASE COUNT         7062 a         3312 c         4343 g         6414 t
ORIGIN      
        1 tagaatccta tgctaaagcc aatcctaatt ttaaacttca cttaaataat agtagcctcc
       61 aaggaagact tgattttagt cagtctgttt ttgaaggaca accaacgata tttatgtgtg
      121 ggccaactag tatgacatct acttatgcta aggtctttcg gcaaaaagat gcaaagtctc
      181 gtttagtcta tgaaggattt tcctttagag acagttggtt atccatattt ctattaaaaa
      241 cttttgacaa agtttattct aatttgataa aataagaggg tctataagac aaaccaactt
      301 tttcttggtt ttaggagtgc caatcctaag actcggatga agcaataaaa ggagaatata
      361 ttatggcaat ctcaaaagag aaaaaaaatg aaatcattgc tcaatatgca cgtcacgaag
      421 gtgatacagg atcagttgaa gttcaagtag cagttcttac ttgggaaatc aaccacctta
      481 atgaccacat caaacaacac aaaaaagacc acgctactta ccgtggattg atgaaaaaaa
      541 ttggtcaccg tcgtaactta ttagcatacc tacgtcgtac agatgttaac cgttaccgtg
      601 aattgattca atcacttgga cttcgtcgtt aatagacaac agagtcaagt aaaaacgcta
      661 gaaaattttc tagtgttttt tgttttaaac gtactatgaa cgtaagtact ttgagggggc
      721 caacagtaag aaagtgtttt aacctatgaa gagggttatt aagcaagcta agattaaccc
      781 tagaaattta ttttaatcat tgtaacaagg tctgattgct agtattaata atcagacttt
      841 ttacgtttac attaatgaat gataatacag cttcccccac ggattttcgc tactcaattt
      901 tatagaatta tgttagaata aagcttatac gtggttttat gcaaagttga gtttagaaca
      961 gctagattga actttgtagg aaacgcgtaa acaaaaaata tggagaataa tatgtcaaaa
     1021 caagtctttg aaatgatttt tgctggtaaa aaattagttg ttgaaactgg ccaagtggct
     1081 aaacaagcaa atggatcagt tgtcgttcgt tatggagatt ctactgtact tacagcagca
     1141 gttatgtcta agaaaatgtc aacaggtgat tttttcccac ttcaagtcaa ttacgaagaa
     1201 aaaatgtatg ctgctgggaa atttccgggt ggttttaaca aacgtgaagg gcgtccaagt
     1261 acggatgcta ccttaacagc acgtcttatt gatagaccaa tccgtccgat gtttgctgag
     1321 ggatttcgta acgaagtcca ggtcatcaat actgttcttt catttgatga aaatgctagc
     1381 gcaccaatgg ctgctatgtt tggttcatca ttagcattat cgatttccga tattcctttc
     1441 aatgggccaa ttgctggagt tcaagttgct tatgttgatg gcaattttat tattaatccg
     1501 acagcacaag aacaagaggc ttctgcgtta gaattaacgg ttgcaggtac taaagaagcg
     1561 attaacatgg ttgaatctgg agcgaaagaa ctttcagaag aaatcatgct agaggctctt
     1621 cttaaaggac atgaggctgt ctgtgaatta atcgcctttc aagaagagat tgtaactgct
     1681 attggtaaag aaaaagccga agtagaactt cttcaagttg atccagaact tcaagcagaa
     1741 attattgcta ctcataacat agctttacag gctgcagtac aagttgaaga gaaaaaggct
     1801 agagaagcag ctacagaagc agttaaagaa gtagttatag gtgaatatga ggcgcgttac
     1861 gctgaacatg aagaatatga tcgcattatg cgtgatgttg ctgaaatttt agaacaaatg
     1921 gaacatgcag aagttcgtcg cctcattaca gaagataagg ttcgtcctga tggacgtcgt
     1981 gtggatgaga ttcgtccatt agatgcagtg attgattttt taccacaagt tcatggatca
     2041 ggtctcttta cacgaggaca aacccaagca ttatcagtat taacgttagc acctatgggt
     2101 gaagcacaaa tcattgatgg gctaacacca gagtataaga aacgctttat gcaccactat
     2161 aacttcccac aatattcagt tggtgagacg ggacgttatg gagctgcagg gcgtcgtgaa
     2221 attggtcacg gtgctttagg agagcgtgca ttagaacaag tccttccaag cttggaggag
     2281 tttccgtatg ctatccgact agttgcagaa gttttagaat caaacggttc atcatctcag
     2341 gcttctatat gtgctggaac gcttgctctt atggcaggtg gtgtgcctat aaaagctccg
     2401 gttgctggta ttgccatggg actcatatca gatggaacaa actacacagt ccttactgat
     2461 attcaaggat tagaagacca ttttggcgat atggacttta aggttgcggg tacacgtgaa
     2521 gggattacag cacttcaaat ggatattaaa attgaaggta ttacccctca aattttggaa
     2581 gaagctcttg cgcaagctaa aaaagcacgc tttgaaattc ttgatgtcct tcatggagct
     2641 atcgcagagt cacgtccaca attagcacca acggcaccta agattgatat gataaaaatc
     2701 gatgttgata agattaaagt tgtcatcggt aaaggcggcg aaactatcga taaaatcatc
     2761 gctgaaacag gtgttaagat tgatattgat gaagaaggaa atgtatctat cttctctagt
     2821 gatcaggctg ctattgatcg tacgaaagac attattgcaa gtttagtacg tgaagcaaaa
     2881 gtgggagaag tttaccatgc taaagtagtt cgtatcgaga aattcggtgc ctttgtcaac
     2941 ctctttgata aaacagatgc attggtacat atttcagaaa ttgcatggac acgtacagca
     3001 aatgtagctg atgttcttga aattggtgaa gaagttgatg tgaaagtcat caaaattgat
     3061 gataaagggc gagttgatgc ctctatgaaa gcattattac cacgtcctcc aaaagctgac
     3121 aatcctaaaa aggaatctta atatgacaag tacaaacgag ttagacatta gattgcgtgc
     3181 ttttatcaat gcaccggata attttttaga tagtataggt cttgtgaatg ccttgcacca
     3241 ctctacggtt tgggcaagca aggaacccta tgctattcaa gtagatggtc aagaagttgt
     3301 tcctgttttt acggatatta ctgaccttaa tcatttcaaa gaggagcaag aaagtgctcg
     3361 agatatgttt tgggagagtc gtcgtagttt agacgttctt gatgaagcta tttcacatgg
     3421 cttagcagga ttggtctata atttaaaaaa agaaggcgat tttgggaact cgactatttt
     3481 ctactgtgaa gatatggtgc aatttatgaa caattacacc acaatcttga atcagttgtt
     3541 aaatgaagat aacattgttg ccgatatcat ggataaaacc tacttggtac ctgcctttgt
     3601 ccatccacgt gaagaaggta gttttgaccg ttttttccct acaatgtcta cccccgaagg
     3661 aaaaagttat gttccagtat tctcaaacct tttaagtttt gaaaaatggt ataaccataa
     3721 tgattttggt ggtgctttta ggaaagcaca aggagtaatt ttagcctgga cgattgacga
     3781 tatttataaa ccgcgtaatg gagaaaatga gattgatgat acctttggag tagccatcaa
     3841 tccatttgat gaacaacaag ttttagttga ttggtctgat gtagaaaacg attgaggaaa
     3901 actatggggt ggtggaaaga aagtatagct attgtcaaag aacaagatcc agctgctcgt
     3961 agttctctag aagttatttt aacttatcca ggaataaaag ctcttgcagc tcataggcta
     4021 tctcattttt tatggaatca taattttaaa ttgttagcac gtatgcacag tcaattttgg
     4081 cgtttctgga cacaaattga aattcatccc ggagcgacga tttcggaagg ggtttttatt
     4141 gaccacggtt caggattggt cattggagaa actgccattg tagagaaagg tgctatgcta
     4201 taccatgggg ttacattggg tggcacaggt aaagacaaag gcaaacgaca cccaaccata
     4261 cgtaaaggtg cgctaatttc agcacattct caaatcatcg gtccaattga ggttggagaa
     4321 aatgctaaag tcggtgctgc agcagttgtc cttgcagatg tgccagcaga cgtaacagta
     4381 gttggtgttc cagctaaagt tgtccgtgtc catggtcaaa aggatgatct tcaaatccga
     4441 agtattgaac atgatcgaga agaaagttat tattcgtcca aactttagga ggtatttgtg
     4501 aaggattatg taacaactca aatattatca attttaggta tactagcagg agcttgttta
     4561 agctatttaa agtttgcaca aggtaactct ctcttaggaa ttatttgggc tgcctttgct
     4621 ttaatgtata ttgttcgttt gtataaagct tatcaaaaac agaaaaagga aaaaccatga
     4681 taaaaattta cgacactatg actcgcagtt tacaagattt tatacctctc aatgaaggta
     4741 aagtcaacat gtacgtctgt gggccaacag tttataacta tatacacatc gggaatgccc
     4801 gcagtgtagt agcttttgat actattcgtc gttattttga gtattgtggt taccaagtca
     4861 attatatttc taactttact gatgttgatg ataaaattat aaaaggtgca gctgaagcag
     4921 gtatggatac aaaatccttt tcagataagt ttatttcagc atttatggaa gatgttgctg
     4981 cattaggtgt caaacctgct acaaaaaatc ctcgagttat tgactatatg gatgagatta
     5041 ttgattttgt taaagtttta gttgataagg aatttgccta tgaagctaat ggagatgttt
     5101 attttagagt aagtaaatca catcattatg caaaattagc caataaaact ttggaagatt
     5161 tagagattgg tgcaagtggt cgtgttgatg gggaaggaga gattaaagag aatcctttag
     5221 attttgcact ttggaaatca gctaaatctg gggaagtctc atgggaaagt ccttggggga
     5281 aagggcgccc agggtggcat attgaatgtt ctgttatggc aactgagatc cttggtgata
     5341 ctattgatat ccatggtgga ggagctgatt tagaatttcc acatcacacg aatgagattg
     5401 cacaatctga agctaaaaca ggtaagacct ttgcaaatta ctggatgcat aatggttttg
     5461 ttaacgttga taacgagaaa atgtctaaat ctcttggtaa ctttattact gttcacgata
     5521 tgttgaagag cgttgacgga caagtaatac gttttttctt agcgacacaa cagtatcgta
     5581 aacccgtgaa tttcactgaa aaggccgttc atgatgcgga agttaacctt aaatatttaa
     5641 aaaatacttt caacttacca atccaagaga atgctaatga tgaagaatta gaacaatttg
     5701 tgaaagcatt tcaagaagcg atggatgatg attttaatac agcaaacggt attactgtta
     5761 tctttgaaat ggcaaaatgg attaattcag gacactatac ttcaaaagtt aaggaaaccc
     5821 ttgctgaatt attagaaatt tttggtattg tattccaaga ggaagttctt gatgctgata
     5881 ttgaatcttt aatagaacaa cgtcaagaag cacgagctaa tcgtgatttt gcaactgctg
     5941 atcgtattag agatgaatta gcaaaacaag gaattaaatt gttagatact aaggatggcg
     6001 tgaggtggac tcgtgattga tgttcgtttg attaatggta ttgctttagc ttttgaagga
     6061 gatgcagttt attctttgta tatccgacgc catttaatta tgcagggttt taccaaacct
     6121 aatcagttac atcggaaagc aacccaatat gtttctgcta atgcccaagc attgttaatt
     6181 aatgccatgt tagaagaaaa tattttaact gatgaagagc agttaattta taaacgagga
     6241 cgtaatgcga atagtcatac aaaagctaaa aatgctgata ttattaccta tcgtatgtct
     6301 acaggttttg aggctcttat ggggtacttg gatatgactg gtcagataaa gcgcttggaa
     6361 actctaatac agtggtgtat tgaaaccatt gaaaaataaa caaaaagaga ggctatatcc
     6421 tctctttttt atagagacat taggatttgt tggtaaaata ttgctataaa aagctttatg
     6481 ctatagcatt catgatataa tatgactatg aaagataaac aatttaaaga agaatcaagt
     6541 gaccttgtct atggcttaca tgctgtgaca gagagtttaa gagctaatac tggtaataaa
     6601 ttatacttgc aagatgactt aagaggaaag aacgttgata aagtcaaagc actagcgact
     6661 gaaaaaaaag tatccatttc ctggactcct aaaaaaatct tatcagacat gacaaatggt
     6721 ggagttcacc aaggattcgt tctaaaagtt tcggaatttg cctatgcgga tttatcagag
     6781 ataatgacaa aggcagagaa tgaagaaaat cctctaattt taatccttga tggtctgacc
     6841 gaccctcata acctaggttc tattctacgc acagctgatg caaccaatgt aacaggcatt
     6901 atcatcccca aacatcgttc tgttggagtg accccggtgg tttcaaagac atctacagga
     6961 gctgttgaac atgttcctat cgcaagggta actaatctca gtcaaacttt ggatacttta
     7021 aaagataagg acttctggat atttggcact gatatgaatg ggacaccatc gcataagtgg
     7081 aataccaaag gtaaattagc cctagttatt ggtaatgaag gaaaaggtat ttctcataat
     7141 atcaaaaagc aagtagatga aatgattaca attcccatga atggtcatgt tcagagttta
     7201 aatgccagtg tcgcagctgc tattttgatg tatgaagtat ttagaaaccg cctatgaaaa
     7261 aacattctat tttactcgtt gatggctaca atatgattgc tttttggaaa gatactcgac
     7321 agctttttaa aagcaatcgt ttagaagagg caagggaagt attacttaga aagctaaatc
     7381 attacgcaca tttcgaacac attgatatta tttgtgtatt tgatgcccag tatgttcctg
     7441 gtgtacgtca acgttatgac caatacaaaa tatccgttat ctttactgag gaggacgaaa
     7501 ctgcggatag ctatattgaa cgagcagcgg cagaacttaa tcaatctgtg ttaaatttag
     7561 tctccgttgc taccagtgat ttaaatgaac aatggactat attttctcag ggtgctttac
     7621 gtgtctcagc tagagaattg gaacagcgtg tagcgacagt aaaatcagat ttggataaga
     7681 tgtccagtca aatcgatttg agtacgccaa agttaaggcc gtggaatgat gaacaactag
     7741 gaaaactcaa ggatttttta gatggtatgt aaaaactaac actttattag ccagccaaca
     7801 atttttttga tataatattg gaactacttt gattatcaaa taaaaataat agattgaggt
     7861 aacaatgact tttaaaatac taacggattc gacatcggat ttagatgaaa agtgggctca
     7921 agagcacaat gtcgatatta taggtttaac cattgaactt gatggaaaga cttatgagac
     7981 agtcggagac gaaaaaatta ctagtgactt cttgttggag cgtatgcaag agggagcaaa
     8041 gcctacaaca agccaaatta acgttggtca gtttgaagaa gttttttcaa cctatgcaga
     8101 aaatgaccat gccttgttat atttagcgct atcttcacat ttatcaggga cttaccagag
     8161 tgcaactatt gcgcgtgaga tggtcttaga caaatatcct gatgctcaaa ttgagattgt
     8221 ggatacaatg gcagcttcat gtggtgaggg tgtcttagcg atgttagcaa ctaaagagcg
     8281 acaagaaggt aagtctttag aagaagtgaa acaaaaaatt gaatcacttc tccctaaatt
     8341 aaacacatac tttttagttg acgatctcaa ccatttaatg cgtagtggtc gcttatcaaa
     8401 aggtgcagct atcattggaa gtgttgctaa gattaaacca ttacttaagt tagatagtga
     8461 gggtaaatta gtaccatttg ccaagacaag gggccgtaaa aaaggtatta aagagattgt
     8521 gactcaggca acaaaaacac tatcttatag cactttaatt attgcttata gtggagagaa
     8581 agattcagct caagttatga aagaacagtt attagcagat gagcgtattg aagaagttat
     8641 tattcgtcca ttagggccag ttatttcagc tcatgtgggc tcaggtgctt tagctctatt
     8701 ctctttaggg gaagaaaacc gataacaaat caaagggcat aacagtacgt tatgttcttt
     8761 tagtatacat tttataaaat gtattttata tgatgctatg aaactataaa atagtaggag
     8821 acatattatg aaaaataaaa taagaactat cctcaatatg atgaacgaca acaacaattg
     8881 gttttacaaa gttatcaaaa atgtttttag acgatgtttt ttatcttatg gcttgaaagt
     8941 gtttttggaa agctaataaa gggaagcgtg tcacctgatt ttataataat attaacttta
     9001 tttagtggta tttctatttt gttacgactt ctaatctaaa aagagtctct ccgtttgttg
     9061 atgaaagatt ttggaaaatg tttttgttgc tggcgcattt gccctcatag gaataactat
     9121 tttattgatg cttttactag gaagttgata ttgctactta tttttcaagg ggaaatgatg
     9181 caattttagc agttatagga atatcattga tagctatggg aacttcagta tactacctta
     9241 agtataagga cagatagtaa tatgaaaaat ttacgcttaa agacagctag agctagtatg
     9301 gatttattac aacagtctct agctgaaaag gtcggtgtat catgtcagac aatagccgcc
     9361 attgaaaaag gaggctataa ttaagcgatt aaattgtgtc aagctttgaa taaaactgta
     9421 gatgatttat tttgggatga aaattgataa aataaaagaa ataacttgac gaatgctacc
     9481 ttattttgat atgattataa gcggtattgt ttaccccatt tgtaaggccc cggaaccttt
     9541 caaatactct cgtggaccgg aacatccaca cctgtaaaca aaaacgaatt cgtataggag
     9601 aaatcatgaa caaaacaact ttcatggcta aaccaggcca agttgaacgc aaatggtacg
     9661 ttgttgacgc aacagatgta cctcttggac gtctttctgc agtagttgct agcgtacttc
     9721 gcggaaaaaa caaaccaact ttcacaccac acactgatac aggtgacttt gtgattgtta
     9781 tcaatgctga aaaagttaaa ttaactggta aaaaagcatc agataaaatc tactacactc
     9841 actcaatgta tccaggtggt ttgaaacaaa tctcagcagg tgaacttcgt tctaaaaatg
     9901 ctgttcgttt gattgaaaaa tcagttaaag gcatgcttcc acataacact cttggacgtg
     9961 cacaaggtat gaaattgaaa gtatttgttg gcggtgagca tacacatgct gcacaacaac
    10021 cagaagtact tgatatctca ggacttatct aagaggaaag gagcattata taatggcaca
    10081 agcacaatat gcaggtactg gtcgccgtaa aaacgctgtt gcacgcgttc gtttggttcc
    10141 aggtactggt aaaattacaa tcaataaaaa agatgtagaa gagtacatcc cacacgcaga
    10201 ccttcgtctt gttatcaacc aacctttcgc agttacttca actcaaggtt catacgatgt
    10261 ttttgttaac gttgtaggtg gtggatatgc aggtcaatca ggtgcgatcc gtcatggtat
    10321 ctcacgtgca cttttagaag ttgacccaga tttccgcgat tcattgaaac gtgctggact
    10381 tcttacacgt gacgcacgta tggttgaacg taaaaaacca ggtctcaaaa aagcccgcaa
    10441 ggcttcacag ttttcaaaac gttaatatcc agttatatca acgtttcaga gcactttgcg
    10501 aagatttcgc aaggtgcttt tttgttgaaa aaagcgcctt ggtaatcctt ttggtaatct
    10561 gttgagacct agttttaaga gcggttaagt cagaaaagtt atttagcttt attgaggtaa
    10621 tccgcaaact ttttaatgga ttgtttttct cctgattttg tagcatgact gtaagtatct
    10681 aaagtcatct ggctgctagc atgtcctaga cgtttagcag tatttacagg gtcaattccc
    10741 atttcaatca tcaaagtcgc atgagtgtgt ctaaaaccat gtggactaat ctttttaaga
    10801 tgatgtttcc gtataatgcg ttttagaata ttgttgatgt agtcagcatg caagggttct
    10861 atctcaccat ctcggttgca gtatgtaaac ataaactctt tatcgataga aagaggtgtt
    10921 actgataatt gccccttttc tttgcatgtt tcttgtctcc atttgtttag gatagaacat
    10981 gtttcgtcat caagataaat ggagcgaact gagaaagtat tttttgtttc tttttcgatg
    11041 gcatggccat ctattctgcc tagacttttg ctgactgtta atagttggct atcagaatca
    11101 aaatcttccc acttgagagc atatagttct ccttttcgta acccactata agctagtaat
    11161 ctaaatagag cataatgttt atagggttca ttttccaaaa tgattgtcaa aaattgatgt
    11221 aactcgttaa ctgtccagta attctcactg acagtttttt tgaatttcgg cataataacc
    11281 ttactcatag gattgcgctc aatatagttc atgttaatag caaattcaaa tattttagaa
    11341 gtataagatt tgatttgctt catgttttta aaagtcttag ctttgttagt gatgaaggtt
    11401 tgacagtcta aaggactgat ttttgaaatt ttcttttctc caaaaacagg aagaatatgt
    11461 aggcgataaa ggtcggcagt ttttgatgca gtagttgctt caacggtatc tttataagct
    11521 tcataccacc tttgatataa ctcttggtaa gtataggttg tttgagcagt aagatgaaca
    11581 aatgtcccac tattcaactg atgattaaca gcagtttcat gtgctttagc agctttgcga
    11641 gttttgaacc cccgcttagt gtaatatttt ttcttaccat agtcatttag accaagataa
    11701 actttaacaa aataggtgat tccttttgtt gattggtact tatgtacaga cataacagtt
    11761 ctccgtttct aagttttcgg agcttatgta attgagaaac tgttagttat tttttgtgtc
    11821 gatcttcttg tctaattcgg tattcttcgg tctgaatcca ggtttgccaa atttctgact
    11881 ctcgttttaa actattcatg tgtttagtga aggttttcat gaactcttct tcatgattga
    11941 ccaagtaacg agctaaaatg cgtctaaaat cgtctgagaa accctcattt aagggcatag
    12001 acaaatgaat ctcccctaat tcaattcctt tcttcagctt ctccatatcg cttttaaaag
    12061 gtgttacaga actaatctcg gaaactttaa agctgtctgt gataatgtcc atagttgacg
    12121 gggcttgtct attatcgtca agtcctaaga tgtaagcaac agatactcca aaataatcag
    12181 ctaatttttg agcatttcct tttttgggtt gacgaatgcc ttgattgtat cccgataagg
    12241 taggataagc aatatttgta gcagaactaa tctcagtcag ttttttgcca ctctcttcta
    12301 tcagttttgc aatatgattg gtcataaata cctccttgag agtattataa cacatttaat
    12361 tctttttgaa taaaaatagt tgacataaat tctaaaagga tatataattc aaaatgaata
    12421 ataaataaat ttcggagttt atttaattga gaaatagaaa tgaggtaatc ttatgacgga
    12481 tgaaacgact ttagttgtac aactgccaaa atcattggaa agacagttgc gagcttatta
    12541 tgacgggatg gtcagcagtg ccattgaacg tgctttagag gataaggaac tctataagcc
    12601 gatggttagg atgtctgcct tatcccgctg gttagatgtt tcaacgacca ccattcaaaa
    12661 atggacacga gaaggaatgc ctacaatggt tattgatggt gtgactctgt acgataaacg
    12721 agcggtcacc cgttggttga agcaatatga aagatgaggt aaaggatggc taatagacga
    12781 atgttaagta agacagtggt gcagacacaa cgatttttgc gcctaccact tgaagcgcag
    12841 gcactttatt gtcacttggt agtcaatgcg gatgacgatg gtattgttga agcttttcca
    12901 attatgcgaa tgatcaatgc taatgaagat agcctaaatt tattaaccct taagcagtat
    12961 ttactcttgt taaataatga gatggtttac tttatcaagg atttctttga gcagaatacc
    13021 atcaaagcag accgttacac accgagtagt catcgtcaac ttcttataga ttatctgaag
    13081 gataatcaat tgttggagag cttaaatcta ggtccgcgct tggtcaatta tcttcaaaat
    13141 actcggctcc aagttggaac cagtctggat ccagatggga accgcaatat aattaaagat
    13201 aagataagta aagataggat aggtaaagat aagttaagtc aagataagat aggattagat
    13261 aatgatccaa tttcatcacg actgactgat gacatctggt taatggaaac gctatggcat
    13321 cgagaattaa cccaacagga gaaaacatta ctagaaggat taacggttaa tcagtcctta
    13381 ttgcagttag ctattcagaa aacagctgag cttgaaccaa ggaaacaaaa catgagttac
    13441 gtcaaaggga tactgacgaa ttgggaagct agagggctta taactgctga ccaagtgata
    13501 gcatctgaaa aagactacca cccattagaa atttcaaatg tggaagtctc agaagatttc
    13561 ttagcagcta tggatttatg gaaagattag gaggataact agagatgaca gtatattacc
    13621 aattagaaaa tatgttagtg gctgggttta agtcacaaga ggagtacaac tgctaccaag
    13681 aactcaagag agactatgaa agtgagacgg aggactacag cttttcgatt agggaaatta
    13741 caaatcagct tgaaatcatt tgtcaaacct cagagaatga ttttccagat ttagaagaag
    13801 gaatattaac tgactattta gaactagtag aacagttgaa acgatatgat actaagctgg
    13861 cagacaggta tttaaggatg atccatcatg tctgaacgtg taagaaatgt tttaaagaag
    13921 ttttatgtga cggagtcgca gaataaagtg cttaatcagt tagtagatga tacaggatta
    13981 cagacattct ctaattatgc tcgtcggatg ctccttaagg aaacgtcact ctttatgcga
    14041 tttgatgaat ctcagtttga tgaattgatt tattctctaa gacgtatcga aaataattta
    14101 aggcaattat ctaaaattgc agagcaatct caagatagtc aagcatacag agctatggag
    14161 tatagcagaa ggctagtttc aaattatgag aagcagttaa cacgttacca ccagaaaaag
    14221 aaacggaaac tactgtctaa gggggcataa tggctaaaaa gagaattaga aatgttataa
    14281 aacaggtctt tttatctgaa gaagaaaatc aaaaactgtt agagcgcatg aagcaagatg
    14341 gcatctcaaa tttttctctt tttgctagaa agcagttatt aaaacctgat tttgaagctt
    14401 gggtagtgag ttttccagaa tatcagtcgc tgacggatag gttgctatct attggtagaa
    14461 caattaattc tattgccaag agtgcaaccc agtttgggaa aatttcccga caagatttga
    14521 tggaacttgg tcagctaatg gaggagcatg tggaactagt ggagaagcag ttgaaggaag
    14581 ataagcaacg aatagctaag aagtgatagt cttccagtca ttttgatggc atagttttaa
    14641 aatatgatag caaaaacgct gatgatggtc agcgtttttt atcatgaagt cgttaaaaat
    14701 ctttgtgcgg acaatgtacg gcactgtata aagtacggca atgtctttaa aatgccaaag
    14761 gtctttgcga gctcagacag tgtatacaca ctatcgtaaa aatagaacca tcagtaaagg
    14821 tcagactgac actttttgac tgatgattct attttatttt ggggagaatc cccaaacccc
    14881 tgtaaatata aagatattta ctgaaacatg atataatagg aattaacaat aaaggtttca
    14941 aggagtaggt tttgaaagag aatttaataa aatcaaagca tagagttcaa aagcatggtg
    15001 aagtatttac accatcatgg atggttcaga aaatgctaga tactcctgga gtaaaagagg
    15061 catgtgaaaa tatctctgcg acattcttag agccatctgc aggagatggt aattttttac
    15121 aagctatttt agaaagaaaa ctcgcttctg taattaaaca atatgataaa cgaaattgga
    15181 agacgaaatc gttgatagct ctgtcatcta tttatggaat tgaatttctt gaagataatt
    15241 tagaagtggc gcgatcacgt atgttccttt attatttaga ttggtacgag ataacgtttg
    15301 gagatagatt gacgaataag agtgatattt ataaatctgc gtattatctg attcataaaa
    15361 atattgtaag aggaaatacc ttaactcaga gacatcctgt tacaggggat cttatttggt
    15421 ttaatgaatg gcaagtagta aaaggccacc ctagtctggt tagaaaaatt cctttttctt
    15481 tatcagaact tttaggaaaa gaagttgaaa atgataaaag tattgttgaa ggacagctta
    15541 gtttcttcga tatagatgat aattttgttg tagaagaaac tgctgaaaag atattggaga
    15601 gtaaagaaat aaatattcag aaagtttatt tgttaggaga ttaaggatga tatcaaattt
    15661 tgaattttta gctattgata tagatacgtc agaacttttt aaaaccatta acatggcgga
    15721 agaaaattat actcacggcg attacgaagg aacattaaca aaggttagaa aagttgctga
    15781 aaatacagct agattaattg ctgatagaga atatattgac attcgagagc gagcaacttt
    15841 taacgatgtt ttgaaggaca taagaggact tatccctaat aaaaaaattg tagataactt
    15901 ctatgaaata aagggtaagg ggaataattc agctcatata ttaaatccag aagacgccac
    15961 aaaagagaat gctttaaaat cattggaaca agtatttgat attttggctt gggttatgtt
    16021 gacttatatt gatcctgata tcaaagctag tgctattggt cgattcttgg agccaagggc
    16081 tcaagaaatg tacaagactg ctgagaggaa atttatttat attcagaccg ttgataatca
    16141 aagtggctta ttcaatgctt ttgaaggaac acaaaaaatt ggtgaaggta ctgtttcttc
    16201 agatgatata gagacggact ggtctcctaa tagtgagttt ttaagaacag ctgcaccaaa
    16261 acgtatcaat caatatatga aaacttcagg tttgccttat acacttggtt gggttgaact
    16321 tgcctataag aagtcaacaa aatcttggtt tcatgattac gatgtgcata atgttttaaa
    16381 acgttcaggt gttaagcaag cagagcaatt ggagggtaca gagtggttca agacagattt
    16441 agagacagct aagtctgcaa ttaaagcggt taaagaaggt aaaaattatc ttaatatccc
    16501 tgctgatgaa acagttgtgg aagagcctat catcatactt cgtcctgaac aggaggctgc
    16561 tgttgaacaa actcaaaaaa tctttaagaa gaaagatagg atgctctgga atgctaaaat
    16621 gcgtttcggt aaaactttaa cctctttgca gttgataaaa aatgaacaat tcaataaagt
    16681 acttatcatg acccaccgtc cagtagtagc tgattcatgg tttgatgact tcaaaaaaat
    16741 gaaaatggct gatgaaggct ataattatgg atcggtcaat caaggagaga cacttgaaaa
    16801 cttgaagaag gaagaccatc cctttatcta ttttgcttct atacaattgc ttcgttataa
    16861 taatggacag acaaatctaa aagatttctc agatgttgat tggaacctaa ttattattga
    16921 tgaagcacat gaaggaactc agacggaatt gagtgatacc gtcatgaaag agttgcttaa
    16981 ggagcagacg aaagttctag aattatcagg taccccgttt aatttacttg accaatttga
    17041 cgatgaacaa gtttatactt gggactacgt tatggagcaa gcagctaaaa agaaatggga
    17101 attggaacat ccggataagc ctaatccata tgaaacctta cccaaagtat caatgtatac
    17161 ttttgaaatg aaacataagg aacgtttttc ggatgaatcg aaatccttta actttaggga
    17221 attctttcgc gtcaatggag ctggagaact tgttcatgag tctagtgttc gagcattttt
    17281 ggataatatt acgaatccag atagcacgac aaattatcca ttttcaacaa aatcttatcg
    17341 agaagaattg cgtcataccc tttggttgat gcctggtgtg aaagaagcaa atgcttttga
    17401 agatctacta aaggaacatc ccatttttgg taaagaatat gctattgtta atgtcgttcg
    17461 tggttcgagt agtgatgatg gtgttgcgaa tgaagttgat attgaaaaag ttcgtcatgc
    17521 tataacagac gacccatcag caacaaaaac catcactttg acggttcgca agttaacaac
    17581 aggggtcaat attccagagt ggacagctgt tttattcttg tcaaatacca atagtgccat
    17641 gaattacctt caggctgcgt ttcgtgctca gacacctttt tctcatgaaa agctaggaat
    17701 gaagaaaaat tgttatgttt ttgattttgc accagataga gctctaacag ttatggcaga
    17761 gagtgctcaa ataaattcgg gtgtaggtaa aaagaatact caagagcaaa aacaggcaat
    17821 ggctcgtctg cttaatttca tgcctatttt aggagtctct gataatggta tgaagacttt
    17881 tgatgtggac agaatgttga ctcagcttaa aaaggtctat gctgaaaaag cagtacgttc
    17941 aggctttgaa gatgatagtc tctacaatga ccacctgctt actcttacat cagatgatga
    18001 atcaatgttt aatgagctaa atgctattgt tggaaagaca caaagtcaga agacacctaa
    18061 aaaagttgtc attaatgaaa atggtctgac tgatgctgag aatgaacaag ctgagcgtgc
    18121 taagaagaaa ccaaaagcaa agaggacagc ggaggatcta gaacttatag aaaagcaaaa
    18181 agaagctaag aagcaacgta aaaatttaat ttccatatta cgtggagttt ctatccgtat
    18241 tcctatgatg atttatggaa tgaaagtaga cctgtctaag gatatcacga tcaaagactt
    18301 tatcaagcaa gttgataatg aatcctggaa agagtttatg cctaagggct ttaccaaggg
    18361 aatgtttcaa gatattacca agtactatga tgccgaagtc tttattgaag caggccgtat
    18421 tatccgtcaa cgagcgaaat cttttgatga tttagacttt atagagcgcg ctgagcatat
    18481 tgccgagcta tttgggacat ttaagaatcc tgacaaagaa actgttttga caccttggag
    18541 agttgtgaat atgcagatta ccaagtctat aggtggtctg aacttttatg atgataattt
    18601 tcagtctgtc acagatggtg ctacttccaa tctccactgg gtagataatc agctaacatc
    18661 atcagtttat tatcaagatg ttaaaatttt agacctcaac tcaaaaacgg gactctatcc
    18721 tttacatagt gccattagtt tgtattatca acggatgata gaaaatgatg atagtcgatt
    18781 tgaagcggat cgagtttatc aagaaatatt agctaataat gtatatgcta ttgctaaaac
    18841 accaatggct aagacaatca ctgagcgaac attagtaggc tatcgagatt ataagaccaa
    18901 tgtggtttat attgaaaact taacggagat actcaagagt acacaggata agggaaaaga
    18961 aattgtagag gaggcattta atcaagtgaa atttgatata gttattggca atccgccata
    19021 ccaagagtct acaaacgaaa atattatgag gactggtcaa caaaaacgag taaaaaatgt
    19081 tttccaagac tttcaattat tagcagataa cttagctctg aaatatagct gtctcatata
    19141 tcctggtggg cgttgggttc atcagtctgg aaaaggaatg aaagaatttg gtaaaaatca
    19201 aattaatagt caacatttaa gacaattaat ttactatcct gatgctggtg aaatatttgg
    19261 cgaagttgct attccagatg gtatttcagt tgttgtcaaa gattatcagg tagaaagaga
    19321 gactttcgac tacacctata gttatgccgg tgaggaagta actgtgacta gaagtcatcc
    19381 aggtgataag ctaatgcctt taaatccaag taacgaggca attgttgagc atattgaagt
    19441 ctttatgaga aattatcatc tgaaaccatt atcagacagt aaggtgatta atcagaagct
    19501 ttttcaggtt gaaagtgact ttgcagaact aaatcctgat aaagttatcc ttgatcaagg
    19561 gcagtctttc gatgtttcac atcagattaa actcttgact aatgacaaag caggaaaggc
    19621 gggacgtgca acttggttta ttgctgaaag agacgtgata aaggttaata aagatttaat
    19681 tgacaagtgg aaagttgtgg tttcaagtgc caatgctggt ggtcaaaagc gtgataatca
    19741 acttgagatt cttccgccac agactgcttt tggccgaagt cgtctagctt taaagatgtt
    19801 tgatagccaa aaagaagcag aaaactttta tcagtatgtc agctcctatg tgatacgtct
    19861 tgcctttttg atgaccgatg aatccctcac ctctttagct aaagtcgttc cggatatttt
    19921 agactattca gatagtaata atttacttga tttcgaaaag aatattgatg aacagttgca
    19981 aactcttctg gaactaacag atgaacaaat ggtctatatt aaagaagtag tagatagttt
    20041 aaggaaataa tggtctatct gttggtaatg tggaagttat tgaaagtaaa gtgaaggaga
    20101 taaagtagga ttatggaacc ttatattaca gaatcaattg ctaatgtcgg tgttgaatta
    20161 ggtattttag cagttaaagg aacaacagct aaaatcaata gtaaaattca atcaatgaag
    20221 actgagcgtg aggctgacaa gttgagagct tcttatgatg atattgtaaa tgagttacta
    20281 tctgaacgag ctgatgcttt aaggattgcg caaatctata aagaagagta cgaaaaagtt
    20341 accattaaag atgaggatat tgaatatctt cataataccc ttaaacgtat tatttcttta
    20401 ttggggactt ttggtaacgt gacgaaagag caatctgttt cgtttcaaca aatgacagag
    20461 ctgttaaata aagacaccct aaaaacaatg caattattag ggtttaacta cagagaagca
    20521 atcggtgaac ctttaaccga agtttgtgcg aacactatca aatctagact agggaatttt
    20581 tctaaaagca agaagaagta aaactatgat aggggaactg ggtgaattaa tttattttag
    20641 aggtttttga caatggatat gaaagctttt caagcgaaag atgagataat agtctctgaa
    20701 gctgtacaat caaagattac ggcaaatgcg ttatttaatt ttatgagaga gtactccttc
    20761 ttagaaaaag ttatatttaa tatggcaatt tctcccagat actaccctga agatattagt
    20821 tatttgaact taagatataa cgacaaagat ttaactgaat ggtatattcc tatgacatgt
    20881 ttttgtgata ttccactaca ccagataagc tatcatgcag agggaaattc attatcatca
    20941 aatgataaag gatatggtaa attttcaata gcttttcaca aaaaatttgg aatagaaaaa
    21001 gggatacaac caattcatta cctaaatgag aattctgctc atgtagaaga actgacaaac
    21061 actatggatt tattattaaa tcaaggaggc aactccatgg aagttaatga agtcttaact
    21121 aactttattt t
//
LOCUS       DATROY010000014        20870 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.014, whole genome shotgun sequence.
ACCESSION   DATROY010000014 DATROY010000000
VERSION     DATROY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20870
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..884
                     /locus_tag="VBD11_000520"
     CDS             <1..884
                     /locus_tag="VBD11_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953495.1"
                     /translation="KTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKE
                     IINSAKKRTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVK
                     VFYNNQNLNWDAAINHEQERKQAILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTP
                     SRLWTNLFVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLL
                     ALGHHFDYQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLM
                     LIVTEYYIPYKIKKMID"
     gene            942..1742
                     /locus_tag="VBD11_000521"
     CDS             942..1742
                     /locus_tag="VBD11_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6953496.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            1732..2367
                     /gene="trmB"
                     /locus_tag="VBD11_000522"
     CDS             1732..2367
                     /gene="trmB"
                     /locus_tag="VBD11_000522"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6953497.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            2411..2498
                     /locus_tag="VBD11_000523"
     tRNA            2411..2498
                     /locus_tag="VBD11_000523"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2445..2447,aa:Ser,seq:gga)
     gene            2856..3332
                     /gene="rimP"
                     /locus_tag="VBD11_000524"
     CDS             2856..3332
                     /gene="rimP"
                     /locus_tag="VBD11_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6953498.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            3359..4519
                     /gene="nusA"
                     /locus_tag="VBD11_000525"
     CDS             3359..4519
                     /gene="nusA"
                     /locus_tag="VBD11_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6953499.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            4541..4837
                     /locus_tag="VBD11_000526"
     CDS             4541..4837
                     /locus_tag="VBD11_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6953500.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            4830..5132
                     /locus_tag="VBD11_000527"
     CDS             4830..5132
                     /locus_tag="VBD11_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6953501.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            5152..7935
                     /gene="infB"
                     /locus_tag="VBD11_000528"
     CDS             5152..7935
                     /gene="infB"
                     /locus_tag="VBD11_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6953502.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            8025..8393
                     /gene="rbfA"
                     /locus_tag="VBD11_000529"
     CDS             8025..8393
                     /gene="rbfA"
                     /locus_tag="VBD11_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6953503.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(8465..9469)
                     /locus_tag="VBD11_000530"
     CDS             complement(8465..9469)
                     /locus_tag="VBD11_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6953504.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            9632..10048
                     /locus_tag="VBD11_000531"
     CDS             9632..10048
                     /locus_tag="VBD11_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6953505.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            10061..12295
                     /locus_tag="VBD11_000532"
     CDS             10061..12295
                     /locus_tag="VBD11_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6953506.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            12336..12542
                     /locus_tag="VBD11_000533"
     CDS             12336..12542
                     /locus_tag="VBD11_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6953507.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            12652..13266
                     /locus_tag="VBD11_000534"
     CDS             12652..13266
                     /locus_tag="VBD11_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6953508.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            13281..14093
                     /locus_tag="VBD11_000535"
     CDS             13281..14093
                     /locus_tag="VBD11_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6953509.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKETTQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            14206..16848
                     /gene="polA"
                     /locus_tag="VBD11_000536"
     CDS             14206..16848
                     /gene="polA"
                     /locus_tag="VBD11_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6953510.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANVMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            16878..17318
                     /locus_tag="VBD11_000537"
     CDS             16878..17318
                     /locus_tag="VBD11_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6953511.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            17400..17879
                     /gene="perR"
                     /locus_tag="VBD11_000538"
     CDS             17400..17879
                     /gene="perR"
                     /locus_tag="VBD11_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6953512.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            18032..19597
                     /locus_tag="VBD11_000539"
     CDS             18032..19597
                     /locus_tag="VBD11_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO6953513.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAGKKGQALLDQIKANGSHTYQATITI
                     YGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            19710..20396
                     /locus_tag="VBD11_000540"
     CDS             19710..20396
                     /locus_tag="VBD11_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953514.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            20398..>20870
                     /locus_tag="VBD11_000541"
     CDS             20398..>20870
                     /locus_tag="VBD11_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953515.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNY"
BASE COUNT         7091 a         3208 c         4267 g         6304 t
ORIGIN      
        1 caaaaacaca ttggccaatt atcgtgattg tgtcaatcat tatcttaatg ttactagcga
       61 tgggggaaat agccagctat ttagaaccgg ctgataaaca atttttactt ataaaagaag
      121 aagcaataaa ggaaattata aattctgcta aaaaacggac ttatatcttt tggttggttg
      181 ttcaaacctt atttttagta ttaatatcac caattttgat aaagttaggc ttgtcagttt
      241 ttatgattac attgttaatt tttggtctag gaataatcaa atggttggtg ataacttaca
      301 aggtaaaggt cttttataac aatcaaaatt tgaactggga tgcagctata aatcatgagc
      361 aggaaagaaa acaggctata ttaaaatttt tctctttgtt tacaaatgtt aagggaattt
      421 cgacaagtgt aaaacgtcgt tcatttttag atgttatttt aaaacttatt tctaaaacgc
      481 catcgcgttt atggactaac ttatttgtga gggcatttct aagaagctca gattatcttg
      541 gtttaaccat aaggttggta actttaaata tcttatcagt catctttgtt aacgaaacct
      601 acctcgccct tgctctggca tttgtattta actacttatt attgttccaa ttattagctt
      661 taggtcacca ttttgattat caatacatga atcagctcta tccagttaga ttaaatgcta
      721 aagcaagtca gctaaaaggt tttctcagag tgttatcata tgctgtgaca gtaattgata
      781 tcatacttat aagagaattg aaacctgtta tattattgat agttttgatg ttaattgtga
      841 cggaatatta catcccttac aaaataaaga agatgattga ctaaagccta taaaacaagt
      901 aaaataagag gaaacgacta aatgaaaagg agggactgct ggtgacaata tcaaatcaag
      961 aattgacgct tactccctta agaggaaaaa gtggcaaagc ctatattggt acttatccaa
     1021 atggtgaacg tgtctttgtc aaatataata cgacacctat tttgccagcc ttagctaagg
     1081 aacagattgc gccacaacta ctgtgggcac gccgtacaag taatggcgat atgatgagtg
     1141 ctcaagaatg gttagatggt cgaacattga ctaaagaaga tatgggtagt aaacagatta
     1201 ttcatatttt actgcgcctg cacaagtctc gtcccttagt aaatcaactg ttacaattag
     1261 ggtataaaat tgaaaaccct tatgatttgt tgatggattg ggaaaaacaa actcctattc
     1321 aaatccgaga aaacacttat ttgcaatcta ttgtgactga gttaaagcgt agtttacctg
     1381 agtttcgaac agaggttgca acaatcgttc acggagatat caaacatagc aactgggtta
     1441 ttacaacaag tggtttaatt tatttggttg attgggattc tgttcgttta acagatcgca
     1501 tgtatgacgt tgcatatatt ttaagtcatt atattccaca aaaacactgg aaagattggc
     1561 tgtcttacta cggttataaa gataatgaga aagtctggag caaaattatt tggtatgggc
     1621 aattttcata tttatcacag ataatcaaat gttttgataa acgagatatg gagcatgtca
     1681 atcaagaaat ttatgaatta cgaaagttta gagagttaat taaaaagtat aatgcgagtt
     1741 agaaaacgta agggtgcaga ggaacactta gaaaataatc cgcactatgt tatttcaaat
     1801 ccagaagaag ccaaagggcg ttggcatgag atttttggaa ataataaccc cattcacata
     1861 gaagttggtt ccggaaaagg ggcttttatc actggtatgg cagagcaaaa tccagatatc
     1921 aattatattg gaattgatat tcaattgtca gtgctttcct atgctctgga taaagttcta
     1981 gatagcggtg ctaagaatat caaattacta ttagttgatg gttctagtct atcaaactac
     2041 tttgatactg gcgaagttga tttgatgtat cttaacttta gtgatccatg gcctaagaaa
     2101 aagcatgaga aacgtcgatt gacctataag actttcttgg atacctataa agacatactt
     2161 cctgagcagg gggaaattca ttttaaaaca gataatagag gtttatttga atatagcctt
     2221 gctagctttt ctcaatacgg tatgacttta aaacaagttt ggttagattt acatgcaagt
     2281 gattaccagc aaaatatcat gactgagtac gaacgcaaat tttcaaataa aggacaggtc
     2341 atctatcgtg tagaagcacg attttagaat aagtaaatag taagtgggta gttatttgct
     2401 actcctttat ggagaggtcg catagtggct gagtgcgcac gcttggaaag cgtgtagtcc
     2461 ctaatggggg ctcgggggtt cgaatcccct cctctccttt aatcttgtta atgtatcagt
     2521 gtttattaag tgttcacctt tttcactcgt caaaagtcca agtttcgaaa aatttctttt
     2581 attttatcat cgtttctctt tgagcttgaa gtattagcat aaagttttct aagctcttac
     2641 aaagaattga ggacaaattt taattttttg atataagatt agtgtacggt gatgctaatt
     2701 taaagtaaat gattgcaata atcgtatttt gtgctataat agtattaaat aatacagaaa
     2761 ggggcggagt tttacttcgc ctcttatgtg tgtgattcat agaatttata gtcattgtaa
     2821 gttctaaagc aagttagaaa gaagaggtcg ctgttatcgc aaatcaaacc attgttgata
     2881 ttgttactca agtcgtaact cctgctattc aagcaccgtt tgaattagta gatgtagaat
     2941 acgaaaaaat gggtggcgac tatgtgctca gtattttaat tgataaaccc ggaggcatta
     3001 cggttgaaga tacagcacaa ttgacagacg ttgtaagtcc cttacttgac acaattcaac
     3061 cagatccatt tccagaacag tatatgcttg aagtctcaag tcctgggttg gaaaggccac
     3121 ttaaaacagc agaagcatta tcaaatgctg ttgggtctta tatcaatgta agtctgtata
     3181 aatccattga taaagttaaa atatttgaag gtgacttatt atcatttgat ggtgaaacct
     3241 tgacaattga ttatatggac aaaacacgtc ataaaactgt agatattccc tatcagacgg
     3301 ttgcaaaagc ccgtcttgcg gttaaacttt aagaaaggat gattcttgcg aagcaagaat
     3361 ggacactatg agcaaagaaa tgctagaagc cttccgtatt ttggaagaag aaaaacacat
     3421 taataaagaa gatattattg atgcagttac tgagtcacta aaatctgcct ataagcgtcg
     3481 ttatggtcaa tcagaatcat gcgttattga atttaacgag aagaaagcag actttaccgt
     3541 ctatactgtt cgtgaagttg ttgatgaagt ttttgatagc cgtttggaaa ttagcctaaa
     3601 agacgcactt gctattagct cagcgtatga acttggtgat aagattcgct ttgaggaatc
     3661 tgttacagaa tttggacgcg tggcagctca gtcagcaaaa caaactatta tggaaaaaat
     3721 gcgtcgtcaa atgcgtgaag taaccttcaa tgaatacaaa caacacgaag gtgaaattat
     3781 gactggtact gttgaacgat ttgaccaacg ttttatctat gtcaatctcg gttcactcga
     3841 ggcgcaatta tcgcatcaag atcagattcc tggggagagc tttaaatctc acgatatgat
     3901 tgacgtttat gtctataaag tggagaataa tcctaaaggt gtcaacgttt ttgttagccg
     3961 tagtcatcca gagtttatca aacgcattat ggagcgagaa attccagaag tatttgatgg
     4021 aactgttgag attatgagcg tctctcgtga ggctggagat cgtactaaag ttgctgtacg
     4081 cagtcataat tcaaacgttg atgcgattgg aacaattgtt ggacgcggtg gaagtaacat
     4141 taaaaaagtt atcagtaatt tccatcctaa acgcgttgat gctaaaacag gattggagat
     4201 cccagttgaa gaaaacattg atgttattca atgggttgaa gatccagcgg aattcattta
     4261 caatgctatt gcaccagccg aagttgatat ggtacttttt gatgatgaag atacaaaacg
     4321 tgctactgta gttgttccag atagcaagtt atcgctagct attggtcgcc gaggacaaaa
     4381 tgtgcgttta gcagctcatt tgactggcta tcgtattgat attaaatctg caagtgaata
     4441 cgaaaaaatg gaagcgcaag aacttcaaac tgaggaagta gctcaagaat ctgaagttat
     4501 ttcagataag gcagaataat ataaatgaga gaggtgtttt atggctaaaa caaaaaaaat
     4561 acctttaaga aaatcagttg tttctggtga agtgatcgat aagcgagatc ttttacgaat
     4621 tgttaaaaat aaagaaggac aggtttttat agatcctacc ggaaagcaaa atggtcgagg
     4681 tgcctacata aaattggaca atgatgaagc tatcctagcg aaaaagaaac gagtgtttga
     4741 tcgcagtttt tcaatggaag tttctgatga attctatgat gaactattgg catatgtcga
     4801 ccataaggtc aaaagaagag agttaggcct tgaataattc agaaaaagta ttaaatctca
     4861 ttgggttagc acagcgtgcg gggcgcctta ttacaggtga agaattggtt attaaagcaa
     4921 ttcaaaatca acaagtatcg ctaatctttc tagcaaatga tgctggccca aatttgacga
     4981 aaaaagtaac tgataaaagt aattactaca aaacagaagt ctccacagtg ttttcaacac
     5041 tggaattaag tgatgcgtta ggcaaaccaa gaaaagtggt tgctgtcgca gacgctggat
     5101 tttcaaagaa aatgaggact cttatggcat aacagaatag gaggacatca attgtcaaag
     5161 aaaagattac acgaaatcgc taaggaaatt ggaaaaacga gtaaggaagt ggttgagcaa
     5221 gctcaatcgc ttggcttacc tgtaaaaagt catgcttcta gcgttgaaga aaatgatgct
     5281 acacgtattg ttgagagttt tagctcttct aaaactaaag ctccaacaaa ttctgttcaa
     5341 actaatcaag gtgtgaaaac agaatctaaa actgttgaaa ccaaacaagg cttgtccgat
     5401 gataaacctt caactcaacc tgtagctaaa ccgaaaccac aaagtcgtaa ctttaaggcg
     5461 gagcgcgagg ctcgtgctaa agctgaagcg gagaagcgtc aacataatgg ggatcaccgt
     5521 aagaataatc gccataatga tacacgctct gacgacagac gccatcaagg tcaaaagcgt
     5581 tcaaatggta atcgtaatga taaccgacaa ggtcagcaaa ataaccgtaa taaaaatgat
     5641 gggcgttatg ctgattataa acagaaaccc caaacgcgtc ctcagcaacc tgcaggcaat
     5701 cgcattgact ttaaagctcg agcagcagct ttgaaagctg agcaaaatgc tgaatactca
     5761 cgtcatagtg agcagcgttt ccgtgaagag caagaagcca aacgtcaagc agctaaggag
     5821 caagaactag ctaaagcagc agcgttaaaa gcgcaagaag aagctcaaaa agctaaggaa
     5881 aaacttgctt ctaaacctgt tgctaaggta aaagaaattg tcaataaagt agccgctacg
     5941 ccatcacaga cagcagatag tcgtcgtaaa aaacaaactc gttctgataa gtctcgtcaa
     6001 ttttcaaatg aaaatgaaga tggtcaaaag caaacaaaaa ataagaaaaa ctggaataat
     6061 caaaaccaag tgagaaatca aagaaatagt aactggaatc ataataagaa aaataaaaaa
     6121 ggtaagacta atggagctcc aaaaccagta acagagcgta aattccatga attaccaaaa
     6181 gaatttgaat atactgaggg catgaccgtt gcggaaattg caaaacgtat taaacgcgaa
     6241 ccagcagaaa ttgttaaaaa actctttatg atgggagtta tggcaacgca gaatcaatca
     6301 ttagatggag acactattga gctattgatg gttgactacg ggattgaagc acatgctaag
     6361 gttgaagttg atgaagctga tattgaacgc ttctttgcag atgaggatta ccttaatcct
     6421 gataacttaa cggaacgtcc gcctgttgtt actatcatgg gacacgttga ccatggtaaa
     6481 acaactcttc ttgatactct acgtaattca cgtgtcgcta ctggcgaagc tggtggaatt
     6541 actcaacata ttggtgctta tcagattgaa gaagctggca agaaaattac tttcttggat
     6601 acaccagggc atgcggcctt tacttctatg cgtgcgcgtg gtgcatcagt aactgatatt
     6661 actatcctta tcgttgccgc tgatgatggt gtcatgccgc agacagttga ggcaattaat
     6721 cactctaaag ctgcgggtgt cccaattatt gttgctatca acaaaattga taaacctggt
     6781 gcaaatccag aacgcgttat cagtgaatta gctgagcatg gtgtcatctc tactgcatgg
     6841 ggtggtgagt ctgaatttgt tgaaatttca gctaagtttg gtaaaaatat tcaagaatta
     6901 cttgagacag ttcttttagt tgctgaaatg gaagaattaa aagcagatgc agatgttcgt
     6961 gctattggta ctgttattga agcccgtctt gataaaggta agggagccgt tgcaacgctt
     7021 cttgtccaac aaggtacttt aaatgtacaa gatccaatcg ttgttggtaa tacatttgga
     7081 cgtgttcgtg cgatgactaa tgatcttggg cgtcgtgtca aagttgcggg tccatctaca
     7141 ccagtttcta ttactggttt gaacgaagct ccaatggctg gtgatcattt tgctgtttat
     7201 gcagatgaga aagctgcgcg tgctgcaggt gaagaacgtg caaaacgtgc tctcttaaaa
     7261 caacgccaaa acacgcaacg tgttagcctc gagaacttat ttgataccct taaagctggt
     7321 gaagttaagt cagttaatgt tattatcaaa gctgatgtgc aaggttctgt agaagcactt
     7381 gcagcttctc tccttaagat tgatgttgaa ggagtaaaag ttaatgttgt tcactcagca
     7441 gttggtgcta ttaatgagtc tgatgtaact cttgctgaag catcaaatgc tgttattata
     7501 ggatttaacg tacgccctac tccacaagct cgtcaacaag ccgatgctga tgatgtcgaa
     7561 attcgtcaac atagcattat ctataaagtt atcgaagaag ttgaagaagc gatgaaaggt
     7621 aaacttgatc ctgaatacca agaaaaaatc cttggtgaag ccattattcg tgagaccttt
     7681 aaagtttcaa aagtgggaac tattggtgga tttatggtta tcaatggtaa agttactcgt
     7741 gattcaagtg ttcgtgttat ccgtgatggt gttgtcattt ttgatggtaa attagcaagt
     7801 cttaaacact ataaagatga tgttaaagaa gtcggcaatg ctcaagaagg tggtctaatg
     7861 attgagaatt ataatgatct caaagaagat gacactattg aagcatacat tatggaagaa
     7921 attaaacgta aataacccct atgagaaacc caacttgtat ctttagttgg tagggaatac
     7981 aaaattatgg ctaggctcgt cctagctttc aagttatgag cacaatgaag aaaggattaa
     8041 ttatggctaa tcatcgtatt gatcgcgttg gaatggaaat taaacgtgaa gtcaatgaga
     8101 ttttgcgtct ccgagttaat gatccacgtg tacaagatgt cacaattact gatgttcaga
     8161 tgttgggtga cctttcaatg gctaaagtat tttatactat tcattcgaca cttgcttctg
     8221 ataatcaaaa agcacaaatt ggtttagaaa aagcaactgg tactattaag cgtgaacttg
     8281 gcaaaaacct tactatgtat aaaataccag atttacaatt tgttaaagat gaatctattg
     8341 aatacggaaa taagattgat gaaatgttgc gaaatctaga taaaaaagat taagaataat
     8401 aaaaaataaa ccatgtcaaa atcatggttt attttttata gctttagagt tgagatagct
     8461 agtattaagg agttttattg atgatatcaa tcatttgata ctgtgctgtt ttagcttctt
     8521 caatagggta aattggataa acatgattca ttccctcttg tgcaatatag ttaacggggt
     8581 gattagctgc ttggagttgt gcagcataat cgcgaatatc tgggaagaat atttctcgtg
     8641 ttccagtaaa taacgtaata ggagcgagtt tagtagcagg ggcattttta ggactaacat
     8701 atgtatggtt agtattatta gaaccatttg cccaaatttc acctacacgt gcgagacccc
     8761 aagcagatag gataggatcg gtatcttcgt atttgggaat ttctggatgt ttcattgtca
     8821 catctagcca tggagataag aggataatat ttttaggttg aggaatagcc tcttgccctg
     8881 attggtgaga caatgcatgc gctaatccta gtgctaatcc tccgccagct gaatcaccca
     8941 tcaaagtgag attagcgttt ttttcatgaa agtgtctata aaggttaacc aatctaggga
     9001 tagcataatc ataggtataa cgaggagttt ttggataaat tggtaaaata atttttgcat
     9061 caagactagt tgagagcgtc ttaagcatgc tgatatggta aggcgttgga ttatttaagt
     9121 aagagccacc agctaaatag aaaatggtct tttgattagc tttaccgttg tcattccaag
     9181 aatagacttg catatcaaaa agtctctctt gaacaagccc tccattaaca gagtttgggt
     9241 ctaagtaata acccttatca gattgttcac ggctagcatt taagtacttt tgaaatgagc
     9301 tttcatcttg aactttataa taacttttat cgcgctttag ccaaaagtat tgttcgataa
     9361 accaactttt ccatgaacgc ccacttgctt ttgcatgtgg tgtacaggcg atggataaaa
     9421 tggtgataat actaaatagt agcaatagtt ttttgaagga cggtttcaag gtattctcct
     9481 taatataatg ttatttttgt gtaatattat tataataaaa ccaacatctt tgtcaataga
     9541 ataaagaaag atgagaaatg tttttcggtt tacaaatgta gatttatttg ctaaaattgg
     9601 tttacaaacg taaatcgaaa gaggtgagaa aatgactatt tcaaacgcag aatgggaaat
     9661 tatgagagtg gtttgggcac agcagaatac aaccagtaat gagatactag cagttttact
     9721 agagaagtat gattggactc cttcaactgt caaaacctta ttaagaaggc tcttagataa
     9781 aggctacgtg agcagagaaa aaatgggaaa aggatttagc tattcttctt taattgatga
     9841 agatttagct atgatgtcag aggtagatag tgtattgcag aaagtttgcc agaccaaaca
     9901 tgttgctatt gttagacact tattggaaag tataccaatg acagagaagg atcgattaaa
     9961 tttgcaagca tcactagaag ctaagaaagg caaaacactt gaacgtgtag cttgtaattg
    10021 cattcctggt cagtgtcaat gccattagaa aggagaaaat atggctaata gaaaagaaac
    10081 atttttgatt gatgggatga cttgtgcatc ttgtgcttta acgatagaaa aagctgtcaa
    10141 caagttagac catgtggata gtgcagtagt aaatttagca acagagaaaa tgacagttac
    10201 gtttgatgat acaaccttat cgccaaacgt gattgaagag tgtgtttctg agactggata
    10261 cgaagcatcg cttttcaaag aggaaacttc taaaagccaa tctgaacggc accaattagc
    10321 aattgaaaag atgtggcatc gcttttggat gtcagcagtt gcaactatac ctcttctcta
    10381 tatttccatg gggccaatga ttaacttatg gttaccatca tttttgatgc cagataaggg
    10441 acctctaaac tatggtgtga tacaattatt gttaacacta ccggtgatgt attttgggcg
    10501 tatcttttac caaaatggtt ttaaagcact ttttaaaagg catcccaata tggattcatt
    10561 agttgctata gccacaacag cggctttcat ttatagctta tatggattgt acgaaatatt
    10621 acagggtgac atacactacg ctcaccagct ttatttcgaa tctgttgcag ttattttaac
    10681 acttattact ctaggaaaat attttgaaat attatccaaa gggcgtactt cggcctcaat
    10741 tgaaaaatta ttaactttat cagcaaaaga agcaagagtt atcaaagatg gtgaagacta
    10801 tatggttcct ttggataaag ttaaaatagg tgaaacaatt cttgtcaaac ctggagaaaa
    10861 aattcctctt gatggccatg ttgtagcagg agaatcaagt attgatgaat ccatgttaac
    10921 aggtgaaagt attccagttg aaaaaaaggt tggtagtaag gtttacggtg ccagcattaa
    10981 tgggcaaggt agcttaacga ttttcgttga aaaagaagct ggcggttccc ttctatcaca
    11041 aattattaac ttagtagaag ttgcacaggc aagtaaggct ccaattgcta acttagcaga
    11101 caaagtatca ggagtctttg tcccttttgt tattgtcatt gcaattttat caggtttgag
    11161 ttggtactta attctggggc aaagttttgc cttctctctg aagattatga ttgcagtctt
    11221 agtgattgct tgtccatgtg ctttagggtt agcaactcct acagctatta tggtggctag
    11281 tggtaaagca gctgagaatg gtattttatt taaaggtggt gaagtgttgg aaaaggctca
    11341 ccacattgat acaatagttt ttgataaaac aggaaccttg accaaaggga aaccagaagt
    11401 agttgctatc aaaacatatg gggtagataa agaggagttt ttaggtcaag tagcatcagt
    11461 agaaaaactt tcaaatcatc cacttagcca aacaattgtt aacaaagcaa aagagaaaga
    11521 acttccctta agggaagtca tggcttttaa aaatattttg ggttacggtt tgagtgcaat
    11581 tattaatggt aaaactatgc ttgtaggtaa tgctaactta atgactaaga acgatgttaa
    11641 tttagattta gcaaaggctg atatagaaat agctcaagaa gaagcacaaa ctgtggttta
    11701 cgtttccgaa aatggagtgt tgagcggttt aataacttta acggaccagc taaagacaga
    11761 tagccaagaa acagttaagc agttacaaag attaggattt aatttagttt tacttactgg
    11821 tgataataag gctagtgcag atgccatagc acaaaaattg ggtatcacga cagttgtttc
    11881 agaagtttta cctgatcaga aagcaaacgt tattttagaa cttaaagaaa aaggcggaca
    11941 gattgcgatg gttggtgatg gcatcaatga tgctcctgcg ctagctagca gtgatgtagg
    12001 tatctctatg agttctggaa cagatattgc tattgaatca gcagatatcg tgttgatgaa
    12061 acctgagtta acagatctct taaaagcaat gactatcagt aaacaaacga ttcaaattat
    12121 taaggaaaat ttattttggg ctttctttta taacgtttta gcgattccag tggcaatggg
    12181 tgttttacat ctttttggtg gtcccttact caatcctatg ttggcaggct tagctatggc
    12241 gtttagttca gtttcagtag ttttaaacgc acttcgctta aaagtcttaa aataataaag
    12301 ataataagac tgaattttta tgaagggaga agaatatgaa acatacctat cgagttagtg
    12361 gcatgaaatg tgatggttgt gccaaaacag tttctgataa gttatcaagt gtgatgggtg
    12421 ttgacgaggt taatgttgat ttgactaaaa atcaagttgt cgtaagtgga aaaacattca
    12481 aatgtctttt aaaacgttct ttaaaagaca caaagtattc actagaagag gaaataaatt
    12541 aataactaaa gaagtggtta cagcttttaa tttcttaatt tgagcagttt ctcacttctt
    12601 ttttattcta aaaatgataa actagaatag aattagttgt gaggagtgca gatgtcaatc
    12661 gatatttgga acattttaag tttggttgga accgtagctt ttgcaagttc gggagctatt
    12721 gtggcgattg aggaagaatt tgatgttcta gggcttttta tcttaggttt tgtaacggca
    12781 tttggtggtg gagctattag aaatgtacta attggcttgc ctattgaaac actttggagt
    12841 caaggaattg ctttttatgc agcagcagca gctattttgt ttattatgat tttccctaac
    12901 ttgttgagtg gtaagggacg ggatgccgaa gttgtatcag atgctattgg gttagcagcg
    12961 tttagtgttc aaggagcact atatgcgact caatctcatc aacctcttag tgctgtcatt
    13021 gtcgcagcag tactaactgg tgcaggtggt ggtattgtca gagatgttct ggctggccga
    13081 aaacctgggg tactaagaag tgaaatatat gcaggttggt caatattagt ggggatcatt
    13141 ttatatttta aaattgctaa aacaacaact gactattatt tgttagtgtt agttgtaact
    13201 agcctaagga tgttggggta taagaaacaa tggcacttgc cagttgtcag accacgtaag
    13261 aaatgataga ggagaaagag atgataaaat taattgctat tgatatggat ggaacgcttt
    13321 taaatgatga aaagaaaatt ccaaaggaaa atatccaagc tattaaagaa acaactcaag
    13381 caggtattaa aattgtactg tgtactgggc gaccgatgtc aggtatactt ccttatttta
    13441 atgaactagg tttaacaaaa gaggagtata ttatcatgaa taatggttgt agcacttata
    13501 gcacgaaaga ttggcaattg attgattctg caactttaac acatgatgaa ctcatctttc
    13561 ttgaggaggt tgttaaagaa tttcccaacg tttgtttaac gttaacagct gaaaatacat
    13621 tttacgctgt tggtgaggaa gttcctgaaa ttgttgctta tgatgctgat ttagttttta
    13681 caaaagcaaa gtcaataagt ttagatgctc ttcgcaatca agaagaaatt gtctttcaag
    13741 caatgtacat gggattagat gcagatgtaa ctgcttttca agaagctgtt gaagaagcct
    13801 taatctcgaa atttagtgga gttcgaagcc aagactatat ttatgagatt atgcctcaag
    13861 gagtaacaaa agcaagaggc ttaaaatcct tgatagcaaa attaggactg gatattaatc
    13921 aagttatggc tattggtgat gctcctaacg atattgaatt attagattta gttcccaata
    13981 gcgttgctat ggggaatgca tcagatgaga ttaaatctag atgtaaatat ataacggtgg
    14041 ataataacca agccggggtt gctaaagcta tttatgacta tgctttacgt taaaatagat
    14101 atagaataat aaaaggaact ttaaacactg tttgtttagg gttcttttgt ttaataaact
    14161 aacaaaattc agaagtttac agatagctta tgctataata gacctatgac aaacaagaat
    14221 aaattacttt taattgatgg ctcatctgta gcctttcgtg ctttttttgc tctttataat
    14281 caaattgacc gctttaaaaa caattctgga cttcatacta atgccatata tggtttccat
    14341 ttgatgttga atcatatttt aggtcgtgtc cagcctagtc atattttagt ggcttttgat
    14401 gctggaaaaa ctactttccg tacggaaatg tatgcagatt ataaaggtgg gcgtgctaaa
    14461 actccggatg aatttcgtga acaatttcct tatattcgtc aacaattaga tgtcttaggc
    14521 atcaaacatt atgaattgga acattatgaa gccgatgata tcattggtac tcttgcaaaa
    14581 caagccgaag ctagtaaaga gcattttgat ataacggtgg tttctgggga taaagattta
    14641 attcaattaa cagacactaa cacggtagta gaaatctcta aaaagggagt tgctgagttt
    14701 gaagaattca cgccagctta ccttatggaa aaaatgggaa ttacgccgag tcaatttatt
    14761 gatttaaaag ctttgatggg agataaatca gataatattc ctggggttac taaaatcggt
    14821 gaaaaaactg gcttgaagtt gctatcagaa tatggttcct tagaagggat ctatgaaaat
    14881 attgaggcta tgaaacagtc taagatgaaa gaaaacctta tcaatgataa ggagcaagct
    14941 ttcttatcaa agaccctagc tacaatcaac atagcatcac ctataacaat cggcttagaa
    15001 gatatccttt atagtggacc gcaagatata aaagccttgt ctcaatttta tgatgagatg
    15061 gattttaaac agtttaaagc agctctgggt gaggagacaa gtcaggaaga ttttgaggtt
    15121 gactttactg aagtggagca attaaaacca gaaatgttct ccgataatga tttttattac
    15181 tttgagatgt taggtgataa ctaccacgta gaagatttaa ttgggatagc ttggggaaat
    15241 agcgatacaa tctacgctac tagtgatgta tcgttattgc aagaagcttt atttaaaaaa
    15301 gctttatcaa aacctatcaa aacttatgat tttaaaagaa gtaaggtcct tttaaatcgt
    15361 tttaatattg atttaccaga gccagccttt gatactagat tggccaagta ccttttgtct
    15421 actactgagg ataatctagt atcaacaatt gctaggttat atacaaatct gccactggat
    15481 actgatgatg ctgtttatgg gaaaggtgct aaacgcgcta taccagaaaa aacacgattt
    15541 cttgagcatc tagctaaaaa agtaaaagtt ttagtggatt ctgaggctaa tgttatgcaa
    15601 cagcttaaag ctaatgagca agaagaattg ctgtttgaaa tggagcaacc tttagcgaat
    15661 gttttagcta aaatggaaat tcgaggtatt aaggttaaga aaaatacctt gaatgaaatg
    15721 gctattgaaa atcaaaaagt catcgaaacc ttaacacaag agatctatga attggctggg
    15781 caagagttta atattaattc tccaaaacag ttaggaaagt tgttgtttga aacgttaggt
    15841 cttcctgtag aaatgactaa aaaaacaaaa acaggttatt caactgcagt agatgtatta
    15901 gagcgcttag ctccgatttc tcctcttgta actaaaattt tagagtaccg tcaaatcact
    15961 aaattgcaat caacctatat tattggttta caagattata ttttagaaga tggtaaaatt
    16021 catacacgtt atgttcaaga tttgactcag acaggtcgtc tttcaagttc tgacccaaac
    16081 cttcaaaata taccagttag actagagcaa ggacggctta tccgaaaggc ttttgttcca
    16141 tcagaagaca atgctgtttt attgagttca gattattctc aaatagaatt gcgcgtgtta
    16201 gcacatattt caaaagacga acatttgatc gctgccttta aagagggagc agatattcat
    16261 acttcaacag ctatgcgtgt ttttggtatc gaaaaacctg agaatgtcac tcccaatgat
    16321 cgtcgaaatg ctaaggctgt taactttgga attgtttatg gtatttcaga ttttggtcta
    16381 tcgcataatt taggaatacc tcgtaaacta gcgaagcaat atattgacac ctattttgag
    16441 cgttatcctg gaataaaaaa ctatatggaa acagttgttc gtgaagcaaa agataagggt
    16501 tacgttgaaa cacttttcca tcgtcgtcgt tcactaccag atattaattc tcgtaatttt
    16561 aacattcgcc aatttgcaga gcgcacagca atcaattcac caatccaagg aagtgcagct
    16621 gatattctta aaattgcaat gataaattta gatagggtac ttgataaagg tggttataaa
    16681 tcaaaaatgc tgttgcaagt tcatgatgaa attgtacttg aagtccctaa cgaggagata
    16741 ggagcaatca aggagttggt tacaaaaaca atggagtcag caattagttt atcggtacct
    16801 ttgattgcag atgagaacgc aggtgagact tggtatgaag cgaagtagtg gacaacatac
    16861 gtaaaggagt cattaatatg gtttatcatt ttcaaaatct aagtgatttt atgttaaaaa
    16921 attatttaac aaaagcaaag accatcgcag ttgtaggatt gtcagaccgt caagaaactg
    16981 cagcttatca agtatcaaaa ataatgcaag aagcaggtta tcaaattatt ccagttaatc
    17041 ctaaaaatgc aggacagaaa atacttgggc aaatgacata tgcctcactt aaggatgtca
    17101 ctgaacatat tgatattgtt aatatttttc gacgtagcga gtatttacct gatattgcaa
    17161 gagaattttt agaagtagac gctgatattt tctgggctca gcttggactt gaaagtcaag
    17221 aagcagagac aattctaaag caagcaggac acaaacagat tgtcatgaat aaatgtctga
    17281 aagttgaata ccaaaaatta cactcagaaa ctaactaatt accgtgttta ataggcaaat
    17341 aatataaatt atgctataat aaccatgcta aattaaagtt cgtgctggaa aggagtccta
    17401 tggacagtca ttcacatggt catagacctc tcgatgctta tgaaaacgtc ctagagcatc
    17461 tccgtgaaaa acgtattcga attactgaga ctcgaaaagc tattatttcc tatatggtta
    17521 atagtcgtga gcatccgagt gcagaaaaga tttacaatga tttattacca gaatatccta
    17581 acatgagctt ggcaacagtt tacaataacc ttaaggtcct tgtggatgag gggtttgtga
    17641 cagagctaaa actttgcaat tatagtacaa cttattatga ttttatgggg catcagcacc
    17701 taaacattgc ctgtgaagat tgtgggaaaa tcgttgattt tgtcgatgtt gacttattgg
    17761 atatttctag agaagcacac caacaaacag gttttgaagt gactagagtt cagttagtag
    17821 cttatggtat ctgtccggag tgtcagagga agcgtaagga taaatataaa aaagattgaa
    17881 acgtaattgt tcaatctttt tttgtttcta gactaagaga aaaagtttaa gttttactaa
    17941 gtaataatta agaaataatt tataattgct taaaaaatga tttatttttt gttggtatac
    18001 tctcattaac aaaaagaaag aggaatccct tatgaaaata tcacaataca ataaatggtc
    18061 aattcgccga ttgaaagttg gagctgcatc tgtaatgatt gctagtggaa gcattgttgc
    18121 tttagggcaa tctcatattg tttcagcaga tgagatgtcc caacctaaaa caaccattac
    18181 agccccaaca gctaacactt ctacaaatgt agaaagtagt actgataagg cattatcaaa
    18241 agttactacg atggagacaa gttcagaaat gcctaaaatg caaaatatgg caaaagttga
    18301 aaaaacgagt gacaaaccta tgatggtggc tacctctgtt agaaaaatga tggcaacgcc
    18361 gacacctgta gcgatgacca aaacgactag tgtagatgag gtaaagaaat caactgatac
    18421 tgcttttaaa cagacagtag acgttccggc tcattatgtt aatgcagcaa aagggaatgg
    18481 cccatttcta gcaggtgtaa atcaaacgat tccatatgaa gcgtttggtg gagacggcat
    18541 gttaacgcgt ttaatcctta aagcttcaga gggagcaaaa tggtcagaca atggtgttga
    18601 taaaaatagt ccattattac cattgaaagg cttaactaaa ggtaaatatt tctatcaagt
    18661 ttctttaaat ggtaatacga caggtaaaga ggggcaagct ctcttagatc aaattaaagc
    18721 taatgataaa catagctacc aagccacaat tagagtttat ggtgctaaag atggtaaagt
    18781 tgatttaaaa aacatgatta gccaaaaaat ggtaaccatt aacattcctc acattacaac
    18841 tgatatggaa gtaaaaaatt ctctaaagat ggcctttaaa gaaaaagtag acgtcccagc
    18901 aaaatatgtt agtgctgcaa aagctaaagg tccattttta gcaggtgtta acgaaacgat
    18961 tccatatgaa gcgtttggtg gagatggcat gttaacgcgt ttaatcctta aagcttcaga
    19021 gggagcaaaa tggtcagaca atggtgttga taaaaatagt ccattattac cgttaaaaga
    19081 tttaactaag ggtaaatact tctaccaagt ctctcttaat ggcaatacag ccggtaaaaa
    19141 aggccaagct ctcttagatc aaattaaggc aaatggaagc catacttatc aagcaactat
    19201 tactatttat ggtacgaaag atggcaaagt tgatatgaat actattttag gacaaaagac
    19261 agtgatgatt catattaatg tcgctaaaaa agatatgaat agcacatcaa tgatgatgaa
    19321 aaaagataag atgacaatgc ctatgaagaa agaaatgact tcatctaaaa taaataccgg
    19381 tatgatgatg tctaacaaca agatgtcagc taatatgcaa atgtcatcac aagccaaatc
    19441 aaatgataaa gctggtaaaa aaatgtctat gatgtctaaa aatttaccta atacaggtga
    19501 aacaaagcaa caaaatgtag gtgtgctagg tatgcttagt ctagcctttg cgacaggtct
    19561 aactgcgcta ggccttaaaa aatctaaaca aagataggga tagtatttaa ttcagaacac
    19621 caaatttgtt taaaattatt gagggttatc gtggtaaaat agtgttatac tagaggtata
    19681 gcactatttt gactcattag gagagtagaa tgacacaaaa attattatta gttgacgatg
    19741 aatttgaaat tattgatatt aatagacgtt atctagagca agcaggatat gaagtgtcag
    19801 tagctgcgga tggcatagaa gcactcaaag aggtagatga gaatcgtttt gacctaatta
    19861 ttagtgatat tatgatgcct aaaatggatg gctatgattt tattagtgaa gttctagttc
    19921 gagaacccaa ccaacctttt ctctttataa ccgctaaagt atctgaacca gataaaattt
    19981 actctttgag tatgggtgca gatgacttta tttccaagcc tttcagtcct agggaattag
    20041 ttttacgtgt taaaaatatt ctaagacgta tctatgggaa tcatcaacaa tctgaagtat
    20101 tgacgattgg tgatttggtt atcgaccaaa aacaacgttt ggtaatggta gactgcaata
    20161 caatttcctt aaccaataaa tcatttgact tgctttggat attagcaaat catttgaatc
    20221 gtgttttttc aaaaacagag ctttatgaac gtgtctgggg cgaagaattt ttagatgaca
    20281 ctaatacttt gaatgttcat atccatgcct tacgcaatga ccttgctaaa tttagtacag
    20341 acaatacccc aactatcaaa acagtttggg gattagggta taaattagag gaataaaatg
    20401 aaattaaaat attatattgt tattggttac cttatttcta tgttaataac tgtagcaggt
    20461 gttttctttg ggcttaatca tatgctgatt gagactcgag gtgtttacta catcctttca
    20521 gttacaatta ttgcctgtat cgtaggtggt atagttaatt tgttcttatt atcaagtgta
    20581 tttacatctt tgaaaaagtt aaaacaaaaa atgaaggata tttctcaaag acgttttgat
    20641 acaaaagcac aaatttgttc accacaagaa ttcaaggatt tagagacagc ctttaaccaa
    20701 atgtcttcag agttagagtc aacgtttaaa tctctaaacg agagtgaacg cgaaaaaaca
    20761 atgatgattg cacaactttc acatgatatc aagactccaa ttacctctat acaatcaaca
    20821 gtagaaggta ttttagatgg tattatatct gaagaagaag ttaactatta
//
LOCUS       DATROY010000015        19802 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.015, whole genome shotgun sequence.
ACCESSION   DATROY010000015 DATROY010000000
VERSION     DATROY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19802
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..635
                     /locus_tag="VBD11_000542"
     CDS             <1..635
                     /locus_tag="VBD11_000542"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6953516.1"
                     /translation="LIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALRSDLAVL
                     SMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYDVIVHIF
                     MEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTTVLENES
                     LFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            646..1476
                     /locus_tag="VBD11_000543"
     CDS             646..1476
                     /locus_tag="VBD11_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6953517.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            1489..4134
                     /locus_tag="VBD11_000544"
     CDS             1489..4134
                     /locus_tag="VBD11_000544"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6953518.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            4272..4574
                     /gene="gatC"
                     /locus_tag="VBD11_000545"
     CDS             4272..4574
                     /gene="gatC"
                     /locus_tag="VBD11_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6953519.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            4574..6040
                     /gene="gatA"
                     /locus_tag="VBD11_000546"
     CDS             4574..6040
                     /gene="gatA"
                     /locus_tag="VBD11_000546"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6953520.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            6040..7482
                     /gene="gatB"
                     /locus_tag="VBD11_000547"
     CDS             6040..7482
                     /gene="gatB"
                     /locus_tag="VBD11_000547"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6953521.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            7612..8526
                     /locus_tag="VBD11_000548"
     CDS             7612..8526
                     /locus_tag="VBD11_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6953522.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            8610..9170
                     /locus_tag="VBD11_000549"
     CDS             8610..9170
                     /locus_tag="VBD11_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6953523.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            9170..10288
                     /gene="yqeH"
                     /locus_tag="VBD11_000550"
     CDS             9170..10288
                     /gene="yqeH"
                     /locus_tag="VBD11_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6953524.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            10381..10698
                     /gene="yhbY"
                     /locus_tag="VBD11_000551"
     CDS             10381..10698
                     /gene="yhbY"
                     /locus_tag="VBD11_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6953525.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            10828..11460
                     /locus_tag="VBD11_000552"
     CDS             10828..11460
                     /locus_tag="VBD11_000552"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6953526.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            11457..12044
                     /gene="yqeK"
                     /locus_tag="VBD11_000553"
     CDS             11457..12044
                     /gene="yqeK"
                     /locus_tag="VBD11_000553"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6953527.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            12107..12628
                     /locus_tag="VBD11_000554"
     CDS             12107..12628
                     /locus_tag="VBD11_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6953528.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            12630..12986
                     /gene="rsfS"
                     /locus_tag="VBD11_000555"
     CDS             12630..12986
                     /gene="rsfS"
                     /locus_tag="VBD11_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6953529.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            13052..13786
                     /locus_tag="VBD11_000556"
     CDS             13052..13786
                     /locus_tag="VBD11_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6953530.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            13809..14060
                     /locus_tag="VBD11_000557"
     CDS             13809..14060
                     /locus_tag="VBD11_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953531.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            14057..15166
                     /locus_tag="VBD11_000558"
     CDS             14057..15166
                     /locus_tag="VBD11_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6953532.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            15259..15973
                     /locus_tag="VBD11_000559"
                     /pseudo
     CDS             15259..15973
                     /locus_tag="VBD11_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            16112..16516
                     /locus_tag="VBD11_000560"
     CDS             16112..16516
                     /locus_tag="VBD11_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6953533.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(16781..17494)
                     /locus_tag="VBD11_000561"
     CDS             complement(16781..17494)
                     /locus_tag="VBD11_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6953534.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(17504..17878)
                     /locus_tag="VBD11_000562"
     CDS             complement(17504..17878)
                     /locus_tag="VBD11_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6953535.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            complement(17878..18456)
                     /gene="dhaL"
                     /locus_tag="VBD11_000563"
     CDS             complement(17878..18456)
                     /gene="dhaL"
                     /locus_tag="VBD11_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6953536.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            complement(18494..19483)
                     /gene="dhaK"
                     /locus_tag="VBD11_000564"
     CDS             complement(18494..19483)
                     /gene="dhaK"
                     /locus_tag="VBD11_000564"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6953537.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            19657..19802
                     /locus_tag="VBD11_000565"
                     /pseudo
     CDS             19657..19802
                     /locus_tag="VBD11_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
BASE COUNT         6517 a         3162 c         3996 g         6127 t
ORIGIN      
        1 atcttattca attaaatcaa aggcaagaaa gaattattga tattatcaaa cagtttcagc
       61 ccattactgg tgagctaata gctgagaaat taggagtaac aagagcagca ctaaggtctg
      121 atttggcggt tttaagtatg ctaggtatca tagatgcaaa acctaaggtt ggttattttt
      181 atttaggaca gtatcatgct tcaataggga caagtcattt tgaaaagatg acagtttcag
      241 aaattatggg gatccctctg acagttcatc aaaaagattc agtttatgat gttattgtac
      301 atatttttat ggaagatgct ggttgtgctt ttatcttgga tgatgatgat tttctctgtg
      361 gagtcgtgtc acgtaaagat ttactaaaaa ccagtattgg cggaggagat ctttctaaaa
      421 tgccaatagg aatggtgatg acacgtatgc cacacgtgac aactgtttta gaaaatgaaa
      481 gtctttttgc ggcagctgat aaattagtga gcagaaaagt ggatagtctc cctgtcgttc
      541 gtcatgataa gcaatatccc gaaaaattta aagtgatcgg gaaattatct aaaacaatcc
      601 tcgctagttt gtttttagaa ataagagatt attaggagga aactgatgga agatcaactc
      661 actatattta ttatttcaga ttctttaggg gaaacagcca aagctattgc gaaagcatgt
      721 ttgtcacaat ttccaggaca tgatgattgg cattttcaac gtttttccta tattaacagt
      781 caggaacgct tagaacaagt ttttgaagag gctagccaga agacagtatt tatgatgttt
      841 agtttagttg atgttgcttt ggcttcctat gcacagaaac gatgtgaaag tgaacattat
      901 gcttatgttg acttattaac aaatgttatt caaggaatat caaggatatc gggtatagat
      961 cctttgggag aaccagggat tttacgacgc ttagacaatg attatttcaa aagagtagaa
     1021 tcaatagaat ttgcagtaaa atatgatgat gggagagatc cgcgaggtat tttacaagca
     1081 gatttagtta ttataggaat ttcaagaact tctaaaacac cattaagtat gtttttagca
     1141 gataaaaata ttaaagttat taatattcct ttggttccag aggttcctgt tcctaaagaa
     1201 ttacgtatga ttgattctag aaggattatc ggactaacta attcggttga tcatcttaat
     1261 caagtccgaa aagttcgatt aaagtcctta ggtctttcta gtacggcaaa ttatgccagt
     1321 ttagaacgga ttttggagga gacgaggtat gctgaagaag ttatgaaaaa tttaggttgc
     1381 ccaatcatta atgtatctga taaagcaatc gaagaaacag caacgattat tttagaaatc
     1441 ttaaaaacaa atggacaagt agctaaaaat ctgtaaaagg agacgctgat ggaaactaag
     1501 tttgtgtatc attttgatga aggttgtaag gaaatgaagg aactgctagg tggtaagggg
     1561 gctaatctag ctgaaatgac gagtattggg ttaccggtac cacaaggttt tactattaca
     1621 acacaggctt gtaatgatta ttatgataac gcttgccata tccgagagag tattcttagt
     1681 caaattgatc aagcaatggc acaattggag gttgaacaga acaagcaatt aggaagtgtt
     1741 gatgatcctt tgttggtgtc agttcggtca ggatcagttt tttcaatgcc tggtatgatg
     1801 gacacagttt taaatttagg gttaaatgat cgtagtgtcc aaggtttagt gaaaaagaca
     1861 gaagacgagc gttttgctta tgacagttat cgtcgtttta tccaaatgtt tgcagatgtg
     1921 gttacaggca ttccaaaata taaatttgat accattttag atcgacttaa gacagataaa
     1981 tgttatcaag atgacacaga actaactggt tcagatttaa agagacttgt tgaattttac
     2041 aaggaactat atcaaaaaga agcaggagaa aaatttccgc aagatcctaa aaggcagctt
     2101 ttgttagcaa ttgaggcagt ttttaaatca tggaataatc caagagctaa gatttatcgt
     2161 aaattaaatg atattccgga gacgcttggg acagccgtta atatccaagc tatggttttt
     2221 gggaatatgg gaaataacag cggaacggga gttgctttta cacgaaaccc atcaactgga
     2281 gcagcaaatt tatttggtga gtatttgatt aatgcacaag gagaagatgt tgttgcaggt
     2341 atcagaacac cacaatccat atcaaaattg gcagaacaaa tgcctattat ttaccaagag
     2401 tttgtaagcg ttacccaaaa gttagaagct cattatcgcg atatgcaaga tatggagttt
     2461 acaattgaaa atggtaatct ttatatgctt cagacgagaa gcggtaagcg tacagcgaag
     2521 gcagctatca aaatagcagt agatcaagta aatgaaggcc taattagtaa agaagaagct
     2581 atactacgta tcgagcccaa acaattagat cagttattac acccttcttt tgacttgaaa
     2641 agtttaaaga aagctattat tttgacgaca ggacttccag cttctcctgg agctgcttat
     2701 gggaaagttt atttccatgc agaagatgtt gttaaagaga tgaaaaaggg aaatccagtg
     2761 ctattggtca gacaagagac atcacctgaa gatatcgagg ggatggttag tgcaaacggg
     2821 attataacag caagaggcgg aatgacttcc catgcggcag tagtagccag aggaatgggg
     2881 aaaccatgcg ttgcaggctg tagtcaatta ttggttgatg aagtaagacg agaaatttct
     2941 attgggcatc aaacgataaa agaaggtgag atgttatcaa tcgatggtgc tacaggaaat
     3001 gtttatatag gacaagttcc gatggcagag actagcgtag atcgtgattt tgagatattt
     3061 atgaaatggg tagatgaaaa tcgagatatg atggtgtgtt caaatgcaga taatccgaga
     3121 gatgctcaaa aggccttgga ctttggagca gaaggaatag gtctttgtcg cacggaacat
     3181 atgttttttg acgatgaacg tattccagtc gttagagaaa tgattttagc ggatgaaata
     3241 ttgagccgaa ggaaggctct agaaaggctt ttatctttcc aaagagacga tttttatcaa
     3301 atttttaaag tattgaaagg taaagcttgt acgattcgtt tgcttgaccc tcctttacat
     3361 gaattcttac cccatgataa agagtctata gaatctatgg caagacaaat ggggatatct
     3421 actttagcta ttgaaaaacg gattcaaaca ttagaagaat tcaaccctat gttaggtcat
     3481 cgtggttgta gacttgcgat tacctatccg gaaatttacc aaatgcaggt gagagctctt
     3541 gtacaaggcg ctatacttgc aatgaaagag ggatatgagg caaagccaga aattatgatt
     3601 cctttagtga cagcgcatga agaaatctct ataatacgtg atctgattga agagacaatt
     3661 gttgaggaga gtaaatctaa aaaaattaac ttatcattcc ctattgggac aatgattgaa
     3721 acaccaagag cttgtatgat agcggatgat attgcaaagt ttgcggattt ctttagtttt
     3781 gggaccaatg atttaacaca aatgagcttt ggattttcac gagatgatgc tggaaaattc
     3841 ttaggagaat acgttgataa aggacttcta aaaaaagacc ctttccaagt tttagatcaa
     3901 aaaggaatag gaaggtttat tgagcaggca gtgaggttag gaaaagaagt taagccaaac
     3961 cttaaaatag gtatctgtgg agagcatggt ggagaaccat catctattga gttctgttac
     4021 caacaggggt tacattatgt atcgtgttcg ccgtttagga ttcctattgc taaattagcg
     4081 gctgcacagg ctaaaatcaa acaaagcaga aatgatataa caatagataa ataaacagta
     4141 aacgcttctt atcataggta agaagcgttt attatgaaaa gctgggataa tgtgataaca
     4201 ttgagagttt aatatgctat aatagtagag actatcttaa aacgaaaatg atacagagat
     4261 aaggagaact catgaaaatt tctgaagaag aagtgcgtca tgttgcccat ctgtcaaaat
     4321 tgcgcttttc tgatcaagag actaaagaat ttgcgtcaag tttgtcaaag attgttgata
     4381 tgattgaact cttaaatgaa gttgacacag aaggcgtacc agtaacaacg accatggcag
     4441 atcgcaaaac tgtgatgcgt gaagatattg ctcaaccagg ccataatcgt gatgacttat
     4501 ttaaaaatgt accacagcat caagattatt atattaaagt acctgcaatt cttgaggatg
     4561 gaggagatgc ctaatgtcgt ttaataatca atcaattgat cagttgcatg attttctcgt
     4621 taaaaaagaa atttcagcaa ctgaattaac aaaagctaca ttggaagata ttcatgcgcg
     4681 tgaacaagcg gttggatctt ttattactat ttctgatgag atggcaatag ctcaagccaa
     4741 agaaattgat gataaaggaa ttgatgctga taatgttatg tcaggtattc ccttagcagt
     4801 taaagataat atttcaacaa aggggattct tacaacagct gcttctaaaa tgctctataa
     4861 ctatgagcca atctttgatg ctacggctgt tgaaaagtta tatgctaaag atatgattgt
     4921 gattggtaaa gcaaacatgg atgaatttgc catgggaggc tctacagaaa catcttattt
     4981 taaaaagaca aataacgctt gggaccatag taaagttccg ggaggatcat caggtggttc
     5041 tgcagcagca gtagcttcag gacaagtacg tttatcattg ggctctgata caggtggttc
     5101 aattcgccaa ccggcttcat ttaatggaat cgttggtatg aagccaactt acggacgtgt
     5161 atcacgcttt ggtttgtttg ctttcggtag ttcgcttgac cagattggac cgatgtctca
     5221 aacagtaaaa gagaatgcac agcttttgac agttatttct gggcatgatg taagagattc
     5281 aacttcttct gagcgtactg taggcgattt tactgctaaa attggtcaag atattcaagg
     5341 aatgaaaatt gctttaccaa aagaatattt gggagaagga atagcgcaag gagttaaaga
     5401 aacgattatt aaggccgcaa aacatctgga aaaactaggt gcagttattg aggaagttag
     5461 tttaccgcat tctaaatacg gtgttgctgt ttattatatt gttgcttcat cggaagcttc
     5521 ttcaaactta caacgttttg atggtattcg ttatggatac cgtacagaaa attataaaaa
     5581 cttggatgat atctatgtca atacacgatc tgaaggtttt ggagacgaag ttaagcgtcg
     5641 cattatgctg ggaacgttca gtttatcttc tgggtattac gatgcttatt ataaaaaagc
     5701 aggtcaagtt cgtagtttga ttattcaaga ttttgaaaaa gttttcgcag attatgactt
     5761 gattttaggt ccaacagctc caacaacagc ctttgactta gattctttaa atcacgatcc
     5821 agttgcaatg tacctagctg atattctaac aataccagtt aatttggctg gtttaccagg
     5881 tatttcaatc cctgctggtt ttgatcaagg tctaccggtt ggtatgcagt tgattggtcc
     5941 gaaattcagt gaagaaacaa tatatcaggt tgctgctgcc ttcgaagcaa cgacagatta
     6001 tcataaacaa caacctaaaa tttttggagg tgagaactaa tgaattttga aacagtcatt
     6061 ggacttgaag tccatgttga attaaatacg aactctaaaa ttttctcacc ttcttccgct
     6121 cattttggtc aggaacaaaa tgctaataca aatgtaattg actggtcttt cccaggtgtc
     6181 cttccagtca tgaataaagg agttattgat gcaggaatca aggcggcact tgccttgaat
     6241 atggatatcc atcaaaacat gcattttgat cgtaaaaatt atttctatcc agataatcct
     6301 aaagcttatc aaatttcaca gtttgatgag cctattggtt acaatggttg gattgaaatc
     6361 gaattagaag atggtacacg aaaaaaaatt cgtattgagc gtgcccacct cgaagaagat
     6421 gcaggaaaaa atacacatgg gacagatggt tattcctatg ttgaccttaa tagacaaggt
     6481 gttccactga ttgaaattgt atcagaagct gatatgcgtt ctcctgagga agcttatgct
     6541 tatttgacag ctctcaaaga gattatccaa tatactggaa tttctgacgt taaaatggaa
     6601 gaaggttcta tgcgtgtaga tgccaatatt tcacttcgtc cttatggtca agaagagttt
     6661 ggtactaaag cagaacttaa gaaccttaac tcattcaata atgtacgtaa aggtttgata
     6721 catgaagaaa aacgtcaagc acaagtttta cgatctggtg gtcaaattca gcaagagact
     6781 cgtcgttttg atgaaacaac aggtgagact attttgatgc gtgtcaagga aggctcatca
     6841 gattatcgct atttccctga accagatcta ccactatttg atatctcgga tgaatggatt
     6901 gatcaagttc gtttagaatt accagaattt ccacaggaac gtcgtgcgaa atatgtatcg
     6961 tcttttggtc tatcatctta tgatgctagc caattaacag ctacaaaagc aacttctgat
     7021 ttctttgaaa aagctgttgc tattgggggt gatgcaaaac aagtttccaa ttggttacaa
     7081 ggagaagtgg cgcaatttct taacagtgaa tcaaaatcaa tcgaagaaat tggtctgact
     7141 cctgagaatt tggttgagat gattggcttg attgctgacg gtacaatttc atccaaaatt
     7201 gctaaaaaag tttttgtgca tttagctaaa aatggtggtt cagctgaaga attcgttaaa
     7261 aaggctggtt tagttcaaat ttcagatcct gaagtattaa tcccaattat tcatcaagtc
     7321 tttgcagata atgaagctgc tgtgattgac tttaagtcag gaaaacgcaa tgctgataag
     7381 gccttcacag gttatctaat gaaagcaaca aaaggacaag ctaatccaca agttgctctg
     7441 aagctgttag cgcaagaatt agcaaaatta aaagaagaat agaaatcagc cttttggctg
     7501 gtttttattc gctaatttag cacgaacacc tagggataaa taattaagaa gctaagatat
     7561 ttatcagatg actttaacta cagtgtttaa gaaataaaaa tgaggggaaa aatgactaaa
     7621 aaagaaaaag gaacaatgat gaccttagct gccggactag catggggcat ttctggaatt
     7681 agtggtcaat acttgatgtc gcacggagta cacgttaacc ttttgacttc tttacgattg
     7741 ttgataacag gtatctttct cttgtcttta gctagatcaa aacaaaagga gcatttagta
     7801 gcagcgtgga aacagccaaa atttttaaag caggttttac ttttttctat atttggttta
     7861 gttttaaatc aatatgcttt tctcagagcg attcatttaa caaatgctgg aactgcaacg
     7921 gttttgcaat atatggcacc aatacttatt ttgagtattg tttgtatcct aaacagacaa
     7981 agaccaacat cttttgaaat aatagcaatt gctatggcaa ttttaggaac ctatatgatt
     8041 gcaacgcatg ggaagttagg aagtttagct atcacaccga aaggattaat gtgggggctt
     8101 ggttccgcca ttacttattc tatttatatt ttattacctg tgaaacttat tcatgaatgg
     8161 ggaagtacga ttgttattgg ttcaggaatg tttataggtg ggattttatt tagtttggtg
     8221 acaaaagcgt ggcaataccc cttacaaatt aatgtgatga gtatacttgc ttatattggc
     8281 attattggta ttgggactat ttttgcttac acttttttcc ttaaaggggt tagtattgta
     8341 ggtgctgtca agggtagctt attagcatcg gtagagccag tttcttcggt atttttgaca
     8401 gtacttgttt taggagaaat tttttatcca atagatttac ttgggatgct gtttattttt
     8461 cttgctgtaa ctctaatctc ttacaaagat ttaattgcat tgaagaaaat gaaacgagta
     8521 agatagtatc ttttggggtt gaaaccctaa tttaaaggtc tttaaagacc ttttttcttt
     8581 actcgctatc tcttaagaat tatggtagaa tgaatagtaa aatatatttt ttggagatga
     8641 aattgagtat agatgattat aggccgacct tcgtggttga ggcagtttat gatttaagag
     8701 cagaagattt attacgtcat ggcattcgtg cggttttagt tgatttagat aatacactca
     8761 ttgcttggaa taatccagat ggtactgctg aggtgcgtgc atggttagat gagatgacaa
     8821 cagcggatat ttcagttgta gtagtgtcta ataacaatca tgctcgcgta gaacgtgcag
     8881 tatcacgttt tggtgtggat tttgtaagtc gtgcgatgaa accatttact cgtggtataa
     8941 acatggcaat tgagcgttat ggctttgatc gtgacgaggt gattatggtt ggagatcaat
     9001 tgatgacaga tattcgtgct agttatcgcg caggaataaa gtccgtattg gtaaaaccaa
     9061 ttgttaaatc tgatgcttgg aataccaagt tcaatcgctt acgtgaacgt cgcgtttgga
     9121 aaaaaataga agagaattac ggtaaaatcg tatatcaaaa aggaatttaa tggaagagtt
     9181 attttgtatt ggctgcggag ccaggattca aactgaaaat aaagatgctg ctggctatac
     9241 acctcgtgca gcacttgaaa aaggtttaga gacaggagaa ttatactgtc agcgttgttt
     9301 cagattgcgc cactataatg agattactga cgttcatatc actgatgatg aatttttgaa
     9361 attactacat gaggttggag atagtgatgc ccttgtggtg aatgtcattg atatttttga
     9421 tttcaatggt tcaattattc ctggtcttag tcgtttcgtt gcaggaaacg atgttctttt
     9481 agtaggaaat aaaaaagata ttcttcccaa atcggttaaa gatggaaaag taacacaatg
     9541 gttaactgag agagctcatg aagaggggct tcgtcctgtt gatgtcatct tgacaagtgc
     9601 ccaaaatcat catgctatta aggatttgat tgatacgatt gaaaagtatc gtcatggtcg
     9661 agatgtttat gttgttgggg ttactaatgt tggaaagtca actcttatta acgctattat
     9721 tcgtgaaatt actggtagtc gtgatgttat tacgacctca cgtttcccag gtacaacgtt
     9781 agataaaata gaaatacctc ttgatgatgg tagctatatt tttgataccc cgggtattat
     9841 tcatcgtcat caaatggctc attatttgac agcaaaaaat cttaaatatg ttagtcctaa
     9901 aaaagaaata aaacccaaaa cttatcagct taattcagaa caaacattat tcttggctgg
     9961 cctagcacgt tttgatttta tttctggtca aaaacaaggt tttacggcat attttgataa
    10021 taacctgaat ttacaccgca ctaaactagt tggagcagat gaattttaca ctaaacatgt
    10081 tggaaaatta ttgacaccac caacaggtaa ggaagtttca gattttccta aattagttcg
    10141 tcatgaattt acaatcaaag ataaaatgga tattgtttat tcgggtcttg gttggatacg
    10201 tgtgaaatca gaagcagaaa atccagtagt agtagctgct tgggctccag aaggcgtggc
    10261 agttgtttta cgcaaagctt taatttaata actaaattaa ccagcaacat atgacggttt
    10321 ttcatctaat ttagctgatg atacagtcat aatcctataa tagataaaaa ggaaactaat
    10381 atgttaacat ctaaacaacg tgctttctta aaatcagaag cacattctat gaaacccatt
    10441 attcaaattg ggaaaaatgg tttaaatgat caaatcaaaa caagcgtacg taatgcactc
    10501 gatgcacgtg agctaatcaa agtaaccctt ttacaaaata ccgatgaaga tatccacgat
    10561 gtcgctgaag ttctggaaga tgaaattggc tgcgatactg tccttaaaat aggacgtatc
    10621 ttgattcttt ataaagagtc agcgcgcaaa gaaaatcgta aaatttcagt taaagtaaaa
    10681 gcagtaaaat caaactaaac tacaatagat taagaaatag agtatagaac taaaaaacga
    10741 tttacctaag ttacacttcc gtatgatagc tgatcatgat aatcagtgtt tgttttttta
    10801 gagatactac ttgttcatgg aggaattatg gcactcgaat tgctaacacc atttacaaaa
    10861 gttgaattag aagagaaaaa gcgagatact aaccgaaaac aaattggaat tatgggagga
    10921 aattttaacc ctgttcataa cgcccacctt gttgttgcag atcaagtacg tcagcaattg
    10981 tgtttagacc aagttctgtt gatgcctgaa tttcagccac cacatattga taaaaaagag
    11041 acaattgatg aacagcatcg tctcaagatg cttgaattag caatagaagg cattgatggt
    11101 ttatctattg aaccaattga aattgaacgt aaatgtatca gctatacata cgatacgatg
    11161 aagcttctaa ttgaaaaaaa tccagatgtt gattattatt tcattattgg ggcagatatg
    11221 gtggaatatc tacctaagtg gcatcgtatt gatgaacttg ttaaaatggt gcagtttgtt
    11281 ggtgttcaac gtccaaaata taaagctggt acatcatatc ccgttatttg ggtggatctg
    11341 ccattgatgg atatctcatc aagtatgatt cgtcaattta ttaaaagtaa tcgtcagccc
    11401 aactatttac tgcctagaga ggtattagat tatatcagga aagaaggtct ttacaaatga
    11461 cctacaaaga ttacacaggt ttagatcgga ctgaactttt gagtaaagtg cgtcatatga
    11521 tgtccgacaa acgttttaat catgtgttag gagtagagcg tgctgcaata gagttagcag
    11581 aacggtatgg ttatgacaag gaaaaagcgg gtctagctgc tttattacat gattatgcta
    11641 aggaactatc tgatgatgaa tttcttagat taattgataa ataccaacta gatcccgacc
    11701 taaaaaaatg gggtaataat atttggcatg gtcttgttgg catttataaa atacaagagg
    11761 atctagctat caaagatcaa gatattttag ctgctattgc aaaacatact gttggttcag
    11821 cccaaatgtc cacattggat aaaatcgttt atgtagcaga ttacattgaa cacaatcgag
    11881 attttccagg agttgaagaa gctcgtgagt tagcgaaagt tgaccttaat aaagcagtag
    11941 cttatgagac tgctagaaca gttgcgtttt tagcaagtaa ggctcagcct atctatccta
    12001 aaacgattga aacctataat gcttatatac cttatctcga ctaattcttt tattgaataa
    12061 tggtcatgat aggtttggcg atgcagtggt cattttggag gaaagtatga cttgtgcact
    12121 tttactaatt gatatccaac aaggtattat ggataagaaa ccaaagcatt taacaaattt
    12181 tgcagagctt ttagatgatt tacttttaag tgccaaaggt tctaattgtg aagtgatatg
    12241 gataaggcat catgataagg aattgcctca aggttctcca cagtgggaaa tttgggaaca
    12301 gcgacatctt gtgacgcatc acaaaataat tgataaaact tacaatagtt gttttaaaga
    12361 tactcattta catgattact tgcaaagtaa gcatattagt caattaatca tgatgggact
    12421 tcaaacagaa tactgttttg atacatcagt aaaagttgct tttgaatatg gttatgatat
    12481 ctttattcca caaggtggac acctaacatt tgatacacca actttaagtg gggattctat
    12541 taaaaaacat tacgagaata tttggcatca tcgttttgcg acaatggtag caaaagatag
    12601 cttattgaaa ttaataaacg aggaataaaa tgacagaaaa agacttacta cagctagtcg
    12661 ttaaggctgc ggatgaaaaa cgagcagaag atattgtaat tttagattta cagcctgtaa
    12721 cgagtgttgc agattacttt gtgattatga gtgcaagtaa tagtaggcag ttagaagcaa
    12781 tcgcagacaa tatccgtgaa caagtaaaag gaaatggtgg ggatgctagc caccttgaag
    12841 gcgatagtaa agctggttgg gtacttcttg acctcaatag cgtcgtggtt catatcttct
    12901 ctgaagatga gagacagcac tataaccttg aaaaactttg gcatgaagca ccattgcttg
    12961 atgctgaagt ctttatgaca gaataaagct aagcagtctt gggactgctt tttgagagat
    13021 gtaatatcaa gaaagatttg agaactatta tatggcaacg tatgaaactt ttgcagctgt
    13081 ttatgatgct gtcatggatg atactttata tgataaatgg actgattttt cacttcgtca
    13141 ttttccaaaa ggtaagaaaa aacttttgga attagcatgt ggtacgggaa ttcagtctgt
    13201 tcgatttgca caagctggtt atgctgttac aggacttgat cttagtggcg atatgctgaa
    13261 acttgccaaa aaacgtgcaa cttctgctca ccaaagtatc cagtttattg aaggaaatat
    13321 gttagattta tctaatgttg gtaaatatga tctcatcacg tgctattcag actctatttg
    13381 ttatatgcaa gatgaagtcg aggttggtga tgtttttatc gaagtctata aggccttaga
    13441 agaaaatggc gtctttattt ttgatgttca ttcgacttat caaacagata aagtatttcc
    13501 tggttattct tatcatgaaa atgcagatga ctttgctatg gtgtgggata cttatgaaga
    13561 tgatgcacct cattctattg tccatgaatt aacatttttt gttcaagaag aagatggtcg
    13621 atttacacgt catgatgaag ttcatgaaga gcgtacttat gatattttga cttatgatat
    13681 tttacttgaa caggcaggtt ttaaagatgt taaggtgtat gcagattttg aagataaaaa
    13741 accaactgca acttctgctc gctggttttt tgtagctcat aaataagtaa taatttggag
    13801 gtaaaaagat gataatattc ttatacgctt gtctgatgat atttgggata ctttatatta
    13861 ttgaacgtcg ggtaagtcag aaaaagtcta aagttatttt ggaattattg atgaacccta
    13921 aacttcattt ggatttatta tcggcattaa aaccatttga aaaagaaaca gatcgtctca
    13981 tataccttcg taaaacttat cacctagata ttattgatgc tgttacttta aataatcaat
    14041 tgaaagagaa agaagtatga cagttacagg aattgttgcc gaatttaatc cctttcataa
    14101 tgggcataag taccttttag aacaagctca aggcataaaa gtaatagcca tgtcgggtaa
    14161 ttttatgcaa cgtggtgagc cggcgattgt agataaatgg acccgtgccc agatggcact
    14221 agaaaatggt gctgatttag tgattgaact cccttttctg gtttcggtac agtcagcgga
    14281 ttatttcgct agtggagctg tgtctatcct ggcacgatta ggtgtggata acttatgctt
    14341 cggtaccgaa gaaatgcttg attatgcccg tattggggat atttatgtga ataaaaaaga
    14401 ggagatggaa gcttttttga aaaaacaatc ggattctctt tcctatccac aaaaaatgca
    14461 agctatgtgg caagaatttg cagggattac attttcggga caaaccccaa atcacatttt
    14521 aggcttagcc tacaccaaag cagctagcca aaatggtatt cgattaaatc cgatacaacg
    14581 tcaaggagct ggttatcatt catcagaaaa aacagagata tttgcttctg caacaagtct
    14641 tcgtaaacat caatctgatc gtttctttgt agaaaaaggt atgcctaaca gtgacttgtt
    14701 tttgaatagt ccacaagtag tttggcaaga ttattttagt ttactcaaat atcagataat
    14761 gacgcattct gatttgacac aaatttatca agtgaatgaa gagatagcca atcgtataaa
    14821 aagtcaaatt cgttatgtgg agactgtgga tgagttagtt gataaagtcg caactaaacg
    14881 gtataccaaa gcaagaattc gacgtttatt aacctatatc ttgataaatg ctgtggaaag
    14941 tcctatacca aatgctattc atgtcttagg ttttacacaa aaaggtcaac agcatttaaa
    15001 gtctgtgaag aagtctgtgg atatcgtgac gaggattggt tcacaaacgt gggatagttt
    15061 gacgcaacga gcagattctg tttatcagat gggaaatgct aatatagcag agcaaacatg
    15121 gggacgtatc cctttttatc aacctgttag tcaaagtgac ctttgattta agctttataa
    15181 attaatcatc acaaaataat atttttccct tgacccttac ttaacgttat acattatact
    15241 ctttacagag aggttattat gaaaacagtt aaagaaataa gtcacatttc tggtattagt
    15301 gtgcgcacac ttcattatta tgatgaaata gatttgttat ctccttcatt tgtaggagag
    15361 aatggttatc gttactatga tgatgaatca ctgattaagt tacaagaaat tttgttattt
    15421 aaggaactgg aatttcctct taaaaaaata aaagagataa tggaaagtcc aaattatgat
    15481 cgtaatcaag cacttcttga tcaaattagg tggctggaac taaagaaaca gcgactagaa
    15541 gaagtaattg aacatgcaaa gtctatccag aggggtaaaa atatgtcaga ttttacagct
    15601 tataatcaag aagaattaga agtatttcaa gaggaagcta gaacacgctg ggggggatac
    15661 agatagttac aaagaatttg aaaatagcca tagtaagaat gatttttcaa tgatttcaca
    15721 agctatgagt cagattttta aagactttgg acagttgaaa gaactttctc cgacagatga
    15781 gaaagttcaa aaacaagttc agattttaca agattatatc acagctcagt tttacaactg
    15841 cactaacgat ttattagcaa gtcttgaaat aatgtacatc taagatgagc gtttccaaaa
    15901 gtctattgat aattggggag gccagggaac agcattattt gtttctaaag ctatagatag
    15961 ctattgtcat taggctatat tttaggtttg caatgaaatg ataggaatca aggaacagta
    16021 gatattatta aaaatgtcag actcttatct aaatttttga cagtaaggag caaaaagagt
    16081 agactgtagg catagaaagg taggtatcgt tatgaaagga tttcatggga atatcgaaaa
    16141 attgacacta ggaaatacaa atttccgcca agtcctctat accgcagaac attgtcaact
    16201 cgttttaatg acactgcctg ttggtggaga aattggtagc gagattcatg cagaaaatga
    16261 tcagtttttc cgttttgaag ctggtcatgg taaggttgtc attgatggta atgaatatga
    16321 agttgcagat ggagacgcta taatagttcc ggcaggtgct gaacataacg taattaacac
    16381 ttctgaaact gagatgttaa aactctatac aatttacagt ccagctcatc acaaagatgg
    16441 tattatccgt gccacaagag aagaagctga agaaaatgaa gaagattttg atggagttac
    16501 tactgaaact aaataatact agactaaaaa gcgctatgtc aatagcgctt tttagcttaa
    16561 tagtagtcat ttcttggaga atataaatag taatttagga agtaatgtta ataaccgagc
    16621 ttttaaggaa acgttaagta agggcggtta tactataaac atcctaaact tttttcataa
    16681 aatctccaga aagaacacta agtattttgg tgttttttct attttgaaag catactgcaa
    16741 ctatgaaaaa ccagaaagta ttaactttct ggttttttag ttaaactaaa gtcaaagcaa
    16801 tattgtataa gatagcccca aggacaccgc cgataattgg acctacaact ggaatccaag
    16861 cgtatgacca atctgagtta cccttatttt caataggtaa gatagcatgc ataagacgag
    16921 gccctaggtc acgtgcaggg ttaatagcat aaccagttgt tggaccaaga gagaatccta
    16981 ctgcgaaaat aactatacca acgataattg gatcaagacc tgctgcaact ttactaggac
    17041 caattgccat aattgtaatc acaagaatag cagtaccgat aatttcacca aggagattcg
    17101 atggtgtatg gcggatagca ggtccggttg agaagctggc aaggataagt ccactatctt
    17161 ttgtttcttt ccaatgagga taatagtgta accataaaat agttgccgca accattgctc
    17221 ctaacatctg tgcaatgata aaggggataa cttgtcccca agaaattgct ccaatagaag
    17281 ccattgctaa tgtgactgct ggatttagat gagcgggact gaataatcca gaaacataaa
    17341 cagcaacggt acaagcaatt ccccagccaa aaacaatagc agtccatcca gttccttctt
    17401 ctttggtttt actgagcaca tttcctgcaa ctacgccatc cccaagaaga accaaaataa
    17461 atgttcctaa aaattcccca agaaattgag tcataatatc tccttattta ttgatagtta
    17521 attcagctaa ttgtgattga atactatcca ggtcagcacc tgcctgtaga agagcagcag
    17581 cggtgtaagc tccctccact actgggacag agttaatgat aatatttttt tctgaaaaat
    17641 cagctactaa ctctaaattc atttttgctg aacctaaatc aaagaaagct aataacgtct
    17701 ttttatcatt ttgctctaca atctgttgaa cttgatcaaa agaagtacca atctcaccat
    17761 cttctgttcc acccacgtaa gttattgaga catcctttgc tacctctgaa ataagatcaa
    17821 caactccttg agcaatattt ttagagtgag aaacaacaat aataccaata tcagacatta
    17881 gacacctcca acttctagta atgctttaaa taataatcct gaagaaaatg atcctggatc
    17941 aatgtgtcct actgagcgtt ctccaacata agaagcgcgt ccttttgttg ctttcatccc
    18001 ttttgtagca tctactaagg aatcgatacg gtcttcagtc aattcatttt tttcaatcgc
    18061 ttcaataaca ccgtgccaaa catcaaccat tgttttttca ttaagttcag ctttaccacg
    18121 tttttgaatc atttcaagtc ctgcgccaag tgcctctgaa atggtacttt cactctgttc
    18181 agcttttgta attcccataa atgcagaacc atagagtggt ccagatgctc cgcctacttt
    18241 gcttaaaagt tgcatggaca cggttttgaa aacatcggca gcactactaa attcattgtt
    18301 atcaaggttt tcaataacag cagtcatacc acgcgccata ttgccaccat ggtcgccatc
    18361 acctattgga gtatctagtt cggataaata gtccttgttg gattgaattt tttggttaaa
    18421 agtgtgcatc cattctattg cagtttttac ttccataaga gttccttcct tttgttctaa
    18481 ttaacaggaa tgattaccaa gcaatggttg taacgtcact attaagtgct tctaaccact
    18541 cttgattttc cagtttaatc aaggttagtg ataaaccagc catatcaatt gaagtcatat
    18601 agttaccaag ttttttgtaa ttcacttcaa tgcctttatc ctcaaggagt ttggcaacat
    18661 cgttagcaaa gacatattgt tccataagtg gggttgcacc cataccgtta ataaggacac
    18721 catatttttc gccagattta gcatcgaacg attcaattaa cttatcaact aattctgtcg
    18781 ctaaagcctt tgaaggttgc attttttctt tacgataacc tggttcacca tgaataccga
    18841 taccaaattc aatttcatct tctgcaagaa caaatcctgg cttgcctact tcaggaacag
    18901 tagccccgct tagtgcaagt ccaacagtgt gaatgtttgg tactaattca tcagcaatag
    18961 ctttgatttc ctgaagtgat ttgccatgac gagcagcatg tcctagaatt ttatggacga
    19021 gtatcgtacc agctacacca cgttttcctt gtgtgtaaag actgtcttcg actgcaatat
    19081 catcatcaac gaccacactt gcaacttcta tgccttccat ttcagccata tcttgtgcca
    19141 tttcaaagtt catgatatcc cctgaataat ttttgataac catgaaaact ccagccccct
    19201 catcagcttc tttaattgct tcaagaactt ggtcaggagt tggagaagta aatactgcac
    19261 cacacacagc tgcagataac attccttcgc ctacgaaacc agcatgtgaa ggctcatgtc
    19321 cacttcctcc gccagaaatg agagccactt ttcctgactt ctcttcattg cgagcaatga
    19381 tatcaaaacc ttctatgcga tgtactaaat cattatgaac atatgctaat ccatctagca
    19441 tttcagtaac aacatcagtt ggttgattga gaattttttt catttggaaa ctccttttat
    19501 gtttaattct cttttattat aaaatttgta agccttttct ggaatggaca gattttcttg
    19561 tttgtccgct ttgttagggt tttcaataat cataaatcat gttacactaa accataataa
    19621 atcatcagca attcaatata aaataaagga gaaattatga cttcttcgct tattactaag
    19681 aaaaaaatag ccaagtcatt taaacgttta tttatttcaa gcttttgaca aaatatcggt
    19741 gagtgatatt atggaagatg caggtattag acgtcaaaca ttttataatc actttgttga
    19801 ta
//
LOCUS       DATROY010000016        19706 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.016, whole genome shotgun sequence.
ACCESSION   DATROY010000016 DATROY010000000
VERSION     DATROY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19706
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..271)
                     /gene="cadX"
                     /locus_tag="VBD11_000566"
     CDS             complement(<1..271)
                     /gene="cadX"
                     /locus_tag="VBD11_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO6953538.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRR"
     gene            complement(283..909)
                     /locus_tag="VBD11_000567"
     CDS             complement(283..909)
                     /locus_tag="VBD11_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO6953539.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            complement(1189..1338)
                     /gene="rpmG"
                     /locus_tag="VBD11_000568"
     CDS             complement(1189..1338)
                     /gene="rpmG"
                     /locus_tag="VBD11_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6953540.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(1354..1536)
                     /gene="rpmF"
                     /locus_tag="VBD11_000569"
     CDS             complement(1354..1536)
                     /gene="rpmF"
                     /locus_tag="VBD11_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6953541.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            1756..3036
                     /locus_tag="VBD11_000570"
     CDS             1756..3036
                     /locus_tag="VBD11_000570"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6953542.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            3129..4880
                     /gene="aspS"
                     /locus_tag="VBD11_000571"
     CDS             3129..4880
                     /gene="aspS"
                     /locus_tag="VBD11_000571"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6953543.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            4870..5814
                     /locus_tag="VBD11_000572"
     CDS             4870..5814
                     /locus_tag="VBD11_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6953544.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            5922..6794
                     /locus_tag="VBD11_000573"
     CDS             5922..6794
                     /locus_tag="VBD11_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6953545.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESASATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(6821..7129)
                     /locus_tag="VBD11_000574"
     CDS             complement(6821..7129)
                     /locus_tag="VBD11_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6953546.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(7217..8908)
                     /locus_tag="VBD11_000575"
     CDS             complement(7217..8908)
                     /locus_tag="VBD11_000575"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6953547.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGSYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            9130..9567
                     /gene="argR"
                     /locus_tag="VBD11_000576"
     CDS             9130..9567
                     /gene="argR"
                     /locus_tag="VBD11_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6953548.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            9624..12200
                     /gene="mutS"
                     /locus_tag="VBD11_000577"
     CDS             9624..12200
                     /gene="mutS"
                     /locus_tag="VBD11_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6953549.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(12257..12460)
                     /locus_tag="VBD11_000578"
     CDS             complement(12257..12460)
                     /locus_tag="VBD11_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6953550.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            12686..14659
                     /gene="mutL"
                     /locus_tag="VBD11_000579"
     CDS             12686..14659
                     /gene="mutL"
                     /locus_tag="VBD11_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6953551.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            14691..15923
                     /locus_tag="VBD11_000580"
     CDS             14691..15923
                     /locus_tag="VBD11_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6953552.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            15925..16515
                     /gene="ruvA"
                     /locus_tag="VBD11_000581"
     CDS             15925..16515
                     /gene="ruvA"
                     /locus_tag="VBD11_000581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6953553.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            16538..17089
                     /locus_tag="VBD11_000582"
     CDS             16538..17089
                     /locus_tag="VBD11_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6953554.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSDNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            17178..18437
                     /locus_tag="VBD11_000583"
     CDS             17178..18437
                     /locus_tag="VBD11_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO6953555.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            18511..19650
                     /gene="recA"
                     /locus_tag="VBD11_000584"
     CDS             18511..19650
                     /gene="recA"
                     /locus_tag="VBD11_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6953556.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
BASE COUNT         6376 a         3112 c         3981 g         6237 t
ORIGIN      
        1 ctcttctagt gtccaagatg ttttgattgg ctagtttacg aagatggtgg gaggcagatg
       61 ccatactgag atttaataaa caggctatat cgcagacaca gagttcttcg acggcaagga
      121 gataaaagat gatatttatc tgtttattat cggtaaattt tgataaaatg cgaagtgatt
      181 tttggacttt ttccttttca aggtagttcg ttgcggttgt aacattttgt tgatttataa
      241 catccacttg acagatacta tcttttttca taattttttc tcctagccta acacagccca
      301 tagcatgtca aagctgttgt tttcaatcag gatatacatc cccaatccta aatagacaac
      361 ggcaataaac catctgctat atttttccaa agtttctcca acagaaggga cttgtgctaa
      421 tttttgtgca gaaaaaacca agagataaat catgactaga aaagtaagta aagtcactat
      481 caaattcgct aaatttaagg tggtaaaata tgggacaaag acaccaatat tgtcagcgcc
      541 acaacttgca aaagtaatca tagcgactag aaaaatcagg tttttattgt cttttcgcaa
      601 accatctttt gcaatagctt ctccatcaga atctcctaaa agcaaaactt ttaggcctag
      661 gaaaattgga atcaaaccga gtaaacctaa aatctcttta ctaggaatat aatttaagac
      721 aaatgcaaaa agcaaactta ggaatattag actaacagag cctagaaatt gtcctaaata
      781 gatgttaatg atgtcttttc tgctttttct tttggcaaaa aataacatta ggataataag
      841 taagtctacg gctgtcccag aatacaggat tattgaagta acaacatttt gaatcataaa
      901 atacctcatt caaatatatt tttgaatgta ttctaacatt aaactttgta gatgtcaact
      961 taaaatccac caaaatatag ataagaagtt agtgtacaaa tattaaaaag ccctgccatc
     1021 gaaatgatag cagggcttaa cttcaatatc cagatgatat atttatctaa aaaaggtaga
     1081 gtaaaaggta gagtttttat tgatttgtag tgcgatataa tgagtttcaa aaaatcaaaa
     1141 atcgcttaaa atcaatattt tgacgtctat tgatgtgtac tgaaaccctt acttaacctc
     1201 agtgaaaaca acgtgtttac gaagttttgg tgagtatttt ttcaattgaa gacggtctgg
     1261 agtgttacgt ttgttttttg aagtaaggta caagcgttca ccagattctt tatgttcaag
     1321 tgtaatattt acgcgcatgg tatctccctt ctattattta gcttcgttag ctttagcgat
     1381 tttacgtcct ttgtagtatc ctttgagtga tacacggtgt gaacgtgagt aatctccagt
     1441 agtttcgtcg aattgtacag atggagctgt caatttgtag tgtgtacgac gtttgttttt
     1501 cttcgctttt gaagtgtgac gtgcaggtac tgccattttc ttattcttcc tttcgatgtg
     1561 taagttttga ttttcatcaa cttcactatg ataacatatc ttttttgtaa agtaaagtta
     1621 cttgacaaaa ctttttattt ttacaatttc atggtgaaaa tactatcaaa ctaaagcaaa
     1681 tgatatatgc ttgtttaaaa tataagaaaa aatggtagaa tgtttaaaga gaaaactaat
     1741 cgaagaggta aacttatgaa acttcaaaaa ccaaaaggga ctcaggatat cttacctgga
     1801 gaatcagcaa agtggcaata cgttgaaaat gttatacgca atctctttaa acaatatcat
     1861 tatgatgaaa ttcgtacacc aatgtttgaa cattacgagg ttattagtcg ctcagttggg
     1921 gatacaacgg atatcgttac aaaagagatg tatgattttc atgataaagg agatcgtcac
     1981 attacgcttc gtccagaagg aacagcacct gttgtacgtt cttatgttga aaataaacta
     2041 tttgcaccag aagttcaaaa accaaccaaa atgtattata ttggatcaat gttccgatat
     2101 gagcgcccac aggctggtcg tttacgagaa tttcatcaag taggcgttga gtgctttgga
     2161 tcaaataatc caacaactga tgttgaaaca atcgcaatgg gacatcatct atttgaagat
     2221 ttaggtatca aaaacgttaa acttcatctt aatagtttag gaagtccaga aagtcgtcaa
     2281 gcctatcgcc aagctcttat tgattactta acacccatac gtgaacaatt gtctaaggat
     2341 agtcaacgtc gtttgaatga aaatccattg cgcgtactag actctaagga gccagaagac
     2401 aaactagcag ttgaaaatgc tccttctatt ttagattatt tagatgagtc aagccaagcc
     2461 cactttgatg ctgtgtgtca tatgcttgat gctctaaata ttccatacat cattgatact
     2521 aatatggttc gtggtcttga ttattataac catactattt ttgagtttat cactgagatt
     2581 gaagataatg aattaacgat ctgtgctgga ggccgttatg acggcttagt atcctatttt
     2641 ggtggaccag aaacaccagc atttggtttt ggattaggat tagaacgctt gctcttaata
     2701 ttagataagc aagggatttc tttaccaata gaaaatacta tagatctgta tatagctgta
     2761 ttaggatcag aggctaatct agctgcctta gatttggcac aatcaatacg tcatcaaggt
     2821 tttaaagtag agcgtgatta tttgggtcgt aaaatcaaag cacaatttaa gtctgcagat
     2881 acttttaatg ctaaagtgat tatgacatta ggtagcagtg aagtagattc gaaagaggtc
     2941 ggtttaaaaa ataatcaaac acgtcaagaa gttaaagtta gttttgaaaa cattaaaaca
     3001 gatttttcat ctgtgcttaa acaattagga ttgtaaaaca gttcctaaaa actctcctta
     3061 atgggtgggg tttttaggtg ctttttgtta taataggttg gaatgaatat tttaaatgga
     3121 gaaaatagat gaaacgtagt atgtatgctg ggcgtgttcg tagtgagcac ataggaacat
     3181 cgattacttt aaaaggatgg gttggtcgtc gtcgtgacct tggaggcctt atttttattg
     3241 atcttcgcga tcgcgaggga atcatgcaat tagttattaa tcctgaagaa gtagcagcct
     3301 cagttatggc aaccgcagaa agtctccgta gtgaatttgt tatagaggtt agcggtgtag
     3361 tgacagcacg agaacaagct aatgataatt taccaacagg agaagttgaa ttaaaagtgc
     3421 aagaattatc ggttcttaat acctcgaaaa caacaccatt tgaaattaaa gatggtattg
     3481 aggcaaatga tgatacacgc atgcgttacc gttacttgga tcttcgtcgt ccggaaatgc
     3541 ttgaaaattt caaattacgt gcgaaagtaa cacactcaat ccgtaattac cttgacaact
     3601 tagagtttat tgatgtcgaa actccgatgc taacaaaatc aactccagag ggggcacgag
     3661 actatttggt tccatcacgt gtcaaccaag gacattttta tgcccttcca caaagccctc
     3721 aaattacaaa acagctattg atgaatgcag gatttgatcg ttattatcaa attgtcaaat
     3781 gttttcgtga tgaggattta cgtggagatc gtcaacctga gtttacacaa gttgacttag
     3841 agacatcatt ccttagtgat caagaaattc aagacattgt tgagggtatg attgctaaag
     3901 taatgaaaga cactaaaggt ttagaggttt ctttaccatt cccaaggatg gcatatgatg
     3961 atgcaatgaa taactacggg tctgataagc ctgatacaag gtttgatatg cttttacaag
     4021 acttgacaga aattgtaaaa gaagttgact tcaaggtgtt ctcagaagct tcagtagtta
     4081 aagcgattgt tgtcaaagac aaggcggaca agtactctcg aaaaaatatt gataaattaa
     4141 cagaaattgc aaaacaatac ggtgccaaag gtttagcatg gctaaaatat gtagataata
     4201 ctatttcagg tccggttgct aagttcttga cagctattga aggtaggttg acagaagctt
     4261 tgcagctaga gaataatgac ttaattctat ttgtagcaga tagtcttgaa gtagctaacg
     4321 agactttagg agcgcttcgt acacgcattg caaaagaact tgaactcatt gattattcta
     4381 aatttaattt cctatgggtt gttgattggc caatgtttga gtggtcagaa gaagagggac
     4441 gatatatgtc ggctcatcat ccattcacct tgccaacagc ggagacagcg catgagttgg
     4501 aaggggattt ggctaaagta cgtgcagttg cctatgatat tgttttgaat ggatatgagt
     4561 taggcggtgg tagcctacgt attaaccaaa aagatacaca agagcgtatg ttcaaggctt
     4621 taggatttag cgctgaatca gctcaggaac agtttggttt cttacttgaa gctatggatt
     4681 acggcttccc accacatggt ggattggcaa taggccttga ccgctttgtt atgttgttag
     4741 caggcaagga caatattcga gaagttattg cattccctaa aaacaataag gctagtgatc
     4801 caatgactca agctcctagt ctcgtttcag aacaacaatt agaagaatta tcacttactg
     4861 tagaaagtta tgaaaactag acgaacccct ttagaaaaaa aggtaaaata tattatcagt
     4921 gtatgggcta aaaaatttgg tctcttacac actttaaaaa gtatctcacg agaaaaatat
     4981 gcagagaaaa tttcggcttc attactgtat ggtattctat ccagtgttgc tgttaatttc
     5041 ttttttcaac ctggacatgt ttattcaagt ggagcaactg gtttagctca ggttatttca
     5101 gctgtcagta aacactggtt ttcttttgaa ataccagttg ctttagcttt ttatgccatt
     5161 aatatccctt tgctgatatt atcttggcgt aagattggac ataaatttac tatttttacg
     5221 tttatcacag taactgtaag ctctatcttc attcaactaa tgcctcaaat cacccttaca
     5281 actgatccct taatcaatgc tatctttggt ggcttaatta tgggagctgg tgttggtttt
     5341 agctttaagt ctagaatttc tagtggggga acagatatta tcagtttgac aattagaaag
     5401 aaaacaggca gagatgtagg tagtatttct tttattatta atggtattat tttattattt
     5461 gcaggtcttt tatttggttg gaagtacgct ttgtactcga tggtgacaat ttttgtatca
     5521 agtagagtta cagatgccat ttttacaaag caaaaaaaga tgcaagcgat gattgttacc
     5581 agtaagcctg attgcgtcat taaacgtatc catcgtgatt tacatcgtgg tgtaacctgt
     5641 atcaatgatg ctgaaggtac ttataatcat gagaaaaaag cagttttaat aactatatta
     5701 acacgtgaag aatttagtga ttttaaatat ttaatgctaa aagcagatcc aaaagccttt
     5761 gtttccgttg cagaaaatgt tcatattatt ggacgattcg ttgatgatga ttaactgtat
     5821 atatgacttt tggtcaaccg ccactaagga aacattatga tgttattaag ctaagttgtt
     5881 taaacaactt tagctttttt gaataaggaa ggattaagcc tatgttgaaa ctggatttaa
     5941 aaacaaagat aaaagaagct attttaattg cctttggtgt ggctctctat actttcggct
     6001 ttgtcaaatt taatatggct aatcatttgg ctgaaggagg tatttccggt gtcactttga
     6061 ttatccatgc acttttcgga gttaatccag cactttcatc acttctcctt aatatcccgt
     6121 tatttatatt aggggctagg attttaggga aaaaatcctt attattaact atatatggta
     6181 cagtgttaat gtcttttttc atgtggtttt ggcaacaaat ccctgtaaca gtccctttga
     6241 aaaacgatat gatgttagtt gcagtagctg ctggaatttt agcaggaact ggtagtggtt
     6301 tagtatttag atatggtgca actacaggtg gtgcagatat tattggtcgt attgtagagg
     6361 aaaaatctgg aataaaatta gggcaaacct tattgtttat tgatgctata gttctaacgt
     6421 cttcacttgt ttatattaat ttacaacaga tgctatatac gttagtagct agctttgtat
     6481 ttagtcaagt gttaacaaat gttgaaaatg ggggctacac tgttcgtgga atgatcatta
     6541 ttaccaaaga atcagaatct gcatcagcaa ccattttgca tgaaattaat aggggtgtaa
     6601 cttttttacg gggccaaggt gcctactcag gtagagagca tgatgtcttg tacgtagctc
     6661 ttaacccaag tgaagtgcgt gatgtgaaag aaatcatggc tgatttagac ccagatgctt
     6721 ttatttctgt tataaatgta gatgaagtta ttagctcaga ttttaaaatt cgacgaagaa
     6781 attacgataa ataaaaatac aggctgtctg gcctgtattt ttattttggt cctccactta
     6841 agaaactacc aagcataata gctgagatag cagttccctt gtaatggcta gcttttgttt
     6901 ttaccaagcg actaagatca aatatgcttt taggagcatc aaattgatgt gttaagacgt
     6961 attgattgat aagtgagtca atttttgtag ctaggagtga gttcatcccc tctttttcaa
     7021 gttgactagc gagctcgaaa atagcttgac gaaaataatc atctgcttgt attacttctt
     7081 cgctataaac tttactaaga gcatctaata tttctttttc tgatggcatg gcattctcct
     7141 gtaacttatt gtgctattat tataatataa aaaattaaga ctgtcataaa tttgacagtc
     7201 ttagtaatgt catttcttac atttcattcg gagcatcaac tcctaggagg cgaagagatt
     7261 cttttaatac tgtagcagtt gcatagcaaa gtgctagacg actactgatt tcagcatctt
     7321 catctaaaat acgtgtgtgg gcgtaatatt tattgaagca ctgagctaaa ttgatagcga
     7381 attttgcaat aatagagggc tcaaaattat ctgctgcacg cacaataata cgaggaaaat
     7441 cttggatgag tttgatgatt tcccagcttt caacatcgtt gagactgtaa ttatcgctat
     7501 tagatgggtt aaagttagct ttacgtaaga ttgattgaat acgagcatgt gcatattgta
     7561 catatggccc agtttcacct tcaaatgata ccattgcttc taagtcaaag tcataaccat
     7621 ttgtacgatc agttttcaaa tcgtaaaatt taatagcacc tacaccaact gcttgagcaa
     7681 ctttatcttt atctgctagg ttagggtttt tagcttcaat ctgagatgcc gcacgattaa
     7741 tagcttctgc aacagtaggt tcaaggagaa ttacatttcc tttacgagtt gataattttg
     7801 ctcctccttt tgttacaagg ccaaaaggaa catgagtcat atcatcggac caatcgtagt
     7861 ccatttcttt aagaacagct tttagttgtt taaagtgtgc agactgttcg tttcctacaa
     7921 cgtagattga ttttgcaaaa tcgtaggtac gtttacggta aagtgcagca gctaagtcac
     7981 gtgtaatgta gagagttgct ccatcggatt ttttaatgag ggcaggatgt tcaataccgt
     8041 atttttctag gttaacaact tgtgcacctt tagattcgac taggaggttt ttagattcta
     8101 gcagttcaag cacttcatcc attttgtcgt tgtagaatgc ctcgccgtta tagctatcaa
     8161 atgtaacatt cattttatcg taaagacggt tgaattctaa taatgactcg tcacggaacc
     8221 attgccataa ttcagttgct tcaggatcgt tagcctcaag tttacgaaac cactcacgtg
     8281 cctcttcatc aacgctaggg tctatctcgg cttcagcatt aatacggaca taaagtttta
     8341 aaagttcatc aatgggatga gcacggacag cctcttcatt gccccatttt ttataggcaa
     8401 cgattaacat tccaaattgt ttaccccagt cgccaaggtg attaatttta acaggatgat
     8461 agccaatttt atcaaaaata ttagctaaac tatcaccaat aactgttgaa cgaaggtgtc
     8521 caattgagaa tggcttagca atatttggac tagacatatc aatggcaaca tttcgtcctt
     8581 caccaatatt ttgagtagca taggcagaac catcagataa gacttgtttt aaaacttgac
     8641 ttgagattgc ggctttatca aggaaaaagt taacgtaaga tccaacagct tccacctttt
     8701 caaattggtc gcttttaatt tgttcagcaa tgtcactagc aattatttga ggtgctttgc
     8761 gtagagtttt agccaaagag aatgctggga aagctaaatc tcccatgctc gaattttttg
     8821 gggtttctaa taaagaaaga atggccgatt gttccatata tggaacaact ttttgaattt
     8881 cactcgcaat tagatgtttt gtatccatgg tatctcctat tttaattagt aatatagtca
     8941 ccctatttta acataattat gaaaaataga cgagaacttg ttgattaaat tcataatgca
     9001 ttctgtaaag aactagccta ctttatagct tttttacaga atgattacga tatcactact
     9061 aagtgaaaaa gacaattttt tttgatataa tttggtaagc ataattctaa ctaaaaagga
     9121 ttgaataaca tgaataaaat agaacgccaa aaacgtatta aacgtttaat ccaatcaggg
     9181 cagattggca cacaagaaga aattaaatta catttaaaaa atgaaggaat cgatgtcacg
     9241 caagcgactc tatcaagaga cttgcgtgag attggtcttt taaaattgcg tagcccagaa
     9301 ggtaagctat attatagttt atcaacagct acttcaaatc gatttagccc agccttacgc
     9361 tcctatattt tgaaagtatc gcgagcaagt tttatgttag ttttaaatac taatttaggg
     9421 gaggctagcg ttttagcaaa ctttattgat gagaaagggc tgccagagat tttaggaaca
     9481 atggcaggag cagacacctt gttggtgatt tgtcaaaatg aagatattgc caaagttttt
     9541 gaaaaagaac tatcagtagg tttataaata taatgattga ggagctgagc atggcagcca
     9601 gccccgttag ttaggagtga gaaatggcca agccaacgat atcaccggga atgcaacagt
     9661 atctggatat aaaagagaat tatccagatg cttttttgct ttttagaatg ggtgattttt
     9721 atgaattatt ttatgatgat gcggtaaaag cagcacaaat cctggaaatt agcttgacta
     9781 gtcgaaataa gaacgcagaa aagccaatcc caatggcagg agttccctat cactcagctc
     9841 aacagtatat tgacgtttta gttgaattag gttacaaagt agccattgct gagcagatgg
     9901 aagatcctaa aaaagctgtg ggagtggtca agcgtgaggt agtgcaagtt gttaccccag
     9961 gaacggttgt ggagtcaacg aagccggata gtgctaataa tttcttagta gcgattgatt
    10021 cgcaagatca acaaacattt ggtctagcat atatggatgt ctcaactgga gagtttcagg
    10081 caaccctttt aacagatttt gagtccgtcc gtagtgaaat actaaattta aaagctcgtg
    10141 agattgtagt aggatatcaa ttgacggacg aaaaaaatca cctactgacg aagcagatga
    10201 acttgctttt atcatacgaa gacgaacgac ttaatgatat tcatttgatt gatgagcagt
    10261 taactgattt ggaaatatct gctgcggaaa aacttttaca atatgtgcat agaacacaaa
    10321 agcgtgaact tagtcattta cagaaagtag ttcattatga aataaaggac tatttacaaa
    10381 tgtcatatgc aacgaaaaat agtctagatt tactggagaa tgctagaaca agcaagaagc
    10441 acggaagtct ttactggttg ttagatgaga ctaaaacagc gatgggaact cgaatgctga
    10501 gaacttggat tgacaggcct ttggtaagta tgaatcgaat caaggaacgt caagatatta
    10561 ttcaagtgtt tcttgattat ttttttgaga gaaacgatct cacagaaagt ttaaagggtg
    10621 tatatgatat tgaacgccta gcaagtcgag tatctttcgg aaaagccaac cctaaagatc
    10681 tattgcaact cggacagacc ttatcacaaa ttcctcggat taaaatgatt ttacagtcct
    10741 tcaatcaacc tgagcttgac atcattgtca acaaaattga cactatgcct gaattagaaa
    10801 gtttaattaa tacggcgata gccccagaag cacaggctac tatcactgag ggaaacatta
    10861 tcaagtctgg atttgataag caattggata attatcgaac agtgatgcgt gaaggtacag
    10921 gttggattgc tgatattgaa gctaaggaaa gagcagcaag tggtatcggt acccttaaaa
    10981 ttgattataa taaaaaagac ggctattact tccatgtgac caattccaat ttatcactag
    11041 taccggagca ttttttccgt aaagcgacat taaaaaattc tgaacgctat ggaacagcag
    11101 aactagccaa aattgaaggt gaaatgctcg aagctcgcga gcaatcttca aatttagaat
    11161 atgatatttt tatgcgtgtt cgtgctcaag tagaatctta tattaaacgt cttcaagagt
    11221 tagcaaagac tattgcaacc gttgatgttc tacagagttt ggcagtagtt gcagaaaatc
    11281 atcactatgt tcgtcccaaa tttaatgatg aacatcagat taagattaag aatgggcgtc
    11341 atgcaaccgt tgaaaaagtg atgggagtgc aagaatatat tcccaatagt atctattttg
    11401 atagtcagac agatatccag ttgattacag gaccaaatat gagtggtaag tcgacctata
    11461 tgcgccagtt agctctgaca gttattatgg cacaaatggg aggttttgta tcggcagacg
    11521 aagttgattt gcctgtattt gatgcaatat ttactaggat tggtgctgct gatgacttaa
    11581 tttctgggca atcaaccttt atggtagaaa tgatggaagc gaatcaagct gtaaaacgag
    11641 ccagtgataa atctttgatt ctttttgatg aattaggtcg agggacagcc acttatgatg
    11701 gtatggcatt agctcaatcg attatagaat atattcatga ccgtgttagg gcaaaaacaa
    11761 tgtttgcaac tcattaccat gagttgacag atttatctga acaattgaca aggcttgtca
    11821 atgtacacgt ggctacttta gagagagatg gagaagttac cttcttacat aaaattgaac
    11881 ctggacctgc ggacaagtct tatgggatac acgtcgcaaa aatagctggt ttaccaattg
    11941 acttattgga tagggcaact gatattttat cacagttgga agctgatgca gtacagttga
    12001 tcgtatcagc cccccaagaa gctgttactg ctgacttaaa tgaggaacta gatcctggga
    12061 agcaacaagg acaattatcg ctttttgaag aacattcaaa tgcaggtagg gttattgagg
    12121 agttagaagc gatagatata atgaatctaa ctccaatgca agctatgaat gctatatttg
    12181 atttaaagaa attattataa aatagccttt aggcaacaaa aaagagacta aataattcat
    12241 ttaggctctt ttttaattat ttaacaagat tgatatttgt tgcttgtggt ccacgttgac
    12301 catcttgaat atcaaatgtt actttttgtc cttcttcaag agttttgaag ccatcaactt
    12361 taatctctga aaaatgagcg aagacgtctg ttccagtttc gctagaaata aagccaaaac
    12421 ctttttctga gttaaaccat ttcactgtac cttgtgtcat ttgtttatta cctttctaaa
    12481 aatgttactg taaaaaataa aaaaggtaaa aatgttaaac aaaaatacaa cttcaaaact
    12541 tattacgctc cctaagtcta acaggtaata ggagtgatgt caactaaaaa ttgtgttatt
    12601 gtcaaattaa tattttgaat atggtataat ggctaagata tcacaaaaaa agttacaaaa
    12661 aatcataaaa aaaggaggga tgaacttgtc taaaattatt gaattaccgg atatattagc
    12721 taaccagatt gctgctggtg aggttgttga aaggcctagt agcgtcgtta aagaattagt
    12781 tgaaaatgcc atagatgcag gaagtagcca aattaccata gaggttgaag aatctggtct
    12841 taaaaagatt caaataacgg ataacggtga agggatgact agtgaggatg ccgttctcag
    12901 tttacgacgt cacgctacat ccaaaatcaa aagccagagt gacctttttc gtattcggac
    12961 cttaggtttt cgtggtgaag ctctaccatc aatcgcttcg attagtttaa tgacaataaa
    13021 aacagctaca gagcaaggga aacaagggac gttacttgtt gctaaagggg gaaacattga
    13081 aaaacaggaa gttgtttcaa gtcctcgtgg aacaaaaatt ttagttgaaa atttattctt
    13141 taatacgcct gccagattga aatacatgaa gagtttacag tctgaactag ctcatattat
    13201 tgatattgtt aatagactca gtctagcgca tccggaagtt gcttttacac tgataaatga
    13261 tggtaaagaa atgactaaaa ctagtgggac tggcgatttg agacaggcga ttgcaggtat
    13321 ttatggttta aatactgcaa aaaaaatgat tgagatttca aatgcagatt tagattttga
    13381 aatttcgggt tatgtaagtt tgcctgaatt gacacgagct aaccgaaact atattaccct
    13441 attaattaac ggtcgttaca ttaaaaattt tcttttaaac cgctcaattc tagatggtta
    13501 tggttcaaag ttgatggttg gaagatttcc aattgctgtc attgatattc agatagatcc
    13561 ttatctagca gatgtcaacg ttcatccgac taagcaagag gtgagaattt ctaaggaacg
    13621 agaattaatg agtttaataa gctcagcaat ttctgagagt ctgaaacaat atgatttgat
    13681 tccggatgct ttagagaatt tagcgaaaac tagtacccga agtgtagata agcctataca
    13741 aacaagcttc tcactaaaac agtctggttt gtattacgat aggactaaaa atgacttttt
    13801 tatagatgca gatactgtat ctgaacctat tgctaacttt acaaaccttg acaaaagtga
    13861 cggttcagtt gacaatgatg taaaaaactc tgttaatcaa ggagcaacgc agtctcctaa
    13921 tataaagtat gctagtagag atcaagctga tagtgagaac ttcattcatt ctcaggacta
    13981 ccttagtagt aaacagtcac ttaacaaact tgttgaaaaa ttagattcgg aagaaagttc
    14041 gacttttcca gagctcgaat tttttggtca aatgcatggg acctacttat ttgctcaagg
    14101 gaatggtggg ttgtatatta ttgatcaaca tgcagctcaa gagcgtgtaa aatacgagta
    14161 ttaccgtgaa aagataggtg aggttgataa tagtcttcaa caattgttag taccatttct
    14221 atttgaattt tcaagttcag attttcttca attacaagaa aaaatgtcat tattacaaga
    14281 tgttggtatt ttcttggaac cgtatggtaa taatactttc attttaaggg aacatccaat
    14341 ttggatgaaa gaagaagagg tagagtctgg cgtttatgaa atgtgtgata tgttgttgct
    14401 cactaatgaa gtatcagtga aaaaatatcg cgcagagttg gctattatga tgtcttgcaa
    14461 gcgatctatt aaagcaaacc atacgctaga cgattactca gcacgtcatt tattagatca
    14521 gttagcgcaa tgtaaaaatc cttataattg ccctcatgga agacctgttt tagttaattt
    14581 tacaaaagct gatatggaga aaatgtttaa acgaatccaa gaaaatcata caagcctcag
    14641 ggatttagga aaatattgat aattacttgt tagggaagaa gggagaaaaa gtgaaagagt
    14701 tttttgcttt gccaaaacag ctacagttaa gagaattatt gcgttttatt tccattacgg
    14761 ttggttcagc tatttttcct tttatggcaa tgtattatgt tcaatatttt ggaaatctag
    14821 ttacaggaat tttgatcatc ataactcaac tatcagggtt tgtagcgacc ttatatggag
    14881 gccatttgtc agatgctata gggaggaaaa aagttgtgat tgtaggttca ctgctggcaa
    14941 ccattggttg ggctataact atagctgcaa atgttccaaa tcatattacg ccacacttga
    15001 cctttgtagg tatccttatc attgaaatag ctcaccaatt ttattcccct gcctatgaag
    15061 ctatgaccat tgatttgacg aacgaacaga atcgtcgctt tgtttacact ataggttatt
    15121 ggttggttaa catcgctgta atgctaggtt ctgggattgc tgggattttc tatgatcacc
    15181 atttttttga attattaata gttcttctta ttatttcagc gatttgttgt ttggtggtct
    15241 acttcaagtt tgatgagaca aaaccacagg agggaacctt caagcatgat aagggagttt
    15301 taggaacttt taaaaattat agtcaggttt tagtggataa agcgtttgtt gtttatactt
    15361 taggagcaat tggttccagt gttgtctggc tccaggttga taattatttc tcggtcaatc
    15421 ttaagcaaaa ttttgaagta gtgtctgtgt taggacatac aatcacaggt gctaaaatgc
    15481 ttagtttagc agtctttacg aacactctat taattgtctt actaatgaca actataaata
    15541 aattcataga gaattggcct ttgaaacgtc aactaatatt aggaagcttg atttgtggtt
    15601 ttggaatgct ttttaatatt tctctaaaca cttttggagc tattttgatt gccatgacgt
    15661 ttttcacctt tggcgaaatg atttatgttc cagctagtca ggttctaagg gcggagatga
    15721 tggtggaggg aaaaattgga tcatattctg gatttttagc tatcgcacaa cctgttgcaa
    15781 gtgtattagc aggtgccatg gtttcgctca gctattttac aggaaaaata ggtgttcaaa
    15841 taacattgac catttttatg ttagcaggtc tggttttaat cttatacgct accaaaatga
    15901 aaaatattga gataggaaaa tagaatgtac gattatatta aaggaaaatt gagtaaaata
    15961 acggctaaat ttattgttgt ggaaacagct ggattgggat acgtgatata tgtggcaaat
    16021 ccctactctt tttcaggtta cgttaatcaa gaagttacca tctacttaca tcaagtcatt
    16081 cgcgatgatg cacatctttt atttggtttt catacggaaa atgaaaaaga aatttttctt
    16141 aatttaattt cagtatcagg aattggtcca acgaccgctt tagcaattat tgcagtcgat
    16201 gacaatgaag gtctcgtaag tgccattgat aatagtgata ttaagtattt gactaaattt
    16261 ccaaagatag gaaaaaaaac agcacaacag atgatccttg atttgtcagg gaagtttgta
    16321 gaagcttctg gtgagagtgc gactagtcgt aaggttagct ctgagcagaa tagcaatctt
    16381 gaagaagcta tggaagcttt gttagcgttg gggtataagg cgactgaatt gaaaaaagta
    16441 aaagcctttt ttgaaggtac gaacgatact gtggagcaat acatcaaatc tagtttgaag
    16501 atgttgatga aataatttag aagtaatgag gtttcacatg aaacgatgtt cttgggtgaa
    16561 tttagataat ccattatatg tagcttacca tgataaagag tgggggagag ctgttcatga
    16621 cgatcatgta ctctttgagt tactatgttt agaaacttat caatcgggtt tatcttggga
    16681 aacagtatta aataaacgcc aggagttcag acaagttttt catcactata atattgaaaa
    16741 ggtagctgca atgtcagatg cggatttaga gataatctta caaaatccta gggttattcg
    16801 tcatcgtcta aaacttttct caacacgaca aaatgctagg tcaattatat taattcagaa
    16861 agaattcgga agttttgatc gctccatttg gtcttttgta gataataaag tacaggttaa
    16921 ctcagataac aactacaacg atgtgcctgc aagtactacc ttatccgaac gcttatctaa
    16981 ggatttaaaa aaacgcggtt ttaaatttgt tggtccaact tgcctctatt cttttattca
    17041 ggccgcgggt atggttaatg accatgaaaa tatatgtgat ttcaagtaat atcaatgtga
    17101 tatattttaa gtagcacttc tgctactttt tttgctaaaa tgtagtcatt atttcgaata
    17161 gataagaggg tgaagttgtg agggctgaaa ttatcgctgt aggaacagaa attttaacag
    17221 gacagatcgt taatacaaac gcacaatttt tgtcggagaa aatggcagaa ttgggaattg
    17281 atatttattt ccagacagct gtaggagata atgaggaacg tttattatcg cttttagata
    17341 tagctagtca gcgcagtcaa atggttatcc tatgtggtgg acttggacct acagaggatg
    17401 atttaacgaa acaaactttg gctaaatttt taggtaaatc attaacagtg gatttattag
    17461 ctagtcaaaa attggatcgt ttttttgcaa gtcgtcctca gtttgctaga acgccaaata
    17521 atgaacgaca agctcaattg gttgaaggct caatacctct acaaaacctt actggtctag
    17581 ctgttggtgg tatcgttaca tctaagggag tccaatatat ggttttacca ggtccgccta
    17641 gtgaattaaa gccaatggtt atggagcagg tggtacctat tttgtcaaat aatggcacaa
    17701 aattatattc acgtgtattg agattctttg gtatcggtga gagtcagtta gtcacaatct
    17761 tagaagacat aattaagaat cagacagatc caactatagc accatatgca aagattggtg
    17821 aggtaacttt aaggttatca acgaaagctg aaaatcagaa tgaagccgac tttaaattag
    17881 atagcttaga aaaagaaata cttgctctta agacattaga taaccgtaaa ttaaaagatt
    17941 tattatatgg ctatggtgat aataatagta tggcacgtac tgttttagaa ttattgaaag
    18001 tacaaaataa aacgattact gccgcagaga gcttaacagc agggttattt caatctcagt
    18061 tagcagaatt ttctggtgct tcgcaagttt ttaatggtgg ctttacgacc tatagtatgg
    18121 aagcaaaatc acaacttctc ggcatcccta agaaaaaatt gcaagaatat ggagttgtca
    18181 gtcattttac agctgaggca atggctcagc aagctcgcca gcttttaaaa gcagattttg
    18241 gtatcggttt aacgggagtt gctggtcctg atgagttgga aggataccct gcaggtacag
    18301 tttttattgg gattgcaacc cctgaaggcg tttcttctat taaagtatcg attggaggga
    18361 agagccgctc agatgtccgt catatcagta ctttacatgc ttttgacctg gtgcgtaggg
    18421 ctttattaaa gatataatat ttgttataat tagtagaacg atttctaaat ttgaatcaac
    18481 ctagattcat agaaatataa ggagaagaca ttggctaaaa aaacgaaaaa agcagaagaa
    18541 attactaaga aattcggtga tgaacgtcga aaagccttag atgatgcttt gaaaaatatt
    18601 gaaaaagatt ttggtaaggg cgcagtgatg cgcttaggtg agcgagctga acaaaaggtt
    18661 caagtaatga gttcaggttc tcttgcttta gatatcgctt taggagcagg tggatatcct
    18721 aaaggacgta tcgtcgaaat ttatggacca gagtcatctg gtaaaacaac ggttgccctt
    18781 catgccgtag ctcaagccca aaaggaaggt gggattgctg cctttattga tgctgagcac
    18841 gctcttgacc cagcctatgc tgcggctctt ggagttaata ttgatgagtt actcttgtct
    18901 caacctgact caggtgagca aggtcttgaa attgcaggta aattgattga ttcaggcgca
    18961 gttgatttag ttgttgtcga ttcagttgca gcccttgttc ctcgtgctga gattgatggt
    19021 gatattggtg acagccacgt tggtttgcaa gctcgtatga tgagtcaagc aatgcgtaaa
    19081 ttgtcagcat ccattaacaa gacaaagaca atcgcaattt ttatcaacca gctaagggaa
    19141 aaagtaggcg ttatgtttgg taaccctgaa acaacacctg gtggtcgtgc tttgaaattt
    19201 tattcatcgg ttcgtctaga tgttcgtggt aatactcaaa tcaaggggac tggtgagcat
    19261 aaagatcata atgttggtaa agagacaaaa atcaaagttg ttaaaaataa agttgctcca
    19321 ccattccgtg aagcttttgt cgaaattatg tatggtgaag gtatttctcg cacgggtgag
    19381 ttaattaaaa ttgctagtga tttggatatt atccaaaaag caggtgcatg gtactcatat
    19441 aatggtgaga aaattggaca aggttctgaa aatgccaaaa aatatttagc agataatcca
    19501 gcaatttttg atgaaattga ccataaagtg cgcgtgcact ttggaatgac tgaagatgat
    19561 tcgccagttc agtctgagct cgttgaagaa aaaaatgaag ctgatgatct tgttttagat
    19621 ttggacaatg ctattgaaat tgaggaataa aatagcaact atacatcttg aacttgaata
    19681 aatgtaacta aaataagttc tgacag
//
LOCUS       DATROY010000017        19481 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.017, whole genome shotgun sequence.
ACCESSION   DATROY010000017 DATROY010000000
VERSION     DATROY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19481
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            133..1215
                     /gene="hrcA"
                     /locus_tag="VBD11_000585"
     CDS             133..1215
                     /gene="hrcA"
                     /locus_tag="VBD11_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6953557.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKESEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            1218..1790
                     /gene="grpE"
                     /locus_tag="VBD11_000586"
     CDS             1218..1790
                     /gene="grpE"
                     /locus_tag="VBD11_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6953558.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            1971..3800
                     /gene="dnaK"
                     /locus_tag="VBD11_000587"
     CDS             1971..3800
                     /gene="dnaK"
                     /locus_tag="VBD11_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6953559.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            4089..5204
                     /gene="dnaJ"
                     /locus_tag="VBD11_000588"
     CDS             4089..5204
                     /gene="dnaJ"
                     /locus_tag="VBD11_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6953560.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            5322..6569
                     /locus_tag="VBD11_000589"
     CDS             5322..6569
                     /locus_tag="VBD11_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6953561.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            6640..7416
                     /gene="truA"
                     /locus_tag="VBD11_000590"
     CDS             6640..7416
                     /gene="truA"
                     /locus_tag="VBD11_000590"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6953562.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            7379..8137
                     /locus_tag="VBD11_000591"
     CDS             7379..8137
                     /locus_tag="VBD11_000591"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6953563.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            8147..8611
                     /locus_tag="VBD11_000592"
     CDS             8147..8611
                     /locus_tag="VBD11_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6953564.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            8608..9177
                     /locus_tag="VBD11_000593"
     CDS             8608..9177
                     /locus_tag="VBD11_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6953565.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(9214..10056)
                     /locus_tag="VBD11_000594"
     CDS             complement(9214..10056)
                     /locus_tag="VBD11_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6953566.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            10213..11496
                     /locus_tag="VBD11_000595"
     CDS             10213..11496
                     /locus_tag="VBD11_000595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6953567.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            11685..12251
                     /locus_tag="VBD11_000596"
     CDS             11685..12251
                     /locus_tag="VBD11_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6953568.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEV"
     gene            12524..14128
                     /locus_tag="VBD11_000597"
     CDS             12524..14128
                     /locus_tag="VBD11_000597"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6953569.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            14237..15163
                     /locus_tag="VBD11_000598"
     CDS             14237..15163
                     /locus_tag="VBD11_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6953570.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            15210..15295
                     /locus_tag="VBD11_000599"
     tRNA            15210..15295
                     /locus_tag="VBD11_000599"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15244..15246,aa:Leu,seq:aag)
     gene            15358..15804
                     /locus_tag="VBD11_000600"
     CDS             15358..15804
                     /locus_tag="VBD11_000600"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6953571.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            15966..17330
                     /gene="radA"
                     /locus_tag="VBD11_000601"
     CDS             15966..17330
                     /gene="radA"
                     /locus_tag="VBD11_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6953572.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            17466..17963
                     /locus_tag="VBD11_000602"
     CDS             17466..17963
                     /locus_tag="VBD11_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6953573.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            18117..19436
                     /locus_tag="VBD11_000603"
     CDS             18117..19436
                     /locus_tag="VBD11_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6953574.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
BASE COUNT         6390 a         3029 c         4117 g         5945 t
ORIGIN      
        1 tcattgattt gtatgattta acaagatatg ataagtgata ttagcactct ttgaataaga
       61 gtgctaattt ttgatgtttt ttccttgact atcttttaaa aaggagtata ataaaatcat
      121 agttagcagt cgatgtgtaa gagtgctaat ttcagaaaga ccgagagggg tgatgttttt
      181 gtgattacac aacgtcaaaa tgatattttg aatttaatcg ttgagttatt tacacaaact
      241 catgaaccag tagggtctaa agctttacag agaacgattg attctagtag cgccactatt
      301 cgtaatgata tggcaaaact agagaagttg gggttattgg aaaaagcaca cacttctagt
      361 ggacggatgc cgagtccagc tggttttaag tattttgtag agcactccct tagactagat
      421 agtattgatg agcaagatat ttatcatgtt ataaaagctt ttgactttga agcattcaaa
      481 ttagaagaca tgcttcaaaa agctagtcat attttatcgg agatgacggg ctatacatcc
      541 gttattttag atgtggagcc agctcgtcaa cgtttgacag ggtttgatgt tgttcagctt
      601 agcaaccatg atgctctagc agtcatgact ttagatgaat ctaagccagt gacagtgcag
      661 tttgctattc caagaaactt tttgacacgt gacttgatag catttaaagc tatcgtagag
      721 gagcgcttgc tggatggtag tgtcatggat attcattata agttgaggac agaaattcct
      781 cagatcgttc agaagtattt tgtaactacg gataatgttt tacaactatt tgattatgtt
      841 ttttcagagc tgtttttgga aacggtattt gtagctggca aggtaaattc tttaacatat
      901 tcagatctat caacgtatca gtttttggat aatgagcagc aagtagcaat ttctttgagg
      961 caaggtctga aagagagtga gatggcttct gtccaagtgg cagatagtca agaagccgca
     1021 cttgcagatg tgtcagtttt aactcacaaa tttttgattc cttaccgtgg atttggactc
     1081 ctgagtttga ttggtcctat tgatatggat tatcgtaggt ctgttagttt ggtaaacatt
     1141 atcggaaagg tattggctgc taaattaggt gattattatc gatacctaaa ttctaaccat
     1201 tatgaagttc actgataatg gcagtattca ataagttatt taaaaggagg cactcagtgt
     1261 cagaagaaat taaaaaagac gatttacagg aagaagtgga agccactgaa actgaagaga
     1321 tagttgagga agttattgaa gaaattcctg aaaaatcaga gttagaactc gcaaacgagc
     1381 gtgctgatga gtttgaaaat aaatacttgc gtgctcatgc agaaatgcag aatatacagc
     1441 gtcgctctag tgaagaacgt caacaacttc aacgctatcg ttctcaggat ctggcaaaag
     1501 caatcttacc atcgcttgac aatctagaac gtgccttggc tgttgaaggg ttgacagatg
     1561 atgttaaaaa aggtttagaa atgactcgtg atagccttat tcaagcacta aaggaagaag
     1621 gcgtagaaga agtagaagtc gactcatttg accacaactt tcatatggca gttcagaccc
     1681 tcccagccga tgatgaacat ccagccgata gcattgcaga agtctttcaa aagggatata
     1741 aattgcacga gcgtctgcta agaccagcga tggtagttgt ctataactaa ggtaaatgag
     1801 ttttcgtttt tgtccgtaat gacagtaaac tagatagcaa gttagaagct attcagcttg
     1861 ctgattaaac tatagtgatt gcttagaatt ggaagtaaaa taattcgagt gcttactaag
     1921 ataaattgaa ataaaaagta ataaagtatt ataaaataag aggtattaac atgtctaaaa
     1981 ttattggtat tgacttaggt acaacaaact cagcagtagc agttcttgaa gggactgaat
     2041 caaaaatcat tgctaaccca gaaggcaatc gtacaactcc ttcagtagta tcattcaaaa
     2101 atggtgagat tatcgtgggt gatgctgcaa aacgtcaagc ggtaacaaat ccagatactg
     2161 ttatctctat caaatcaaag atgggaactt ctgaaaaagt ttctgcaaat ggtaaagaat
     2221 atactcctca agaaatttca gcaatgattc ttcaatacct taaaggttat gctgaagact
     2281 atcttggaga aaaagtagaa aaagcagtta ttactgttcc agcttacttc aacgatgcac
     2341 aacgtcaggc aactaaagac gctggtaaaa ttgcaggtct tgaagtagaa cgtatcgtta
     2401 acgaaccaac agcagccgca cttgcttatg gtatggacaa gactgacaag gatgaaaaaa
     2461 tcttagtttt tgaccttggt ggtggtacat ttgacgtatc aatccttgaa ttaggtgatg
     2521 gtgtcttcga cgttcttgca acagcaggtg ataacaaact tggtggtgac gactttgacc
     2581 agaaaattat tgatttcttg gtagaagaat tcaagaaaga aaatggtatt gatctttctc
     2641 aagacaaaat ggctcttcaa cgcttgaaag atgctgctga aaaagctaaa aaagaccttt
     2701 caggtgtaac tcaaactcaa atttcattac cgttcatcac tgctggttct gctggtcctc
     2761 ttcacttgga gatgagctta tcacgtgcta aatttgacga tctcactcgt gaccttgttg
     2821 aacgtacgaa aactccagtt cgtcaagctc tttcagatgc aggcttgtca ttgtcagaaa
     2881 ttgatgaagt tatcctcgtt ggtggatcaa cacgtatccc agcagttgtt gaagctgtaa
     2941 aagctgaaac tggtaaagaa ccaaataaat ctgttaaccc tgatgaagtg gttgccatgg
     3001 gtgctgctat ccaaggtggt gttatcactg gggatgtgaa agacgttgta cttcttgacg
     3061 taacaccatt gtcacttggt attgaaacaa tgggtggtgt cttcactaaa ttgatcgacc
     3121 gcaacacaac tatcccaaca tctaaatcac aagtcttctc aacagcagca gacaaccaac
     3181 cagccgttga tatccatgtt cttcaaggtg aacgcccaat ggcagcagat aacaaaacac
     3241 tcggtcgctt ccaattgact gatatcccag ctgcacctcg tggaatccca caaattgaag
     3301 taacatttga tatcgataaa aatggtattg tatctgttaa agctaaagat ctcggtactc
     3361 aaaaagaaca acacattgtt atccaatcta attcaggatt aactgatgaa gaaattgata
     3421 aaatgatgaa agatgctgaa gcaaatgctg aagcagatgc aaaacgtaaa gaagaagttg
     3481 atcttaaaaa tgaagttgac caagccatct ttgcaacaga aaaaactatt aaagaaactg
     3541 aaggtaaagg ctttgataca gaacgcgatg cagcgcaatc agcacttgat gagttgaaaa
     3601 aagctcaaga atcaggtaac cttgacgaca tgaaagctaa acttgaagct cttaacgaaa
     3661 aagcacaagc tcttgcagtt aaactttacg aacaagcagc tgcagcacaa caagcagctc
     3721 aaggggctga aggtgcacaa tcagctgatt catcaagcaa gggtgatgat gttgtagatg
     3781 gcgaattcac tgagaaataa ttattaatat tgttcagatt catttgaata taagcatgaa
     3841 aactatacta gcatagtaaa gttcttcgtg ataggggttg ctcaataatc tagataagtt
     3901 tcagattaca taagctaatt tcgctatcac taaataaaaa catattaata ataaataggc
     3961 ggggcgcctc gctccgtctg ttttattaag tgtcatatat atgttaacta tttagagctg
     4021 taactgggca agaataattg ttaatctctt caagtgtagt atatgaacaa aatataaagg
     4081 attagataat gaacaataca gaattttatg atcgtcttgg cgtttcaaaa gatgcttctc
     4141 aggacgaaat aaaaaaagct taccgtagga tgtctaagaa ataccatcca gatattaata
     4201 aggaagcggg agcagaggaa aaatataaag aggttcaaga agcttatgaa accttgagtg
     4261 atacacaaaa acgtgctgcc tatgatcaat atggcgctgc tggggcaaat ggcggttttg
     4321 gtggcttcga tggtggtggc tttggtggtt ttgaagacat cttctcaagc ttttttggtg
     4381 gaggagggat gcgcaatcct aatgcaccac gtcaaggaga tgacctacaa tatcgtgtta
     4441 atttaagttt tgaagaagct atctttggag ctgaaaaaga agtatcttat aatcgggagt
     4501 catcatgtca tacttgttca ggaagtggag ctaagccagg tactagccct gtgacttgtc
     4561 aaaagtgtca tggttcgggt gtgattaatg ttgatacaca aacacctcta ggaacaatgc
     4621 gtcgccaagt gacttgtgat gtttgtcagg gtagtggtca agaaatcaaa gagaaatgtc
     4681 caacatgtca tggaactggt catgagaaga agacgcataa agtatcagtt aaaatacctg
     4741 caggagttga aactgggcaa caaattcgtt taacaggaca aggagaagct ggcttcaatg
     4801 gtggtcctta tggtgatctc tttgttatta ttaacgttct cccaagccaa cagtttgagc
     4861 gtaatggatc aacaatttat tatactttga atattggctt tgtccaagct gctttgggtg
     4921 atacaattga tatacctact gtccatggag cggtagaaat gtcaattcct gctggtactc
     4981 agacaggaaa aacgttccgt cttcgcggaa aaggtgcccc taaacttcga gggggtggac
     5041 aaggtgatca gcatgttact gttaacatcg taactcctac taaattgaat gatgctcaaa
     5101 aagaggcatt gcacgctttt gcagaggcaa gtggagataa gatggtacat ccgaagaaga
     5161 aaggtttctt tgataaagtc aaagatgctt tagatgttga ttaaaaaata aaagtctaga
     5221 atcgaaagta ttctagactt ttttgtagtt attgctagag ggattgcttg aacctaaatt
     5281 ttacagtaca atgaatctaa tattgtaggg ggacaatttt tatggtaacc aaagttgaag
     5341 agattcgctc atatttgata gcttctatac aaaatggtaa gttggctcca ggagatcgcc
     5401 taccatctat aagacagtta gctaatcaat tttcctgtaa caaagataca gtgcaacgag
     5461 ttttgatgga attgcgtttt gataattata tctatgcaaa gcctaggtca ggctattacg
     5521 tctttgattc tcatcaagag gaagttgaag aaggggttag tttaccaaac tctgagattg
     5581 caaatatagc ttatgatgat tttagattgt gtttgaatga gacccttatt ggtagggaag
     5641 attacctttt caattattac tatcgtcaag aaggtcttct tgatttaagt aaagcagtgg
     5701 ctaaattaat ggaagaaaca ggggtctatg ttccccttga tgatattgtt attacggctg
     5761 gtactcaaca ggcattattt attttgacac aggttacctt tccaaatcga aaatctcgag
     5821 ttttaataga agaaccgact tatcctcgta tgattgaact aatcaaaaca caaaatttac
     5881 cctatgaaac tattcctcga ggtactcatg gaattgattt tcagcgctta gaggagattt
     5941 tccagacaca atcaattaag tttttttatg ttatacctcg catgcataat cctttgggaa
     6001 catcctataa tccggtagag atgaaaagat taatagagat ggcagagaag tatgatgttt
     6061 atattgtgga agatgactat atgtctgatt ttgcaagtca gtcaccatta cattattatg
     6121 atactcacgg gcgtgttatt tatctaaaat ctttttcaaa ggctattttc cctgctttaa
     6181 gattagctgc gatttgttta ccacaagctt taaaatcaac atttatggct tacaagaagt
     6241 tgatggatta tgatactaat ctgattttac aaaaagcatt agcgctttat attgaaaatg
     6301 gcctttatgc taagaatagt caatatttga aatatcgtta tcagaaagac cttgcaaatt
     6361 caaaatctat tttagctgat caccctaatc tacccccata tagtttacat cacgatagtg
     6421 tattatttga ttgttcgaaa ctcgataact tcaaaatatt acggcaatac ggcgatactt
     6481 tggaaaatta tttttgtcaa aaatcgcatc aatctctctt acaagtaaaa aatgattcct
     6541 gcttaaagca gttcttggga tcgttgtagt ataattgtag ggggacagtt taaaatagat
     6601 attatagttt tcataaatga aaggtggtaa aataaaccca tgacaaggta taaggcacag
     6661 atttcttatg atggttcagc tttttcaggc ttccaaagac aaccaaattg taggacagtt
     6721 caagaagaaa ttgagagaac tcttaagcgc ctaaatagcg ggaacgatgt gataatctat
     6781 ggtgctggtc gcacagatgc tggtgttcat gcttatggac aagttattca ttttgattta
     6841 ccacaggcgc gtgatgtaga aaaattacgc tttggtttag atacacagtg tccagacgat
     6901 attgatattg ttaaagtaga gcaagtgtct gatgattttc attgtcgata tgataaacac
     6961 attaaaactt atgagttttt agtagatatc ggtaggccga aaaatcctat gatgagaaac
     7021 tatgcaacac attaccctta tcctgtcatt attgagctta tgcaagaagc tattaaagat
     7081 ttagtaggca cccatgactt tacaggcttt actgcttcag gtacgagtgt agaaaataaa
     7141 gtacgtacta tttttgatgc gaaaattcag tttgaagcgt ctaaaaatct tttaattttt
     7201 acctttacag gaaatggttt tttgtataaa caggtgcgta atatggttgg taccctgcta
     7261 aaaattggta atggacgcat gcctattagc caaatcaaaa caattttaca ggctaaaaat
     7321 cgggatttag caggtccaac tgcggcagga aatggtttat atttgaagga gattatttat
     7381 gaagacgagg aatgttttag caatttcagg aaatgatatt ttctctggcg gcggcttgca
     7441 tgcagattta gcaacttacg ttgttaataa attacatggt tttgtggcag taacatgctt
     7501 aacagcaatg tcagataaag ggtttgaagt cattcctata gaggcatcta tattaaagca
     7561 gcaactagaa agtttaaaag atgtagagtt tgggtctatt aaactaggat tgcttcccaa
     7621 tgtagaaaca gcacaagttg ttttggaatt tgtaaaatca aaacaagaat gtccagtagt
     7681 cttagaccca gtcttagttt gtaaagaaaa tcatgattta gaagttagcc aattacgtga
     7741 gcagctaatt gcgtttttcc catatgctga tgtgattact cctaatttag tagagactca
     7801 attattgacg ggtctttcaa ttgaaaactt agatcaaatg aaaattgctg ctgagaaact
     7861 ttacgatatg ggggctaaac atgttgttat caaagggggc aaccgtttaa atgctgaaga
     7921 ggctacagac ctttactatg atggtgaacg ttttgaaacc tatgtttttc ctgtagtaga
     7981 tgccaataat acaggtgctg gatgtacatt tgcgtcaagt attgccagcc aattagctat
     8041 gggtaaaaat gtagaagatg cagtcaaaat gtctaaaggt ttcgtctatc aagctattaa
     8101 agcatcggat aagtacggag ttgtccaaca tttttaggag attttaatga ggaaagagaa
     8161 aactagtcaa ttggttcaat tggcaattgt aacagctata tctattgtat tggggatgtt
     8221 tataagtatt ccgacaccaa caggtttttt gacattacta gatgctggaa tattctttgc
     8281 tgccttttac tttggcaaaa aagaaggggc tgtggtaggt gccttagctg gttttttaat
     8341 tgatttattg aaaggttatc ccaattggat gttctttagt ttattgattc atggggcgca
     8401 agggtattta gcgggtttac caggtagaag acgtctttta ggtcttatta gtgcaactct
     8461 tgttatggta ttaggttatg ctatagctag tggattgatg tatggttggg gagctgtttt
     8521 accagatatt cctggcaata ttatgcaaaa tatggttggt atggtcgttg gttttgctct
     8581 taataagtct ctagaaagag tgaagaaatg atactagatt ataataagtt aaagcaggaa
     8641 actaaagcta ttgtagttga tattattgag cgtagtgctc ttaaaaaagg tcaaattttt
     8701 gtactaggtc tatcatctag tgaggtttct ggtggtttaa ttggaaaaaa ttctagttct
     8761 gaaattggtg aaattatcgt tgaagttatc ctcaaagagt tacattcacg cggcatctat
     8821 ttagcagttc aaggttgtga acatgttaat agggcgttgg ttgttgaagc agaattggct
     8881 gaacgtcaac agttggaagt cgttaatgtt gttcctaatc tacatgctgg cggtagtggt
     8941 caggttgcag cttttaagtt gatgacatca ccagttgagg ttgaagaaat tgtggcacat
     9001 gctggaattg atattggaga tacttctatt ggaatgcata tcaagagagt acaagttccc
     9061 ttgattccaa ttagtcgtga gttgggaggg gcacacgtta cagcactagc tagtcgtcct
     9121 aaattaattg gcggtgctag agcaggatat acgagcgatc caatccgtaa attttaaaat
     9181 aaacctctgc tatgcagagg tttattgttt atctcaacga gagtggttat tggagaaatt
     9241 gtatgttggt aagtcaatac cttcttcaag aatagctttt tgataaagtt tgtaaaattc
     9301 agcatagatg tcaaactgag caccgttttg cgtgaagata ttaatacgat aaacaaattg
     9361 accattagca ttggttgtag gaccaaaaac agtaggctct ccaacgatag caggatgttt
     9421 tgaaacatac tcttcattga tttttgttac aatatcagaa atctgatcaa ggtttgtatg
     9481 aacaaataaa ggaatatcaa tttgagcacg catattgcta cgagacttat tgctgacaat
     9541 tgtaatatta cgattaggaa tgaagtgtaa ggttccatcg aaatcatgaa tttgtgtggt
     9601 gcgtatccca acattagtaa cagtgcctga tacagcacca acattaataa tatcaccaac
     9661 atcaaattgg ttttctagca gaataaaaaa gccattgaca acatctgata agaagccttg
     9721 agcaccaagt ccaatagcaa ccccagcaat accagcccca gctaataaac tagaaattgg
     9781 aacgcccaat atacttaaaa tccaatagaa tagaaagaaa taaagggtat agtttaaaac
     9841 attgtgagaa agtttagcta atgttttttg acgagctact ttttgtctag aaaaggcgag
     9901 tgagcgatta acagtctttt cgaaaagata attgatgact tgcctaacga taacgaatgc
     9961 aatgattaat aataagattg atattagttt ggtgaagaaa gtaaataaaa cttcctctac
    10021 attaagatgg tcaataaatt tttctaaagt aatcatagta atagtctagt aaaaaaaaag
    10081 ccatagagca agtattttaa aagcttatcg ttaagcagtt taaagcttct tttttgcata
    10141 aaaactttgt aaaacgtttt aaattatggt aaactaaact gtatctaaat aattttaagg
    10201 agaaatgact taatgtctac atcatttgaa aataaagcta caaaccgtgg tattatcaca
    10261 tttacaatta gtcaagatga gatcaagcca gctcttgatc aagcattcaa caaagttaaa
    10321 aaagatttga acgttccagg attccgtaaa ggacatatgc ctcgtacagt tttcaaccaa
    10381 aaatttggtg aagaagcact ctacgaaaat gcattgaatt tagtgttgcc aaaggcttac
    10441 gaagcagctg ttgcagaact tggtttagac gttgttgctc aaccaaaaat tgatgttgta
    10501 tcaatggaaa aaggtcaaga ttggaagttg acagctgaag ttgttacaaa accagaagtt
    10561 aaacttgggg attacaaaga cctttcagta gaagttgatg cttcaaaaga agtttcagat
    10621 gaagaagttg atgctaaagt tgaacgtgag cgtaacaacc ttgcagaatt gactgttaaa
    10681 gatggtgaag cagcacaagg tgatacagtt gttattgact ttgtaggttc agttgatggt
    10741 gttgaatttg atggtggtaa aggagataac ttctcacttg aacttggttc aggtcaattt
    10801 atcccaggat ttgaagaaca attagtaggt tctaaagctg gtcaaactgt agacgttaat
    10861 gtaacattcc cagaagatta tcaagcagaa gatcttgctg gtaaagatgc taaatttgtt
    10921 acaacaattc atgaagtaaa aactaaagaa gttccagcac ttgatgatga acttgcaaaa
    10981 gatattgatg atgaagttga aacacttgat gagttgaaag cgaaatatcg caaagagcta
    11041 gaatcagcaa aagaaattgc atttgatgat gcagttgaag gtgtagcaat cgaattagct
    11101 gttgccaatg cagaaattgt tgaattacca gaagaaatgg tccatgatga agtacatcgt
    11161 gctatgaatg agttcatggg taatatgcaa cgtcaaggaa tttcaccaga aatgtacttc
    11221 caattaacag gtacaactga agaagacctt cataagcaat accaagcaga tgctgacaaa
    11281 cgcgttaaaa ctaaccttgt tattgaagca attgcagctg cagaaggctt tgaagcaaca
    11341 gatgaagaaa tcgaaaaaga aatcacagat cttgcttcag aatataatat ggaagctgac
    11401 caagtacgcg gtctactttc agcagatatg cttaaacatg atattgcaat gaaaaaagcg
    11461 gttgacgtta tcactagctc agctactgta aaataattgc ttattaaatt ttgaaaagat
    11521 tgggcttccc agtcttttta tttgtgatta ttcttttgac gaatagctaa aaatagcgta
    11581 aaatagtaaa tagcgaaaat ttgaaaatat gacttatcta ttttctatct ataaagaata
    11641 ttgctaaaca aaatgatagt cttacacaat taaggagaat aactttggaa ttagaagtat
    11701 ttgctgggca agaaaaaagt gaactttcga tgattgaagt tgctcgcgct attttagagc
    11761 aacgtggtcg tgataacgaa atgtacttca gcgatttggt aaatgatatt caaacctacc
    11821 ttggaaaatc tgactcagca atccgtgagt cattgccatt tttctattca gatttgaata
    11881 cagacggaag ttttattcca cttggcgaaa acaaatgggg acttcgttca tggtatgcta
    11941 tcgatgaaat tgacgaggaa atcatcactc ttgaagaaga tgaagatggc gctccaaaac
    12001 gtaagaagaa acgtgtcaat gctttcatgg atggtgatga agatgctatt gattacaatg
    12061 atgatgatcc agaagatgaa gatttcacag aagaaacacc aagtttagaa tatgatgaag
    12121 aaaaccctga cgatgaaaaa tcagaggtag aatcgtatga ttctgagatt aatgaaatca
    12181 ttcctgatga agatttagat gaggatgtag aaatcaacga agaagaggaa gaagaggaag
    12241 aagaggttta gatggggatt ttgtatttga caaatagtca gatatagttt aaactattat
    12301 tcgggcacct cttatgaggt cagataaagc tccctagtga tagggggcta ttttgttttt
    12361 gggaaagata cagaatggtt ccagatagtt ttctgagtag gcccttgatc agatagtttc
    12421 tactagtttt gactagagaa gctggtatta tttatcaatc atgtgttttg ggttttaccc
    12481 gtaaactaac tgttagatta acatttaatt taaggagttt ttaatgacaa agtatatttt
    12541 tgttacaggt ggtgtggtct cttctattgg taaggggatt gttgcagcaa gtcttgggcg
    12601 cttactaaaa aaccgtgggc taaaggtgac aattcaaaaa tttgatccat atattaatat
    12661 tgacccaggg acaatgagcc cttatcaaca tggggaagtt tatgttacag atgatggtgc
    12721 agaaactgac cttgaccttg gacactacga gcgttttatt gatattaact taaacaagta
    12781 ttcaaatgtt acaacgggta agatttatag tgaagttctt aaaaaagaac gccgtggaga
    12841 ataccttggt gcaactgttc aagttatccc acacgttacg gatgctttga aagaaaaaat
    12901 caaacgtgct gcgacaacta cagattcaga tgttattatt actgaagttg gcggtactgt
    12961 tggagatatt gagagccttc cgtttttaga agcacttcgt caaatgaagg cagatgttgg
    13021 ttctgataat gttatgtaca ttcatacaac gttattacca tacctaaagg ctgccggcga
    13081 aatgaaaaca aaaccgactc aacattctgt taaagagtta cgtggcctag gtatccaacc
    13141 taatatgctt gttatccgta cagagcaacc agcaggtcaa agtattaaga ataaattggc
    13201 acaattttgt gatgttgcac cagaggctgt tatagagtcc cttgatgttg accatattta
    13261 tcaaattcca cttaacatgc aagctcaaaa tatggatcaa attgtttgtg atcacttgaa
    13321 gcttgagaca cctgctgctg atatgacaga atggtcagca atggttgata aagtaatgaa
    13381 ccttgagaaa aaagttaaaa ttgcattggt tggtaaatat gttgaattac ctgatgctta
    13441 cctttcagtt gtggaggccc ttaagcactc agggtacgtc aacgatgttg ctattgacct
    13501 aaaatgggta aatgctgctg aagtgacaga agataatatt aaagaacttg tcggagatgc
    13561 agatggtatc atcgttcctg gtggatttgg ccaaagaggt agtgagggta aaattgaagc
    13621 aattcgctat gcacgtgaaa atgatgttcc aatgcttggt gtttgtctcg gaatgcagct
    13681 aacttgtgtc gaatttgcgc gtaatgtttt aaaccttcat ggagctaatt cagcagagct
    13741 tgaccctaaa acaccgttcc caatcattga tatcatgcgt gatcaaatcg atattgaaga
    13801 tatgggtggt acacttcgcc ttggtctata tccatgtaaa ttgaaagcag gttcaagagc
    13861 tgcggcagct tataataatc aagaagttgt ccaacgtcgt caccgtcacc gttatgaatt
    13921 taatacaaaa ttccgtgagc aatttgaggc tgctggattt gtcttctcag gggtatcacc
    13981 ggataatcgt ttgatggaag ttgttgagtt accagagaaa aaattctttg ttgcagcaca
    14041 ataccatcca gaactacaat cacgtcctaa ccatgctgaa gagctttaca cagcttttgt
    14101 aacagcagca gttgaaaata tgaaataact ttatcgcctc ccagtattag gaggcgattt
    14161 tattttttta gatagtttca ctatcaaagt atcattagct cttttctttt tctaaaaata
    14221 aggtataata ggttgcatga aaaagattcg attatcaaag tttattaaaa tgattgttgt
    14281 tattttgttt ttaattagtg tagcagctag tttttatttt ttccacgttg cccaagttcg
    14341 agatgataaa tcctttattt caaatggtca acgtaagcct ggaaactctt tatatgctta
    14401 tgataaatcc tttgataagc tattaaagca aaaaatagaa atgacaaacc aaaatataaa
    14461 gcaagttgct tggtatgttc ctgctgctaa gaaaactcat aagacagttg ttgtcgttca
    14521 tggttttgcg aatagcaaag agaatatgaa ggcatatggt tggctgtttc ataagttagg
    14581 atacaatgtt cttatgcctg acaacattgc acatggtgaa agtcatgggc agttgatagg
    14641 ctatggctgg aacgaccgcg agaacattat caaatggaca gaaatgatag tggataagaa
    14701 tccatcaagc caaattactt tatttggtgt ttcaatgggt ggagcaacag tcatgatggc
    14761 tagtggtgaa aaattaccta gtcaggttgt taatatcatt gaagattgtg gttattctag
    14821 tgtttgggat gaattaaaat ttcaggctaa agagatgtat ggtttaccag ccttcccact
    14881 cttatatgaa gtttcaacaa tttctaaaat cagagcaggt ttttcgtatg gacaagcaag
    14941 tagtgtcgaa caattgaaaa agaataattt accagccctc tttattcatg gtgataagga
    15001 taattttgtt ccaacaagta tggtttatga caactataaa gctacagcag gtaagaaaga
    15061 gctttatatt gtaaaagggg caaaacatgc gaaatctttt gaaacagagc cagaaaaata
    15121 tgagaaacgt atctctagtt ttttgaaaaa atatgaaaaa taatattgac aacaatatga
    15181 taagttgata taatttatta tgttgttaag cggggatggc ggaattggca gacgcgcaag
    15241 actaaggatc ttgtgaccgc ttttggtcgt gagggttcaa gtccctctct ccgcagacta
    15301 gtcgagttca tttggaactc gactttttgc atatttaaga taaagatgga gtatcacatg
    15361 tcaaaagtac gtggctttga attagttagc caatttagca ataaagagtt attaccaaaa
    15421 cgagaaacag ctcacgcagc tggctatgat ttaaaagtag ctaaaaaaac ggttattgaa
    15481 cctggtgaaa ttacattggt cccgaccggt attaaagcac acatgcaacc aggggaggtc
    15541 ctgtaccttt atgatcgttc atcgaatcca cgtaaaaaag ggattgtcct aataaattca
    15601 gttggtgtta ttgatggtga ctactataat aaccaagtca atgaaggaca tatttttgca
    15661 cagatgcaaa atattactga tcaggctgtt attttagaag aaggggaacg gattgttcag
    15721 gctgtttttg ccccattctt attggcagat gacgatcagg ctacaggaat gcgtacaggt
    15781 ggttttggta gtactggaaa ataaaattat tttacgaata atgttagaaa aggctagact
    15841 tacttcttag ccttttttga tgagagtaaa cttatatttt ctctaacttg gtgtgatata
    15901 atgagtgtac tagttatttg gtaccaagct atcatattaa tttagaagtt ataaaggagg
    15961 cctgagtggc taagaaaaaa tcagtcttca cctgtcaaga gtgtggttac caatcaccta
    16021 agtatttggg acgttgtcca aattgctcag cctggtcttc atttgtagaa gaggttgaag
    16081 ttcaagaagt taaaaatgca cgagttagtt tgaacggtga aaagtcccga ccgacgaagt
    16141 taaaagatgt ttcctcaatt aactattcta gaactaagac tgatatggat gaatttaacc
    16201 gtgttctagg aggcggtgtt gtaccaggaa gtttggttct gattggagga gaccctggga
    16261 taggtaaatc tactttatta ctacaagtat caactcagct agctaataaa ggtacagtat
    16321 tatatgtctc aggtgaggaa tctgcagaac agattaagtt acgaagtgag cgtctaggcg
    16381 atatcgataa tgaattttac ctttatgccg agactaatat gcaaagtatt cggtcggaga
    16441 ttgagaagat taaaccagat tttcttatta ttgattctat acaaactatt atgagtcccg
    16501 aagtatcaag tgtccaaggc tctgttagcc aagttcgcga agtgaccgct gagttgatgc
    16561 agttagctaa aactaataat attgctactt ttattgtagg tcatgtgact aaggagggaa
    16621 cattagcagg ccctcgaatg ctagaacata tggtggatac ggttctttat tttgaagggg
    16681 agcgtcatca cacgttccgt attttacggg cagtcaaaaa ccgttttggt tcgaccaatg
    16741 aaataggtat ctttgaaatg cagtcgggtg gtctagttga agttttgaat ccaagtcaag
    16801 tttttcttga agaacgttta gacggtgcaa caggatctgc tattgttgtt actatggaag
    16861 gaacaaggcc tattttagca gaggttcaag ctttagtgac tccaactgtt tttggaaatg
    16921 ctaagcgcac aaccacaggt cttgacttta atcgtgtcag tcttattatg gcagttctag
    16981 aaaaacgttg tggtttattg cttcaaaatc aagatgccta tttaaaatca gcgggtggtg
    17041 ttaaattgga tgaaccagca attgatttag ctgttgctgt agctattgct tccagttaca
    17101 aagaaaaacc cacaaatcca caagaatcat ttattggaga aattggactc actggtgaga
    17161 ttcgtcgcgt gacacgtatt gagcaacgta ttaatgaggc tagtaaattg ggctttacta
    17221 aaatttacgc accaaagaat tctctagctg ggattgaaat acctaaaggc attgatgtta
    17281 ttggtgtgac aactgttagt caggtcttaa aagcagtgtt ttcaacataa tgattagatt
    17341 cggttcagcc gaatcttttt tggtggcgag agacatttcg tgaaaaataa tgggcaaaat
    17401 gataatataa aagggttggt atttagtaaa tatcgaatga tgttacacta acaaaggaga
    17461 tctctatgac cacttatttt gataactttt taaaaaccaa tcaagcctat gctgacttac
    17521 atggaacagc tcatctacct atcaagccaa agacaaaggt tgctattgta acctgtatgg
    17581 attctcgttt acacgttgct caagctctag ggctagcttt aggagatgct catattttac
    17641 gtaatgctgg tggacgagtt acagccgatg ttctacgttc tttagttatt tcgcaacaac
    17701 aattaggaac acgagaaatc gttgttctac accatacaga ttgtggagca cagacgttta
    17761 ctaacgaagc ttttgctgct caattacaga gagatttggg tgttgatatg catggacatg
    17821 attttcttcc ttttaatgat attgaagaaa gcgttcgtga agatgtggca aaattacatg
    17881 cttctccatt aatcccaaat gatgttgtta tttctggtgc tatttatgat gtagatactg
    17941 gtcgtatggt ggaagttaaa tagaaaaaat cctgtcctta gcgatgggtt tttttatttt
    18001 agaaaactag atttaaaggt aacagttagg cttatcatgc cttatgctgg aattcagaca
    18061 gaggaaacat cttttgctat actgaaacta tattataaaa gcggaggaaa tttaaaatga
    18121 aaaaatatga tgtcattgtt cttggttttg gtaaagcagg taaaacatta gcagcaaaat
    18181 tagcaacaca aggtaaaagc gttgcaatgg tcgaagaaga cgataaaatg tatgggggaa
    18241 catgtatcaa cattggatgt attccgacaa aaacgctttt agtatcagct agcaaaaatc
    18301 acgattttca agaagctatg actactagaa atgaagttac aagtcgttta cgtgctaaaa
    18361 attttgctat gttggataat aaagacactg tggatgttta taatgctaaa gctcgcttta
    18421 tatcaaataa agtggtcgaa ttaacaggag gagctgacaa acaagaactt acagctgatg
    18481 ttattattat caatactggt gctaagtcag tacaattacc aattccagga ttagctgatt
    18541 ctcaacatgt ttatgattca acggcaatcc aagaattggc tcatcttcct aaacggttag
    18601 gtatcattgg aggcggtaat attgggcttg aatttgcgac tttatattcc gaattaggga
    18661 gcaaagtgac agttattgat agtcaatctc gtatctttgc gcgtgaggaa gaggaattat
    18721 cagagatggc tcaagattat ttagaagaga tggggatatc ttttaaatta tcagccgata
    18781 tcaaatctgt gcaaaatgaa gatgaaaacg ttgttattag ctttgaggat gaaaaattaa
    18841 gctttgatgc agtattatat gcaacaggac gtaagccaaa tacagaaggt ttagcacttg
    18901 aaaatacaga tattaaattg acagaacgtg gagctattgc tgttgatgaa tattgccaaa
    18961 cttctgttga gaatattttt gcagttggtg atgttaatgg cggccctcaa ttcacatata
    19021 tttctttaga tgactcacgt attgtcttaa attatttgaa tggtgataag gattattctc
    19081 taaaaaatcg aggagcagta ccgacttcaa cctttacaaa tcctccttta gcaacggtag
    19141 gtctagatga aaagaccgct aaggaaaaag gatatcaagt taaatctaat agccttcttg
    19201 tttctgccat gccacgtgca catgttaata atgaccttag aggtattttt aaagttgtgg
    19261 tagatactga gacaaatctt attttgggag ctcgcttatt tggtgcagaa tcccatgaat
    19321 taattaatat catcacaatg gcaatggata ataaaatccc atatacttat ttccaaaaac
    19381 aaattttcac tcacccaaca atggtagaga attttaatga tttgtttaac ttttaagaat
    19441 atttttaaaa agtaaggcaa ttttgcttta ctttttttat c
//
LOCUS       DATROY010000018        19473 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.018, whole genome shotgun sequence.
ACCESSION   DATROY010000018 DATROY010000000
VERSION     DATROY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..747
                     /locus_tag="VBD11_000604"
     CDS             <1..747
                     /locus_tag="VBD11_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953575.1"
                     /translation="IVEDFNPYSNLDNLEIKKIRLNGSQKQKVEQEKTKSPTPQKEAV
                     KEQTEQKISGKTQNVEKKSETVATPQQSSTAQTSVQQPAPVQPVVQESKASQEEINAA
                     HDAISAYKSTVNIANTAGVTTAEMTSLINTQTSNLSDVEKALGNNKVNNGAVNVLRED
                     TSRLENMIWNRAYQAIEEFNVARNTYNNQMKSETVPVDNDIEAILAGSQAKISHLDNR
                     IGARHMDQAFVASLLEVTEMSKSISSRIKE"
     gene            814..1359
                     /locus_tag="VBD11_000605"
     CDS             814..1359
                     /locus_tag="VBD11_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953576.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1510..1599
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1755..2321
                     /gene="thiW"
                     /locus_tag="VBD11_000606"
     CDS             1755..2321
                     /gene="thiW"
                     /locus_tag="VBD11_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6953577.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2318..2872
                     /locus_tag="VBD11_000607"
     CDS             2318..2872
                     /locus_tag="VBD11_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6953578.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            2876..4162
                     /locus_tag="VBD11_000608"
     CDS             2876..4162
                     /locus_tag="VBD11_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6953579.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4150..4818
                     /locus_tag="VBD11_000609"
     CDS             4150..4818
                     /locus_tag="VBD11_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6953580.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            4902..5582
                     /gene="tenA"
                     /locus_tag="VBD11_000610"
     CDS             4902..5582
                     /gene="tenA"
                     /locus_tag="VBD11_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6953581.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            5584..6381
                     /gene="thiD"
                     /locus_tag="VBD11_000611"
     CDS             5584..6381
                     /gene="thiD"
                     /locus_tag="VBD11_000611"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6953582.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6383..7153
                     /gene="thiM"
                     /locus_tag="VBD11_000612"
     CDS             6383..7153
                     /gene="thiM"
                     /locus_tag="VBD11_000612"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6953583.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7167..7838
                     /locus_tag="VBD11_000613"
     CDS             7167..7838
                     /locus_tag="VBD11_000613"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6953584.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            7965..9224
                     /locus_tag="VBD11_000614"
     CDS             7965..9224
                     /locus_tag="VBD11_000614"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6953585.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9326..9880
                     /locus_tag="VBD11_000615"
     CDS             9326..9880
                     /locus_tag="VBD11_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6953586.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            9873..11156
                     /locus_tag="VBD11_000616"
     CDS             9873..11156
                     /locus_tag="VBD11_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6953587.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11179..12039
                     /locus_tag="VBD11_000617"
     CDS             11179..12039
                     /locus_tag="VBD11_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6953588.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12041..12961
                     /locus_tag="VBD11_000618"
     CDS             12041..12961
                     /locus_tag="VBD11_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6953589.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(12978..13433)
                     /locus_tag="VBD11_000619"
     CDS             complement(12978..13433)
                     /locus_tag="VBD11_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6953590.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13504..13617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            13615..14124
                     /locus_tag="VBD11_000620"
     CDS             13615..14124
                     /locus_tag="VBD11_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6953591.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELTSLLVGAVLVDK
                     LSRHVDFTK"
     gene            14264..16222
                     /gene="ligA"
                     /locus_tag="VBD11_000621"
     CDS             14264..16222
                     /gene="ligA"
                     /locus_tag="VBD11_000621"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6953592.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16234..17253
                     /locus_tag="VBD11_000622"
     CDS             16234..17253
                     /locus_tag="VBD11_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6953593.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            17257..>19473
                     /gene="pulA"
                     /locus_tag="VBD11_000623"
     CDS             17257..>19473
                     /gene="pulA"
                     /locus_tag="VBD11_000623"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6953594.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDI"
BASE COUNT         6325 a         2946 c         3969 g         6233 t
ORIGIN      
        1 attgtggaag attttaatcc ttattctaat ttagacaatt tagaaataaa aaaaataagg
       61 ttgaatggct cccaaaaaca aaaagtagaa caggaaaaaa ctaaatcgcc aactcctcaa
      121 aaagaggctg taaaagaaca aactgagcaa aaaatatctg ggaagactca aaacgtagaa
      181 aagaaatctg aaactgtggc aactccacaa caaagttcaa ctgcgcaaac ttctgtccaa
      241 cagccagctc cggttcaacc agttgttcaa gaatctaaag cttctcaaga ggagattaat
      301 gcagcacatg atgccatttc agcgtataaa tcaacagtca atattgctaa tacagccggt
      361 gtaacaactg cggaaatgac ctcgctcatt aatactcaaa cttctaatct ttctgatgtt
      421 gagaaagctt tagggaataa taaggttaat aatggtgcag tcaatgtatt gagagaagat
      481 acatctcgtc ttgagaatat gatttggaat cgtgcttacc aagctattga agaattcaac
      541 gtcgctcgta atacttataa taaccaaatg aagtcagaaa cagttccagt tgataatgat
      601 attgaagcta ttttagcagg ttctcaagct aaaattagcc atttggacaa tcgtatcgga
      661 gcgcgccaca tggatcaagc ttttgtagct agtttattag aagttactga gatgagtaaa
      721 tcaatctcat cgcgtataaa agaatagaca ctgctatcaa ggcgatctta aacttttgta
      781 ttgagctaac ctaaaagata gaaagagact aatatgaaaa aaataacaac tttaatctta
      841 gctagtagct tattactagt tgcaacgaca tcggttaaag ctgatgataa ctttgaagta
      901 ccaacgcgtt atgttaaaat gagtgaaaaa tcaaaagcat tttatcaaag attacaagaa
      961 aaacaacgta aaacacatac tactgtgaag acttttaata attcagaaat aaggcatcaa
     1021 ctacctctta aacaagaaaa ggctaggaat gatatctaca atttaggcat tcttatttct
     1081 caggagtcta aagggttcat ccaacgtatt gataatgcct attctttgga aaatgtctca
     1141 gatattgtta atgaagctca ggctttgtat aaacgcaact atgatttatt tgaaaaaatc
     1201 aaatctacac gtgataaggt tcaagtctta cttgcatcgc atcaaggtaa tacagactta
     1261 aaaaactttt atgctgagtt ggacgatatg tatgaacatg tttatctcaa cgaaagtaga
     1321 gtggaggcga taaacagaaa tatccaaaaa tataattagt ttctaaacta acaaacattc
     1381 ctaaatataa gacattaaac cctacttatt gattagtgag tagggtttta ctgttttaaa
     1441 tagctttctg ctcagaatgt aagccttgtc atttcaaagg aactatgtta ttattcttaa
     1501 gtaaattaaa taggacattt ggggtgcgta acagctgaga ttatacccat tgaacttgat
     1561 acagttagta ctgtcgaagg gaaatgtgtg ccattacaag ttttttgtaa tggaaaaggg
     1621 cgtagtgtct tcttaggaga cagctagctc tagttaatca gataataaac tccgtttgct
     1681 tgcagatgga gttttttgtc gtcaaaattt tttataggtt gttgcccctt atctgtcata
     1741 gagaaggagt tatgatgact aatcataaaa caaaaaaatt aacggtttta gcaattatga
     1801 ttgctttaga tgtttttcta acgcccattt ttagaataga aggcatggcc tttatgtcta
     1861 gtgtggttaa tattatcgct ggagttataa tgggaccaat ctatgcatta gttatggcac
     1921 ttgtaacggc ttttattcgt attttaactc aagcaggagt agcatctgtt gcacctttag
     1981 ctattctagg agctggtcca ggagctttct tagcgggttg gttttatcgg ttttatgcga
     2041 aagactggat gtcttgggtg ggtgagttta taggaacagg gataattgga gcacttttat
     2101 cagctccaat tatggtctgg ttttggactt ggacagcgga tggaaatcac gaagtgttag
     2161 ctaaagcatc tgcagctcaa tccatttttc tttttatgcc aaggtttata ggggcgacta
     2221 taattggcgg tgttattgct ttgttgctac ttcagggatt aaagcgggtt cctgtattta
     2281 tgacaataca agcattattt aaaggagaaa aagtaaaatg aaattgggtt taaaagatgt
     2341 attattgatt gctttattag cggttgttct gggcgttgtt tactttggag caggctatat
     2401 ttcaaatgct tttgtacctt ttgtaggtcc tatagcgcat gaagttattt atggtatttg
     2461 gtttgtggca ggtccaatgg ctctttatat attgaggaaa ccaggaaccg ctattgtagc
     2521 agaattgtta gctgctttga tagaggtttt aattggatca atttatggac caagtgtact
     2581 cgttattggt acccttcaag gtttagggtc agaattaggc tttacgctgt ttaggtatca
     2641 taattacaag ttgccagctt ttatattgtc tgctattttg acatcaattt ttagttttgc
     2701 atggagtttt tatgccaatg gacttagtgc attttcattt tcatataaca ttctgatgct
     2761 tatagtaaga actgtttcat ctattatttt cttcttatta acaaagaata tatgtgacca
     2821 attgcataga agtggtgtac tgaatgctta tgggattcat gaagggaagt agcctatgct
     2881 atctgttgaa aaattagctt acacccatgg cgatagtcat tatttatttg atgaagtgac
     2941 ttttagtctt aacccaggag aacgaatttt aattagtggc tattcagggt gtggtaagag
     3001 tacattggct cttttactat cagggttaaa agaaagtggg aaaggtcaag ttttattaaa
     3061 cggctcatta atagaaccaa gcgatgtggg ttttcttttt caaaatcctg atttgcaatt
     3121 ttgtatggat actgtggcgc atgagttgta ttttatttta gaaaatttac aaatagaacc
     3181 ggagcaaatg caagatagaa gtgagtttgt attggcacaa gttggtttaa aaggatttca
     3241 gaatcgtttg atttatactt tatctcaagg tgaaaaacag cgattagcat tggcgactat
     3301 ttttttaaaa tctcctaaac ttattatttt agatgaagct tttgcgaatt tggatcaaga
     3361 gagtgccagt caactattac aattagttct taattatcaa gcaaacaatc aatcaatgct
     3421 gattgtaatc gatcatttga ttacttatta tcaagatata atggatcact atttttggtt
     3481 ggaaaaatgg ttgataaggg tcgattttga ttatatgctc aatcgtttga atgtcttcga
     3541 attagaaaag aaaagtcata atacaggtga caaactgtta tcaattaaag atttccaagt
     3601 taaactttca aaaaacaaat tcatctctta ccttgatttt gatttagcaa gtggggagag
     3661 gttgtgctta gatggaccaa gtggcgtggg gaagtctagt ttctttatgg gattgttagg
     3721 gctctatagg actaaaggaa agaaacaatt tactcataga aagcaaattc ctataagctt
     3781 tttatttcaa aatcccttag atcaatttat tttttcaaca gtttatgacg aaatttttca
     3841 ggtttgcaag gatagtaaca aagcgaggga tattttggaa accataaatc tttgggataa
     3901 aaaacagttt tcaccatttc aactaagtca aggccaacag aggcgattag ccattggcag
     3961 tattttagct agtgatagta aacttctatt attagatgaa ccaacttatg gacaagatgc
     4021 ctatcacgct aatatgatta cgactcttct tttgagttat tgtcacaaaa atcattgtgg
     4081 ggttattttt actagccatg atcctcactt aaaagagtgt tttgcaacac gtttcttgga
     4141 ggtgtcatct tgttcaattt gaaaaactat catccagcta tcaccttcct atgtttatta
     4201 ataccaacct tttcgtttag tttcacattg aggctaagaa cgtctttgtt atttctaatt
     4261 atagtagtaa cactgcaatg cttcgtaaag gtttctttaa aaacttgggc gaaggtcaat
     4321 ttgatctcat ttgtgatggg actctcttta tttttaggga cgtatttttg ggggaaatta
     4381 ccccatcaat tcgtcttagc tagtctagtg gcctgtcgtc cgcttatttt tatgaacgtg
     4441 ggtttgttat ttcatgcatc acattctaat tatgatttta tagaatctct ttatcaaact
     4501 ttcaaagtgc ctagtcattt tgcatatggt atttttgcgg tctttaatct tttaccatta
     4561 attaaattac aataccaacg taatcgactc gcattccgac tgaagaatca ggtaacttgg
     4621 gcattatcgc ctagactaat attgtcagtt ttattaaaga cgatatattg ggtagaacag
     4681 ttagagttag caatgctttc taaaggtttt gaagtgggta aagagcgaac acatgcttca
     4741 acttatcccg ttcgttttcg agattatagt ttacttggta tgagtatttt attggctata
     4801 ggaatgattt ttaaataagt acaattagtt tagtaataca attgtgcatt tatatagagt
     4861 gagcatgttc tgctcatgag atcataacaa aggagattac tatgttaaaa gatgagatga
     4921 ttgctaaagg aacaaaatat atacaatcca tctatcaaga tccatttatt caaggaattg
     4981 taaaagggcg tttagaccat gacgtaatct gccattattt acaggcagat aatatctacc
     5041 ttgggaaatt tgctgatatt tatgccctgt gcttggcaaa atctgataat ttacgagata
     5101 aacaattttt ccttgaacaa gttgacttta cattaaatcg ggagttagca gatggtgaag
     5161 gtccacatca agccttggca gcttatacta atcgatctta tcaggatatt atcgaaaagg
     5221 ctgtatggta cccgtcagca gatcattaca ttaaacatat gtattttcat ttttacgaaa
     5281 atggtattgc tggtgcttta gctgcaatga gcccatgtcc atggatttac catcaattgg
     5341 ctaaaaaaat aattgaggag aaccagtttt tgaatggtaa cccttttaat aattggataa
     5401 ctttttatgc taacgataca gttgaagagt taatggaaaa ctattttcgt atgatggatt
     5461 attatgcgca gaatttgtca aaagagaagc aagctgattt ggtagatgct tttgtgaaat
     5521 cttgtcagca tgagagacgt ttttttcaaa tggcaataaa tcaagaaaaa tgggaggatt
     5581 aatatgactt atttagcacc agttttaact attgcaggaa ctgatcctag cggtggagca
     5641 gggattatgg cagatttaaa gacatttcaa gctcgtcgga cttacgggat ggctgttgtg
     5701 actagtgttg ttgctcagaa cacttgtggg gttagaggcg ttcagcatat agaaacagct
     5761 atcattgatc aacaactagc ttgtgtttat gatgatatta agccaaaggc ggtcaaaaca
     5821 ggaatgttag cggagaggga aacgattagt ttagtcgctt catatttaaa aaaatatccg
     5881 caaccttatg tattagatcc tgttatggtg gcaactagcg gacatcgttt aattgattca
     5941 gatgccgtag aagcattaaa agaagactta ttgccattag caactattat cacaccaaat
     6001 ttaccagaag ctgaggtttt agtgggatat gacttatcag atgaagtaag tattattaaa
     6061 gcaggttacg atattcaaaa acaatattct gttagaaatg ttcttataaa gggaggacat
     6121 ctagatggtt tggctaaaga ttatttattt ttggaaaaag aagggttgat aactcttagt
     6181 aatcaacgta tcaatactat tcatactcat gggactgggt gtacatttgc agcagtcgtt
     6241 gcagcggagt tagctaaagg gcaatcaatt ctaaacgcgg taagtaccgc taaatcattt
     6301 attacttcag ctatcgaaac agcgcctgag ttgggtcttg gaaacggtcc tgtcaatcat
     6361 actagctatc aaggagacta aaatgtattt agaacaacta aaagaggtaa atcctttaac
     6421 catttgtatc actaataatg tggtcaaaaa ctttacagcc aatggtttat tagctttagg
     6481 agcttctccg gctatgagcg agtgcataga ggacttagaa gacttgttaa aagttgcaga
     6541 tgcattatta attaatatag gaacactaac aaaagaaagt tggcaacttt accaagaagc
     6601 aatcaaaata gcaaataaaa accaagttcc agttgtatta gatcctgtag cggcaggagc
     6661 aagtcgtttt cgattagaag tgtcacttga tttattgaag aattatccta ttagtttact
     6721 tagaggaaat ggtagtgaaa tcgctgcttt ggttggtgaa aaacaagcgt ctaaaggggc
     6781 agatggggga aaagtggctg atttagaatc aattgctgta aaagccaatc aagtttttga
     6841 tgtgccagtg gttgtcacag gagaaactga tgctatcgct gttagaggag aagttcgctt
     6901 attacaaaat ggtagtcctt taatgccact tgttacagga actggatgtc tattaggggc
     6961 agtattggct gcttttattg gtagttctga cagaagcgat gatcttgctt gtctgacaga
     7021 ggccatgact gtttataatg ttgctggaga aattgcagag aaagtggcaa aaggtaaggg
     7081 agtgggaagt ttccaagttg catttttaga tgccctttct cagatgaaat cagaaatgat
     7141 tatggacaaa taggcggtgt cagtagatga aagatacatt gaaattatat tttgtttgtg
     7201 ggactgtgga ttgctctagg aagaatatat tgactgttgt tgaagaagcc ttacaagcag
     7261 gtataactct atttcaattt agagaaaaag gttttacagc attacaaggt aaggaaaaaa
     7321 ttgctatggc taagcaactc caaatacttt gtaagcaata tcaagttcct tttattattg
     7381 atgatgacat tgacttagtt gagttaattg atgctgatgg tcttcatatt ggacaaaatg
     7441 atttacctgt tgatgaggca cggagacgtt taccagataa aatcatcggt ttatctgtga
     7501 gtacaatggc tgaatatcag aaatcacaat tatctgttgt tgattacatc ggaattggtc
     7561 cttttaatcc gacacaatca aaagcagatg ctaagcctgc tgttggcaat aggacaacaa
     7621 aggcagttag agaaattaat caagatattc caatcgttgc aataggcgga atcactagtg
     7681 attttgttca tgatattata gaatcaggcg cagatgggat tgctgttata tcggctattt
     7741 caaaagcaaa tcatattgta gatgcaacca gacaattgcg ctatgaagtt gaaaaagctc
     7801 tggttaatag gcaaaaacac agtgatgtaa tcaaataagg ctacatcgtt aattataaga
     7861 atttttaaga tattatatca tcacacaata ctggtatttg aaaaagaatt taaactatga
     7921 taatatagta gagttgaatt ctagttaaag aaagatttga aagtatgcgt aaaattatta
     7981 ttaacggtgg caagcaactg acgggagaag ttgctgtatc gggtgccaaa aatagtgtag
     8041 ttgccttgat tcctgccact attcttgctg atgatgttgt ggtcttagat ggagttccgg
     8101 ctatttctga tgttgacagt ttagttgaca ttatggaaac tatgggagct aagattaagc
     8161 gttatggtga gacgcttgaa attgatccac gtggcgttaa agatattcca atgccttatg
     8221 gcaaaatcaa tagtcttcgt gcatcctatt atttctatgg aagcttattg gggcgttatg
     8281 gtcaagcaac attaggttta cctggtggtt gtgatttagg cccccgtccg attgatttac
     8341 atttaaaagc ttttgaagcg atgggagcta gtgtttctta tgaaggtgat tcaatgcgtc
     8401 tagcaacaaa cggaaagcct ttgcaaggag caaatattta tatggatact gtcagtgttg
     8461 gtgcgacaat taacactatg atcgcagcag ctaaggcaaa tggtcgtacg gttattgaaa
     8521 atgctgcgcg tgaaccagaa attattgacg tggctactct tttaaataat atgggagctc
     8581 atattcgtgg tgctgggact gatgttatta caattgaagg tgtgaaatcg ctccatggca
     8641 cgcgtcatca agtaattcca gatcgtattg aagcaggaac ttatattgct atggcagcag
     8701 ctatcggacg aggaataaaa gtgaccaatg tgttatatga acaccttgag agtttcattg
     8761 ctaagttaga tgagatggga gttcgaatga cagttgaaga ggattcaatt tttgtcgaag
     8821 aacaagaaaa gttaaaagct gtatccataa aaacctcacc ttacccagga tttgctacag
     8881 atttacaaca acctctaacc cctttattac taacagcaga aggaaacggg agtttattag
     8941 atactatcta tgagaaacgt gttaatcatg ttcccgaatt agcacgtatg ggagctaata
     9001 tttctaccct agggggcaaa atagtttata gtggtcctaa tcaattatct ggtgcacctg
     9061 taaaagcgac agatttgcgt gcaggcgctg ctttagttat cgctggctta atggcagagg
     9121 gacgaactga aattacgaat atcgagttta ttttacgtgg atattctaat atcatcgaga
     9181 aactaacatc attgggtgcg gatatccaat tggttgagga atagtatata taacaaagag
     9241 ttgagaatat ttcttagctc ttttcttctt gttttagatt aagtcactta taggtatttg
     9301 aaatctcttg tggtataata ttatcatgga tatatggaca aatttaggac ggtttgcatt
     9361 cattgaaaca gaacatgtta acctacgtcc tgttgcctac actgataggg aagctttttg
     9421 gcgaatagct tcaaaaagga caaatttaca atttattttt cctgttcaaa ccagtaagaa
     9481 ggaaagtgat tttcttttag ttcattcttt tatgaaagag ccgttgggag tttgggcgat
     9541 tgaagataaa gttagtcata aaatgtttgg agttatccgt tttgaaaaca ttgatttatc
     9601 taaaaagaca gctgaaattg gctatttttt gaaagaaagt tcttggggtc aaggcattat
     9661 gactgaatgt ctaaagacac tttctttttt tgcttttcaa gagtttggga tggacaaatt
     9721 gatcatagta acacataaag agaatattgc tagtcagaag gttgctttga aggcgcattt
     9781 caaacaatct aggagtttta aaggaagcga ccgttataca cgccgtataa gagactatat
     9841 tgagtttcaa ttaactaggg gtgattatcg ttatgagtaa acatcaagaa attttggagt
     9901 acctagaaaa tttagctgtt ggtaagaggg ttagtgtacg cagtatttca aatcatttaa
     9961 aggtgagtga tggcactgca taccgcgcta taaaagaggc agaaaatagg ggtattgttg
    10021 agactagacc gcgtagtggg acagtacgtg ttgctcaaaa agcaaaggtt aatattgaaa
    10081 aactgactta tgctgaaatt gcaaggatta gtgactcaca agttgttgca ggaacagagg
    10141 ggctcagcaa ggagtttagt aaattttcta taggtgcaat gactcacaga aatattgaaa
    10201 aatatttggt gcaaggaggg ttattgattg ttggcgaccg tgatgaaata caacatttag
    10261 ccttacaaca tcaaaacgct atcttagtta caggtggctt taatgtttcc ccatctgttt
    10321 gtcgtttagc agataaactt caaattcccg taatggtaac acactatgat acattcacag
    10381 tatcaacgat gattaatcac actttgtcaa atgccaaaat tcgtactgat ttaaagaccg
    10441 ttgaacaagt ctatcagtcg caaatggatt acggtttctt agcgcaggat gataccgtaa
    10501 aagaatttaa tcttcttgtt aaacaaacta aaaatgttcg ttttccaata gtcaaccaag
    10561 ctaatgttgt cgttggtgtt gttagtgtgc aagatattct tggaaaagat aaagaagtaa
    10621 aattggcaac agtcatgtca aaaaatatca ttgttgctaa acctcgaatg agtctagcta
    10681 atatcagtca aaaaatgatt tttgaagatt tgaatatgat gccagtagta tcggatgact
    10741 ttgaattatt aggcgttatt acaagacgac aagctgtaga aaatttatcg atgtcgcaag
    10801 ggacagcttt atacacctat agtgatcaaa tcttgtctaa tttacaaatt gaagatggtc
    10861 acttttcgtt tttagtagaa cccgctatga ttgaccatac aggtagctta acacaaggag
    10921 ttttgacgga gttcttaaaa gaaatttgta ttcgtgtctt aactcgtaag catcaacgca
    10981 gtattgttgt taaacaaatg acactttatt tcttgcaacc tgttcaaatt gatgaaataa
    11041 taatggtaac gccaacaatt atttcagaaa aacggcgcga agctacactc gatttagaat
    11101 taaaattaga aaataaaatt attgctaaag ctatgatagc ggtaaaaatt aattagatat
    11161 aaggagaata aatagaaaat gattacatta aagtcagcac gcgaaattga agcaatggat
    11221 agagcaggtg attttttagc cagcatccac attggacttc gtgatattat caaaccagga
    11281 gttgatatgt gggaagttga agaatacgtt cgtagacgtt gtaaagaaga aaacgtattg
    11341 ccattgcaaa tcggggttga tggtgcagtt atggattacc catatgctac ttgttgtggg
    11401 cttaatgatg aagtggctca tgcctttcct cgtcattata ccctaaaaca aggtgacctt
    11461 ttaaaagtag atatggtttt aagcgaacct ttagataaat caattgttga tgtttcatcg
    11521 ttaaactttg acaacgtggc tcaaatgaaa aagtacacgg aaacatatag tggtggtttg
    11581 gcagactcat gttgggctta tgctgtaggt gaagtttcac aagaggtgaa agacttaatg
    11641 tctgtgacaa gagaagcaat gtatattggg attgaaaaag cagttatcgg aaaccgtatt
    11701 ggtgatattg gagctgctat tcaggattat gcagagagtc gtggttatgg agttgttcgt
    11761 gatttggttg gtcacggtgt tggtccaaca atgcatgaag agcctatggt gcctaactat
    11821 ggtacagcag gtcgaggtct ccgtcttcgt gaggggatgg ttttaaccat cgaaccaatg
    11881 attaatactg gtacttggga aattgataca gacatgaaaa ctggttgggc acataaaacc
    11941 ttagatggtg gtctttcttg tcaatatgaa catcaatttg ttatcactaa agatggtcct
    12001 gttattttaa cgagtcaagg agaagaaaga acttactaaa atgaaattaa aaaaattctt
    12061 tgaggatttg ctggctaaac tagaatatag acccattcaa gtttttatgc gtcattttca
    12121 aagtgcagag atggacttat cagccatagc agttgcttat tatctattag tgacagcttt
    12181 tccattacta gttattgctg ctaatatttt tccttatttt catattaatg tgtcggattt
    12241 attgtcactt atgcaaaaaa atcttccgaa aaatatttat gaacctgcct cacgcttagc
    12301 agtagatgct ttttcgaaac cgtctaccgg cattttggga ttcgcgtcat taacagcttt
    12361 ttggacaatg tctaaaagtt tgacctctct tcaaaaagcc attaataaag catacggtgt
    12421 tgaccaacac agagactttg tgattagtcg tttagtgggt gtggggactg ggctgatcat
    12481 cctattttta ctgacttttg ttttaatttt ttcaaccttt tcaaaaccag ttttacagat
    12541 aattgtaaac atgtatgatt tgggagatac tctaacggcg tggcttttaa atcttgcaca
    12601 accagttaca tttttgacta tatttttagg tattggaatt ttatacttta ttttacctaa
    12661 cgcacgaatt agaaaagtac gttatgtcat tccagggaca ttattttcaa cgtttgttat
    12721 tggttttttt agtaatttaa ttagccaata cgtcttaaat agggttgaaa aaatggtaga
    12781 tattaaaacg tttggttcgg tcgttatctt tatcttaatg ctatggttta ttttcttagc
    12841 ccacattatg attttaggtg caatactgaa tgccagcgtt caagaaattg cgacaggaaa
    12901 aattgaatca aggcgtggag atattatgtc tcttatccaa aaatccaaag aagaaaaata
    12961 agaaaagaca gctcttgtta gagttgcctt tttttgtctt taaaaataac aaatttgctc
    13021 aagatgtagt tgagtaaaat aattaatatt tgagatacaa ggctttcaat ggtgttgatt
    13081 ttatttaaat tgtgctgaac gaattgtccg ataatagaag gaaattgatc aacaaaaata
    13141 acagctaata tcaaatctaa tcctaatgtg cctaatctag cgatgacaaa tgtttgtaag
    13201 cgttgaaact gtccaatttt agtttgttta aaaacaaatc gatcatttgt aaaaaaagca
    13261 aaaagtatag ctattatgtt agcaactatt gcagagaatg gagcgtcctg cgagaaatta
    13321 aaaatccctt gtcgaataac taaataaaga agggatgtta atagtccgaa cagtaaatat
    13381 aagctgactt cattttgaat actttttttc agtaacgatt ttaatttaac catattttta
    13441 ttatatcacg gatgcttcaa agtgatgagg aaatctccca taagcttggc tttacttttt
    13501 gtttgtgata tacttagtaa gttgttttac acaacccttg atgcttagtt ccctttcatc
    13561 aagcatatta caaacgttag aagtgttaac tgtctaaacg ttaaaggaga aattatgaac
    13621 acatttacaa ctcgcgatta cgctcacatg gctattgtaa cagctatcta tatcgtttta
    13681 accataacgc caccttttaa tgctattgct tatggtgctt atcaattccg tgtttcagaa
    13741 atgctaaatt tcttggcctt ttatcaccga aaataccttt ttgcggtaac gttaggatgt
    13801 atgatttcta atctttatag ttttggaatg attgatgtct ttgtaggtgg tggttcaact
    13861 ttactttttg tttatcttgg aaccatttta ttcaagcaat atcaaaaaga ttatctattt
    13921 aatggcctta ttaacaaagc attctttttc ttttctttct tctttgctgc ttcaatgatt
    13981 acggtagcag ttgaattgaa aattgtagca ggattacctt tattattaac atggttaacg
    14041 acagctgttg gtgaattgac ttcacttttg gttggagcag tccttgtgga taagttgagt
    14101 agacacgtcg attttacaaa ataaatatat tataaagcgc gagacattga tctcggcttt
    14161 tttgtttggt tttatagtta gcatagctag aaaatgctgc aacttttatg agaaatgctc
    14221 caatttttat taatatcctt agtctttgtg ataaaataga aatatggaaa atcgtatgaa
    14281 tgaattagta tctttgttga atcagtacgc aaaagagtat tatacacaag ataatcccac
    14341 agtttctgac agtcaatatg accagttata tcgagagtta gtagaattag aagagcaaca
    14401 tccagaaaac attttgccaa atagtcccac acatcgagtg ggtggtttag ttttagaagg
    14461 ctttgaaaaa taccaacatg aatatccttt gtattcattg caggatgctt tctcaaaaga
    14521 agaattgata gcctttgata aacgtgttaa agcagagttt ccaactgctt cttatatggc
    14581 agagctgaaa attgatggtc tatctgtctc gttgacttat gtaaatggta ttttacaggt
    14641 tggggcaaca cgtggtgatg gcaatattgg agaaaatatt actgaaaatt taaagcgtgt
    14701 tcatgatatt cctctacatt tagaccaaag tttagatatt actgttcgcg gagagtgcta
    14761 ccttcccaaa gaatcttttg aagctattaa catagaaaaa cgtgcaaatg gtgagcagga
    14821 atttgctaat cctaggaatg cagcagcagg aaccttgcgc caattaaaca cagggattgt
    14881 tgcgaaacgt aaattagcaa cttttcttta tcaagaagct agccctacgc aaaaagaaac
    14941 acaagatgat gttttaaaag agctagaaag ctatggcttt tcagtcaatc atcatcgttt
    15001 aattagctct tctatggaga aaatttggga ttttatccaa actatagaaa aagatagaat
    15061 ttccctccct tatgatatcg atggtattgt tattaaggtt aatagcttag caatgcaaga
    15121 agagttgggc ttcactgtaa aggcacctcg ttgggcgatt gcatataaat tccctgctga
    15181 agaaaaggaa gcagaaatat tatctgttga ttggacagtt gggcgaacag gagttgtaac
    15241 accaacagct aacctaactc cagttcaact agcagggaca accgttagta gggccactct
    15301 gcataatgta gattatattg ccgaaaaaga tatccgtatt ggagatactg ttgttgtata
    15361 caaagcaggg gatatcatcc ctgcagtctt aaatgtcgtt atgagtaagc gaaaccagca
    15421 agaagtcatg cttattccta aactatgtcc ttcttgtggt agtgaattag ttcattttga
    15481 agatgaggtg gctttacgtt gtattaatcc gctgtgcccc aaccaaatca aagaacgttt
    15541 agctcatttt gctagccggg atgctatgaa tattacaggt tttggtccat ccttagtcga
    15601 aaaattattt gatgctcatt taatagcaga tgttgcagat atttatcgtt taagtataga
    15661 ggatttatta actttagatg gtattaaaga gaaatctgca actaagattt atcatgcaat
    15721 ccaatcttca aaagaaaatt ctgctgaaaa acttcttttt ggcctaggta ttcgtcatgt
    15781 gggaagcaag gcgagccgcc ttttattgga ggaatttggt aatttgaggc aactaagtca
    15841 agcaagtcaa gaaagtattg cctcaattga tggactagga ggggttattg ccaaatcact
    15901 tcatacgttc tttgaaaaag aagaggtaga taaactacta gaggaattaa cttcctataa
    15961 cgttaatttt aattatctag gaaaacgagt tagtacagat gcacaacttt caggtttaac
    16021 agtcgtttta accggaaaac ttgaaaagat gactcgtaat gaagcaaaag agaaattaca
    16081 aaaccttggt gctaaggtga caggtagcgt ttctaaaaag actgatttaa ttgttgcagg
    16141 aagcgatgca ggaagtaaac taaccaaggc tcaagattta gggattacaa tacaagatga
    16201 agactggtta ctcaaccttt aagtgaggaa gaaatgaaga aacaaaaaaa agcgcgttta
    16261 atttataacc caacttctgg tcaagaaatt atgaaaaaaa atgtagcgga agttcttgat
    16321 atacttgaag gatttgggta tgaaacatca gcctttcaaa caacgccaac taaaaattct
    16381 gccagagatg aagctgctag agctgctcaa gctggttttg atttgattgt tgcagcaggt
    16441 ggagatggta ctattaatga agttgttaat ggtattgctc ctttgaaacg tcgtcctaag
    16501 atggcaatta ttccaactgg aacaactaat gattttgctc gtgccttaaa aataccgcga
    16561 ggtaatccta ttgaagcaac taaattaatt ggtaaaaatc aaatcgttaa gatggacatt
    16621 ggtcaagctc aagaggacaa ttattttatt aatattgcag ctgcaggtag tttgacagag
    16681 ttaacttata gtgtaccaag tcaattgaaa acaacgtttg gctatcttgc ttatctagca
    16741 aaaggtgttg aattattacc tcgagttcga aaagtaccag tcaaaattac tcacgataag
    16801 ggtgaattta tcggtgatgc ttcaatgatt tttgttgcca tcacaaattc tgtaggaggg
    16861 tttgaacaaa ttgcacctga tgccaaattg gatgatggga aatttaccct tattttagta
    16921 aaaactgcaa atcttattga gattatgcat ctcattcgtt tggtgctagc aggtggaaaa
    16981 catattaatg ataaacgagt agagtatatt aaaacgtctt atctaactat cgaaccccta
    17041 tcagacgagc gtatgatgat taacttagat ggagaatatg gaggagatgc tcccattact
    17101 ctagcaaacc ttaaaaatca tattaggttc tttgctaata ctgatgaaat ttcggatgat
    17161 gctttagttt tagataaaga tgaattagcg atagaagcta ttgcacagaa gtttgctaat
    17221 gaggtaaatg atttagaagg attaggagat tgatgaatgg ataatactgt cattgtacat
    17281 taccatagcc gaaagaaaaa ttactttaat ttaagtttat ggcagtggcg cgatggctca
    17341 gagggtcaag atgctcattt ttcacggttt gacagtttcg gtgctgttgc gatactaaaa
    17401 tatcccttgc catatttttt aagccattat tatgtgattg ttaaggataa aaattggcat
    17461 cataaaacaa tagattatcg cattgattgt actaaaggtg gtgcacgtaa agaagtttgg
    17521 attgtagatg gggatgacaa tctgtattat tctcgtcaag cagcagttag tagccatcat
    17581 tatagtagac gccaacctca tgcctacgat atggcaatca atagtagagc gtttgataaa
    17641 aaatggggat ttgatggttg gttaggtttt gaatattcag aaactaaaac ggcttttaga
    17701 ttgtgggccc ctactgcgga acgtgtagaa ctgattttat atcattcgac agatgaaaca
    17761 gcaagtgtat caaaagttct ttctatgaaa agagggacag ccgtcaatta taaaaatcat
    17821 aaagaaaaca cccatggcgt ctggtttact gaacttgaag gaaactataa ttaccaagca
    17881 tatacttatc gagtctacta tagacgacgt acctttaaaa ttacgagaga cccttattcc
    17941 attgcaacga ctgcaaatgg taaaaggtct attgttattg ctccagaagc attaactccg
    18001 gaagggttta aaataagtca tggaaaagaa gctaagtggc ggctcgaaaa ccccaatcaa
    18061 gcagtcattt acgaaatgca tgtgcgtgat ttttcgatat ctgagacttc aggagttaag
    18121 acagattatc aaggaaaatt taagggattg catcaaaaag atacagttaa tcagcatggt
    18181 gataaaacga cctttgatta tgtgcaagac ttaggagtta actatatcca acttcaacca
    18241 attttcgatc atcatcagac gtttgatgat gatggtcact atgcttataa ttggggatat
    18301 gatcctgaaa actacaacgt tccagaagca agcttctcta gtaatccaca tgaacctgca
    18361 acgcgtattt tagaattaaa atctgctatc caagcgtacc atgatgcagg cattggtgtt
    18421 attatggatg ttgtctataa tcatactttt tcatcaactg attctgcatt tcaactaacg
    18481 gttccagatt attattatcg aatgaatcac aatggaacat ttcaaaatgg ttcaggctgt
    18541 ggtaatgaaa cagctagtga aaaagaaatg tgtcgtaagt atattcttga ttcagttttg
    18601 tattgggtga aagaatataa tatcgatggt tttcgatttg atttgatggg actacatgat
    18661 gtagaaacga tgaacattat aagaaatgag ctaaataaaa tcgaccctcg tattttggtt
    18721 tatggagaag gatgggacat gggagctggt ctcacgcctc aaaataaagc taaaaaagat
    18781 aatgcttatc agatgcctgg aattggcttt ttcaatgatg atgtaagaga cgctgtcaaa
    18841 ggtgcggaaa tttacggaga atttaaaaaa ggcttagttt ctggaaacag tacagaagat
    18901 attgttgcaa aaggcatttt aggaagtgat gagttagtat catacattga tcctagtcaa
    18961 gttttaaatt atgttgaagc acatgacaac tacaacttaa atgatttgct atgggaatta
    19021 catcctaatg acaacgagaa acaacatatt taccgagttg aagtagctag tgctatgaat
    19081 ctattgatgc aagggatggc tttcatgcaa ttaggtcagg agtttcttcg aacaaagtgt
    19141 tatcctacag gtgataaagg tcaacttact caagcagata aagaaagagc tatgaattct
    19201 tacaatgccc cagaccaagt taatcaggtc aactgggata atgttacctt tcataagagt
    19261 actattaatt ttattaggaa aataattact ttgaagacaa actctcctta cttttcttac
    19321 tctagctttg aagagattcg caaacatgtt tttgtagagt ctgctcaata ccattcaggt
    19381 ttcataagtt ttacagtcga agaacataag aaaataaaaa taattttcaa tattaaagga
    19441 aaacgcttgc aggatccttt cataggtgat ata
//
LOCUS       DATROY010000019        19306 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.019, whole genome shotgun sequence.
ACCESSION   DATROY010000019 DATROY010000000
VERSION     DATROY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19306
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1023
                     /locus_tag="VBD11_000624"
     CDS             <1..1023
                     /locus_tag="VBD11_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6953595.1"
                     /translation="LILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLL
                     IWAMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNIS
                     SQAVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLP
                     TKLRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVL
                     NMVPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHP
                     ITIMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSE
                     EVKNVE"
     gene            1013..2239
                     /locus_tag="VBD11_000625"
     CDS             1013..2239
                     /locus_tag="VBD11_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953596.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2349..4031
                     /gene="alsS"
                     /locus_tag="VBD11_000626"
     CDS             2349..4031
                     /gene="alsS"
                     /locus_tag="VBD11_000626"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6953597.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4045..4764
                     /gene="budA"
                     /locus_tag="VBD11_000627"
     CDS             4045..4764
                     /gene="budA"
                     /locus_tag="VBD11_000627"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6953598.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(4818..6473)
                     /locus_tag="VBD11_000628"
     CDS             complement(4818..6473)
                     /locus_tag="VBD11_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6953599.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            6832..7836
                     /locus_tag="VBD11_000629"
     CDS             6832..7836
                     /locus_tag="VBD11_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6953600.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            7849..8712
                     /locus_tag="VBD11_000630"
     CDS             7849..8712
                     /locus_tag="VBD11_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953601.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8712..9473
                     /locus_tag="VBD11_000631"
     CDS             8712..9473
                     /locus_tag="VBD11_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6953602.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9770..11431
                     /locus_tag="VBD11_000632"
     CDS             9770..11431
                     /locus_tag="VBD11_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6953603.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11488..12276
                     /locus_tag="VBD11_000633"
     CDS             11488..12276
                     /locus_tag="VBD11_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO6953604.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            12366..13076
                     /locus_tag="VBD11_000634"
     CDS             12366..13076
                     /locus_tag="VBD11_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6953605.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLRNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13314..13985
                     /gene="rpiA"
                     /locus_tag="VBD11_000635"
     CDS             13314..13985
                     /gene="rpiA"
                     /locus_tag="VBD11_000635"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6953606.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            14042..15253
                     /locus_tag="VBD11_000636"
     CDS             14042..15253
                     /locus_tag="VBD11_000636"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6953607.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            15304..15711
                     /gene="arsC"
                     /locus_tag="VBD11_000637"
     CDS             15304..15711
                     /gene="arsC"
                     /locus_tag="VBD11_000637"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6953608.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            15750..16559
                     /locus_tag="VBD11_000638"
     CDS             15750..16559
                     /locus_tag="VBD11_000638"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6953609.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            16561..17817
                     /locus_tag="VBD11_000639"
     CDS             16561..17817
                     /locus_tag="VBD11_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6953610.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            17801..18511
                     /gene="deoD"
                     /locus_tag="VBD11_000640"
     CDS             17801..18511
                     /gene="deoD"
                     /locus_tag="VBD11_000640"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6953611.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            18520..19287
                     /locus_tag="VBD11_000641"
     CDS             18520..19287
                     /locus_tag="VBD11_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6953612.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT         6128 a         3049 c         3947 g         6182 t
ORIGIN      
        1 ctgatattgc cgcttgtaat ttctggcttg ctttattacc tattaaaacc tatggttaca
       61 tttttagaga agcggggaat taagcgtgta acagcgatat tatcagtttt tactattata
      121 atccttctgt taatttgggc aatgtctagt tttattccca tgatgagtaa tcaattacgc
      181 cattttatgg aagatctccc ttcatatgtg aataaagtgc aaatggaaac aagttcgttt
      241 atagatcaca acccttggtt aaaatcttat aaaggggaaa tatcgagcat gttatctaat
      301 atcagtagcc aagcggtctc ttatgctgaa aaattttcaa agaatgtttt agattgggca
      361 ggaaatttag ctagtacagt tgcacgtgtg acagtagcaa caatcatggc tccctttatt
      421 ttgttttatc ttttaagaga tagtcgcaac atgaagaatg gtttcttaat ggttttacca
      481 accaaactac gccaaccagc tgatcgtatt ttgcgagaaa tgaatagtca aatgtcagga
      541 tatgtgcaag gacaaatcat tgttgctatt actgttggtg ttattttttc aataatgtat
      601 agtattatag gccttagata tggcgtgaca ttagggatta ttgccggtgt gttaaatatg
      661 gttccctatt tgggaagttt tgtcgcccaa attccagtgt ttatcttagc gcttgtcgca
      721 ggacctgtta tggttgttaa agttgcgatt gtttttgtta ttgagcaaac tctagaggga
      781 cgctttgtct cacctttggt tttaggtaat aaacttagca ttcatccaat tacaattatg
      841 tttattttat taacctctgg agcgatgttt ggtgtttggg gagtattcct cagtattccg
      901 atttatgcat ctatcaaagt tgttgttaaa gaattgtttg attggtacaa agctgtcagt
      961 gggctatata caatagatgt tgttactgaa gaaagaagtg aagaagttaa aaatgttgaa
     1021 tagtgaaaaa atgattgttt ctatccaaaa tcaggattta gaacatgcta acaagtattt
     1081 cgaaaaagcg ttgaaaaatg atcctgagga aatcttattg gaactaggag cttatttgga
     1141 gagtattggt tttttacccc aagcaaaacg tctctatgat caaatccgac caaattatcc
     1201 agaagttgct atcaatttag ctcaaattgt tgccgaagat ggagatattg aacaagcatt
     1261 cttatactta gattatatct cagaagatag tcaagagtat gtttcagccc tcctcgttat
     1321 ggctgactta tatgatatgg aaggcttgac tgatgttgcg cgtgaaaagc ttttgctagc
     1381 cagcaaactt agcgatgacc cattggttac atttggttta gctgaaatga accttagttt
     1441 ggaacattat caagaagcga tagaaggata tgcatcactt gacaatcgag aaatcttaga
     1501 aacgactgga gtttcaactt atcagcgtat tggtaaatcc tacgctatta tagggaaatt
     1561 tgatgcagct atcgaatttt tagaaaaagc cgttgacatt gagtatgatg atttaacagt
     1621 ttttgagtta gcaacaatcc tttatgatca agaagaatat caaaaagcta acctttattt
     1681 taaacagttg gatactatta atccagactt tgatggctat gaatatatct atggtttatc
     1741 tcttcgtgag gagcataaat ctgaagaagc tttacgtcta gttcagcaag gtcttcgtaa
     1801 aaatagtttt gatggtcaac ttctcttact agcttcacaa ttatcgtatg aattacatga
     1861 tgttccttcg tcggagtctt atttgaaaca agctgaaaaa gtttctgaaa accaagatga
     1921 aattgttatg agattgtcaa atttgtatct tgaagaagag cggtttgagg aagtcttgga
     1981 attagacaat gatgatttag aaaatatcct tgcaaaatgg aatatcgcta aagcacacaa
     2041 ggctttagaa atggatgata gtgtagacta ttatcaatct ttgtataatg atttaaaaga
     2101 taatccagaa tttttacaag attatgctta cattttgaga gaatttggtt accttgataa
     2161 ggcacaagaa gtaggaaagg cttatttaaa attagttcca gacgatatag agatgtcaga
     2221 atgggtgaat aacatttaaa taggaaaaca cttacagaaa aaaataaaaa attcacaagt
     2281 aaattttcac aatctgatgt aaaaactgtg atataattga accattcttg tgaaaagagg
     2341 gagataaaat gacagaatca cataatcaat atggggctga cttaattgtc gatagcctca
     2401 ttaatcatga tgtcaaatat gtttttggta tcccaggagc taagattgac cgcgtatttg
     2461 acactctaga agataaaggt ccagagttaa ttgttgctcg tcatgagcag aacgcgactt
     2521 ttatggctca ggctgtcggg cgtattacgg gagaaccagg agttgttatt gcaactagtg
     2581 gacctgggat ctcgaacttg gcaacagggt tagttaccgc gacagatgaa ggtgatgctg
     2641 ttcttgcaat tggtggacag gttaagcgag gagatttatt gaaacgtgct catcaatcaa
     2701 tgaataacgt tgctatgcta gaacctatta caaaatattc tgctgaggtt catgacccta
     2761 atactttgtc agaaactgtt gcaaacgcct atcgtcttgc taaatctggt aaaccaggag
     2821 caagttttat ctctattcct caagacgtta cagacagtcc agtctcagta aaagcaatca
     2881 aacctcttag tgcacctaaa ttgggttcgg catctgtttt agatattaat tatttagcgc
     2941 aagctattaa caatgctgtt ttaccggtac tgttattagg aaatggcgca tcttcagagg
     3001 gagtaactgc tgccgtacgt cgacttcttg atgctgttaa actaccagta gtagaaactt
     3061 tccaaggtgc gggtattgtg tcacgtgagc tcgaggatga aacattcttt ggtcgtgtag
     3121 gcctcttccg taatcaacct ggagatatgc ttttgaagcg tgcagaccta gttattgcta
     3181 tcgggtatga ccctattgaa tatgaggcgc gtaactggaa tgctgaaatt tctgctcgta
     3241 ttatcgttat tgacgttgaa caagcagaaa ttgatacgta tttccaaccc gagcgagaac
     3301 tgattgggga catggcacat acacttgatt tacttttacc agctattaaa ggatatgaat
     3361 tgccagaagg ttcaaaagag tatttgaaag gtcttcgtaa taacattgaa aacgttagcg
     3421 atgttaaatt tgatcgtgac tcagctcatg gacttgttca tcctcttgat ctcattgatg
     3481 tactccaaga taataccacc gatgacatga ctgttacagt tgatgtggga agccattata
     3541 tttggatggc tcgctacttt aaatcatatg aagcgcgtca cttgctcttc tcaaacggaa
     3601 tgcaaacatt aggagttgct ctaccttggg caatttctgc ggctcttttg cgtcccaaca
     3661 caaaagttat ttcagtttct ggtgatggtg gcttcttatt ctcagctcaa gagttagaaa
     3721 ctgcagttcg tcttcatcta ccaattgttc acattatttg gaacgatgga aaatataaca
     3781 tggttgaatt ccaggaagaa atgaagtatg ggcgttcttc aggtgttgac tttggaccag
     3841 ttgattttgt aaaatacgct gaaagtttcg gtgctaaagg ttaccgtgta gacagcaaag
     3901 acagttttga agaaacactc aaacaagctc taatcgatgc tgaaaatgga ccagtgttga
     3961 ttgatgtgcc tattgactat aaagataacg tgacactagg tgaaacaatc ttacctgacg
     4021 agttctacta agacgaggtc taatatgtca gaaacagtta aattatttca atacagcacc
     4081 ttgagttcac tgatggcagg attgtacaaa ggcagtctaa ctatcggaga attattaact
     4141 cacggtgact taggaatcgg tactgttcat atgattgatg gtgaattgat tgtattggat
     4201 gggaaagctt atcaagctat tggtacagat ggtaaagcag agattatcca attatcagat
     4261 gatgtgacag taccctatgc agcggtttta ccacaccata tccaaaaaca atttgatatc
     4321 aatgctgaga cagataataa ggatttggaa gagatgattc taaaaaactt tgaaggtcaa
     4381 aatctcttta agtctttaaa aatcaaagga acgttttcaa gaatgcacgt tcgtatgatt
     4441 ccaaaatctc cacaacataa acgttttgca gatattgcat ctaatcagcc agaatttacc
     4501 agagagaatg tatctggtac gttggttggg atttggacgc cggaattatt tcatggtgtg
     4561 ggggtaaaag ggttccatgt ccactttatt tcagacgatt taactttcgg tgggcatgtt
     4621 atggattact cgctcacaca aggaaaagtt gaaattggaa aggttgacca acttgaccaa
     4681 tgtttcccaa ctcaagatca agaatttttg aaagctaact ttgacctaca aaagcttaga
     4741 gaagatattg atttaagtga ataagaatac ctataaataa ttaataacac taaaaataga
     4801 cagggatatg tcccagtcta tttttttagt ttaaggctat ctattttttc ttgtgtggga
     4861 gtgactcgta aggttttttg accagtgtaa gttacaaaac ctggcttagt accacttggt
     4921 ttattgagtt tttttgcttc aatcatgtct acttgaacaa gattggagag acgagcttta
     4981 gagtaataag cagctaactc ggcggcatct gtcttaacct cgtcacttgg attgaggtta
     5041 tcccgaatta acacatggct tccggggata tccttggcat gaaaccacaa ctccccttta
     5101 cgagccattt taaatgttaa ctcgtcattt tggagattat tacgtcccac cataataata
     5161 gtcttgccat cggatgctaa atattgttca ggttttttac gcttatgttg tttatcatgt
     5221 gcgcgtcgct taataaatcc agtttcaaca agttcttcac gaatatcatt gatatcttcc
     5281 atagaagcat gatttagcga tgtttcaaca gattcaaggt aggtgattgt atttttcgta
     5341 tctgaaataa ttccttttaa atgttttaca gcttctttta atttttgata ttttttaaag
     5401 tagcgttggg cattttggtt gggtgttaaa gctcgatcaa gtgaaatttc aatcgtttgg
     5461 ttggtgtaat aattatcaag aacaacgata tcttgattat ttggcaccat agagagatag
     5521 gtagttagaa gctctccttt ttggcgaaac tcctctgcat tttcagtagc tagaagttcg
     5581 tcttgttgct tagctagttt tttgatattt ttctctaact cactttgaac acggtgaatg
     5641 aggtcactag cttgctgtgc tatgcgatct ttttcagcct tctcttggta gtaataatct
     5701 aacaattgtc caagagactg aaatgtttcg tgactgcttg agaattggac agcggaaaac
     5761 gatttttctg ttaggctagg ataatattct ctcgaaaaga attgacgaaa atcgttaagt
     5821 ttattgtctt tcaaacagtg agataattct agcgcagtat cgcgtcctaa accttggaag
     5881 agctgttgga ggtttttagg gcttaggtca ttggtttgaa gcagctcaaa tagagtttga
     5941 tcagaaatat caaaaggatt gattgctttt gttttgggtg gggcaatgta agttgaacca
     6001 ggaaggatag tacggtagga gttttgcgag aaaccaacat gcttaattga ttcaataatt
     6061 ttatgttcat ttttatcaat caagataatg ttactgtgtt ttcccatgat ttcaacaaca
     6121 agtgtcgctt taatgtgatc accgatttcg tttttattag acacgatgat ttctaatata
     6181 cggtcatttt caatttgttg aatggtttca atcactgcac cttgcaaata ctttctcatg
     6241 atcattgtaa aagtatttgg attttgagga ttttggaaat ttgcttcggt agtttggata
     6301 cgtccaaata cagggtgagc agataggagt agtttatagt tgcgacgatt attgcgtatt
     6361 gttaatacca attcatggtc aaaaggttgg ttaacttttt ggatacgtcc tttttcaatc
     6421 tgttcttgta attcatttgt gaggtgatgt aaaaaaaatc catcaaaaga catggcgtcc
     6481 cccttattct aaaaattata gcttatttat tataccatag gctgagctaa aaatattttt
     6541 ttataacgca aaaggcttgc aattttcaga atatagtata taattataga aaaattcatg
     6601 agaaaggatg actagtaaat gactaaaaca ttatttacca attggcaaaa ctggcgtaga
     6661 taaagttgtg tttatgggtg catagatacg actttttgga gtacttcaaa aaagtatcta
     6721 tgcgctggtc tagtctaaca atagagagcg caaggtaagt tatttatcag gcgctttttc
     6781 ttttgtgcta aactccagaa agtcaggata ttattaaagt gaggagaaaa tatgaaaaat
     6841 aaaggactga tagctacgct aattctattg accatattag tggttgggga attattttat
     6901 aacaaatcag aaaaacatct taatttatca gaaaagcaag ttgttaaaat tggtatttta
     6961 cagtatgtaa cccatgatgc tttggatgct attgaaaagg gagttgaaga tggtctagct
     7021 caagaaggat ataaagggaa aaaagttaaa ttaaccgttt taaacgccga agctgatcaa
     7081 agtaaaattc aagcgatgag taaacaatta gttaatcatc acaatgatat tttgattgga
     7141 attgcgacac catcagcaca aggtttagca gcttcgacta aggatactcc tataatcatg
     7201 ggagctgttt ctgatccttt aggggccaag ttagtaacga atatgaagaa accaacaact
     7261 aatgttacag gattatcaaa cgttgttccg accaagcaaa ctgtgcaatt aatcaaggat
     7321 ataactccca atgttaaacg tataggaatt ctttacgcta gtagcgaaga taattctgtg
     7381 tcacaagtaa ctgagtttac aaaatatgct caaaaagcag ggctagaagt tcttaaatat
     7441 tctgtaccat ctacaaatga aattaaaaca agtatgtcag tcatgaccaa aaaagttgac
     7501 gctgtctttg ttccacaaga taatactatt gcgagtgctt ttagaacagt tatcgtagca
     7561 gcaaaccaag ctaatatacc tgtgtattcc agtgttgata ccatggttga acaaggaagt
     7621 atagcatcag tagcgcagag tcaatatggc ttagggttag aaactgcaaa acaggccatt
     7681 aaagtcctaa gagggaagcc ggttaaagat gtaccagtca aagttattga tacagggaag
     7741 ccaagcctca atttgaaagc agccaaacac ttaggaatta aaattcctaa gaagattatg
     7801 aaacaggcag aaattaccgt taaggttgac gattagaaag agggttttat gattatttca
     7861 tcagtttcac aaggtttatt atgggggata ttaggattag ggatatactt aacctttaga
     7921 atcttaaaat ttccagacat gacaacagag ggctccttcc ctctaggggg agcggtttgt
     7981 gttaccttaa tgaatcaagg ggttaatcct attttggcga cgattctagg catgctatca
     8041 gggatgttag cgggatttgt tactggttta ctttatacta aaggaaagat accgacgatt
     8101 ttagcaggga ttttagtaat gacctcatgc cactctatta tgctaatggt tatgaaaaga
     8161 gcaaatctag gtttaaatga gattcaaaca ttgaaagatt ttcttccgtt ttcaaatgat
     8221 ttaaatcttc tcgtattagg tttgatagct attctcttgg cgatttctgt cttaatttat
     8281 tttctctata cacgcttggg gcaagcttat attgcaacag gagacaaccc tgatatggca
     8341 aagagcttcg gcattgatac cgacaaaatg gaaatgcttg gcttaattgt atcaaatggc
     8401 ttaattgctt tatcaggagc tttagtaagt caacaagatg gatatgctga tgtatcaaaa
     8461 ggtataggtg ttattgttat tgggttagct agtattatta ttggagaggt actttactct
     8521 actggtttaa ctctctttga acgtttaatt gctatagtgg tgggttctat tctttatcaa
     8581 tttttaataa cggcagtaat cgccttagga tttaatacga attacctaaa attatttagt
     8641 gcaatagtgc ttggaatttg tttaatggtt cctgtcctaa aaacgaagat tttgaaagga
     8701 gtacgcttat aatgatgatg aaaatcatag agttaaaaga agcaaccgta caagtcagta
     8761 atggtttagc agaaatgaaa acgatattag atcatgttaa tttgagtatt tatgaacatg
     8821 acttcattac aatattgggt ggaaatggag ctggaaaatc aacgcttttt aatgtaattg
     8881 caggtacctt gatgttaagc agtggaaata tttacatcat gggacaagat gttactaatt
     8941 tatcagcaga aaaacgagct aaatatttat cacgggtatt tcaagatccg aaaatgggaa
     9001 cagcacctag gatgacagtt gctgaaaatt tattagttgc taaatttcga ggtgaaaaga
     9061 gaccgttagt tcctagaaag attactaact ataccgaaga atttcaaaaa ttgattgcta
     9121 gaactggtaa tggacttgat cgccatttag agacacctac aggtttatta tcaggtggac
     9181 aaagacaagc gcttagttta ttaatggcaa ctttgaaaaa accaaatctg ttattattgg
     9241 acgaacacac agcagcgctt gatcctagaa caagtgtttc gcttatgggc ttgactgatg
     9301 agtttatcaa acaagattcg ttgactgcct taatgattac acatcatatg gaagatgctc
     9361 tcaaatatgg aaatcgtgta ttagtaatga aagatggcaa gattgtcaga gatttaaatc
     9421 aggcacaaaa aaacaagatg gctatagctg actattatca attatttgat taaaaagaag
     9481 tgaaatggaa gtgtttcact tctttttcta tacactttgc aagaaattat tttcaaaata
     9541 tgatatacta ataatgctgt aaatcgactt gtagagacaa ggaccgtaag tacaaatgga
     9601 atgaaaataa ggacacactc tttaattcat attcctatta ttgattaata aggtttttct
     9661 tcaaacccaa gaaaagttac cgataaatca attactaatg ataataattg gacaatttct
     9721 tgtctgcttt ataaaaaaga attaacaaaa tttaaggaga aaactcatta tgagcgacat
     9781 aaaaatcatt gctcttggag gtgttcgtga aaacggaaaa aacctatatg tagtagaagt
     9841 caatgattct atctttgttt tggatgctgg actcaaatat cctgaaaatg aacaactggg
     9901 tgttgatgtt gttattccaa atcttgatta cttaattgaa aataaaaaac gtgttcaggg
     9961 gattttctta acccatggac atgcggatgc tattggtgca ttgccttata ttatcgccga
    10021 agtcaaagca cctgtgtttg gttcacctct cacaattgaa ttagctaagt tattcgttaa
    10081 aaatagtaca gcagtcaaga aattcaataa tttccatgtg attgatagtg aaacggaaat
    10141 tgaatttcaa gatgcggtga tttcgttctt taaaacgact cattcaattc cagagtcaat
    10201 gggaattgtt attgggacaa aagagggaaa tatcgtttac acaggtgact ttaaatttga
    10261 tcaagctgca aggaaatatt accaaacaga tctggcgcgt ttagctgaaa ttggtcgtga
    10321 tggtgtttta gcacttttat cagattctgc aaatgctaca agcaatgagc aagttgctag
    10381 cgaatatgag gtcggtgatg aaattaagag cgttattgaa gacgcagaag gtagagtcat
    10441 cgtagcggca gttgcaagta atctcattcg tattcaacaa gtctttgatg cagcggcaga
    10501 aaacggtcgt cgagtagttt taacgggttt tgatattgaa aatattgttc gtacggccat
    10561 tcgtatgaaa cgcattcata ttgctgatga aaatatgatt atcaaaccta aagatatgac
    10621 acgttacgaa gataacgaat tattaatcct tgaaactgga cgtatgggag agccgataaa
    10681 tggtctccaa aaaatggcga tcggacgtca tcgttatgtt caaattaaag atggtgattt
    10741 agtatttatc gtaacaacac caagtattgc aaaagaggca gttgtagcgc gtgttgaaaa
    10801 tctgatttat aaagctggtg gctcagttaa attgataaca cagaatttgc gcgtatcagg
    10861 tcatgcaaat ggtcgcgaat tacaattatt aatgaatcta ttgaaaccta aatacctttt
    10921 cccaattcaa ggagagtata gggatttgtc agcacatgcg ggcttagctc aagaggtagg
    10981 aatgtcagca gatgatattt atattgtgaa gcgtggtgac atcatggttc ttgaaaaaga
    11041 tggcttcttc catagtggat ctgttcctgc tggagatgtt atgattgatg gaaatgctat
    11101 tggtgacgtt ggtaatattg tacttcgtga ccgcaaggta ttatctgaag atggaatttt
    11161 tattgttgtt ataactgtca gcaaaaaaga aaagaaaatt atttctaaag ctcgtgttaa
    11221 tacgcgtggt tttgtgtatg tgaaaaaaag cagagatatt ttgcgtgaga gcgcagaatt
    11281 agttaataca acggtagaag attacttaag taaagataca tttgactggg gtgaacttaa
    11341 agggaaagtg cgtgatgagg tcagcaagtt cttatttgac caaacgaaac gtcgcccagc
    11401 aatccttcct gtggttatgg aagttcgttg atagtgttaa gaggttacca aattttggta
    11461 acctttcttg ctagaatgga ggtttttatg gcatttttta atattgaata tcactctaag
    11521 gtactaggaa cggaacgtca agtcaatgtt atttacccag atgcttttga gatgtctgat
    11581 gataaaattg atgattgtga cattcctgtt ttgtatcttc tccatggaat ggggggaaat
    11641 gagaattctt ggcaaaaacg cacaaatata gagcgtcttt tacgtcatac taatcttatc
    11701 gtcgtgatgc catcaacaga cttagcatgg tataccaaca caaagtatgg gttggattat
    11761 tttgatgcaa tagctattga gttaccaaaa gttttgaaac gtttctttcc taatatgtct
    11821 gataagagag agaaaaattt cattgccgga ttatctatgg ggggatatgg agcatataaa
    11881 atagctctac tgactaatcg tttttctcat gctgcctctt tgtcaggtgc actttctttt
    11941 gactttgatt tattattcaa taatggcaac gataacacta actattggtc gggaattttt
    12001 ggtgatttga ataacactga taacatagag cgtcattcac taaggcgtta cgttgagtct
    12061 tttgatatga agactaaatt ttatgcatgg tgtggttatg aagattttct ttttgaagct
    12121 aatgaagtcg caattgatga acttcgtcaa ttaggtttaa ctattgacta ttccaatgat
    12181 catggtaaac atgaatggta ttactggaat caacaattag aaaaagtctt agaatggctc
    12241 cctgttgatt atgtcaaaga agagcgcctt tcttagtatt agctaaatcg ttgaaatcca
    12301 agtaaagtct tgttatacta aatcattaag ttttgggaaa ttagacacag aaggtgagat
    12361 aaaaaatgag aacactattt agaatgatat ttgctattcc aaagtttatc tttagattga
    12421 tttggaatat catttgggga atattcaaga cagttcttgt tattgcgatt attttatttg
    12481 gcttgtatta ctatgcgaat cacagtcaat cagaatttgc taatcaactt agtgacatta
    12541 ttcagacagg aaaaacattt ttaaattttg ctgacactaa tcagctcaaa aattcctttg
    12601 ctaatctagc tacggataat gttcatcatt cagaaaatac tagatgggat aaaaatcagg
    12661 caactatcta tatagcaact agagattctg agttggcctc tgcttacaag caagcaatct
    12721 ccaattggaa tgcaacaggt gcttttgtgt tacaaacaac agataatcct aatgctgata
    12781 ttattgctaa ggattactca gatgctaaaa cacaagctgc aggtgtagct gaaaccgaga
    12841 gaaatgcttt aactaatcgt atttcaaagg taacagttaa attaaacaca ttttacctta
    12901 ggaataaaca gtttggttat gaccatacta ggattgttaa tacggccgaa catgaattag
    12961 gacatgcttt aggtctagga cataatgatc aacaacattc cgttatgcag tcaaaaggtt
    13021 cgcattatgg cattcaagaa gttgatattc aaacgctaaa aaacttatat gcataaaaag
    13081 atgagctaat aaactcatct tttttgtttg aacacttatt ttaggtgata taagtgcgtt
    13141 agcaatagat tattaaaaat aaagaatttg actatcacta ctaactaaaa tgcataaaga
    13201 acaaagaaaa tgttttcaaa aatcacttaa aaaatggtat aatgtgtctt gagatagtgt
    13261 tttgaataga cttatcaaaa atattagata acattaactt aaaaggggaa aatatggacg
    13321 aactaaaaaa acttgctgga gtgaccgctg caaaatacgt taaaaacggt atgattgtgg
    13381 gtttagggac aggttctaca gcatattttt ttgttgaaga aattggacgc cgtgtcaaag
    13441 aagagggatt gcaagtcata ggcgttacaa cgtcaaaccg tacgactgag caggcaagag
    13501 gattaggtat tccgttgaaa tcagcagatg atattgatgt cattgatgtc actgtggatg
    13561 gagcagatga agtagaccct gattttaatg gtatcaaggg gggcggtggt gcgctgctca
    13621 tggaaaaaat agtagctact ccaactaaag aatatatctg ggttgtcgat gagagcaaac
    13681 ttgttgaaac gttaggtgca tttaaattgc ctgttgaggt tgttagatat ggttctgagc
    13741 gtctttttcg ggtttttaaa tcaaaaggtt attgtccttc tttccgtgaa acagaaggtg
    13801 accgctttat cactgatatg gggaactaca tcatcgacct agatttgaaa aaaatagagg
    13861 atccaaagca gttagcgaat gaacttgacc atacagttgg tgttgttgaa catggtttat
    13921 ttaatggtat ggttaataaa gttatagtag caagcaaaaa tggtcttgac atacttgaaa
    13981 aataattatt taaataatcg gttttaggag ctaaccgtaa cctccttaga aagaagatat
    14041 aatgtcacaa tttgatcgca ttcacttagt tgtcttagat tcagtgggta ttggagcggc
    14101 accagatgcc aatgattttg tcaatgcagg agttcctgat ggtgcttcag acacgcttgg
    14161 ccacatttca aaaacagttg gacttgcggt gccaaacatg gctaaaatcg gacttggtaa
    14221 tattcctcgt ccccaagcgc ttaagaccgt ccctgcagaa gaaaatccaa gtggctacgc
    14281 gacgaagtta caagaagtat cacttggtaa ggatacaatg acagggcatt gggagatcat
    14341 gggcttaaat attacagagc catttgatac tttctggaat ggttttccag aagatattat
    14401 cacaaagatt gaggattttt caggacgtaa agttattcgt gaagcgaata aaccttattc
    14461 agggacagct gttattgatg acttcggacc acgccaaatg gaaactggtg agctcatcat
    14521 ctatacctca gctgatccag tcctacaaat tgcagctcat gaggatatca tccctctaga
    14581 agaactctac cgtatttgtg aatatgctcg tagtatcaca atggagcgtc cagctctcct
    14641 tggtcgtatt atcgctcgtc catatgtggg tgaaccaggc aactttacgc gtacagccaa
    14701 ccgtcacgac tatgcagtat caccatttga agataccgta ttaaataaac tagatcaagc
    14761 tggcatcgat acttatgctg ttggtaaaat caatgatatt ttcaatggtt caggcattaa
    14821 tcatgatatg ggacataaca aatcaaatag tcatggtatt gatactttga taaaaacgat
    14881 ggggttatca gagtttgaaa aaggcttctc ctttactaat ttggttgact ttgatgctct
    14941 ctatggtcac cgtcgtgatc ctcatggtta tcgtgattgt cttcatgagt ttgatgagcg
    15001 tcttccagag atcgtttcag cgatgcgtga caaagactta cttttgatca ctgccgatca
    15061 tggtaatgac ccaacttatg caggtacaga ccatacaaga gaatacattc ctctcttagc
    15121 ctatagccca tctttcacag gaaacggcct tatcccagta gggcatttcg cagatatttc
    15181 agcaacagtt gcggataact ttggtgttga tactgcgatg attggagaga gcttcttgca
    15241 ggacttggta taaattttta tttccttaag acacttgtag cattaaaaat tgagaggaag
    15301 atgatggaaa aagttaggat ttaccataac ccaaattgtg ggacatcaag gaacgtactt
    15361 gccattattc gtcattgtgg tattgaacca gaaattattt attatctgaa aacaccacct
    15421 agcaagatgg cactagttga actgttgtta gaaatgaaac tttctgtgag agaacttcta
    15481 agaacagatg ttcctgctta tgagaaattc aatttggaat cttctagcgt tacagatgaa
    15541 gaaatgattg atgcgatgat acaagatcct gttcttatca atcgtcccat agttgttacg
    15601 agtaaaggtg caaaactctg ccgaccctgt gaagaaattt taaccatttt acctgtaaag
    15661 atggaaaaag attttgttaa agaagatggg cagattatcc aaagtttata gcatattgta
    15721 tagtgaataa tagtttagga ggtaagtaaa tgacattatt agaaaaaatt aatgagacta
    15781 gagacttttt gcaagcaaaa ggcgtcacag caccagaatt tggtcttatt ttaggctctg
    15841 gtttaggaga attagctgaa gaaatcgaaa atcccattgt tgtggattat gcagacatcc
    15901 caaattgggg acagtcaaca gtagttggtc atgctggaaa attagtgtat ggggatttat
    15961 caggccgtaa ggtattagcg cttcaaggtc gttttcactt ttatgaagga aatacaatgg
    16021 aagtcgttac tttcccagta cgtatcatga gagcattggc ttgccacagt gtgcttgtga
    16081 ctaatgcagc gggtgggatt ggatacggac caggaacttt aatgctgatc aaagaccaca
    16141 tcaatatgat tgggactaac cctctcatag gtgagaacct tgaagaattt ggaccacgtt
    16201 tcccagacat gtcggatgct tatacagcaa catatcgaca aaaagctcac caaattgctg
    16261 aaaaacagaa tatcaaactc gaagaaggtg tgtacttggg tgtatcagga cccacttatg
    16321 aaacacctgc agaaattcgt gcattccaaa caatgggcgc acaagcggta ggtatgtcca
    16381 cggttccaga ggtgatcgtt gcagctcact cagggcttaa agtgttagga atttcagcaa
    16441 ttactaactt tgccgctggc ttccaatcag agctcaatca tgaggaggtc gttgaagtta
    16501 ctcagcgtat taaagaagat ttcaagggat tagttaaatc attagttgct gaactttaaa
    16561 atggtttctc gattatacta tgctgtcaag tttatgatag ccgttctatt tatgacagtt
    16621 atggcaggag ttggagctat cttaatgcac tatgtattaa tgtttacaga gtggttagca
    16681 tttggtgata gtagagaaaa tactctgtct ttgttaaata gtgtcacacc aattaaaaga
    16741 gtgttatcac ttactttagt ctccttttta gcatccctta gttggtatta cttacaaatc
    16801 aagccaaagc aaataactag tattaaacaa caggttgttt ttaaggattt ttctgtaaaa
    16861 aaatcacctt actggctaca tataggtcac gcatttttgc aattgattta tgtaggagct
    16921 ggtggtccta ttggaaaaga aggagcgcca cgagagtttg gagccataaa tgctgggaaa
    16981 atatcggatt tattagcact taaagtgctg gataaacgtt tattaatcgt tagtggtgca
    17041 gctgcaggtt tgtcagcagt ttatcaagta cccttagcta gtgtattctt tgcttttgaa
    17101 actttagctt taggaatttc actgaaaaat attgtgacat tgctagtctc tacttttggt
    17161 gcagctagta ttgctcaatt agttatttcg acagcccctc tttatcatat ttcaaagatg
    17221 agtctgaatt cacagtcatt agcttttatg ttcttgatcg ttttatgtgt aactccgatt
    17281 gcgattagct ttagatattt aaatcaaaaa gtgacagaga gacgtattaa aaatataaaa
    17341 atattgttaa gtttgccagt agtgtcattg atagtatctg ttttatcaat aatctatccc
    17401 cagattttag gaaatggtaa tgccctagtt caagaagtct ttaaagggac cacagttagc
    17461 ttaatcgcta ttttagttgt tctcaagatg atagcaacat tatccactct ttacgctggt
    17521 gcttatggcg gtatactgac accatctttt tccattggtg cttgtttagg ttttttgtta
    17581 gctagtattt ctattccttt acttccccac ctatcgatag taaccagtat gttggttggt
    17641 gcagctattt ttttagctat tacaatgaga gcgccactga cagctgtcgg tttagtcata
    17701 tcttttactg gtcaatctgt tataactatt gttcccctaa ctatagcagt actttttgct
    17761 actgcctatg attattttat aagaaaaatg aggagtttat atgtcaatcc atattgaagc
    17821 aaaacaaggt gaaattgcag ataaaatttt acttccagga gatccactcc gtgcgaaatt
    17881 tattgccgag aattttttag aagatgcggt gtgttttaat acagttcgca atatgtttgg
    17941 ttatacaggt acctataaag gacatcgtgt ttctattatg ggaactggta tgggtatgcc
    18001 ttcaatttca atttatgctc gcgaactaat tgttgattat ggggttaaaa cattgattcg
    18061 cgtaggaaca gcaggtgcta ttaatcctga tatccatgtt cgtgaacttg ttttggcaca
    18121 agcagcagca acaaattcta atattatccg taatgattgg cctgaatttg attttccgca
    18181 aattgctgat tttaaacttt tggataaagc ttatcacatc gcaaaggaaa tggatattac
    18241 aactcatgta ggcagtgtat tatcttcaga cgtgttttat tcaaaccaac cagatcgtaa
    18301 tatggctttg ggtaaactag gtgttcatgc tattgagatg gaagcagcag ctctctacta
    18361 tcttgcagct caacacaatg ttaatgctct tgctatgatg acgatttcag acaacttgaa
    18421 taatccagaa gaagatacaa gtgcagaaga gcgtcaaaca acatttactg atatgatgaa
    18481 agttgggctc gaaacactta tttctgaatg aggatagaaa tgacagattt ttcaactgct
    18541 cttaaagtat tagtggaaca gtattcttat cataatgctt tcttattatt acgaaaacat
    18601 ggtcctttaa actcagattt attattttta ttagaaatga tgaaagagcg tcgagagtta
    18661 aacattgatt ttctttttgc tcatcaagaa caagttgtta tattacaaga aaaatacaat
    18721 ataaagcttt tgcataactc gtatgattta gagttgctgg caaattacat catggattta
    18781 gaagcaaaag taaaaaatgg attaatcata gattttgtcc gtagtgttag tcctatttta
    18841 tatcgtctat ttatgatttt acttgcacaa gaagtacctc acttgcatga ttacatctat
    18901 aatgcaagag atgatcacta cgatacttgg aagtttaaag aattaaagga gtcaaaccat
    18961 ccagtccttt tggcattctc tgaaaggtgg cacgatagtc gcttgacttc taaaagcctt
    19021 gcagaatgtt tacaattaac cgaccttgat gaagaagtga aatcgaccat cattcaatta
    19081 agacagttcg aaaaatcagt cagaaatcct ttggctcacc tgattaaacc ttttgatgag
    19141 caagaactat atcgtacaac tcaattttct tctcaagcat ttttagacca gattatcttc
    19201 ttggcaaagg taattggtgt tgagtatgat actgttaatt ttcactacga tacggttaac
    19261 aagcttatta taaagatact tgagtaattt aaaaacagtt ctgaga
//
LOCUS       DATROY010000020        19122 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.020, whole genome shotgun sequence.
ACCESSION   DATROY010000020 DATROY010000000
VERSION     DATROY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19122
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(41..1261)
                     /locus_tag="VBD11_000642"
     CDS             complement(41..1261)
                     /locus_tag="VBD11_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6953613.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(1448..2815)
                     /locus_tag="VBD11_000643"
     CDS             complement(1448..2815)
                     /locus_tag="VBD11_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6953614.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIAGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(2962..4008)
                     /locus_tag="VBD11_000644"
     CDS             complement(2962..4008)
                     /locus_tag="VBD11_000644"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6953615.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4149..4745)
                     /gene="recR"
                     /locus_tag="VBD11_000645"
     CDS             complement(4149..4745)
                     /gene="recR"
                     /locus_tag="VBD11_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6953616.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(4760..6805)
                     /locus_tag="VBD11_000646"
     CDS             complement(4760..6805)
                     /locus_tag="VBD11_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6953617.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(6934..7626)
                     /locus_tag="VBD11_000647"
     CDS             complement(6934..7626)
                     /locus_tag="VBD11_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6953618.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(7803..8561)
                     /locus_tag="VBD11_000648"
     CDS             complement(7803..8561)
                     /locus_tag="VBD11_000648"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6953619.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(8742..9938)
                     /gene="tuf"
                     /locus_tag="VBD11_000649"
     CDS             complement(8742..9938)
                     /gene="tuf"
                     /locus_tag="VBD11_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6953620.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(10290..11558)
                     /locus_tag="VBD11_000650"
     CDS             complement(10290..11558)
                     /locus_tag="VBD11_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6953621.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
     gene            complement(11664..14459)
                     /gene="ppc"
                     /locus_tag="VBD11_000651"
     CDS             complement(11664..14459)
                     /gene="ppc"
                     /locus_tag="VBD11_000651"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6953622.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            14668..16467
                     /gene="pepF"
                     /locus_tag="VBD11_000652"
     CDS             14668..16467
                     /gene="pepF"
                     /locus_tag="VBD11_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6953623.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(16526..16915)
                     /locus_tag="VBD11_000653"
     CDS             complement(16526..16915)
                     /locus_tag="VBD11_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6953624.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(16899..17423)
                     /locus_tag="VBD11_000654"
     CDS             complement(16899..17423)
                     /locus_tag="VBD11_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6953625.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            17580..18428
                     /locus_tag="VBD11_000655"
     CDS             17580..18428
                     /locus_tag="VBD11_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6953626.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(18461..19078)
                     /locus_tag="VBD11_000656"
     CDS             complement(18461..19078)
                     /locus_tag="VBD11_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO6953627.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
BASE COUNT         6151 a         3686 c         3029 g         6256 t
ORIGIN      
        1 actctttggt aaaaccaaag aattttaaat tagatatata ctacacaact ttagttgtta
       61 attttcttaa atatagagat aacaataatc cgaataagaa taaaaaacca aaaaccatta
      121 atgtcaattg ataggaattt ccccatgaat atgtctttga taacaatagg ggcccaaaca
      181 gacctgctat tgcccatgct gttaaactat aaccatgtaa agtagctaat tcctttgttc
      241 caaaaatatc acttagataa gcaggtaata aggaaaaacc tgcaccataa caagtcatta
      301 aaatagacat cgctattgca aatacaatag cattgaatga caaaaataaa ctagaagtca
      361 taataaagtt cacaataaat aatattataa aggtcaacgg gcgtccaatg tagtcagaga
      421 gacttgccca taacagacgt ccaaaaccgt taaatatccc tagtacccct actaataagg
      481 ctgcagattc tgcggaatag cctgctaaat cttgtgccat tggtgaagct gctgatatta
      541 aacctaagcc acacgaaata ttaataaaca aggtcaacca aatgatgtaa aaggtttttg
      601 attttatagc gacgtttgct tttaatccag tgataatttg tgaattcata gcattctttt
      661 taccatcgtg agtcaaaata gttatttttt cctgaggtgg ttgtttaata aattgtgagg
      721 caatcatcat gacaaaaaaa tatactaatc ccaaaatata aaacgtttta cccacaccaa
      781 tcctaatcag taaggattgt gcaagcggac ttgttactaa agaagcaaag ccaaatccca
      841 taatagcgaa tcctgttgct agtcccctcc tatcaggaaa ccatttaata atagtcgata
      901 ctggagtaat ataacctgaa ccaagtccga ttcctcctaa aataccgtat gcaacataca
      961 gtaaccataa ctgctgagtt tcaatggcta agcctgttaa cacattccct gctccatata
     1021 aaatagcaga aatcattccc attatcctag gaccaaaacg ctctactaag tgtcccataa
     1081 aagctgcaga cattcctaga caaaaaatag ccaaactaaa agcgaatgaa actgatgaaa
     1141 tatcccaacc agtctctgag ataattgggt tacgaaacac actccaagca taagttgatc
     1201 ctagcattaa atgtaaaacg actccagaaa ccgcaactac ataacgattt aaatttttca
     1261 aataatcctc ctaaaaacga acttaagttt tatttttaaa aatatagttc gcaataaaca
     1321 gattatcaaa aaactgaata tatcaccagt tttctgctta gcatatctat tttcctcaat
     1381 aaaactgaat attattcgtg attaaaaaac aaaaaagtta gggtaacttg ttaccctaac
     1441 ccctctatta tcctacttca agaatatcaa ctattttaga aagattcatt gagttagaac
     1501 cttttaataa tatttgatct ttctccttaa gagtatcttt tacttttgca agcaattgat
     1561 caaactgatc cacttcttga ttctttttaa agaaatacac tttaccaatt gggaacatct
     1621 ggcttgctaa ttgagctaac ccctcaatat cctggccata acaaatcaaa gtatcaatag
     1681 agtcaggtct gatactcata atcatttgat tatgtaaatc cacagattgt tctcccagtt
     1741 ctttcatatc agctaaaaga gcaatcttct ttccaccatc attattggga atagctgaaa
     1801 aggtttctag tatcaatcgc atagccgttg ggttagcatt gtaaacatct gataaaatat
     1861 ctgctccatt ggctgatttc ttccattcgg tgcgattacg agttagttga agattttcta
     1921 gagcatccac tatatcttct tctgctactg ctaataattt tccaacatag gcagcgacca
     1981 tcgcattagt tgcattgtac ttaccaggta ctggtatacg taattggtgc tccaaagcat
     2041 tagttttaaa agttaaggaa tgtttttctt ctttgagttc tgtcacttgc aattcttggt
     2101 catggccaaa acgaatagtc atctggtttg ctggaagata tggatcaata attggatctc
     2161 ccggtgcaat taatatgcca tctgaactca taccatctgt gatttgcatt ttcccttctg
     2221 caatcttttc tcgactacca aaaaattcta agtgtgcttc accaatcaaa gtaacaacag
     2281 caatacgtgg ttttgcaatt tcagataaca catggatatc tccaagatga tcctgtccca
     2341 tctccaaaac aattttttca gtatcttctg gcatgtgtag cacagtatat ggtaagccaa
     2401 tctcgttatt gtagtttcct tgtgttttat aagtcttata tgtggttgat aagattgcag
     2461 ctatcatatc tttagttgtt gtcttaccat ttgagccagt tactgcaata acatcaacgt
     2521 tcatcttttc aatataatac tgtgccaaga cttgaaatgc tttaagtgca tcagacacta
     2581 aaagataagg atgacccgct atttccttct ctgaaatggt tgctattgct ccattatcaa
     2641 aagccatctc tataaactca tgtccatctc ttgctccttt taagggaaga aataagtcac
     2701 cttcactgat attacgacta tcaaattcaa tatttcccag gggaacgtct tcaaactctg
     2761 atacttgatt tttagcgcca actactttag caacttcatg caaactaagc ttcatatcat
     2821 ttccttttct attatttagc aacaatgttg tagatagtat gctatccctt ataaacgaat
     2881 atactcctaa attataacac actttcataa catatcatag atttgaaaaa gctcaactaa
     2941 gaaagactta gttgaacttt tttaaattaa atgactctca cgtttttcaa acatttcttt
     3001 agctaacata actaactttt caatcaaatc actataagtt agccccatat tttcccaaag
     3061 cagaggatac attgaccact gagtaaaacc gggcattgta ttcagttcgt ttaagaagat
     3121 ttgtccatct ttcgtcaaaa agaaatcaca gcgtgataaa ccacaagccc cgattgcttt
     3181 aaaagcttta cttgcatatt gacgcattgc ttccatagtt gcttcatcaa ctttagctgg
     3241 aatatccata gtaattttat tatcaatata tttggcgtca tagtcataga aatcgacgtc
     3301 tttaacaact tcgccaggaa aagttgtctt aacatcatta ttgcctaaaa tacctacttc
     3361 aatttcacga gctgtcacgc cttgttcaat caaaatacgg ctatcatact tgagagctaa
     3421 gtcaattgca gagcgaagtg aggattcatc tgtcgctttt gaaataccta ctgatgaccc
     3481 catattagcc ggttttacaa aaattgggaa acttaaagtt tctaaagaga gtttaatcgc
     3541 atgttccaaa tcatcaccct caaaataagt ttgatatgca acctgaggta cacctactgt
     3601 tgcaaggact tgttttgttg taattttatc catagccacg cttgaagata gaatattagt
     3661 cccaacataa ggcatcctta aaacttctaa aaatccttgg atagaaccat cttcccccat
     3721 tggtccatgt aaaacgggga aaacaattgc attatcatca tagatatcac ttggacgaac
     3781 cattttgtct aaatcaacag tttggtttgt cattaacttt tcatctgaag atggcatttc
     3841 atcaaattct tgtgttttaa taaattgacc tacttgcgtg ataaaataag ttttaacaaa
     3901 aaatttatca taattaatag cacgcatgac gctttctgca gacagtacag atacttcacg
     3961 ttccgcagag cgaccaccgt aaagcaagat caatgtttct tttgacatat ttcttctctt
     4021 ttctatctaa tctaagattg accttttaat aataccatat tttaacttaa ctttcaaaaa
     4081 tcaatccact taaagttagt attgtaaaag aaaaagttag gagattccca accttttctt
     4141 tataacaatt ataactctgt acgattttcg attgctcgaa gcaaggttac ttcatctgca
     4201 tattcgatat ctgaaccaac agctaaacct ctcgctaatc gtgtcacttt gataccagct
     4261 ggttttaaga ctcgtgaaat atacattgat gtagcttcac catcagcagt cgcattggta
     4321 gcaacaataa cttctgtcac ctgaccatcc atcaatcgag taattaaact tttcaaatta
     4381 atgtcatcag ggctaatacc attcataggt gaaattaagc catgtaagac atgatataaa
     4441 ccattatact cttgaatttt ttccatagct gacacatctt tactatcttc aacaaccaag
     4501 ataacagact ggtcacgcgt cttatctgta caaattaagc aaggatcatc atcagtcaaa
     4561 ttaccacaaa cagaacaata ggttaattcc cgcttagcag ctaataaatt cttagcaaat
     4621 tcattaacat cttcatcact cataccaatg gtgtaaaaag ctaaacgtgt tgctgtttta
     4681 gtacctatac ccggtaattt tgaaaagctg tcaatcaatt tagcaattgg tgttgggtaa
     4741 agcattcaaa tgtcctctct tattgtcctg tgaactgtga aatatattga tcaatcatat
     4801 ctcgtgcaac taattgatgg gattttgttg tatcagttgt aacatgcgga tacattaccg
     4861 ctactgctat tttacgatta gtatcgtagg caatagcatt taagttgtag gtagaaactg
     4921 tttgtccatt tatattttta gcaaatgttt cagcggtacc agttttacca ctaatggttg
     4981 tcacattccc cctcattgac gtaccagttg catagccact tccactattg acaacgttat
     5041 aaaaaccttc ttgaataatg gctaactctt gatctgagat agctatctta tttagtgttt
     5101 taggagtgat tgaacgaacc aattgagcga acttattaga atcattccct tcatagatat
     5161 ctgaaaccac gtgaggtgct aaacgattcc cattattcgc aatagttgag atatactgac
     5221 caagttgcat tggtgtatag gcatcatact gaccgaacga ttccattaga gttgttccta
     5281 agctgtattt tccaggtaca taaccttctg attcaggtaa atctaatcct gtagaaaccc
     5341 ctaaaccata ttcgccgtaa gttttcctta gtttagccat tgcttcttga tagcctcgat
     5401 cagttaaagc atctcctgta ttatagtctt gtcccattaa acgaagtgct acttgaacca
     5461 tataagtatt agatgaatac tctagcgctt gtgccgcaga tattggagtc aaacctcgtg
     5521 taaaccatga ccgtatatta gcaatttctt ggtcataaag cacttcattt cctctaagaa
     5581 ctttattttc ccatcctgat gatagcgtcg cacctttcac aacagatcct ggcgtgaaat
     5641 tcttagcaat agtgcctagg ctgtcatcaa ctaaattccc atgatcattt ttcagcccag
     5701 ccattgctaa cactttacca gttgaaggtt ctatagcaac tgcatacatc ccttcggaaa
     5761 aactagcgcg cccactagat aattcactgc catagtattg tttcaagata ctttctactt
     5821 tgttttggta attaagatca atcgttaatt taagatttct tcctgactta ccttcttgcg
     5881 taatattatc tgaaacaact ttaccttttt tattaacaac tactttgcga atttgtctga
     5941 ttccttgtaa gtcatcttca tattgttttt caaggtaaga agttcccact cggtcattca
     6001 gtgaatagcc ttttttcaaa tattttttaa cttcttctct tggtaaacct gccttttctg
     6061 tagaaatagt acctaatata tccgataaag aagttccttt tttacgacga tgccaattac
     6121 ttgttacaga aattcctaac agttccttct tatcagcatc cagtttcttg atttgatcat
     6181 cacttaattc acctgtactt aatttcactg aaccaaacgt tggtgtcgca ttcatttggt
     6241 taaataaggc aaccactttt aactcatctt cagaatagtt aatagcgctc actggaactg
     6301 cagcaactgc atttgcatat acggttgatt cggctagatg gttaccaaat ttatcgtaac
     6361 gtttagaatc tggtaaactt tcaacaactt ttttgtagtt ggctttatct gctaaataat
     6421 aatctctctt tgctctatcg cttgaagcaa cttcggttaa ggtaatgtaa tgtgataatt
     6481 tattcgctaa ctctttgata gactgagatg aaatattgtt tcctctagaa aacgccacag
     6541 taggacgttc atcattttca actaagacaa ctcccttagc atcaaaaatc tgacctctcg
     6601 cttttgattc tttcactgta taactcgtaa gagctgtcag ttttttcatg taaaatgatt
     6661 gttgcccaat ctgcatttgc tctaaacgaa gaattagaac agtaaataat aaaacaataa
     6721 taaaaaatag aagatttaat cgtctaggta tgctagcatt ttgctttttt acagtgagac
     6781 gatacctttt tttacgattc aacattaact ccttgctata aaaactctga ctactattgt
     6841 atcataaaaa tggtttttta cagacactca aaatgttata aataataaaa aattcaagcc
     6901 aacttggcct gaatttttta tataaagatt agattattta cctaagtaat attctgaaac
     6961 aaggtttaat ttttcatcaa attcgaaaac aagtggtggg aagttaggaa tttcaacgtc
     7021 catgatttca tcatctgaca attgtttgat atgttttaca agagcacgga ttgagttacc
     7081 gtgtgcacca acaaacacat ttttaccatc tttaagagca ggagcaattt tatcttccca
     7141 gaaaggaaga gcacgctcta aagtaacttt taggttttct gcatctggaa taacagaatc
     7201 atctagtgaa gcatagcgac gatcagtatg tgctgaatgt tcatcatctt tagccatatc
     7261 tggaggcaat acatcatatg aacgacgcca aatatgaact tgctcatcac caaattgttc
     7321 agctgcttct gctttatttt ttcctgtcaa tccaccgtaa tgacgttcgt tcaagcgcca
     7381 tgatttttca actggtaccc aaagttgatc agctgcttca agggcaaggt tagttgtttt
     7441 gatggcacgt ttaagaactg atgtaaaagc aaggtcgaac tcaatacctg ctgcttgaat
     7501 taatttccca gcatcaatag cttgttgtgt acctttttct gaaagatcta cgtcagccca
     7561 tccagtgaaa aggttagctt tattccactc agattcaccg tggcgtgcga atactaattt
     7621 taccattaga aaatttctcc tttaattttg gctataccaa tatttacttt aactattcta
     7681 caaaaaatag ccataaaaat ctagtctgat tactagttca ataatcaaaa atagcttatt
     7741 attaactatc tataagaaaa ggcatatcac ctagagtgct atgcctttag atatcatgtg
     7801 atttatttaa caaaatctaa taaggcaagg aagctttctg cttcaagtga agctccacct
     7861 acaagggcac cgtcaacgtc tgggcaagcc atatattctg caacgttttc aggtttaact
     7921 gaaccaccgt attgaacgcg aactttgtca gcaactgctt gaccgaagtc tgctgcaaca
     7981 acgtcacgaa ctgctttaca catgttttgt gcgtcgtctt gagttgctga tttaccagta
     8041 ccaatagccc agattggttc gtaagcgata acaagtgatg atacttgctc ttctgacaaa
     8101 cctgcaagag ctgctgaaac ttgagcacct acgaattcaa ccgctttacc agcttcgtaa
     8161 gtttcaagag actcaccgca acaaatgatt ggagtcaaac cattagcaaa gatagctttt
     8221 gcttttttgt tgatatcttg gtcagtttca tggaaataat cacgacgttc tgagtgaccg
     8281 ataacaacgt agtctgtacc catttcagcc aatactttag ggctgttttc accagtgaat
     8341 gcacctgaat tctcaaaata gctattttga gcagcgattt tcaattctga acctttagct
     8401 gcttcaagaa ctgttgataa agtaagtgct ggagcagcaa tccctgcttc aacaagttct
     8461 gatgatggta atttagaagc tactgcttcg atgaatgctt ttgcttcttc tggattttta
     8521 ttcattttcc agttaccagc gataaatggt ttacgtgaca ttacacatac ctcttctttt
     8581 tttatttact aatctatcat accataaatt atagttaatt aaaagtttca cgagtctagt
     8641 aaaacgcttt aattatagat actaaaaaag agtccctgag gactcttcac aatttgagct
     8701 gtctgactct aactgccgtt tgggaactta attaagttaa attaagcttc gatttctgaa
     8761 acgatacctg aaccaacagt acgtccacct tcacggattg agaaagtagt accttgttct
     8821 acggcgattg ggtgaatcaa ttcaacttcg atagtaacgt tatcaccagg cataaccatt
     8881 tctgttcctg ctggaagttc gattgaacct gttacgtcag ttgtacggaa gtagaattgt
     8941 ggacggtagt tgttgaagaa tggagtatga cgtccacctt cttctttaga aaggatgtaa
     9001 acttcacctt taaatttagt gtgtgggttg attgaacctg gtttagcaag aacttgacca
     9061 cgttcgattt catcacgttg aacaccacga agaagaacac caacgttgtc ccctgcaaga
     9121 ccttcgtcaa gttgtttacg gaacatttca acaccagtaa caactgcttt ttggatatct
     9181 tctttaatac caacgatttc aacttcgtcg ttgacacgaa cagtaccacg gtcgatacgt
     9241 cctgaagcaa ctgtaccacg tccagtgatt gagaatacat cttcgactgg aagaagtaaa
     9301 ggtttgtcag tatcacgttc tggttctgga atgtactcat caacagtgct catcaattcc
     9361 atgatgatgt cttcgtattt ttcatcgcct tcaagtgctt taagagctga accttggata
     9421 actggaaggt catcacctgg gaagtcgtat tctgaaagaa ggtcacgaat ttccatttca
     9481 accaattcaa gcaattcttc atcatcaaca aggtcaactt tgttcatgaa tacgataagg
     9541 tgtttaacac caacttgacg tgaaagaagg atgtgctcac gagtttgtgg cattggtcca
     9601 tcagttgaag caactacaag gatagctccg tccatttgag cagcaccagt aatcatgttt
     9661 ttaacgtagt ccgcgtgtcc tggagcgtcg atgtgggcat agtgacgttt ttcagtttcg
     9721 tactcaacgt gtgcagtgtt gattgtgatt ccgcgttcgc gctcttctgg agcagcatcg
     9781 atagaagcgt aatcttttgg ttggttaact gaagtaggca agcgacgtgc caatacagtt
     9841 gtgatagctg ctgttaaagt agttttaccg tggtcaacgt gtccgattgt accaatgtta
     9901 acgtgtggtt tactacgatc gtatttttct tttgccattt taggaaaagc ctccaataaa
     9961 atatatttta tagatagacg gtaggcaata cagtctaact ttacttatct attttaacaa
    10021 aatcctctaa aaaagcaagt attttatgct tttttgctta tttctttatt tccttaaaat
    10081 tcgggtaaga ttgattgagg cgtttgttat ctcttatatg tattttttag tagttatcat
    10141 ataatcatat tgttaaatac atcctaattt atagtattag aatattaata gacacttctt
    10201 ttcttcaaat atcttctctc tattaattag agtttaaatt tcaaaaaata caaaaatcga
    10261 gataaaaatc tcgttttttg tttggatgat tatttaaagg catcaaggtt gattattttt
    10321 tcattttttt cttgtggctt atattgctct tctgcttctt tcattatgag ttcctttttc
    10381 tcgttagcat caatattaag cacgaatccg atagctacag aaagtaccaa gaggctgttc
    10441 cctccttggg ataagaaagg aaatgtcact ccagttgagg ggattaaccc agaaatacca
    10501 ccaatgttaa caaaaacttg catcaatagc attgctccaa taccaagagc gatcattgag
    10561 ttgaaaggat ctttagcctt tatacctacg tgcataatac gtaaaattaa gaagaaaacc
    10621 aaagctaaaa taaaccctgc accaataact cctaattcct caataacaat tgaaaagaca
    10681 aagtctgttg ttgcttctgg taagtaccct aatttttcaa tagaattccc aagtcctcga
    10741 ccaaaccagc caccgttgct cattgcataa tatgaattag ccaactggtg tcctgaatct
    10801 gttaaatctt taaatgggtc aaaaaaagca ttaaatcgct tagctacata accaaagaca
    10861 gggactttag ccattgtctc aactccaaca actgcaatag ttccgataaa taaagttgac
    10921 cccacgacga ttagaccaag taaagctgaa aaccatcgat aaccaatacc actaatacag
    10981 tacataataa tgaccgttag aacaattatc gaaccatttc ctaaatcagg ttgtgcaatt
    11041 accaaaccaa tcataaataa agaataaaat cgccaatctt ttaggtcgct tagtgatctc
    11101 ggaagccatc tcccttttgt caaagcttga taatcatata tttctatctt tttttgcctt
    11161 cgagcaaaag taaacgctaa ataccataca atgataactt ttaaatattc agcaggttga
    11221 aaactaatag gaccaataac aatccaacca tgtgctccat taacttcttg tgtgaaaaag
    11281 cgcgctatta gtagcaaaaa tacttctacc aatactgcca tagtcaacac tttagaattt
    11341 ttcaaaaaat ttagtttcag tttatagata aagataattg caaccaaact gactgcccaa
    11401 aagacaccct ggttgattac tgaacgaaaa gggttagctc caagctgaat tagagttgca
    11461 ctagttgtcg aataaattac aattaagcct agaatagaca atatcaaata gggaatgagg
    11521 atggaataat tcagaagatg ccttttatca attttcatat tgtattattt cctttaatat
    11581 atcaaaaaat taccattatg cattatatca tctttcgaag taaagtaaaa gggagaatag
    11641 caatgatcct ccctctatca atattaacct gaatttcgta aacctgttgc aataccatta
    11701 atagtaatat gaatcaactt ctcatagtct tcatctagtt ggttactacg aagacgttta
    11761 atcaattcaa tttgaacata attcaaaaca ttgaaatatg gcaaacgata atctaaactt
    11821 gcatgtaaca ttgggttttc ttctaataaa ttatcatgct gttcaatagc taaaatcata
    11881 tctttagtta actgccattc attaaggatg atattaaaaa catctcgaac ttctttactt
    11941 ccagctaact gagcatactg aagcgcaata ttcatatttg attttgataa aaccatatct
    12001 acattagata aaagagaatt aaagaatggc catttttgat acatggtttg taattttgct
    12061 aagtttgcct catcttgttc aataaattgc ttgaaggctg acccaacacc ataccaaccc
    12121 ggaaacataa tacgattttg tgaccatgaa aatacccatg gaattgcacg taaacctgaa
    12181 atttctgtaa ttgttttacg tgctgctggc cgagaaccaa tatttaaact tgatacctct
    12241 ttaattggac ttgcttcaaa gaaatagtca tagaaataag gattatcaaa aactaaattt
    12301 ctataaacgg cattactttc agccacaata ccatccattg tctcacggaa attatcaatt
    12361 tcattaaggt tagtaatcat acgtgttacc atgcggtcta tggaagctga aataagcatt
    12421 tcaaggttat aataggctgc atcttgatta ccatatttat tctcaatgat ttcaccttgc
    12481 tctgttaggc gaattcgatc tttgattgaa ccaaaaggtt gtgatgtgat agcttcataa
    12541 gatggaccac caccacgacc aacagttcca ccacgaccgt ggaaaaatgt gattttaata
    12601 ccgttttctt cacctatttt tgttagctca ttttgagctt tatatagagt ccaaccagat
    12661 gataaatagc caccatcttt attactatct gaataaccta gcattatttc ttgataattg
    12721 ttgttagttg taatccattt ttttactaat ccataatgta gatactgggt cataatatca
    12781 cgtgaattat ccaaatcttc gatggtttca aataacggta caatctgaat gcgagcttga
    12841 ttggcatcaa ttagtccaac ttcttttaac atgatagcta actcaaacat atccgaaacg
    12901 ctctctgtgt gagaaatgat atgctgtttt ataatgtctt cacccaattg atcttttaac
    12961 tcacgtgctg tttggaaaat agccaattct ttttgaagta actcggattt aggagtatgc
    13021 gtagaagata atgttctagg atcttctgtt aattctttta agagaagttg acatttttct
    13081 tcttctgaaa gagaactgta atcatctaca atattggcag acttcaataa ttctgctaca
    13141 caagcttcat taatactaga atcttgacgc atatcaatag tagctaaata aaatccaaag
    13201 atatctactg cttgaataag acaagctaat tcgccatcga caagagaatc ttgtccatgt
    13261 tgaactaaag attgcttcac aagtaacaaa tcttgtttaa actcctcagc agtagcgtat
    13321 gctggtagtt cttctgtctg tatgtcagaa gctacttgag cgatatgtga ttttatatac
    13381 cttcctacaa taccagaaga ttcttggcga ctaacaaatt tctcttttgg gtaagaacca
    13441 acttttaaat ttagtagggt ttgtactagt ttatcttgga taaaattgaa agctttacga
    13501 taaggttcat tttccctata tactgaatta tcttgtgact ggtttgccaa cgtaacaagt
    13561 tcgggagaga cttcagttag acttattgaa agtgacatat tacgataaag ttcatttagc
    13621 tgctcaatat aatggttaat aatcacttca ctttgtacca tagctgataa tcttagtgtc
    13681 tcagcagtta caaagggatt gccatctcta tcaccaccaa tccacatccc catagtcaga
    13741 ggcttaggat tctctaagtg aatacctttt ttagctgcca gtgccttata ttctgctgtt
    13801 aatttggtaa ctgctttaat aagagaacgg ttgtaatatt ccattacatt ggttatctca
    13861 tttttgactt tcaatttttt ctcgcgaata gtgtctgttt gcatgataat gccgatgtaa
    13921 cgacgcaagt cagcatacca tttttcttga ttcacgattc ctgctttgac atctctgtat
    13981 ttacgtaata aatcatgaat cttagacgtt aattcgagca ccgttttacg ctgcacctga
    14041 gtaggatgag ctgttagcac aggcacaaca ttgacgtgtt caagaatatc tttagcattt
    14101 tcatggccag ctacaacatc aatagtcgtt gataactttc ctaggtaatc ttggttaaga
    14161 ttatttttat aattaatttc atatgctaaa tcaacgtctt ctgaaatatt aattaagagc
    14221 ggcaaaatag agaagtaacg agaaacaata accatctcct cgttgcttaa agccgaaata
    14281 gtttccttca aaccagtata atcgtcagtt gatgataatg aaactatttt ttcaatcttg
    14341 tcaaatgatt cactaccaat caatttttgc gttgcttcat ccaaaagctg cttcagtaaa
    14401 ccgacctctt cggtaatgat ttctttattg ctactacttt ctaatttcgg gtgggacaaa
    14461 ccatcacttc ctctcttaaa tctcactact atgataccat tttaaagtaa aaagtaaacc
    14521 attctaggat ttttagatag aattttcaga tttttactta tttatgacag taatcagttt
    14581 ttcttgttcg acttttttga tatactgagg ataattgaga ttaactgatt aattgttaat
    14641 taacaactat tattaaagga gtatcgcatg gaacttaaaa aacgctctga attccctgaa
    14701 aatgagcttt gggatctgac tgctctctac aaagatcgtc aagatttttt actggctatt
    14761 gaaaaagctc ttgaggatat caaggttttc aaaaaaaatt acgagggaaa acttaattgt
    14821 gttgaggatt ttactagtgc ccttatggaa atcgagcata tttatatcca gatgagtcac
    14881 attgatacct atgcttttat gcctcaaact actgacttta gcaacgaaga gtttgcacaa
    14941 atttctcaag caggatcaga cttcgcaact aaagctaatg tactactttc tttctttaat
    15001 actgccctag caaatgcaga tataaagatt ttagattctt tagaaaataa ccctcatttc
    15061 aaagcaacta ttcgccaagc taaaattcaa aagcaacact tattaagtcc agaagtcgaa
    15121 aaagctttga caaacttaaa tgaagtccta aatacaccat atgatattta tactaaaatg
    15181 cgtgctggcg attttgatat ggaagatttt gaagttgacg gaaaaaccta caaaaatagt
    15241 tttgtaactt atgaaaatta ctttcaaaat cacgaaaatg cagagatacg tgaaaaatcc
    15301 ttccgttcat tctcgaaagg tctacgtaag caccaaaatg ctgcagctgc ggcttatctt
    15361 gcaaaagtca aatctgaaaa gttaattgct gatatgcgtg gttacgatag tgtttttgat
    15421 tatcttcttt ccgaacaaga agttgaccgt tcaatgtttg acagacaaat tgatcttatc
    15481 atggatgaat ttggtccagt agctcaacga ttcctcaaac atatcgccga tgttaatggt
    15541 attgaaaaga tgacttttgc agattggaaa ttagacattg ataacgaact taatcccgaa
    15601 gtaagtatca atgatgctta tgacttagta atgaaatcag ttgctccttt aggaaaagaa
    15661 tattcccaag aggttgagcg ctatcaaaaa gagcgttggg ttgattttgc agctaacgct
    15721 aataaagatt ctggaggata tgctgcggat ccttacaaag ttcatcccta tgtactcatg
    15781 agctggacgg ggcgtatgag cgatgtctac acacttattc atgagattgg acattctggt
    15841 caatttatct tctcagataa tcaccaaagt tttttcaata ctcacatgtc cacttactat
    15901 gttgaagctc catctacttt taacgaactt ttacttagtg actacttaga aaaccaattt
    15961 gatacagcac gtcaaaaacg ctttgctctt gcacatcgct taactgatac ttacttccac
    16021 aatttcatta ctcacttact tgaagctgct ttccaacgta aagtttacac tctaattgag
    16081 gagggagaaa cttttggagc agaacaatta aatgctatta tgaaggaagt attaacacaa
    16141 ttctggggag atgctattga aatagatgac gatgctgctt tgacatggat gagacaagct
    16201 cattactaca tggggctcta tagctatact tactctgctg gtttagtaat ctcgacagcc
    16261 ggctatctca atctcaaaaa taatccaaac ggagcaaaag agtggttagc attcttgaaa
    16321 tctggtggga gctgtacccc tttagaaaca gccctattaa tcggtgctga tatttctact
    16381 gataagcctc tacgcgatac tattaatttc ttatctaaca ctgtagatca aattattaat
    16441 tatagtaatc ttattaaaca aggctaaact acagtctttc aaaaacataa aaacaagttg
    16501 gagatatctc caacttgttt ttatcttatt ttttgtaaac ttcctgataa aattcaattt
    16561 tgtcgttctt tttaacttta atttggtttg ctgccttagg agctaattta ttattgacct
    16621 tgaacatcca ataaagccct ttggtttcat cttggctgat gccatcaatt gaagtaatga
    16681 aaccatcttt ttctttaact ttatagttag ctttaagcac atccaaaaca gtgtcacctt
    16741 tatggtaaac aaccttttca cttaacacgt tcgtatcttc tttaacaacc aattcaacct
    16801 taccaacttc taacgttgag ctagttgttg tcttattcga tgtagatggt tgcccgcaag
    16861 ctactaaagt cgtagcgaat actgttagca agaataattt aatgtatttt ttcattccta
    16921 aatctcctta aaatcggtaa caaaagagga taaaataaac aagttgataa agcgtgtgcc
    16981 atattaaaac taagtcctgc taatacataa gtccaccaag gcatatgata taaccaagca
    17041 aaacaggtat caataatgac gccataaaca acccctaacc ctcctgcaaa gaaagtttga
    17101 agtactacct ccgttatttt accaaaacat acagcttttg tcagcggata tatcagaaat
    17161 ttccacaagc ataaaattaa agtgaaggaa atgatttgca aaaagaccca tggcccaaag
    17221 cccattaaaa aagaagaaac tagcattgtt acagttacag tagctacacc ttcttttaaa
    17281 tcgaataaga caactgtaat caaaaaaatg gcagtaattg gctgaatgtt aggcagaggc
    17341 gcaaaagcat atctcaacac aacacacaaa gcagataata tagctactct agtcaattta
    17401 gttaaagttg aattctttat catgtcttta attatacctc aaaactatga ctaccgctta
    17461 aaagtctttt ttattacaat ttcatttcta atgttacata aacgacctct ttttttaaaa
    17521 gaacgtaaat aagcctgtaa actttgattt tttaaacctt ttaagttaca atatcaataa
    17581 tgagaagaag aattaaacct attgttgttg cggttttttt cagtttattt ggacttttat
    17641 taattattgg tcatttacat tcgacaaata ctttgaaaaa agaattagtt gaagctaaaa
    17701 agacaattcc atccgtaaaa gcttcaaaag taccgcaaaa atcaacatca tcgaaagata
    17761 aagagtttgt tcttaaaccg attatcgatg tctctggttg gcaacttcct aaggagattg
    17821 attacgatac gctttcaaaa aatatttcag gtgttgttat tcgtatcttt ggtggatcaa
    17881 agatatctaa gactaataac gctgcttata caactggaat cgataaatcg tttaagaccc
    17941 atatcaaaga atttcaaaag cgaaatatcc cagtagctgt ctacagttat gcacttggtt
    18001 caagtgttaa agaaatgaaa gaagaggctc agatatttta taagaatgca gctccttaca
    18061 aaccaacttt ttattggatt gacgtagaag aggagacaat gtctaacatg aataaaggtg
    18121 tccaagcatt ccgaaaagag ttaaaaagac ttggtgctaa aaatgttggt atctacatcg
    18181 gtacttactt tatgactgag caaggtatct ctgtaaaagg atttgacgct gtttggattc
    18241 caacttatgg tagcgattct ggatactatg aagcagctcc acaaactgaa cttaaatacg
    18301 atttacacca atacacctct caaggttatc taccaggatt caatcaaccg cttgatttaa
    18361 atcaaattgc agttaataaa gacaagaaga aaacttatga gaaacttttt ggaaaagtaa
    18421 aagattaaaa gctactgtta acagtagctt ttttgataaa ctatttatta tttaattctt
    18481 caatagaaag gattttttga gttggttttt tcctaagcga agtaacgatt gcttctgcca
    18541 ctatcacaac cttagttgga aagacttttt gtacaacaat acctaagaaa ggcaaatgac
    18601 taaataattt tatacacttg gcttggaaaa tcgagagagg tcgctcttca ccatacatca
    18661 aacctggtct tacaaaaaga taatccagac cgcttgcttt gattatctgc tctgccttcc
    18721 ttttactttt aatgtaagct gaatagccgc tattggctga aatgtaaact aactttggta
    18781 tttgattttt gtgacagagt gctactgctt tttgggttgc tttaacgtta agctcatcta
    18841 gttgattggg cttaatcgct ccaatacagt caattaatat atcaaaattt ctatgttcta
    18901 aatgaatctt atcagcttct gtaatatctc ccttaatgta ggttaatcta ggatccttaa
    18961 atatatcacc tttaccttca tgccttgata agtaagccac cttatgccct tttgtaagcg
    19021 ctgcttttat tatctgcttc cctagaaaac cactaccacc tgcaatcagt atttccatag
    19081 ttccctctcc ttcattttgt aaatttagta tagaaatttt ta
//
LOCUS       DATROY010000021        18800 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.021, whole genome shotgun sequence.
ACCESSION   DATROY010000021 DATROY010000000
VERSION     DATROY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18800
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..879
                     /locus_tag="VBD11_000657"
     CDS             <1..879
                     /locus_tag="VBD11_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6953628.1"
                     /translation="RDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGST
                     DGSGNICEKYAMKDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLN
                     YLYNKLRANKSDISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNL
                     KQIFPLLSMKLFRKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFS
                     HTEIKRFTGHDIEKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQSESLNIGDIN
                     LYNKIKQKIALIDYCY"
     gene            1003..2352
                     /gene="gorA"
                     /locus_tag="VBD11_000658"
     CDS             1003..2352
                     /gene="gorA"
                     /locus_tag="VBD11_000658"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6953629.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(2395..2847)
                     /locus_tag="VBD11_000659"
     CDS             complement(2395..2847)
                     /locus_tag="VBD11_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953630.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            3099..4244
                     /locus_tag="VBD11_000660"
     CDS             3099..4244
                     /locus_tag="VBD11_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6953631.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            4246..5460
                     /gene="thiI"
                     /locus_tag="VBD11_000661"
     CDS             4246..5460
                     /gene="thiI"
                     /locus_tag="VBD11_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6953632.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            5562..6740
                     /locus_tag="VBD11_000662"
     CDS             5562..6740
                     /locus_tag="VBD11_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6953633.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    6816..6895
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            6930..7244
                     /gene="rplU"
                     /locus_tag="VBD11_000663"
     CDS             6930..7244
                     /gene="rplU"
                     /locus_tag="VBD11_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6953634.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            7251..7589
                     /locus_tag="VBD11_000664"
     CDS             7251..7589
                     /locus_tag="VBD11_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6953635.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            7611..7904
                     /gene="rpmA"
                     /locus_tag="VBD11_000665"
     CDS             7611..7904
                     /gene="rpmA"
                     /locus_tag="VBD11_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6953636.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            8120..9025
                     /locus_tag="VBD11_000666"
     CDS             8120..9025
                     /locus_tag="VBD11_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6953637.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            9034..9498
                     /locus_tag="VBD11_000667"
     CDS             9034..9498
                     /locus_tag="VBD11_000667"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6953638.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVCVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            9482..10372
                     /locus_tag="VBD11_000668"
     CDS             9482..10372
                     /locus_tag="VBD11_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6953639.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            10548..11069
                     /gene="pyrR"
                     /locus_tag="VBD11_000669"
     CDS             10548..11069
                     /gene="pyrR"
                     /locus_tag="VBD11_000669"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6953640.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            11118..12188
                     /locus_tag="VBD11_000670"
     CDS             11118..12188
                     /locus_tag="VBD11_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6953641.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            12244..13842
                     /locus_tag="VBD11_000671"
     CDS             12244..13842
                     /locus_tag="VBD11_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6953642.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            14044..15288
                     /locus_tag="VBD11_000672"
     CDS             14044..15288
                     /locus_tag="VBD11_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6953643.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            15291..16001
                     /locus_tag="VBD11_000673"
     CDS             15291..16001
                     /locus_tag="VBD11_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953644.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            16016..17263
                     /locus_tag="VBD11_000674"
     CDS             16016..17263
                     /locus_tag="VBD11_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953645.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            17392..17664
                     /gene="rpsP"
                     /locus_tag="VBD11_000675"
     CDS             17392..17664
                     /gene="rpsP"
                     /locus_tag="VBD11_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6953646.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            17674..17916
                     /locus_tag="VBD11_000676"
     CDS             17674..17916
                     /locus_tag="VBD11_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6953647.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            18048..>18800
                     /locus_tag="VBD11_000677"
     CDS             18048..>18800
                     /locus_tag="VBD11_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6953648.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELN"
BASE COUNT         6317 a         2785 c         3737 g         5961 t
ORIGIN      
        1 agggatactg aattaatatc aatcatactt cctgtctata atatgtctgg ttctttatca
       61 aggtgtttag attctatcct taggcaagtt tactcaactt ttgaggtgat cttagtcaac
      121 catggttcta cagatggaag tggtaatatt tgcgagaaat atgcgatgaa ggatgagcgt
      181 attcgtttat ttcatgaaga acatcaagtt attacatctg tatggaactt cgcattagag
      241 aaatctaaag ggaagtatgt tacttttgtg aatccagatg attttcttga tgagtcttac
      301 ttaaattatc tttacaataa attaagagct aataaatctg atatctcagc aaccacctat
      361 acttttttta atgaagagga aaaatgcttt gtttattttg caacagatga taattatttt
      421 gagaaaacat tctttgctca agaatggtta gagcaagaac atagttacca cgataattta
      481 aaacaaatat ttcctctcct ttctatgaag ctctttagaa aatcactttt taacaatatc
      541 acttttcctt tagaagattt aacaggagaa tacacactgt ataatgttta tttgctatca
      601 aggcacatta cattttctca tattggttta tacataacct ctcaagtttt ctctcatact
      661 gaaataaaaa gattcacggg acatgatata gagaagatgc tatctagaaa ggaagagagg
      721 ttagccctgt taacaatggt agggtttaat tctgagaaat acatcaaaaa ttataaggaa
      781 actttattaa attatcaaag tgagtcgttg aatataggtg atattaattt atataataaa
      841 attaagcaaa agattgcatt gatagattat tgttactaga aataatttta acagcaagtt
      901 aggatatgat aatcggtaat ataaattgtt tacgtagcag aaaattgtga ctgtaaacac
      961 tttgtgatag agtataggta tcaatgattc aaggagagag taatgtcaaa acaatatgat
     1021 tatatcgtta ttggtggagg tagtgcaggc agtggtaccg ctaatagggc agccatgtat
     1081 ggagcaaaag tcctgttaat tgaaggtgga caagtaggtg gaacttgtgt taacttaggt
     1141 tgtgtaccta agaaaatcat gtggtatggt gcacaagttt ctgagacact ccataagtat
     1201 agttcaggtt atggttttga agccaataat cttagttttg attttactac tctaaaagct
     1261 aatcgcgatg cttacgtgca gcggtctaga cagtcgtatg ccgctaattt tgagcgtaat
     1321 ggggtcgaaa agattgatgg atttgctcgt tttattgata accatactat tgaagtgaat
     1381 ggtcagcaat ataaagctcc tcacattact attgcaacag gtggacaccc tctttaccct
     1441 gatattattg gaagtgaact tggtgagact tctgatgatt tttttggatg ggagacctta
     1501 ccaaattcta tattgattgt tggggcgggc tatatcgcgg cagaacttgc tggagtggtt
     1561 aatgaattag gcgttgaaac ccatcttgca tttagaaaag accatattct acgcggattt
     1621 gatgacatgg taacaagtga ggttatggct gaaatggaga aatcaggtat ctctttacat
     1681 gctaaccatg tacctaaatc tcttaaacgc gatgaaggtg gcaagttgat ttttgaagct
     1741 gaaaatggga aaacgcttgt cgttgatcgt gtaatatggg ctatcggccg tggaccaaat
     1801 gtagacatgg gacttgaaaa taccgatatt gttttaaatg ataaagatta tatcaaaaca
     1861 gatgaatttg agaatacttc tgtagatggc gtgtatgcta ttggagatgt taatgggaaa
     1921 attgccttga caccggtagc aattgcagca ggtcgtcgct tatcagaaag actttttaat
     1981 cataaagata acgaaaaatt agattaccat aatgtacctt cagttatttt tactcaccct
     2041 gtaattggga cggtaggact ttcagaagca gcagctatcg agcaatttgg aaaagataat
     2101 atcaaagtct atacatcaac ttttacctct atgtatacgg ctgttaccag taatcgccaa
     2161 gcagttaaga tgaagctcat aaccctagga aaagaggaaa aagttattgg gcttcatggt
     2221 gttggttatg gtattgatga aatgattcaa ggtttttcag ttgctatcaa aatgggggct
     2281 actaaagcag actttgatga tactgttgct attcacccaa ctggatctga ggaatttgtt
     2341 acaatgcgct aacgctatca aagaatacaa aaaaagctag ggaacctagc ttttttaagg
     2401 tgttgttgta ttttcaatag ttgaggtatc ggtagtagta ttatttgttt gatcattgtt
     2461 agtactagat gatgtttgat cgttattggc agcagaattt gaactgctac tcggagtact
     2521 actagcagag gaattttcta gtgtttggct ggatgatgcc cttgtctcat ctttttcagt
     2581 agaaacttgg ttactagaat tttgtccact ttctcttata atgacggttt ttgaagtaga
     2641 agaatcagtc ttactagagt ctttgtgatt attaatcgca ttagtaatag taatagaggc
     2701 aacgataata cttaaaacac taccgactaa tgcaattgtt ttttgaagtg cagaaaaacc
     2761 tgtttttata tgcttagcgg gtgattgagt tgaatcagta ttagtcttag tagacttttt
     2821 ctgttgtctt gaatatcctt ttgacatgac gccctcctta aatgtacatc aactattata
     2881 tgattttatc ctaaaaaaag ctaaagaaaa aataatttaa aacatttaaa taagcaacct
     2941 aggttattaa gctaagctat gttagtgtaa taggtagtga gttttgtgat aaattatgag
     3001 aattatcata aatattgttt tgggatgttc acaaactaat ataaatagta ggaaaattat
     3061 gctataataa cactgattga ctttagaagg acagatttat gatttatttt gataattcgg
     3121 caactactat tccctatcca gaagttttaa ggacttatca agaagtagct agtaaaatct
     3181 atggaaaccc ctcaagttta catgagctag gaacaacatc ttctcggatt ttagaggcta
     3241 gccgtaaaca aattgctagt ttacttgagc ttaaagcaaa tgaaattttc tttacctctg
     3301 gtggtacaga ggccgataat tgggttatta agggtcttgc gtttgagaaa caacattttg
     3361 gaaatcatat tattgtttct gatatcgaac acccagctgt taaagagtct gctaaatggc
     3421 ttggagagta tgggtttgag attgattatg cgccagtaga tgataaagga tttgtagacg
     3481 tagaagcact ggtcaaatta attaaaccag aaacgattct tatttcaatt atggcaataa
     3541 acaatgaaat tggttctatt cagccaataa aagctatttc agacttgttg tcagataaac
     3601 ctactatatc gtttcatgtt gatgctgttc aagcaatagg taagataccg actaaagatt
     3661 atttgactga gcgagttgat tttgcatctt tttctagtca taaatttcac ggcgttcgtg
     3721 gggttggctt tctatatatt aaagaaggaa aacgtatttc accattacta acaggtggtg
     3781 gacaagaatc tgatttacgt tcaactactg agaatgtcgc agggattgca gctactgcta
     3841 aagctttacg aatggttatg gacaaggaag tagtagctat tcctaaaata agtaaaatga
     3901 aaaccattat tcatgatgaa ttagccaaat atgaggatat tacgttattt tcgggtaagg
     3961 aaggtttttc acctaacatt atcacgtttg gtatcaaggg tgtccgagga gaagttcttg
     4021 tccatgcctt tgagggacat gatattttta tctcgacaac tagcgcttgc tcttctaaag
     4081 caggtaaacc agcaggaact cttatagcaa tgggaatttc aactaagtta gcccaaactg
     4141 ctgtacgtat cagcttagat gatgataatg atatgggaca agtagagcaa tttttaacta
     4201 tttttaaaca agtttatgaa aaaactaaga aagttagagg ataagatgca gtattcagaa
     4261 attatgattc gttatggaga actctctact aagaagaaaa accgtatgcg cttcatcaat
     4321 aagttaaaaa ataatatgga gcatgtactc tccatttatc cagatgtttc agtaaaaaca
     4381 gatcgtgata gaggacatgt atatctcaat ggtacagatt atcatgaagt tgcagagtcc
     4441 ttaaaagaga tttttggtat ccaagctttt tctccatctt ttaaagtaga aaaaaatgtt
     4501 gatacattgg taaaagctgt ccaggaaatt atgacttccg tttataaaga tgggatgact
     4561 tttaaaatta ccgcaaaacg tagtgaccac tcatttgaat tggatagccg tgctctaaat
     4621 catactttag gagatgccgt tttttcagtc ttgccaaata ttaaggctca gatgaagcaa
     4681 ccagatatca atcttaaagt cgagatacga gatgaggctg cttatatttc atatgaggat
     4741 attaggggtg caggaggatt accggtagga acatctggga aagggatgct gatgttgtct
     4801 ggtgggattg attctccggt agcaggttac ctagcgttaa aacgtggtgt agatatagaa
     4861 gcagtccatt ttgcaagtcc tccttatact agcccaggtg cattgaaaaa agcacatgat
     4921 ttaacacgta aattgacaaa atttggtggt aatattcaat ttattgaagt tccattcaca
     4981 gaaattcaag aggaaattaa ggaaaaagct cctgaagcct acttgatgac gttaacacgt
     5041 aggtttatga tgcgtattac agatcgtatt cgtgaggacc gaaatggtct tgttattatt
     5101 aacggtgaaa gtttagggca ggtggcaagc caaacgctag aaagtatgca agctattaat
     5161 gctgtcactg caacaccgat tattcgtcct gtggtcacga tggataagct agaaattatt
     5221 gatattgctc aaaaaataga tacttttgat atttcaattc aaccatttga ggattgctgt
     5281 acaatttttg caccagatcg cccaaaaact aaccctaaaa ttaagaatac agaacagtat
     5341 gagaaacgta tggatgtaga aggtctggta gagagggcag ttgcagggat tatggtaact
     5401 actattcaac ctcaagcaga tagtgatgat gttgatgact tgattgacga tttattataa
     5461 tgaattttta aactacagat ttactctgta gttttttatt aaaatgattg tgctgtcata
     5521 aggttataaa aaatgctata atacgaataa aggaggcaag tatggcaaga atcccttatc
     5581 aaaaaacact tattttttgt gtagccgtta ttattgctat ttttattttg gggttatcta
     5641 aggatttagc acaatctaaa gggcaaaagg ttgcaaataa taatactgta aaaacagcac
     5701 gtgtggttgc aaacggagat attttgctgc acgatgtact atatgccagt gcgcgccaac
     5761 ccgacggcac ttataatttt actccttatt ttaaagaggt aaaatcttgg attgagagcg
     5821 ctgatttagc gattggtgat tatgaaggga cgattagttc agaatatcct ttagcgggct
     5881 atcctttatt taatgctcct aatgagattg caactactat gaaagaaact ggatatgatg
     5941 tcgttgactt agcacataat catattctgg attcacaact ggcaggtgct atcaatactg
     6001 tcaagacttt taatcgtctg ggattggata ctattggggt ttatttaaag gatagaaata
     6061 aagaagatat cttaattaag cgtgtaaatg gtattaaaat tgctatttta ggttattcgt
     6121 atggctacaa tggtatggaa gctaatgttt caaagagtga ctatgaaaaa catatgtctg
     6181 atttagatac taaaaagatt aagcaagata ttaaaaaggc tgaaaaagaa gcagatatca
     6241 ccattgtcat gccacagatg gggatagaat atcaaaaaaa acctactaca gaacaagtaa
     6301 tactttatca tagtatgatt aaatgggggg cagatattat attcggagga caccctcacg
     6361 ttgttgaacc gtctgaagtg ataaaaaaag atggccagaa aaaatttatt atttactcta
     6421 tgggaaattt tatttcaaat cagcgtttag aaacggttga tgatatctgg acagaaagag
     6481 gattattaat ggatgtaacc atagagaaaa aaggtcaaaa aacagtaatt aaaaaagtta
     6541 aggcgcatcc aacactagta gaagcaaaag caaatggccg ttatgttgaa gaaggctttc
     6601 cagcttatga ttataggacg ctagttttag aggattatat tgaaggtggt aagaaacgtt
     6661 ctacaattga tcagactatg aaagataaag ttgataaagc atataaggaa atgaatgagt
     6721 tagttaattt gaagtggtaa agttgataat tttattttgt tttatcttgc ataaaaaaag
     6781 agtttatgat aaaatttgaa tgttgactaa atgcacatcc tgtgcaaccg cgcgaaaatc
     6841 gttagattaa gtagggcaag gctctcacga tttataggcg agtcttcaca gagggaaacc
     6901 tcacaagaaa tttttatagg aggtgcatca tgagcacata cgcaatcatc aaaactggtg
     6961 gtaaacaagt taaagttgaa gtaggacaag ctatctacgt tgaaaaactt gacgttgaag
     7021 caggagcaga agttacattt aacgaagttg ttcttgttgg tggtgaaaca actaaagttg
     7081 gtactccagt tgttgaagga gctactgttg ttggtactgt tgaaaaacaa ggcaaacaaa
     7141 agaaagttgt ttcttacaaa tacaaaccta aaaaaggtag ccaccgtaaa caaggtcacc
     7201 gtcaacctta tacaaaagtt gttatcaacg caatcaacgc ttaatttttc atgattaaag
     7261 caacttttac tcgtaatcaa tcaggctatc tttatagtgc tgaaatcagc ggtcatgctg
     7321 gtagtggcga gtacggcttt gatgtgattt gtgcagctgt tagtacttta tcaattaatt
     7381 ttattaattc attagaggct ttaacaacct gccaagcgca attgattatc aatgacgttg
     7441 aaggtggtta tatgaagata gatttatctt ctataccaca acataaagag gataaggttc
     7501 aattattatt tgagtcttac cttcttggaa tgactaatct gtctaaagat tcttctgaat
     7561 ttgttagtac agtagtcatg acacactaat ttaagagagg acacaacatt atgttaaaaa
     7621 tgaatcttgc taacttgcaa cttttcgccc acaaaaaagg tggaggttct acatcaaacg
     7681 gacgtgattc acaagcaaaa cgtcttggag ctaaagcagc tgacggacaa acagtatcag
     7741 gtggatcaat tctttaccgt caacgcggta cacacatcta tccaggtgct aacgttggac
     7801 gtggtgggga cgatacactt tttgctaaag ttgaaggtgt tgttcgtttc gaacgtaaag
     7861 gacgcgataa aaaacaagtg tctgtttacc caatcgcaaa ataatgccta gttaagactt
     7921 tatgactatt cataaggtct tttattattg tcagagcttt cattttgtaa caaaagacaa
     7981 tatgttaaga gatttatcat taatactcat aaactttgtt gttcttttat agtgaaatag
     8041 tcctaattca tgaagaattt tccgcaagga tagttatttt ttggtataat gaagggtact
     8101 aaaatgagga agtgataaaa tgaatattca acaactgcgt tatgttgttg ctatcgcaaa
     8161 tagtggtact tttcgtgaag cagctgcaaa actatttgtt agtcagccaa gtttatctgt
     8221 tgccgttcgt gatttggaaa cagaattagg gtttcagatt tttacgcgta caactactgg
     8281 tgcagtgttg accaatcaag gcatgacttt ttatgagaat gctttagaag ttgtgaaaag
     8341 ctttgattca ttcgaaaaac agttttcgca atctgaagct acagagcaag aattctcaat
     8401 agcaagtcaa cattatgatt ttctacctcc tttaatcaca gcgttttcaa aatgtaatga
     8461 taattttagc tatttccgta ttttcgaatc aacaacaata cgtatcttag atgaagttgc
     8521 tcaagggaat agcgaaatag gtatcattta tatcaatagt caaaataaaa aaggtctact
     8581 tcagcggttg gacaaattgg ggttagagtt tgtagagctt attcctttta aaactcatat
     8641 ttatcttggg aaagaccatc ccttggctag taaaacatct ctgataatga cagatttaga
     8701 aggattacca accgtacgtt ttacacagga tagagatgat tatcggtatt actcggagaa
     8761 ttttgtggaa gtacttgata gttctgttac ctataatgtt actgatagag caacattaaa
     8821 tggtatttta gaaagaaccc aagcttatgc gacgggctca ggattcttgg atagccgcag
     8881 tgtgaatggt atcactgtta ttccattaga agatcatcta gataaccaaa tgatttatat
     8941 caaacgaaaa gatcgtaatt tatctcagat ggctttaaaa tttgttgctg ttatggaaga
     9001 gtattttaag ggatttaatt cataggagaa gtaatgagaa agattattat accaataata
     9061 actattttgt tgattgcttt agatcaacta tctaagttat ggattgttaa acatattgaa
     9121 ctaaaccaaa ttaaggaatt cattccaaat atcgttagtc taacttatct gagaaattat
     9181 ggtgctgcat tttctatttt gcaaaatcaa caatggcttt ttaccttaat tacaatcttt
     9241 gttgtttgtg ttgctattat ttatttgatg aaacatatta atggaagtta ttggttatta
     9301 atttctctaa ccttaatcat ctctggtgga ttaggaaact ttatagatcg cttaagatta
     9361 ggttatgtcg ttgatatggt gcatttagat tttattaatt ttgctatctt taatgtagca
     9421 gactcatatc tgacaatcgg tattatttgc ttaatgattg cactttggaa agaggaaagt
     9481 aatggaaatc acaattaaaa tagcaggagt taggctagat aaagctttag ctgataattc
     9541 tgaactgtcg cgtagtcaag ctaatgaaga aattaaaaaa gggattgtgt tagtaaatgg
     9601 acaagtaaaa aaagcgaaat atactgttca agaaggagat cggattactt ttgatattcc
     9661 taaagaggaa gtactagatt atcaagctga aaatatccct ttagatatta tctatcaaga
     9721 tgatgacgtt gctgtggtta ataagccgca agggatggtt gttcaccctt ctgcaggcca
     9781 cagttcgggc accttagtta acgccttgat gtatcatatt aaggatttat catcaattaa
     9841 tggcgttgtt cgcccaggga tcgttcatcg tattgataaa gatacttctg gtttattgat
     9901 ggttgcaaaa aatgatagag ctcaccaagt gttggctgaa gagttgaaag ataaaaaatc
     9961 gcttcgtaaa tatttagcaa ttgtgcatgg taatttacca aatgatcgag gcgtgattga
    10021 ggctccgatt ggtagaagtg ataaagatcg taagaagcaa gccgtgactg ctaaaggtaa
    10081 gccagcaata actcgttttc atgttttaga acgttttggt gattatacct tagttgaatt
    10141 gtcattggag acaggtcgaa cgcaccaaat tcgggttcat atggcttata ttggccatcc
    10201 tctagccggt gatcctgtct acggtccccg caagacttta gggggtaaag gacagttttt
    10261 gcatgcacaa acactaggtt ttacgcaccc tagtaacgga gagaatttga ttttttcagt
    10321 agaggttccg gaaatttttc agacaacttt agacaagttg agaaaaaatt aagtttttct
    10381 tgaaaaatac ttttaccatt tgctataata taagcaaatc attcctttaa ccttatccag
    10441 tgagatagtt aaggagaaac ttcagactaa atcaatgcct gagcggcttt gtcattctag
    10501 agagtctcct tttgaagggg ctcttttatc ttagagagga tactgcaatg aaaagaaaag
    10561 aaattattga tgatgtgacc atgaaacgtg ctattacacg tattacctac gaaattatag
    10621 aacgtaacaa aaatttagat aatattgttt tagcaggcat taaaacaaga ggtgtttttt
    10681 tagccaaacg cattcaagaa cgtttgaagc aattagaaaa tttagatatt cccgttggag
    10741 aattggatac taaacctttc cgtgatgata tgaaagttga agttgacacc acaactatgc
    10801 cagttgacat cactgataaa gatattatat taattgatga tgttctctat acaggtcgca
    10861 ctattcgagc tgctattgat aatcttgtta gtttggggcg tccgagtcgt gtcagtttag
    10921 ctgtattaat tgaccgtggg catcgtgaac tacccattcg tgcagattat gtggggaaaa
    10981 atattccaac aagtcaattt gaagaaattc tagttgaagt gatggaacat gatggttacg
    11041 atcgtgtgag tattattgat ccgagttaag tagacgacta ttatttacga tgattttata
    11101 gaaggggaag aaccacaatg gcaaaaaaat tgttaattct agaggatggt acggtttttg
    11161 aaggactatc ctttggttca tctcttgatg taactggcga attagttttt tgtacgggga
    11221 ataccggcta tcaggaaata ataacgaatc cttcacataa tggaaaaatt ttagttttta
    11281 cctcaccgct aataggaaat tatggtattc atcgtagtta ctctgaagcc attattccca
    11341 cttgtttagg agttgttgtt gcagagtatt ccagatgtgt aagtagcgat actagcaaga
    11401 tgaatctaga tgaatttttg aaaatgaaaa aggttcctgc aatgtcgggg gtcgatacaa
    11461 ggtatttgat gcaagttatt aaagaaaaag gatttgtaaa agctacttta gcagaagcag
    11521 gtgatgtttt atcacattta caagatcaat taatagcgac agtattaccg acaaataatg
    11581 tggaacaagt ttcgacaaaa acagcttatc cgtcgcctgc tagcggtcga aatattgttg
    11641 tacttgattt tggccttaaa cattctgttt tgagagaatt gtctaaacgt cagtgtgacg
    11701 tgactgtcat tccctacaat acgtctttag aaggtatcaa aaatctatac ccagaaggta
    11761 tcatattatc taatggtccg ggaaatccag aaaagcttca ggaagtctta aacactatta
    11821 aagaattaca aaaatctgtt cctatgttag gtattggttt ggggcatcaa ttaattgcca
    11881 tggcaaatgg tgcagaaatt atgagactcc cagttgctaa gaaggggcca aattatccca
    11941 tgagagatat tgctacgggg cgtttagaaa cagttattca gtttaatcat tttaccgtta
    12001 accggctaaa tcttccgcat gatttactgg taacacacga aggtttgaat gatcaagaga
    12061 ttgttgctct aaggcaccgc tcatttccag tgatgtccgt gcaattttac ccagaggctg
    12121 ctccaggccc tcatgatgtg acctattttt ttgatgaatt tttagaaatg attggatcac
    12181 gcgcttaaac ataaggtatt atttaaagtc agtcggtgag ctgattaata aggaggtgcg
    12241 tatatgctaa atacaaagga taaacagcct attttgctta ttattttcgg acagaaaatg
    12301 aaagaatatg acgatttaat tagatcaact gttgagaggt tatctaaaag gaatagacct
    12361 gttagtattc ttatctcgaa ggagctaaac catgtgattc accattatga tgcacaattt
    12421 gaaaatcttg agacgcttgt ggggtatata aatacacatg aagatagttt gatttatccc
    12481 ctttgcttag ataaaaatca tgagctattt caagagtttt tagcgtggca gagtcattat
    12541 tcaaggcagt atatcagtga taatttatca gaaaaaagtg ttcaggcaat agacaataat
    12601 caagactttg tgcaagtaga ttgtttagta atgagagact ctttgaataa ttgtttatat
    12661 gtctctgatt tagaatatat tgaatctaat aaaacaactg gtaagtctct agcgattgta
    12721 ccaagtcaaa ctttatccga tgctgctagg caaacaattc gagatgttgc atttgatgtg
    12781 tgtcgaaagg caaacattat tggagtatgt tactttagtt tcttaataga tttgaacagt
    12841 ttggattatc atataattag cttatcatct ggtttgtctt atcaaagcat tttatttgag
    12901 actattacga cctatcctgt tttagagata gcaactaaat tgacagtggg atatactttt
    12961 agtcaactga agcattctta ttacccaaat acctctgcct ttttagagcc gcaattagac
    13021 tatgtagcta ccgtatcttt tagttttgaa aaagttgact atatcttttt tgctcgtaat
    13081 atagagcaat tatttttaaa tttactcgag gctagcagtc atgatcattt tccatttctg
    13141 agtgatatta gcgaggaaga tttgatgttt gctttgattc agaaaaaaga aaatcgtcta
    13201 gcttatcttt tagaggcttt tagacgtggc tttgatttgt atgatttatc ctcagttact
    13261 aaaatcaacc ctttttactt agataaatgt ttacatattg tagagttgta tgaaaacctg
    13321 aataaaagcc aatataatgt agatatttac aaggatgcta aaaggtatgg tttttcggat
    13381 gactatattg ctagtagttg gcaaatatct ttgatagaca tgttagaata tcgaaaaaaa
    13441 catagtgtcg cacctgttct gaagcaagtc gaacaaagtt ctggtgttct aactggccat
    13501 caaattcagt attttcgaag ctatgattgg cattcagact acagttctag cggttgtcaa
    13561 aaggctttga taatggtaga caagggttat tccctagtaa aattaaatga gttaattaag
    13621 caaatcaaac aaactcattt agaattatta attgttacca atcaacctct cctcatagaa
    13681 caattaaatg atacatctat aatttttgac actataggta ttgagaccat attaacaatt
    13741 atgggtatag aagaggtgga agaagtgtat ttattgggag aaagtcaagc agacatgttt
    13801 tcaaaaatga aaaatttttc tatctcttgt cagtctattt agcaattctt aataaaagct
    13861 gagatataga tctcagcttt tatttgtggt aaaaagtaaa gtactttcaa ttttgtaagt
    13921 tctttctatg agcaaaatag tatacttaat gagtaaaaat attattttta aagaattttc
    13981 ttgacaatat tatttaaaaa taatacactt gttctatatt aataatacaa acaggagatg
    14041 gacatgagta aacgacaaaa tttagggatt agtaaaaaag gagcaattat atcagggctc
    14101 tcagtggcac taattgtagc agtaggtggc tttttatggg tacaatctca acctaataag
    14161 agtgcagtaa aaactaatta caaagttttt aatgttagag aaggaagtgt ttcgtcctca
    14221 actcttttga caggaaaagc taaggctaat caagaacagt atgtgtattt tgatgctaat
    14281 aaaggtaatc gagcaactgt tacagttaaa gtgggtgata aaatcacagc tggtcagcag
    14341 ttagttcaat atgatacaac aactgcacaa gcagcctacg acactgctaa tcgtcaatta
    14401 aataaagtag cgcgtcagat taataatcta aagacaacag ggagttttcc agctatggaa
    14461 tcaagtgatc aatcttcatc atcatcacaa ggacaaggga ctcaatcgac tagtggtgcg
    14521 acgaatcgtc tacagcaaaa ttatcaaagt caagctaatg cttcatacaa ccaacaactt
    14581 caagatttga atgatgctta tgcagatgca caggcagaag taaataaagc acaaaaagca
    14641 ttgaatgata ctgttattac aagtgacgta tcagggacag ttgttgaagt taatagtgat
    14701 attgatccag cttcaaaaac tagtcaagta cttgtccatg tagcaactga aggtaaactc
    14761 caagtacaag gaacgatgag tgagtatgat ttggctaatg ttaaaaaaga tcaggctgtt
    14821 aaaataaaat ctaaggtcta tcctgacaag gaatgggaag gtaaaatttc atatatctca
    14881 aattatccag aagcagaagc aaacaacaat gactctaata acggctctag tgctgtaaat
    14941 tataaatata aagtagatat tactagccct ctcgatgcat taaaacaagg ttttaccgta
    15001 tcagttgaag tagttaatgg agataagcac cttattgtcc ctacaagttc tgtgataaac
    15061 aaagataata aacactttgt ttgggtatac aatgattcta atcgtaaaat ttccaaagtt
    15121 gaagtcaaaa ttggtaaagc tgatgctaag acacaagaaa ttttatcagg tttgaaagca
    15181 ggacaaatcg tggttactaa tccaagcaaa actttcaagg atgggcaaaa aattgataat
    15241 attgaatcaa tcgatcttaa gtctaataag aaatcagagg tgaaataatc gtgacaacta
    15301 atcgaaaaga actgattaga ttgcatcaga ttgttaaaag ttatcaaaat ggggatcaaa
    15361 aacttcaagt cttaaagaac attgatttga cggtttatga aggtgaattt cttgcaatca
    15421 tggggccatc tggctcaggg aaatccacac taatgaatat tattggcttg ttggattccc
    15481 caacttcagg agattatagt ttaaatggta aacgtgtcga agaactaagt caaacaaaat
    15541 tagctcaagt tcgaaataag gaaatcggtt ttgtctttca acaatttttc ttgttatcta
    15601 aactaacggc tttgcaaaat gttgaattac ccttaattta tgcgggtgta gctcctaaaa
    15661 agcgtaaaaa tttagcaaaa caatttttag ataaagttga attgagagag cgtatgaacc
    15721 atctgccaac agaattatct gggggacaga agcaacgtgt agcaatcgcg cgtgctttgg
    15781 tgaatagccc atctattatt ctagcagatg agccgactgg tgccttggat acaaagacgg
    15841 gtgagcaaat tatgcagttc ctaacggaat taaatcaaga aggtaaaacc attatcatgg
    15901 taactcatga accagaaatc gccgattatg cgacacgtaa aattgttatt cgtgatggcg
    15961 aaattacagc agatactact gatagtatta gaattgatta aggggagatt cgattatgga
    16021 aaattggaaa tttgccctat catcgattct agggcacaag atgcgtgctt ttctgaccat
    16081 gttagggatt atcattgggg ttgcatcagt tgtgttaatt atggccttag gtaagggcat
    16141 gaaagattcg gttacaaatg agattacaaa gtctcagaag aatcttcaaa tttattataa
    16201 aacaaaagaa gaccaaaaaa atgaggataa ctttggagct caaggggctt tcatgcaagg
    16261 tagtgatacc aaccgtaagg aaccaattat tcaagaatca tggttaaaaa agattgctaa
    16321 ggaagtagat ggtgttagtg gctactatgt aaccaatcaa acaaatgctc cagtagctta
    16381 ccttgaaaaa aaggcaaaga cggttaatat aacaggaatt aaccgcactt accttggtat
    16441 caaaaagttc aaaattaaaa gtggtcgtca attccaagag gaggattata accaattctc
    16501 gagagttatt ttactagaag aaaaattagc tcagagatta tttcagacaa atgaagcagc
    16561 gttaaataaa gtggtgactg taaaaaataa atcttactta gtagtggggg tttattcgga
    16621 tccagaagct ggctcaggtt tatatggaag taattctgat gggaatgcta ttttaaccaa
    16681 tacacagtta gcttcggagt ttggtgctaa agaagcagaa aacatatatt ttcatcttaa
    16741 tgatgtgtcg caatccaatc gcattggtaa agaaataggc aaacgtttaa cagacattag
    16801 ccatgctaag gatgggtact atgataattt tgatatgaca tctatagtta aatctatcaa
    16861 tacccaagtt ggtattatga caggtgttat tggagcgatt gcagcaattt ctctcctagt
    16921 tggtggtatc ggagtgatga atattatgtt agtctctgta actgagcgca cgagagaaat
    16981 tggccttcgt aaagcccttg gggcaactcg acgtaaaatt ttagctcaat ttttaattga
    17041 atcaatggtc ttaacgattt tgggtgggtt aattgggtta ttactagcct atggaggtac
    17101 gatgttaatt gctaatgcgc aagataagat tactccttct gtgtcactta acgtggctat
    17161 aggcagttta attttctcag catttattgg aattatattt ggtttattac ctgctaataa
    17221 agctagcaaa ttaaacccaa ttgatgcttt acgatatgaa taaaaaaaca tgtactctgt
    17281 caagattttt tcttgacaga gtttgtattt atgttatact tagtaaggta aagttgttag
    17341 ttaagctatc aacgaccaaa tataaagaaa aagaaaagag actatttaaa aatggcagta
    17401 aaaatccgtt taactcgtat gggttctaag aagaaacctt tctaccgtat caatgttgca
    17461 gattcacgtg caccacgtga cggacgtttt attgaaactg ttggaacata caacccatta
    17521 gttgcagaaa accaagttac aatcaaagaa gaacgtgttc ttgaatggtt atcaaaaggt
    17581 gcacaacctt cagatacagt tcgtaacctt ctttcaaaag ctggagttat gactaaattc
    17641 cacgatcaaa aattctcaaa ataataaaag cctatggata ccattgaaaa tctcattatt
    17701 gctattgtga aacctttgat ttcacaacca gatcagttaa ccataaaaat tcaggatggc
    17761 ccagaatttt tagagtacca tcttgattta gatacccaag acatcggtcg tgttatcggc
    17821 aaaaagggtc gtacaattac agcgataagg tcgattgtct attcggtacc aacacaaggg
    17881 aaaaaagtaa gactcatcat tgatgagaaa gaataattta ttctaaaaga taggaacttc
    17941 ctgtcttttt tgttatatct acacaaaaat tgtgagtttt ttagtctttt agaatataat
    18001 ggatgtgaaa gggaattttc tgatttttta ttaaggagga taagatattg ctatttgatt
    18061 ttctcgaaga aaaaataaaa ctgaaagcat acctgatgac cgccttactt gatgatggag
    18121 aaattaacct tgatcacttt aaaacagaat atgctttgag tgaaaagatt ttacaaggtc
    18181 ttatttcgga actacaaata ctttattctg atttctacct tgttgtcaaa agtagaacaa
    18241 tggtattgtt ccattacgaa acactttcta agctagagac tttgcatacc atttatcgag
    18301 agtctaatac gttacagttt ttggcatatt ttattgctcc tcaaaatgtc agtttttctg
    18361 aattcacggc taagaaattc atatccgtcg caagtgccta ccgaataaag aaaaactgta
    18421 aactttattt ggagagtgtt aagttatcaa tacaaaaaaa taaagtgaca ggtccagaat
    18481 atcagattag gctgctgatt gctttgttac agtatcattt tggttttatt atttatcctt
    18541 ttgatgctac aagtgaggta atgataaggg attttattga atactcgaat tttaaagggt
    18601 gtgactacga ccttaaagac ttatctgaag aatattacta tttttatatt ttggtgaatt
    18661 tagtttggaa aagaaaagaa tataaagtag aaattcctca aacagaatcc tttaatcaac
    18721 taaaaaaatc tattctctac atggagttat ccaaagcagc tcaaaagagt attgctatac
    18781 atcttggtat agagttgaat
//
LOCUS       DATROY010000022        18604 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.022, whole genome shotgun sequence.
ACCESSION   DATROY010000022 DATROY010000000
VERSION     DATROY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18604
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            1..621
                     /locus_tag="VBD11_000678"
     CDS             1..621
                     /locus_tag="VBD11_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6953649.1"
                     /translation="MDGVLFDTELFYYKRRERFLKQHGITINHLPMNFFIGGNMKQVW
                     KSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHHKGYLLGLASS
                     STRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIPKQQILIVEDS
                     EKGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            759..1550
                     /locus_tag="VBD11_000679"
     CDS             759..1550
                     /locus_tag="VBD11_000679"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6953650.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            1646..2080
                     /locus_tag="VBD11_000680"
     CDS             1646..2080
                     /locus_tag="VBD11_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6953651.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            2080..3051
                     /locus_tag="VBD11_000681"
     CDS             2080..3051
                     /locus_tag="VBD11_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6953652.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            3109..3333
                     /locus_tag="VBD11_000682"
     CDS             3109..3333
                     /locus_tag="VBD11_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6953653.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            3488..4447
                     /gene="fabK"
                     /locus_tag="VBD11_000683"
     CDS             3488..4447
                     /gene="fabK"
                     /locus_tag="VBD11_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6953654.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            4467..5393
                     /gene="fabD"
                     /locus_tag="VBD11_000684"
     CDS             4467..5393
                     /gene="fabD"
                     /locus_tag="VBD11_000684"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6953655.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYCLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            5402..6136
                     /gene="fabG"
                     /locus_tag="VBD11_000685"
     CDS             5402..6136
                     /gene="fabG"
                     /locus_tag="VBD11_000685"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6953656.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            6152..7384
                     /gene="fabF"
                     /locus_tag="VBD11_000686"
     CDS             6152..7384
                     /gene="fabF"
                     /locus_tag="VBD11_000686"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6953657.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            7350..7886
                     /locus_tag="VBD11_000687"
     CDS             7350..7886
                     /locus_tag="VBD11_000687"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6953658.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            7883..8305
                     /gene="fabZ"
                     /locus_tag="VBD11_000688"
     CDS             7883..8305
                     /gene="fabZ"
                     /locus_tag="VBD11_000688"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6953659.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            8343..9713
                     /gene="accC"
                     /locus_tag="VBD11_000689"
     CDS             8343..9713
                     /gene="accC"
                     /locus_tag="VBD11_000689"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6953660.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            9722..10597
                     /locus_tag="VBD11_000690"
     CDS             9722..10597
                     /locus_tag="VBD11_000690"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6953661.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFIDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            10590..11363
                     /locus_tag="VBD11_000691"
     CDS             10590..11363
                     /locus_tag="VBD11_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6953662.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            11831..12463
                     /locus_tag="VBD11_000692"
     CDS             11831..12463
                     /locus_tag="VBD11_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6953663.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(12509..13786)
                     /gene="serS"
                     /locus_tag="VBD11_000693"
     CDS             complement(12509..13786)
                     /gene="serS"
                     /locus_tag="VBD11_000693"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6953664.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            14085..15146
                     /locus_tag="VBD11_000694"
     CDS             14085..15146
                     /locus_tag="VBD11_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO6953665.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            complement(15184..15546)
                     /locus_tag="VBD11_000695"
     CDS             complement(15184..15546)
                     /locus_tag="VBD11_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6953666.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(15665..16576)
                     /locus_tag="VBD11_000696"
     CDS             complement(15665..16576)
                     /locus_tag="VBD11_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6953667.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            complement(16591..17403)
                     /locus_tag="VBD11_000697"
     CDS             complement(16591..17403)
                     /locus_tag="VBD11_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6953668.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            complement(17436..18446)
                     /gene="manX"
                     /locus_tag="VBD11_000698"
     CDS             complement(17436..18446)
                     /gene="manX"
                     /locus_tag="VBD11_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6953669.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
BASE COUNT         5910 a         2980 c         3878 g         5836 t
ORIGIN      
        1 atggatggtg ttctatttga cactgaatta ttttattata aaaggcgaga gcgtttttta
       61 aaacaacatg gaattacaat taaccattta ccaatgaatt tttttattgg aggaaatatg
      121 aagcaggttt ggaaatctgt tttaggtgat caatatgata cctgggatat tgataagctc
      181 caacaagatt atagtcgcta taaagaggat aatcccttgc catacaaaga tttaattttt
      241 caggattgta aaagagtcat tgaaaagctt catcacaaag gctatctttt gggtctagct
      301 tcaagttcta ctagacatga tatcatgtta gcattagaaa gttttaatct ggatacatat
      361 tttaaggtaa ttctgtctgg tgaggaattt tcagaaagta aacctaatcc agctatttat
      421 aatagagcag cagaactttt agatattcct aaacagcaaa ttcttattgt agaagacagt
      481 gagaagggga ttgcagctgg cattgcagct ggtattgatg tgtgggctat tgaagacaaa
      541 tactttggaa tgaatcagtc gcaggctaat gtattagtga gcgatctgag tcaatttttt
      601 gtgaaattag ataactatta aaaacgggag gtttaattcc gtttttttta ttttttatct
      661 tgccatgaaa gctaggttta tgataaaata caaagcatga taatttgata atcaaattat
      721 tttttatttt tctttcactc ttaaagagag gagacgtttt gaagttcgaa aatataatat
      781 atggtattga tggcagcgtg gccactatta tgcttaatcg accagatatt tcaaatggtt
      841 ttaacatccc aatgtgtcaa gaaattattg atgcaataag gcttatgtct gaaaataaag
      901 atgtcatgtt tttagtgatt gaagcacaag gaccaatctt ttcaatcgga ggagatttaa
      961 aagtgatgaa agatgctgtt gagagcgatg atattagctc attaactaaa attgctgagt
     1021 tagttaatca gatatcgtat gatctactac agctagagaa acctgttgtt atgtgtgtag
     1081 atggggctgt agctggtgcc gcagctaata ttgcattagc agctgatttt gttatcgcaa
     1141 gcaagaagag taaatttatc caggcttttg tgggtgtcgg tttagctcca gatgcaggag
     1201 gtttattgtt acttagtaaa tcaatcggta taactcgtgc agtgcaacta gctttaactg
     1261 gagaaagctt atctgctgaa aaagctgaag ctttgggaat tgtttataaa ctttgtgaat
     1321 cagataaaat aggtaaaatt aaggatcaac tattaaagcg tttaagtaga cattctataa
     1381 actcatatca agccataaaa tcattagctt gggaagctgc ttttaaagat tgggaacaat
     1441 ataaaaagct tgaattacag ttacaagaat cattagcctt taagcaagat tttaaagagg
     1501 gtgttcgggc acacgctgat agaaggcgtc ctaatttttt aggcaaatag tttgaaaatc
     1561 aaattattta agattaaagt cttgcaaata agtttgaact ttgatataat ttgactatca
     1621 aagtttaatg tgaggtggtg atatcttgga atacgatcag attaatagtt atttagttga
     1681 catttttaat agaatcatga ttattgagga aatgagttta aagactagcc aatttagcga
     1741 tgtatcttta aaagaaatgc atactattga aattattggg aaacattctg aggtaactcc
     1801 tagcgatgtc gctcgagaat taatgttaac tttgggaact gtaacaacca gccttaataa
     1861 gttagaaaaa aaaggctaca ttgagcgtaa acggtcaagt attgatcgtc gagttgttca
     1921 cttgagtctt actaagagag gtaggttatt agatcgattg catagtaaat ttcataaatc
     1981 tatggtatct catattattg aagacctagg cgaagaagac ataaaaatgt tgacgagcgc
     2041 tttagggaat cttcataaat ttttggagga cttagtctag tggtttttgc taaaattagt
     2101 caacttgcac attatgcacc ttctcaaatc attaaaaatg aggatttatc attaatcatg
     2161 gataccagtg atgactggat ttcgtcacgt actggtataa aacaacgtca tattagtaaa
     2221 aatgaaacaa cagctgattt ggcaaataag gtcgcagaac aacttattga aaaatccggt
     2281 tattccgcta gtcaaattga ctttattatt gttgcaacga tgacaccaga ctctatgatg
     2341 ccttctactg ccgcaagagt gcaagctcat attggagcga gtaatgcttt tgcctttgac
     2401 ttgagtgcag catgtagtgg ttttgttttt gccttgtcta ccgccgaaaa attgatatca
     2461 tcaggtagct atcaaaaagg tttagtaata ggagcagaaa ctgtttctaa agtattagac
     2521 tggactgata gagggacagc agttttattt ggagatggag ctggaggtgt cttgctagaa
     2581 gcttctaagg aaaaacattt tcttgcagag tctctcaata cagatggtag tcgtcaggga
     2641 cttcaatcta gtcaagtcgg gttaaattca cctttctcag atgaagtact tgatgataaa
     2701 tttttgaaaa tggatggtag agcaatcttt gattttgcta tcaaagaagt ttcaaaaagt
     2761 atcaatcatc ttatagaaac atcccatttg gaaaaagaag atattgatta tcttttctta
     2821 caccaagcga atcgaaggat tttagataag atgtctcgaa aaattgatat agcacgtgat
     2881 aaatttccag agaatatgat ggattatggt aatactagtg cagctagtat ccctatttta
     2941 ctttcagaat cttatgagaa cggtttgttg aaactagatg gtaaccaaac aatcctctta
     3001 tcgggtttcg gtggaggttt gacatgggga agtctaattg ttaaaattta gttatatata
     3061 gtgcatagca catttataaa agattattta aaatttaagg agctaaaaat ggcagtattt
     3121 gaaaaagtac aagaaattat tgttgaagaa cttggtaaag atgcagaaga agtaacgttg
     3181 aacactactt ttgacgatct tgatgctgat tcgcttgatg ttttccaagt gatttcagaa
     3241 attgaagatg catttgatat ccaaattgag acagaagaag gactaaatac tgttggtgat
     3301 ttagttgctt atgttgaaga aaaagtaaaa taattaagtc gtaagaaaaa aagaagcaag
     3361 tgataataaa agatattctt accaaatcac ttattagagt gagtattcaa tcaatcgttg
     3421 atcggtactc actcttcttc ttatcaatat agtttgattg tcaaattatt aattttagga
     3481 aagaaaaatg aaaacacgta taacagaatt actaaatata aaatatccaa tttttcaagg
     3541 gggtatggcg tgggtcgctg atggagatct tgcaggagct gtttctaagg caggtggtct
     3601 aggtattatt ggtggaggaa atgcccctaa agaggttgtc aaagcaaata ttgataaaat
     3661 aaaatctatg actgataaac cctttggtgt gaatattatg cttttatcac cttttgtaga
     3721 tgatattgtt gatttggtta ttgaagaagg tgtaaaagtt gtaacgactg gtgcaggtaa
     3781 tcctggtaag tacatggaac gctttcatga agcaggtata acagtaatcc ctgttgttcc
     3841 tagtgttgca ttagcaaaac gtatggagaa attaggggct gatgctatca ttacagaagg
     3901 aatggaagcg ggtggtcaca tcgggaagct gaccacgatg acattagtgc gacaggttgt
     3961 tgatgctgtt acaattccag tcattgccgc aggtggtata gcagatgggc gtggggctgc
     4021 tgcaggtttc atgttaggag ctgatgctgt tcaggtagga actcgttttg ttgttgctaa
     4081 agaatctaat gctcacccta attacaaagc aaaaatttta aaagcaaaag atatcgacac
     4141 tgctgtttct gctcaggtag taggtcatcc agttcgtgcc ttaaaaaata aactagtaac
     4201 aacctatagc caagcagaaa aagattattt agctggccgt atttcaatca atgagattga
     4261 agaattagga gcgggagctc ttcgcaatgc agttgttgat ggggatgtta ttaatggttc
     4321 agttatggca ggacaaattg caggattaat taaatcagaa gagacttgtc aagagattct
     4381 tgaagatatt tattcaggag cgcgtcaagt tattctttct gaggcaagcc gctggtcaga
     4441 tttataaata caaataagga gacactatga ataaagttgc atttctattt gctggtcaag
     4501 gtgctcaaaa gttaggaatg gctagagatt tatatgaaac tttcccgatt gttaaagaaa
     4561 cttttgataa ggcaagtcat gttttaggat atgatttgag agaattgatt gataaagact
     4621 tagacaaatt aaatcagacg aaatatacgc aaccagctat tttaacgact tcgactgcta
     4681 tttattgttt aattcttaaa gaaattggac ttagaccaga tatggttgca ggactttctt
     4741 tgggagaata tagtgcttta gttgcttcag gtgctataag atttgaagat gctgtagtac
     4801 ttgtttctag acgaggccaa ctaatggaag cagcagcacc tgctggtagt ggcaaaatgg
     4861 tagcggtttt aaatgctgat cgtcaaataa ttgaagatgc ttgtaagaag gcttctcagt
     4921 ttggtattgt tagtcctgca aattataata ctccaaagca aattgttata gggggagagt
     4981 caatcgctgt aaatgcagct gtagaagaac taaaacaaca aggagtaaaa cgtttaattc
     5041 ctctgaatgt ttcaggtcct tttcacactg ctttattgaa acctgcaagt cagaaattga
     5101 gcgatgtttt agataaagtt catttttcag tttctgaaat ccctgtaatt ggaaatacag
     5161 aggcgcagat aatgaaaaaa gacgatatca aatctttatt ggcacgccaa gttatggaac
     5221 ctgtacgctt tgatgagtct attgaaacca tgaaaaaaat gggaatgaca caggttgttg
     5281 aaattggtcc agggaaggta ctctcaggtt tcttgaaaaa aatagatagc tcattatcag
     5341 tatattctgt tgaagataaa attggattta ataatcttaa ggaattaaac taggggaaaa
     5401 tatgcaatta aaagataaga acatttttat tacgggttca agtagaggaa tcggccttgc
     5461 aattgcccat caatttgcac aattaggtgc taatattgtt ttaaatggac gttctgaaat
     5521 atcagaagac ttaatagctg aatttgctga ttatggtgta aaagttattg ctatttcagg
     5581 agatgtttct tcttttgaag atgctaatcg catgattaaa gaagctattg caagtctagg
     5641 tagcgtggat gtgcttgtta acaacgctgg tattactaat gataaattga tgcttaaaat
     5701 gactgttgaa gattttgaga gcgttcttaa aattaattta acaggtgcat ttaatatgac
     5761 acaatcagtc ttgaaaccga tgacaaaagc gcgtcaaggt gctattatta atatctctag
     5821 cgtcgttgga ttaactggaa atgtagggca agctaattac gcagcctcta aagcaggttt
     5881 aattggcttt acaaaatcag ttgccagaga agtagcagca cgaggcattc gtgtcaatgc
     5941 cattgctcca ggctttatcg aatctgatat gacagatgtt atcccagaaa aaatgcaaga
     6001 agctatttta gctcaaattc caatgaaacg tattggaaaa ggtaaagaag tagcgcaggt
     6061 agcgtctttc ttagcagaac aagaatacct tacaggacaa gttattgcca ttgatggcgg
     6121 tatgacgatg caataataaa gaggaaataa tatgacatta cagcgagtag tagtaacagg
     6181 ttacggtgta acctcaccta ttggaaacac accagaagaa ttttggaata gccttaaaga
     6241 aggaaatgtt ggtattggac ctatcactaa atttgattca agtgatttca tggtcaaaaa
     6301 tgctgctgaa attcatgatt ttccatttga taaatatttt gtcaaaaaag atctgaacag
     6361 atttgatatg tattcccttt atgccttata tgcttcaagt gaagctattc aacatgctaa
     6421 tttaaattta gatgaaattg atgcagatcg atttggtgtc atcgttgcat caggtattgg
     6481 agggattcaa gagattgaag aacaagtgat tcgtcttcat gaaaaagggc caaaacgcgt
     6541 aaaaccaatg acgcttccaa aagctctacc aaatatggca gctggaaatg ttgcgatgag
     6601 gttaggcgct catggtgtat gtaaatcaat taatacagct tgtgcttctt ctaatgatgc
     6661 tattggagat gctttccgta atataaaatt tggtattcaa gatattatgg tagtcggtgg
     6721 tgcagaagct gctattacaa aatttgccat tgctggattc caaagtctta cagcattatc
     6781 aacaactgaa gatccatcaa gagcatctat tccatttgat aaagatcgca atggatttat
     6841 catgggagaa ggttctggaa tgttggtctt agaaagttta gaacacgctg aaaaacgagg
     6901 agctactatt cttgcagaag ttgttggtta tggtaacact tgtgacgcat atcatatgac
     6961 ttcaccacat ccagaaggtt taggagccac taaagctatt caacttgcac tggctgaagc
     7021 taatattaaa cctgaagaag ttaactatgt aaatgctcat ggaacttcaa cacctgctaa
     7081 cgaaaaagga gagagtcaag ctgttgttgc tgcacttggg acagacgttc cagtttcttc
     7141 gactaaatct tttactggtc atcttttggg tgccgcagga gctgttgagg caattgcaac
     7201 tattgaagcg atacgtcata gttatgttcc aatgactgct ggaacaacag aattatcaga
     7261 agatattaca gcgaatgtta tttttggtca agggcaagac gctgatattc gttatgccat
     7321 ttcaaataca tttggttttg gaggacacaa tgcagttctg gccttcaaac gctgggagga
     7381 ttaaattgga tattcaagag attaaggatt taatgactca atttgatgag tcaagtctga
     7441 gagaattttc attcaaaaca agtgacggtg agctgtcttt tagtaaaaat gagggaaaag
     7501 ctccacttgt accaactatg tctcctatga gtcatcaacc tgaagcgact ccaactatag
     7561 caactcctgt ctctaatgaa gcaggtgagc aaacgaaaca agctactgaa gtagtttcag
     7621 aaatcccaga gtcgactgtg actgttgcag aaggtgacgt tgtcgaaagt cctttagttg
     7681 gggttgctta tttagcttct ggaccagata aacctaactt tgtttcagta ggtgatagcg
     7741 tgaagaaggg acaaactttg atgattattg aggcaatgaa ggtgatgaat gaagtgcctg
     7801 cccctcatga tggtgtagtt actgaaattc ttgtagccaa tgaagaagtt attgaatttg
     7861 gtaaaggatt ggtacgaatc aaatgattga tataaaagaa attcgtgagg ctttacctca
     7921 ccgctatcca atgttattag tggatcgtgt tttagaggta tcagaagatg agatagtcgc
     7981 tatcaaaaat gttagtatca atgagccttt tttcaatggt cattttccag aatatcctgt
     8041 aatgccaggc gttttaatta tggaagcttt ggctcaaact gcaggtgttt tagaattatc
     8101 taaagaagaa aacaaaggaa agcttgtttt ttatgcaggt atggataaag tgaaatttaa
     8161 aaagcaagtt gttccaggag atcaattagt tatgactgct aagtttgtca aacgtagagg
     8221 aactatagcc gttgttgaag caatagcaga agttgatggg aaattagctg ccagtggaac
     8281 gttaacattt gcaataggta attgaagata gtattgaatt tagagggaga gaagcatcaa
     8341 aaatgtttaa gaaaatattg attgctaatc gcggagaaat tgcagtacgt atcattcgtg
     8401 cagcccgtga aatgggaatt tcgacagttg ctatctattc ggaggctgac aaggaatcat
     8461 tgcacacaat tttagcagat gaagctattt gtgttggacc agccaaatca gcagaatctt
     8521 atttaaacgt taatgctata ttatctgctg ctattgtgac tggagcggaa gctgttcatc
     8581 caggttttgg gtttttgagt gaaaattcta aatttgctac tatgtgtgaa gagatgaact
     8641 taaaattcat tggtccatct ggagaagtta tggataaaat gggtgataaa atcaatgctc
     8701 gtgcagagat gataaaagct gatgttccgg ttatcccagg atcagatggt caagttacaa
     8761 gtgtagaaga agctgtatca attgctgagg aaatcggcta tccattgatg ttaaaagcct
     8821 cagccggtgg cggtggaaaa ggaattagga aggtcaaatc agcagatgaa ttgaagcctg
     8881 cttttgagtc tgcttcacag gaggctttgg ctgcgttcgg taatggtgct atgtatattg
     8941 aaaaagttat atacccagct agacatattg aagtacaaat acttggagat agctttggta
     9001 agatagttca cctaggagaa cgtgattgtt ctctgcagcg taacaatcag aaggtattgg
     9061 aagaaagtcc gtctgtggca ataggtaaca ctctgcgtca acaaattggt gaagcagctg
     9121 ttcgtgcagc tgaggctgta tcttatgaaa atgcaggaac aattgagttt ttattagatg
     9181 agaatagcgg acagttctat ttcatggaaa tgaatactcg tgttcaagta gaacaccctg
     9241 ttacagaatt tgtaacgggt gttgacatcg ttaaagagca gattcgtatc gctgctggaa
     9301 ttcctttatc agtttctcaa aatgatatta agttaactgg tcatgcgatt gaatgtcgta
     9361 ttaacgcaga gaatccacaa tttaattttg cgccatgtcc aggtaccatt aatgggttac
     9421 atctacctgc tggcggtatg ggcttacgtg tcgattcagc ggtttacaca ggctacacta
     9481 ttcctcctta ttatgatagt atgattgcga aagtaattgt tcacggagaa aatcgttttg
     9541 atgcacttat gaaaatgcaa cgtgccttat atgagcttga aattgatggt attataacaa
     9601 atacagaatt tcaaatggat ctcatttctg ataaaaaagt cttggctgga gattatgata
     9661 cttcattcct gatggaagac tttttaccaa ggtattatgg taacaatcaa taggagggaa
     9721 gatggcttta tttagtaaaa aggataaata tatccgtatt agtccaaaca aagcattagg
     9781 gtcttctgat aaacgtagtc ttcctgaggt tcctgatgaa ctctttgcta agtgtccttc
     9841 ttgtaaacat atgatttatc agaaagattt aggattagca aagatttgtc cagcttgttc
     9901 ttacaatttt agaatttcag cgcaagaacg tctgttgcta acagttgacg aagatagttt
     9961 tgaggaatta tttacaggga ttgaaactaa ggatcctttg aattttccaa actaccgtga
    10021 aaaactggct gctacacgtc aaaagactaa cttagatgaa gcggtggtga caggtttagc
    10081 taaaataaaa ggacaaacta cggccttagc tattatggat tctcatttta taatggcgtc
    10141 tatgggaacg gttgtaggcg aaaagttgac aagattattt gagctagcaa ctgaaaagaa
    10201 attacctatt gttattttta cagcgtcagg gggcgccaga atgcaagaag gtattatgag
    10261 tttgatgcaa atggcaaaag tatcggcagc agttaaacgc cattctaacc agggattgtt
    10321 ttatttaaca attttaacag acccaacaac cggcggcgtt actgcgagtt ttgctatgga
    10381 aggtgatatt attctggctg agccgcaagc gttggttggt tttgctggtc gtcgtgttat
    10441 cgaaacaacg gtgagagaag atttacctga aggctttcaa aaggcagagt ttttgttaga
    10501 acatggtttt attgatgcta ttataaatcg aacagaatta cgcgactgca ttgctcaact
    10561 aattgctttc catggaaatg aggttggtca tgaataatga tgtgacaaga attttaaaag
    10621 atgcgcgtga tcaagggagg ctaactgctc ttgactacgc tgaattgatt tttgataatt
    10681 ttatggaact tcatggtgat cgccagttcg cagatgacaa atcaattata ggaggtttgg
    10741 ggtacttggc agggcgtcct gtgacgattg tcggtattca aaaaggtaag aaccttcaag
    10801 ataatttaga ccgtcatttt ggtcaaccac atcctgaagg ttatcgtaag gctttacgtt
    10861 taatgaaaca agctgagaag tttggaagac ctgttataac cttcatcaat actgctggag
    10921 catatcctgg ggtaggtgct gaggagcgag gtcagggtga agcaattgct agaaacctct
    10981 tagaaatgag tgatttaaaa gtccctatta tagctattat tattggtgaa gggggttctg
    11041 gaggagcatt agctttagct gttgccgata aggtttggat gttggaacat actgtttatt
    11101 ctattttaag tcctgagggc tttgcctcta ttttatggaa agatggtaca agaacaacag
    11161 aagcagcaca attaatgaag atgactgctg gagaacttta ccatatggaa gttgtagaca
    11221 aagtaatccc tgaacatggt tatttttcaa gtgagattgt cgatatgatt aagacaagtt
    11281 taatttcaga gttagaaatg ctctcacaat tatctcttga ggatttattg gagcaacgtt
    11341 accaacgttt tagaaaatat taatagaaaa aggctgagtt atttactcag cctatattta
    11401 gtttttcaag gtattatcat agttatagtt tcatattttg ctataattga taaaaaggag
    11461 atatgtcaat gacactagca ttagccaatt tttagattgt agtctaatcc aaaattttca
    11521 gaaagagtca tttcgaacac tttattctaa gtaccattat ttaggctctc cttaacttga
    11581 gactctatct gttattgaag agaaattttc tagaaaaaat agctattgtt agattttaga
    11641 aggctatgaa aagattggtt tttaaagagt tctattaagt gatgaagaaa gtaaaacttg
    11701 gttagttata attgcgatta gactttgttt ctagcaacga gacttcgcta tacatacttt
    11761 aaaagaactt gaaagtatgt atagatatat aatcgtgaaa acttaactat tgtttacatg
    11821 gagaaaaaga atgtatcaag aattaaatga ttatttagaa ttccaatcaa tgaagtatat
    11881 tatgcctgag aaggcaggcc ctttaaagga cgatatgcaa acttttaagg caaagggcca
    11941 acttgctagg aatagcttta cagagttatc aagagcctta gaacaacgta tggatggctt
    12001 taaaatgcaa agagtgagta attgggctaa tcaagctcaa gtagggcgtc cacatttttg
    12061 ggtttattat cgaaaggata cagaccaatt agatgatgtg gctgttgcgc taagggttta
    12121 tggtgtaaaa gatagttttg gggttagtct tgaggtaagc tttgtagaga ggcaaaaatc
    12181 tgataagaca ctcgagaagc aggctagagt gttatcgatt ccgattgcct ctccattgta
    12241 ttttatggtc caaaggcaag gtgaaactca tcgagaggaa ggcaatgagg aaaatcgtca
    12301 acgtttaatg caagaaatta agtcagggaa ggtaaggaaa gtattggtaa aatatgatgt
    12361 tcttttgact gaaaaccaga gcttagaaaa tattctgcaa cggttgttag aaggatttga
    12421 gaaagtgcta ccttactacc aagtttgtca aactattagc taagttcatg gaaaaaatga
    12481 gttggtatac caactcattt tagagtgcct aatttggttt aataatatcg atattaccca
    12541 tatatggacg aagaacttct gggatagtca cagaaccatc ttcattttga taattttcga
    12601 ggattgctgc tacagtacgt ccaactgcaa gtcctgaacc atttaaagta tgaagcaacc
    12661 taactttgcc atcaacttcg tcacggtaac gaatttgagc acgacgtgct tggaaatctt
    12721 cagtattaga acatgaagaa atttctcgat aagtattttg agcgggaatc catacctcta
    12781 agtcatacgt tttagctgct gagaatccca tatctcctgt acacaaggta atgacacggt
    12841 atgggagatt aagtttttga agaatatttt cagcatttgc tgtcattttt tctaattctt
    12901 ggtacgattc ctcaggcttg gcaaatttca ccatttctac tttatggaat tgatgaaggc
    12961 gaattaatcc acgtgtgtca cgtcctgcag agcctgcttc tgaacggaat gatggactca
    13021 tggcagtaaa gtatataggt aattctttcc catcgataat ttcatcacga taatagtttg
    13081 ttaaaggaac ttcagctgtt gggataagta cgaatgggct atctgctaat tcgaaagtat
    13141 cttccttgaa ctttggatat tgtcccgtac cgaacattga atcgtggtta accatataag
    13201 gagggatcac ttcagtgtac ccttccttag cgtgttcatc cagcatgaag ctgtagatag
    13261 cacgctctaa acgagcacct agacctttgt agaagaggaa gcgagaacca gtaacttttg
    13321 caccacgttc ccaatcgaga attccaaggc tttcccctaa atcccagtga gctttggcct
    13381 caaaatcaaa ttcacgagga gttccccagc gacgaacttc aacattttca tcctcgtctg
    13441 ctccaagcgg cacatcatca gctggtgtat taggaagtac ggtcaccatt gattgtaaat
    13501 tagcatccac atctgccaat tccgcatcaa ttgctttgat atcagcggaa agtgtttgca
    13561 tagctgtgat ttgctcgtct gcattctctt tatttcgttt tgcttgagca atggcagcag
    13621 aagcaacatt acgttgcgct ttcgcctctt cagatttgat aagtaattca cggcgtttta
    13681 tatcaagttc tttaagggtt gttaatgttt cttggtcaac accacgcgtg gccaattttt
    13741 tagcgacagc atcaaaatct gtacgaatac gttttaagtc taacatatag gaatctcctt
    13801 tatactaaaa aaaacaccct gtcatgctgg agcgcaaagg cgcggttcca tccagcttca
    13861 cagaagtgca cttgattgat tgattaaatt atttcccata aatggaacac ggtagaattt
    13921 cataaaacaa tcttatctgc tcgcaacgac cgcagactct ctgaaaagaa ctattatact
    13981 acttatccgt taaggttatt atatccaaaa ataagtaaaa tgtaaacctt aactttataa
    14041 gcctgctctt tgttttagtg aaatagctat gctaaaatat gattatggaa aagtatacaa
    14101 gtattaaaca atcaggtcgt cattacggct tagacctttt aaggattatt tccatgttca
    14161 tgattgttat tacgcatgtt cttggaaaag gtggtctgag atcttcggtt gagggacacg
    14221 cagactccta ttttatagta acgtggatta tccaagtctt agtttatggg gccgttaact
    14281 gttacgcact aataagtggt tatgtgggaa ttaattcgcg ttatcgttac tcaaaattac
    14341 tgtctatctg gacacaggtt tttttctaca cctttacaat aacagcactt tttgcaatca
    14401 caggtcatga agtgaccttg cttaattgga gagatgcttt tttccctatt gtcagtgggc
    14461 agtattggta cattactgct tactttggtc tattggtttt tatgcctgtt attaataatg
    14521 gattaaatgc cttgactgat aaacaattaa aacaattggt tttgttaatg tttattattt
    14581 tttcaatttt accagctgtt ttaaacaatc gtgttccaga attttcattg agcaagggtt
    14641 ttgaaatgac ttggctactg attttatata ttattggtgc ctatttgaaa cgtattgatt
    14701 taaatatatt taaaacatca tacttactta tagtttatct attatcattg gttgctactt
    14761 atgtcatgaa gtttagtatt ggtgatattt ggtattggta tgtttcgcct actttaactt
    14821 taggggcggt aagtttgttt atattatttg ctagagcaag cattaagccc tcgggattct
    14881 taaagaaaat aattgttgta ttagcccctt caaccttggg agtttatctc tgtcatctcc
    14941 acccactaat agtaaaatat tttgtaagag attttgcaga gacgtttgtt tatgagtcca
    15001 tttatcttta cccattttta attttaggag cagggatttt aatttatttg ttggctttta
    15061 ttgtagaacg tattagatta cgaatattta aagcaatcca tttttctaac tttttgagat
    15121 ggtttgatga caagttccca tattaatatt agagttaaaa aataagatag actatagtct
    15181 atcttatttt ttaaacttga atatttttaa aatggtctgg atgagggtag gaagcttaac
    15241 aactttatcg tctccaatat gtcgtctcgc atgctttaat atcgttccag agtctttaga
    15301 ggcaaataaa aattttcctt ggtctgtaaa gacctcaaag tgacgactaa ttttttttcg
    15361 actaacatta gctccaattt gattgattga tgtccatgga atttgaatat agtcgttgac
    15421 atttttatcg ttatagaatt ctaatgcctg gtcaccaact aaaaattttc caactttatt
    15481 ccccatacct aaatacgaaa ctcctgttgt ttgaaattca acagttttgt ttaatgattg
    15541 tgccatgact aatccctttt taatttatag tagtttgatt atatcataat agtgggactc
    15601 taagtggatt tttggtaata aaatataaga gatggaatac tattgtaacc atctcttagt
    15661 tttattacat cacacctgca aggcgagcta gaataccaac gataaacaaa ccgatgataa
    15721 ttgtaattgg tgatactttc ttcttaagta accacataca gaagaatgta agaagtaatc
    15781 cagctaaacc aggaataagt gagtcaagtt gtccttgaag tgtttgtggt tgcattttat
    15841 caaggcttaa gccactctga acttgtccta ggatatcatg caattgttgt ccactaacat
    15901 ggtctttagg gaaattgatg tatgcccctt ctgacaatgt ctttttagga aggtctacag
    15961 taaagttaat tgctacccaa cgcttaacaa ggacagcaag aatgaacata ccaaggattg
    16021 aagcaccttt agtaatatct tggaggatac cgccagacat atctttagta atttctttac
    16081 ctgatttgta acctaactct tgtgtatacc ataagaatga catacgaatg aggttccaac
    16141 caacaaagaa gatgattggt ccgaggatat taccagcaga ggcaagtgaa gcaccaagag
    16201 cacctaagat aggacgtaca gtaaaccaga atactggatc accaatacca gcaagaggtc
    16261 ccatcatacc gattttaacc ccttgaattg ctttgtcttc gacaggtgta ccgcttgctt
    16321 tttcttcctc aagagcaagg gttacaccga taattggagc agcaacgtat ggatgtgtgt
    16381 taaagaattc catatggcgc tcaagcgcag cagcacgatc ttctttagta gtgtaaagtt
    16441 ttttcaatgc tgggataaga gcgtaggccc accccatatt ttgcatacgc tcatagttcc
    16501 aagaaccttg aaggaattgt gaacgccacc aaactttttg acgatctgat tttgataatt
    16561 taatttgttc agtcattgtt tagctccttt ctagtagtcc tctaagatat cgccgatagg
    16621 atcgttagaa cctgcgttag aagtaccttt gcttccaccc atttttgata aactgatgta
    16681 gatgagggcg atagcaacac caatagtacc cattgcaata agagttaact ggttcaaagc
    16741 tgctagagca aatccaagag caaagaaagg ccaaacttcg cgagtagcca tcatattgat
    16801 aaccatagcg taaccaacgg caacaaccat ggcaccacca acttgcatac caccatttaa
    16861 ccaatcaggc attgcttcaa gaatactttg tactgaagaa gatggaatag ctaaaagaag
    16921 cgcagctggg aatgcaatac gtaatccttg gagaaggagg gcaataaagt gaaaacgttc
    16981 aattgctgcg aagtttcctt cagaagcagc tttgtcacca gcgtgaacaa gtgcagttga
    17041 gattgtacga acaatcattg taaggaaaag tccagcaata gctagaggta cagcagtaga
    17101 gaaggcaaag ttgatacctt tttgagtgaa gtcgccactt ttaaccaaaa taatagcagc
    17161 agcaacagag gcaagtgcag cgtctggtgc tacggcagct ccgatatttg cccaagcaag
    17221 agcaatcatt tggagactac caccaagaat aatacccgct tcgaggtgac cagttacaag
    17281 accgattaaa gtacaagcaa caagaggttg atggatttgg aattggtcga ggataccttc
    17341 aagaccagca aagaaggcaa cgataacgac caaaatcatt gagataattg aaatatccgc
    17401 cattttgata tcctttcgtt ttcagagata ataagttatt ttacgtttgc tttgttgatt
    17461 aaatcaaaca aattcttttt agcgtcattt ggtactttac gtacatcaaa tgatacacct
    17521 aagtcacgta atttttcaaa ggcagcaaca tcgtctttat ccattgaaag aacgttgttt
    17581 accattgttt taccagttga gtgagccatt gaaccaacgt tcaattcagg gatttccaca
    17641 ccaccttcaa ctgcacggag tgcatcttgt acagtttcaa ataagatcaa ggcacgtgtg
    17701 tttccgaaac gtgggtcttt agctacttca attaatttac taattgggac aacgtttgct
    17761 ttgactcctc caggagcagc ttgcttaatc aattgtttac gaagttcatc tttagaaact
    17821 tcatcagaag caacgataat acggtttgct tttgatgctg gagtccatgc agttgcaact
    17881 tgaccatgta aaagacgtgt gtcaatacga gcaagattaa ttttaagctt gccatctcca
    17941 ataacagttc cttcagggat agatccactt tgtttaactt ccgcaggaac atctgcagga
    18001 acacctgcta caggagtagc ttctacaaca ggattaagtt cttctggaag ggctttgata
    18061 ccttccttag actctttaat aatatttgcg gcaacttgtt caacacctgc attagcgtcc
    18121 atcatacgct cagtataggc ttggataagc attggcaaat tgagaccagt gatgatagcc
    18181 attttacgtt caggattttc tcccataaca cggcttgctt ggttaaatgg tgacccactc
    18241 caaaggtcag ctaacacaag cacctcatca tcagcatcaa attgtgcgat agcattgttg
    18301 aagtgtccat acaaatcatc tggtccttca tttggcatga aagttacaac ttgaactttt
    18361 tcttgttcac caaagatcat tgaacctgat tgatgaatac cttcagcaaa tttaccatgg
    18421 ctggcaataa taataccgat acccattctt gttcttcctc cttaaaaaat tttttcgttg
    18481 acataaagaa gaataatctc cttccaatcg taaaattggc cattcttttt catttcaacg
    18541 aaattataga acagatatat tttaaaacgt tttcagaaaa aaatcaatac tatttctaat
    18601 aaaa
//
LOCUS       DATROY010000023        18231 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.023, whole genome shotgun sequence.
ACCESSION   DATROY010000023 DATROY010000000
VERSION     DATROY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18231
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            146..571
                     /gene="rplK"
                     /locus_tag="VBD11_000699"
     CDS             146..571
                     /gene="rplK"
                     /locus_tag="VBD11_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6953670.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            675..1364
                     /gene="rplA"
                     /locus_tag="VBD11_000700"
     CDS             675..1364
                     /gene="rplA"
                     /locus_tag="VBD11_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6953671.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1753..3369
                     /gene="nikA"
                     /locus_tag="VBD11_000701"
     CDS             1753..3369
                     /gene="nikA"
                     /locus_tag="VBD11_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO6953672.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            3356..4300
                     /locus_tag="VBD11_000702"
     CDS             3356..4300
                     /locus_tag="VBD11_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953673.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4300..5106
                     /locus_tag="VBD11_000703"
     CDS             4300..5106
                     /locus_tag="VBD11_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953674.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5094..5882
                     /locus_tag="VBD11_000704"
     CDS             5094..5882
                     /locus_tag="VBD11_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953675.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5869..6549
                     /locus_tag="VBD11_000705"
     CDS             5869..6549
                     /locus_tag="VBD11_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953676.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            6670..7398
                     /locus_tag="VBD11_000706"
     CDS             6670..7398
                     /locus_tag="VBD11_000706"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6953677.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            7414..7971
                     /gene="frr"
                     /locus_tag="VBD11_000707"
     CDS             7414..7971
                     /gene="frr"
                     /locus_tag="VBD11_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6953678.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            8089..8943
                     /gene="cvfB"
                     /locus_tag="VBD11_000708"
     CDS             8089..8943
                     /gene="cvfB"
                     /locus_tag="VBD11_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6953679.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            9081..9590
                     /gene="msrA"
                     /locus_tag="VBD11_000709"
     CDS             9081..9590
                     /gene="msrA"
                     /locus_tag="VBD11_000709"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6953680.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            9587..9802
                     /locus_tag="VBD11_000710"
     CDS             9587..9802
                     /locus_tag="VBD11_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6953681.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            9959..11731
                     /locus_tag="VBD11_000711"
     CDS             9959..11731
                     /locus_tag="VBD11_000711"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6953682.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            11855..12841
                     /locus_tag="VBD11_000712"
     CDS             11855..12841
                     /locus_tag="VBD11_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6953683.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(12932..13735)
                     /locus_tag="VBD11_000713"
     CDS             complement(12932..13735)
                     /locus_tag="VBD11_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6953684.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            13927..14403
                     /locus_tag="VBD11_000714"
     CDS             13927..14403
                     /locus_tag="VBD11_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6953685.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            14725..15531
                     /locus_tag="VBD11_000715"
     CDS             14725..15531
                     /locus_tag="VBD11_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6953686.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DLDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            15926..16423
                     /gene="ybeY"
                     /locus_tag="VBD11_000716"
     CDS             15926..16423
                     /gene="ybeY"
                     /locus_tag="VBD11_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6953687.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            16404..16802
                     /locus_tag="VBD11_000717"
     CDS             16404..16802
                     /locus_tag="VBD11_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6953688.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            16844..17743
                     /gene="era"
                     /locus_tag="VBD11_000718"
     CDS             16844..17743
                     /gene="era"
                     /locus_tag="VBD11_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6953689.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            17980..>18231
                     /locus_tag="VBD11_000719"
     CDS             17980..>18231
                     /locus_tag="VBD11_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953690.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFI"
BASE COUNT         6071 a         2684 c         3616 g         5860 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttca
      181 aattcctgct ggtaaagcaa ctccagctcc accagttgga ccagcacttg gtcaagcagg
      241 aatcaacatc atgggattca ctaaagagtt taacgctcgt acagctgatc aagctggtat
      301 gattattcca gttgttatct cagtatatga agacaaatca tttgatttca tcactaaaac
      361 accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtgaacc
      421 taacaaaact aaagttgcaa caattactcg tgcgcaagta caagaaattg ctgaaactaa
      481 aatgccagat ttaaacgctg caaaccttga gtctgcaatg cgtatgatcg aaggtactgc
      541 tcgttctatg ggattcactg ttactgacta atcagaaaca cccacataac ccgcaagact
      601 tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca aatcgattga ccacattaca
      661 aggagaattt taaaatggct aaaaaaagta aaaacttacg tgctgctctt gagaaaattg
      721 acagcactaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
      781 ctaaatttga tgcaactgtt gaagtttctt ataaccttaa cattgacgtt aaaaaagctg
      841 accaacaaat ccgtggtgct atggttttac cagcaggtac tggtaaaact tcacgtgttc
      901 ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc agcaggtgca gactttgtcg
      961 gtgaagatga cttagttgct aaaatccaag gtggatggct tgactttgat gttgttatcg
     1021 caacaccaga tatgatggca cttgttggac gtcttggacg tgtccttgga cctcgtaact
     1081 tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt tgctaaagca gttgaagagt
     1141 ctaaaggtgg taaaattact taccgtgctg ataaagcagg taacgtccaa gcccttattg
     1201 gtaaagtttc atttgatgat gctaaattag ttgataactt caaagcattt aatgatgtta
     1261 ttgttaaagc taaaccagct actgcaaaag gaacttacat cacaaacctt tcaattacaa
     1321 caacacaagg tgttggtatc aaagttgatc ctaactcact ttaatcaata aaaaactagg
     1381 aattccttcc tagttttatt tattctatat acttaactag ttaagtatat attttaataa
     1441 tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt ttatctgtat taaccttctt
     1501 aaagactaat gaatgtgcga tgtgttttaa gtaacagatg agaataagga aaaatgaaat
     1561 tggcgatatg gtatgtgact attatgatta ttctttagca ataatctatt aaaatgagtt
     1621 cgcagggaat aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag
     1681 acattactaa aatgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga
     1741 aagttggtaa aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact
     1801 ctgactgcat gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg
     1861 cttgcttggg gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt
     1921 gttatccagg atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa
     1981 ccagctttag cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg
     2041 agaaatgcta aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt
     2101 gatagtattt tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa
     2161 ttggaaaatt atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat
     2221 agtgctactt tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct
     2281 ttccctaaag gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa
     2341 tgggttgtga aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac
     2401 tggggaaaga aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg
     2461 cgtgctttag cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt
     2521 ttagacactt ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct
     2581 atgtcaacaa gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt
     2641 cgtcaggcaa tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga
     2701 acagaaaagc cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta
     2761 aatccttata gctataatgt tgataaagca aatcagctat tggatcaagc tggttggaag
     2821 atgggaaaag ataaggttcg tgaaaaagat ggtaaagctc taaccttacg tcttccttat
     2881 atcgccacta aggcaactga taaagacctg gtaacttatt tccaaggaga atggcgtaaa
     2941 atcggtatta atgtttctct tattgctatg gaagaagatg attattgggc taatgctaaa
     3001 aagggtaatt ttgatatgat gttaacctat tcttggggag caccatggga ccctcatgct
     3061 tggatgtcgg cattaactgc caaggcagat catggacatc cagaaaatat agctttagaa
     3121 aatctagcaa ctaaaactga aatggataga cttattaagt cagctctagt agatcctaag
     3181 gaagaaaatg ttgatagaga ctataagaag gttcttgaat tattgcatga tgaggctgta
     3241 tatatcccat taacttatca atcggtgatt tctgtttata ggaaaggtga ttttaaaacc
     3301 atgcgttttg cgccagaaga aaattcattc ccattacgct atatagaaaa aaacaatgta
     3361 tctaagtagt tcgatcatca aaaaaatatt gtcagctttt ttggcattgt ttttcatatc
     3421 acttctaaca tttattctta tcaaactatc aacagttaat ccagcagaaa attaccttcg
     3481 tttatcaaaa ataagtgtta gtccagaagc gctaaaagaa gcagagcact atttaggttt
     3541 agataagcca ttatggaagc agtactggct gtggttccaa aaagcattaa caggagattt
     3601 tggatattct tatgtgctca ggttgccagt attagatttg gttttacaac ggtttttggc
     3661 tactttattt ttaggcacga gtgccttctt gctaattgtt accatttcta ccccgttagg
     3721 tgtgtgggct ggcttacatg aatctgctcg gagtgatcac ttgattcgat ttttgagttt
     3781 ttctagtgtg tctatgccaa atttttgggt tgcttacttg ttgatgctgc tattttcggc
     3841 aaaattaaat cttctgcctg tttcaggagg taacgattta caaagtttaa ttttgccaag
     3901 tattacctta agtttttcta ccgtaggtca atacatagcc cttattcgaa aagctattag
     3961 tcaagaaaat agaagtttaa atgtggaaaa tgcccgatta agaggagtaa aagaacgtta
     4021 tattgtcaca catcacctcc ttagaaatgc cttacctgcg ataatgacag cacttagctt
     4081 gacttgggtt tacttattaa caggatcaat tattgttgaa gaaatttttt catggaatgg
     4141 aataggacgt ttatttgtga ccagtctaag aacttcagat cttccagtaa tacaagcttg
     4201 tatgctaatt tttggaacct tatttttagc taataatttt atgacacagt gttttatgaa
     4261 ttgggttgat cctcggttac gaaagtcaag ggagaaataa tatcaaaaag aatttatttt
     4321 tcgagtagct tactggtatt gttagtgatt agtgccattt ttgcccctat tctatctagc
     4381 tttgatccac aatacgtaga tttatcacaa aaattattgg ctcccaataa tgttcatttg
     4441 ttagggactg accaattggg tagagatgta ttatctcgct tgctttatgg tgctagatat
     4501 tcactgtttt tagctattat tattagctta ttggaattaa cgattggtat gtttgtgggt
     4561 cttattgttg gttggtatca aggaaagtta gaaaacctct ttttatggat agctaatatt
     4621 attttagctt ttccgagctt tttgctctct cttgcaaccg tggggatctt aggtcatggt
     4681 ctagggaatt taatttttgc aattgtcttt gtggaatggg tttactatgc taaattaatg
     4741 accaatttag ttaagagtgc aaaaaaagag ccttatgtga taaatgcgca aattatggga
     4801 ctttcagtct ggcatatttt aagaaaacat atttttcctt ttgtgtatca gccaatcctc
     4861 gttatggtcc ttatgaatat aggaaatatt attttaatga tttctggctt ttctttctta
     4921 gggattggtg tgcaacctaa tgtcacagaa tggggaatga tgttgcacga tgctagagga
     4981 tattttagga cagctacttg gatgatgtta tctcctggaa ttgcgatttt tttaacagta
     5041 ttttccttta atactttagg cgatgccata gataaaaaag attggaaacg acaatggaac
     5101 agttagaaat aagaaaattg tcattacaga taggagaggt ccctgtacta agagatttta
     5161 gttgtaaaat agatatggga gaatcgctaa ctattattgg tgagagtggt tcagggaaaa
     5221 ccctcttggc aaaattattg gttggtcaca ttccacaagg tatgacagtt agaggaaaca
     5281 tattttttaa gggagttgat ttaggtaaac taactgtaaa gcagtggcag aaattaaggg
     5341 gacgagatat tgcctattta gtgcaaaatc ccatgtctat gtttaatcct tttcaaaaaa
     5401 ttgaagcgca tattttggaa acaatcctta gtcatgagaa atgttcaaag agagtagcct
     5461 tatctaaagc gttagaatgg atgaaacgtt taaatttaga tgatgcgata tccctgttaa
     5521 aaaaataccc ttttgagctt agtggaggaa tgctacaaag aattatgtta gccactatat
     5581 tatctcttga tcctcaggta atcatattag atgagccgac ctcagcggtt gattgtcata
     5641 attgttctac tatatcagct attttgcagg aattgcaaaa taatggaaaa accttgatta
     5701 ctgtaacgca tgattatcag ttagctagag acctgggggg acaattatta gttataagtg
     5761 agggagaagt tgttgaacag ggacaaaccc aagctatttt aagcaatcct caacataact
     5821 atacgaaagc actgacagtg caaatggagt atgaaggaga tattcttaat gttggtttgt
     5881 aatcatgtcg gaaaaacatt tggacgacaa gaggttttaa aggactgtca ttttcatctt
     5941 aaaagaggag aaataattgg tataatgggt aaaagtggca gtggtaaaag tagccttgcc
     6001 cgactcatta taggactaga tagtcctact tgtggttcga tatattttca aggaaaaatt
     6061 tacacaccta aagatggtaa ggcgcagatt atccttgttt ttcaagatgc gcttagttcg
     6121 gtcaatccat attttagtat tgaggaaatt ttgaatgaag ctttttatgg aaaaaaaaca
     6181 acttttgaat tatgtcaaat attagaagcg gtgggtttag atggaactta tctaaaatat
     6241 aaagctagac aacttagtgg tggtcagcta cagagagttt gtatcgctag agctttactc
     6301 ttaaagccta aaattattat ttttgatgaa tccttaagtg gattagaccc agtaactcaa
     6361 attaaaatgc tacgtttatt acaaaaaata aaacgccgtt atgaattaag ttttataatg
     6421 atttctcatg accctaaaat ttgtcaagct atctgtaacc gggtctttct gataaaaaat
     6481 ggttatttag tagaagataa tgaattcttg aagagagcgt gttctactaa ctgtttgacc
     6541 aatctctgaa ttattatgtt tttcttattt taggagttga taaagaaatc aacttactag
     6601 tgtcattgct ctattaaaat tttgcctttt atggtaaaat aataaagaca aaataaggag
     6661 aacatagtag tggaacctaa atatcaacgt atattaatta agttatctgg tgaagcattg
     6721 gcgggagata agggtgtcgg tattgacatt cctaccgttc aatctattgc aaaagaaatt
     6781 gccgaagtac acaattcagg tgttcaaata gcacttgtca ttggtggagg taatctttgg
     6841 cgtggagaac ctgcagcaga agcagggatg gatcgtgttc aagctgatta tacagggatg
     6901 ttagggacag taatgaatgc tcttgtaatg gctgatagcc ttcaacaata tggggtagat
     6961 actagagttc aaacagctat tccaatgcaa actgttgctg agccttacgt acgtggtcgt
     7021 gctttacgtc atctagagaa gaatcgtatt gttgtctttg gagcgggtat tggttcgcct
     7081 tatttctcaa ctgatacaac agcagcgctt cgtgctgctg agattgaggc agaagcaatt
     7141 ttaatggcta aaaatggagt agatggtgtt tataatgctg atccaaagaa agatgccaat
     7201 gctgtcaaat ttgatgaatt aactcacgtt gaagtaataa aacgagggtt aaaaatcatg
     7261 gatgcaaccg catcaactat ttcaatggat aatgatattg accttgttgt tttcaatatg
     7321 aatgagactg gtaatattaa gcgtgttgtt cttggagaac aaatcggaac aactgtttca
     7381 aacaaagcat ctgaatagga gtgtataaaa aatatgacta aggaaatcgt tacaaaagct
     7441 caagagcgtt ttgagcaatc acaccaaagt ttatcaagag aatttgcagg gattcgtgca
     7501 ggacgtgcta atgctagctt gcttgatcgt attcaagttg agtattatgg agcgccaacc
     7561 ccacttaacc aattagcttc tattactgta cctgaagctc gtgttctttt aatttcacca
     7621 tttgataaat catcaatcaa agatattgag cgtgctatta acgaatcaga tttaggtatt
     7681 aatcctgcaa atgatggttc agttattcgt cttgttattc cagctttgac agaagaaaca
     7741 cgtcgtgatt tagctaaaga agtgaaaaaa gtcggagaaa atgctaagat tgctatccgt
     7801 aatattcgcc gtgatgctat ggatgaagct aagaagcaag aaaagaataa agaaattaca
     7861 gaagatgact tgaaatctct tgaaaaagat attcaaaagg ctactgatga tgctgtaaaa
     7921 catattgatg agatgactgc taataaagaa aaagaattgt tggaagttta atagtaacgt
     7981 ttgctatctt tattatgatt ggcaagtaat aaaatgatta tggaggtaac tgatagaatc
     8041 aaaaccagat ggatttggtt tgtcggttac ctttttaaaa ggagaaaaat gaatacatta
     8101 ttagcgacag tcataacagg cttagtaact gatgaaaaca aagattttta ctttatccaa
     8161 aaagatggct ttacctttgc attatcaaag tcagaaggag agcatcacat tggtgaaatg
     8221 gttaaaggat tcgcctatac agatatgcaa caaaaagcac gtcttacaac taaagaaaca
     8281 tttgcgactc gagatcatta tggttggggg acagttactg aggtacgtaa agacttggga
     8341 gtatttcttg atactggtct tccagataag caagtggttg tttctttgga tgttcttcct
     8401 gaattgaaag aactttggcc taaaaaaggt gatcgcctct acgtttgctt agatgttgat
     8461 aaaaaagatc gtctctgggc tctccctgca gatcctgaag tgtttcaacg aatggcaacc
     8521 cctgcatata acaatatgca aaatcaaaat tggccagcaa tcgtttatcg cttaaaattg
     8581 tcaggaactt ttgtttatct accagaaaat aatatgctcg gctttattca ccctagtgaa
     8641 cgttacagtg aacctcgttt agggcaagtg ctggatgcac gagttattgg cttcagagaa
     8701 gttgatagaa ccttaaattt atcattaaag cctcgttctt ttgaaatgtt agaaaatgac
     8761 gcacaaatga ttttaacgta tttagaaagt aacggaggat tcatgacttt aaatgataaa
     8821 tcttctccag aggaaattaa agctactttt ggaatttcca aaggtcagtt taaaaaagca
     8881 cttggaggac tcatgaaagc taaaaagatc aaacaagatc agctaggtac agaattatta
     8941 taaacttttg aacttttaaa gttttctcac gaggtcgttt tagttatagc tagaattaat
     9001 ggctaattat agaaaaaagc tgagaggaaa ttttggcttt ttttgttata atgtatctat
     9061 taaaaagaat ggagaacgat atggaacgtg cgatttttgc tggaggttgt ttttggtgta
     9121 tggtacaacc atttgaagaa ttagatggta ttgaatctgt tttatctgga tacactggag
     9181 gacatgttga gaatccaact tataaagaag tttgtagtaa aactactgga catacagaag
     9241 ctgttgaaat tatttttaat cctgaaaaaa tctcttatgc agatttagtt gagttatatt
     9301 gggcacaaac agatccaaca gacgcttttg gtcagtttga agatcgtggg gataattatc
     9361 gtcctgtcat cttttatgag aatgaagagc aacgtcagat tgctcaaaaa tcaaaagata
     9421 agctacaagc ttcgggtaga tttgatagac caattgtaac cagcattgag cctgctgata
     9481 ctttttaccc agcagaagac tatcatcaag cattctatcg gactaaccct gctcgttacg
     9541 ccttgagtag tgccagaaga catgcatttc tagaggagaa ctggcattga gaaaatcatt
     9601 ttacagttgg ttgatgacac aaagaaatcc aaaatcaaat gaaccagtag caatcctagc
     9661 agattacgct tttgatgaaa ccacttttcc aaaacatagt tcggattttg aaacagttag
     9721 tcgctattta gaagatgagg ccagtttttc ctttaattta acagattttg atgatatttg
     9781 ggaagattat ctcaatcatt aacaataata tagagattgt attttcaatc tcttttttag
     9841 gttatagaaa ttggacacat tgtctaagac tgtctgaaaa gcaggttttt taatggcaat
     9901 cgttttcaca aaggagtaga atatagtcat ctaaaatata gccctaggga ggttgtttat
     9961 gagatataca aatggaaatt ttgaagcctt tgcaagacct cgaaaacctg aaggtgtgga
    10021 taaaaaatcc gcttatattg ttggttctgg tttagcagga ttagctgccg ctgtcttttt
    10081 aatacgtgac ggtcaaatgg atggtcaacg tattcatatt tttgaagaac tacctctttc
    10141 tggaggatca cttgacggtg tcaaacgacc tgatatcggt tttgtaacgc gtggtggtcg
    10201 tgaaatggaa aatcacttcg aatgtatgtg ggatatgtac cgttccatcc cctctctcga
    10261 agttccagat gcttcttatc tagatgaatt ttattggctt gacaaggatg atcccaattc
    10321 atctaactgt cgcctcattc ataaacaggg gaatcgctta gaatctgatg gtgattttac
    10381 actcggaaca cattccaaag agttagttaa gctagtcatg gagactgaag agtctttagg
    10441 tgctaagacg attgaagaag ttttttcaaa agaatttttt gaaagtaatt tttggactta
    10501 ttgggctact atgtttgcct ttgagaaatg gcattcagcg attgaaatgc gtcgatatgc
    10561 tatgcgcttt atccatcata ttggtggtct gcctgatttc acttcattaa aatttaataa
    10621 atataatcaa tatgattcta tggtgaaacc aatcatcagt tatttagagt ctcataatgt
    10681 agatgttcaa tttgatagca aggtaactaa tatctccgta gactttaaga acggtcagaa
    10741 gcttgcaaaa gccattcatc tcacagttgg tggtgaagca aaaacaattg atcttactcc
    10801 aaatgatttt gtttttgtaa cgaatggttc tatcactgaa agtacgaact atggtagtca
    10861 tgatactgta gctaaaccta ataccgattt gggaggttct tggaatcttt gggaaaactt
    10921 agctgctcaa tctgatgaat ttggacatcc taaagtcttc tacaaagata ttccaaaaga
    10981 gtcatggttt gtttctgcta ctgcaacaat aaaagatcca gctattgaac cttatataga
    11041 gcgtttaaca catcgtgacc tccatgatgg taaagtaaat accggaggca ttgttacagt
    11101 tacagattct aattggatga tgagttttgc tatccatcgc caaccgcatt tcaaagagca
    11161 aaaggaaaat gaaacaattg tttggattta tggactctat tcaaatgtgg aaggtaatta
    11221 tattaaaaaa ccaattgaag aatgtactgg tcgagaaatc acagaagagt ggttatatca
    11281 cttaggtgtt cctgaaatga agattcatga tttatctgat aaacaatatg taagtacagt
    11341 tcctgtttat atgccttaca ttactagtta tttcatgcct cgagttaaag gtgaccgacc
    11401 agatgttatc ccacaaggat ctgttaacct agccttcatt ggtaattttg cagaatctcc
    11461 atccagagat acagtattta caacggaata ttctattaga actgctatgg aagctgttta
    11521 tactttctta aatattgaac gtggtgttcc agaagtcttt aattcagcat ttgatattcg
    11581 tgtcttgttg caatctttat actatctcaa tgataagaaa tctgttgaag atatggattt
    11641 accaatacct gccttaatgc gtaaagtagg catgaaaaaa attagaggaa cttatttaga
    11701 agaattactt cgagaagctc atcttttata atgtaaaata aaatcgagaa aacttgtttt
    11761 tctcgatttt ttagttttac aacgactaaa atcaccaaag aaaatgaaat tttgttataa
    11821 tgtagtcact tactgtacga aaaggaagaa taatttgcaa gaatactcta tcgaaattac
    11881 tttacaacat ccagatgata tgatgtcact ctttggcagc aatgagcgcc atttaaaatt
    11941 aatcgaagaa aatttagatg ttatcattca tgctagaact gaacgtgttc aggtattagg
    12001 tgactctgaa gaagcagtag aaaccgctcg gttaaccatt gaagcattat tagtgcttgt
    12061 taaccgcggg atgactgtga atacttcaga tgttgtaaca gccttatcca tggcacaaaa
    12121 tggttcaatt gataaattcg ttgcccttta tgaggaagaa attattaagg atagctatgg
    12181 taagcctatc cgtgttaaga ctttagggca aaaaatatac gttgatagcg ttaaaaatca
    12241 tgatgttgtt tttggtattg ggcctgctgg aacgggaaaa acttttttag cagtcactct
    12301 agcagtaacg gcactaaagc gtggtcaagt taaacgaata atcttaacga gaccagcggt
    12361 tgaagctggt gaaagccttg gtttcttgcc tggagatctc aaagagaaag ttgatcctta
    12421 tttgagacca gtttatgatg ccctatatca aattttaggt aaagagcaaa caagtcgctt
    12481 aatggagcgt gaaatcatcg aaattgctcc attggcttac atgcgtggtc gtactttaga
    12541 tgatgcattt gtcatattag atgaagcgca aaacaccact ataatgcaaa tgaaaatgtt
    12601 cctaacacgt cttggcttca attctaaaat gattgtaaat ggcgatgtta gccaaattga
    12661 cttaccaaag aatgttaaat ctggtttgat tgatgctgtt gagaaattac gaaatatcaa
    12721 aaagattgac tttattcatt tatcagcaaa ggatgttgta agacaccctg tcgttgctga
    12781 aataataaat gcttattcag attcagaaag tagtcacaag ctacaatctg ataaggactg
    12841 atgtttgcca ctaggagacg ttgttagaga gtgtgtcaat atgtaaaaaa cgcctaatgc
    12901 tttaggcgtt tttatgatgg tgtacttcaa cttaacctat aaagtgttga aaattttctt
    12961 taagcatgac gtcgagatgc attattatat cgtttaatgt ttctttaaca gtgtgttcgt
    13021 tggagttgtg atgacatagt ttaatgatat tttcttcgaa atgtttagga agcaatgtag
    13081 cattcttctt agcataggaa atcattcttt tttcgccagg atgtgtcaac ttattaagtg
    13141 caaaaataat atcaaagtag gaatctaaga aagcagtagt tctatggtga gtactaacaa
    13201 agtcttggcg tttaagggct tttataattt gtttatcgta agagggaagc ttgccagtta
    13261 ataagttacg attttgaatg ataatttgtt tttgtaactg actgggataa gaaacttcga
    13321 atctctcttt gagtgattgt aattgatttt caggatcata gagtatttgg caattgatga
    13381 ggttatgcca aaaacaagta gtgtatccaa ttcgagaatt gtggtgttca acgacatctt
    13441 ctaagtctga taaaaagtta tctatgttac gatagagaat atcaatatca gttccgtcgt
    13501 ttaaagtacc attatcttca agctcccagt aatgattgtt aagttcaatg taatgacagt
    13561 gcttgttaag gatacgttta cgactagtga tatcaggagt tgctgcacaa taaacataca
    13621 aatcataatc ggaagattgg tcaaaactgt caccagagcg tgagcctccg agtgctatag
    13681 caacaacatt tggaagttta gagaaatctt gccagagttt tgtgaaatgg tccataaatg
    13741 ctctcctaaa aatgtaaaat attgatgtgt ctccattcta cagggctatc tctttttagt
    13801 caataattaa tagtaagtta ataaaagagg taacggttct aatattcaag aagtgtatat
    13861 gagtgatttg aaaaggagat attttatgct acaatacgta catattagtt taaacaaggt
    13921 gatagaatga aacaagatta tatttcctac attaggtcaa aagttggaca tgagactatc
    13981 tttttaacat attctggtgg tattttgaca gatggtaagg gacgtgtctt actccaatta
    14041 agagctgata agaattcttg gggaattatt ggtggatgta tggagttagg tgagtcatca
    14101 gtagatacat tgaaaagaga atttttcgaa gaaactggat tgagagtgga accaatcaga
    14161 ctgttaaatg tttatactaa ctttcaagac tcttatccca atggcgataa agcacaaacc
    14221 gtcggtttta tttatgaagt aagctgtcca aaaccagtta atatagaagg ttttcataat
    14281 gaggaaacat tgcaattgga ctatttttct aaagaagacg tcaaaaatat tacgatcgta
    14341 aatgaacaac accaattaat tttagatgaa tatttttcac aaacattcca aatggggcgt
    14401 taaaaggatg ggaaatacta ggtgtgactt taaagaactt ctaactttta ttgatatgtt
    14461 gttaggcatt cttgtagaaa caaatgttaa gaaaataagg agttctcatt tgtatatttt
    14521 ataattatta aaaagaaaaa cagtagcttg caataattta gtgactgttt ttttagaaag
    14581 cataagaagt acagaaaaag aaaattatgg aatattggat ggtggggata tgctttactt
    14641 acggtattgg aatgatatac ttaaagaaat ccagttttag aaatccaaat ttctaagtaa
    14701 aatagcataa aggagagtag atgaatgaag ataacattac acggcgttgc tgaaacgtta
    14761 ttaatcacct tatatattag ggctaaagat gcgatggcta aacatccgat acttaatgat
    14821 caaaaatcct tagcaattgt tgaacagata gaatatgatt tggataaatt cgataattca
    14881 gaagcttctt tttatgcaac attagctaga attcgcgtta tggatagaga aatcaaaaaa
    14941 tttattagag aaaatccaaa tagtcaaatc ctttcaattg gttgtggact tgatacaagg
    15001 tttgaaagag tcgataatgg acaaattagg tggtataacc ttgatttgcc agaggttatg
    15061 gagataagaa aattattttt tgaagagcat gaaagagtta ctaatatagc aaaatcagcc
    15121 ctagatgaaa cttggacacg ggaggtaaat ccccaaaatg ccccttttct aatcgtgtca
    15181 gaaggtgttt taatgtttct aaaagaagat gacgtagaga cttttcttca tatcctgaca
    15241 aattcattta gccaatttat ggcacaattt gatttgtgtc ataaggaaat gattaataaa
    15301 ggaaagcaac atgatacagt aaagtatatg gatacagaat ttcagtttgg tatcacagat
    15361 ggtcatgaga ttgtggattt agaccctaaa ttaaagcaaa taaatctgat taactttaca
    15421 gatgagatga gcaaatttga gttaggcaca cttcgctctt tacttccaac aattcgtaaa
    15481 tttaataatt gtttaggtgt gtacgaatat aaagcatctg agaaaaagta acaaaaatct
    15541 atttatataa agagacattc taaatgtaaa tttgtcataa tcgttatagc atttgtagct
    15601 ttgtttattt aggaattagt tttacgtaag ttaggcgctc taaagtttgt attttgaagt
    15661 aaatactaat tcataagtca gaattttatt gaaatggatt ataagtgtgt aatagtgatt
    15721 attagctttc tctccctttt ttaatactct tatggtataa tgatgtgaaa gaatagcttg
    15781 aaggaaggaa gagaacgcac ttttattttg agaactagtt ttagataaaa gtgaaaaact
    15841 taggaaagta gatgtgtcga ttatttagaa ttatgcatta cactttatct tttctaaacg
    15901 ttttgggtcg ttcttactat ataagatgta cgttgaaatg attgatgaaa ctggacaagt
    15961 atcagaagat atcaaaaagc aaactcttga tttgttagag tttgcagcac aaaaaacagg
    16021 taaagagaat aaagaaatgg ctgtaacgtt tgtcactaat gaacgtagcc atgaattgaa
    16081 tttagagtat cgagataccg atcgtccgac tgatgttatc agtttagaat ataagcctga
    16141 ggttgatatt tcttttgatg aagaagatct ggctgaaaat cctgaacttg ctgagatgct
    16201 agaagatttt gattcttata ttggtgaact ttttatttct attgataaag ctaaagaaca
    16261 agcagaagag tacggtcact cttatgaacg tgaaatgggt tttttagctg tacatggttt
    16321 tttgcatatt aacggttatg atcattatac acccgaagaa gaaaaagaga tgttcagctt
    16381 acaggaagaa attttaactg cttatggact taaacgacaa taatcaaaaa aaatggaaaa
    16441 atcgcacctt aacatcaagc atggaatttg ctgttacagg gatttttaca gcttttaaag
    16501 aagaaagaaa tatgcgtaag catttggttt ctgccatttt agtaatactt gctggtctta
    16561 cttttcaagt atctatggtg gagtggttat ttcttttatt gagtattttt ttggtaatta
    16621 cttttgaaat tatcaattca gctattgaaa atgtagttga tttggcaagt aattaccatt
    16681 tctcgatgct ggctaaaaat gctaaagata tggcagcagg agcggtttta gttgtttcct
    16741 tatttgccgt attggtggga ttgattattt ttatacccaa gatacttgct ttacttttat
    16801 gatgagaagt attacgaata aattgaatgg agaaagaaaa gaaatgacat ttaagtcagg
    16861 atttgtagca attttaggtc gtccaaatgt tggaaaatca accttcctaa accatgttat
    16921 gggacaaaaa attgctatca tgagtgataa agctcaaaca acacgtaata aaattatggg
    16981 gatttataca acagaaactg agcaaattgt tttcattgat accccaggaa ttcataaacc
    17041 aaagaccgcc cttggtgatt ttatggttga atctgcttat agtactttac gtgaagttga
    17101 aacagttctt ttcatggttc ctgccgatga aaagcgaggc aaaggcgatg atatgattat
    17161 tgaacgtctt aaggctgcca agattcctgt tattttagta atcaacaaaa ttgataaggt
    17221 tcatccagac caacttttgg aacagattga tgacttccgt agtcaaatgg attttaaaga
    17281 agtagtgcca atttctgccc ttcaaggaaa taatgtgcca accctaatca agttattgac
    17341 tgataatctt gaagaaggtt tccaatattt cccagaagat cagattactg atcatccgga
    17401 acgattttta gtatcagaaa tggttcgtga gaaggtactt catttaacgc aacaagaggt
    17461 tcctcactca gtagcggttg ttgtcgaatc catgaaacgt gatgaagaaa ctgataaagt
    17521 gcatattcgt gcaactatca tggttgaacg tgatagtcaa aaaggcatta tcattggaaa
    17581 acaaggtgct atgctcaaga aaataggaaa aatggcgcgc cgtgatattg aacttatgtt
    17641 aggtgataaa gtttacctag agacttgggt aaaagtcaag aaaaactggc gtgataaaaa
    17701 gcttgattta gctgattttg gctataatga aaaagagtat taatgagttt attttgagat
    17761 ccgcaagtta gtggggaaga gttagataag ttcaccgtga gacttgattc gcacagaaaa
    17821 agcaattcac gaggataaaa ccaactagtc ataattttga gattatattc acaaattaat
    17881 taaagggagc caaacatgct tgttggcccc ttcttaaata aaggagagca catgactcaa
    17941 gattatattt gctacctata aagtagagga ggtgaggata tgcataatat attaagattt
    18001 ttaggcattg ttattatttc tgcagtgatt ctatttagta taggtagttt ctacgattta
    18061 tcattaatga aaaatatact acttatctgt tggagttttc tctttgatct attggttttt
    18121 gtcttcaaac aaagacaaac gacagaagta ctcacatggt atcaagttgt taagcaattt
    18181 tggttattta ttaagtgtac tattcttatt cctatcttag ttgcttttat t
//
LOCUS       DATROY010000024        18004 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.024, whole genome shotgun sequence.
ACCESSION   DATROY010000024 DATROY010000000
VERSION     DATROY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18004
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            266..580
                     /locus_tag="VBD11_000720"
     CDS             266..580
                     /locus_tag="VBD11_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6953691.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            596..982
                     /locus_tag="VBD11_000721"
     CDS             596..982
                     /locus_tag="VBD11_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6953692.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1011..2396
                     /locus_tag="VBD11_000722"
     CDS             1011..2396
                     /locus_tag="VBD11_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6953693.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2399..2551
                     /locus_tag="VBD11_000723"
     CDS             2399..2551
                     /locus_tag="VBD11_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6953694.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2574..3779
                     /locus_tag="VBD11_000724"
     CDS             2574..3779
                     /locus_tag="VBD11_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6953695.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3822..4043
                     /locus_tag="VBD11_000725"
     CDS             3822..4043
                     /locus_tag="VBD11_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953696.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4160..4657
                     /locus_tag="VBD11_000726"
     CDS             4160..4657
                     /locus_tag="VBD11_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6953697.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4632..5138
                     /locus_tag="VBD11_000727"
     CDS             4632..5138
                     /locus_tag="VBD11_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6953698.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5122..7569
                     /locus_tag="VBD11_000728"
     CDS             5122..7569
                     /locus_tag="VBD11_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6953699.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7572..9749
                     /locus_tag="VBD11_000729"
     CDS             7572..9749
                     /locus_tag="VBD11_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6953700.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9746..10747
                     /locus_tag="VBD11_000730"
     CDS             9746..10747
                     /locus_tag="VBD11_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6953701.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10744..11679
                     /locus_tag="VBD11_000731"
     CDS             10744..11679
                     /locus_tag="VBD11_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6953702.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11924..12040
                     /locus_tag="VBD11_000732"
     CDS             11924..12040
                     /locus_tag="VBD11_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6953703.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12056..13975
                     /gene="tet(M)"
                     /locus_tag="VBD11_000733"
     CDS             12056..13975
                     /gene="tet(M)"
                     /locus_tag="VBD11_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6953704.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14094..14261
                     /locus_tag="VBD11_000734"
     CDS             14094..14261
                     /locus_tag="VBD11_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6953705.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14321..14674)
                     /locus_tag="VBD11_000735"
     CDS             complement(14321..14674)
                     /locus_tag="VBD11_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6953706.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15179..15601
                     /locus_tag="VBD11_000736"
     CDS             15179..15601
                     /locus_tag="VBD11_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6953707.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            15598..15828
                     /locus_tag="VBD11_000737"
     CDS             15598..15828
                     /locus_tag="VBD11_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6953708.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16289..16492
                     /locus_tag="VBD11_000738"
     CDS             16289..16492
                     /locus_tag="VBD11_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6953709.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            16574..17791
                     /locus_tag="VBD11_000739"
     CDS             16574..17791
                     /locus_tag="VBD11_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6953710.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         5881 a         3236 c         3756 g         5131 t
ORIGIN      
        1 tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt tatccttgaa
       61 aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta gacacttcaa
      121 aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa
      181 aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc attcagccaa
      241 taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca acatggaaaa
      301 aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg
      361 acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa gagcagatga
      421 tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt
      481 gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc gtggttttac
      541 aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag gaaaaatgat
      601 gagattagca aatggcattg tattagataa agacacgact tttggagaat tgaaattctc
      661 tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa
      721 ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag taagtattcc
      781 tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta tcaatcccat
      841 tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt atatcaaggc
      901 agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac aagcaaagaa
      961 agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc atgaaacagc
     1021 gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg atagcgtcac
     1081 tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc cagcagatca
     1141 actggcagga ttttaaccta tcacaagcag ataagattga cattccctat ttaattatca
     1201 gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct
     1261 atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata cttgaaaaca
     1321 agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt gctggtcgta
     1381 caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat ggcttgatac
     1441 agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac ttggaaaaga
     1501 aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat tcctatgtgg
     1561 aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa gtagaagcta
     1621 aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag ctccctcata
     1681 tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca ctgattgaag
     1741 ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct gaccttgcgg
     1801 acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt tcttgcattg
     1861 aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg aagaactata
     1921 agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc tttgatgaat
     1981 acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg aataagctga
     2041 aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct tgtcaacgtc
     2101 cagacgcaaa atatttaggc gacggaatcc gtgatcagtt taatttcaga gtggctttag
     2161 gtcgtatgtc tgaaatgggc tatggcatga tgtttggcag tgacgtacaa aaggatttct
     2221 tcttaaagcg aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc atatcagagt
     2281 tttatactcc ccttgtacca aaaggatatg atttcttgga ggaaattaaa aagttatcca
     2341 acagcagaca gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt
     2401 gctggctggt gtggcaatag ccacgccagc acttaacccc ccgtatctaa caggggggta
     2461 caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac
     2521 aaaatttcag cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg
     2581 gatttttact gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg
     2641 gactatctca aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata
     2701 ttgaaacagg aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg
     2761 aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg attcgctttc
     2821 cgacaacaga cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc
     2881 ttcatgagga ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg
     2941 tcctttgctc ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca
     3001 gacaatttga aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg
     3061 ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga
     3121 ttttgaatat ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc
     3181 gcagttttaa aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg
     3241 gaaacaccct ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg
     3301 actacgagca gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt
     3361 ttgagattcg attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg
     3421 acaatccaga gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag
     3481 acgattccaa acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga
     3541 acaatcgtga acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc
     3601 tgaactggct atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa
     3661 tcaaccagac gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca
     3721 agcagatttt gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac
     3781 tcaaatacaa attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc
     3841 tttataactg gtttctatca aacgctcaat cactggtgct tttagcaatc gttgtgattg
     3901 gcttgtatct tggcttcaag cgtgagttta gcaaactgat tggcttttta attattgcga
     3961 ttattgcggt tggcttagtc ttcaacgctg ctggagtaaa agacatttta ctagagctat
     4021 tcaatcgcat tattggtgct taaataaaac cgttcttttg tggaatataa gtggttttct
     4081 tatgttccgc aaaggaatgg tacaccaaac gaagtgcggt agggattttt gaatctctac
     4141 aaagaaagga cgtgaatata tggacgatat gcaagtctat attgcgaatt taggcaaata
     4201 caatgagggc gaattggtcg gtgcgtggtt tacctttccc attgactttg aggaagtcaa
     4261 agagaaaatc ggcttgaatg atgaatatga ggaatacgcc attcatgact acgagttacc
     4321 ctttacggtt gacgaataca cttccattgg cgaactcaat cgactatggg aaatggtatc
     4381 ggaattaccc gaagaattac aatcggagct atctgctctg ctcactcatt tttcaagcat
     4441 tgaagaacta agcgaacatc aagaggatat tatcattcat tccgattgtg atgatatgta
     4501 tgacgtggca cgctactaca ttgaagaaac gggtgcttta ggcgaagtac cagctagtct
     4561 tcaaaactat attgattatc aagcctatgg tcgggattta gacctttcag gaacgtttat
     4621 ctcaaccaat catgggattt ttgaaatcgt ctattaaatc tgtcggtaca ttactactgg
     4681 cagattttct attttacggg gtggctcaat cagctacccc tattttttat gaaaggattg
     4741 attacatgaa gaaaatacga agctatacca gtatctggtc tgtggaaaag gtactgtatt
     4801 ctatcaatga ttttagactt ccgtttccca taacctttac gcaaatgaca tggtttgtcg
     4861 tgtcactctt tgcagtgatg atacttggca acttgccccc tctttccatg atagagggag
     4921 catttctcaa atactttggg attcctgtgg ctttcacatg gtttatgtct acaaaaactt
     4981 ttgatggtaa aaagccttat ggatttttga agtctgtcat tgcttatgca ctgcgaccaa
     5041 agctgaccta tgcaggaaaa aaagtaacgc ttggcagaaa ccagccacaa gaagccatta
     5101 cagcagttag gagtgaattt tatggcatat ccaattaaat acattgaaaa caatctcgtc
     5161 tggaataaag acggggaatg ttatgcttac tatgagcttg ttccttacaa ttactcattt
     5221 ctaagtccag aacagaaaat acaagtgcat gattctttca gacagcttat cgcacaaaat
     5281 cgtgatggca aaattcatgc tttacaaatc agtacagaat ccagcatacg ttctgcacaa
     5341 gagcgttcca aaaatgaagt cactggcaag ctcaaagcgg ttgcctatga caaaatcgac
     5401 caacagacag acgctttaat atccatgatt ggcgaaaatc aagtgaacta ccgtttcttt
     5461 atcggcttta agttgcttct caacgatcag gagttttcta tgaaaagtct taccgttgaa
     5521 gcaaaaaatg ctttgtctga ttttgtctat gatgtgaacc ataagctgat gggcgatttt
     5581 gttagtatga gtaatgatga aatcctgcgt tttcagaaga tggaaaagct cttagaaaat
     5641 aaaatctctc gtcgtttcaa aatccgcagg ttagataagg acgacttcgg ctatctgatt
     5701 gaacaccttt acggacagac aggcactgcc tatgaagagt atgagtacca tctatcaaag
     5761 aaaaagctgg ataatgaaac gctgattaaa tactatgact tgattaagcc tactcgctgt
     5821 ttggtggaag aaaaacagcg atatttgaaa atccagcagg aagatgaaac cgtctatgta
     5881 gcttacttta ccattaacag cattgtcgga gaactggact tcccgtcctc tgaaatcttc
     5941 tactaccagc aacagcaatt tacattcccg attgatacgt caatgaatgt ggaaattgta
     6001 gcgaatcgta aagccctatc tactgtccgc aataaaaaga aagaactgaa agacttggat
     6061 aaccacgctt ggcaaagtga taatgaaacc agctccaatg tggcggaagc tctggaaagt
     6121 gtgaatgagc tggaaaccaa tttagaccaa agcaaggaat ctatgtacaa gctgtcttat
     6181 gtggtaaggg tatcagcaaa tgatcttgac gaactcaaac gtcgttgtaa tgaagtgaaa
     6241 gatttttatg acgatttaag cgtaaaactg gtacgaccat ttggggatat gctcggctta
     6301 catgaagaat ttttacctgc cagcaagcgt tatatgaatg attatattca atacgtgacc
     6361 tctgatttcc tcgctggttt aggttttggt gctactcaaa tgctggggga aaatgagggg
     6421 atttatgttg gctacagctt agatactgga cgcaatgtct atctgaaacc tgctcttgcc
     6481 agtcaagggg ttaagggttc agtaaccaat gcgttagcgt cggcttttgt tggttcgctg
     6541 ggtggtggta aatcctttgc gaataacctt atcgtctatt atgcggtgct ttatggggca
     6601 caagcagtga ttgtagaccc aaaagcagaa cgtggcagat ggaaagaaac cttgccagag
     6661 atttcccatg aaatcaatat cgtcactctg acttctgatg agaaaaacaa aggcttactt
     6721 gacccttatg tgattatgaa aaatcccaaa gattctgaat cactggctat tgatattctg
     6781 acattcctta cggggatttc ctctcgtgat ggggaacgct tcccaatcct tagaaaagcc
     6841 attcgtgcag taaccaatag tgaagtacga gggttgatga aagtgattga ggaattacgg
     6901 gttgagaata cgccactaag taccagtata gccgaccata tcgaaagttt tacagactat
     6961 gactttgcac atttattatt cagtaatggt tatgtggagc agtctatcag cttagaaaaa
     7021 caactgaaca ttatacaggt tgcggacttg gtacttcccg acaaggaaac ttcctttgag
     7081 gaatatacca ctatggagct tttatccgtt gctatgctga ttgtcattag tacctttgct
     7141 ttagacttta tccatacaga ccgaagcatt ttcaagattg tagatttaga cgaagcatgg
     7201 agctttttac aggtagcaca aggaaaaaca ctatctatga agctggttcg ggctggtcgt
     7261 gctatgaacg ctggggtata tttcgtgacc caaaatacag acgacctctt agatgaaaaa
     7321 ctgaaaaata acctcggctt aaaatttgca tttcgttcca ctgaccttaa cgagattaaa
     7381 aagaccttag ccttttttgg tgtagaccca gaggacgaaa acaatcagaa gcgattgcgt
     7441 gatttggaaa acgggcaatg ccttatcagt gatttatatg gtcgtgtcgg tgtgatacag
     7501 ttccaccctg tatttgaaga actgctccat gcctttgata ccagaccacc tgtgcgaaaa
     7561 gaggtgtaaa tgtgaaacca tcaatagtaa acagaataaa atcaaactgg acgctgaaac
     7621 gtctaggtaa agtggcaatg acagtggctt tcacacttgt gattgccatt tttcttttag
     7681 ccatgctggg aacggtggtt caagctgcgg gcttggtaga tgatacggtc aatgtggcaa
     7741 atgaatacag ccgataccca cttgaaaact atcaactgga tttttatgtg gataatagct
     7801 ggggctggct tccgtggaac tggtcggacg ggattggaaa acaggtcatg tatggactat
     7861 atgccattac caattttatt tggacaatca gtttgtatgt ttccaatgcg acaggttact
     7921 tagtacagga agcctattcc ttagacttca tttccgctac agcagattcc attggtaaga
     7981 atatgcagac cttagctggt gtgagtgcaa acggattttc aacagagggt ttctatgttg
     8041 gattcctctt actcttgatt ttggttcttg gggtttatgt tgcctatacg ggactgataa
     8101 agagagaaac cacaaaggca attcatgcca ttatgaattt tgtgctggtg tttatcctat
     8161 cggcttcctt tattgcctac gctcccgact acattaaaaa aatcaatgac ttttcatcag
     8221 acatcagtaa tgccagttta tcacttggca cgaagattgt catgccccat tccgatagtc
     8281 aaggcaagga cagcgtggac ttaatcagag atagcctgtt ttccatacag gttcagcaac
     8341 cgtggctact gcttcaatac aacagttcag acattgaaag tatcggtatt gaccgtgtgg
     8401 aaagcctgct ctccaccagc ccagattcca acaatggcga agacagagaa aaaattgttg
     8461 cggaagaaat tgaagacaga agcaatacca atctaaccat tacaaagacc attaaccgtt
     8521 taggtacagt cttcttccta tttgtcttca atattgggat ttccatattt gtattcctat
     8581 taacaggaat catgattttc tcgcaggtac tttttatcat ctatgctatg tttctgcctg
     8641 tgagctttat tttaagcatg attccatcat ttgatggtat gtcaaaacga gccataacaa
     8701 agctctttaa taccattttg acacgagctg gaatcacatt gattattacg acagcattta
     8761 gtatttcaac catgctctat accttatcgg ctggttatcc gttctttttg attgcttttc
     8821 tacagattgt gacctttgca ggaatctact tcaagctggg cgatttaatg agtatgtttt
     8881 ctctacagag taacgattct caaagtgtgg gaagtcgtgt gatgagaaaa cctcgtatgc
     8941 ttatgcacgc tcacatgcac cgtctacagc ggaaacttgg acgttccatg actactctag
     9001 gggctgggtc tgccattgtt acaggtaaaa aaggacagtc gggttcgggg agttctgcaa
     9061 ggacacaagc agatcactcc cgaccagacg gaaaggaaaa atcaacactt ggaaaacgta
     9121 tcggtcaaac catcggtaca gtagctgata ccaaagacag aatggtagac actgctagtg
     9181 gtttgaaaga acaggttaaa gatttgccga ccaatgcaag atatgcagta tatcaaggaa
     9241 aatccaaagt aaaagagaat gtccgtgatt taaccagtag tatttctcaa accaaagcgg
     9301 acagagccag tggacgcaag gaacagcagg aacaaaggcg aaaaaccatt gcgaagcgtc
     9361 gctctgaaat ggaacaggtc aaacagaaaa aacagcctgc ttcttctgtt catgaaagac
     9421 cgactacaag acaagaacaa tatcatgatg aacagacctc aaaacagtct aatattcaga
     9481 cttcatataa ggaatctcaa caagccaaac aagagcgtcc agcagttaag tccgattttt
     9541 caagtccaaa agtggaacgc caaggcaata ccgttcaaga aaaaaccgtt caaaagccag
     9601 caacttcaac cactacagca gatagaactt cacaacgtcc aatcacaaaa gaacgtccgt
     9661 ctactgttca aagagtacca ctacaaaata caagaagtag accaccaatc aaaaccgcca
     9721 ccattaagaa agtcggtaag aaaccatgaa gttgaaaact ttagtgattg gtggttctgg
     9781 attattcttg atggtcttct cactgcttct gtttgttgcc attttatttt cagatgaaca
     9841 ggacagcgga atttccaata ttcattatgg aggtgtgaat gtttccgcag aagtgctggc
     9901 tcataagcct atggtagaaa aatatgccaa agaatatggc gttgaagaat atgtcaacat
     9961 acttcttgcg attatacagg tggaatcggg cggtactgcg gaagatgtta tgcagtcctc
    10021 ggaatccctc ggtcttccac ctaattcatt gagtacagaa gaatccatta agcaaggtgt
    10081 gaagtatttc agtgaattat tagccagtag cgaaaggctc agtgtagatt tagaatcggt
    10141 tatccagtcc tacaattatg gtggtggttt cttagggtat gtggctaatc gtggaaataa
    10201 atataccttt gaactggctc aaagtttctc aaaagagtat tcaggtggcg aaaaagtgtc
    10261 ttaccccaat cccatagcca tacctatcaa tgggggctgg cgatacaact atggcaatat
    10321 gttttatgtg caactggtaa cgcagtatct tgtcacaaca gagtttgatg atgatacggt
    10381 acaagccatc atggacgaag cactgaaata tgagggctgg cgatacgttt acggtggagc
    10441 ttccccgact acttcttttg attgtagcgg actgacacaa tggacgtatg gaaaagctgg
    10501 aattaactta ccacgaaccg cacaacagca atatgatgtg acccagcata tcccactatc
    10561 ggaagcacaa gctggcgatt tggttttctt tcattctacc tataacgctg gctcttatat
    10621 tactcatgtt gggatatacc ttggcaataa ccgtatgttt catgcaggcg acccaatcgg
    10681 ttatgccgac ttaacaagcc cctactggca acagcattta gtgggagcag gacgaatcaa
    10741 acaatgagaa aggaagattt aatgatgaaa tttagaaaaa atcagaataa agaaaaacag
    10801 ataccaaagg aaaagaaacc tcgtgtctac tataaggtca atcctcataa aaaggttgtg
    10861 attgccttgt gggtactttt agggcttagt ttcagctttg cgatattcaa gcactttaca
    10921 gctatagata ctcatactat tcacgaaaca actatcatag aaaaggaata cgttgatact
    10981 catcatgtag aaaattttgt agagaacttt gcgaaagtct actattcatg ggagcaatcc
    11041 gataagtcca ttgataatcg aatggaaagt ctaaaaggct atctgacaga tgaacttcaa
    11101 gctctcaatg ttgatacagt acgcaaagat attcctgtat cgtcttctgt aagaggattt
    11161 cagatatgga cggtagagcc aactggcgac aatgagttta atgtaaccta cagtgtagac
    11221 cagctcatta cagagggaga aaatacaaag accgtccact ctgcttatat agtgagtgtc
    11281 tatgtagatg gttctggaaa tatggtactg gttaagaatc cgaccattac caacatacct
    11341 aagaaatcaa gttataaacc aaaagccatt gaaagtgagg ggacggttga ttccattaca
    11401 accaatgaaa tcaatgagtt tttaacgacg ttcttcaagc tctatcctac agcgacagcc
    11461 agtgaacttt cctactatgt gaatgacggg atattaaaac caatcggaaa agagtacatc
    11521 tttcaagaac tggtaaatcc tattcacaat cgtaaggata atcaagtcac ggtatcgctg
    11581 acagtggagt atatcgacca gcagaccaaa gcaacgcagg tatctcaatt tgatttggta
    11641 cttgaaaaga acgggagtaa ttggaagatt atagaataac aaatattggt acattattac
    11701 agctattttg taatcacgta ctctctttga taaaaaattg gagattcctt tacaaatatg
    11761 ctcttacgtg ctattattta agtatctatt taaaaggagt taataaatat gcggcaaggt
    11821 attcttaaat aaactgtcaa tttgatagtg ggaacaaata attggatgtc cttttttagg
    11881 agggcttagt tttttgtacc cagtttaaga atacctttat catgtgattc taaagtatcc
    11941 ggagaatatc tgtatgcttt gtatgcctat ggttatgcat aaaaatccca gtgataagag
    12001 tatttatcac tgggattttt atgccctttt gggcttttga atggaggaaa atcacatgaa
    12061 aattattaat attggagttt tagctcatgt tgatgcggga aaaactacct taacagaaag
    12121 cttattatat aacagtggag cgattacaga attaggaagc gtggacagag gtacaacgaa
    12181 aacggataat acgcttttag aacgtcagag aggaattaca attcagacgg cgataacctc
    12241 ttttcagtgg aaaaatacta aggtgaacat catagacacg ccaggacata tggatttttt
    12301 agcagaagta tatcgttcat tatcagtatt agatggggca attctactga tttctgcaaa
    12361 agatggcgta caagcacaaa ctcgtatatt gtttcatgca cttaggaaaa taggtattcc
    12421 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
    12481 ggatattaaa gagaaacttt ctgcggaaat tgtaatcaaa cagaaggtag aactgcatcc
    12541 taatatgcgt gtaatgaact ttaccgaatc tgaacaatgg gatatggtaa tagaaggaaa
    12601 tgattacctt ttggagaaat atacgtctgg gaaattattg gaagcattag aactcgaaca
    12661 agaggaaagc ataagatttc ataattgttc cctgttccct gtttatcacg gaagtgcaaa
    12721 aaacaatata gggattgata accttataga agtgattacg aataaatttt attcatcaac
    12781 acatcgaggt cagtctgaac tttgcggaaa agttttcaaa attgagtatt cggaaaaaag
    12841 acagcgtctt gcatatatac gtctttatag tggcgtactg catttgcgag attcggttag
    12901 aatatcggaa aaggaaaaaa taaaaattac agaaatgtat acttcaataa atggtgaatt
    12961 atgtaaaatc gataaggctt attccgggga aattgttatt ttgcagaatg agtttttgaa
    13021 gttaaatagt gttcttggag atacaaagct attgccacag agagagagaa ttgaaaatcc
    13081 cctccctctg ctgcaaacga ctgttgaacc gagcaaacct caacaaaggg aaatgttact
    13141 tgatgcactt ttagaaatct ccgacagtga cccgcttctg cgatattatg tggattctgc
    13201 gacacatgaa atcatacttt ctttcttagg gaaagtacaa atggaagtga cttgtgctct
    13261 gctgcaagaa aagtatcatg tggagataga aataaaagag cctacagtca tttatatgga
    13321 aagaccgtta aaaaaagcag agtataccat tcacatcgaa gttccaccga atcctttctg
    13381 ggcttccatt ggtctatctg tagcacagct tccattaggg agcggagtac agtatgagag
    13441 ctcggtttct cttggatact taaatcaatc gtttcaaaat gcagttatgg aggggatacg
    13501 ctatggctgt gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa
    13561 gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat
    13621 tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt
    13681 taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg
    13741 tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc
    13801 tgctcggtgt attcaagaat atcgtagtga tttaacttcc tttacaaatg gacgtagtgt
    13861 ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg
    13921 tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat
    13981 tttatgttgt tatataaata tggtttcttg ttaaataaga tgaaatattt tttaataaag
    14041 atttgaatta aagtgtaaag gaggagatag ttattataaa ctacaagtgg atattgtgtc
    14101 ctgtatgtgg aaataaaaca cgattaaaga taagggaaga tactgaatta aaaaaattcc
    14161 ccctctattg tccgaaatgc agacaagaaa atttaattga aataaagcag ttcaaagtaa
    14221 ctgtgattac agagccagac gcaaagacgc agagccgata aaatgagatt aatacaatct
    14281 cattttatcg gctctttccg ttatgtatgg attcttttaa ttagtcttcg atgtttcttg
    14341 cttcgttgat accgctggct aaagattcca ttaaggatag ttctttgtct gtaaagctat
    14401 ccatgtattt ctctatctgt aatcgtcggg tgctttttac caagttatta gcaggtaaga
    14461 aaaattcatc aacggaaaca tgaagtaacg atacaaggtc ataaagaact tgtatgctgg
    14521 ggtgttgccc tttattttca atattagtta agtaccgtgg gtcaatttca atcaatgctc
    14581 ccacttgttc acgagttaaa cctcgtttca atcgagcttc tttaatggct aaaccaaagg
    14641 ctctaaaatc atatttatct tcttttttac gcatagtaga ccacctctat acattttatt
    14701 gttcctactg aattaaaaac aggtatagaa aaacgtgtta tatggtttat aggtttatat
    14761 ttaataaaaa gcactactaa acgccaataa aaaaaaccgt tatatggtag tgctatttac
    14821 gctgttaaaa tattgtatat tacttccaaa tggcggtttg ttggaggtca acgtcgccat
    14881 gaagtacatc atatacaata aatttcctta cattgggttc ttgtcaaaaa aagtcgtcta
    14941 tctgcaatag ataagtacgt ccaccaatgt ggttttataa atcatataga tagaataaca
    15001 gaagcatgta aacagagaaa taaatctgtt tatatgcttt tttggctatt cagaactttt
    15061 ttacaaagtt tatttatcag taatgcaaca aatccccctt tcacattggg actaagagtg
    15121 aaaggagata aacgagcaag gctcacttcc tttcctagac agaaaggggg tgagaaacat
    15181 gaaaccatct tcttttcaga ccacaataga aaatcagttt gactatatct gtaaacgtgc
    15241 tatggaagac gagcgaaaga attatatgct ttatctttca aggattgcaa agcgtgaggt
    15301 gtccttttcg gatgttggcg attatcttgt tagccagttt gcgacaacag ataactattc
    15361 aactgacttt cagattttta cactcaatgg gttatcagta ggcgttgaaa atgatttgtt
    15421 gagtgaagca ttacgtgagt tgccagacaa gaaacgtgaa attctactgc tgttttactt
    15481 tatggacatg agcgattcag aaattgcaga cctgttgaaa ttgaaccgtt ctactgtcta
    15541 tcggcataga accagtggac tagccttaat taaaaagttt atggaggaat ttgaagaatg
    15601 aaaacacaat atcctatgat tccctttcct ctcattgtaa aggcaacaga tggcgatacc
    15661 gaagcgatta accagattct acatcattac agagggtaca taacgaagcg ttccctacga
    15721 cttatgaaag atgaatatgg caatcaaagt atggtcgttg atgaagtctt acgtggaaga
    15781 atggaaacca gactgattac aaagattttg tcatttgaaa ttaagtaata tcctctctcc
    15841 tttcgtggaa gcgtgctaaa ccattccacg cttcccgaac agggaggttt gttattccac
    15901 caaagcatat tgagctttca atgtgttttg ataggctaac gagccattgt tctttgaaaa
    15961 ctgaataaaa gtaatcgaat acgtttcgat aagaaaagag ccaacggaac taaccgccat
    16021 gacctatctt ataaagatag cgagcgattc atgttagtga tccgagaagc aatctttagc
    16081 aggattgcct gcaacgacat tcttatcgtg ataatgatac tcccatacag tcaatagtcc
    16141 gagcgtgata aaaccgtcgc aggcaatgag tatggctaca tgagaaccat gcaggggtgg
    16201 aactcccgtg agctttgcta aagctgttcg attgctggta aaacaacttt tatgaaatcc
    16261 aaataagtga tttggaaagg aggattttat gaagcagact gacattccta tttgggaacg
    16321 ttatacccta accattgaag aagcgtcaaa atattttcgt attggcgaaa acaagctacg
    16381 acgcttggca gaggaaaata aaaatgcaaa ttggctgatt atgaatggca atcgtattca
    16441 gattaaacga aaacaatttg aaaaaattat agatacattg gacgcaatct agcgtcgcca
    16501 aagggtcttg tatatgataa aatagtatta agtcgtatca aggctctttc cataaaggaa
    16561 aggagcaaat gccatgtcag aaaaaagacg tgacaataaa ggtcgaatct taaagactgg
    16621 agagagccaa cgaaaagacg gaagatactt atacaaatat atagattcat ttggagaacc
    16681 gcaatttgtt tactcgtgga aacttgtggc tacagaccga gtaccagcag gaaagcgtga
    16741 ttgtatctca cttagagaga aaatcgcaga gttacagaaa gacattcatg atggtattga
    16801 tgttgtagga aagaaaatga cactctgcca gctttacgca aaacagaacg ctcaaagacc
    16861 aaaggttaga aaaaacactg aaactggacg caaatatctt atggatattt tgaagaaaga
    16921 caagttaggt gtaagaagta ttgacagtat taagccatca gacgctaaag aatgggctat
    16981 tagaatgagt gaaaatggtt atgcttatca aaccatcaat aactacaaac gttctttaaa
    17041 ggcttcattc tatattgcta tacaagatga ttgtgttcgg aagaatccat ttgactttca
    17101 actgaaagca gttcttgatg atgatactgt ccctaagacc gtactaacag aagaacagga
    17161 agaaaaactg ttagcctttg caaaagctga taaaacctac agcaaaaatt atgatgaaat
    17221 tctgatactc ttaaaaacag gtcttcgtat ttcagagttt ggtggtttga cacttccaga
    17281 tttagatttt gagaatcgtc ttgtcaatat agaccatcag ctattgagag atactgaaat
    17341 tgggtactac attgaaacac caaagaccaa aagtggcgaa cgtcaagttc ctatggttga
    17401 agaagcctat caagcattta agcgagtgtt agcgaatcga aagaatgata agcgtgttga
    17461 gattgatgga tatagtgatt tcctctttct taatagaaag aactatccaa aagtggcaag
    17521 tgattacaac ggcatgatga aaggtcttgt taagaaatac aataagtata acgaggataa
    17581 attgccacac atcactccac atagtttgcg acatacattc tgtaccaact atgcaaatgc
    17641 aggaatgaat ccaaaggcat tacagtacat tatgggacat gctaatatag ccatgacgct
    17701 gaactattac gcacatgcaa cattcgattc tgcaatggca gaaatgaaac gcttgaataa
    17761 agagaagcaa caggagcgtc ttgttgctta gtagtacaaa tgaatttact acttatttac
    17821 cacttctgac agctaagaca tgaggaaata tgcaaagaaa cgtgaagtat cttcctacag
    17881 taaaaatact cgaaagcaca tagaataagg ctttacgagc atttaagaaa atataaaaag
    17941 ataattagaa atttatactt tgttttactt aaaaataaat gttctttttc atttattgat
    18001 ccac
//
LOCUS       DATROY010000025        17850 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.025, whole genome shotgun sequence.
ACCESSION   DATROY010000025 DATROY010000000
VERSION     DATROY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17850
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..832
                     /locus_tag="VBD11_000740"
     CDS             <1..832
                     /locus_tag="VBD11_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6953711.1"
                     /translation="LEADIEDNFYSSDLALKSLFYNNKTLRLPKYGTASLVESENSFT
                     AYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAFEPRHKVLAFDYTQTYENITRSQ
                     VEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLFGAFAHSLLFTEIREKQGLAYTI
                     GSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNIKMGRFSSTLLKQTKDILKMNYV
                     LASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVTKSDIVSVATKLKLQAFYFLEGN
                     "
     gene            834..2117
                     /locus_tag="VBD11_000741"
     CDS             834..2117
                     /locus_tag="VBD11_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6953712.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            2240..2782
                     /gene="pgsA"
                     /locus_tag="VBD11_000742"
     CDS             2240..2782
                     /gene="pgsA"
                     /locus_tag="VBD11_000742"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6953713.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            2782..3621
                     /locus_tag="VBD11_000743"
     CDS             2782..3621
                     /locus_tag="VBD11_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6953714.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            3597..4439
                     /locus_tag="VBD11_000744"
     CDS             3597..4439
                     /locus_tag="VBD11_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6953715.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            4432..5226
                     /locus_tag="VBD11_000745"
     CDS             4432..5226
                     /locus_tag="VBD11_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6953716.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            5442..5981
                     /locus_tag="VBD11_000746"
                     /pseudo
     CDS             5442..5981
                     /locus_tag="VBD11_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            6087..6791
                     /locus_tag="VBD11_000747"
     CDS             6087..6791
                     /locus_tag="VBD11_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6953717.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(6926..7798)
                     /gene="sdaAA"
                     /locus_tag="VBD11_000748"
     CDS             complement(6926..7798)
                     /gene="sdaAA"
                     /locus_tag="VBD11_000748"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6953718.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(7813..8481)
                     /gene="sdaAB"
                     /locus_tag="VBD11_000749"
     CDS             complement(7813..8481)
                     /gene="sdaAB"
                     /locus_tag="VBD11_000749"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6953719.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            8727..9848
                     /gene="mnmA"
                     /locus_tag="VBD11_000750"
     CDS             8727..9848
                     /gene="mnmA"
                     /locus_tag="VBD11_000750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6953720.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            9880..10491
                     /locus_tag="VBD11_000751"
     CDS             9880..10491
                     /locus_tag="VBD11_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6953721.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            10661..12562
                     /gene="mnmG"
                     /locus_tag="VBD11_000752"
     CDS             10661..12562
                     /gene="mnmG"
                     /locus_tag="VBD11_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6953722.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            12629..14611
                     /locus_tag="VBD11_000753"
     CDS             12629..14611
                     /locus_tag="VBD11_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6953723.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            14617..15069
                     /gene="rplI"
                     /locus_tag="VBD11_000754"
     CDS             14617..15069
                     /gene="rplI"
                     /locus_tag="VBD11_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6953724.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            15112..16467
                     /gene="dnaB"
                     /locus_tag="VBD11_000755"
     CDS             15112..16467
                     /gene="dnaB"
                     /locus_tag="VBD11_000755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6953725.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            16479..16766
                     /locus_tag="VBD11_000756"
     CDS             16479..16766
                     /locus_tag="VBD11_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6953726.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            17096..17707
                     /gene="rpsD"
                     /locus_tag="VBD11_000757"
     CDS             17096..17707
                     /gene="rpsD"
                     /locus_tag="VBD11_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6953727.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT         5862 a         2819 c         3480 g         5689 t
ORIGIN      
        1 tttagaggcc gatattgaag ataattttta tagcagtgac ttagcactaa aatctttatt
       61 ctacaataat aagacacttc gtttacctaa gtatggtaca gcatctttag tggaatcaga
      121 aaattcattt acagcttatc aagaatttca gaagatgctt aaggaagatc aattagatat
      181 ttttgtcgta ggagattttg atgactatcg aatgattcag gcatttaatc gtatggcatt
      241 tgagccacgt cacaaagtat tagcttttga ttatacgcag acttatgaaa atatcacgag
      301 aagtcaagta gaagataagg acgttaatca atctattatg caattggctt atcatcttcc
      361 aataacttat aaagatgaag attattttgc tttaatagta tttaacggtt tatttggtgc
      421 ttttgcccat tccttgttgt ttacagaaat tcgagaaaag caaggtttag cctatactat
      481 aggtagtcaa tttgacagct ttacaggtct ctttacgata tatgcaggta ttgataaaga
      541 gaatcgtgag cgttttttga agcttattaa taagcaattc aataatatta agatgggcag
      601 gttctcttct acattattga aacaaacaaa agatatattg aaaatgaact atgttttagc
      661 ttctgataat cctaaggtca tagttgacca catttatcat gaacattatt tagatcagtt
      721 tcatacttca gcactattca ttgacaaagt ggacgatgta acaaaatcag atattgtcag
      781 cgtagcaaca aaattaaaat tacaagcttt ttattttttg gaaggaaact aggatgacag
      841 ttaataaaat aacttatcag aaccttcagg aggaagtata taagttaaca ttagagagcg
      901 ggctaaatgt atatcttatt ccaaaaccat catttaagga aactgttgga gtattgacag
      961 caaattttgg atcactccat acaagatata cgaggaatgg ttgtgtagaa cattatccgg
     1021 caggaattgc tcacttctta gaacataaat tatttgaact ggataaagga caagacgctg
     1081 caactcaatt tactaaatat ggcgccgaga gtaatgcctt tacaactttt gataaaacta
     1141 gtttttattt ttcaactata agtcacatta caaactgctt agatatacta cttgactttg
     1201 tattgacaac aaactttaca gaagagtcaa ttactaaaga aaaagacatt atcaaacaag
     1261 aaattgaaat gtatcaagat gacccagaat ataggcttta tcaaggagtg ttatcgaacc
     1321 tttaccccaa ctctccatta gcatttgata tcgctggaga ttaccaatca atctctcaaa
     1381 taaccttaac cgatttacaa gaaaatcaca aagactttta tcagttgtct aatatgaatt
     1441 tagttcttgt tggacaattt agtccacaag aaattataac ttaccttcaa aaaaattctc
     1501 atttaacaaa ttactcacag aatattgacc gagattctat tagtcttgaa cctgttataa
     1561 aaaacaactc ttgccatatg acagtgacaa aacctaaatt agctattggt tatcgaaaat
     1621 caagtcatat gagacatggt tcttacctaa aagagaaaat cggattacag ctattttttg
     1681 caatgctttt aggatggact tctacaatta atcaagactg gtacgaatct ggtcaaattg
     1741 atgactcttt tgatatagaa attgaagttc atccagattt tgagtgcgtt attatttctt
     1801 tagatacgac agagccaatt gctttttcaa ctcagttgag gttattgcta aaaaatgctt
     1861 tgcaatcatc tgatcttacc gaaagccact tacaaaacgt taagagagag ctctatggcg
     1921 attttttaag gagcttagat tctattgaaa atctcgcaat gcaattcgtc acctatttat
     1981 atgatggaaa aacgatgtat ttagatttgc catctatcgt tgaagaatta gacttggaag
     2041 atgtcattac gattggtaag gatttcttag ataatgctga cacatctgac ttcgttattt
     2101 ttccaaaatc ttcgtaaata atctgaaatt ttgatataat aaggaagctt agacatctat
     2161 aaagttggta tgtcaaaagt cttttactag ttttgaaata acataatata ggaatactat
     2221 gtttccaaaa ggagaattta tggtgaagaa agaaaatatt ccgaatctat taactgtagt
     2281 cagaatttta atgattccgt tatttatagt attaacttca gtaacaacaa gtacaacttg
     2341 gcatattgtt gcagctattg tatttgctat tgcaagcttg accgattacc tagacggtta
     2401 tctagcacgt aaatggcaag tagtaacaaa ttttgggaaa tttgcagatc cattagctga
     2461 taaaatgcta gttatgagtg cttttatcat gttagttgga ctagacttag caccggcttg
     2521 ggtttcagct attatcattt gtagagagct agcagttaca ggtttgcgtt tacttttagt
     2581 tgaaacaggt ggaacagtac ttgctgcagc tatgccaggg aaaataaaga cggctactca
     2641 aatgtttgca gttatttttt tgttagtaca ttggatgaca cttggtaaca tcatgttata
     2701 cattgcttta ttctttacct tgtattcagg ctacgactat tttaaaggtg caggcttctt
     2761 atttaaggat acttttaaat aatgacaaat attattactg ttaataatct cttttttaaa
     2821 tatgatagta atcaaacaca ttatcaattg gagaatgtat cgtttcatgt gaaacaagga
     2881 gaatggctat ctattatcgg tcataatggt tctgggaaat ccactactgt tcgtttaata
     2941 gatgggcttt tagaggcgga gtcaggccag attatcattg atggacaaga gttaaccgaa
     3001 gataatgtct gggagttacg tcacaagatt ggcatggtat ttcaaaatcc tgataaccaa
     3061 tttgtagggg caactgttga agatgacgtc gcctttggtt tggaaaataa aggtattcct
     3121 ttgaaagaca tgaaagaaag agtggaccaa gcacttgatt tggtaggcat gtctgaattt
     3181 aaaatgaggg agcctgcacg tttatctggg ggacaaaagc agcgtgtagc cattgcagga
     3241 gctgtagcta tgcgtccaca agttatcatc ttggatgagg cgactagtat gcttgatcct
     3301 gagggacgat tggaactgat aagaacgata cgagctattc gtcaaaaata taatcttact
     3361 gttatttcaa ttacacacga cttagatgaa gttgcattga gtgatcgtgt catcgttatg
     3421 aaaaatggta aagttgagtc gacatccact ccaaaagcgt tatttggtcg tggaaatcgt
     3481 ttgattagtt taggtttaga tgttcccttt acaagtaggt taatggcaga actggctgct
     3541 aatggtcttg atatagggac agagtatctt acagagaagg aattagaaga acaattatgg
     3601 gaattgaatt taaaaatgta agttatacct atcaagccgg cactcctttt gaagggcgtg
     3661 ccctttttga cgtcaatctg aaaattgaag atgtttccta taccgcgttc attgggcaca
     3721 caggttctgg aaaatcaact attatgcaac ttttgaatgg tttacatatt cctacaaaag
     3781 gtgaggtaat tgtcgatgat ttttctatta aagcagggga caagaacaaa gaaatcaaat
     3841 ttataaggca aaaagttggt ttagtttttc aatttccaga aagtcagctt tttgaagaga
     3901 cagttttaaa ggatgttgct tttggaccac aaaattttgg tatttctcag attgaagctg
     3961 aaaggctggc tgaagaaaaa ttaaggttag ttggtatcag tgaggattta ttcgataaaa
     4021 atccatttga actttctgga gggcagatga ggcgggttgc tatagctggt attttagcga
     4081 tggaacccaa agtactagta ctggatgagc caacagctgg acttgatcct aagggaagaa
     4141 aagaattaat gactcttttt aaaaatcttc ataaaaaagg aatgactatc gtcttagtga
     4201 ctcacttaat ggacgatgta gcggattatg ctgactatgt gtatgtttta gaagcaggga
     4261 aagtaacctt atcaggacaa ccaaaacaga tttttcaaga agtagaactt ttagaaagta
     4321 aacaattagg agttcccaaa atcaccaagt ttgctcaaag actatctcat aagggattaa
     4381 atttacctag tttaccaatt actattaacg aatttgtgga ggctattaag catggataaa
     4441 ttgattttgg gacgttacat accaggtaat tctctcattc ataaattaga tcctcgaagt
     4501 aagttactag ccatgttgct ttttatcatt attgtttttt gggcaaataa tgtagtgaca
     4561 aatgtgatcg tattcatttt tacgttggtt attgtgggtt tatctcagat aaaattttcc
     4621 tattttttca atggtattaa accgatggtt ggtattattt tatttacaac cctgttccag
     4681 atgctgtttg cacaaggcgg gcaggttatc ttctcgtttt ggatttttag catcactagc
     4741 cttgggttac aacaagcagc acttattttt atgagatttg ttttaattat cttcttttcg
     4801 acattactta ccttaactac tactccgtta agtttagcag atgctgttga atctttgtta
     4861 aaaccattgg aagtactgag agtaccagca cacgagattg gtttgatgtt atcccttagc
     4921 ttacgtttcg ttcctacttt aatggatgat acaacacgta tcatgaatgc tcaaagagct
     4981 cgtggagttg attttggaga gggaaaccta attcataagg ttaagtccat tatccctatt
     5041 ttgattccat tatttgcttc tagttttaaa agagcagatg cgttggcaat tgctatggaa
     5101 gcaagaggtt atcaaggtgg agccaatcgt agtaagtacc gtttattgaa gtggactgtt
     5161 cgggatactt ttagtatatt actgatgtta ttattgggtt tgagtttgtt tctattaaaa
     5221 aattaaaagg ttaaggtcag tttatggtct tagccttttc atttatataa tgtgaaaagc
     5281 tcaacaactt tacttaaaga aaactaaaaa ttgttttttg gaggcctttt ctttattaat
     5341 ttacaattta tttgtaataa atttcatcta aggcaaaaca attagtaata tttacaaggt
     5401 atgatagtac gcgtatcaat taatgaaaga gagcatatat tatgtcaata acctcggtta
     5461 aaaaatcaaa accatttaaa ttaggagtgg caggtctttt agtgggtgct tcattagctt
     5521 taccactttc agtaagcgca gcatcttata ccgtgaaatc aggtgatacc ttatcagcta
     5581 ttgctaaaaa tcataaaact acggtacaat agttagtgtc tctcaatagt atcagtaacg
     5641 ctgatgtcat cagtataggt gatgttttaa aattggataa ttctacagct agtcaagcag
     5701 aagcaaaatc tcaaccaaca attgaaaatt caatgaattc ttcatcaaat ttgagttcaa
     5761 gtgattcagc tgcaaaagaa gaaatagctc gtcgtgaatc aaatggtagt tatactgcac
     5821 agaatggaca atattatgga agatatcaac tgtctcaatc ttacctaaat ggcgacttat
     5881 ctcctgaaaa tcaagaaaaa gtagcggaca attatgtggc ttctcgttac ggatcttggt
     5941 cggcagcgct atcattttgg aatagtaacg gttggtatta ataaaagttg ataaaacaaa
     6001 gagatttact taaaattaaa tctctttgta atatttttaa catctattca attgcaaggt
     6061 aacaaataaa gtataatata ggtaacatga ataaaagaag aaaattatca aaattgaatg
     6121 taaaaaaaca acatttagct tatggagcta tcactttagt agcccttttt tcatgtattt
     6181 tggctgtaac ggtcatcttt aaaagttcac aagttactac tgaatctttg tcaaaagcag
     6241 ataaagttcg cgtagccaaa aaatcaaaaa tgactaaggc gacatctaaa tcaaaagtag
     6301 aagatgtaaa acaggctcca aaaccttctc aggcatctaa tgaagcccca aaatcaagtt
     6361 ctcaatctac agaagctaat tctcagcaac aagttactgc gagtgaagag gcggctgtag
     6421 aacaagcagt tgtaacagaa aatacccctg ctaccagtca ggcacaacaa acttatgctg
     6481 ttactgagac aacttacaaa cctgctcaac accagacaag tggccaagta ttgagcaatg
     6541 gaaatactgc aggggcggtc ggatctgctg ctgcagcaca aatggctgct gcaacaggag
     6601 tccctcagtc tacttgggaa catattattg cccgtgaatc aaatggtaat cctaatgttg
     6661 ctaatgcctc aggagcttca ggacttttcc aaacgatgcc aggttggggt tcaacagcta
     6721 cagttcagga tcaagttaat tcagctatta aagcttatcg tgctcaaggt ttatcagctt
     6781 ggggttacta gtgaatagct ataaattaac tttacactat tgtcaataat aattacagat
     6841 tgctaaaaca aataaataaa aacgctacag taaagctgta gcgtttttat ttattttcag
     6901 ggaaagagta gaaaattgat aaactttatt ccccaaaaat ttctttgcta taacgtcttc
     6961 ctgtcggcgt ggcagcaagt cctccttctg cagtttcacg aaaagcagtc ggtaaacttg
     7021 atccaacttg atacattgca tcaataactt catctactgg aatttgcgat tcaataccag
     7081 ccaaggccat atcagcagca acaagtgcaa aacttgatcc aagagcattc cgcttcacac
     7141 aagggacttc aactaaacct gcaacagggt cacagataag tccaagcata tttttaataa
     7201 caaatgctat agcttggcta gcttggaaag gagtacctcc agcagccata actaaagcag
     7261 ccgcagccat agcactagct gacccaactt cagcttggca acctccttct gcacctgaga
     7321 tagaggcatt attaccaatg acaagaccaa atgcgccggc tgtaaataga aaattaagtt
     7381 gctcttcttc tgttaaatta agcttttcaa tggctgtaga aatcacagct ggtaaacatc
     7441 ctgcactacc tgcagttggt gttgcacaga ccagtcccat cttagcattt aactcattaa
     7501 cagccatagc attcctaacg gcagctagga ttgtggtatc tgaaatagtt tttcctgatt
     7561 gtaaatattg atccatcttg acagcatcac cgcctgttaa accactgatt gatttactag
     7621 gggtcaatcc atcaataaca gaagctttca taacctcaag gtttcgggac ataatataac
     7681 gaatttcttc acgacttcta ccagtcattt caatttccgt ttggatcatg agctctgcta
     7741 tgttaccctt atgttggcta ttagcttgct ctaccagctc ttcaattgta taaaacatag
     7801 ttaccctttc tcttaatcaa agaagttgac attgtgtaaa tgaggaatag cttctatctt
     7861 tttgacagct tgttgacaat ctcgtgaatc tacttctata atcatgatag ctttttctcc
     7921 tgcactttcg cgtgttacat tcatttgagc aatattaatg ttaaagtcag ataaaatatc
     7981 agttacttta gcaatcatgc caggtatatc ttgatgaacg ataattaaag taggagtatt
     8041 cattgttaat gaaactgaga atccattcaa ctcagtaact tggatgtttc cgccaccaat
     8101 tgacactcct gtaatactca tgctcctatc cccattttta acagttattt tcgcagtatt
     8161 tggatgtggt gaattactat cttttaggat atcccagtaa atcttaattc ctttttgatg
     8221 agcaatttca agagagtttt taatatctgg attatctgta tccattccta gaatccctgc
     8281 aaccaatgct ttatcagtac cgtgtccttg gtaagtttta gcaaaagaat tgtataaatg
     8341 aaaggttact tcactaggtt caccaaaaat agaatggaca actttaccaa tgcggacagc
     8401 tcctgcagta tgactacttg atggtccaat cataacagga ccaataatgt cgaagaccga
     8461 ttgaaatttt aaatgtttca tagtttttcc tcgtgatttt tgtacattct cattataaca
     8521 gattatttct aaaaaatatc ataaaaagat aaaaaaactg caatgtaata aatatttatc
     8581 acaacaaaaa catacaactt gacaacaaat agtgctataa tatataagca cttttatagt
     8641 gtttcttagt caatatgatt gatctaagaa caacttggcc cttcaagcct aagttaaaaa
     8701 taaaactttt tgatgggaga attcttatga ctgataattc aaacattcgt gtcgttgtag
     8761 gcatgagtgg aggtgttgat tcatcagtaa ctgccctgct tttaaaagag caaggttatg
     8821 atgttattgg tgtttttatg aaaaattggg atgacacaga tgaatttggt gtttgtacgg
     8881 ctactgaaga ttataaagat gttgctgctg ttgctgatca gatcggcata ccatactact
     8941 ctgtcaactt tgaaaaagag tattgggaca gggttttcga atatttttta gcagaatatc
     9001 gagcaggtcg tacgccaaat ccagatgtta tgtgtaataa ggaaattaaa tttaaagcat
     9061 ttctagatta tgcgatgaca ttaggtgctg actatgtagc aacaggtcat tatgcacaag
     9121 taacacgtga tgagaacgga atagttcata tgcttagagg agcagataat aataaggatc
     9181 aaacttattt tctaagtcaa ttatctcagg agcaattgca aaaaacgtta tttccacttg
     9241 gtcatttaca aaaaccagaa gttcgccgta tagctgaaga agcaggactt gcgaccgcta
     9301 agaaaaaaga ctctactggt atttgtttta ttggagaaaa aaatttcaag gatttcctgg
     9361 gtcaatattt accggctcaa ccaggtcgta tgatgactgt tgatggtcgt gacatgggtg
     9421 aacatgctgg tttaatgtat tatactattg gtcaaagagg gggccttggt attggtgggc
     9481 aacatggtgg agataataaa ccttggtttg tagttggaaa ggatttaagt aaaaacattc
     9541 tttatgtagg tcaaggtttc tatcatgact cattgatgtc aactagcttg acagcttctg
     9601 aaatccactt tacacgtgat atgcctaatg aatttaagct agaatgtaca gctaagttca
     9661 gatatcgtca acctgacagt aaagttacag tttacgtaaa aggtaatcaa gcaagggttg
     9721 tgtttgatga tttacaacga gcaatcacac caggtcaagc tgttgttttt tataatgaac
     9781 aagaatgttt aggcggtggg atgattgatc aggcttatcg tgatgataaa atctgtcaat
     9841 acatttaatt gacacttaat tattaagtga ggtaatcgta tgatttcaaa atttatttta
     9901 gctttcatgg cttttttcgc cattatgaat cccatttcta atttgccagc ttttatggct
     9961 ttagtagctg atgatgatca gaagatttca cgtcggattg cagcaaaagg agtattactt
    10021 gcttttgtca ttattgtaat ctttgtatta agtggccact tactttttaa cttatttggg
    10081 attaccctag ccgccctaaa aatatctggt ggaatattgg taggcattat aggttataaa
    10141 atgattaatg gtatacattc accaaccaat aaaaacctag aggagcataa ggatgatcca
    10201 atgaatgtgg cagtttcacc attagcaatg ccccttttgg caggtccggg tactattgca
    10261 actgctatgg ggttatcaag tggtggttta tctggcaaac tcattactat acttgctttt
    10321 gccatattat gtgtgataat gtatgttatt ttaataagtg caaacgaaat tactaaattt
    10381 cttggaaaaa atgcaatgac tataatcacc aaaatgatgg gacttatttt gatgactatc
    10441 gggatagaga tgctaataac aggcataaaa attggttttc atttaacata gcctatcccc
    10501 tctggttgac ttttaaaaga taaatgctaa aataaattta acaataacta tgatggtcaa
    10561 agctcatgga atttgtaagt ctttttgacg tacaattttc gagagttttg gctatttttg
    10621 tgttttaatg aattagactt tgaaattgga aaggaataaa atgacacata attttgcaga
    10681 aaactatgat attatcgttg ttggagcggg tcatgcaggt gtggaagcta gtctagctgc
    10741 tagtcgtatg ggatgtaaga cacttctagc cactatcaac ttagaaatgt tagcatttat
    10801 gccttgtaat ccttctatag gtggctctgc taaaggtatc gttgtcagag agattgacgc
    10861 tttaggtggt gaaatgggta aaaacattga taaaacctat atacaaatga aaatgcttaa
    10921 tacaggtaag gggcctgctg tccgtgcctt aagagcacaa gctgataaag ccctctatgc
    10981 acaaacaatg aagcaaactg tagaaaagca agaaaatctt accttaagac aagctatgat
    11041 tgacgaaata cttgttgaag atggaaaggt tgtgggggtt cgtacagcaa ctaatcagaa
    11101 attttcagca aaatctgttg ttataacaac aggtactgct cttcgaggag agattatatt
    11161 aggtgatctt aagtactcct cgggccctaa taatagttta gcttctgtga cattggcaga
    11221 taatttaaga gacctaggat tagaaattgg tcgtttcaaa actggaacac ctcctcgtgt
    11281 aaaagcctcc tctattaatt atgaaaagac agagatacaa cctggggatg agcaaccaaa
    11341 tcatttttca tttatgtcta gggatgaaga ttatattact gaccaggtac cgtgctggtt
    11401 gacctatacg aatactttga gtcatgacat catcaatcaa aatctacacc gtgctcctat
    11461 gttttctgga atcgttaagg gagttggtcc tagatattgt ccgtcaattg aagataaaat
    11521 tgtgcgtttt gctgataaag agcgccacca actcttctta gaaccagaag gtagatacac
    11581 tgaagaagtt tatgttcaag ggctatcaac gagtcttcct gaggatgttc aagttgattt
    11641 attacgttct ataaaagggc ttgaaaatgc tgaaatgatg cgtacaggct atgctattga
    11701 gtatgatatt gtattgcctc atcaattaag agcaacactc gaaacaaaag taattgctgg
    11761 tttgtttact gcaggacaga ctaatggtac ttcaggttat gaagaagctg ctggtcaagg
    11821 gttggttgct gggatcaacg ctgctcttaa agttcaaggg aagccagaac ttattttaaa
    11881 acgaagtgat gcttacattg gtgtcatgat tgatgattta gttaccaaag ggactctaga
    11941 accatatcgt ctgttgacaa gtcgcgcaga atatcgtctg attcttcgtc atgataatgc
    12001 agatatgcgt ttaacagaaa tcggttatga gattggccta gtagatgaag agcgttatgc
    12061 gatatttaaa aagcgtcaaa tgcaatttga aaatgaatta gaacgtttag actctataaa
    12121 gttaaagcca gtttctgaga caaataaacg gattcaagaa ttgggcttta aacctttgac
    12181 agatgcttta actgccaaag aatttatgcg ccgcccacaa atcacatatg cagtagcaac
    12241 ggattttgtt ggttgtgctg atgagccatt agacagtaag gttattgaac ttttggaaac
    12301 tgaaattaaa tacgaaggct atattaaaaa ggctttagat caagttgcaa aaatgaaacg
    12361 aatggaagaa aaacgtattc cccctcatat tgactgggac gacattgatt ctatcgcaac
    12421 agaagctaga caaaaattca aaaaaattaa cccagaaacg ctaggtcagg ctagtcgtat
    12481 ttcaggagtt aatccagccg atattagtat tttaatggtt taccttgaag gaagacaaaa
    12541 aggtcgtaaa aatatcaatt gataatagtg ttcgcccctg tttatcaggg gcgttttgtg
    12601 ttataattag gtcttaagag gttatttaat gaaaagattt cgttttgcaa ccgtccattt
    12661 agttttaata ggcctgattt tatttggact attagctatt tgtgttaggt tatttcaatc
    12721 ctatacggct ctattattgg ctatttttgt tgctttatct tttgttgttg ccttattata
    12781 ttaccaaaag ataacttatg aactatctga agttgaacaa attgaattac ttaatgatca
    12841 aacagaagtt tctcttaaga gtcttcttga acaaatgcca gttggtgtta tacaatttga
    12901 tcttgaaaca aatgatattg agtggtttaa cccttatgca gaattgattt ttactgggga
    12961 taatggtcat tttcagtctg ctactgttaa agatatcatt actagtagac gtaatggaac
    13021 ggctgggcaa tcatttgaat acggtgataa caaatattca gcttatttgg atacagagac
    13081 gggtgttttc tatttcttcg ataatttcat ggggaataga agaaactatg atagttcaat
    13141 gctacgtcca gttattggta ttatttccat tgataactac gatgatatta tggatactat
    13201 gttagaggct gatatgtcaa agattaacgc cttcgtgact agttttattt ctgattttac
    13261 acagtctaaa aatatttttt atcgtcgtgt caatatggat cgatactata tttttaccga
    13321 ctatagcgtt ttaaatacat tgataaaaga taaatttgat attttaaatg agtttcgaaa
    13381 acgcgctcag gaaaaccatt tatcattaac attaagtatg ggaatttctt atggtgatgg
    13441 taaccataat caaattgggc aaattgcttt agaaaatctt aatacagcac tcgttcgcgg
    13501 aggagatcaa atagtagtac gtgaaaatga tagtagtaaa aaagcacttt actttggtgg
    13561 tggtgctgtt tcaactatta aacgttcacg aacacgtacc agagctatga tgactgctat
    13621 ttctgaccgt ttgaaagtag ttgatagtgt ctttattgtg ggacacagga aattggacat
    13681 ggatgcacta ggtgcatctg tagggatgca attttttgct agtaatattg ttaatgccag
    13741 ttatgtagtt tatgacccaa atgacatgaa ttctgatgtt gaaagagcaa ttgattactt
    13801 gcaagaagac ggcgaaacac gtctcgtaag tgttgagaga gcttttgaat taattactca
    13861 aaattcttta ttagtcatgg tagaccattc taaaacagct ttaaccttat ctaaggaatt
    13921 ctttaacaag tttgcagatg ttattgtggt agatcatcat agaagagatg aagattttcc
    13981 aaaaaatgct gttctttcat ttatagaaag tggcgcaagt agtgccagcg aattagttac
    14041 agaattgatt caatttcaac aagctaaaga taaattaagc cgcagtcaag caagtatttt
    14101 aatggctggt atcatgcttg atacaagaaa ttttgcatct aatgtaacga gtagaacttt
    14161 tgatgtggca agctatttac gcggtttagg tagtaatagt atggctattc aaaaaatatc
    14221 agctacagat tttgatgaat atcgtttaat taatgaattg attttaaaag gtgagcgtat
    14281 ttatgataac atcattgttg ctaccggaga agaacataaa gtttactcgc atgttatagc
    14341 tagtaaagca gctgacacca tgttgacaat ggcaggtatt gaggctactt ttgttattac
    14401 taaaaacagt tcaaatattg gtatttcagc gcgcagtaga aataatatca atgtccagcg
    14461 tattatggaa aaacttggtg gtggcggaca ttttagtttt gctgcttgtc aaatacaaga
    14521 caagtctgta aagcaagtta gacgtatgtt actggaaata attgacgaag atttaagaga
    14581 aaatagtaca gtcgaaaata gaagggatta attgagatga aagttatttt tttacaagat
    14641 gttaagggga aaggaaaaaa aggtgaggtt aaggaagtac ctactggtta tgctcaaaac
    14701 ttcttattaa aaaagaatct agctaaagaa gcaacgacac aagctatcgg tgaattaaaa
    14761 ggaaaacaaa agtccgaaga aaaagcacaa gcagaaattt tagcacaggc aaaggagctg
    14821 aaaacacaat tagaatccga aactacccga gtgcaattta ttgaaaaggt aggtccagat
    14881 ggtcgtacgt ttggttctat cacagccaaa aagattgctg aagagttaca aaaacaatac
    14941 ggcattaaaa ttgacaaacg tcatattgat ttagatcata ctattagagc gattggtaag
    15001 gttgaagtac cagtaaaatt acataaacag gtttcttcac agattaagct tgatatcaaa
    15061 gaagcgtaaa ctgagagatg ataaaggttg acaagtcagg aggaatgaag tttggtagaa
    15121 gtatctgagc taagagtaca acctcaagac ctgttggcag aacaggccgt tctaggatct
    15181 atctttatct caccagagaa actgattatg gtgagagaat ttataagtcc agatgatttt
    15241 tataaatatt cacacaaagt tatcttcaga gcgatgatta ccttagctga tagaaatgat
    15301 gcaattgatg ctgctactgt tagaaatatt ttagatgacc aaggtgatct tcaaaacatt
    15361 ggtgggttgg gctatattgt tgaattagtt aatagtgttc caacaagtgc taatgcggag
    15421 ttttatgcca aaatagtttc tgagaaagcc atgttgcgag atatcatttc taagttgaca
    15481 gatactgtca acatggccta tgaaggaaat gattctgatg aaattattgc caccgctgag
    15541 aaggctttag ttgatattaa cgaacacagt aatcgtagcg ggtttagaaa gatatctgat
    15601 gttttaaaag ttaattacga aaacttagaa ttacgttcac agcaaacctc agatgtcaca
    15661 ggtttaccaa ctggatttag agatttagac aggattacta caggtttaca tccagatcaa
    15721 ttaatcatct tagcggctcg tcctgcagta ggtaaaacag cctttgtttt aaatattgct
    15781 caaaatgttg gaactaaaca aaatagacct gtagctattt tttcacttga aatgggggct
    15841 gagagtttag ttgaccgtat gttggcagca gagggaatgg ttgattctca tagtttacga
    15901 actggtcagt tgacggatca agattggaat aatgtaacga ttgctcaagg agctttggcg
    15961 gatgcgccaa tttatattga tgatacgccc gggattaaaa ttacagaaat tagagcacgc
    16021 tctaggaagt tatctcaaga agttgatgat ggtttaggtt taatcgtcat tgactacctc
    16081 caattaattt caggaactag accagaaaat cgtcagcaag aagtttctga aatttcaagg
    16141 cagttgaaga ttttagctaa agaattaaag gttcctgtta tcgcattaag ccagctctct
    16201 cgtggggttg agcaaaggca ggataaacga ccggtcttat ctgatattcg tgaatctgga
    16261 tcgattgaac aagatgctga cattgttgct ttcttatatc gagatgatta ttatcgtaga
    16321 gagggcgaag aagcagagga aattgtagaa gataatactg ttgaggttat tttagaaaag
    16381 aaccgtgctg gtgcgcgtgg gacagtaaaa ttaatgtttc aaaaggaata taataaattt
    16441 tctagtatag ctcaatttga agaataggag atgttaatat gagtgatgca tttgcagatg
    16501 ttgcaaaaat gaaaaaaata aaagaagata tcaagtcaca tgagggacag atggtcgaac
    16561 ttactctcga aaatggtcgt aagagggaaa aaaataaaat tgggcgccta attgaagttt
    16621 acccatcttt gtttatcgta gagtataagg atactgcagc tgtaccagga gcaattgata
    16681 atacttatgt agagtcttac acttattcag atattttaac tgagaaaact ttaattcgtt
    16741 attttgatga cgagtcagca gaatagtatg aaaacctcga ctgaggtttt ttgtttctat
    16801 aaaaaaattt actaattctc tattggtggt tagaattaag aatctttctt attataattg
    16861 gaaatactta ttagatgtct atttgtattt aaggtttaag agacataaaa acttgattca
    16921 atattatttt catgatataa tatagcttgt gtgaaataac agcagaaaaa tatgaagctc
    16981 gtcaacaggt ggttaactaa tctagtaccc tttgctgttt aggcgaaaat cccctgcacg
    17041 aatcaggatt ttttaaatcg ttatttcctc aattatttta gaggaggaca tttagatgtc
    17101 acgttatact ggtccatcat ggaaacaatc acgtcgcctt ggtttatcac ttacaggcac
    17161 aggtaaagaa ttggcacgtc gtaactacgt acctggtcaa cacggtccta ataaccgtag
    17221 caagctttca gaatatggct tgcagttagc tgagaaacaa aaacttcgtt tttcatacgg
    17281 acttggtgaa aaacaattcc gtaacttgtt cgtacaagct actaaagcaa aagaaggaac
    17341 tcttggtttc aactttatgg ttcttttaga gcgtcgtctt gataacgttg tttatcgttt
    17401 aggacttgca acaactcgtc gccaagctcg tcaatttgtt aaccatggtc atatccttgt
    17461 tgatggtaaa cgtgttgata tcccatcata ccgcgttact ccaggacaag ttatttcagt
    17521 tcgtgaaaaa tcaatgaaag tacctgcaat ccttgaagct gttgaagcta ctttaggacg
    17581 tccagctttc gtatcatttg atgctgaaaa acttgaaggt tcattgactc gccttccaga
    17641 acgcgatgaa attaacccag aaatcaatga agcacttgtc gttgaattct acaacaaaat
    17701 gctttaatta ttacattaca tactttaaaa gcctatttta taggcttttt ttgctatata
    17761 ctttttagtg agtcattata tttaatgaac caaaaagatt ttaaactaac attagaataa
    17821 aaatcaacac tgtcagtttg taatgagtaa
//
LOCUS       DATROY010000026        17687 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.026, whole genome shotgun sequence.
ACCESSION   DATROY010000026 DATROY010000000
VERSION     DATROY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..156
                     /locus_tag="VBD11_000758"
     CDS             <1..156
                     /locus_tag="VBD11_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6953728.1"
                     /translation="YGSYLLAKGVDIWVVARLMGHKDITQLLETYGHVLTEVINKEYE
                     TVRSLVS"
     gene            complement(257..329)
                     /locus_tag="VBD11_000759"
     tRNA            complement(257..329)
                     /locus_tag="VBD11_000759"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:Lys,seq:ctt)
     gene            complement(611..1879)
                     /locus_tag="VBD11_000760"
     CDS             complement(611..1879)
                     /locus_tag="VBD11_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6953729.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(1872..2363)
                     /locus_tag="VBD11_000761"
     CDS             complement(1872..2363)
                     /locus_tag="VBD11_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6953730.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            2690..3160
                     /locus_tag="VBD11_000762"
     CDS             2690..3160
                     /locus_tag="VBD11_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6953731.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            3296..3754
                     /locus_tag="VBD11_000763"
     CDS             3296..3754
                     /locus_tag="VBD11_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6953732.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            3726..4196
                     /locus_tag="VBD11_000764"
     CDS             3726..4196
                     /locus_tag="VBD11_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6953733.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(4235..4951)
                     /locus_tag="VBD11_000765"
     CDS             complement(4235..4951)
                     /locus_tag="VBD11_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6953734.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYDYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            5075..5533
                     /locus_tag="VBD11_000766"
     CDS             5075..5533
                     /locus_tag="VBD11_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6953735.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            5530..6483
                     /locus_tag="VBD11_000767"
     CDS             5530..6483
                     /locus_tag="VBD11_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6953736.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            6483..7223
                     /locus_tag="VBD11_000768"
     CDS             6483..7223
                     /locus_tag="VBD11_000768"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6953737.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            7220..7806
                     /locus_tag="VBD11_000769"
                     /pseudo
     CDS             7220..7806
                     /locus_tag="VBD11_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
     gene            8007..8888
                     /locus_tag="VBD11_000770"
     CDS             8007..8888
                     /locus_tag="VBD11_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO6953738.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            8903..9748
                     /gene="pstC"
                     /locus_tag="VBD11_000771"
     CDS             8903..9748
                     /gene="pstC"
                     /locus_tag="VBD11_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6953739.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            9750..10628
                     /gene="pstA"
                     /locus_tag="VBD11_000772"
     CDS             9750..10628
                     /gene="pstA"
                     /locus_tag="VBD11_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6953740.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            10621..11370
                     /gene="pstB"
                     /locus_tag="VBD11_000773"
     CDS             10621..11370
                     /gene="pstB"
                     /locus_tag="VBD11_000773"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6953741.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            11367..12023
                     /gene="phoU"
                     /locus_tag="VBD11_000774"
     CDS             11367..12023
                     /gene="phoU"
                     /locus_tag="VBD11_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6953742.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            12023..12700
                     /locus_tag="VBD11_000775"
     CDS             12023..12700
                     /locus_tag="VBD11_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6953743.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            12693..14348
                     /locus_tag="VBD11_000776"
     CDS             12693..14348
                     /locus_tag="VBD11_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6953744.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            14503..16686
                     /locus_tag="VBD11_000777"
     CDS             14503..16686
                     /locus_tag="VBD11_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6953745.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            16725..17555
                     /locus_tag="VBD11_000778"
     CDS             16725..17555
                     /locus_tag="VBD11_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6953746.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
BASE COUNT         5465 a         2926 c         3781 g         5515 t
ORIGIN      
        1 tatggaagtt acttattagc aaaaggtgta gatatttggg tagttgcaag attaatgggt
       61 cataaggata ttacacagtt acttgaaact tatggtcacg tcttgacaga agtaataaat
      121 aaggaatatg aaacggtcag aagtttagta tcttaaagct aatttttaaa gaatatcttc
      181 tagaacaaat ttagaacaaa tctgctgaaa caaagaaaaa aacctttaaa atcaaggttt
      241 ttgacaatat tattaatgcc ccctacaggg ctcgaacctg tgacccatag attaagagtc
      301 tactgctcta ccaactgagc taaggaggct aggaagctgt attggcaccg atgatccact
      361 ttttgtattg atcccgcaca aaataagcag gtgggtaact cgcacctctt taagtggctt
      421 ccgcgtgtta acggcttgcg cactagagat gacttcgttt cccgaaatac aaagaaatag
      481 tcggtcaaca cttagatgtg gattcgtatg ccacagcaat gtttctatgt ctttatgata
      541 ccacttttta taaaattttc aagcaaaatt atcgattttt gaaatcgatt gcgattttta
      601 aaactggcaa tcagtctgag agcttatcaa tgcgctcttt atttgaacca agtgcacgtt
      661 cgtatttacc agtttcattg gcttcaaaat aattatgtcc tactagttta tctggtaagt
      721 attgttgttt aacccatttt tcaggatagg cgtgagggta cttgtaatcc cttgcattgc
      781 ccaaggtttt actaccgctg taatgtccat ctcttaggtg acgtggaata ggtaaatttc
      841 ctgaacgacg taaatcagct aaggctgcat ccatagccaa ataggctgaa tttgatttag
      901 gtgatagagc taaatccaca acaatgttag caataagaat acgagcttct ggaaaaccaa
      961 tgcgttgggc agcttccaat gcagtcacag tatgtatttg tgcttcgggg ttagctaagc
     1021 cgatatcttc ataagcaata atggtcaaac gtcttgctaa actaggtaaa tctccagctt
     1081 ctactaaacg agcggcatag tgcaggctgg cattgacatc agaacctcta attgattttt
     1141 gtaaggctga taggacatca taatgcccat ctccgttttt atccattgta atataactac
     1201 gttgcagact attttccatt gtctctagac taatatgacg gctaccatct tcgttaggag
     1261 aagtagacat gactgctaga tccaaggaat tataagcaga tcgtaggtct ccatttgtgg
     1321 cggtaacaat gaaatctagc gccccatcat caattgtcac taaaaaagga aatccccgtt
     1381 ctttatcgga aattgctagt tgaatggctt ttttaatatc ctcgttagaa agaggttcta
     1441 gctcaaaaat ttgtacccta cttctaatgg caggagttac tgaaaagaaa ggattttcag
     1501 ttgttgctcc aatcataata atattgccat tctcaagtaa aggtaaaaga aaatcttgtt
     1561 ttgtcttgtc aagtctatga atctcgtcaa gtaataaaac taagccacca gaaaatttag
     1621 cttcttctgc aatttcttga agacgttttt tactatcaac agtggcatta aaagtacgaa
     1681 aggcatattt agtagtacca gcaatagcag aagcaataga agtcttccca atacctggtg
     1741 gtccatatag gatcatggaa gataacatat tagctgcaac cattcgatca ataattttgc
     1801 cattaccaac gagatgttgc tgcccaatga cttcattaat attgcgagga cgcatacgta
     1861 gagcaagatt gtcagccatg attattctcc aaatcttttg cgaaaattaa agcatcaaca
     1921 taatgatttt caatgaaaaa ggctttagtg attcgagcct cttcggtaaa accgtatttt
     1981 tgatagaggg aaatggctct actattgttg cccatgacgt gcatggcaat cttgatgtaa
     2041 ccttgagctt tagcctcagt tagcagagct ttcaagagag ccttacctag cccctgacct
     2101 tggtagggtt cagcgatgag aatgccgaaa gtggcaacat gcttgccagc tgggaaagga
     2161 tagtagggac caaagtccaa gacgccagcg atcttatcat cgatctcagc gacaaagaag
     2221 gacatgccat ttgccagatt cttctccaag tcactctcag tccacttgtg gtcatagaca
     2281 ggagaggtaa gagcagtcca gccagtattt tgtaaggtca gaactgcctc acggtcgcag
     2341 ttctgcatag gtctgataat cataattgat gatacaaagg gacggttcgg tttacgaaaa
     2401 ttttcgtaaa gaaataggga acgacgaagt gtcgtcagac actaggaagt tcatcttttc
     2461 ttttagaaaa ttagtcccgt gttcaaatcc tttctatttt ttatatagag tttttctctc
     2521 agctgactct tttaacatag tatgaagtca gtcaaaaacg actagtcttt gagactattg
     2581 taacataaaa ataagtagga actacctttt gattaacctg agtctgtaac gtttttcttc
     2641 tatattcatg ttataataag aaagaataaa tagaaaataa ggacataaga tggctaaatt
     2701 tggattttta tcggtactag aagaagaatt ggataagcat ttacagtatg actttgccat
     2761 ggattgggat aagaaaaatc atactgtaga agtcactttt atattagaag cacaaaacag
     2821 ctctgctatt gaaaccgtgg acgaccaggg ggagatgagt agcgaggaca tcgtttttga
     2881 ggactatgta ctcttttaca accctgtcaa gtctcgcttt gatgctgagg actatctggt
     2941 gactatcccc tatgagccta agaagggctt gtcgcgtgag tttctggctt attttgcgga
     3001 gactctcaac gaggttgcca ctgaagggtt gagcgacctc atggacttcc tcaccgatga
     3061 cagtatcgaa gagttcggtc tttcttggga tacggatgct tttgaaaatg gcagagcaga
     3121 actgaaagag acagagtttt acccttatcc gagatattag acttgatgga tttgctaaaa
     3181 tgtggatata cattggatga tatagaaaca gccagaccag aggatatgga acgttattat
     3241 ccccctgaac aaatcagaaa atatggagct ttaggaatag aattgaggtt attgcatgga
     3301 tatttttgat gagttatttg atttctcagg tgcaaagatc gctcttttct gcgagggaaa
     3361 gattttgacc agcctaagag atgattttcc agatttgccc tatgcaggct tttgggactt
     3421 accaggcgat ggtcgtgagg gcgatgaaac acctttggaa tgtctttttc gagaagttag
     3481 tgaagaatta aatctgacct taactcgaaa ccatattgat tgggtcaaga cttatagggg
     3541 tatgctgaac cctgataaat tatcggtttt tatggttgga catatcagtc agaaagagta
     3601 cgatagcatt gttctaggcg atgaaggaca agactacaag ctcatgtcca ttgacgaatt
     3661 tttaagccat aaaaaggtca ttcctcagtt gcaggagagg ttgagagatt atttggaggt
     3721 ggaagatgat tactttacaa aaggcggaag ctagtgattt agagaagatt attgccattc
     3781 agagagctag ctttaaggca gtctatgaga aataccacga tcaatacgat ccctatgtgg
     3841 aagaagttga gcagattcga tggaaactag tggagcgacc agattgtttc tatcattttg
     3901 tccttgttga tgagacaatt gttggttttc ttagattggt tatcaaggac gaggagaagc
     3961 gtgcttggct tggtacggcg gctattcttc cccaatatca aggacagggc tacggctcag
     4021 cagcgatggc tttattagag aaaacctatc ctaagcttac aaagtgggat ttatgtacga
     4081 ttgcgcaaga aaaactaatg gtcagttttt atgaaaaatg tggttatcat tcaacccata
     4141 ccgaacctga gcaagaagga atggatatgg tctatatgac aaagaagata gaataaaaaa
     4201 agaaaactag atgattgtct agttttctga tagtttattg gcaatagtaa gcaatagctt
     4261 ggctgacaaa ttcacttgtt cctgctccac cagctttgtc aattgattga ctaaaccggt
     4321 catctgattg atacatttgt cctagtccag caaggatttc attggtacag gtgtaaaaat
     4381 gctgagagat atagtcttgt agccgtttca cacacatttg aacagattca tcagatacat
     4441 tttgtgtctt taattgccca aattgtacca ttatttttgc catttcttgg ctaatttgtg
     4501 caaagtcatc tgaagcatgt ttttgagcaa attcttggta ggctgctgtt tgtccccatt
     4561 tttcttttgc ttcagcttgt aattgttcta attctgtttt attataaaca tcgaaattca
     4621 tataatcata tcctttttct tgtaatcgtt tcgcatgggt gataacctgc tcaagatgcg
     4681 atcttttcca ttcaagcaat ttgatttgtt gggctaataa gtcttgcctt tcctcttttg
     4741 cttgctcaag caggtatttg atatctttga gagggaattc caactcacga aatagcaaaa
     4801 tttcttgcag gcgaatcaat gcttcatcgt cataaagtcg atagcctgct tcggataggg
     4861 cggtaggtgg gaataaaccg attttatcat agtgatgtaa ggtgcgcaca cttacaccgc
     4921 ttaagatact gacttcttta actgttttca tggtaataac ctcctctcac ttacatagta
     4981 taaaggataa cctaaggtca gggtcaagca aaaaataaat tattttgaaa agtatggtta
     5041 atcatattga tttgaagata aaggagggat agttatagaa atagttgagc tactaaggga
     5101 atggtattct tcctttgaag ttaaagaaga aaagtggaat gaaaaatatg aaggtatcct
     5161 cataagaatt gaaggcgatg gaaactataa aaggtgtcgt ttagctcata agacagctag
     5221 caagatgggg tattttacag tattttggca gaaagatgga aatggtaaaa atattccttt
     5281 ttatgaacaa gatgaagtgg aagatttgat tattgtcatt aaagatggta ggaggaaagg
     5341 tttgttcatc attcccaaag aggttgcagt gagtaagggt attctttcat ctgctaactc
     5401 tcaaggaaag atggctatgc gattttactc tccttggtgt agcgatttaa atagaacagc
     5461 tttggtgaca cagcgatggc agttgaacta ttttattgat ttaagtaaaa ataatgaagg
     5521 agttactaca tgaacacatg gaatgaacta acggttcacg tgaatcgtga ggcagaggaa
     5581 gctgtctcga atttattgat tgaaactggc agtcaggggg ttgccattag cgactcggct
     5641 gattatcttg gacaggagga tcgtttcggc gagctttatc ctgaggtgga gcaatctgat
     5701 atgatcgcta ttactgccta ttatcctgat acgcttgata ttgaggcggt taaggctgat
     5761 ctggctgatc gtttggctaa ttttgaaggc tttggtcttg cgacgggtag cgtcaacctt
     5821 gacagtcagg aattggttga agaggactgg gctgataact ggaagaagta ctatgagcca
     5881 gctcgcatta cccatgattt gaccatcgtg ccgtcatgga cggactacga ggctaaggct
     5941 ggtgagaaga ttatcaagct ggatcctggt atggcttttg ggactgggac tcacccaacg
     6001 actaagatga gtctttttgc cttggagcaa gtgcttcgtg gtggtgagac ggttatcgat
     6061 gtggggactg gttctggcgt tttgtctatt gctagctctc tcttgggtgc caaggacatc
     6121 tatgcttatg atttggatga tgttgcggtg cgcgtggctc aggaaaatat cgatatgaac
     6181 cctggtactg ataatattca tgtggctgct ggtgacctgc tcaaaggtgt ccagcaagag
     6241 gcagatgtca ttattgccaa tattttggcg gatattttga tccatctgac agacgatgcc
     6301 taccgtttgg tcaaggatga gggttatctc atcatgtctg gtatcatttc tgaaaaatgg
     6361 gacatggtgc gtgagagtgc tgaaaaagct ggtttcttcc ttgagactca catggttcaa
     6421 ggtgagtgga atgcctgtgt tttcaaaaag acagatgata tttcaggagt gattggcggc
     6481 taatgcaaca gtattttgta aatggcaaag cagatgactt tgtgaccatc gaggacaagg
     6541 ataccatcaa gcacatgttc aatgtcatgc gattgacaga agatgaccaa gtggtcttgg
     6601 tttttgatga tgccatcaag cgtctggcaa aggtggtgga cagctctgcc catcgcttcc
     6661 aaattctaga agagctagac agcaatgttg aaatgcctgt ccaagtgacc atcgcctcag
     6721 gttttcccaa gggagacaag ttggattttg tgacgcaaaa ggcaacagaa ttgggtgctg
     6781 cggctatctg gggattccca gctgactggt cagtcgtcaa gtgggacggc aagaaactag
     6841 ctaaaaaaga agataagctg gcaaaaattg ctctgggcgc agcggagcag agcaagcgta
     6901 atcgtcttcc tcaggttcgt ttatttgaga agaaggctga ttttcaagcg gagcttgcag
     6961 gctttgataa gatttttatc gcctatgaag agtctgccaa ggagggtgaa atgtcagcgt
     7021 tagcccagaa tttgcagact gtaaaagcag gtgacaagct cctcttcatc tttggaccag
     7081 aagggggcat ttctccaaaa gaaatcgcag cttttgaaga ggttggagct atcaaggtgg
     7141 gcctgggtcc acgcatcatg cgcacagaaa cagcaccgct ctatgctcta agtgttatta
     7201 gttactcagc ggagttatta tgattattgc tattcaaggt gatatggctg tagggaaaac
     7261 cacagctttg aaaatcattg agaaagttta cccgaaaatc gctattagtt ttgaggatat
     7321 ttcaccagta gttgataaga ttaataaaca tcagcttgat aacaataaat tagacgatta
     7381 ttgtgaaatt caacaactat ttattcagca tgagcgagat agatacgaac ttgttagaga
     7441 taatgaaata gtgttaatgg attatggagc tgaggaaatt gctttctata cacaatactt
     7501 tccaaaggtg gcgggttata gttgacttta ccttctaatt tgttactaga ggtaaatagg
     7561 ctaaaggaat gttttgctga ttatattatt tttctagatg cagatgagac ggtcctatac
     7621 caacgtcaaa aaaatgattt taatagacgg cgtcattttt tcaaatggta tactcatcac
     7681 ctgttgcctg ctaaaaaaca ttatttagac acattttcca atgttattca tattaataca
     7741 aatcatttaa atgctgaaga agtggctgaa aaaatcattg acatcattcg taagttaact
     7801 ctataatggg atagttatac ttcaattatg tggtataaca actgtttcaa gccgacttat
     7861 tggctgaatt accataatag aaaaaaggag tttggataat tttccaactc tttttaattt
     7921 tacattgatt ttacaaaaat cctcaaagag atttgatatt ccttaactat aataaaatca
     7981 aatcaaaaaa tatttaggag ttcatcatga aaaaacacaa aatgctatct cttttggcgg
     8041 tatctggtct tatgggaatt ggcatcctag caggatgctc aaatgattca tcatcttcat
     8101 ctaaagggac aattaacatt gtttctagag aggaagggtc tggaactcgt ggagctttca
     8161 tagagttatt tgggatcgag tcaaaaaata aaaagggcga aaaagtagac catacttctg
     8221 atgcagcaac tgttaccaat tcaacttcag ttatgctaac cactgtctct aaagacccgt
     8281 cagctattgg ttattcttca ctaggttcat taaatagtag cgttaaagtg cttaaaattg
     8341 atggcaaaaa tgcgactgta aaagatatta aatcaggaag ctataagatt tctcgtcctt
     8401 tcaatattgt aacaaaagaa ggtaaggaaa aagaagcaac aaaagatttt attgattata
     8461 tcttaagcaa agatgggcaa gcagtcgttg agaaaaatgg ctatattcca cttgacaatg
     8521 ctaaagcata tcaagcaaaa gtgtcatctg gtaaagttgt tatagcaggt tcaagttcag
     8581 taacgccggt tatggaaaag attaaagaag cctatcataa agtaaataca aaagtagatg
     8641 ttgaaattca acaaagtgat tcttcaactg ggattacaag cgcaattgat ggatcagcag
     8701 atataggtat ggcatcacgt gaattggata agacagagtc atccaaaggg gtcaaagcta
     8761 ctgttattgc aacagatggt attgcagtag ttgttaataa gaaaaataaa gttaatgatc
     8821 tatcaacaaa acaagtaaaa gacattttta ctggaaagac aacgtcttgg tctgacttat
     8881 caaagtaagg taagagtaaa caatgaaaca aacaaaagaa ggtcttatgc aagctgtgtt
     8941 tttcatcacg gcttgcgtca gtgttatatc agctatttta atttgcctct ttctcttctc
     9001 tagtggtctt ccagctatta caaaaatcgg ctggggaaat tttatatttg gtaaagtctg
     9061 gcatcctagt aataatattt ttggcatctt tccaatgatt gtaggttcct tatatgttac
     9121 agcgggagct ttacttttag gaggcccgat aggtatcctt actgctgttt ttatggctta
     9181 tttctgccca gaaaatattt ataggccttt aaagtcagct atcaatttaa tggcgggaat
     9241 tccttcagtt gtttatggtt tctttggact cgttgtcatt gtccctatga taaggcaata
     9301 tattggtggc tttggtatgg gagtcttagc agcttcaatt ttgctaggaa ttatgatttt
     9361 accaacaatt gttagcattt cagaatcttc tttaagagct gttccagaga gctattatga
     9421 aggcggtatt gcccttggtg cctctcatga acgaagtgtc ttctttgctg ttttaccagc
     9481 tgctaaacgt ggtattttag catcggttgt tttgggaatt ggacgtgcta ttggtgaaac
     9541 aatggctgtc atcatggtag cgggtaatca agctgtcttg cctcaatcgc tgacatcagg
     9601 cgttagaact ctaaccacta atattgtcat ggaaatggga tattcttctg gtttgcatcg
     9661 tcaagcctta attggtacag ctgtggtact ctttatcttt atattaatga ttaatatcag
     9721 tttttcagca ttacagaaag aggaataaga tggaaaatat taaccaaaga acgtcaaaac
     9781 aatatttagc atctttaaaa aaagatcccc tttccttctt tctttttgct attgtctatt
     9841 tgggagcaat tctaagtttt gctacaattg catttgttgt tatttatatt ttagttaagg
     9901 ggcttcctca tgtgaatact ggcttgtttg catggactta taatactcag aacgtctcac
     9961 tattaccagc tttcattaac actatcttta tcatcgcctt aactttatta tttgcagttc
    10021 cccttggtat tggtggatca atttatctaa cggaatatgc tagacgtgat aatccttatc
    10081 tcaaaattat ccgtgttgca accgagacat tggcagggat accttcaatt atttatggtt
    10141 tatttggtgc attatttttt gtaaaataca cacacttagg cttatctttg atatcgggtt
    10201 ctctgacttt gagcatcatg attttacctt tgattatgag gacaactgaa gaagctttac
    10261 tatctgttcc ggattcttat cgagaaggag cctttgcgct tggtgctggt aaattgagaa
    10321 ccatatttaa aatcgtatta cctagtgcta tgtctggtat ttttgctggt attattttag
    10381 cgataggacg tatcattggt gagtcagcag cacttatttt cacagctgga acagttgcaa
    10441 aagtagtaca ttcggtattt agttcatctc gtactttagc agtacatatg tatgctattt
    10501 cgggtgaggg cttatatgtt gaccaaactt atgcgactgc ggtcatctta ttgcttttag
    10561 ttattattgt caattttgta tctggactag tagccaaacg attaggaagt gagtcgaaca
    10621 gtgagtaaat tagtaattaa taatttagat ttgtattatg gagaatttca cgctctgaag
    10681 gatgttaatt tagacattga agaaaaagaa atcacagcct ttattggacc ttctgggtgc
    10741 ggaaagtcta ctctcttgaa aagtattaac cgtatgaatg atttggtcaa aaattgcaaa
    10801 atcacagggg atattacttt agaaggcgag gatgtctata gacaattgga tattaaccaa
    10861 ttgcgtaaaa aggttggaat ggtgtttcaa aaacctaatc cgtttccaat gagcatttat
    10921 gataatgttg cttttggtcc aagaacgcac ggtattcatt ccaaagcaga gttagatgat
    10981 atcgttgaac gttcccttaa acaggcggct ctatgggatg aggttaaaga tcgtcttcac
    11041 aaatctgctc tgggtatgtc tggcggacag cagcaacgtt tgtgtattgc gcgtgctctt
    11101 gctattgagc ctgatgtttt acttatggat gaacccacaa gtgccttgga tcctatttca
    11161 acagcaaaaa ttgaagaatt ggttatccaa ctgaagaaga actacactat tgttatagtg
    11221 acacataata tgcaacaagc tgttcgtatt tctgataaaa cagcattctt tctgatggga
    11281 gaagtggttg aatataacaa aacgagccaa ctcttttcat tgccacaaga tgagcgtaca
    11341 gaaaactaca ttacagggag attcggatga ttcgttcacg atttgcaagt caacttaatg
    11401 accttaataa agaaattatt tttatggggg cattgtgtga ggacatcatt gggaagtcct
    11461 taggtgctct aacaaatagt aatgatgttt accttgacga tatttctgaa acttatcaca
    11521 agattgaaca aatggaacgg gatattgagg agagatgttt aaaactttta cttcgtcagc
    11581 aaccagttgc caaggattta cgccgtattt cttctgcatt aaaaatggtt tatgatatga
    11641 aacgtattgg tgctcaagct tatgaaattg cggaaattgt tagtttaggt catattatcc
    11701 aaggtgcagg ttctgaaagg gatcaactta acagcatgtc taataatgtc attagtatgc
    11761 tgactaagag tatagatgct tttatttatg acaatgaaga acaagcgcat caagtaattg
    11821 aacaagatag gacagttaac caggaatttg atacaataaa gaaacagtta gtgttatatt
    11881 tttcggtaca ggatgttgat ggtgaatatc ccatagatgt tttaatgatc gccaagtacc
    11941 tagagcgtat tggtgatcat acagtaaata ttgctaaatg ggtgcttttc tcaataacag
    12001 gtaacctaga tggagaagcg taatgatata ttgtgttgaa gatgatgctg atattcgcga
    12061 aatgatgctc tatactttgc aaatggcagg ttttacagct caaggattct cgagttcaga
    12121 gcttttttgg gaagctgttc aggagaaagt tccagacttg attttacttg atattatgtt
    12181 gccgggagat gatggtttga ctattttaga gcgtttaagg agaaaacatc aaacggaaat
    12241 gataccagta atcatgacaa ctgctaaagg tagtgagtat gataaagtta aagggttaga
    12301 tcttggagca gatgattatc tcgtcaaacc ttttgggatg atggaaatga tttcacgaat
    12361 aaaagcagtc ttgagacgta gccgccaagt agattcaaaa gctcatatta tcattggaaa
    12421 tttagagatt gacccgacta attattgggt caaaagggga acggaaaaaa tccacttaac
    12481 cctaaaggaa tttgagttat tagtattatt cttccgtaat cccaatagag tctttacaag
    12541 gcaagaactt ctggataagg tttggggaga acaattttta ggagaaacta gaactgtgga
    12601 tgttcacatt gggacactgc gaacaaaact tggtgaggat ggctatttga tcgctacagt
    12661 aaggggagtt ggataccgtt tggaggaaag acatgactaa aaaaattttc cgtacaactt
    12721 tatccgctag tttgggaatt gttttagtta caattttgat gattatggga tttctctata
    12781 attacttcaa tcgcattcag cgagaacagt tgagaacaca aacggcactg gcgtcacaag
    12841 gaatttcctt tgaaggtaaa gattattttg aaaatcttaa aacttcaaat gtgcgtatta
    12901 cttgggtaga taataaggga caagtgcttt atgataccca atcagatgca aaacatatga
    12961 aaaatcacgc caatcgtcaa gaaattaaag aagctatcaa gagtggttat ggtgagagca
    13021 cgaggtggtc agcgacactt actgagaagt ctatctatgc tgcgcaacgt ttaaataatg
    13081 gaacgatagt tcgtttatca gttgcgcaac aaactatttt ttatcttctc cttggtatga
    13141 tgagtccctt agcaattatc atcttacttg ctattatttt atctgtatta attgcacgat
    13201 atatagcaaa aaaagtatca gagcctttaa acaatattga cttagatcat cctttatcaa
    13261 atgatagtta cgaggagata acaccattat taaggcgtct tgatagccat caggctaaga
    13321 ttcaacatca aaaactgtta ttacaaaagc gtcaaaaaga atttgatacc attatttcta
    13381 aaattaagga aggaatgatt cttttagatg atcaagcaag aatcgttagt atcaacgcag
    13441 aagctttaaa gttattccaa attaatgatg attggcatgg gcgctttatg atggaagtta
    13501 gccgagattt aactctcaaa gacttaattg atcaaggact aaaagggaaa aagaaagagg
    13561 ctaatattga cattgaaaat aatcattacc gcgtcttggt aagaccaact acagataaca
    13621 accgagtaac aggattggtt gttctactgt ttgatgtgac tgaccaactc caaatggaac
    13681 aacttcaacg tgaatttact gccaatgtct ctcatgaatt gaaaaccccg cttcatgtta
    13741 tttctggtta ctcagaactg ttagctaatc aaatggttcc aaacgaagaa gtcccacaat
    13801 ttgcagctaa aattcataaa gaatctgaac gattagttaa attggttgag gatattatca
    13861 acctttctca ccttgatgaa caagaaaaat taccgcaaga gacagtaaat ctgtatgact
    13921 taacacaaaa agttttagaa ggtttacaag ctaaagcaga taagaagcac atacaaatca
    13981 attttaatgg agaagaagct attttgcgag ggaatccagt tttattgaat tccttggtgt
    14041 acaacttgtg tgacaatgct atcacctata atcatgagaa aggtcaagta aatgtgactt
    14101 tgaaaaattc acctgataca attaccttag aagttagtga tacgggacta ggcattgcag
    14161 agaaggacaa aaaacgtatt ttcgaacgtt tctatcgcgt cgataaaagt cgctcaaaaa
    14221 tagttggagg gactggttta ggtttgtcta ttgtaaagag tgctttagat tttcacaatg
    14281 gtagtataaa agtggacagc catttggggc agggaacaac aatgaccgtt ttacttcaca
    14341 aacaataaaa actcacaaat aaaagtctag atgatattat ctagactttt ttagtaattc
    14401 agaaaaaata tataagtaag ggtttacaaa aaacaaataa gtgctataat aaaaccataa
    14461 cgcaaacgtt tgcactatta agtgcataaa aggagattga agatgaaaaa taatgtcaaa
    14521 cagctcttta gttttgaatt ttggcagaag tttgggaagg ctttgatggt tgttattgct
    14581 gttatgccag cagcaggatt gatggttagt attggtaact ccattaggct attggatcca
    14641 tcaaatgttt tattagggcg catagctaat gttattgctc aaattggttg gggagttatt
    14701 ggtaacttac acatcttatt tgcattggct attggtggta gctgggctaa agaacgtgcc
    14761 ggtggtgctt ttgcggctgg cttatccttt attcttatta accttattac agggaacttt
    14821 tttggtgtga aaacagatat gttagcaaac agtaaagcaa ctgttcaaac ggtatttggt
    14881 gcgaccatca gagtttcaga ttactttgtt aacgttcttg gacaacctgc tcttaatatg
    14941 ggtgtatttg tcggtattat ttcaggtttc gttggtgcaa ctgcctttaa taaatattat
    15001 aactatcgta aattaccaga tgctttaact tttttcaatg gaaaacgttt tgttccattt
    15061 gttgttattt atcgttcagt cattgttgca ttaattttat cagtattttg gccggttgtt
    15121 caatcaggta tcaatggctt tggtaagtgg attgcaagtt cacaagacag tgctccaata
    15181 ttagcaccat ttgtctacgg tacgttagag cgtttattgc tcccatttgg tttacaccat
    15241 atgttaacta ttccaatgaa ctacacgcaa ctaggtggca catacactgt ccttacaggt
    15301 gcaactaaag gagcacaggt gttgggacaa gatccactgt ggttagcatg ggttggtgat
    15361 ttaatcaact taaaaggctc aaattcaagt caataccatc acttattgac aagtgtcact
    15421 ccagcacgtt ttaaagtggg gcaaatgatt ggtgcttcag gtattttgat gggattatct
    15481 tatgctatgt atcgtaacgt tgataaagat aaaaaattga aatacaagtc aatgtttatt
    15541 tcagcagcag cggctacttt cttgacaggt gttactgagc caattgagta catgttcatg
    15601 tttgcagcaa tgccactcta ccttgtatat gctgtcgttc aaggatgtgc gtttgctatg
    15661 gcagatattg ttaacctccg cgttcattca tttggtaata tagaattttt aacacgtgtt
    15721 cccatgggaa taaaagcggg ccttggtggt gatattttca actttgtttg ggtgacactt
    15781 ctttttgcag tattgatgta ctttattgca aactttatga ttaaaaaatt caatttagca
    15841 acagcaggac gtaatggtaa ttatgataac gaagaggttg ataatgctcc ttcaacagct
    15901 tctggttctg ctgatgcaaa ttctcaagtt gtacaagtta ttaatttact tggtggtcgt
    15961 gataacattg aagatgtaga tgcatgtatg actcgtcttc gtgtcacagt taaagatggg
    16021 aatagcgtgg gttctgaagc agcttggaaa aaagctggtg caatgggatt ggtgttaaaa
    16081 ggtaatggcg ttcaagctat ttacggacct aaagcagatg tccttaaatc agacattcaa
    16141 gatttgctag attcaggtac agtgattcca atagttgatt tagaaacagg ccagcctgta
    16201 gcagcaaccc aagttacaac ttataaagga ataacagaag aaattgtttc ggtggctaat
    16261 ggtcaagtag aagcattgga tgttgttaaa gaccctgtct tttctcaaaa aatgatgggt
    16321 gatggctttg ctgtggaacc aactgatggc aatatctatg ttccagtttc aggtacagtt
    16381 actagtgtct ttccaacaaa acatgctttt ggtttattaa cagaaagtgg cctagaagtt
    16441 ttagtacata tcggtctaga tacagtagct cttgatggtc aaccatttga ggttaaaatt
    16501 tctagtggtc aaaaagtggt agcaggcgat cttgctgtag tagcagattt ggaagctatc
    16561 aaagcagctg gtaaagagac aagtgtgatt atcgtcttta caaatgtttc agatataaaa
    16621 actgttaaac ttgaaaaatc aggtccacaa atagctaaaa cagttgtggc aaaagtcgaa
    16681 ctgtaacaaa cgataggagc tgggcaagtc tcagcttctt tctgatgaaa ggatactcta
    16741 tggcaaaatg cttaacgtta aatactcatt catggatgga agtaaatgct ctaaagaaat
    16801 tatttgattt agcagagcat atcttccgtg aaaaatatga tatcatctgc ctacaagagg
    16861 tgaaccagag catatcttcc cctcttgcta cgagtagtcc aaattatcat ccgattgaag
    16921 gtacaccagc tcttcatcaa gataactttg ccttgcaatt agtgcattac ctaaatcttc
    16981 aaggtctaca ctatcattgg acatgggctt ataatcatat tggttatagt aagtaccatg
    17041 aaggggtagc gatactttct ctgaaaccat taaagccaga tgatatttta gtttcagctg
    17101 tagacgatga gactgattat catactcgtc gtgctctggt agctgagacc actctgaatg
    17161 acaaagtcgt tacagtagtt agcctccatt tatcatggtt tgagaaagga tttgcggagg
    17221 agtggaaacg actagaaact accctacttg aggtagaaac tccactttta ctcatgggtg
    17281 actttaataa tcctactgga aatcagggtt atgaacttgt tcttaatagt ccattagcat
    17341 taaaggatag ccaccaaata gccaatcatg tatttggaga ccatactatt atggctgata
    17401 ttgatggttg ggaagggaat aaaaaggctc ttaaagttga ccatattttt actagcgaag
    17461 acctttcaat cagttcaagt caagtagttt ttgaaggtgg tgaagcacct gtcgttagtg
    17521 atcattacgg cttggaaatt acgttaaatc tttagattaa tctatttaat atattaaaag
    17581 catcctaaat ttgtctataa aacaaaactt agggtgcttt ttgaaaaata tgtcacatta
    17641 tttaacttct tacgaagttt ggaatgcatg agaaaatatg ataaaat
//
LOCUS       DATROY010000027        17565 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.027, whole genome shotgun sequence.
ACCESSION   DATROY010000027 DATROY010000000
VERSION     DATROY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17565
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(23..1804)
                     /locus_tag="VBD11_000779"
     CDS             complement(23..1804)
                     /locus_tag="VBD11_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6953747.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGYFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1974..2576
                     /locus_tag="VBD11_000780"
     CDS             1974..2576
                     /locus_tag="VBD11_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6953748.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2623..4029
                     /gene="pepV"
                     /locus_tag="VBD11_000781"
     CDS             2623..4029
                     /gene="pepV"
                     /locus_tag="VBD11_000781"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6953749.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4126..4710
                     /locus_tag="VBD11_000782"
     CDS             4126..4710
                     /locus_tag="VBD11_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6953750.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4725..5459
                     /locus_tag="VBD11_000783"
     CDS             4725..5459
                     /locus_tag="VBD11_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953751.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5628..9386
                     /locus_tag="VBD11_000784"
     CDS             5628..9386
                     /locus_tag="VBD11_000784"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO6953752.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9521..10439
                     /locus_tag="VBD11_000785"
                     /pseudo
     CDS             9521..10439
                     /locus_tag="VBD11_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
     gene            complement(10481..10657)
                     /locus_tag="VBD11_000786"
     CDS             complement(10481..10657)
                     /locus_tag="VBD11_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6953753.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10768..11658
                     /gene="miaA"
                     /locus_tag="VBD11_000787"
     CDS             10768..11658
                     /gene="miaA"
                     /locus_tag="VBD11_000787"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6953754.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11749..12987
                     /gene="hflX"
                     /locus_tag="VBD11_000788"
     CDS             11749..12987
                     /gene="hflX"
                     /locus_tag="VBD11_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6953755.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12980..13627
                     /locus_tag="VBD11_000789"
     CDS             12980..13627
                     /locus_tag="VBD11_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6953756.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            13669..14598
                     /locus_tag="VBD11_000790"
     CDS             13669..14598
                     /locus_tag="VBD11_000790"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6953757.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            14600..15361
                     /locus_tag="VBD11_000791"
     CDS             14600..15361
                     /locus_tag="VBD11_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6953758.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            15358..17556
                     /gene="recJ"
                     /locus_tag="VBD11_000792"
     CDS             15358..17556
                     /gene="recJ"
                     /locus_tag="VBD11_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6953759.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIYDFMMS"
BASE COUNT         5953 a         2669 c         3421 g         5522 t
ORIGIN      
        1 tctactaagc tttttattta atttaaccct cgctatcaat aagttggaga gctctttgat
       61 aataattagg gctatttttg atatctttat taatgacctt gaaaagattg agtttatctg
      121 tgatgattcc attaactggg ctttgtaaat aacgtatcat aaggttatca cggttaatag
      181 tccaaacata gagctgttta ttatattctt gggcgagata tgcaatccaa tcattgtatg
      241 aaaattcctc aatcccataa aagtcaacgt tatgattttg aagcgttccc caatgtagtg
      301 ggattaggta acctgtatca agttgtggca attttttctc aaccttttca atgagtgtca
      361 agttgagaga cattacttta tgcttggttg atagacgaag ctctttaaat tctttgataa
      421 attcatccgc atagatatcg aggttttgat tatacggttt caattcaatc atgatttttt
      481 gatctagtga ctgagcggtc ttcataaatt ctgccaatga tggaatgtaa ctagcaaaat
      541 acccttggtg aattgggatt tttgtaactt gatctaaagt tagtttagaa acatctttgt
      601 tcataccaga gagtcgtttg agattatcat catgaatgac tactaattta tgatcttttg
      661 tcatgataac atccatctct gaataatctg cttttgcttt atgggctgct tttaatgctt
      721 caatagaatt ttcaacgcca tgtgatgtat cactacgatg ggctattaat tcttttttaa
      781 ttgaaatgtt atcttgtaag agagccagag ctatagcatt ttttaaggca gtggtaccaa
      841 tagctagagc tagaataaca caagcaatgt gtttaaattt ttgcttatgg tggacgcctt
      901 tccaagctct ccattgttga acaatacttt tattttgttt aatatctgct aataggtgac
      961 ttatgattaa acatttaata agaagtgtta cggcaaaaag tattgatttt aaaaggttat
     1021 agaaagtggc ttgaagagga aaattgtttc cagaatgatc aatgaatgaa aatgtaccag
     1081 caacaacagc tagtgcaact gttccgatta cagatataaa taaactggtt aaaataattt
     1141 tacttagaag acgccactga cgctttccgc ggctaagttt ccaactctct aaaagtgctt
     1201 ctttagcatt gtagtcagtt aaaatggtca gcgggagaaa ataaattaat cgaaggttta
     1261 agtaacaaat aagcagtaaa agggtagagt aacctatctt tccaaagact gtctttgtta
     1321 actcgccaga aataaagtca ggaattttaa gatgctctaa tatacttgac cttagaccaa
     1381 ggttagctag aggaattgtc aaaagtaagt agattgatac ccaaattagc tgtggtccta
     1441 aaagtgattt tattttttta ggaaagattg ttagccattt tttgatactg agagaaaatt
     1501 cagttttcag tatggcaata gtatagatag cgaactcagt atagatgaac ccaattagga
     1561 ttacgatata aagtataaag agtaataaag ctaagggatg attaaaaagc aaagtccaat
     1621 tgtctttagt cactgcggga gtatttgata atatcaatat ccgacgaaag ataaaactca
     1681 gaattccttt tactaaataa gcaactaaag tttggattaa gataccagtt ccaatgtaaa
     1741 cccaaaattg attttttaaa gaggttaagg ttagaaaaaa atcttctttt aatgctcgta
     1801 gcatgaatag ctcctttttt atagtcatta gatattgtac cacaagtttg tttgtttttt
     1861 atggtagttt tcaaaccggt ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc
     1921 attttaaaaa gtgatagaat agtagggaaa atcaaataga ataggaggtt tttatgaaat
     1981 ttctagaatt aaataagaag cgccatgctg tgaaacattt taatgataag ccagtcgact
     2041 ttaaagatgt tagaactgct attgaaattg cgacattagc gccatctgct aataatattc
     2101 agccttggaa gtttgttgtt gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg
     2161 aaagtaattg taaccaaatt aatcaagcgc agtatgttat tgctttattt acagatacag
     2221 atttaggaca gagatcacgg aaaatagctc gtattggtcg tagatctctt cctgatgatt
     2281 taattggtta ttatatggaa actctaccac cacgctatgc tttatatagt gaaaaacaaa
     2341 ctggagagta tctctcttta aatgcaggta tcgttgctat gaatttagta cttgccttga
     2401 cagatcaagg tattagttct aatatgattt tagggttcga taaagcgatt accaatgatg
     2461 tcttagagat agataaacgt tttagaccag aaatattaat tactgttggt tatagtgatg
     2521 aaaaggttga gcctagttat cgtttgccag ttgatcatat tattgaaaaa cgttaatttt
     2581 taagcacaaa ctacataata ttttatagaa ggagttgccg taatgacaat tgattttaga
     2641 gcagaagtag acaaacgcaa agacgctttg atggacgatt taataaatct tcttcgtatt
     2701 aactcggagc gtgatgattc acaagctgat gcagagcatc catttggtcc tggacctgta
     2761 aaagccttgg aatttttcct tgagatggct gagcgagatg gctacgaaac aaaaaacgta
     2821 gataattatg cgggacactt tacatttggt caaggcgaag aagaactagg tatttttggg
     2881 catttagatg ttgttcctgc aggcagcggt tgggatacag acccttatga accagttatc
     2941 aaggataacc gtttatatgc tcgagggtca tcagacgata agggaccaac aatggcatgt
     3001 tattatgctc taaaaattat taaagagctt gggctaccaa catctaagaa agttcgtttt
     3061 gttgtaggaa ctgatgaaga atctggatgg ggagatatgg attactactt cgaacatgtt
     3121 ggtcttccaa aacctgattt tggtttctct ccagatgcag aatttccaat tataaatggt
     3181 gaaaaaggta atatcactga atatttacat ttttcaggag aaaataaggg agctgttaga
     3241 ctgcatagct tttctggcgg acttcgcgaa aacatggttc cagaatcagc cacagcacgt
     3301 ttcacaagtc atttagatca gacaacactt ggagctagtc tagctgattt tgcaggtaaa
     3361 cataatttga aagcagaatt atctgtagaa gatgggcagt atatagctac agtttatggt
     3421 aaatctgctc atggttcaat accacaagaa ggtgttaatg gggcgactta tttagctctt
     3481 tatctaagtc aatttgattt tgaaggtcct gctcgtgctt tcttagatgt cacagccaac
     3541 attattcacg aagacttctc aggtgaaaaa cttggagtag cttatgaaga tgactgtatg
     3601 ggatcgttga gcatgaatgc aggtgtcttc cagtttgatg aaactaatga tgataatact
     3661 atcgctctta atttccgtta cccacaaggg acagatgcta aaactatcca aactaagctt
     3721 gagaaactta acggagttga aaaagtgact ctttctgacc atgaacacac accacactat
     3781 gtacctatgg acgatgaatt agtatcaacc ttactagctg tctatgaaaa gcaaactggt
     3841 cttaaaggac atgaacaggt tattggtggg gggacatttg gtcgcttact tgaacggggt
     3901 gttgcatacg gtgccatgtt cccaggagat gaaaacacta tgcatcaagc taatgagtac
     3961 atgcctttag aaaatatttt ccgttcggct gctatctacg cagaagctat ctatgaatta
     4021 atcaaataaa ataatcctta aactaaatat gtgatcaatg ataaagggtg gtgaagacat
     4081 gaaagtgtct ttgcctcttt tcataaggtt agatttggag actttatgac tgacttggaa
     4141 aaaattatta aagcaataaa aagtgattca cagaatcaaa attatacaga aaatggtatt
     4201 gatcctttgt ttgctgctcc taaaacagct aggatcaata ttgttggaca agcacctggt
     4261 ttaaaaactc aagaagcaat actctattgg aaagataaat ctggagatcg tctacgccag
     4321 tggcttggag ttgatgaaga gacattttac cattctggaa aatttgctgt tttgccttta
     4381 gatttttatt acccaggcaa aggaaaatca ggagatttac cccctagaaa aggttttgcg
     4441 gagaaatggc accctcttat tttaaaagaa atgcctaatg ttcaattgac cttgctagtt
     4501 ggtcaatatg ctcagaaata ttatcttgga agctccgcac ataaaaatct aacagaaaca
     4561 gttaaatctt acaaagacta tctacccgat tatttacccc tggttcaccc atcaccgcga
     4621 aatcaaattt ggctaaagaa gaatccatgg tttgaaaaag atctaatcgt tgatttacaa
     4681 aagatagtag cagatatttt aaaagattaa ggataggagt tagtatgaga gataatcatc
     4741 tacacacgca tttttcctat gattgtcaaa cggcatttga ggactatatt aatggtttta
     4801 caggtgaatt tatcacaaca gaacattttg atttatcaaa tccttacacc ggtcaagacg
     4861 atgttcctga ttatagtgct tattgtcaaa aaatagatta tcttaatcag aaatatggaa
     4921 atcgatttaa aaaaggaatt gaaatcggtt attttaaaga tagggaatca gatattttag
     4981 attatttaaa aaataaagaa tttgatttaa aactattgtc aatccatcat aatggtaggt
     5041 atgattatct gcaagaagaa gctctgaaag taccaacaaa ggagctttta gcagattact
     5101 ttaatcgtat ggaatttgcc ataggccgtg tggaagcgca cgttttagat cactttgatt
     5161 atggttttcg taagttaaac ttagatgtag aagatttaaa accgtttgaa acgcaattga
     5221 agcgcatttt cagaaagatg ttatctaagg ggttagcttt tgaactaaat accaaatccc
     5281 tttatctata tgggaatgaa aaactttatc gctatgcttt agagatacta aaacagcttg
     5341 gttgtaaaca atactctata ggctctgacg gtcatattcc tgaacatttt tgttatgaat
     5401 ttgatagact tcaatgtctg ctaaaggact atcaaattga tgaaaatcat ttgatatgag
     5461 gaaatttttg ataaaaaagc taggcaatat tgcttagctt ttttgtaatg tcattgatag
     5521 ttttagtgaa aatttcaaaa aaataaagaa atcacttact tgttgcaagc gcttgcgtaa
     5581 attgttatga ttttattggt aacaattcat taaaaaagga gaatgatatg aaaagaaaag
     5641 acttatttgg tgataaacaa actcaataca cgattagaaa gttaagtgtt ggagtagctt
     5701 cagttgcaac aggggtatgt atttttcttc atagtccaca ggtatttgct gaagaagtaa
     5761 gtgtttctcc tgcaactaca gcgattgcaa agtcgaatat taatcaggtt gacaaccgac
     5821 aatctactaa tttaaaagat gacataaact caaactctga gacggttgtg acaccctcag
     5881 atatgccgga taccaagcaa ttagtatcag atgaaactga cactcaaaaa ggagtgacag
     5941 agccggataa ggcgacaagc ctgcttgaag aaaataaagg tcctgtttca gataaaaata
     6001 ccttagattt aaaagtggca ccatctacat tgcaaaatac tcccgacaaa acttctcaag
     6061 ctataggtgc tccaagtccg accttgaaag ttgctaatca agctccacag attgaaaatg
     6121 gttactttag gttacatctt aaagaattgc ctcaaggtca tcctgtagaa agcactgggc
     6181 tttggatatg gggagatgtt gatcaaccgt ctagtaattg gccaaatggt gctatcccta
     6241 tgactaatgc taagaaagat gattacggtt attatgttga ttttaaatta tctgaaaaac
     6301 aacgaaaaca aatatctttt ttaattaata acaaagcagg aacaaattta agcggcgatc
     6361 atcatattcc attattacga cctgagatga accaagtttg gattgatgaa aagtacggta
     6421 tacatactta tcagcccctc aaagaagggt atgtccgtat taactatttg agttcatctg
     6481 gtaactatga ccacttatca gcatggctct ttaaagatgt tgcaaccccc tcaacaactt
     6541 ggccagatgg tagtaatttt gtgaatcaag gactatatgg aaggtatatt gatgtaccac
     6601 tgaaaactaa tgccaaagag attggttttc taatcttaga tgaaagtaag acaggagatg
     6661 cagtgaaagt tcaacccaac gactatgttt ttagagattt agctaaccat aaccaaattt
     6721 ttgtaaaaga taaggatcca aaggtttata ataatcctta ttacattgat caagtgcagc
     6781 taaaggatgc tcaacaaact gatttaacaa gtattcaagc aagttttaca actctagatg
     6841 gggtagataa aactgaaatt ttaaaagaat tgaaagtgac agataaaaat caaaatgcta
     6901 tacaaatttc tgatatcact ctcgatacta gtaaatctct tttaataatc aaaggcgact
     6961 ttaatcctaa acaaggtcat ttcaatatat cttataatgg taacaatgtc acgacaaggc
     7021 aatcttggga atttaaagac caactttatg cttatagtgg aaatttaggt gcagttctca
     7081 atcaagatgg ttcaaaagtt gaagccagcc tctggtcacc gagtgctgat agtgtcacta
     7141 tgattattta tgacaaagat aatcaaaaca gggttgtagc gactaccccc cttgtgaaaa
     7201 ataataaagg tgtttggcag acgatacttg atactaaatt aggtattaaa aactatactg
     7261 gttactatta tctttacgaa ataaaaagag gtaaggataa ggttaagatt ttagatcctt
     7321 atgcaaagtc attagcagag tgggatagta atactgttaa tgacgatata aaaacggcta
     7381 aagcagcttt tgtaaatcca agtcaacttg gacctcaaaa tttaagtttt gctaaaattg
     7441 ctaattttaa aggaagacaa gatgctgtta tatacgaagc acatgtaaga gacttcactt
     7501 ctgatcaatc tttggacgga aaattaaaaa atcaacttgg tacctttgca gccttttcag
     7561 agaaactaga ttatttacag aaattaggag ttacacacat tcagctttta ccggtattga
     7621 gttattttta tgttaatgaa atggataagt cacgctcaac agcttacact tcctcagaca
     7681 ataattacaa ttggggctat gacccacaga gctattttgc tctttctgga atgtattcag
     7741 agaaaccaaa agatccatca gcacgtatcg ccgaattaaa acaattaata catgatattc
     7801 ataaacgtgg catgggggtt atacttgatg tcgtctataa tcacactgca aaaacttatc
     7861 tctttgagga tatagaacct aattattatc actttatgaa tgaagatggt tcaccaagag
     7921 aaagttttgg agggggacgt ttaggaacca ctcatgcaat gagtcgtcgt gttttggttg
     7981 attccattaa atatcttaca agtgaattta aagttgatgg tttccgtttt gatatgatgg
     8041 gagatcatga tgcggctgcg attgaattag cttataaaga agctaaagct attaatccta
     8101 atatgattat gattggtgag ggctggagaa cattccaagg cgatcaaggt aagccggtta
     8161 aaccagctga ccaagattgg atgaagtcaa ccgatacagt tggcgtcttt tcagatgata
     8221 ttcgtaatag cttgaaatct ggttttccaa atgaaggtac tccagctttc atcacaggtg
     8281 gcccacaatc tttacaaggt atttttaaaa atatcaaagc acaacctggg aattttgaag
     8341 cagattcgcc aggagatgtg gtgcagtata ttgctgcaca tgataacctt accttgcatg
     8401 atgtgattgc aaaatcaatt aataaagacc ctaaggtagc tgaagaagat attcatagac
     8461 gtctgcgttt aggaaatgta atgattttaa catctcaagg gacagcattc attcattctg
     8521 gtcaagagta tggtcgtacg aagcgtttac ttaaccctga ttacatgaca aaagtttcag
     8581 atgacaaatt gcctaataaa gcaacactta ttgaagctgt taaagaatac ccatatttta
     8641 ttcatgattc atatgattct tcagatgcca ttaatcattt tgattgggca gcagccacag
     8701 ataataacaa acacccaatt tcaacgaaaa cacaggccta tacagcaggt ttaatcacat
     8761 taaggcgttc aacagatgct ttccggaaat tgagcaaagc agaaattgat cgtgaggtta
     8821 gcttgattac agaggtaggt caaggtgata ttaaagaaaa agatttggtt attgcttacc
     8881 aaacaataga ttctaaaggc gatatttacg cagtatttgt taatgctgat agtaaagcta
     8941 gaaacgtttt actaggtgaa aaatataaac accttttaaa agggcaagta attgttgatg
     9001 ctgatcaagc ggggattaaa ccaatctcaa ctcctagagg tgttcatttt gaaaaagata
     9061 gtttgctgat tgatccatta acagcaattg tgattaaagt tggcaaagtt gctcctagcc
     9121 ctaaggagga attgcaagca gattatccca aaacacaatc tttcaaggga tctaaaacgg
     9181 tagaaaaagt aaatagaata gctaataaga cctcaataac tcctgtagtt tctaataaga
     9241 ccgattcata tctgacaaat gaagctaatt tgccaaaaac tggagataag tcatcaaaaa
     9301 tactaagtgt agtaggaata agcattctag caagtctact tgctctacta ggtctctctt
     9361 taaagaggaa tcgtcactta aaataaaagc aaactttaga agagcttaaa aaagaagtta
     9421 gtcaatgggc tagcttcttt tcattaagtt aataaaatca cttatcagca aaatctaatt
     9481 gtgttataat ggatgtaaac gtttttttat gaggtatatg atgaaaaatt gggaaaaacc
     9541 accattagtg atggcgggat tagtattggg gctattagct ttggggaacc ttctagaggg
     9601 atatggcacc tattttcgct attgtttagg gctagtagca cttgtttttt ggattttttt
     9661 aatcaaagga atattaaaaa ataaaaaaga aagcaggaag gagttaagca atcctttgat
     9721 tgcttctgtc tttactactt tttttatggc aggtatgatt ttatcgacct atatcttatt
     9781 atttagaagt ttaggaatat gggtagcagt tctgtcaaaa aggtgtatgg tggttgtcat
     9841 ttattgcgtt aatcattcat atggctattt tttcatggaa atatttgaga cacttttcga
     9901 tggctaatct ttttccttct tggtctgtcc tctatgttgg tataggggta gctagcctga
     9961 cagcaccaat tagtggacaa ttcactattg ggaaaatagt tttttggtat ggttttattg
    10021 caactttagt attattacca tttctattca ttaaagcata taaaatcggg ctaccttcgg
    10081 cagttaagcc taatataact actatttgcg ctccaatgtc gctaatcaca gcgggatatg
    10141 ttaatagttt tgtgtcacca aatagaggtt tattattgct attaatcgta atggcacaat
    10201 ttctttattt tttcattctt tttcaggtgc ctaaattact gattggtgat tttacaccag
    10261 gtttctcagc ctttactttc ccccttgtta tctcagctac ctcacttaaa ttaagtattc
    10321 agcatctatc attaccagtt gatatacagg ggctagtcca ctttgaaatt gggacaacaa
    10381 ctcttattgt tatgatagta atggtacgtt atattttctt tttaaggcga acaatttaat
    10441 ataaaataac tcccttacta gctagtaagg gagttattgt ttaatgagag acacgtttac
    10501 gtgcagcttt tttacgattt tcttcgataa aagctatttt ttcttcctca ggttcaataa
    10561 ttgttttttt gatagcaaat gcagcacctg caagagtagc agctgttgca acgactcctg
    10621 ttgctattcc tttagcaaat gcgaattttt taaccataat aattctccaa tctatgatga
    10681 agactctgct tctatctatg atataattat ggtaacgaaa tactagttaa atttcaagta
    10741 aaaaccttta tagaattgag aagtatcatg agaaaaatca agcttatagc agttgttggt
    10801 ccaactgcgg ttgggaagac agcattagga attgaattag ctaaaacttt caatggcgaa
    10861 atcattagtg gcgatagtca gcaagtttat cagaagttag atatcggaac ggctaaagct
    10921 agtaaagaag agcaagaaca agcttatcat cacttgattg atgttcgtga agtaaatgag
    10981 aattattcgg tttatgattt tgtcaaagag gcaaaagtag ctattgatac gattatttct
    11041 aaggggaaaa tccctattat tgttggaggt actggcctat atttacagag tttgtttgag
    11101 ggctatcatt taggaggcga agtcaatcaa gaaactctta aggcctatcg tgaaaaatta
    11161 gagtctctaa gtgatgaaga tttatttgaa aagttgacag aacaatccat tgttataccg
    11221 caagttaatc gtcgacgtgc catccgtgca ttagaattgg cgaagtttgg gaatgatttg
    11281 caaaatagtg aaagtccgta tgatgtttta ttaattggat tgaatgatga tcgtcaggtg
    11341 ctctatgatc gtatcaatag acgagttgat ttgatgatag acaatggtct cttagatgaa
    11401 gctaaatggc tatacgataa ctaccctagt gttcaagcaa gtagaggaat tggctataaa
    11461 gaattatttc cttacttttc aaagcaaata ccattagaag aagctgttga taaactaaaa
    11521 caaaatacca gacgttttgc aaaacgccaa ttaacatggt ttagaaaccg tatgaatgtc
    11581 gaatttatta tggttggtga ggaaaattat caacaaaaaa tcaagcgaaa agtcagtgat
    11641 tttttatcat caaagtaaat aaagccgttt ctcgcattta aagggcatta tgttataata
    11701 aagattacgt atttagaaca agaaaaaaat attgaaagga gattataggt gatagaaact
    11761 aaagaagagc aagaacgtgt tattttagtt ggggtagaac ttcaagatac tgagaatttt
    11821 gaaatgtcca tggaagaact tgctagctta gctaagacag caggagcaaa tgttgttaat
    11881 cattattatc aaaaaagaga taaatatgat agtaaatcat ttatcggatc tggtaaatta
    11941 gaggagataa aagctattgt agaagcagat gagattgata ccgttgtggt taataatcgt
    12001 ttgactccgc gacaaaactc taatttggaa gctgaacttg gtgttaaagt tattgaccgt
    12061 atgcaactta ttttagatat ctttgctatg cgtgctagaa gtcatgaagg aaaattacag
    12121 gttcatctag cgcaattgaa atatatgtta ccacgcttag ttggacaagg tattatgctt
    12181 agtcgtcaag caggaggaat cggaagccga ggtccaggag aatctcagtt agagttgaat
    12241 cgtcgttcca ttcgtcatca aatttctgat atcgaaagac aattgaagat tgtagaaaaa
    12301 aatcgcgaga cagttcgaga aagacgtgtt gattcgacaa cctttaaaat tggattaatt
    12361 ggatatacaa atgctggcaa gtcaactatt atgaatgttt tgaccgatga taagcaatat
    12421 gaagccaatg aattatttgc aacgttagac gctacaacca agcagatcta tttacaaaat
    12481 caatttcaag tcactttaac agatactgtt ggttttattc aagatttacc tacagagtta
    12541 gttgcggctt tcaagtctac cttagaagaa agtcgacatg ttgatttatt acttcatgtt
    12601 attgatgcta gtgaccctaa tcatgaagaa cacgaaaaag ttgttatgga gattcttaaa
    12661 gacttagata tgatagatat tcctcgttta gcgatataca acaagatgga tgtcactgag
    12721 cagctgaatg caacaacttt tcctaatgtg agaattgcag ccaaaaaaca agggagtagg
    12781 gatttattac gccgtcttat tgtggatgaa atacgacata tctttgacga attttcgatt
    12841 agagtgcatc aaaatcaagc atataagctc tatgatctca ataaaatagc attgcttgac
    12901 acctacacct ttgaagaaga atacgagaat attacaggct atatttctcc taaacaaaaa
    12961 tggaagctgg aagaatttta tgattgatta tattgatttg gctttgactt atggtggttt
    13021 tacgagctta gataaagttt atctagagaa aaaactagat ggcctctcta agcaacaacg
    13081 acttgatttt atcactccac ctcctagtgt gattaatgct tattttgctg aaatatatca
    13141 aaagcaaggg ccagaagcgg caactgatta ctattttgat ctttccaaag ccttgggctt
    13201 gtttccaaaa catttatcgt ttgatgaaga aaaacctttt attcgtttga atttatcagg
    13261 aaaatcattt ggttttgctt acctaaatga tcaagaagaa gcgagtgttt tttctgaagt
    13321 taaagaagtc attacaccgc agttactctt agaaattgca cagatttttc ctcaatataa
    13381 agtctatcaa gatcggtctg gtatcagaat ggcaaaaata gattttgacg aaacagaatc
    13441 tcaaaatatt acaccagaaa caagtttact agggaatgtc ttacaactta aaaaagatat
    13501 tattaaaata actagtttca atcaagaaga gctattagag ttggttaaaa caaagtcagg
    13561 tgaatactat tattctagtc aaggacgtga gtcagttatt tatattgata ctaaatctac
    13621 taattaaatt tattaagtta tggaaggatt ataaaaaagt caacaagtat ggaaattcaa
    13681 tttttaggaa ctggagctgg tcaaccagcg aaagctcgta atgtttcaag tttagtcttg
    13741 aaattattag atgaaattaa cgaggtttgg atgtttgatt gtggagaggg tacacagcgt
    13801 caaattttag aaaccacgat taaacctcgt aaagtcaaaa aaatttttat cacgcacatg
    13861 cacggagacc atgtttttgg tcttccaggt tttttatcca gtcgggcttt tcaagccaat
    13921 gaagaacaga cagatttaga tatttatgga ccggtcggta taaaaagttt tgttatgact
    13981 ggcttaagga catcaggttc acgtttacct tatcgaattc attttcacga gtttgatgaa
    14041 tcatcactcg gaaaaattat ggaaacagat aaatttaccg tttatgcaga aaagctagat
    14101 catactatct tttgtatggg atatcgtgtt gtgcaaaaag accttgaggg cactttagat
    14161 gctgaagcat tgaaactagc aggtgttcca tttggtcccc tttttgggaa agtcaaaaat
    14221 ggtgagaatg ttaccttaga agatggtaga gaaattattg ctaaagatta tatttctgaa
    14281 ccgaaaaagg gtaaagtaat cactattttg ggagatacta gaaaaacaga tgcaagtatt
    14341 aggttagctt tgggcgctga cgttttagtg cacgaatcta cttatggtaa gggtgatgag
    14401 cgaattgcta aatcgcatgg gcattcaacc aatatgcaag cagctgatat cgctaaacaa
    14461 gcaaatgcta aacgactatt attaaatcat gttagtgctc gttttatggg gcgagattgt
    14521 tggcaaatgg aagaagatgc aaagacgatt ttttcaaata cccatcttgt tagagatttg
    14581 gaagaagtag gtatataaaa tggcaagaac gatactgatt acaggagctt ctggtggttt
    14641 agcacaggca attattaacc aattacctca agacgaccat ttaattgtca cagggagatc
    14701 aagagaaaaa cttgaaaagc tttatggaaa gagaccaaac accttatgtt tatcactaga
    14761 cataactaat gacaatgctg taacgaatat gattgaaaaa atttatgttg aatttggtca
    14821 aattgatatt ttaattaata atgctggatt tggttctttt aaagaatttt gggattatag
    14881 tgatgaagaa gtcaaagaca tgtttgctgt gaatacattt gcaacgatgt ctatagctcg
    14941 tcagatagga cataagatgt ctcttgtaaa atctggtcat atcgttaata ttgcaagcat
    15001 ggctggcctc atagcaacaa gtaaagctag cgtttatggg gctagtaagt ttgctgtcgt
    15061 tggatttttg aatgctcttc gtttagaatt agcagaaaaa aatgtttatg tgacaagtgt
    15121 aaaccctgga ccaatcaaaa caggattttt tgctcaggct gatccaagtg gtgattattt
    15181 agcaagtata ggtcgttttg ttctaacacc agaaaaagta gctaaaaaag tagtttctat
    15241 tcttggaaaa aataagagag aattaaatct tccttttata ttagcttttg cgcataaata
    15301 ttatagttta tttccaaaaa cagctgatta ttttgccaga aaggtattca attataaatg
    15361 atatctgcta agtatagttg ggtcctcaat aaccaaaagc cagacgctgg cttttttgag
    15421 gcttcaaaaa aagaaaagat aagtgaagcg gtggcttcct taatttattc gcgaggtatt
    15481 aagacatcag cggaattaca tcatttttta caaactaatt tggaaaatct ccatgatcct
    15541 tatttgttaa atgatatgga taaagctgtt aatcgtatta gacgagctat tgaaaataat
    15601 gaaactattt tagtttatgg agactatgat gctgatggaa tgacatcagc atcaatcatg
    15661 aaagaagcat tagatatgat gggagctgaa gtccaggttt atctcccaaa tcgctttaca
    15721 gatggttacg gtcccaatca aagtgtttat aaatatttta ttgaacaaca agatgtttca
    15781 ttaatcatta cggttgataa tggtgtggca gggcatgaag caattgcata tgctcaaaat
    15841 caaggtgtgg acgtggtagt tactgatcat cacagtatgc cagcagattt accttgtgct
    15901 tatgctatta ttcacccaga acatcctgat gctaattatc catttcctta tttagcaggg
    15961 tgtggtgttg ctttcaaagt agcttgtgct ctcttagaaa caatcccgac agaaatgttg
    16021 gatttggttg ctattgggac gattgcagat atggttagcc taactgatga aaatcgtatc
    16081 atggtaaaag ctggacttga agttatgaaa gatagtgaaa gaataggctt acaggagcta
    16141 atatctttat caaatattga tttaaaaaca ctaaatgaag aaactattgg atttaaaatt
    16201 gctccacaat taaacgcttt aggaagactt gatgacccta atccagctat tgaattacta
    16261 acagggtttg atgatgaaga atctcaagca atagcccaaa tgattgatca aaaaaatgaa
    16321 gagcgcaaag aaatcgttca aactatcttt gaccaagcta tgcaaatgct tgatcaaaca
    16381 aagcctgtac aagttttagc aaaagaaaat tggcatccag gggttttagg tattgtggcg
    16441 ggtcgtattt tagagagaac tggacaacct gttattgttt tgaatattga agatggtatt
    16501 gctaaaggaa gtgccagaag tgtagaagca cttgatattt ttcaagcttt tgaccagcat
    16561 cgagaattat tcattgcttt tggaggtcac agcggagcag caggcatgac tttagaggaa
    16621 agtaaggtag gtgatctttc tcaagtttta tgtgactata tctctaaaaa acaattggac
    16681 atgagtcaga aaaaaactct gacaattgat agtgagctac gttttgacga acttagttta
    16741 gacactgtta gagattttga aaagttagcg ccatttggaa tggataataa aaaaccagtc
    16801 tttttgttaa aagattttaa agtgagtcaa gccagagtca tggggcaaaa cggtgctcat
    16861 ctgaaattaa aactagaaca agatggacaa gcacttgatt tggtagcatt taatatgggg
    16921 agtcaactac aagagtttca acaagcacaa catttggagt tagctgtcac tttatctgtt
    16981 aatcaatgga atggtgccac aaccctacaa ttaatgttgg aggatgcgcg tgtagatggt
    17041 attcaattat ttgatattag aagcaaagca agtagcttac ctcatggagt gccaatcctg
    17101 tctcaagaag agcagagcaa agaagttatt cttttgactg ttcccgatca tccacaagaa
    17161 ttaaaacaaa tgactcaagg taaacagttt gatgctatct attttaaaaa tgagatacca
    17221 aaaaattatt ttatatctgg ttatggtaca cgtgatcaat ttgctagttt atataaaaca
    17281 atatatcaat tcccagaatt tgacgttcgt tataagttaa aagagctcag ttcctacctt
    17341 catattcctg atattttatt aattaaaatg attcaaattt ttgaagaact acattttgtt
    17401 acgattacag aggggattat gacagtcaat aaagaagcag aaaagcgaga tatctctgag
    17461 agtcaaattt atcaagaatt aaaagagaca gttaagtttc aagaattaat ggctttaggg
    17521 acacctaaag aaatatatga ttttatgatg agttgataat atact
//
LOCUS       DATROY010000028        17470 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.028, whole genome shotgun sequence.
ACCESSION   DATROY010000028 DATROY010000000
VERSION     DATROY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17470
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(33..1106)
                     /locus_tag="VBD11_000793"
     CDS             complement(33..1106)
                     /locus_tag="VBD11_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6953760.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(1099..1875)
                     /locus_tag="VBD11_000794"
     CDS             complement(1099..1875)
                     /locus_tag="VBD11_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953761.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(1872..2666)
                     /locus_tag="VBD11_000795"
     CDS             complement(1872..2666)
                     /locus_tag="VBD11_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953762.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(2650..3804)
                     /locus_tag="VBD11_000796"
     CDS             complement(2650..3804)
                     /locus_tag="VBD11_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953763.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(3853..4755)
                     /gene="murB"
                     /locus_tag="VBD11_000797"
     CDS             complement(3853..4755)
                     /gene="murB"
                     /locus_tag="VBD11_000797"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6953764.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(4899..5387)
                     /gene="folK"
                     /locus_tag="VBD11_000798"
     CDS             complement(4899..5387)
                     /gene="folK"
                     /locus_tag="VBD11_000798"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6953765.1"
                     /translation="MTTVYLSLGSNISDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(5384..5746)
                     /gene="folB"
                     /locus_tag="VBD11_000799"
     CDS             complement(5384..5746)
                     /gene="folB"
                     /locus_tag="VBD11_000799"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6953766.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(5748..6551)
                     /gene="folP"
                     /locus_tag="VBD11_000800"
     CDS             complement(5748..6551)
                     /gene="folP"
                     /locus_tag="VBD11_000800"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6953767.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(6555..7118)
                     /gene="folE"
                     /locus_tag="VBD11_000801"
     CDS             complement(6555..7118)
                     /gene="folE"
                     /locus_tag="VBD11_000801"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6953768.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(7137..8399)
                     /locus_tag="VBD11_000802"
     CDS             complement(7137..8399)
                     /locus_tag="VBD11_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6953769.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQIPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(8401..9288)
                     /gene="rarD"
                     /locus_tag="VBD11_000803"
     CDS             complement(8401..9288)
                     /gene="rarD"
                     /locus_tag="VBD11_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6953770.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(9275..10141)
                     /locus_tag="VBD11_000804"
     CDS             complement(9275..10141)
                     /locus_tag="VBD11_000804"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6953771.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(10143..11426)
                     /locus_tag="VBD11_000805"
     CDS             complement(10143..11426)
                     /locus_tag="VBD11_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6953772.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            11603..12491
                     /locus_tag="VBD11_000806"
                     /pseudo
     CDS             11603..12491
                     /locus_tag="VBD11_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(12514..14061)
                     /locus_tag="VBD11_000807"
                     /pseudo
     CDS             complement(12514..14061)
                     /locus_tag="VBD11_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(14204..15580)
                     /locus_tag="VBD11_000808"
     CDS             complement(14204..15580)
                     /locus_tag="VBD11_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6953773.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHTLYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(15654..16661)
                     /locus_tag="VBD11_000809"
     CDS             complement(15654..16661)
                     /locus_tag="VBD11_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6953774.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(16769..17455)
                     /locus_tag="VBD11_000810"
     CDS             complement(16769..17455)
                     /locus_tag="VBD11_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953775.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         5864 a         3535 c         2642 g         5429 t
ORIGIN      
        1 ctctcatcag agaagtactt ttagttaata gattacttac gatacatttt aaattgtaag
       61 tacaaatcat tataaatacc aagccatttt tgacccaagt tattataaac ttctaaatga
      121 tcaattgttt tatcaggagg ataaaatgct ttatcatttt tgatatctgc tggtaacagc
      181 gcttttgctt tgagattagg ggtcgcataa ccaatatatt ctgcattttg tgccgcattt
      241 ttaggttcca tcatgaaatt aataaaggca taagcttctt tgcgatgctt tactgttttg
      301 gggattacaa tattatcaaa ccaaaggttc gatccttcac taggaacaac gtaatgcaaa
      361 tgcttgttcc catcaagcat ttctcgtgct tctccagaga atgtaacacc aatcgctgca
      421 tctccctgaa tcatatagcc tttcatctca tcagcaacaa tagccttaac gtttggtgtt
      481 agagcatcta actttttaga agcggctttt aattctgata tgttctttgt attcaatcca
      541 tatcccaagg aatttaaacc aactccaata acctcacgtg cactatcaac tagcatgatt
      601 ttatttcgaa actctggacg ccataaatca tcccaatgtt taggaggagt tttaactaat
      661 tgatcattat aaacaatccc aacagttccc caaaaataag gaatcgaata tttattttta
      721 ggatcgaaac tcaaattttt aaacctagct ccaatagcat cccaattagc tatctttgaa
      781 tgatcaagtt taaccagaag gttttctttt atcattttgt caatcatata atcactaggt
      841 acagcaatat catatgtcgt cccaccctgc ttaattttgg tgtgcatagc ctcattagag
      901 tcaaacgttt cgtattgtac ttcaattcca gtttcttttg taaatttttt taataaggct
      961 ggatcaatat agtctcccca gttataaata actaacttat ctgagttagg tgttgaacta
     1021 ctcttctttt ctaagtaagt ggttaaacca aagaggatca ggataacaag aataataccg
     1081 cctaaaaagg aataaactct acgcattctt ttctcccttc tctttcgaaa tatagtaata
     1141 accaattacc aataaaatac taaataaaaa gacaatagtt gacaaggcat taatttccaa
     1201 tgatataccg cgacgtgcgc gtgaatagat ttctactgaa agcgttgaaa agccattacc
     1261 tgtaacaaag aaagtaaccg caaaatcatc tagcgagtaa gtgaaagcca taaagaaacc
     1321 tgaaataata cccgacgaca aatagggtaa cataacttct ttaagcattt gccaagttga
     1381 agctcctaaa tcataggaag cattaatcat atcatcattc atttctttta aacgaggtaa
     1441 gaccattaaa acaacaatag gaatagagaa tgcaacatgg ctcaataata cagaagtgaa
     1501 acctaactgt aaaccaataa cagtaaatac taagagaaaa cttgcaccaa tcataacatc
     1561 tggtgctact aaaaggatat tattaagcga taaaatagag gtttgatgtc tacgtcttac
     1621 ctgataaatc caaatagctc caaaggtacc aataattgta gctaataagg cgctaagaaa
     1681 agccaaaaag aatgtttgca ctaaaattag cattaagcgg ctatcctgaa acagttctcc
     1741 atagtgacta aaggtgaatc cagtaaaaga attcatatcc ccacctttat tgaaagaata
     1801 aaaaatcaaa taaataatag gaatataaag gataataaaa acaaatgcta aatagatatt
     1861 tgcgaatttt ttcatgcttt cctctccttt gttaaccaca taatagcaac catcactaaa
     1921 attaaaataa cccctatggt tgagcccata cctttatttt gtgtgattaa aaaatgttgt
     1981 tctatggctg tccctaaagt aataactcga ttaccaccaa taagacgcgt aagcataaag
     2041 agacttaaac taggaataaa gacagattgt acacctgctc ttacaccatt cagagataga
     2101 ggaaatataa ccttctgaaa agtttgccat gcatttgcac ccaagtcatc acttgcataa
     2161 atgagatttt gatcaatatc atccaaggca ttaaaaatgg gtaataacat aaacggtaat
     2221 tcaatgtaag ctgctacaaa aagaaatgaa aaatctgtaa atagaatctg ttttgggccg
     2281 atacctataa aggttaagaa ggcgttaatt cccccttgtt gaccaaatat ccccataaaa
     2341 gcataggctt ttaacaaaag attaatccat gttggcaaaa taattagcat tagccataat
     2401 tgcttgtgtt ttaattttgt aagcaaataa gcagctggat agctaatcaa aagcgttatg
     2461 aaactaacga tgcctgcata taggaccgag ttaaagctca ttttaagata ggttcctgag
     2521 ctaaaaaacg tctggtaatt agctaaagta aagtgcccat taatatcaaa gaacgagttc
     2581 caagcaatca aagcaacagg tgctaataca aataagaata accaagccat ataaggaatt
     2641 gaaaatagac tagaggtttt cttcatttct ctcctcctcg atagcattga ttaaaccatc
     2701 ttcatgatcg tcagtgtcta catattcttc aatgcgcgca tcaaattctt cttctgtttc
     2761 attaagacgc ataatatgta tatcttctgg agtgaaatct aacccaataa cttcaccctc
     2821 aatagcttta cgagttgaat gaatcatcca ttcattacct aaatcatcat aagcaataat
     2881 ttcatagtgg acacctcgaa aaagttgtgt gtccacttta acttgaagtt ttccttcatc
     2941 gggtagggta atttgaagat cttctggacg aataactact tggactgatt cattagggcg
     3001 cataccgcca tcaacagcct caaagcgctt accgttaaat tcaaccaaat aatcctcaat
     3061 catcttgcca gataagatat ttgattcacc tataaaagtt gctacaaaat gattgatagg
     3121 ttcatcgtag atatctactg gtgttcctga ttgaacaatt tcaccttcat tcatgacgaa
     3181 aatccaatca ctcattgcca acgcttcttc ttgatcatgt gtgacaaaaa caaaggtaat
     3241 tcctaaacgt tgttgcagtt cgcgcaattc atattgcatc tctgtacgca atttcaaatc
     3301 caatgctgat aagggttcat ctagtaagac tactttaggt tgattgatga tagcacgcgc
     3361 tatcgcaaca cgctggcgtt gtcctcctga taatttttga atagctcgct tttcaaaccc
     3421 ttctaaacga accattttta gagtttcttg aacacgtttt tgaatttctt tcttatccat
     3481 ttttttcaac tttaatggaa aagcaacgtt ttcaaaaacc gtcatatgtg gaaacaaagc
     3541 atagttttgg aaaacggtat gaacatcccg tttattagtg ggaacatcat tgatgcgttt
     3601 cccatctaag taaatatcgc ctgtactcgc ctctagcaaa cctgcaataa tatttaagat
     3661 agttgattta ccacttcctg aggcccctaa taaggtgtaa aatttacctt cttcaagttc
     3721 aaaattaata tcttttaaga caaccgtgtt gctgtcttcg aatacctttg atacattttt
     3781 aaatgcaatg attgggttag tcaattctca taaatcctcc atttttctga ctaaagtaag
     3841 tactaaacta ctttataata gcgactcacc gataatacga acttctggtt ccaaatggac
     3901 acctgaagtt gattcaacgg tgttaataac atgttcaata agattttcat aatcttgagc
     3961 tgaaccttct gcaatgttaa ccataaaacc agcatgtttt tgactaacct ctactccacc
     4021 aatacgttga cctttaagat gtgcttcgct aatgagttgc cctgcaaaat gtcctggagg
     4081 acgtttgaac acagaaccac aagaagggta ttctaaaggt tgtttcagct cacggagata
     4141 ggttaaacga tccatttcct gagtaatcat gagatgatct cctggtttta aggcaaactt
     4201 agctgatata acaatatctc cactctcttg aatgacagaa tgacgataac caaattgcat
     4261 attcctagct tcaatcgttt tgagttcacc ctgtggtgtc aaaacttgtg cagacaataa
     4321 aatatgagca atttcaccac cgtaagcgcc agcattcata aagactgctc cacctacact
     4381 tccaggaatt ccgcaagcaa actcaaagcc agttaaacta tgataacgtg caatacgagt
     4441 tgtttcaatg agattagctc ctgcttctgc ttcaataaca taaccgttga ctgtaacagt
     4501 tgacagttta tcaaacatga taacaaaacc acggatacca ccatctctaa cgataatatt
     4561 actcgcattt cctaaaacca tccaaggaat attttgactg ttagcaaact taacaatacg
     4621 cgatagttca aggcgattcc ttgggaatgc aagataatca gcaggtcctc ctactttagt
     4681 ataagtatat ttctttaaag gctcatcaaa gcgaatatcg agaccttcta attctttttg
     4741 tattgtttta atcataacct ttatctcaat tctgttttat tgtgttttat agacacaaaa
     4801 aatcggcata aaaccgattt ctattatacc aaaaatatag gttgttgacg atacattatc
     4861 acaatttctg tgtattttaa ttttaaaatc ctaactgact attcgactgg ttttaaataa
     4921 tgaacttctc ctaaattcaa tttttcgaga tagtcttcta gcttagaacc attaggtaaa
     4981 cttagttgag gtgctatttc gagtaacgga actagaacaa atgctcgctt cgtcatatac
     5041 ggatggggta ctactaaatc ttcttggtta atcacttgat taccaaaaag taagatatca
     5101 atatctattg tccttggacc ccagtgctca tgtctcacgc gctttaagcg tttttcaatt
     5161 tcttgacaat aatccaataa ttcaaaaggt gctaagtctg tctctacctg acaacatata
     5221 ttaaaaaaat cttcttggtt agtattcccc caagcagctg tttcataaat agcggaaatt
     5281 tgcgcgactt ttgtcttttg caaatgatca attgaaaaga gagcctgctt cagaaaagtt
     5341 tctctatcac taatattact acctaaactc aagtaaacgg ttgtcatact cgcttgcgct
     5401 ccaattcaat tccaactgat tcatagtgcc cattaattgg tggattgtct tttgtaatct
     5461 ttaatgtaat agcttgtact ggatgaaact gtaagaaaat atcttcaaca ataacacctg
     5521 ctaatttctc tattaattga tactgttcgt tttcaacatg atttttaata cagtcaaaaa
     5581 cttcaccata atgaaccgta tcaattaaat catcagtttg tgatgctttt gctaaatcaa
     5641 gtgatagaac agcatcaact tgaaaaactt gccccaatgt ttgttcttcc gaaaaagcac
     5701 catgataacc ataaaaccga catttattta aataaatctt atccatatta caccaactga
     5761 ctaattactg tcacaatgtc cttatttact tcaacattgt gaacacgtac aatctgacac
     5821 ccctttgcta ttgcccatgc cgataaagca gcagtcccca tatcacgttc agttacttcc
     5881 ctattaccac ctagaagata attaacagta cgttttcgag agatcccaaa taacacagga
     5941 taacctaaat cacacacttg ttccaaacct tttaatagct ctaaattttg ctcctctgtt
     6001 ttagcaaaac caaagccagg atctatccaa atattatcct ttgaaacacc tgcttctaaa
     6061 gcacgctctg ctctttcaag taagaattca caaacttctt ttttgatatc ttgataaaca
     6121 gcttcttcct ggttatgcat caaaataatt ggaacatttc gttctgctgc taaagatagc
     6181 attttaccat catataaacc tgaccaaacg tcatttaaaa tatgtacacc tgcatcaagg
     6241 gctgcttgag ctacctctgt tttataagta tctattgata acaaaagatc agggtagacc
     6301 tctctaatag ctttaatcat tggaactaca cgtttaattt cttcttcagc agtcacaaat
     6361 actgcacctg gccgcgttga ttctccccca atatctacaa ttgccacccc ttgttcaagc
     6421 atctcaccaa cttgatttaa agctgaatca attgtggtat aagaaccacc atctgagaag
     6481 gaatcaggcg ttacatttaa aatccccatg atacaagctt tacctgtaat atcatattgt
     6541 cctattttca tcttttatcc tttttgtatt agcgatagaa tttcttgtct ttcatatcga
     6601 tcgtccttgt ataaaccacg agctaccgtt gtaatcgtct tacttcctgg ttttttaatg
     6661 ccacgcattg tcatgcacat atgctctgct tctatcataa caaaaatacc tttaggcgct
     6721 aaagcatcct ctaaagcttg tgcaacttgt gctgttaaac gttcctgaag ttgaggacgc
     6781 ttactcgcca cttcaactgc acgtgcaagc ttactaagtc cagtaacacg tccatcgttt
     6841 ggtaaataag caatatgagc tttaccataa aaaggaacca aatgatgttc acacatggaa
     6901 tagaatggaa tatccttgac aatcacaacc tcttcatgat tttcagaaaa gactgcagtg
     6961 aattgctcct ttggatcttg attaagtcct gagaacattt ctatatacat ttttgccaca
     7021 cgcttcggtg tatctttcaa cccttctcga tcaggatttt ctcctaaagc ctctaaaaat
     7081 tgataaatag ctttttccat tttttcttga ttgggcatta atttatcctc tcctttttaa
     7141 gttgttgtag ttttgattaa tagtagttcc tgacgaactt gagaaataaa ataaagggag
     7201 ccagtaatca catagaaatc tttatctgat gtcagattta tatcatgtag ccatttcttc
     7261 caatcactaa ctctagtgta cgctttagga tatttgtcta agtttattga tttagggtaa
     7321 tcaaagctag taaccgaaac cggagcgata ctagacaatg attctaacat actctctatc
     7381 ggtttagtat taattgcagc aaatagaata tgcacgtttt tctgctgata tttttttata
     7441 acttgaacga gagcatcgac actttcatta ttatgagcgc catcaatcat tacatttgga
     7501 aagattaatt ctgtacggcc tacccatttt gtcatttcaa gcccatcttt aatggtctgt
     7561 agggttaact ttggatattt gtctcttaat aataaactgg tagttatagc tatgctagca
     7621 ttggaaactt ggtgatgtcc cggaatttgc aactgaatat gatcaatatt tgcttgagga
     7681 cctatatagt taaaataatt ttcctcttct tttatataaa agtcattgtg taaacgataa
     7741 gtctggctgt gggtttcctt ggcttttatt tgaaagactt cttcaacatc ctgacgatca
     7801 ttagcatata cgaatggaac tccttttttt aaaacaccta ctttttgttc agctatatct
     7861 acataagttc gtcctaaaac ctcctgatga tctaatccaa ttgacggaca agtaaccgct
     7921 aaagccttga acatgttggt agaatcataa taaccgccca ttccagcctc aataatgacg
     7981 atatcaactg gacagctatt gccaaaataa tagaacatca aaactgttat aacttcaaat
     8041 tctgtagctg gttctaggtt ggtctcaagg tgcaaccgtt caacaactgg acgaacagtc
     8101 tcaacaagtt tgataaaatc agactctgga atcatttgac catcaatgct aatacgctct
     8161 ctaaagtcaa caatatatgg tgatgtaaat gttccaactt gataaccaga atttgtaaaa
     8221 atatgttgta aataactagt tgttgatcct ttaccattcg ttccaacaac atgaatggca
     8281 gataggttgt tttgagggtt tcctaactgc tctagcatcc atctcatacg ttccaaacct
     8341 ggtttaatac caaaagctaa tttgctatga atccattcta gcgcttcttg ataagtcatc
     8401 ttatttacct ttctttaaca tcgtatgtac ttgtccaata ctaaaaacta aaatagcaag
     8461 ccagataaaa ataaaaccta ttacctcccc actaacgata gtttctttaa aaataaacaa
     8521 tgctagcaat aattggatgg ttggattgat gtattggata aaaccgataa tatttagagg
     8581 agccctctta atggcttctg caaataataa aagaggaact gctgtaatga ttcctgaaag
     8641 tgagagtaaa actaattgta atatattata atcagttaaa aaatctttgg cgaaaaacac
     8701 tatataaatg agtgcaaacg gagcaataaa actactttct accagcatcg aaaaatcgct
     8761 agataaagaa attgattttt ttaataaccc ataaaaacca aaagaaagcg ctagagttag
     8821 tgaaattaaa ggaaaatgac cagtttgata aactaaaatt ccaactccca tgattgcaat
     8881 caaaattgca aggctaacta ctcttgccag gtgttcacgt aaaacaagga ccgacaacaa
     8941 aattgaaata atcggcatca tataataacc aagacttgcc tcagtagcgt gaccatgcgt
     9001 gactgcaaaa atatagacca accaattaat tgaaattaaa atactggcta caaacattgt
     9061 ccaaaatgac tttttattat taactaagcc ttttaagtct tttaaataga ctgtttttct
     9121 acccgaaacg agcatataaa taagcatagt taaaacagtg aatataatcc tataagcaaa
     9181 cgtactatat gcttcaattc cagataataa tttccaataa agagataaaa gcccccataa
     9241 aacataggct gacaagccaa ggataattcc taaattatct tttctcaact ctcactcctt
     9301 ttcgctcaat attaagtact tgaatctgtc catctaaaca taatttactt aactctgtga
     9361 caattggttg ttctttttct ttcgaacaca gaaccattac cgtcggtcct gctccagaca
     9421 aataagtagc atacgcaccg ttttgagtgc tgatttgttt tataactcca aattccttca
     9481 ccaaaggttg acgatatctt tcatgaaaga ggtccctttc tatagcccat ccagcagtta
     9541 ccaaatcacc tttcaacaac gacgcaacca tcacattagc gacgctgctt gaagcaactg
     9601 catcctcata actcaatttt tgaggtagta catttcgact atcacttgtt tttaattcat
     9661 aatttggaat aaaagcgata aaggaactat ctggaaacat cacttccaaa cttttaatat
     9721 catttttcac tattgatgaa ataactaaat caccaaaaat ggccggtgct acattatcag
     9781 gatgtccttc taatcgggta gctatctcta atttttcttt ttgagaaaga gccaaatttc
     9841 ctaattgatt ggctaattct attcctgcaa caattacaga ggaagaagaa cctaaacctc
     9901 tggccaaagg aatatctgaa aacattttta agcggtgtgg tgccaaatcc gacttaacat
     9961 gcaaagctgt ttgaataagg aggttagtat gatcttttgg aatatttact aaattgtgtt
    10021 caactaacca ctctgtactt tcttctaata cttcaatgct taaatacttt gataacgcaa
    10081 ctcctattga atcaaagcct ggaccaatat tcgcactagt tgcaggtaca attatcctca
    10141 tttcaccctc ctattacttt gaaaacattg attaactgaa tgacaccact ctcaggaaac
    10201 tcctcaataa attgagttat tttctcttgg ctaacttcat ctgttaatac atagatttct
    10261 tgttgtttat catccaatgc tttttcaata acatttttca aacctatatt ttcttgctca
    10321 aagtaccttc caagtgtatc actatcttca gcatttctca aagaaatagc tagatagtac
    10381 tcattgaaga tatcagactg cattgacaac ctacacggac aatgataagt attcataggc
    10441 ttaattttta tctgtgatcg aatactacga ctaatatcga tgatatcggc taaaacagag
    10501 gttgctgttg gattttgtcc cgcacctggt ccataaaaaa gagactgacc aatccctatt
    10561 gattcaatga atactgcatt catcacatga ttaactgcag ctaagggatg ctcttgagaa
    10621 attagtgtcg gagaaacgtc tacacttata ccagttggag tttcacgaag atcagttacc
    10681 aacttaatgg caaagcccat ctcctgagct acttcaacgt cctctgagcg aatactagaa
    10741 atccccttat aattaacatc atcaaaatca ataggcatac caaatccaaa ttgacttaag
    10801 atagtggctt tgtaggcagt atcaatacct tcaacatcat ttgtaggatc actttcggca
    10861 taacctaact cttgtgcctt ttttagagct tgttcataag accatccctc ttcaaacatt
    10921 tttgttaaca tgaagttgga ggtaccgttg agaattccta ataaacgtgt cattttatct
    10981 gaggcaaacg aatgcgatag ggtccttaaa ataggaatgc ctccagcaac tgctccctca
    11041 tagaataaag ctagacctct agcgtctgct aaatccatca actcttttcc gtgtaaagca
    11101 atgagatctt tattggctgt cacaatatgt ttaccaaatc ctaatgcttg actcaaataa
    11161 gttttagctg gctcaatccc ccccattaat tccactacaa tatcaatttg tggatcttga
    11221 agaatagtat ttatctctgt cacaaagtta taagtaaatc cctgattgat gaaacgctgg
    11281 cggctttcat tatctcttac taatacttta tcaattacaa tatcttcgcc ttctaaagaa
    11341 agtagcttat gttgattttc ttttagcaaa tatggaatac ccttagcaac cgttccaaaa
    11401 cctaataaag ctattttaat agtcatgaat ttctccttag aaattgtctc taacttcaag
    11461 acaaacattg gctatattat aacaaattac caatattttt tatagggtaa acgcgtcttg
    11521 tcctatgaaa gttcttaaaa agagaatatt atttcagaaa agatgataca ataaaagtac
    11581 tttaaaagtt aaggagaaat aaatggcaca cacacctact agtcatcgta aaccacgaaa
    11641 acgttcccca tggttagcta ttgcttctgt cttttttttg ttaatagctt taatcggcat
    11701 atttctattc ttcaacaatc gttcaaagca agaaataaaa acaaaaacga acgcctctag
    11761 tcatcgaaaa attgtcacat ctatcaaaaa aaagaaatgg gttaagcaaa agactcctgt
    11821 taaaataccc attttaatgt accatgctgt tcatgttatg gacccttctg aagccgccag
    11881 tgctaactta attgtggcac ctgacatttt tgaaagtcat ataaaacgtc tcaaaaagga
    11941 aggatattac ttcttagccc ctaacgaggc atatcgtgca ctaaacgaaa atgctttacc
    12001 tgaaaaaaaa agtcatttgg attacttttg atgatggaaa tgccgatttc tacactaagg
    12061 cataccctat actcaaaaag tacaaggtta aagccactaa taatatcatc actggctttg
    12121 ttcaagaagg acgagaatct aacctcaacg ttcaacaaat gctagaaatg aagcaaaacg
    12181 gcatgtcatt ccaaggacat acggttactc accccaatct ttctctctta actccagaac
    12241 tacaaaccca agaaatgaca ctatcaaaac tgtttttaga tcaaaaactc tctcaagata
    12301 ctcttgcgat tgcttatcca tcaggacgct acaatcctac aactttggat attgctagtc
    12361 aatattataa actagggctt acaacaaacg aaagagttgc aacaaaagat aacggtttgc
    12421 tttctcttaa tcgagttcgt atcttaccaa ctactagcga tgatgatttg atcaaaacga
    12481 ttaaccaata aaaagaagcc ttctggcttc tttttattct tcataagatt taaatggttc
    12541 atttttacgt ggataacgat ctggagaaat cgataagccc atttcttttt tctcatggaa
    12601 aagctccact aatagcgcta gcatcatcaa aatcattacg aatgaaaatg gcagagcgac
    12661 aataattaaa acgttctgca gcgctaacag accaccagac aagagtaaga caatagcaat
    12721 cactgccaac actagccccc aaattacttt agttcgattt tttggattta aattaccatc
    12781 ttctgtcaac atagccaaaa cgtacgttgc agaatctgct gatgtaataa aaaatgaaaa
    12841 aattaaaatg atagcaatag tggataaaag ccaacctaaa gtgtagtgat taaaagtagc
    12901 aaacaatact tcctctgttg caaactttgt caaattaata cccagttgtt gtacctgagt
    12961 tgataatgtg ccaaatgctg caaaccatac aaaactcaat aatgtcggta ttaaaagaac
    13021 taccgttaaa aactcccgaa tgctacgccc tttagaaata cgagcgatga aaactccaac
    13081 aaaaggagac caggaaatcc accacgccca ataaaaaatc gtccaattat caatccaaga
    13141 acgtttagta ttgtcaaaag cagctgcacg aaagctcatt ccaaagaaat tttgtaaata
    13201 agagcctaaa gactctgtta gggtatcaaa aatacgaacc gttggtccca atacaataac
    13261 taaagctaag agggctaccg ctaaaatcaa atttaagttc gataaaattt taacaccttt
    13321 tcctaaacct gataaagctg acataacaaa taaagctgtt gtaataagga taataacaat
    13381 ttgaacaaat gcattattgg gaacacccaa taaaaaactt aaccctccgt tgatttgagc
    13441 agctccaaat ccaagtgtcg tagcaactcc aataactgta gcaacaactg tggtgatatc
    13501 aacaattttt cctagtcaac catctgtctt atcaccgaaa agaggcttta aagtgacaga
    13561 caagaggtat ttctcttgct ttcgaaatcc aaaataagct agagccaaag caaccaatgc
    13621 atatactgcc caagcatgta tcccccagtg aaaaaatgta aaacgaaatg catcagctaa
    13681 tgctgtttgc gattccttag gtgcgccagg tgtcgaaata gcaaaatgcg ataaggggtc
    13741 agctgctccg tagaagacca aaccaatccc catacctgct gaaaacatca ttgctatcca
    13801 tgatacagtt gaatattcag gacgtgaatg aggtcgccct aaacgtattt gtccaagagg
    13861 actcataata aaaaagacac acaaagcaag aattgaagta actaacaaca agtacaacca
    13921 accgaaattg gtagatacct gttcacgcaa gatttgtgtc caagattgaa aaccacgagg
    13981 aacaataatt cctaataaga ctaaaataag cgatacaatt agtgaacctg taaacacagg
    14041 cgtaatatgt tttttactca aaatttcctc ctatactaaa atagtatagc atactttaat
    14101 aaatactcct ctcattttaa tcacctgtat caagacctct ccttgtctcc tcaacaaaaa
    14161 agaaccagat aacattatct ggttcttgtg tttgacttag tgattaatca ctatttggtg
    14221 taaataccaa atgttcgttg acaaaggtat caaatccaag ttggctcaat tcacgaccat
    14281 atcctgaatt tttaatacct ccaaacggta attccggtaa tgatgtccac cctgaattaa
    14341 taaatgtcat ccctgtttca atctgagccg ccactttttt agcatgttct ggatcagaag
    14401 agaaaacagt acttcctaaa ccataactag aatcattagc taaagcaata gcttcttctt
    14461 cagtatccac tttatagata gaggctacgg ggccgaaaat ttcttgatta tagattggat
    14521 ttgctttagt gatattcgtt aaaacagttg gcatgacgaa gtttcctgga tgatctatag
    14581 tgtcatttcc aaaaactacc tcagcaccgt ggtctaccgc caatttaatt tgttttaaaa
    14641 catcatcctt tgcacctgca gatgacagtg gagccaaggt tgtttccgaa tccattgggt
    14701 caccccattt agcagactta aaggtctcaa ctaccatatt tacaaattcg tcatacttat
    14761 cagccataac aatgaaacgt tttgaagatg tacatacttg acctgcatta tataaacgcg
    14821 caaagaatat tgttttacta agtaaatcaa aatcagcatc atctaaaata agaaaagcat
    14881 cgttcccacc taattccata gacgattttt tcaaattttt accagcctca gctgctattg
    14941 atgccccacc acgctcagaa ccagttaaac acacacctgc aacacgagga tctgatataa
    15001 ggtttgaaac ttgatcatac gaagcaaaaa tatttttaaa cgctccttct ggagctcctg
    15061 cttctcggac taaatcctca aatgcttgag ctgatgcagg acaaatagaa gcatgtttta
    15121 aaagcatagt attacccact ataaagttag gggcaaatac acgcataatc tgataaaatg
    15181 ggaaattcca tggctcgaca gcaagtaaaa caccgacagc ttgtttcaaa tagtaagcct
    15241 ctccatttgg actttctaac ggaacaggtt ttaaaaactt ttgcccatta tcagcataat
    15301 agtcagctat gtctgcacac aagtccactt ctccttgtgc ttcagtgaaa agttttccca
    15361 tatctttagt cataacttca gcgtacttat cacggtcctt acgtaataaa tctgctactt
    15421 tatgcaattg attttggcgt tcttcaacat tatcttcttt gcgccatgtc ttgtagaggg
    15481 tatgcgctat gtctaatgac tgttcgagat cactgtcact gatattatcg aattcatgca
    15541 atacttcgtt agtgtacgga taaattgttt tatatgccat aatgttccct ccttttgata
    15601 tcctcattat aacatttatg aaatcgatta caaatattta gctttagaca agatcaccaa
    15661 gttccaagga tagcttgcat tgttaaacta actataataa tgcctaacca acacgctgca
    15721 cctaaaagaa ttgattttcc tccagtttta attagtttgc tgacattagt atttatacca
    15781 attgccccca ttgccataac aataaaaaat ttagataaaa ccttaagtgg ctcaaagata
    15841 cgtaacgaaa aacctaatga agcaactatc gttgttagca aagaagctaa gataaaataa
    15901 agcacaaact tcggaaatat cttagtaaca cttacagatt gttctttctg cgtttgtttc
    15961 atatgataaa tagataatac aatcgtaatt ggaatgatgg ctaatgttcg tgtcaattta
    16021 acaatagtag ctccgcccaa agtattgctg tgattgatag catcccatgc agtagcagtt
    16081 gctgtaactg acgaggtatc attgacagca gtccctgcaa ataatgcaaa accgtgatca
    16141 gagagaccaa taaaattccc cagcgttgga aaaattaaag ccgctaagat attgaataaa
    16201 aaaataaccg aaatagcttg tgctacttca tcatctttag cattaataac tggtgcagtc
    16261 gctgcaattg cggatccacc acaaatagat gaaccaacac ctattaaagt agcaatcgtt
    16321 ttatctaact taaataactt ttgcaaaaca taagcaataa tcaatgaaat ggaaatcgtc
    16381 ataataatga tgggtaacga tgatatacca actttcatca cttgcatgag gtttagtcca
    16441 aaacccaata agacaactgc tgtttgtaaa atatatttag acgtaaaagc aataccctta
    16501 ttaaacaatt gtcgattttg ataaaaggat ccaacaatta ttccaatcaa gatagcaaag
    16561 actggtgctc ctattaaagg gagataaagc ccaagcaacc aagatggtat agcaataata
    16621 aagcataata ttagtccagg aattttttcc ttaaataaca tgatagtcct cctcacattg
    16681 actattatat cacaagcttt aaaacgtggc aggaaaaccg aaagaggacc agaatatgct
    16741 ctgatcctct ttcattacta attcacaatt atttagttac tttcataaat ggatatgatt
    16801 tttcgttacc taaaatcaat tttgagccct cttttttata ggctgttttt tcaccatttt
    16861 tcacaatatt accatagaac gcatttgcct ttttattgat atgcgatact ttaataacac
    16921 ttgttaagcg atgtacttta ccatccataa ctggcaatgt catttcacca gtattagtat
    16981 tatattttcc tccgacagct tgtgcagcac tttggaaact tctatcaatt tgttggtcaa
    17041 tttgtttgtc agtgattttt gtttcatcaa aaatcttttt acctgcttca cttaaatcat
    17101 tgtaagtcaa accttgatct tttaattgtt tttcaagagc tttatcaata aaagttttta
    17161 tagcttgatg gaattttacc tcatcaattt gagcagtgac tgttatcttt gcctcatcgt
    17221 ttttgacgtt catatgcata ttagcgccct taaccaaatc agctgcacta atccccatat
    17281 ccgctgcctg acttccaagt tgttgttcta tttctttctc aacttgcttc tctaaagaaa
    17341 ctgtacgcca tttcccctct agtgactgac gattattcca gaaatagaga ccgcctgcac
    17401 ctacaattag aataagtact agtacagtta aaaaaacttt taaaaacttt ttcatgataa
    17461 aaactcctta
//
LOCUS       DATROY010000029        17160 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.029, whole genome shotgun sequence.
ACCESSION   DATROY010000029 DATROY010000000
VERSION     DATROY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17160
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..424
                     /locus_tag="VBD11_000811"
     CDS             <1..424
                     /locus_tag="VBD11_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953776.1"
                     /translation="EFKESLNLNESFYLQLKKEEKKPSDNLKKEEKPRENSVKERETP
                     AENNDFVSVTEKNNLIDKYKELLSKIPENTQNPGEKNIRNYLEKEYEELLQKDRLFKH
                     EYTEFTKSLNLNETFYSQLKEGEMKLSENPEKGETNTN"
     gene            complement(596..1210)
                     /locus_tag="VBD11_000812"
     CDS             complement(596..1210)
                     /locus_tag="VBD11_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6953777.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(1343..1768)
                     /locus_tag="VBD11_000813"
     CDS             complement(1343..1768)
                     /locus_tag="VBD11_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6953778.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(1895..6301)
                     /locus_tag="VBD11_000814"
     CDS             complement(1895..6301)
                     /locus_tag="VBD11_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6953779.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(6424..7008)
                     /locus_tag="VBD11_000815"
     CDS             complement(6424..7008)
                     /locus_tag="VBD11_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6953780.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(7133..8986)
                     /locus_tag="VBD11_000816"
     CDS             complement(7133..8986)
                     /locus_tag="VBD11_000816"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6953781.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(9078..10337)
                     /gene="rseP"
                     /locus_tag="VBD11_000817"
     CDS             complement(9078..10337)
                     /gene="rseP"
                     /locus_tag="VBD11_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6953782.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(10368..11162)
                     /locus_tag="VBD11_000818"
     CDS             complement(10368..11162)
                     /locus_tag="VBD11_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6953783.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(11177..11929)
                     /locus_tag="VBD11_000819"
     CDS             complement(11177..11929)
                     /locus_tag="VBD11_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6953784.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(12096..12437)
                     /gene="yajC"
                     /locus_tag="VBD11_000820"
     CDS             complement(12096..12437)
                     /gene="yajC"
                     /locus_tag="VBD11_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6953785.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(12514..12858)
                     /locus_tag="VBD11_000821"
     CDS             complement(12514..12858)
                     /locus_tag="VBD11_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6953786.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(13049..14212)
                     /locus_tag="VBD11_000822"
     CDS             complement(13049..14212)
                     /locus_tag="VBD11_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6953787.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(14237..15574)
                     /locus_tag="VBD11_000823"
     CDS             complement(14237..15574)
                     /locus_tag="VBD11_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6953788.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            15728..>17160
                     /locus_tag="VBD11_000824"
     CDS             15728..>17160
                     /locus_tag="VBD11_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953789.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQ"
BASE COUNT         5612 a         3513 c         2679 g         5356 t
ORIGIN      
        1 agaatttaaa gaaagtctaa atttaaatga atcattctac ctacaattaa agaaagaaga
       61 aaagaaaccg agtgataatc tcaaaaaaga agaaaaacca agagaaaatt cagtaaaaga
      121 gagagagact ccagctgaaa acaatgattt tgtttcagta acagaaaaaa acaacttgat
      181 tgataaatat aaagaattat tatcaaaaat cccagagaat acacaaaatc ctggggaaaa
      241 gaatatacga aattatctag agaaagaata tgaagaacta ctacaaaaag atagattatt
      301 caagcatgag tacacagagt ttacaaaaag tctaaatttg aatgaaacat tctattcaca
      361 attaaaagaa ggagaaatga aactaagtga aaatcccgaa aaaggagaaa cgaacacgaa
      421 ttaacctttc aaatatctac atcaaataac agaagtctcc gtatcaagcg aattgtagct
      481 tttttgaata tactaaaaac actcctacct ctattttact agaagtagga gtgttttttt
      541 cattttagtt catagatgca gtgccattgc tatccttgcc tttttttgta cagtattatc
      601 cgatgacttt aaagcggtca tcatcattca tgaattcttt ttctcctgtt tcagcttcga
      661 ttgaaccaaa atttagttgt ccacgaagtt tccaactagc tggaacacca taaacagctt
      721 gtactgatgc atcaataact ggattgtaat gttgtaaatt accaccaagt ccaagttctg
      781 cagaaagggc tgtccaagta tttactgagg caatacctgt tgattgctca gaccaaactg
      841 gaaagttatc cgcataaaga acgaattgtt cttgaagaga tttgacaaca tcttgatctt
      901 caaagaagag tactgttccc tttgaagctc caaaactagc taatttttcc ttagttccag
      961 caatagctgt ctctggagca ccttgtactt tcattgtttc taccaaatca ttagcaacca
     1021 attcatccca gaacttagta acttcatcat tcaataaaat gacaacacgg gaagtttgac
     1081 tattaaaagc agatggggat tggcgaactg cctctttaat aatctctact attttctcat
     1141 cagaaacttc tgtattgcga ccaagagcgt aaatagagcg acggtttttt aattcttcca
     1201 aaaatgacat attttttctc cttgttcttc aatctcagat atcttactac atttgttcta
     1261 gtgacatatg aattagatta aagttattta ttactatgta gtaattatat taccactata
     1321 tcgtaaagtc aaagaatgtg actcacaagg ttttaaattt ttttaaaagg ttaatcaagt
     1381 cttcttgctc tgtttctgtt aagactgaaa aagcatcttc tacacgttta atatgctctg
     1441 gtagagcttt ttttatcact tcttctcctt cagttgttaa actcaccaaa aaggctctct
     1501 tatcattcgg acaagaatgg cgtaataccc atcccttctt ttccatattt ttaataacaa
     1561 cagtcatatt acctgatgta gcaagcatat tttcaatcaa ttctccaatt ttcattgtgc
     1621 ctttagcata gagaacatcc aaaactgcaa actgagttgg tgttaattca aattctttaa
     1681 atgattctga gacttttccg tctattgttc taaatgcttt tcgcatgaca accatagatt
     1741 taaccgctga atttttaaag tttcccattt catctcccaa acgtattttt tcttatttta
     1801 tcactaatta gttattttaa gcaagaatat catctttgcc ctatcaaaag cagctggcaa
     1861 ataataaggt tgagtaacta ctcaacctta ttgcttagaa gaaatcatca aaaagactca
     1921 actgattgtc ttctggtaaa tttcccaaaa tacccatctc atcaaatttt tcaactaagg
     1981 tacttgagac tcctcctcgt ttacgaagct ctgtttttga taaaaattca ccatcttcac
     2041 gtgctcgaac tatctgtttt gcaacgtttt ctcccaagcc ttccatagca acaaatggag
     2101 ggattagcgt atcctcttca ataataaagt cagtcgcatg gctacgataa agatctaatt
     2161 tcccaaattt aaagccacgc tcaagcattt cattaaccaa ctctaatgtc gtaaaaagat
     2221 cattttcgac attcgtcgcc tcattacgct gacgcttctc agtgatatcc ttcatgcgcg
     2281 ccttaacggc atcaagtcca gcactcattg tgcgtaattc aaaagctttg gcgcgaatag
     2341 aaaaataagc acaataataa aatattggat aatgtacttt aaagtacgcc acacgtaagg
     2401 ccattaaaac ataggctgct gcatgggctt tagggaacat atacttgatt tttccacacg
     2461 attcaatata ccaatccggg acattattat cacgcattgc ttggatataa ccgttacgct
     2521 catcttctga aattttcaac cataacccct tacgcacgcg ctccatgatt gtaaaggcca
     2581 tctttggttg tagaccagcg tgcattaaat aaaccatgat gtcatcacga caaccaataa
     2641 cggttgataa ggtagcaata ccttctttga ttaagtcttg agcattacct aaccaaacat
     2701 ccgtaccatg tgatagtcct gacagttgca acaattctgc aaatgtggtt ggatgggttt
     2761 cgttcaccat accacgaaca aagttcgtac caaactcagg aataccaagc atccctgtag
     2821 gagttccaat ttgctcttct gtcactccta aaacttccgt accagaaaat aacttcataa
     2881 cgtctggatc atctggaagg atatttgatg gatcaattcc tgataaatcc tgaagtttac
     2941 gaatcatagt aggatcatca tgtcctagaa tatctaactt caagacattc tcatcaatat
     3001 catggaaatt gaagtgagtt gtctgccaag cagctgtcat atcatctgct ggatattgaa
     3061 ccggagtaaa atcataaaca tccatatagt taggaataac aacgatacca ccggggtgtt
     3121 gacctgtaga acgtttgact cccgcagcac cagtcgctaa ccgttcgacc tctgcatcgt
     3181 tataaaattt attgtaatct cgttcgtaac ctttgacaaa accaaaggca gttttttcag
     3241 ctacggtacc taccgttcct gcacgaaaag catattcttc tccaaagata tcacgaacat
     3301 ctaagtgagc agaaggttga tcatctccag agaaatttag atcaatatca ggaaccttat
     3361 ctccatcaaa tcctaagaaa gtttcaaacg gaatatcctg tccatctttc ttgtacaaag
     3421 taccacattt aggacaattt ttatttggta aatcgtaacc tgaaccacaa gaaccatctg
     3481 tgataaattc agaatgctga caatttgggc aaacataatg aggtggcatt ggattaactt
     3541 cagtaattcc tatcatggtc gctacaaaac tcgacccaac agacccacgt gatcctacta
     3601 gataaccacg ttcattagaa cgttgcacca acatttgcga agcaagatag ataacggcaa
     3661 atccattacc aagtatagat gctaactctt tttcgatacg taaatcaata atatcaggaa
     3721 gaggattacc atatatttcg aatgctttag cataagttaa ttcagcaact cgttcttcag
     3781 cacgatcaac aaacggggta tagagatccc cttttacaac ttcaacgtct tcaaatcgat
     3841 ctgcaaaagt attcgtattg gttacaacga tctcataggc taaatcttta ccaagaaagg
     3901 caaattcatc taacatctca tttgttgtac ggaaatgtgc ttttggcaaa ggcgctggtt
     3961 gagcatcctc cccacgaccg atagtcctat taatcatggc accttgtcca agactacgaa
     4021 caataatctc acggtaaatc tcatcttcag gctcaatata atgaacattt cccgtagcta
     4081 aaacaggctt atctaaacgc cgaccgacct ctattaggtc tctaataatt tgttgaatcc
     4141 ccgcctcatc tttaattaaa tcacgaacca ccaaggggcg ataaatagca ggtggcatga
     4201 cttcaataaa atcataatat ttagctaggg tcactgctgc atcaattcca tttgataaca
     4261 aggcatcaaa aacttctcca tcagaacagg cagtacctag caagagacct tctctgtgag
     4321 catctaaaac tgatctagga attcttgcca ccccttcaaa atatttaaca tttgaaaggc
     4381 taactaactt aaatatattt ttaagtccaa cctgattttg gacataaatg gttgcatgct
     4441 ttatgcgtgc ctttttgtaa gagtcttcag ctaccaattt agtattgagc tccatcaaat
     4501 tagtcacatc acgattttcc cgcgcctctt ttaagaaaat aaagagtaag cgacccgttg
     4561 cttcagcatc gtagttggcc atatgatgat gttccaaagc aacttggaaa cgtttggtta
     4621 gaggccccaa accatgacgc ttatattcag gataaagatt tcttgcaaat tctaaggtat
     4681 caatgaccgg ctgtgtgatc aaaggcagat tatgacgttc ataattcgca ttcataaaac
     4741 caacgtcaaa ggttgcatta tgcgcaacta aaaccgtacc ttgacaaaaa ttctgaaatt
     4801 cctgaaggac ttgtaaaata ggttttgaac ctctgacatg attatccgta atacctgtca
     4861 actcagttgt aaagtcagaa agaggatggc ctgggtcaat aaactcatca aattgctcaa
     4921 taatattacc cttgaacatt tttgatgctg caatctgaat taggtcatta ttagctgcag
     4981 aaagacctgt agtttccaca tcaaatacaa cataagttgc ttcgtgcaaa ttcatatcaa
     5041 cttcgttgta ggaaataggc actttatctt caacaatatt tgcctctaat ccaaaaattg
     5101 cttttatacc tgcttttttt gctctgtggt agccgtgcgg aaaactttga acattggcat
     5161 ggtcagtaat tgcaatagct gggtgtcccc acttagctgc agtatcaatg aggctctcga
     5221 cagttggtaa agcatccatc gttgacatat tggtatgagc atgaaattcc acacgttttt
     5281 gatctgctgg cattaagtct ttacgttcat gatgcacaat ctctttgata tcttgaacat
     5341 tcattgtcaa agatttagta aagttattgt tctcaatatt ccctctgacc cgcaaccacg
     5401 aacctttaga aatcatatcg tacttcttca attcttcatc atctttagcc cacttttgca
     5461 tagcaaaact tgaggtataa tcagtcatct taaaattaat gatatgacgt ccagtgcggg
     5521 ttgtttttcg ttcaacacta aaaaccattc cttcaaaaac gatacgattt tcttctgtgg
     5581 tcacctcaat catgggtgta atctctgctt tttcaaagcc agcttgacgt tgtttgatac
     5641 gctctttgaa atcataattt tgagttggtg tcacttcttc tgatggaggc gctgaatctt
     5701 ctagtgattt ttgtgcttcc aaggcttgca tagcctcttg gttagctttt tcaagcaatt
     5761 gctcgcggtt agtttcaaat gatgatttta agttttgcgt catttgttca tcagaaacca
     5821 tatcaatagc aagtttacca aatccaaaca atgaaaattg ttgttccaag cgcggcaaat
     5881 gattctgacg aaaatgatta ttattgacaa attgaggtgc tgaaataatc atctgactgc
     5941 cattgtaatg aacttttaat tgtgaaaaag aactcttaaa acttgcacta ttacacaagg
     6001 gttcacaaaa agcttgttga taataatcct gtagcagatc atcagaaaaa tcaatagttt
     6061 ctgctcttat atcaaaagta gcctttatat ctgctttttc aaaactattc actaaacgag
     6121 tttgtaattc acaatacacc tcaattggca ataattctgg aaatgaaaaa tgaaattccc
     6181 ataagcgtga tagagaatga acctttacct cgataatatc agcagacgaa aaaacacttg
     6241 aattcttaat ttcaagcggc atttcaatct gatccataag tttcttgaac aattctgaca
     6301 tagttttctc ctgaatatct tacttccatt ttatcacata tctgtaaata tttcgagata
     6361 acaaaaggag gctgttaaaa aaggcattta ttagccttct taacaacact ctcttaactg
     6421 agactattta tcataagcat ctagactgta ggtttcaata atttggttta atttactagc
     6481 ataagcagta tctgtcgcat aaagacctgt tagagctgca gtagcatcct tataagaaga
     6541 agtatttgat ttccaaacag atgcatactt ttgactagat actaactcag cataatcata
     6601 tagtgaagca gaataatttg gataggcgcg aaaaggagct tggatttgag tcatattgcc
     6661 tttcccatca tcttctaaag taggcatttg gacagattta cctttatatt ctcctttgat
     6721 gccaaagagg ttataattag gagccttaga caaatctgat tgtccactgg atgattccaa
     6781 aatagcttgt gctaataaga ctgacgcata caaatcatag gtcttagaaa tttcaatagc
     6841 tgttggagaa atcgtttgaa taaaagtcgt cgtttgatcc gatgcactag tgactgtcgg
     6901 ttcgacatta tcatttttat aattgtaaaa ccaaacccca cccaaaccaa aaactaatag
     6961 tgttgttgtt aacctcaata ccaatttatc tttttttcga cttttcatga tggctccttt
     7021 ctaaaatatc atttcattat aacaaaaggc agttgctttt taccaataag aaccatacta
     7081 aatttcactc catataaaaa agttagcaca tcagactaac ttttttatcg gcttattttt
     7141 ttgttagaat ctctaaagtt tcaattaagt tatcagcgtg aacctcaatc gtatcaccac
     7201 ttgctttaat tttcacttca acgatacctt cagaagcttt cttcccaaca gttactcgaa
     7261 ttggaagtcc aattaagtca ctgtctgaga atttagaacc aacacgttca ttacgatcgt
     7321 ctgtcaggac ttcataacct ttaagcatca aatcagcttc aattttctca gtaagatctt
     7381 gtgactcttg atctttgaca ttgacagtaa tcaaatgtac atcaaatggg gctaattctt
     7441 ctgggaaatt aatcccccaa gcaaagcgat aagctccttt tggtgtctta ttcacaaaaa
     7501 gtcgggcatg ttgttcaatg acagctgaga gaatacgact aacaccaata ccataacacc
     7561 ccataacaat aggatttgag cggccatttt catcaaggat gtttgctccc atactatctg
     7621 aataacgggt acctagttta aagatatgtc caatttcaat accacgagca aatttcagag
     7681 tacctttacc gtctggagaa atctcccctt cttttacttc acgaatatca acgtattcag
     7741 ctgtgaaatc acgttctgga ttaactccag taaaatgata accatcttgg ttagcaccag
     7801 acacagcatt agcaaggtct tgaactttac gatcagcaat aattttaaca gactcaggta
     7861 ggttaactgg cccaagagaa ccaaaacctg caccaaaaat ctcttttgct tgctcttcac
     7921 ttgcaggctc taagaaatca gccgctaaat aattttttaa cttcacatca ttgacttgat
     7981 cgttaccaac tagtaaagca accacgggct gttcatcagc aataaacaat agtgtcttga
     8041 tagtttgatt ttcatcaatt gataaaaatc cagctacctc atcaattgat ttgcagttag
     8101 gagtttctac ttttgttact tcttcaaagg tagcagcctt agtagatggt ttgtactcat
     8161 tcgttgccat ctcaaggtta gcagcatagc ttgattcagt ggagtatgca atagtgtcct
     8221 caccagaaac tagccaagcc gagagttctg ccttaatttc ttctaaaaca tcctctggaa
     8281 tatcatcaat agaaggaata gttttatcaa ggactaacca acgatttaaa tctgtacgat
     8341 tgggagttac tgccataaat tcttggctat ctttacctcc catagcacct ccgtcaccta
     8401 taatgccttt aaagtcaagt ccagcacgtg tgaagatggc ttcatatgct ttacggtaat
     8461 cttcgtaagt gacatcaagg tcttcataat ctttgtggaa gctgtatcca tctttcatga
     8521 taaattcacg tgttcgaaga agtccattac gaggacgctt ctcatcacga tatttagact
     8581 gaatttgata aaggtttagt ggtaattgct tataagattt tactgcatca cgaacaagag
     8641 ttgtgaaagt ctcttcatga gttggtccta agataaaatc cgattgatca cgattcttta
     8701 atttataaag gtcttcacca tatgtttcgt agcgcccaga ttcacgccat aaatctgctg
     8761 taagaagagc tggagctaac atttcaactg caccaatctt ctcaaattct tgacgcataa
     8821 ttgttttaaa tttttcaata gtacggttag ctagaggcaa ataagcatag attccagccg
     8881 aaacttggcg gacatatcct gcacgaacca taagagcatg gctgataacc tgagcatcac
     8941 ttggcatttc gcgaagtgtt ggaataagca ttttactttg tttcattaca ataagatacg
     9001 actaatcaaa gttgattctt tttcaattga gaattgcaat tgaaaaattc gtatttcctt
     9061 tcacgtttta ttatctttta gaagaaagct cgcatgatat cgttccaggt tacagcaatc
     9121 attaatacta acataactgc taccccagcg agcgtaatat aagtttctgt ttcttgttta
     9181 agtggtttgc gacgaatggc ttctaaaata ttcatcacta tcttaccccc gtcaagggct
     9241 ggtataggga tgagattcat aatacctaag ttgatagaga ttaatcccat taagttgaga
     9301 actgtgacga aaccattttt agcagcttgg ctactagcct gatagagagc tactggacct
     9361 cccaatttat taatactaaa atgagcaata agtttcttta attcatttaa aattctaaag
     9421 aaactttccc atgctagttt aaatccacct aaaagcttgt ctttaaagct tgtctttaga
     9481 gctggcataa tgccgatgat gtaggattta tcaactttct gtggcttaac gttgatcgtt
     9541 ttcaccacct ctttgctctt aattttaagg gcaagttttt ggtttttttc taaatgacta
     9601 gttgactttt ctactgctgc tgtcaactgt tcccagttag aaactttgtg cgaccctatt
     9661 tgtaaaatac ggtcattatt ttttaaacct gcactagcag caggaccatt ttctgataca
     9721 cggacctgat tggttgataa atcttgaaca cctccttgaa taaaagcaag tgcaataaag
     9781 actacaagac ctaaaataaa attattcatc ggacctgcaa aattggtaat taagcgcccc
     9841 catacagaag cattctgata ctgcacatct aagggagcaa tacgaatttc agtaccatcc
     9901 tcctcaatga tagtagcatc gtgatcaaca ctataggttt ttgtctcact taagaccaat
     9961 cctgtgatag ttagtttatc ttctaaatca taagctgtta cattgattgg taaagaagta
    10021 ttgtctaatt gttttcctga taaattaata cgagttacaa tgccttcttt atttaaagta
    10081 aggctagccg gcgtccctgt cttaatttca gtcttatcat caccccaacc agccatacgg
    10141 acataaccac ctagcggtaa tatacgaata gtataggttg ttccttcttt atcaatgtga
    10201 gaaaagattt ttggacccat accaatagca aattcacgaa caagaattcc tgatttcttt
    10261 gcaaaataga aatgcccaaa ttcgtgaaca actacaatga caccaaaaat aataataaag
    10321 gttaaaattc ctagcattca attcctctct aaatacaata tattatttta gaataagcca
    10381 aagaagtgca tgattgggaa aacaaaaatc atactatcaa agcgatccaa aatcccccca
    10441 tgacccggaa tcaatttacc cgaatcttta acaccaaaat gacgtttaat actactttca
    10501 actaaatctc caaattgacc aaaaatgcta aagatagcca ctaaaactaa catgactagg
    10561 aagctatgag gcgcatagac ggtcttatca aaaagcataa aaaagaaagc taccacaatg
    10621 gctgaagcaa ttcctcccag actcccttca attgttttat ttggagacac tgaaggtagt
    10681 agtttgcgtt gtccaaattg acgaccaatc atataagcac cgatatcagt cgcccagaca
    10741 ataaataaag ctaacaacac cttatctatt ccagccatac gagcactgac tagattttga
    10801 aagccaatac ccacataaaa actagacgca attggaaagg cagcatcttc aaaactgtaa
    10861 gaattactgt tcaaaacagt acctgccaaa atcataaaaa taacaatacc gtatgctgat
    10921 aaactagcgt caactggtaa aaaggacaaa taactatcta aaggaactgt taaaacaaag
    10981 gctcctatca tagctaatgc accttcaaag gaaaatattt ctaaacgacg catacgaagt
    11041 aattcagaaa caccaatcat agctaaaagt ccaactaaaa attggaaagg caaaccaccc
    11101 attactaaaa aagggataaa tatggcaagc gcgacagctc cccaaataac acgttctttc
    11161 attttctctc ttctttctat acactaccaa atcgacgatg gcgttgatta taatccacaa
    11221 tggccttatg tagttcgtct tttttaaaat ctggccataa aacgggagta aagtaaaatt
    11281 cactataagc tgattgccaa ggtaaaaagt tactcaagcg taactctcca ctagtgcgga
    11341 taatcaaatc agggtcccta taaagatagg gtaagctatt tgtcattaaa tgatttgcta
    11401 ctaaatcctc tgtaatatcg tcaggattca acttagcttc caaaacatcc tgagcaattt
    11461 ccttaatggc attagtgatt tctgaacgtc cgccataatt tagcgcaaaa ttcaatacga
    11521 gccccgaatt atgttttgtc ctcaaacaag cgcgttgcat tgcatcgtat gttgccttag
    11581 gtaatttatg agtatctccg atgacctgta cacgtacatt atttttatct aactctggta
    11641 catatttatc aaaaaattca accggaaggt tcataataaa cttaacttca tcatctggac
    11701 gtgaccaatt ttctgtagaa aaggcataga cagttagtac tttaacacct aattctgaag
    11761 ctgctattgt aacttcttga agagcatcca tccccgcctt atgtcccata acgcgtggtt
    11821 ttaaacgttt ttttgcccaa cgtccgttac catccatgat aataccaata tgtgctggta
    11881 ttttctccaa aggcatttct acttgttttt ttcttttaaa tataaacata atgttcctta
    11941 atttagcaat cgtttttctt atcagccctt ttaaaagagc tggaaatatc ccatactata
    12001 acattttacc acattttaac ttatttttga gtattaaaat gccatttgtg acagcaaact
    12061 aaaaagacca gaacaatcat ctggtcctac atatcttatt cagtttcgat ggctgtttct
    12121 ttattttcgt cttcattgac ttcaataatc ttaacatctt caactggttc tgttggtgtt
    12181 gctgccttag agacaacaga tttgatagct gccaactcaa acgtcaagta gacaccatca
    12241 acatctaaaa ccattcgttg tgcttcagta ttaacttcat caactactcc aaaaagacca
    12301 ccaattgtga caatttcatc acctttttga acagcattca attgtttttg acgttcttgt
    12361 gcttgtttct tttgctgacg ttgcatgaag aacatcatac caaccataac aacaagcata
    12421 ataataggta agctcatttg attctccttt ccgtgttcta cgtaatcatt actatagcag
    12481 atttatgcta tatctgcaag gagatactgt ctcttagcca ataaattcac ggatttcttc
    12541 ttttgtcatt tttgaatcgc acttcacctt gatagttccg tttttaacaa ccatcaaacc
    12601 tggcactgta ggaatgtcat atttatttcg aaaatcttta tttgcagtcc aatgatcaaa
    12661 atcttcacta tctaaatgga ataattcaaa ttcatcttca tcagctactt ctacaatttt
    12721 aggaacaaag aggtgacaaa atggacaaga aatacgacca atgaacagta caaaatcttg
    12781 atttgaatca attttatctt caatttcggc agccgttatc aaagtcatgg ttgccaaaag
    12841 ttcttcaaaa gaatacatag ttaaccaact ttctaaatct tatatttaaa gaataaccta
    12901 tgatggcaaa acttgcaagg caagagtaac aacgattcta tcaaaatcat taactcactt
    12961 ctcgcttcaa actatcctca aaaatgctca ctcatattta aatgactcat caaaaaaact
    13021 gagcatagct cagtttttat tttacacctt atttttgtac agcattacta acgcttttag
    13081 caactacatc ggccacatca ttttgaaaag catttggaat gatgtgagtt gtgcttaatt
    13141 cttcctccgg tattaaacta gctatgcctc tcgctgccgc aatttgcatt tcaacagtta
    13201 tcgtttttgc tctagcatct aatgctcctc tgaaaatacc cggaaatgct aaaacattat
    13261 taatttgatt tggaaaatca ctacgaccag ttccaactat gtaagctccg gcttttaagg
    13321 cttgatctgg ataaatttct ggaataggat tcgccattgc aaatacaata ggtttatcag
    13381 ccatcttgga aatccattct gcatgtaatg cttccggagc agaaacacct ataaaaacat
    13441 cggcattctc caaagcatct tctaaggatc cagattggaa ttctctatta gtcaattttg
    13501 caatcgcttt gtgatgaggg gctaatgact cccgatcttt atcgttaata atgccaaatc
    13561 gatcaactac cgttacatgt tttgctcctg ctgatagaag cttccttgtt atagataagc
    13621 ccgctgaacc tccaccatta acaactactt tgatatcttc aatgtctttt ttaactaact
    13681 tcaaactgtt aaacaaagcc gctaatacta cgattgctgt accatgttgg tcatcatgga
    13741 aaactggtat atcacattct tcaattaggc gttgttcaat ttcgaaacaa cgtggtgctg
    13801 aaatatcctc taaattaatc cctccaaaag tcggcgaaat cgcttttact atagaaataa
    13861 tttcctctgt atcatttgtt ttcaacacaa ttggcacagc atctacattt gcaaaacgtt
    13921 taaagagtgc tgctttacct tccatgactg gcattgctgc ctcaggacca atatctccta
    13981 atccaagaac agctgaacca tcacttacta cggctactgt atttttttta gtcgtcaact
    14041 catatgcaag ctccacatcc tctgcaatag ccgatgatac tgacgccacc cctggggtat
    14101 aggcaatact aagatccctc ttatcttcca ccttaacttt tgaaattact tctaattttc
    14161 caccattttc tctagcttgg ttgatagcta attgacctaa attttcactc attttgataa
    14221 ctccttttga taaatattag aataacatgc gtaaaatggc tgtcatcgtg ataacagtaa
    14281 tagctcctcc taaacgcgta gcaacttgag caaatggcat caagttcatt cggtctgctg
    14341 tacttaaaat ggcaacatct cctgttcctc ccataccact ttgacaagct gaaataatac
    14401 ctgcttcaac aggattcata ttaaggaatc gcgatacaaa gaatccaaca gaaataactg
    14461 taaatacaac actaataaca actatgaaat attgtatgct aagcgttgcc acaacatctt
    14521 ttaacgggat ataaagcaat cctagccctg ccattagagg aaatgtaaaa ttaccagaaa
    14581 taaatttata aagttgctta gctccatttt gtgtttctct aggaataaca ttgatatatt
    14641 tcaaaatagc tgctgcgata atcattaata cgggaccagg aaatccagtt aattgctgca
    14701 acaaatgtcc aaaaataaag agactacatg ctgttaaaac acctccaccc attttcttaa
    14761 cgtcaattgg gccagaatga tcagcaataa tatctgacat gtcctctcct ttatttaacc
    14821 ttactaattg cccttgacca gacaagtgtg gtttcttttc tcccaaacga ttcaatagtg
    14881 cagtacataa aatggcaaag aaattaccaa taatagtggc tgggatgagt tgagcaacta
    14941 gttgttcgct agctacaccg gtaattgaac tatagcctaa cgataatggt aaaatacctt
    15001 caccaatacc accagctaaa acgggtgtta cgatataaaa caaagtgtgt cgccactcta
    15061 agcctaaaat aacaccgaca aatgtcccta ccatcatcgc acaaaccatt cctaataaca
    15121 tggggaaaat cattcttagt aatccctgaa tcaacatttt ccgattcatc cctaaaatac
    15181 taccggacac taaacaagca atataaaaat aaagaaagtt agcttgcttc atcaacatat
    15241 ttgttgactc taaaacattt ttattaatga ggttgaaaaa caccaaaata gatggtacta
    15301 ataaagataa aatagctggg ccaccgaagt ttttaaaacc agggatgcta gctccaatag
    15361 tacctaagaa ccatcccatt gttaagatga ctgcaaatcc tcctagcata ttgactggca
    15421 atttttgtaa aaagcctgct agaagaatca ataatgctaa acaaacatat actggtaagg
    15481 gaactgagcc taccttaata tgaaatagtt tagtctttaa gtcttgcttt tgacctctag
    15541 aaccctcatt attcactacc ttcacgtctg ccatgaaagc acctcctttt acttcttatg
    15601 tacagtttac tttgggactt agtgataagg aagtttattt aagtaattga attatttcaa
    15661 cttatttaat taatttaagc tggattctag tttttaggct atgttatact aaactcggag
    15721 gatgctgatg aaaaaaaaac tatctctttg ggcatttcta tcattgattt tagtcactat
    15781 gaccatttgt atattttcta ttttttatta tgtcactata catcagtcct atcgtatggt
    15841 aagggtacag gaagaaaaaa tcttaaaaaa tactggttat gctttatcga gaaatccaca
    15901 agttatacaa acactcaaag ataatcacta taatcaaagt ctccaaaaac aaatgctatt
    15961 ccttagcaaa aaatcaaatt tagactatat tgtccttata aatttaaaag ggatccgttt
    16021 tacccaccct gactccacta agattggtaa accatttcaa ggcggtgatg aacaagctgt
    16081 ttttaaggga aaagctatta tgtctactgc agaaggttct ttaggaaaat ctttacgata
    16141 cttaattcca gtatatgatc accaaaaaca ggttggggca attgcagttg gcttaaaatt
    16201 aacgacactt ggagacttat ctcaaagcag cataaaagag tttagtaagc ctctcttgat
    16261 tagtattttg attagcttag tcgtcacaag tataatttct tatggcttaa aaaaacaact
    16321 tcacaacctc catccaagtg atatttttca acatttagaa gagcgcaatg caacactgga
    16381 ccaaattcaa gccgctgtat ttgttattga ccagaggcat atcatcaaaa gaaataatcc
    16441 tgcagctagt ttattattca aaaaagaggg gcaacgagac ctattttcag gtaaattatt
    16501 agaatcttta attcctcaac tcaagcagga tcatttttca aaaaaaactg aacaagtatt
    16561 acattttcaa ggacaagact atttactatc tatctcgcca attacggtta aaacacaaaa
    16621 tagaggctat gttgtcttct taaggaatgt tactgaaaca cttttcactt tggatcaatt
    16681 agcacacact acagcttatg cttctgctct acaggcccaa acacatcaat ttatgaatca
    16741 acttcatgtt atttatggac ttgcagacat cgaatactac gacgagttga aaatttattt
    16801 aaaagaatta ttggagccac aaaatgaatt tttagcacgc ttatcaatgt tagttagaga
    16861 gcctcgactg gctagtttca ttattggcga aagagaaaaa ttcgcagaaa aacacatcaa
    16921 tctatccaca gaaattttag tagaaatccc taccaagtca actgttgagg atgttaataa
    16981 ctatctcttg ctccacagat atattaatac gaagatactc acgctattaa attcaacgac
    17041 tttagtttct ttacgtttaa attatcaaaa taacctcatt gaaacagatt atcaatggga
    17101 gaatgagaag tggttactca atgactacca tcagtatttc aacgacgctt attttcaaca
//
LOCUS       DATROY010000030        17130 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.030, whole genome shotgun sequence.
ACCESSION   DATROY010000030 DATROY010000000
VERSION     DATROY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            38..694
                     /locus_tag="VBD11_000825"
     CDS             38..694
                     /locus_tag="VBD11_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6953790.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDADNKSQVGQLIGKNPLFSKPTVSRENNHSSQGDSNKQSFSKK
                     VSQVTNVANRPMLTNNSRTISVINKLPKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN
                     "
     gene            902..1978
                     /locus_tag="VBD11_000826"
     CDS             902..1978
                     /locus_tag="VBD11_000826"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6953791.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            2148..2486
                     /locus_tag="VBD11_000827"
     CDS             2148..2486
                     /locus_tag="VBD11_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953792.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2617..4158
                     /locus_tag="VBD11_000828"
     CDS             2617..4158
                     /locus_tag="VBD11_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6953793.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            4198..5430
                     /locus_tag="VBD11_000829"
     CDS             4198..5430
                     /locus_tag="VBD11_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6953794.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5643..6344
                     /gene="pyrF"
                     /locus_tag="VBD11_000830"
     CDS             5643..6344
                     /gene="pyrF"
                     /locus_tag="VBD11_000830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6953795.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            6357..6986
                     /locus_tag="VBD11_000831"
     CDS             6357..6986
                     /locus_tag="VBD11_000831"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6953796.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            6998..8290
                     /locus_tag="VBD11_000832"
     CDS             6998..8290
                     /locus_tag="VBD11_000832"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6953797.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8455..9378
                     /locus_tag="VBD11_000833"
     CDS             8455..9378
                     /locus_tag="VBD11_000833"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6953798.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            9392..10468
                     /gene="carA"
                     /locus_tag="VBD11_000834"
     CDS             9392..10468
                     /gene="carA"
                     /locus_tag="VBD11_000834"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6953799.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10499..13681
                     /gene="carB"
                     /locus_tag="VBD11_000835"
     CDS             10499..13681
                     /gene="carB"
                     /locus_tag="VBD11_000835"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6953800.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            13807..14130
                     /locus_tag="VBD11_000836"
     CDS             13807..14130
                     /locus_tag="VBD11_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953801.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            14127..14597
                     /locus_tag="VBD11_000837"
     CDS             14127..14597
                     /locus_tag="VBD11_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6953802.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            14575..17049
                     /locus_tag="VBD11_000838"
     CDS             14575..17049
                     /locus_tag="VBD11_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO6953803.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHDLVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNFKAVGM
                     GLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILENQLSSYFDNVTTGFIFLI
                     LVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            17058..>17130
                     /locus_tag="VBD11_000839"
     CDS             17058..>17130
                     /locus_tag="VBD11_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO6953804.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSD"
BASE COUNT         5778 a         2751 c         3323 g         5278 t
ORIGIN      
        1 gacttttaaa atttaaaaaa agtaaggaga aaattaattg ttcaataaaa taggttttag
       61 aacttggaaa tcaggaaagc tttggcttta tatgggagtg ctaggatcaa ctattatttt
      121 aggatcaagt cctgtatctg ctatggatag tgttggaaat caaagtcaag gtaatgtttt
      181 agagcgtcgc caacgtgatg cggaaaacaa aagtcagggt aatgttttag agcgtcgcca
      241 acgtgatgcg gaaaacaaga gccaaggcaa tgttttagag cgtcgtcaac gcgatgttga
      301 gaataagagc caaggcaatg ttttagagcg tcgtcaacgt gatgcggaaa acaaaagtca
      361 gggcaatgtt ctagagcgcc gccaacgtga tgcggataac aagagccaag taggtcaact
      421 tatagggaaa aatccacttt tttcaaagcc aactgtatct agagaaaata atcactctag
      481 tcaaggtgac tctaacaaac agtcattctc taaaaaagta tctcaggtta ctaatgtagc
      541 taatagaccg atgttaacta ataattctag aacaatttca gtgataaata aattacctaa
      601 aacaggtggt gatcaaaatg tcatttttaa acttgtaggt tttggtttaa ttttgttaac
      661 aagtcgctgc ggtttgagac gcaatgaaaa ttaagtataa tcaatcattt agtaactatt
      721 ataatgatat atacaatcaa taaaaaggaa tcggatacga gattcctttt tataattagg
      781 ttggttaggg tgactttttt catttggcta ttcttgaaag tttataaaaa tgtagtataa
      841 tagtcacatt aaaatgtttt gaaaatattg atgaataaca tcaacaaata gaggtcatta
      901 tatgggatat accgttgcta tcgtgggtgc tacaggtgcc gtaggaacac aaatgattcg
      961 tcaattagaa caatcgaatt taccaataga acaagtgaaa cttttatcat caagtcgctc
     1021 agcaggtaaa attttacatt ttaaagatga ggctatacgt gttgaagaga caacaaaaga
     1081 atcattttac gatgttgata ttgccttgtt ttcagctggt ggatctattt cagcaaagtt
     1141 cgctccttat gcagtaaagt ctggagcagt tgtagtagat aacacgtcat attttcgtca
     1201 gaatcctgat gttccactag ttgttcctga agtaaatgct catgccatga ttggtcataa
     1261 tggtatcata gcttgtccca attgttctac tattcaaatg atgattgctt tagagcccat
     1321 tcgtcaaaaa tgggggatag agcgtgttat agtttccacc tatcaagctg tttcgggttc
     1381 aggtgcacgt gctgttgaag aaactaagga acagttgaga caagttttaa atgataacct
     1441 ttcacctgat caattaattg caacggtctt gccatgttct agcgatcaaa aacattatcc
     1501 tatcgcattc aatgcattac ctcaaattga tatttttact gataatgatt atacgtacga
     1561 agaaatgaaa atgactttgg agacaaagaa aattatggaa gatgctacta ttaaggtttc
     1621 agctacctgc gtccgcattc ccgttttatc gggtcattct gagtctattt atattgagac
     1681 gaaagaatta gcctcaatct ctgaaattaa aaaggctatt gcaaattttc ctggtgccgt
     1741 cctacaagac cttccatctc agcaaattta ccctcaagcg attaatgctg taggccatag
     1801 ggagaccttt gttggtcgta ttcgaaaaga ccttgatcaa gaaaatggtg ttcatatgtg
     1861 ggtagtttca gataatctac tgaagggggc agcttggaat tctgttcaaa tcgcagaaac
     1921 attgcataag aatgggctgg ttaaaccagc gaaagagtta aaatttgaat taatatagaa
     1981 gaatatgaca ttgcttaata cttatactga cttctagtga tattaatttt tcatcaatat
     2041 caggtaaaac acttgacaaa ttatgaaaac atttgcataa tgaaattaac aaatgaaaat
     2101 gattgcaaat cgttttaaca ttgtttccac attaaggagg ggctcttatg caaatcatca
     2161 aacgtgatgg acagattgtt gaatttgatc ctgaaaaaat ctaccaagct atcttgaaag
     2221 ctgctcaaac agtttatgtg cttgatgagt cacttcgtca agaccttgct caagtgacta
     2281 aaaaagtaat cttagattta gaggatgctc aggttgaacg accaacgatc aatatgattc
     2341 agtctttggt tgaaaatcgt ttattggatg caggatacat tcatattgct gaacactata
     2401 tttcataccg tttgcaacgt gatttagagc gaaatggtta tgatgatcaa atcactgttc
     2461 atttacactt tgaaaaaatt aagtaaggtt tgacttttaa gtttaaactc tctatacttg
     2521 tatatggaac ttactatgta atgtgctaat ttaaaaactt gtagagtgtc tctatgagtt
     2581 ttttaataac ctctattatc aatcaaagga ttttaaatga gtattttaga agtaaaaaac
     2641 ttaagtcacg gttttggtga tcgtgctatt tttgagaatg tttcttttcg ccttttaaaa
     2701 ggtgaacata taggacttat tggtgctaat ggtgaaggaa aatcaacatt catgtcaatt
     2761 gttacaggtc atcttcaacc tgatgaagga aaaattgaat ggtcaaaata tgtaactgct
     2821 ggttaccttg atcaacatac tgtgttagaa gaaggtcaaa cagttcgtga tgttttgcga
     2881 acagcatttg atgaactttt taaaacagag gcacgtatca atgatatata tatatccatg
     2941 gcagatgatg gcgcagatgt agatagcctt atggaagaag ttggagaact acaagatcgt
     3001 ttagaatcac gtgattttta tacgcttgat gctaaaattg atgaagtcgc acgggcatta
     3061 ggagttatgg attttggtat ggattctgat gttactgaat tatctggtgg tcaaaggaca
     3121 aaggtccttt tagcaaaact gttattagaa aagccagata tcctcctgct tgatgagcct
     3181 acaaactacc ttgatgctga acacattgat tggttaaaac gctatttaca aaattatgaa
     3241 aatgcttttg ttttaatctc acacgatatt ccatttttga acgatgttat caatattgtc
     3301 tatcatgttg aaaatcaaga tttagtaaga tattccggtg attatactaa ttttgaatca
     3361 gtctatgcaa tgaaaaaggc tcaattagaa gcagcctatg aacgtcaaca aaaggaaata
     3421 gctgatttac aagattttgt taatcgtaat aaagcacgcg ttgcaacacg taatatggca
     3481 atgtctcgtc aaaagaaact agacaaaatg gatattattg agttacaagc agagaaacca
     3541 aagccaagtt ttgaatttaa agaatctcgc acaccaggac gttttatttt ccaagctaaa
     3601 gatcttcaaa ttggatatga tcgtgcttta acaaaacctc taaacctaac atttgaacgt
     3661 aatcaaaaaa tagctattgt tggagcaaat gggataggaa aaacaacatt acttaagagt
     3721 ttactaggta ttatcccccc tatttctggt aatgttgaac gaggtgattt tattgattta
     3781 ggttattttg aacaagaagt acccggaggg aatcgccaaa ctccacttga agcagtatgg
     3841 gatgcttttc ctgctttgaa ccaagcagag gttagggcag ctcttgctcg ttgtggttta
     3901 acttcaaagc atattgaaag tcagattcag gtgttatctg gtggggagca atcaaaggtc
     3961 agattttgct tattaatgaa tcgcgaaaat aatgtccttg tacttgatga accaaccaat
     4021 catttagatg tagatgctaa agatgagtta aaacgtgctt taaaagctta taagggatca
     4081 atcttgatgg tatgtcatga accggacttt tatgaaggat ggatggatga tgtttgggac
     4141 tttaaccaac ttagttaagt ggctagggta ataaagtaga aggcagagcc aaataagatg
     4201 caaaatgata tgagcgttgt tcttagagct ggtaaattac tgattgaaag cggagcagaa
     4261 gtctatcgtg tcgaagacac aatgaagcat tttgctaagg ctcttcagat agaaaatttt
     4321 gaagcttatg tggttagtag cagtattatc gcttccggga ttaaccgcta tggtaaacaa
     4381 gaagctaaag tatgcaatac agatggtgta acagcaaatt taggtagact tgaagctgtt
     4441 aataaccttt ctaggcaaat tgctaaacaa gacttagtat ctccagaaga aattgtaaag
     4501 cagttagact taattgaaca tcaaaaagat tatagtttgc ttgttacttt aatttcttat
     4561 ttttgtggag cgggtagttt ttctttagcc ttgggtagtt ccttattaga ttcattttcc
     4621 gctgctgtaa caggcttaat tttaggatat tttttgaatt tgatggaaag tcgtatacat
     4681 acaggatttc ttttgaccat tctaggtagt tctgtagtgg ctttaagtgc taatttatta
     4741 tatttttcag gattaggtga gcatcgtagc attatcatat taggggcatt aatggttatg
     4801 gtgccagggg ctgcctttgt taattcggtg agagaatttt cacagaataa tttttcaaca
     4861 ggtttagctt tgattatgtc tgctttactc atttgtattt ctatatcagc aggcgtggct
     4921 attacaattg aaattattcc gtttgcagaa caaatgacag gttctttttc tggtgttcct
     4981 aataccattc ttgagattat catcagaaca ctaatggccg gtttagggac gatagccttc
     5041 tctattctct accatgttcc aaaacgttat ttccttgact tagggatact aggagcaata
     5101 tcttggatgc tctacctgat cttatggcaa caatttcaca tggatgctat agccgtattt
     5161 tttccagggc ttttcatcac ttatttttca cggctattag ctgctaaacg taagtgtcct
     5221 gccactgtct ttttagcgac aagtatgttt cctcttatcc caggacttag cttctataga
     5281 gccgtttact ttttattgac gggagcagat gccttagcaa tggaatattt tagatcctgt
     5341 tttgtaacag cttttacaat tgctatagca atcagtattg tacagcagat tcctctatcg
     5401 ttctttatta gacgtaaaat gataaaataa tttagtattt atttgacaaa atgattgtaa
     5461 acttgtagaa tacctacaaa tgtatcttta agtgagtcca gagaggcgaa caaggtcacc
     5521 atcataagtc aaatagcgag tgctttttcg ctaacttttt atgaaacctt gccctcaggc
     5581 aaggtttttt gtgtcatact ggtacatttc caacaattat tatttctata gagaggaatt
     5641 atatgttaga aaaatgccct atcattgcct tagatttttc tgatttggct tctgtaacaa
     5701 cttttttaga acattttcca aaagaagaat tgctttttgt caaaatagga atggagcttt
     5761 attattccga aggtccctca attattagat atattaaatc cttaggacat cgcatttttt
     5821 tggatttaaa attacatgat atccctaata cagttcgatt atctatgtct gtcttagcca
     5881 aattaggcat tgatatgact aatgtccatg ctgcaggagg agtcgaaatg atgaaagcag
     5941 ctagagaagg tttgggggag ggtccaatac tattagcagt gacacaatta acctcgactt
     6001 cacaagaaca aatgcaggtg gatcaacata ttaacttaag tgtagttgat tcggtttgcc
     6061 attatgctca aaaagctcaa gaagcaggct tagatggagt agtcgcatcg gctcaagagg
     6121 taaagcaaat caagaaacag actaacgaac atttcatttg cttaacgcct ggtatcagac
     6181 ctccacagac caatcagtta gatgatcaaa aaagaacgat gactcctgag caagcacgta
     6241 tagttggggc tgattatatc gttgtgggaa gacctataac gaaagcagaa aatccatatc
     6301 aagcatatct agacataaaa gaagaatgga atcgtattaa ataaaggagt actgagatgg
     6361 atttagcaag acagattgcc atggaactat tagatattca agctgtttac ttaagacccc
     6421 aacaaccctt tacttgggct tcgggggtca aatcgccaat ttacactgat aatcgtgtaa
     6481 ccttatcgta tcctgagaca aggacactga ttgaaaatgg ttttgttaaa caaatacaga
     6541 aacatttccc aaacgttgat atcatcgcag gaacagcgac tgcaggcata ccacatggcg
     6601 ctattatagc tgataagatg aacttgccct ttgcctatat acgctcgaag gcaaaggatc
     6661 atggtgtggg gaatcaaatc gagggacgtg tttactcagg ccaaaaaatg gttatcattg
     6721 aagatttgat ttcaacagga ggatctgttt tagaggctgt tacggctgcg cagagtcaag
     6781 gtattgaggt tttaggggtg gtagcgatat ttacatatca attagctaag gctgaacaag
     6841 catttcgtga agctgatatt cctttggtga ctttgacgga ttacaatcaa ttaattaaag
     6901 ttgctaaagt taatggatat attacagctg accaactggt attactgaaa aaatttaaag
     6961 aagatcaaat gaattggcaa agttagagag gacagatatg tatatcatta aaaacggatt
     7021 gattatcgac cctcaaagtg gttttaatca agtatcagac atgctgattg atcaaggaaa
     7081 aattaaacaa atatcgaaag aaattgatat taaaggcatt ccaatcattg atgcaagcaa
     7141 taaaattgta gctccaggat tagtcgatat tcatgtacat tttcgtgagc ctggacagac
     7201 acataaagaa aatatccata caggagcctt atcagctgca gcaggtggtt ttacgacagt
     7261 tcttatgatg gccaatacta acccaacgat ttctagctca gaaattgtaa aacaagttaa
     7321 ggagagtgca gcaaaagaag caattaagat tgaaacagtt gctacaatta ctaaatccct
     7381 gaatggtaaa gatttagtta atttcgaaga attattagaa gctggagttg ctggattctc
     7441 agacgatggt atccctttga cagatactaa ggttttgcaa gaagctatga acttagctag
     7501 aaaacatgat gttgtcctat cgttacatga agaggatcca tcgttaaatg gtgttcttgg
     7561 tatcaacgaa catattgctc agaaaattta tcatgtctgt ggagctagtg gtcttgctga
     7621 atattcaatg attgcgcgag atgctatgat tgcttaccaa acacaggcga aagtccatat
     7681 ccagcatttg tcaagttcag aatcagtaga agtagttgat tttgcgcaga agcttggtgc
     7741 taacttaaca gcagaagtaa ccccccaaca tttttcaaag acagaggact tacttttaac
     7801 taagggtgct aatgcaaaat taaatccacc actgcgactt gaaaaggata gacaagccct
     7861 cattgatggt cttaaaagtg gtgttatttc aattattgca agtgatcacg ctccccatca
     7921 tattatggag aaagcagcag ataatattag tcaagcgccc tctggtatga ctggcttaga
     7981 gacatcatta gccttaggga ttacttattt agtatctact aaagaactct caatgattga
     8041 ctttttggca aaaatgacat gtaatcctgc acagttatat ggatttgatg caggttatct
     8101 cagagaaggt ggacctgcag atatcgttat ttttgatcaa gctgaagaga gaataataaa
     8161 ggcagaattt gcctccaaat ccagcaactc tccttttatc ggtgataaat tgaagggagt
     8221 tgttcactac acaatttgca atggtgaaat agtctatcaa aaagatagcc acgcagcgac
     8281 attggtatag gtaaaacatt tcttattaat atcctttaag gtattgtcca gagaggcaaa
     8341 caaggaattg cgttaacaaa tagtaagtta gttccctatc tagcttacta ttttgcagag
     8401 cctccttgtt ttaggaggct tttttctagc taaaatttat agaaagaaat tatcatgaat
     8461 catcaaacac aaacactttc attagaacat tttgtcagtt tagaagaatt atcaaatcaa
     8521 gaagttatgt ctttgataaa gagaagtatc gaagttaaag aaaatcctag caacattggt
     8581 tttgataaag attactatgt gtcaaatctc ttttttgaga attcaacaag aacacataag
     8641 tcatttgaaa tggcagaact caaattagga ttaaaaacaa ttgaatttaa tgctgataca
     8701 agttctgtta ataaaggaga aaccctttat gacactatct taacaatgag tgctctagga
     8761 ttggacgttt gtgtcattag gcaccctgat attgactatt ataaagaatt gattgctagt
     8821 ccgaacattc attcagctat tgtcaatggg ggtgatggct caggccaaca tccaagccaa
     8881 tcacttttgg atttggtaac tatttacgaa gaatttggat acttcaaagg actaaaaatt
     8941 gcgattgtag gagatctaac tcactcacgg gtagctaaat ctaacatgca agtgctaaaa
     9001 cgattaggag ctgaaatttt cttttctgga cccaaagaat ggtatagtag tcagttcgat
     9061 gaatatgggc aatgtttacc aatagatcag ttagttgatc aaattgatgt ccttatgctt
     9121 ttacgtgttc aacacgaacg tcatgatggt aaaggggtgt tttcaaaaga aagctatcat
     9181 caacaatttg gtcttacaaa agaacgctat aaacatttaa gggatacagc aattattatg
     9241 cacccagcac ccgtcaatag ggatgtagag attgctagcg atttagtaga agcagataag
     9301 gctcgaatag tgaaacagat gagtaatggc gtctatgcaa ggatagctat cttagaagca
     9361 gtgctcaata gcagataata aaggagataa aatgaagcgt ctattattat tggaagatgg
     9421 tagtgtgttt gagggtgagg ctttcggagc ggatgtagga acaagtggtg aaattgtttt
     9481 tagtacagga atgacagggt atcaagaatc tataacagat caatcttaca acggtcaaat
     9541 cattactttt acttatcctc ttattggaaa ttatgggata aatcgagacg attacgaatc
     9601 tattagacca acatgtaagg gagtaattgt ttatgaatgg gcagagtatc caagtaactg
     9661 gcgtcaacag atgacatttg atgaattttt aaaattaaaa ggaatacctg gaatttcagg
     9721 tatagatact cgagcattaa caaaaattat tcgaaaacat ggaacaatga aagcttgttt
     9781 aataaacgag ggaaattcta ttcatgaagc actagaaaac cttcaaaaaa gtgttctttt
     9841 aaataatcaa attgaacaag tatcaaccaa attagcttat gcttcgcctg gagttggcaa
     9901 aaatattgta ttagttgact ttggtttaaa acattctatt ctaagagaac tgtcacaacg
     9961 tcaatgtcat attacagtcg tccctcatac tacgacagcg caagaaatct taaatctcaa
    10021 tccagatgga gtactcttat ctaacggccc tgggaaccca gaacaattac cgaatgcttt
    10081 acaaatgatc caagaaattc aaggtaaaat tccaattttt ggtatttgta tgggacatca
    10141 actatttgct aaagccaatg gtgcaaagac ttataaaatg acttttggtc atcgaggttt
    10201 taaccatgct gttcgtcatt tgcaaacagg acaggttgat tttacaagtc aaaatcatgg
    10261 ttatgctgtc tcgagagaag atttccctga ggctctcttc attacacatg aagagattaa
    10321 tgataaaacc gtcgaaggtg ttcgacataa atactatcct gccttttcag tgcagtttca
    10381 cccagatgca gcaccagggc cgcatgatac atcttatctt tttgatgaat ttattaatat
    10441 gattgatgat ttccagcaaa aaagctagct ttaaaatata gaattagagg agtttgatat
    10501 gccaaagcgc acagacatac gtaaaattat ggttatcggt tctggcccga ttgtgattgg
    10561 acaagcagca gagtttgact attctggtac acaagcctgt ctctctttaa aagaggaggg
    10621 ataccaagtt gttttggtca attcgaatcc ggctactatc atgacagata aagatattgc
    10681 tgataaagtt tatattgaac ctattacctt ggagtttgtc acaagaattc tcaggaagga
    10741 aagacctgat gctctccttc caactcttgg aggtcaaact ggattgaata tggctatggc
    10801 tctatcaaaa aatggtatcc tagaagaatt gaatgttgaa ctattaggga ccaaattatc
    10861 agccattgat aaagctgaag atcgtgattt atttaaacag ttgatggaag aactcaatca
    10921 acctattcca gaatccgaga ttgttaattc ggtagaagaa gctattcaat ttgcagagca
    10981 aatcgggtat ccattaattg ttcgtcctgc ctttacttta ggaggtacag gtggaggcat
    11041 gtgcgataat caagaacaat tggttgacat cacgacgaag gggttaaagt tatcgcctgt
    11101 gacacagtgc cttattgaac gttcaattgc tggctttaaa gagattgaat acgaagttat
    11161 gcgtgatgca gcagataacg ctcttgttgt ttgtaatatg gagaattttg atcctgttgg
    11221 tatccataca ggggactcta tagtttttgc acccgcacaa acgttatctg atgtggaaaa
    11281 tcagttatta cgagatgcga gtttagatat tataagagct ttgaaaattg aaggaggatg
    11341 caatgttcaa ctagcccttg atccgaatag ttttaaatac tatgtcattg aagttaatcc
    11401 aagagtatca agatcttctg ctttagcttc aaaagctact ggatatccaa ttgctaaatt
    11461 agcagctaag atagctgttg gtttgacact agatgaggta attaatccta ttacaaaaac
    11521 gacttacgct atgtttgaac ctgctttaga ttacgtagtc gcaaaaatgc cgagatttcc
    11581 ttttgataaa tttgaaagtg gcgatagaaa attgggaaca cagatgaaag ctacaggcga
    11641 agtcatggca ataggacgga atatagaaga atctttgttg aaagcttgtc gttcactaga
    11701 aataggtgtc gatcacatta aaatagcaga tttagataat gtatcagatg atgttttatt
    11761 agaaaaaata agaaaggcag aagatgatcg cttattttat ctagcagaag ctttacgtag
    11821 acattatagt attgaaaaat tagcaagttt gactagcatt gattctttct tccttgataa
    11881 gttaagagtg attgtagagt tagaagatct attatctaaa aacagactgg atatcaatat
    11941 tctcaaaaag gttaaaaata aaggtttttc agataaagct atagcgagtt tatggcaaat
    12001 aaacgaagat caagttcgta acatgcgaaa agaagcagga attcttccag tttataagat
    12061 ggttgataca tgtgcggctg agtttgattc agcaacgccc tacttttatt cgacttatgc
    12121 cgtagaaaac gagtcattga tatcagataa agcctctatt ttggttttag gatcgggccc
    12181 aatccgaatt ggacagggag ttgaatttga ttattcaacg gttcattctg ttaaagccat
    12241 cagagagtct ggttttgaag cgattatcat gaactctaat ccagagacgg tctcaacgga
    12301 tttctctatt tcagataagc tttactttga acctttgact tttgaagatg ttatgaatgt
    12361 tattgactta gaaaaacctg aaggggtcat cttacaattc ggtggtcaga ctgctattaa
    12421 tctagcaaaa gatttaaaca aggcaggggt taaaatacta ggcacacaat tagaagattt
    12481 ggatcgtgct gaaaatagaa aacaatttga agccacttta caagctctaa atatccctca
    12541 accaccaggt tttaccgcca ctacggagga agaggcagtt aatgcagcgc aaaagattgg
    12601 ttatcccgtg ttggttagac catcatatgt tcttggtgga cgagctatga agatagttga
    12661 aaacgaagaa gatttacgtc attatatgac aaccgcggta aaggcaagcc cagatcatcc
    12721 agtgcttata gatgcatacc ttataggtaa agagtgtgag gttgatgcta tatccgatgg
    12781 tcagaatata ctgattcctg gaataatgga acatatagag cgagctggtg ttcattcagg
    12841 agattccatg gctgtatacc ctcctcaaac attgtctgag accattatcg aaactattgt
    12901 agattataca aaacgcttag caattggatt aaactgtatc ggtatgatga atattcagtt
    12961 tgtaatcaag gatcagaaag tatatgttat tgaagttaat cctcgtgctg gtagaacact
    13021 tccattctta tcaaaagtaa ctcacattcc tatggcccaa gtagcaacaa aagttatctt
    13081 aggagataaa ctctgcaatt ttacatatgg ttatgatctc tatccagctt cagatatggt
    13141 ccacattaaa gcccctgtct ttagttttac caaacttgct aaagtagata gtttattagg
    13201 tcctgaaatg aaatcaacag gcgaagtgat gggatcagat attaatcttc agaaagcctt
    13261 gtataaagcc tttgaagcag catatctcca tatgccagac tatggtaaca ttgtttttac
    13321 agtagatgat acagacaaag aagaagcttt agaacttgca aaagtctatc aaagtattgg
    13381 ttatcgtatt tatgctaccc aagggactgc aatttatttt gatgctaatg gtttagagac
    13441 agttttagta ggtaagttag gagagaatga tcgaaatcat attcctgatt taattaaaaa
    13501 tggtaagatt caagcagtta tcaacacagt tgggcaaaat aacattgata accatgatgc
    13561 tctcatcatc agacgttctg caattgaaca aggagttcct ctatttacat ctttagatac
    13621 tgcacatgca atgttcaaag ttcttgaaag caaagcattt acattaaaag tactagatta
    13681 atcaaacaat caaggtaaaa ttaacttcca gcaaaacaga atcggactag attaaatata
    13741 gtttgttctg tttttataaa aaagcctaat tgtgataaaa tagggaacaa acttaaggag
    13801 ttaattatgt taaaaacacg aacacatatt aatgaattag aaacaaaaat cgcccttgat
    13861 ttagtacaca gatatctagg tattcaacaa cttgtttttt caaagtggag acattcttta
    13921 gaaactggtt catggtcttc tattataaca ataaatggcg ctaatattat caattacatt
    13981 gttgatgata ttagcgacag tgatagtatt tcaggtatta tttgtcatcc taaaagtttt
    14041 catctcagac ctagagaaaa aaaggaacct attcagtcta ttgatgagtt agatatcatc
    14101 tggtctttga atagagagga aaatgaatga ttccagatag tagttttaaa gtgctatcag
    14161 atatggcata taatattgaa aaatccaaag cagaaagtaa tggacacctt aaaattatag
    14221 aaggtagtca aattattgat ttccatcaaa aacgctcttt tatggtatta gaggttaatg
    14281 ataatatcga taatggtatg caggctatgg catgtcagtc tttggatgag agtaatcata
    14341 ttgttatagc atattcagga acgaacgctt ttaataagaa agatatctta acagatatcg
    14401 attcagttgt attggggaag aacaataaat tgctaacttg tccttcaagt attaccctga
    14461 gagaggatgg catttctctg ccttgtaaaa atccaagtca aattacaaca tctaaactat
    14521 ttgcacaaaa aattaagatg aactatcctg atgcaaccct ctggttaaca ggccatggta
    14581 aatgttcagt caggtaatat ctcaaattac caaaaaaatc ttttagattc tgcaactaat
    14641 ttccaaaata tctttccagc cctcgttaac cagtatagta gttccattac tgctaacgaa
    14701 tcactgaaaa aatctttaga agcttctgat aacatgttta atgatttgat gacaacccag
    14761 acaaatactg gaaaagattt atcaagctta atagaacagc gccatcaaga tagcatttcg
    14821 tatgaagcgt tttcggcctc attactagaa atgaataacg agttattaga gaagcaatta
    14881 tctgatatta tcacacaagc acaaaaagac caagaaacat tatcatcaca actcaatagt
    14941 attatgggtg atgataacga taatgatcat aatcataaag aaaactcatc agcttatcta
    15001 aatgttgcaa ggcaaaaaat ccaagaacta tctgaagcac tcaagtcaca agataacatc
    15061 gctaaagatc aaagtgaaca actagataaa attgttagag agggactagc aagttacttt
    15121 gctaagaata ataaagataa tattacttta ttagaattat tgaagagtca ttctactaat
    15181 gagaagactt tgaaagattt taaagttaag gtagcagatt tcacaaattc tctcatttca
    15241 agcattccct cactaaacct ttcggagttg cacttaaccc aagaagaaaa agccattcag
    15301 tttacatctt ctgattcgga aatcattaaa aaagtaagca atgaaagaac tctgtattta
    15361 aatactaatt tattgaatcg tctttttgaa gctaggaaaa atagagatga agccaaaaat
    15421 aaagttaatc aattaaaatt gccatctagt tctactcgaa ctggtgagca gatagtttct
    15481 gttgaatcaa ataatccaga ttacagggta gatacctgga cggttaatgg taagcaaaca
    15541 agaaccttgg accccagcca aaacaataat attattatca atagcagtta tcaaggggaa
    15601 ttatctaact ctagtattac aaaaccaagc tatacaatca ctatcgggaa tcagaaacaa
    15661 attgtacaaa atgatgacgg tgaaattcaa cggtcttact tggaggcaga agcaacgtat
    15721 caacgaactc tacaagaagt aattgatgca tataacacca ctcacgactt agtagctaaa
    15781 tactacatta tttctgatgg cgaagaaccg caaaatttat ttgatcaatt tttaaatcaa
    15841 agtgttaacg atacgatggt tgatctggtc aaaaatggta taactaagta tttgatggat
    15901 gaaaacactg ctgatgcgca acagaaagtt aaagatgtca tggaggaagt tgaaaatagc
    15961 caagatgaat tggcggatca aatggcaaaa gtaactgaga caaatgtacg actaacagat
    16021 acaatcaaaa aacagcttga gactcttcaa acaattaaca tgaaggtcca aaacatcaca
    16081 caggatcaat ctaaagtaaa cgactcacag aaaacaactg atcaacaatt atctgatttg
    16141 aagaaccagc tagatgggct aatgacctct gcaactggcg taaaagatat gtccaaatct
    16201 aatagtcaag aggcggacca agttaatcaa atattcacat cattcaataa agatgttcaa
    16261 gatgctaaaa attcgggcaa caaactttcg acggatgcaa cagatttaat ggctaacttc
    16321 caaaaagaat tggccaataa cggtgatttc gtagcttctt tctctaaagt ttttaatact
    16381 gcttataaaa atggagtgcc aaatgatatt ctccttaatt tcttatcacg acctgtcgct
    16441 gaatcagcgt ctgcagttag ggcaactgaa aatacttatc gcccattcac ttggatacta
    16501 ttattagaag ttgttagcct atttatagca tatatctttg cgactcaaaa ccttattaag
    16561 aaattgacag ataggtataa tgtcaatcgc tggcttcaaa cagacttttt aaatatcatt
    16621 gtcatttcag gcctctctct tgtgattggt ttagcactgg gagttatctc aagcagaagt
    16681 cttcatgtca tgcctgaata tgtaccatct tggttcctag ttatgactct gtttagtttt
    16741 ttattaattc atagtcagta tttctttatt aaaaatttta aagcagttgg tatgggttta
    16801 gctttgttta tgattattag ttttgtgtat ctatcaaatg ccgtaggcac agtagcgact
    16861 gttagtggat ttccaaaatt actaaaagct attaatccac tatctatcct tgaaaatcaa
    16921 ttatcatctt attttgataa tgtaacaact ggatttattt tccttatctt agtacttctt
    16981 gtggatgttg cttttattat catgaatatt tttatcacct taaattttga agctaaagta
    17041 aaagagtgag gttgttgatg aagttgaaac gatttttagc ctgtctacta atattatcct
    17101 gtatgagtca aacatcagta ttttctgatg
//
LOCUS       DATROY010000031        16861 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.031, whole genome shotgun sequence.
ACCESSION   DATROY010000031 DATROY010000000
VERSION     DATROY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16861
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..807)
                     /locus_tag="VBD11_000840"
     CDS             complement(<1..807)
                     /locus_tag="VBD11_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6953805.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKN"
     gene            complement(831..1625)
                     /locus_tag="VBD11_000841"
     CDS             complement(831..1625)
                     /locus_tag="VBD11_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953806.1"
                     /translation="MSHIKSENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(1784..3235)
                     /locus_tag="VBD11_000842"
     CDS             complement(1784..3235)
                     /locus_tag="VBD11_000842"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6953807.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            3359..4993
                     /locus_tag="VBD11_000843"
     CDS             3359..4993
                     /locus_tag="VBD11_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6953808.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            5128..6348
                     /gene="pepT"
                     /locus_tag="VBD11_000844"
     CDS             5128..6348
                     /gene="pepT"
                     /locus_tag="VBD11_000844"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6953809.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            6377..6868
                     /locus_tag="VBD11_000845"
     CDS             6377..6868
                     /locus_tag="VBD11_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6953810.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(6855..7052)
                     /locus_tag="VBD11_000846"
     CDS             complement(6855..7052)
                     /locus_tag="VBD11_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6953811.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            7098..7622
                     /locus_tag="VBD11_000847"
     CDS             7098..7622
                     /locus_tag="VBD11_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6953812.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            7633..8316
                     /locus_tag="VBD11_000848"
     CDS             7633..8316
                     /locus_tag="VBD11_000848"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6953813.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    8337..8462
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8477..9007
                     /gene="infC"
                     /locus_tag="VBD11_000849"
     CDS             8477..9007
                     /gene="infC"
                     /locus_tag="VBD11_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6953814.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            9047..9247
                     /gene="rpmI"
                     /locus_tag="VBD11_000850"
     CDS             9047..9247
                     /gene="rpmI"
                     /locus_tag="VBD11_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6953815.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            9305..9664
                     /gene="rplT"
                     /locus_tag="VBD11_000851"
     CDS             9305..9664
                     /gene="rplT"
                     /locus_tag="VBD11_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6953816.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(9907..12051)
                     /locus_tag="VBD11_000852"
     CDS             complement(9907..12051)
                     /locus_tag="VBD11_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6953817.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            12186..13343
                     /locus_tag="VBD11_000853"
     CDS             12186..13343
                     /locus_tag="VBD11_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6953818.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            13343..14020
                     /locus_tag="VBD11_000854"
     CDS             13343..14020
                     /locus_tag="VBD11_000854"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6953819.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            14114..15181
                     /locus_tag="VBD11_000855"
     CDS             14114..15181
                     /locus_tag="VBD11_000855"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6953820.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            15182..16348
                     /gene="aroC"
                     /locus_tag="VBD11_000856"
     CDS             15182..16348
                     /gene="aroC"
                     /locus_tag="VBD11_000856"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6953821.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            16432..16767
                     /locus_tag="VBD11_000857"
     CDS             16432..16767
                     /locus_tag="VBD11_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6953822.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         5432 a         2662 c         3242 g         5525 t
ORIGIN      
        1 attcttccag acatcacttt ctttaagtga tgaagctgct tttttagtcg ttttgtttat
       61 attaacaagg gcataatctc caacgtaatc accgattgct tcttgcgaaa cggtaaacca
      121 ccctttttta aagacatctt ttttgacttt ttctggggca gcataaccta gtgaatcata
      181 gattagttct ccaccgcgtc caaaattatt accatataaa tagatatttt tatcataaaa
      241 atccataata gtaaaagtag tgttaggctt taagatatgg tgtaaatctt ttttggcagc
      301 gagagtttta gttttccatt ggctaaccca ctgattagct tctttttctt taccgaatac
      361 tttccccaag gctggcatca tatctaaata attttgtgca ccatatttaa taactaaagt
      421 tggtgcaatt tttttcagag tattgatgtt tggatcttga tcgaaaacca tgattaaatc
      481 aggtttttgt gcggcaatag cttctgtatc atcagcagtt aattttttag cttctttcag
      541 ttgcttacca aaaacggggc tatctttttc taagtctaaa ctgtaacttg aaacattaac
      601 acctagtttt aataaatacc cagtgtaaga atatgtaaaa ttaattactt ttttcggatt
      661 ttcaggaatt tttccataat aggtgaagcc ttcaatttta ggcattttag aaatagttgt
      721 tttagtgctt tcttctttag tttgttgtct gcaagatacc aaaaagaagg tcagtagtgt
      781 gaggacaata attccaattt ttttcataag ataacatctc ctaaataata ttaattttga
      841 tcagttgata attgataaga taacaaaaca ggttgtttac taatagggtc ctgaatgata
      901 tgagcatcaa tcttaaaaat atctttgata atatctttag tcatgatctg actaggacta
      961 ccttcataga taattttacc ggttttcatt gcaactagat aatcagaata gcgcgcagac
     1021 aggtttaggt cgtgtaggac cataataatt gttttttgcg tttcgttatt aagttttttg
     1081 agcaattcta agacttctaa ttgatgattc atgtcaagat aagttgtagg ttcgtctaaa
     1141 aagatagtat ctgtatcttg tgctaaagcc atagcaatcc atactttttg cctctgacct
     1201 cctgaaaggt catcaacatc tctattagca aattgcgcta cgttagtcat ttccatcgcc
     1261 caatggattt tactacgatc atcatttgtc aaattgccaa ggtatttttg gtgtgggaac
     1321 cgtccataag atattagttc ataaactgtt atcccttcag ttgcttctaa cacctgtggg
     1381 agaagtgcaa tcttcttagc aatttccttt gtgggtaaat gtgcaatatc atgaccatca
     1441 atagtgatag taccatcttt tattttatgg atccgtgtga gagccttcaa aagagtagac
     1501 tttccacatc cattggcacc aataatcgtt gttatttttt ggttaagtat actgagggaa
     1561 agattattga tgatttcttt ctgatcgtaa gatacaataa tgttttctga ttttatgtga
     1621 gacatagaga acctttctag caaaaaatta agttcattat aacatagaaa aaaggcttac
     1681 aaaagccttt tttagcatta attaagaaaa atgtgaacga aatgataatt atttagaatg
     1741 attcaaaata gtatcaataa tcattattgt ttagcttttt ttatcattta aggtattttt
     1801 tcgcaacttc taaatctcca gagtaattgt cacgatttcc tttgataatt tgataagcat
     1861 ctgccccttt accagcgata ataattgcat ctagtttgca attagtaaga gtactggcat
     1921 tagcaattgc ttcttctcga tcaatgatga tagtaaccgg acgattaata tggcttgcga
     1981 tttcttgtga gataactaag ggatcttcaa aattaggatc atctgcagtg agtatcactt
     2041 gtagattagg atgttgatta atgacatctc cgaagtcttt acgacggctt tgacctttat
     2101 taccaggagc tccgagcact aatataatat cccctttttg gtgttcctca acaacagcta
     2161 ataatttctt caaactgtct ccgttgtggg catagtcaac gaaaatcttt gcgccgttgg
     2221 tttgtgttag gacttccatg cggccaggga cggtagtttg tgcaattccg ttttttatat
     2281 cttcaattga aacaccaagt cggagacaag caagtccagc tgcgagagca ttttcttgat
     2341 tgaattttcc aatcaggttg atattgtagg tgttctccaa ctttcctttg acatggaaag
     2401 aaaaggcctg tgattctgtg ataacatttt cagaataatc tccatagaaa tcatgaggaa
     2461 tatattctac ctgttctttg acaatattaa aatgatccat ctgactattt acaacgacag
     2521 cattgctatt ctccatcaag agccgtttgt ggaaaaaata atcttcgaat gtagggtgct
     2581 cgataggacc aatatgatcg ggactaatat ttaagaaaac gccaacgtca aaggttaaac
     2641 cataaacacg tttggttagg tatgcttggc ttgatacttc catgactaag tgtgtcattt
     2701 gattctcaac ggctgtagcc atcatccgaa ataaatcaag actctcgggg gttgtgagat
     2761 gtgatttaaa gaaagattta ccatctaatg ttgtgttcat agttgagagc atagcaggac
     2821 gatggtttac ttttagcata tgataagcaa agtaagccgc agttgttttt ccttttgttc
     2881 cagtaaaagc tagaagttta agtttgtttt gtgggttatt atagaaagac atagatatca
     2941 aactcatggc ttttttgata tcattgacta atatcactgg aatatcggcg ccgtaatcta
     3001 tctcagaaac gtaaaaagaa aggcctgctg tgattgcaga atctagatat tcttttttaa
     3061 aagtggcacc cttagcaaaa aataaagtat cagatgaaat ttgacgacta tcatatgata
     3121 aggcattaaa agttacattt tgtgtccagt tataataata atgctcatga aacaaaatct
     3181 ctctgaaatt atggtcattt tttaaaattt ctaaaatttt atctattgta atcatactcc
     3241 ttattttaga ctttatgttc tcgttttaca agtctaaggt aaaagtttat aataaagtga
     3301 tatttctgag atttttgtct tttaaatgct aaataatatt gaaaatagaa agtttaccat
     3361 gtcacaaaaa acaacaaaag ttagtcagca agaacaaatg gtcaaaggga cggcttggtt
     3421 aacagcaggg aatttcatta gtcgcttgct gggagccatc tatattatcc cttggtatgt
     3481 ttggatggga aagcatgctg ccgaagcaaa tgctttattt gggatgggat atgaaatcta
     3541 tgctttattt cttttgatat caactgttgg aattccagta gcagttgcaa aacaagtatc
     3601 taagtataac actttaggta aagaagagat gagcatttat ctggttcgga aaatcttgca
     3661 gtttatgctg attttaggtg gagtttttgc attaattatg tatattggtt cacctctatt
     3721 tgcttcactt agtaaaggag ggcaagaact cgttccaata ttgcgtagtc taactctagc
     3781 tgttttagtt tttccttcaa tgagtgtttt acgtggtttt tttcaaggtt ttaataattt
     3841 aaaaccgtat gccattagtc aagtcgctga gcagattata cgtgtcatat ggatgttatt
     3901 aactgctttc tatattatgc gtctaggatc aggagattat attgctgctg tgacacagtc
     3961 aacattcgcc gcctttgtcg gtatgtttgc tagtatagct gtgctgcttt atttcttatg
     4021 gcgttataat atgctatctg ccttgattag taaaactcca gagcacatta aattagatac
     4081 taaagaaatc ctaattgaga ctgttaagga agcaatccct tttattatta ctggtgcagc
     4141 aattcaaatt ttcaaattga ttgatcagtt ttcatttggg aatacgatgg cgctctttac
     4201 gaattattca agtgaagaat tacgggttat gtttgcttac ttttcaagca accctggtaa
     4261 agttacgatg attctaattg ctgttgctac cgcaattgct ggagttggta ttccattgtt
     4321 aacagagaat tttgttaaga atgataaaaa agccgccgct cgtttggttg ttaataattt
     4381 acaaatgtta ctaatgtttt tactgccagc tgttgcagga tccgttattt tagcaaaacc
     4441 tttgtataca gtcttttatg ggttaccaca gggacaagct ttagggcttt ttgtcatatc
     4501 tcttattcaa acaattattt tatctattta tactgtttta gcaccaatgc tacaagcatt
     4561 gtttgaaaat cgtaaagcta ttatttactt cctatatggt ctggtggcaa aagttatttt
     4621 acaagttccc tccattttcc ttttccatgc ctatggacca cttttctcaa cgacagtagc
     4681 tctttgtatc ccagtaattc ttatgtattt gaaaatacat gaaattacag gatttaaacg
     4741 ccaagctatt cgtcgtacaa gtgctttagt ccttattcta acgctattga tgagctttat
     4801 tatttctatg ataatatggt taatgaacct ggttattgta cccgatagta gactggtaag
     4861 tctagtttac attatcgtta tcggtgcaat aggtttaggg gtatatggct ttatggcact
     4921 ggccacccat ctacttgata agatgattgg tagtcgtgct caagacttac gccgaaagtt
     4981 gcaccttaac taatgaaaaa ataggttaga cagaattgtt taacctagtt ttttgacgac
     5041 caaaattatt tgtgaaattt taaggtagtc acaatatagt gagcttattt ttatgttata
     5101 attaaatgga gaaatatgag gtgaaagatg tcttacgaaa agcttttaga acgattttta
     5161 acatacgtca aaataaatac aagaagtaat cctaatagta cacaaacgcc aacaactcaa
     5221 agtcaagttg actttgcttt aacagtttta aaaccagaaa tggaagcaat tggtttaaaa
     5281 gatgttcatt atttaccttc taatgggtat ttggttggaa ccttacctgc tacaagcgac
     5341 cgcttacgcc ataaaatagg ttttatatcc catatggata cagctgattt caatgctgaa
     5401 aatattactc cacaaattgt tgactataaa ggtggagata ttgaacttgg agactcaggt
     5461 tacattttaa gtccgaaaga ttttccaaat ttaaataatt accatgggca aacattgatt
     5521 acaacagatg gtaaaacctt actgggagca gacgataagt ctggtatagc agaaatcatg
     5581 acagctatgg aatatttggc ttcgcatcca gaaattgagc actgtgaaat tagagttggc
     5641 tttggaccag atgaagaaat tggtataggt gcagataaat ttgatgttaa agattttgat
     5701 gttgattttg cctatacagt ggatggtgga ccactaggag aattacagta tgaaaccttt
     5761 agtgcagctg gtttggagct tacatttgaa ggacgaaacg ttcaccctgg aactgcaaaa
     5821 aatcaaatga ttaatgcttt acagcttgct atagattttc atagtcaatt accagaaaat
     5881 gaacgtcctg aacaaacaga tggctatcaa ggattttatc acttatatga tttaagtgga
     5941 acagttgatc aagctaaaag ttcatatatc attcgagatt ttgaggaagt taatttctta
     6001 aaacgtaagc aattggctca agctatcgct gataatatga atgaagcatt acaatctgaa
     6061 cgtgtaaagg ttaaactata cgatcaatat tacaacatga agaaagttat tgaaaaagac
     6121 atgacaccta tcaacattgc taaagaagta atggaagagt tagacatcaa gccgatcata
     6181 gaaccgattc gtggtggtac agatggctct aaaatttcct ttatgggaat ccctactcct
     6241 aatctttttg caggtggtga aaacatgcat ggacgctttg aattcgttag tctacaaaca
     6301 atggaaaaag cagttgatgt tattttaggc atcgttgcta aggattagta aagaaatatc
     6361 agattggagt ggttaaatga taaaattatt tgggaagata cgttatcact ggcaacctga
     6421 actttcttgg gctattattt attggtcaat agccattgca ccaattttta ttggtttatc
     6481 tctgctatat gaaagaactg agattccgag tcaggtattt gttctatttg ccatctttat
     6541 tgttttagta ggcattggat ttcatcgcta ttttgttatt gaagaagatg gatatcttag
     6601 gattgtatct tttaactttt taagacgatc taagtttcca attgaagata ttgctaaaat
     6661 tgaggtcact aaatcaagtg tcactatcaa atttaataat aaccatgagc gtatttttta
     6721 catgcgaaaa tggcctaaaa aatatttttt agatgcacta gctattgaac ctaaatttaa
     6781 gggagaagtg gagcttctcg ataatttgat taaaatggat tattttgaat gttatcgcta
     6841 cgataaaaaa gctcttacaa aatcgtaaga gctttggtgg gacatgattt aactgctaaa
     6901 acagtatctt gatcagaact tggaatttct ttttctaggt tatctgtatc ggaaaattta
     6961 acgataccat catcttgata atcaaaaata tttgaatatg tttggcaaag cccgcaggca
     7021 atacattttt ctggaattag tgcaactttc atagtattat tgtaatactt attgatacga
     7081 ttaacaaggg ggaagtcatg gcgaaaaaac catgggaaaa aaaagttgtg gaaaacaata
     7141 gtcatagaaa agacaaaatt acaagaacga gtagaggtgt ggtgtcaagt actccttgga
     7201 ttaccgcttt cttgagtgca ttctttgtta ttgtcgttgc tattttattt atagtatttt
     7261 atacttcaaa tagaggagaa gacagagcaa aagaaacttc tggtttttat ggtgcttcaa
     7321 gtcaaaaagt taatagttct aaaacgaaaa aagcatctac aagtaagaaa acaacgtcat
     7381 catcaaaaac taaaactagt gataaatcaa caacatcttc tagtacagta gcaagttcga
     7441 gtgagacatc gacatcatct tcagtaaacg gtggaacaat cgtagttcaa ccaggtgagg
     7501 gagcagcctc catagctgct cgtgcaggta tctcagttgc tcgtttacaa gaacttaatc
     7561 ctggtcatat gacctcaggg tattggtatg ctaatccagg cgatcctgtg gttgtaaatt
     7621 aaggggagga ttatgaactc aattaatatt gctattgatg gaccagcatc aagtgggaaa
     7681 agtactgtcg ctaagattat tgctaaaaat ttgaattaca cttaccttga tacaggtgct
     7741 atgtatcgtt gtgctactta tctggcttta cagcatggat atgaagcaca agatgtttcg
     7801 aaaattttag gactcctagc tgaaagaccg atttcttttg gtaaagcaga agatggcagc
     7861 cagacagtct ttcttggcac agaagaagtt actttagcta tccgtcaaaa cgatgttact
     7921 aacaatgtct cgtgggtttc tgctatccca gagattcgag aagagttagt aaaccagcag
     7981 cgtcgtatag ccaaagatgg tgctattata atggatggtc gtgacatagg aacagttgtt
     8041 ttgccagatg cagaattgaa gattttttta gtggcttctg ttgatgaaag agctgagcgt
     8101 cgctttaaag aaaatcaaga aaaaggcatt gagagtgatt ttgaaacact gaaatcagaa
     8161 attgctgctc gtgattataa agatagccat cgagaagttt caccattaaa ggcagcagaa
     8221 gatgctattg aatttgatac tacaggagta tcaattgaag gtgttgtaac ttttattcaa
     8281 gaaaaagcag aaaaaataat tgacatgaag aattagtcat gatatactag ttgcaatgag
     8341 aaaagcagaa gtgagaactt ctcgccttgc gacttaggtt gtctggccca acatgtcaag
     8401 attccatcta ggaagattat atgtgcgggc ttgatttatc aagtccgctt tcgtttttct
     8461 ctaaaaaaat aaagaggtga agatcatagc taaaaaagat ctattcatta acgatgaaat
     8521 tcgcgttcgt gaagttcgtc tggttggtct cgagggtgaa caattaggta ttaaaccatt
     8581 gtctgaagct caagcaattg cagatgatgc taatgttgat ttggttttaa ttcagccaca
     8641 agctacacca cctgttgcga aaattatgga ctatggtaag ttcaaatttg agtatcaaaa
     8701 gaaacaaaaa gaacaacgta aaaaacaaag cgttgttact gttaaggaag ttcgtcttag
     8761 cccagttatt gataaagggg attttgaaac aaaacttcgt aacggtcgta agttccttga
     8821 aaaaggtaac aaagttaaag tttctatccg attcaaagga cgtatgatta ctcataaaga
     8881 aattggagca aaggtattgg cggaatttgc tgaagcgaca caagatatcg ctattattga
     8941 gcaaagagct aagatggatg gtcgccaaat gtttatgcag cttgcaccaa ttccagacaa
     9001 gaaataattg tcgactttat agtattattt aagaggagaa tttaaaatgc caaaacaaaa
     9061 aacacaccgc gcatcagcta aacgctttaa acgcacaggt tcaggtggat tgaaacgctt
     9121 ccacgcgttt acttcacacc gtttccacgg taaaactaaa aaacaacgtc gtcatcttcg
     9181 taaagctact atggtttcat caggagattt caaacgtatt aaagcaatgc ttactcgcct
     9241 taaataataa ggtctctgta aacatttact acatattata aaggattatt tggaggaaat
     9301 ataaatggct cgtgttaaag gtggagttgt ttcacgtaaa cgtcgtaaac gcgtacttaa
     9361 attagctaaa ggttactatg gtgcaaaaca tatcttgttc cgtactgcaa aagaacaagt
     9421 aatgaattct tactactatg cataccgtga ccgtcgtcag aaaaaacgtg acttccgtaa
     9481 attatggatc actcgtatca atgcggctgc tcgtatgaat ggtttatcat attcacaatt
     9541 gatgcacggt ttgaaattag ctgaaatcga agttaaccgt aaaatgcttg ctgatttagc
     9601 agttaacgat gcagcagctt tcacagctct tgcagatgca gctaaagcta aacttggtaa
     9661 ataagaaata aaaaagttgg gtctgcctaa ctttttttaa ttactagaaa gaaagtacct
     9721 atattataat ttaagtgaaa agtttcgttc tatatatgta gaatgaaact ttttttgttt
     9781 tattccgata cctttattat tgttataatt ttcctttttg atttttattg ttttatagta
     9841 ttattccgta taaaaagcac ctcaatcgtt ggattgaggt gtggattaaa ctttaaatgg
     9901 taaagattat tttttagatg ttttatgtgt cttattagga tcttgcaatt ccaagtaact
     9961 tggagcgtgg aagagatcaa ctgttgattt gttgttatgt ttgctgtata tgcttgttga
    10021 acgatctttc aatttacgtt ctttagcttt gagtgcttta agagaatgtg tgtaattgaa
    10081 tttttcaact tctacagttt tcaaaccatt gttttcatca aatcgtagta aatctccagt
    10141 ttgaatcgaa tcacttgtag ataattgttt attagtagca tctcgaatag tttttatttc
    10201 agctttagtt gtttcatcag gatttgtgat ttcttgtcct gaatcagtgt aatagagatg
    10261 tccactatag ttagtgtatt taggagtaat atactgacca gttgttctca tagcgaccat
    10321 ttgtttatta tctttagata acaagtcttg tcctaactga gtatatttag acgtgtctat
    10381 ccctagaata tgaagtaagg ttggtaaatt atctacctca ccaccgtatg tattactgat
    10441 aaaacctttt gaatagccag gaatgtggat catatatgga acacgttgta acatagcgtt
    10501 gtcgtattca gaccatgttt cagggtcttt acctaataat tcagctaagc taggattgcg
    10561 tgtattagaa ataccgtagt gatccccata cattacaata atagaattat catacacacc
    10621 agcagctttt agatattcaa aaaatgattt aagagctgta tcaaggtagt ttgctgtggc
    10681 gaaataaccg ttaatcgttt catcgttagt ctttgcaagt gggaatccct cttcgtcaga
    10741 ctctcccttc aaacttgtgt aagggtaatg gttacttact gtaataaatt tagtataaaa
    10801 cggttgttgc atgtgctcta gatatttgat agagtcagca aacatatatt tatcattcaa
    10861 accgtattgg aatgaattat ctttggtttg ttttgagaag tagctagagt caaagaagta
    10921 gtcataaccc cactgtttgt aagcattatt tctattccaa aatgttccga cgtttccgtg
    10981 gaagaccgca ctactgtaac ctccattttg agctaagata tgtggagcag caaattgtgt
    11041 attttcacca ccataattaa ccatgaatga accactactt agaccaaaaa gtgagttttc
    11101 catgagtgtc tcagcatcgg atgtcttacc agctttgact tgatggaaga agttagagaa
    11161 ggagatagtc tctttcgaat gataaaggct gttaataaat ggagtaacca catgttcttt
    11221 accatcaaca tttaatttat agtcaatcaa gaattgttga aaactctcca aatgaatcat
    11281 gataacgttt cgcccttttc cgataccata atattctgga ttgggtttgg cataatgctc
    11341 tgctacatat tttttggcag tcgctaattc ttgtgcagtg gcaccatttc tttctttctg
    11401 tgcttgataa gtttgattac cactataaat cgaaaatgat ggcaagccaa gagctttaac
    11461 aatataggtg tttgaaaaac cacgacttag tagttctgga cgatcaattt cagctaagaa
    11521 taagttaatt gaaaagagta aaccagacaa agctgtgata gaaaaactag cacgtttatt
    11581 aaatggacga ttatctaagt ggattttctt ggttgcaaat aagaaaataa ggataatgaa
    11641 atcgaaaacg tagacaagat cccaaattct caatagatta agagcagagt ctccaaggcc
    11701 agccgaagtt ttactactag ccaagacagc gctaactgta atgaaatcgg aaaactctct
    11761 atagtagatt gcgtttgcaa ttagaaggat atttacgata gcataggtga taaaagcggt
    11821 aatataaaaa gctttggttc gcttaacata taaggccaaa cctatgatta agagtcctaa
    11881 ggggatgggg tttataatgg tcaaaaacaa ctgataggaa ttttctaaac ctaaattaaa
    11941 atcagtatgg taagcccaaa ttgtctttaa ccaatatata actactaaca ataaaataaa
    12001 gccgagtcgt gtattgatcg cacggctagc aaaatcttta atttttttca cagtaggaac
    12061 ttcctttaat ttttttccta aagataaaat ctcagattaa attataacat aaaattttgt
    12121 aacatacggt aaagaaaggt cgaattcagt atgaaaaaag ataaaaattt gttataatac
    12181 tgtttatgaa taaacttaaa gtgaattctg tcgttgaaag aaaaatcaaa tcaggtgctc
    12241 agttactgga aaaaaaagat tttgatacca gtttagttaa ccagttggtt caactttttt
    12301 cacagtcaaa tcaattctta gggatggcct atctttcacc acaaaataaa gggattggtt
    12361 ggttactatc aagacaaatt tttgatttta accatgatta ctttgtatcg ctattcgaaa
    12421 aatctagaga gaagcgtcaa aaatttgaaa aatctagcca aacaacagcc taccgcttgt
    12481 ttaatcagga tggagataac tttggtggcc taactataga tttttatagt gactatgctc
    12541 ttttttcgtg gtacaatgaa tttgtttata ctaatcgaca aatgattgtt gcagccttta
    12601 agcaggtcta tcctaatatt aaaggggcat atgaaaaaat tcgtttcaaa ggtttagact
    12661 ttgaaagtgc tcatttgtac ggtcaagagg ctcctgaatc atttttgatt ttagaaaata
    12721 atatcaaata tagtgtcttt ttgaatgatg ggttaatgac aggtattttc cttgaccaac
    12781 atgatgtcag aaaagcctta gcaactaatc tatcagaagg taaaaaagtt ctaaatatgt
    12841 tttcatacac tgcggctttt tctgtagctg cagctgttgg aggagcatta gagacaactt
    12901 ctgttgattt agcaaaacgc tcgcgtgaac tttcaaaagc acactttgat gctaatcaga
    12961 ttgtcacaga taaccatcga tttatcgtta tggatgtttt tgaatattat aagtatgcca
    13021 aaagaaaaca tctatcatat gatgtgattg ttattgatcc accaagtttt gctcgaaata
    13081 aaaaacaaac tttttcggtt actaaagatt actataaatt aattgaacaa gctttagata
    13141 ttttagcccc tggtggaact atcattgcat caactaacgc agctaatcta accgtatcac
    13201 aatttaagaa acaattggaa aaggggtttg gtaaagcttc acataattac attagcttac
    13261 agcagttgcc tgaagatttc actgtaaatg ataaggacca acaaagtaat tatttaaaag
    13321 tatttacaat aaaggtaaaa taatgaaaat agtagtacca gtaatgcctc gcagtcttga
    13381 agaggctcaa gaaatagatt tatcaaaatt tgatagtgtt gatattattg aatggcgagc
    13441 tgatgcctta ccaaaggatg acattattaa tgtagctcca gctatttttg agaaattcgc
    13501 aggtcatgaa attattttta ctcttcgtac aacgcgtgaa ggtggtaata ttgtcttatc
    13561 tgatgctgag tatgttgagt taatccagaa aattaattct atctacaatc cagattatat
    13621 tgattttgag tatttttcac ataaagaagt ttttcaagaa atgctagaat ttccaaattt
    13681 agtcctgtct tatcacaatt ttcaagagac accggagaat attatggaga tattttcaga
    13741 attaacagcc ctagcaccac gagttgtgaa aatcgcagta atgccaaaga atgaacaaga
    13801 tgtcttagac gttatgaatt acactcgcgg tttcaagact attaatcctg atcaagttta
    13861 tgcgacggta tctatgagta aaattggacg tatttctcgt tttgctggtg atgtaactgg
    13921 atctagttgg acatttgcat atttagattc atctatcgca cccggacaaa ttactatttc
    13981 agagatgaag cgtgtcaaag cattgcttga cgctgactga tacgagataa caaaatgtta
    14041 aatatactga tgagatccct agcaattgct tagggttttt gtcttattaa aacaaacatg
    14101 agaggacatt agaatgcagg ttgaagttga cttaccgaat cacccttatc atattaaaat
    14161 tgaagaagga tgtttttctg aggctggtga ctgggtatca catttatggc agaaacaaat
    14221 gataactatt attacagata gtaatgtaga aatattatac ggggagtcgc ttgtcaacca
    14281 gttaaaaaaa caaggtttta cagtacatgt ttttagtttt gctgcgggtg aagctagtaa
    14341 aactttggaa gttgctaatc gtatttatgg ttttttagca aagcatcata tgaccagaag
    14401 tgatgggatc attgcacttg gtggaggcgt cgtcggagat ttagcagcgt ttgttgcttc
    14461 tacttatatg cggggaatcc attttcttca aattccaact agtttaacag cacaggttga
    14521 ttcttccatt ggcggaaaaa caggagtcaa cacttcattc gctaaaaata tggtaggtac
    14581 ttttgcacaa cctgatggtg tactgataga tcctgttact ctaaaaacat tgggcaatcg
    14641 tgaattagtt gaaggtatgg gagaagtcat caaatatggc ttgattgatg atataaaact
    14701 ctggcatatc ttagaagaga tggatgggtc tattgatagt atccttgata atgcattagc
    14761 tattatttat cactcttgtc aagttaaacg aaagcatgtt ttagctgatc aatatgataa
    14821 agggttaagg atgcatttaa attttggcca tactatcggt catgctatcg aagtacatgc
    14881 tggctatgga gaaataatgc atggagaagc tgttgcaatt ggaatgatcc agctttctcg
    14941 tgtggctgag cgtaaaaatc ttatgccaag aggtatcagt caagatattt ataatatgtg
    15001 tttaaaattt ggccttccgg tccactatgc agaatgggat aaggacgtct tatttaacat
    15061 attgagtcat gataaaaaag caagtggaca atttattaaa attgttatac tgcctcaatt
    15121 aggtagtgca acagtccacc aaataccttt agaagaaatg agagattatt tggagaaata
    15181 gatgagatat ttaactgctg gtgagtcaca tggacctagt ttaacagcta ttatcgaagg
    15241 gattcctgcg ggactgaaat tatctgctaa agatattaat gaagatttaa aacgccgcca
    15301 aggtggttat ggtcgtggga atcgaatgaa aattgaaact gatcaagtga tcattagttc
    15361 tggtgtgcgc catggaaaaa ccttgggttc acctattaca ctgacagtaa ctaataagga
    15421 tcatagcaaa tggctggaca tcatgtcagt cgaagacatt gaagagcgtc taaagcaaaa
    15481 acgtcgtatt aagcatccta gacctgggca cgcagattta gtaggtggaa tcaaataccg
    15541 atttgatgat ttgagaaatg ctttagagcg ttcttcggct cgtgagacca caatgcgagt
    15601 agctattggt gcgattgcta aacgtatctt gaaagaaatt ggtatcgaaa ttgctaacca
    15661 tattgtagtt ttcggtggca aggagattac tgtccctgat aagctaacag ttcaacaaat
    15721 caaagtgtta tctagtcaat cgcaagtagc tatcgtcaat ccatcatttg aacaagaaat
    15781 taaggattat attgacagtg tgaaaaaggc tggagatact atcggaggag tgattgaaac
    15841 tatcgttgga ggagttcccg ttggtttagg ctcttatgta cactgggata gaaagttaga
    15901 tgctaagata gcgcaagcag ttgtatctat caatgctttt aaaggtgttg aatttggtct
    15961 aggttttaag tcagggtttt taaaaggaag tcaagtaatg gactccatat catggacaaa
    16021 ggaccaaggt tacatacgtc agtcaaataa tttaggcggt tttgaaggcg gtatgactaa
    16081 tggagagcct attgtcgtta ggggagtaat gaagccaatt cctacgcttt acaagccact
    16141 aatgtcagtt gatattgata ctcacgagcc ttatagagct acagtggaac gttctgatcc
    16201 cacggcactt ccggctgcag gtgtcgttat ggaagctgta gtagccaccg tcctggtgac
    16261 agaagtgcta gaaaaatttt cttcagacaa catgtatgaa ctaaaagaag ctgtaaaact
    16321 atatcgcaat tatgttaatc atttttaagt gaaaactagt agcattcaat tgcaaaaaaa
    16381 ggtataaaag tctacaatag tggtaagttc aagtagaaaa aggagtaact tatggctaat
    16441 gtatatgatt tagcaaatga attagaacgt gctgttcgtg ctttaccaga ataccaagca
    16501 gttttaactg caaaagcagc tattgaaaat gatgcggatg cacaagtgct ttggcaagac
    16561 tttttggcta cccaatcaaa agttcaagaa atgatgcaat ctggccaaat gccaagtcaa
    16621 gaagaacaag atgaaatgtc taaacttggg gaaaaaattg aatccaatga ccttttaaaa
    16681 gtttattttg accaacaaca acggttgtct gtctatatgt ctgatatcga aaaaattgtc
    16741 tttgcaccca tgcaggactt gatgtaatag tataaaagag agagctcctt gagttttctc
    16801 ttttatgcta tttacattca gagcatccta attgctgcgc ttttacatta ttgcattatc
    16861 c
//
LOCUS       DATROY010000032        16708 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.032, whole genome shotgun sequence.
ACCESSION   DATROY010000032 DATROY010000000
VERSION     DATROY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16708
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(202..861)
                     /locus_tag="VBD11_000858"
     CDS             complement(202..861)
                     /locus_tag="VBD11_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6953823.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
     gene            complement(880..1590)
                     /locus_tag="VBD11_000859"
     CDS             complement(880..1590)
                     /locus_tag="VBD11_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953824.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            complement(1590..2354)
                     /locus_tag="VBD11_000860"
     CDS             complement(1590..2354)
                     /locus_tag="VBD11_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953825.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            complement(2355..3308)
                     /locus_tag="VBD11_000861"
     CDS             complement(2355..3308)
                     /locus_tag="VBD11_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6953826.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            complement(3311..4180)
                     /locus_tag="VBD11_000862"
     CDS             complement(3311..4180)
                     /locus_tag="VBD11_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6953827.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            complement(4286..5452)
                     /locus_tag="VBD11_000863"
     CDS             complement(4286..5452)
                     /locus_tag="VBD11_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6953828.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            complement(5607..5852)
                     /locus_tag="VBD11_000864"
     CDS             complement(5607..5852)
                     /locus_tag="VBD11_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO6953829.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(5952..6542)
                     /locus_tag="VBD11_000865"
     CDS             complement(5952..6542)
                     /locus_tag="VBD11_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6953830.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(6667..7296)
                     /gene="upp"
                     /locus_tag="VBD11_000866"
     CDS             complement(6667..7296)
                     /gene="upp"
                     /locus_tag="VBD11_000866"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6953831.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(7406..8575)
                     /locus_tag="VBD11_000867"
     CDS             complement(7406..8575)
                     /locus_tag="VBD11_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6953832.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(8711..9259)
                     /locus_tag="VBD11_000868"
     CDS             complement(8711..9259)
                     /locus_tag="VBD11_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6953833.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(9432..10784)
                     /locus_tag="VBD11_000869"
     CDS             complement(9432..10784)
                     /locus_tag="VBD11_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6953834.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            10990..12339
                     /gene="trkA"
                     /locus_tag="VBD11_000870"
     CDS             10990..12339
                     /gene="trkA"
                     /locus_tag="VBD11_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6953835.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            12344..13783
                     /locus_tag="VBD11_000871"
     CDS             12344..13783
                     /locus_tag="VBD11_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6953836.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(13807..14058)
                     /gene="yidD"
                     /locus_tag="VBD11_000872"
     CDS             complement(13807..14058)
                     /gene="yidD"
                     /locus_tag="VBD11_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6953837.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(14058..14780)
                     /locus_tag="VBD11_000873"
     CDS             complement(14058..14780)
                     /locus_tag="VBD11_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6953838.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(14770..15354)
                     /locus_tag="VBD11_000874"
     CDS             complement(14770..15354)
                     /locus_tag="VBD11_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6953839.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(15351..16058)
                     /locus_tag="VBD11_000875"
     CDS             complement(15351..16058)
                     /locus_tag="VBD11_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6953840.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(16058..>16708)
                     /gene="xerD"
                     /locus_tag="VBD11_000876"
     CDS             complement(16058..>16708)
                     /gene="xerD"
                     /locus_tag="VBD11_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6953841.1"
                     /translation="KIIGGHVNSEKLALYQQSLSEFKLTARKRKLSAVNQFLFFLYNR
                     GTLKEFYRLQETEKITLAQTKSQIMDLSNFYQDTDYPSGRLIALLILSLGLTPAEIAN
                     LKKADFDTTFNILSIEKSQMKRILKLPEDLLPFLLESLEEDGDLVFEHNGKPYSRQWF
                     FNQLTDFLNEKNEQQLTAQLLREQFILKQKENGKTMTELSRLLGLKTPITLERYYR"
BASE COUNT         5392 a         3331 c         2546 g         5439 t
ORIGIN      
        1 tctgaaacgc tttcagatga ggatgttttc ttgtaccctt tttttgcgat ttttaactct
       61 gaaaacctat gaaatcaagt attataaaaa ccaacggaac ttagtctgag acggattttc
      121 gttggttttt attggttttt atcagactaa attccatttc tactagaggt ggaacttact
      181 ttgagaattt agctgatatt tttaagaaag tggttttttc atggtctttt ctaaagtttc
      241 cacaataaca tcatcaaata ctagttcttg ttttaaaaca tccttgtcag cgtatccttc
      301 tagttgtatc tcgaccgctt tcttacctgc ttttcgttca ataatgacta aacgtttaac
      361 acttaaattt gcactagaaa gacgatttag gacttttgat agtaccccta tcccctcatc
      421 agctaatata accaagcgat aaccttcttg accatacccc gcaatttcaa ggaaggcttt
      481 aaaaacatcc ctatccgtga caatcccata aagttgtcca ttatcaacta ctggtaaaac
      541 accgatcttt cgagacatca tcaaataaat agcgtcctca aggctagcat actgtgaaac
      601 agtcacaata tctttaatca taatatctcg aatttttgtc ttattaagaa gatagttcat
      661 ctcatatata gaaagactgg ttgctttaga aggttgtgct tctgccattg tcccttctgt
      721 cactaacccc actagctgat cattttcaac aactggcaaa cgccttaagt ggtgttctct
      781 caataaatca gcagcttctg caactgtagt atctggagag acataaacta atttttttgt
      841 cataaaatct ttcactggca tacctgactc ctctaaatat tatccaccta agtaagcttt
      901 acgaacttga tctgaaacta gtaattcctt acctgtccct gataaaacaa ccttcccagt
      961 ctctagaaca tatgctttat cagcaatcgt cagagcttta tttgcatttt gttcaactaa
     1021 aagaactgta gtcccttgtt ttttgatgtc ttcaatgata ttaaaaattt cttgaataaa
     1081 aattggagct aggcccattg actgttcatc taaaagtaat aatttaggac gactcatcaa
     1141 tgcacgcccc atagcgagca tttgctgctc accacctgat aaggttgctg catcttggtt
     1201 tttacgttct tctaagcgag gaaaacgatc aaacactttt tttaacattt tttgattttg
     1261 ttcacgatcc ttttgaagaa aggctcccat ttctaaattt tccataacag acaaacttga
     1321 gaaaacgtgg cgcccttctg gaacctgagc taaaccattg ccaacaattt ttctagctgc
     1381 taacttatgt ataggtttcc ccataaaaga aatagaaccc tgacttggtc ttactaaacc
     1441 agaaattgtc ctcaaaattg atgtctttcc tgcaccattg gctccaatta aggttacaac
     1501 ctcaccttgg ttaacttcaa acgagacatc attcaccgct tgaataacac catagtgaat
     1561 agatagattt tctactttca acatggtcat tatagctccc cccccaagta tgcttctata
     1621 acacgtttat tatttttaat ttcttcaggt gtcccgtgag caatcagacg accatattcc
     1681 aatacataaa ttctctctgt tacttgcata accaagttca tatcatgttc aatcagcata
     1741 atagtaatat caaaatcact ctttatttga ctaattaact gcgtcaattc tgcagtttct
     1801 tgaggattca ttcctgccgc gggctcatcc aaaaacaata tttttggttc tgttgctaaa
     1861 gcacgaacaa tttctaaacg ccgttgtttt ccataaggga ggttcttagc aagagcatct
     1921 tgatatgctt tcaaaccaaa aatctctaat agctccagtg cctttttcct taaggctttt
     1981 tcactatgat agtatttggg taatcgtaaa aaactagcaa taggatgtga taagtgatga
     2041 ttactgagtc ctactagtac attgtctaaa acagtcatat ttttaaaaag tcggatattt
     2101 tgaaaagttc tacctaaacc taaagaagct attttagctg gtttcctccc atttaatatc
     2161 ttaccatcaa gtgatattgt ccctttacta ggaaggtata ctcctgttaa gagattaaaa
     2221 agcgttgtct tacccgcacc gttggggccg attaagccaa ttaattcacc tttatgtaat
     2281 ttcatcgaaa catcacctac agcagttagg ccaccgaaat gtttacttaa attttttact
     2341 tcaagaagtg ccatctaatt gcctccctta ctcgtcttac gaaatagatg agatagagtt
     2401 aactctttcg ttcctaataa accgctaggt ctaaaaatca taattaaaat cagggctaaa
     2461 gcatagataa tcattcgaac agaagcgtag tcttgtagta acatattcaa taatcctaat
     2521 aaaattgctg ctaaaactgt ccctgtcatt gaacctaaac cacctaaaac aacaataatt
     2581 aaaacattga cagacatcat aaatgaaaag tcttttggca taacagttcc aacgtagcca
     2641 gcctgtaaac taccagcgat agaagctaaa atagctccaa aaacaaaaac aattactttc
     2701 acttttgttg tatccacacc catagactct gctgcaattt catcttctcg tatagcaatt
     2761 gtattacgcc ctaagggact acgcaaaaaa ttcattgcaa ggataaggct cacaactgcc
     2821 accccataaa ctaaagacca ggttgtaaaa ggcggaatcc ccatgatacc cgcagcacca
     2881 ttcgtaatat cacctccatt aaccaacaaa atacggataa tctcagcaac acctaaagta
     2941 gctatagcta aataatcacc ttttagtctc aaagtaggaa ttcctacacc taaggcaata
     3001 cctcctgcaa caagagttcc aacaagtata gctatcaaaa aacctacata agttggcatt
     3061 ttaacgccga taatagcaac tgcataagcg ccaatagcca tgaaacctgc atgtcctagt
     3121 gaaaactgtc ccgaacaacc tacaattagg tttaaaccaa ctgctaaaat aacattaatc
     3181 ccaattgttt caataatttg aatatgatag aggttaaata tacccataga tattagcacg
     3241 ctaatcaaga gataacctaa cactactata gctaaccaag ataaaattga tttcatattt
     3301 tctttcatgc ctagaccttc tccttaatat tttttccaag aattcctgct ggacggatca
     3361 agaaaatgag aattaaaata gcataaacga taccatctcg aaaatctgac acaccaagtg
     3421 cagtcgctaa agtttctaaa atgccaataa caaaaccacc gatagccgct cccggaatga
     3481 taccaatacc acctaagaca gctgcaacaa atgctttcaa ccctggagtt acccccataa
     3541 gaggttgaac agaattataa taaagcccaa tcagtactcc acctgctcca gctaaagcag
     3601 accccagcgc aaatgtaaaa ctaatggtac ggttaacatt aattcccatc aattgtgcgg
     3661 catcgctatc cactgataaa gctctcatag ctttccccat tttagttttt tgaacgatga
     3721 actgtaaagt aagcattagt aatagcgcaa tacctaaaat gatgagctga acattggtta
     3781 tagttatcgg tcctaaatta tatttaacag tatgaatggc ttgcggaaat gccctagtat
     3841 ccgctccaac aaggtatacc atcccgtact ccaaaagaaa agaaacacca atagctgtaa
     3901 ttaacgcggc aattctagtt gattttctca gtggacgata agctaaatat tcaattacaa
     3961 cacctaggaa agcagacccc agcattgcaa ttaataaagc taggaaaaaa ttaagatgaa
     4021 gatgattaat caaataatag cccatgaaag cgcccatcat atagatgtcc ccatgagcaa
     4081 aattaatcag cttaataatg ccatatacca tagtatatcc taaggcaagt aaagcatata
     4141 tggatcctaa gataagtcca ttaacgagct gttgaagcat attataacca tactttctat
     4201 tatatattat tgtcgaatta ttctgaaaaa ttacttttta aaaaagggga gctgaaaatt
     4261 aaactcccat ttttctagag aatacttatt ttgctgaaat aatgttaacg ctacttgttt
     4321 ttccatcctc taatttgaca acgtaagctg atttaacaac attgtgattt ttatcaatag
     4381 acattttccc tgtcacacct ttaaaatctt tcagcttagc taaagctttt ttaatatcaa
     4441 ttgaagtttt agctccttta gcagatttag ctgccatata cacagcgtcg tatgataaag
     4501 ccgagaacat ggatggttct tcaccatatg ccttaacgta atgatcatgg aaagctttag
     4561 ctttggttga tccttgtgta gtgaaacctg tcaaataata aacgtttgaa gctcctacag
     4621 gtgttgccga ttgcacaaat ttcggactat caaaaccatc aggcccaaga accggtttag
     4681 agatacctaa atcacgcgct tgcttaacta ttaatcctgt ctcggtatag taacctggca
     4741 tcacaatagc atcatattct ttccctttca acttagtcaa tgacgcttgg aaatcagtat
     4801 caccagcgga gaatgtcata ctatcaacaa tttttccact ataactttcc ttaaatgatt
     4861 ttgctacccc ctttgagtaa tctgatgaat tatcataaaa tagaacaact tttttagctt
     4921 taaggttgtc tgtagcatac tgagacaata gctttccttg ataattatcg acaaaagtag
     4981 ctcggaagaa atactgattt aaggtttttc catcagaacc atagactaaa ttgtcttgtg
     5041 tcgcagcagg tgcaatcatt ggaactgctg ctgcatttac ttttggagtt gaagatgcag
     5101 ctgcacccga tgttgctggt ccgataatga cattagctcc tttggtaacc aagtttgtgg
     5161 caaccgttgc tgcctctgca ttttctgatt tattatcctg tgataggact tttaacttct
     5221 tgccgccaac ccctccagca gcattaattt cttttactgc taagtcagct ccattcttca
     5281 ttgaatttcc atatgaagaa acgtttcctg ataattccca gttataccct accttaatat
     5341 ctttccctat ttttgttcct gtcgaagatg ctcctcctcc gacatttcca catgctgcta
     5401 aaagaactgt actagctaaa acaagcgctc ctaaactaag tctcttttcc atatttttct
     5461 ccttttttga aaatggtaac taaaataata cagaattatc agaatattgt caatagtttt
     5521 taaattgttt ttttgaaaat tatagaaact aaataaaaaa gaaggacatc tgaactcctt
     5581 ctttctaata actactagta aatatcttaa cgatataagt ttccaacaaa atcctgatca
     5641 atatcatcaa ttgctgataa ccgaacatcc ttaacaaatt ttaaatgttt tatctcttta
     5701 ctaacgctaa caatatcctc tttattaaca taaatgacta gatatcttaa tctacgagaa
     5761 tgataatgaa catctccgta tttatataat ttacgagcgt cacgattata gtaaagataa
     5821 acgctcattc ctaaacgctc ttgtttattc aataattttt tcctttctgc gtcatatctg
     5881 taattactaa gattataaca taattccaac aaataaaaaa agagttggta aactcaactc
     5941 ttttaaatct attattgtaa attattattt tccataatag catcaataaa gccataatca
     6001 agtgtttctt gagcactcat ccaacgatca cgctctgcat catcatggac tttttcaata
     6061 gattgaccag aattatcagc taagattttt tctaaagtat gacgcgtttt taaaagatgc
     6121 tcagcagcga tagccatatc agattgctgt gtacctccgc ctgttccgcc cattggttgg
     6181 tggatcatat attctgcatt cggtaacata aaacgttttc ctttagcacc acttgaagca
     6241 ataatggttc ccatcgaagc agccatcccc ataacaatcg tctgtacgtc cgatttaatg
     6301 aagttcatgg tgtccacaat agcaagtcca gccgatactg aaccacctgg tgtattgaca
     6361 taaaggtaaa tatcctttgt attatcttgt gcatcgagaa acaataactg tgcaatgata
     6421 ctattggcca tattatcctc aacttggcct gtcaacataa taatacgatc ttttaaaaga
     6481 cgtgagtaaa tatcataaga acgttcacca cgacttgttt gttcaataac tactggaatc
     6541 atatctttct ccatttcttt ttatcactgt cactattata atcatttggt caacaaaggt
     6601 caaataataa cttttttaag aaaaggcttt ctgagttctt cagaaaagcc ttattcatct
     6661 caaatcttat tttgtaccaa ataagcgatc cccagcatct ccgagtccag gaacaatgta
     6721 gccatgttca ttgagttttt catccaaagc agcggtataa atatctacat ctggatgtgc
     6781 ttcttgaagt gctgccactc cttctggagc tgctactaaa caaacgaatt tgatactagc
     6841 tgcgccacgt ttttttagag aatctactgc taaaatagca gacccacctg tagcaagcat
     6901 tggatcaact acaaaaatct gacgttgatc aatatcttca ggtaacttaa ctaaatattc
     6961 gactggttgg aaagtctctt catcacgata cataccgata tgaccaactt tagcagctgg
     7021 cacaaggctt aagaaaccat caaccatacc aatacctgca cgtaaaattg gaacaattgc
     7081 taactttttc ccagctaatt gtttttgtac agttgtcgct actggtgttt ggatttcaac
     7141 atcttctaaa ggcaaatcac gagacacttc atagcccatt aacatcgcaa tctcatcaac
     7201 taactcacga aaatctttag tagatgttgt agtgcgacgt aagattgaca gtttatgttg
     7261 aataagtgga tgtgaaatga cttgaaattt tcccattata atgaccgctt tctatttaat
     7321 tttgagcatt aaaaacattc taatgcttgt ttacattcaa agtattataa cataaaaacc
     7381 ctttccatgc gacttttttt atagtctagt cattttttct aaagtttgtc actaatcgca
     7441 aaactgcctc ctcaataaca gacctaggag ctgcgacatt gattctagca tgttttttcc
     7501 cttcttttcc aaaagttaaa ccatcattta atattaattt ggcatcatat cgaagttttt
     7561 cttgaatttc taaatgcgtt aaattataag cagaaaaatc taaccaaaca agataagttc
     7621 cctctggttt catcactttg atatgagttt tttgcgttaa ttgctcataa aggtattcta
     7681 ttgatccttc taactccatt tttagggcct ttaaccattg tttccctttt gtaaaggcaa
     7741 cttctgtagc taataacccc aatgaagaaa tttcttgttg attgttagct atttgcctct
     7801 tcttaaaatc cgagcgtaac ttttcatttt caattattgc aaaactattt ttagtccctg
     7861 caatattaaa cgttttagtt gcactagata aaataacact caattcttta aaactagaat
     7921 caacagtatt aaagctatgg tgtacattat caaataatac caaatcttga tgtatttcgt
     7981 cagaaacgag gataacatta tatcgtttac agatatctcc tattttttga atttctcctt
     8041 ttgtccagac acgcccaccg ggattgtgtg gactacaaaa aatataaagc tttacattat
     8101 tttcaatgat atctttttct aattgtttaa agtcaatctc aaaatactga ttattattaa
     8161 gtaaactatt tgaaactaaa tgacgattat tgtattttat agttctagca aagggcggat
     8221 agactggggt attaattaga acagcatctc cctcctttgt gaaggcttgt atagcaactg
     8281 aaatcgctgg tacaacccca tcaattaata ataatgattt tttatcaaat tgataaccat
     8341 gttcttgcac ttcccaatta gagatagctt ggtataaact gtctttagga ctatcatagc
     8401 caaaaaccat ttggtgtgaa aaatcttcaa tagcctccga catttctgga aagataggaa
     8461 aatccatatc cgcaatccaa aggggaagga tttcttgatc tttttgaacc gccttccatt
     8521 ttatggcatt acttgaaaaa cgttctggta aacttgtgaa atcatatgta atcataatta
     8581 ctcctgacta ttctacagct aaaaatatcc cttgtcgcct tggtgactat tttaccattt
     8641 gtttttgata ctatatacta attaacattc taaagataaa agtgaccatt ttaaacgatc
     8701 actttcttta ttattttaat ttatcatttt cataagtatg gcttaattct aaattaggaa
     8761 agctttgctg cctaagggct tcataaacaa tcatacaaac agtgtttgaa acattcaagc
     8821 tacgcacatg ctcatcgttc atgggaattc gtaaggcctt ttcggcatgc tcgcgcataa
     8881 aagtttctgg caatccttta tcttcacgac caaacataaa ataatggtct tgatcatcat
     8941 tgtaattttc atctgagtaa accttgtcag caaatttgct aattaggtga actttccctc
     9001 gacatgacaa cataaattct tccaaaccat catagaaact aacatctagc ttatcccagt
     9061 aatccagtcc cgctcgtttc atttttttat catcaatagg aaatcccatc ggtcgtatga
     9121 tgtgcaaagg agcatttgtt gccgcacaag ttctagcaat attaccagta tttgctggaa
     9181 tctgtggttc aaataaaaca atatgatttc gtccgctatc ggaacgatga tttttctgcg
     9241 ttagtgtttc aatattcatc tcatcatcac tttctagaac tcattctgac tcgcaaaaaa
     9301 accacactgc tcggagtcaa tctcagcaaa caatgtggtt tatggtggtt tgttaactta
     9361 aatcataaca ggtttgattt aaactaacaa atcgttgaca ctattatagc acttcagtct
     9421 acaaagtcaa cctattcatg agtttttggg ttattttttt gatagatata atgacaaatc
     9481 aaacagaata ataagcaaaa acattggaaa attaaaaccg taaagaaatt acttaacaaa
     9541 ctcccgataa ggaggatcaa accgcctaaa attgctagaa tcggtgaaac taagccggta
     9601 aatttatttt taatatcttt tttgagataa aggctgagta ctaaaacata aagaatgatt
     9661 aaactggtat tattaaacac cacagcaatt tcactaatat cactacccgg caataagtta
     9721 aattttgtaa ccataaaatg aacaaaaagc caaaaaatag ctacagccgt caccgtcaaa
     9781 ctagctggca atgacatttg atacttcaaa tttatgttag ccatcctctc acttttgatc
     9841 catccgcgtt ccgcaaaagc ttgaggtaga cgcatcgtcc caagtaaaag tccattggca
     9901 acacctaata cagatagaat aacaatgaaa gataataaac gcccaacact aggaccgaaa
     9961 ataatattag ctgcataatt aatagcatca ttaccggttg tcataataaa gctagcacct
    10021 aaaatttgtg tcaaaccata gaaaaatgcc aaatatgata ataagataag cgctggacca
    10081 atgacaaagg caagtggtaa attcttcttg ggattcttga cttctggtgc aatattaaca
    10141 aaaattgtcc aaccatcata tgcaaaatac aatggtacca gacctgacac ccaactccat
    10201 ccaacatttg acggttgaat agctgttaaa tgttgtggaa gttgtggagc tttatcagac
    10261 caaaaagcac caattaaagc aattagaact aaaggaatca tttttacaaa ggtcgttaaa
    10321 gattggaatc cccctgcaat acgcttagca aagatattca tgagactaag cgctaaaata
    10381 taaacagcag ctaaaataat ctgtgattcc aaacttgatg attcacctaa agtataaaat
    10441 gcagctaccc atgaaacgat tgctgttaaa gttggaagat acaaaaaaga cgcaaacaag
    10501 cctaaagttg ctgctaaagc tgggctaaca tatttttcgt agtaagaaaa aataccacca
    10561 ctttcactcg ttctaatagc tagctcagaa attgacaaac ttccaaaaac aatcgaaaaa
    10621 gaacctagta ctaaaattac catgcctaaa aagacatctc caccagtaaa tttaaggatg
    10681 tcatcaactt taaaataaat gcctgaacca atcactacac ctacaatcat agcaattgta
    10741 gtcattaatc cataggtctg tttaatagaa ttatgattct ccataatatc tccatttcta
    10801 ctgtacgctt aaaggttaat tataacctaa tttagggctt gtcacaagtt tatctgtgtt
    10861 atttttcacc agattctgat aagagaatca tattactctt atcagaaaga tgaaaacgtt
    10921 ttttaaccat aattctcgtg catttttact aaaaaaaagg tatcatagag tttattattg
    10981 gaggtaaaaa tgagaataat tgttgtcggc ggaggtaaag tcggaaccgc actttgtcgc
    11041 tcacttgttg ctgaaaagca cgatgttgtt ttaattgaga aaaaagaaaa cgtccttaaa
    11101 cgtgtcacta aacagcatga tattatggga attgttggga atggagcaaa ctataaaatt
    11161 ttagaacaag ccgaagtcaa aaattgtgat atttttatcg ctatcactga tcgagatgaa
    11221 gttaatatga tttcagcagt tcttgccaaa aaaatggggg caaaagagac tgttgttcgc
    11281 atgcgtaatc ctgagtattc taatccttac ttcaaagata aaaactttct tggcttctct
    11341 tcagttgtca accctgaatt gttagcagca caatatatcg ctaataccat agaatttcca
    11401 aatgcaactt cagttgaaca cttcgccaac gggcgtgtga tgttaatgga atttaaaatt
    11461 ttagaaggaa ataaactctg tcatacttca atgagtcaaa ttcgtaaaaa atttggtaat
    11521 attgttattt gcgcaattga acgcgatggc aaattgatta ttcctgatgg cgatgcaact
    11581 atccaagtca aagataaaat ctttgtaact ggaaatcgta ttgaaatgat acttttccat
    11641 aattacgtta aaaataaagt tgtcaaaaac ttgatggtta tcggtgcagg acgcatcgcc
    11701 tactacctac tcaatatcct taaaaatacc aatacacacg tcaaactagt tgaactcaac
    11761 caagagcaag ctgaatattt tagtcaagaa tttcccaatg ttcctgtcgt tcatggggat
    11821 ggtactgcta aaaatatttt gttagaagaa agcgttacaa attttgacgc agttgctact
    11881 ttgactggtg ttgatgaaga aaatatcatt acctcaatgt tcttagaaag tatcggtatc
    11941 cctaaaaaca ttaccaaagt taatcgaact agcctactag aaatcattga cgataaacaa
    12001 ctatcaagca ttatcacacc caagcgtatt gccgtagacc atgtgatgca ctttgtacgc
    12061 gggcgtgtaa atgctcagga ttccaaccta gaagctatgc atcatattgc taacgatcgc
    12121 attgaaacct tacaatttga aataaaagaa actagcaaat tagcaaatcg tagccttgca
    12181 tctttaaaat taaagcaaaa tattttaatt gcagcgatta ttcgcaataa taaaacgatt
    12241 ttcccaactg gtgaggatgt tttaacagtg ggtgatcgta tcgttgttat caccttatta
    12301 aaaaatatta ctagaactag tgatatgtta gcgaggtagg aatatgaata aaagtatgat
    12361 tcgtttcctt ctctcaaaac ttttgctaat tgaagctgct cttctagcga ttcctctaac
    12421 tgttggactt atttaccgtg aaccgcaaag tgtcatgatg agtatagtca ttaccatgat
    12481 tattttaatc atccttggat tactgggttc tctctttaaa cctaaaaatt atcatatcta
    12541 cactaaagaa ggcatgttaa ttgttgcact ctgctggata ctttggtcct tctttggcgc
    12601 ccttccattt gttatttcag ggcaaatccc taatattatc gatgctttct ttgaagtatc
    12661 ttctggtttt acgacaacag gagcaactat tcttgatgac gtcagtgtct tatcacccgc
    12721 gctcttattt tggcgtagct ttacccatct tatcggtgga atgggggttt tagtatttgc
    12781 ccttgccatc atggaaaatt ctaaaaatag ccatttagag gttatgagag ctgaagttcc
    12841 tggacctgta tttggtaaag ttgtctcgaa acttaaaaaa actgcacaaa ttttgtattt
    12901 gttatatctt cttatgtttg cagtatttgc agtaatatta tattttgcag gtatgccttt
    12961 ctttgatagt attatcatag cgatgggaac tgcaggtaca ggtggttttg cagtttacaa
    13021 tgatagcatt gctcattata atagtcctct gataactaac ttagtttcta ttggtatgct
    13081 tatcttcggg gttaacttta atctttacta tcttctttta ctccgaaaaa ttaaagcatt
    13141 ctttggtgat gaggaactaa aaacctatct ccgcatcgtg gcaatagcaa ctttcatgat
    13201 tgctctaaac gtcatcggta tgtatgataa ttttcgacaa ggcctcgaac atattttctt
    13261 tgaagtatca gctattatta cgacaactgg ttttggcgtg actgatatca ctcgttggcc
    13321 tttgttttca caggttattt tgctattctt gatgtttatc ggtggttctg ctggctctac
    13381 agctgggggc tttaaagtca tgcgtagctt aatcctagca aagattgcac gtaaccaagt
    13441 attgtctaca ctctatccta atagagtcat gtcactacac attaataaaa gcgttctaga
    13501 taaaaataca caacatggtg ttttaaaata tctaactatc tatttagcta tcttcatggc
    13561 actcgttctt gttttaacgc ttgatactaa tgattttttg gttgttatca gtgctgcagc
    13621 ctcatgtttt aacaacatag gcccactctt aggtagcaat gaaactttct cattctttag
    13681 tccattctca aaactccttc tttcatttgc tatgatagca ggacgtttag aaatataccc
    13741 tgttttattg atgtttattc ctaaaacatg gtcaaaacta taattacaaa taataaaacg
    13801 gatatatcaa tctgatatat ccgttttatt acgtcttaag ctaaaatgat cagggacagg
    13861 atcatttcct ccgtgggcta agggatgaca tcgcaaaata cgtgcaatcc ccatcaacac
    13921 accttttaga ccatgttttt gaatagcttc tatcatatac gtagagcaag ttggacgata
    13981 acgacagcta gctgggaaag ctggagaaat atttttttgg taaaagcgaa ctaagaaaat
    14041 caaaaaagat ttaagcatta agatttatta atagccgcat tatgcaactg actaatttct
    14101 tttttattta atcgtcgtgc ttccccagga cggagacccg ttaaatctaa ggtaccgaat
    14161 tgtgtacgag atagcttgtc cactaataac ccaacttgtt caaacatttt cttaacttga
    14221 tgattacgac cttcatgaat agttaattca actactgaac gatttttttc atgatccact
    14281 tttatgatag tatagcgagc aggttttgtt ttcttaccat cgataacaac gccacgcgtt
    14341 aatggtcgaa ggttttcttt agtagctatt cctttaacac gtgctaaata aactttatca
    14401 atttcattac gaggatgaat cattttatca gtaaaatcac catcattagt caaaattaaa
    14461 agtccagttg tatcccaatc caagcgacca acaggatata tccgttcttt gacttgtggc
    14521 aacaagtcaa taactgtctt acgtccttta tcatctgaga cacttgaaat aacaccacgt
    14581 ggtttattta agagataata taccttctct tcgttgtaaa ttggacttcc ttcaatttca
    14641 accagatcac cagcctttac ttgggtcgcc aattcattaa ccacttgacc attaatcgtg
    14701 accattcctt gcttgattaa ttcctcagct tttctacgac ttgcaatccc tgcatgcgca
    14761 atatatttat taattctcat tgttttcctt atcttctgta ttaattgaat ccattgaaaa
    14821 taacgatact tcttcgtcaa ctaattcaat tgaagaggcg tcaattaagt catctaactg
    14881 attaattccc atataatcta agaaataatt agttgtctca taaaggttag gacgtcccaa
    14941 cacttctttc tttccagcct ctttaattaa accaaaagct atcagtctcg ttatagctcc
    15001 actggaattc acgccacgaa tatcatcaat ttcaatccta gtaataggtt gtttataagc
    15061 aataatagat agaacttcca aacttgcccg agatagactc tgattaatcg gagccttcgc
    15121 ataatctctc aacaaagtca tataactatc ctttgttacc aatttataag tttgtgctgt
    15181 ctcaagcaat aatagactag aatcgtcatc ttcttcatat cgtttagcca acttttctag
    15241 ttgctgtatt aatgctgatg gtgtcaaaga caataattct gccatttgac gcaaactcag
    15301 tccatcttca cctgcaacaa aaagaagagc ttctattgag cctaaataag tcatttctct
    15361 tcctttctta agataacttg actaaaatta ctgtcttgtt ctactgtgat ttgatgtaat
    15421 tttatcaact ctaacattgc caaaaataaa gttatcattt cagattttgt ttggcaatcc
    15481 gcaaacactt cttgtaatgt cgttttcttt ttaagattaa aatgtctttc aataacgatc
    15541 atcatatctt caatacggta gtcttctttc tcaattaccg tttgactatt tgacaattct
    15601 ttttgttttt gggacatcat ttgagaaaaa gtcaaaaaaa gatccatcac cgatttatcg
    15661 tgtagaagaa ttgcatcttc aaagataact tcttgttttg gttttgaaaa atatttggcg
    15721 cgttcctgat gctgattagc taactcttca cttagcactt taaaacgtct atattcctct
    15781 aattggctca acaattccat ttctggatca tcttcaatag gatttgactc cactacttta
    15841 ggtaataagt tacggctttt tatcaacatc agctgacttg ccataagcat atactcacca
    15901 gctacttcta gacgcatcgc ctgtaatgtt gctatataag caaggtactg ctcaataact
    15961 tccacaatcg gtacatcgta tatatcaacc tcatatttag atacgaggtg caagagtaag
    16021 tctaggggcc cctcgaaatc ttttaactta atatccatta tctataatat ctctctaaag
    16081 tgataggagt ttttaagcct agaaggcgac tgagctccgt catcgttttg ccattttctt
    16141 tttgctttag aataaattgt tctcgcagaa gttgtgcagt tagttgttgc tcattcttct
    16201 catttaagaa atctgttaat tgattaaaaa accattgtct agaataaggc ttaccgttat
    16261 gctcaaagac taagtcacca tcttcctcta aagattccaa aagaaaagga agcaagtctt
    16321 ctggaagttt taaaatacgc ttcatctgcg atttttcaat acttaagata ttaaaagttg
    16381 tatcaaaatc agccttcttc aaattagcaa tttcagccgg agttaaacct aaagataaaa
    16441 tcaacaaagc aatcaaacga ccactcggat aatcagtatc ttgatagaaa tttgataaat
    16501 ccattatttg agactttgtc tgagccaaag tgattttttc agtctcttgt agtctgtaaa
    16561 attccttcag cgttcctcta ttatataaaa agaaaagaaa ttgattgact gcagataact
    16621 ttcgcttacg agctgttaac ttaaattcac ttagagactg ttgatacaat gccagttttt
    16681 cagaatttac atgtccacca attatttt
//
LOCUS       DATROY010000033        16524 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.033, whole genome shotgun sequence.
ACCESSION   DATROY010000033 DATROY010000000
VERSION     DATROY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16524
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(8..78)
                     /locus_tag="VBD11_000877"
     tRNA            complement(8..78)
                     /locus_tag="VBD11_000877"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Cys,seq:gca)
     gene            complement(212..1744)
                     /gene="ahpF"
                     /locus_tag="VBD11_000878"
     CDS             complement(212..1744)
                     /gene="ahpF"
                     /locus_tag="VBD11_000878"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6953842.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDATYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(1762..2322)
                     /gene="ahpC"
                     /locus_tag="VBD11_000879"
     CDS             complement(1762..2322)
                     /gene="ahpC"
                     /locus_tag="VBD11_000879"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6953843.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            2599..3369
                     /gene="rpsB"
                     /locus_tag="VBD11_000880"
     CDS             2599..3369
                     /gene="rpsB"
                     /locus_tag="VBD11_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6953844.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            3463..4503
                     /locus_tag="VBD11_000881"
     CDS             3463..4503
                     /locus_tag="VBD11_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6953845.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(4662..5123)
                     /locus_tag="VBD11_000882"
     CDS             complement(4662..5123)
                     /locus_tag="VBD11_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6953846.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
     gene            5296..5760
                     /locus_tag="VBD11_000883"
     CDS             5296..5760
                     /locus_tag="VBD11_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6953847.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            5757..8204
                     /locus_tag="VBD11_000884"
     CDS             5757..8204
                     /locus_tag="VBD11_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6953848.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(8424..8915)
                     /locus_tag="VBD11_000885"
     CDS             complement(8424..8915)
                     /locus_tag="VBD11_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6953849.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(8929..9570)
                     /locus_tag="VBD11_000886"
     CDS             complement(8929..9570)
                     /locus_tag="VBD11_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6953850.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            complement(9691..10668)
                     /gene="dusB"
                     /locus_tag="VBD11_000887"
     CDS             complement(9691..10668)
                     /gene="dusB"
                     /locus_tag="VBD11_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6953851.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            complement(10652..11527)
                     /gene="hslO"
                     /locus_tag="VBD11_000888"
     CDS             complement(10652..11527)
                     /gene="hslO"
                     /locus_tag="VBD11_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6953852.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            complement(11667..12923)
                     /locus_tag="VBD11_000889"
     CDS             complement(11667..12923)
                     /locus_tag="VBD11_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6953853.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            complement(12920..13738)
                     /locus_tag="VBD11_000890"
     CDS             complement(12920..13738)
                     /locus_tag="VBD11_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953854.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            complement(13862..16114)
                     /gene="gshAB"
                     /locus_tag="VBD11_000891"
     CDS             complement(13862..16114)
                     /gene="gshAB"
                     /locus_tag="VBD11_000891"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6953855.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDNQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            complement(16284..>16524)
                     /locus_tag="VBD11_000892"
     CDS             complement(16284..>16524)
                     /locus_tag="VBD11_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6953856.1"
                     /translation="QEEPVEVLSGNIQDIISEIVDPSLLNQNIHLSQEVKVEIVSKLH
                     EKGVFQLKGAVSKVAEVLNISEPSVYRYLKKIETH"
BASE COUNT         5150 a         2911 c         3111 g         5352 t
ORIGIN      
        1 atatttaagg cggtagacgg atttgaaccg acgatcaagc ttttgcagag ccgtgcctta
       61 ccacttggct ataccgccat aacagataat attctatcga aaaaaaaaag attcgtcaag
      121 tctctttcgt aaaatagcca attttagccc ttaaacttcc tcatattacc aaaaaaagag
      181 attgatacaa tgggtatcaa tctcttttag attattgtct gattaaataa tcaaatgcgc
      241 caatggcagc tgtggcacca gaccccatgg aaataatgat ttgcttgtag gtagcatctg
      301 tacagtcacc tgcagcaaaa ataccaggga tatttgttga gccaaattta tcaacgacaa
      361 tttcttgacg ttcattgagt tcaatgccgc tatccttgag ccaagaagta cttggtacaa
      421 gaccaatttg tacaaaaaca ccttctaaat caatgtgttt ttcttcgttt gtatcacgat
      481 cggtatagtt taaaccagtg acatgatctt caccaacgat atctttagta gcaacattct
      541 taagaatagt taaattgtca gttttagctg ctcgttcttg cagtacttgg tctgctttga
      601 gctcaggtaa gaattctagg actgtaacat gtttagtgac accggctaga tctaaagcag
      661 cttccatacc tgaattacca ccacctataa cagcaacatt ttttccttcg aaaagagggc
      721 catcacaatg tggacagtaa gtaacacctt tattgcggaa ttcttcctcg ccaggaacgt
      781 tgatatttcg ccacttagca cccagagcta ggatagcagt tttagcttgt aaaatagctc
      841 cgttagccag tgttacctca actaattctt ttttctcaat gccagttgct aattgactct
      901 tgataatatc aatatcgtaa gatttggtat gctcttcaat ttgagccatt aatttagggc
      961 cttcggtgta cagtgtacca atcatgttct caataccaac tgtttcgata acttgtccac
     1021 caaaagtttc tgctaaaata cctgttttta accctttacg agcggcataa attgcagcac
     1081 tgttacctgc aggaccacca ccaataacta gaacatcata gacacctttg tcagcaaatg
     1141 cttcagcatc aagaggtcca tctagttgtt ctaaaagctg ttcaattgta gcacggccag
     1201 aagtaaattc ttcttggtcc ttataaacag taggaacaga catgatacct tttgatttga
     1261 cttcgtcttg atacatgcct ccttcaatca tagtatgagt gatatttgga ttcaaaacag
     1321 ccattatatt aaaggcttga acgacatctg gacaattatg gcaagttaat gaaacataag
     1381 tttctagatt gatagttttc tcaatcccct taattcgttt gataatatct tcatcaactt
     1441 ttggtgcgcg accagagact tgcaacaaag ctaaaatgaa agatgtgaat tcatgcccca
     1501 ttggcaagcc tgaaaaaata acgcgacttt cttgcccttt ttttgcaatc ccaaaactag
     1561 gttgacgttt taaatgtgtt gattcaaggc tgatacgatc agacatagcg acaatttcat
     1621 ctagaaaatc ctttactttt tgagaattat cgttatctcc taaatcagtt tgtaatacaa
     1681 tatcagattc tagcaaatct aaatattgcg ctaattgagc cttgatttct ttatctaaga
     1741 ccatggatta cctcctggtt attaaatttt acctacaagg tctaaactag gagttaaagt
     1801 ttcagctcct tctttccatt tagctggaca aacttcacct ggatgttggc ggatgtattg
     1861 agcagcacgg actttatcaa tgagagtact tgcatcacgt ccgatgccat cagcattaat
     1921 ttccatcatt tggatgacac cgtctggatc aatgataaat gtaccacgtt gtgctagacc
     1981 atcttgtcca aggacatcaa aaccttgtga aataaggtgt gaagggtcac caatcatagg
     2041 atatgtgata gtaccaacga catctgagtc atcatgccat gctttatgaa caaaatgagt
     2101 atcagtagag acagaataaa cttctacatc aagagattta agtgtttcgt attgctcttg
     2161 aaggtcacca agttcagttg ggcaaacaaa tgaaaagtct gctgggtaga aacaaaaaac
     2221 tgcccatttt cctttaacgt cttcatttgt aacagtgatg aattttccat cgtgataagc
     2281 ttgagctgaa aattcaataa tttcttttcc gactaatgac ataataatgt cctcctttat
     2341 tttttaatag cctaattata aacgaaatac ttttaaatct ccaataaaaa cgtttacatt
     2401 tttctaagaa aagtatgatt tttctaatag agtggaagat tttatagctt gtcaaatcta
     2461 actttttttg ataaactact atagttgctt aaagtgacaa atactcatct tagatcaatt
     2521 gtggtcttgt tgccgaaagg ttaggctgca agtcgaagtc tgagagagga aaaaacattt
     2581 ttaaaggaga acttactcat ggcagtaatt tcaatgaaac aacttcttga ggctggtgtt
     2641 cactttggtc accaaactcg tcgctggaac cctaagatgg ctaagtacat cttcacagaa
     2701 cgtaatggta tccacgttat cgaccttcaa caaactgtaa aattagctga ccaagcttac
     2761 gaatttgttc gtgatgctgc agcaaacgat gcagttatct tgtttgttgg tactaagaaa
     2821 caagcagctg aggctgttgc tgaagaagct aaacgtgctg gtcaatactt cattaaccac
     2881 cgttggttag gtggaactct tactaactgg ggaactatcc aaaaacgtat tgctcgtttg
     2941 aaagaaatca aacgtatgga agaagaagga acttttgaac ttcttcctaa gaaagaagtt
     3001 gcattgttaa acaaacaacg tgcacgtctt gaaaaattct tgggtggtat cgaagatatg
     3061 ccacgcatcc cagatgtaat gtatgttgtt gacccacata aagagcaaat cgctgttaaa
     3121 gaagctaaaa aacttggtat tccagttgta gctatggttg acactaacgc tgatccagat
     3181 gatatcgatg ttatcatccc agcaaacgat gacgctattc gcgccgttaa attaattact
     3241 tctaaattag ctgatgcagt tattgaaggt cgtcaaggtg aagatgctga cgttgacttc
     3301 gctcaagaag cacaagcaga ttcaattgaa gaaatcgttg aagtagttga aggatcaaac
     3361 aacgactaat atttaattta ttgtaaatcc taaaacgggg cagatgcaaa ccgctctgtc
     3421 tcgttttttt aatcattatt tgaaattaag aggagaacga aaatggcaga aatcacagct
     3481 aaacttgtaa aagaattgcg tgaaaaatca ggtgcaggcg ttatggacgc taaaaaagca
     3541 ttagtagaaa ctgatggtga ccttgataaa gcgattgaat tacttcgcga aaaaggtatg
     3601 gctaaagcag ctaaaaaagc agaccgtgtt gctgctgaag gtttaacagg tgtttatgtt
     3661 gatggtaacg ttgcagcagt tattgaagtt aacgctgaga ctgactttgt tgctaaaaac
     3721 gaccaatttg taacattagt taatgaaact gctaaagtta ttgcagaagg acgtccttca
     3781 aacaatgaag aagcacttgc tttaacaatg ccttcaggtg aaactttaga acaagcattt
     3841 gtaactgcaa ctgctacaat tggtgaaaaa atttcattcc gtcgcttcgc tttagttgaa
     3901 aaaactgatg agcaacattt tggtgcatac caacataatg gtggacgtat cggagtaatt
     3961 actgttgttg aaggtggcga cgatgcactt gctaaacaag tatcaatgca cgttgcagct
     4021 atgaagccaa ctgttctttc atatactgaa cttgatgcac aatttgttca tgatgaatta
     4081 gcacagttga accacaaaat tgagcaagac aacgagagtc gtgctatggt taacaaacca
     4141 gctcttccat tccttaaata tggatcaaaa gctcaattga ctgatgaagt tattgcccaa
     4201 gcagaagaag acatcaaagc agaattagct gcagaaggta aaccagaaaa aatctgggat
     4261 aaaatcgttc caggtaaaat ggatcgtttc atgcttgaca acactaaagt tgaccaagag
     4321 tacacactcc ttgctcaagt ttacatcatg gatgatagca aaacagttga agcttatctt
     4381 gagtcagtaa atgctaaagc agttgctttc gtacgttttg aagttggaga aggtattgag
     4441 aaagcttcta acgactttga agcagaagtt gcagctacaa tggcagcagc acttgaaaaa
     4501 taattaacac tcgctttata ttataatttg gcagcaaaat gttgtcagta aatatatttt
     4561 aaaataagac acttggctta agtcaagtgt cttattttgc tatcgaattg gtggattggc
     4621 aaatattaaa aaggagagac agtcatctca ccttaacttt tctagaatag gttgaagccg
     4681 ataatggata atgttcccat gatgcatcca gctagtataa cacctagcat aacagcaata
     4741 acactatgtt taaattccca atctagaagg ctagctgcaa gtgtgccagt ccatgctcct
     4801 gttccgggta agggaatgcc tacaaacaat agcaaggcaa taaacaatcc tttttctcca
     4861 gctacttttt caagtttttg accaccgcta tgtccttttt taaggcacca tgtgaaaaac
     4921 ttaccagtat aaggtttatc tgctccccat tctagaactt tgcgagcaaa gaagaagatg
     4981 ataggtacag gtagcatgtt tccaacaaca ccgatagcta gcgcttccca caaaggtatt
     5041 ccattagcga ttgcaaatgg cacagctcct cgtagttcca ctaagggaat cattgaaatc
     5101 aaaaatgtaa taatgtaatt cattaaataa aacctttcta tggtattatt ccattttatc
     5161 ttatcttgct agtaatgaca actaaaatcc gcggtcattc tagttttact atccttagat
     5221 agatttcatt tccgtatctt ttaaaaattt gatataataa tagtcagtta tagtcaaaga
     5281 ggaaggagtg aatttgtggc cataaaaaat acttccgata atattgaaga atatataaaa
     5341 tcattattag aacaatcagg aattgctgaa ataaagcgtt ccaatttagc tgatactttt
     5401 caagttgttc ctagtcagat taattatgtc attaagacac ggtttactga aagccgtggt
     5461 tatgtggttg agagtaaacg aggtggtgga ggttacatcc gcattgctaa ggttcatttt
     5521 tctgaccaac accaattatt tggaaatatg ttatcaacga ttggcgagcg tatcagtgaa
     5581 caagtctttg atgatttgat tcaattatta ttcgatgaag agataattac agaacgtgaa
     5641 ggaaatttga ttctggcgac atcaggtgat gacgtcttag gagagcaagc ctctgttatt
     5701 agagctcgta tgctccgtaa attattacaa cgattagata gaaaaggatt ccatttatga
     5761 gtcattattc cataaaatta caagaagtat tcagattggc gcaatttcaa gcagcacgct
     5821 atgaaagtca ctatttagaa agctggcatc ttcttttagc aatggtttta gttcatgatt
     5881 cagtagctgg tttgacattt gctgaatatg agtctgaagt tgcaattgaa gagtacgaag
     5941 cagcaacaat tttagcttta ggtagagcac cgaaagaaga gattaccaat tatcaatttt
     6001 tagagcaatc accagctttg aaaaagatct taaagttagc agaaaatatt agtatcgtag
     6061 taggagctga agatgtgggt actgaacatg tcttgcttgc tatgttggtt aataaagatt
     6121 tattagccac acgtatctta gaattagttg gctttagagg tcaagacgat ggtgaatcag
     6181 ttcgtatggt tgatttacgt aaggctctag aacgtcatgc cggttttaca aaagatgata
     6241 tcaaagcaat ttatgaatta cgtaatccta aaaaagcaaa gtcgggtgct tctttttctg
     6301 atatgatgaa accaccaagt actgctggtg atttagctga tttcacacgt gatttatcac
     6361 agatggcggt tgatggggaa attgaacctg ttattgggcg tgataaagaa atttcacgta
     6421 tggtccaagt cttaagtcgt aaaacaaaga ataatcctgt cttagttgga gatgcgggtg
     6481 tcggtaaaac agctttggcg tacggtctag cacaacgtat tgctaatgga aatattcctt
     6541 atgagcttag agatatgcgt gttctagagt tagatatgat gagcgttgtt gctggaactc
     6601 gttttcgtgg tgattttgag gaacgtatga atcaaatcat tgcagatatt gaagaagacg
     6661 gtcatattat tcttttcatt gatgaactgc atactattat gggttctggc tctggtattg
     6721 atagcacact tgatgctgct aatattttaa aaccagcttt ggctcgaggc actttacgca
     6781 ctattggagc aacaactcaa gaggaatatc aaaagcatat tgaaaaagat gctgctttat
     6841 ctcgtcgctt tgcaaaagtt ttagtagaag aaccaaatct tgaagacgca tatgaaattc
     6901 tacttggatt gaagccagcg tatgaagcct ttcataatgt taccatttca gatgaagcag
     6961 ttatgactgc tgtaaaagtg gctcatcgtt atttgacaag taagaattta ccagactcag
     7021 ctatagactt acttgatgaa gcttctgcca ctgtgcaaat gatgattaag aaaaatgctc
     7081 catcgctttt aacagaagtt gaccaagcta ttttagatga tgatatgaaa tctgcttcta
     7141 aagctctaaa agcaagtcat aaggataaaa agcataagcc tattgccgta accgaagatc
     7201 atattatggc aactctcagc cgtctatctg gtattcctgt tgaaaaattg acacaagctg
     7261 atagtaaaaa atacctcaat ttagaaaaag aattgcataa acgtgttatc ggtcaagatg
     7321 atgcagttac tgccattagt cgtgctatcc gtcgcaatca atctggcatt cgtactggta
     7381 agcgtcctat tggttccttt atgttcttgg gaccaactgg tgttggtaag acagagttag
     7441 caaaagcctt ggcagaagtg ttgtttgatg atgaatcagc cctaattcgt tttgatatgt
     7501 cggaatatat ggaaaaattc gcagcttctc gcctgaatgg ggcacctcca ggttatgttg
     7561 gttatgatga aggaggagag ctgactgaaa aagtacgcaa taaaccatat tcagttttac
     7621 tttttgatga agttgaaaaa gctcatccag atatttttaa tgtattatta caagtgcttg
     7681 atgatggcgt tttaacagat agtcgaggac gaaaagttga cttttcaaat actataatca
     7741 ttatgacttc taatttagga gcaactgcat tacgtgatga taaaacagtt ggttttggag
     7801 ctaaggatat cagccatgat tatactgcta tgcaaaaacg tattatggag gagcttaaaa
     7861 aagcttatcg accagagttt atcaatcgta ttgatgagaa agttgtattc catagtctca
     7921 gtcaagataa tatgcgtgaa gttgtgaaaa ttatggttaa acctttaatt ttagcgctta
     7981 aagataaagg tatggatctg aaattccagc cttcagcctt gaaacacttg gcagaggatg
     8041 gctatgatat agaaatgggt gctcgtccac tgcgtagaac tattcaaacg caagtagagg
     8101 atcatttatc agaactacta ttggcaaatc aagtgaaaga ggggcaagta attaagattg
     8161 gcgtatccaa aggcaaattg aaatttgata ttgcaaaatc ataaaacatt ccagtaccga
     8221 tgggtactgg aattttaata taaaaagaaa atgtccaaaa tattttggac attttcttat
     8281 agatttatga aaagtaaaag gatctatatc taacaattgc acttgtagat ataggtacga
     8341 ctgaaagtcg ttgaaaaatt ttaggataaa tatattataa tatcctaatc ttttgctaac
     8401 ttaaaataaa tctaaagatt accttaagat ttcaatcgct tttgtaacca tacaatgtca
     8461 tgccattgtt caaatttaaa cccgattttt gggaaatgtg cgacttgatt aaatcctctt
     8521 tttttgtgga atgcaatgct tatatcattt ggtaaactga tacaagcgag tagattaaca
     8581 tacccttggt taactaattc tttctccaat gtatcatata gttggctacc aatttttttg
     8641 ctacgtacat ctttatcaag gtaaatagaa agctcgcaag tccagtcata ggaagctcta
     8701 gcgtagtaag ttgaagcata tgcataacct aatattttcc cattttcttc agcaactaaa
     8761 tatgggaaag atttgctgat atttttgata cgattagtga attccgatat actaggaata
     8821 tcgtattcaa acgttattgt agtgtcagta acgtagggag catagatagc aagtaattct
     8881 ttagcatctg agggtaggac aggtcttata atcatagata tcacctaatt atagtaaatc
     8941 aagtttttgc aattcggaat caatagtgtc aaggacgtgt tgaagatcct ctggattttt
     9001 gacgaaatca agtttgttac catcgatccg aattttaggt gaaacgtcgt aattttcata
     9061 ccattctgga tattcagagt ggacttgttt gtagtagtcg tataattctg ggttagaatc
     9121 tacttgctca aagctgcgtc ctcgtttgtc aatacgttct aacattttat caaatgaaac
     9181 atcaatgtag actagcaaat caggtctctt cttaggcata ccttctaatt cttccaacat
     9241 atttgcaaga agttctttat agatatctag ctctgtttta gtcacatttc cattcttaaa
     9301 atttagagtg aggaatagtt catcttcaaa aatagaacga tccaaaacat tgttgttagc
     9361 cttataggct tcttttatag attggaatcg tttatttaaa aagaatattt gtagtaaaaa
     9421 agcatatttt ttgggatctt gatagtaaag atctaaaact gggttattat caaccgcctc
     9481 atagaaaaca tctgtcccta ggtgttgtcc tagagcagct gcaagggagc ttttacctgc
     9541 tccaatggtt ccagctaaaa caatcaacat aattctcctt aattctgtta tactaaaata
     9601 atttgttata cttttgagtt ttcattacac aatgatttat gctaaatttg tacagagata
     9661 ggaaaaaaca gtcaaaggac tgtttttgat ttagcgtaaa cctgcaaaaa gttcttgaac
     9721 ttctgctagt gtttctgcac gtgaaacagc tcctctaatt tttgctgcgc ctgattttcc
     9781 acgtagataa tgaggtgcta accctcgaaa ttcacgaacg gcaatagttt caccttttaa
     9841 attaactagt cgagtgagat gatcctcggc aacatctaac atcttctcaa atggtaggtc
     9901 gggaaggatt tcgcctgttt caaagaaatg attaatttgt gtaaagatgt aaggattgct
     9961 acgagcacca cgtccaacca ttatagcgtc agctccaatc tcttcaatca taaatttggc
    10021 atcgtgaaca gtacgaatgt caccattggc aataaaagga atactagtaa cagctttagc
    10081 gaccttgcca agtgtttcat ggtcacacgt gccagtatac atttgttcac gtgttcgacc
    10141 gtgcatagca agtgcagata caccagcaga ttcggcagcg agtgcatttt ctacagcatt
    10201 agatgagtca gaccatcctg tacgcatttt tactgttaat ggaatgtcaa gtacagacgt
    10261 tacctcttta acaatatgat aaattttttc agggtcacgt aaccatttag ctccagcttc
    10321 gtttttaaca actttgttaa ctggacatcc catattgatg tctacgatat ctgctttcgt
    10381 attattttgg ataaagtcag ctgcacgttt taagccttct gcatcgccac caaatagttg
    10441 aattgacata ggatgttcat tttcatcgat atggagcata tgtaaggtct tttcgttgtt
    10501 ataaagtaac cctttctcag aaatcatttc cataacaacg agaccagcac caaattcttt
    10561 agcgatagta cgaaaagcag aattagtaat acctgccatc ggtgctagta cggtacgatg
    10621 aggaatttca accttcccaa tcataaatga actatttaat tgagtcatta attatcttct
    10681 ccaaatcttc ttctgtgaaa tagtaagtct ggttgcaaaa ttgacaagta atttcaacac
    10741 ctttatcttc atccttcatg gcttgcaatt ctttagttcc taaagtcaat agagcggctt
    10801 caaaacgttc ttttgaacag tcacaataga aggctaaaga gtcttccgat aagcgcttgt
    10861 agtcgtcttc tccgtaaata gctgaaagga gagattctat atgattttca gactctagca
    10921 gacttgaaat agaaggcatc tcctgaatgc gtttttcata gcgagaaatt tcttcgtcag
    10981 aagcacctgg tagcacctga agcatgaatc cacctgcaac tttaacctta tcctcgtcat
    11041 ctaagaggac atttaaaccg acggcagaag gtgtttgctc actttcggtt aaataataag
    11101 caaaatcttc tccaatttct cctgtaatta gtggagttgt tgaagtgtaa ggttgcccag
    11161 ttccgtaatc ggtaataacg acaaagtgtc cattccccat gaaaggtcca actaagacct
    11221 ctccagtagc tgttttcttg atatcaacac ccgtattttg gatgtaacct ttaacatttc
    11281 ctttagtatc agctacagaa ataatatgac caaaagagct atctccaata accttgacag
    11341 ttactttact atttcctttt tgatttgctg ctaaaatttg atttgcaatt aatgttctcc
    11401 caagagctac agtagaactt gaaagtgtct gatgtttttc ttgagctgtt cttacagttt
    11461 ctgtacaatc aaggacatag gcccggaaag aaccggaagt tgaaattgat tttataattt
    11521 tatccataag tcatattata gcataaaagg cttattttgc attggagata gtcaggaaaa
    11581 tatagatgaa aacggtataa attaatgata taaaaacgat actgtgtttt acagtatcgt
    11641 ttttatatag tggatatgat agtaagttaa gactcatcaa ctttttcttg ggtagattta
    11701 ccttcgttga gtaaggcttc aactattttt ttatcacgaa ttttgacaga atcattgatt
    11761 gtttcagccg atttaataac agcagattct aattgggcac gttccttacg tcctttgtct
    11821 atggcagcga taataccatt attttgagcc actaagcttt cagctaatgc agtgacagat
    11881 ttaatagaaa cagtggggct ttgtgcggtc ttctctaaca tcggaatcgc ttctttacta
    11941 gtttcagcca gcatttgcaa tgctgcatta ttagcgttga caatagcatc agcagtgaca
    12001 ccggatttga cagattgttg catcatgcct aactgagcga ttgagagttt cattgttgga
    12061 atggtatttc gacgtaacat accaagtttc tgacgcatat ctgacgatac tttgaccaag
    12121 tttcgcatct gtggtgttgt tgcccatgca acgtagagac ggctgacata ttccgtatgt
    12181 tgctgttcga gggtattgat aacttcagtc attcgggcta attggttact tttaatttga
    12241 tattcggacg tttggctatc taatgctaga atttcttgtt gtaagtggct tgcacgattg
    12301 gcagcctcgg cttgactcga ttcaataaaa gcaataactc caaccaaatt ttcaatagat
    12361 ttagtattat cttcaatgag catttcagca gagacgatat ttcttgccaa agtatcttct
    12421 tgtttgacaa catttgctgc catcatatcc attttttgct cgatgttttg tgagtcaaaa
    12481 taaaattcct gtagcgaggt cttgctttgt ttgaataatt tttgaatcaa gtttggtttt
    12541 ttctctaatt ctgccggagt agcatcttta tatttggcaa taaatccatt tagttcgcga
    12601 ttagcatttt ttagtaaatc atcaacttga ggaatttgaa tttttttctg ctcagacaag
    12661 atatgattaa cagtagtatt aacgccttct actgcggatt gtccaaaatc aaggagcgca
    12721 ttttggtcac cgacaaaagt atctaccaaa gctggtgttt tttcagagat agcagacttt
    12781 tgtgctggtg ttagtttttc aaaaaaggca atttgcccag tttggcttgt tgtctggttg
    12841 gaaataattt ctgttgtttt atctgtttta gtgatagcat tgtctgcaat ttggtcaata
    12901 tcaaaattaa aggtatcgct catttattgt tcctttcgtc ttgcatcatt cttaggctaa
    12961 cgtcaaaatc tttcatatca ctttcattca attggcgcag cgtttcatct aaatctaaat
    13021 taaatttttt aattgcttgt aatgcttgtt ctaggcgttc cttagcattg ttaaaatttt
    13081 taggagattg tttgattctg atgtagccag ccagaacatc tgtaaaacgt ttcatattgg
    13141 cttgatgaat tgctgctaat tcagctttgt tatctgcctc ttcaatcttt tttaagatag
    13201 catagtgatc atcttgaata ttgtgataga acggttttaa ttccggagca atgcgtatca
    13261 tttcatcttc ctgatcagaa ataggttcta gttctggaga ggcatgtagc tttattaatt
    13321 gttcgttaat ctcattacta aatgtatttg cttttttggt gatacgttta tagatattag
    13381 gctcaatggc tttttgtagt tggtttgctt cattacgaat taggtccaat tgaggtaata
    13441 tttgatgtgc taatttgata tattccttag aacgattgtc tttttttaaa atttccaagt
    13501 gtctaatgtc ttggtcagtc tctttaatat cggattttaa agcttcaata cgttggtttg
    13561 atgtaaaaca tttaagacgc tttttatcat aaacgtaata ggcacaataa ccaccaccaa
    13621 taattaacag agtagcaatc acctcactcc aaaagtctga aatcagggaa gcaagaacaa
    13681 caataccaag taatgttatt agattatgag aatttttgga atagcgtttt ttaaccataa
    13741 taatatttaa cctaactttc tgtagttgtt aatattttat cttaaatgaa ctagtgatga
    13801 caagggataa ctttatttta accaaaaatt ccttaagctt agcctaagga actcttttta
    13861 gttataattc tgggaacagt ttagccaaaa ttcgaggtgt aatagattga ccaggcccct
    13921 cttgacagta ggtgtgcata tacatcaatg ggttaaagtt tagttcaatg caagtagcat
    13981 ttttcttatc ttttgagtat gcttgagtgg cattaggaat aattaaatca actccacaaa
    14041 cccaagcccc catagcctct gccatctcag ctgctagttg tttatatgta ggatccattg
    14101 tatttgttac atctatagag tcaccgccag tggaaatatt agaattacgg cgaagttcta
    14161 tttttgttcc ttctggaggg atactgttaa cagtgtatcc ttgttgttcc aacattaact
    14221 gttctacttc tccgagctca ataacctcta gaggagagcg atgatcatag cctctgagag
    14281 ggttttggtt tttaagttta actagctgac taatagtatg aataccatct ccaacgacat
    14341 tagctgctac cctaagcaat acagcaatac agtccccctc aaggacgaag aaacggtatt
    14401 cggtaccctc aatatattct tccacaagaa tagcgctatc ttcggcaaaa gcaatgtcaa
    14461 ttgctttttc ataagaagct aagttagctg atgttttaaa aatagaaatg cctaaaccaa
    14521 agtttgtaga ttttggctta acgacgattg gtttatcttg gatttgtgaa aaatagttaa
    14581 gtgcctcttt acggtcagta aattcatctc caaaaggagt tgggaaatgc ttttcatcta
    14641 ggattttttt tgtaacaact ttgttagcca tagcgagcgg tacaatatag ttatcttttg
    14701 aggtcatatt accattttta acgtactcaa tatgactatt gtgccataat tttaggaatt
    14761 gatcttgttc atccagtact tcgaagttga ctcctttttg gataacgtca aatagtagca
    14821 attgggtaga aagttccatc gtttcatatc cctttaaggc gtagggagct tcccaagcat
    14881 aatcgtgata aatttgtcct tgtctttgtc caaatgtttc taaggaaagt ccttcaatca
    14941 tttctaaaag ttgaccagct accgttaatt taggtgactg aatttgcctt tttacagatt
    15001 ccaataggtc ttgatagtaa ggtggtaagt taaaatgttg gataacagat tgcatagcat
    15061 ccactaagtc agaaactggt gcttgattag gtaatttttc taatggatga ctgagtgcta
    15121 taaggtcatt taatctattg gcttccttga tatcttggtc aatatgacta ctagaatcta
    15181 tccacaaaag cgccaataag aaaaggtgaa cagtatcaac agtttcctgc gttataccga
    15241 ttgggctaaa tgggttgaga tcaaaagtgc gaaattctaa atatgtaatt cctttttcta
    15301 aataattacg acaggactta ctaccacgga gacgaacagg tgaataaaat tctttctcag
    15361 caatcaattg cccacttttt acagcatttt ctagatcgtt gacataatcc ttcaagctag
    15421 tataagaaat acgaatatct ttatgattca catagcctaa gtgactattt ctaagtgacc
    15481 ggacagggtt attaagtttc tgatttaaaa agtcttcttc agctactggg ctcgctccgt
    15541 atagataagt tagtaaccaa cggtaacgta aaaaattttg agacaatttc atgtaaagct
    15601 ggttctgaaa atcaatagca ttatctgctt gactcaattc aaagagagaa gttaataact
    15661 cttgacctaa accgagatta tagtgaattc cagagatgga ttgtattagt tttccataag
    15721 ttttctccaa atatttgcga taatcatatt caaaagcatc ctctaattgg gcaatctgta
    15781 tgtcttcttc cctcactttg ggtggcatag aaagcggcca taagtattca tcgtggttga
    15841 tagatcgtcc agcgacatca cttatagctt tcaaaaatcg aatggcttct tggttatcct
    15901 ttgcaatagg tgtaatgagt tctaattgag gttcactata atccgtttgg atataaggat
    15961 gatagttacg actccctaat gttttgggat ggggtgtttg agcaaccctt tgagtgggtt
    16021 ggtgaatacg aaggctttct ctctctaagc caaatgtagc ttgtagaatc ggtagatgag
    16081 agtggcttct ttgtaacagt tgatcgataa tcacgacaac attcctccta gtaacagatg
    16141 atactagttt atcaaaaaaa taataaaaac taagttttac ggcacagaaa actaaaataa
    16201 tcttatactg gtgtcataaa aaataaaaga ttgaactttt gagtccaatc ttattaatct
    16261 ataagttaaa aagagcttta gagttaatga gtttcaattt ttttaagata acgatagacg
    16321 cttggttcgg agatgttgag aacttcagcg acctttgaaa cggcaccttt taattggaat
    16381 acaccttttt cgtgaagttt agaaacaatt tctaccttaa cttcttgact taaatgaata
    16441 ttctgattta aaagactagg atcaacaatc tcactaataa tatcctgaat attgcctgat
    16501 agaacttcta caggttcctc ctgt
//
LOCUS       DATROY010000034        16344 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.034, whole genome shotgun sequence.
ACCESSION   DATROY010000034 DATROY010000000
VERSION     DATROY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16344
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(46..1584)
                     /locus_tag="VBD11_000893"
     CDS             complement(46..1584)
                     /locus_tag="VBD11_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6953857.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(1677..2777)
                     /locus_tag="VBD11_000894"
     CDS             complement(1677..2777)
                     /locus_tag="VBD11_000894"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6953858.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVTSLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(2774..3133)
                     /locus_tag="VBD11_000895"
     CDS             complement(2774..3133)
                     /locus_tag="VBD11_000895"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6953859.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(3158..4165)
                     /locus_tag="VBD11_000896"
     CDS             complement(3158..4165)
                     /locus_tag="VBD11_000896"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6953860.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(4291..6819)
                     /gene="secA"
                     /locus_tag="VBD11_000897"
     CDS             complement(4291..6819)
                     /gene="secA"
                     /locus_tag="VBD11_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6953861.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(6928..7875)
                     /gene="manA"
                     /locus_tag="VBD11_000898"
     CDS             complement(6928..7875)
                     /gene="manA"
                     /locus_tag="VBD11_000898"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6953862.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(7993..8874)
                     /locus_tag="VBD11_000899"
     CDS             complement(7993..8874)
                     /locus_tag="VBD11_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6953863.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(8942..10861)
                     /locus_tag="VBD11_000900"
     CDS             complement(8942..10861)
                     /locus_tag="VBD11_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6953864.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            11046..12485
                     /locus_tag="VBD11_000901"
     CDS             11046..12485
                     /locus_tag="VBD11_000901"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6953865.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRTFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            12487..13449
                     /locus_tag="VBD11_000902"
     CDS             12487..13449
                     /locus_tag="VBD11_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6953866.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(13533..13967)
                     /gene="nusB"
                     /locus_tag="VBD11_000903"
     CDS             complement(13533..13967)
                     /gene="nusB"
                     /locus_tag="VBD11_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6953867.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(13960..14349)
                     /locus_tag="VBD11_000904"
     CDS             complement(13960..14349)
                     /locus_tag="VBD11_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6953868.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(14438..14998)
                     /gene="efp"
                     /locus_tag="VBD11_000905"
     CDS             complement(14438..14998)
                     /gene="efp"
                     /locus_tag="VBD11_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6953869.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(15926..16225)
                     /locus_tag="VBD11_000906"
     CDS             complement(15926..16225)
                     /locus_tag="VBD11_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6953870.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT         5168 a         3218 c         2638 g         5320 t
ORIGIN      
        1 aagatcacca aaaaggcgcc tcaagagcgc ctttattatg tctaattatt tatggccaat
       61 aacataagtt aattgtgcag cttgtgctga actaaaagta cggtaagaga caataccacg
      121 tcccattgca ttagattctg aaatgagaac tgaaccatct tttttaactt cttcaacaat
      181 agctacgtga ccgtaagtgc catcagcacc agcttgtcct ggtgaaaatg atacagcata
      241 gcctactttt ggtgaatgtg ttgtttcaaa gccagcctta tgttgccaat ctccaccatt
      301 acccataaat ggatcaaatg tataacctaa ctctttagcg cggttaaaga cataccatgt
      361 acattcaccc catggataag ttgatgcaat atagctagct gtgttaaccg ctgttgataa
      421 gctaaaacca cttggtaaat ctgctaaact tgctgtcgat aaagctttca aggcagcagg
      481 aatttctggt gtccctccat gttgatcaat ccagcggtct aaatcttcca taatctgggc
      541 acgacgttta ccagttccag agttatctgt ataataaaca ccaccctcag taactgtatt
      601 aagttgtcca gaagataatt tctgtgcttt caaatcttgg atttcaaagc tagagttgtt
      661 gtttttaata attgtatttt ttgtcaacaa cataattgct tcttcatcat tataagcact
      721 agcatggcta gagtcatgat caaaggcacc ataaccaaac atattagcac ctggcatctt
      781 agccactcct tgagttccca atgaactttc aaggacagca atagctacaa tagcacggac
      841 atctaaacca ctatcttttt cccattgtaa taacttagca ccattgatac gattagaatc
      901 atattgaatc cctaaagatt ttaagtaccc atctaattgt tcagctttga taccgtaacg
      961 atgtgacaat aaatagtgag agtaggcatc ctgaccagac cagtgttcta cgtagaatgg
     1021 cgttgctgtc actggtgaaa tcggtgttga taataagtta tcatcaaagg ataaagcagg
     1081 aacagaagca ttctgatttg tctgaacact ttctatctga gacggagcgg gagttatttt
     1141 aggctctact aatggtaagg taggctcagg ttttaattca gtctttgttg attgacctgg
     1201 cttactgtct gcttgactac tgtttgtagt attagtgcta ggagtagatg ctgttgaagc
     1261 agaatcagat gttttagcat cttcgttgga agaagattga tgtgatgaac ttgatgatga
     1321 ttcattgctt gatgatgtct ctgtcgtacc aatctctgtt ttttctgttg agatacttcc
     1381 ttcggctgaa ggtgaagcag tttctgaatc tgtaggttgt gttttttcct gtttctctgc
     1441 ttcagaagaa ctttctgttg atgctgttgc cattgtcagg cttgtttctg gattaatagt
     1501 ttctgctaag gtcactaaag gtgatgatag cgataagact aaagtggata acaacaaagt
     1561 tgccgttact ttttgttttg acatatacta atgattccct tctataaaaa ttgtcactga
     1621 ttagtctaac atatttttaa attttttaaa acagttaact actgtgtcat ttgtttttaa
     1681 taatatctcg gaatacgatc gcttaaaaga caaagtactt cataattaat cgtctgtctt
     1741 ttttgagcga cgtccgtagt tgtaatgtta caactacctt gttgaccaat caaggttact
     1801 ttagttccga tagtatattt ttgaggtaaa cggatagtca tttgatccat cgagacacgc
     1861 ccaataattt cacaaagttc tccatttact ataactgaaa atccttgcat atctcgtgtc
     1921 catccatcag cgtagccaat cggtactgtt ccaacaaatt cgtctccagt aacttgatag
     1981 gttgcgccat agccaacttg agaaccatcg tgtagttgtt taacatgtac tagctcagac
     2041 tctaaactta aagcagggtt tatagggtaa ggtaagtcta aatcagttcc acttggattt
     2101 aagccgtaca taacaacacc caatctaacg gcattaaaaa ttgtctcact atgccaaagg
     2161 cttgtagcag aattactagc atgaacaagg tccggacaat tatctaatcc tgaaataaag
     2221 tctttaaaaa aagtaagttg ttgattgaat ttacagttat cggcttcatc agctgttgca
     2281 aaatgggtga aaatcccctt gacagaggca ccactttccc ccaattcaga aattagctta
     2341 tttccttcat ctaactggcg aacacctata cgtcccattc cactatcaac tttgatatgt
     2401 acttctagac cagataaatc tactgctgat gtctgacata aacgtaacca ctccaagcta
     2461 gtaactgtta gagtaatatt ttcatttttg gctagtataa cctgctcagg cataacaaca
     2521 cctaatacca atatcatttt tacaattcct gcttgacgta gctcaatagc ctcatctaaa
     2581 ttggatacgc aaaaaccatc tacaatagac tcaatgtatt ttgaaacttc aacagcacca
     2641 tgcccataag cattagcctt aactacagca aatgtttttt tatcacttgg aatgtgctct
     2701 tgtacagatt taacgttatt tgcaatcgcc tctaaatcta tcaaggctct ggttggtcta
     2761 tgataacttg aaatcatttt tcttcctcca aaataacact agcttgtgca taattaccac
     2821 tatgagatat tgaaacaaaa gattttccat taaacggttc ttttgtaatc aaaggcgctc
     2881 ctttatcatc cgataaaatt tcgatatcat gaaaattaac tttcccaatt cctgttccaa
     2941 gtgctttgct ataagcctct tttgctgccc atcgccctgt taaaaaagac atctgacgct
     3001 tgggattgga aattccttta aaaagaagca attcttgttc ggtcaaaacg cgttctgcaa
     3061 aacgttgatt acgctcatat gctttagtaa tcgcctctat ctcttgtaaa tcaattccgt
     3121 gtccaacaat catgtgtaac tcctataagt tcgatactta caaactattg taaatatccc
     3181 taattagcat ttctgtttta tcccaaccta gacatggatc tgtgatagac ttaccaaaaa
     3241 catctggttt atcttgtctt ccatcttcta aataagattc tatcaaaaaa ccacgaacat
     3301 agtttctaat tttagtatgc cactgtcggt ctgcaagtac tgatttcaca atgcgaattt
     3361 gttctaaaaa ttgcttacct gaattgtcat gatttgtatc aacaacaata aagggatttt
     3421 gcaaatcagt ctcttcataa tgagtaatag tttctaaaag gttttcataa tgataattag
     3481 ggatacttcg atagtcggca ttatgatatc ctctcaaaat aacatgtgac agcaaattac
     3541 catctgttct aacttgctta ttttgataga ataattcctg ttgattttgt gctgcataga
     3601 ccgcattaaa catgactctt aaattaccag acgttggatt tttcatacct actggtgcat
     3661 ctatacctga agatataaaa cggtgtccct gatcttccac tgaacgcgca ccaatagcat
     3721 agtaagaaac taaatcatcc atcactgata acatctctgg ataaagtaat tcatcagctg
     3781 tcaggagtcc agtctctcga atgatgtcat agtgcatctt acgtgcttga aaaaaaactc
     3841 ctagtttgtc agaatggtga actaagcctt tataaccatc accattcgtt cttggcttag
     3901 cggtataaac tctcataacc aaaaacattt tatctttaaa ggcagcttcc aatttagcta
     3961 aacgctttgc ataagttagc accgcctttg gattatccgc agaacaagga cctactatta
     4021 ctagaaaccg ctgatcctca ccttttatga tattttttaa ctcttcttct ttggctaatt
     4081 ttttttggct aacttctaga tctaagctag ttttctgctt caatatttca atattaattt
     4141 tatcacttaa ctgatgaaat cccatgtttt ttcctaatac aacctaagtc tattaataag
     4201 ctctgctata caatatagtc gtcatactat tagacaagta atgataaaac agtcttctca
     4261 gactgtttta tttttatttt aaaatcattg ttattgtaaa catctcagac catgacaatt
     4321 tttaaacttc atgccagagc cacaaggaca tttatcatta cgtttaatcc cttggtattc
     4381 tggtgattca ttattcatag gtacatgttg tgccgagata ttttgctctg ctgttgtcgt
     4441 agcatgttga gaagcacgtt cacgctcttg ttcatgaatt tgagctttca tcaatgtacg
     4501 tgtcacatca aactcaatcg aaccaatcat atcttggaac atacggaaac cttctgattg
     4561 atactcaacg attgggttat tttgagcata accacgaagt ccaacggagt tacgtaattg
     4621 atctaaggca tcaatatgct ctgtccattg attatccact accatcaaga ttaagacttt
     4681 ttggaattcg ataatagctt ctggatcttt taatttagca atttgttgct cataaacagc
     4741 taaggcacgt tcataaagaa gttctttaat ttcagcctct tttagaccac gaagttctga
     4801 aactgtgata gcttcttcat ctaataacgc cgatcttgca aaattaacaa tagcttctgc
     4861 tgcagtattt ttatcgcttc tgctatgtgc atcaactgct cttttaatcg tacgtttaat
     4921 catccctttg agctctgatc ctaaatcacg ctcagcagta atcacttctc tacggttagc
     4981 ataaataatc tcacgttgtt cacgcataac atcatcgtat tgcaagactt gtttacgtgt
     5041 gtcatagtta ttaccttcaa cgcggcgttg agctgattca acttgtcgtg tcaacatttt
     5101 tgatttaata actgtatcat cttcagctaa attcattcgc tcaagaacca ctttaatacg
     5161 atctgtacca aagcgacgca tcaaatcatc ttctaacgaa agatagaatt gtgattcacc
     5221 tggatctcct tgacgacccg aacgaccacg aagctggtta tcaatacgac ggctttcatg
     5281 acgctctgta ccaataacac ataaaccacc aagttcacga accccttcac caagcttgat
     5341 atcagtacca cgaccagcca tgttggttgc gattgtaaca gcaccacgtt gcccagcatt
     5401 cataatgatt tgagcttctt taaagtgatt tttagcattc aaaacttcat gtggtactcc
     5461 agcagcaact aattttctag aaattaagtc tgacgtttca acagctactg taccaaccag
     5521 tacaggttgt ccttgttcat aacgttcttt gacatctgct accaccgctc taaatttaga
     5581 gtcaagagtt ggataaagta gatctgaatg atctatacgt tgaacaggac gatttgttgg
     5641 gataggaata acgcgcatat tataaatttc acgaaactct tcttcctcag tcttaccagt
     5701 accagtcatt cctgctaatt tatggtacat acggaacata ttttggtaag taattgaagc
     5761 gctcgttttt gattcttctt ggattggcac tgactctttt gcttcaatag cttggtgtaa
     5821 gccatctgag aaacgacgtc cttccatcgt acgaccggta aattggtcca caattaaaat
     5881 ctcttgttct tcactaacca cataatcaat atttaacaac ataatatagt tagctcgaag
     5941 agcgttatca atgtagtgag ttaaggcaac attttcgaga tcatacaagt tatttagatg
     6001 gaaatagttt tcagccttgt caatacctgt atctgataaa ccaattgttt ttgttggaac
     6061 atcaatgata taatcatcgc tattaagcgt ttttacaaac atgtccgcac gtgtatacag
     6121 ttggttcatc tcagagctta caggacctga aacaatcaat ggcgtcctag cttcatcgat
     6181 taatacagag tcaacctcat caaccaaagc atagttgagt gggcgttgca ccatatcttc
     6241 ttggcgaaca accatattgt ctcgaaggta gtcaaatccg acttctgcgt tagttgagta
     6301 tgtgatatca caattgtaag cttcgcgttt ttcaaatggt gacttagctg ctaagttaat
     6361 ccctacagat aatccaagcc aactataaac ctcacccatt tcggtcgcat cacgtgttga
     6421 taagtactca ttaacagtaa taacatgaac acctaatcct gagatagcat ttagataaac
     6481 aggcattgtc gctgttaaag tttttccctc accagtacgc atttctggaa tatcaccatg
     6541 gtgaagaacg ataccaccca taatttggac gtggtatggg tataaaccta atacacgttt
     6601 agaagcttca cgaactaccg caaacgcttc cggtaacaat tgatctaaag tttcaccatt
     6661 ttgataacgt tctttaaatt ctggagtttt tgcttgtaaa gcttcatctg ataacgtagc
     6721 catatgatct gcgtaagagt caactttctt agctatttta tctaattttt ttaattcacc
     6781 cttgtcattt tcaataacag tgcgtagaat atttgccatt ctaattcctt tcgatactct
     6841 tatcaccaca ttttaccata aataatgttg tctttcaata aatggtcgtt ttaatcgaga
     6901 ttaaaacaag tggtatcccc actctcatta ttttttaaca tgacttgcta taatttctaa
     6961 ctgtccatca atatcccatt tgacaacatc atttggcaag ataaagtggt cacctttttt
     7021 aagtgtataa actttgttat ctactgtaat gtgacccgct ccgtctaaga cactaaccaa
     7081 aaggtagggt gcaaattgtt taaagttggt aacaccagaa atttcccatt tataaactgt
     7141 aaagaaatca tttgaaacta ataaagttga agatagatgt tttaacttca tattggcagg
     7201 aacagtattg gcaggtttac caatcgtgag aacatctatt gattgttcaa tgcgtaaatc
     7261 acgtaatttt cctgaagcat ctggacgatc aaaatcataa acacgataag tagtatcact
     7321 agattgctgt gtttctaaaa ttaatatccc tttacctata gcatgcattg tcccacttgg
     7381 aacgtagtaa aaatcccctg atttaacagg aattcgggtt aaaagatgtt cccaatctcc
     7441 agattcaatc atttggcgaa gttcttcttt agttttagca ttatgaccat agataatttc
     7501 agatccttca tcagcagaaa taatatacca acattctgtt ttaccaagtt caccttcatg
     7561 tttaagagca taagcatcat ccggatgaac ctgtacacta agccagtcat tagcatctaa
     7621 gattttagtt agtaaaggaa agacagtatc atcagggttg ccaaataagc ttttttcacc
     7681 ttgatacagc ttatctaaaa aacaaccttt aaagcgacca ttcttaactc ttgagacacc
     7741 gttaggatga gctgaaatag cccaatattc tcctgttgtt tcacttggaa tatcatagcc
     7801 aaattcatcc cttaactttg ttccacccca gattttatcg tgcatgcttg cttctaaaaa
     7861 taatggttcc gacataagaa tcccctatct tttgtaaaat tcaagcctat tatagcattt
     7921 tatccatcat atcgcttact ttttttaatc ttttatttgc taactaaact aagtagtggc
     7981 ttaagggttt tattaacctt ttgatatttt ctttgctaaa gcaaaatttc ctaacgttgc
     8041 agaaccattt tcttctatag ctggtgtcac aatataatca gacaaatcgg gaactggtaa
     8101 atacccattt aataaagttg caaatgtctg gcgaacacgt cttaacatgt gctcttgtgc
     8161 catgacgcct ccaccaaata caatgacttg tggacgatat aatacagttg cttgtattgc
     8221 agcctgagca atataatagg cttgaacatc ccaaaccatt gaattttctt caatcaattc
     8281 accacgaata ccagtcctag cttctagagt tggaccagat gccaaacctt ctaaacagct
     8341 attatgaaac ggacagatac ctttaaaatc atttgcttgg tcctgcggat gcatcgccat
     8401 ataagtatga cctgcttcag tatgaccagt tcctcctata aattcaccct tttgtatagc
     8461 tcctgcacca attcccgtac caatggtgta ataaacaagt gaatcaatat tgttacgagc
     8521 tataacttca ccataggcag agctattaac atctgttgtg aaataaaatg gaacattgaa
     8581 atctttagaa attaagccga gtaaatcaac attagcccaa tgtaactttg gggtcgttgt
     8641 gatataaccg tatgtctttg attttttatc aatatcaatg ggaccaaaag aaccaatagc
     8701 aactgcctct aacttctttt caaatctttt aaagaaatca acagtctttt taatcgtttc
     8761 ttgaggcgtc gttgttggaa attgcatttt ttctacaact tttaattcct catcaccaac
     8821 tgcacaaaca aactttgtcc cacctgcttc aatactacca tataactttg tcatcttttt
     8881 tcctatctat aatctctatc tcaaagacaa aggttgggat aattatccca acctttaatc
     8941 ctcacttaac atcaataatc atgtcacctg tagtaacaac accctctttt accaatggta
     9001 taacatcagc aaaatcagct gtattagtca caataatcat agctgtatta tccagacctg
     9061 cttctgcaat cttggctgaa tcaaaggtgc caagaatatc accttttttc actttttggt
     9121 ctgccttgac tttagaagta aaccctgtgc cattcatggt gacagtatca atacctatat
     9181 gaatcaaaat ttctgcacct ttatctgatt tgataccgta agcatgacct gtttcaaaag
     9241 caatttggac aaaaccatca acaggtgagt aaattgtatt acctcttggt ttaatagcaa
     9301 ggccatctcc cataatacct gatgaaaaaa ctgggtcact tactgtatca agagcaataa
     9361 cctgtccatc aagagggcta acaatcgttt ctgcccttaa ttggctagtt tgagtaattg
     9421 ttggatcgtc atctgtttct tcaaccaaag gactaacttc agctactgct ttttcatctt
     9481 tatcttcata tccaaacata taagttaata caaaagccaa agacgtagtt ccaattacca
     9541 taataaggta cttaacaatt tgaccattta aataaagtaa tgttcctggg ataatggtaa
     9601 taccaaaacc agttcctgct aatttcaata ttgaagctaa ccagccacct gctgctcctg
     9661 caattaatcc cataataaat ggcttaccaa aacgtaagtt tactccaaat atagccggtt
     9721 ctgtaatacc aagtccagct gaaagcgctg ccggaaaagc aagggctttt aattttttat
     9781 tttttgtttt aacaccaact gccaacgtgg cacctgcttg tgctgtcata gcagctgtaa
     9841 taatagcatt aaatggatct ttcccatcag cagcaattaa ctgagcttca agtaaattaa
     9901 agatatgatg aacacctgta acaacgatta tctgatgaac accacctaaa ataagacctg
     9961 ataggccaag aggtaaatta aggacaaatt ttgtccctgc taaaacatag ttttcaactg
    10021 aatggaaaat aggaccaatg acaaaaagtg ctaaaattga cataactgta aaagttagga
    10081 acggcactaa tagtaaatca agaacatctg ggattttctt atgcaaccat ttttcaagtt
    10141 tagcaccaag taaacctaca aagaatgccg gcaatactga attttgataa ccaactactg
    10201 gaataaagcc gaaaaatttt atgggatggg catcacctga ggccactgcc caagcgtttg
    10261 gaagggcaga gttgaccatc atcaatccta atacgagacc gataacagga tttccaccaa
    10321 aaactctaaa agctgaccaa gaaatcaagg caggaaaaaa agcaaaggcc gtgtcagtca
    10381 aaacgacagt ataggtataa aaattagaac ttgaaaatgc ttttgaagtc gtcccaaata
    10441 aagctaaaac tgtatcatta ttaatcgcac cgcgaatccc cataaataaa ccagtagcaa
    10501 caatggctgg caacaatgga acaaaaacat caccgaaagt acgaatagct cgttggaaag
    10561 cattcccttg tttagccgct tctgcctttt gatcagaggt tgacaaagtt ggcaaacctt
    10621 gagccactac ttcatcataa atcttattaa cagtaccagt tccaaaaata atttgatatt
    10681 gacctgaatt gaaaaatgct ccctgaactt tttcgatatt ttcaacagta tttttatcaa
    10741 tgactgtctc atcttttacc ataatgcgaa gacgagtggc acagtgtgct acgctacgca
    10801 cattactagc accaccaatc gcattaatga cttgtttagc aatttctgta tttgaagcca
    10861 tctataaatc tcctttgaca ttttgaaagc gtttcatttt atttactcta atattctagc
    10921 atgtctcata aatatatgtc aagcgtttga cataaaataa tattttttga tgttaagagt
    10981 tgatttcaaa agagaaaacg tttactatat agttattaga tattaagaca gtcaaggagt
    11041 atcaaatgaa tttacccact gaaatccgct atcgtcctta tgatgaatgg actgaagaag
    11101 ataaggaaaa tattttaaag aatgtctcaa aatccccttg gagagcaact tatcacttag
    11161 aagctaaaac agggctttta aatgacccaa atggcttctc ttactttaat ggaaaattcc
    11221 atctttttta tcaaaattgg ccctttggag ctgcacatgg tttaaaacaa tgggtacata
    11281 cagaatcaga tgatttagtt catttcaaag aaacaggaat caagcttaaa cctgaccatg
    11341 tcaatgatag ccatggtgca tactccggtt ctgcattagc cattgacgat aagctttttc
    11401 ttttttatac tggtaatgtc cgtgacatga aatggaaccg tgacccccgc caaattggag
    11461 catggatgac aagtgatggc aagattacaa aatttgataa agtccttatt tcacaaccaa
    11521 atgatgtcac tgagcatttt cgtgatcctc agattttcaa ttatgataat caattctatg
    11581 ctgttatcgg ggcgcaaaat agtaaaaaat gcggattcat taaattatat aaagcactta
    11641 ataatgacat ccatcattgg gagtttgtgg gagatttaga ttttggtggt actggatctg
    11701 agtatatgat tgaatgtcca aatattattt ttgtcaaagg aaaacctgta ttactttata
    11761 gtcctcaagg actagataaa aacgaattgg actaccaaaa catttatcca aatacctaca
    11821 aaatcggaca atatttcgat gctaactcat ctaagatagt tgaaccatcc cctatctata
    11881 atttagatta tggttttgaa gcctatgcta ctcaaggatt taatacttct gatggtcgta
    11941 cctttatcgt gagttggata ggcttgccag atattgatta tcctagcgat caatttgatt
    12001 atcaaggagc tatgagttta gttaaagagt taagcataaa aaatgggaac ctttatcaat
    12061 atcccgttcc tgctatgaaa aatcttcgtc aacatcaagc agaatttaaa acacaacttc
    12121 aaactaacaa cacctatgaa ttagaattac tagttcctag aaacgatctt tcttcatttg
    12181 ttctctttgc taatccgaaa ggacaaggac tatccattac tattgatact gcaaagggga
    12241 aagttatcgt tgaccgctca caagccggac agcagtacgc cacagaattt ggtactagtc
    12301 gccagtgtga cattccaaaa gatgctacta gtatcaatat ttttatcgat aagtccatct
    12361 ttgaaatctt tataaataaa ggagaaaaag tatttacggg acgagtcttt ccagatgcag
    12421 aacaatcagg aattcaacta aaagaaggac acgttcatgg taaatatttt gaattaaaat
    12481 attaatatgg ttgcaaaatt aacagatgtt gctgcacttg ccggtgttag cccaacgact
    12541 gtatcacgcg ttattaacaa aaaaggctat ttatctcaaa aaacagtcac caaagtcaat
    12601 gaagccatga gaacccttgg ttataaacct aataatttag cacgtagctt acagggaaag
    12661 tctgctaaat taattggact cattttcccc aacatccgta acatttttta cgctgaatta
    12721 attgaacacc ttgaaattga gttgtttaag catggttata aaactatctt atgtaatagt
    12781 gaaaaggatc ctatcaaaga aaaagaatac ctcgaaatgc taggagcaaa tcaagtagac
    12841 ggtatcattt caagtagtca caacctagga attgatgatt acgagaaagt tgaggctccc
    12901 atagtagcat tcgatagaaa tttagctcca cacatcccga tcatctcatc tgataatttc
    12961 tttggaggaa aaatggctgc tcaaaccctt aaaaaacatg gctgtcaaaa gatgattatg
    13021 attacaggga atgataattc agattctcct actggactta ggcggctagg tttttcctat
    13081 gaatcaaagg aatccaaagt tatcacagtc acaaatgggc tatctaacat gagacgtgag
    13141 atggaattaa aatcaattat ctcgactcac aaaccagatg gtatttttac atcagacgac
    13201 ttaactgctt tattagtaat caaacttatc agccaactag ggttgtccat cccagaagat
    13261 atcaaagtga ttggatatga tggtacaagt ttcattcaag attacgtccc acatctaacc
    13321 actattaaac aacctattcg agaaattgcg caattgatgg ttgaaatact cctagctaag
    13381 attgaggggc aaaagacaaa taaggattat attttaccag tcagtctcat ccctggtagt
    13441 agtgtttagg aaatcatatt tataaaataa tttttagtaa tagttatttt tcaaagcaaa
    13501 ttaaaaatgt ggaagaatct tcttccacat ttttaatttt catttgttat aaattggctt
    13561 aataaaccat tgacaaattt tgctgacgtc tcatctgaat attttttagc aatctcgata
    13621 atctcattta gagctacacg atcaggcgtt tcatcaaaat atttaatttc atagaggcct
    13681 aagcgtaaga gagatttatc aaccaatgtt agacgttcta agctccatcc tgattttaag
    13741 tgactagaaa tcaaagtatc taattcatcc ttgtgatcta caacaccatt aacaagatta
    13801 agtaaaaaga ttggaacctc taaaactttg tcttcagata ctgttttatc ataaccataa
    13861 gcaaagtgag ccgcatcaat aaactctcca ccagtttcta agctaaatag tgtttgaaaa
    13921 gcacgttcac gtaaatcacg tcttgaatct ttaaatacac tagtcatcta agaaatcctc
    13981 atcaaacaat gacttcaagt ctggttttgg tgttttctct ggaacaatac cttcaacatg
    14041 gatattgacc gaagaaattt ttacttctgc catgtcatat acagctgttt tgacagcttg
    14101 ttgaatagca attgaaactg ctggtacatt tacaccatac tgcaagtaaa cataaatatc
    14161 tgcagctact gtatcatctt cttcattttt aaggtagaca cctctaccta gagatttctt
    14221 actaaggcta tccgttaccg ctttgttacg taacgaatga acaccatcaa ctttagttgc
    14281 agcaatacct gtaattacct caagtacacg tggtgaaatg acaatttctc caagattttc
    14341 agttgtcata gcttatcctt tcttttagtg tttactaagg tttttcctta gtagagtaaa
    14401 ttcatcatga atctacttaa cacttttttg agagtcatta ggcgcgtgaa acgtaagttc
    14461 cttctgctgt attgattact aacttttgtc cagcttcaat aaagtctgga acgttaacaa
    14521 caagtcctgt ttccatagtt gcaggttttc ctgaaccagt tacagtagca cctttgattg
    14581 atggttgagt ttcagcaact gtcaattcaa cagttgttgg aatttgaaca ccaataactt
    14641 ctgttccata gaattgaatt tttacttctg agttttcaag aatgtagagt aattcatttt
    14701 caatgttaac agttggaatt tcatattgat cgtaagtttc attattcatg aagtaagctg
    14761 tatcatccat cttataaagg tattgagcag gtacagtttc aataatagct tgctcaaatt
    14821 tttcttcagg acggtaactt gtatcaaaag ttgaaccagt acgaacatca cgcaatttca
    14881 tacgcatgat tgtgttacct ttacctggtt tgtggtggct agcttcaaga acacggatta
    14941 atttcccatc agccgtttca aaagtcatgc ctgctttaag cttacttgct tcaatcattt
    15001 attaatacct ctttgtaaaa attatttatc aactaatttt atcacaaata acctgctttc
    15061 tcaaatagtt tattgatttg gtatgtgtct gatttcttga taaataaata aaatcaccaa
    15121 gagggcgtaa ataagactga agcacttgac actttcctct tacttcagtt ttgataacag
    15181 ttttaatatt ataccgatat cttgactaag attgaaatac tgtttaggtg tttttagtga
    15241 ctttttagta aattttaata aaggtagtaa actatagttg tcttattgaa acagataaaa
    15301 taaatcacct acggagctat tcgtatgaaa atgtagttat tctcgatata agttttagat
    15361 acgaacttaa tttgctctat tgaagctggt aaaggaaatc ggggtggtgt tagtggacgt
    15421 gcgctaagca ttggtactga gcttggtgat accaatcttg cttactacat caggtagctc
    15481 tctgttttag tgccatatgc aattggaata agagctgtca ttggtattga aggagcccta
    15541 gtccactgat cattgaatta ctaatttagg aggtggaatg atgaatttta atatgtctca
    15601 ttatagaaag ttacaactat gtcgttttct aatcgtacag atacctgcgc tggctttatt
    15661 agctattctt ttatacggga ttttcctaaa gtacagtcta ttttcaaaag tattttttct
    15721 tatcttttag gggttgaaat agttatcaat tttgggaagt acataccagt ttacaccaac
    15781 cacaatttgt cgagtaaaga attgtaattt agtaaagcct ttttcgtttt cttctgataa
    15841 gtgtatcatg acatctatca atagcatatt cacactagta gcatattgat ttgtttttga
    15901 gtagaattta ctttttagat tatttctatt ctacactagt ctttcccttt ggaagattct
    15961 ttccctctgt atttttccat ttttcaatga gacgagaaac tctttcttcc tcctcatcat
    16021 ggtaaccttc aaaaacaacc tcatcaatat cttcttgatt aaaatagtaa acttgttcaa
    16081 gatttaaacc ctcaggaaaa attccaccta ggtaatcaaa ataaacttct tgtccttctt
    16141 gctcaacaat tgcacctcta tttacaataa ctaatttttg attgccatca ataagataga
    16201 caacactccc tactggtaat aacatacttc agttctcccc aatataataa tttttcccat
    16261 ttaacgatgg ttgaaactca atgaacttta taaaaaataa actgtttgaa aaaataaatc
    16321 caaataaaaa taagtttgaa aagt
//
LOCUS       DATROY010000035        16333 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.035, whole genome shotgun sequence.
ACCESSION   DATROY010000035 DATROY010000000
VERSION     DATROY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(138..1421)
                     /locus_tag="VBD11_000907"
     CDS             complement(138..1421)
                     /locus_tag="VBD11_000907"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6953871.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            complement(1423..2700)
                     /locus_tag="VBD11_000908"
     CDS             complement(1423..2700)
                     /locus_tag="VBD11_000908"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6953872.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            2749..3588
                     /locus_tag="VBD11_000909"
     CDS             2749..3588
                     /locus_tag="VBD11_000909"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6953873.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            3668..4162
                     /locus_tag="VBD11_000910"
     CDS             3668..4162
                     /locus_tag="VBD11_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6953874.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            4380..5606
                     /gene="clpX"
                     /locus_tag="VBD11_000911"
     CDS             4380..5606
                     /gene="clpX"
                     /locus_tag="VBD11_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6953875.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            5617..6213
                     /locus_tag="VBD11_000912"
     CDS             5617..6213
                     /locus_tag="VBD11_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6953876.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(6275..6823)
                     /locus_tag="VBD11_000913"
     CDS             complement(6275..6823)
                     /locus_tag="VBD11_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6953877.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            6915..7564
                     /locus_tag="VBD11_000914"
                     /pseudo
     CDS             6915..7564
                     /locus_tag="VBD11_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            7576..8226
                     /locus_tag="VBD11_000915"
     CDS             7576..8226
                     /locus_tag="VBD11_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6953878.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            8356..9732
                     /locus_tag="VBD11_000916"
     CDS             8356..9732
                     /locus_tag="VBD11_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6953879.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            9737..10681
                     /gene="mmuM"
                     /locus_tag="VBD11_000917"
     CDS             9737..10681
                     /gene="mmuM"
                     /locus_tag="VBD11_000917"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6953880.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(10774..12882)
                     /locus_tag="VBD11_000918"
     CDS             complement(10774..12882)
                     /locus_tag="VBD11_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6953881.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAEQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLM
                     LDEVNQTIGKKGIDLVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     misc_feature    13152..13285
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            13315..13815
                     /locus_tag="VBD11_000919"
     CDS             13315..13815
                     /locus_tag="VBD11_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6953882.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     gene            13879..14244
                     /gene="rplL"
                     /locus_tag="VBD11_000920"
     CDS             13879..14244
                     /gene="rplL"
                     /locus_tag="VBD11_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6953883.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            14539..15540
                     /locus_tag="VBD11_000921"
     CDS             14539..15540
                     /locus_tag="VBD11_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEO6953884.1"
                     /translation="MKSIELFAGAGGLALGIEKAGFDTIGLVEFDSAASETLKYNRPN
                     WNVIHDDVANISKLDLEEYFLIKKGELDLLSGGAPCQSFSYAGKRLGLEDTRGTLFYH
                     YAVFLEKLQPKMFLFENVKGLTSHDKGKTYKTILNVFEEEGYTVQSKVLNAWDYGVAQ
                     KRERMIMVGIRNDLVNMTSFEYPKPHDYKPVLRDILLDCPPSQGMQYSEQKRKIFELV
                     PPGGYWKDIPESIAKEYMKSTWYMGGGRTGILRRLSLDEPSLTVLTSPTQKQTERCHP
                     LEVRPFTIRENARIQSFPDDWEFQGSVGNQYKQVGNAVPVNLAYEVGLKIKESLEVL"
     gene            15541..>16333
                     /locus_tag="VBD11_000922"
     CDS             15541..>16333
                     /locus_tag="VBD11_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="HEO6953885.1"
                     /translation="MWGLSFISEEDFYNHVQLTIDKYGKKLQSFDLKRFNKNIVDPIK
                     LIFDKSVYRSSWEQIISNEIFRQRDKSNNNDIGYFHQTIFQYIEHCRVPNNGEEGGWD
                     VIFENPNGITMPDGSQVSRVYVEMKNKHNTMNSASSGKTFMKMQNQLLQDDDCACFLV
                     EAIAKNSQNIQWEPKVDGRKMGHKYIRRVSLDQFYSLVTGQEDAFYNMCMVLPEVINK
                     AVSELDSSIIPNDTVFDEIRDISAQQNYDSEDLSIAMSFYLLGFSS"
BASE COUNT         4905 a         2789 c         3234 g         5405 t
ORIGIN      
        1 ctagttcagg taattcttcc cattttgggt attgattttt attcatttta aaactccata
       61 taatctagtc tttataacta gattatacac ttatggaaat tcaagtcaag taaaaaaaga
      121 atttgagatg atttttctca aattcttttt ttacttttag taagtttatt gacaattttt
      181 tgtgctgtct ctagattcat gtgtttagag tctagaagct gtttaacgac ttctgaaatt
      241 tgttcttctt tagctccagc aagtagtgcc aacgactttg cttgaagttt catgtgacca
      301 gcttgaattc ctgtgctaac caatgcctta agagccgcaa aattttgagc tagtccaatc
      361 gaaagaataa tacttgccaa ctctttagca ttaggacgac ctagtagatc gtgagctagg
      421 tggacactag ggttaatacc aatggatcca ccttttgttg caacaggcat cggtagcgtc
      481 agagtacctt ctaggcaatt atcatcaacc ttataagacc atctagataa tcctcgatat
      541 tgcccatctt tgacagcata ggtatgcgct ccagcttcaa tagctcgcca atcatttcca
      601 gtagctaaga cgatagcatc aatgccatta aaaatacctt tattatgtgt gctagcacga
      661 taagggtcta cttgagctaa ttgacttgcc atcgtcattt tttgagctaa cttgatggcc
      721 tcctcttttt ggcgacttag aaatcttaaa tcaactcggc atgtagcagt cactaaagat
      781 tcggtagcat agttagataa aattgacatt aagcttttgc cttttgaaat gtcttctaat
      841 ggcgaagata atgcttccat catggtgtta accatattag cacccatggc ttcttttgta
      901 tcaaccataa ggtaaacaat caaaaagtct tctttaatct caatagttag gtcgcaagca
      961 ccgcctccac gcttgactat tgatggatga gcagcgttgg ctatttctaa taattgctgt
     1021 ttttgattta atatagattc tttagtgtgt tgactatctt gtacatcata aagggctact
     1081 tgtccaatca ttttacgatt atggacggtt gtcagaaaac caccagatcg ttttataatt
     1141 ttagcagcaa aacttgcagc agcaacaact gacggttctt cagtgacata tgggacttga
     1201 tagactttac cgttaaccaa aacatctggg acaagtgaaa atggtaaggc tattcgaccg
     1261 atgacatttt cagccatttg attggctagt gataaagaga gtaagtcctg attcgtcact
     1321 atttctaggg aagaatcagc taaaaaatct tgttcttcaa ggtaatgaat acgttcttca
     1381 ggggattttt ttgaaaatcc tgtccaggaa atttttgtca tattaattat taattttctt
     1441 gtagatacgt tgatgttcgc aaatagagtc tagagaaaaa gtgccagtcc ggtaggtgtt
     1501 gaagttagca tttccatttt cgtctaggat tgttttttca aagaagagtt tctcatactc
     1561 agttactgta attttttgac gtttattgag ttgatccata cgatcgctct gaagtttatt
     1621 ttggtagccg tctactaact gaccggtaaa aatttcagca acagctccgc taccgtatga
     1681 aaagaaggca atttgatcac ctgcttttaa attttgacta ttttctagta acgatagaag
     1741 tcctagatac aatgagccag tatagcaatt tccaattttc ttactatagg taatagagtg
     1801 ctcaaaattt tcttgtagtt cagctttctt ttgttcatct aagttattat ctagaatttt
     1861 attaaagccc tttaaagcta attttggaaa aggaatatgg aaacaaaaag cagcatagtc
     1921 acttagacta gtattaaaac gtttttgata tactttccaa gtagttttta gcatatctag
     1981 atattgtttt gttgaaaaca tgccattaac ataaggaaca tcagaataat ttggtcgcca
     2041 aaagtccata atatcacggg tttgcgctag atggtcctct tttagctcta gaatggatgg
     2101 attttgacta ataagcatag cgatactgcc tgcaccttga gtggattctc cagtagattt
     2161 aatgccatat ttagcaatat ctgaagctat cactaatact ttagaatctg gatgtttttc
     2221 tacatgtaat ttagcgtaat caagagcagc agttgcgcta tagcatgctt ctttcatctc
     2281 gaatgacctt gcgaaaggtt gaatctctaa aagttgatgt acatagacag aagccgcctt
     2341 actctgatcg atacttgatt ctgtagcgac aatcaccata tcaatagttt ctttgtcctc
     2401 atcagataaa atatcattag ctgcagaagc tgcaagtgta acgatatctt ctgtaactgg
     2461 cgtaatacta agtgaatcta acaataatcc cttgctgaat ttttcagggt caacttggcg
     2521 agcaatagcc aaatcagtca tttcaaggac atattgactg gttgcaaaac caatcttatc
     2581 aattcctatt ttcattggta aacctttctg aataaaagtc atttttgcta aattccattt
     2641 taccataaag aagctaaaaa tatgaaaaaa agctttaacc ttcaaagtct tgtttctcac
     2701 tagaatatct ttttaaaatc tgataaaata agactttgga ggtatctcat gacaaaagcc
     2761 gatttattat ttaaagacaa tatcacgaaa attatgtctg aaggtgtatt tagcgagcaa
     2821 gccagaccta gatataaaaa tggggaaatg gctaattcta agtatattac aggtgccttt
     2881 gcagagtatg atttatcaaa gggtgaattt cctattacaa ctctacgtcc aattccaata
     2941 aaatctgcta ttaaagaaat tttctggatt tatcaagatc agacaaatga tttagcagtt
     3001 ttgaatgata aatatggtgt gacatactgg aatgattggg aagttggcca tactggcacg
     3061 ataggtcagc gctacggtgc agttgttaaa aaacataata ttatatcaaa actcttaaaa
     3121 cagcttgaag ataacccttg gaatcgtcgt aatgttattt cattatggga ctatgaagca
     3181 tttgaagaaa cagaaggcct actaccttgt gcttttcaaa cgatgtttga tgtgagacgc
     3241 gttaatggtg agctttattt ggatgccaca ttaacacaac gttctaatga catgttagtt
     3301 gcacatcata ttaatgctat gcagtatgta gcacttcaga tgatgattgc aaagcatttt
     3361 ggttggagag ttgggaagtt tttctatttt atcaataatc ttcatattta tgataaccag
     3421 tttgaacaag ctcaggaact cttaaaacgc caaccgtctg agtgtaaccc caaactagta
     3481 ttgaatgttc cagatggaac agactttttt gatattaaac cagatgattt tgcgttagtt
     3541 gattatgacc caattaaacc tcaattacgt tttgatttag caatctaact atctcatgtt
     3601 ttttaacaag caatttgagg taaaatttgt tagaatatct agagataagt tagtgaggaa
     3661 aaaagtcatg acaaagcaaa taattgccat ttgggctgaa gatgaagacc atttgattgg
     3721 agttaatggc ggtttaccat ggaggcttcc taaagagtta catcacttca aagaaacgac
     3781 catggggcag gctttgctta tgggacgaaa gacctttgat ggaatgaacc gtcgtgtttt
     3841 acctggtaga gagacaatca tcttaacaaa agatgaacaa ttccaagcag atggagtgac
     3901 agtcctaaat agtgttgaac aagttataaa atggtttcag gaacataata agaccttatt
     3961 tattgtaggt ggtgcaagta tttataaagc atttctgcct tattgtgaag caatcataaa
     4021 aactaaagtt catggaaaat tcaaaggtga tacctatttt cctgatgtta atctatctga
     4081 gtttaaagtt atctctagag attattttga aaaggatgag cagaatgctc atgcttttac
     4141 tgtgacatat tttgaaaaat aaggaaggaa tactattcaa tgcaacgtag cctttttggg
     4201 gtctttaccg catttttagc tgtgatttgt gttttatgtg caattcctgc tataaagaaa
     4261 aaaagatatg gattaggaac agtttttttg atgaatgctt tcactaacct tgtcaacaca
     4321 atccatgcct tttatggaac attattttaa attaaatgaa tagaaaagtt gaggaaaaaa
     4381 tggctgggaa tcgtaataac gatatgaatg tctattgttc attttgtggc aaaagccaag
     4441 atgaagtaaa aaaaattatt gcaggtaatg gtgttttcat ttgtaatgaa tgtgtggcct
     4501 tatcacaaga aattattaag gaagaattag ctgaggaagt actggctgat ttagcagaag
     4561 taccaaaacc taaggaacta ttagaaatat taaatcaata tgttgtaggg caagatcgtg
     4621 ctaaacgtgc tttagcagtt gctgtctaca atcattacaa gcgtgttagt tataccgaga
     4681 gtagtgacga tgatgtagat ttgcaaaaat ccaacatttt gatgattggt ccaactggct
     4741 caggaaaaac cttcttagca caaacactgg ctaaaagcct taatgtaccg tttgctattg
     4801 cagatgcgac ttcattgacc gaagcaggat acgttggaga agatgttgag aatattcttc
     4861 ttaaattgat tcaagctgct gattataatg tcgaacgtgc tgagcgtggt attatctacg
     4921 ttgatgaaat agataaaatt gctaagaaag gcgaaaatgt ttctatcaca cgtgatgtgt
     4981 ctggtgaagg tgtacagcaa gcccttctta aaattattga gggtacggta gcaagtgttc
     5041 ccccacaggg tgggcgtaaa catcctaacc aagaaatgat tcaaattaat accaagaaca
     5101 tcctttttat tgtcggtggt gcttttgatg gtattgaaga ccttgtgaag caacgtttag
     5161 gcgaaaaagt tattggtttt ggacagacaa gccgtaaaat tgatgacaac gcttcttata
     5221 tgcaagagat aatttctgag gatattcaaa agtttggact gattccagag tttattggcc
     5281 gtttaccagt agttgcagcg ttagaacttc ttactgcaga agatctggtt cgtattctga
     5341 cagaaccacg caatgctttg gttaaacaat accaaacctt attatcttat gatggtgtag
     5401 aattggaatt tgaccaggat gctctattgg ctatcgctga taaggctatc gagcgcaaga
     5461 ctggtgcacg tggtttacgt tctattattg aagaaacgat gcttgatatc atgtttgaaa
     5521 ttccaagcca agaagatgta acaaaagttc gtatcacaaa ggctgctgtt gagggtactg
     5581 acaagcctgt tttagagacg gcttagggag atagagatgg cagaagaatt tttaaatact
     5641 cataatgcgt ctattttatt gagtgcagct aacaagtcac attatcctca ggatgatctc
     5701 ccagaagttg ctctcgctgg tcgttcaaat gttggtaaat caagctttat caacacgctt
     5761 cttggtcgta aaaacctagc gcgtacgtcg agtaagccag gtaagacaca actgctcaac
     5821 ttttataaca ttgatgacaa actgcgtttt gtcgatgtcc ctggatatgg ctacgctaag
     5881 gtgtctaaaa ctgagcgtgc taagtggggc aagatgattg aagagtacct tgtaacacgt
     5941 gataaccttc gtgttgttgt tagtttagtt gattttcgtc atgatccaag tgcggacgat
     6001 attcaaatgt atgaatttct taagtactat gagattccag taattattgt agcgacaaag
     6061 gcagataaaa tacctcgtgg caagtggaat aaacatgagt cgtctattaa gaagaaatta
     6121 aactttgata aaaaggacca tttcatcgtc ttttcatctg ttgaccgcac aggtcttgat
     6181 gaatcatggg atactatttt atctgagtta taatttgaga gtttatgaca aaaagagctt
     6241 tgcagtagct gttgagacta ctgcaaagct ctttttaatt ttttaaatga ttgataatat
     6301 cgaaggaaac ttgagttaat tcttcgatac ttgtttggca gtcctgatga atccactcaa
     6361 ttagaacact gaaaatacca ccaatcgcaa atcgctgagc ataggtattg gaagataaat
     6421 caccacgacc taactctatc aaatcaatat gtcggaacca ggattgttga aattgaggga
     6481 gtaggtcatt ctttctaaga atgagaagaa gggatttata atcagaccaa taactaaaaa
     6541 aattttggaa aacttggcta aaagtaggtt gctccaattg accaagttgc ttaaagagat
     6601 ttaatgttag tctttcgatg aaaagggtaa gtaggtcatc tcgactgtca aagtgtcggt
     6661 aaaaggtcct tctagcatag ccagatttct tggcaatttg gctaatctga attttctcat
     6721 agggatactc ttccaaaagt tggccaaaag ctgttaagat atcctcttgt gtttgagtga
     6781 tttgttttat tctcttttta ttaactgtca cactttcagt catttgtgca taaaacctcc
     6841 aaaggtattg atattatagg ttcacttgat tatactatat ggtatcaaat gtcacaaatc
     6901 ttggaggtca tcttatgaaa gcattgataa caaaatggta cctcttttgt ccctatcttg
     6961 cttctctttt tgccttagca ttattttttg gaaattgaga tttacgagtg cagtcactac
     7021 ttatttcggg actttttatt caattacact ttttcgaaga atttggtttc cctggaggtt
     7081 ttccgcttat cactatgcta gttgaactta aatcggttga aactgatact agtaagtggg
     7141 acttaaacca tctatcagcc tttttcggga atcagtggtt tgcagtgatt gtttatcttt
     7201 tgccgatttt ttgtcccaat ataccatttt taacacttgc agtcatgatt tttgcatttg
     7261 cagaacttgc catgcatctt ttctttttta atctttcctt gaagaagtgg tataatccag
     7321 gacttcttac gaccttagta ggtcttgtcc ctgtttctgt ctattattta gctcatgatt
     7381 ggaagttgta ttctggactt gattggtttt taggctgata tggattgttc ttaactactt
     7441 tatagccttt cgttccccaa tctataagcg tttaggtcga cattcaaatt acgcatttag
     7501 tgatgttgat ctttcacgct caaaaccatt tttaacacat tttcgtgaaa cacagtttaa
     7561 ataggaggta ttatcatgtc atattttaga aattactggt atcgttttgg agcaatttta
     7621 tttattattt tagcagtaat attgcttgtt tttagacctg actggtcaat gcttcactat
     7681 ctattgtatt tttactttat ggcacttcta gcgcatcaat ttgaagaata tcagtttccc
     7741 ggtggggcat cacctatcat taactatgtt gtttatgatg aagaagagct gatggattgt
     7801 tttccaggca atactcagtc tattatgttg gttaatacta ttgcttggtt gctttacatt
     7861 gctagtattg cttttcctca agcttattgg cttggattag gagtcatgtt ctttagtcta
     7921 acgcagctct tgggtcatgg ttttcagatg aatattaaac ttaaaacttg gtataatcct
     7981 ggtctagcaa cgacagtatt tctcctagta ccaatagctt gcgcatacat ctatcaagct
     8041 agtgcagaag gaatgctcac ttggggagat tggctaggtg gttttatcat gttgattgtc
     8101 tgtgtactaa ctagcattat tgcacctgta cagctattga aggataagga gaccaattat
     8161 attattagtc cttggcaaat ggaccgtttt cataaggtcg ttaattttgt aaggataaaa
     8221 aaataaggtt accgctattt atgattcgct gattttttgt aatagttttt ctttatcata
     8281 tatgatagct aatacctatt ccccaatacg actagattgt ggtaggataa aagtattagt
     8341 ttcttggagg ttgttatgga aaatcatcgt tatgaagatg aaggtaaatt ccagcgtaag
     8401 atgaccagtc gtcatctctt tatgttatcg ctaggtggtg ttatcgggac tgggcttttc
     8461 ttgagttcag gttataccat tgcacaggct ggtccgcttg gagctgtgct gtcttatttg
     8521 attggtgccg ttgtggttta tttggtcatg ctatcacttg gggaattggc ggttgccatg
     8581 ccggtgacgg ggtcattcca cacttatgcc actaagttta tcagtcctgg aacaggtttt
     8641 actgttgctt ggctatattg gatttgttgg acggtcgcct tggggactga atttttaggt
     8701 gctgccatgc tgatgcagcg ctggttccca aatgtgccgg cttgggcatt tgcttccttt
     8761 tttgcccttg tgatttttgg tttaaatgct cttagcgtac gcttttttgc agaagcagag
     8821 tctttcttct caagtattaa ggttattgct atcattatct ttattatctt gggcttaggt
     8881 gctatgtttg gtctagtttc ctttgaaggt cagcacaagg ctattctctt cactcatctg
     8941 actgccaatg gtgcctttcc aaatggtatc gttgcagttg tctcagtcat gttggctgtt
     9001 aactatgcct tctctggtac tgagttaatt ggtattgcgg ctggtgaaac ggataatccc
     9061 aaagaagctg taccaagggc tattaaaacg acaatcggtc gcttggttgt tttctttgta
     9121 ctgacaattg ttgtcctagc ttcgctattg ccaatgaaag aggcaggcgt atccacagca
     9181 ccattcgttg atgtctttga caagatggga atccctttta cggcggatat catgaacttc
     9241 gttatcttga cagccatcct gtctgctggt aactcaggtc tctacgcatc aagccgtatg
     9301 ctctggtccc ttgccaatga aggtatgttg tcaaaatctg ttgtgaaaat caataaacac
     9361 ggtgtcccaa tgcgtgctct tctcttgtca atggcaggag cagtgctgtc gctcttttca
     9421 agtatttacg ctgcagacac agtttatcta gccttggttt caatcgcggg ctttgctgtt
     9481 gttgtcgtat ggctagccat tccagtcgca caaatcaatt tccgcaagga attcttgaaa
     9541 gagaatcaac tagaagattt gtcttacaaa acacctttta caccagttct cccttatatc
     9601 acaatcattt tgctcttgat ttcgattgta gggattgctt gggatagctc acaacgtgct
     9661 gggctttact tcggtgttcc atttattatt ttctgctata tttatcacaa actacgttac
     9721 aagaaatggt gatcttatgg gaagatttaa agaactttta gagagtaaaa aaactctcat
     9781 cctacatggt gccttgggga ccgagcttga gagtagaggt tgtgatgttt ctgggaaact
     9841 ttggtcagcc aaatatctga ttgaggatcc agcagctatt cagaccattc atgaagatta
     9901 tattcgtgct ggcgctgata ttgtgacgac atcaacttat caggcgactt tacaaggact
     9961 agctcaagtt ggtgtctctg aaagtcaggc agaagatttg attcgcttga ctgttcagtt
    10021 ggcaaaagct gcgcgtgagc aagtttggaa aagtttgaca aaagaagaaa aatcagagag
    10081 aatctatcct ttgatttctg gggatgttgg tccctacgct gcctttttag cggatggctc
    10141 tgagtacact ggactctacg atattgataa gcaaggtctt aaaaacttcc atcgccatcg
    10201 tattgagctt cttttagatg agggtgttga cattctggca cttgaaacca ttccaaacgc
    10261 tcaggaggct gaggctctta ttgagctact tgccgaggat ttccctcagg ttgaggctta
    10321 tatgtcattc acctctcaag atggtaagac catctcagat ggtagtgctg ttgcagactt
    10381 ggcgaaagct attgatgtta gcccacaggt cgtggcgctg ggcattaact gttcaagtcc
    10441 atcgttagtt gctgatttct tgcaagcaat agcagagcag actaacaagc ctcttgtgac
    10501 ctatccaaac tctggagagg tttatgatgg ggcaagccaa tcttggcaat ctagccctga
    10561 tcattcacac accctacttg aaaatacaag tgactggcaa aaacttggcg cccaagttgt
    10621 cggaggttgt tgccgaacta gaccagctga tattgctgac ctctctgcac atttaaaata
    10681 agtaaagtat cttgaagaag gttagggtaa gtttgatttc ctttttcaat ccactagaaa
    10741 acccgcttgg attcttccaa acgggttttg tttttatctt tcagaaatga cgatttggcc
    10801 atcttgcaaa ttagctttta ggtgtttaac gtctgtatga tcgagatagt agtctgtgat
    10861 gcgatctcga atttcttgct cgatgacacg gcgcaatgga cgtactccca ttgcttcgtc
    10921 ataacccagt tcaattaagt gtgatttaac attttcatct accacaaggt caattccttt
    10981 tttgccaatt gtttggttaa cttcatcaag catcaaatct acaatttcgc ttaagtcatc
    11041 tttgcttagg tgagagaatt caataacacc attgaaacga tttagaaatt ctggacggaa
    11101 atatggagaa attcgttcca taattttcaa gtctttatcg ctgtcacctg taaatgcttc
    11161 gtttccaaaa cctgcatttg aggttgcaat gataacagta tttttaaagt taatagtgtt
    11221 tccttgacca tcagttaaac ggccatcatc tagaacttga aggagaagcg tgataacttg
    11281 cgggtcagct ttttcgattt catcaagaag aacaatagaa taaggatttc gacggatacg
    11341 ttctgttagt gtattactat tgtcgtcata accaacgtaa ccagcagtcg ctccaattag
    11401 cttagaaact gctgtacgat cattatattc agacatatca agtctaacga tagcatcctt
    11461 tgatccgaac atatcaaaag ctaattgctt tgctagctcg gttttaccaa ctccagttgg
    11521 tccaacaaat agaaagctac caatcggacg attaccatca tcgaaaccag cacgatttcg
    11581 gcgaatagct ctcgcaactg cctcaacagc atcattttga ccaatgactt taccttttag
    11641 acgattgcca agttctttta agcgttcaat atcactagca cccatatttg atacaggaac
    11701 tccagttaga cgctcaattg acatagcgat atcgttgata gttgcagtga ctttttgtcc
    11761 ttcggtatga ttatcaatct ggttttgtaa ctcttcaatg cgtgtcttga ccttaagagc
    11821 ttcttcaaaa tcttcagtat tgacagcttt ttcttgcttg tctctttgct cagcgatttc
    11881 tttttctaag gatttaaggt cagttacggg atgttgcgca gctaggtgtg cagcagtcat
    11941 atcaataagg tcaattgctt tatcagggag acttctttgt gggatgtatt gaatagataa
    12001 atctacagct gctttcaaaa cagagtcagg taaaacaaca ttatgatgtt gctcatagag
    12061 gtttcttatc cccataagga tattgaaagt atcttgtgca gaaggtgcat tgactttaac
    12121 ttcgttaaag cgacgtgcta aggcagcatt cttgagaatg gtgttacgat attcatcttg
    12181 agtagttgca cctataaccg ttaactcacc acgtgaaagt gcaggcttaa gtatatctgc
    12241 gagcccctta gagccactat caccaccagt tgaaccggct cctaatattt ggtgtatttc
    12301 atcaaagaag agaataatat ttcctgtttc cttaacttct tttatgatat tttgaatatt
    12361 ttcttcaaaa ctacccctat attgggtgcc agcttcaagg ctagaaatat ctatcgaaat
    12421 gatttcttta tttttgatag cagcagggac atcaccattt ataattgctt gtgccaaacc
    12481 ttcaataaca gctgttttac caacacctgc atcaccaacc agaacaggat tatttttagt
    12541 acgacgagct agaatttctg cagtttcctg aatttctttg tttcgtccaa taactggatc
    12601 tagcaaatgc tgacgtgctt gatcggttaa attagttcct aatttagtca aaacactatc
    12661 tgcagagact tggtttgtag gagtgttttg gttatttagc tcttgaccgg ggagttttcc
    12721 agtttgacga tattgactga attcttcagg agtaacttcc cgtccattga ttaagtatcg
    12781 cttattctca ctgttatagc cccccatatt tcccattaga gaattaaaaa tatcatccat
    12841 atttccaaaa gggtcacgat tataaaaatt attatttgcc atatattaaa tacctcaatt
    12901 gattttaatt tttgaaagtt aattattgat tcctatactt taatacatat agagaggtct
    12961 aatttaaata tttaattatc tttctggtac tataatacac ctttagtcag taaaggtcaa
    13021 taaataaata ctaaaatatt atttttttga tataaagata actattttag tattttttgg
    13081 tactataaaa ttatttcaga aaaataataa aaattattga caatgctaga attataagtt
    13141 atactattta aagtttctga tgaaactgac ctaagacagt aggggagcag tgctcataat
    13201 attcctaccg aggcacaaaa cgtcattata aaataacgta ttgtactttc gtgtctaggt
    13261 ttaggtgcga tttttttgtg cctatcccaa aaaataaaaa cggaggtaaa actaatgagt
    13321 gaagcaatta ttgcaaaaaa agctgaacaa gttgaactta ttgctgagaa aatgaaagca
    13381 gcagcaagta tcgttgttgt tgattcacgt ggtcttactg ttgagcaaga tactaactta
    13441 cgtcgttcac ttcgtgaaag cgatgttgag tttaaagtta tcaaaaactc aattttaact
    13501 cgtgcagctg aaaaagcagg tcttgaagac cttaaagaac tttttgttgg tccatcagca
    13561 gttgcgtttt ctaacgaaga tgttattgca ccagctaaag ttattagcga tttcgctaaa
    13621 gatgcagaag ctcttgaaat caaaggtggt tcagtagacg gtaaattcac ttcagttgaa
    13681 gaaattaacg ctcttgctaa attaccaaac aaagaaggta tgctttcaat gctcctttca
    13741 gtacttcaag cgccagtacg caacattgca tatgctgtca aagcagttgc agaaaaagac
    13801 gaagaagtgg cataagcact cttcaagtag cctagctaca taattttata ttaaaacata
    13861 tttttggagg aaataacaat ggcattaaac attgaaaata ttattgctga aattaaagaa
    13921 gctacaattc ttgaacttaa cgatcttgta aaagctatcg aagaagaatt tggtgtaact
    13981 gctgctgctc ctgtagctgc tgccgctgct ggtggtgaag ctgctgctgc taaagactca
    14041 tttgacgttg aattaacagc tgctggagac aaaaaagttg gcgttatcaa agttgttcgt
    14101 gaaatcacag gcgaaggtct taaagaagct aaagctatcg ttgataacgc accatcagtt
    14161 atcaaagaag gtgcttcaga agcagaagct aacgaaatca aagaaaaact tgaagctgca
    14221 ggtgcttcag ttactcttaa ataaaagagt accgcaattt gattgtggaa gcctagtaaa
    14281 tcacggttta aactcgattt catggtgata aaatggtggg aaaaatttta gttccccacc
    14341 acaaccccac taagttttat tcttggtggg gttttatttt tggtatttaa agatttcaat
    14401 tcaaggttat ctttatcatt gaatttcatt atactatgag tgaaatagtg gtataataga
    14461 aagcaagcac ttattttctg taataactag taattattgt tatagaatgc gcattaaaaa
    14521 tcaattaagg agttatattt gaagtcaatt gaattatttg caggtgctgg aggtctggca
    14581 ctaggtattg aaaaagcggg ttttgataca attggattag tcgaatttga tagcgctgct
    14641 tctgaaacgt tgaaatataa tcgaccaaat tggaatgtga ttcatgatga tgttgctaat
    14701 atttcaaaac ttgatttgga agaatatttt ttaattaaaa aaggagaatt agacttactt
    14761 agtggaggag ctccttgtca gtctttttct tatgcaggga aacggttagg cttggaagat
    14821 actcgtggta ctttgtttta tcactatgct gttttcttgg aaaagttgca acctaaaatg
    14881 tttctttttg aaaacgtaaa aggtttgacc agccacgata agggtaagac ctacaagact
    14941 attttgaatg tctttgagga agaaggctat acagtgcaat caaaagttct gaatgcatgg
    15001 gattatggtg ttgctcagaa gagagaacgg atgattatgg ttggaatccg taatgattta
    15061 gtaaatatga cgtcttttga atatccaaaa cctcatgatt ataagccagt attaagggat
    15121 attttacttg attgtccacc aagtcaagga atgcaatatt cagaacagaa gagaaaaata
    15181 tttgaactag tacctccagg aggatactgg aaagacattc cagagagcat tgcaaaagaa
    15241 tatatgaaat caacttggta tatgggtggt ggtcgtacgg gtattttaag aagattgagt
    15301 ttagatgaac cctctttgac tgtcctcacc tctccaactc aaaagcagac agagcgctgt
    15361 cacccactgg aagtacgtcc gttcactatt cgtgaaaatg cacgcataca aagttttcca
    15421 gatgattggg aatttcaagg aagtgtagga aatcaatata aacaagttgg aaatgctgtt
    15481 cctgtaaatc tagcttatga agtaggattg aagataaaag aaagtttgga ggtattataa
    15541 atgtggggct tatcatttat tagtgaagaa gatttttata atcatgttca attgactata
    15601 gacaagtatg gcaaaaaatt acaatccttt gatttgaaac gatttaataa aaatattgtt
    15661 gatccaataa aattgatttt tgataaatct gtttatagaa gttcgtggga acaaattata
    15721 agtaacgaga tttttagaca acgtgataaa tcaaacaata atgatattgg atattttcat
    15781 caaactattt tccaatacat agagcactgt cgagttccga ataatggaga agagggtggt
    15841 tgggatgtca tttttgaaaa tccaaatggt ataacaatgc cagatggttc tcaagtcagt
    15901 cgtgtctatg tggaaatgaa aaataagcat aacacaatga attctgcttc ttctggtaaa
    15961 acttttatga aaatgcaaaa tcagctatta caagatgatg attgtgcgtg ttttttagtt
    16021 gaggcaattg caaaaaattc tcaaaatatc caatgggagc caaaagttga tggtcgaaaa
    16081 atgggacata aatatataag acgtgttagt ttagaccaat tctattcact tgtgacggga
    16141 caggaagatg ccttctataa tatgtgcatg gttttgccag aagtcattaa taaggctgta
    16201 tcagaattag attcttcaat tattccaaat gatacagtat ttgatgaaat tagagacatt
    16261 agtgctcaac aaaactatga ttctgaagat ttatctattg caatgtcatt ctatttacta
    16321 ggtttttcta gtt
//
LOCUS       DATROY010000036        16075 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.036, whole genome shotgun sequence.
ACCESSION   DATROY010000036 DATROY010000000
VERSION     DATROY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            395..1357
                     /locus_tag="VBD11_000923"
     CDS             395..1357
                     /locus_tag="VBD11_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6953886.1"
                     /translation="MKKITKMVISSVLASVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VLFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGCQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            1373..2269
                     /locus_tag="VBD11_000924"
     CDS             1373..2269
                     /locus_tag="VBD11_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953887.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKASKTLTKEG"
     gene            2274..3077
                     /locus_tag="VBD11_000925"
     CDS             2274..3077
                     /locus_tag="VBD11_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6953888.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            3252..3536
                     /gene="groES"
                     /locus_tag="VBD11_000926"
     CDS             3252..3536
                     /gene="groES"
                     /locus_tag="VBD11_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6953889.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            3632..5254
                     /gene="groL"
                     /locus_tag="VBD11_000927"
     CDS             3632..5254
                     /gene="groL"
                     /locus_tag="VBD11_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6953890.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(5286..5650)
                     /locus_tag="VBD11_000928"
                     /pseudo
     CDS             complement(5286..5650)
                     /locus_tag="VBD11_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5663..6400)
                     /locus_tag="VBD11_000929"
     CDS             complement(5663..6400)
                     /locus_tag="VBD11_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6953891.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            6559..7338
                     /gene="udp"
                     /locus_tag="VBD11_000930"
     CDS             6559..7338
                     /gene="udp"
                     /locus_tag="VBD11_000930"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6953892.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            7359..8561
                     /locus_tag="VBD11_000931"
     CDS             7359..8561
                     /locus_tag="VBD11_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6953893.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            8591..9262
                     /gene="deoC"
                     /locus_tag="VBD11_000932"
     CDS             8591..9262
                     /gene="deoC"
                     /locus_tag="VBD11_000932"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6953894.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            9329..10540
                     /locus_tag="VBD11_000933"
     CDS             9329..10540
                     /locus_tag="VBD11_000933"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6953895.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            10710..12350
                     /locus_tag="VBD11_000934"
     CDS             10710..12350
                     /locus_tag="VBD11_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953896.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(12375..13259)
                     /locus_tag="VBD11_000935"
     CDS             complement(12375..13259)
                     /locus_tag="VBD11_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6953897.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            13303..15624
                     /locus_tag="VBD11_000936"
     CDS             13303..15624
                     /locus_tag="VBD11_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6953898.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            15673..15825
                     /gene="rpmG"
                     /locus_tag="VBD11_000937"
     CDS             15673..15825
                     /gene="rpmG"
                     /locus_tag="VBD11_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6953899.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            15861..16034
                     /gene="secE"
                     /locus_tag="VBD11_000938"
     CDS             15861..16034
                     /gene="secE"
                     /locus_tag="VBD11_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6953900.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
BASE COUNT         5203 a         2542 c         3326 g         5004 t
ORIGIN      
        1 aacaagaatt ttcaaaaaaa tcaaatttaa ctattgacaa ttatttttaa atgacgtaaa
       61 cttataggca accataaaca aggagagaat aatatgttaa tcaaatcgtc tattattaaa
      121 caaacaaata cttattatta ctttagtttc tttagataga cgtttgtagc ctcaaggata
      181 caaacgtcac gcaaagtttg tatctatgaa gctaaagcat tttatgatga aagcagagcc
      241 gattagatca tttcaataat ctagttggtt aaggcaatat tttttcgccc tcccgtgcac
      301 agaggaattg agagaaataa ttaaccaact agatgtaggg tctagttggt tttttgtttt
      361 atggaaaatt tgatggtatt acaaggagat agtaatgaaa aaaataacaa agatggtaat
      421 tagcagcgtt ttagcatctg ttctgatgac agcttgttca caatcttctc aaaatggtca
      481 atctgatgta aaacatattg gtattttaca atatgtagaa catccctctc taactgccac
      541 taggaaagga tttataaaag aattagctaa agaaggctat aaagatggca aaaatatcaa
      601 aattgagtat aagaatgctc aaggggatca gtcaaatatc caatcaattt ctgaaaaact
      661 cattaaagat aataaattag tattgggaat tgcgactccg gcggctcaat cgttgacaac
      721 ggtgtcaaca gaaacaccta ttttatttac agcagtgaca gatccggtat cagccgaatt
      781 agtaaaatca atgaaaaaac ctgaaggttt agcgactggt acaagtgaca tgtctccaat
      841 taaaaagcaa gtatcccttt tgagaaaagt aatgcctaag gttaaacgtg ttggtattat
      901 gtataccact agcgagcgta actcagaagt tcaggtaaaa caagcaaaaa aaatcttcca
      961 agaggcgggg attaaaacaa gtgttaaagg tatttcttcc actaatgatg tacaagatac
     1021 tgctaagagt ttaatgtcta aaacagaggt tctttttgta ccaacagata atattatagc
     1081 cagctcagta acgctgttag ggaatctatc gaaagaatta aaagtgcctg ttgtgggagg
     1141 ttcagcagat atggtaccaa gtggtttgct cttttcttat ggggctgatt atgaggcttt
     1201 aggttgtcaa acggcaagac aagctgttaa aatcctcaaa ggtaaagacg tcgctaaagt
     1261 tccttcagaa tatcctcaaa atctaaaagt agtagttaat gaagatatgg caaaagagct
     1321 tggaattgat gtttcaagta ttaaaaataa taaataatca gaaaggtatt gtatgttaga
     1381 tattatctta tccggaattt cgcaaggatt actttggtca attatggcaa ttggcgtgtt
     1441 tatcactttt cgtatcttag acatagccga tctctctgca gaaggggctt tccctatggg
     1501 ggctgcagtt tgcgccttat gtatcgttaa tgatattaat ccaattgtgg caaccatagc
     1561 gggcatgtta ggtggtatgt tggcaggact agtatcgggt tttcttcata ctaaaatgaa
     1621 aataccagcc ctcctaacag gtattattac gttgacagga ctttactcta ttaatttact
     1681 tgttttagga agatcaaacg tctcttttgc tctcaaaaat actttggtta caatggtaac
     1741 aagattaggt ttaaataaac tttcagcagt tttattaatt ggaattgttt gtgtaggttt
     1801 agttatctta attttgtatc tctttttaaa tacccaacta ggattagctt taagagcgac
     1861 aggtgataat gaggctatgg gacaagccaa tagtattaaa gtagatcgga tgaaaatgct
     1921 aggatatatg attgggaatg gcctgattgc tttatctggt gcacttttag cgcaaaataa
     1981 tggctatgca gatttgaata tgggagtggg tacaatcgtt attggattgg cttcaattat
     2041 cttggcagaa gtaatgataa aatacttacc tcttggtaaa aggttatggt caattgtatt
     2101 aggttctgtt ttataccgta tgattattgt tttcatatta acaaccgata ttgatgcaca
     2161 gatgataaag ttggtttcag caattttgtt ggctctcatt ttgtatgtcc cagaattacg
     2221 agctaaacta ggagtaaaag cctctaaaac gcttacaaag gaaggataag aacatgtcat
     2281 tacttgaatt agtcaatctg cacaaaactt ttgaaaaagg aactgtcaat gagaaccatg
     2341 ttttacgtgg tttagattta acgattgaag acggtgattt tatttcagta attggaggta
     2401 acggtgcggg aaaatcaacc ctccttaact gtatcgctgg tctgatccct attgatcagg
     2461 ggacaatcac attagacaac caatcaatta ctaaagactc tgtagaaaaa cgatctaagg
     2521 gtattagtcg agtctttcag gatcctagaa tgggaactgc aacaaatttg acgattgaag
     2581 aaaatatggc aattgcacat aaaagaggca ataagcgtca catttttaga cagtcagtga
     2641 ctgacgatga ccgtcaacta tttaaaaagt ctctatcaca attaggtcta ggcttagaaa
     2701 atcgaatgaa gactgatgct gcatttttat ctggcggtca gcgtcaagcc ctcacgttag
     2761 ctatggcaac tcttgttcgt cccaaattat tattactgga tgaacataca gcggctctag
     2821 atcccaaaac tagcgatatg gttatggaat taacacagaa agtgatagaa gaacaacgat
     2881 tgacagcctt aatgattaca cacaatatgg agcatgccat tgcttatggt aatcgtctcg
     2941 tgatgctcta ccacgggaaa attgttgttg atgttaaagg agaagcaaag aggaacttaa
     3001 cagttgcaga actaatggaa cttttccata aaaactcagg tcaacagttg atagatgatg
     3061 cccttgtctt aggttaaaat ataaatttct ctagtgaatg gacaggataa ctagggaaat
     3121 ttttcttgtt tgaaacgtgc cttttctctt gactaattct gaccaagtga tagaatagtt
     3181 tttgtagtta gcactctctt tataggagtg ctaaaaaatc aaaatatatc tcgtatggag
     3241 gttcgtttat tatgttaaaa ccattaggtg atcgtgtgat catttcgttt gttgaaactg
     3301 aagagaaatc tgtcggtggt tttgttcttg caggagcaag ccatgatacg acaaagacag
     3361 ccaaagttct tgctgtgggt gacggtattc gtaccttaac aggggaatta gttgcaccta
     3421 gcgttgcaga gggtgatact gttttagtcg aaaatggtgc tggtttggaa gttaaagacg
     3481 gtaacgaaaa agtgacagtt gttcgcgaaa gtgacattgt tgctgtcgtt aaataggttt
     3541 tattaagcaa gaaaggatat aggcctatta gagaaaagtt gctttgtaaa gaaagtagac
     3601 tttgttaaat tttaggcctg ggtattaaat tatggcaaaa gatattaaat tttcagcaga
     3661 tgcccgctca gcaatggtgc gtggtgttga tattttagct gatacagtca aagtaacatt
     3721 aggtcctaaa ggccgtaatg ttgttcttga aaaagcattt ggttctcctt taattacaaa
     3781 tgatggtgtg acaattgcta aagaaattga gctagaagat cactttgaaa atatgggagc
     3841 taaacttgtg tcagaagtgg cttcaaaaac taatgatatt gcaggggatg gcactacaac
     3901 tgctactgtt ttgacccaag ctattgtacg ggaaggtctt aaaaatgtaa ctgcaggggc
     3961 aaatccgatt ggcattcgtc gtggtattga aacagctgtt tcagcagcag ttgaagagct
     4021 aaaagagatt gcacaaccag tttcaggcaa agaagctatt gctcaagttg cggctgtgtc
     4081 ttcacgttct gaaaaagttg gggaatacat ttctgaagct atggagcgcg tgggtaatga
     4141 tggtgttatc actattgaag aatcgcgagg tatggaaaca gagcttgaag ttgtggaagg
     4201 aatgcagttt gaccgtgggt acttgtcaca gtatatggta actgataacg agaaaatggt
     4261 ctctgaactt gagaatccgt atatccttat tacagataag aaaatttcaa atatccaaga
     4321 aattttacca ttattagaag aggttcttaa aacaaatcgt ccgttgctaa tcatcgctga
     4381 tgatgttgat ggagaagctc tcccaacgct tgttcttaac aaaattcgtg gaactttcaa
     4441 tgtcgtagct gttaaagcgc ctggatttgg tgatcgtcgt aaagccatgc tggaagatat
     4501 tgctatccta acaggaggaa ctgtcgttac tgaagacctt ggtttagact taaaagatgc
     4561 tactatgcaa gttttaggac agtctgctaa agtaacagta gataaagatt ctactgttat
     4621 tgtcgaaggt gccggtgact catcagcaat tgctaatcgc gtagccatca ttaagtcaca
     4681 gatggaggct acaacttctg attttgatcg tgaaaaatta caagaacgac ttgctaagtt
     4741 agccggtggt gtagcagtaa ttaaagttgg tgcggcgact gaaacagaat taaaagagat
     4801 gaaacttcgt atcgaagatg cgttaaatgc aacgcgtgct gcagttgaag aaggtattgt
     4861 ttcaggtgga ggtacggctc ttgtgaacgt tattgaaaaa gtagcggcac tgaaacttaa
     4921 tggtgatgag gagactggac gtaatattgt tcttcgtgct ctcgaagagc ctgttcgtca
     4981 aattgcttac aatgctggat atgaaggttc agttattatt gaacgtttaa aacagtctga
     5041 aattggtaca ggatttaatg cggctaatgg agaatgggta gatatggtta ccacaggtat
     5101 cattgatcct gtcaaagtaa cacgttctgc acttcaaaat gcggcatctg tagcaagtct
     5161 tatcttgact acagaagcag tagtagcaaa taaacctgaa ccagaagctc ctacagctcc
     5221 tgcaatggat ccatctatga tgggtggctt ctaatcacta acttatcaca acataaaact
     5281 cccctttaac aaaggggagt tttatgttgt gataatagag atatgatagt cactaccact
     5341 tgcggtaaga agatcataag gataggccat ctaatatttg ccagtagtgg gaggaaaaaa
     5401 gtagcaaaac aatgattgct attatcttag attgagattg ataagctaag aattgaatta
     5461 tagctaatag aattaagtaa ctaataagta taaataacat agaaaagagc attccgtacc
     5521 cagcacccat tgacggttcc tttggagggg ctagaaaata acttaactta aaataagcgc
     5581 caactattag gcccataatt aattcggtaa aagttatgaa aaaagcaact tttaatagtc
     5641 tattttgcat aggcttgcct ccttaatgag aggtaatgga caattcaaag tgctcaatgg
     5701 gaacgtaatg cttattacac attagtattt tctcttggtt aaaaattgtt tcttgtatta
     5761 atatgaattg tccatgatcg gatagggtgt atttagttgc tgaaaaattg ccagatgtcg
     5821 catatcccaa atggctttgt gagtggcttg atatgccttc gtgatagata ccttcttcaa
     5881 tgaagtttaa caactgttta gtatcatgta aagaaatctc agcatctgaa ataagttcga
     5941 tagggataaa agaaagtgtg taagctaatg gtccatcatc ggtgtggtac caacgatcag
     6001 caataacaac tacaggggtt tcttgtttaa gtgaagctgt gatagcttct gcaggaacct
     6061 ctacacgaaa ttctaattcc acttctgtaa ttttggaagt tagacaagtt ttaatggggt
     6121 gttgtctatt ttcgtaaccg ttttcagaca agttgctaga attttctcta atgaaattac
     6181 cttttcctct gatattttta accaaatggt cttcttgtaa tagtgctaat gactttcgta
     6241 aagtagctcg actaacatta agttgccctg ctaattcggg ctcagaaggc aattgcattc
     6301 caggagaata agtaccgtct tgaatcatgt taaaaatctt attgtagact tgcacatgct
     6361 ttagtttatt tagtttatta tttagttcat ttttaggcat tgtaacctcc gtatacaagt
     6421 tagtttaatt gaaaaaatta gaaaaatcaa gttaaaaaat aaaacgtttt ctttcgataa
     6481 aagcgtttac aaaggttgtc ttgtatgtta ttatacaagt gtaaaaaaaa tagacaagac
     6541 attaaggagg tctttatcat gcagaattat tcaggtgaag tcggattgca atatcaccta
     6601 cagattcgtc caggtgatgt aggtcgttat gttatcatgc caggtgatcc aaaacgttgt
     6661 gcaaaaattg cagaacattt tgataatgca gttcttgtag cagacagccg tgaatatgtt
     6721 acttatacag gtacattaaa tggtgaaaaa gtcagtgtta cttcaactgg tattggtggt
     6781 ccatcagcgt ctattgcgat ggaagaattg aaactctgtg gcgctgatac ctttatccgt
     6841 gttggaacat gtggtggtat tgaccttgat gttaaaggcg gagacatcgt tattgcaact
     6901 ggagctatcc gtatggaagg tacaagtaaa gaatatgctc cgattgaatt tcctgctgta
     6961 gctgacttgg aggtaacgaa tgctctagtt aatgcagcta aaaagcttgg ttacacaagt
     7021 cacgcaggag tagttcaatg taaagatgca ttttatggac aacatgagcc agaacgtatg
     7081 ccagttagtt atgaattatt aaataaatgg gaagcatgga aacgtctggg aacaaaagcg
     7141 tctgaaatgg aatctgcagc tctttttgta gcagcaagtc atctaggagt tcgttgtgga
     7201 tcagatttcc ttgttgttgg taatcaagaa cgcaacgctt tgggtatgga taacccaatg
     7261 gctcatgaca cagaagcagc tattcaagtt gctgttgaag cactccgcac acttattgaa
     7321 aatgataaat cacaataagc gaggttttgg aagtcatcat gcaatttatt tatagtatta
     7381 ttggtatttt attggtatta ggaattgtgt atgcaatttc tttcaatcgt aagagtgttt
     7441 ctctaagttt aattggaaaa gctcttatcg ttcaattcat tattgcgcta atcttagtac
     7501 gtatcccact aggccaacaa gttgttagtg ttgtttcaac tggagttact aaagtaatca
     7561 actgtggtca agctggttta aattttgtgt ttggttcatt agcagatagt ggaacaaaaa
     7621 ctggttttat tttcgccatt caaacgcttg gtaatattgt tttcttatct gccctagtta
     7681 gtctacttta ttatgtagga atccttggat ttgtagtaaa atggataggt aagggtgttg
     7741 gtaaaattat gaaatcctca gaggttgaga gttttgttgc cgtagctaat atgtttcttg
     7801 gtcaaacaga cagtccaatt ttggttagca aatacctagg tcgtatgact gatagtgaga
     7861 taatggttgt gttggtatca ggtatgggaa gtatgtcagt ttctattctt ggtggctaca
     7921 ttgcattagg cattccaatg gaatatctct tgattgcttc aacaatggtt cctattggca
     7981 gtattctcat tgctaaaatc ttatttcctc aaacagaacc tgttcaaaaa attgatgaca
     8041 ttaagatgga taataaaggt aataacgcca atgtgattga tgcaatcgct gagggtgcaa
     8101 gcacaggtgc acaaatggct ttctcaattg gtgctagttt gattgccttt gttggtttag
     8161 tttctttgat taatatgatg ttaagtggat tgggaattcg cttagaacaa atcttctcat
     8221 atgtttttgc tccatttggt tttcttatgg gatttgacca caaaaacatt cttctagaag
     8281 gaaaccttct tggaagtaag ttgattttaa atgagtttgt ttcgttccaa caattgggtc
     8341 acctaatcaa atctttagat tatcgtacag cattggtagc aactatttca ctctgtggtt
     8401 ttgctaattt atcaagttta ggtatttgtg tttcaggtat tgctgttctt tgtccagaga
     8461 aacgtggcac cctagctcga cttgttttcc gtgcaatgat tggtggtatt gctgtaagta
     8521 tgcttagcgc ctttatcgtc ggtattgtaa ctctattcta aaagttgaca aaagaaagaa
     8581 ggatagtaac gtggaagtaa aagatatttt aaaaacggta gaccatactt tgctagcaac
     8641 aacagcaaca tggccagaaa tccaaacaat tttagatgat gcaatggctt atgaaacagc
     8701 ttcagcatgt attccagcct cttacgtcaa aaaagcagca gagtacgttt caggtaaatt
     8761 agctatttgt actgttattg ggttcccaaa tggctatagt acaactgcag cgaaggtttt
     8821 tgaatgtcaa gatgctatta aaaatggtgc tgatgaaatt gacatggtca ttaatttgac
     8881 agacgttaaa aatggggatt ttgatactgt tgaagaagaa attcgtcaaa tcaaagctgc
     8941 atgtcaagac catatcttaa aagttatcgt tgagacatgt caattaacta aagaagaact
     9001 tatcgaactt tgtggagttg tcacacgttc aggtgcagac tttattaaaa cctctactgg
     9061 tttttcgaca gcaggtgcta catttgaaga tgttgaagtg atggcaaaat atgtcgacga
     9121 aggtgttaaa attaaggcag caggtggaat ctcatcattg gaagatgctg aaaaatttat
     9181 tgctttagga gcttcacgct tgggtactag ccgtatcatc aagattgtta agaaccaaaa
     9241 agttgaagaa ggcacttatt aaataatata aaggaaatag gaaggatgct ggtctgactt
     9301 cctattttca attgataaag gagaagttat gtcacaattt gatcgcattc acttagttgt
     9361 cttagattca gtgggtattg gagcggcacc agatgccaat gattttgtca atgcaggagt
     9421 tcctgatggt gcttcagaca cgcttggcca catttcaaaa acagttggac ttgcggtgcc
     9481 aaatatggct aaaattggac ttggtaatat tcctcgtccc caagcgctta aaaccgttcc
     9541 tgcagaagaa aatccaagtg gctacgcgac gaagttacaa gaagtatcac ttggtaaaga
     9601 tacaatgaca gggcattggg agatcatggg cttaaatatt acagagccat ttgatacttt
     9661 ctggaatggt tttccagaag atatcatcac aaagattgag gatttttcag gacgtaaagt
     9721 tattcgtgaa gcgaataaac cttattcagg gacagctgtt attgatgact tcggaccacg
     9781 ccaaatggaa actggtgagc tcatcatcta tacctcagct gacccagtcc tacaaattgc
     9841 agctcatgag gatatcatcc ctctagaaga actttaccgt atttgtgaat atgctcgtag
     9901 tatcacaatg gagcgtccag ctctccttgg tcgtattatc gctcgtccat atgtaggtga
     9961 cccaggcaac tttacgcgta cagccaaccg tcacgactat gcagtatcac catttgaaga
    10021 taccgtatta aataaactag atcaagctgg catcgatact tatgctgttg gtaaaatcaa
    10081 tgatattttc aatggttcag gcattaatca tgatatggga cataacaaat cgaatagtca
    10141 tggtattgat actttgataa aaacaatggg gttatcagag tttgaaaaag gcttctcctt
    10201 tactaatttg gttgactttg atgctctcta tggtcaccgt cgtgatcctc atggctatcg
    10261 tgattgtctt catgagtttg atgagcgcct cccagagatc atttcagcaa tgcgtgacaa
    10321 agacttactt ttgatcactg ccgatcatgg taatgaccca acttatgcag gtacagacca
    10381 tacaagagaa tacattcctc tcttagccta tagcccatct ttcacaggaa acggccttat
    10441 cccagtaggg catttcgcag atatttcagc aacagttgcg gataactttg gtgttgatac
    10501 tgcgatgatt ggagagagct tcttgcagga cttggtataa tcatagacaa aaattttaga
    10561 ggttgaaatc ggattatggt ttcaatcctt ttttcgtttt gttataatag aaaaatagcg
    10621 gttacaagta caaatacagc tgttttatca gtgttgtaac ttttctttaa gcaatttact
    10681 ttaatataaa gtgaatgaga ggataaggta tgtttgattt ttttaagaaa aaagttgtta
    10741 aagtgtgctt agttattttt ggtatagttt tagtatcact tctgagtcta ggattctttt
    10801 atttttcaaa gggacaagta cttagtcgtt ttgtagcagc acgttctcgt acgtcaggtc
    10861 aagcgtttga taatattaaa gaatacatgg tttggtcaga tacgggagaa tctatcacaa
    10921 atgatgaggc caattacgct aattttgagc ctttgtctaa aagtgaggcg cgtaaattag
    10981 ggcaggaaat aaaagaagga aacaaaaatg attcaatgta tctaaagcgt gtaggtagtc
    11041 gcttaggtat attccctgat tatcgtattg ctaataagcc tatgtctttg accttgaaaa
    11101 ccaatgttcc taaattggat gttttgttga atcaaaaaaa ggtcgcgaca agcaattcag
    11161 atcatttttc agtaacggtg gaacgtttac ctagaaccca ttatactgct agccttgaag
    11221 ggactagtga cggaaaagag attaaactca aaaaagatta tgatggtaaa aaccaaacta
    11281 ttgatttatc ggtcgctttt aaatctttta cagtaacaag taatcttatg gatggtaatc
    11341 tttattttgg tgataatcgt attgctaaat taaaagatgg tagctattcc gtagagaatt
    11401 atccagtgac tgatggttca aaagcttata tcaaaaaagt ttttaatgat ggtgagataa
    11461 cctctcataa acaaaaatta atctctatcg ctgataatca gacaatcaaa ttagatgttg
    11521 atggtttatt aaatgaaaaa gaagctggac agaaattgat aactgctttc aatcaattga
    11581 tactttatgt atcaactggg caagaccctc agactttagg aacagttttt gaaaaaggtg
    11641 ccgaaaatga tttttacaaa ggattgaaag aaagtattaa agcaaaattt gtaactgata
    11701 atcgtaaagc gagccatttc actattccta atattgtcct taataaaatg acacaggttg
    11761 ggaaagaaag ttatcaagtt aattttgctg ccgattatga ttttaattat gataaatcaa
    11821 cagatcctga taagaaaaca tatggccaca ttattcagaa tttaactggt aattttatta
    11881 tgaaaaaatc gggaaatagt taccttattt ctaatgatgg taaaaaagat atcacagtag
    11941 ctaaggaaac taataaagtt aaggctgatc ccgtttctat ttttccagag aaccttgtag
    12001 gaagttggaa aggtgaagtt gaggacggta cagtaacgat gacttttgat aaggatggta
    12061 aagttactca gaaaaaagtt tacaaggata gtaaatctaa ggaaagtaac cattctgcta
    12121 aagttacgaa gttggaggat aagggaaatg ggctctacct ttatcaatat gaatcaggta
    12181 ctgatactac aacttttgta acaggaggta tcggtggtct taaagttaaa tacgcctatg
    12241 gaataaaaat tgaagggaat aagatcatcc cagtaatctg gcagacatct agtgatggag
    12301 aatttgatta ccataaacca ctactcagta agccattaac taagcaataa tgattaaaag
    12361 gagttggata attattatcc aactcctttt ttataattgt ttaaaacctt ctcgaaagta
    12421 gatgaagggg cctctacgct aatagtttcg caagttaatg gatgggatag agtcaatcgg
    12481 tgagcgtgta gcattaacct ttcattatta gaagaagggt tgtagagggg atctcctatt
    12541 aaaggatgtc cgtgatgaga taagtgaatg cgaatttgat gggttcttcc ggtttccagt
    12601 cggcatttta tgagactact attttggata tacttcaaaa cgtcaatgat tgtcatagca
    12661 tgttgaccgt ttttagaatc aatgattcgt ttacgtctgt catgacggtt ccgtccaatt
    12721 ttgtctctca aaacttgatg cttaggtgaa aattttcctt caactaaagc ccaatattca
    12781 cgccaaattt cttttcgttc taagcgttga ttgataaggg gaagtataaa tggattttta
    12841 gcaaataaaa cagctccact ggtctccata tctaggcgat gaacaacata gcatgtttgt
    12901 ccagaatagg cagatacatg atttaacagt gctatttcat ttggttggtt accgtgagtt
    12961 ttcatacctt caggtttatt aacgataata agatgttcat cctcataaag acaatcaata
    13021 agctctgctc tgcccagagg aattttttta gtagggtaat cctcatcatc aaagattaag
    13081 gtaatagtat cgttttcttg gacgacagtt tgccaattaa tgaattcatt gtttataagt
    13141 acatgttttt taacacgcaa aaaatgtcta atcttacgtg gaatcaaaaa ataatcttca
    13201 aggagttctt taacggtatg ttcaccgtat ggatttctaa tttttaatga aaatgacatg
    13261 gttttattat agcattttat tgaaaattga tataataagt ctatgaaatt atttgataag
    13321 tttatagact tatttcgtgt tgatgaagac aacgacgaaa tgacaagaaa aaatgaacaa
    13381 gaaactagag aagaaactag caatttggat ggcgaagaag tctatgatat tgatgatatt
    13441 acccgaccta gtaaatccca gtatcagcga ggcattcgtc accaaaagga aaatgctaaa
    13501 tctcgacttg aatggctaca aaaagttgat cgttacctcc cttctcctaa gaatcctatc
    13561 cgtcgttttt ggcggcgcta tcgtattggg aagttgcttt ttatcgcctt aatggctttt
    13621 attcttatat ttggtagcta tcttttttat ctttctaaaa ctgctacagt tagtgattta
    13681 cagagtgctc taaaaacaac caccaccatt tatgataaga ataaagaata cgctggaaaa
    13741 ctttcgggac aaaagggaac ttatgtagag ttaaatgcta tttctgatca tctcaaaaat
    13801 gctgttatag caactgaaga tagaactttt tatgaaaata atggtgtaaa ctttaagcga
    13861 ttttttttgg cagtagctac acttgggaaa tttggcggag gctcaactat tacccagcaa
    13921 ttagcaaaaa atgcttactt gtcacaggac caaacgatta aacgcaaggc gcgtgaattc
    13981 tttttagcgc tagaattgac taagaaatac agcaaggctg agattctgac catgtatctt
    14041 aataactctt attttggtaa tggtgtttgg ggtgtggaag atgctagtag gaaatatttt
    14101 ggaaccagtg ctgctaattt gacagtagat gaggctgcta cacttgctgg aatgcttaaa
    14161 ggaccagaag tttataatcc ttattattct gttgaaaatg caactaaccg aagagatact
    14221 gttttggcag cgatggttga tgcaggtcaa ctaaccaagt ctcaagctaa agaagctgct
    14281 tcaattggta tgaaaaatag attagcagat acctatgctg gtaaaattaa tgattacaag
    14341 tacccatcct attttgatgc tgttgtcaat gaagccattg atacttatgg tatttcagaa
    14401 aaagatattg tcaataatgg ctataagatt tacactgcat tagatcaaaa ttatcaatca
    14461 ggaatgcaaa aaacatttga tgatactagc ctcttccctg tatctgacta tgatggtcaa
    14521 tctgcccaag gtgctagcgt agctctagat ccaaaaactg gtggtgtaag aggtttagtt
    14581 ggacgtgttc aaagtactaa agatgcacag tttagaagtt ttaactacgc gactcaatca
    14641 aagagaagtc cagcctcaac cataaaacct ttagttgttt attcaccagc tattgcttca
    14701 ggatggtcaa tagataaaga acttcctaat aaagttcaag attttcatgg ttataaacca
    14761 agtaactatg gtggaattga gactgaaagt atcccgatgt atcaagcact agctaattct
    14821 tataatattc ctgctgttta tactttagat aaactaggaa tcaataaagc ttttacttat
    14881 ggcaggaagt ttggtctaaa tatgtcatca gcaaacaaag agctaggagt ggcgctaggt
    14941 ggaagtgtaa ctactaatcc tttagaaatg gcacaagctt actcaacttt tgcaaatgat
    15001 ggtataatgc atcgcgctca tttgattact cgaattgaga cagctaatgg aaaattagta
    15061 aagcaattta cagataagcc taagcgagtt attagtcgct cagtggctag taaaatgact
    15121 agtatgatgt taggaacatt ctcaaacggt acagctatta atgccaatgt ttatggttat
    15181 acaatggcag gtaaaactgg aacaaccgaa acagatttta accccaattt atctggagac
    15241 cagtgggtag tcggctatac gcctgatgtc gttattagtc aatgggttgg ctttaagaac
    15301 acagataaac accattattt gacagattct agtgcaggta cagcttcaaa tatatttagc
    15361 acgcaagctt catatatact accgtatact aaggggtcta gttttactca tattgagaat
    15421 gcttatttcc aaaacggtat tggttcagtt tataatgcac aagatgctag taatacaacg
    15481 aaccaagaat cacgctcaat tattaatgat ttaaaagatt cggcaagtaa ggctgcacaa
    15541 gatatttcta gagctgtgga agattcaaat ttccaagaga aggtaaaaga tgcttggaat
    15601 agtctaaagg actactttag atagcttgca ggattataca aatcatgtta aaataacact
    15661 tatggaggcg ttatggcaca aaagaaagca agcctagcgt gtacagaatg cggaagccga
    15721 aattactcaa tcggagtaag tagcaccccc aagccaacac gactagaagt aaataaattt
    15781 tgtaaatatt gtaagaaata tacaatacac aaagaaacgc gttgatatag cgtaacaaac
    15841 tgcaaaaagg agaactgata atgtttgtca aagggatttt tcaagtattg cgtgacacta
    15901 cttggccaaa tcgaaaacaa cgttggaaag attttatttc catcttggaa tacactgtat
    15961 ttttcacaat cgttatctac atctttgaca aactgttagc tgcaggagtc atggacttga
    16021 ttaatcgatt ttaataatag attattttag atagaaacaa cccaaaccct tagat
//
LOCUS       DATROY010000037        15805 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.037, whole genome shotgun sequence.
ACCESSION   DATROY010000037 DATROY010000000
VERSION     DATROY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15805
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..908)
                     /locus_tag="VBD11_000939"
     CDS             complement(<1..908)
                     /locus_tag="VBD11_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6953901.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIR"
     gene            complement(1017..1946)
                     /locus_tag="VBD11_000940"
     CDS             complement(1017..1946)
                     /locus_tag="VBD11_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6953902.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(1943..2800)
                     /locus_tag="VBD11_000941"
     CDS             complement(1943..2800)
                     /locus_tag="VBD11_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6953903.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(2803..4158)
                     /locus_tag="VBD11_000942"
     CDS             complement(2803..4158)
                     /locus_tag="VBD11_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6953904.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(4171..4455)
                     /locus_tag="VBD11_000943"
     CDS             complement(4171..4455)
                     /locus_tag="VBD11_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6953905.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(4458..6494)
                     /locus_tag="VBD11_000944"
     CDS             complement(4458..6494)
                     /locus_tag="VBD11_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6953906.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLTAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETISLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKNPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(6710..8335)
                     /gene="treC"
                     /locus_tag="VBD11_000945"
     CDS             complement(6710..8335)
                     /gene="treC"
                     /locus_tag="VBD11_000945"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6953907.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(8557..10587)
                     /gene="treP"
                     /locus_tag="VBD11_000946"
     CDS             complement(8557..10587)
                     /gene="treP"
                     /locus_tag="VBD11_000946"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6953908.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGSIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(10869..11495)
                     /locus_tag="VBD11_000947"
     CDS             complement(10869..11495)
                     /locus_tag="VBD11_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953909.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(11479..12282)
                     /locus_tag="VBD11_000948"
     CDS             complement(11479..12282)
                     /locus_tag="VBD11_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953910.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(12294..13115)
                     /locus_tag="VBD11_000949"
     CDS             complement(12294..13115)
                     /locus_tag="VBD11_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953911.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(13112..14089)
                     /locus_tag="VBD11_000950"
     CDS             complement(13112..14089)
                     /locus_tag="VBD11_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6953912.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(14202..15692)
                     /locus_tag="VBD11_000951"
     CDS             complement(14202..15692)
                     /locus_tag="VBD11_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6953913.1"
                     /translation="MGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKK
                     YKISKDGLTWSFDLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGK
                     NQVNIHLTEAHSTFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVR
                     NPYWHGKKPYFKKWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASN
                     DVRGLSLPYVKKGVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKP
                     AYSIIDRTPFWNPKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTN
                     DQLRANLAVEVAEQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHY
                     PSLAGKGWTNITFYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPN
                     VWLVSLNHTYIGDKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
BASE COUNT         5227 a         3238 c         2511 g         4829 t
ORIGIN      
        1 tctctaataa atgaaatgaa gggagtgatg ccgatacctc ctgcaatcca aacctgttct
       61 ttcttgtctt tatcaaataa catatgacca taagctctat ctaaggctat ttttgtccca
      121 acttttagct gtttgtaaat ggatttcgta taatctcctg aagctttaac agttaaaaat
      181 atcactctat catgaccacc cgatatcgag aaaggatgag ctgctgattc aaagccggct
      241 tgataaattt ttaaaaaagt aaattgtcca tagtcatatc gataaggacg cttcattgct
      301 atttcaatct cagttgtatc atggttaaga tgagtaacct tttgaacgta ccctacacga
      361 cgaaaacgca ttctagaata taggaaaata atataaaatc cagatataac cccaatgaca
      421 gcataaccta atacgattaa actgagtaaa gtattcccta gtatcctatc tcctaaaatc
      481 agaaaagtat gtaccaatcc caaaatatag actaaataaa caaagcgatg aataaaacgc
      541 caaatttcat actttaaaaa atttccgaaa taagctacaa aaacaatact tagaaaaaga
      601 tataatcccg cactgccaat agtctttgca aactcacttc catgtccgcc ttgtacaaga
      661 ccaattttgt gcaatagaag caaaatcatt gaaaatacag ccattacctt gtggtaaaaa
      721 tacatatttt caataccttg aaagtaactt tcaagtatcc taaatttggt tgataagagg
      781 aatgttagcg ataaacttgt caaagctaat cctggtacaa caaattcact taatcctgca
      841 ttttgccatg ctactagtgt caccactatg ctagataaaa ttaaaacaat acctttccaa
      901 gctcgcataa cgaccacccc tttattttta gtaaggataa aataccacaa gatacttata
      961 caaaacttaa aaatttcaga aaataacaaa aaggcaactc caatgttgcc tttttgttat
     1021 gatttataaa ttgatttgca atgctctact atatctttaa cagcgagtcc atactcctct
     1081 aataaatatt ccatctgacc tacctgacca aatcgctcat caatacccat acgactaaca
     1141 gtagtgtctt tctccatact aagccattcg cataatgcac ttccgatacc gccaatacgg
     1201 ttatgattct caatagttac aatggattga tctatcagta atggtttcag ttcttctgga
     1261 aggggcttaa ttttaaaaag gtcgataact gaggcctcaa tccctagttc tttgaggtag
     1321 tcggctgctt ctatcgctct agaaaccata ataccacttg ctacaagagc aatatctttt
     1381 ccctggcgta attgtataaa accttttgaa aaatcttcgc atccctcata aactgcagtg
     1441 ggggccttac gaatagttct aatgtatttt agaccatcaa tacttaatgt ttgttttaaa
     1501 attgcttcaa actgaatatc atcactaacc tcaaaaatag tcgcctttgg aattaacctt
     1561 agtagaccta actcttcaaa aggcatatga gttccgccat tcatttctgc gctaactcct
     1621 gcatctgagc caataatcgt agctgataat tgtgagtaac ctaacgataa aaatacttga
     1681 tcaaagacac gccttgacgc gaaaggacca aaagtatgaa ggtatggctt gtaaccttta
     1741 atggcgagcc ctgctgcaag acctaccatc tcagcctcca taatacctag attaatataa
     1801 cgttttccaa attctgatgc cagtacattt gttgacattg aactcgacaa atcggcttct
     1861 aaaacggtaa tttgtttatt ttcttgatta gcttgaagga ggaaatcacg atagacaagc
     1921 ctcatttctt tggttgaaca tctcattcta ccacctccaa actttctctc aagcttatca
     1981 ccgctcgctc taacattgtt ttttgttgta aatctggtcg taaatgatga ttagaagcta
     2041 actcctccaa ctctttcaca ccttgtccct tgatactatc taaaacaatg cattttggtt
     2101 tgacactatt tgaatcttga aaagttttaa tagctttgtc aattgcctca atgtcatctc
     2161 cttttacacg tactgcatca aatccaaaag cttcaaattt agcaacaaag tctccaggat
     2221 tacaaatatc agctgtcaaa ccatctaatt gttttttatt atcatctaca aaaacaatta
     2281 aatggtgcag ttgatgatgc gcagcaaatt gtatagcctc ccaacattgt ccttcattta
     2341 attcaccatc acctacaata gtataagtat aatagcttga attctcaatc ttttgagcat
     2401 aagcaattcc cgttgcaata ctaatgcctt gacctaatga gcctgtcgtt acatctatac
     2461 caggagttaa attgcggtca ggatgcgaag gtagtttggt accgtttgta ttgagcgaat
     2521 gaaggaatgt tttatcaaaa aaacctttta aatagagtgt actatacaag gctggaccag
     2581 catgtccttt tgataagacc atataattcc gatcactttc tttgaatttt tcagggttta
     2641 tatccataat atcaccatac agaatagcca aagtctcaac aattgataaa ctacccccat
     2701 agtgaccgaa tcctaaatgg tttagcgttt ctagtgtatt taaacgaatc tccgttgcaa
     2761 atcgtcttag agatgaatat ctttcttctg gtgatgtcat tattaacctt ccttatgggt
     2821 tgattttcca acaaatgaaa tgccaaaaag tccaattaag ataactacaa taccaataac
     2881 aatagctatc gcaccgccga catgattaag agcgccaaga atgataccgg tcaaaccaaa
     2941 atcagcatct gagaatgttg aacctttgaa accaagtcca cctagtacag gcatcaagaa
     3001 aatcggcaag aaactgatga ggataccttg aacaaatgca ccaatagtcg ctcctctcac
     3061 accaccagaa gcattaccga taacacctgc cgttgcccca cagaagaaat gaggaacgac
     3121 ccctggtaaa atgacagtag tgccagtcac aatcataact atcatactaa ccaatccacc
     3181 aacaaaactt gagataaaac caatcaatac tgcattaggt gcataagggt aaacaattgg
     3241 acaatccaag gctggttttg aatttggaac caatttttct gagattccct taaaggctgg
     3301 tacaatttca cctaggataa ggcgtacacc tgctaaaata acgaatacac ctgctgcaaa
     3361 ttgcccagct agttggaggg cataaacaag accattgaca ccgttactta tttcttttgc
     3421 aatataagcc tcaccagcaa acactgcaac gataaggtaa ataattgcca ttgagatagt
     3481 aatacttacg gtactgtcac gtaaaaatga taaactcttt ggaaatttaa tatcttctgt
     3541 tgattttgat ttatctccaa caattccacc aataaatcca ctcaaccaat agcctaaaga
     3601 accaaagtgc ccaagtgcca ccttatcatt acctgtcagt tgaatcatgt atttttgcac
     3661 aaatgcagga gatacgctca taataatccc taacgctaga cttccaaaaa ggatgagcga
     3721 gaacgaagta aagcctgcta cggcaaaaat aacagcaatc atacaagcca tatacaatgt
     3781 atgatgtcct gtcagaaaaa tatatttaaa ttttgtaaaa cgagcaatta aaatattaaa
     3841 aatcattccc gctaacataa tcaaagcagt tgctgagcca tacttcgtaa gagctactgc
     3901 tacaatggct tcattattag gaactacccc caccaaatga aaagcatgtt caaacatttt
     3961 tccaaatgga ttcaaggaat tttggactat ccctgcacct ccagaaacca ctaagaagcc
     4021 aacaaatgtt tttattccac cttttacaat atcaggaaca ccttttttct gcagtactaa
     4081 accgataatg gctatcaagg cgactaaaat agctggcgtt ctggcaatat taactaaaaa
     4141 atccaataga cctttcatgg catcaacctc ctagattaag cctttttctt ggcaaatacc
     4201 tgtcactaat tctctcaatt catccatatc aatgatactg tttaaaatac gaacatcccc
     4261 caggtgacca gctgaatcct ccaaatcacg accaacaatc caaacatctg ctgcgcttgg
     4321 atctgctcca cctaaatcat aatgttcaac ttcaacatca gaaaccccca aatccttcaa
     4381 aatagactca atattcattt gtaccataaa acttgaacct agacctgatc cacatgctgt
     4441 tccaattctt aacatcttta atcctcctgt tttataatca tttcaatatc ttgataccca
     4501 cgtgaagcta acaaagcttt aacattatta ttatcacgaa gtattgttgt caaatgcgat
     4561 agagctttca aatgcgtttc attatcaata gctgctatgc ataataagag ataaatttcc
     4621 tgtttggggt catccaataa ataaataggc tgttccaaaa caagcattga cataccgaca
     4681 gagtttaccc catcttctgg tctggcatgt ggaatagcaa tacctttacc aagattgata
     4741 aaaggaccaa actcttcgac cttttcaatc atagcttctg ggtaactttc cgtaattttt
     4801 ccagaagcta acaagggttt tgccgctaaa cgaattgcct ctttccaatt catttgttca
     4861 gaacttgtct gataagtttc ctctgttata agttggtgca acaagggcct cacatccttt
     4921 ctaaatatat cttgttttac cattgtcctc aaagccaatt ttaattcctc ttcacaatga
     4981 acatgagcgt atttttttat agttgctatt aattgatcaa gttggaaatc gtcaaggcaa
     5041 gctttgggaa aatctgaaat aaccagttct ttgagctgtt gtacttgctc tgctgtcatc
     5101 atcagcgaaa ctagataatt agggtttttt gtttctacaa atatagtaga aaagaccata
     5161 tcgtaagttt gattgtcaat cagtttcagc tgctcaattt tagagacacg atgaaaatga
     5221 atttgaggaa ataatccacg caatttttcc ttaatcacta aggatgaact aacgccgttt
     5281 ggacagagta tcaaagcttt ataagacatt gattgtgtat cacctgattg tctaagatag
     5341 cctccaaagt gaatcacgaa gtaagaaatt tcactatcgg gtattaatcc tacttgctct
     5401 tccaaaggac gtagtgcctt tttaaccaat agaaataaat cactgtaatg ctctttaata
     5461 ttttgtgtat aaccactatc gccagttaaa ccatatttca atcggaaata agctggaata
     5521 atatgacgct tcagaccttg taataactct tctttattgg aaaaattaag aagtgaaata
     5581 gtttccatct catcaacgat tgcttttgcc aatgcttcaa agaaatcatc atctttagtt
     5641 ccctcacctt caaaacagcc agataagatc aaggtaatat aatctttttc tttctgatct
     5701 aatggtttag ataaggaaat atcctgactt aaattagtca caataatagt cgttaactct
     5761 tttaattgtt cagaaacaat aggagaaccc tgcaaaacac gatctactga cctttgataa
     5821 cgacacagta ttaaaatgat aaaatataaa gatttctcaa gcctatcttg tattggagaa
     5881 agttgaaaag attcatagaa atattctacc gtcttttcta acttctcata tgagagagcg
     5941 aatttccaag aagacaagat ataattcaaa gaccaaaaac caatgggaga ttctagtagt
     6001 tgagttatca tctgaaaagc aacttgatgc ttatccatct catctcctac tagactatat
     6061 ccctttgctc ttgtataagt gagtgagata cctctttttg ctaatttaga acgtagcatc
     6121 ttgatatctg ataaagtagt attcttactt actttcaaaa aatcttgata atgcacattg
     6181 gagacaaact ctcttcggca aaagctataa aggtaaatca gacataccct ttcttcctga
     6241 gttaggtaaa attgctcagc ttctaataag gagacaattt tatcatgctc tttttcatct
     6301 cctaataact gataacgtcc agcagagtga gcaatagctg ttaggtgatt ttttgctaaa
     6361 aaactattga gatagttaat ttgctctatg ataactttta ttgaaaactt tgtttgcact
     6421 gttaactcat acaaggaaag atttttccga gctaaaaata ccaacattat cacaacagtc
     6481 ttattatcaa ccatttgttt tcctttctat ttttatttta tagtaaccgt ttacattttt
     6541 ctaggtcatt tgagcccaat ctggcactac tatcaattga atgaatttgg gctattgtta
     6601 tagagtaaag aaaaaagaaa gaaattgcaa aatctagagc aagattcctt cccacaagca
     6661 gcacttaaaa aacctttact taattgaata agtaaaggtt ttttaaatgt tatgacacta
     6721 agatagctaa agtttgataa ggttgcaaag taaccgtttc atcaagagta acatccttat
     6781 aatttgatag aagaacttgt ccttggagat aattctccgg taattttatc ttaactttct
     6841 cagcaaagaa attatttaaa acaagaagtt tttccttatc aagatggcgt tcaaaagcat
     6901 atactttttc gttatctttg aaagctgctt tataattccc gtcagctatt attggcagtt
     6961 gtttacgtag ccttatcaac tcttgataga aagtaaatat cagccctgtt ttctcctttg
     7021 cgacattgat ttctttatat gattttccta ctttcaacca tggagcgcct tcagaaaaac
     7081 ctgcattcgt actatcatcc cattgcattg gtactcgaga attgtctcga gatttcgctc
     7141 gaataatgct aaatgcctct tcttgtgact tgccttcatc aagcattatt tggtaggcat
     7201 tcaaactctc aatatccaca taatcatcca tagaactgta atcgggatca agcattccga
     7261 tttcttcacc catatatata taaggagtac cacgcgataa gtggatagat gctgctaaca
     7321 tggttgctcc ttcatttcga aaacgtttca cgtctacaaa acgatttaat gctcgcggtt
     7381 ggtcatgatt attatagaat aaagcattcc aaccatttcc ttcgctcatt ccttcgcccc
     7441 aactatggaa aaggtcacgt aatgcaggaa aatcaaaagc cataatggtc cacttttgcc
     7501 catctttata atctactttg aggtggtgaa aattaaaagc catggataac tcttcacgtt
     7561 ctggtgcagt gtaaagaatg caatttgcaa ttgtcgttga agacatttca ccaactgtca
     7621 tgaaggaatc agcttgtcca aaactagcat tattcaacat cttgagataa tcatgtgtta
     7681 ttggacgatc agtataagct ggttttccat cattaattgg gcagttctca agtatctcat
     7741 ctttcccaat taaattaatc acatcaaatc gaaaaccttt aaccccttta tcacgccaaa
     7801 aattgacaac tttaaaaagt tccttgcgaa catcggcatt gcgccaatta aggtcagctt
     7861 gagtgatatc aaaaagatga agataatact tccctgtatc accaaatggt gaccaagcat
     7921 ttccaccaaa tttagatacc caatctgttg gattatcacg taagataaag aaatcttgat
     7981 agtagcgatc tcctgcaagt gcttttttaa accactcatg ttctattgaa caatgattaa
     8041 gcaccatatc aagcataaaa tctatacggt attgacgtcc cacctcaatc atttcttcaa
     8101 aatcgtccat cgttccaaaa tcagggttaa tggctgtata atcagagata tcatatccat
     8161 tatctcgttg tggacttgga taaaatgggt tgagccaaac catatcaata ccaagttctg
     8221 ctaaataggg taatttttcg ataataccac gaagatcacc aaccccatta ccagttgtgt
     8281 ctttgtatga ttttggataa atttgataga caactttacg tttatcaatc gtcatattct
     8341 gctcctttcc caaaatgctg ctgtatttag cagtctaact ataacatcaa ctatcatcaa
     8401 aaatataaca tttttacagc gatatttgtt cccatgagtt ttttcttatg ttcaaaataa
     8461 ttcaaggagc taatgtatta agcccctcaa acaattcctc aaattacctc tcattttata
     8521 agagtgctct tttttaaaaa ggatacctaa ttacttttaa attttcttgg caaccattaa
     8581 tgcttgtcca cgttttatct gtttgggtaa atctatcaat tcttctggtc ggaattcttc
     8641 ttgattagta atcagaatag gagtttccac aatgtatcct tcttctttta tcattggaat
     8701 atcgaaagta atgagtttat ctcctacttt tacattgtca ccttgggcaa catgagaggt
     8761 aaaccctttt ccttctaaat taacagtatc cataccaata tgaataagaa actcaacacc
     8821 ctctgatgtt aataatccaa tagcatgttt cgtagggaaa agaactgaaa ctgtcgcatc
     8881 gaccggtgaa actagttctc catcacttgg atcgataacc actcccttcc ccataattcc
     8941 ttgtgaaaag acaggatcac tagcttgatc cagtggttta gctaaacctg ataaagggct
     9001 tactacacta agctttgtac ctgagacgac agctttttct tttgctgatt ttgtttctgt
     9061 tgttgaagca atgacagcat cagatactaa tttttcctct tcagtttttg tcaaaatacc
     9121 tgatttttta aagaatagtg tcaaaaataa aggaataaag atagccactg ccatacagat
     9181 aaagaaatat cccatatatt taacattaat tgacaggaag cctggaagac cgcctacacc
     9241 aatagagtta gcttggacat taaaagttgt cgccaaaaga cctgccacac ttgaaccaat
     9301 cataccagca acaaatggat aaatgtattt aacgtttaca ccaaaaagcg ccggctctgt
     9361 aacaccaagg taggctgaga tagcagctgg aagtgatatt tgagcctctt tttcatcatg
     9421 acgatgcata aaataataag ctagtacagc tgaaccctga gcaatatttg aaagagcaat
     9481 catcggccaa aggccagttg tatgtgtttt tgtatctgct atgagttgag tatcgatagc
     9541 atttgtcatg tggtgcaaac cagtaataac aaatggggca tacagggcac caaaaatagc
     9601 accaaagagc cattttacag gaccagtcaa tccaataaga acgatagcag aaatccattt
     9661 acctagtgtc caaccaatag atccaaggac agtatgagcc aagataatag ctggaactaa
     9721 ggatagaaat ggcacaaaaa tcattgaaac aacttcagga atatgttttc tccagaagat
     9781 ttctaagtaa gacaggctca aaccagctaa cagagctgga ataacttgag cttgataacc
     9841 aattttttgt actgtaaagt aaccaaaatt ccaagaccaa tttttagcaa tgtctgctgc
     9901 tgaagtacta gctacagaat aagcattaag taattgtggc gacactagac agatacctag
     9961 aacaataccg aggatttgag tagtccccat cttacgagtc acagaccata caataccaac
    10021 tggtaagaaa tgaaatatag cttcacctgg taaccataaa aaagagtcta ctccgctcca
    10081 aaacgttgaa acatctacta gagtattcca aattggatgc ccagaagagt ctacttgcct
    10141 gacaccatca acaacttttt gacctaaaaa ttcaaatgga acggcatcta aaatattacg
    10201 aaaaccaagg atcagaccac caacaataat ggctggaata atcggtgtaa atatttcagc
    10261 cagcattgtc aacacacgct gtaaagggtt ttgatttttt tgggcagccg attttgccgc
    10321 ctctttagac acaccctcaa taccagaaac ggctacaaaa gcattataaa aaatagggac
    10381 atcattacca ataatgactt gaaattgccc agcattggta aaggtaccct tgactgatgg
    10441 taactcctca attaccttta ccttagcttt actactgtca ttcaaaacaa atctcatgcg
    10501 tgtggcacaa tgtgtcactg cactaatatt ttctttaccc ccaattgcct ctaacaaggc
    10561 tttggcatca tgtttaaatt gttccatctg tttatcctta tctatttaac ggagagtctg
    10621 tgactccaag gcagaaactt aaagaagact agcttacaat ataattgtaa acgattacat
    10681 atccctatgc aaatccttag cttatctttt taaaaaatct ataaattata aaataattag
    10741 tcaataatac ccaaaacatt agaatttctc accaacgtta ccaaaatgtt agagtcatga
    10801 gtaggtcagt cacccccaat actctcttaa taaaaaacac ctactaattg atatagtagg
    10861 tgagtttttt aatctccatt gaaagatacg atacgattct cttcaatcat atagcattgg
    10921 ttacaaagtt tctctaacat agcgaaatca tgactaataa cgatgagacc taaatttcta
    10981 tcctttacaa tctccaacag gcttttccat acactagctt gtgtaatact atccaacata
    11041 gtagtcattt catctgcaat aaggtattta gtctctggat gtaatgaacg cacaatcgaa
    11101 aagcgttgta attcccctcc cgagagttca ctagggcgac gatttagcca tttttcttga
    11161 attccaaaag catctagtaa atctcgactt gggtaataag cttcttctaa actttttttc
    11221 ataggccata aaggattcat cgttttttca ggatgttgtt gaataagttg tacaggtcta
    11281 aatgctttgt taggcaaatt actgccatca actaatactt caccagatct aggatgtaaa
    11341 aaacctgcaa gcactttgga taaactagtt tttccacaac cactttgtcc aaatatacct
    11401 aaaacttgac caggtgctac ctctaaatta atctccttaa aaagccattg atctttttta
    11461 tgataaaagc caagcttttt agcctctaag gtcatgagta actcctttca aaaattcttg
    11521 ctgtgggaga gagcgccata aacttctagc aaattctgtt tgtaactgct ctccatttcc
    11581 gctaaagaaa ctagctggag ctgtttcaat agcttttccc tctttaaaaa tagtaatacg
    11641 atcagcaatt tgactagctg ctacaatatc atgagtgata aatataacgc ttattccttt
    11701 atctgcaaag gagcgtagtt ggtctaaaac catttgcaga gcatctggat gtaatccagg
    11761 ggtgggctca tccgcaataa tcaaagaaac cttatcacta atacacgttg taaacaaaac
    11821 acgtcggagc attccgccag aaagttggaa aggatacaag tcaccatcac tttcttttaa
    11881 accaaactgt tgaaacaatc cttcttgagt agcctttgaa ttttctgaga tacctaagcg
    11941 cacctgatgt ttgactttca tagatggatc taaataatta actgattgtg gaatcaacgt
    12001 aatatctttt cctcgcaact gtttaatgcg tttagaattt agtgattgac cacgataaat
    12061 catatctcct gttacagatg catttttagg aagaatatcc ataatagcat gtgctaataa
    12121 actcttcccc gaaccactag cacctataat agctaataac tcaccttttt taatttctaa
    12181 attcagcgct tgtatcggtg ttgattgaaa tggttttaaa aatcttccgt attgagtgaa
    12241 ggtgatggag aggtctttaa tgcttaataa tgtttctgtc atatctctac ctcctaaaaa
    12301 tgatcagttt gagggtaaaa gagtttcttt aaagattctc cgatagtatc aaatgcattg
    12361 acaaccaaaa taagataaag tcctggaaag ataaccaacc accaatttcc aagagagata
    12421 tgcttagctg cctctgacag aatgatacca accgaaggtt gttcggcaga gagcccaaat
    12481 cctaagaaag tcattgatgc ttcatgtagg atgacatgtg gaaataagag gataaaacca
    12541 atgaaaattt gagaagcaat caaaggcagg atatgatgcc tcacaatata ataaggcgtt
    12601 tttcccatac ttttagaaag ttggacaaat tctttattct ttagatgata gacttcattg
    12661 cggataagcc ttgctaaaga aggccaatgt gtaacagccg ttgcaatgat gaccccttga
    12721 gcacctttcc caacaacaaa agaaatgaga atcataaaaa tcaaatgagg cataccaata
    12781 aacaaatcaa ctaaccatgc tattatttta tcaataatgc tatttcctaa acctgcaagc
    12841 actccaaaaa ctgtcgccag aatgaccccc ataagggcac ctaataagcc gacttgtaga
    12901 gagaaataaa gtcctttaat cgttctgaca aacatatccc tacctaaacc atctgtccca
    12961 aaaagatggt ttaatgaagg agcaaggttc cgtaaagctg cattggtttg caagggagta
    13021 ctatggaaat aaagatttaa ggctagaata ctaagaatga gagaaatggc gatacccagt
    13081 tgccataaaa caatagttcg acgtctcaat atcatacttt tctccttaac tgtggattga
    13141 ttatgctatt taaaatatcc gcaataagat tgcccgcaaa aacaaataat gtccctatca
    13201 tcacaatagc tagaagtagc ggtgtatcac ttttaagtcc tgcttcagtt agggtagacc
    13261 ctagtcctgg atatgagaaa acttgctcag caagaacgga tcctccaaac aattctccaa
    13321 aataggaaaa atgcagtgta atagctggta cgatagcatt tctaagacaa tgatttttaa
    13381 aaatttgcca ttccgtttcc ccacgcgctc tggcaaataa gacatattca ctagaaagta
    13441 ccgacatcat tttagttcta gtatgaagag ttacattggc aatgcctaga atacttagcg
    13501 tgaaaacagg taaaataagg tgcttaatac gatcagctaa cgtaatatct tgactcaaag
    13561 tacctatcgg agaagaaata ccaatcggga accaccccag ctggacagaa aagattaata
    13621 aaaaaatgag cccaatccaa aaagttggta ctgatatctg aaggtaagaa aaccacctta
    13681 caattcgatc aagcaatttc ccttgatgga aagctgataa agttcctaag ataaatccaa
    13741 taagacccga taagacccag gagagtccca taagtatgaa agaggcacct gcccgtgatc
    13801 taataatatc aataacaggt tgccgataaa ctagtgaagt ccctaaatgt ccctgtataa
    13861 aattttttaa ccaaataaaa tactggaccg gagctggctt atccaagcca tagtggtgag
    13921 caatcgcttt gtactgaaca ggggttagtg acgtgtcata attgacactt gccatgactg
    13981 gatcaaccgg tgattgtttt agtaaaataa aggtcaaaat tgatactcca aaaacgagtg
    14041 acaaacacct aagtatcttc cagataacta cagtggtaat atgtttcaca gataaatctc
    14101 cttaattgtt aaaaaaggag tcgaggaaac cattcacacc ataaggtgta cattgttttc
    14161 tctgactcca aacttaaagt agcctacatt aactaaatta gttacttagc agattcatcc
    14221 caagtccatt cagcaatgtt agtcaataat gaccaatcat gaccatgact atggacacct
    14281 tgtttaccta cattgatacg tttatcacca atataagtat ggttaagact caccaaccat
    14341 acatttggta aatctccaag agtggaagca cctgttttgc catcccactg agctaacttc
    14401 caatatttgt tagctttatc aaggtcggga gatgtcattg ctttgtcaag gtacttagtc
    14461 acagtaggat tgttataaaa agtaatattg gtccaacctt taccagctaa acttgggtaa
    14521 tgtgattcat aaaattgctg cgcatgatga cgtcctcccg cataaagtaa ggctgaatca
    14581 tgtgacttag ttgccatttc atcccagtta ctagccttga gtttaatagt aatacctaag
    14641 gctttagctt gctccgctac ttcaacggct aagtttgctc gtaattgatc attagtaggg
    14701 tagtaaaggt caaattccgc tttaagattt ccttttttgc ggctaccgtc tgcttgttct
    14761 ttccatccag cttttgtcaa aagttgctta gctttagcta ctttattatc tttaattgct
    14821 gtttttggat tccagaatgg tgttctatca ataattgaat aagctggttt accataacca
    14881 tttaaaacag tatccagaac tttttgtcta tttaaaccaa tagtcaaggc ttttctgatt
    14941 gctggatcac tagtgacatc atttcctact ggataaccgt ctggtgaatt tttcacaaca
    15001 cctttcttca cataaggtag cgataaccca cgaacatcat ttgaagcaat atctagaaga
    15061 cgtgttcctt tgactttctt gctagcaagc tctggcgttg cgtaaatcat gtcaacatca
    15121 ccagattcta aagcagctag tgctgtattt tcatcaagta atacccaagt ccacttttta
    15181 aaataaggct ttttaccgtg ccaatatgga tttcgaacga agattgcttg ttctccagcc
    15241 ttatattctt ttaccatgta aggtcctgaa ccgataggat tgctcttata cttatcattg
    15301 taatgttttt tagggacgat tgggatttct gtcaactgtg ctgtaaatgt cgaatgcgcc
    15361 tcagtcaaat gaatatttac ctggtttttc ccaactactt caacattctt aataaaggtc
    15421 aaatcccaag cttttccatc tgctttcaac atatcataag taaacttaac atcgtcagca
    15481 gtaacaggct caccattgga gaatttaaaa tcatcattta agtcgaacga ccacgttaag
    15541 ccatccttag agattttata ttttttagca agctctcctt ttatatctag ttcaggagaa
    15601 cgtttcaata gggtactatg agtaatatta ccttcattat ggattccata acggtccttt
    15661 ggatcgaatt catgaggaag ctttgccccc atagagacaa ccaattcatc tttgggtcgt
    15721 tgttttcgtg ttcgctcctt tgtttgtgaa ttttgatttt gacaagctac taaaaagata
    15781 ctcgccagaa acaaagcaac aaaag
//
LOCUS       DATROY010000038        15132 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.038, whole genome shotgun sequence.
ACCESSION   DATROY010000038 DATROY010000000
VERSION     DATROY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15132
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(121..1425)
                     /locus_tag="VBD11_000952"
     CDS             complement(121..1425)
                     /locus_tag="VBD11_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6953914.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(1572..2471)
                     /locus_tag="VBD11_000953"
     CDS             complement(1572..2471)
                     /locus_tag="VBD11_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6953915.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(2664..4211)
                     /gene="purH"
                     /locus_tag="VBD11_000954"
     CDS             complement(2664..4211)
                     /gene="purH"
                     /locus_tag="VBD11_000954"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6953916.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(4231..4983)
                     /locus_tag="VBD11_000955"
     CDS             complement(4231..4983)
                     /locus_tag="VBD11_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6953917.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(5006..5554)
                     /gene="purN"
                     /locus_tag="VBD11_000956"
     CDS             complement(5006..5554)
                     /gene="purN"
                     /locus_tag="VBD11_000956"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6953918.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(5721..6743)
                     /locus_tag="VBD11_000957"
     CDS             complement(5721..6743)
                     /locus_tag="VBD11_000957"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6953919.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(6771..8225)
                     /locus_tag="VBD11_000958"
     CDS             complement(6771..8225)
                     /locus_tag="VBD11_000958"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6953920.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(8459..12184)
                     /locus_tag="VBD11_000959"
     CDS             complement(8459..12184)
                     /locus_tag="VBD11_000959"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6953921.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(12307..13011)
                     /locus_tag="VBD11_000960"
     CDS             complement(12307..13011)
                     /locus_tag="VBD11_000960"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6953922.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(13135..13374)
                     /locus_tag="VBD11_000961"
     CDS             complement(13135..13374)
                     /locus_tag="VBD11_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6953923.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(13385..14377)
                     /gene="plsX"
                     /locus_tag="VBD11_000962"
     CDS             complement(13385..14377)
                     /gene="plsX"
                     /locus_tag="VBD11_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6953924.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(14455..>15132)
                     /locus_tag="VBD11_000963"
     CDS             complement(14455..>15132)
                     /locus_tag="VBD11_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6953925.1"
                     /translation="PILASILLFLIYFQKRDFTSLGFTKDNNVLTTISGFITGSLLMF
                     FIYICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYVMTTVTKFKGSF
                     AGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVTGVHTAWNFT
                     MGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSLILVGCLAYFSLER
                     VKVSVRY"
BASE COUNT         4500 a         3401 c         2728 g         4503 t
ORIGIN      
        1 aacgtgaaag tagtttcaga cttatagctt aatctcctca atttaataaa aattatataa
       61 gaaccttgaa agtctattca aggcatataa aaaagaagcc aaatagcttc cttttttata
      121 ttatttgtta aatgatacgt gaacgtggtc atagtggttg gcagtaacgc caccacgatc
      181 tggcattgca ttccaagtat tagcaggtcc ataaatacta tttgtatttg aataaaactt
      241 ttgttgccag ataacatatg aaatgttatt tgctgccata ttttgtgtag agtactgtgc
      301 aacttcatta ccaagtgctt ggtttttacc tacaataaag tcaactgcta aacctttacc
      361 atgatcacct ggatctcccg cacggtatgt actgaattca ttaactccat aagttgacgc
      421 tactttttct ttataagctg caacatgagg ttggagccct gcattttcag gatgtgcagc
      481 tactgcattt gttgttgaag ctggttgtgc taccggtgtt gctgttggag ctttttgtgc
      541 taccggaacg ctcttaactt cagtcgcttg taacttactg tctgtagctg gtgaagtcgt
      601 agtcacagga actgctggag ctgatacatg ctctggtgat gcaccagttt ctactttagg
      661 agtgactact ttagcacttg ccactctagg ggctgctaca gttcttaccg gtgctacttt
      721 agctactgga gctggtgttt cagcggcaac agaagctggt gatactgttg ttaactgact
      781 gactgacgtc tgagttggtt taacttcctc tttagctgct ggaacttctg aagtaatcga
      841 cttcacagga gctggtgata cctgttcatt agctgctact tgactaacag cttgctcttg
      901 tgctaatact tcttttgatt tcaaagctgg cgcagaagaa tatgtcttca ttggcgaaac
      961 aatcgttgtt gctgcttctg gtgtcatacc ttccgaaatt gtattgagag aaactttttg
     1021 gtctgcaaca aaaacttgat tggttttcaa atcgacagta gctgttgttt gaccagcagc
     1081 atttgttgct ggtgtttcta ttttcattga agtggcagta tgactcttct gatcgtaagt
     1141 tactgtcagt gttgtctcag gataaataag attgatatct gcaatgttat taatttttgc
     1201 taagacattc atatcaattg acattgcttc tgaaataacg cttagtgtat caccatattt
     1261 cacagtatat gatgatttat tgtcttgctt taccaaatca gcctttacct ctgaaacagt
     1321 acgtgctgtc cacgtcgtat ctgtttcttg tgcttgaaca cttgcgactg ataatagcga
     1381 agctgccatt gtcgatgtca atagtacctt tttattcatt ttcattgctt caattcctat
     1441 ttccttttta ttagtccgag actataatat cacactttac tggactataa aattattttc
     1501 agacggttta aagaaattgt acttagttag taacaaaact gtcatttaaa acaatgaatc
     1561 aatacctcaa actatttgta aagcagttct tgactgttgt caacgcgaag ccaaactgaa
     1621 ctaccattag caaagcgtgt cttagcccaa gttaaaccag ctgttccttg gatgggtttt
     1681 tctacagtct taagagtttt aggattaaag ataaagtaac cacctttttg aagaacctgg
     1741 tcagctgtca aattaccatt agcatcaacc tcatcaattt ctgatgctgg tataccatta
     1801 tcgttccaat caaacccagt cggcgttagg gtgttatttt tgactaacca aacaccatta
     1861 accttttgta attcatctac tcgatacact tttaatttac tagattgatt ctgtacagga
     1921 gctgattgta atggcttaat gcttggagct gatgcttgcg tttttccact aaaggtcgca
     1981 acgttagcaa ttagtgacgt tggattgata cgtccatgga aaccattttg aaaattaggg
     2041 ttagctggta aaaattcaaa atgaaggtga ggtcccgtcg ccataccagt tgctcctacg
     2101 taaccgatga tatctccttg tttgactttt tccccagtcc tagccaccac acgtgacata
     2161 tgagcgtaac cactatgcat tccatccgca tgttgaatca tgacacaatt tcctgctaag
     2221 tctgtcatcc aagaaaagtt ggctccagct cctgcaaatt tcacagtacc atctgccact
     2281 gccctaataa tcgttccagt cggaacagca taatccaccc cacaatgtcc aggataacca
     2341 ttgaaacctg ttgtaattct accattatca attggacgga cataagtatc cgctaaaact
     2401 cgggaacccg cagataaaac cataccacct aaaacaacta aggaacttgc cttaactaac
     2461 catttattca agaaaaacct ccaaatagaa ttaatataat gaagtgctca aacacttgca
     2521 attaacttat tcgaaaattt aaatcaaaat atcaaaaaaa gtactcacct tgatgagtac
     2581 ttaggaggtt taataaaaag aaaagttttt aggatatacg tagtatacct gagttaactt
     2641 aagaaaacct aaactactat tttttaatgt ctaaaatgtc tcacgcctgt gaagatcatg
     2701 gtcaagccat gtttgtttgc ggcgtcaata gattcttgat cacgaactga accacctggc
     2761 tggatgattg ctttgatgcc tgcggcagct atttcttcaa tgttgtccgc aaatgggaag
     2821 aaggcatctg atgctagaac ggcaccgtca aggtggtcct tagcctgctc gatagcaatc
     2881 ttgactgagc cgacacggtt ggtttgacct acacccagtc caagcgtcat gtgatcgtta
     2941 gtaataataa tcccgttaga cttaacatac ttgatagcct tccaggcaaa ctcaagggca
     3001 gtcacctctt gttctgttgg ctggcggtct gtcaccactt gccagtcaga tggattttca
     3061 gccacaacgt cttggttttg caccaaaagt ccaccaacta cgccagtgta ctcagcttcc
     3121 acttcgctgg cagcttgggc atcaaacggc aactcaagga tacgcaagtt tttcttttta
     3181 tttgtgagaa tagctagcgc ttcttctgag tatgatggtg cgatgatgat ttccaagaag
     3241 atagggtgca tcttctcagc tgtcgctgcg tcaacttcac ggttaaggac aacaattcca
     3301 ccaaagattg aaactggatc agcttcataa gcgtaatccc aagctgtctc aatatcatca
     3361 gcctgtccga taccacatgg gttcatgtgt ttgagggcaa caaccgttgg actgtctttg
     3421 aaatcgcgga taatacggat tgctgcatca gcatcacgga tattattgaa ggacaattct
     3481 tttccattga gctgtttagc tgaagcgatt gagtagtctg ttggtaaggc tttttggtag
     3541 aaatcagcgt cttgctgtgg attttctccg taacgcatag cctgtttaag atcataagtg
     3601 atggtcaatt tttcaggctt agcctctccc acttgagctg tgaagtactc agcaatcaaa
     3661 gcgtcgtagg ctgccgtatg acggaaggcc ttagctgcca agcgttgacg agttttaaat
     3721 gtcgtctgac tagcgtcagc caattctccc aaaacagtgg catagtcagc tggatcaacc
     3781 acaacggtta cgctagcgtg gtttttagca gctgagcgaa gcattgatgg accgccgatg
     3841 tcgatgtttt ccaccgccaa atcgttggtc acgtctgggc gaaggatagt ctccttgaag
     3901 ggatagaggt tgacaaccac gaggtcaatc aactcgatgt tgttgtcctt agcagcctga
     3961 aggtggctgt cagcgtcgcg acgagccaaa atcccaccgt gaatgtttga gtggagggtc
     4021 ttaacacgac catccatcat ttctggggag ccagttacat cgtcaatagc aatggtctca
     4081 acaccagcat cgtcaagggc aaccttagtc ccaccagttg agataatatc ccaacccaag
     4141 tttttcaatt cttttgcaaa gtcaataatt cctgatttgt cagaaactga gattaaagca
     4201 cgtttagtca tgagttctcc ttttcttact tcaatctatt tcataggcta tgtattcatg
     4261 agaaaattca taaccgagtt tttcagataa attcaatgaa gtccttgtat gagcatccca
     4321 gctaggataa attcccttat ctaaacaagt tagtatcaac tgagctgcaa ctattgttgc
     4381 taaaccacgc cgacgaaaat ctggatgcgt atctacttct atctcgatcc catttttata
     4441 ggttgaataa gatgaagctc ctgcaatgat attcccctga taatatataa cataacctat
     4501 accttgtttt ttatagtact gataagtagc gtaatttgct actaaatcct gtgaccattc
     4561 cttttctaag caagagttgt aaactttctc atcaataaca cgtaattcaa aaccatttgg
     4621 caactgagta acaaacttct ctaacctact tcgctcaaac aaagtatctt ttttcgtcgc
     4681 ataacgctta aaagaatggg cattctggcc ataagttgat tcaatcaaat ctgaccatcc
     4741 tttatgctga ggaacaagga taatatcctc cccagagcaa acttctagta gaaataaagt
     4801 aggctgacct gctagaaagc caaaagatga tttccttccc aattttgcca aagaagattt
     4861 tggttggtct aaactatcta caaaaacttc tcccataata ccttggacac acgaccagat
     4921 tatggtctcg tcccaatcac caaaaataga ctctactatc tctaaatttt tcactagttt
     4981 cattagctta ttataacaaa ttttactatt ttctctttat gcccaaacta tccaaaacat
     5041 ctggatagag ttgatattcg gtttcatgga tgcgggtttc aaagctttct aggctatcgt
     5101 ctgctaggcg tggcacgcgc acttgttgga tgacctgacc ggtatccaca ccagagtcca
     5161 cccaatggat ggtcacacca gactggtcaa cacctgcctc ccaagcatcc ttgataccgt
     5221 gagcacctgg aaattcaggc agataggctg ggtgaatatt gatgatacgc ccttcatagg
     5281 ctgagagcaa ggtttctccg acaatcttca tgtagcccgc cagacagacc aagtcaatct
     5341 cgtgtttgtc caacaaatca acgacggctt gctcgtaagc cgccttattc tcaaactctt
     5401 tgagttcaaa agcgaagctt ggaatggcta agttctgagc acgctctaaa acataggcat
     5461 cacgatgatc tgaaaagaca aaactaactg gaaactgctc tgctatgacc tgaaagttgg
     5521 aaccattagc agaagcaaaa acagcgattt tcatattcga tacaaaggga tgattcggct
     5581 tgccgaaaaa tttctagaaa aataggaaaa cgaacaacga cgcttgcgtc taagtcgttt
     5641 tatctttttt cccagaaatt taatcccgtg ttcagttagg caaactaact aaacactcct
     5701 ttcttataaa atttagtgac ttatttaatc accacactat cgtctgcttt cttgatgata
     5761 cgaccgattt cataaactgg ttcgtccaag agctctttga cgcggttaac attttcagga
     5821 ctaacagcta gcataagacc gacacccata ttgaagattt caaacatttc ttcgtgcttg
     5881 atgtcaccat atttttcaag cgccttgaaa atcggaagta ctggcacctt atcctcatcg
     5941 atttccgcag ccaaatcatc cgcaaacata cgaggaacat tctcgataaa gccaccaccc
     6001 gtgatgtggc cgataccgtt gactagttct tccttgatta atggcagagc tgcttgaaca
     6061 tagatacgag ttggctcaag aaggacatcc ttgagttgtt taccttcaag ctctggaagc
     6121 agctctttac cagtatagtc agcaaagaca cgacgcacca gcgaataacc gtttgagtgg
     6181 ataccacttg aagcaagtcc caaaagaata tcgccttcct ttactttgga accatcaatg
     6241 agctgagatt tttcagccac accaacagca aagccagcaa ggtcataatc atcttcgcca
     6301 tacatcccag gcatttcagc ggtctcacct ccaatgagtg ctgctcctgc ttgaacacaa
     6361 ccttctgcaa cgccagaaac aacttcttca agtttgactg ggttattttt tcctgtcgca
     6421 atataatcta aaaaatataa tggctcagca cctgctgcaa taatatcatt gacacacatg
     6481 gcaacacaat cttggcctat ggtgtcatgc tgttgatatt taatcgcaag catgagtttt
     6541 gtgccaacac cgtctgttcc tgaaaccaaa ataggctctc taactcctgt tttactcaag
     6601 tcaaacaccc caccaaagcc acctagagct cccatgactc ccgcacgttc tgtacgagaa
     6661 acgtgttttt ttattctttc aaccacttca taaccagctt caacatcaac accagatttt
     6721 gcataagcat ttttttcaga catgttttct ccttatataa ttagcagagc ttatttcact
     6781 ttttgaatat aaaaacttgt ttcttcttct aaacttctga ggtattcttc ttcataatca
     6841 taaagcggtg ttggataatg tccatcaaag taagctacgc ataaaccacc atttggtgct
     6901 tttgtttcga gtccaattga ttcaatcaac ccatctagtg agagataggt cagactatct
     6961 gcgccaataa tatcacacac ttcgtcagca gcgtgatttg ctgaaattaa ttctcgtctt
     7021 gtttgaatat caataccata aaaacaagga tactttaatt ctggactagc tatagcaaca
     7081 tgtacttcac tagctcctgc ttctcttaat aatccgacaa tccttctaga agtcgttcct
     7141 cttacaattg agtcatcaat cataacaacg cgctttcctt tgacaacacc agataccgct
     7201 gatagtttca ttcgaacacc ttgttccctt aattcttgtg tcggttgaat aaaggttcgc
     7261 tgcgtatact gattttttac aagacccatc tcatttggta atccggattc ttcagcaaag
     7321 cccatagcag ccgataagga cgaatttggg acaccaatta caatatcagc atcctgttta
     7381 aattcttgtg caagacgctt tcccatattt tttcgagccg tatgaacatt aacaccatgt
     7441 atagttgaat ccggccttgc aaaatagaca tattccatcg aacaaattgc cagttgcgtt
     7501 tcatcagtat aacgatcaca ttgaatacca ctatcgtcta taaggataac ttcgccaggt
     7561 tcaacatctc taacccattt tgcgcctacc acctcaaaag cacaggtctc actggaaata
     7621 acccaagcac cattttgcat ttgtccaatt gacaaaggac gaaaggcatt agggtcaaga
     7681 gcagcaatta atttatcttc tgtcatcagt agataggcga agcctccctt tacagtgctt
     7741 aaagcttctt ttaccttccc cataaaactt gggttatggc ttcgacgaat caagtgcatc
     7801 aaaatttcag tatctgagga ggcattgaaa attgcacctt gcttttctaa ttctttcctt
     7861 aaagaaatag catttgtcaa attaccatta tggcataaag caaattgccc gtcatgaaat
     7921 ttataaagaa aaggctgaat attgcgaata tctgcagaac ctgcagtagc ataccgaaca
     7981 tgtccaatag ccgcattccc agttaaatta tctaattcag attgattctt aaaaacttca
     8041 gaaaggagcc caacatttcg ataaccatag agtttcccat tatcattcga aacaatacca
     8101 gcaccttctt gaccgcgatg ttgaaggcta tgaagcccaa agtaagtgac ttgagctgcc
     8161 tgaggatgcc cccagatacc aaagactcca cattcttcat ttagagattt tacttcgtat
     8221 gtcattcttt tttcctaatt ctttttgtat ctcatcttaa gtagctttga tgattttctc
     8281 aattttggat ctatcgtcac caatgccaac atcaaaatag aaaatctatc aagctatttt
     8341 caagctaatc attgagtctt agcctcaacg gctcccaaat accactacga cagaaagata
     8401 cagttaagct cgcttcgctc ttgtttcctt atctgtttta tgaaaaaatt tgaaaaaatt
     8461 attttcctgt aaaatatttt actgcactag caaacaaggc ttggtctttg ttaccaggga
     8521 tattttggaa gaggccgtct tcccagcgtt ctgagtgccc catcttaccg atgatttgac
     8581 cattcttgct ggtaatccct tcgatggcat tgacagagcc atttggattg tatttagaat
     8641 ccatagatgg ttgtccgtca aagtccacat attggctcca gatttgacca ttgtctctta
     8701 gctctgcaaa ttcagaagcg ctgacaacaa atttaccttc accgtgtgaa actgggatgg
     8761 catgaatatc gccgacctca actcctacca accaaggtga gttggtattt gcgatacgag
     8821 tctcaaccat cttggcaacg tgctggttgg catcgttata gaagagggtt ggacttgtct
     8881 caccagcttc ctcgaagttt ccgtatggaa gaagacctga tttaacaagg gcttggaaac
     8941 cattacagat accgatgata aggccacctt tttcgatgaa gctgtcaata gctgcgcgaa
     9001 ccttcttgtt aagcaagata ttgacgataa acttagcaga cccatctggt tcatccgctg
     9061 ctgagaatcc tccagcaaag aagatgatat ttgccttagc aatattagcg accattgtgt
     9121 caactgagtc agcaatagca gcctcattca aggttacaaa tggtaccaag ttgacactag
     9181 ctccaacctg ttcaaaggcc ttagctgaat catattctga gttggtacca gggaagactg
     9241 gaatgtaaac cactggtttt tcaattgttt ccttagcctt gataacagta tctgatacca
     9301 cagcaggaac ttcttcaaga gcgtctgcct gttcaaactc tgttgggtaa acctcttcca
     9361 atttgccttc gaagactgat agaaggcttg ccccagcaag gtcatttcca ttgacagtaa
     9421 gtgtaaaggc tgcctgagtt tgaccgattt tcaccgagtc agcaatttcc tcagctgatg
     9481 taaagacaaa gcctccgagt tgagctgtca agctactgtc aagctctgca atttcaacag
     9541 aggccccgat acggttacca aaagtcatga gagcaagact ttctaggaca ccaccgtatt
     9601 taacagctga agcagcagta atcttatgtt gagcttgaat agtctcgaac tggctaaagt
     9661 tagccttgat aaggtcaaaa tcaatatctt ctgaaatagc ttgacctgga atgtagtaga
     9721 tattctcacc agccgcttta aactcaggag agagaacctt gcggctgtcc gctgttgtca
     9781 caccgaaagc taccaaggtt ggcggtactg tcaattcttc gaaagtacca gacatagagt
     9841 ccttaccgcc gattgatggc aatccaagtt gaatctgagc ctcaatagaa ccaagaagag
     9901 ctgatactgg ctgatcaaaa cgctctgcct gtttatccat acgctcgaaa tactcttggt
     9961 aagagaaacg tgcacgagac cagtcagcac ccgttgctac caagcgagct gtcgcttcaa
    10021 tgacagcata ggcagcaccg tggtaaggtg accattctgc aatataagga ttgtaacctt
    10081 gagccataac agatgcagtt gttgtgacac cgtgttgaac tggcaatttt tgaacagagc
    10141 tttctgttgg tgtgatttgg taacggccac cgattgggtg gttaacggtt gagcgaccaa
    10201 cagatgagtc aaagatagtt tggagacctt tttgactagc gtggttgagg tcagacaaga
    10261 ccttaagcgt atccgcttca agtgtctctg ctgatgttgt gcgtacctct ggaactgtca
    10321 agtctttgtc aacgacttta gcatcaacaa cgacacggac accgttggta tcaaggaaac
    10381 ggcgttccaa gtcaacaatt gtttcaccat cccaagtcat gacaaggttt ggttttgcag
    10441 taatagtagc aaccacaacg gcatcgatat tttccttgtt gcaggctgcg atgaaggtat
    10501 ccacgtcact aggacgaaca acgactgaca tacgctcttg tgattctgag attgcaattt
    10561 cagtaccatt aagaccttgg tatttaagcg gtaccttgtc caaatcgatt tcaagaccgt
    10621 cagccaattc accgatggca acacagacac cacctgcacc gaagtcattt gatttcttga
    10681 ttaggcgagt gacattacca tcacggaaaa ggcgttgaat cttacgttct tcgatggcat
    10741 tccctttttg tacctctgcg ccagctgttt ccacagattc aaccgtttga accttagatg
    10801 aacctgtcgc accgccgaca ccgtcacgac ctgttttacc accgagcaag atgaccacat
    10861 cgcctgcttc tggtttttca cgaaccacat tttccttagg tgcagcacca accacagctc
    10921 caagctccat gcgtttggct acaaagccag ggtgaaagta ctcacgcaca taagttgtcg
    10981 caagcccaat ctggttacca tatgaagaat agccgtgcgc cgcagtttta gaaataacct
    11041 gttgcggcaa tttaccagca cgtgtttccg caatcggagt cgtaatatcg cctgcgcctg
    11101 aaatacgcat agcctgataa acgtatgaac gtcctgacaa cgggtcacga atggcaccac
    11161 cgatacaagt tgctgcgcca ccaaatggct caatttctgt tgggtgattg tgagtctcat
    11221 tcttaaacat gaggagccaa ggctctttta caccatcaac atctacttca atttcaactg
    11281 agcaggcatt gatttcatct gagacttcca tatcatccag tcgtccattg gcacgttcat
    11341 aacgaccaaa gatagtcgcc atatccataa gtgtctgcgg cttttcagaa cgaccaagtt
    11401 catcacgcat ggcgatatat ttgtcataag tcgcctgcaa ttgtttttgg aatttagaag
    11461 ctgaaaagtc gatgtttttc aactcagttt caaaggttgt gtgacggcag tggtctgacc
    11521 agtaagtatc caaaactttc aactcagttt cagttggcac acgcccgatt gatttgaaat
    11581 aatcttggat gaagagaaga tcatcgacct ccatagccaa gccctgctct gccttatagg
    11641 ccgcaaaatc gtcagcctta taagtttcaa agaaatcaag actagggatt gtcttatcag
    11701 acacagagaa agcctgctct tcaagcggca aggtaatgtc cttgaaacgt gaatcaacag
    11761 gattcaaaag atagttctta acggcttcaa gctctgcttc tgcaatatcc ttattgacca
    11821 agtaaagctg ggctgtattg accttaacct gactgtcact tccaagcaat agcaaagctt
    11881 cttgcgaact agcagcacgt tggtcaaatt gaccaggaag cgcctcaatg gcaaagaagg
    11941 caaccttatc aagctccgca gtgatttcag tttccgtcaa aagacagtct gtcacctgct
    12001 cagagaaaat atgcttctca gcacgcgcca gcaaatcctc agccaaattg aagacatcat
    12061 agacctgcac aatacgcaaa tccttcaaag aagtcagttg tagattatgc gtcaactctt
    12121 tcacaagact agccgattta atgtcaaagt cagccttttt ctcaacaaaa atacgtttat
    12181 tcatataaga taccaagtcc gacaagggag cgactgaaaa ataggaggat atctgaaatt
    12241 gagatttcag taagcccaat cttttttctg agtttcccga acggattcaa ttccttcctc
    12301 ttctaattac aatgctatca acttctcaag tacaacttga tagacatcgg tcaagctacc
    12361 aagatctcta cgaaaaacat ccttgtccat atgatttcct tcagcatccc atagacggca
    12421 gttgtcaggt gagaactcat cagccaagat gattttgcca tccttgtcaa atccgaactc
    12481 caacttgaag tcaatcaagt taagcccaat ctgagcaaac caatctttca agagttcatt
    12541 aatacggcga gtctctcctt tcagataagc aatttcttca tcattaacaa tacccaaaaa
    12601 cttcatatgc tcatcattga taaaaggatc attcagatca tcatttttgt agtaaaattc
    12661 cacaattgga gtttcaagaa cgcgtccttc ttccactcca aaacgttttg aaaatgagcc
    12721 tgctgtcaca ttccgtaata ccacctcaag tggaataata tcaacttttt tattcaattg
    12781 ttcgtttttt gaaatttgct caatataatg tgtcaccaca ccagccatat ttaatttctc
    12841 aaaaataaga gatgaaatct gattatttag tgctcccttg ccatcaatag tctccttacg
    12901 agctccattc aacatagtag cttggtcttt atagaccgtt ctaatcacat tttcatcttt
    12961 tgtactataa atatctttcg cttttccttt ataaataagc tggttagtca cttgattatt
    13021 cgcctttctc ttattttaag agttattcta acacacaaca ttatctaata caacaaaaac
    13081 tttatttaaa atcaaaaaaa agaagaaacg ttcgtatttt cttccttttt attattactt
    13141 atgttcaatg atataatcta atagttgttg gacgcttgac atgtgttcga cagcctcatc
    13201 tggaatctct agatgaaaca catcttccaa attgataata aactctgtta aagcgataga
    13261 gtcaattgct aaatcatcat gtaacgatga ctctggtgtg atatctaact gtgggtcacc
    13321 aagttgctgc cttaataatt ccagcatctt ttcaaaaact tcatctcttg acatgagttc
    13381 actcctattc atctgtaaag gcatcaacta attgatcaac tacttgagtt tccaacattg
    13441 tacgaacttg cttcaaagta gaataaacag ctttagaatc acttgatcca tggcatttta
    13501 ctattggagc ttttaaacca aaaagaaccg cacctcccgc gcttgaatag tccatactat
    13561 cttttagctg atacaggcta tcttttaata gaagtgctcc taatttagct ttcacgccac
    13621 ctgatttaat tgaagatttt agacttccca taatattcat agctgtacct tccattgtct
    13681 ttagaacagc atttccagta aaaccatcag tgacaacaat atctgctaca ctagacatca
    13741 aatcacgtgc ttcaatattt ccaataaaat taatagaagg ctctgctgct aacagttcat
    13801 aggcttcttt atgaagagaa tcgccttttg tttcttccgt tccgttattt aagagaccta
    13861 cacgaggaac ttcaatacca cggacatttt ttgcataaaa cgaccctaaa atagcatatt
    13921 gatgaagatg agaagctgtg ttttctgcat tagctcctaa atcaagcata tcaaaaccca
    13981 caccatctaa cgtaggcata gttgacatta agcctggtcg atcaacacct tttatacgtc
    14041 ctactacaaa caaaccagca gctaataaag ctcctgtatt ccctgcagaa ataaaggcct
    14101 gtgcaacacc atctttgact gccttagctc ctaagaccat cgaagactgt tttttacgac
    14161 gaacagcttt agcaggctca tcatcagaat taattttttc ttcagtatga ataatactaa
    14221 ctcgctccgt tactgttaaa tatttctcaa tttttttctg atcgccatag agttgtactt
    14281 caatatcact aaaatctgaa atagcttgat taacaccctc tacaatagct ttgggagcgt
    14341 aatctccacc catagcatct acagcaattt tcttcatgaa tgaactcctc ataaaaactt
    14401 agtggctata ttatagcatg tttctttatt tttttcacaa gaaggcaata atagctaata
    14461 tctaacagat actttcactc tttccaaaga aaagtatgct aaacaaccta ctaatatcaa
    14521 agaacatagt actccccctt caaaaccaaa atccccacca ctccaaatag caaagctaga
    14581 attttcagta attctaaaaa gagaaacagg agaatacccc ccactaacct tattacctaa
    14641 tacacatccc atagtaaaat tccatgcagt atgaactcct gtaacaaaaa gaatattttt
    14701 ggtcatatta aaaagaatag aaaaaataat accaagtaaa aataaattaa aaagtgctat
    14761 tgctgtgata ccatagtttc taaagtggat aaaggaaaac aacatagaat tgcataaaac
    14821 acctgcaaaa ctacctttaa atttggtaac tgttgtcata acatatcctc taaataaaat
    14881 ttcctctgtc aagctttgga taaagtatgc aagtaataaa aagaaaaaag gtccaaaaga
    14941 aaaggaattg ctggaagaaa tttccacaaa acctgataac cagtggcaaa tataaataaa
    15001 aaacatcagt aaactaccag taataaatcc tgagatcgtt gttaaaacat tattatcttt
    15061 agtaaatccc aaggaagtaa aatccctttt ttgaaaatag attaaaaata gtaaaatact
    15121 tgccaaaata gg
//
LOCUS       DATROY010000039        14662 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.039, whole genome shotgun sequence.
ACCESSION   DATROY010000039 DATROY010000000
VERSION     DATROY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14662
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..272
                     /locus_tag="VBD11_000964"
     CDS             <1..272
                     /locus_tag="VBD11_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6953926.1"
                     /translation="YSGKSKTIQYEYHEVFLKEHRVNKLAYCLLAFFLGSIGGQYFYM
                     KEFKKGLLCFFLSWTTVPIFIGFYQAIKAIFEPFNNDDTISIYTK"
     gene            399..1814
                     /locus_tag="VBD11_000965"
     CDS             399..1814
                     /locus_tag="VBD11_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEO6953927.1"
                     /translation="MRKVAIYSRVSTINQAEEGYSITGQIDSLTKYCDAMGWVIYKNY
                     SDAGYSGGKLERPAISELIEDGKNNKFDTVLVYKLDRLSRNVKDTLYLIKDIFTKNNI
                     HFVSIKENIDTSSAMGNLFLTLLSAIAEFEREQIKERMQFGVMNRAKSGKTTAWKTPP
                     YGYTYDKENKVLLLNEFEATNVKQIFNMIVAGHSIMSITNYAKEHFAGNTWTHVKIRR
                     ILENETYKGLVKYREQTFAGNHDAIIDEELFTKAQLALDKRTNSQNNTRPFQGKYMLS
                     HIAKCGYCGAPLKVCTGRPRVDGTRRQTYVCVNKTESGAKRGVNNYNNNKVCNSGRYE
                     KSCVEKYVINELSKIQHDKEYLEKMKNNSKKVDVSSLKKEIKSIDKKINRLNDLYVND
                     FISLSKLTEEIKKLNKLKEGYHKTIKLNYVENKNEDVISTLVNNIDISKSSYDVQSRI
                     VKQLVDRVEVTTDNIDIIFNF"
     gene            complement(1872..2783)
                     /gene="whiA"
                     /locus_tag="VBD11_000966"
     CDS             complement(1872..2783)
                     /gene="whiA"
                     /locus_tag="VBD11_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6953928.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            complement(2780..3754)
                     /locus_tag="VBD11_000967"
     CDS             complement(2780..3754)
                     /locus_tag="VBD11_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6953929.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            complement(3751..4641)
                     /gene="rapZ"
                     /locus_tag="VBD11_000968"
     CDS             complement(3751..4641)
                     /gene="rapZ"
                     /locus_tag="VBD11_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6953930.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            4795..5208
                     /locus_tag="VBD11_000969"
     CDS             4795..5208
                     /locus_tag="VBD11_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6953931.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     regulatory      5266..5310
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5454..6437
                     /locus_tag="VBD11_000970"
     CDS             5454..6437
                     /locus_tag="VBD11_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6953932.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     gene            6434..6991
                     /locus_tag="VBD11_000971"
     CDS             6434..6991
                     /locus_tag="VBD11_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6953933.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(7031..8377)
                     /gene="asnS"
                     /locus_tag="VBD11_000972"
     CDS             complement(7031..8377)
                     /gene="asnS"
                     /locus_tag="VBD11_000972"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6953934.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(8398..9591)
                     /locus_tag="VBD11_000973"
     CDS             complement(8398..9591)
                     /locus_tag="VBD11_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6953935.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            complement(9677..12184)
                     /locus_tag="VBD11_000974"
     CDS             complement(9677..12184)
                     /locus_tag="VBD11_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6953936.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            complement(12292..13056)
                     /locus_tag="VBD11_000975"
     CDS             complement(12292..13056)
                     /locus_tag="VBD11_000975"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6953937.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSLEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            13224..13922
                     /locus_tag="VBD11_000976"
     CDS             13224..13922
                     /locus_tag="VBD11_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6953938.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            13919..14629
                     /locus_tag="VBD11_000977"
     CDS             13919..14629
                     /locus_tag="VBD11_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6953939.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
BASE COUNT         4806 a         2867 c         2355 g         4634 t
ORIGIN      
        1 catattcagg aaaatcaaaa actattcaat atgaatacca tgaagtattt ctaaaagagc
       61 acagagttaa caagctagct tactgtcttc ttgcattctt ccttggcagc atcgggggcc
      121 aatatttcta catgaaggaa ttcaaaaaag gtttgttatg cttcttcttg tcatggacta
      181 ctgttccaat tttcattggt ttttaccaag ctattaaagc tatatttgag ccatttaaca
      241 acgatgacac aataagcata tatactaaat agaacaataa aaaaagcccc acgctcaaat
      301 ttggcgagga gagcgtaagg cgaatctagt atagtaaaaa cctgctttaa gtaggctctt
      361 tactgtacct attttaacaa aaaatgaggt aaaaaacaat gcgaaaagta gcgatttatt
      421 ctagggtatc aacaataaac caagcggagg aaggatattc tatcactgga cagatagact
      481 ctttaacaaa atattgtgat gctatgggat gggttatata taaaaattat tctgacgcag
      541 gatattctgg aggaaaatta gaacgaccag cgatatcgga actaatcgaa gatggaaaaa
      601 acaataaatt cgatactgtt ttagtttata aattggatcg tttatctagg aacgtaaagg
      661 acacacttta cttaataaaa gatatattta cgaaaaataa tatacacttt gtaagcataa
      721 aagaaaatat tgatacttct tctgctatgg ggaatttatt cttaacgtta ctatctgcaa
      781 tcgctgaatt cgagcgtgag caaataaaag agcggatgca attcggcgta atgaatagag
      841 ctaaatctgg gaaaacgact gcgtggaaaa ccccgccata cggttacaca tatgataaag
      901 agaacaaagt gcttttgctt aatgaattcg aagcaacaaa tgttaaacaa atttttaata
      961 tgatagtagc tggtcattcc attatgtcta tcacaaatta tgcaaaggaa cattttgctg
     1021 gaaacacatg gacacatgta aaaatacggc gtatattaga gaatgaaaca tacaaagggt
     1081 tagtgaaata tcgagaacaa acgtttgctg gaaatcatga cgctattatt gatgaagaat
     1141 tatttacaaa agcacaactt gcgctagaca aaaggacgaa ctcccaaaat aacactagac
     1201 catttcaggg taaatacatg ttatctcata tagcaaaatg cggatactgt ggcgctccgt
     1261 tgaaagtttg cacgggtaga cctagagtgg acggaacaag aaggcagaca tatgtttgcg
     1321 ttaacaagac agaaagtggt gctaaaagag gagttaacaa ttataacaac aataaagtat
     1381 gtaattctgg aagatatgaa aagtcgtgcg ttgagaaata tgttattaac gaactctcta
     1441 aaatacagca cgataaagag tatctagaaa aaatgaaaaa caacagcaaa aaagttgatg
     1501 tttcgtctct taaaaaagaa attaaatcta ttgataaaaa aattaatcga ttgaatgatc
     1561 tttacgtaaa cgattttatt agtttatcaa aactaacaga agaaattaaa aaattaaata
     1621 aattgaaaga aggatatcac aaaacaatca aattaaacta cgtagaaaat aaaaacgaag
     1681 acgtaatatc aacattagta aataacattg atattagcaa gtcttcgtat gatgtacaga
     1741 gcagaattgt gaaacaacta gtagatagag tcgaagttac aactgataac atcgatatta
     1801 tttttaattt ttaatgcatt attgacaaac gacaataaat attgttgtac aagccatgct
     1861 aatatctcct tctataattc atctgcaatt ttatttattt tccgtaagcg atggttaacg
     1921 ccactcttac tcagaggcgt ttctaaactg tctgctattt gttgaataga gtaatctgga
     1981 tgagccacac gcacctgagc tacttgtctt aaatcactag gcagtgcatc aaaaccaata
     2041 gtatccataa tcttgataat attattgata gtcttcatac tagctgtgat tgttctagca
     2101 atattagctg tttcaacgtt gttagcacgg ttaatatcat tacgtgtctc acgtatcatt
     2161 ttaatctctt caaaagcatc tctagcttcc atggcatcaa tgactatcaa gaaatccata
     2221 atatcttctg ctttttgcaa ataagttact gctccatgtt tatgttcaat aacctttgca
     2281 tctaacataa attttttcat aagattagct aagtcttgtg catggtctag ataaacagaa
     2341 aaaatttcta gttgatattt tccagaatca ggttctctaa cagtacccgt tgataagaat
     2401 gctcccctga gataagctct tccattttca tcattgtcca atatagagtg ttcaatacca
     2461 gtttcaatac caaaaaaagc atcagccaac ttcaaatcag caagaataac atcaactttt
     2521 tcttcaataa aaacagtata cacacgattc tttcgtaagt tcgttttttg atggtatttg
     2581 atttctggct gcaaatgata atgttcttct aacatgctat aaatatgacg agcaatttta
     2641 gcattttctg tggtaataga aaggttgagg ccgtggttag ctaatcctaa actacctgac
     2701 attttgataa tagctgacag ttccatctta ttttcactct tatgacctag caactcttct
     2761 ttgactttta ctgtaaaact cataaatgtg ttcctactaa attcattagt tcctcaacaa
     2821 cgaagtcacc atgatggaag gctccacctt tttctaattt taaaaagtta gacgagatga
     2881 cacgtctggc atgttttcta agccctgaaa aatcatgttc aacctgtacc aaatactcat
     2941 caaaatgatt gttttccata taagactctg gcactttttc aatattaacc aaaactgtat
     3001 cgatagcatc ttgtcctaaa tgacgtttca atacttcaac gtggtctgcg tctgtaaaat
     3061 gctcagtttc accacgttgt gtcataatat tgcatacata agctacctct gcccgagttt
     3121 ctaaaagagc ttgtttaatc tctggaataa ctaggttagg taaaatagag gtgaaaagtg
     3181 accctggacc caacacaatc atatcacttt ctaaaattgc atcaacaact ttgcggctgg
     3241 ccgtaggagt ctcttcatta taagtattag tgacatacac atggtcaatc atgcctttat
     3301 aatcggctat ttgactctct ccaaccactt catggccatc tttaaaaaca gcatgcagag
     3361 tgagcggatg ttcactagat ggatatatct ttcctgttgt atgaaagaac tgtgttaaac
     3421 tctgcatagc gttataagtt gaaccttgca tttcagcaac accagcaata ataagatttc
     3481 ctaaaggatg acctgcaaaa tccccatcac cttctgcaaa acgatattgg aaaatttgtt
     3541 cgtaaaactt cggcatatca ctcaaggcaa ccaaaacatt tcttaaatct cctggcggtg
     3601 ttagttgcat aactgagcgc aactcacctg acgaaccacc atcatctgcc accgtgacaa
     3661 ccgctgtaat ctcaacatct tctaagcgaa gacttttcaa aataactgga attcccgttc
     3721 ctccgccaat aacggttatt ttaggttttc tcatgaacga ttcaccgtct ccttacgctt
     3781 atttttatca cgatggcttt cattcactgt ccaatctgct tttaaatctt ctgataatct
     3841 atgagcaaaa gcggtgctac gatgttgccc acctgtacat ccgattgcca cagtcaaaac
     3901 agatttccct tctttttgat agcctggtaa gattggcttt attaaggcaa gaagatggtc
     3961 ataaaaatcg tctgattcat caaaactcat gacatagtca taaacctcag tatctaaacc
     4021 tgtcttatct cttaattctg gtttatagta tggatttggt aaaaaacgca catcgaatac
     4081 taaatccgca tccaaaggta taccatattt aaacccaaaa ctcataactt caatacgaaa
     4141 agaactttga ctatcttgat ttgagaattc ttcagaaatg accttacgta gttgtcttgg
     4201 tgttaattca ctagtgtcca caacgttctg actcatactt ttaagcggtg ctaatagttc
     4261 tctctctaga gaaataccat ctaacactct gccgtcggca gcaagagggt ggctacgacg
     4321 tgtctcttta tatcgtgaca ctagctcggt gtcagtagca tctaaaaata gaatcttaaa
     4381 gttaatatta tcattaattt caaggctatc caaaatagag ttaatttctc taaagaaaag
     4441 acgactacgc atgtcaacta ccatagcaat cttgcttgta tctcctgatt gtgctgctaa
     4501 ttctaaaaac ttaggcacta gagtaggcgg catattatct attgtaaaat atcctaaatc
     4561 ttcaaatgac tgaatagcca ctgtctttcc tgccccactc atacctgtta caatcacaag
     4621 tttgatttgt tcgtctgaca ttacgattac ctctttctct tatgttttat gccattatac
     4681 cacaaatggc tagcaattag gattttactt cagaggaaaa gaaactcatt ctaaatagta
     4741 aaatcttgtc ggaatgctcc caaatttggt aaactagacc caaaggagtt tattatgctt
     4801 tatgaagtaa cctcatcaaa cactcaaggc gttgatggaa aagtttatct ctccaatgga
     4861 aaaattgtgg agaccaatca ccctttaaat catttaccag gatttaatcc tgaagaatta
     4921 attgctttag catggtcaac atgtttaaat gcaactataa aagctatttt agaacaaaaa
     4981 ggattcaaag acctaaaatc tcgtgttgat gttaattgcc agctcatgaa ggaaaagcaa
     5041 gtaggtaagg gattttattt ccaagttaac gctgttgcta gtattgaaaa actgtcatta
     5101 tctgatagta agctaatcgt taacaaagca cacagcagat gtccgatttc aaaattaatt
     5161 tcaaatgcta aaacaatcaa tctagacact gttaaatggt cagtttgaat ctttacaagt
     5221 tagtgctttc atattataat aaaacacgtg cataatcgag gatttctcgg actggttcga
     5281 aaacttccca gaataaaaaa ctaagtgact gaaaatatga ttgaatttag aaaactaaat
     5341 tcattttttc gtgtgatctt ggtaactgcc aagatctttt ttattgccat gactattgtt
     5401 aatagtttca taaaagcata gaacattcac taatattaaa agaaaggaga caaatgaaca
     5461 aagaaaaaat tattatcgat tgcgatcccg gtattgatga taccttagca ctgatgtatg
     5521 ctattcagca tcctaagtta gaagttgttg ctattactat aacagcaggc aattcgcctg
     5581 ttgagctagg actaaagaat acctttgtaa cgcttgaatt actaaatcgt cgcgacattt
     5641 cagtttatgt tggtaataac ttgcctttac aacgtgaatt tgtatctgct caagatactc
     5701 atggaatgga tggccttgga gagaataact ttacattggc gcaaccaatt atctttcaag
     5761 aagaatcagc agattgtttt ttagcaaact attttgaaca caaaaatgat acttctatta
     5821 tagctctcgg acctctgacc aatatcgctc gagccttaca aactaatccc aaattaggca
     5881 aacattgcaa gcgatttgtt agcatgggag gaaattttaa atcacatggc aattgttctc
     5941 cagttgctga atacaactat tggtatgatc cccatgctgc acaatatgta tttgaaaacc
     6001 ttgacaaaaa aatagagatg gttggactag acatcacacg tcatattgta ttaaccccca
     6061 atcatttatc ttatatggag cgtattaatc cagacgtatc aacttttatc caaaaaatca
     6121 caaagttcta cttcgatttt cactggcaat atgagcatat tattggatgt gtgataaatg
     6181 atcctttagc tattgcttac tttgttaatg aaaatatcgc tactggtttt gatagctata
     6241 ccgatgttgc ttgtcacgga atcgccatgg gacaaacaat tgtagatcag tatcattttt
     6301 ataaaaaaga tgctaacagt aagattttaa cctcagtcaa tactaattta ttttggaatg
     6361 attttatgac cgtcctatta catgcacaaa attcagtcat cattgaagac ttagaaatgt
     6421 taggacttaa caaatgacaa aatcacctgc tcgtttaatt tcatttatct caatcgctat
     6481 cgccatcaac cttattgggg ctaatttagc tctattttta cgcctaccta tctatctaga
     6541 tactataggt acattgctaa tagcagttat cctgggacca tggtacgctg ccagcactgc
     6601 ttttctcagt gcactcatca attggatgac tactgatatt ttttcacttt attattcacc
     6661 agtcgccatc gtagttgcta tcattactgg tatcttgatt aaaaggaatt gtaaaccctc
     6721 tagcctattg tggaaatcac tgattatttc tttaccagga actattattg cttcagttat
     6781 aacagttatc ctttttaaag gaattacctc tagtggctca agtattatag cacaattctt
     6841 acatggaatt ggattagaca tgacaagtag tttgatactt gttcaagttg gaacagatta
     6901 tattgatcga ctaatcagtc ttatccttgt cttttcgaca atcacattgt taaaaaaaca
     6961 tagtcccaat ttaattaacc aacgattcta aagaccataa taaaaaagcg cactagctag
     7021 cgctttttta ttatggtttg atacgatgta acatacgtgg gaatggaata gcttcgcgaa
     7081 tatgtttggt accagcaaca aaagttacca tacgttcaat acctatacca aaaccgccat
     7141 gaggaacaga gccgtattta cgaaggtcaa ggtaaaagtc gtattctgat ttatccatac
     7201 ctagttcatc cattttagcc acaagagcat cgtagttgtc ttcacgcatt gagccgccaa
     7261 tgatctctcc ataaccttct ggagctaaaa gatcagcaca aagaacgcgt tctggatttc
     7321 caggaactgg tttcatataa aatgctttaa agcttgctgg gtaattcact acaaatgtcg
     7381 gcactccaaa gtaattagaa atccaagttt catgtggtga accaaagtca tcaccatgct
     7441 ctaaatgttc ataatctgta tcttcatctg cttcatgctc ttgaagaagt gtaatagcat
     7501 catcataaga aacacgcttg aatggctctg cgatataacg tttgagagcc tcaacatcac
     7561 gttctaaaat atcaagtgct tgaggcgcac gatctagaac gccttggatc aatgctttaa
     7621 cataagcttc ttgaaggtca agtgattcct catgtgacaa gaaagaatac tcggcatcca
     7681 tcatccaaaa ttctgttaaa tggcgacgtg ttttagattt ttcagcacga aagactggac
     7741 caaagtcgaa aactcggcct agagccattg ctccagcttc aaggtataat tgaccagatt
     7801 ggctaagaaa tgctggtttc ccaaaatagt ctgtttcgaa taactctgtt gaatcttctg
     7861 ctgcattttc tgataaaatt gggctatcaa atttgataaa accattttgg tcaaagaatt
     7921 cataagttga ataaataata gcgttacgaa tttgcataac agccatttgt ttacgagaac
     7981 gcaaccaaag atgacggttg tccatcaaga agtctgtccc atgctcttta ggagttatag
     8041 gatattcatg tgactctcca atgacttcaa ggtcagtaat atcaagctca tagccaaatt
     8101 ttgaacgttc atcctcttta acaatccctg ttacataaac agatgtttct tggttgagac
     8161 gtttaataac gtcaaatttt tcaagacctg attcttcacc atatttttca atgaagtttg
     8221 gtttaaaagc gacaccttgg aaaaaggcag aaccatcgcg caactgtaca aacgcaatct
     8281 tacctttacc tgatttgtta gcaacccaag caccaatagt aacttcttga ccaacataat
     8341 ctttaacatc aacaattgaa ataagttttt ttgacataag tttctctcta tttttattta
     8401 attaatttca ttacttccca taaagacatg cagtctacga acagcttctt taagggttcc
     8461 aagatctgta gcataactca gacgaacatt ttccggcgct ccaaaaccag cacccgttac
     8521 taaagcaagt ccaacttctt caagaatggc atccgtgaat gctgtaacat ccgtatatcc
     8581 tttcatttcc attgcttttg taacatttgg gaaaagataa aaagcacctt gtggtttaac
     8641 aacctcaaac ccagggacct gacacaacaa tggataaatg atattaaggc gttcttcaaa
     8701 tgctagcctc attttttcaa atgactcttg agaaccattt aaagcctcaa tagtagcata
     8761 ctgtgagaca gccgtaaggt tagaagttgt ttgactgact actttagaca ttgccgctat
     8821 aatatcatga ttacccactg caaaaccaac acgccaacct gtcatcgcat aagtcttaga
     8881 aactccattt atgactatcg tttggtttcg aatggactct gaaaggcttg aaataggggt
     8941 aaaaatattg ccattgtaaa ccaatcgacc gtaaatatca tctgctaaaa ttaggatatc
     9001 atgttcgact gcccaatttc caatagcctc caactcctca gctttataaa tcatacctgt
     9061 aggatttgat ggactattaa gaagtatgac ttttgtctta tcagtcctaa cagcttctaa
     9121 ctgttccaca gttactttga agtgattaac ttcttttgct gtgacaaaaa ctggcttacc
     9181 ttcagccatc ttaatctgat cagcataaga tacccaataa ggagtcggaa taattacttc
     9241 atcaccggga ttcagtactg tcataaagaa agtataaagg ataaacttag caccagttcc
     9301 cacaaccacc tgattaggtt taagggagta gccataaaaa cgttcaaaat acgcattaat
     9361 tgctgacttc aactccggca aaccacttga tggtgtgtaa aaacttgcac gtccatctgt
     9421 tatagcctca atagccgctt gctgaatgtt tttaggagta gcgaaatcag gctcccctaa
     9481 agttaaacta agaatatctc ttccttgtac tctgagttct cttgctttag ctcccgcagc
     9541 taaagtaaca ctctcttcca tattgagtac acgttctgac aaataggtca tcactcctcc
     9601 ttttatcaac gtactactat tatttaatct tgagtcgcac tgtcaacatc ttatttttag
     9661 acaatgatct ttaccattat atcaaaaatt tagccatttc agtcaagacc ttgttaattt
     9721 tttccttaga aatttcaaaa tctctaccaa gagaatgcat gatggtttgt ccataagatt
     9781 taataaccaa tctgctatcc aaaataatta ccgctgactt ttgttcttct ctccttgtag
     9841 tacgtcccaa ggcctgtttt aattttaaga cagtcattgg taaagaatag tcataaaaga
     9901 agtttttccc ctcttttgtt aaactctgcg atagtttctg gataaaatag tctttgggat
     9961 tatcaaaagg caatcgcgtg ataacttcaa taagacgatc acgatgtaca aagtcaacac
    10021 cttcccaaaa agatcctgta cctaaaagaa gattgctttc tccacgatca aaacgttttt
    10081 taacgttata agctaagcca tttttatctt gagtaagatg ttttatctcc cacttatcca
    10141 aataatcaga taccataaac attgtctgtt tagaagttaa taagactaag gtaggctgct
    10201 ttaacgtcat tatatcttgt aaacgcttgg ctatttcgta agcatactgt tccggtgata
    10261 aatcaagtat attaggcata gaggtatcaa tccatacttt ctgattggca tttttctcat
    10321 gcgctatctt ctctgtgctt atacttgaaa atcctcctaa aagatctgac aagtagactt
    10381 tgggactgat ttgtaaagta gctgatatca tataggtctt ttttgtggga gggaggaact
    10441 ctgaaaattt aagaaaatct tctctcgccc cacgtaaaat ggttaaacgc ttctcattga
    10501 ctttatcagt ttcaaaccaa tagttagtat aagtagctgt gaaaaaagtt tgtaaatcat
    10561 ctaatcctac tacttctaaa gcgtttatac tattttttag acgtttcaac caggtctctg
    10621 aaaattcaaa agaattatga cgataaaaaa gttctgctgc atgacttaac tcataagaaa
    10681 tactttccag aaggcgcttt tgtaataacg gaataggact atcaattatt ttttgaataa
    10741 cctgcaattg gtgactaata tcaagttgcc cacgagaaaa attttccagt cccaaaacaa
    10801 gtttctgagc ttcatcaaaa actaaaacct tcccctttgc aaaatctttg tcatcctgaa
    10861 cacgctcaag aaaataagca tgattaataa taacaagttg actttgtgca actttatcat
    10921 aactgcgttt ccaaaaatcc agatcataaa aaagtgatga ttgagtcact tctccatcgt
    10981 gttttagctg gtcaaagtaa gattctaatc gttgtttttg tttaatttca tccaaatcac
    11041 ctgtagtcgt ttccgtcaac cacactagca gttgcatttt aaaacgattt atcaatcgat
    11101 ttcgatcctg cacttggagg ctcttataaa acgcatctaa cttcaagtaa tttctcggtc
    11161 ctttgatact gtgacaggga atatgaaaaa gttcttgaat gtgctttatt tctttagcca
    11221 ttatttgatc ttgcaaaatc tttgttggaa cagatactat tatttgttgt ttttgagact
    11281 gatccaataa aggcaagaga tatccatatg ttttaccaag tcctgattga gcttctaaaa
    11341 acgttgttgt ttgatcatta aaatgcgctt taaccaagtc agcaaaaaca atttgcttag
    11401 ggcgagcatc catacctaac aaagccatgt taacttgaaa atcactagat aatttcttag
    11461 gctttccaac agctttttct ttttttaaga ccaatccttg ccagaaataa tattctttag
    11521 gattaacgaa atcagcttct tgataagctt cttcaatcaa taaataggat tcgaacaaaa
    11581 gattatcagc aaatgttaaa atggtctcca acacttcttt aggtaaacta ctaattttag
    11641 cttttaactt aatgaataac tgtgctgtag ctcttgcatc tgcaatagca gtgtgcgcat
    11701 ctgttaattc aatatttaag ctttctgcta atgcccctaa agaatatttt tctaaacagg
    11761 gataaaacac ttgtgataac tctactgtat ctatccttgg tgtacgtaac tcgaagcctt
    11821 ctaaaaatag ttgctcagcc aataaatttg catcgaactt tacattgtgg gcaacaaaaa
    11881 tacaatcttc aatcaattga taaatgtgat gcgccacttg accaaaatcc ggagctttaa
    11941 ccaactgctt atccgtaata cctgtcaaat gaacaatatg ctcatccaaa gattcatgag
    12001 gattaacatc tgtttcataa ctatcaataa ttttatttcc ttggataata acaatcccaa
    12061 cctgaataat agaagcattg ggacctgctc cagtagcctc caagtcaact actgcatatt
    12121 ttctaagttt tttctgggtc atagtaagtc tattatagca cgctatatct ataaaacaaa
    12181 acatgactgt acctcaaaga tgccatcact ccaataacca agataagata attttttaag
    12241 aaatataaaa aacactgtga gtttcactca cagtgttagc attaatcatt tttaatggaa
    12301 taccatacca ccatcgacaa ctattgtttg tccagtgata taatttgaat catctcccgc
    12361 taagaagcct actgcattag caacatcttc tggttcagac aagcgtttca aagtgatatc
    12421 ttttgcaaac tgctccatac cccattcatc atctttgcct gcattcttac caacttcatg
    12481 ggcaatatca aacatcatag gtgttttaac aatacctggt gcgtaggcat tgactgtgat
    12541 tccttctgat gccaaatcct ttgccaaagt ctgtgtaata ccacgaactg caaattttgt
    12601 accaccataa acagtcaaat taggattccc ttcacatcct gcttgtgaag ttgcatttat
    12661 aatttttccg ccatgaccta attctctaaa atgtttttga gctgcttgac ttccccaaat
    12721 agttcctccg acattgattg caaaagcctt ttcaaactgc tcttcagtaa tagtgtcaag
    12781 aggggttgtt ggggcaatac ctgcattgtt aacaacaaca ttcaaatcac caaaagtatc
    12841 tacaactttt tggaaagcat caaagacttg atcacgtttt gaaacatctg ctacaactgc
    12901 aacagcatcc tcaagtgata gtttatcaac cgcagcctga gcagtctctt cattatagtc
    12961 taaaacgcct attttaaagc cgtctgcatg aagacgtttc gcaatagcaa atccaattcc
    13021 ttgaccagca ccagtaacaa tagcaacttt tgacatatct tccacctcat attctttagt
    13081 caaccaatta ggttgctctc aaaaagtcag tgttcttaaa ctaagttcag ctttaggctt
    13141 ggcttattga ttacttttat gatacacctt tatgaaaacg gtgtcaagaa attgctgtga
    13201 gatcccatat tttctgaaac tagttgccat ttattttccg ccactctttt tttaacaaag
    13261 tcttgatttt ttggtacact atcattatga aaatctataa aacaataaac cacattgctg
    13321 gtgaaaatac ttattacctc gttaatgatc aagctgttat cctcattgat ccagggagca
    13381 atggccaaga aatcattgct aaaattaaat cctttgaaaa acctttagtg gctatcctct
    13441 taactcatac tcactatgat catattttta gtttagattt ggtcagagat acttttgata
    13501 atccacctgt ttatgtttca gaaaaagaag ccgcttggct atcttcccca gatgataatt
    13561 tatctgggtt aggccgtcat gatgatatca ttaatgttat agcaagaccg gcagagaact
    13621 tttttaaatt aaagcaaccc tatcaactaa atggttttga attcacagtt ttaccaacac
    13681 ctggtcattc ttggggagga gtctcatttg ttttccactc agatgaatta gtcgttactg
    13741 gcgatgctct atttcgtgaa actatcggtc gtactgactt acctaccagt aactttgagg
    13801 acctcattac tggtattcgc caagaactat tcaccttacc tagtcattat agtgttcacc
    13861 caggccatgg catgaataca actattggcc acgaaaaaaa ctttaatcct ttcttccaat
    13921 gaacataaaa cgtcactcta tttttctttg gttaacaaca agtatccttg ctattttaat
    13981 ggccgtcggg atgaccattt acttcaatac ttaccaagcc tccccattag ctaggagaat
    14041 tgctaaaacc gctacaaacc ataagaacta ccttcttttt agaagcaagg gtaaggtaaa
    14101 agcaaatatt attttctatc aaggggctct tgtggaagaa gaagcttatt ctcaacttgc
    14161 tagagattta gctgataaag ggtataatac ctatattttg aagacacctt taaatttgcc
    14221 agttttatca ccttataaag ctaaaacaat tattaatcag aaccacttaa ctaatgttta
    14281 ccttgctgga cattctttag gaggagttgt tgctagccaa aatgccaaag tagcccctgt
    14341 tagagggctc atattattag cttcctaccc ttcaagtaaa tccgacctat ctcataaaaa
    14401 gttaagagta ctctccatta cagcaagtaa cgatcatatt ttaaactggg aaaaatatga
    14461 ggaagctaaa aaacgcctcc caaattcaag tacttttcga actattgtcg ggggaaacca
    14521 tagccgattt ggtaactacg gacatcaaaa aggggatggt aaagctaccc tttctcacaa
    14581 atcatctgaa aaacaattgg ctacgtttat ttcaaatttt atccactagc atgatatcta
    14641 caattaataa aaagagatga aa
//
LOCUS       DATROY010000040        14483 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.040, whole genome shotgun sequence.
ACCESSION   DATROY010000040 DATROY010000000
VERSION     DATROY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14483
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1173
                     /locus_tag="VBD11_000978"
     CDS             <1..1173
                     /locus_tag="VBD11_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6953940.1"
                     /translation="HYLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQ
                     LNNSFTFTNRDFISSSLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIP
                     VTLVVLFSLVSSVIIFVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIEL
                     DSQDEFQYLSVQINQMVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETI
                     LITSHYDSQLTERIVIQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTY
                     TISVSPELEHMRVPKLFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGS
                     GISLARQKAIRTMLRSNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            1177..1950
                     /locus_tag="VBD11_000979"
     CDS             1177..1950
                     /locus_tag="VBD11_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6953941.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            1962..2999
                     /locus_tag="VBD11_000980"
     CDS             1962..2999
                     /locus_tag="VBD11_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6953942.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(3221..3718)
                     /locus_tag="VBD11_000981"
     CDS             complement(3221..3718)
                     /locus_tag="VBD11_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6953943.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(3718..4755)
                     /locus_tag="VBD11_000982"
     CDS             complement(3718..4755)
                     /locus_tag="VBD11_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6953944.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            complement(4766..5221)
                     /gene="nrdI"
                     /locus_tag="VBD11_000983"
     CDS             complement(4766..5221)
                     /gene="nrdI"
                     /locus_tag="VBD11_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6953945.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(5378..7780)
                     /locus_tag="VBD11_000984"
     CDS             complement(5378..7780)
                     /locus_tag="VBD11_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6953946.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            8040..10256
                     /locus_tag="VBD11_000985"
     CDS             8040..10256
                     /locus_tag="VBD11_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6953947.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            10266..10709
                     /locus_tag="VBD11_000986"
     CDS             10266..10709
                     /locus_tag="VBD11_000986"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6953948.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            10964..11887
                     /locus_tag="VBD11_000987"
     CDS             10964..11887
                     /locus_tag="VBD11_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6953949.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            11914..>14483
                     /locus_tag="VBD11_000988"
     CDS             11914..>14483
                     /locus_tag="VBD11_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6953950.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPINKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     A"
BASE COUNT         4738 a         2404 c         2809 g         4532 t
ORIGIN      
        1 cattaccttg cactcattaa gccaattata gttaataaaa aagtttcagg ttatgccttt
       61 ttattgatga atgggaaaga ctttttactt ccaacgaagg ctattaactc agacttgatt
      121 attgccgatc agctaaataa tagctttact tttactaatc gtgattttat ctcctctagt
      181 ttagataagg tagatagtca attcttaact agatatttta gttttcacga tcatcgagct
      241 tttgttgtta ggaaagtagc tctgcaagat aatatcctac tttatatgta tcgtcccctg
      301 ataccagtaa ctctggtcgt ccttttttct ctagtatcat cagttattat ttttgtgatt
      361 ttacgacgaa aatctagagt tctagccgat cgcatcgcag taaaaaattc tggcgccata
      421 aatcaaatgg tgctagatat ggatgccatt tcccgccaag aaaaatctag tattgagctt
      481 gatagtcaag atgaatttca atatctatct gttcaaatca atcagatggt ttcacggtta
      541 aaagatttac atgagaaaac tctcgatttg gaaacacaaa agttgctgtt tgaaaagagg
      601 atgttagaag ctcagtttaa tcctcacttc ctttataata cccttgagac aattttaata
      661 accagtcact atgattcaca attaacagaa agaatcgtta tacaactaac aaaattattg
      721 cgctatagcc ttagtggtag cacagaggca gctgtgctta aggatgattt agcaatcatt
      781 gaatcctacc ttttgattaa tcaagtgaga tttgaagaat taacttacac tatttcagta
      841 tctcctgaac ttgaacacat gcgtgtacca aaacttttct tgttaccgct aattgagaat
      901 gctattaaat atggcttaaa agaacgtcat gatgtagcaa taaacataga tatttggcaa
      961 gatagtgatg gaatttggtt tactgtttca aataatggat cgggtattag tttggctagg
     1021 caaaaagcta tacgtacaat gttaagatca aatcactcgc atcatgggct tattaattct
     1081 tatagacgat tacaatatca attttcaaca gtattgttag agtttacgaa gacagatgat
     1141 gcttttcgag ttagctatat agtaaaggag tgagttatgt atagattact tatcgttgag
     1201 gatgagcacc ttattcgtaa gtggttgcgc tatgctattg attatcaatc attgaatatt
     1261 ttagtggttg gtgaagcaaa agatggtaaa gaaggagctc aattaattca ggaagagcag
     1321 ccagacattg ttttaagtga tattaatatg ccaattatga cagcattcga tatgtttgag
     1381 gcaaccaaag gacaatctta tgccaaaatt attttatctg gctatgctga ttttccaaat
     1441 gcgcaatcag ctattcacta tggcgttctt gaatttttaa caaaaccact agaaaagcaa
     1501 gctttgattg attgtttaaa aacaattatg gctagaattg aggaacataa ggagaagcac
     1561 ctacaagagc atacagaatt gtatttacct ctaccacaag ctaatgacca agttcctgaa
     1621 gttattaaag atatgctagc atggattcat agccattttc atgggaaaat tgtgattagc
     1681 cagttagctc acgatttagg ttacagtgaa agttatcttt acacagtcac taaaaagcac
     1741 cttcatataa ctttgagtga ttatataaat caatatcgta ttaatcaagc cattcagttg
     1801 atgtttagag aaccagacct aatggtttac caaattgcag aagctgttgg tatttatgat
     1861 tatcgttatt ttgatagggt ttttaaaaag tacttgggtc agacagtaaa agcgtttaag
     1921 gaagaacata tttttaaaca gatggattag gtaggtgaaa gatgaaagaa aaacagtcga
     1981 aaaggcttat ttatatacta ctgattgttc ccattatctt tataagtgtt tttacataca
     2041 gtattagcca gccttctaaa ctacttccac caaaagaatt agttattcta agtccaaata
     2101 gtcaagccat tttaacagga acgattccag cttttgagga aaaatacggt ataaaagtta
     2161 agcttattca aggtgggaca gggcaactaa tagatagatt aagtaaggag ggtaagcagt
     2221 tgaaggcgga tattttcttt ggaggaaatt atacgcaatt tgaaagtcat aaggcattgt
     2281 ttgagtctta cgtatcaaag aatgttcata ctgttattcc agactatatc catccgagtg
     2341 atacggcgac accttatact ataaatggga gtgtcttgat tgtaaataac gaattagcta
     2401 agggacttac catcaagagt tatgaagatt tattacagcc ttccttaaaa ggtaaaattg
     2461 cctttgcaga tccgaatact tcctctagtg ctttctcaca actcactaat atactcttgg
     2521 ccaagggtgg ttacaccaat ccaaaagcgt ggaactatgt taaaaagcta caacataata
     2581 ttaatgctat caaatcttct agctcttcag aagtttatca atcagttgca gaaggaaaaa
     2641 tgattgtggg gttgacttac gaagacccta gtgtcaattt gcaaaaaagt ggtgccaatg
     2701 tttctattgt atacccgaca gaagggacag tttttgtccc atcttcggtt gcaattataa
     2761 agaatgctcc ttctatgaaa gaagcaaagt tatttattaa ttttatgctt tctttagatg
     2821 ttcaaaatgc ctttgggcag tcaacgagta accgacctat tcgtaaagat gcccaaacaa
     2881 gtaatggcat gaaagcttta aaggatatcg ctactcttaa agaagattat cgctatgtca
     2941 ctaagcataa gagccaaatc cttaaaacct ataatcgcat tcgtagaaat gctgattaaa
     3001 tgaaaatata gaaaaactcc tcggaatcat tgattccgag gagtttaatc atcgtcctcg
     3061 tcgctttcga aatctttttt ccaataacga taaagactaa tgcatgccag aaatccggct
     3121 ataataggaa cgataataaa taagaggtca atcactgtat ccatgggcaa tatactccaa
     3181 tgataagaag tcattattgt ttatagtgcg tacaataatg ttaaggtaaa acgtcggtca
     3241 ttataagttt tttgacatcc tctaaactca tagattttgt taatattttt tcaggtatct
     3301 cttttaagaa gtctttctca agccaccacc ttgcaagaga atcttctcca aattctaaag
     3361 atttaagtcg agttgaatgt cttttaacag tttcttgaaa gcttaaatca tagtaataag
     3421 cataagtatt gttggggtta tgttttaaaa tatgtttcca aacaggcgta taccaatcag
     3481 aacgaagtat gccttcaagg atgatgtagg aacagttatg gtaaccataa tttagtaaat
     3541 tgatcaaaag gggaatggta gtagtatttt caccatcctt agttcctaac atctctctac
     3601 gaaggtaatc ctgagataag agaagggtat cttcccctaa ttcggcttga agttgtttag
     3661 ctattgtaga ctttccgcta gctgaatttc ccctgataat aatgagcgtg gctaccatta
     3721 gttcaccata ttgcttttga gataggcatc cactaagagt tcagttgctt gaatagaatc
     3781 gatatgggta cgttcataag aatgactaga ttcaatacct gcaccaagta gcgcgtgttt
     3841 aacttccgca ccagcacgca tggcagcgga ggcgtcacta ccataataag gataaatatc
     3901 aagtttataa ggaatattat tgttttcagc tagagaaaca agatgttgac gtaattcata
     3961 atgataagga ccagaagcat ctttaacaca gatagagaca gtatattcgt cagtttcttg
     4021 atcgtctccc atagctccca tatcaactgc tagatattct acagtttcat ttgggatgct
     4081 tgaatttgct ccatgtccta gctcttcaaa ggcactaaag tagaaatgag tagtataagg
     4141 aagttgaatg tcttctttct tgtaaacaga aagaagttcc attaggatac cagcggatac
     4201 cttgtcatct aggtaacgtg atttaataaa gccgctgcct gttatgattg tacgcggatc
     4261 aaatgaaatg aaatcaccga cctggatgcc taaagcacgt gtttcgtcag cagttgttac
     4321 tttctcatct aaacgaattt ccatatttgt ttgattacgt tcagcagttc cagcatcttt
     4381 gtaaacatgg acactagttt gatgaataag agcagttcca gaaatttctt taccattttt
     4441 tgagaggtgt atagtacagt tttctccttc aatggcattg tatgtgtatc cgccaataag
     4501 gtcaattttt aaccgaccat ctggtttgat ggccctaacc atagcaccaa gtgtatcaag
     4561 gtgagcagtt atcatgcgat gtttagtatt attttttcct tttagagaaa ccatgacgcc
     4621 tcccttgttt gtgcgaattg gtgagtagcc aaacgcttcc aattctttga tgatataggt
     4681 catgattgtt tgggtaaacc ctgttggtga agggattgat gtcagggttt taatgtagtt
     4741 taatgttgtt tccatactat ttcctttagg ctttaaagtt cgataatgtt tcaacgataa
     4801 tatttgcgat acgttcaacg tcgctagaag ttcctcgcaa ttcaaagtct cctaacatag
     4861 gaaatccaaa ccgttcagag tattgttttg cagttaaaca gtactgattg ttaaaatttc
     4921 tatttccaga gccaacaatt ccgatacaat ttttgacatt attgtgtgca gctataaaat
     4981 caccaagtgg aatggtaaga atttcttgat cgccattgtc aatgccattt cctccctcga
     5041 ggtaagtggg taatagtgct acgaaaggtt ggtcaacagg aaaggtttga tgattttgtt
     5101 ccttgatatt aatagtcgaa acctgacagt tatgccaagt tgacagttgt tcactaagac
     5161 gtttgacaaa gctttgagta ttgccgctaa gactgatata gactagggtt aatttaggca
     5221 tttagtactc cttaaatcta aatttcttgt tatcagttta gcaaaatcat gtcagataaa
     5281 caagctagag tggtatctca atatctgact gtttagagag aaaatctatt gttgagacta
     5341 aaaactgagg ttgattggcc tcagttttta gtgatgacta tatttgtttc tttttaagta
     5401 aatttcccac gagtccaagt attgcgacgc caaagaaagc cagataagat tgtttttctt
     5461 gtccagtttt aggtaatgtt ttttctttct gttgtgtata gatagtgcct gcgatatttg
     5521 gagaagcttt tgcttttgta taagcagttg caaccatgtg gtgaattttt tgtttctcta
     5581 tttggcttaa cttttcttga tgtgatggct cagtaaggtc tactttttct tgttcagtat
     5641 agacaaatcg ataatcacca aatccttcat ctttggtaga actagcgata tactgaatat
     5701 ccttgctatt agtaacaaga tttttagctt tatcggctgt ttggaatcgt aaatcaaggc
     5761 ctttaataga ctctgtgaag ccccagttat tatcagctgt aggattgatg gttttctctg
     5821 aaataatata gtttataata gtttggcgat tttctaagtt cagcaaacgg ttcattgtgg
     5881 catttttagc cccagggaag gtaccactag cacggtagtt attggtcaca atcataaact
     5941 cttgtttgtc tgcaacaggc ttaccattgt acattaaatc gcgtactcga ctagcttgac
     6001 tattaacaac ttttccatct ttgttgtatt tattaggttg tgtaatatca aatttataag
     6061 tcaagccatc aataacgtca aaattatagg tacgatattc ggtattgata atattttgcg
     6121 gtgctttatt gttagggtca atttggttaa attgaccagc ggacatttct aaccattcac
     6181 gtaagtctgc tccggtcact tttaaaagtg cagtaacatt atcataaagg taaagatcag
     6241 ctacgttttt aattgcaaca ggtccagctg gaatatcagt atatgcggta gcatcgccac
     6301 gtgttccggc tttaaatggt gctgcagcag atagaagtgg taaatttgcc tcaggtgttc
     6361 cttttaattc atttgcaacg taccaacgtt gagcgttgtt aacaatttga actgaagggt
     6421 catctttaac tagtgcaaag taacttgtaa ttggcgcagt tgttgtacct acttgttggc
     6481 gaacatagtt gatagtgcca tcgtgtgctt ccttagccaa tgcaatgata cgctcgtcag
     6541 cagttgttga gttcatatca attttacgga ttttagcact gctttcggag acttgccatt
     6601 ttccattagt ataactaagt cctaaatcaa taataccaag gtgatccccg tacttgcctg
     6661 ccattgtaac aggtgttcca tttatttttc cattgatacc atcaactcca gtgtattttt
     6721 catagaagcc agtaccgtta cctgatggaa attcagcgtg tgagtgtccc gtaacaacgg
     6781 catccactcc cttgatgctg gcaatttgat agccaacgtt ttcttcacct tcttcatatc
     6841 gatcatctcc aatgccagag tgtgataaaa cgagaatgac atctgctcct ttagcacgca
     6901 tagtaggaac gatttcttcg attgctttaa ctgaatcttt gacaataacc tttccctcta
     6961 agttagcctt gtcccagttt aagatttgag gaggtacaat tcctgtaatt ccaatattta
     7021 gtgcaacagc tcttcctgtt gaatctttga aggttttagt aatgatgtca taagttttaa
     7081 aaactggttg atgtgttttg aaatccaaaa catttgcgtt gaggattgga aggttggctg
     7141 tagcaatgat ttttttgagg taatctaaac cgtaattaaa ttcatgatta cctagtgttg
     7201 atgcatcata accaagagca ttcatggctt ggtacatagg atgttcttca ttttctgcga
     7261 caggtttaac gattgcttta taagttccta aaggtgtccc ttgaatgaca tctccgttat
     7321 caactaagac ggtattagga ttttcttttt ttgcttcttc gataaggaca gcagtttttg
     7381 ctaaccctat tttttgagat tctttatctt gataataatc atagttaact aaattggtgt
     7441 gtaggtctgt ggttgacatg atgcgaaggt caactttttc accttcaata ggtttgagat
     7501 caccagttgt ggtttctgtg gcaacaggct gtggattttg attattggca gtggtatttg
     7561 tgcttgtggc tgttgaagca tcagttattg cagttggagt agaagtaacg tttgactttt
     7621 cctctgcaat tgtgctagtg ggggtagttg attcaacctt tgtagcggtt gaacttggtg
     7681 ttgtaacaat gtcttctgct tgaacagcta aaccacttgt tgttgtagct gttaagacag
     7741 ttaaagcgaa tatgcttttt gaaaaataat gttttgacat aaatctcctc cattaaaaca
     7801 ttacatgtat attatcctaa tctaaaaact attatagctt attgaaaacg tttaatcaat
     7861 ctcatttacg aattattttt agtaattaca ctaaaaacaa tcgtttattt ataaaggata
     7921 acttggttag acacatttac ttggtattta attctttttg agcctctaac ttcttttatt
     7981 tttaggttaa aaagtgataa aataggtaca atctatcact atgattaaag aggataacta
     8041 tggtcaaaga aattaattta acaggagaag aggttgttgc aatcacatcc cagtacatga
     8101 gtgagacgga tgttgctttt gtaaaatttg ccttaaatta cgcaaccgca gctcattatt
     8161 atcaagcaag aaagtcgggc gagccctaca ttattcatcc aattcaagta gcaggtattc
     8221 ttgcagattt acaccttgat gccgttacgg tagcttgtgg ttttttacat gatgttgtag
     8281 aagatacgga aattaccctt gatgagattg aaactgattt tggcaaggat gttcgcgata
     8341 ttattgacgg tgtaacaaaa ttgggtaaag tggagtacaa atctcatgaa gaacagttag
     8401 cagaaaatca ccgtaaaatg ttaatggcta tgtctaagga tatccgtgtt attttggtta
     8461 agctcgctga tcgcttacat aatatgagaa cccttaaaca cctcagaaaa gataaacaag
     8521 agcgtatttc acgtgagact atggaaatat acgctccttt agcgcatcgt ttggggatta
     8581 gtcgtatcaa gtgggaatta gaagatttat cttttcgtta cttgaatgag acggaatttt
     8641 ataagatttc tcatatgatg agtgaaaaac gtcgtgaacg cgaagagttg gttgatataa
     8701 ttgtcgataa aatcagatcc tatactgagg aacagggttt atatggtgat atttatggta
     8761 gaccaaaaca catttattct atttacagaa aaatgcgtga taaaaagaaa cgctttgatc
     8821 agatttatga tttaattgcg atacgctgta ttatggaaac tgctagtgat gtttatgcca
     8881 tggtggggta tatccatgag ttatggcgtc cgatgccagg aaggtttaaa gattatattg
     8941 cggcaccaaa ggccaatggt tatcaatcta ttcacacgac ggtttatgga cctaaagggc
     9001 caattgaaat tcagattcga actaaagaaa tgcatcaagt tgccgagttt ggtgttgcag
     9061 cccattgggc ctataaaaaa ggaatcacca gtaaggttaa tcaagcagag caatcggttg
     9121 gtatgggatg gattcaagaa ttagttgagc ttcaagatga atcaaaagac gccaaggatt
     9181 ttgttgactc tgttaaggaa gatatcttta cagaacgcat ctatgttttt accccaaatg
     9241 gtgctgttca ggaattgcca agggaatctg gaccaattga ttttgcttat gctattcaca
     9301 cgcaagttgg agaaaaagct actggcgcta aagtaaatgg acgtatggtt ccattgactg
     9361 ctaagttaaa aacaggagat gttgttgaga ttatcacaaa ccctaattca tttggaccaa
     9421 gtcgtgactg gattaagatt gttaagacca ataaagctcg caataaaatt cgtcagttct
     9481 tcaaaaacca agataaagaa acttctatta ataaaggtag agaattactg gttgattatt
     9541 tccaagaaca aggttatgtg cctaacaaat atttagataa gaaacatatt gaagaaatcc
     9601 ttccacgtgt cagtgttaaa agtgaggaag ctttgtatgc agccgtaggt tttggtgatt
     9661 tgagtccgat tagtattttt aataaactga cggaaaaaga acgccgtgag gaggaacgtg
     9721 ctaaggctaa agcggaagca gacgaactta ttaacggtgg cgaaatcaaa accgataagc
     9781 gtgatgttct caaagttaag agtgaaaatg gtgttatcat tcaaggagct tcaggtttat
     9841 taatgcgtat tgcaaaatgt tgtaatcccg ttcctggaga tctcattgaa ggctatataa
     9901 ctaaaggtag aggcgtggct atccatcgtt cagattgtca aaatttaaag agtcaggaga
     9961 actacgaaca acgtttgatt gatgttgagt gggatgatga tggatctaaa aaagagtaca
    10021 tggctgaaat cgatatttat ggtttgaatc gtagtggcct ccttaatgat gttcttcaaa
    10081 ccttatcaaa tgcaacaaag ctagtatcga cagtaaatgc acaaccaaca aaagatatga
    10141 aatttgctaa tattcatgtt agctttggaa tttcaaattt agcacaattg acaactgtgg
    10201 ttgataaaat caaaattatc ccagatgttt attctgttaa acgtacgaat ggttaggaga
    10261 gcaaaatgaa aatcattatt caacgtgtta gtcaagcaag tgtttctatt gaagatgatg
    10321 tagtcggatc tattgagaag ggtttagtac ttctagttgg gattgctcca gaagatacaa
    10381 cagaagatat tgcttatgca gttcgtaaaa ttactagcat gcgtattttt tctgatgatg
    10441 aggggaaaat gaacctttca attcaagaca ttaaaggaag cgtcttatct atctcacaat
    10501 ttacactctt tgcagatacc aaaaaaggaa atcgtccagc ttttactggg gcagctgatc
    10561 ctgttaaagc gaatcagttt tatgatatct ttaatcaaga attagcaaat catgtttcgg
    10621 tagaaacagg tcaatttgga gctgatatgc aagtcagtct gattaatgat ggaccagtaa
    10681 ctatcgtttt agatactaaa aataaataaa aaatcaaaga gttgggcaaa atgcttggct
    10741 ctttttattg tggttctaat tatactcctt aacccattca tgtttataaa aagattcact
    10801 gtagcttgga aaggcatgaa taaaggttgt tgatatgaaa ttaagtgcac ttaacaatat
    10861 taaaaataga aaaatattag atttatggtt tttcgttgac actaaaataa gaatactcta
    10921 aaacgttaac tggttaagta ctttttattt taaaggaggt ttaatgaaaa ggatacggaa
    10981 aagccttatt tttgttctcg gaatagttac cctaatttgc ttatgtgctt gtactaaaca
    11041 gagccagcaa aaaaatggct tgtcggtcgt gactagcttt tatccagtat attccattac
    11101 aaaagcagtt tctggtgatt tgaatgatat taaaatgatt cgatcacagt caggcattca
    11161 tggttttgaa ccctcatcaa gtgatgttgc tgccatttat gatgctgacc tatttcttta
    11221 tcattcgcac acactagaag cttgggcgag acgtttggaa cctagtttgc atcactctaa
    11281 agtatctgta attgaagctt caaaaggtat gactttggat aaagttcatg gcttagaaga
    11341 tgtagaggca gaaaaaggag tagatgagtc aaccttgtat gaccctcaca cttggaatga
    11401 ccctgtaaaa gtatctgagg aagcacaact catcgctaca caactagcta aaaaggatcc
    11461 taaaaacgct aaggtttatc aaaaaaatgc tgatcaattt agtgacaagg caatggctat
    11521 tgcagagaag tataagccaa aatttaaagc tgcaaagtct aaatactttg tgacttcaca
    11581 tacagcattc tcatacttag ctaagcgata cggattgact cagttaggta ttgcaggtgt
    11641 ctcaaccgag caagaaccta gtgctaaaaa attagccgaa attcaggagt ttgtgaaaac
    11701 atataaggtt aagactattt ttgttgaaga aggagtctca cctaaattag ctcaagcagt
    11761 agcttcagct actcgagtta aaattgcaag tttaagtcct ttagaagcag ttcccaaaaa
    11821 caataaagat tacttagaaa atttggaaac taatcttaag gtacttgtca aatcgttaaa
    11881 tcaataggta gttatataaa gaaaggacgc aggatgaatc gtaaaaaaac agttattatt
    11941 agtgctttat cagtagctct atttggtact ggtgtaggtg cttaccaatt aggtagctat
    12001 aatgcccaaa aatcagacaa tagtgtttct tatgtgaaaa cagataaatc tgatagcaaa
    12061 gcacaagcaa cagctgcaaa taaaacgccc gaccaaatca gtaaagaaga aggcatatct
    12121 gcagaacaaa tcgttgttaa aattactgat gatggttatg taacttctca tggagaccat
    12181 taccattatt acaatgggaa agtgccatat gatgccatca ttagtgaaga attgattatg
    12241 aaagacccta gctatgtctt taataaagct gatgtcatta atgaagtcaa agatggttat
    12301 attatcaaag ttaatggtaa gtattacctc tatcttaaag aaggtagcaa acgaacaaat
    12361 gttcgtacaa aggaacaaat ccaaaagcaa cgcgaagaat ggtctaaggg tggttctaag
    12421 ggagaatcag gaaagcatag tagtgcaaaa actcaagctt tatcagccag cgtacgtgaa
    12481 gcgaaagcta gcggtcgcta tactacagat gatggttatg tttttagtcc aacagatgtt
    12541 attgatgaca tgggagatgc ctttttagta ccacatggcg atcatttcca ttatatcccg
    12601 aaagctgatt tatctccatc agagttatca gccgcacagg catattggaa ccgcaagact
    12661 ggccgatcag gtaatagctc aaaaccatca aattcaagtt catacattca tgcaagtgca
    12721 ccaagcggta atgtatcaac aggaagacat gctaatgccc ctatttctat tccaagagtc
    12781 actcacgcaa atcactggtc aaaaccagct ggtaatcatg ctacggcacc aaaacaccat
    12841 gcacctacaa ccaaaccaat caacaaagat tctgctttag acaaaatgtt gaaacgttta
    12901 tatgctcaac cgttatctgc tcgtcacgta gaaagtgatg gtttggttta tgacccagca
    12961 caagttaatg ctttcacagc aatcggtgtt tctattccgc acggtaatca tttccacttt
    13021 attcactata aggatatgtc tccattagag ttagaagcaa caaggatggt ggcagagcat
    13081 agaggacatc atattgatgc attagggaaa aaagattcta cagagaaacc aaagcatatt
    13141 tctcatgaac ctaataagga acctcacaca gaggaagaac accatgcagt aacaccgaaa
    13201 gaccaacgta aaggcaaacc aaatagccag attgtctaca gtgctcaaga aattgaagag
    13261 gcaaaaaaag ctggtaaata cacaacatct gatggttaca tttttgatgc taaagatatt
    13321 aaaaaagata caggtacagg ttatgtcatt ccacatatga cacatgagca ttgggtacca
    13381 aagaaagatt tatcagagtc ggaattaaaa gcagctcaag aatttctttc aggaaaatct
    13441 gaagcaaatc aagacaaacc aaaaacaggt aaaacagctc aagaaatcta tgaggcaatt
    13501 gaaccaaaag caattgttaa acctgaagat ttattatttg gaattgcaca agcgacagac
    13561 tataagaatg gtacatttgt aattcctcat aaagatcatt accattatgt ggaattaaaa
    13621 tggtttgatg aagaaaaaga tcttttagct gattcagata agacatattc tttagaagac
    13681 tatttagcta cggctaaata ttacatgatg cacccagaaa aacgtcctaa agttgaagga
    13741 tggggtaaag atgctgaaat ttataaggaa aaggactcta ataaagcaga taaaccaagt
    13801 cctgcaccaa ctgataataa atcaacatca aattctagtg acaaaaactt aagtgctgca
    13861 gaagtattca aacaagcaaa accagaaaaa attgtaccgc ttgataaaat tgctgctcac
    13921 atggcatatg cagttggatt tgaagatgat caattgattg ttcctcatca tgatcattat
    13981 cataatgttc ctatggcatg gtttgacaag ggtggtttat ggaaagcacc agaaggctat
    14041 acattacaac aactcttctc aacaattaaa tactacatgg aacatcctaa tgaattacca
    14101 aaagaaaagg gttggggaca cgacagtgat cataacaaag gctcaaataa agacaataaa
    14161 gccaaaaatt atgctccaga tgaagaacct gaagattcag ggaaagtaac tcacaactat
    14221 ggtttttatg atgttaataa aggttcagac gaagaagaac cagaaaaaca agaagatgaa
    14281 tcagagctag atgaatatga actaggaatg gcacaaaacg ctaagaaata tggtatggat
    14341 agacaatctt ttgaaaagca actcatccaa ttatcaaata aatatagtgt aagttttgaa
    14401 agctttaatt atattaatgg taatcaagtt caagttacta aaaaagatgg ctctaaagtg
    14461 cttgttgata ttaaaacact agc
//
LOCUS       DATROY010000041        14450 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.042, whole genome shotgun sequence.
ACCESSION   DATROY010000041 DATROY010000000
VERSION     DATROY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14450
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..924)
                     /locus_tag="VBD11_000989"
     CDS             complement(<1..924)
                     /locus_tag="VBD11_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEO6953951.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSKNDKILLNSYFKH"
     gene            1090..2232
                     /locus_tag="VBD11_000990"
     CDS             1090..2232
                     /locus_tag="VBD11_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6953952.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            2257..3513
                     /locus_tag="VBD11_000991"
     CDS             2257..3513
                     /locus_tag="VBD11_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6953953.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            3614..4678
                     /locus_tag="VBD11_000992"
     CDS             3614..4678
                     /locus_tag="VBD11_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6953954.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(4733..5068)
                     /locus_tag="VBD11_000993"
     CDS             complement(4733..5068)
                     /locus_tag="VBD11_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6953955.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(5257..6285)
                     /gene="queA"
                     /locus_tag="VBD11_000994"
     CDS             complement(5257..6285)
                     /gene="queA"
                     /locus_tag="VBD11_000994"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6953956.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            6438..7118
                     /locus_tag="VBD11_000995"
     CDS             6438..7118
                     /locus_tag="VBD11_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6953957.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            7269..7970
                     /locus_tag="VBD11_000996"
     CDS             7269..7970
                     /locus_tag="VBD11_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6953958.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(8042..8998)
                     /locus_tag="VBD11_000997"
     CDS             complement(8042..8998)
                     /locus_tag="VBD11_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6953959.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            9094..9813
                     /locus_tag="VBD11_000998"
     CDS             9094..9813
                     /locus_tag="VBD11_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6953960.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            9886..11037
                     /locus_tag="VBD11_000999"
     CDS             9886..11037
                     /locus_tag="VBD11_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6953961.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            11089..12036
                     /locus_tag="VBD11_001000"
     CDS             11089..12036
                     /locus_tag="VBD11_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6953962.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            12052..13857
                     /gene="pepF"
                     /locus_tag="VBD11_001001"
     CDS             12052..13857
                     /gene="pepF"
                     /locus_tag="VBD11_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6953963.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVTKGVHND"
     gene            14052..>14450
                     /locus_tag="VBD11_001002"
     CDS             14052..>14450
                     /locus_tag="VBD11_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO6953964.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGV"
BASE COUNT         4704 a         2258 c         2767 g         4721 t
ORIGIN      
        1 atgcttaaaa tatgagttaa gcaagatctt atcattttta gataagttta caagcgcttt
       61 atttagcaca tattgtttaa tattagaatc aatacttgtt aataacagtt caagatcaag
      121 gtcgtcgacg aggttgacct tttgttgacc ttttaaagct ttcagggcta agatagacgt
      181 ttcatatgat ctttttaaaa gtgataaaga tgatttttgt ccgataccaa ctttatagag
      241 acccttttcg cattcaaact ttgataggat atttggataa taatagtcaa acatctggtc
      301 agaaagtaaa agccagtatt gattgggata attaaaggta taaatagcag tgtcaagttt
      361 atcaaaaaag ttcaagagtg acatttcaga ttgacttaag agtaattttt gtttttgact
      421 attgatttca aaagtgagta gacgatagtc agttttttct gataggtggt attggtttaa
      481 aaattgattg aggtaatagt agtctaactc tctgccttca acaagatggt gtaagacaat
      541 agaagcttcg ttttttcgcc cgaaatccag ataatctaat tcatgctctt ttaagatgag
      601 tcgagtcatt ttttgagcaa gtaaagcata ttttcctact tggttggggt ttccactaat
      661 cccaatagta gctaataatt cacaattata atagaaagga atattgatcc ctgcttgtgt
      721 cccaaaataa gattcttgat ctgttacttc aatcatttgt cctgtctgtg ctacttttaa
      781 accaatttca tggaattctc caacacgttt tggattggta ctagcgaaaa tgattccttt
      841 ggtattaata aaattaatat cttgttgaca aacatctttg atagaggtaa cgatttgttg
      901 tgcgagttgt tttcttaatt tcattcagct taccttttgt tatatataac aaatatcata
      961 tcataaaaac gctaacattt gttctatagt ctatattttt tgttatttga ttttgttact
     1021 ataattgagt gatataaaat agtgaaaaaa taacaaagat tcacaaaata ttttcaagga
     1081 ggttttcaaa tgaaggtagt agtagctatt gattctttga aagggagtct ttcttcttta
     1141 gaagctggca atgctataaa ggagtcaatt aatgaagtaa tcccaggggc ggatgtggaa
     1201 gtacatccat tagcagacgg gggagaagga actgttgaag ctttgacgct aggtatgggg
     1261 gggacgatcg aaataatccc tgttaagggg cctcttggag aaaaagtaca tgcgagttat
     1321 ggtattattc cacagcgtca gttagctatt attgaaatgg ctgcagctgc tggtattacc
     1381 ctaattgcta ctgaagagag aaatcctctc cacacaacca cttatggagt tggagaaatg
     1441 ataaatgatg ctataagtaa aggctgtagg cactttatta ttggtattgg aggtagtgct
     1501 actaatgatg gtggggcagg tatgctacaa gctttgggat atgcattatt agataaagat
     1561 aatcaagaaa tcagtttggg agcacaagga ttggcagatt taaaatctat ctctactgat
     1621 aaggttattg aggaattaaa agaatgcgat tttaaaattg catgtgatgt aacaaatcct
     1681 ttatgtggtg ctcaaggttg tagttcgatt tttggaccgc aaaaaggtgc tgatgaagat
     1741 atgattacaa aaatggatac ttggttaagt aattatgcta ctttagctac ttcagttagt
     1801 gaaaaagcag atgcaactat tgaaggaaca ggtgcagcgg gtgggcttgg atttgccttt
     1861 ttagccttta caaatgccac cttagaacct ggtattgata ttattctatc agaaattaac
     1921 atagaaaaag cgatttctga agctgattta gtagttactg gtgaaggtcg tttggatggg
     1981 caaacggtga tgggaaaagc tccaattggc gtagcaaaat tagctaaaaa atatggtaag
     2041 aaagtagtgg cattctctgg atctgtcacc gaagatgcta tactttgtaa tcaacatggt
     2101 attgatgctt tttttccaat cgtacgacgc cttatcagct tagatgaggc tatgtctaaa
     2161 gaggtagctt ataaaaatat gaaagaaact gctactcaag tctttagatt aatcaatttg
     2221 tataatcatt aatattgaaa ggaattctga taaattatgg aagctacatt tacaacaaca
     2281 ggggctttaa taggattagc gcttgctatt ttactgatta tcaaaaaagt tcatcctgcc
     2341 tatagtctca ttcttggagc attagttggt ggtttgattg gtggcggtga tttagttaca
     2401 attgttaata ctatggtatt aggtgcgcaa gggatgatgt catccatatt acgcatttta
     2461 acgtcaggga ttttagcggg tgctttaatt aaaaccggat cagctgaaaa aattgcagaa
     2521 agtatcatta aaaaattagg acaacagcga gccatcacag ctttagccat cgcaacaatg
     2581 attatttgtg cagtaggtgt ctttattgat attgcagtta ttacagttgc tccgattgca
     2641 ttagcaatag gtaaaaaagc taatctttct aaatctagca tactgttagc aatgattggt
     2701 ggtggtaagg ctgggaatat tatttcaccc aatcctaata caattgctgc ttcagaagca
     2761 tttaaggttg atttaacaag cttaatggta caaaatatta ttcctgcaat tgcagcttta
     2821 gtagttacga ttatattagc aaagattgtt tccaagaaaa ataacgatat ttcttatgat
     2881 agtgaggagc aagtgggctc tgatctgcca gcattcttac cggctatttc tggtccactt
     2941 gtggttatct gcttattagc tcttcgtcct ttatttggaa tcactattga tcccttgatt
     3001 gcactgccgt tgggtggtct aattagtatt ttagcaacag gttacctaaa agaaacggta
     3061 ccgtttgttg aatacggttt atcaaaagtt gttggcgttt caattctttt gattggaaca
     3121 ggtacacttt caggtattat taaagcatct aatctacaat ttgatatgat tcatctcctt
     3181 gaatttttaa atatgccgac ctttatcctt gccccactct caggtatttt tatgggagca
     3241 gcaacagcat caacgacttc tggaacaact attgcttcac aaacgtttgc tgaaaccttg
     3301 attaaatcag gggtaccggc ggtttcgggt gcagctatga ttcacgcagg tgcgactgtt
     3361 ctggattcgc tacctcatgg atccttcttc catgcgacag gcggagctgt caatatggct
     3421 ataaaagaac gtatgaaact aattagctat gaagctttga ttggattaac tagcacaata
     3481 gttgctgtcg tttactattg tttctttggg taaaatttgg atgaatacgt gatgtttatt
     3541 atcactgttt gaatagaatt ctcattgatt ttttctaatt ttcttactat aataaattag
     3601 taggagaggt gttgtgaaac aaacaaagaa agaatttatt ttaggattaa ccattattat
     3661 tacccttact ttattaatca tgctgatttt tttagtaatg tttaataagt ccagtaactc
     3721 taagttgaag cagacaaaag atagaagaat attatctact tcaaccacaa ggacatccag
     3781 tagctcacat gatcaaaaaa ttgtagaaaa gtctcttgaa aaaaagaaaa tggtcatgga
     3841 acaagatatg gaattgatga atgcctatag gctctattat gattatgccc atttgtcctt
     3901 gaaagaggtt gttctggcct atatgtctga atatggcatt gaaaaaaata gtgttgcttt
     3961 ttcttataaa aatttaaaaa caggacaaag ggacgctatg aatgatactc aggctatgac
     4021 agcagggagc acctataagt tacctcttaa catgttggta gtagatgcag tggtcaaggg
     4081 caagttgtcc atggaagaac gctttgatat taccgagact gactatgaat atattggtga
     4141 acataataat tatgttgctg ctttctcggg ggcaatgtct attgatgata tgcaaaaata
     4201 ctctttagtt tattcagaaa atacacccgc ctatgcttta gctgagcgta ttggtggcat
     4261 ggatagtgct tattcaaagt ttggtcgtta tggccaatct aaaggagaca tcaaaaatat
     4321 acaaaaaaac ggtaataaag tgacaacaga ttactatatt caagtgctag actatctctg
     4381 gaaacatcgt aaaaagtata atagtttaat tacttattta gaagaagctt ttccaacgga
     4441 ttattatcgt gctttgatac catctgatgt tgttgttgct caaaagccag ggtatgtccg
     4501 tgaggcgttg aatgttggag caattgtcaa agaagaagtg ccttatatcg ttgctattta
     4561 tactgcaggt ttaggtggct ctactcagga agattcagaa attaatggag tgggactata
     4621 tcagctagaa caattatgtt ttgttattaa tcaatggcat cgtgttaata tgaattagaa
     4681 ataaatctat caaaataata aaaacctctt agattaacta tctaagaggt ttttataaat
     4741 gtgattttat aacttgatta agtgaagaaa gggcagaaac agttttttga taagattcag
     4801 gactaatttc tgagaaaaaa tacccgcacg attgcaccgc attgacaacg gcatcttgtg
     4861 actcaatacc tttgttactt agatgaataa tgagttgcct tttgtcatct cttggttttt
     4921 ctcgtacaat ccacccacga ttttccatac gctttaaaac tggagttagg gtattcgaat
     4981 taagagatag tgcataacta agttccttca tatgccgatg atcctcttcc caaaggacta
     5041 acaagataac gtattggaga taagtcaatt gaaattcatc caaagctttt tggtaaaatt
     5101 tattgaaaag tcgatgtgtt tcactaaccg taaaacataa tcgctcgtct aaaatacttc
     5161 gattactcat tatcatattg tagcattttg gaggcgttat agtatgtcaa tcgtgttaaa
     5221 aaacgataaa actttttcct aattgaaagc ctattcttac ttaacaaaca tagcatcacc
     5281 aaaactgaag aaacgatatc gttcttcaac tgcatggttg taagcttcta gtacaaagtc
     5341 atgtccagca aatgctgata ctaacatgac aagagtagac tttggtaagt gaaagttggt
     5401 tgaaaaagcg tctacaacct tgaactgata accaggcttg ataaaaatat ttgtccaccc
     5461 tgagtcggct tttaattcac cattgaattt agaaccaatt gtctctaatg tgcgaataga
     5521 agttgtacca acagctacaa tacgtccacc agattcttta actgcattga gagtatcagc
     5581 agcttcttta gataattggt aaaactcaga atgcatttcg tgttcatcaa gattatcaac
     5641 tgaaacagga cggaaagttc caagtccaac gtgaagcgtc aaatagacaa gcttcacacc
     5701 ttttgtttca attttttcca atagttcttt tgtaaaatga agaccggctg ttggtgcagc
     5761 agcagaacca ttttcttttg catacacagt ctgatagcgg tcacgatctt ctagtttttc
     5821 atgaatatat ggcggaagtg gcatttcgcc caaactttca aggacttcta gaaaaatacc
     5881 atcatatgag aattctacaa tacgcccacc atgttctaat tcctttgtga ctgttgctat
     5941 taaccgacca tcaccaaatg aaactctgtt ccctacgcgt aagcgtttag cgggtttagc
     6001 aagcacttcc cattgatcgc cctctgtatt tttgagaagt aataactcta catggccgtg
     6061 tgtatctggc ttttcaccat aaaggcgagc gggcagaaca cgtgtgttat tcattactaa
     6121 agcgtcacca ggcttgagtt catctaagat atgatcgaaa tggctatctg tcatcgtctt
     6181 attcttatga tcaatgacta atagtttaga agcgtctctt ttttcaagtg gtgtttgggc
     6241 aataagttct tctggtaaat aaaaatcaaa atcgtttgta ttcatgattt ctcctatcta
     6301 taagtaagct tttctattat atcacgttcg gtaaaaatga cccaactaat cttcttattt
     6361 agaatgatta taaaaaataa ttgtaataat tctaaataag tggtataata attgtcagaa
     6421 agtgagaagt agagaagatg aaagaagcaa aaaatttttc ccaacaattg aatattttaa
     6481 gagctggtgt tcttggtgct aatgatggta tcatctctgt agctggtgtt gttattggtg
     6541 ttgcgagtgc aactcataac ctttggatta tttttttatc agcagcttcc gcgattttag
     6601 ctggtgcatt ttctatggct ggcggggagt atgttagtgt ttctactcaa aaagatactg
     6661 aacaagcagc agttgcgcgt gaagagaaac tactagaaaa taatccagaa cttgccaaaa
     6721 aatccttagt tgatatctac ttagcaaaag gagaaagtca cgaacatgct caatggctag
     6781 ttgacaaggc tttttcaaag aatgctattg agcatttagt tgaagaaaaa tatggtattg
     6841 agttcggtga atatacaagc ccctggcatg ctgctatatc tagttttata gcttttgcca
     6901 tcggatcaat ttttccaact ataactattc tgttattacc attttcggta cgaattgtag
     6961 gaacggtaat aattgttatt gtttcactat tatcaactgg ttatgtaagt gctaagttag
     7021 gccaagcccc tacagtacct gccatgcgtc gaaatgtgat gattggttgt ttgacaatgt
     7081 tggcaaccta tgtcattgga caattatttt caatataaaa tattaatcgt ggagttattg
     7141 tctaaactag aatggacagt aattcttttt agttttataa cgaaattaag aagtttttat
     7201 tgacaaaaat tggtatatac catataatat aatcataaag gtctatacca aataaaagga
     7261 gagtaaacat gagagttatt acagttaaga acgacattga ggggggaaaa attgcattta
     7321 ctcttttaga agagaaaatg aaaactggtg ctcaaacttt aggattggca acaggaagta
     7381 gcccaatcac cttttacgaa gagattgtaa aaagtaacct tgatttttca aatatggttt
     7441 ctattaacct tgatgaatat gttggaattg ccgcaagtaa tgatcaaagt tactcttatt
     7501 ttatgcataa acatcttttt gatgctaaac cttttaagga aaataacctt ccaaacggct
     7561 tggctaaaga tttgaaaaaa gaaataaaac gatacgatgc tgtcataaat gctaatccaa
     7621 ttgattttca aattcttgga attggtcgta acggtcatat tggttttaat gaaccaggta
     7681 caccttttga tattacaaca catgtggttg acttagctcc aagtacaatt gaagctaata
     7741 gtcgtttctt taatagtatt gatgatgtac caaagcaagc cttatcaatg ggtataggtt
     7801 ctattatgaa atcaaaaaca atcgtattag tggcgtatgg tattgaaaaa gcagaagcga
     7861 ttgcaagtat gattaaagga cctatcacag aagatatgcc agcaagtatc ctccaaaaac
     7921 atgatgatgt tgtgattatt gtggatgaag cagcggcatc aaagttgtaa agtaaataat
     7981 aaaaaagtaa ggatgaatct caatgagaaa aaattcattc ttactttttt attatgcttt
     8041 attaaattgt ccagtctaag ttaggtccag ctggaataat accggtaggg ttaatggttt
     8101 tatggctacc gtaatagtgt tttttgatat gatcaaaatt aattgtctca gcaattccag
     8161 gtaggttgta aatacgtttt gtataatgcc ataaatgtgg gtaatcatgt agcgctttta
     8221 aattacattt aaaatgacca taataaacgg tgtcaaagcg aactaaagtt gtgaaaaggc
     8281 gaatatcagc ttcggtaaat tgttctccaa caagataatg gttatcagat aagtgttttt
     8341 ctaactgatc taaagctgtg aataaggttt caacttcctt ttgataaacg ttctgagaag
     8401 tagcaaaacc agctttatag acaccattat ttatttttgg ataaataaaa ttattcattt
     8461 cgtcaatttg tccttgtaag gaatcgggat agtaatcgtc agtatttcca gtaatatgat
     8521 taaatgcagt atttaacatg cgcataattt cagatgattc attgttaacg attgtatggc
     8581 attttttatc ccaaagaaca gggactgtca cgcgtcctgt ataatcactt tgacttgctt
     8641 gatagatttg gtaaaggtat tggctttggt taatcatatc aggaataacg cctttatatt
     8701 cttgaaaagt ccaaccattc tccaacatta aagggttaac aatggagata gagatatgag
     8761 attctagatt tttaagcttt ctcatgatta atacacgtga tgcccaaggg caggctagtg
     8821 aaacgtagag atgatatcgt ccagattctg ctttaaaacc tgcatcccca ctaggccctg
     8881 ctgaaccatc tttagtaacc caatgacgga attgtgtaac ggttcggacg aatttaccac
     8941 cagttgatgc agtatcatac cattgatcaa cccatttgcc atctactaaa agtcccatga
     9001 taatctcctt tgtttttcta ttactaatta gtagtataaa gattttaaaa ggaattagca
     9061 aatgtttaac ttacagtatg gtaaactaag ggtatgcgtt tagataaatt attaggtcag
     9121 gcaggatttg ggtctagaaa tcaggttaag aaattaattc gttctaggca agtttctgta
     9181 gatggtcaaa ttgttacaaa ggataatgtt attgtagata gtggtctaca atcgattttt
     9241 gtaggaaaag agagagtctg cctaaaagaa agttcttatt accttcttta taagccatca
     9301 ggagttgttt cagcagttag agattcagaa cataaaacag tcatagactt aattagcgaa
     9361 aaagataagg tggagggatt gtatccgatt ggtcgtctag atcgtgatac ggaagggctt
     9421 ttaataatca cgaataatgg tccactgggt taccgaatgt tacatccaaa acaccacgtt
     9481 gcaaaaacgt attatgttga agtaaatggt ttcttggaaa gagatgctat tacctttttt
     9541 gaagaaggag ttgtatttga cgatggaaca aagtgtaaac cagctgaatt aacgattgat
     9601 acggctaata atgacaaaag taccgcaaga ataactatca cagaagggaa atttcaccaa
     9661 gtaaaaaaga tgtttcttgc atatggcgtt aaagttatat acttgaggcg tatttctttt
     9721 ggtgacttga ggcttgatat gaatttgaaa ccaggtcagt atcgtagatt gagagattca
     9781 gaggcagcta tcttaaaaag gtatttagat tagtgaaacg actcacttga ccttgcttag
     9841 tattcttcat atacttataa gtgattattt taaaggagaa aactattgaa ttcaaaactt
     9901 tcattgaaat tgactatttt atcgatttct atctttttga tgtctcactt agcaattgct
     9961 cctgcaattc ctaaattgta tgccttttac catactcaaa accctcatat tggactagct
    10021 tcagtagaga gtttagttac tattccagct atgatgatta ctatttttgt tattttgagt
    10081 aattttgtag tcacaaaact tggcaaaaaa aacacggtct tacttggttt atgtttaatc
    10141 ttaatgtctg ggtttatctc attttttaca agtaattttt cacttgcgat ggctagtcga
    10201 ctacttttgg gtattggtat tggtctctat aattcgctat caattagtat cataacggat
    10261 ttatatgagg ctgatgagag agccagtatg attggtctta gaacagcatc acttaatatc
    10321 ggtaaagcct taactacatt tattgttgga ttggttttag ctattggtgt gaattatatt
    10381 tatttggttt accttctggt tattcctgtt ttctttttct tttggaaaaa tgttccagag
    10441 gttgaaaatc aaacacatac ccttaaagct agtacaacat ttgatactaa agcggctttg
    10501 ttgatgctca tcacgttctt agtaggtatt gcttatatag gagcaactgt caaaattcca
    10561 accttactag tgacaaaata tcactacgcg acgagcttct ctagtaatat gttaactctc
    10621 cttgctttta gtggtatttt agttggatct gtatttggaa aattagtcaa agcattccaa
    10681 gagaagacgc tattaatcat gattctagct atgggaatag gtaatgtgct atttgcttta
    10741 gctaataatc agatcatttt tatcgtagct agtattttaa taggagctag ttttgttggc
    10801 accatgtctt ctgtattctt ttatatttca aaaaactatg caaaagaaca taataacttt
    10861 atcacaagcc ttgccttaac agctggtaat ataggagtta ttttgacacc gcttatttta
    10921 acgaaacttc caagccagct acatctggaa ccttttataa caccattttt aataacaagt
    10981 gggctgatgg tgattaatgt ctttgtttac ctagttttaa tgtcaaaaaa taaataaaaa
    11041 gttatccgaa aggatagctt ttttcgaata gtaaaagtag gagaaaaaat gttaattgct
    11101 aaagacaaac aaggaaattt aattaatcta ctcgagtctc atccgggaaa gggacagtat
    11161 ttttgtccaa cttgttgttc agctgtaagg ttaaaagcag gtcgaatcat gagaagacac
    11221 tttgcacata ttagtctaaa aaattgtcag tttcatcatg aaaatgagtc taatgagcac
    11281 ttgcaattaa aagcaaagtt atatatgagt ttaagtagag agaatgaaac tatgctcgag
    11341 catcatttgc cagaaattaa tcagattgca gatttatttg tgaatgagac attggcttta
    11401 gaagttcagt gtagccgact atcagagcag cgactcaggg agcgaacaaa agcatatcta
    11461 caggcagact ttcaagtgag atggttacta ggggaaaaac tctggttaaa gcatcgttta
    11521 acaaatctac ataaacaatt tttacaattt tctcaaagta tcggatttca tatttgggag
    11581 ttagatttga ggttagaagt actgagacta aaatatttga tttacgaaga tttacgcgga
    11641 catgtctatt acttaagtaa aacttgccct cttagtggag acgttctagc atttttgaag
    11701 tggccatacc aatcaaaaaa tctaaatttt tataaggtta aacaagacag aaatatcaga
    11761 gattatgtgc gtcagcagtt acgctatggc aatcaatttt ggttacgaaa acaagaaaag
    11821 gcctaccttt ctggtcagaa tctattaacc caagagctaa tgatgttttt tcctcaaata
    11881 cagccaccac gtgtagatac tgatttttgt caaataacaa attctctgac atcgttttat
    11941 cagaatttta caaattatta tcaaaaaaat aagaataact tggaccaaac actttatccc
    12001 cctgtatttt atgataaaat agtcactaag aattaaactg gaggatacaa aatgtcagac
    12061 aatcgctcac atattgaaga aaaataccaa tgggatttaa ctactgtctt tgcgactgat
    12121 gagttatggg aaactgaagt ggtagagctt actcaagcca tcgatgacgc aaagggtttt
    12181 tcaggtcatc tgttggactc aagccaatct ttattggaga ttacggaggt tgaactagag
    12241 ttatctcgcc gtttagaaaa agtttatgtc tatgcttcaa tgaaaaatga ccaagacacc
    12301 actgttgcga aatatcaaga atttcaagcg aaagcgacag cgctctatgc aaaatttagt
    12361 gaaacatttt cgttttacga gccagaacta ttgcaacttt cagagtctga ttaccagtca
    12421 ttccttttag aaatgcctga cttgcaaaaa tatgatcatt tctttgagaa aatatttgca
    12481 aataaacctc atgtcttatc tcaaaacgaa gaagaattgt tagctggtgc tagtgagatt
    12541 tttggagcag ctggtgagac atttgaaatc cttgataatg cagatatggt cttcccagtt
    12601 gtaaaaaatg caaaaggtga ggaagttgag cttacacatg gtaatttcat ttcccttatg
    12661 gaatctagcg ataggacggt tcgaaaagaa gcatatcaag ctatgtatag cacatatgaa
    12721 caattccagc atacttacgc taaaacatta caaacaaatg tgaaatctca aaattttaag
    12781 gctcgtgtgc atcattatca atcagcacgc caatcagctc tatctgccaa ttttattcca
    12841 gaagaggtct acgaaactct aattaagaca gttaatcatc atttaccgct cttacatcgg
    12901 tatatgaagt tgcgtcaaaa agttcttggg ctagatgatt tgaagatgta tgacgtatat
    12961 acaccccttt ctcaaatgga tatgagtttt acttatgatg aagctctaaa aaaatcagag
    13021 aaggttctgg ctatttttgg cgaagcatat tctgaacgtg ttcatcgtgc ttttactgaa
    13081 cgttggattg atgtccatgt taacaaagga aagaggtcag gcgcttattc aggtggatct
    13141 tatgacacta atgctttcat gcttcttaac tggcaagata ctttagacaa tctttatacg
    13201 ctggtgcatg aaactggaca tagtctacac tcgacgttta ctcgtgaaaa tcaaccttat
    13261 gtttatggtg attattctat tttcctagct gaaattgcat caacaacaaa tgaaaatata
    13321 ttaacagaaa cccttttgaa agaagttaaa gatgataaaa atcgctttgc aattctcaat
    13381 cattatttgg acggtttcaa aggaacaatt tttagacaaa cacagttcgc tgaattcgaa
    13441 catgccattc atgtagctga tcaggagggg caagtactaa ctagcgaata cttaaacaac
    13501 ctttacgctg agcttaatga aaaatattat ggtttgacta aagaagataa tcattttatt
    13561 caatatgaat gggcaagaat tccacacttt tactacaatt attatgtttt ccaatatgca
    13621 accggcttcg cagcagcaaa ttatttagca gagcgtattg taaacggtaa tccggaggat
    13681 aaagaggcct acctaaatta ccttaaagct gggaattcag attaccctct aaacgtcatt
    13741 gctaaagccg gtgttgatat gactagtgca gactatctgg atgcagcttt tagagtattt
    13801 gaagagcgtt tagtggaact ggaaaatctt gtaacaaaag gcgtccataa tgattaagtt
    13861 gaaaatatag ctattgaagt aaaaccagtc aactagactg gtttttgata gtgatcagtt
    13921 attttatagt ttgttttgag agtggttttg gtaaagttag ttagtggaaa actcaatagg
    13981 tggaaatacc tacccaagct atgattttta tgagaccaaa actgctattg tcaaaaaaga
    14041 agggaaatag gatgaaaaag cttactttta tttgggattt agatgggaca ttaatagatt
    14101 cgtatgtacc aattatggaa gctcttgaag aaacctatcg tcattttggc ttaatatttg
    14161 ataaagaatt aatccatgaa tatattttac aggaatcagt ggggcaatta ttggtaaacc
    14221 tttcagagga agagcaaata cctcatgaaa aactgaaagc atattttaca aaagaacaag
    14281 aaagtcgaga ttctaaaata catttaatgc catatgcaaa agagatttta gaatggacca
    14341 aagaacaaga tattcccaat tttatgtata cacataaagg agcaagtacg cattcagtgt
    14401 tggaaacctt gcagatctct cattattttg atgaaatttt aactggtgtt
//
LOCUS       DATROY010000042        14349 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.043, whole genome shotgun sequence.
ACCESSION   DATROY010000042 DATROY010000000
VERSION     DATROY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14349
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..213)
                     /locus_tag="VBD11_001003"
     CDS             complement(<1..213)
                     /locus_tag="VBD11_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-acting positive regulator"
                     /protein_id="HEO6953965.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKL"
     gene            complement(204..1541)
                     /locus_tag="VBD11_001004"
     CDS             complement(204..1541)
                     /locus_tag="VBD11_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6953966.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDTVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            complement(1629..2327)
                     /locus_tag="VBD11_001005"
     CDS             complement(1629..2327)
                     /locus_tag="VBD11_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6953967.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            complement(2339..4168)
                     /gene="glpO"
                     /locus_tag="VBD11_001006"
     CDS             complement(2339..4168)
                     /gene="glpO"
                     /locus_tag="VBD11_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6953968.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            complement(4181..5689)
                     /gene="glpK"
                     /locus_tag="VBD11_001007"
     CDS             complement(4181..5689)
                     /gene="glpK"
                     /locus_tag="VBD11_001007"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6953969.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            complement(5804..6067)
                     /locus_tag="VBD11_001008"
     CDS             complement(5804..6067)
                     /locus_tag="VBD11_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6953970.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            complement(6156..6413)
                     /locus_tag="VBD11_001009"
     CDS             complement(6156..6413)
                     /locus_tag="VBD11_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6953971.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(6425..8464)
                     /locus_tag="VBD11_001010"
     CDS             complement(6425..8464)
                     /locus_tag="VBD11_001010"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6953972.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            complement(8468..9109)
                     /locus_tag="VBD11_001011"
     CDS             complement(8468..9109)
                     /locus_tag="VBD11_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6953973.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            complement(9109..10023)
                     /gene="glyQ"
                     /locus_tag="VBD11_001012"
     CDS             complement(9109..10023)
                     /gene="glyQ"
                     /locus_tag="VBD11_001012"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6953974.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            complement(10336..10878)
                     /locus_tag="VBD11_001013"
     CDS             complement(10336..10878)
                     /locus_tag="VBD11_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6953975.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            complement(10996..12144)
                     /gene="nagA"
                     /locus_tag="VBD11_001014"
     CDS             complement(10996..12144)
                     /gene="nagA"
                     /locus_tag="VBD11_001014"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6953976.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(12422..13129)
                     /locus_tag="VBD11_001015"
     CDS             complement(12422..13129)
                     /locus_tag="VBD11_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6953977.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            13320..13631
                     /locus_tag="VBD11_001016"
     CDS             13320..13631
                     /locus_tag="VBD11_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6953978.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            13652..14335
                     /locus_tag="VBD11_001017"
     CDS             13652..14335
                     /locus_tag="VBD11_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953979.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYCHLPFINIPLWIYLIILGLSFSLHIIVKKY
                     LNKHYYIEF"
BASE COUNT         4607 a         3000 c         2179 g         4563 t
ORIGIN      
        1 taacttatcc ttataataaa cgattcgaca attaactttt tcttgttcaa agtagctatt
       61 aagattattg atatatttaa gcaaagttgc tcgacttaaa ccagtttcta ataaagctaa
      121 cttcagtgct attgcctgag gagagcctct taaaaaagaa agtagccgat attgaacacg
      181 cctttctttt tccattagag cttctattcg catagcccaa tcctcttttc ataaaggttg
      241 atagctgctt gatgtaacat ataagccatc tcagattctc tagctgtata aatgaaatct
      301 tgaaaagcca aatcaaaatc tgtcatgtca cacgatatag cttgaacaag ctgattggca
      361 gtagccaaac aattttcctt tgagattaat tgagccccca ataaatggcc cgttttttga
      421 ttagcaatca attttatcct taaatcatac tgttctttag ggaaataagt aacactaacg
      481 gtatcctcgt aaagaccagc ttccagctca gttaatccaa tgttagctct ataatagcca
      541 aagtgtttag atcctgtagc gcgtgttatt tttaattttg gaacagcatg tcccgataaa
      601 tggtatgcca acatttgtgc cgaacggatt gcttggttaa ttaagggcat ataataagca
      661 ctattaagac ctttaaaata cgaccaaatc aaatcgccaa tagcatagat attagggtca
      721 ctcgtttgga aataatcatt tacaacaaca ctattatcaa cacttaaatc aaccaaccca
      781 gttaaaagac gactatccgg acgaaaatta acagctaata tgacagtatc aactgtaaag
      841 gtgtctcctg ttagcgttcg caaacaaagt ttttcttctc gggttacagt aattttctca
      901 accttttgat tttggaaaaa atgtagtttt gattcagcca tcgcatcaag taaaggaagg
      961 gacatatcag gatctgtatg cctgaagtct ggtctttctt gagcttctaa caaagtgacc
     1021 gccttccctg attcatgtaa actataagca atatctaaac cgatgatacc tgccccaaca
     1081 actgctacct tatgactttt atctaatacc tgcttagcat tttgactagt tgcataagtt
     1141 tttgttgtta aaacaccttg cgcatcagcc ccatcaatat aagttgattc tgcttgggag
     1201 cccatggcac aaatcaattg atcataatag acagaagaac catctgataa cattaattcc
     1261 ctatcttctt tattaatttt ctcaactttt agaccaaata gacagcgtat attctgcaac
     1321 cgttgctctt cttctgtctg ccacatggcc tgatttaaac cactaatttc gtgacgataa
     1381 taccaattta agccatttgg aacataatct ggatgaattt ctttatcaat caaaacgata
     1441 tcccaattag gatttaattg gcgtaatttc tgcgcacaag tcataccagc aaaagaagct
     1501 cctaaaatga caattacttt atttacatat ttttctgtca taattactta ttataacaaa
     1561 agaagttatt tttgaaaacg gtaacaaaaa actgagacca taaagtcggt ctcagcatca
     1621 aaaatctctt acatcatagc aaatattaag gctgctaaaa tagctcctac cataggtcct
     1681 actatcggaa tccatgaata agtccaatca gaatcacctt tgttcttcat cggtaacaag
     1741 gcatgcaata aacgaggacc aaaatcacgt gcaggattaa tagcataacc tgttgttcca
     1801 ccgagcgata gaccaatccc aaccactaac ataccaacac tcatcgtacc aactcctgga
     1861 ggcatattgt attttccgat ggcaaaaata gtaagtacaa gaacaagtgt tcccaatact
     1921 tcacttaaaa aattggaaaa attatccttc aaagccggac cagttgcaaa agttccaagg
     1981 atatctgctt tattttctgc agcctcataa tggggtctaa attgcaaata aacaagaagt
     2041 gaacctagca ttgcaccaat aatctgtgca atcatataaa gaatagctgt tccccaagct
     2101 aaatcacctt tgatcgcaaa agctaatgac acagctggat tcaagtgggc agggctaata
     2161 ttgccagata ccaacgctgc aatagctaca gcaagccccc atccaaatgt gataacaatc
     2221 catccagaat tatgattctt cgttttaggc aaaacaacac ctgctacaac accattacca
     2281 agtaagacta agagagctgt tcctaagaat tctccaaaaa tatccattat tatctccttc
     2341 aattatcctt caaagctgcc aagtcattgt cttttagagt ctcatgtaat tcttcttcat
     2401 agaaagtttt gtcttgatca gaccattgat aatgtttagc catctcatca ataacaggct
     2461 gaatcaaaga atctaaatta tctctcatga aaagcatatg attcgtacgt cttaagaaga
     2521 aatccgttgg gctaagcgcc atttcataat ccatagcata gtgaagtgac aacgtttcta
     2581 ccaagctcag accttctgct tctgtaattt ggcgtgtcaa tgcaaataat tttggtgcat
     2641 ttgacccata aaggttagca atataacgtg catcctcaat acttaaacca cttagtgtgc
     2701 ccaattgagc ataagcttca atttcagaat caacattaga tgggttaatc tctcctcctg
     2761 aaacaggata ggttttagaa ttgattagtt taaacttgcg attatattct ttatccaaaa
     2821 tgttaataat agtttccatg gcaccttctg ccatttttcg ataatcagta attttaccac
     2881 cagcaagtgt taataggcca ttatcatcac gctcaaaact tgatcctcga gaaacagctg
     2941 atgggtccaa ttctttttca gaagtactag attctacatg agaaatagct ttttcaacat
     3001 cctcacgatg atttttatgt gcaatataat ctttaacgga atcaattaat tcttcaaaac
     3061 tttcatcgct tagttttcca ctattgcctc cattatagtc agaggcacta ttccctgata
     3121 gtagtggacg cagacctgcc catgaactct caatatcatc aatagtaagc tctgcttctg
     3181 ggaagcgttt gtttacaata ttaaggaggt agtcaacatc ttctttagtt accgtaggat
     3241 gttctaagtc tccatggtaa tcagtatctg tggttccaaa ataagtctta tcttcacgtg
     3301 gcaaaacaaa aatcatgcga ccatcattta gacctgtatc aacataaaca ggttgagaaa
     3361 catttaattt ttggcgatct acaactaagt gaactccctt agttggacgt aattgatgaa
     3421 tttgtttacc ttcattggag aagttgcgaa cggtgtcact ccaaggtcca gttgtattaa
     3481 taacaagtct agcttttata tcgataactt ggtcagtcaa caaatcacgc gcacgtactc
     3541 ctataatttg attgttgtca tcaaataaga agtcctcagc tttgacatga ctggcaatat
     3601 aagctccatc cctattagca cgtttaatat tttcaatcac taggcgtgca tcgttattac
     3661 ggaaatctaa atagacccct ccgcctagca aaccttcttt ttgtaaatca ggctcacgct
     3721 taagaacatc ttctgcgctt aaaactttat tggctgcggg tgtgtttgtc acacctgcta
     3781 ataaatcata taaatccata gccactttaa ggcgaaacat actaaaagta gaacccggct
     3841 catcgtaaac cggtaaaagc attggatctg gcttaggaat gtgtggtgca atttgctgca
     3901 cgactgcacg ctctgatact gtatcagaaa caacttctac atcaaattgc ttcaagtaac
     3961 gaagaccacc atggaccaat tttgttgagc gactacttgt cccttctgca aaatcttgca
     4021 tttcaataag tccagtatct aatccactag ctgctgcttg taaagcgaca ccagcgcctg
     4081 tgataccccc acctatgata agtaaatcca gagtacgatc ttgcattctt tgtaaggcta
     4141 aacgtcgtgt ttctcgtgaa aattccatct ctacaccccc ttaatcctct tgagcaaata
     4201 cttgtgtcgc tttgacagct tttctccacc ctttatacag tttttctttt cgagactcat
     4261 tcattgttgc ttggaaaagt tgtcctgttg cgttcagttc ttttagttca tccatacttt
     4321 cccaatagcc aactgaaagt ccggctaaga aagctgcacc caaggcagta gtttcaaggt
     4381 tcttcgcacg agcaatatca atccctaaaa tatccgcttg gaattgcatt aataaattat
     4441 tcattgcagc tccaccatct acacgaagtt gttggatatc aattcctgaa tcaacttgca
     4501 tagtgtcaat aacgtctcgt acttgataag caattgactg aagtgtagct ttaacaaaat
     4561 cttctttact tgtcccacga gttaatccaa atactgaccc tctagcattt gaatcccaat
     4621 acggtgcacc caaacctgta aaagctggaa ccacataaac ctcatcgtca cttgttgatg
     4681 attgagcaag gccttctgac tcacttgacg tttcaatcat tctaagacca tcacgtaacc
     4741 attgaatagc gcttcctgca atgaagattg atccttcaag tgcataatga accttaccat
     4801 taatgccgta accaatcgtt gttaatagat tattttgaga cagttgcatc tcttcgcccg
     4861 tattcatgat aatgaaggaa cctgtaccat aagtgttttt taccatacca ggttcaaagg
     4921 ccaactgtcc aaataatgct gcctgttggt caccagccat accagaaatt ggcacctctc
     4981 caccataaaa atggaatgga gtagttttcc cataaacttc agaattagat tttacttctg
     5041 gtaacattgc tttaggaata ttcagtaact ctaaaatttc atcatcccat ttaagttctt
     5101 taatattata aagcatcgta cgagcagcat ttgaatagtc tgtaacatgg acgagaccgt
     5161 cagttaactt ccaaactaac caagtatcaa ttgttccaaa aagcaactct cctttttcag
     5221 cacgttcctg tgctccagga acatggtcta aaatccaacg aacttttgtt gccgagaagt
     5281 aagcatcaat gacaagacca gttttttcat gaatcatatt agtatgacct tcttgcttca
     5341 attgatcagc aatcggggct gtttgtcgcg attgccatac aatagcatta taaattggaa
     5401 gacctgtttt tttatcccaa acaacagttg tttcacgttg attggtaata ccaatagcct
     5461 ctatctgtcc aggtttgatg cttgattcaa taaaagctcc agcaatgaca gattgaacag
     5521 aattccaaat ctgattagca ttatgctcta cccagcctgc ttgaggaaaa atttggggaa
     5581 attctttttg gctactcgca attttttcac cttttttatt aaaaataatt gctcgagaac
     5641 ttgttgttcc ctggtcaatc gccataacgt atttttcttc tgaagacata aacttcctcc
     5701 ttaaaagtaa gcgttttctt tttgtaattc tattatacct aaatcagtct atcctgcact
     5761 atcaaaaatt taatatgccc ctaaaatttt tgataagata tcatcaataa caccaaatta
     5821 gccaaataat agctaaatca ataatcattc cgatatacca aactagtcta aaataccatt
     5881 tagtcgtttt atgtcgaaaa acttctccac ctaggaatgc accaaatccc cctaataata
     5941 aactctctat caacaatgtt ttctcactaa tacgccatgc ccctctaatt gcacgccttt
     6001 tatcaatagc ataggttgca aatactaaaa tattccataa cagtaatgct aatagtaatt
     6061 caatcattat tttttccttc ctaataaaaa aggcgagaca aagattatct tttgccccac
     6121 ccaagtgttg actttaattt aaaattgtct caccattaag aatttggatc atccaaacta
     6181 cgaccatgta gtcctttttc tctctggact tgacgaagtt tttccggggt aacatcattg
     6241 ccctcatcat ccactaattt aattccttca acgtggtgac ggactgatcg gcggaaacct
     6301 tcaatatact cttcacggag ctttgcttgt tcaaccttct cttctccggt caatccaact
     6361 gtttttttct tttttgaaag ctcgttaata cgagcaattt ttttaggatc cataaaatgc
     6421 ctccttattt tgtgtttaat aagttaaaag cagcgactgt tcttgccttt gcaacaaggc
     6481 tattgagtaa agccagacga ttttgtttca ttttttcatc atctgtcatt accattgtat
     6541 tatcaaagaa atcattaata attggactaa gtgcgaaaag cttatctaag ttgtcatgca
     6601 tatcctcagt cagttcaagt gacaaaatag cttgatatag cgccttctcc tgattatttt
     6661 caaacaatga agagtccacc gatacagaat gagtaacttt ttcagctaaa ttaaatgcac
     6721 gtgacaaact ttcaacggaa actttatatt gagcatcctt gctcttttgc accaatgctt
     6781 ggctagcttc tagaataagg ctaacaatat aagtgtctga ttctaaaacg gcttctcgaa
     6841 tatctttagg gaccttagag ccaatcatct tttcaatacg agctgaaata aaggccatga
     6901 cctctttttg attagcataa tctaaactag caaacgatag accataaagg ttagttacta
     6961 attcatcaag tgggatatcc caaccaaaag cttccaaaat acggacaatc ccctgagtag
     7021 ctcgacgaag agcatatgga tcatttgaac cacttggaat caaaccaact gagaagaatg
     7081 aaagtaaggt gtcaaattta tcagctaacg ctagaattgc accaattctg gtttctggaa
     7141 gctctccatc ggccgatgtc ggcatataat gttcacgtat cgctgctgct actgcgggct
     7201 gttcccctgc aagtaaggca tatttctcac ccataatacc ttgtaattca tcaaactcat
     7261 caaccattcc agttaagagg tcaaatttat aaatacttgc agctcgaagc acagctaatt
     7321 tttcttcatc agaaagacct gccttatcag ccaaatgttg tgaaataact tttacacgat
     7381 ccatatgttc ataaagtgaa ccaatttttt catggaaagt tacttgtttt aatttctcaa
     7441 ctaaatctgc aatattaagt ttctgatctt cttgccagaa gaattcacca tcttcaagac
     7501 gagccactaa aactttttca ttgcctttta taacattttc gatatgctca gcatttccat
     7561 tacgcacaga aataaagttt ggtaacaatt ttccatccct gtcgcgaaca acaaaatagc
     7621 gttgatgatt tttcattgaa gtgactaaaa cttcttcagg tacatcaagg tatttctcgt
     7681 caaatgaacc caagaatgct gtcggatact caactaaatt aagcacttca tttagtaaat
     7741 cttcgtcaat ttcaactgaa atatttttct tttcttcgat agcatgaatt tgattaacaa
     7801 tcatttgctg acgttctttt gcatcagcaa taacaaattg ttgtcgcaag tcatcttcat
     7861 acgaactggc agatgaaatt tcagtgtcag aacccaagaa acggtgtcct cgactaatac
     7921 gccctgaatg aatatcaagg aagtctagct ctaaggcttg atcatctaat aataccacta
     7981 aagtgtgaac tggacgaata tattcaaaac tattatttgc ccaatgcatg ttcactggga
     8041 aagtcaattc tgttaatacc tctgtcacac tcgctaaaac atcaatcgca ctttttccag
     8101 tttcatgctt agtgacataa acatattctt ctcccttaac ttcacgaaat tcgatatcat
     8161 caacacttaa accttttccg cgaacaaaac cttgggctgc tttagaaaaa ttcccttctg
     8221 catccaaagc aattttctta gaaggaccct tgaaatcttc tgttaaatct gtctgctggt
     8281 ctgccaatcc tttaacacga acagccaaac gacgtggagt tgaaaaagtt tgaacagttt
     8341 caaatgataa acgatgatcc tctaaaaact tgaccatttt ttggcccaat tgcttctcac
     8401 tcggtgtcac aacataagct ggcaactctt caagtccaag ctccaataat aaatctttag
     8461 tcatttccta ctccttacta taaaacgctt caaatgcttc tcttaaatcc tgattcatct
     8521 tatcaagaac aacttgccta tctaaaatat cggtaccttc tgcacgaaca acacagaaat
     8581 catcaaaagc cataaaatct ttaaagatag cttttaaata atgaacagga aattcatatg
     8641 gactatattg tcctttagaa taaatagaac ccgcagattc taacataagc agacgatagt
     8701 catctgtcat taatccgacc gaaccatttt cagtgtactt gaaagtctca cctgcaataa
     8761 aaatattatc aatataagtt tttgctctag cagtcacatt gtaattgtgc attggagaaa
     8821 caaagacaat acggtgatgt gacttaaact gttcaagtaa atctttagag attttagctt
     8881 gaacaatctc atcggctgtt aattccaaac cctttctttg tttagaccaa atcgaaagta
     8941 gtacttcttt cgtaatttct ggcaaaacac aatcatataa atttaaaata gataactgat
     9001 ggttagggaa atgctcatta taaagttcaa taaacttttc ttgtagaata gttgtaaatg
     9061 agtacggatt cgaaaaatca ggatgactat taacaatgag tgtgttcatt agtcttcctc
     9121 agcaagtaat ttgatacgag tttcctcatc caaaagtggg aaaccaagtt ttttacgttc
     9181 ggcaacaaag gttttagcaa caacacgtgc taaattccga atacgtgcaa tatagcctgc
     9241 acgctcagta acagatactg ctccacgtgc atctaataaa ttaaaggtgt gtgaacattt
     9301 aaggacgtag tcataagctg gatgaacaag tccttcttct aaagcacgtt ttgcttctct
     9361 ttcaaatttc tcaaagtttt caagtaacat tacttggtct gatatctcaa atgaatattt
     9421 tgaatgctca tactctggct gagtaaagat ttcaccatat ttaacacctg gtgcccactc
     9481 aatatcataa acagaatcta cctcttgaat ataagaggct aaacgctcta gtccataagt
     9541 aacttcagaa gttacaggac ctgtttgaag tccacctact tgttggaaat aagtaaattg
     9601 tgttatttcc attccatcaa gccaaacttc ccaacctaat ccagctgaac ctgtagatgg
     9661 attttcccag ttatcttcaa caaaacggat atcatgctca agtggattga tacctagtaa
     9721 ttccaaagac ttcaaataaa gttcttgaat atttgacggc gaaggcttca ttaccacttg
     9781 aaattgatga tgttggtaaa gacggtttgg attttcacca tagcggccat cagctggacg
     9841 acgtgatggt tcaacgtatg cagcattcca aggctcaggg cctatagcac gtaagaaagt
     9901 ataagggctc attgtccctg ctcctttctc attatcataa gcttgcatta acatacagcc
     9961 ttggtcattc caaaactgtt gtaaagttag aataatttct tgaaaagtta atttcttaga
    10021 catcattttc cttctttcat actttattac agctgttggg aaggtgacaa caattgactt
    10081 aaaacggaca aatgataaaa accacgtcct acaccaccta tgcccaacag cacaggggcg
    10141 tcaaagacgc ggttccaccc taatttatta cttcattttt gattgtactt ttactcacta
    10201 aaaagtgcca atattcatcc tattctaccc aactttcact accttgagct cgctaaaaaa
    10261 acagctgatg aacacctctt cttactgaga cagtataaca aaaaatgact tttatgtcta
    10321 ctatttatct tattcttaag ataccctaga ctttttgatt ccatttggaa taatgatcgt
    10381 ttttaataat aagttttctt cacgaattct taaataaata acaaaagcat aaattggcaa
    10441 gacaaataat gctgtatact tagcatgaca caataaagtt acacctaaaa gttcaggagc
    10501 aatatttaag aaatagttag gatgcttaac aaccctaaat agccagtgat ctacaaattg
    10561 atgattttta tctaccatta atttcactgt ccaaatacga ccaagtaaac gtgttactgt
    10621 atgtaacata aacacagaaa aaatcatcaa gctaagcccg attacgctaa caaaattaaa
    10681 acttgctttt gttagaacag cctcagttac agatgaaaaa taaatgataa tatgaagaac
    10741 tgtgattgct tttgaaacac ctacaccata ttctttagca ccattttttc ttaaggcctt
    10801 ttcattagct atagatagct ttaaataagc taaacgaatg ataaacatag cggccattaa
    10861 cccaataata attgacattg taaactccta ttgttttgta ttatattaca acattataag
    10921 aaaaatagtt ttattgtcaa actatatttc attaaaaatt aataaattaa aaagaactag
    10981 taaaaactag ttctttcaag attgccaacg tttttctcca tcaaggtatg ttgcaactaa
    11041 atctagatgt ttatctaata caataaaatc cgctgcatag ccttctttta tctgtccaca
    11101 gacattatca atgcctacag acactgctgg aacataactc gccatacgca ctgcctcttc
    11161 cggagtagca attccccatt ctacaacatt ttttaatcca tctttgagct taagaataga
    11221 accagctaaa ttccctccat ctttcaaacg cgcagttcca ttagacacaa ccacgggcaa
    11281 ttcccctaac atataatctc catcaggggc acctccagct gccatacaat ctgttattaa
    11341 tgccacatga ttatggcctt tttgtgtcat taaaatgtca caagcaacag gatcaacgtg
    11401 atgaccatcg caaatgagtt cagcataagt attaggtaaa ttataaaccg caccaaccat
    11461 acctggttct cggtgtgtca aaccacgcat accattatac gcatgtaccc aaactgaggc
    11521 acccgccttc actgcctttt tagcttcttt atatgtccca ttagaatgac ctaaagctac
    11581 cgtaacacct tgctttgtga ttgctgagac aaattcctca actccctcac gttctggtgc
    11641 caaagcaatt tttgtaatca agccttttgc agccttctgc cattgagcaa actcttccaa
    11701 gtttggattt ctcatataaa tcggattttg agctccttta tattcctcag taaaatatgg
    11761 tccctcaaaa tagatgcctt gaatttttgc tccttgttct ttgcccgcta ctgccgcaac
    11821 tgctttagac gcctcttcta gtcgctttgt atctgaagtt agggttgtcg gcaagaaaga
    11881 tgtgactcca gtagatagta agccagcact cattcgcaaa attccttctg aatcacaatc
    11941 cataacatca gcgccagcaa aaccatgaat atgtgtatca actaaaccag gtgctatttg
    12001 gtaacctgaa taatctaaaa tctcctcttg tccagaaata ttttctatcc atttaccaaa
    12061 gtgattatct tttatttcta aataaccgtt ttcctttaca tgatcagcat aaaagaatcg
    12121 gtcagccttg atgtacttcg tcatcttctt ccccccattc actctcatta taacgcttta
    12181 aggtattttt gtaaatggta tatacctatt ttttaaaaag tcatggttta ttctaagaaa
    12241 gaactataca cttgttataa accacttcac ttggttgtac ttttaaggtt ttaattgctg
    12301 ttgctcataa ttcttttcct tctccaaaac tatcaattgc taattctctt atagtttttg
    12361 atgagaatgg ctaattaaaa agcatagcaa aaaagtctag taaaactaga ctttcaggat
    12421 tttactgagc aacacccgtc ccattagtag caggtacagt accagttcct ggagtcggaa
    12481 cagtattgtt aacccctgga gtaactggtg tattattcgt ttgcggttgc tcataagttg
    12541 gtgctacatc ctgattaacg taactagaag tatcctgact agcaggatta gctgtccctt
    12601 gttctgtagt ttcagctact gaactagagg gagtttgatt cccaatcaaa ccatattgtg
    12661 tcttaacagt atcaatacgt ttttgtaaag cttctttaga ttctttgaca gttaactttt
    12721 tgactgctgc ttcagctttt aacacatttt cttctgaagg ggcttgttct agaatttcta
    12781 ctaaatctgt agcatctttg gtagctttct tatctgcttc gtctttaaga tacttatctt
    12841 ttgctttctt aaaacgtttt tgcaaagctt cttttttaga agaattggac aatttattta
    12901 aagcaagttc gacatccttc aataattttt ctgaaggttt cttctcaaat ttatctaaca
    12961 tatcttgtgc ctcagttaat tgagaatcac tgacatggtt aacactgctt tctacagtat
    13021 ccatttgagt agtgccatga tctttaaata agaaaaacgt aacagaagca aaaattaata
    13081 aaataatact agcaataagt gctaaaagtt cttttttatc tattttcata ctcactccta
    13141 aagtttcttc acattattat aacataaact aaaggacata aacaaaccta ttttagaaga
    13201 atcatcgtct atcactctta ttttctaccg gtaacttagt tatatttgac atctcaacta
    13261 ctttgtatta caatgtagtt aaccagtaac ttgtattaca atatagataa ggatataata
    13321 tgattccatc gcaaatgcta aaaggattac ttgaaggagc tatcctaaaa atcatacagc
    13381 aagaggagac ttatgcttat gaaatctcaa aaaaattaaa ttcttatggt tttggggaaa
    13441 tttccgaagg gacaatctac cccattatct taaggctaca aagcaataaa tatataaaag
    13501 gacaacaaaa gccttcccct caaggaccta aacgtaaata ttatacgtta accaatgaag
    13561 gcgtggagca attagcccaa tttaaacaga attgggagtc acttagtaaa gcattaaaca
    13621 aactactcta aaaattggag aggaaactag catggataaa ctgaaaaaat tacaaaaaga
    13681 aaatacgcta ttagaaggaa gaattgataa ctctaataac caaacctata cagatatgat
    13741 tgtctatttg cgaggtgcta gcattagccc ctaccaccag gagctcattc gaaatgatat
    13801 tgtcaatatg ttattagaag cacaagagcg acaagcaagt ttagttagtg tatttggaga
    13861 agataggcac gactttataa accaagttat taaatctaca cctaaaatct ctaagaaaga
    13921 agaaacatta caacgttggg atttagccat tttactacta actatccaaa tgattatctt
    13981 tttaggagga tatctcatta ctgaagcact tcaacagtca gtaccagatc taatccctat
    14041 tactctatta gatgttcttt ttgcaatctt tatcagtatt attgctgtca agataactga
    14101 taccataata tatgctacat ataacttcga taaatcgaaa gaaaagaaat atttctttcg
    14161 atatatgttt ctaattcttt cgcttatcat agcttatatc cttataggaa aatattgtca
    14221 tcttcctttc attaatatcc ccctttggat ttatttaatt atactgggac ttagcttctc
    14281 tcttcatatc attgttaaaa aatacctgaa taaacattat tatattgaat tttaaaagag
    14341 gtataacta
//
LOCUS       DATROY010000043        13932 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.044, whole genome shotgun sequence.
ACCESSION   DATROY010000043 DATROY010000000
VERSION     DATROY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13932
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(115..4830)
                     /locus_tag="VBD11_001018"
     CDS             complement(115..4830)
                     /locus_tag="VBD11_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6953980.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISP
                     NDDQNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     QSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKIKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            5153..5845
                     /locus_tag="VBD11_001019"
     CDS             5153..5845
                     /locus_tag="VBD11_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6953981.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            5835..6158
                     /locus_tag="VBD11_001020"
     CDS             5835..6158
                     /locus_tag="VBD11_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6953982.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
     gene            6528..8765
                     /gene="metE"
                     /locus_tag="VBD11_001021"
     CDS             6528..8765
                     /gene="metE"
                     /locus_tag="VBD11_001021"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6953983.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            8810..10654
                     /locus_tag="VBD11_001022"
     CDS             8810..10654
                     /locus_tag="VBD11_001022"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6953984.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            11001..11708
                     /locus_tag="VBD11_001023"
     CDS             11001..11708
                     /locus_tag="VBD11_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6953985.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(11873..12958)
                     /locus_tag="VBD11_001024"
     CDS             complement(11873..12958)
                     /locus_tag="VBD11_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6953986.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(13159..13698)
                     /locus_tag="VBD11_001025"
     CDS             complement(13159..13698)
                     /locus_tag="VBD11_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6953987.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         4196 a         2311 c         2638 g         4787 t
ORIGIN      
        1 agatattgca gagaaaaggt ctttaggaac atcattttaa gctcctcaaa aaggcaccag
       61 tcactaacta ttaatagcta gtaattgatg ccttgctcta ttttttaaag tattttattc
      121 tcttttgata gactttagtt tgattgttgc cagacctagc atagagagac taaatagcaa
      181 tattgtatag gttattttag gagtgctttc gcctgtttta ggaagcttgt ttgtctttat
      241 cttttgatta tcggtttggc tagtcacccc taagacacgc ttctgagcaa cctcactttc
      301 tttagtcatg ccttttttga atatcaacgc ctggggctga tttggcggat tgataggtgt
      361 ggtattgaga gagtcattat cattgagtga ttgactataa ttctctgcgg aagaaactct
      421 atcgcgacca tcaagttctt gttcggttgc ttttatttgg tttaataact cttcaacttg
      481 agcttgtgta gcttgtctat tggaaagcag caattctgct ttcttgataa ggttattata
      541 attttcttta gcaaattgac tggcgttctg atatttagat gatgtttgtt tcagaacatt
      601 tgctttaatt atcatatggt gtaaaccaga gtaatcagtt tcatgaccat ttaattgatt
      661 tttagcaata tcaagatttt ctaaggcttt gtctacttct gattgagtga cgttaggttt
      721 ttgtagaaga tttttcgcca attggacttg attgatataa gttgattggc ttaatggact
      781 agcattaaca aattgatagt ttcccttgat ttctgtttca gctaaaatag cttttgccag
      841 taaagaagta tctgtttctt taccgttaag agcttggcga ctttctctca aagcagcaat
      901 agcattatca atacttgttt gttccactct atttttaatt aaagtttgtg ccttttcaag
      961 attagctagg taattatttt ttaagtgagt tccagcatta taaaacacag gttgggaaat
     1021 aatgtcagtt tgttctgcta gggcattaat tagaggagct ttattaatca gcgtcaattt
     1081 tgttagattt acttgacctt cttttacgga tacaagtaaa tcatctaagt tctctaaagt
     1141 gctataccca ctaggcagcg ttacgtttaa acgatagtct cctacaggta tgtttttacc
     1201 atagtcagta ggagaataag ttgcttttgg tagatcaaca acagtatttg tcttagtaac
     1261 caactggact gttgctcctt ttggtaaaag cttgtcaaat tcaacgagta aggctgcttt
     1321 cttgagtaag ttaactttaa ataagacgtc tttaaggcta tctttttcag aaatagttac
     1381 agtgacactt tggggactga ttagatttgc tcgttcctca tcgtataaga ataggtcaaa
     1441 cgtataggta ccaaaaggca atttcagtaa attgaggtct ttcccgtgat gttgcttttt
     1501 gatgacatta cctttgtcat ctttgacaga gtaagagaaa tcaatatcga cattactgtt
     1561 aagctcaggg ctaaacactt tgacgtttac taaaccattt ttattgccaa tattgatcaa
     1621 atcttcgagt tttgctgaca ctagattgcc tgcataatct tcaacagtgt agtagacatg
     1681 agagaaatca ttaacctctt taggtagaat aaagctacca tccttttctt gtttgataaa
     1741 tactttgtta tcttcaacaa ggataccgtc ttctcctttt tgacgtaaga cgctattata
     1801 atgaccatct ttatcttttt ttaagtaaaa aacttgttct ctaaagatac cagattcccc
     1861 gtgttctact gcaggacgag ctttgaagat acgtctatct ttgtcataag ttgctgttgt
     1921 tagtgtagga gcttgtctat ccaaagtgat atcaaatacc atttgttgtg gcttagagcc
     1981 agggacatca gagtagtatg ttaaaacgta tttgtatttt ccatcttcta aaggaatacc
     2041 atcagtagtg gttcctttcc attctgtgtc atataaaaat gtagattttg gattttcagt
     2101 atttccgcta tagtaatttt tatctcctgc ttggggagca cttacccata gtggtttttg
     2161 taaattaaca tcatctgcac gatagacttt ggctcttaaa ttattgaaat tacgtaagaa
     2221 aacacctttc agtgcaacgg catcttgatt ttggtcatca ttaggtgaaa tggctagatg
     2281 aggctggcct ttttgctcta gttgtaagat ccattttcca tctatagact tatagcttcc
     2341 caaggctttc aattggatag gactagtacc gtctgttgag tagataggct ctgaacttgt
     2401 agtcattaag gcagtataat cttctgaacc aggaacttcg tttgtttgtt tcggttggaa
     2461 ataaaatccc ttttctttgt tagcaacaag cttataaatg gatttttcta aaacttctaa
     2521 gttttggaat tctcccttaa atccaacata aggaatacta agaacttccc caccatcaac
     2581 agggtctgta aaacgtacgt agccttctag gaaatagccg tttggcatta ctttttttaa
     2641 catgtcatgg tatttcgaaa catctatatc aattgtaatc gttttagttt gccctggttc
     2701 aatccgtatc gttttccctg taaatgtacc aagttgttga ggagccaatg tgacaaagcc
     2761 atctttaact tgatcagtat ttaaataggt tgtatagtgt aaatcttttg caactttatt
     2821 gatattatga acagtaacat caaaggaaat tttatcttta atatttccta atgtaacgct
     2881 accatagttg ttttccccac cagttaggta taatcctgtc tgaactgctg ctgcgacatc
     2941 gataatacct gctccttgtt ggcgtggtga ggtgtaagcg cctgtatctt tgtttaggtg
     3001 tgctttagca gtactcataa gaaggtattt gacagttctt tcaatgtcac cttttttaag
     3061 ttctggatgt tctttcaata agtattgttt aactagagca gtagcaccag cgacatgggg
     3121 agaagccata cttgtcccac tcatcatatc atattcatta tcattgatag cggcgtaaat
     3181 agagcctcca ggagcagata agtcaggttt taattgtccg tctgctgtta atccccagct
     3241 tgaaaagtca gataggacac ctgcattagg gttggcttgt ttatttttga tattgttaaa
     3301 tacaatttta tagttgtttt tagctaaaac atctccaaac tctttttgag taaaaatggc
     3361 aggaatagcg cttgcttcag gatctaaact cattgttaga ttagcttctc cagctttgtt
     3421 attaaagata atagctccca cagcaccgtg attaatagcg ttgacgactt tttttgtaaa
     3481 agtaatatct ccacgttcaa ttaaggcgat tttaccattt aaagttttgt ccttataatc
     3541 attgtcgttt ccttttccta cgaaaacata atcatattgc ttccctactt caaaggtctt
     3601 atcactaact ttaggatctg cataagcagc taatccgttg ttgagatttt tgttgttttc
     3661 taatccgata atgttgaaga ctttattaac cagtgttgta ttattatatg atgctacaga
     3721 aatggcctca cgagctgttg atggactacc aactaaacca taatcaggat aaagagcaga
     3781 aggctttgat gctccactcc caaatgtacc gtcgttacct gctgctataa caacagaaac
     3841 gccagcgagt ctagccatct ctaaagcttt tataagtcgg tcatcggcat taactaaaga
     3901 gccattagct ccacctaaac ttaaattaat gctatcagca ccgagtttaa cggcatcttc
     3961 aatagcttta acataaaggg ctggtccagt tcctcttttt tcatcagaga agactctcat
     4021 aaacattact tgtgcttcag gagcaacacc atagattaat tcatttgtat ctttcttgct
     4081 aggattagct gttgcgatac tggttacgtg cataccatga gaagtgcttt tcacctcttt
     4141 tagctctgta ttgacatcaa catagttgtg accaaagatt actttgttgt tataccattt
     4201 tccatagttt ataccagctt ttgctttagc agcattcatt tgctgttcgt tttggtattt
     4261 tgcttttgtc gaatcattta attggagagc atcgtgattg gtatctagtc ctgagtcaat
     4321 aattgctaca acagttccct ctcctttgta gcctgtgttc catactttgg ggacagtgat
     4381 aattttatta ctatcaatgt tttgaggggt ttctttagaa atagctggtg taggtttgac
     4441 tgctaaattt tgtttggtta aaggagtttt agaaacttct tgagtgcttt caaccggttg
     4501 atttgcggct ggggaaaatg tctcggtggc ttgagagtga ggctcagtta ctggttgtgt
     4561 ttctgttgga ctattattat tagcctttgt aacaagattg tcttgagctg ttgacgcaag
     4621 ggctgatgac gtgttgttag taggtgtctc atttgctatg ctcgttggac tgtcttttac
     4681 ttgctgttca actgcaatat cagatggctt atttatgaca gaatcagcag ctacatttgt
     4741 gataccgcct aaaaaaaata gggttcccaa aagtacagat acggcaccta acttatattt
     4801 ccggattgaa aaacgctgtt ttgtgttcat aaaaatctcc ttttcttgta acataataat
     4861 tacaaaaact attaaaacat aaaaaatcaa aattttctac aattttctga attttcatta
     4921 caaattattt tgtggtcaat ttaagaccgt acctaaagca ttttttaaaa atcattctat
     4981 gaaaataata atattgtcca actatatggc tacaatatca aaggtttata gaataattac
     5041 aggatattta ttagtctaat tgctggcaaa gctaaataat cattcctctt ctttcttagt
     5101 aatcaccgtt gtgctataat aactgaatat actgaaaatt tagggggata tgatggaaaa
     5161 aggatttaaa gagggagtga aagatgcctt gcccacagct ttagggtata tctcaatagg
     5221 cttggcgttt gggatagttg cttcagcatc agatttatct gcaatagaag ttggtcttat
     5281 gagtgccttg gtatatgggg gatctgctca gtttgctatg tgtgcgctat tattagcaaa
     5341 agcagacttg atgactatta cgatgacagt atttttagtt aatttgagaa atatgttaat
     5401 gagtttacac gcgactacta tcttcaagtc tgctcaccta atgaatcaat tagccatagg
     5461 aactctaata acagatgaga gctacggcgt tttacttgga gaagcattgc atcacaaagt
     5521 cgtatcgcct agttggatgc atggtaataa tgtgatgagt tatttgacat gggtaatctc
     5581 aacgataata ggaactttac tggggagtac tattcctaat ccagaaatgt ttggtttaga
     5641 ctttgcatta gtcgccatgt ttataggtct ctttgtattt cagttgtttg ggatgttatc
     5701 tgatggaaag cgacttgttg tatatgttct tgcttctgta ggtttaagtt acttcttatt
     5761 atccactttt ttgtcaggag ccttatcagt attgcttgcg actgttgttg gatgtagtgt
     5821 aggagttgtt ttagatgata aatagtaccg tttttcttgc tattctttta ggttttgttg
     5881 tttcttgggt gccaagagtc gctccattca tatttgtaaa atacaaaggg ctaccaaata
     5941 gtcttgtgaa atttttaaaa tttcttcctc tttctatttt atttgcccta acattctcta
     6001 gctttttttc agttaaatta ggacaattgc caacactaaa atggttagat ttattggctg
     6061 gcataccaac tctagttgtc actattcgat atcgaaattt attttacact gttcttactg
     6121 gtatcgttac tatggcttta ttgagattac tattttaacg tgtaggaatg gaagattaat
     6181 aaaaacacaa aaaacgcatc tatttctaga tacgtttttt gtgtttttgt ctagacagga
     6241 acttttgata gccgatgtgt aatagtttga ctaattgtta ataatatcaa cagatttttc
     6301 tgtatcttga taatatgagt tgattgtaag attgcttttt aaagatatct acatgctgct
     6361 attttaaaat ttataaaaag gtccatagtg gtttatatag tttaaaacta tataaattgc
     6421 taaaaatata aaatattaaa agctattgat attatagttt tcatggacta aaataattca
     6481 taaaactaaa atggttagtt attagctata acttgatgga ggaaattatg gtaaaagttt
     6541 caaatttagg gtatccacgt cttggtgaac agcgcgaatg gaagcaagcg atcgaagctt
     6601 tctgggcagg gaatcttgaa caaaaagatt tagaaaaaca actaaaacaa ttacgtatca
     6661 atcatttaaa gaaacaaaaa gaggcaggta ttgaccttat tccagtgggg gatttttctt
     6721 gttatgatca tgttttggat ttgtcatttc aattcaatgt aatcccaaag cgtttcgatg
     6781 agtatgagag gaatttagac ctttattttg ctattgcaag aggtgacaaa gataatgtcg
     6841 catcatctat gaaaaagtgg tttaatacca actaccacta catagtccca gaatgggagg
     6901 ttgagactaa acctcacttg cagaataatt acttacttga tctttatcta gaagctaggg
     6961 aagtagttgg tgataaagca aagccggtta tcactggtcc aataacctat gtttccttat
     7021 catcaggaat tgtcgacttt gaagcgactg ttcagcggtt attaccactt tataagcagg
     7081 tctttcaaga tctgatagat gcaggcgcca cctatattca gattgatgag ccgatatttg
     7141 taactgatga aggtgaactt ttagtagata tagctaagtc tgtctatgat ttttttgcaa
     7201 gagaagtacc acaagcccac ttcatctttc aaacctactt tgaatcagca gtctgtttag
     7261 ataaactctc taagctgcca gtaacgggat ttggccttga ttttatacat ggtagggcgg
     7321 aaaatgtagc tgctgttaag caaggtctat tccgcgaaaa agaattattt gcaggaattg
     7381 ttaatggtcg aaatatctgg gcagtaaatt tagaagaaac gttggcttta ttggaagaga
     7441 taggtccctt tgttaaacgt ttgacccttc aaccttcttc aagtctttta catgtaccgg
     7501 tgacgactaa atacgaaaca catttagacc ctgtgttaaa gaatggctta tcatttgctg
     7561 atgaaaaact aaaggaatta gaactattag cgagtgcttt tgatggtaat aaaacaaagg
     7621 gatatcacga agctttatct cgtttttcag ctcttcaagc tgctgatttt cgtcatgtag
     7681 cgttggaatc attagcagaa gtaaggcttg aacgaagtcc gtataaatta cgccaagctt
     7741 tgcaagctga aaaattacag ttaccgattt taccaacaac aactattggg tcctttcctc
     7801 aatcacctga aattaggaag aaacgccttg cttggaaaag agaaaatcta tctgactcag
     7861 attataaaga tttcataaaa actgaaatta gaagatggat tgctattcaa gaagatcttg
     7921 atcttgatgt gttagtacat ggcgaatttg agcgcgtgga tatggttgaa ttttttggtc
     7981 aaaagttagc tggttttacg acaaccaaat taggctgggt acagtcttat ggttcaaggg
     8041 cggtcaaacc acctatcatt tatggtgata tcaaacatat tcaaccctta agccttgaag
     8101 aaacggttta tgcccaaagc ttgactaaga aacctgttaa aggcatgttg acaggtccta
     8161 ttactataac gaactggtca tttgagcgag atgatatcag ccgatctgat ctttttaatc
     8221 aaattgcttt ggctataaaa gatgagattc aacttttgga acaatcaggt attgctatta
     8281 tacaagtgga tgaagcagcc cttcgagaag gcttaccctt acgccagcaa aagcaacagg
     8341 cttacttaga tgatgctgtt gcggccttta aaattgcaac ttcatctgtg aaagatgaga
     8401 cacaaattca tacacatatg tgttattcaa aatttgacga aattattgat tctatccgtt
     8461 cactagatgc agatgttatt tctattgaaa cgagtagaag tcatggggac atcattgaaa
     8521 gttttgaaac agcagtttat cctctaggaa ttggtctggg tgtttatgat attcattctc
     8581 ctcgcatacc tactaaggaa gaaattattg tcaatattca acgatcacta aaatgtctat
     8641 caaaagagca attttgggta aaccctgact gtggcttaaa aacacgtcgt gaagcagaaa
     8701 caattgctgc cttggaggtt cttgtttcag ctaccaaaga ggttcgtcag caattagata
     8761 attaataggt taatcagtaa aggattgcca tgagggagga gtaaagaaga tgtccaagtt
     8821 tttagagaaa ttaaaaacag atattctagt agctgacggt gcaatgggaa ctctccttta
     8881 tacttatggg ctagatactt gtcatgaatc ctacaatgta acacatccag aaaaggttct
     8941 agccattcat caagcttata ttgaagcggg tgcagatgtt attcagacga atacctatgg
     9001 ggctcaacgt catcgattaa agaactacgg tttggaagat caggttgtct ctatcaatca
     9061 agcagcagtt aatatagctc atcaagcgac tttgggtaaa gaaacattta ttttaggtac
     9121 tgttggaggt tttcgttctc aaagacaatg cgatttaaca ttggataata ttgtagaaga
     9181 aactttggaa caagttgagg ctttattggt gacaggacag ctagacggat tattgtttga
     9241 aacttattat gatattgagg agataacgac agttttaaaa attgtgaggg agatgactga
     9301 tctaccaatt attactaata tttctcttca tgaagcaggg gtaacatcaa atggaaagcc
     9361 tattgtagag gcgctcagcc aattagttat gctaggtgca gatgtgattg gcttaaattg
     9421 tcaccttgga ccctatcata tgatccaatc attgaagcaa gttcctttat ttgcccagtc
     9481 ttatttgtca gtttatccta atgcaggtca gttatcttta gatggtgaga atagtcaata
     9541 ccagtttagt cagaattcag agtattttgg aaaatctgca gaacttctcg tagcagaagg
     9601 agttaggctt attggtggtt gttgtgggac aactccagat catattagag ctgtgaaacg
     9661 atctattaga ggattgaaac ctattgaacg aaaggttgtt actcctataa tacctgtaaa
     9721 agattttgtt aggcgtatca gacgaacaga tactctagtg gataaggtaa aaaaagaagt
     9781 aacaattatc gcagaattag atcctccaaa acatttagat atcgttcagt ttcaaaaggc
     9841 tatacgagcg attgaccaaa aaggtattgc agcaattact ttagcagata attcactatc
     9901 taatacacgt atttgtaatt taagtatagc tagcttgctt aaagacgaaa ttagtacacc
     9961 ctttttacta catatagctt gtcgtgacca taatttaatt ggtttacagt ctagactgtt
    10021 gggcatggaa ttattaggtt ttaaccatat tttagccata acaggagatc cgactaaact
    10081 tggtgatttc ccaggagcga ctagtgttta tgatgtgact tcctttaaac tcctttctct
    10141 gattaaacaa cttaatcaag gtttatctta tagtggtgct agcttgagac gtccaacaga
    10201 ttttacagtt gctgctgcct ttaatcctaa tgtgaaaaac cttactcgaa ccgtaaaatt
    10261 gattgagaaa aaagtagctt ctggtgctga ttattttatg acacaaccaa tatttgatca
    10321 ctctgtgtta agggaattag ctgatcttac taaaacagtt gaacaaccct tttttatcgg
    10381 aattatgcca ataacaagtt ataataacgc tgtttttcta cacaatgaag ttcctggtat
    10441 taaactttct gagtcatttt tatctgctct tgaaaaggta aaggacgaca aagaagcttg
    10501 tttaactttg gccttaaacg agagtaagtc cttgattgat gaagccttga accattttaa
    10561 tggtatctat ttgattactc cctttttacg ctatgatctg actcttgagt taattgacta
    10621 tatacaaaaa aagcaagtaa gaaagtcctc ttaactttct tactttttag ttggtcgttt
    10681 attattataa taaaagttac gtaccaaaac agaggttaaa ctagattttt aagccacttt
    10741 ctatactaag ataaattagt taatttaagt tttctttaag tttatagact tagaataaag
    10801 cgttattttg atgggtagca taaaatcaag gcataatata gatgtcagcg atttttttag
    10861 actaacgaaa taacgatgta aagcttaata ggggtgtatc gaatactcac ttatatagta
    10921 ccaaaagcct atagcctgtt ggctagcttg accgtattca aatgttgaat aaatcttggt
    10981 tgagaaaggg aggtataaaa atggtatgtg tcgattttat tgatcaaaaa gtttatagtg
    11041 aagcagaagg tctatttgtt tccgatttag acttaggcat taaaggttta ataagagcac
    11101 gttatccttt cgctctggat agtgacttta tttcgtatgg gaatttaagt cattattgta
    11161 tgaattatct tgatgaaatt gttcagcgag cgaatcagaa aaatgagaag ataaaatata
    11221 atgtgactac cttgatgaaa gaagaagaga agcccttcaa cgttgaagag cgtttgaaca
    11281 agcaagcaac tatcggtcaa cgaattgctg atgatgttgc acgttttggc ggatcttgga
    11341 cttttattat cgtttttgta tccattatgg ctatttggat gttggttaat atcatgaagc
    11401 cttttggtat tcaatttgat ccttacccat ttatcctgtt aaacttagct ttatcaacga
    11461 ttgctgcgat ccaagcacca cttattatga tgagtcaaaa tcgtgctgct gattatgatc
    11521 gtttgcaagc tcgaaatgac tttaatgtca ataaaacctc tgagttagaa attagattat
    11581 tgcacgaaaa aattgaccat atggtacaac aagatcagtt tgaattgtta gagattcaaa
    11641 agttacagac agaaatgcta gtatcattgg gaaatcagtt agcacaattg aaacaactac
    11701 aaaaataatc ttttagcagt tatttaaaaa tactgataca atactaagaa tttaaattaa
    11761 tactgcaacc tcctataatg gagagcagta tttttacttt attaataaca aaaacccctt
    11821 agatgatgtg tctaagggat ttttgtatcg gaataatcga gttggtgaat tattaggcta
    11881 gtgtctgtcc gaaagcgtct gctgctaaaa tagcttgagc aaacattttg ggatcgaaag
    11941 gggtattttt gtattcatta gtagtcattg caatttcaac aatttttgga atatcttttt
    12001 ctgataaaga gacgtcagct agagttgttg gcaaacctag tgacttatta aaacgagcta
    12061 ctttttcaag ttcttctagt tcgttgaaat aagcatgtaa aactaacaca ccaaacgcaa
    12121 ctacaacgcc atgtaagaat tcaccttgtc tttgaatagc ggttgtacca tagtaaaatg
    12181 catgcgcgtg acaagaattg tagtagaaat caggttgatt aactaaatta gaagcatagc
    12241 cagttgagat taagatatca agcgcaattt cttcaactgc acgcgaagct aaattagctt
    12301 caacgtcttt tagaccttgt tcaccaaatt gataaaaagc ttcctttgaa gacagagcga
    12361 ctgcttgtcc taacactgca gtatgtggta gtttattggt cttagcctct aaggtagcac
    12421 gttctacttc aggggctttt gaaataccat ccccaatacc agcccaaaaa tatttgcttg
    12481 gtgcctgtgc aatgatccgt gtattgataa aaatatgaag tggagactcg ggatagccgt
    12541 atcttaagaa agaatggtca tcattatata caactgcaat ggcagtacct gcggagcaat
    12601 tagaacaaat agttgggata gtaaagctat ttttacctaa ctcttttgct accattttag
    12661 ctgtgtctaa agcttttcca ccgccaaaac ctaagatagc atcagcagct tgaacttgtg
    12721 ggttggctac taacttgtca atgttagatt gtgttgaatc cgttccatag acaaagcttc
    12781 ctgtgacttt aatatttgct tcctctaaaa tagagcgaac ctcggcttct gagctgtcta
    12841 gggcacgttt gccacccacg aaagcaatgc tttgaatacg gtaagttgcc aaaacgctag
    12901 gaatttcttt gaagcaatct tctccgtagc tataatttgc cattacattt gttgtcattc
    12961 agtgtcctcc tctattccaa aggtcgatta gagacagcta ttttctaaaa gtgaatagtt
    13021 ttttgtctct tgaccaagtg attatcatat tatagcagaa tattcagaaa attggaacat
    13081 gaaaaataat ttaattttca agaataagaa tagaaaaggt aaagcgagat atacgagtaa
    13141 atactgtcag tataataatt ataagctctt gagagggtct gtattggatg caacagggag
    13201 ttcttttagg tattgatcaa gatcgcgttg gtgttttagc acctttattt tttgagggta
    13261 gtcctcaaca acttgtttat agtttgattt ttgtttgtca gagcgaccgt ctttaagaat
    13321 ccaactgata aattcaaaat ctaatttttc cgggcatcca ttggccatat caggtctagt
    13381 cttaccacga aacttacagt aacgtttgaa tgctctccac aagcagttga agcgattaaa
    13441 gtcaaaaaaa ataatctcat ctgcatctgc tagacgttct tgatatagca gttttttgta
    13501 gttaccttct ataatccatg ttcttttttc taacatgctg ctgacatcgt caatcatgtc
    13561 atcatatttt ctctcttccc aatttggggc gaagtgaata ctatcaaggt gtaatacatt
    13621 gcaattatag taatttccca attttctcgc taaagtggac ttacctgacc cactatatcc
    13681 gattattgct attttcatat actacctccg ttaaattgtt tacttgaagt taatgctgta
    13741 cttaggattt acttatttta taagggttat tcactttttt agattaatat atttttgata
    13801 taagttatcc atacacatgg taacataatt accagtaaat gtaatctaga tatcaatact
    13861 atggttgacg atattgttaa cttttgggtt caaaaaattg gggtaaaaat catcggaggg
    13921 aactgccaaa ca
//
LOCUS       DATROY010000044        13748 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.045, whole genome shotgun sequence.
ACCESSION   DATROY010000044 DATROY010000000
VERSION     DATROY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            90..2162
                     /locus_tag="VBD11_001026"
     CDS             90..2162
                     /locus_tag="VBD11_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6953988.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(2199..2609)
                     /locus_tag="VBD11_001027"
     CDS             complement(2199..2609)
                     /locus_tag="VBD11_001027"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6953989.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(2679..4028)
                     /gene="gdhA"
                     /locus_tag="VBD11_001028"
     CDS             complement(2679..4028)
                     /gene="gdhA"
                     /locus_tag="VBD11_001028"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6953990.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVGRARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            4196..4705
                     /locus_tag="VBD11_001029"
     CDS             4196..4705
                     /locus_tag="VBD11_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6953991.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(4746..6515)
                     /locus_tag="VBD11_001030"
     CDS             complement(4746..6515)
                     /locus_tag="VBD11_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953992.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(6520..8259)
                     /locus_tag="VBD11_001031"
     CDS             complement(6520..8259)
                     /locus_tag="VBD11_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6953993.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(8326..8799)
                     /locus_tag="VBD11_001032"
                     /pseudo
     CDS             complement(8326..8799)
                     /locus_tag="VBD11_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(8796..10664)
                     /locus_tag="VBD11_001033"
     CDS             complement(8796..10664)
                     /locus_tag="VBD11_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6953994.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(10675..11883)
                     /locus_tag="VBD11_001034"
     CDS             complement(10675..11883)
                     /locus_tag="VBD11_001034"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6953995.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(12068..12916)
                     /locus_tag="VBD11_001035"
     CDS             complement(12068..12916)
                     /locus_tag="VBD11_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6953996.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(12916..13296)
                     /locus_tag="VBD11_001036"
     CDS             complement(12916..13296)
                     /locus_tag="VBD11_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6953997.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(13368..>13748)
                     /locus_tag="VBD11_001037"
     CDS             complement(13368..>13748)
                     /locus_tag="VBD11_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6953998.1"
                     /translation="ILLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRM
                     ESPLDSLDHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLA
                     LTEKGLAALSNNQEVIDRHPKKKF"
BASE COUNT         4439 a         2762 c         2117 g         4430 t
ORIGIN      
        1 atatttttaa ttataaattt atattgattt ttaatgtaaa tgatagtaaa atacaaatgt
       61 aatacttatt taatttacag gagatttaaa tgaaaaagaa aattattttg aaaagtagtg
      121 tccttggttt agtcgctggg acttctatta tgttctcaag cgtattcgct gaccaagtcg
      181 gcgtccaagt tataggcgtc aatgactttc atggtgcact tgacaatact ggaacagcaa
      241 atatgcctga cggaaaagtt actaatgctg gcactgctgc tcaattagat gcttatatgg
      301 atgatgctca aaaagatttc aaacaaacta accctaatgg tgaaagcatt agagttcaag
      361 ctggtgatat ggttggagca agtccagcta actcagggct tcttcaagat gaaccaaccg
      421 ttaaaacatt taatgcaatg aatgttgagt atggcacatt aggtaaccat gaatttgatg
      481 aaggtttggc agaatacaat cgtatcgtta ctggaaaggc ccctgctcca gattctaata
      541 taaataatat tacgaaatca tacccacacg aagctgcaaa acaagaaatt gtagtggcaa
      601 acgttattga taaagttaac aaacaaatcc cttacaattg gaaaccttac actattaaaa
      661 atattcctgt aaataacaaa agtgtgaacg ttggctttat cggaatcgtt accaaagaca
      721 tcccaaacct tgtcttacgt aaaaattatg aacaatatga atttttagat gaagctgaaa
      781 caatcgttaa atacgccaaa gaattacaag ctaaaaatgt caaggctatt gtagtccttg
      841 ctcatgtacc tgcaacaagc aaggatgata ttgctgaagg tgaagcagca gaaatgatga
      901 aaaaagtcaa tcaactcttc cctgaaaata gcgtagatat tgtctttgct ggacacaatc
      961 atcaatatac aaatggtctt gttggtaaaa ctcgtattgt acaagcgctc tctcaaggaa
     1021 aagcctatgc tgatgtacgt ggtgtcctag atactgatac acaagatttc attgaaaccc
     1081 cttcagctaa agtaattgca gttgctcctg gtaaaaaaac aggtagtgcc gatattcaag
     1141 ccattgttga ccaagctaat actatcgtta aacaagtaac agaagctaaa attggtactg
     1201 ccgaggtaag tggcatgatt acgcgttctg ttgatcaaga taatgttagt ccggtaggca
     1261 gcctcatcac agaggctcaa ctagcaattg ctcgaaaaag ctggccagat atcgattttg
     1321 ccatgacaaa taatggtggc attcgtgctg acttactcat caaaccagat ggaacaatca
     1381 cctggggagc tgcacaagca gttcaacctt ttggtaatat cttacaagtc gtcgaaatta
     1441 ctggtagaga tctttataaa gcactcaacg aacaatacga ccaaaaacaa aatttcttcc
     1501 ttcaaatagc tggtctgcga tacacttaca cagataataa agagggcggg gaagaaacac
     1561 catttaaagt tgtaaaagct tataaatcaa atggtgagga aatcaatcct gatgcaaaat
     1621 acaaattagt tatcaatgac tttttattcg gtggtggtga tggctttgca agcttcagaa
     1681 atgccaaact tctaggagcc attaatcccg atacagaggt atttatggcc tatatcactg
     1741 atttagaaaa agctggtaaa aaagtgagca ttccaaataa taaacctaaa atctatgtca
     1801 ctatgaagat ggttaatgaa actattacac aaaatgatgg tacatatagc attattaaga
     1861 aactttattt agatcgacaa ggaaatattg tagcacaaga gattgtatca gacactttaa
     1921 accaaacaaa atcaaaatct acaaaaatca accctgtaac tacaattcac aaaaaacaat
     1981 tacaccaatt tacagctatt aaccctatga gaaattatgg caaaccatca aactccacta
     2041 ctgtaaaatc aaaacaatta ccaaaaacaa actctgaata tggacaatca ttccttatgt
     2101 ctgtctttgg tgttggactt ataggaattg ctttaaatac aaagaaaaaa catatgaaat
     2161 aataaaaata ggtttggata aaatatccaa acctattttt atatcaatat gccttccaaa
     2221 tgatctaact cgtgttgaca aatttgagct ggaaaatctg tcaacttgat ttgctgttct
     2281 ttccaattga tatcacgata agaaatcgta atgtgatgat atcgttgtgt acttctgcaa
     2341 cctacgagag aaaggcaaga ttcttctgtc tcatatggat ccgattttgc aactaataca
     2401 gggttaaaca ttactaaatt tgtaataccc acattgatga taatgacacg cttcagacta
     2461 ccaatcatat tggctgccat acctacacaa taattttgat tagcatgcaa agtatcctgt
     2521 aaatcttttg ctaagctcac atctgctcta ctagccattt gtgatttttg ttgtaaaaag
     2581 aaagtatctc taacaattgg ttttatcatt tattttaacc taactctatt atgataatac
     2641 tagtatacca taggttagct aaggactatg ataagagatt aaacaatacc ttgtgcagac
     2701 atagcttgag caacttttaa aaagcctgca atgttagcac ctagaaccag gttaccacta
     2761 tcgccaaatt catcagccgc tttggaagca ttcacaaaaa tagttttcat aatacgttgc
     2821 aattcttgat caacttcttc aaaagtccat gccctacgac tagaattttg tgccatttca
     2881 agcgctgaaa cagctactcc tcctgcattg gcagctttag caggaccaaa agcaacccct
     2941 gccttttgga aaactttaat agcacccaag gtagatggca tatttgctcc ttctgttact
     3001 gccaagacac cattttcaac taataactta gcgtcttctt cgtctaattc gttttgagta
     3061 gcacacggga aagctaaatc tgctttaata gaccagatac taccttgatc tgctggtgtg
     3121 aaattagctt ttggatgttt ctccgtatat ttaacaatac gtgctcttcc aacctctttt
     3181 atctgtttca atgtttcaag gtcaataccg tctggatcat aaacataacc tgaagaatct
     3241 gacacagcta caacagttgc acccaactct tgtaattttt cagtagcata aatagccacg
     3301 ttaccagatc ccgaaaccag tgcaactttg ccagttaaat cttgaccacg cgctgccagc
     3361 atttctttag caaaatagac tgccccataa ccagttgctt ctgtacgagc caaagagcca
     3421 ccataagtca gtccttttcc ggttaaaacc ccattttgat aaccatttaa acgcttatat
     3481 tgaccataaa gataaccaat ttcacgtcca cctactccaa tatcgccagc tggcacatct
     3541 aaatctggtc caatgtattt ttgaagttcg gtcataaaac tttgtgtgaa acgcatcact
     3601 tcattatctg atttcccttt tggatcaaag ttcgaccctc ctttgccacc accaattggt
     3661 agacctgtca gtgaattctt aaaaatttgt tcaaatccta aaaatttgac aatagattgt
     3721 gttactgtag ggtgaaaacg taaaccacct ttataaggac caatagctga tgaaaattgt
     3781 acacgatatc ctcggttaac ttgaacttgg cctttatcat ctacccatgg aactctaaaa
     3841 gaaatcacgc gctcaggctc aaccaaacgt tctaaaagat tttcttcaat gtactgaggg
     3901 tatttatcaa aaactggtac tagtgactca aaaacctctt caaccgcttg tagaaattct
     3961 tcctcatgct cattttgctt cttcactttc tctaaaacac ttgctacata tgtctttccg
     4021 cttgtcattt attttcctcc gtattgtttt aatatagtta gcattataaa ttgtcagaat
     4081 tttcttgtca actatttgct atggtatcat tattaggagc aattttaaag gtaattattt
     4141 gacttttact gactttcgtt ctataatgag acaaatattt gattggagta tctatatgaa
     4201 atggttccca cttgttggag gtataattgc cttattagct ggcttctatc tctttataaa
     4261 tcctgtaacg gttgttgcaa ctctgggttg gttcattgct ttatttatct ttatttatgg
     4321 catttcaaat ttagtacgct acttgcaagc tgattcttat caccgtagcc cttggtatct
     4381 ggttcagagt atccttgcta ttatttttgg tatcatttta ctatccagct cagcaatgtc
     4441 actttcaaat actgtcatga ctattttagc atactggcta ctggtatctg gtattttacg
     4501 acttttgggt ggtttccaaa tgcaaaaaat taatttccca ggtgccaaat cctataaacg
     4561 ttcggcttat attgcactca ttttaggtat tattttactg gttcaaccta ccttatcagc
     4621 agccatcatt ggaagatttg tagcctttat attaatagca attggagctt cggctattta
     4681 cacttttact cgtattaatt attaaaagga catgtttcgt gtcctttttt tatctattga
     4741 tgacgtcatt tactatttga aaattggcta ttatataatt cgtaataaaa tcctttatct
     4801 gctaggagtt tctggtgatt accttgttcg atgatttgac catcctttag aactaaaatg
     4861 ttgtctgcct cttgaatcgt tgatagacgg tgtgcaatga caaaactagt tcttccttcc
     4921 atcaattttt tcatagcttt ttgaataagc aattccaacc ttgtatcaac agatgaagtc
     4981 gcttcatcta aaattaaaat tttaggatct gctaataaag cacgtgcaat agtcaacagt
     5041 tgcttttgtc ctaaggagat attgcttgat tcttgattca tcaccatatt gtagccacca
     5101 ggtaacgttc gaataaagtg gtcaacattc gcagctttgg cagcagcaac tatatcctca
     5161 tcactggctt caagattacc gaatctaaga ttttctttta ttgtaccttc atataaccaa
     5221 gcatcttgca acaccatgcc aaattgttgc cggaaatctt ggcgtgatag gtgtctaata
     5281 tcgtgaccat ctacggtgat agcaccttca ctaacatcgt agaatctcat gagtaagttt
     5341 ataagtgttg tcttacctgc acctgttggc cctacaattg caatcatttg ccctggttct
     5401 acatctaagt taaagtcgcg gataagtggc ttatccggag aatagtgaaa atcgacacca
     5461 tgaaagctga cttgaccggt taagtcatgg ctcaattttt cagtgacttg attggcttcc
     5521 tcttcttcat ctaagacttc aaaaatacgt tctaaggaag attttgcact ttgcaaaaca
     5581 cctgctaatt gtgtaatggt ttgaacgggt tgactaattt gccaaacata ctgcacaaat
     5641 gcctgcatgt taccaattgt taaggtacca gcaataactt gtaaaccgcc aacaaatgca
     5701 atgataagat aaataaaatc agagatacta ttcaatactg gcatcataat tcctgaaatg
     5761 aagctagctt tgaaacctac atgacgtaga ttatccgtta tatcacgaaa ttcttgacta
     5821 gacgcctctt ctctaccata taattttatg atattgaaac cagttaattt ctcttgtaca
     5881 aaaccattca gttctcccag aatctttgcc tgttctttaa agtaaggttg ggatcttttt
     5941 agtatcgctt gtgcactaaa ataagttaca ggaatacaag caatgataat catggctaat
     6001 ggtacattta gataaagtac cataacgact actagaataa tacttaaaaa agcattgata
     6061 atctgtaaaa agctctgttg taaagcattt gagaccgttt caacatcact tgtaaaacgt
     6121 cccaacatat ctccaaattg gtgcttatca aaatacgata caggaacttt attgatttta
     6181 cgattcaaat catgacggat atcacggata cttttttgaa cgacagtagt cataaaataa
     6241 tttgagccat aagaacctaa ttcatagaaa acacctctga aaaaataaat aatcagaata
     6301 ccagcaatat aaggaacgtt caattctgcc ccagaaactc ccttagccat atcaacaagg
     6361 ttagcagtca actctgttat cgctaaccct aaaataaaag gctccagaat actcataaaa
     6421 ctagataaaa ctttcaaaaa aatcgctaag aaaagagtag ctttgtagcg agttaggtaa
     6481 gaccataatc tcaaaaaaat agattttttt ctcatacaat cactctcctt ctgataagtt
     6541 ttggttcttc agttgtgact cagcgatttc acgataaata gcattatttt ctattaattg
     6601 agcgtgggta ccacgaccga caatttcgcc ttcatcaagg acaataatct gatcagcatc
     6661 cataatcgta cccacccttt gagcaactat caaaaccgta gaatcaccgg ttacttcttt
     6721 tagacgcgct cttaaagtag cgtctgtctt ataatcgaga gcagaaaatg aatcatcaaa
     6781 aatatataaa tctggatctt taacaacagc cctagcaatg gataaccgtt gtttttgacc
     6841 cccagaaaga ttgctcccac cttcagctaa atgcgtttca aaggcttctt ggtgactctc
     6901 aataaactct ttagcttgag aaatatcaac cgcttgtcta agatcatcaa tagtagcatc
     6961 agcttttcca tattttaaat tctctcctat tgtccctgta aataaaagag ctttttgggg
     7021 gataaatcca atcttttggc gaagtgattt aaggttataa tctcttacat caactccatc
     7081 tactaagatt tttccaagtg tcacatcata aaaacgtgga atcaaattaa caagagaaga
     7141 ttttcctgaa cctgttgaac caataaaagc aattgtttct ccaggcttag ctttaaaaga
     7201 aatatcatgc aaaacgggac tctctgtttc tcctggataa gcgaatgtta cattatcaaa
     7261 ttctaaatgc cctttaagtt tcgtatccgt aacaccttcg gtattaggat taatagaaat
     7321 cggcatatcc attacctctc taatacgatg gctagatacc accatacgag gatacatagt
     7381 aaaaagattg gcaaatagca aaaatgaaaa gagagcatgg aagctatatt cgataaaagc
     7441 aactaaatcc cctattttaa tagcacctct ttgtaaggga tccaaagcaa accaaacgat
     7501 agccacaatc attgcaataa taatttgaac gaaaagtggt tctgttagcc ctgttaattt
     7561 aaaaagacca gttgaagtat ctgtgtaacg ttggttagcg acttgaaatt tttgtgattg
     7621 aaaattctct cttgcaaagg ccctaacaac gcgtaaccct gttaaatttt cacgaacata
     7681 ttgattgatt ttatcaagca tagtctgttg tctttcagat aaaggttttg tttttatagc
     7741 tacatataaa atgactccta ccaaaagagg catcgcaacc gctacaagcc aagccaaaga
     7801 tggactcgta attagtatca taaccacgct aaaaatcatt accataggag ttactaggcc
     7861 taaacgtaaa gacatttcag caaattgcat caaaacaaaa gtatcgcttg tcatacgtgt
     7921 cactagtgaa gatacaccta tctgttcata ttcatgatgg gagtattctt gaagcttagc
     7981 atacatatca ttacgcatat ctctaatcat tgttgtcgtt aagcgactag atgcgtaagt
     8041 catcgtaata cgcccaataa tacctagtac aacaaatata aacatgatga acgtccacag
     8101 ataaactcca gtccgatcac cttttgtaac gccattatca atcatacccg ctaaggctgt
     8161 gggcattcct aggataaccg tcacaaaaag catagctcct agtaaatcaa gccataacca
     8221 atttgggtac cttttcaaat aagaccaaat atatgccata tatgtctcct tttctttcct
     8281 cttatctcaa aataattaat ccatgttcac taatccactt tcaacttata aatctttaat
     8341 catccaaata tccatagctg tatgaccttg atcatttgct attggttgcg ataaggcaca
     8401 atatcccata tgcttataaa ccgcagttgc cctactcaaa atactggctg tctctaaata
     8461 aagttgtcta tacccaattt ttcgagcttc tacttcaatc attttcacta aatcagtagc
     8521 aagcccctta ccacggaaac gttctgcaat gtacaccttc tgcatctctg caattagatt
     8581 tttcagcgga ccaaagccgc ttcagccaat aatctcatct ctctcctcaa tgacaaagaa
     8641 tcctgacttc tctatttttt cgtagtatga ggtcaagtga tctaagtgag ggtctgaata
     8701 tgctgtacca ggtttatcta aatcataggc gcgtaaactc tgtcgaatta attgtgtaac
     8761 tgcttggtca tcatttttaa taatcggacg gataatcatg tgtctaaccc actaaggtac
     8821 tcataacggt catacttttc tagaagtgct tcattttttg catctaattc cttttgtaaa
     8881 tcagataacc ttgtaaaatc actaccacat gtttgcatat catcttctat acgagtgata
     8941 gtattttcca atatcatcat atcgtcttca attgtcgccc attcttgttt ttcaaagtaa
     9001 gacattcttt tacgactttg cttttcacga cttgtcttgg tactctcttt tttactgata
     9061 acttcattat tttgctcatt aaatgctttt tcatctaaat aatcagtata attaccaaaa
     9121 aattcacgga tatcgttatc ttcaaatgca ataattttat tagccacttt atctaaaaag
     9181 taacgatcgt gactaactgt aatcacagga ccaccaaagc cttgtaaaaa attttcaaga
     9241 acagttaatg tagcaatatc taagtcattt gtcggctcat caagtagtaa cacgttaggc
     9301 ttttcaatca ggatttttaa aaggtaaagt cgttttttct caccacctga taattttgca
     9361 atttgtgttc catgtgtcga acgtggaaag agaaattgtt ccaatagttc tgtcacactt
     9421 gttgtaccga cactagtttt aacctcatct gcaacctctt gcaaataatt aataacacgt
     9481 tttgagccat ccatattatg aagttgttgt gaaaagtaac ctacacgtat cgtttcacca
     9541 atagagacat tacccgaatc cggctgtaaa tcttgaacaa ttaaattaag taaggttgac
     9601 tttccaacac cattatctcc aacgatgcca atacggtctt tattttgaat taacaaatta
     9661 aagtctttca agatagattt atctgggtaa gaaaaagaga cattttcaaa attaataacc
     9721 tttttcccaa ttcgacttgt ttcaaatgtc atttccaaat cgcttgtatc gcttgtttgg
     9781 tgtaaatcgt tttttagatt ttgaaaacga ttaatacgag cctgttgttt cgttgcacga
     9841 gcttgtggct gagtacgcat ccaagctagt tcctgtttat aaagctgttt ctttttatgt
     9901 aaactagcag catcacgctc gtcttgttct gcacgaagtc ggacataatc ctgataattg
     9961 ccttgatatt ctgtaatctg tgccttatct aattcaaaaa tacgtgttgc aacattgtct
    10021 agaaaataac gatcatgagt tataaaaagc actgtctttt tactattttt caaaaaattc
    10081 gttaaccatg caatagtgtc aatatctaag tggttagtag gttcgtctaa gagcaataaa
    10141 tctgcatcat ttaataatac ttgcgctaat tgaacacgtc ttcgtaatcc tcctgataat
    10201 tcaccaaccg acaactgcaa atcagtaata cctaatttgg ataatactgt tttgacttcg
    10261 ctctcaatag accaagcatc taaagaatcc atttctgcca ttactttctc tagacgtgat
    10321 tgcttacttt cttcgtagtg attaagcaat aattcatatt ctttaattaa agccatctct
    10381 cttaagtcag aagaaagtac ggtgtccaaa attgtctgag aatcatcaaa gtctggttct
    10441 tgttttaaat aagcaatctt ataatcatta gctgatgaaa aaggggaacg atcaccatca
    10501 aaacctaatt cacccgaaat aacatctaat agtgttgtct ttccagttcc attgacacca
    10561 ataatcccaa tacggtctaa actatggatg ataaatgaaa cattactaaa gaccgtctta
    10621 tcaccaaccg acttagtcaa tccatctact aaaaaatcgc tcatataatc ctctctaatc
    10681 catcaagtac tgcttaacaa aatgtaaaat agcctcttct tcattaatca gttgtcctaa
    10741 aacaatggct aattcaattt gagagataat atctcccatc tgtggaccag gttttattcc
    10801 taatttcttg attaaggtgc caccattgac aactatctca tgcctatcat ggatagctaa
    10861 ctcactatct agttgttcta ttttttcgaa atcaaccatc aagcctcttg ctttgcgaat
    10921 accttccgct tgttttatta tagtttttcc ataacgataa agatgttctt tagtcaatcc
    10981 catttgttca cgaatacgat aaacatccac tatatgctca acatcttttt gaaagtgagt
    11041 tgatgtcttc cattttttta aaaaagtctt tgtgtgttca acttccatac tcaagatgag
    11101 tgatgcccaa gcttgttctg atgtctcaaa taaaaaatct ttatctaact gggacaaaaa
    11161 ggcgcttaat tgtaattccc tattttttaa gcatggtaga taattaaaag catgactatc
    11221 tattagtgcc agcattcctt tacgccaata aggtgctaat agaagtttat caaattcaat
    11281 aaaagaacgc tcaacagata ttttctccaa taatggcgcg tgctttttca ttgcctcaaa
    11341 agttgttgtt tcgatatcaa aatttagact agcagaaaaa cgcaaacctc gcataattcg
    11401 aagagcatct tcattaaaac gctcactggc gagtcctact gctctaagaa ggtgattatc
    11461 taaatcatcc aatccatgaa agagatcaat aacttctcca tcttcattta aagcaaaggc
    11521 attaactgtg aaatctcgac gttttagatc ttcttctagc gacctaacaa aattgacctg
    11581 actcggacgc ctataatcaa cataaacctc ctcagtccta aaagtggtaa tctcatattc
    11641 accacctttt tccaatacta agactgttcc atgttcaata ccaacatcaa ctgtcctttt
    11701 aaaaatctgc tttgtttctt ctggataaga tgacgttgct atatcaacat catgaattgg
    11761 acggtcaagt aaaacatccc gaacactacc acctacaaag tatgcttcat aaccggcttt
    11821 ttttatcttt tttaatattg gtaaagcctt ctgaaactca gaaggcaaat aatttaatct
    11881 catcatacta ttccaaatct aaacgaacta aagtcgcatt attatttctt ataaatggat
    11941 tcagaaacaa aaagaactgc ctaagggaca ctccctacct actcaaatta tcaaaaaaga
    12001 atctcctaac ttcttctttg atatctgcaa taaggatcaa cctccaagca actcttttta
    12061 tcacacatca attttttggg gtaataccaa aggcaaccgc accttcacca agatgagttg
    12121 caataacgcc accaaatgat acaatttcta aatcgtcttt aagaccagct ttagctaaca
    12181 aattatataa ttgttctgcc ttatcttgtg ctctagagtg aataatagca atatcatatt
    12241 caccatctgc tgtcatttct ttgacaatct cagccaagcg ttttaaggct tttttctctg
    12301 tacgcacttt ctcatagaca acgattttac cttcttcatt aaaatgtaag accggcttga
    12361 tacttaaaag gttaccaatg atagctgaac cgtttgacaa tctaccacct tttactagat
    12421 gattcaaatc attaacaaca ataaatcctt caattttttc aatttgtgat tgtatcttgt
    12481 taactatgac atcaaaatcc atcccttcac gtgaacacat caaagcattt ctaactaaat
    12541 tgccctgtgg tgctgaagtt attttagtat ccggaaaagc aattgtcaaa tttggatgtt
    12601 cttcaataag aaactgaata ttctgccaga aacctgatat ccccgctgca atgaataaac
    12661 caaggacgtg tgtataccct tctttctcca attgacataa gagatcatct agttcagcta
    12721 agctaggctg acttgtttta ggtaattctt ttgatgccgc tagcttatca taatattggt
    12781 ctaatgtcag attttgtccc tcaatataag ttttaccatc aatgataatt ggaatatcta
    12841 aaacatagac atcttcatga ttttcgatag cctcaggcaa gtaagctgag gtatccgtaa
    12901 taagtgctaa tttcattaac tattaaactc caatgttact cctggtctat ctaaagcaat
    12961 ttctgtaact tcataagtca gacgtttaac gatttctaaa agaggagctg ctagattctc
    13021 aacttcatct tgactaaact cactaggctc attgataagg cgcccttgga tatgagctat
    13081 ttgtgaaatg acaccagaaa taacaaactc atcacgtaca ataacaaatt gtaacactgc
    13141 tacaatgctc gttacttttg ctgcttcatc tctattagct aattggaaag taacttcaaa
    13201 atttgttttt ggggtaccat tttcctcttc ccattctaaa ttgcgcgcat catagtggta
    13261 ttgattgaca aattcttgtt cacgaatgac ttccatgtat tttctccttg atagacaata
    13321 tgtattatta tagcataaat ttgtagattc ttaaagcagg tgggaatcta gaacttcttc
    13381 ttaggatgtc tatcaatcac ttcttgattg ttactcaagg ctgctaaacc tttttctgta
    13441 agtgccaacc ctctgacaac aaacaacttc cctaattcct cttctgtaaa agcaatagct
    13501 attgcctgat ctaaatcaga gcgtttcatc tttgacaagc cttttacgtt tttttctttt
    13561 aagaatagtt ttaaaagtgg agcagataag tgatctaaag aatcaagcgg tgactccatt
    13621 cttacaaatc cattttttat taaaaattct aggtgttctt ttccatttat accataaatg
    13681 tattcaaaat attttgaaaa acttgtttca gttgtgaaag ttccaaactg tatacgccat
    13741 aataagat
//
LOCUS       DATROY010000045        13422 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.046, whole genome shotgun sequence.
ACCESSION   DATROY010000045 DATROY010000000
VERSION     DATROY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            120..1391
                     /gene="murA"
                     /locus_tag="VBD11_001038"
     CDS             120..1391
                     /gene="murA"
                     /locus_tag="VBD11_001038"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6953999.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            1393..1584
                     /locus_tag="VBD11_001039"
     CDS             1393..1584
                     /locus_tag="VBD11_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6954000.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            1659..2516
                     /locus_tag="VBD11_001040"
     CDS             1659..2516
                     /locus_tag="VBD11_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6954001.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            2807..3847
                     /gene="pheS"
                     /locus_tag="VBD11_001041"
     CDS             2807..3847
                     /gene="pheS"
                     /locus_tag="VBD11_001041"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6954002.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            3930..4450
                     /locus_tag="VBD11_001042"
                     /pseudo
     CDS             3930..4450
                     /locus_tag="VBD11_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            4504..6909
                     /locus_tag="VBD11_001043"
     CDS             4504..6909
                     /locus_tag="VBD11_001043"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6954003.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(6978..7880)
                     /locus_tag="VBD11_001044"
     CDS             complement(6978..7880)
                     /locus_tag="VBD11_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6954004.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            8058..11291
                     /gene="rexB"
                     /locus_tag="VBD11_001045"
     CDS             8058..11291
                     /gene="rexB"
                     /locus_tag="VBD11_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6954005.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            11281..>13422
                     /locus_tag="VBD11_001046"
     CDS             11281..>13422
                     /locus_tag="VBD11_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEO6954006.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKI"
BASE COUNT         4448 a         2120 c         2668 g         4186 t
ORIGIN      
        1 cattttttat tggaagtgat tcagttgtgt caatcaatct tttgggctct ccaataaagg
       61 aaatattagc tgaactatga tataatcaac ttactattgt tataagaatg gagattcaaa
      121 tggataaaat tattgtggaa ggtgggcaaa cgcggcttca aggtcaagtt gttattgaag
      181 gagctaaaaa tgcagtttta cctcttttag cagccacaat tttaccaagc caagggaaaa
      241 cattattgac caatgttcct atcttgtctg atgtatttac aatgaacaat gttgttcgtg
      301 gtttagatat tcaagttgac tttaattgcg ataaaaaaga aatcttagtg gatgcctcag
      361 gtgatatttt agatgttgca ccttatgaat ttgttagtca aatgagagct tctattgttg
      421 ttttgggacc tattttagct agaaatggcc atgctaaggt ttcaatgcca ggcggttgta
      481 caattggtag ccgaccaatt gaccttcatc ttaaaggttt agaagctatg ggagctacga
      541 ttacacaaaa tggtggtgat attactgccc aagctgaaaa gcttaagggg gctaatatat
      601 acatggattt tccctcagtc ggtgccactc aaaacttgat gatggctgct acacttgctt
      661 caggcacaac aacgatagag aatgctgcac gtgaaccaga aattgtagac cttgcgcagt
      721 tactaaataa aatgggagct aaagtcaagg gtgctggtac ggaaacatta acgatcattg
      781 gtgttgatgc tttacatggt acagaacatg atgttgtcca agatcgtatt gaagctggaa
      841 cctttatggt ggcagctgct atgacttctg gaaatgttct agtaaaggat gctatttggg
      901 aacataaccg tcctttaatc tctaaattga tggagatggg tgttgaagtg tcagaggaag
      961 aagatggtat tcgtgtaaaa gctgatacta aaaaattgaa gccagtgact gtgaaaacac
     1021 tccctcatcc aggatttcct actgatatgc aagctcaatt tacagctcta atggcagtag
     1081 ttaatggcga atcaactatg attgagacag tttttgaaaa tcgttttcaa catttggaag
     1141 aaatgcgtcg catgggatta caaacggaaa ttttacgaga tactgcaatg attcatggtg
     1201 ggagagcctt acaaggtgca ccggtcatgt ctactgactt acgagctagt gcagctttaa
     1261 tcttggcagg aatggttgcg caagggcaaa cagtagttgg ccaattaacc catttagacc
     1321 gtggttatta tcaattccat gaaaaattag cggctcttgg tgctaatatt aaacgtgtaa
     1381 gtgaggcgta atatggcatc tagtggttgg aaatatgtac taaaacaaat cggtctaata
     1441 gtattagtca tactgttagc cttgctgttt ttagcggttg gattgatgtt gggttatagt
     1501 gtttttggtg acggagagca tgcttactct attttatcgc tggataaatg gcaaaatatt
     1561 attggaaaat ttttaggaaa atagaaagag ccactataag ttatttgatg cttatagtgg
     1621 tttcctttga gagttaattt tagcagtaga ataatacaat gacaaaaaaa caaaaacaaa
     1681 taacagcttc gattgtaact ttgctttttt cattactggc aatagggtta ggtgaaagta
     1741 aatctttgcc taatgatcac attttaaaac aggtgacaga tttaattatt agtaatgaat
     1801 ctggcaaaaa tacttccctt aaaaatatat caggaacccc tagtagagag ttatctgaat
     1861 ctgttttgac tagcaatgtt aaaaaacaat tgggaacaaa tatagcttgg aaccagtcag
     1921 gtgcttttat cattaatcaa aataaaacag atttgaatgc taaagtgtca agtgcaccat
     1981 atgctattaa tgaaataaag aaggttaata accaaattgt tccaactaaa gcaaatgcct
     2041 tattaacaaa agcaactcgt caatatcgaa atcgagaaga aacagggaat ggtagaactt
     2101 attggaaacc agctggatgg catcagataa atggattaaa agggagctat aaccacgctg
     2161 tggacagagg ccatttaatt ggttatgctt tagtagggag tttaagagga tttgatgcct
     2221 ccacaagtaa tcctaaaaat attgccacac aagcagcttg ggcaaaccaa gctaatagca
     2281 accaatcaac agggcaaaat tattatgaaa ctttagttcg taaagcattg gatcgtcata
     2341 aaactgttag gtatcgtgtg actttaattt atgacagaga taatctttta tcttcgggat
     2401 cacatattga ggctaaatct tcagatggaa gtttagaatt taatgttttt attcctaatg
     2461 tacaatcggg attattattt gattatgcaa caggaaaagt gaagcagaca aagtaatctt
     2521 tatctaatag tgcttgttaa agactgaaaa atttgataaa atagtcctta attgaataga
     2581 ggtctgtgag actagtaaac caagggaagc tgcttaggga gttcagccgt gactgcaagc
     2641 tgaatcagtg aaagagggtg gaattcactc acaaatagga ctataattaa ggtctgattg
     2701 gtcgatcaga tagaaaacgg atggtaccgc gtgtcaacgc tccgattaag ggggagtggc
     2761 acgctttttt tgttttcaaa gctacttatt ttgacttgga ggaaagatgg atttacaaaa
     2821 acaattagaa gagttgaaaa tatcaactca agaaaagctt aaagaaatga ctgggaacca
     2881 cactaaagaa ttacaagact tacgtgtaca agttttagga aaaaaaggtt ctctaacaga
     2941 attactaaaa ggtctgaaag atttatctaa tgatttacga ccagttgttg gaaaacaagt
     3001 caatgaagta cgtgatattt taacaaaagc gtttgaggaa caagctaaag tagtggaagc
     3061 tgcgaagatt caagcacagt tagagtctga gagtgtcgac gttaccttgc ctggtcgtca
     3121 aatgacatta ggtcaccgtc atgtcttaac acaaactagc gaagagattg aagatatctt
     3181 tttaggtatg ggattccaag tggttgatgg ttttgaagtt gagaaagact actataattt
     3241 tgaacgaatg aatttaccaa aagatcatcc agcccgtgat atgcaagata ctttttatat
     3301 tacagaagaa atcttgctac gtactcatac ttcgcctgtt caagcacgaa caatggacca
     3361 acatgatttt tctaaagggc ctttaaaaat gatttctcct ggacgtgttt tccgtcgtga
     3421 tactgatgat gctactcata gccatcaatt ccatcaaatt gaaggcttag ttgttggaga
     3481 aaatatttca atgggcgatc tcaaaggaac acttcaactg atttctcaaa aaatgtttgg
     3541 agcagagcgt aaaatccgac ttcgtccttc atatttccca ttcacagaac cttcagttga
     3601 agttgatgtc tcttgcttca agtgcggagg taagggatgt aatgtctgca aacagacagg
     3661 ttggattgag atattgggtg ctggtatggt tcacccaagt gttttggaaa tgtctggtat
     3721 tgattctgag aaatactccg gttttgcctt tggcttaggt caagaacgta ttgcaatgtt
     3781 acgctacggt atcaatgaca ttcgtggctt ctatcaaggt gatgttcgct tcacagatca
     3841 attttaagat tttagatagc aagtagcatc attggtgtga tgataaattt atgttaacgc
     3901 tattacctta ctcaatagga ggttttctaa tgtctcaaat tacttttaaa aaggttggcc
     3961 ttgataatgt gaatatttta cagaatcttg ctatagaaac ctttcggcag acgttttctc
     4021 atgataatag tgaggaacaa cttcaagcat ttttcaatga gtcttacact ttaccagtat
     4081 taaaatcgga aattacccat gctgaatctg atacttattt tgtttatctt gatacagatt
     4141 tagtgggtta tttaaaagta aactggggaa gtcagcaaac tgaaaaaaac ttgataaagc
     4201 atttgaaatt caaaggattt atcttctaga tgcctaccaa gggaaaggta taggtaaggc
     4261 tactttcgaa tttgctttgg atttagctta taaatcaggc ttagattggg cttggttagg
     4321 tgtttgggag tttaatcata aagctcaggc attttacgct aaatacggat ttgaaaaatt
     4381 ttctgagcac caattttcag taggtgataa agtggatacg gattggttat taagaaaaag
     4441 tttacactaa aaactacaaa gcacttgtat aaaaataaaa gaaaataaat agagagaaat
     4501 tacatgttag tatcgtataa atggttaaaa gagttagtgg atgttgatgt cacaacagca
     4561 gaactagctg agaaaatgtc aaccactggt attgaggttg aaggtgtgga gacaccagcg
     4621 gaaggtttat caaaattagt tgtaggtcat attgtttcgt gtgaagatgt tccagatacc
     4681 cacttacatt tgtgtcaagt tgatacaggg gacgatgaac ttcgtcaagt tgtctgtggt
     4741 gctccaaatg tcaagactgg tattaatgtt atcgtagcgg ttcctggtgc tcgtattgca
     4801 gataactata agattaaaaa aggtaaaatc cgtggtatgg agtcacttgg aatgatttgt
     4861 tcacttcaag agttgggctt atcggaaagt atcattccaa aagagttttc tgatggtatc
     4921 caaattttac ctgagggagc tatacctggt gatagtattt tctcttatct tgacttagat
     4981 gatgaaatta ttgaattatc aattacacca aaccgtgcag atgctctttc aatgcgtggt
     5041 gttgcccatg aggtagcagc aatttatggg aaaaaagttc attttgaaga aaaaaatctc
     5101 attgaagaag cagaaagagc agctgataag atttctgttg tcattgaatc agataaagtt
     5161 ttatcttact cggctcgtat tgttaagaat gtaacagttg caccaagtcc acaatggctt
     5221 caaaataaac tgatgaatgc tggtatacgt ccaattaata atgttgttga tgtgacaaac
     5281 tatgttcttt tgacttatgg tcaaccaatg catgcttttg attttgataa atttgatggg
     5341 acaactatag tagcacgtaa tgctgagaat ggtgaaaagt taattactct tgatggtgaa
     5401 gagcgtgact taatagctga tgacttggtc attgcggtaa atgatcaacc agttgctttg
     5461 gcaggcgtta tgggaggaca atcaactgaa attgatagtt cttctaagac agtggtcctt
     5521 gaagcggcag tatttaatgg aacaagtatc cgcaaaacaa gtggaagact caaccttcgt
     5581 tcagagtcat cttctcgctt tgaaaaaggt atcaattatg atacggtttc agaggctatg
     5641 gattttgcgg ctgcaatgtt acaagaattg gcaggcagtc aagttctttc tgggcaagtt
     5701 acagaaggag ttcttccaac tgagccggta gaggtatcaa cgactcttgg ttacgttaat
     5761 actcgtttgg gaacagaact tacctatact gatattgaag aagtatttga aaaactcggc
     5821 tttgctattt ctggttcaga agtgaaattt actgtgttgg taccgcgtcg tagatgggat
     5881 attgccattc aagctgactt agttgaagag attgcccgta tttatggtta cgaaaaatta
     5941 ccaacaacat tgccagaagc cggagcgact gcaggagaat taacgagtat gcaacgctta
     6001 cgtcgtagag tccgtacggt tgctgaagga gtaggattat ctgaaattat cacctatgct
     6061 ttaacgactc cagaaaaagc agtgcagttc tcaactcaag caactaatat aactgaactt
     6121 atgtggccga tgactgttga tcgctcagca cttcgccaaa atgttgtttc aggaatgctt
     6181 gatacaattg cttataacgt tgctcgcaaa aacagtaacc ttgcagttta tgagatagga
     6241 aaagtttttg aacaaactgg taatccaaaa gaagatttac caactgaagt agagacgttt
     6301 acttttgcct taacaggtct agtagaggaa aaagatttcc aaacaaaagc caagccagtt
     6361 gatttcttct acgccaaagg tattgtagag gcgctcttta ttaaattaaa attagatgtt
     6421 acttttgtag cacaaaaagg tttagcaagt atgcacccag gtcgtacagc aactatctta
     6481 ttagatggga aagagattgg ttttgttggt caagtacacc cacaaactgc aaaacaatat
     6541 gatattcctg aaacttatgt tgctgaaatc aatctatcaa ctattgaaag tcaaatgaac
     6601 caagcgttga tttttgaaga tattacgaag tatccgtctg tatctcgcga tattgcatta
     6661 ttacttgcag aatctgttag tcatcatgat attgtgttag caattgagtc atcaggggta
     6721 aaacgtctaa cagcgattaa actgtttgat gtctacgctg gaaataatat tgcagaaggg
     6781 tataaatcaa tggcttatag cttaactttc caaaacccta atgacaatct aacggacgaa
     6841 gaagtggcta aatatatgga aaaaatcact aaatcactcg ttgagaaagt taatgcagaa
     6901 ataagataat ttatatctta cgttgaaaaa taaataaaaa acagcatcaa ttaagatgct
     6961 gtttttttat ttaaaattta tagatgttct gcggagaaag tatcaccgtg ttggatgtca
     7021 ccatattgaa agcctttgtt aaaccagcgt tggcgttgtt gagctgttcc atgggtaaag
     7081 ctatcaggca ctaattttcc gtaggtttct ttctgaaggg tatcgtctcc gacggcgtgg
     7141 gcagcattca tggcctcttc aaagtctcct tgttctaaga gattttttcc cctgatgtag
     7201 tgagcccata cccctgcata ataatctgct tgaagttcta gccgaacatt taaagcattt
     7261 gcttctttct tagtaagtcc gtgtcgcatt ctattatact tatccataat gcctaactct
     7321 gtttgaatgt ggtgaccaac ttcatgggcg atgacgtagg ccatagcaaa atcaccagta
     7381 gcaccatatt tatgtgataa ttcattgtaa aaagaaatat caagatagat ttttttatct
     7441 gctgaacaat aaaatggttc tgaagcagat tcacctatac cacaacctgt ttgaatcgaa
     7501 ttggtgtaaa gaacaagttt tggttcctta taatttccaa aaccttgggt ttggaattct
     7561 tgtgaccaga aatcctcagt tgatccaagg actttattaa cgaaatcgat ttgttctctc
     7621 gttgcgctat tatcaacaga acgtgagaca ttctgagatt ggtaactaga aggtgaggat
     7681 gagtcattaa aaatgctggt tagtccccct ccgccaagta gcagtaagat gattaaaacc
     7741 acaagcttgg ttttccaact ccctcgcatc aataaaagtt gaagaatcgg taatccactt
     7801 cctccgctag agaatgaacc tccgctactg gagcgacgat cttcaacatt gtcgcttttt
     7861 cttaggtcat ctattttcat aagtatctcc aaagtgtttt ataggctcat tataaactat
     7921 tttaataaga aatgcttgaa tttttaacta aatatcttcc cctagatttg aagctatttt
     7981 tggtataata ttaataaact gtaaagaata agttgaagaa ggtttatttc tcagttgact
     8041 tataagatag gagattcatg aaattacttt atactgatat taatcatgat atgacagaaa
     8101 tcttagtcaa tcaggccgcg catgctgctg aagctggatg gcgtattttt tatattgctc
     8161 caaactcttt atcttttgaa aaggagcgtg cagtattaga gacccttcct caagaagcct
     8221 cttttgctat tacaattact cgctttgctc aattggcacg ttattttact ttaaaccagc
     8281 ctaatcaaaa agaaagttta aacgatattg gattagcaat gatcttttat cgtgctttag
     8341 caagttttga agatggacaa ttaaaagttt ttggacgatt gaagcaagat gcttccttta
     8401 tcagtcaatt ggtagaccta tacaaagaat tgcagacagc aaatctgagt attttagatt
     8461 taaaatattt acattctcct gaaaaatttg aagatctatt agctattttt ctagtggttt
     8521 cagatctttt aagagaagga gaatacgaca atcaatctaa aattgccttt tttacagaac
     8581 aagtaaggtc aggacagcta gatgtggatt taaagaacac tatccttata gtcgatggtt
     8641 ttacccgttt ctcagctgaa gaagaagctt taattaaaag tctgagttca cgttgtcaag
     8701 agattatcat cggagcttat gctagccaaa aagcgtataa gactaacttt accaatggaa
     8761 atatttattc tgcaggggtt gattttttaa gatatctagc aacgacattt caaacaaaac
     8821 cagaatttat cttgtctaag tgggaaagta agtctggttt tgaaatgatt tcgaaaaata
     8881 tcgaaggtaa acatgatttt acaaatagta gtcacatttt agacgacact gctaaagatt
     8941 gtataacaat ttgggagtgc attaatcaaa aagatgaagt ggaacatgtg gctagagcta
     9001 tacgtcagaa actttatcag ggatatcgct acaaagatat tttagtttta ctaggagatg
     9061 tagatagtta taaactacaa ttaagtaaga tttttgaaca gtatgatatt ccttattatt
     9121 tcggtaaagc tgaaacaatg gctgcgcacc ctttagttca ttttatggat tccttgtcgc
     9181 gaataaaacg ttatcgattt agagctgaag atgttttaaa tctttttaag acaggtattt
     9241 atggagagat tggtcaagat gatttagatt attttgaagc ttatatctca tatgcagata
     9301 ttaaaggtcc taagaagttc tttacagatt ttgttgtggg ggctaaaaaa tttgatttag
     9361 ggcgtcttaa cactattcgt cagtccctcc ttactccact ggaaagtttt gttaaaacga
     9421 aaaaacaaga cggtataaaa acactcaacc aattcatgtt tttccttact caggtaggtt
     9481 tgagtgacaa tttatcgcga ttagttggtc aaatgtctga aaatgaacaa gaaaaacatc
     9541 aagaagtttg gaaaacattt acagatattt tagagcagtt tcaaacaata tttggtcaag
     9601 aaaaattaaa tttagacgaa tttttatcgt tattaaattc aggtatgatg caagccgagt
     9661 atcgtatggt ccctgctact gttgatgtgg ttactgttaa gtcttacgac ttggtagaac
     9721 cacatagtaa tcaatttgtc tatgccctag gcatgacaca atcacatttt ccaaagattg
     9781 ctcaaaacaa atctttaata tctgatattg aacgtcagct tataaatgat gccaatgaca
     9841 ctgatggtca ttttgacatt atgacgcgag aaaatttgaa gaaaaaccat tttgctgctc
     9901 tctctctttt taatgctgct aaacaagcat tagtgttgac tataccacaa ttattgaatg
     9961 agtctgaaga tcagatgtca ccatatcttg tcgaactaag agatataggt gttcctttta
    10021 atcataaagg acgccaatcg ttaaaagaag aagctgataa tattgggaat tacaaagctt
    10081 tattatcgcg tgttgttgac ttgtatcgct ctgctattga caaagagatg actaaagaag
    10141 aacaaacttt ttggtcagtt gctgttcgtt atttgcgtcg tcaattaact tctaaaggga
    10201 tcgagatacc aataattaca gatagtcttg atactgtgac agtttcatca gatgtgatga
    10261 ccagacgctt cccagaagat cacccactta aattatcctc atctgctttg acaacttttt
    10321 ataataatca atataagtat tttttacaat atgtgttagg tttggaagaa caagactcaa
    10381 ttcatccaga tatgcggcac cacggaacgt atcttcatcg tgtttttgaa attctgatga
    10441 aaaaccaagg gatagaatct tttgaagaga aactcaactc tgcaattaat aaaaccaatc
    10501 aagaagatgt tttcaaatcc ttgtattcag aagatgcaga aagtcgttat tctttagaaa
    10561 ttttagaaga tatcgcgcgt gcaactgcaa ccatattacg acaagatagt cagatgactg
    10621 ttgaaagtga agaagaacga tttgaactaa tgattgataa tactataaaa attaacggta
    10681 ttattgaccg tatcgatcgt ttatctgatg gttctttggg tgtcgtggac tataaatcaa
    10741 gcgctcaaaa attcgatatc caaaaatttt ataacggtct aagtccacaa ttagtcacct
    10801 acattgatgc tatcagtcgt gacaaggaag ttgaacaaaa accaccgatt tttggagcta
    10861 tgtacttaca tatgcaagaa cctagacaag acttgtctaa aattaaaaat ttagatgatt
    10921 tagtaacaaa aaatcatcaa gctttaactt ataaaggatt attttcagaa gccgaaaaag
    10981 aattcttagc aaatgggaaa taccatctca aagattccct ttattcagag gctgaaattg
    11041 ctattttaca agcacataat caatcgcttt acaaaaaagc atctgagact attaagtcag
    11101 gtaagttttt gatcaatcca tatacagaag atgctaaaac agttgacggt gatcagttta
    11161 agagtattac ggggttcgag gcggataggc atatggcacg tgccagagcg ctttataaat
    11221 tacctgctaa ggaaaaacgt caaggtttct taacattaat gcagcaagag gaggaaaatg
    11281 atgaccttta agcctttctt aaatccagaa gatattgctg ttattcagac agaagaaaaa
    11341 aattctgata aaaaacaaaa gcgaacgcct gaacaaattg aagcaattta tacttttggt
    11401 aacaatgtcc tggtatcagc ttcggctggt tctggaaaga cttttgtaat ggttgagcgg
    11461 attttagata aacttttaag aggggttccg attgagtctt tgtttatctc aacatttaca
    11521 gtaaaagcgg caggtgagct caaagaacgt ttagaaaaga aaataaacga gtctttgaaa
    11581 tccgcagaat cagatgattt aaaacagttt ctcacccagc aacttgtcgg cattcaaaca
    11641 gctgatattg gtactatgga tgcttttacg caaaaaatag ttaaccaata tggttatact
    11701 ttgggcatat cacctatatt taggatttta caagataaaa atgagcaaga cgtcataaag
    11761 aatgaggtct atgctgattt attttctgac tacatgactg ggaagaatgc agcttcattc
    11821 ataaaattag tcaaaaattt ctcaggtaat cgtaaagata gcaaagcttt tcgagaaatg
    11881 gtttataaag tttatgcttt tagccagtcc atcgataacc ccaagcgctg gatgcaaaca
    11941 gtttttctaa aaggtgcaca aacttacact gattttgaag ctattccaga tcaagaagtt
    12001 tcaagccttt taaatgtaat gcaaacaact gctaatcaat tacgtgattt aacggatcaa
    12061 gaagattata agcaattaac tgctaaaggg gttccgacag caaattacaa aaaacacctt
    12121 aaaataattg aaaatttagt ccattggtct caagacttta atttgctgta tggaaaaaaa
    12181 ggattggcta atttggcacg tgatatcact aatgtgattc cttcaggcaa tgatgttaca
    12241 gttgctggcg ttaaatatcc tatctttaaa caattgcata atcgtatcgt tggacttaag
    12301 cacttagaag ttatttttaa gtatcaagga gaatctcttt tcttgttaga attattgcaa
    12361 tcctttgttt tagatttctc agaacaatat ttacaggaga agattcaaga aaatgctttt
    12421 gaattttctg atattgccca ttttgcaatt caaatattgg aagaaaatca tgatattagg
    12481 caaatttatc aagataagta tcatgaagta atggttgatg agtatcagga caataaccat
    12541 acccaagaga gaatattaga attattgtct aatggacata atcgtttcat ggttggagac
    12601 ataaaacaat caatttaccg ttttcgtcag gctgatccac aaatttttaa tgataaatac
    12661 aaagcttacc aagacaatcc aagtcaaggg aaattaatta ttctgaaaga gaattttcgg
    12721 agtcaatcag aggttttaga cagtacaaat agtgtgttta cacatcttat ggatgaagaa
    12781 gtaggagaca ttctttatga tgaaagtcac caattaaaag ctggtagtcc aagacagcaa
    12841 gaaagacatc cgaataataa aacacaagtt ttactattag atactgatga agatgatata
    12901 gatgacagtg atagtcaaca atatgatatt agcccagctg aagcaaagtt agtcgcaaaa
    12961 gaaattattc gattgcataa agaagagaat gttccctttc aagatatcac cctcttagtt
    13021 tcttcaagga caaggaatga tggtatctta caaacttttg accgttatgg tattcctttg
    13081 gtaactgacg gtggggagca aaattactta aaatcagtcg aagtgatggt aatgcttgat
    13141 actttgcgat caattgataa tcccttaaat gattatgctc tagttgctct tttgagatct
    13201 cccatgtttg gctttaatga agatgattta acaagaattg ctattcatga tgtcaaaatg
    13261 gctttttatc ataaagtgaa attgtcttat cataaagaag gtcatcatag tgatttaatt
    13321 actccagaat tatcttctaa aatagattac tttatgaaga cattccagac ttggcgtgat
    13381 tttgctaaat ggcattcgct ttatgattta atatggaaaa ta
//
LOCUS       DATROY010000046        13295 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.047, whole genome shotgun sequence.
ACCESSION   DATROY010000046 DATROY010000000
VERSION     DATROY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13295
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1207)
                     /locus_tag="VBD11_001047"
     CDS             complement(<1..1207)
                     /locus_tag="VBD11_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO6954007.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKA"
     gene            complement(1217..2290)
                     /gene="purK"
                     /locus_tag="VBD11_001048"
     CDS             complement(1217..2290)
                     /gene="purK"
                     /locus_tag="VBD11_001048"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6954008.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTAFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            complement(2277..2765)
                     /gene="purE"
                     /locus_tag="VBD11_001049"
     CDS             complement(2277..2765)
                     /gene="purE"
                     /locus_tag="VBD11_001049"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6954009.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(3051..4313)
                     /gene="purD"
                     /locus_tag="VBD11_001050"
     CDS             complement(3051..4313)
                     /gene="purD"
                     /locus_tag="VBD11_001050"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6954010.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            4595..5398
                     /locus_tag="VBD11_001051"
     CDS             4595..5398
                     /locus_tag="VBD11_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6954011.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(5421..6398)
                     /locus_tag="VBD11_001052"
     CDS             complement(5421..6398)
                     /locus_tag="VBD11_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6954012.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(6406..7287)
                     /locus_tag="VBD11_001053"
     CDS             complement(6406..7287)
                     /locus_tag="VBD11_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6954013.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(7304..8221)
                     /locus_tag="VBD11_001054"
     CDS             complement(7304..8221)
                     /locus_tag="VBD11_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6954014.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(8218..8880)
                     /locus_tag="VBD11_001055"
     CDS             complement(8218..8880)
                     /locus_tag="VBD11_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6954015.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(8900..9343)
                     /locus_tag="VBD11_001056"
     CDS             complement(8900..9343)
                     /locus_tag="VBD11_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6954016.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(9356..10186)
                     /locus_tag="VBD11_001057"
     CDS             complement(9356..10186)
                     /locus_tag="VBD11_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6954017.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(10196..11083)
                     /locus_tag="VBD11_001058"
     CDS             complement(10196..11083)
                     /locus_tag="VBD11_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6954018.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(11171..12487)
                     /locus_tag="VBD11_001059"
     CDS             complement(11171..12487)
                     /locus_tag="VBD11_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6954019.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(12534..13232)
                     /locus_tag="VBD11_001060"
     CDS             complement(12534..13232)
                     /locus_tag="VBD11_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6954020.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
BASE COUNT         4037 a         2784 c         2252 g         4222 t
ORIGIN      
        1 ctgctttttc agtcaactcg atagcaagtt ctttatgctc ctctaacact gctttgatgt
       61 cagaaaatgg gactttaaaa tactcttttc gcccattaac tttattaatt ttattttttt
      121 caaatctgtt atgtaactct ttttctaatg aaaaagcttc ctcactaaaa attaaggcat
      181 gtacatcaaa ttgaaatgga acagatgcac tccctaattc gcgaatacgt tccataggat
      241 ctagacgcct tgtaacacca atcttgtaaa catcttctcc aaatgcacca atatttgaaa
      301 tgatataaac atatccagca gttgcatgcc cttctcgata atcaatatct tcttctaact
      361 catctagttc tgataattta tcttggtatt ctgctaattg aagatttaat ttttcctttt
      421 cttcctcatt agctgtttta agtaattctt caacgttatc taacatctgt ttaaagtgtt
      481 ttctatcttt ttcaatttgt tttctcttag cagctaattc tgcttgaagt ttcttatctt
      541 ctctttcctt cgcacgttgt tcacgaagaa gttctttttc ttctaatttc tgaagttcat
      601 attctactgc taattgtaac tctgaaattt ttagctcaag atagtcatta gatagacata
      661 ccaaatttct tgaatacatc ttattaagct gattaaatga tttaacaact gcatctactt
      721 ttttacttag attagtagcc gttaccttta ccaggtgagc atcaacttct ccattaaaac
      781 ctcttatcat cgctttaata agttgatttt gcattgctcg tccttttgat ttactgttat
      841 ccagcaacat aggtcttaca ataacacccg cttttttatc ttttactaac tgtttttgcc
      901 tctttctcag agtttccaat tcttctttat acttagtact atctgagaac ttatattttc
      961 tctcaaaaaa tccgtactct tgaagatcta aatcatcatt aagaactatt acctcttttt
     1021 gtaaagcttt aagttctgat tggcgtttct taagttcatc atcaagtttt aaaatatctt
     1081 tagcaacctt tatctgcctt gttaaatcat ccaaaataga ttgtttatct tttaacttac
     1141 tttctaaact actgatttta gcttccaaag cctcatcttt aggctttccg aatccaaata
     1201 gacccattta ttccccctaa aactcctcca cctcatccgc atcttccaca aacaccgtta
     1261 catgtcccat tttgcggtta tgtttcgctt ctagtttacc atacatgtgg aggtgagcgc
     1321 tagggttttg ggcaacatag tcaatcgcct gctggacatg ttgtccaagg acattaagca
     1381 taacggctgg gtcatggagc ttgattggtg ggagaggttg ccccagtaca cccaagatgt
     1441 gggtgtcaaa ctgtgaaaag tcacaggctt cgatagaata gtgccctgag ttgtgggggc
     1501 gtggggcgat ttcattgacg atgatatcat ctgcggtcac aaacatttcc acacagaggg
     1561 ttcctgatag ctggagttcc ttggcaatct gtaccgccat tttcttagcc ttgtcagcta
     1621 gttggtctga gatgcgagct ggtacgatag tttttgatag gatattgttg cggtggatat
     1681 tttcctgaac gggaaaggcc gtcacatcct tgccatttcc agacacgatg actgagattt
     1741 caaggtcaaa gtcgacaaac tcttccaaga cacactcagc tgagttggct aattgttgcg
     1801 cctctggcag gtcttctgct gagcggataa ccttttgccc atgaccgtca taaccacctg
     1861 tcgctgtctt gaggacatag gtcttggtca agtcaagccc ctctaggtca aggctagacg
     1921 tcaccacctt atagggagcg acagtcactc cagccttatt tgccagaaag tctttttcaa
     1981 agatacggtt ttgagaaatg cggagcagat cagtcccctg cggtagctga ccagctgaca
     2041 caacggcatc cagaccatcg gcatcaacat tttcaaactc ataggtcaaa acatcgcaac
     2101 gcgcagctaa ttgtcccaaa gcctcaacgt catcgtaagg cgccacaatc acctcgctaa
     2161 cgcgggaggc agggcagtcg ctagcaggat ccagcgtaat gaccttgtgg cccatgtaga
     2221 tagccgcaat cgccatcatc tgccccagct gaccaccacc gataatccca atggtcttaa
     2281 atgagttcat tgcttgactc ctctgcgatt tttccttgtt cctcatggaa atgagctagc
     2341 gcatcagcca gattttggtc ctcgatagag agaatacgga gggctgttag ggcagcgtta
     2401 gtcgctcccg cttctccgat agccatggtt gccacaggca caccgccagg catctgcaca
     2461 atggaataga gcgaatccaa gcctgaaagg gcacgtgatt tgacaggcac accgataaca
     2521 ggcagggttg ttttagctgc taccatacca ggcaagtggg ctgccccacc tgctccagca
     2581 atgatgattt tgataccacg accacgcgcc tcctcagcat gcttgaacat gaggtctggc
     2641 gtacggtggg cagagacgac tttcttctcg taagcaatgc caaagttatc tagaacttca
     2701 gccgtttttt gcatggttgt ccagtcggat ttggaaccca tgataataga aataattggt
     2761 tgcataatat taagatacaa agggacgttt cgacttgtcg aaaattttcg tagaaaaata
     2821 tgcggtaagt ccgaaatctt tgatttcgaa gacgcaccca cgtcagggcg actaggtgct
     2881 ttagcagcag acgcctacga cgcaaaaatc tgacgaagat gctttagcat ctaggaagat
     2941 ttatcttttt cactagaaaa ttagtcccgt gttcagttcc tttcttcttt ctttttggag
     3001 cgaccttcgg tcgctgattg ctgacgctaa tatttgaatg agtagtctta ttacttaata
     3061 gctttgcttc cgatgtcgtt tcggtagaag aggcctgtgg tgtcttgttg ggctagttgg
     3121 gtatagattt tgtcctgccc tgctttgacg ctgtcttctg tggtgacaag catatagact
     3181 cgtccaccgt ttgagagcag tgctttgcca ttttccgcaa acttagctcc ggcatagtag
     3241 gtgatgatgt cgccgtcggt cttttcaggc agtggcacac ctttctcgta atcgagcgga
     3301 tagccttctg aggcaacgac aacgcccaaa gtcacgccgt ccttctgcca agtgatgtaa
     3361 ggctcgatgc ccatcatgat gtcgtcgatg ttctgagcaa aatcagaagt caggcgaggg
     3421 aggataatct gagtttctgg gtcaccgaaa cgtgagttga actcgataac cttcggacca
     3481 tcagccgtca ggataagccc cgcatagagg acgcctagat aaggacgccc ttcagtaatc
     3541 ataccctcaa ggataggctt aacgatggtt tcaaccgccg tatccactac gctctgaggc
     3601 aggtggggaa ctggcgcata ggcacccata ccgccagtgt ttggtcccat atcaccgtca
     3661 taggcacact tgtgatcctg agctgtcggc atgatgtaaa acttgtcgcc attagcgaag
     3721 gcaaagaggg agaactcttc accatccaaa aattcctcga taaccacacg cgctcctgag
     3781 tcgccaaact tgttgtccaa aagcatctct tgcgccgcct ctaccgcctg ctcaacggtt
     3841 tcagccacga ccacgccctt gcctaacgcc aagccatcag ccttgaccac gattggtgca
     3901 ccctgctctt cgatgtaggc tttagctttt tcaaaatctg aatatgtgcc ataggctgct
     3961 gttggaacgt tgtatttaac catgatttcc ttagcaaagt cttttgacca ctctagctcc
     4021 gctgctgcct ttgttggccc aaaagctcta agtccagcac tgttgaaatc atcaacgata
     4081 ccagccgcta gcgcatcatc aggaccaata aaagtccaag caatctcatt ctccttagca
     4141 aagtcaatca gcctagaatg ttcggaaatt acgatattta ccaagcctag accatccaag
     4201 gtcataccat cattaccagg tgccacaaaa acctgatcta cacccttaga cactaacaac
     4261 ttcttagcaa tcgcatgctc acgaccacca gaaccaacaa caagcaattt catatcagaa
     4321 taactcctat taaataatat aaaaagaaat ttgcaataaa aacgtattat ttataaaatt
     4381 ataacacatt tgttcgcttt taagtttttt gttgcatctt ttttgaaaaa ttaataacat
     4441 aatatatact tcacatatat tatgttatta atccttcata ttgctattta aaacttatca
     4501 ttgctgtttc ttagaactta gtttggcccc tcgctaactt tatgttatac tgaatatgtt
     4561 aagcgctttc ttaaagagga gaggaccgtg cattatgact atttatgacc aaattgagtc
     4621 tgctcttgat ttaatgactg atttagaaca tgaaattgct tgttatttta tggggcagcc
     4681 catttcaaaa gacgctcttg cttcttccat tgtcactaag caactacaca tttcacaagc
     4741 agccttaacg cgttttgcta aaaaatgtgg gtttaaaggc tatcgtgaat ttgtttttga
     4801 atatttaaaa agccatgaga ccatcagtca acaattatac ggtcttcaaa atgacaacac
     4861 taagaaagta tttatgaatt atcaagagat ggtttctaaa tccgctgata ttattgatga
     4921 agaacagctt ctagaagtat cacatatgat tgagcaagct gatcgtgttt atttttatgg
     4981 caaagggagc tcatcgctag tagctaaaga atttaaaata cgccttatga ggctgggagt
     5041 gatttgtgag gctctggatg atacagatag tttctcatgg actaatagta ttgttaatga
     5101 tagatgcctc gtcattgcct tctcactttc tgggaacacc aactctgtta tcggtgcctt
     5161 aaaaatcgca agttgtcacg gagctaaaac cgttctattt acaaaacaac ctcacacaat
     5221 tgactatgct tttgacaaga ttattcaggt ggcctctgct aggcatttag actacggcaa
     5281 tagaatttcg cctcagatcc caatgttaat catggtagat attatctatg cacaattttt
     5341 agatattaat aaaatagaaa aagagcgaat ttttagagaa accattattc aacgctaaat
     5401 gaaaatcgag tagaaataat ctactcgatt ttttgctttg taaattgact tcctgtcagt
     5461 tgatcaaaaa tatagtcttt tacttgtact gtcattggat catgtccata gtctggtaat
     5521 aaaagatgct ttttagttga tgtcaaacgg ttaaaaatgg caaattgact tgatggagga
     5581 caaatatcat ctttcaaggc tgttagtaaa acaacggggc aagaaatcct atgtgcaaaa
     5641 tttttaacat caatataagc caaagttttt aacacattat tttctgtttt atgaaaagga
     5701 tcactatatt taaaatagcg gaagagttcg tcataaggct cactgacatt ccctaaatct
     5761 aatactcgtc taaagtctga caaaaatggg taaacagcaa cggtcttgac gattttggga
     5821 ttaagagctg cagctatcaa agcaagtgct ccaccttgag accagccata actataaagt
     5881 tgattactat caacactctc taatgtagca atgatatcaa tcaattggaa aacatcaaga
     5941 taaatgtctt tataaaaaag atgatttggt cctgagatca ttcctcgcac aatctggccc
     6001 ttaactgtaa tgccatcaaa atgccccttg tcttgagact gtccagcttg acctcgaaca
     6061 tccattgaaa caacgccgta acctgctgct acataattaa gttggtcact ccagtcagga
     6121 ctttggtttt ggtaaccatg aaaataaaaa actacaggat agggtttatt tgttttaggg
     6181 aataaacatt tggcatagac aatagagtta ttgctgcctt taaaatgaag atcataagct
     6241 tgtgcaaaag taatattgaa atcttttttg tctagacgat actcaatatt cccttgatat
     6301 ttcattgttt gttttttcca gaagtcatca aaatcctcgg gaatttggtc ttgacctagg
     6361 tattctctca tgtcatctag cgacattgtt tcaatcatat acccgtcacc ccctgttttt
     6421 aaaatggtag taagctccta gcataccagc ttggttttcg tgttgtgcaa aaactattat
     6481 tgttttttca gcaagacttg tcacaagatt tcgtttcaaa gattcactaa gtttatcttg
     6541 taaataatct ttttgagcca tgattccacc acctaaaaca actttttcag gatttaccac
     6601 ataaaccata ttagcgattc cttgaccaag gtagtttatc atacggtcta tacttgcaat
     6661 acatttctca ttgccttctt tagcctcctg aaaaatacga cgtccatccc atcggttaat
     6721 ttcatctcca tgcgctttgg caacatcagc aatcagtgca gttgtagagg ctaaatcttg
     6781 aaaatcacca tctgaaaggt gcatatagcc aacttcacaa gcagaattac taaagccatg
     6841 aaaaactgtt ttatcaataa tcagacaacc accaatacct gttccaatag taagacaaag
     6901 cgctattgaa ctatctttgg cactacctga gacagcttcc gctaatcctg cacaattaac
     6961 atcattttca atttctgtgc gtacttgata agtgtcttct aaaactttct taaattgtgt
     7021 cccagcatag tttggaattt gaggaccaga gtaaaatatg catccttcat ctggatctac
     7081 catgccagca gatgagatag caattccatc aatgctccct tctgttagat agttatcgat
     7141 aatttgacaa actttttgta aaatgcctgg accacctttg taggcttctg tggcaagctc
     7201 acttgcctca acaatacaac ctaaattatc cacaatgcca tgcttaatca ttgtaccgcc
     7261 aatatcaatt gctactgttc tggtcataat tatctacctc actttaacaa aactgttttt
     7321 tagcatgtct aatcatttct gctgcctctt taataatagg tttatcttct tctgttacag
     7381 atgctaaagg taaccgaacg gaacctatag ttagttgctc attgatttcc aatacagctt
     7441 tgatcacagc atacatatta ccatgacccg aacaaagctt tgtgataata tcgttaattg
     7501 taaattgaag ttcacgcgct ttttctaaat ctttatcaac aattaactga tttaaagtca
     7561 agtataattc tggcattacg ccataggtac caccaatacc tgcagctgct cccatgagac
     7621 gaccacccaa aaattgttca tctggaccat taaaaacaat atgattttct cctccaatag
     7681 ccacaaaatt ctgaatgtct tgaactggca tggaagaatt tttaacacca attacttgcg
     7741 gattttgtaa catttttcga tataaatccg atgtcaaagc aacccctgcc aattgaggaa
     7801 tattgtaaat aataaaatca gtttgtggtg ctgcttgact aatagtattc caataatctg
     7861 ctatggcata ctctggtaat cgaaaataaa ttggtggaat tgctgcaata gcatcaacac
     7921 ccatagcttc agcatgcata gccaactcga cgctatcctt agtattatta catgctacat
     7981 gcgctatcac tgttagttta cccttagcaa cggacataac attttccagt acaagtttac
     8041 gatctgctac actttggtaa atacattctc ctgatgaacc attaacataa agtccttgaa
     8101 cacctttatc aataaaataa ttggtcaggg cttttactcg ttcgggacaa atatcaccct
     8161 tatcatcata gcaagcataa aatgcaggaa taattccttg atatttttgt aaatctttca
     8221 ttgataatcc ccttgttgtt tgctgaaata ggcaaattgt ttttgtaaaa taataatttt
     8281 aaagtaagca aaggcggtat aagcaaaaat aaatatagct gacactgtaa gcataaataa
     8341 gagactagac tgcaccataa taaaccaagt catgattaat aaaaacatca atacaccagt
     8401 ccatttcaag tttaaacatg ccattataaa gctctttttc acggtatcta aaagagacat
     8461 ttcatagcgg gcagccatag gaaataggta tatgctcgtc atcacagtca ataacaaaaa
     8521 ggcgtaacaa attatcttca agaaaggcac aatgaaacct atttgataat gaagaattgt
     8581 taaatcaaat atgatcacaa ccaaaatact tagttcaacc aaacctaaaa gcataccttg
     8641 gaaccactct ttttttaggt gttttatata caatattaaa agatttgtct gatcaccttc
     8701 taatttttgc cacaacgttc gatacaaact aattttagca gcaccaatag ttaccaatgg
     8761 taggcaagtt atcataaaga gaaggtttag caatgccaga tcaaaaacaa cattgcaacc
     8821 ttgcataaag gggttgttaa gatcaaaaat cgctgctatt aattgatttg cttttttcac
     8881 aatatactcc ctgtatttct tacgccatta acactttcaa tacccctttt ttgacataat
     8941 tacctagact tcccatacca tttggctgat gagcttcttc tgggaaaaag aaggctatat
     9001 aattatctct caaatcaaaa gaagtctctt ctgagcactt gacaaatcct atatcgccta
     9061 agtattcctc tacctctacc ttatcacctt ttcccaattt gataacttca tatccttcaa
     9121 tgacataatg tatatcaagg tattttttat ggtattcaaa aatatgatca aatgactcac
     9181 taagttgatt tgattggacc atgagtatca cttctgggct aatatgatag gtgcctatat
     9241 ctaaattcct taaatcatga cttagtagat aatcaatcgc taaatctaaa ttaggattaa
     9301 tatctttata atgcgttaaa ttcaataaat gatcgtaaat cataataaat cctacctatc
     9361 ctttaacagc tcccattgta atcccctgag taaaggattt ttggaaaact aagaatactg
     9421 tcacaatagg aacagcagct aaggctgcac ctgccataat taagccatag tttgttgcca
     9481 tttcagcctg catagtagct accccaagag aaatggtcag attatttcta gaagttagca
     9541 taactaattg cataaaatag tcattccaag tattgataaa tgtgaaaata gctagggcag
     9601 caaatccagg tttgacaatt ggaaatgcta cattaataaa tgttcgaatc tctccacaac
     9661 catcaatttt agctgattcc agtaattctg ttggaatatt ttcagaaaac tgtttcatca
     9721 agaagacccc aaaaggccaa ccaacaagag gtaaaataac cgcccataag gtatcatgta
     9781 tccccataaa attgataatt ctaactaatg gcactaaaac aacttgttta ggtaaagcca
     9841 tagcagcaat aaaaagggag aacaatattt tttgaccgta aaatcgtttc ttagctaaaa
     9901 catagcctgc cattgacgat gtacagcaaa ctaggaacat tgtcataatt gagataaaga
     9961 cactattcca aagccatctc aaagctgggt tttgtacagt taaagcttta aaattttcta
    10021 aggtcggtgc tttgggccac cattgtgggg ggatgataat agtatcaggt tgagatttaa
    10081 aagctcctgt cataatccaa taaaatggaa agataaaaag cactgtcaaa agacaaagga
    10141 taagagccgt taaaaagtta taagcactga atgttttctt tttcatgttt tcctcctaat
    10201 attccacatc atttcctaag atcttaaatt gagcaaaact tattaaagca atcattactg
    10261 ctaggaatac tcccatagta ttagcatagc catattctga tagtttaaag gctttttcat
    10321 aaagataata catcaaagtg cttgttgaat aatttggtcc accagatgtt aggagctgga
    10381 ttaaggcaaa acattgaaat gaattaattg ttgttataac tgcaatatag agtgtagttg
    10441 gtaagagact cggccattta atttgccaaa aaacttgcat ttcattggct ccatcaacac
    10501 gcgcagcttc gcaaagagaa ttatcaatat ttcccatcgc cgcaatatat agaataatag
    10561 gttgaccaac tgaggtcgtt agcaaaataa taataatagc aagtaatgcc cagtgcttat
    10621 caccaagcca gctaatattc tgttcaatga catgacctga tttcaagatg taatttaaaa
    10681 ttccggacat agggtcataa atccatttcc aaacaactgt gactgcaaca ctaccagtta
    10741 ctactggcaa gaagaagaca caacggtaga aagagcgtga aaaaacattt ttttcataag
    10801 tattggcagc aacaaaaagt gagaagaaaa ccactactgg cacagagcca atcacaatga
    10861 ttactgtatt tatcaaggat ttcatgaaaa tagaatcatg aaacatacga ttgtaattag
    10921 ctaacccaat aaaagtgaac tgtgtcatag aataattaaa aaaactagtg acaaagccca
    10981 taaccatcgg tgcaaaaaca aaaattaaga aaaataagag aataggcgct agaaaagcat
    11041 aagctatcat cgtttccctc atctttaact tattagtacg catacggtac tctcctttac
    11101 atcttaactt gactaaaaac tcctcttcta tcccaatatg tgattgacca tagaaaagga
    11161 gtctccttaa ttatttagct gcttttttaa tggtatcatt tgctttttga gtaaagtctt
    11221 tcaaagcatc tgctggtttt tcatcaccat tggatacaga ttgaaccatt gggaaccata
    11281 aggttctcat ttcagaaaat ccatcgatag tgttgtaata tggtgaataa tattgagtcc
    11341 attttgaaat cttcatcata cgtttatcac ctttataaag atccccaaat gatgttctaa
    11401 ctgggaaagc acctgtacgt ataacatctt ttggtcccca ttttttatca tcagcaataa
    11461 aagtgataaa tttcttagag gcttttactt tgttttcatc tttattatta aagaccgcaa
    11521 aaccattaac aaggtattct aaatctggtt ttccatcttc tgatgggaat ggcacttcaa
    11581 ggtaatccac tttacttgac tctaataatt ttgcttgagt ttttggttga gctggagccc
    11641 ataggatagt gaaagcagtt tgtccattgg cgaagttttg aatgtcagct gagccatcat
    11701 actgagaccc attcatcaag tagcctttct taatccattc aactattttt ttcattgatt
    11761 ttacagaatt tttagtgtca gtggtatatt ttgttacttc tttatctgtt attggagcac
    11821 tataaagatt agcaaaaaat gcacgtggtc cttgatctcc tccttgcccg tttgcaaaga
    11881 atgaacctgg tgtatagcct ttatttttta gtgcttttag tactttttca aaatcactag
    11941 tagtccaacc ttcttttaca agtttcaaaa ctcctgcatc tttaagcatt tttttattga
    12001 acgccatata aaatggggca gaacttattg gatacatgta ggctttatcg ccagacttag
    12061 aagcttgaat gatgttctta ttattgacat ccttaataaa ttggtctgta aataaatcat
    12121 tcaaatctgc taatttacca tttttaccat attgaataat tcgccctggt gcatcaaaaa
    12181 gcacatcagg tgctgtccct gcttcaattg ctgtagtgat tttttcaggt ccagatgtga
    12241 aatcaattgt ctctagtttt acttttatat taggattttt cttttcaaaa gccttgatga
    12301 cttttttctc ataagtacct actccatcct tagccttttc ttgagtaaag gttggaaaag
    12361 cccaccacgt aatttcgact ttatccttct cggcagccat aacagattga ctactgtcta
    12421 cacaagcaaa cattgataat gctgcagctc cgaggcaaaa accaatcaca ctttttttga
    12481 tactcataac tattcccctt ttttattttt attttgattt ttgagtaaca cttttaacta
    12541 agtcctgaga tgaaacgctc cgctatttct tttggtctag tgatagcacc cccaactaca
    12601 attcctgcaa cacctatatg attaatttca ttagcctgct taggagtatg aattttacct
    12661 tccgcaatca catctatacc ggcttgacaa agcttattaa ggagttctat atccggtcct
    12721 tcttcttggc ggctgtaatc tgtgtatccc gatagagttg tacccacaaa atcaactcct
    12781 gcttcaaaag catttttgcc ttcttcaaaa gtacttatat cagccattaa caactgttta
    12841 ggatatttcc tttttatctt ttgaataaac tcagctacac tcaaaccatc atgacgctct
    12901 ctaagtgtac aatctaaggc tattactgca atatctaaag tagctaattg atccacctct
    12961 gtcatcgtag cagtgataaa tggttcttgt ggaggatatt cacgtttaat aatgccgatg
    13021 ataggtaaat tagtaacttc ttgaatttcc ttaatgtcgc ggacactatt ggctcttata
    13081 ccaaccgctc ctgcttcttg agctgccaaa gctaaaagag gcataacacc tccactttca
    13141 gtataaagag gctccccagg caaagcttga catgacacaa taatgccatt ttttatttgc
    13201 tttttaaaag cttctttact tagatgtggc atattctcac ttccttatct tttttaattt
    13261 tagatatcta caaaccttat tatatctatt tttaa
//
LOCUS       DATROY010000047        13004 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.048, whole genome shotgun sequence.
ACCESSION   DATROY010000047 DATROY010000000
VERSION     DATROY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            277..666
                     /locus_tag="VBD11_001061"
     CDS             277..666
                     /locus_tag="VBD11_001061"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6954021.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            731..1780
                     /locus_tag="VBD11_001062"
     CDS             731..1780
                     /locus_tag="VBD11_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6954022.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            1925..3460
                     /locus_tag="VBD11_001063"
     CDS             1925..3460
                     /locus_tag="VBD11_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954023.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3453..4514
                     /locus_tag="VBD11_001064"
     CDS             3453..4514
                     /locus_tag="VBD11_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954024.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFQLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4516..5472
                     /locus_tag="VBD11_001065"
     CDS             4516..5472
                     /locus_tag="VBD11_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954025.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            5677..7047
                     /locus_tag="VBD11_001066"
     CDS             5677..7047
                     /locus_tag="VBD11_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6954026.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(7168..8157)
                     /locus_tag="VBD11_001067"
     CDS             complement(7168..8157)
                     /locus_tag="VBD11_001067"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6954027.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            8396..10855
                     /gene="gyrA"
                     /locus_tag="VBD11_001068"
     CDS             8396..10855
                     /gene="gyrA"
                     /locus_tag="VBD11_001068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6954028.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            10862..11605
                     /locus_tag="VBD11_001069"
     CDS             10862..11605
                     /locus_tag="VBD11_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6954029.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            11621..12034
                     /locus_tag="VBD11_001070"
     CDS             11621..12034
                     /locus_tag="VBD11_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6954030.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            12187..>13004
                     /locus_tag="VBD11_001071"
     CDS             12187..>13004
                     /locus_tag="VBD11_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6954031.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNL"
BASE COUNT         4208 a         2000 c         2688 g         4108 t
ORIGIN      
        1 cgtattattt tttggaaagg ttttctctaa aaatgttttc tttaaattcc gtataagata
       61 aaaagaattc tgctttttat tctgaaaatt ataaaaaact acttacatta tctaaaaaaa
      121 atggtaaaat agattcagct aaaataccaa gactaatgaa actacaagac ttggttactc
      181 aataataaat acttctgtgt ttgataagaa gataataaac gtgttctaaa aattttgcca
      241 gtaaacatta tgaagcgaaa ggtgcagcta ctaaaaatgg gaaatataga gttgaaaaaa
      301 ttggctgtaa aagcgagtga aaatgcatac gttccttact cgaaatttcc agtaggcgca
      361 gcattgcgta cagcagaagg aaaaatattt acaggatgta acgttgaaaa tatcagttat
      421 ggattagcaa actgtgctga gcgtactgca atttttaaag ctgtttcaga aggttataaa
      481 gatttttcgg aaattgctat ttatggaaat actgaagaac caatttctcc ttgcggtgca
      541 tgccgtcaag tgatggtgga attttttaat aagaatgcta aagttacatt gattgctaaa
      601 aatggtaaga cggtcgagac gacggtcggt gagttgttac catactcgtt tgttgattta
      661 aaataattta aatcagttgc tcttttaagc atttagctta aatattaagt tttttaggag
      721 ggttcatttc atgaacaaaa aaattgccgg gatcggcttg gcttcgattg cagtacttag
      781 tttagctgca tgtggacatc gtggtgcttc taaatctggt ggtaaatcag atagcttgaa
      841 ggttgcaatg gtaacagata ccggtggtgt tgatgataaa tcatttaacc aatctggttg
      901 ggaaggtatg caagcttggg gcaagaagaa tggccttaaa aaaggagctg gttttgacta
      961 tttccaatcg gcaagtgaat ctgattatgc aactaactta gatacagctg tgtctagtgg
     1021 ttataaattg attttcggta ttggattttc tcttcatgat gctattgata aagcagcaga
     1081 caataacaaa gatgttaatt acgtcatcgt tgatgatgtt attaaaggga aagataatgt
     1141 tgcaagtgtt gtctttgcgg ataatgaatc agcttactta gcaggtattg cagccgctaa
     1201 aactaccaaa acaaaaacag ttggctttgt aggtggtatg gaatctgagg ttattacccg
     1261 ttttgaaaaa ggttttgaag caggtgtcaa atcagttgat aaatcaatta aaattaaagt
     1321 tgactatgct ggttcattcg gtgatgctgc taagggtaag acaattgcag ccgcacaata
     1381 tgcttctggc gcagatattg tttatcaagt agctggtgga actggtgcag gtgtattcag
     1441 tgaagcaaaa tcacgcaatg aatctttaaa agaagctgac aaagtttggg tattaggtgt
     1501 agaccgtgac caagcagcag aaggtaagta tacttctaaa gatggtaagg cttctaactt
     1561 tgttttggct tcatcaatta aagaagttgg aaaatcagtt gaattgattg ctactaaaac
     1621 aagtaaaggt aaattcccag gtggtaatgt aacaacttat ggtttgaagg atggtggagt
     1681 tgatatagca actacaaacc tttcagatga cgccgttaaa gcgattaaag aggctaaagc
     1741 taaaattatt tcaggagata tcaaagttcc tagcaaataa tatctttaag aactttcaaa
     1801 aaagcgatta gaaatcgctt ttttgaagct gagataaact tatatctcag taaaatccat
     1861 cagtcgttaa cgattggtgg attttactga actataaatg aatttttctg aaagggagaa
     1921 ccacatgaca cagaatgtca ttgaaatgaa agaaatcact aagaaattcg gtgattttgt
     1981 ggcaaatgat catatcaatt tgacggttga aaaaggtgag atacatgctc tgcttggcga
     2041 aaacggtgca gggaaatcaa ctttaatgaa catgttagca ggtttactag aaccaacaga
     2101 cggtcaaatt tttattaatg gtcagcctgt aactattgat tcaccttcca aatcgtcaca
     2161 attggggatt ggtatggtac atcagcattt catgcttgtt gaagcattta ctgtagctga
     2221 aaatattgtt ctaggaaatg aaaccaccca aaatggtgtt ttagacatta agacagcagc
     2281 taaagaaata aaggaactat cagagaaata tggtttgtca gtaaatccaa atgctaaaat
     2341 atctgatatt tctgtaggag cacagcagcg tgttgaaatt ttgaagacac tttatcgcgg
     2401 tgcagatatt ttaatttttg atgagccgac ggcggtgctt acaccatcag aaataaaaga
     2461 attgatgaca attatgaaga gccttgttaa agaaggcaaa tccattatac taattaccca
     2521 taaattggat gaaattcggg cagttgctga taaagttact gtgattcgcc gtggtaaaag
     2581 tattgaaaca gtccctgttg caggcgcaag ttctcaacaa ctggcggaaa tgatggttgg
     2641 acgatctgtt tccttcagaa ctgaaaaaaa ggaagctaat ccgactgaca taatcctttc
     2701 tgtaaaggat ttagttgtag aagaaaatag aggcggcgtt ctagctgtta aaaatctgtc
     2761 gttagatgtt cgagcaggtg aaattgtagg tatagctggt attgatggta atggacaaag
     2821 tgaattaatt caagctatta caggtttaag aaaggtaact tctggtcaaa ttgtaattaa
     2881 aggtaaagat gtcacaaaat tttcaagtcg acaaattaca gaattaagtg taggtcatgt
     2941 tccagaagat cgtcatagag atggtttggt tctagatatg acgatggcag aaaatttagc
     3001 tttacaaact tattataaag agccattgag tcataaaggt atccttaatt ttgctaaaat
     3061 taaagagtat gctcgtcaat taatgacaga atttgatgtg cgtggtgctg gtgaacatgt
     3121 tttagcaaga ggattttctg gtggtaacca acaaaaagct attattgctc gtgaagtgga
     3181 tcgcgatcca gacctactca ttgttagcca accgactcgt ggtctagatg ttggtgctat
     3241 cgagtatatt cataaacgtt tgattgaaga gcgcgataaa gggaaagcgg tcttagtagt
     3301 tagctttgag ttagatgaaa tcttaaactt atcagatcgt attgcagtta ttcatgatgg
     3361 taaaattcaa ggaattgtta aacccgacca aaccaacaag caagaattgg gtattttgat
     3421 ggctggtgga aaaattgaaa aggaggagag agatgtctaa gaaggcgcaa aaaattgcag
     3481 ttcctctgat ttcagttgta ctaggaatca ttctaggagc tattattatg ctgatatttg
     3541 gttatgatcc actttggggt tatgagggat tgttccaaac agcattcggt agtataaaaa
     3601 atattggtga aattttccgt gcaatgggac cattaatttt aattgctcta ggtttctcag
     3661 ttgccagtcg tgcaggtttc tttaatattg gacttccagg gcaagcctta tcaggatgga
     3721 ttgccgcagg ttggttcgct ctttcacacc cagatatgcc acgtccagca atgattcttt
     3781 gtactattat tattggtatc gttgctgggg gaatcactgg agctattcca ggtattctca
     3841 gagcatacct tggaacaagt gaagttattg taactattat gatgaactat attgttcttt
     3901 attcaggaaa tgctattgta caacgtgttt tcccaaaaag cattatgcgt acaagtgact
     3961 cgagtgtata tgtttctgcc aatgcatcat accagacaga ttggttatct agtttaacga
     4021 ataattcacg tatcaatatt ggtatcttca ttgctattat tgcagttgtg ttagtttggt
     4081 tccaattaaa taaaacaacg cttggatttg aaattcgttc agttggttta aatccaaatg
     4141 ctagtgagta cgctggtatg tctgcaaagc gtacaattat tctatcaatg attatttctg
     4201 gagcttttgc aggacttggt ggtgttgttg aaggactagg tacatttgag aatgtatttg
     4261 tacagccatc ctcgttagct attggctttg acggtatggc agtaagtcta ctcgcagcga
     4321 atagtccaat aggtatttta tttgcagcat ttctatttgg tgttctaagt gttggtgcgc
     4381 ctggtatgaa cattgcaggt attccacctg agttaattaa agttgtaaca gcttcaatca
     4441 tcttcttcgt aggtgtccat tacattattg agtatgttat taaacctaaa aaacaaatga
     4501 aaggtggtaa gtaaattgag tttaacaacg atttttgcat tgctattttc atcaatgcta
     4561 atttacgcaa cacctcttat ctttacaagt attgggggaa ccttctctga acgtggtggt
     4621 atcgtcaacg ttggtttaga aggaattatg gtaattggag ctttctcagg cgttgtattt
     4681 aatttagagt ttgcttctgt ttttggtgat gcgactccat ggatttctgt actagttggt
     4741 ggtttagttg gtttaatttt ttctgttatc catgcagttg cgacagttaa ttttcgtgca
     4801 gatcatatta tcagcggaac cgttcttaat ttaatggcac cttcattagc tgttttcttg
     4861 attaaggttc tttataataa aggtcaaact gataatattc aagaatcatt tggaaaattt
     4921 aatttcccta tactatcaga tattcctttt gttggtgata tcttctttaa aggaacaagc
     4981 ttagtcggtt atattgctat tcttttctca ttccttgctt ggtttattct atataaaact
     5041 cgctttggat taagattacg ctcagttgga gaacatcctc aagcagcaga caccttaggt
     5101 attaatgttt atcttatgcg ttactctggt gtcttgattt caggtttcct tggtggaata
     5161 ggaggagctg tatatgctca atctatttct gttaactttg cagcgacaac tatattagga
     5221 ccgggcttta tctccttagc ggcgatgatt tttggtaagt ggaatccaat tggtgcaatg
     5281 cttgctagtc tattctttgg tttatcgcaa agtttagcag ttatcggtag ccatttacca
     5341 cttttgagca atattccaac agtttatcta caaattgctc catatgtcct cactatcatt
     5401 gtattagcag ctttctttgg acaagcagta gctcctaagg cggatggtat taattatatt
     5461 aaaacgaaat aaataaaaag aaactagtat aagctagttt ctttttattg tttgtgaatt
     5521 tgtgaaaaaa tactttgtga aaatttcaca taaattataa tacaataaca ataaaatata
     5581 aaaaaataga aaattgagtc attattctag taagagtttg tgaataatgt tataatgtgt
     5641 tttgttatta attatttata aaggagaact cttaatatga gtaaaatcgt tgtcgtaggg
     5701 acaaaccacg ctggtacagc agcaattaaa acaatgctta gtaattatgg tgaagccaat
     5761 gaaattgtaa catttgatca aaattccaat atctctttct taggatgtgg tatggctctt
     5821 tggattggtg aacagattga tggtccagaa ggattgttct actctgataa agagcagttg
     5881 gaaagtatgg gagctaaggt ttacatgaac tctccagtac ttaatattga ctacgataaa
     5941 aaagaagtta cagctttagt agatggaaaa gaacatgttg aaagttatga aaaacttatt
     6001 ttagcaactg gttcacaacc tatcattcca ccaattaaag gtgttgaaat tcaagaagga
     6061 tctcgtgaat ttaaagctac tcttgaaaac cttcaatttg tgaaacttta tcaaaattca
     6121 gaagaagtta ttgaaaagtt agctaaacct ggtatcaatc gtgttgctgt tgttggggct
     6181 gggtatattg gtgtagaatt agcagaagct ttccaacgta ttggaaaaga agtaacactt
     6241 gttgatgttg ctgatacttg tatgggtggt tactatgatc gtgactttac tgatatgatg
     6301 agtaaaaacc ttgaagacca tggtattcgt cttgctttcg gacaagctgt tcaagcagtt
     6361 gaaggtgatg gtaaagtaga acgtcttgtt actgataaag aaacatttga tgttgatatg
     6421 gtgatcttag ctgttggatt ccgtccaaat acagaactcg gtgcaggtaa attagataca
     6481 ttccgtaatg gcgcttgggt agttgataag aagcaagaaa caagcgtgaa agatgtttat
     6541 gctataggtg attgtgcgac aatttgggat aattctcgtg atgatatcaa ttatattgct
     6601 cttgcttcaa acgccgttcg tactggtatt gtagcagctc acaatgcatg tggtacagag
     6661 cttgaaggtg ctggtgtcca agggtcaaat ggtatctcaa tctatggcct taacatggtg
     6721 tcaactggtt tgactttaga aaaagcaaaa caagctgggt acaatgcagt tgaaacaggc
     6781 tttaatgacc ttcaaaaacc agaatttatc aaacacaata atcatgaagt agctattaag
     6841 attgtctatg ataaagatag ccgagttatc cttggttgcc aaatggtctc acacgaagat
     6901 gtatctatgg gaattcatat gttctcactt gctattcaag aaaaagttac tattgaaaag
     6961 cttgctttga ccgatatctt cttcttacca cattttaata agccatataa ctatattaca
     7021 atggctgcat taggcgctaa agattaattt aaaattaaaa aggttaagcg aaatgcttag
     7081 ccttttattt catcctttaa caaggtcaaa aatgtaacgg caaagaaaaa gctaagatat
     7141 tcctatccta gctttttttc aactatgtta gttttttgat gcttcttgga attctgggtt
     7201 tttccaagct tcgtcaataa tatcttttaa ttgttcagct gaggcttgca ttttttgaag
     7261 ttcagcatca tttaatggga tattaactgg acgaacgata ccatgtgcac ctacgattgc
     7321 aggctgaccg ataaagacat ctttaacatc accgtattgg ccttcttgat atacagataa
     7381 tggaagaact gcattttcat catcaaggat agctttagta atacgagcaa gtgcgacagc
     7441 aataccgtaa tatgtagcac cttttttgtt gatgattgag tatgcagcgt cacgaactga
     7501 aataaacaaa tcaacaagtc cttgttcatc aatatcgcga ttttcttgta accattgttc
     7561 aagttggaca ccagcaacgt tagcatgtga ccaaacggca aattctgagt caccgtgttc
     7621 acccatgatg tatgcgtgaa ctgaacgagc atcaacacca attttatctg ctaaagcttg
     7681 acggaaacgt gcagagtcaa gtgaagtacc tgaaccaata acgcgttcct tagggaaacc
     7741 tgagaatttc caagttgaat atgtaagtac atctacaggg ttagccgcta ctaagaagat
     7801 accgttgaaa cctgattcaa caacttgagt tacgattgat ttgttaattg ctaagttttt
     7861 accaacaagg tcaagtcgag tttcacctgg tttttgaggt gcgccagcag taatgacaac
     7921 aaggtcagca tctgcgcagt ctgcataagt agctgcgtag atttttttag gtgatgtaaa
     7981 tgcaagggca tgtgaaagat cttcagcatc accaacagct ttatcaaata aagctgggat
     8041 ttcaatgata cctaactctt gcgcaatacc ttggttaaca agtgcaaaag cgtaagaaga
     8101 acctacggca ccatcaccaa cgagaataac ttttttatgt tgtttagttg cagtcatttc
     8161 taaacatctc cttaaatatt tttgggaagt ttcccataca cctatattct atcattttca
     8221 atgaaagaag tcactaataa aatgtgaaaa aatttgcaaa caaattgaaa tatcatagaa
     8281 aactattgaa aacgctgagt gtcttgatat ttttgatacc gatttatcat cgcaatttta
     8341 cctaaattat ggtacaatgt aagagactta cagtgaagaa aaggggcatt ttttaatgca
     8401 agataaaaat ttagtagatg ttaatctaac tagtgaaatg aaaacgagtt ttatcgatta
     8461 cgccatgagt gtcattgttg ctcgtgcact tccagatgtt agagatggtt taaaacctgt
     8521 tcatcgtcgt attttatatg gtatgaatga actaggtgtg acacctgata aacctcataa
     8581 gaaatcagca cgtattactg gtgatgttat gggtaaatac catccacatg gtgattcatc
     8641 tatttacgaa gcaatggtgc gtatggcaca atggtggtca tatcgtcata tgctcgttga
     8701 tggtcatgga aactttggtt caatggatgg ggatggtgct gccgcacagc gttatacaga
     8761 agcacgtatg agtaaaattg ctcttgagat gcttcgtgat atcaataaaa atacagttga
     8821 ttttcaagat aactatgatg gcagtgaacg tgaacctctt gttttaccag cgcgtttccc
     8881 taatttatta gtcaatggtg caactggtat tgctgtaggt atggcaacaa atattccacc
     8941 acataatcta ggtgagtcta tagatgctgt caaattggtt atggataacc ctgatgttac
     9001 tacacgggag ttaatggaag ttattcctgg tccagatttt cctactggtg ccttggtgat
     9061 gggacgttca ggtattcacc gtgcctatga aactgggaag ggatcaattg tcttacgttc
     9121 acggactgaa attgaaacaa cttcaaatgg gaaagagcgt attgttgtca cagagtttcc
     9181 atatggcgtt aataaaacaa aagtacacga gcatattgta cgtttagcac aagaaaagcg
     9241 cattgaaggt attactgctg ttcgtgatga atcaagtcgt gaaggcgttc gttttgttat
     9301 tgaagttcgt cgtgatgctt cagcaaatgt gattttaaat aatcttttca aattaactag
     9361 cttgcagaca aattttagct tcaatatgct tgctattgaa aaaggtgttc caaaaatttt
     9421 gtcattacga caaatcattg ataactatat tgaacaccaa aaggaagtta ttgttcgtcg
     9481 tactcagttt gacaaagcta aagcagaagc ccgtgctcat atcttagaag gtttacttgt
     9541 ggcgcttgat cacttggatg aagttatcac aattattcgt aatagtgaga cagacaccat
     9601 tgctcaagca gagttgatgt cacgttttga attatcggag cgtcagagtc aagctatttt
     9661 agatatgcgt cttcgtcgtt taactggatt agaacgcgat aaaatccaat cagaatacaa
     9721 tgacttacta gctttaattg ctgatttagc agatatctta gctaagcctg aacgtgttgt
     9781 taccattata aaagaagaga tggatgaggt taagcgtaag tatgctgatg cccgtcgtac
     9841 agaattaatg attggtgaag ttttatcact tgaagatgaa gaccttattg aggaagaaga
     9901 tgttcttatt actctctcaa acaagggata tatcaagcgt ctcgctcaag atgagtttag
     9961 agcacaaaaa cgtggaggac gcggtattca agggactgga gttaataacg atgattttgt
    10021 tcgtgaattg gtttcaacaa gtactcacga tactgtgctt ttctttacaa atttagggcg
    10081 tgtgtatcgt ttgaaagcat atgaaattcc agaatatgga cgtacagcta aaggcctccc
    10141 tatcgttaac cttttaaaac ttgacgaagg tgagacaatt caaacaatta ttaacgctag
    10201 aaaagaagat gtcgctaata aatatttctt tttcacaact cagcaaggta tagtgaaacg
    10261 tacaagtgtt tctgaattta gtaacattcg tcaaaatgga cttcgtgcta ttaatttgaa
    10321 agagaatgac gagttgatta acgtcctctt gattgacgaa aatgaagatg ttattatcgg
    10381 gacacgtaca ggctactctg ttcgcttcaa agtgaacgct gtacgtaata tgggacgcac
    10441 agcgactggg gtacgaggcg ttaatcttcg tgaaggtgat aaagttgtgg gagcttctcg
    10501 tatcgttaat ggacaagaag tactaatcat cactgaaaaa ggttatggca agagaacaga
    10561 agcttcggaa tatcctacaa aaggccgtgg tggtaaagga ataaaaaccg ctaatattac
    10621 tgctaaaaat gggccattag cacgcttagt gacgattaat ggaaatgagg acatcatggt
    10681 cattacagat acaggtgtta ttattcgaac caatgttgct aatatttctc aaactggtcg
    10741 ttctaccatg ggtgttaaag tgatgagact tgatcaagaa gctaaaatcg tcactgttgc
    10801 gctagtggaa caagaaattg aagataagtc taatatagag gatacaaaag aataggaagt
    10861 tatgcgtaat aaaaaaaaat cacatggttt ctttaacttt gttagatggt tattggttgt
    10921 attgctaatt attgttgggt tggctttggt gttcaataaa ccaattcgca atgctttcat
    10981 agctcatcaa tcaaatcatt atcaaatttc acgagttagt aaaaaaacaa ttgaaaaaaa
    11041 taagaaatct aaaacaagtt atgacttttc atcagtcaaa tctattagta cagaatcgat
    11101 tttatctgct caaacgaaat cacataattt gcctgtaata ggagggattg ctataccaga
    11161 tgtcgaaatc aatctcccaa ttttcaaagg actgggcaat acagagttga gttatggtgc
    11221 aggaacgatg aaagaaaacc agattatggg aggcccaaat aactatgcct tagctagtca
    11281 ccatgtcttt ggtttgacag gttcttctaa gatgctcttt tcacctttag aacatgctaa
    11341 gaaaggtatg aaagtttacc tcactgataa aagcaaagta tacacttaca ctattactga
    11401 aatttcaaag gtaacaccag aacatgttga ggttattgat gatacacctg gcaagagcca
    11461 actcacttta gtgacctgta cggatccaga agccacagaa cgtattattg tgcatgctga
    11521 attagaaaaa acaggtgaat tttcaacagc agatgagtcg atattaaaag cattttcgaa
    11581 aaaatataat caaattaatc tctagaaggt agttagaatt atgaaattaa aggctgttca
    11641 tcatattgcg ataattgttt ctgactatga aaagtcaaaa gacttctatg ttaataagct
    11701 aggatttgaa attattcgtg aaaaccatcg tccggagcgt catgattata aattagattt
    11761 acggtgcggt gatattgagc ttgaaatatt tggaaatcgt cttgatgatc cagaatatga
    11821 aactcctcct cagagaatag gtaggccgaa ttggccacgt gaagcatgtg gtttacgaca
    11881 ccttgccttt tacgttccag atgtggaagc gtacaaggta gaactggaga atttaggaat
    11941 ttttgtagag cctattcgtt acgatgacta tactggcaaa aagatgacct ttttctttga
    12001 tccagatggt ttacctttag aattacatga ataataaaca aagttgcgtg gatacgcaac
    12061 tttgtttaat ttactgaata attttatatt caagaagttt tttaaagaaa taagtaagtt
    12121 aatcatggta taatgtaagg tatgaaaaga attgaaaagg aattattttg aaatgaggat
    12181 actaaaatga gtattaaaaa gctattgttt gcaggccttg cttttatttt atttacccta
    12241 gcgagtccag catatgctgc atcagatgtt cagaaagtta ttgatgaaac ttatgttcaa
    12301 cctgattatg tcttaggtta ttcgctaaat caagaacagc gtgcacagac attacaactt
    12361 ctgaattatg atgaatctag agacacgaaa gttaaaacgc taaatactag ctcatatgct
    12421 aaaataatga atatagctga tgatgcaagc atccaattat attcatctgt taaaataaaa
    12481 aaattagggt ctaacgatac cttagcagta aacattgtta cacctgagaa tattactaaa
    12541 gttacagaag atatgtatcg caatgctgca gttacattag gtattgagca tgcaacgata
    12601 tcagtggctg ctccaataaa agtaactggg gagagtgctc ttgcaggtat ctattattca
    12661 ttagagaaaa atggagcaag tgtatcatca gaaaataaac aactagcaca agaagagtta
    12721 agtactttat ctggtattaa tgctgagaat aagggtaagg aaggttatga cgccgacaag
    12781 cttaatgtag ctttaacgga tattaagtca gccgtagcta aaggaggatc tgatctttca
    12841 aaagatgata ttcgaaaaat tgttgaggaa acattgaaaa attatcatct tgataacgct
    12901 gtaacggaaa atcaaataaa tttaattgtt aattttgcag ttaatctttc acaaagtaat
    12961 gtcatcaaaa atagtgattt cacgaacacc ttgaataatt tgaa
//
LOCUS       DATROY010000048        12970 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.049, whole genome shotgun sequence.
ACCESSION   DATROY010000048 DATROY010000000
VERSION     DATROY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12970
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..79
                     /locus_tag="VBD11_001072"
     CDS             <1..79
                     /locus_tag="VBD11_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO6954032.1"
                     /translation="ALTVDKKILLKQGGLPILALLTFLF"
     gene            213..956
                     /locus_tag="VBD11_001073"
     CDS             213..956
                     /locus_tag="VBD11_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6954033.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            940..3480
                     /gene="mprF"
                     /locus_tag="VBD11_001074"
     CDS             940..3480
                     /gene="mprF"
                     /locus_tag="VBD11_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO6954034.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(3559..5073)
                     /locus_tag="VBD11_001075"
     CDS             complement(3559..5073)
                     /locus_tag="VBD11_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6954035.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(5076..5798)
                     /locus_tag="VBD11_001076"
     CDS             complement(5076..5798)
                     /locus_tag="VBD11_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954036.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            complement(6020..6853)
                     /locus_tag="VBD11_001077"
     CDS             complement(6020..6853)
                     /locus_tag="VBD11_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6954037.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            complement(6850..8145)
                     /locus_tag="VBD11_001078"
     CDS             complement(6850..8145)
                     /locus_tag="VBD11_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6954038.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            8248..9246
                     /gene="argF"
                     /locus_tag="VBD11_001079"
     CDS             8248..9246
                     /gene="argF"
                     /locus_tag="VBD11_001079"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6954039.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            9258..10184
                     /gene="arcC"
                     /locus_tag="VBD11_001080"
     CDS             9258..10184
                     /gene="arcC"
                     /locus_tag="VBD11_001080"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6954040.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            10278..11831
                     /locus_tag="VBD11_001081"
     CDS             10278..11831
                     /locus_tag="VBD11_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6954041.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(11941..>12970)
                     /locus_tag="VBD11_001082"
     CDS             complement(11941..>12970)
                     /locus_tag="VBD11_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6954042.1"
                     /translation="SLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSL
                     GIVLPLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTEL
                     AAVINTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFE
                     VIHRQSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLD
                     SQIEDDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGIS
                     VDDQSKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTL
                     TFPKLKKD"
BASE COUNT         4037 a         1979 c         2469 g         4485 t
ORIGIN      
        1 tgctttgaca gttgataaaa aaatcttatt aaaacagggt ggtttaccta tattagctct
       61 tttaacattc ttattttaat actacttagc cgttcgattt agttgaacgg cttttagtaa
      121 tcattttttt ctcataatac aggtagttta agtaatttgt ctttaaaaat agtataatat
      181 aactatgaat tcaaagagag gtgactttgg ttatgactga gaactggtta catactaaag
      241 atggttcaga tatttattat cgtgtcgttg gtcaaggtca accgattgtt tttttacatg
      301 gcaatagctt aagtagtcgc tattttgata agcaaatagc atatttttct aagtattacc
      361 aagttattgt tatggatagt agagggcatg gcaaaagtca tgcaaagcta aataccatta
      421 gtttcaggca aatagcagtt gacttaaagg atatcttagt tcatttagag attgataaag
      481 ttatattggt aggccatagc gatggtgcta atttagcttt agtttttcaa acgatgtttc
      541 cagatatggt tagagggctt ttgcttaatt cagggaacct gactattcat ggtcagcgat
      601 ggtgggatat tcttttagta aggattgcct ataaattcct tcactattta gggaaactct
      661 ttccgtatat gaggcaaaaa gctcaagtta tttcgcttat gttggaggat ttgaagatta
      721 gtccagctga tttacagcat gtgtcaactc ctgtaatggt tttggttgga aataaggaca
      781 taattaagtt aaatcattct aagaaacttg cttcttattt tccaaggggg gagttttatt
      841 ctttagttgg ctttgggcat cacattatta agcaagattc ccatgttttt aatattattg
      901 caaaaaagtt tatcaacgat acgttgaaag gagaaattgt tgaaaaagct aattgaaaaa
      961 gtcaaatcac tgacttctgt gattaaaatt gtatttttta tatctgtttt agtgcttatt
     1021 attgttgaaa tgattcattt gaaacgaact atttctgttg agcaactaaa gagtgttttt
     1081 gggcaattat ctccaatgaa tcttttctta attatccttg tgggggttat cgctgtctta
     1141 ccgacaaccg gatatgactt tgtactgaat ggacttttac gtacagataa aagcaaaagg
     1201 tatattttac agactagttg gtgtatcaac acttttaata acttgtcagg attcggtggc
     1261 ttaatcgata ttgggttgcg catggctttt tatggtaaaa aaggtcaaga gaagagtgac
     1321 ctaagagaag tgactcgttt tttaccctat cttatttctg gtctgtcatt tattagtgtg
     1381 attgccttaa tcatgagcca tatttttcat gccaaagcta gtgttgatta ctattatttg
     1441 gtattaattg gtgctagtat gtattttcct gttatttatt ggatttctgg tcataaagga
     1501 agccattatt tcggagatat gccatctagt actcgtataa aattaggtat tgtttctttt
     1561 tttgaatggg gatgtgcagc cgcagcgttt ataattatcg gttatttaat gggcattcat
     1621 ctaccagttt ataaaatttt accactattt tgtattggtt gtgccgtcgg gattgtatcc
     1681 cttattcccg gtggattagg aagttttgaa ttagttctat ttacagggtt tgctgccgag
     1741 ggactaccta aagaaactgt ggttgcatgg ttattacttt atcgtttagc ctactatatt
     1801 attccattct ttgcaggtat ctatttcttt atccattatt taggtagtca aataaatcaa
     1861 cgttatgaaa atgtcccgaa agagttagta tcaactgttc tacaaaccat ggtgagccat
     1921 ttgatgcgta ttttaggtgc attcttaata ttttcaacag cattttttga aaatattact
     1981 tatattatgt ggttgcagaa gctaggcttg gacccattac aagaacaaat gttatggcag
     2041 tttccaggtt tattgctggg ggtttgtttt attctcttag ctagaactat tgatcaaaaa
     2101 gtgaaaaatg cttttccaat tgctattatc tggattactt tgacattgtt ttatcttaat
     2161 ttaggtcata ttagttggcg actatctttc tggtttattt tactattgtt aggcttatta
     2221 gtcattaagc caactctcta taaaaaacaa tttatttata gctgggaaga gcgtattaag
     2281 gatggaatca ttatcgttag tttaatggga gttctatttt atattgcagg actactattc
     2341 cctatcaggg ctcatattac aggtggtagt attgaacgcc tgcattatat catagcatgg
     2401 gagccgatag cattggctac gttgattctt actctcgttt atttatgttt ggttaagatt
     2461 ttacaaggaa aatcttgtca gattggtgat gtgttcaatg tggatcgtta taaaaaacta
     2521 cttcaagctt acggtggttc ttcggatagc ggtttagcct ttttaaatga taaaaggctc
     2581 tactggtacc aaaaaaatgg agaagattgc gttgcgttcc aatttgtaat tgtcaataat
     2641 aaatgtctta ttatggggga accagccggt gatgacactt atattcgtga agctattgaa
     2701 tcgtttattg atgatgctga taagctagac tatgaccttg ttttttacag tattggacag
     2761 aagttgacac tacttttaca tgagtatggt tttgacttta tgaaagttgg tgaggatgct
     2821 ttagttaatt tagaaacgtt tactcttaaa gggaataagt acaaaccttt cagaaatgcc
     2881 ctaaatagag ttgaaaagga tggtttctat ttcgaagttg tacaatcgcc acatagtcaa
     2941 gagctactaa atagtttgga agagatttct aatacttggt tagaaggacg tcctgaaaaa
     3001 ggtttctcac taggatattt taataaagat tatttccaac aagccccaat agctttggta
     3061 aaaaatgctg aacacgaagt tgttgctttt gctaatatta tgccaaacta tgaaaagagt
     3121 attatctcta ttgatttaat gcgtcacgat aaacagaaaa ttccgaatgg cgttatggat
     3181 ttcctctttt tatcattatt ctcttattat caagagaagg gataccacta ttttgatttg
     3241 gggatggcac ctttatcagg agttggtcgc gttgaaacaa gttttgctaa agagagaatg
     3301 gcgtatcttg tctatcattt cggtagtcat ttctactcat ttaatggttt acacaagtat
     3361 aagaagaagt ttacaccatt gtggtcggaa cgttatattt cttgttctcg ttcgtcctgg
     3421 ttaatttgtg ctatttgtgc cctattaatg gaagatagta aaattaagat tgttaaataa
     3481 gctttatttg gcaattaaaa agagcatgtc atgcgacatg ctctttttaa atcatttaat
     3541 accattgatt gcttgaatct actttataat atgatgtgct tttaaatatt gtttagctac
     3601 tgtagctgct gatttatgct ttacagctac ttggtagttc atttcttgca tttctttttc
     3661 agtgatatga ccagcaagtt tattgagagc tttttttact tgagggtatt ttttaatcgt
     3721 atcttgcctt agtagcggtg ctccttgata tggcggaaat agatgcttat cgtctttgag
     3781 aatttgaagt tgatactgga ttaattcact atcggtagag taagcgtcaa taatattgac
     3841 atctttacta ttgatagctt ggtatcgtaa agcaggttct aaggttgaga tatcaaggtt
     3901 taaaccataa tgtttctgta aaccaatact cccatcctct ctatccttaa attctaaagt
     3961 gaaaccagct tttagtttat ctaatttttt taggtcgctg atatttttga gttgattagc
     4021 ttcagcgtaa tcttttttaa cagctacagc gtaggtgttt tgataagcca taggtgacaa
     4081 aagtgacaac ttatcttgtt ttaaaatacc gtttttggct aagttgtaaa cctgtttagg
     4141 gttattactt actttaggag gatttttaag taaacttgat gtaatagtac ctgtaaactc
     4201 tgggtaaaga tcaatatcac cagattttag ggcttgatat aagaaagttg tttttccgaa
     4261 attaggttta agcttaactt tgatatctgt ctgatcctca ataagttctt tgtacatatt
     4321 aattaaaata tctggttcag ttcctaactt ccctgcaaca gttatcgttg cagtatttgt
     4381 tccaggcatc cattttggag cataggatag tcctaagcct gctagtaaaa gtataccaga
     4441 aacggctatg gttcgtaatc tagccttttc tagtaatcca attaaaccac taaatataat
     4501 agctaagaca gcagaagaga ttgcccctat gagaattaga gaaggattgt tgcgatcaat
     4561 ccctaacaaa ataaatgatc caaggccacc agctccgata agactagcta atgtagctgt
     4621 accaataatc ataacagagg cagttctaat gcctgagatg ataacaggca tacttaaagc
     4681 taactcaaac tttttaagcc tttcccaacg tgtcattcca aaagcggttg ctgcctcaat
     4741 gagattagca tcaatttgca tcagtgctgt tacagtattt tgtaaaattg gaaatagagc
     4801 gtaaataatt agtgcaacga cagctggtac agttccaatt cccataaaag gtataaataa
     4861 accaagcaag gctaaagaag gaattgtttg aaatatccca gttatctgaa ggacaacatg
     4921 actgattttt ttataatgac taatgatgat accgagtgga atagcaatta aagttgctaa
     4981 aataagtgtt agtagtgata gctggaggtg ttcaataaga ctttgagtcc aatctccaaa
     5041 acgctcttga aaggtagtga ttagtgttgt catactcagt tctcctcttc aaatagggtt
     5101 ctaacaaatt ggtcagaggg attattttga attatttctg gtctatccaa ttgcactatc
     5161 ttaccttcct taataacaca aattctatct gcaagacgca tagcttcctt catgtcatgg
     5221 gtgacaaaca ctaatgtaat acccaattca gactgtaatt gtttggtgat atcttgtaat
     5281 tgacgtcttg agatagggtc taaagcagaa aatggctcat ccataagtag taccttaggt
     5341 ttagcaacga ttgctcgtag gatacctatg cgttgttgtt ccccaccaga gagctcatga
     5401 ggatagcgat taaagtagtc cttagcaggt aaacccacct tatcaagaag gtcgctagcg
     5461 gctttttttt ggtcaccttt tgaccagccc ttcatttcag ggataagttc tatattttct
     5521 ccaactgtta aattagggaa aagtgcaatt tgttgcaaga cataaccagt ttctaagcgt
     5581 aattgtcgca aatcaagtga tgtaatgttt tccccatcta aagtaatagc gccttggctt
     5641 ggttcgatga gacgattaat catcttgagg atggtagttt taccactgcc actggccccc
     5701 acaaggacaa agaattctcc tctttgaata tggcaactaa tattatcaat gatggtatga
     5761 tcaccatatg atttactaac attttcaaat gaaatcatgt aatttacttt ctagtgcgta
     5821 acgagtgtag gtcgtatgat attagggtta tattagcata aagtagacat tcaaacaact
     5881 atctgacatt taaattgtca gaaacgataa actagaagca aaaacaaaca ccttgcggta
     5941 gacaaggtgt ttgttggaga tttatgaaaa gaaaagtttt aggatggtta tagtatataa
     6001 ggccatgctt aaacaagtct taaaacgttt tactctgtaa aataagttca tcgaaaaaac
     6061 gtttaagtga aattttacca ccatacatgc tttttccttg aataagttgt gcctcattat
     6121 ggatattctg aaatccaaac aatgcattag catattgcag tgttaattca ttttcaaaat
     6181 catcaatgta cagatgagcc atatttatca tagctttttt aacggcacga cgaatacgtt
     6241 gtaaaatgat tttttgttgg tgttcatcga tagaaagttc tttttgaaaa tcaaattggg
     6301 caaaatttaa ttcttgtttt aataaaagtt cacagatgtt aagaatatct gtgtaggctg
     6361 tctctgaagt aatacctaaa taactgagta ttgaacggat tttagtgagg ttactagtcg
     6421 ttataggctt ttgatatgat ggcgtatttt ctaggagatt ttggattgta tttaattttt
     6481 tttgcatttc gatagtatca gtgacacgtt tcacgacgct ttttacttca ataatattaa
     6541 tgggtttatt aataaaaaat tcgattcctg ctttatatgc ttcttgtcgt aaatcattat
     6601 ctttgacttg ggaaatcata ataaatttta aatcggaacg ctgtttatag attttttgga
     6661 ctaatgttac accgtctaat attggcatta ataaatcaat caatacgata tcaacatctg
     6721 caatcagcaa ttcgttgtat gctttactgg agacattatt cactctgact actgtattat
     6781 tgaaatcttc ttcaataata tcttgtaaaa tcatagtgat agtggggtca tcatcaataa
     6841 tatagaattt cacgtattat ctcctaactt aaatcgattt ttacggaata acagtgtaaa
     6901 tgttgttcca tttggctgat taggacaaac agttatcgtt ccttgatatt gctcttccat
     6961 taaaatacgc acgtgggata gcccgattcc tcgatagata tcaccattag catcaaattt
     7021 agtcgaaaat ccaggtttga aaatcatgtg tttcatttta tcaggtattc ctggtccgtt
     7081 atcactaatc tcaattttaa taaaatcttc taattcttct gtgtgaacac tgattgtacc
     7141 ctttttttgt ttatcaatag cttctactgc atttaaaacg atattggaaa taatggttag
     7201 taaatagtaa tagtttgata ttatcaaatt acatttatta ttttcgagaa taatgatatc
     7261 ttgatgccga aattgaataa tactcctagt ataagataaa acaatttgaa agatatcttt
     7321 caatgccata gttgattcat tttttacact aaaataggtt cctaagcctt taataatatt
     7381 ttggtaatct ttcttaactt cgtgaacgtc ccgcgaaata tctagagaga ggtgttggta
     7441 ttctttaggt aggtgatatt tggataactc tttatctaat aaataagcat ttttcatgat
     7501 gttttcaatt tcaatgatat ttttttgcat aaaataaacc tcgcttttaa cggctgaagt
     7561 tgaccacata aaataatagt atcgacgttc gtgatcgtct ttttgtaaga gaagtgatag
     7621 ataactataa gtataagcca cagcgcaact aataaaagct cgcaaaagtg ctatggcaaa
     7681 taaagtagcg aatatagtta tagtataatt gttaaacttt atgagaaaac taatttctac
     7741 aaggttggca aaataatcac aaataatgat agaaaagaaa aaagtacctt tattacggta
     7801 ggaacgatgc caataaatag tataaaaggt gatgccataa caaatataaa acgccatatc
     7861 agttaaaacg aattcaatta tttgatgaat ctcagcctcg ccagcgatac ttaatatgat
     7921 tcctctgaag ataggagaag caaaagtaat tcctagacag agaagaattg gattaatgtc
     7981 gtcattaaaa tataaaaaga caggcaaaat gaacaaagat aaggtcataa taaacccatc
     8041 agcaagtatt gagatattga tttgagcagc taaggcgata gctattgcag agataataat
     8101 acgctgcctt ctttctagat gtttttctga taatatatct agcatataaa tgtccttaaa
     8161 gtttttttga agatttttga tgaattttga ttatttttga aagcgctatc tacaataaga
     8221 gtataaaaaa aaaggaggca ataagcaatg aaaaatttac gtaaccgtag ttttcttact
     8281 ttattggatt ttagtacagc agaagttgaa tttcttttaa aactttcaga agatttaaaa
     8341 cgtgctaaat acgcaggaat tgaacaacaa aaacttgttg gtaaaaatat cgctttgatt
     8401 tttgaaaaag attctacacg gacacgttgc gcttttgaag ttgctgctca cgatcaagga
     8461 gctcatgtta cttatttagg tccaacaggc agtcagatgg gtaaaaaaga gacctcaaaa
     8521 gacactgcgc gtgtgctagg tggtatgtat gatggtattg agtatcgtgg attttctcaa
     8581 gaaacagttg agactttagc cgaattttca ggagttcctg tgtggaatgg tttaaccgat
     8641 gctgatcatc caacgcaagt actagccgat tttctgaccg ctaaggaatg cttacataag
     8701 ccctataaag atattcgctt tacgtatgtg ggtgatggac gtaacaatgt cgctaatgcc
     8761 ttgatgatcg gtgcttctat tgtaggcatg acttatcatt tggtttgtcc taaagaatta
     8821 gagccagatc ctgaactact ctctaaatgt caagaaattg cgaaaactac tggtgcttct
     8881 attgaaatca ctgctgatat tgcagaaggc gtcagagatt cagacgtcct atatacagat
     8941 gtttgggttt caatgggaga accggatgaa gtttggaaag aaagaatagc tcttttagaa
     9001 ccctatcgta ttacccaaga aatgcttaat atgacagaaa atccaaacgt tattttcgag
     9061 cattgcttgc cctcatttca taatatagat actaaagttg gatacgacat ttatgaaaaa
     9121 tatggtttga aggaaatgga agtatcagat gaagtttttg aaggccctca ctcagttgtt
     9181 ttccaagaag ctgaaaatcg tatgcatact attaaagcag ttatggtagc aactttaggt
     9241 gattaggagg aattttcatg gcaaaaattg ttgttgcact aggaggaaat gccttaggaa
     9301 attctccaga agaacaatta agattagtaa aacatactgc aaaatcattg gtggccttga
     9361 taaaaaaggg acacgaaatt gttgtaagtc atggtaatgg accacaagtt ggtgccatta
     9421 atttaggaat gaattttgcg gctgagagtg gtcaaggaac taattttcct tttccagaat
     9481 gtggagcaat gagccaagga tacataggct atcatctcca acagagcctt ttaaatgaat
     9541 tacgtcaaga gggaataaat aaggaagttg ctacaattat tacccaaatt gaggtggatg
     9601 aatcagacca agcatttagc gcacctacca agcctatagg caccttttat gataaggaga
     9661 catcagaaaa gattgctata gagaaagggt atactttcgt agaagacgct ggacgagggt
     9721 atcgtcgtgt tgttgcttca cctgaaccta aaaaaatcat cgaaattaat agcattaaaa
     9781 cacttattga aaatgatacc ctagttatcg caggtggagg tggaggcatc cctgtcatta
     9841 ataagggagg ttatgaaggt attgcagctg ttattgataa agacaaatct agtgcactgt
     9901 tagcaggaga actagctgcg gatcaattga ttattctcac tgcagttgat tatgtttata
     9961 ctcaatttgg taaagaaaat caaaaagctt taacagaggt taatgagaat caaatgattg
    10021 attacgttaa ccaaggtgag tttgcaaaag gaagtatgtt acctaaagta atagcctgta
    10081 tgagtttctt agatcacaat ccgaaaggaa tagccttaat cacctcatta aatggtttag
    10141 aagatgcatt agatgggaaa ttaggaacac gtataactaa gtaatctcag ataagtgcaa
    10201 agaaatgatt atttaacctt catctttgca ctttttctga tgaagtcaat ctatttttat
    10261 tatagaggag gcgtgtcatg ccagaaaaaa tgacaaacag tgaaaacaaa aggagtaaag
    10321 gttttcgaat gccaggtgcc tttacaatat tattcatttt aacaatcttt tcggttttgg
    10381 ctacatggtg gatacctgct gggtcctatt caaaattaca atttgataca gcatcatcca
    10441 aactagtagt aacagatcca aatggtaaga cagttcatgt tccagcaacg caaacacagt
    10501 tggataagat gaatgtcaaa attaaaatta aagaatttac ttctggtgct attagtaagc
    10561 cagtttcagt tccaaatact tacaaacgtt tgaagcaaaa tccagcaggc attggctccg
    10621 tgacgactag tatggtaaat ggaaccattg aagctgttga tattatggta tttatcatgg
    10681 tacttggagg catgatagga gtagttagaa agagtggcgc ttttgagtct ggtcttctag
    10741 ctttaacaaa gaaaaccaaa ggtagagagt ttttattgat atttttagtt tccttattga
    10801 tggttttagg cggtactcta tgtggtattg aagaagaagc agttgctttt tatcctattt
    10861 tagttccaat ctttttagcc atgggttatg attctatcat ttgtgtaggt gctattttct
    10921 tagcaagttc tgttgggaca tcattttcaa caattaatcc gttttcttct gttattgctt
    10981 caaatgcagc tggtattagt ttcacagaag gcttatcatg gcgtacagca gggtgtattg
    11041 caggtgctat ttttgttgtg gtgtatctac attggtatgc taaaaaaata aaagcgaatc
    11101 cagagttttc atattcctat gaagatagag tagaatttaa tgctaaatgg ggcatgacaa
    11161 ctaaccatac accctcactc tttactatta gacaaaaaat tattttaagt ttatttgtta
    11221 tttcatttcc tttgatggtt tggggagtaa tgtcacaggg atggtggttt ccaaccatgg
    11281 cttcttcctt tttagctatt acaattatta ttatgttttt aacagcaact ggggcaaatg
    11341 gtattggaga aagggatgtt gttgatgagt ttgttaatgg tgcatcaagc cttgttgggg
    11401 tttcattaat cattggttta gcgcgtggta tcaatattat tttgagccaa ggttatatat
    11461 cagatacgat gctttacaca gcttcaaaat tagcttcaca tgttagtggc cctgtcttta
    11521 tcattgttat gatgtttatc tactttgtgt taggatttgt tgttccatcc tcgtcgggtt
    11581 tagcagtact ttcaatgcca attctagcac ctcttgcaga tactgttggg attcctcgtt
    11641 cagtagtagt aatggcttat caatttgggc agtatgcaat gttgttttta gcacctactg
    11701 gactagttat ggcaaccttg cagatgttag atatgaagta ctcccattgg ctaaaatttg
    11761 tgtggccggt tgttttattc ctcctaatat ttggtggcgg tttgttagtt ctacaagttt
    11821 taatacttta aagatttcag ctattagtct caaaaatatc tgaagagata taataaattg
    11881 agtttaagtt aaataaaaaa aacgataggt gtaatctatc gtttttttta tttcctttct
    11941 ctagtctttc ttcaattttg gaaaggtgag cgtaaactga ctgccatcat caggtttaga
    12001 atcaacccat attttagcac ggtgtattgt agcgatttgt tttactaaag atagccctaa
    12061 accaattcca gactgactat ccttggttcg agcagagtcc acttgataga aacggttcca
    12121 aattttactt tggtcatcaa ctgagatacc tagaccatta tctttaatag agaagacaat
    12181 ttgattatcg gactgtctta gactaataga aatatgattg tttgtaaatt taatagcatt
    12241 tgaaaataga ttatcaaaaa gccgtttgag tagtgtttgt tgacctataa tccaaaggtc
    12301 atcttcgatt tgagagtcaa ggtgtatagg ggtattatct aataatctac tcgaatcgga
    12361 tactagttgc ttcatcataa ttgagaaatt aagaggttct aactgaattg ataatggatt
    12421 ttctgcctta gtcaattcta gaagttgctc aactaatttt ttcattgata gtgactgacg
    12481 gtgtataacc tcaaaggatt cttttgcttc acttaaattt tctgcgtagt tttttccgta
    12541 ttcactttca gaaagtataa ctgttacagg agtccgtagt tcatgagaaa catcattgtt
    12601 aaattgtttt tcccgaacaa atgatgattc aatagaagcc aacatagtat tgataacagc
    12661 tgctagctca gttaattcat tttcgttatt tttagtgata attcgcttag tatagtcttc
    12721 attttgggta ataaattgag cggtttcagt aatttcttga atcggtctaa aagcgcgctt
    12781 taaaattaaa tagccaccaa cagttattat aatcaacata agaggaagaa cgatacccaa
    12841 actaaataac aattcagtta attgcttatc cagtctgtca acagtacgga tagcacgtaa
    12901 ccactgattt ttcccagaaa ttggaatatc aacatactga aaggtacgat tctccatgga
    12961 aaatagagat
//
LOCUS       DATROY010000049        12851 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.050, whole genome shotgun sequence.
ACCESSION   DATROY010000049 DATROY010000000
VERSION     DATROY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12851
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..658)
                     /locus_tag="VBD11_001083"
     CDS             complement(<1..658)
                     /locus_tag="VBD11_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6954043.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     Y"
     gene            complement(722..2068)
                     /locus_tag="VBD11_001084"
     CDS             complement(722..2068)
                     /locus_tag="VBD11_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6954044.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     regulatory      complement(2225..2357)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2665..3990
                     /locus_tag="VBD11_001085"
     CDS             2665..3990
                     /locus_tag="VBD11_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6954045.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     gene            4123..4509
                     /locus_tag="VBD11_001086"
     CDS             4123..4509
                     /locus_tag="VBD11_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6954046.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            4615..6894
                     /gene="pcrA"
                     /locus_tag="VBD11_001087"
     CDS             4615..6894
                     /gene="pcrA"
                     /locus_tag="VBD11_001087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6954047.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            7306..7551
                     /locus_tag="VBD11_001088"
     CDS             7306..7551
                     /locus_tag="VBD11_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6954048.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
     gene            7599..7847
                     /locus_tag="VBD11_001089"
     CDS             7599..7847
                     /locus_tag="VBD11_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6954049.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            7862..8443
                     /gene="amaP"
                     /locus_tag="VBD11_001090"
     CDS             7862..8443
                     /gene="amaP"
                     /locus_tag="VBD11_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6954050.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            8453..8647
                     /locus_tag="VBD11_001091"
     CDS             8453..8647
                     /locus_tag="VBD11_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6954051.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            8703..9245
                     /locus_tag="VBD11_001092"
     CDS             8703..9245
                     /locus_tag="VBD11_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6954052.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            9292..9489
                     /locus_tag="VBD11_001093"
     CDS             9292..9489
                     /locus_tag="VBD11_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6954053.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            9511..10062
                     /locus_tag="VBD11_001094"
     CDS             9511..10062
                     /locus_tag="VBD11_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6954054.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            10226..10867
                     /locus_tag="VBD11_001095"
     CDS             10226..10867
                     /locus_tag="VBD11_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6954055.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            10872..11636
                     /locus_tag="VBD11_001096"
     CDS             10872..11636
                     /locus_tag="VBD11_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6954056.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            11636..12295
                     /locus_tag="VBD11_001097"
     CDS             11636..12295
                     /locus_tag="VBD11_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6954057.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            12368..>12851
                     /gene="tpx"
                     /locus_tag="VBD11_001098"
     CDS             12368..>12851
                     /gene="tpx"
                     /locus_tag="VBD11_001098"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6954058.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     K"
BASE COUNT         4260 a         1947 c         2547 g         4097 t
ORIGIN      
        1 cgtattgttt taattgataa tcatcgaaac catccgataa gctgaaagca gattctataa
       61 aaatatcata agctgtttta agaataaaat aagtaataat gatagcagct aaacgatcaa
      121 taataggaaa atttagagaa gccgcaatga tagcaataga tgtgccgata gatgtcacag
      181 catcagaaag gttatcttta gaagctgcaa ccagagcgct agactttact ctttgagata
      241 attgtttatt atagaaacaa acgccaagca taacaagagc agatatagca cctactatag
      301 cacctagagg atcaatgtta gtatcagtat tattaataat cttagtgaca gtttgaataa
      361 aaacttgaat acctacgaca aacatgataa aagaggtaat caggcttgct aaatcttcaa
      421 ttttccagtg accaaaacga tggtttgagt cggctggttg actagccaag tgtaaaccaa
      481 ttaagagtgc tacatttcct aagatatcag ataagttatt aaatccatct gctacaagtg
      541 aagtagcatc aaaccaataa cctgcagcta atttagcgac tgccagtgtt atataggcaa
      601 tgatactaat aataggccct ctttttgcaa gttgtaagtt ttcagtagaa gttgccattt
      661 tcctaatttc ctttcatcta cacattatat cacttaggag cagactataa aactagtaag
      721 ttcaaggctt agtgatattt ttagtctctg aaaaatattt ctgtgtttct ttaacaataa
      781 ttggtgacaa agctaggaga gctattagat ttggtaaagc cattaaacca ttaacgatat
      841 cagcgataac ccaaatgaga tcgagctgta agaaaccacc taagccgacc ataatgacaa
      901 aaataatgcg ataagcgtta ataaatttag tccccaatag atattcaaag caacgctctc
      961 cgtagtagct ccatcctaaa atagtagtaa aagcaaataa gacaaggcag aaggttaggg
     1021 caaaactacc tagtgagcca aatacggtat taaaagatgc ttgtgttaac ggagcaccct
     1081 gtaaatcaaa ttctagccat tttcctgtaa ctaaaattgc aatccctgtc aaagtacaaa
     1141 taacaatagt gtcaatgaag gttcctgtca ttgaaataag cccttgttca acaggttctt
     1201 ttgttttagc tgctgctgca gctattggag cagaacccaa tccagattca tttgagaaaa
     1261 ctcctctagc aattcccttt tgaattgctt ctttaacgat agctccagaa aaaccaccaa
     1321 tggctgctgt cccagaaaaa gcaccgctaa agatgagttg gaaagtatgt ggaatcttat
     1381 tgtaattaac tgcaataata atcagagttg ctaaaatata gcttatagcc ataaaaggca
     1441 ctattttttc tgaaactttt gaaatagatt ttatacctcc aaatataata aataatacta
     1501 taacagcaag aatgaggctg gtaaatctag ttgatatttt gaaagtatgc tcaagagaag
     1561 aggcaattgc attgacttga gtgaatgtcc ctattcctaa aagagctact aagatacctg
     1621 ctgcagaaaa aaagactgct aatggtttcc atttttgtcc cattccattg acaatgtagt
     1681 acattggtcc cccagaaatt tctccattag tatcttttgt gcgatatttg atagctaata
     1741 atccttctgc gtattttgtt gccattccaa aaaaggcggc tacccacatc caaaacaaag
     1801 ctcctggtcc tccagattta atagccgtag ccacaccaac gatattacct gtccctaccg
     1861 ttgcagcaag agcagttgca agagcagcat aacttgaaat atctccttgt ccattatctt
     1921 ctcggaaaat caatttgaaa gctctagaaa gttgtctcaa ttgaacaaaa cctaagcgaa
     1981 atgataggta aatacctgtt ccgactaata aagcaagtaa aggtggaccc caaacgaagc
     2041 tattgatatg agtaaaaagt gttagcatat gttctccttt gatacatgac atacctatgg
     2101 attctgtaat gaaacgaaag aagttatctc taaaagagat aacttctgaa agatgctagc
     2161 taaaaagcgc acaaataaag gtagtagtaa acttaataag cttactactc attattaata
     2221 ttggctttta gcttctgtcc ttttgcctga gagattggag gagttgtttt actcttcttg
     2281 ccccttcggc gcctagagca catcttggtg tcaatacaaa gatgttgcta cttataggtc
     2341 tctccagaag tccgtctgtc aatcagtcct gtcttaagac acctgagagt gtaattcctt
     2401 cggcgaaagt ctaactttct tctcctgaat gacgtcattc ggtatatttt attatgttaa
     2461 ctatcataat gaaaaagcat agttttgtca atatagtttc aaaaaactct tgttttctaa
     2521 aatgtcttat gctataattc tttcagcgta ggtttttaaa ctcatcccgt gaggtgagaa
     2581 aaacatcagg aaaaaaataa agcctatgac tatattgcta tagaaagtgg gcttttttgt
     2641 atgcccaaga ggactagttt agatatgact ttaaatgcta aaggtgaaag taatcaaatt
     2701 ctagtagata aggaagtaca ttctactaac ttattactag atatcgatga aaaaccagaa
     2761 ttatttcaag gtttgctttt aagttttcaa catgtttttg ccatgtttgg agctacgata
     2821 ttagttccgc ttattttagg gatgcctgta tcagtggctt tatttgcttc tggttgcgga
     2881 acgctcattt atcaagttgc gacaaaattt aaagtccctg tttatctagg ctcatcattt
     2941 gcttatatta cagctatggc attagccatg aaacaaatgc atggcgatat ttcggctgct
     3001 caaacaggta ttctttttgt aggtctaatc tatgtcgttg tggcgacggt tattaagttc
     3061 gtaggaaatt catgggttga taagattttg ccaccaataa tcattggacc tatgattatt
     3121 gttattggtt taggtttggc aaactctgct gtaactaatg caggttttgt cgctaaaggt
     3181 gattggcgta aaatgctagt tgctgttgtt acattcttga ttgcagcttt tatcaatact
     3241 aaagggaaag gttttatcaa gattattcca tttttatttg caattattgg aggttatatt
     3301 ttatcaatca ttcttggctt ggtagattta tctccagttg aaaaagctgc ctggtttgaa
     3361 ttaccaaaat tctatttacc gtttaagaca ggtttatttc acagttataa actttacttt
     3421 ggaccagaaa tgcttgcaat tcttccaatc tcaattgtaa cgattgctga aaatatcggt
     3481 gatcatacag ttttaggtca gatttgtggg cgtaactttc taaaaaaacc aggtttaaat
     3541 cgtcttttaa ttggagatgg tttagcaacc gcattttctg ctcttatagg agggccagct
     3601 gaaactactt atggtgaaaa tacaggtgtt attggcatga cgcgtatagc ttctgtaacg
     3661 gttattcgta atgcagcttt tattgcaatc gctttttcat tctttggtaa atttacagct
     3721 ttgatctcta caattcctag tgcagtactt ggtggaatgg ctatattact ttatggtgtt
     3781 attgcaagta atggattaaa agtgttgatt gaaaatcgtg tcaattttgc tgaggttcgt
     3841 aatctgatta ttgccagttc catgttagtt ttaggcctcg gtggagcagt ccttgatcta
     3901 ggtgctctaa ctttatcagg aacagcatta agtgctatag tgggtattat attaaattta
     3961 atattgccaa aagaaagcat gaaatcataa caaaagagct cagagtatct gagctctttt
     4021 gttatgatag atcagttaaa aactttaaaa taaaaacgtt tacagttatg gtttccttat
     4081 tttctgctat aatggtcata gaaaaagtga ggttatagta gaatgaaatt caatttagaa
     4141 caagtaaaag tatttgagaa ttacgaaatt gaaaactggg aagaaggtca agttacctta
     4201 acaacgaagg tcgttgactc ttcgctcaac tattatggta atgctcatgg tggttatttg
     4261 tttacactgt gtgatcaagt cagtggttta gttgctattt caacaggata tgaagcggta
     4321 actctacaat caaatatcaa ctatttgagg gcaggccgat tagatgatct gcttactgtc
     4381 atcggtactt gtgttcataa tggtcgtact acaaaagtcg ttgatgttca cattaaaaat
     4441 cagaaagacg aaatggttac aactgctatt tttacaatgt ttgtaactgg agagagaaaa
     4501 gagaagtaaa aaaggtagga gttagacagc gtctgtttaa ctcctttttc tattgggaaa
     4561 aagatgaaaa aatgatatac tagagaggca attttgacaa aattaggagt agaaatgaat
     4621 cctttaataa ttggaatgaa tgataaacaa gcagaagcgg tacaaacgac agacggaccg
     4681 cttttgatta tggcaggagc tggctctgga aaaacacgtg ttctgactca tcgtattgct
     4741 tatttaatag atgaaaaata tgttaatcct tggaatattt tagcgattac ttttactaat
     4801 aaagcagcgc gtgaaatgcg tgaacgtgct atcgccttga atccagctac ccaagatact
     4861 ttgattgcaa cttttcatag tatgtgtgtt cgtattttac gtcgtgaagc tgattatatt
     4921 ggatataatc gtaactttac aatcgttgat ccaggtgagc agcgtacttt aatgaagcga
     4981 atcattaagc aattaaattt ggacacaaaa aagtggaatg aacgttcaat tttaggtaca
     5041 atctctaatg ctaaaaatga ccttcttgat gaaattgcat atgagaagca agccggagat
     5101 atgtatacac aggtaattgc taaatgctat aaagcttatc aagaggaatt acgtagaagt
     5161 gaggccatgg atttcgatga cttgattatg atgacacttc gattatttga tcagaataaa
     5221 gatgttttgg cctattacca acagaggtat caatatatcc atgtagacga gtatcaagat
     5281 actaaccacg ctcaatacca attagttaag ttattagctt cgcgtttcaa aaatatttgt
     5341 gttgttggtg atgccgatca atccatttat ggatggcgtg gagctgatat gcaaaatatt
     5401 cttgattttg aaaaggacta tccgcaagcc aaagttgtat tattagaaga aaattatcga
     5461 tcaactaaga aaatacttca agctgctaat aatgtgatca atcataataa aaatcgccgt
     5521 cctaaaaaat tatggactca aaatgatgaa ggtgagcaaa ttgtatatca tagagctaac
     5581 aacgagcaag aagaagccgt ttttgtagcg tcaactattg ataacatcgt tcgagaacaa
     5641 ggaaaaaatt tcaaagattt tgccgttctt tatcgtacga atgcgcaatc tcgtactatt
     5701 gaggaagcac ttttaaaatc caatattcca tatacaatgg ttggtggaac aaaattctat
     5761 agtcgtaaag aaattcgaga tgttattgct tatctcaata tccttgcaaa tacttctgat
     5821 aatatttctt ttgagcggat tgtaaatgaa cctaaaagag gggtggggcc aggaacttta
     5881 gaaaaaatac ggtcatttgc ctatgaacag aacatgtctc ttcttgattc ctcttcaaat
     5941 gtcatgatat cgcccttaaa agggaaggct gctcaagctg tttgggattt agctaatctg
     6001 attttgactc tacgtagtaa gctagatagt ttaactgtaa cggagattac agaaaaccta
     6061 ctggataaga caggatatct agaagcactt caagttcaaa atacattaga aagtcaagcg
     6121 cgtattgaaa atattgaaga attcttatca gtgactaaaa attttgacga taacccagag
     6181 attacagtag agggtgaaac tggtttagat cgtctatcgc gttttttaaa tgatttagct
     6241 ttaatagcag atactgatga tagcgccact gaaacggctg aagtcacttt aatgacatta
     6301 catgctgcca agggtctaga atttccagtt gttttcttaa tcggtatgga agaaggagta
     6361 tttcctttat cgcgtgcaat agaggatgct gatgaattag aagaagagcg tcgtttagct
     6421 tatgtcggga ttacgagagc agaacaaata cttttcctta ccaatgctaa tacccgtact
     6481 ttatttggta aaactagtta taatagacca acccgtttta tacgtgagat tgatgatgag
     6541 ctgatacaac atcaagggtt ggctcgacca gttaattctt catttggtgt gaaatattca
     6601 aaagaacagc ctacacaatt tggtcaagga atgagtcttc aacaagcgct tcaggcacgt
     6661 aaaagtaatt cacaacctca agtaactgcc caactccagg ctcttaacgc gaacaatagc
     6721 catgagacat catgggagat tggtgatgtt gcaactcata aaaaatgggg ggacggtacg
     6781 gtgcttgaag tttcaggtag cggaaagact caagagttaa aaataaattt tccaggtatt
     6841 gggttaaaga aattattagc gagtgtcgct cctattagta aaaaggaaaa ctaactttct
     6901 tctattatat gcttaaataa tcagagacta agaattgtat tcttagtctt tttgcatcat
     6961 ttgaatagac aaaataagtt atcatttggt ttttacttac taaaaaggtt gatattaaag
     7021 cgttttccaa aagtgaccat actattttaa agaaagaagc gtttctcttg cattcttttc
     7081 taaaaagagt ataataacaa tatcaattaa atatcaagtg gtcacttaaa aataaaagca
     7141 tttaccaagc tttttacttg gcagtcactt tataaagttg tataatatta gtatagttaa
     7201 caacttaatt aattgccgag tggtcacttt agaaactctt gttatttagt taagaacaaa
     7261 ataagtttgt acactgagat gtacttagga agaaaggtat ttatcatggg gttaatttgg
     7321 tcattaatcg ttggtgctat tattggtgct attgcaggag caatcactaa caaaggtggt
     7381 tcaatgggtt ggatagctaa catcttagct ggtttagttg gttcatttgt tggtcaaagt
     7441 ttactaggaa catgggggcc taagttagca gatatggctt tgattccttc aatcgtaggt
     7501 gcgattatcg ttatcattgt tacttcattt gtattaggca aaaaatcata actacataac
     7561 tagtagaatg attagtcaag aaagagaagg tatttatcat gagtttaatt tggtcattaa
     7621 tcgtaggtgc tattatcggt gctattgcag gggctgttac taacaaaggt ggttcaatgg
     7681 gttggatagc taacatctta gctggtttag ttggttcatt tgttggtcaa agtttactag
     7741 gaacatgggg gcctaaatta gcaggtatgg ctttgattcc ttcaatcgta ggtgcgatta
     7801 ttgttgtcat tgttacttca tttgtactag gtaaaatgaa taactagaaa aggagttacc
     7861 tatgtcaaaa ggactaaaaa gtttatatac tttgctaggt ctaatctccc ttaccctact
     7921 aggatttgta gctgttatca gtaaacaaca catttatcta ccatctttta attggttaga
     7981 ttgggatttt aatttgccat caccgattga cgttggtatg tatcattatt tcttttgggg
     8041 agcattagtg ctgtttgtaa tcgttttact ggctatattg gtagttctgt tttatccaag
     8101 acgatacaca gagtataaac tggctgataa aactggtaaa ttgatgttaa aaaaatctgc
     8161 aatagaggga tttgttaaaa cagaagtatt aaaaactggt ttgatgaaat ctcctagtgt
     8221 gacagctcat ttatataaga aaaaagtgaa agttgatgtt aaaggtcttt taacttctcg
     8281 tacaaatgta ccggaacaac ttgaacatat tcaaagtgat gttgaacttg gcttaaaaga
     8341 attctttggg ttggaaaaga aaatgaacac tagagtattt gttaagcaag ttgaagaaga
     8401 aaatgttgga aatgctaaga cgaataaaag tcgtgtagaa taggagtcta atatgtcaga
     8461 attcgtgagg aaatacagat atccactagg aggtgcagtt attgggctcg ttttagctgc
     8521 aatgattgta accattggat tctttaaaac aattcttgct cttgttatca ttgtgctagg
     8581 tgcttatgct gggctttatg tgcaaagaac aggtatgctt gaccaattct ttaataagag
     8641 aaaataaaat ttttcgttta tatttattct acattattta aataaaagaa agaggaacta
     8701 ttatgactga aacttacatc aaaaacacaa ctaacaattc tggcacgaca gctgttcgtg
     8761 gagaattaac tttcgaagat aaagtgattg aaaaaattgt tggaattgcg attgagcatg
     8821 tagatggtct tttggctgta aatggtggtt ttttctcaaa tttaaaaaat agcgttgtaa
     8881 attctgattc agttacagat ggcgtaaatg ttgaagtggg taaaaagcaa gttgctgttg
     8941 atttggatat tgttgctgaa taccaaaaac atgttccaac tatctttgcc gatatcaaaa
     9001 aagttgttga agctgaagtg aaacgtatga cagaccttga agtggttgaa gttaatgtta
     9061 atgttgttga tattaaaact cgtgctcaac atgaagaaga ttcagtaact cttcaagatc
     9121 gtgtaacaag tgcagcacaa gcaacaggtg aatttgcttc gaatcaagtt tcaaacgtta
     9181 aaagtgccgt aggtaacggt gttgataaag tggaagacat gaagtctgaa ccacgtgtac
     9241 aataattgat ttaagtatag tttaaagtat tgttattagt aggaggctat tatgtctgaa
     9301 gaaaaatttg atgcaaaagt tgataaggtt tctggttcag taaaagaatc tgttgggaaa
     9361 cttactggcg ataaagaagt tgaatctgaa ggtaaagtcg ataaattgaa aggtcatgca
     9421 aaagaaaaac ttgctgacat taaagatacc attaagggtg cttcagagag ctttaagaaa
     9481 aaggactaga aggtttgagg tcatttattt atggaaaatc aaaaacaaga tattaaaact
     9541 tcagtaactt atgaagaaaa ggtaattgct aaaattgttg gacatgcttt agaatcagta
     9601 gacggactct tggctgtgag tggaggtttc ttctctaatc taaaaaatag cgttgtaaat
     9661 tctgactcag taactgatgg tgtaaatgtt gaagttggta ctaaagaagt tgcagttgat
     9721 ttagacatcg ttgttgaata cggcaaggat atcccagcta tcgttgaaag cattaaagcg
     9781 attgtcagtc aaaacgttga agtgatgacc caccttaaag ttgttgaatt gaacgcaaat
     9841 gtcgtagatg ttaagactaa agctgaacat gaagctgact cagtgacagt acaagatcgt
     9901 gtatctgatg cagcacaagc aacaggaaac tttgcttctg aacaagctgg caaagctaaa
     9961 gcagcaattt cttctggagc agagaaaaca aaagaagcag tatcaaatgg tactgaggct
    10021 gcaaaagaaa aaatctcaga agctcgtact agtgaatctt aattcagtaa actacttttt
    10081 atgataagac actcggagct gggagaccag ctccttttat atgatattta ctaaagacat
    10141 tagatattaa caaaaaagat atatttgcaa tattttgcta attgtggttt aatatgtatg
    10201 tttttattag gataaggagg gttggatggt ttctgaacaa tctgaaattg taacatctaa
    10261 atatcaaaaa atagcagtag cggttgcaca aagaatcgca aatggagatt atgaagttgg
    10321 agagaagttg aaatctagaa caactattgc ttcaacattc aacgtatcac cagaaacagc
    10381 acgtaagggt cttaatattt tagcagattt acagatttta actttaaagc atggcagtgg
    10441 agccattatt ctttctaaag aaaaagcaat tgcattctta aaccaatatg aaacctctca
    10501 ttcagttgct attttgaaag gtaaaatccg tgataatatt aaggctcagc aacaagaaat
    10561 ggaagagtta gctactttag ttgatgattt tttgcttcaa acacgtgcgg ttagtaaaca
    10621 atacccacta gcaccatatg aaattattgt cagtgaagat tctgaacacc ttggtaagtc
    10681 aataggagaa ttaaatgtat ggcatcaaac tggtgcgact attgtggcta ttgaacacga
    10741 agggaaattt attgtttctc cagggccttt ctcagttata gagcaaggag atcatatctt
    10801 ttttgttggt gatgaagatg tttatgcacg tatgaaaacc tattttaact taaggatggg
    10861 gctttgagag aatggatttt gtctttgata tagatggaac tctttgtttt gatggtatga
    10921 gtctatctaa agaaatacaa ggaatactag agagagccca gattgattat ggtcatagag
    10981 tcacttttgc tacggcgcga tcttatagag acactatagg tatccttggc gacaagctat
    11041 ctttatcaaa aattattggt ttaaatggtg cgactttaca tgaaaatggt cacttagtgg
    11101 atagttacta tttacaaagt gactttttta gtactattat ttcatattgt catcgctatc
    11161 agattccata ttttgttgat gaggtcttta attacgctac ttatcaggct tctaaaattc
    11221 cctttattgc ttatgttgat cctcaaaaaa ggggagagct tctagaagtt tctaaaattg
    11281 agaaaccgat aaaaatggtg ttgtattttg gtgatcagct gagaagggca gaccaaatgt
    11341 tagcgaaatt aaatcgtttt ggacttagta gtcacttttt ccacgaattt gaaaaatgtt
    11401 tgtacataaa tcccattgca gttgacaagg gaaaagccac taagaaatta tttgggaatc
    11461 gttttatagc ttttggcaat gataaaaatg atatttctat gtttgatgct gcgcattata
    11521 gtgttcaagt aggggatttt gatgaactga ctccttacgc taatttacgt gtatcaagag
    11581 aatctgtaca tgaaggaatt acaatgcttt ttgaaaaata caaggggatt acgtaatgga
    11641 tttattccta agtttagaga ggaaatttaa agctgcaagt gacaaagaag tttctaagca
    11701 acaggaggct tatttacgcc atcattttaa gtgctatggt attaaatcac ctgagcgacg
    11761 tatgctctac aaagagctta taaaggccgc taaaagacaa gcaaaaattg attggcaatt
    11821 gcttgataaa tgttggcaaa gtgactatcg tgaatatcat cattttgtac ttgattatct
    11881 attagctatg agtcaatttc tcacctataa tgattgctct agactagagt tttacgctcg
    11941 tcatcaacag tggtgggata gtattgatgt tctgactaag atttttggga acttatcctt
    12001 aaaagatgac aaggttatga atttactgtc agaatggtca cttgatcaag atttttggat
    12061 gaggcgacta gctattgagc atcaactagg atttaaagaa aaaacaaata ctgatatttt
    12121 gtcacttttt attttaagaa atacaggaag tcaagaattt tttatcaata aggcgattgg
    12181 ctgggcgctt agggattatt caaagtataa taaagtatgg gtaaaggact ttatcagtaa
    12241 tcattatgat gagttgtcga ctttgagcat acgcgaagga agtaagtacc tctaattatc
    12301 agttcaatta ttcgaaaaag aagaagcttc atagtaaaat aaaggtaatt gataaggaga
    12361 taaacttatg acgacatttc ttggaaatcc tgttactttt acaggaaaac agctacaagt
    12421 tggggatatt gcaaaagatt ttttactaat agctacagac ttatctcaaa aatctttaaa
    12481 agattttgaa gggaaaaaga aagttattag cgttgttcct tctattgata caggtatttg
    12541 ttcaaaacaa acacgtactt ttaatgagga attatcagag ctagataaca cagtggttat
    12601 tactgtttct atggaccttc catttgctca gaagcgttgg tgcagcgcta aaggtcttga
    12661 taacgtgatt ttattatcag atttttatga tcattcgttt ggtcaagagt acgctctttt
    12721 gatgaatgag tggcatttgt taacgcgtgc agttcttatt ttggatgagc ataataaggt
    12781 gacttacacc gaatacgttg acaatgttaa ttcagatgtt gactacgaag cggcgattaa
    12841 tgctgcaaag a
//
LOCUS       DATROY010000050        12829 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.051, whole genome shotgun sequence.
ACCESSION   DATROY010000050 DATROY010000000
VERSION     DATROY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12829
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            24..464
                     /locus_tag="VBD11_001099"
     CDS             24..464
                     /locus_tag="VBD11_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6954059.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            511..2328
                     /locus_tag="VBD11_001100"
     CDS             511..2328
                     /locus_tag="VBD11_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954060.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVELFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            2318..4072
                     /locus_tag="VBD11_001101"
     CDS             2318..4072
                     /locus_tag="VBD11_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954061.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            4180..4806
                     /locus_tag="VBD11_001102"
     CDS             4180..4806
                     /locus_tag="VBD11_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6954062.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            4808..5494
                     /locus_tag="VBD11_001103"
     CDS             4808..5494
                     /locus_tag="VBD11_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6954063.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYIFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            5494..6723
                     /locus_tag="VBD11_001104"
     CDS             5494..6723
                     /locus_tag="VBD11_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6954064.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            6839..7717
                     /gene="mvk"
                     /locus_tag="VBD11_001105"
     CDS             6839..7717
                     /gene="mvk"
                     /locus_tag="VBD11_001105"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6954065.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            7699..8643
                     /gene="mvaD"
                     /locus_tag="VBD11_001106"
     CDS             7699..8643
                     /gene="mvaD"
                     /locus_tag="VBD11_001106"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6954066.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            8636..9628
                     /locus_tag="VBD11_001107"
     CDS             8636..9628
                     /locus_tag="VBD11_001107"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6954067.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            9625..10620
                     /locus_tag="VBD11_001108"
     CDS             9625..10620
                     /locus_tag="VBD11_001108"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6954068.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            10688..10906
                     /locus_tag="VBD11_001109"
     CDS             10688..10906
                     /locus_tag="VBD11_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6954069.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(10952..11806)
                     /locus_tag="VBD11_001110"
     CDS             complement(10952..11806)
                     /locus_tag="VBD11_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6954070.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(11907..12821)
                     /locus_tag="VBD11_001111"
     CDS             complement(11907..12821)
                     /locus_tag="VBD11_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6954071.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
BASE COUNT         4185 a         1990 c         2578 g         4076 t
ORIGIN      
        1 ataactatta aaggagtatt attatggaga atcctcttca aaaagcacga atacttgtta
       61 atcaacttga aaagtattta gatcgttatg caaaagagta tgatgttgaa catttagcgg
      121 gtccacaagg acacttagtg atgcatctct ataaacatcc tgacaaggat atgtctataa
      181 aagatgctga agaaatttta cacatttcta agtctgtagc gtctaatttg gtaaaacgga
      241 tggaaaaaaa tggatttatt gcgattgttc catctaagac agataagcgt gtgaaatacc
      301 tttatttgac tcatctaggt aaacaaaagg ctacacaatt tgaaattttc ttagaaaaat
      361 tgcacagtac catgttagca ggtattacta aagaagagat acgtactacc aaaaaggtta
      421 ttagaacatt agctaaaaat atggcgatgg aagattttga ttaactattt taggataata
      481 ggataaatat gaaagtaaaa ggagtttgcg atgtttaata ttttcaagcg actcaatggt
      541 aaagaatggt tgatgattgt ggcgagtact atattagtgt gtctagcggt ttggatggac
      601 ctaaagactc cagaatatat gtctgatatc acacgtatgt tgcaaactaa aggtacaaag
      661 ttagaggctc tttgggatcc tggactaaaa atgcttgctt tttcatttgg tagtttttta
      721 gtagcagtaa tggtaggttt tttaggtgct aggacagcag ctagttttac tagtcgatta
      781 cgttcagata tttttaacca tgtgatggat tattctgatg cagaaattaa gcgattttct
      841 atcccctctc tattaacgcg cacaactaac gacttgactc agctacagat ccttattgta
      901 atgggaatgc aggttgttac ccgtggacca attatggcaa tatgggcctt gactaaaatt
      961 gggggtaaaa gcgatcaatg gactggggca gttggagtag cggttattat cgttatgata
     1021 ttacttagcg ttctactctt acttgctttt ccgagacaac gtcaaatcca aggttttaca
     1081 gatgcgctta atgcaactac tagagaggca ctctctggtg ttagagttat tcgtgcctac
     1141 aatgctgaaa agtatcaaac agataagttc aaacgtgaaa atgacgattt aacaaaattg
     1201 aacctttttg tttatagatt gatgtcgctt atgaatcctg tgatgacagt ggtttcatct
     1261 ggattgactt tagctattta ttggattggc tcttatttga ttaatgatat ttctgtgcct
     1321 aaagttacag gaacaagtgt atctgtattg gctgctaaat cagctatcac tgatcgagtg
     1381 gaactttttt caaacatggt tgttttttca tcttatgcta tgcaagttgt tatgggattt
     1441 atgatgatgg tagctatttt tatcatcatg cctcgtgctt tagtatcggc tagacgtatt
     1501 aatgaggtac taactttagc cccaacagta acttttaaag aaaatagtca tatccaaagt
     1561 gcaccaaaag gtcaaattga gtttcaagat gtgtcatttc attatagtaa agattctgaa
     1621 gcggttattg aacatgtatc tttcaaagca gatactggtc agacagtggc atttattggg
     1681 tctacaggtt ctggaaaatc cactttagtc aatttagttc cacgttttta tgacgccaca
     1741 gaggggtcaa ttaaacttga tggcattgat ataaaggaat actctcacaa tgatttgaat
     1801 aataaaattg gctatattcc acagaaagct gttctcttta gtgggacagt acgttctaat
     1861 atgatttttg gagagagtgg cagggaggaa cttagtgatg aacaaatttg gcaagcactc
     1921 gaattagcgc aagctaaaga atttatagaa gctaaagaaa aaggattaga tacagaagtc
     1981 gctcaaggtg gtagtaactt ttcaggagga caaaaacaac gcttggcaat cgcacgtgct
     2041 ttagcacgtc accctgaaat tctcattttt gatgactcat tttcagcgtt agattataaa
     2101 acagatcata ttttacgcca tgtgctgaaa gaaaaaacag ctgctatgac gaaattaatt
     2161 gtcgctcaac gcatttcaac tatcatggat gctgatctca ttttagtgct agatcaaggt
     2221 aaagttgttg ggcaaggaac acataaggaa ttgttatcaa ataatgaagt atatcaagag
     2281 atagcctatt ctcaattgtc taaggaggaa ttggaaaatg aaaactaaat cacaaaaaac
     2341 gtcttttttc tctaatttat ctccgtacct caaaggttac aaacttcttt ttgcgttagc
     2401 aattgtgtta gcaactttat caagcttgac aacagttata ggaccagacc gtcttaaaga
     2461 gatgacggat cttatggtaa aaggcttggc tggtaatatt gacttagcca aaatcggtca
     2521 tattgcaggg caattggcag tattatatgt tttaggtgca ttagtatcct atctagcaag
     2581 ttttattgtg acgactatta ttcaaaaatt tgctcaaggt atgcgcaatg ctattgctga
     2641 taaaatcaat aaaatacctt tgagttattt tgatagccat actcaaggtg atactttatc
     2701 tcgtgttaca aatgatgtgg atttgatgac acagtctttt aaccaaagtt tagttactat
     2761 ggttgcatct acagtgttat tggttggttc attaattatg atgttcaaaa cagattggca
     2821 tttagcaaca acggcgatta tatcggtatt ttcaggattc gctctttctt caatcattat
     2881 ggtgaagagt cagcctttat ttaaaaagca acaggtgaat ttggctaata tatctggata
     2941 tgtagaagaa atttactcag gacacaacat tgttactagt cataatgcaa gacagcaggc
     3001 taagtcagat tttcaactat taaatgataa acttcacagt tccatgtgga aatcacaatt
     3061 tttctctgga attatgatgc ctctgatgca gtttattggt aattttggtt atgtgatggt
     3121 gtgtgtggtt ggtgccatgt tagctatcaa aggaacaatt actatgggta caattgttgc
     3181 ttttatgacc tatgttcgta tttttacaca gcctattggt cagattgctc aaggaattac
     3241 acaattacag tctgctagtg cagccatggg acgtgttttt gaatttttaa atgaaacaaa
     3301 tatgcaagag gataaacaca aagtgttaaa tcttcctaag tttaaggggc atgtgtcctt
     3361 tgaacatgtc aagtttggtt atagtgataa caagactatt attcatgact ttacttcgga
     3421 ggcacagcct ggtcagaaag ttgctattgt aggaccaact ggcgcaggta aaacgacaat
     3481 tgttaattta ttgatgcgtt tttatgatgt taattcaggt agtatttcca tagatgacat
     3541 caatatccaa gacatgaaac gtagtcaagt tcatgatgct ttctcgatgg ttcttcaaga
     3601 tacatggttg tttgagggaa ctattaaaga aaatttgatt tttaaccaga cgacaatcag
     3661 tgatgatgag gttcaagaag ctgctaaggc agtcggtata catcatttta taatgacact
     3721 acctaaaggg tatgatactg tccttgatga cgctgtgaca ttgtcagttg gacaaaagca
     3781 attattaacc attgctcgag ctcttttgaa aaattctccc ttacttatct tagatgaagc
     3841 aacttcatct gttgatacac gtacagaaga gttaattcaa aaagctatgg acaaacttat
     3901 ggaaggaaga acatcatttg ttatcgcaca tcgtttatca acgattaaaa acgcagactt
     3961 gattttggtc atgaaagatg ggaatatcat tgagcaaggc aatcatgatg ttttgatgtc
     4021 tcaaagtggt ttttatgctg atctgtataa ttctcaattc gaatcagctt aacataaaca
     4081 ttttagaagg aaatgggaag aaaaaattct cattcctttt tagtttgcct taagtttcta
     4141 tgatataatg gagcttaaga gagaatagga aggtttctta tggaaacaaa tatttatgga
     4201 gattatggtc gatatctacc attaatttta gaagatttca gtcagcgtat ccaattagaa
     4261 aatgataaag ctaaggttga aacaggctat aaattgtacg aacatatcat tggtcgtatt
     4321 aagacatcag atagtatgat agagaaatgt cgtcgtaaac agttacctgt aacagtagat
     4381 tctgcactaa aagttattag agatagtatt ggagtccgta ttatttgtgg ttttgttaac
     4441 gacatttatc aaattgtaga acgtattaag acatttgatg attgtcgtat tgtagttgaa
     4501 aaagactata tccagcatgt taagccaaat gggtatcgct cttaccatgt gattttagaa
     4561 attgatactc cctatccgga ctgtttgggt aattcagaag gtaaatatta catggagatt
     4621 cagttgcgta caattgcgca agattcttgg gctagtttag aacatcagat gaaatacaag
     4681 catgatattg aaaatcccga acgaattgta agagaattaa aacgttgtgc tgatgaaata
     4741 gcatctgttg atttaacgat gcaaacgatt cgtcaattga tagagagtgg aacaaagaag
     4801 gaataaaatg agagtattaa ttgcagaaga tgaagaacag atgtcacgtg tccttagtac
     4861 ggcaattagt catcaaggtt atgttgttga tgtcgcctac gatggtcaga ctgctattga
     4921 tttagctaat caaaatgcat atgatgtcat ggttatggat gtcatgatgc ctgttaaaac
     4981 aggtgttgag gctgtcaaag aaattagaca atcaggaaat aaatctcaca ttattatgct
     5041 aacagccatg gcagaaattg atgatagagt aacaggtcta gatgctggtg cagatgatta
     5101 tttaacaaaa cccttctctc taaaagaatt attagcaagg cttcgctcta tgtcccgaag
     5161 attggaagat ttcacaccta atgtattgtc attgggaaga gtaaccttat cagtcggtga
     5221 acaagagttg caatgtgaaa acactattcg tctagcgggt aaagaagcta aaatgttagc
     5281 atttttcatg ttgaaccatg ataaagaatt atctacacaa caacttttcg aacatgtctg
     5341 gggagcagat aaggaccaag aagatgtaga tgagggatac atctttatct atgtttctta
     5401 tttgagacaa aaattgcagg caattggtgc taacttggag attattggtc aagaaggtag
     5461 tagctataaa ttaagtgaaa taaatggtgg ctaatgttta gaaatttacg acttcgtttt
     5521 atcggaatag ctgcgctagc tattcttgtt gtcttatttt cagtagtcgg tgtcctcaac
     5581 tctgctaatc actatcaaac gaagaatgaa atttatcgtg tgttaactat ccttgcagac
     5641 aacaatggac gtataccgaa taaacttgaa tttagtaaag aattaggtga tgacctatca
     5701 acggacgcta ttttccaatt ccgttatttc agtgcgcgaa cagatgcaaa aggtaacgtg
     5761 actagttttg attctagaaa tatctttgaa gtaagcgata ggcaaataaa aaattatgcc
     5821 aagcgtatcg tatcacaaaa tagtcattca ggtcatatca cctataattt tagtacttat
     5881 tcttatctac taaaaaaggt tggtaaaaat gattatttag tggtattttt agatacaacc
     5941 aaccaatatc ttgataatca aagattattg caactttcaa tatggatgtc cttggtaagt
     6001 tttatcgtct tcatggtaat tgtttcagtt ttatcaggga gagttatctt accatttgtt
     6061 gctaactatg aaaagcaaag gcgttttatt accaatgcag gacatgaact aaaaactcca
     6121 cttgctatca tctccgctaa taatgaattg gtggaaatga tgagtggtga atcagagtgg
     6181 actaaatcta caaatgatca aatccaacgt ttaacgggtc taattaatgg catggtcagt
     6241 cttgcccgct ttgaagaaca gcctgatata agtatggttg acttagactt ttcacatatt
     6301 acaaaagatg ctgcagaaga ttttaaaggt ccaatcatta aagatggtaa agactttata
     6361 atgagtatcc aaccaggtat tcatgtcaaa gctgaagaaa aatcattatt tgaattggtt
     6421 acattattgg ttgataatgc taataagtat tgtgatccta tggggactgt gacagttaag
     6481 ttatcacgtt caagtcgttt gagacgagct aagcttgaag tttcaaatac ttataagaat
     6541 ggtaaagaca tagactatag caagtttttt gaacgatttt atcgagagga tgagtcacat
     6601 aataacaaaa aatcgggata tggtattggt ctttctatcg taactagttt ggtgcatcta
     6661 tttaaaggaa gtatcgatgt gaattataaa catgatacca ttacctttgt catttacatt
     6721 taagattaaa gggactgcaa ttgtagttcc tttaatttat gttattatca cgttaaagtc
     6781 gcttttttta aggatttata ttaaaatagt aaggtaatag aattgtaaag agaaaaagat
     6841 gaaagaaaag tttggtatag gaaaggctca tagcaagatt atcttgatgg gtgagcactc
     6901 agtagtatat ggttatccag ccattgctat tcctttaaaa aatattgaag taacttgcct
     6961 tattgaagag gctcctcaat taatagcatt agatatgact gatcctttgt cgacagccat
     7021 ttttgctgcc ttggactatt taggtaaaac ttcttctaaa attgcatatc atattgagtc
     7081 acaagtccca gaaagacgtg gtatgggatc atcagctgca gttgctattg cagctattcg
     7141 tgcagttttt gattattttg atgaagattt ggaggcagat ttgttagaat gccttgttaa
     7201 tcgagcagaa atgatagcac attcaaatcc gagtggatta gatgcaaaaa cttgtcttag
     7261 tgaaaatact attaaattta tccgcaatat tggtttttca acagtaccca tgcatttgaa
     7321 tgcttattta gttattgctg atacaggaat tcatgggcat actaaagaag cagtggataa
     7381 ggtgaaatca tctggcgagg cagtactacc ctttttaaaa gaattaggtt atttagcaga
     7441 ggcatcagag gatgctattc ataaaagtga ttccaaacaa cttggtagct tgatgactaa
     7501 agcacatcaa agtttaaaac aattgggtgt ctcaagttta gaagctgatc atctggtaga
     7561 ggtagctatt agttgtggtg ctctaggcgc taaaatgagt ggcggcggat taggtggttg
     7621 tattatcgca cttgtaaaag aaaaaagaga agctgagcgc ttaagtcagc aattagaaag
     7681 agaaggagct gtcaacacat ggacggaaaa agtataagtg ttaagtctta tgctaacatt
     7741 gctattatca aatattgggg aaaagcagat gctgaaaaaa tgattccagc taccagtagt
     7801 atttctctaa cacttgaaaa catgtacact gaaacacgtt tgactgccct tgggaaggat
     7861 gctaagaaag atgaatttta catctctggt gttcttcaaa atgatcatga acatgacaaa
     7921 atgtcagcta tcttggatcg ctttcgacaa aataggtccg gctttgtaaa gattgaaaca
     7981 actaataata tgccaacagc tgcaggctta tcatcgtcat caagtggttt atcagcctta
     8041 gtaaaggcct gcaatgattt ttttggtact aaccttagcc aatcacagct agctcaagaa
     8101 gccaagtttg cttcaggctc ctcttcacgc tctttttttg gacctgttgc tgcttgggat
     8161 aaagatagtg gtgatatcta taaagttcac actaatttag atttagctat gattatgctg
     8221 gtcttaaatg acaaaagaaa gccgatttca agccgtgaag gtatgaaaat ctgcaccgaa
     8281 acttcaacta cttttaatga atgggtaagg caatctgaac aagattatca agatatgtta
     8341 gtatacctaa aaaataatga ttttcaaaag gtaggtcaac tgacagagcg taatgcactt
     8401 gcaatgcact caacaactaa aacagcaact ccagcctttt catatttgac agaagagaca
     8461 tataaagcta tggatgttgt gaagaaactt cgcgaaaaag gtcatgaatg ttattatacg
     8521 atggatgcgg gtccaaatgt taaagtactt tgcttacgtc aagaccttga ggcattagct
     8581 gctatcttgg agaaagatta tcgtattatt gtttcaacga caaaggaatt agcagatggt
     8641 taaggtgcaa acaggtggca aattatatat tgcaggtgaa tacgcgatac tgtatcctgg
     8701 gcaagtagct attttaaaga acgttcctat ttatatgact gcattagcta cttttgcaga
     8761 taactacagc ctttattctg atatgtttaa ctatacggca agcttacagc cagataagca
     8821 atatagtctc attcaggaaa cgatattgct tatggaagaa tggttgatta attttggcaa
     8881 aaatataaaa ccgattcacc tcgaaattac gggaaaatta gagcgctatg gacttaaatt
     8941 tggtataggc tctagcggaa gtgttgtcgt tttgactatt aaagctatgg ctgcgcttta
     9001 tgagatagag atgccaagcg acttgctttt taaattatct gcctatgttc tcttaaaaag
     9061 aggtgataat ggctcaatgg gagatattgc ttgtattgct tatgagcatt taatttctta
     9121 ttcagctttt gatcgtaggg ctgtttcaaa aatgattgaa acaaaacctt tggaacaagt
     9181 attggaagct gaatggggtt atcgtattac caaaatacaa gctttattgg agatggattt
     9241 tttagtggga tggacaatgc aaccgtccat ttcaaaagaa atgataaata tagttaaatc
     9301 aactattacg cagcgttttt tagatgatac aaagtatcaa gttgtccagt tactttcagc
     9361 ctttaaagaa ggagataaag aagcgattaa gagatgttta gaagaaatta gcttgctatt
     9421 atttaatctt catccatcta tttacaccga taaacttcaa aagcttaaag aggcaagcaa
     9481 aggcctagat atagtcacaa aatcttcagg atctggagga ggcgattgtg gtatagccat
     9541 atcctttaat aaaaatgata atcaaactct aatcaagcgt tgggagagtg ctggtattga
     9601 actacttagt aaggagacac tgtcatgaca aatcgaaaag atgatcacat aaaatatgct
     9661 cttaaatatc agtcgcatta taatagtttt gacgatattg agttaataca ttcatcctta
     9721 ccaaaatata atgtcaacga tatcgattta tcaacacatt ttgctggtca aagctttgaa
     9781 tttccttttt atatcaatgc tatgacaggc ggtagtgaaa aagggaaagc agtcaatcat
     9841 aaattggcac aggttgctca agctacaggg attgttatgg tgacaggttc atattcggca
     9901 gctctcaaaa acgatgaaga tgattcctac ccaacaacag acttgtatcc tgatttaaaa
     9961 ctggcgacaa atatagggtt agacaagcct gtaccagcag cagagtcaac cgttaaggct
    10021 atgaacccca tttttttaca agttcatgtt aatgtgatgc aagaactttt gatgcctgaa
    10081 ggagagcgtg aatttcacat gtggcgttct cacttaaaag aatacgtcga caatatccaa
    10141 tgcccactta ttttaaaaga ggttggtttt gggatggatt tacaatcaat aaaggatgct
    10201 tacgatatag gtatcacaac tgtagatatt tcggggcgtg gggggactag ttttgcttac
    10261 atcgaaaatc aacgtggacg cgaccgttcg tatctcaata cctgggggca aacaacagct
    10321 caatccttaa taaacgctca atctatgatg gacaaaatgg atatcctagc atctggtggc
    10381 attcgtcatc ctttagatat ggtaaaatgt ttagtgttgg gtgctaaagc tgttggactt
    10441 tctagaacag ttttagaatt agttgagcgt tatcctgtag atgatgttat tgcaattctc
    10501 aattcttgga aagaagattt aagaatgata atgtgtgcac tgaattgtaa aaaaataact
    10561 gatttacgac aagtcaatta tatattgtat ggtcaattga aggaagccaa tgctaaatga
    10621 tattattttt gtgaagtaga tatagcttcc tgtcaaagtt tccttgtaaa acgtcagagg
    10681 taataagatg attaaactgt attataaaga tgcttacgac actgaacgat gccaagtttt
    10741 tgaaaatcca aatgatttta ttcgagtttt aatgggatgt gttactgttc ctgacaatta
    10801 tcctattacg aaaatgactt ataaagataa agatattgct tacagtggta cttatggaga
    10861 tctcttcaag acttttattg aatttgattg gtccactttg gactagtata gctaaaaagc
    10921 taaggctgtg aattgcctta gctttttagc tttatttttc ccattttgcc atatttggac
    10981 ctgttgggac gatggtatga tgacgaacag ctcttccaaa tttgcctaag taatagttat
    11041 tttttatgga ttgtggattg gttaatgttt ttaaattaaa gtcattgtat aaagttttag
    11101 cccaagaaaa aatatttggg tagtcttcta gcgctttctg attaagtagt agaagatcac
    11161 gcccagcaat atcccatcgg actaatggtg taaagaaatg aatatcaacc tcacttagat
    11221 tatctccgag caagaaagga cgttgtccta gtaagtgttc tttttgatct agaaaagtga
    11281 agaattcctt actataagta tcataatctg cttgtttatc aagaaaagct aacttataca
    11341 ttaagctttg gaagttctta ttaatgtctt taatgtcatt atcaataagt gaagcagttt
    11401 cttggctgaa aaggacagga gcatcctctt gatgcaaatc agagaaatct gtagtaaaaa
    11461 gtcgaagcaa atctgcagat tccttacaga caatacgatt atcggtcttg tcaattaaaa
    11521 ttgggatagt ataaggacct tggtaggtcg ggtcagtcaa atgataaata tcacgaagcg
    11581 acttagcacc agtgacctgt tctggttggc taaaaataag tcctatatca cttggaacat
    11641 catccacaaa actgatagaa atatgtttat ccaaaccaag cagttggcgc gcaatctgcg
    11701 gacgttgtgc ataaggacaa aattccccaa caatcatatg gtagcgacct gattctactg
    11761 ggacttccca ctcttggtca ataaatttag cagtaaatgt tgtcatcgta tacttccttt
    11821 caatgatatt tattgcttta agtataacaa aaaagaacct agacagatag tatttactct
    11881 aatacttatg tctaggtcct tttatattat ggtaatgagt taggaatgat aaactttaag
    11941 gctttaggga tgacttcaac ttttacaggt aattggtcac ctggatcgcc atcaatacga
    12001 actttaggga tagctgattt tttacgttta aaagttgaaa tagtcagatt attggtatga
    12061 aaatgtttta tctcctttgc ttgactaaaa tctcccttac gaaattgtct tagatgcaac
    12121 aataatttga agaagtgaat atgttccata gtgtagactc taaaaagtcc atcatcaatt
    12181 gttgcttctg gagagaaggc tggcataccg gcaatcgaat tggtcatggt aataaggagt
    12241 aaccgcgtgc gtaaagaccg tacattatta tcgtaagcaa gagtgataga atatgatcga
    12301 ttacgcttta atatacggta ggcatcacct aagaaagcaa aagggcctaa tttacgcttc
    12361 atttcagaag tgacattagc agcaatatca gcaaggagac caagtgttaa actgctaatc
    12421 atgtaatcgt cattaacttt acagatatct acggacttta catgaccgtt tagaaggttt
    12481 tcaagagcga gattcctctc ttgcgggatg ttcaaagctt ttgaaaagtt attgacggtc
    12541 ccggcaggta ctagtccgat agtaggataa gcaccaccag cgtagacacc accacagatt
    12601 ttattaatag tgccatctcc ccctaaagga ataaccaagt cgatatggtc ttcagatgct
    12661 ttttttgcta aggcgacagc ttcctcaaca gatgaaggtg taataatacg aacataatcg
    12721 tcttgaaaat catgttctat taaataagat tttacttctt ttgccaatgc ttgttcatct
    12781 ttaccagatt ttggattaaa aaagatataa gcacttttca tatgtccac
//
LOCUS       DATROY010000051        12734 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.052, whole genome shotgun sequence.
ACCESSION   DATROY010000051 DATROY010000000
VERSION     DATROY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            3..2567
                     /locus_tag="VBD11_001112"
     CDS             3..2567
                     /locus_tag="VBD11_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6954072.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            2690..3208
                     /locus_tag="VBD11_001113"
     CDS             2690..3208
                     /locus_tag="VBD11_001113"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6954073.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            3326..4006
                     /locus_tag="VBD11_001114"
     CDS             3326..4006
                     /locus_tag="VBD11_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6954074.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            4110..4793
                     /locus_tag="VBD11_001115"
     CDS             4110..4793
                     /locus_tag="VBD11_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6954075.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            4783..5571
                     /locus_tag="VBD11_001116"
     CDS             4783..5571
                     /locus_tag="VBD11_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6954076.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            5580..6683
                     /locus_tag="VBD11_001117"
     CDS             5580..6683
                     /locus_tag="VBD11_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6954077.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            6742..7611
                     /gene="rfbA"
                     /locus_tag="VBD11_001118"
     CDS             6742..7611
                     /gene="rfbA"
                     /locus_tag="VBD11_001118"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6954078.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7611..8204
                     /locus_tag="VBD11_001119"
     CDS             7611..8204
                     /locus_tag="VBD11_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6954079.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            8411..9457
                     /gene="rfbB"
                     /locus_tag="VBD11_001120"
     CDS             8411..9457
                     /gene="rfbB"
                     /locus_tag="VBD11_001120"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6954080.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(9509..12727)
                     /gene="hylB"
                     /locus_tag="VBD11_001121"
     CDS             complement(9509..12727)
                     /gene="hylB"
                     /locus_tag="VBD11_001121"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6954081.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKAKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
BASE COUNT         4074 a         2048 c         2587 g         4025 t
ORIGIN      
        1 ctatgagacc tatcgttaga aaaaagatgt acaaaaaagg taaatttttg gtcattgcag
       61 gtatcgttac tattttaggt ggtagtgcaa ttcttggtca agatgtcaag gcagaacagg
      121 cagaagcagt aacttcaacg atctcagaga aaactgatag cagtcagact atttcggata
      181 tttccaagtt aacgttacct gttaattcct cggaggctat gaaaaatagt gcagagcctt
      241 taataaaaac aggctttgct acatctgttt catctaatcc tagagagata gcagctaccc
      301 cagtcaaaac ctttgatgct agttcaaaag ttgttgtaaa agctagtact gctgagcact
      361 cagcaaatca aacaaattct aatgttaatc aagtagctaa tgatagtgaa gttattactc
      421 aacaaaattc aacaaagcaa ttaccaacag tgacttactc tgctcatgtt caagatattg
      481 gatggcaaaa gtcggttgat aatgcaacag tttcaggaac agtaggacag gaaaagcagg
      541 ttgaggcaat aaaattgtct attaaagccc ctgaaggaat aacaggaaaa cttagctata
      601 aaacttatgt taaaggacaa ggttggcaac cttcagtaga gagtggtcaa gttagtggta
      661 ctgttggtca gagtagacct attgaagcac taagcattaa ccttactgat aatttacaaa
      721 aattatatga tgtctattat cgtgttcatg ttcaagatat cgggtggatg gcttgggcta
      781 aaaatggagc ttacgctggg actttgggaa tgtcaaagcg ccttgaagct tacaaagtaa
      841 agtttaccct taaaggacaa tctgttttaa caccaactat acttaaggaa gagagacctg
      901 tcttaaatta tcaggttaag gtaggccaaa atggttggca atctaataaa cttgaaggac
      961 aaatggcggg aactttagga gaatccaaag ccctagatgg agtaaaattc actcttagca
     1021 ccttgaagta tggggacatt ctctatcgta ctcatgtaca agataaaggt tggggagaaa
     1081 ttttaggtag tcaaacaagt aaggattatc aaggaaaaag actagaagct attcaattgg
     1141 acttaagtgg gaatttaaaa caaaattatg atatttatta tcgcgctcac gtccaagata
     1201 aaggttggat ggcttggcaa aaaaataata gtgtagcagg tacagtcgga gaatcgaaac
     1261 gtttagaggc tttggaagta aaactagtag ctaaagcttc agattttagt tcccaaacaa
     1321 atcattcatt cttagaaatg aagcatcctg gattaagtta tcaaacttat cttcaaaaag
     1381 atggttggaa accaacggtt ttggaaggcc aattaggagg tagtattgga ctttcaaaat
     1441 ctataaaagc catcaaactt aacttaggta gtacagctct aggaaatata gaatatagaa
     1501 cttttcttaa tggttcaggt tggcaaactg ttgttaattc agggagagaa agtaatgtac
     1561 caaatgaatc tcaacaagta gaagctattc agatgcgatt gacaggtctg ctatcgaaaa
     1621 cctatagtgt gatgtatcga gttcacatgc aagattatgg ttggcaggat tggacttata
     1681 acaatgacat cgctggtagt actaaccaag gaaaacgaat tgaagcaatc gaaattaaat
     1741 tagttgagac tgctaaaatg cctataactg tccaaacgac ctataaggga actggaaact
     1801 ataatgtacg agtgacaaat gttattagtc ctgggaattt aaaaattgct atttggtcag
     1861 ataaaaacaa tcaggatgac cttaagtggt ataccgtcac acccaaaaat catgaagcta
     1921 gattgacatt taatgttaca aatcatagag acaatggtaa atactttatc catgtttatc
     1981 aggaaaatgc ttctaatcaa aaacaattat tggtaaaaac ttctctacaa gtcgcacatt
     2041 caaattataa tactccttac tttagccaac gtgatggaag gtgggcaagt cgaagatatg
     2101 gtatggcaac tttaggagaa actggttgtg taccaacttc tctagctatg attttgtctt
     2161 ctttgaaagg agaaactgtt ttaccaacgc aaattgcaga ttacctttac cataagacag
     2221 ttgaatttaa tcgtggtgtt caaggaacaa cttctagagg aatcttaaaa gcagccaaag
     2281 aatggggtgt aacagcaact gcattaggaa ctcaagctaa ccttgtacaa gcgttgaagg
     2341 atggttatca tgtcctagcg gcagtacaaa ataatgtttt tgttcttcat ggaagtcatg
     2401 aaatcgttct caaaggatat aataacggac tgacacatgt ctctgaccct tacacaccgt
     2461 ccttgtctgg ttggtatcca atttcacaac tttggaaaga acagagctat tatagtgagg
     2521 atagaattga tattggagca ccttttgtca aggtaacaga tgcctaattt ttaaaaccat
     2581 atgcaaaact agcatatggt tttaaagttt ccagtactat tttatttaat tttatgatat
     2641 aatgaagagt aaaacatttt aggggtttcc ccttagaaag acaagtgaaa tggatttaaa
     2701 taattacatc gcttcaattg aaaactatcc tcaagaagga attactttcc gtgatatctc
     2761 cccattaatg gctgatggta aagcatacag ctatgctgtt cgtgaaattg tacaatatgc
     2821 tgctgataaa gatattgata tgattgttgg accagaagct cgtggcttta ttgttggttg
     2881 cccagttgct tatgctcttg gtattggttt tgctccagtc cgtaaaccag gtaaattacc
     2941 acgcgaagtt atttcagctg actatgaaaa agaatatggt cttgatacat tgacaatgca
     3001 cgcagatgct attaaaccag gtcaacgtgt tttaattgtt gatgaccttt tagctactgg
     3061 tggaacagta aaagcgacaa ttgaaatgat tgaaaaactt ggtggcgttg tcgcaggctg
     3121 tgctttccta gttgaacttg atggattaaa tggtcgtaaa gctattgaag gttatgatac
     3181 taaggtattg atgaactttc caggataagg cctattcaag atagatgaat gataagggcg
     3241 cctctaagtg ctatgataat agttgagagg tgctttttgt tacacatttc ataattttag
     3301 gacgatagag aggatttgaa aggttatgac ttatttggaa caatatcagt cagggcaatt
     3361 gactctacca agtgctttat tttttcactt taaatctatt tttaagacag cagacgattt
     3421 tttagtttgg caatttttct atttacaaaa tacaactaat ttatccgatc taacaccgag
     3481 tcgtattgcg acatcacttg ataaaacagt ggcagatatc aatcgttcta tatcgaattt
     3541 aacaagtcaa ggtttactag atgtcaaaac aattgagttg aatcacgaaa tcgagattat
     3601 ctttgataca agccctgtgt ttgctaaatt agataaactt ttcgaagagg ataatcaagt
     3661 aataatagac aataaaaccg ctgattcgaa tcgtctgaaa gatttagtag gagacttcga
     3721 acgtgaatta ggtcgcttat taagtccttt tgaattagaa gatttacaaa agacactgca
     3781 agaagatcag acagatcctg atattgttag ggcagctctt agagaagcgg ttttcaatgg
     3841 gaaaacaagt tggaattata tcaatgctat ccttcgcaac tggcggcgtg aaggattgac
     3901 gacacttcga caaattgaag agagaaaaca agcacgtgaa gataaccaaa tgaaagattt
     3961 agcgatttcg gatgatttta aaaatgcaat gaatttatgg gaataagttt actaggagga
     4021 gacagaatgg ttgatacatt aggtctgctc cttttctttt gatgttaatg atgttatact
     4081 tttaacagag aaaaactgaa ggaaatacaa tggatttaca attatcaaaa cgattacaaa
     4141 aagttgcaaa ctatgttcca aaaggagctc ggttattaga tgtaggaagt gatcatgctt
     4201 acttacctat ttttttgttg cagatgggat actgtgattt tgccattgct ggtgaagtgg
     4261 tgaatggacc gtatcagtct gcgttgaaaa acgtttcaga acatggtctc acttcaaaga
     4321 tagatgttcg cttagcaaat ggtttgtcag cttttgaaga agcagataat attgatacga
     4381 tagctatctg tggaatgggt ggtcgtttga ttgctgatat tttgaataac gatatagaca
     4441 aacttcagca cgttaaaaca ctagtcttac aacctaataa tcgggaagat gatttaagaa
     4501 aatggttagc tgcaaatgat tttgaaatag tagcagaaga tatcttgact gaaaatgaca
     4561 aatgctatga aattttagtt gttaagcatg gtcatatgaa tttaactgct aaagaattgc
     4621 gatttggtcc atttttatta tccaataaca caacagtttt taaagaaaaa tggcaaaatg
     4681 agcttaataa attaactttt gctttaaact ctattcccaa tagtaaaatg gaagaacgtg
     4741 ctattttgga agataaaata caggatataa aggaggttct tgatgaaagc tagagaactt
     4801 attgatgtat atgaaactta ttgtccacag gaattatcta tggaaggaga tatttcaggt
     4861 cttcaaattg gatctttaga taaagaaata aagacagtca tggtagcttt agatgttcga
     4921 gagactacag tagctgaagc aattgaacgt caagtagatc ttcttattgt aaagcatgct
     4981 cctatttttc gtccattaaa ggatttggta gcaacaccgc aaaataagat ttatatagac
     5041 cttcttaaat cagacattgc tgtttatgtg agccatacta atatagatat cgttcctaat
     5101 ggacttaacg actggttttg tgaattatta gatattcaat atcccgatat attatctgag
     5161 acaagtaatg gttatggtat tggtcgcatt ggtgatgtta gaccccagag ttttgaattt
     5221 tttgcatgga aaattaaaga tgtgtttggt ttagatagcg ttcgtttagt ttcatatgat
     5281 aaatctaatc ctgaaattca acgagttgct atttgtgggg gtagtgggca aagtttctac
     5341 aaagaagcta ttgcaaaagg agcagatgtt tttgtaacgg gagatattta ttatcatact
     5401 gcacaagaaa tgatcacaaa tggtttgctt gcgattgacc caggccatca tattgaggtt
     5461 ttatttgttt ctaaaatagc tactatgatt gagcagtgga aacttgaaaa aggctgggat
     5521 atttcagttt tagagagtaa agctccaaca aatccctttt accatatgta ggagctatta
     5581 tgaaaaagat agcaattatt ggtgcaggag cagtcggagc gacactagcc tattacttat
     5641 caaaagaaaa ggatattcag gttactgttt ttgattatgg agttggacaa gcaactaagg
     5701 ctgctgcggg gattattagc ccatggtttt caaaacgtcg taataaagcc tggtatcgca
     5761 tggcgaggtt gggggctgat ttttattcaa agctagtcac ggatttacaa aaagatggtt
     5821 ttgaaacaaa attttatcag caaactggtg ttttcttatt aaaaaaggac gagagtcagt
     5881 tagagtcatt gtttgcacta gcagataaac gtcgactaga atctcctcta ataggtgatt
     5941 tacaaatact aaataaaagt gaagcaaata ctcactttcc tgagttagat ggctatgaac
     6001 agttacttta tgcgagtgga ggagcgcgag tagaaggggc ggacttaaca cgaatattgt
     6061 tagaagcaag tggtgttaat gtcatcaagg atgaagttca ctttacgata actgataatg
     6121 gctttcgtgt tcaaggaatt gattttgata aacttgtttt agcgagtgga gcctggctag
     6181 ctaagatatt agatgagcac aactatcagg tagatattcg ccctcaaaaa gggcaattgc
     6241 gagactatta ttttagcaat attaatacag gaaaatatcc tgttgtaatg ccagaaggcg
     6301 agctagatat tatcccattt gataatggta aagtctctgt aggtgcaagt cacgaaaatg
     6361 atatggcttt tgatttaaac attgatttta aagtcttaga caagtttgaa gagcaagcta
     6421 ttggctattt tccgcaatta aaaaaagcag atacgacttc tgaacgtgtt ggaattcgtg
     6481 cctatacaag tgatttttct ccattttttg gccctgtccc ttgtatggag ggtgcatatg
     6541 ctgcaagtgg attaggatca actggcttaa cagtgggccc attaattggt tatgaattat
     6601 gtcaattaat cttaaataaa gaaaatcagc taaatttaga ggattacgat attacgaaat
     6661 acgtgaaaaa gattagtaga tgatagcgtt taaaaaaagt gttataatat gtaggattaa
     6721 tataaataag gagaccttac tatgaaaggt attattttag cgggaggttc aggtactcgc
     6781 ttgtacccat taactcgtgc tgcatcaaaa caattgatgc caatttatga taaaccaatg
     6841 atttattatc cattgtcagt tttgatgttg gcaggaataa aagaaatact gattatttct
     6901 actccacaag atttgcctcg ctttgaagat atgcttggag atggttcaga actaggaatc
     6961 tctctttctt atgctgaaca accaagccca gatgggttag cacaggcctt cattatcggt
     7021 gaagaattta ttggcgatga tcacgttgcc ttagtattag gtgataatat ttaccatgga
     7081 ccaggtttat ctgcaatgct acaacgtgca gctagtaaag aaagtggagc aaccgtcttt
     7141 ggctatcaag taaaagatcc agaacgtttt ggtgttgtcg agtttgatac tgatatgaat
     7201 gccatttcta ttgaagaaaa accagcgcaa cctaagtcta attatgctgt cacaggtctt
     7261 tatttctatg ataacgatgt ggttgaaata gctaaaaata ttaagccaag tcctcgtgga
     7321 gaacttgaaa ttactgatgt taacaaagcc taccttgata gaggtgattt atcagttgag
     7381 ttaatgggac gtggatttgc gtggttagac actggaactc acgaaagtct tcttgaagca
     7441 gcacaatata ttgaaacagt acaacgcatg caaaacgttc aagttgctaa ccttgaggaa
     7501 attgcttacc gcatgggcta catcactcgt gaacaagtcc ttgagttggc acaaccactt
     7561 aagaaaaatg agtatggtca atacctactt cgtttgattg gagaagccta atgactgaac
     7621 aattttttga taaagaatta acttgtcgcc caattgaagc cattcctgga ttgttggaat
     7681 tcgatattac cgttcgtgga gacaaccgtg gatggttcaa agagaacttt caaaaagaaa
     7741 aaatgatacc gcttggtttc ccagaaagct tctttgaggc agacaaacta caaaataata
     7801 tttcgtttaa caaaaaaaat actttgcgag gtctccatgc agagccttgg gataaatatg
     7861 tttcgatcgc tgatgaagga cgtgtgatcg gtacttgggt tgacctccgt gaaggtgaca
     7921 gttttggtaa cgtttaccaa acgattatcg atgcctcaaa aggtattttt gttccacgcg
     7981 gcgttgctaa tggtttccaa gttctttcag ataaagcagc ttatacttat ctcgttaacg
     8041 attattgggc acttgaactc aaaccaaaat atgctttcgt taactatgca gatccaaatc
     8101 taggcattca gtgggaaaac cttgaagaag cagaagtctc agaagcagat aagaatcacc
     8161 cacttctcaa agatgtaaaa cctttgaaga aggaagattt gtaattttca acttcttaaa
     8221 aggcattaac cttagcaagt aaaaacgaat tagcaatgtt gtttgacgca agggtgaaag
     8281 gaaacatgca aaaggcctgt tccctcaggg atttgaaata ttgatttctt aggcatgaca
     8341 ttccatagaa ctacttctaa cagaacagta tccttcaaac agcaaaaaga aaaaattttt
     8401 cggagaaaaa atgactgaat ttaaaaacat tatcgtaact ggtggagctg gtttcatcgg
     8461 atcaaacttt gtacactatg tttacaataa ccacccagat gttcatgtaa ctgtccttga
     8521 taaattgaca tatgcaggta accgtgctaa cattgaagcc attcttggtg atcgtgttga
     8581 gttagttgtt ggtgacatcg ctgacgctga attggtagac aaattggctg ccaaagcgga
     8641 tgctattgtt cactatgcgg ctgagagtca caacgataac tcattggaag atccaagtcc
     8701 atttatccat acaaacttta tcggaactta cactttgctt gaagcagctc gtaaatacga
     8761 tatccgtttc caccacgtgt caactgatga agtttatgga gatcttccac ttcgtgaaga
     8821 ccttccagga aatggtgaag gaccaggtga aaaattcact gctgaaacaa aatacaatcc
     8881 atcatcacct tactcatcaa ctaaggcagc ttctgacctt atcgttaagg catgggtacg
     8941 ttccttcggc gtaaaagcga ccatttcgaa ctgttcaaat aactatggac cataccaaca
     9001 catcgaaaaa ttcattccac gtcaaattac aaatatcttg gcaggaatca aaccaaaact
     9061 ttatggtgaa ggtaaaaacg tccgtgactg gatccacaca aatgaccatt caacaggtgt
     9121 ctgggctatt ttgactaagg gtcgtattgg tgaaacatac cttattggtg ccgacggtga
     9181 gaaaaacaac aaggaggttc ttgaacttat ccttgagaaa atgggtcaac caaaagacgc
     9241 ttacgatcac gtaactgacc gtgctggtca tgatcttcgt tacgctatcg attctacaaa
     9301 attgcgtgaa gaactcggct gggaaccaca atttacaaac ttttcagaag gtttggaaga
     9361 aactattaaa tggtatacag aaaatgagac atggtggaaa gcagaaaaag atgctgtaga
     9421 agccaagtat gctaaaactc aagaagtcat taagtaattg aaccaaaaaa cctatcattt
     9481 tgatgatagg ttttttatgt acggctagtt agatagctaa ttggtctgtt tttgtcatgg
     9541 tttgaggttg gtaatagaca ttttgatagt cattaccaac tttttgtact aggtaaagtc
     9601 ccgctttatt cattttgaat tgattgttaa tgagactctc ttgattatcg tgtttgataa
     9661 cagcccaagt ttgactgttt ttatcataga taacttgttg ttttgaacta ttttctagta
     9721 attctacttc tttgctgttg gctaatttat caaaacttgt gcgatcaatg tttggaacca
     9781 tcatgtaacc atagctatca cctttgttgt catgcttttg gcttatagtg ataaaaggat
     9841 tgctaacgat tgaggtattt ttagaagtac gattaatgct gttccaagta cctgtttgct
     9901 ctttgcgttc aatatcaata gtgctatttt taaagaagat ataaccaata ttgcgaccag
     9961 gttcttttga ttctaaaaag acactttttg tatctgtaaa ttgttgagaa cttgcttgtt
    10021 ttaaatcaat agtttttcca ttgacgtatg tagtataagg tgttttagaa tcgtcttttc
    10081 gttgatcaat tgttgtagaa acatttccaa tgccgttagt attcttgatg ttgctaccta
    10141 aaaagacaat cttatcattt aagataaccc aacctttttg tgctgttaag gtgcgatccc
    10201 agttagtaaa atccatagcg gcaagagcaa agtgatcatt aagtttgacg gaaccaacaa
    10261 agtcagatgt tcctgtaact tgaccggttt tttctttagc gtctttgcta tgtttgctca
    10321 tgaattcctt agtggtatct tcacgcttag catctttttc agttgttcca gccattttat
    10381 aaggattgac ggttggccaa aaatgattac tataatgaga ttgatcacta ttataaatat
    10441 agaacatacc atctccggta taccaaccac gtgtattttc atcattcatt ccttcatagt
    10501 tgagggtacg tttagaatgt aatgaaagcg cgaaaccaaa gtctttcttg gcattataat
    10561 aagctaagcg gtccatgcta ttaaaggttg ataaattact ttttaaaggt ttagtagcga
    10621 ctgtactgtc attaagcagc ttattcatgt tggcaatatc ggaatacgat ttgaggttat
    10681 tgaagacatt gtagaattta tttgaagtga taatcgtttt aatagttgat ttgaggtcta
    10741 agtttcgctc ttcattagac atgttagcca atctgaggaa acctctgaga acttcaactg
    10801 cagccgcatg cgaagaagct gcctctctac taattgagcg tccacgactc atatccatta
    10861 actcaccttt tacaattaaa ggtaaaaatg attgattaat ccatttataa accatatcaa
    10921 gttcttgatt agagatttta tagtcagttt cttgaatgat aggcagcaat tgtgtcaaac
    10981 catctatcag aacattacca taggcgccag tataagcaac atttgtatgg tcgatgtaag
    11041 aaccgtcagc atagaagcct tcagctttag tagcagtagt aaaaagattt tttagagaat
    11101 gagaagtttt ttcgataata gtattgtctt tacgaagtaa accttcaatg attttaacgc
    11161 gccccatatc tactagatta ccaccaaggg ctttaaatgg attgacaagc gttttacgga
    11221 aatattctgc atcaggaaca aagtgttcaa ttgggtcggt ataagttttt atttcagcgt
    11281 cagtgaagta gttattcatg agagataggg tacctgtaat tgagcgagga acaccgattt
    11341 caaaatccca ccaattggca gagccttcta tatctttatt aacattgtag aagttttgat
    11401 gaagccaagc cagactctct tttacagtac gaatagcttt ttcatttttg taaatagtag
    11461 agtgtggatt ggtgatttgt ttagctaaat cttccaaacg acgataagta gcggttaact
    11521 gtgctgaatt attgagatta tctaaatctt tccaaaggaa agtgcgatta gaatccagtt
    11581 tgatagcttc gatgtttttg gcgttagttt catctaattt ctgattaagc ttttgcatgt
    11641 tcgaatcatt agtatcgtaa acataattac caatagtcac gtcgttccat ttgtcgagga
    11701 gttttgtaaa gttatcttct gttgaagctg taactgataa cggtacttct ttaattattt
    11761 tgccagattt atcagatatt ttaacagttg ttgaaccagt agcgtttgga tacaatattc
    11821 caccaacaat tttcccaaga gacggattgg ttagagtgta ttggtagtca gctttattaa
    11881 aaacgtaatt tttgtttaaa gccgtattaa cgcttttttc aatttgtgtt gtgacgggtt
    11941 gtggatgctc tgagtcttta gggccttttg ctttcattga tatattatca aaagtaacaa
    12001 aaccagttcc tttttcataa taaagttcaa gtgtcacctc ggagacattt aactttgggt
    12061 tatatatctt tgttaaggta tgtttgttag tagtaccgct ggtcatctca gaaagccaaa
    12121 gtcgcgtatt gttgtctttt ttctccataa tacgaaggaa agcttgtcca gccttgttac
    12181 ttgtttcaat atcaaactta acctcatatt ttttagtggg atcaatgttc actttttgtg
    12241 ttacagcacc tctaaagcct ttgtcgctgg atagacttac ttggccttgt tcggtacgtt
    12301 ggataatagg tgatttatca gcagtatttc ctttatcaat ccaagcagac cattccctag
    12361 cgcttgtatg tgaccagctt ccagatacag ggttagtttg attaaaatct ccgtttttca
    12421 ccagctcctt attttgagtt tgattatcta ctacttgttt cattgtgcta tgagtggctg
    12481 ttgcagttgc agttgaagct gtggggttag acagatcttg ttgtgtggtt gtggtgctag
    12541 tctccttgac agtggtactt tcagtctgga gaacttcaat attattagtt tgagtatctc
    12601 ttatttggga cgttgaggta gttttggtaa gctcttcagc tttagcttgg taactattgt
    12661 taaccaatat tgttcctaaa ataagggctg aataaagcat aatacgatgt ttctttttga
    12721 tttccatttt tact
//
LOCUS       DATROY010000052        12486 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.053, whole genome shotgun sequence.
ACCESSION   DATROY010000052 DATROY010000000
VERSION     DATROY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12486
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1440
                     /locus_tag="VBD11_001122"
     CDS             <1..1440
                     /locus_tag="VBD11_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEO6954082.1"
                     /translation="KAEQRQANLYALALRANQFEKTGFKGLSRFIRMIDKVLENENDL
                     ADVEVALPQNAVNLMTIHKSKGLEFKYVFILNIDKKFSMVDITSPLILSRNQGIGIKY
                     VADMRHELEEEILPAVKVSMETLPYQLNKRELRLATLSEQMRLLYVAMTRAEKKLYLV
                     GKASQTKWADHYDLVSENNYLPLASRETFVTFQDWLLAVHETYKKQELFYDINFVSLE
                     ELTDHHIGMVNPSLPFNPDNKAENRQSEDIVHAISVLESVEQINQTYKAAIELPTVRT
                     PSQVKKFYEPILDIEGVDVMETITKTSVDFKLPDFSTSKKQDPAALGSAVHELMQRIE
                     MSSHVKMEDIQKALTEVNAETSIKAAIQIEKINYFFQENSLGKYIQEEVEHLHREAPF
                     AMLKEDPESGEKFVVRGIIDGYLLLENRIILFDYKTDKFVNPLELKERYQGQMALYAE
                     ALKKSYEIERIDKYLILLGGKQLEVVKMD"
     gene            1453..2370
                     /locus_tag="VBD11_001123"
     CDS             1453..2370
                     /locus_tag="VBD11_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6954083.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(2402..3778)
                     /gene="mnmE"
                     /locus_tag="VBD11_001124"
     CDS             complement(2402..3778)
                     /gene="mnmE"
                     /locus_tag="VBD11_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6954084.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARHG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            3906..5816
                     /locus_tag="VBD11_001125"
     CDS             3906..5816
                     /locus_tag="VBD11_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6954085.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            5966..6934
                     /locus_tag="VBD11_001126"
     CDS             5966..6934
                     /locus_tag="VBD11_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6954086.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            7009..8007
                     /locus_tag="VBD11_001127"
     CDS             7009..8007
                     /locus_tag="VBD11_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6954087.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            8134..9522
                     /locus_tag="VBD11_001128"
     CDS             8134..9522
                     /locus_tag="VBD11_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6954088.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            9582..11339
                     /gene="lpdA"
                     /locus_tag="VBD11_001129"
     CDS             9582..11339
                     /gene="lpdA"
                     /locus_tag="VBD11_001129"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6954089.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            11437..12426
                     /locus_tag="VBD11_001130"
     CDS             11437..12426
                     /locus_tag="VBD11_001130"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6954090.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT         4112 a         2021 c         2543 g         3810 t
ORIGIN      
        1 aaagctgagc aacgtcaagc caatctatat gccttggcac ttcgagcaaa tcagtttgaa
       61 aaaacaggtt tcaagggttt atcacgtttt attcgaatga ttgataaagt tttagaaaat
      121 gaaaatgatt tggcagatgt tgaggtggca ttaccgcaaa atgctgttaa cctaatgact
      181 atacacaaat caaagggtct tgagtttaag tatgtattta ttttaaatat tgataagaaa
      241 ttttcaatgg tagacataac ttcccctctt attttaagcc gaaatcaagg aattggtatt
      301 aaatatgttg ctgatatgcg acatgaactc gaagaagaga tactgcctgc agtaaaggtt
      361 agcatggaaa cacttcctta ccaacttaat aaaagagagt tacgcttagc tactttatca
      421 gagcaaatgc gtttacttta cgtagctatg acacgtgcag agaaaaaatt atatctggtt
      481 ggtaaggcta gtcaaaccaa atgggctgat cattatgact tagtatctga aaataattat
      541 ctcccattag ctagtcgtga gacttttgtg acctttcaag attggttact ggctgttcat
      601 gaaacctata agaaacaaga gttgttttat gacatcaact tcgtatcatt agaggaatta
      661 acagatcacc acatagggat ggttaatcct agtctacctt tcaatcccga taataaggca
      721 gagaatcgtc agtctgaaga tatcgtgcac gcgattagcg tgttagaatc tgttgaacag
      781 attaatcaaa cttataaagc ggctattgag ttaccgacag taagaacacc aagtcaagtc
      841 aaaaaattct atgagcctat tctagatata gaaggcgttg atgtgatgga aactattact
      901 aaaacatcag tagattttaa gttacctgat ttttctacaa gtaaaaagca agacccagca
      961 gctttaggat ctgctgtaca tgaattaatg caacgcatag agatgtcatc tcatgtcaaa
     1021 atggaagata ttcaaaaagc cttgacagaa gtgaatgctg aaacaagtat caaagcggct
     1081 attcagattg aaaaaatcaa ctattttttc caagaaaact cactcggtaa atatatacaa
     1141 gaagaagtag aacatctaca tcgagaggca ccttttgcca tgttaaaaga agatccggag
     1201 agtggtgaga aattcgttgt acgtggtatt attgatgggt atctactatt agaaaatcgg
     1261 ataattctat ttgattataa gactgataaa tttgtcaatc ctctggaatt aaaagaacgc
     1321 tatcaggggc aaatggcttt atatgcagaa gctttaaaaa aatcttacga gatagaaagg
     1381 atagacaaat acttaattct tttaggtggt aaacagctag aagtggtaaa aatggattaa
     1441 agaaaggaaa aaatgatagt cgaacaaaaa tttggaaatg gttttacttg gattaacatt
     1501 gaagcagagc aattgcgaac agaaacatcg gaaattcaag caaaatattt ggatagtgag
     1561 attattacat atgctctgga tgattatgaa cgagccttta tggaatgttc tcatattaaa
     1621 ggaaaagaag tcttgacgat tatttttaat actattgatc taaagcagaa agaaagttat
     1681 tatgagaccg ttccgatgac cttttgctta tcacacgacc gtttgataac ggtgacacgg
     1741 tctcgaaata gttatatgct agaactattg caaaaatatc tagaccgtaa tccagatgtc
     1801 tctcctaaaa aatttttatt tgcagcttta actttgatta caaaacaata ttttaatgtt
     1861 gtatcgaaaa ttgatagaga aaaggatatt ctcaaccgtc aattgagaga acaaacaacc
     1921 aataagcgct tattagccat gagtgaccta gagacaggct ccgtttatct tttgacagct
     1981 gccaatcaaa atgcattggt attagagcaa ttagatgttc atcctagtca gcgatttaat
     2041 tctgaagtgg aaaaagaaca gttatcagat gctctcattg aagcgcatca gttagtcagt
     2101 atgacacaat taaacagtca ggtactttca caattatcat ctacttttaa taatgtctta
     2161 aataataact tgaacgaaaa cttaacaggt ttgaatatca tttcgattaa tttagcgatt
     2221 attgcagcaa ttactggctt ttttgggatg aatattccat tacctcttac agaatcacgg
     2281 agttcatggt taattgttat tgctacttcg gtcttacttt gggttattat tgctcaaatt
     2341 ttaaaacgag tttttaactt aagaaaataa aaatgttcag tgcgtaagca ccgaacattt
     2401 tttatttacc taagcagaat tggctgaaga gctgagttat tagctcatct ggagctgcat
     2461 ccccagtgat ttctccaagt atttcccaag ttctagtcat atcgacttgt aagagatcaa
     2521 caggcattcc taattccaag ccctcattga cagcttttaa actatcaacg gctttttcaa
     2581 ttagtgagat atggcgagca ttcgatagat aagtagcgtc atgttctacc ataccagcat
     2641 tgtcaaagaa aatatcgtta atacgttctt cgattttatc gatgttttca ttttctaaaa
     2701 ccgaaattgg aataacattt tcaggtaatt cgttaacttc tattttttga ggaagatccg
     2761 ttttattgag taatacgata cgattacttt ctttacttag ttctagaagg gagcggtctt
     2821 gtaatgttag tggttcggaa ctattgagaa ccaacaacac aaggtcggct tcttctaaag
     2881 ccttctttga acgttcaaca ccaatttttt caacgatatc atctgtatca cggataccag
     2941 cagtgtcaac tagttttaaa ggaactcctt taatattgac gtattcttca ataacatcac
     3001 gagttgttcc ttcaatatct gtcacaatcg ccttttcttc acgtaagagg ttatttagta
     3061 agctggattt accgacattt ggacgaccaa tgattgctgt tgaaagtcct tctcgaagaa
     3121 tttttccatg tcgggcagtt cgaagtaaat tttccataag tgcttgaaac tcttgtgttt
     3181 tttcacgcat cagtgttgta gtcatctctt caacgtcatc atattcaggg taatcgatat
     3241 taacttcaac ttgggctaga gtatttagaa tttcttgtcg agtgttattg attagtgtct
     3301 ttaaagaacc atctaattgc ttaacggcaa tatccattgc tttatctgtt ttagctctaa
     3361 taagatccat aacagcttca gcctgagtca agtcaactcg accgtttaaa aaagcacgtt
     3421 tagtaaattc tcctggttca gccattcgtg ctccatgtcg taaaattaac tgaagaattt
     3481 cgttagtaac tgcaattcct ccatgcgtat taatttcaat aacatcttct cttgtgaatg
     3541 tttttggagc aagcataacg cctaacataa cttcatccaa gatttcattt ttatctgggt
     3601 ccacaatatg accatagttt aaggtatggg attggatagc ggatagatct tttccacgat
     3661 aaatttttga tgctatcttt aaggcatctg taccagatat acggacaata ccaatagctc
     3721 cctcaccgag aggagttgaa atagctgcga tggtatcaaa ttcttttgta atagacataa
     3781 agtctctctc tttcttggga attactaaca ttcattgtaa cctaaagccc taaaaggtgc
     3841 aatcatttga ttaaaagaga agatttccat aatggtttta tttgaaggta tgctataata
     3901 aaactatgat tatactacaa ggaaacaaaa ttgagcgctc attttcaggt gatgtgcttt
     3961 ttgataatat taatattcaa gttgatcaac gtgatcgtat tgctcttgtt ggacgtaatg
     4021 gtgctggcaa atcaacgctt cttaaaattt tagttggtga agaggctccg acaaaaggag
     4081 aaataaataa aaagcgtgat ttgagtttgt cctacttggc acaagatagt cgttttcaat
     4141 ctgaaaatac aattttccaa gaaatgttac aagtttttga ttcattaaga gaagtagaga
     4201 agcgcttacg tgaattggaa ttacaaatgg gacaagtttc aggaagtgac ctggaacaat
     4261 tgatgaaaac atacgatatt ttgtcagaag agtttcgtga aaaaggtggc tttacctatg
     4321 aatcagatat aaaggctatt ttaaatggct ttaagtttaa cagtgatatg tgggaaatgc
     4381 ctattagtga attgtctggt gggcaaaata cacgattagc gcttgcaaaa atgctacttg
     4441 aaaagccaga attacttgtt ttagatgaac caacgaacca tttagacatt gatacgattg
     4501 cttggttaga aaattatctt gtaaattatc aaggagccct tattattgtc agccatgacc
     4561 gctatttttt agacaaggta gctacagtaa cttatgattt aacgacacat tctctagacc
     4621 gctatgtagg caattattcg aaatttatgg atcttaaggc agagaaaata gcgactgaag
     4681 aaaagaattt tgaaaagcaa caaaaagaaa ttgctaagtt agaagatttt gtacaacgta
     4741 acattgttag ggcttcaacc actaaacgag cacaagctcg tcgtaaacaa ttagaaaaga
     4801 tggaacgact cgacaaaccg aatgtagagc aaaaatcagc taatatgact tttcatgctg
     4861 ggaaagtctc agggaatgtt gttttaacac ttgagaatgc agctattggt tatgaaggtg
     4921 tttctttatc ggagccaatc gatttggacg tgaagaaatt tgatgctatt gcaattgtag
     4981 gtccaaatgg tatcgggaaa tcaactttaa tcaaatcgct tgttgggcaa attcctttta
     5041 tcaaaggaga agcaaaatta ggagcaaatg tcgaaactgg gtattacgat caaagtcaaa
     5101 gtaatttaac aaaaacgaat actgttttag atgagttatg gaatgctttt tcaacaactc
     5161 cagaagttga aattagaaac cgtttaggag cttttctttt ttcaggtgat gatgttaaaa
     5221 aatcagtttc aatgctatcc ggtggtgaac gagcgcgttt gctattggca aaattatcta
     5281 tggaaaataa taatttcctc atattagatg agccaacaaa ccacttggat attgatagta
     5341 aagaagtctt agaaaatgca ctcatcgaat ttgatggcac attacttttt gttagtcatg
     5401 atcgctattt tattaatcgt gtggcaacta aggttctcga aatttctgat aagggaagta
     5461 ctctctatct aggagattat gattattacc tcactaagaa agccgaacta gaggaattag
     5521 cccgtttaaa tgaagaagaa gtgagtgctt caaaaacaga aatcgacgtt acatcagatt
     5581 atgagacaca aaaagcaaat caaaaggaat ttaggaaaat tacccgtcgc gttgcagaaa
     5641 ttgaggcacg tttggaagtt cttgaaaatg atgaaaacaa tatcaatggt ttaatgttag
     5701 aaaccaatga catcggaaaa ctctctgatt tacaaaaaga gctggaatca atccaagaag
     5761 aacaattgct attaatggaa gaatgggaaa atctaaatat gagactagat ttgtgagaaa
     5821 atttcaaaaa caatagtttg tgaaaaaaag tacattttaa attgagatgc cttttacgaa
     5881 aacaaagaaa gcggttcaaa ttcttgcatc ttgtgaaaaa aacttataca atgattaagt
     5941 aataaagatt tttttggagg ttagaatggt aactctatct aaagaacaac acttggatat
     6001 gtttctaaaa atgcaacgta ttcgtgatgt tgatatgaaa tttaacaaat tggttcgtcg
     6061 tggtttcgtc caagggatga ctcacttctc tgttggtgaa gaagctgctt cagtaggtgc
     6121 tatccaagat ttaacagatt cagatatcat tttctcaaac caccgtggac acggacaaac
     6181 tattgcaaaa ggtattgata ttggtggaat gtttgctgaa cttgctggta aagcaacagg
     6241 aacatcaaaa ggtcgtggag gctcaatgca cttagcaaac cttgaaaaag gtaactatgg
     6301 aacaaacggt atcgtcggtg gaggatatgc gcttgcggta ggtgctgccc ttacacagca
     6361 atatgaagga actgataaca ttgttatcgc cttttcgggt gactcagcga ctaacgaagg
     6421 ctcattccat gagtcagtta acttagctgc tgtttggaac ttaccagtta tcttctttat
     6481 catcaacaac cgttatggta tctcaacaga catcacatac tcaactaaaa ttcctcacct
     6541 ttacatgcgt gctgatgcgt acggtattcc agggcattat gttgaagatg gtaatgatct
     6601 tatggctgtt tacgagaaaa tgcatgaagt tatcaattac gtgcgttcag gaaatggacc
     6661 agctattgta gaggttgaat cataccgttg gtttggtcat tcaactgctg atgctggggt
     6721 ataccgtact aaagaagaag ttgattcatg gaaagctaaa gatccagtaa aacgctaccg
     6781 tgcatactta attgaaaatg aaatcgctac tgaagaagag cttgcagcta ttgaagcaca
     6841 agtgattaaa gaagttgaag aaggggttaa attcgctgaa gaaagtccat tccctgatat
     6901 gtctgttgca tttgaagacg tttttgtgga ctaaaataac ctaaagtaga tgagatttat
     6961 ctcgttctat tattcaattg attaaaaaac ggatattcgg aggaaataat gagcgaaact
     7021 aaagttatgg ctttgcgcga agcgattaat gtcgctatga gcgaagaaat gcgcaaagat
     7081 gaaaaagtat ttttaatggg tgaagatgta ggtgtatatg gaggagactt cggtacatct
     7141 gttggtatgt tagaagagtt tggtgctaag cgcgttcgcg acacaccaat ctcagaggct
     7201 gcgattgcag gttcagctat cggtgctgca caaacaggtt tgcgtcctat cgttgatttg
     7261 acattcatgg atttcgtaac aatcgcaatg gatgctatcg ttaaccaagg tgctaaaacg
     7321 aattatatgt ttggtggagg tctatcaact ccagttactt tccgtgtggc atcagggtca
     7381 ggtattggtt cggctgcaca acactcacaa tcattagaag cttggttaac acacattcca
     7441 ggtctaaaag ttgttgcacc tggtactgtt aatgagtcaa aagcactttt gaaatcttct
     7501 attcttgaca ataacccagt tatcttctta gagccaaaag ctctctatgg taagaaagaa
     7561 gaagttaata tggatcctga tttctatatt ccacttggta aaggggatat taagcgtgaa
     7621 ggtactgact taacaattgt ttcttatggc cgtatgcttg agcgtgttat gcaagctgct
     7681 gaagaagttg ctgaggaagg tattaacgtt gaggttgttg atccacgtac acttattccg
     7741 cttgataaag aattaattat cgattctgtt aagaaaactg gtaaattgat cttggttaac
     7801 gatgcttata aaactggtgg tttcactggt gaaattgcaa ctatggttgc tgaaagtgaa
     7861 gcatttgatt accttgatca tcctatcgtg cgtcttgcaa gtgaagatgt tccagtaccg
     7921 tattcacgtg tacttgaaca aggaatctta cctgatgtag caaaaattaa agatgctatc
     7981 tacaaagtag ttaacaaagg taaataattc ataaaacaaa gagggactga ggaatgagtg
     8041 aaaacttagc ctctttcctt gtttataggt tttaatttat atcgttagat gaaacatata
     8101 tttgacgata tagtttagtg tgaaaggaaa actatggctg tcgaaattat tatgcctaaa
     8161 ctcggggttg acatgcaaga aggcgaaatc cttgagtgga aaaaacaggt aggtgatgtt
     8221 gttaatgaag gtgacgtcct acttgaaatt atgtcagata aaacaaatat ggagattgaa
     8281 gcagaagact caggtgtttt actaaaaatc acacatggga atggagatgt tgttcctgta
     8341 actgagacaa ttggctgtat cggtgctgaa ggtgaagaag taacagaagc atcttcttct
     8401 gaaaatacat cagttgaaga aaacgcgaca caagtaactt cagaaccaga aaaagttgaa
     8461 gaaacatcag aaccaagtgt gccagcagct acaagtggtg aaaaagtacg tgccactcct
     8521 gcagctcgta aattagcaag agagatgtct attgaccttg cactagtatc tggaacaggg
     8581 gcaaatggtc gcgttcacag agaagatgtt gaaagcttca agggtgctca accacgtatt
     8641 acaccattgg cacgtcgaat tgctgaagat caaggagttg atatagcaga aatcactggc
     8701 agtggtattc gtggtaaaat tgttaaaaat gatgttttgg ctgctatgtc accgcaagca
     8761 gcagaggctc ctgtaaaaac gaaagctaca ccaacaacag aagaaaaaca attaccagaa
     8821 ggtgttgaag tcattaagat gtctgcaatg cgtaaggcta tttctaaagg tatgactaac
     8881 tcttacctca ctgctccaag ctttacactt aactatgata ttgatatgac tgaaatgatg
     8941 gctcttcgca agaaattaat tgatccaatc atggctaaaa caggcttgaa agtcagcttt
     9001 actgatttga ttggaatggc cgttgttaag acattgatga aaccagagca tcgataccta
     9061 aatgcttcat taattaacga tgcacaagaa attgagcttc ataattttgt taatattgga
     9121 atcgcagttg gtcttgatga tggtttgatt gttcctgttg ttcacaatgc tgatcaaatg
     9181 tcactttctg actttgttat agcatctaaa gatgttatta agaaaactca agaaggtaaa
     9241 ttaaaatcag ctgaaatgtc aggttcaaca ttctcaatta ctaacttagg tatgtttggt
     9301 actaagacgt ttaacccaat tatcaatcaa ccgaactcag caatccttgg agtaggtgct
     9361 actattccga ctccaacagt ggttgatggt gaaattgtag ctcgaccaat catggcaatg
     9421 tgtttaacaa ttgaccaccg tattgttgat ggaatgaatg gtgctaagtt tatggttgat
     9481 cttaagaact taatggaaaa cccatttggt ttacttattt gattccgtag gtcttaattt
     9541 gatataataa gttattcgaa aaacgaaaga ggaaataaaa aatggctttt gatgtaatta
     9601 tgccaaaact tggggttgac atgcaagaag gcgaaatcct tgagtggaaa aaaaatgaag
     9661 gtgacaccgt taatgaaggt gatgtccttc ttgaaatcat gtctgataaa accaatatgg
     9721 aaattgaagc agaagacact ggtgtacttt taaaaattgt acatcaggca ggagatgttg
     9781 ttcctgtcac tgaagtgatt gcctatatcg gagaagaagg tgaagaagta ggtacgtcat
     9841 caccttcggc tgatgcaact atcacagctg aagatggtca atcagtttct ggacctgctg
     9901 ctccttcaca agagacagtt gcagcagcaa ctcctaaaga agaacttgca gctgatgagt
     9961 acgatatcgt agtcgtaggt ggtggccctg caggttatta cgcagcaatc cgcggtgctc
    10021 aacttggagg aaaaattgcc attgttgaga aaactgaatt tggcggtacg tgtttgaatg
    10081 ttggttgtat ccctacaaaa acatatctta aaaatgctga aatcctagat ggtttgaaag
    10141 tagcagctgg acgtggtatc aaccttgctt caactaacta tgcaattgat atggataaga
    10201 ctgttgcctt taaaaattct gtagttaaga cattgactgg tggtgtacgt ggtctcttga
    10261 aagcgaacaa agttgaaatt ttcaatggac ttggacaagt aaaccctgat aaatctgttg
    10321 ttattggcga taaagttatc aaaggtcgta acgtcgtcct tgcgactggt tctaaggtat
    10381 ctcgtattaa tattccaggt attgaatcac cattagtatt aacatcagat gatattcttg
    10441 atttacgtga aattcctaag tcacttgctg ttatgggtgg tggtgtcgta ggtattgagc
    10501 ttggtcttgt atgggcttca tatggtgttg acgtaactgt tattgaaatg gctgatcgca
    10561 tcattccagc aatggataaa gaagtatcgc tagaacttca aaaaatcctt gctaagaagg
    10621 gaatgaagat taaaacatca gttggtgttt ctgaaattgt tgaagccaat aatcaattaa
    10681 cgcttaaatt aaacaacggc gaagaagttg ttgctgataa agctcttctt tcaattggac
    10741 gtgttccaca aatgaatggt cttgaaaatc ttgagcctga acttgagatg gaacgtggac
    10801 gtattaaagt taatgcttat caagaaactt caattccagg tatctatgca ccaggtgatg
    10861 ttaatggaac tcgtatgtta gctcatgctg cataccgtat gggtgaagta gctgctgaaa
    10921 acgcactcgg cggtaataag cgtaaagcac atctagattt cactccagca gctgtttata
    10981 ctcatcctga agtagcaatg gtggggatga cagaagagca agcacgtgaa caatatggtg
    11041 acatattagt tggtaaaaat agctttacag gcaatggtcg tgctatcgct tcaaatgaag
    11101 cacatggttt tgtaaaagta attgctgaac ctaagtataa agagattctt ggtgttcaca
    11161 ttattggtcc agctgcagcg gaattgatta atgaagcttc tacgattatg gaaaatgagt
    11221 tgactgtcta tgatgtagca caatctatcc atggtcaccc aacattctct gaagttatgt
    11281 acgaagcttt cttagatgtt ctcggtgaag ctattcacaa ccctccaaaa cgcaaataat
    11341 tttgtttaat atatttgaga ttgagctttt atagcttcaa tctcttttca cgtatgtatt
    11401 gtctgaatat tataaaatat tagttaggaa tttaaaatga aatatatcgt taacacgtct
    11461 aatgatccgg cttacaatgt tgctttagaa gcttacgcct ttcaaaagtt gacagatatc
    11521 gatgaaatct ttattctttg gattaatgaa ccggctatca ttattggacg ccatcaaaat
    11581 acaattcaag aaattaacaa agagttcatt gataaaaatg gcatccatgt tgtccgtcgt
    11641 ttgtcaggag gcggagcagt ttatcatgac ttgaataatt taaactacac tattatttct
    11701 aataatacac aagaaggtgc ttttgatttc caaacttttt caaaaccagt tattgatact
    11761 cttgctaaat taggtgttaa agcagaattt actggtcgta atgatttaga aatcaatgga
    11821 caaaaatttg caggaaatgc tcaagcttat tacaaaggtc gtatgatgca tcatggttgt
    11881 cttttatttg atgttgatat gtcagtctta gggcaagcac tcaaagtatc taaagacaaa
    11941 attgaatcta aagggataaa atctgtacgc gcacgtgtta caaatattgt tgaccatctt
    12001 tcagataaaa ttacagtaca agaatttagc gatgccattt tagctcaagt gaaggaagaa
    12061 tatccagaga tggatgaata tgttttgagt gatgccgagt tatcagaaat ccaagctatg
    12121 cgcgataatc aatttgctac atgggattgg acgtatggta aagcacctga atacaccatt
    12181 gaacgtggtg tccgctatcc agctggaaaa attacgactt atgctaatgt tgaaaattca
    12241 acgataaaat ctgtcaaaat ctttggtgac ttctttggag tgaaacctgt agatgatatt
    12301 gaaaaaatgt tggaaggtgt ccgctacgat tacaaagatg ttttagcagc gcttaaaaca
    12361 gttgatacaa gtcaatactt ctcacgtatg acacccgaag agattacaaa agctattgta
    12421 gactaaaatc aaaaaaccta gtcaaagtct ttgcctttgg ctaggttttt tgtatggtta
    12481 agtggc
//
LOCUS       DATROY010000053        12380 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.054, whole genome shotgun sequence.
ACCESSION   DATROY010000053 DATROY010000000
VERSION     DATROY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12380
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            38..2299
                     /locus_tag="VBD11_001131"
     CDS             38..2299
                     /locus_tag="VBD11_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6954091.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            2485..2940
                     /locus_tag="VBD11_001132"
     CDS             2485..2940
                     /locus_tag="VBD11_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6954092.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            3004..3306
                     /locus_tag="VBD11_001133"
     CDS             3004..3306
                     /locus_tag="VBD11_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6954093.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(3376..6168)
                     /locus_tag="VBD11_001134"
     CDS             complement(3376..6168)
                     /locus_tag="VBD11_001134"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6954094.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(6453..7223)
                     /locus_tag="VBD11_001135"
     CDS             complement(6453..7223)
                     /locus_tag="VBD11_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6954095.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(7233..8021)
                     /locus_tag="VBD11_001136"
     CDS             complement(7233..8021)
                     /locus_tag="VBD11_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6954096.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(8023..8277)
                     /locus_tag="VBD11_001137"
     CDS             complement(8023..8277)
                     /locus_tag="VBD11_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6954097.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(8280..8885)
                     /locus_tag="VBD11_001138"
     CDS             complement(8280..8885)
                     /locus_tag="VBD11_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6954098.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(8897..9571)
                     /locus_tag="VBD11_001139"
     CDS             complement(8897..9571)
                     /locus_tag="VBD11_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6954099.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(9577..10857)
                     /gene="ftsZ"
                     /locus_tag="VBD11_001140"
     CDS             complement(9577..10857)
                     /gene="ftsZ"
                     /locus_tag="VBD11_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6954100.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(10879..12252)
                     /gene="ftsA"
                     /locus_tag="VBD11_001141"
     CDS             complement(10879..12252)
                     /gene="ftsA"
                     /locus_tag="VBD11_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6954101.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
BASE COUNT         3882 a         2473 c         2082 g         3943 t
ORIGIN      
        1 acatcaatag ttaaatgtca taagaaaggg gtagcctatg ctctgtcaaa actgcaaact
       61 aaacgaatca actattcatt tgtatacaaa tgttaatgga aaacaaaagc aagttgatct
      121 ttgtcaaaat tgctaccaaa ttattaaaac tgaccctaat aatccacttt tttcaggttt
      181 aaatcatgtt tcacatgcgc ctggtggcat caatcctttc tttgatgatt tctttggaga
      241 tttaaataat ttcagagcct ttaatggcca agatttacca aacactcctc ctacacaagc
      301 aggaggaaac cgaggtggag gaaacggtaa tggacgaaat aataatcgca accaaactgc
      361 aactcctcct caagccaaag ggattctaga agaatttggt atcaatgtca ctgaaatagc
      421 ccgtcatggt gatattgacc ctgttattgg acgtgactca gaaattattc gtgttatcga
      481 aatccttaat cgtcgtacaa aaaataatcc tgtacttatt ggcgaacccg gcgtagggaa
      541 aactgccgtt gttgaaggac ttgctcagaa aattgttgat ggtaatgtgc ctcataaact
      601 tcaaggcaaa caagttattc gtttggatgt tgtgagtcta gtacaaggaa cgggcattcg
      661 tggtcaattt gaagaacgca tgcaaaagtt gatggaagaa attcgtcaac gtcaagatgt
      721 tattctattc atagatgaaa ttcacgaaat agttggagct ggtacagctg gcgaaggtag
      781 tatggatgca ggtaatattt taaaacctgc ccttgcacgt ggagaattac aactagtagg
      841 tgccactact ctcaatgaat atcgtattat cgaaaaggac gctgccttag aacgccgtat
      901 gcaacctgta aaagtcgatg aaccttctgt tgaagaaacc attactattt tgaaaggtat
      961 ccaaaaaaaa tacgaagatt atcatcacgt aaaatataat aatgatgcca tagaagcagc
     1021 tgcagtacta tctaatcgtt atatccaaga ccgcttttta cctgataaag caatagactt
     1081 attagatgaa gctggttcta aaatgaacct aacactaaat tttgttgatc caaaagaaat
     1141 tgatcaacgt ctcattgaag cagaaaattt aaaagcgcaa gcgactcgtg aagaagatta
     1201 cgaacgtgca gcttacttcc gtgaccagat tgcaaaatat aaagaaatgc agcaacaaaa
     1261 ggtcgacgat caagatacac ctattattac cgaaaaaaca attgagcaca tcattgaaga
     1321 aaaaacgaat atccctgttg gtgatttaaa agaaaaagaa caatctcaat taattaatct
     1381 cgcagatgac ttgaaacagc atgtgatcgg ccaggatgac gctgtcatta agattgcaaa
     1441 agctattcgt cgtaatcgag ttggtcttgg tagcccaaac cgtcctattg gttccttttt
     1501 atttgtagga ccaaccggtg ttggtaaaac tgaactttct aaacaactag caattgagct
     1561 ctttggttca gctgatagta tgattcgttt tgatatgtca gagtacatgg aaaagcatgc
     1621 tgttgctaaa ttagtcggag cgcctccagg atacgtggga tacgaggaag ctggacaact
     1681 aactgaaaag gttcgtcgaa atccttactc gctcatcctt ctagatgaaa ttgaaaaagc
     1741 tcatcccgat gtcatgcata tgttcttgca ggtccttgat gacggtcgat taacagatgg
     1801 acaaggaaga actgttagtt ttaaagatac cattatcatc atgacctcaa atgctggttc
     1861 tggtaaaact gaagcaagtg ttggctttgg tgcctcacga gaaggtagga cgaattccgt
     1921 actaggtcaa ctaggtaact tcttcagccc tgaatttatg aaccgctttg acggtattat
     1981 tgaattcaag gctttagata aagagaatct cctcaatatc gttgatatta tgttatctga
     2041 cgttaatgca cgtctcgcca tcaatggtat tcatctagat gtcactgata aagtgaaaga
     2101 aaaattggtt gatttaggtt acgatcccaa aatgggagca cgtccattac gtcgtaccat
     2161 tcaagaacat attgaagatg ctatcacaga ttactatctt gagaatccaa gcgaaaaaga
     2221 acttcgtgct attatgacta gcaatggaaa tatcataata aaatcttcta aaaaaactga
     2281 ggaaagtaca aaaggttgag taagaaactc aacctttttt cacactcttc tcaaaaaata
     2341 atagcctcta taaagctgtt tctctcctat ctcgtcttta aattttaata caattattaa
     2401 ggaaatattc caaaaatttt agtataataa ataaaaaata attaaagctt ttaaaatcat
     2461 aaaacacaat caggagagag aactatgact aatcctactt tcggtgaaaa aatagataat
     2521 gtagactata ggtcacgatt tggtgtctat gctattattc caaatccaac tcatgataaa
     2581 attattttag tacaggcacc taatggtgct tggtttcttc caggtgggga aattgaagaa
     2641 aatgaaaatc acctagaagc tttaactcgt gagttaattg aagaattagg ttattctgca
     2701 acaattggcc attactatgg tcaagctgat gaatattttt actctagaca ccgtgatact
     2761 tactactata atcccgccta tatttatgaa gttactgcct atcataagga gcaagcgcca
     2821 ctagaagatt tcaaccatct agcctggttt cctatccaag aagctaaaga aaagctaaaa
     2881 cgaggaagcc atagatgggg tgtccaagct tgggaaaaaa atcaccattc taggaaataa
     2941 ttcatacctt tcttcctaaa tagtgttata ataaacctaa taagaatcag ggggaaaata
     3001 gttatgagac taattaatac aaccagtagt catcctgaac tagttcgaaa tcaactccaa
     3061 aatacagatg caaaattagt ggaagtctat tcagctggca acaccgatgt cgtttttaca
     3121 acagcaccta aacattatga attattaatt tcaaacaaat atcgtgctat caaagatgaa
     3181 gaattagaag ctattcgtga gttcttctta aagcgtaaaa tagatcaatc tattattatt
     3241 caagagcaga tgaaatcact ccatactgcc aagttaattg aaatttcata tccaacaaca
     3301 gcataattaa aagaagccta atcaaggctt cttttttgat gactactaaa caaatatagc
     3361 attaaataat ttttattaag cttcaaaacc ttgagctact gcttcaggat aatattgctc
     3421 aataattgct gcactagcat cacaaacagc tacaccatac gaacgcatcc ttgtggttgg
     3481 atcaatacgg cgtgagcgtt cgcagacttc accttcagcg tgttcaacag taaatgcaac
     3541 accttcaaat gatactgagt cagctggttt gtctgcttca tcagcaattg ttaattgtga
     3601 gacaatcatc aagagtgcaa tatcgctatt taacgctgtt aataacgttt tcacttcttg
     3661 actagcataa attgttaaat gtgcttctag agatttacca atgactttag cgttacgtgc
     3721 ttcttcgaga gctttttgag cttgtgttcg taacgtcatg aatgctgacc actcttcaag
     3781 aatttcttct tgaccagaga atgtctgtgc aacaggcatt tctgctaatt gaacaaattc
     3841 ctcctcctca tgttcaaggt atgaccaaat ttcttcagca gtatgtggta gaatcggagt
     3901 caaaagtttt gtaagtttaa ctaaaatatc gtagaaaaca gtttgcatac gacgtcgttc
     3961 tggactgtta gccgcttcaa tgtaaaccac atcttttgca aaatcaaggt aaaatgctga
     4021 tagatcaact gttacgaagt taacgacagc tttataaatt gccataaaat cataagcagc
     4081 ataagctttg ttgattgtat ccacaacttg attaaattta atagtcatat aacgatcaac
     4141 ggcacctaaa ttttcatagg ctactgcatc ttgtttagga ttaaaatcgg aagtatttgc
     4201 aatcaagaaa cgcaatgtat ttctgatctt acgataagtt tctgatgttt gttttaagat
     4261 atccattgaa atacgaacat cattacttga atcaacagaa gttacccaca gacgcaagat
     4321 ttccgcacca aattgttttt caacatcact cggaagaatt gtatttccta gagatttaga
     4381 cattttctca cctttaccat caagtacaaa accttgagat aagactgctt tgtatggagc
     4441 gtgaccatta acagcaactg atgtaattag tgatgagttg aaccaaccac ggtattgatc
     4501 agaaccttca agataaagat ctgctggata ggaaaggttt tcacgagcat tcattactcc
     4561 attccatgat gaaccagaat caaaccaaac atccatgatg tctgtttctt tctcaaagag
     4621 accattaggt gaacctggat gagtataacc tgctggtaga aggtccttag catcacgttg
     4681 ccaccaaacg attgagccgt attcagcaaa taaatctgca acgtgatcag tcacttcttt
     4741 tgtcatgata gctgtaccat cttctgcata aaaaattgga agtggaacac cccaagcacg
     4801 ttgacgtgag attacccagt cgccacggtc acggatcatg ttataaagac gttttttacc
     4861 ccactcaggt tggaaatttg tcttttcaat ttcatcgagg atttcttgac ggaatttcga
     4921 aacagaggca aaccattgtg gaaccgcacg ccaaatgatt ggctttttcg tacgccagtc
     4981 aaatggatat gagtggttga taacttcgct ggctaaaaga agatctccca atttttcttt
     5041 tacaagtggt gtcactttgt catagaattg accttcaaaa tcaggaccag cattttccat
     5101 catcaaacca cgactatcta cggtaactac aacatcaagt ccattagcaa taccaacgtt
     5161 atagtcatct tcaccaaaac caggagccgt gtggacaata cctgtaccag aatctgttgt
     5221 aacatggtct ccaaggataa ccaactcttc tacttctgta tcccatggat gttctgtaac
     5281 aatgtgatta agttctttac cagtgtgatg agtcacaatt tcaaagtttt cccagccaaa
     5341 cttagcagcg agactatcta caagaacctc tgcaagaagg tatttacgct cactacctac
     5401 tggtgcaact acaacatact ccatatctgg tcctactgtt aaaccgcgtg aagctgttac
     5461 agtaaatggt gttgtcgtcc aaacgacgat ataggtatct gtatcaagaa ttcccttacc
     5521 atctttaact ttattggcat agtagagtga tgtcgaatca atatcatgat attcgatttc
     5581 agcctcagca agggcagact ctgatgacca tgaccaatac actggtttag caccacgata
     5641 gatatatcct ttatctgcca tagcaccgaa aacacgtact tgatctgctt cataatctgg
     5701 tgtcagtgta atataaggat tttcccaatc agcagaaacg cccagacgtt taaaatcatc
     5761 acgttgtttg tcaacttggc tgagagcata atcacgacac atttcaaggt attcagccaa
     5821 gtccatttct ttacgtttaa cccctttttt agctaatact tgctcaattg ggagtccatg
     5881 agtatcccaa ccaggcacat aaggagctcg aaaacctgac attgattttg aacgtacaat
     5941 aatgtcttta gaaattttat ttagtgcatg tcctacatga atattcccat tagcataggg
     6001 aggtccgtca tgaaggtgaa aggctggttt tccttcattc aatgcttgac gttttttata
     6061 aatatcagct tgatcccatg cttcttgcca ttgaggttcc ttatttggaa gccctgcacg
     6121 cattggaaaa gctgtttgtc ctaaattcaa ggtttctttt aatttcatac taactcctta
     6181 tctcatagta atagttatca caatttttta aattgctaaa taaaaaagct ttcatcacat
     6241 aaaggacgaa agctcgtggt accaccttaa ttcagagcag aaaatagcta tttcttactc
     6301 cctcaacaac ccgtaaggtg ggttaagcct tctaccttac ttaaataata gttcaggtag
     6361 aatgattgaa aatgataatt tcaatattag ggattgtagg gctctcacca tctcctactc
     6421 gctgataata taatattaaa accttgtttt tcttaatctt caatatttaa tttaaaagtt
     6481 tgagtttcac ctaaattgat cggttcttcc atttggaaat tattagtatc taataaacct
     6541 gactcttcaa gttgtttgtt actttcttca acacgacgct gtaattcttc catttcatct
     6601 ggtgagaatt gacgagtagc atcaaatgac tcagtgtcat ccaccactgg taaagcatca
     6661 tcttcatcaa gaactttttc aacaacttct ttgaaagaag catcagaatt ttgaagataa
     6721 atagctgttg gtttcaacaa ttcttcccaa gcagatgagt tagctaattt taactgacct
     6781 tctaattcag aaagtaagcg ttggtggaaa acacgtgatt gacgtttcaa atcttctgtt
     6841 tctatagcga cacgcttagc atcatctgtc gcatcacgaa ggatttgatt tgcttttaat
     6901 ttagcctcat caattaaatg ttgagcatca aatgtagctt tccccattag gttagatgct
     6961 tcatcctgtg ctgaaatttt cacacgttca gcagtttctt gagctaaaat aactgattga
     7021 cttaacgatt ccttcatttc gttgaagtaa gcgattttct cttctaaatc tttaatgtat
     7081 tgctcttgct cacgattacg tctaatcaag tcttcgtaat cgtcaacaac aatctctaaa
     7141 aattcattaa cttcttcttc gctataaccg cgaaattttg atgaaaatgt tttatcttta
     7201 atttcaagtg ctgtaagtgg catgttttct ccttagttat gtatcatttt gtctacttct
     7261 aacttatatt tttgattttt agttaaccct aggttatggt tcaacgtaaa tcgaccatat
     7321 cctctaacac taatcaaatc cccctctact aattgttcag aaactcgatt gacgattgca
     7381 taattgacct tgactttatc tgcctcaatc aatttcgtcg actgtgttcg tgaaatcttt
     7441 aaaatcgttg ctaaaatttt atcaagacgt aaactggaag caataacagt tagtttttga
     7501 atatcttgtt tgggagttag aagtttggac aagggaactt cagctaattg tacactagca
     7561 gttccaattt ttgtaactga atgaacaagg tgatttgtca tttgactatc gaccaaaacc
     7621 tgagcacagc cttcttcaac taggatatcc cctaaaattg atcgttttac acctaagtaa
     7681 ttcagtaaag ttcccataat tttagcatgg gtaaggtgat taaatttagc attatacttt
     7741 atttcaatta ggctaaggtt aaaatcaacc atatctaact gatagtattc cggtgctatt
     7801 attactttaa catattctgt ttgaaaatac tgaccagaag tgtaataatg actacctcgc
     7861 ctctctagaa cactttcgag tattttaaat tccctaggat ttagaaattc agtggtaata
     7921 aacgaatagg tgttttcgac atattgagct aaatggtcta ttttattgat aaacgcatac
     7981 tcttctggtc gaaaatgctg ataaatatca tctaacgtca tcttatgtaa aaagcaagat
     8041 taaaaatcta ctcaacatat ttagtaaaaa gaatgctact attactgtaa aatctagacc
     8101 taaaaattgt agattaaatc ttctgagtgg gcgaataatc ggatcaacaa tctgtaccac
     8161 tagtctacca atccatgaat cgtatgctcc cggaagccaa gataacaagg cataggctag
     8221 taagatataa gaatagaccc taattgtttt taataatatt aatagtacta atatcatatt
     8281 tatcgtctct tcatatcaaa atcaaaactc gtctcttgac cagtctttgg aatattcatc
     8341 tcctcaatat cgaccataac attggctggt gttaatagaa acattgaact cccaaccttt
     8401 tgtaatgaac catataatac tctacttgca ccatcaatat aatccaagca acgtcgtgct
     8461 tgcgcatcaa gcatatattg aaaatcaatc aagacacatt cattaacgat aagtaaatca
     8521 acaatttctt gtgcatcctc atacttacgg ggatacttca gagcaattgt tacttgttct
     8581 ctctgttgtc gaactgagtc tttattttga atcctaggag tagcttgctg ataagcattg
     8641 tctctctgta ccctttgtct ctcctgacga ttaggattat aggtagaagt tctcggacga
     8701 ctaccaatca tctcttcctc tgcggagtta gtcatatgac tacgctgaga agcttcctgt
     8761 gctgtagctg ctctggaatc tctttgcact gaagttctct cttgtacttc gtgtacttca
     8821 ttttcactta catcgtcagt gtcaaaataa gaaataattt tgtcaaatct atcttttaat
     8881 gccatattcc cctccattat ttaaagaatg aagttcctat ccttacaaat gttgatccat
     8941 tttgaatagc aatatcataa tctctactca tgcccatgct taattctgta aacggcattc
     9001 gttctatgtt cctagcttga agccttttcc taagactctc agtctcttca aaaattgatg
     9061 atatttcctc tttagttgct tttaagggag ccatagtcat gatgccgata agttcaattt
     9121 tatcgtaccg agaaattaag ttcagtgcat catctatttg ctcaatagta aaaccatgct
     9181 tactgtcttc tcgtgatata ttaacttgta gaaaacattt aactggtttt tgagcacgtt
     9241 tttgaatctc agctgctaac ttaacagaat ctaaagcatg aaaataatca acataattaa
     9301 tgacatcttt aacttttcga cgttgtaaac taccaatgag atgccatgta agcttttcat
     9361 cttttaatgc ttgatatttt tcaagaaatt tatcaacacg attttcaccg atatgattaa
     9421 cacctgttct aataagcgct tctgttgttt gacagttaac atattttgtt acagctacga
     9481 tatgaactga ttcaggagcg cgacctgctt ttagtgctaa tttactaaca ttgtcaaaaa
     9541 tcgctgtttt attttcttgt aaattcatca ttttaattaa cggtttttaa agaatggagg
     9601 tgtttcaagt tcatcatcca tatcatcatt ttctgaaaac gttgacatag acaacttgct
     9661 atcaagttca ccttctgtcg gacgtgaaat attatcacga cgaaggtccc aattaccaaa
     9721 agcagaactt tgttgctgat tttgtgcatg aggctgattt tgttgagttg gcatttcgcg
     9781 agactctgtc atatcaaagt tgccacgacg atcaaagttt gaatttgaag tactttgacg
     9841 ttctgaaggt gcttgattag ttggagctga tgttgtaaaa ccagatactt gattagtttt
     9901 atctttacgc acacctgttg caacaactgt tacgcggatt tcgtctttca tatccatgtc
     9961 aattgaagtg cctaaccaaa tattgacacc tttacctgct gcttggctaa caatctctga
    10021 tgcttcctct gcttcagtaa gtgtcatatc cataccgccg gtaacattga caataacgtc
    10081 ttctgctcca tcaatcgttg tttcaagaag tggtgaataa attgcttttc gagcagcttc
    10141 agtaatacgt tcctcaccag aaccgatacc aattcccatc agtgcattac ctttatttgc
    10201 cataactgtt ttaacatctg caaagtcaag gttaattaaa cctgggttag taatcaaatc
    10261 tgtaatacct tgaacacctt gacgtaaaac attatctgct tcactaagag cttcaagcaa
    10321 tggtgtcttc ttatctacaa tttcaagaag attattattt gaaatgataa gcagtgtatc
    10381 aacttgttct cttaattctt gaataccttc gatagcgaag ttagaacgtt tattaccctc
    10441 aaagccaaaa ggacgtgtga taaccgctac tgtaagtgct cctaaactct ttgcaatacg
    10501 tgcaataact ggagccgcac ctgtaccaga tccgcccccc atacctgctg tgataaatac
    10561 catatcagcg ccagtaagtg cttctgttaa aacttcttca ctttcttcag ctgctttacg
    10621 accaacttca ggttgtcctc ctgcaccaag tccgcgagtt aattttggac caagttgaat
    10681 gacagtttca gcttttgagc tacttaaggc ttgaatatca gtatttgctg caataaactc
    10741 aacgccagcg acaccttcgt caatcatgcg gttaatagcg ttaccgccac cgcctccaac
    10801 accaataact ttaataactg caccttgtac tgatgctgta tcaaatgaaa ataccatttt
    10861 tattcctcac ttattgtttt agtcaaacat acttccaaaa attcctctga tgcgctcacc
    10921 aatattagtt ttaggctcag taggctcttg agcgttttct cttgctcgga ttggttctaa
    10981 attagaatct tccatagaag aggttaacgg ctcagaataa ggcattgtcg acattgtatt
    11041 tgttttttct ttataatcaa agtcgactgg tttatgtctt agcatttcgt cgccagttac
    11101 agcatgttgt gcgataatgt caacttctga catcattcct acataatcaa cgatactaat
    11161 aacattagcg aacattggat tgcgaatacc tacttggttt ggaacgtgca atttaactct
    11221 agttccaaaa atttgttgag caacctcgac aactcctggc ataattgcac cacctccaac
    11281 aagaataata ccaccaggta aatcaagtaa acgacctcta cccaagtctt gtttgacatg
    11341 ttctaaaatt tgacgaatac gtcctgaaat aatttgcgaa agatagcttt ctgtaatttc
    11401 aacaggttcc tcgttaccaa caacattaac ttgaactgta tcagaagtac ttgcttcttc
    11461 aacattagct tgtccaaagt tgaatttcaa tgcttcggca atttctactg ttgtacgtaa
    11521 cactttagaa atatctttgg tgacataatc gctaccttca gaataaatat tagtatattg
    11581 caattcttga ttacgcatag aagcgactgt tgtttgtccg cctcccatat caatcacagt
    11641 tgcaccaaac tcacgttcac cttcgtttag aactgatttt gctaaggcta gaggggcaat
    11701 cacaacatgc tctactttta tacctgctcg ttcaacagtt ttacgaagat tatgaaggat
    11761 agttgttggt ccagtataga taagaccacg catctcaagg cgaatcccca tcatccctct
    11821 agggtctcta atcccttgga aaccatctac aatgaattca agcggaatca atgaaataac
    11881 ttctctttca ggagtaatgc ttttagtcaa tgctgattta acaacacttt cgacatcttc
    11941 atccttaatt tcttttgatt cattgggaac aggaatcatt ccttgagttg gttcaatttg
    12001 gagaagattt gctggtaatc caacattgat tttgtcaatg gtgatgcctg ctttctcctc
    12061 agcttgtttt accgcctctt tgattgcagt tgctgctgcc tcaatatcaa taattatacc
    12121 atcttttaca ccggaactag ggacgttgct aaccccaatt acattcattt catttgcaat
    12181 aaattctgca actaaaactt taatcgagct tgttcctata tccaaaccag taaaaaagcc
    12241 atttctagcc attcactcga cctcgctatc tttccattct tttctattct acatctgcca
    12301 aaaacagaat tattcagaaa atattatacc ataaaaaaag cagaattgtt accaaattac
    12361 gtttttttat taagcaacat
//
LOCUS       DATROY010000054        12268 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.055, whole genome shotgun sequence.
ACCESSION   DATROY010000054 DATROY010000000
VERSION     DATROY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..288
                     /locus_tag="VBD11_001142"
     CDS             <1..288
                     /locus_tag="VBD11_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954102.1"
                     /translation="ILRIIENTKYIHHYDGKVVVEIPPQKEPIKVFFMDSRLHYLEFS
                     DICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLPSIQHGLFLNDD"
     gene            322..837
                     /locus_tag="VBD11_001143"
     CDS             322..837
                     /locus_tag="VBD11_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954103.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            889..3546
                     /locus_tag="VBD11_001144"
     CDS             889..3546
                     /locus_tag="VBD11_001144"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6954104.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            3592..6910
                     /locus_tag="VBD11_001145"
                     /pseudo
     CDS             3592..6910
                     /locus_tag="VBD11_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7094..8179
                     /locus_tag="VBD11_001146"
     CDS             7094..8179
                     /locus_tag="VBD11_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6954105.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIELKEKMLTPN"
     gene            8200..9018
                     /locus_tag="VBD11_001147"
     CDS             8200..9018
                     /locus_tag="VBD11_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6954106.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            9157..10188
                     /locus_tag="VBD11_001148"
     CDS             9157..10188
                     /locus_tag="VBD11_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6954107.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            10223..11222
                     /locus_tag="VBD11_001149"
                     /pseudo
     CDS             10223..11222
                     /locus_tag="VBD11_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            11257..>12268
                     /locus_tag="VBD11_001150"
     CDS             11257..>12268
                     /locus_tag="VBD11_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6954108.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNL"
BASE COUNT         4566 a         1983 c         2324 g         3395 t
ORIGIN      
        1 attttaagaa ttatagagaa tacgaaatat attcatcact atgatggaaa agttgttgtc
       61 gaaattcctc ctcaaaaaga gcctatcaaa gtctttttta tggattcacg tcttcattat
      121 ttagaatttt cagacatatg tcggtatgta actgttgttt tgaaaaatag taccgttgat
      181 aatataacca atttagggct agataatcat attgtttatg acaatggtga tgatatggtt
      241 gatccgttgc cgagtattca gcatggatta tttcttaatg atgattaaag atgtagacaa
      301 aaatttagaa acgaggaagc catgacattt tataaaaaga cggacaaatt tggatttagg
      361 aaaagtaaag tgtgccgcag cctatgcggt gctttattgg gtacagtagc agttgttagt
      421 ctagctacag caagtacaga gattcacgct gatgaagcaa caacctcacc taccactgta
      481 acgaaagtac ctcagccagt tcaagcagac acaacagctc taaatacctc taagactcac
      541 agtacacaag caactacaac tcctgttgaa gctaaggaga ataaggttgt caaaagtgaa
      601 actgttcaat ctgagagccg agtaatgcct agagataagg tagtagaaag gccagaaaca
      661 gtaaaagcta gtgttaactc agacgttagc caacctatta caaccacacc cccaacaatt
      721 aatgaaaaaa cagttgagat tcctaactta gctcaagata ctaagaaggt agctccaaaa
      781 gtaactgtta caccagaagt tgttaatcag ccaaagcagt tgcctaaata cgagtaagaa
      841 aactcagaga aaggcaataa gagtacagaa gcaactaagg cagttactgt gactaaacca
      901 cagggggcgg ttgctgagaa agctactccg gctgtgccaa agcctcaaaa agtaagagta
      961 attgttgaat ttaataagga atctatctta gattatgcga cagaacagaa aaaaacagtt
     1021 gcacagttaa atcaagctga tgttgagaaa aaattacaaa gcatcaagca agagcaagat
     1081 aaagttctta agaatattga aaaaagtgtt cactttgatt caagcaaagt gaagcgttac
     1141 gatgctatta ttaatggtgt cgcacttgat attcaagctc aagaaattga aaaattaaaa
     1201 acgattgctg atgttagaag ggtctatgtc tcacaagaat atgttcagac aaaaccttta
     1261 ctatcttcct cagggcaact tattggtttg ccagaagttt ggaacaactc ccagtataaa
     1321 ggtgagggaa ctgttgttgc agttattgat tcaggggttg atttcaaaca ccaagcacta
     1381 aaaatcaaag aaccaaatcg tgctaagtat aataaaacta gtattgaaaa actgattcat
     1441 gaaaagaatc tcaaaggaaa attctattcc gaaaaagtac cttatggtta taactattat
     1501 gactataatg ataacctcaa ggatagctac ggtgtaatgc atggtatgca tgtaacaggt
     1561 atcgtaggag ctaatgatga taatcaaaaa ttgtatggtg ttgcaccaaa tgctcaaatt
     1621 ttagcgatga aggtattttc agatgaccaa caaaatccca caaccttcac agatgtgtgg
     1681 ctaaaagctc tagacgatgc tattttacta aaagctgatg ttgttaatat gagtcttgga
     1741 acacctgctg gatttgtaca tgaggggaaa gactatcctg aacttgaagt tattgcaaga
     1801 gcgtgtaagg caggaatcgt cattgcagta gcagcaggta atgaaggaaa tatcactgac
     1861 ggtaatactt atggtgttaa gccgctagca gaaaattatg atacagcctt gattgccaac
     1921 ccagccctcg atgataatac cttggctgtt gcctctatgg agaaccttaa aaagcatgct
     1981 catgttttga aatttaaaga taaaaaatct ggaacagaag ttacagaagt cattaatctt
     2041 catgttgccc caaacgcttc aaaaactatt attggattgg ctgtagattt gggagcagaa
     2101 gctccttcgg aattgtcaaa acattttgat ttaagtggta aaatcgccat gcttgaaatt
     2161 ccagaagata ataaatctaa tggattttta gaaaaagtac aggcgattac taagttaaat
     2221 ccagctgcta tcttgcttta caataatgcg aaggttaaag atgatttagg cagtcaatta
     2281 ttagttgaat cagaagcagc taaatttaat attgctcgca ttaccagaag cacgtataac
     2341 aatatcaaaa ataatagcaa taaaattatt actattttaa cagagcgtca agctattgat
     2401 aatagtttag caggacaatt atcttcttat tcttcttggg gacctacacc agacttacgt
     2461 ctaaaacctg agattacagc tccaggtggt catattttct caactgtgga ggataatcaa
     2521 tatgctgata aatcaggaac atcaatggct gcacctcagg tagctggggc agctgcagtt
     2581 ttaaaacaat acattacaga taaaaaaatt cctgttgata atgctgctga ttttattaaa
     2641 ttactcttga tgaacactgc acagccaatt atcaataaac aatcaaaaga tggcaaaaca
     2701 ccttactttg tgcgccaaca aggtagcgga gcaatgaatc ttgccaaagc tcttgttacg
     2761 acagtcgttg caactgtaac agggactaac gataataatg cagatggtaa gttggagtta
     2821 cgtgaattaa aagagaaaaa atttaaagca cgcattctac ttcgtaactt tggaaaaact
     2881 aataaaacgt atatcatctc atcagaagcg attgcggacc cagttgatga aaaaggattt
     2941 cgtacgcaaa attcagaaca cctagtatca aaaaaagctg atgcagttac aaggaaagtt
     3001 actgtagaag caggtaagac attagctgtt gaccttgatg tggattattc tgatgcagag
     3061 gctttaactc gcaataattt cttggaaggt tatcttaact taaaagatac tgaaggtgta
     3121 gccgatttac acttaccatt tttaggtttt tacggttcat ggacagagca aaaagctatt
     3181 gatgcttttg aaggcatttc tgaaattggt aacggtgaca aaaaacgtcg cgttcaattc
     3241 tatgttaata aagaaaccaa caagacctca tcaaccttta caactaacgg catgttgagt
     3301 cttccaatct ataacaatac agtatttttc tctcctaata gtcctttcta tgacaaagca
     3361 ggagttcgta ttgcagcttt gcgtaatatg gagtatgttc aatattctat tatagatcca
     3421 gataccaata aagaagttcg tgtacttggt agatcacacg acgttagaaa actttatcgc
     3481 ctagattatc gtaatagttt tgccatgatg ccagattcta tttgggatgg taagattaaa
     3541 gattaaattg caaaagggga taaacaatac atttatcaaa ttaaagtaca gttgaataat
     3601 aaaggtgtcg gtggcgatgg tgtccaaatt tatcaatact atatcaaaat ggacaacaat
     3661 aaaccttact taagtcccaa agataagact actgtagaga agttagaaga tcgctggaaa
     3721 aaaattactt tcaaagttca ggatactggc attggtttga aagacgttta tcttcaatct
     3781 gttaagtatg ttggtggtgg caataataat ttagacctta tcacacctcc aggatttaaa
     3841 aaagaagata aaaaagttga aaaaccaaaa ttagaccgtc caccaggaat tgatttacca
     3901 gcaccaactt caatgagaag ttttgattat tcaaccccac cgggaactaa gccaagcaaa
     3961 cccaaagata gtttatcaac tcctccaggt ttcccagatt taaacacgcc gccggatgaa
     4021 gccaccaaag gatagtaaaa aagacgctat tgaagataaa tcaggagcaa ttaaatatgc
     4081 taagtctctt caacttagct ttgttgatga ccctatttta gctagcaaag taaatggcaa
     4141 aatattacaa gtcgaatctg atggcaaatt agtcattcct agaaatgctt tgtcagctaa
     4201 tcaatttgat gacactagtc ttaaaattta tcgtaataat aatcgcaata aagaaattac
     4261 tatcacaaca gattattttg cagatacaaa atatgtcaat atcacagcgg ttgactattt
     4321 gagcaatact acttttgagc aattagctac tggtgaaaca gtagattacc atgccattgt
     4381 attttcaagc tttgctgcta ttaaagacaa gggtggtaag atttatgtta acgataaatt
     4441 gcaagaaact tctcgtatag cgcttaaaga taaatctgtt aagattggta ttgaattacc
     4501 aaatgatgtc agacatattg atagtttatc tgttcgtcgt ttgaatgagg ttaaaactgt
     4561 tgataatatc ttgaaaaatg atgaacaaga cattaatctc agcaaaactt accaattaaa
     4621 atacaacccg acaaatcgtc gtctagagtt tactattaat aacattaact caagttcaga
     4681 aatcatgacc actttcaaag atggaaagat gccagaattg gttgaacaaa aagatgtttc
     4741 tttggatata aacgatatgg acatgagtaa gtttaaaact attcgacttg gacgaaagga
     4801 ttctgaattt aagggacaac ttattgcaaa aactggaaca gttgaattag atatgttttt
     4861 caaacaatct caagacccag cttcaattat taaaaaaata taccttatcc aaaatggtgt
     4921 tccaaatgaa ttgaaaaaat ttgactctag ttttggttta actgaaagtc agatagatgg
     4981 atactatatt tataaagatg caattaacct taaatttaaa ttaaccagtg gtgcaagtct
     5041 taaagttgtt tataaagggc aagaagatcc atatagtcat cagaaagaag atatgactaa
     5101 aaaaggtgaa cagctcagtc attcaactca agccaatgaa aatacagcaa aagtaacctt
     5161 tgctaatatt gactggtcac attatagtaa ggttactgtg aatggaaaag aagttggtaa
     5221 aggtagtgag ttacctttaa ctaaaggatg gacaacattt gtattacata aaacagaaaa
     5281 ttcattaaat gttaaaagtt tgattatgga gacgggtagt gtaagtaaga aagttcaaca
     5341 acttccttta agtcctagat tatctaaaaa taagcatatg agggatatgc tacttactat
     5401 gcaaaaagat tcagcgtatt acgaaacaag tgacagtcta gtccttcgaa ttaatctcac
     5461 tgcagatact aaacttaatt ttaatgctgt taaaggagcg agtgctctta ctgaaaatat
     5521 gatgatgaga cagtttgcag ttgctggacc acaagatgat cctgttagtg aacataaata
     5581 cccatcagta tttctcttaa ctcctgcctt attggaaact gctagtgagg caactctaaa
     5641 tggtaaggaa atcacagcat ctggtattat cggtcacatc aaggatggtg ataaaagcaa
     5701 gcatgttgaa gtcaaaatgg tgaatgaaaa tggagacatg ctaggaaccc ctgttattat
     5761 tcaaggtaaa gacttgacta atcgaacaaa accattaatg agtggacgta gagtacttta
     5821 tgccggtaaa caatatgagt tccgggctaa attaccactt agtcgtttta acacttggat
     5881 tagggttgaa gtggtaacag aagcaggaga gaaagcaagt attgttcgtc gcatgttctt
     5941 tgaccaatca gttccagagc ttaacacagc agttgctaaa cgtgatttga cttctgatac
     6001 tgctcttatc cacatcgttg ccaaagatga ctctctaaaa ctaaaattat atcaagatga
     6061 ttcattactt gaatctgttg ataaaaccgg tctttatagt tttagaaatg gtgtagaaat
     6121 cactaaagat atgacagtac cactagaatt tggagataat attattaagt tatctgctgt
     6181 tgacttatca aattatcgtc gtaatgagac ccttcatatc tatagaaacc gttttgatgt
     6241 taaagcaagc caaatgacag ctgacaaagg agctaaagta actgtggata tgttgatgaa
     6301 gcacttagtt gttccagaaa tggcaggagc ttatacatta acaatcgacg aagctccaaa
     6361 cacaaatgaa tcaggaatgt taacaaacgc taaagtatcg attcattatg taaatggtgg
     6421 tgttgataaa gttgatgttc cgattaaagt agttgactta gaagctattc gtaaagctga
     6481 agaagcacat aaagctgacg aagcacgtaa agctgaagaa gcacgtaaag ctgaagaagc
     6541 acataaagct gaagaagtac gtaaagctga agaagcacat aaagtcgaag aagcacgtaa
     6601 agctgaagag ggacataaaa cccaagaagc acctatagtt gaagaaggct acaaagttaa
     6661 taacgttcat caaactgata ctacagttaa agcgtctgat ttaccaaaga ctaagacagt
     6721 ttccgcagtt catatggcta gaacagacaa taaacagata acttcacatc agacacatgt
     6781 tgaaaaacaa attaaaaata cattgccatc cactggtgac agcaaacgtg gttattatat
     6841 cactggaatg gctatcgtta tgctgagtgt attatttagt ttagctaaaa agtttaaaag
     6901 caaatattaa tgataaaata aaatctattt agcaacatta attgtgatgg ataaaaggtt
     6961 ttatctctta aatcaaaagt agtagctcat tagctactac ttttttgtat aagtgtaaaa
     7021 aagtgaattc ttaacaagaa tttaacaccc tacttgctat actaaaagtg aataagaaag
     7081 agaggtgtca tttatgcaaa gaaaatccta tttaaaatcc atgagtgttc ttactttaac
     7141 agcttgtctt atatcaggat atgtggttaa agatattgct atgttacatg cagtatctgc
     7201 cagtgagaag aaagcaaata atgtcagtcc gagagaaaat ctctacaggg ctgtcaatga
     7261 taattggcta gccaatacaa aactcaaaca agggcagact agtgttaata gtttttcaga
     7321 aattgaggat aaattaaagc aactgttagt gtctgatatg gctaaaatgg cctcaggaaa
     7381 gattgaaaca accaatgatg aacagaaaaa aatggttgca tactataaac aaggtatgga
     7441 ctttaaaaca agagataaaa atggtctcaa acctctaaaa ccagttttac aaaaacttga
     7501 agcagtctct tcaatgaaag actttcaaag tttggcccat gattttgtga tgagtggttt
     7561 tgttttacca tttggtttga ctgtggaaac caatgctcga gataatagcc aaaagcaatt
     7621 ggtgcttcgt caagcacccg cattacttga atcacctgac caatataaga agggcaataa
     7681 agaaggtgag gctaaattat cagcttaccg tacttcagca atggctttgc ttaaacaagc
     7741 tggaaaaagt aacattgaag atagaaaact agttaaacaa gctatagcat ttgatagact
     7801 cttatcagaa aaaacgcaag ttgatcaaag taaaatcaca gctgaaagtg agacagctgc
     7861 ggggcgatat aaccctgaaa gtatggaaac ggttcacaat tacgccaagg aatttgactt
     7921 taaagaattg attgaaaaac tagttgggcc aacgaataag gcagtcaatg tagaagataa
     7981 aacttatttt aaacaggtta atgatgttat aaatagtaaa caattagcca atatgaaagc
     8041 atggatgatg atttctatgc tagttgatca atcagatttt ctaggagaac aaaatcgtca
     8101 agcagcgagt gcttttaaga atgttgcgtc tggtttgact cagattgaat tgaaagaaaa
     8161 aatgcttaca cccaattaga atatactttt ggccatattt tgggtgatta ttatggtaag
     8221 aaatattttg gtgaggcagc taaaaaagac gtcgaacata tggctaagaa aatcattaat
     8281 gtctataaaa cacggttaaa aaacaacact tggttatcag aaaatacaaa agcaatggcc
     8341 attaagaaac ttgataacat gagattaatg attggctatc cagaagatta tcctgatctt
     8401 tatcgtcagt accaatttga tagtaaagca agcttctttg aaaacaatga taactacaga
     8461 aaattatcga acaagaaaac atttgaagaa tttaaccagt ctaatcaacg tgaacattgg
     8521 caaatgagtg ccaatgctgt aaatgcttat aatgatccta ataccaattc catagtcttt
     8581 ccagcagcga tttttcaatc accactctac gataaaacta aaacagttag tcaaaattat
     8641 ggagctatcg gagcaattat tggtcatgaa atttcacact catttgatat taatggtatg
     8701 aaatatgacg agaaagggaa tcttcacgat tggtggacta aagaagattt aaaacattat
     8761 aagaaaaaaa cacaagctat gattgaccaa tgggatggcc ttaaagcaga tggcggtaaa
     8821 gttgatggta aattaacttt agcagaaaat attgcagata atggtggtgt tatggcatct
     8881 ctagaagctc ttaagactga aaaaatccaa actataaaga attttttgaa tcatgggcaa
     8941 gtatttggcg tcaaaaagca accaaagaac aaagtaagtc ctcaattcag tcagatgttc
     9001 atgcaccata tgaattgaga gctaacatcc cagtacgtaa tttccaagaa ttttatgatg
     9061 cctttggtgt taaaaaaggc gattcaatgt atctaaaacc agaaaaacgt ttgacacttt
     9121 ggtaaagcta tgttttccag acaataagga gaatgcatga agaatataac aaagctatca
     9181 actgttgctt taagcctact actttgtacg gcgtgtgctg catcaaacac gtctacatct
     9241 aaaacacagt ctcatcatcc taaacaaact aaactcacag ataagcaaaa agaagaaccc
     9301 aaaaacaaag aagctgctga tcaagagatg catccccaag gcgctgttga tttgacaaaa
     9361 tataaggcaa aaccggtcaa agattatggt aaaaaaatcg atgttggtga tggcaagaaa
     9421 atgaacattt atgaaactgg tcagggaaaa attccaattg tttttattcc tggtcaagct
     9481 gagatttcgc cacgctatgc ttataagaat ttaattgagc ggttaagtaa aaaatataaa
     9541 atttatacag tagaaccatt aggctatggt ctcagtgata ttcctactaa accacgtacg
     9601 ctagaaaaca ttacaaaaga aattcacaca ggcctaaata aaattggagt taaaaatttc
     9661 taccttgcag ctcactcact tggcggtatg tattctttaa attatgctaa aaattatcca
     9721 gaagaagtca gaggatttat tggtatggat accagtacac cttggatgga aggggaacaa
     9781 aaaacaaagt atgaccctga aagtgctaaa tgggcaatga aattaccaga tgttgatgat
     9841 aagactaacg aacagtatct gtcaattgcg aaaaaaatta acggcaataa gtctcaacga
     9901 gatgaagatg agcgcttagg tgataatctt ggtaaattga agaatgtaaa attccctaaa
     9961 gggatgccaa ctaagtattt tcttgctgat caatctgtgg agggaatcaa gctacgtcaa
    10021 gcagatttta aaggtttgaa aaattgggat cagcagcata ttgatttatc tgaagatcca
    10081 aaagatgctt ccgttgaaat tcttaaaggt agtcacaata ttttccaaac acaatatgaa
    10141 catatggcta aagctattga tggatttatc caatcccatc aaaagtaatc gttgaatgaa
    10201 atacctaagt caaggagaaa aaatgaataa aataacgaaa ttatcaaccg ttgccctcgc
    10261 tttgatgctt tgcgttggat gttctgccaa taaagataat caaaaaacta aaactgagca
    10321 gcctaagcaa gaaaagtcaa tcgctaagca aaaacaagag agagaagcag aaaaagctat
    10381 gattgctaaa ggtgctattg acctaactaa atataaagcg aaaccaattg aagattacgg
    10441 acaaaaaatt gatgtcggtg acggtaaaaa aatgaacata tatacaatgg gtcaagggaa
    10501 aattccgcta gtagtcttcc ctggtcaagc tgaaatttca ccacgttatg cttacaaaaa
    10561 cttgcttgat agattaggga aaaaatataa aatttatacg gttgaaccgc tcggttatgg
    10621 gcttagtgat atgacagata agccacgtac gattgaaaat attaccaaag aaagtcggtg
    10681 tcaaaaattt ctatcttatg gctcactcgt taggtggcat atacggtcta aattatgcta
    10741 agacatattc taacgatgtc aaaggtttca ttggcatgga tacaagcaca ccaaatatgg
    10801 aaggtgatgg cacaactgga gaatcttcag cttcaacctt aaaatgggca gaatccattc
    10861 cagaagttga taagaaaacc aatgaacaat acttatcaat cggtgctaaa aaatcaggta
    10921 ataaaacaga gcgcgatgaa gataggcgtg ttagtgacaa tcttcataaa atggaaaaag
    10981 ttaagttccc taaaggtatg ccagctaagt atttcttagc taatgagtca gcagaagata
    11041 taaaaatgcg taaaaaaata ttccctaagc ttaaaagctg ggaacaacaa catattgact
    11101 tatctgaaga tccaaaagac gtttctgttg agacactaga tgctagccac ttgatttatc
    11161 atacgcaata taaacatatg gcgaaggcta ttgacgagtg gattcaatcg catgaaaaat
    11221 aattattccc cccaattatt ttactaagga gaaaagatga ataaaataac gacattatca
    11281 accatcgccc tgactttaat gctttgcgtt ggatgttctg ccaataaaga taatcaaaaa
    11341 actaaaactg aggatcctaa gcaagaaaac gtaactgcaa atccgaagga agacaaggca
    11401 acagcagcca agcgaatgaa gttaacgaaa gaacttgaaa aatataataa aggcacagag
    11461 agctttatta gtaacaaaca atatcaaaaa tatatcaaaa cacctatcaa agaatatggt
    11521 caaaaggttg aagtcaaagg caagaagatg aatgtctata ctgttggaga aggtaaagta
    11581 ccaattgtct ttattcctgg tcaagggacg gtgactgcca aacatcagta ccataatttg
    11641 attagtaatt taagtaaaac tcataaggtt gtggtagttg agccatttgg ttcaggttta
    11701 agtgatgtca ttgaccaacc acgaaatcta gctaatatta cctcagatat ccacgaagct
    11761 ttacaaaaag ttggtattac cggaaaatat gtaattgcct ctcattcaat tggtggtgtt
    11821 tatgccctta aatacatcag tacttatcca aaggaagtgc taggattaat aggtttagat
    11881 acatcaacgc cgggtatgga aggtggtaag caagttgatt tcgctgctcc agtgttaaaa
    11941 gaattaccta aaattcctaa agtatccgac gatattaatg cacaattttt tgctattgga
    12001 cataagatac ttaataattc taatatgaaa gaagaagcga agaattcttc taatatgatt
    12061 aacgaaagtg ccaactataa gattccaaag ggaattcctg ctatgtattt attagcacgt
    12121 gaaagtgact tagggcttca attacgtagt ttagatatac caattaaagg cagttggcaa
    12181 gagcaacata aggagttatc agaagatcca aaagaagtct ctattcatac actagacgga
    12241 actcaccaaa tttatgatac taacctta
//
LOCUS       DATROY010000055        12118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.056, whole genome shotgun sequence.
ACCESSION   DATROY010000055 DATROY010000000
VERSION     DATROY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..471)
                     /locus_tag="VBD11_001151"
     CDS             complement(<1..471)
                     /locus_tag="VBD11_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO6954109.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKK"
     gene            623..1483
                     /locus_tag="VBD11_001152"
                     /pseudo
     CDS             623..1483
                     /locus_tag="VBD11_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(1528..4212)
                     /locus_tag="VBD11_001153"
     CDS             complement(1528..4212)
                     /locus_tag="VBD11_001153"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6954110.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(4556..4954)
                     /locus_tag="VBD11_001154"
     CDS             complement(4556..4954)
                     /locus_tag="VBD11_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6954111.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            5018..6334
                     /locus_tag="VBD11_001155"
     CDS             5018..6334
                     /locus_tag="VBD11_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6954112.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            6406..7218
                     /gene="yidA"
                     /locus_tag="VBD11_001156"
     CDS             6406..7218
                     /gene="yidA"
                     /locus_tag="VBD11_001156"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6954113.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            7203..8438
                     /locus_tag="VBD11_001157"
     CDS             7203..8438
                     /locus_tag="VBD11_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6954114.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKDDQLVAVASILIKSLPLGFTMLYIPRGPIIDYSNKELVGFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQRDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNGIVSANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            8451..9662
                     /locus_tag="VBD11_001158"
     CDS             8451..9662
                     /locus_tag="VBD11_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6954115.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFELDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            9681..10916
                     /locus_tag="VBD11_001159"
     CDS             9681..10916
                     /locus_tag="VBD11_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6954116.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            11104..12036
                     /locus_tag="VBD11_001160"
     CDS             11104..12036
                     /locus_tag="VBD11_001160"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6954117.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIFHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
BASE COUNT         4056 a         2307 c         1987 g         3768 t
ORIGIN      
        1 tttcttctct gggttacggt caatctcagc taaaagttct tcgataatgt aagcaaactt
       61 acctggcaat tgcttacgta ctttagattt tgtatattta ctagaaaaat attttaaaac
      121 ttgtatttgt aacggaatca tttcccataa cttcgtttta taagcactta ctgacaagtt
      181 cattttatcg aaggctaatt tttcctttgg gtaataaagc agtatacaaa aatcatctaa
      241 atcaacagca actattttct gccaatcaaa gcagtctaat aatttagctc taatagaccc
      301 tgcacctgtc cgtaagagat aatcaacagc atcgtattcc ccgtgtaaat cactaataaa
      361 atattccgtc cctttgggta attgacaaat agcttctaaa ttgatcattt ctgttacgat
      421 atcttctttt cgaggaaatt tttctttcaa gagcttataa aaattagaca tgagacctcc
      481 ttaaggcacc ttaaatataa agcttctaaa cacaattatg aaagcgaatt ctacaatgac
      541 atatctatta tagaatgact ttaaaactat ttcaaaaatt tactgtttat ctctttgaac
      601 tacaatatgg tacaataccc ttatgaccaa acttgctatc atgagtgatt tacatattga
      661 cctcaatcat ttttctacct ttgagacgaa aacgttaatt aatttgctta agaaagaaaa
      721 aattgatcac ctccatatcg ctggggatat atctaatcat tttactaaag atacacttcc
      781 ctttatcaat aacttaaaaa agcatattaa acttagctat aatttaggaa accatgatat
      841 gcttgattta acagaaacag aaatccaacg ccttgacttt caaacctatc gatttgacaa
      901 aaaaatgcta ctagcttttc atggctagta tgattacagc ttttcaaata atagagatat
      961 caaggatgtt gaaaaattaa aaaagacttt ttggtttgat agacgtttaa aacgtccaaa
     1021 taatgatgta actatccaag catcaatttt aaaacgctta gacgaaatat tagcaaaagt
     1081 cgattcttca aatatcatta ttgctatgca ctttgttccc cataaacagt tcacgatgac
     1141 tcatcctaga ttctctcctt ttaatgcttt tttggggagt caggcttatc atgatttatt
     1201 ccaaaaatat catatcaaag atgttgtttt tggtcatgca caccgctcat ttggcgacgt
     1261 taaaattgga gagactactt atcattctag accacttgga tatattagag aatggaattt
     1321 aactattgac tttgtcaatc agaatcccaa tcataaccct aatttaacct ggaatcttag
     1381 caaaaggcat aatgccgtta agcatttgga tagttttgaa aactatccaa aaaaatattt
     1441 tgaagacgaa ttacgcaact ctatgacaat atttgattgc taaaaaactc acttagatat
     1501 tctaagtgag tttttttatc agctaaacta aatagctttt tcgtcttgtc ctaatttacg
     1561 ttggataaat ttaacaattt ccgttaacac taccatcaat aaacttccga taactacagt
     1621 taaccattgt gttgaactta aatgagttac atggaaaagt ttattaaatc caggaacaac
     1681 aatagtcacc atcaaaagaa tgaaagctac tggaatagac cagttaaagg ttctattttt
     1741 aaaagctcca actgtaaaga ttgattggta aactgattta acgttaaatg catgtaccaa
     1801 ttggattaaa ccaagcgttg caaaggccat cgtaagggca tctgcgtgaa tcatgcgata
     1861 accagcatgt tctggataca tcaatgccca accatagaca ccaagtacta agatagtttg
     1921 taaaatacct tgatagataa tagctcccat aacaccacca tcaaagaagt ttgactggcg
     1981 accacgaggt ttatgtgtca taactcctgg ttcagctggc tcaactccaa gagcgatagc
     2041 tggaagagta tctgttacca agttaatcca aagaaggtga accggtgcaa gtacatccca
     2101 tcctaataaa gttgcaaaga aaattgtaaa tacctctgcc atgttggctg aaagaagata
     2161 ttgaatagat ttttggatat ttgagaagac tttacgtcct tcctcaacag ccacaataat
     2221 agttgcaaaa ttatcatcag caagaaccat atcagaagca cctttggaaa cttctgtacc
     2281 agttattccc ataccaatac caatatctgc tgtttttagc gatggagcat cattaacacc
     2341 atcaccagtc atggcaacga ctttgccatc gttttgccaa gctttcacga tacgcacttt
     2401 atgttcagga gaaacacgtg catagacaga atattgttta aatacttttt gaaactcttc
     2461 gtcacttaat tcattaagtt cagcacccgt aaagacatga tcttctgtgt catttgcatc
     2521 aataatacct agacgctttg caatagcttc tgcagtatct tgatggtccc cggtaatcat
     2581 aatcggacga attcctgctt ctttagcaac tcgaacagct tctgccgctt ctggacgctc
     2641 agggtcaatc attcccacta aacctgagaa tactaaatct gactcaacaa tatcagtctc
     2701 taaagttgga atttgtgttt catatttata agccatcatc aagacacgaa gagcttgttt
     2761 tgcgagttct ttattggtat ttaggatagc ctctttatct tcagccgtga tatcacgaac
     2821 caagccatta tcttcaattt tagtaactcg ttttaataat tgatcaggag cacccttgac
     2881 tgcgataaag tagcgaccat ctgattcttt atgaatcgta gacattaact tacgatctga
     2941 atcaaatggt aactcagcga cacgtggttc atttttcaaa acttcacgga catcaaaatt
     3001 cttatctaaa ccaaattgta ccagtgcagt ctctgttggg tcaccgatta attttcctga
     3061 aggatcaatc ttagtatcgt tggagaaatt cataatacgt aaagtgttat tatcaactga
     3121 aatttcttcc gaagatgatt ggagaacgcc attagtatac actttttcga cagtcatttg
     3181 attcattgtc agagtaccag tcttatccga tgcaatgatt tctgtagaac ctaaagtttc
     3241 aactgcaggg agtttacgaa cgatagaatt acgtttagca agagtttttg ttcccattga
     3301 taagacaatc gtaacaattg caggaagccc ttctggaata gctgcaactg ctaaagcaac
     3361 tgaagtcatc aatccttcaa tccaaccttc tttacggaca aatatcccca ctaagaaagt
     3421 aataaccgca ataatcacga ttaagtaagt caataattta gaaagttgaa ctaaactttg
     3481 tttcaatggt gtttcagatt catcagcatt tgccaacata tcagcaattt taccaacttc
     3541 agtatacatc cctgtgttag taacaacacc atatcctcga ccatacgtga catttgaatt
     3601 ttgatacgcc atattcacac gatcaccaat acctgcatcc tctgctacta cttgagatat
     3661 gtctttctca acaggtactg actcaccagt tagagctgct tcttcaattt ttaaagacgc
     3721 ggcttctaaa agtcgtaaat ctgcgggaac aacatctcct gcttctaaca taacgaggtc
     3781 accaggaact agttctttag aatccacctc aatagtatga ccatcacgac gaacacgcgc
     3841 aattgggctt gacatatctt tgagggcttc aatagctgct tcggcttgcc cctcttgata
     3901 aacaccaaaa gctgcattta agataacaac tgccaagata atcaatgcat ccgttaagcc
     3961 atgcatacct tccgtaataa cagagagagc tgcagcaacc agtaagataa taatcattaa
     4021 atctttgaat tggtcaaaaa acttagctat tagactacgt tttttccctt cttcaagctc
     4081 attgcgacca tacatctcta agcgattttt cgcttctgtt gttgataatc cttcacgaga
     4141 agattctaaa gacgttagaa cctcttcttg accttgtgtg taaaacaagg attttttctg
     4201 ttcttttaac aaaacctttc ctcctttggt ctctccacaa acaaaaagag acctgttttt
     4261 atgaaaacaa gtctcgctgt ttaagacaac gccggaaatt tctttcgtaa tgacgacatt
     4321 gccacggtaa accgtctgct actcccttga tatgttaatc attgtaacag aaaaaaatcc
     4381 atttttcaat taattatcct tgctatcact aataatctca agggaaaaaa cgaagttcat
     4441 ctagtcgaaa taaaccatac tgcagtttat caaataaatg gctctcataa agagatccca
     4501 gcgtcttcag tagacgaaat tcataatgac tactcttttg ttaatggtaa ttatctcaat
     4561 accattgaat ctttaattca taatttgcaa aacaggctcc tgtctccgtt tgcttaagga
     4621 cataattgat gtgcaacctt tgctgtgaca aatcaaactg atagtgactt gtctctgtaa
     4681 tcaaatgttg aataccaagt ggagtgggga tattaaccaa agcaggcgtt tcacgatgaa
     4741 aacgcattac agacttagga tttgaaaacc tcgtcataag caattcctca tgatttgcct
     4801 taataactac tctctcgccc tcagcattaa tataattcaa ataaacgtaa cttccttttt
     4861 caataaattg acaatcgtga atctcatgaa tcatttcggt ttcgttgtca atttgaatgt
     4921 tattgttaat ttctagtttc atgacttctc tcacttcttt tatccttcta ttctaccaat
     4981 ttctgactca agtttcaatt tttgctataa tatatatatg aatgaaaaag tatttcgtga
     5041 ccccgttcac acttatatcc atgtaaataa tcaagtcata tatgacttga ttaacactaa
     5101 agaatttcaa cgtttacgac gcattaaaca aacctcaacg acttctttta ccttccatgg
     5161 tgcagagcat agtcgttttt cacattgtct cggagtttat gaactggctc gtaaagttac
     5221 agaaatcttt gatgaacatt attctgatct ttggaataaa aatgagtccc tgctaacgat
     5281 ggctgccgcc ctactacatg atatcggaca tggcgcatat tctcatacat ttgagcgtct
     5341 cttcaatact gatcatgaag cttacactca ggaaatcatc accaatccta ccacagagat
     5401 taatgctatt ttacgaaagg tggctcctga ttttcctgat aaagtagcta gtgttattaa
     5461 tcatagctac cccaataaac aagttgttca attaatttct agtcaaattg attgtgatcg
     5521 aatggactat cttctcagag attcttacta cactgctgct agctatggac aatttgattt
     5581 aacacgaatt ttacgcgtta ttagaccaac cgattctgga attgcttttg ctcgtaacgg
     5641 gatgcacgct gtggaagatt atattgttag tcgcttccaa atgtatatgc aagtttattt
     5701 ccatccagcc agtcgtgcta tggaattact cttacaaaat ttactaaaac gtgctcgctt
     5761 cttatttgat acccatcgcg atttttttga acaaacttct ccaaatctta ttcctttctt
     5821 cacggatcaa tatgatttac aagattattt agccctagat gatggggtaa tgaatactta
     5881 cttccaatct tggatgcaag ctgatgataa cattttggca gatttagcta atcgttttat
     5941 caatcgaaaa gtctttaaat caattacctt tgaagaggct gataaagaaa atctagttaa
     6001 aatgaaagaa ctagtctcac aggttggttt tgatcccgat tattacactg gtgtccatgc
     6061 taattttgac ttaccttacg atgtttatcg gcctgaacat tcaaatccac gaacagaaat
     6121 tcaaattatc caaaagaatg gacaacttgc tgaattatca agcttatcac cgattgtaaa
     6181 agcattaact ggatctaatt atggtgatca acgtttttat tttccaaaag aaatgttgac
     6241 attagatagc ctattttcaa gtacaaaaga agaatttcaa tcttatatca caaatgagca
     6301 tttgacactc acgaaagata atagccattc atgacaaacc ctttattttt tagtaagata
     6361 taaggtgagt attttaggat attgaaataa agagtgggag ataaaatgac ttcaattaaa
     6421 cttgttgccg tagatattga cggcacccta ttaaatagta agcgtgaaat tacacctgaa
     6481 gtggctaaag ctgttcaaga agcaaaatca aaagacgtta aaattgttat tgctacagga
     6541 cgtccaatca tcggagtcca agatctttta gaagaattga aactcaatga agagggagat
     6601 tatgttatca cctttaacgg cggtctagtt caagacacag ccacaggaga tgacatcatc
     6661 aaagaaacct taacctatga agactatctg gatttcgaac tgttagcgcg taaattaggc
     6721 gtccacatgc acgccattac aaaagaagga atttacacag cgaatcgtga tattggtaaa
     6781 tatacaatac acgaagcaac tttagttaat atgccgctct tctatcgtac tcctgaagaa
     6841 atgggagaca aagaaatcat taaactaatg atgattgatc aacctgacat cttagatgca
     6901 gcaattgcta aaattccaaa aaaagttctc gataactaca ccattgtaaa atcaactcct
     6961 ttttatttag aaattttacc caaaaacgtt aataaaggca ctgcacttct ccaccttgca
     7021 gagaaaatgg gattaactgt agatcaaaca atggctattg gtgatgaaga aaatgaccgt
     7081 gctatgcttg aagttgttgg aaatcctgtt gttatgcaaa acggcaatcc tgaactaaaa
     7141 aaaatagcaa aatatatcac caaatcaaac gaggaaagtg gcgtagccta cgcactacgt
     7201 gaatgggtaa taaactgatg tatcatgtta ccgttggaat atccgaaaaa gaatatgatg
     7261 cctttgctat tgcatcgtct caaaccaatc tcctccactc ttcaaagtgg gcacaagtaa
     7321 aatcaaattg gcaaaatgaa cgtttaggct tttataaaga tgaccaatta gttgctgtag
     7381 ctagcatctt aattaaatca ttacctcttg gttttacaat gctttatatt ccacgtggac
     7441 ctattataga ctacagcaat aaagaattag ttggctttgt gttaaaaaca ctaaaaaact
     7501 ttggacgaaa gaaacgtgct gtttttgcaa aattcgatcc agcacttctt ctacgtcaat
     7561 atcaccttaa agaagagaat gtcgccgaag agatagatga aagtagacag gcaattgata
     7621 atcttaagtc agcaggtgcc caatggatag gtccaacgaa agccatttcc gaaaccatcc
     7681 aaccacgttt tcaagcgaac atctatacca aagcaaatat agaagaaaac tttccaaaac
     7741 acactaaacg tctgataaaa gatgcaaagc atcgcggtgt tcaaatttat cgtgccaaca
     7801 ttgatgattt acctaaattc gccacggttg tagcattaac cgagaatcgt aaaggtgtag
     7861 ctttacgtaa tgaaaactat ttccatcaac taatgacaat ttatggagaa gatgcctatc
     7921 tctatttggc taaagtcaat cttcctaaaa gattagcgca atttaaagaa caacttcttc
     7981 aaatccaaag agatttatca gaaactccat cccatcaaaa aagtcgcctg acaaggctaa
     8041 atcaacaaga agcttctgtt aaacaatata tccttgaatt tcaggagttt agcaagaaat
     8101 atccagatga gcctgtcatt gcaggaattt tgtccattcg ttttggcaat gtcctggaga
     8161 tgttgtatgc tggtatggat gatagcttcc gtaaatttta tccacagtat ctcttgaatg
     8221 ctagggtgtt cgaagatgct ttcaaaaacg gtattgtatc tgctaatcta ggcggtgtcg
     8281 agggaagtct taacgatggt ttaacaaaat ttaaaagtaa cttcaatcca atgtttgaag
     8341 aatatattgg cgagtttaat ctagcaatca atccattatt atataaattg gccaacctag
     8401 cctatactat tcgaaaaaaa caaagacact ctcactaaga aaggaaactc atgtatagag
     8461 aaattaccgc tgtcgaacac gatcgctttg tgagcgaatc caaccaaaca aacctacttc
     8521 aatcttctaa ttggcccaaa gtaaaagaca actggggtag tcaattactt ggcttttttg
     8581 acggtgaaac ccaaattgcc agcgctagta ttctcatcaa atcacttcct cttggcttct
     8641 ccatgctgta tattccgcgt ggaccaatca tggattactc caatctagat attgtaacta
     8701 aggtccttaa ggaccttaaa gcttttggca aaaaacaaag agctctcttt atcaagtgtg
     8761 atcctctcat ctatttaaaa atggtcaatg ccaaagattt tgaaaattcg cccgatgaaa
     8821 aagaagggtt aatcgccatt gatcatttac agcgtgcagg tgctgattgg actggtcgga
     8881 caacagattt agctcatact atccaaccac gttttcaagc aaacctatat gctaatcaat
     8941 ttgaacttga taaaatgtct aaaaaaactc gtcaagctat tcgaacttcc aaaaataagg
     9001 gagtagatat acaatttggt agccatgaac tacttgaaga ttttgcagag ttaatgaaaa
     9061 aaacagaaga ccgtaaaggg ataaatctta gaggaattga ctactaccaa aaactccttg
     9121 atacgtatcc taataactca tacatcacaa tggcttcgct agatgttgca aaacgattag
     9181 aaaaaataga aaaagaatgc cagatagctc aatctgaaag aataaaatca cttgaactta
     9241 atcgtgagaa aaaagttaaa caacaccaag atacgattga tcgattaaat aaagaaattg
     9301 attttctcaa agaagctcaa aaagcctatg accgagacat tgttccattg gctgcaaccc
     9361 tcactctaga atttggtaat acatcagaaa atatttatgc tggtatggac gattacttca
     9421 aatcttattc tgctcctatt tacacttggt ttgaaacagc tcaaagggct tttgaacgtg
     9481 gaaatatttg gcaaaatatg ggggggattg agaatgactt atctggtggt ttatatcatt
     9541 ttaagtctaa gttcgaacca atcattgagg aatttattgg agaatttaac attccagtga
     9601 acagattgct ttataaagcc tctaattatg tctatgcctt acgcaaaaaa cgtaatagct
     9661 agttaggaaa ggatactttt atggcgctta aagaattaac agctaaagag ttcgaaagtt
     9721 attcaggaaa ttacgattta caatcgttta tgcaaacccc tgagatggca aagcttctaa
     9781 aaaaacgtgg gtatgacatt acctacatgg gctaccaaat cgatgggaaa atggaaatca
     9841 tcagtattgt ttataccatc ccaatgactg gtggacttca tatggaagtc aattctggtc
     9901 cagcccattc caattctaaa tatctgaaac atttttataa agaactacag aactatgcca
     9961 aatctcaggg cgctttagaa ttattaatta aaccttatga tacctaccaa gagtttactg
    10021 gtgaaggtga acctaaaggt gcaccgaaca cctatcttat tgatggcctg actagtatag
    10081 gttatcacca cgatgggctt catattggtt atcctggcgg tgaacctgat tggcattatg
    10141 ttaaaaatct agaaggtatc actcctcaaa atcttcttaa atctttctct aaaaaaggac
    10201 gccccttggt taagaaagcc atgtcttttg gtataaaaat acgtgtcctc aagcgggaag
    10261 aactacatat ctttaaggat attacttctt ctacttctga tcgccgggac tatatggata
    10321 aatctctaga ttattatcaa gatttttatg atagtttcgg agataaggcg gaatttgtca
    10381 ttgcaactct aaactttagg gaatatgatc ataacctaca attaaatgca aagaaattgg
    10441 aagaacagat tacagtcctt gataatcgcc atcaaaataa tacagattct gccaaatacc
    10501 atcgtcaacg tactgagctt gtcaatcaac tagctagttt agataaacgt cgcaaagaag
    10561 tcgaaccttt tattcaaaaa tttggaaatc aagatgttgt tttagctggt agcctcttta
    10621 tttatagccc taaagaaact gtctatcttt tctctggctc atacacagaa tttaataaat
    10681 tttatgcgcc tgcagttctt caagaatatg ttatgcaaga ggctctaaag cgtcaatcaa
    10741 ctttctataa ttttttaggt attcaaggaa attttgatgg ttcagatggc gtacttcgct
    10801 ttaaacagaa ttttaatggt tacattgttc gtaaaatggg tactttccgc tactatccaa
    10861 atccattaaa atataagagt attcaattac taaagaaaat cttacgccga acataaaaaa
    10921 taagcttaca taagcttatt tttttatgca ttatagaaag caacatttat ttctttacta
    10981 ttattgttta cacaaataat aacgttcgtt atttagcgaa attttattac caatatcatt
    11041 attttcttgc cgctctgtta atcaagtgat aaaatagtaa aaatttatct tcaggaggat
    11101 attatggtat cgctaaaaac ggaaattgct ggttttagtt ttgacaattg tttgatgaat
    11161 gcagcgggta tttattgtat gactaaggaa gagttattag ctattgaaaa ttctgaagct
    11221 ggttcctttg ttacaaaaac aggtaccctt aaagctagag agggaaaccc tcaaccacgt
    11281 tatgcagata ctgactgggg atcaattaat tctatgggac tccctaataa aggtattgac
    11341 tattatcttg atttcgtgac tgaattacaa gaccaagaca atagcaaaaa tcacgtttta
    11401 tcattagtag gtttatcacc agaagagaca catattattc ttaaaaaggt tgaaaacagt
    11461 agttacaatg gcctgataga gttaaattta tcgtgcccaa atgttcctgg aaaacctcaa
    11521 attgcttatg attttgaaat gactgacctc atcctttcag aaatctttag ctattatcaa
    11581 aagccattgg gcatcaaatt accaccttac tttgatatct ttcactttga tcaagcagct
    11641 acaattttta acaaatatcc tttagctttt attaactgtg ttaattctat cggaaatgga
    11701 cttgttattg atgatgaaac tgtcgttata aagcctaaaa atggctttgg aggtatcggc
    11761 ggtgatttta tcaagcctac cgccttagct aacgtccatg ctttctacaa acgtttaaat
    11821 ccttcaatta aaattatcgg aacaggtggt gttaaaaacg gccgtgatgc ctttgaacac
    11881 attctatgtg gagctagtat ggtccaaatc ggaaccgctt tacaaaaaga aggaccagaa
    11941 atttttcagc gtgtaagcag agagttaaaa gaaataatgg ctgataaagg ctatcaatca
    12001 ttggaggatt ttcgaggaca acttaattac ctttaaaaat aataaaagag caaaccttgt
    12061 ttgctctttt atattgattt taccaatctt tatcaaattc acgttcttca cgttcctc
//
LOCUS       DATROY010000056        12044 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.057, whole genome shotgun sequence.
ACCESSION   DATROY010000056 DATROY010000000
VERSION     DATROY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..201
                     /locus_tag="VBD11_001161"
     CDS             <1..201
                     /locus_tag="VBD11_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6954118.1"
                     /translation="NIYTNTGLMPGPVDSPSISAIEATIKPASTDYLYFVADVKTGNV
                     YYAKDFETHKANVEKYINSQIN"
     gene            274..756
                     /gene="greA"
                     /locus_tag="VBD11_001162"
     CDS             274..756
                     /gene="greA"
                     /locus_tag="VBD11_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6954119.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            860..2320
                     /locus_tag="VBD11_001163"
     CDS             860..2320
                     /locus_tag="VBD11_001163"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6954120.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            2481..3338
                     /locus_tag="VBD11_001164"
     CDS             2481..3338
                     /locus_tag="VBD11_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6954121.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            3362..4027
                     /locus_tag="VBD11_001165"
     CDS             3362..4027
                     /locus_tag="VBD11_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6954122.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(4227..5159)
                     /gene="yidC"
                     /locus_tag="VBD11_001166"
     CDS             complement(4227..5159)
                     /gene="yidC"
                     /locus_tag="VBD11_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6954123.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(5244..5522)
                     /locus_tag="VBD11_001167"
     CDS             complement(5244..5522)
                     /locus_tag="VBD11_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6954124.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            5554..6297
                     /locus_tag="VBD11_001168"
     CDS             5554..6297
                     /locus_tag="VBD11_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6954125.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            6335..6838
                     /locus_tag="VBD11_001169"
     CDS             6335..6838
                     /locus_tag="VBD11_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6954126.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            6861..7550
                     /locus_tag="VBD11_001170"
     CDS             6861..7550
                     /locus_tag="VBD11_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6954127.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            7618..8139
                     /locus_tag="VBD11_001171"
     CDS             7618..8139
                     /locus_tag="VBD11_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6954128.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            8223..8744
                     /locus_tag="VBD11_001172"
     CDS             8223..8744
                     /locus_tag="VBD11_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954129.1"
                     /translation="MVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVPLFLSIFI
                     SVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLMSKWQTLL
                     FAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFYIALFIWK
                     WMTQKLGKTLRIR"
     gene            8902..9141
                     /locus_tag="VBD11_001173"
     CDS             8902..9141
                     /locus_tag="VBD11_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6954130.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            9316..10110
                     /gene="racE"
                     /locus_tag="VBD11_001174"
     CDS             9316..10110
                     /gene="racE"
                     /locus_tag="VBD11_001174"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6954131.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            10107..11081
                     /locus_tag="VBD11_001175"
     CDS             10107..11081
                     /locus_tag="VBD11_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6954132.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            11063..11584
                     /locus_tag="VBD11_001176"
     CDS             11063..11584
                     /locus_tag="VBD11_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6954133.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            11581..>12044
                     /locus_tag="VBD11_001177"
     CDS             11581..>12044
                     /locus_tag="VBD11_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6954134.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKN"
BASE COUNT         3934 a         1895 c         2368 g         3847 t
ORIGIN      
        1 aacatctata ctaatactgg tttgatgcct ggaccagttg atagtccaag tatctcagca
       61 attgaagcga ctataaaacc tgcttcaaca gattaccttt attttgtcgc tgatgtgaag
      121 accggaaatg tatactacgc taaagatttt gaaacacaca aagctaacgt tgaaaaatat
      181 atcaatagtc aaattaacta agcttataaa catggtgcga gtcatcatgt ttatatcttt
      241 gacttgaaaa aatgaaaaga aaagagaaat aaaatggcag aaaaaactta cccaatgaca
      301 caagtggaaa aagatcaact tgaaaaagaa cttgaagagt taaaattagt tcgtagacca
      361 gaagttgtag agcgtatcaa gattgctcgt tcttatggtg acttatcaga aaatagcgaa
      421 tatgatgcag caaaagacga acaagcattt gttgagggtc aaattcaaat tttagaaaca
      481 aaaattcgtt atgctgaaat tattgatagt gatgcagttg ctaaagatga ggttgcaatt
      541 ggtaaaactg tccttgtcca agaagttggt acaaatgata aagatactta ccatatcgtt
      601 ggtgctgctg gagctgacat tttttcagga aaaatttcga atgaaagccc aattgctcat
      661 gctttaattg gtaaaaaaac aggagactta gctacaattg aatcacctgc tggtagctat
      721 caagttgaaa tcattagtgt tgaaaaaaca aattaatagt ataaatcata gaataatatc
      781 tagagtctag acgttattct atttttttta agtatttttt gttatcttat aataaaaata
      841 cattttatga ggtgtttata tggtttttaa agatgctacc gctatggtac aagcgataaa
      901 acagcataag atttcgtcgc aagaattagt agagcaagca atctacaaga tcgaggaaca
      961 gaatgtaagc gttaatgctg ttgtcagcaa gcaatataat gaggcgcgtc aagcagccaa
     1021 atacgcaaat gaatcaaatg ctccttttgc aggagttcct attcttctga aagatttggg
     1081 acagaatcaa aaaggacaac tctcaacctc aggttcacaa ttatttaaac actatcatgc
     1141 taaacaaacg gactatttag tgcagtcctt tgaaaaatta gggtttatta ttttaggaag
     1201 aacaaatacc cctgaatttg gttttaagaa tattagtgat ggtcaacttc acgggaatgt
     1261 taatcttcca tttgaccatt caagaaatgc tggaggctct agtggtggtg cagccgcagc
     1321 tgttagttca ggaatggttc caattgcagg agctagtgat ggaggagggt caatcagaat
     1381 tccagcaagt tttaatggtt tgattggttt aaaaccgagt cgaggacgta taccagttgg
     1441 accttcatcc tatagaggtt ggcaaggggc ttctagtcat tttgctttaa caaagtcagt
     1501 tagagataca aaacgactat tatattattt acaatcatat caagtagaaa gcccatttcc
     1561 cctgaaaaaa ctcagcaaag agagtctttt cgagtttagc gttagtaaac ccctaaaaat
     1621 agcagtattg atggattctc ccttaaaaac aaaggtatca agtgaggcta aagcagctat
     1681 taaagaagcc gcagattttc tgtctcaaaa agggaatcat ttagaattag tagaacaacc
     1741 tttagatggt attcattcta tgaaaactta ttgtatgatg aatagcgttg aaacagcagc
     1801 aatgtttgat gacatcgaaa agagtttagg gcgttcaatg gaatttagcg atatggaatt
     1861 aatgacctgg gctatgtatc agagtggaca aagggtattg gctaaagatt acagtaaact
     1921 cttagattca tgggatcaat ttgcagctac catggcgagg tttcatgaaa attatgattt
     1981 aattttaaca gcagcaacta atcaaccagc tccttttcat ggacaatttg atttagacga
     2041 gacactccag aaacaattga ggcatatggg agaattctca gtaagtgaac aacaggattt
     2101 aatttggaaa atgtttgaag actcaatggc ctggacacct tttacacatc agccaaactt
     2161 gacagggcaa ccttctttag ccataccaac tcatctaact aaagaggggc tacctttagg
     2221 tgtacaattg acagcggcta aagggagaga agatttattg cttgctgttg cagagttgtt
     2281 tgaaaaagag aagcaattca aagggcctgt ttaccattaa tttgaaaagt aaacaatatt
     2341 tttgcgaaaa cggatacaat atggtactct taacaatggt tgtattatta tcgtaaattt
     2401 atgtattaat tttcattaaa tttaattttc gatagagtac gccaaaccat tttaatataa
     2461 tttaatctac aggagtactt atgaaaaaga caattttact tggtttagtc ggtttatctg
     2521 caatgacatt agcagcttgc tcaaatggtc aatctaacaa agaaacaaca tgggacaata
     2581 ttaaaaaaga tggtgtcctt aaggttgcta cccctgcaac gctttatcca acaagttatt
     2641 atgatgacca taaaaaattg actggttatg agattgatat gatgaaagcg attgctaaaa
     2701 aacttaagat aaaagttaag tttgttgaag ttggagttgc tgaatcattt acttctgtag
     2761 atagtggtaa ggtggatgtt gctgtcaata actttgacac aactcccgag cgtttgaaaa
     2821 aatacaattt ctctcaacct tataaatatt ctgttggagg tatgattgta cgtgcagatg
     2881 gttcttcaaa gattacagca aaagatttat ctgactggaa aggtaaaaaa gctggtggtg
     2941 gcgctggaac gcaatatatg aaaattgcaa aacagcaagg tgctgaacct gttatttatg
     3001 acaatgtaac taatgacgtt taccttcgag atgtatcaac tggacgtaca gatttcattc
     3061 cgaatgatta ctacacacaa gtcattgctg taaaatatgt aacaaaacaa tatccagata
     3121 tcaaagtaaa aatgggtgat gtgaagtata atccgactga gcaaggtatt gtgatgagta
     3181 aaaaagataa gagcctcaaa acaaaaattg atgcagctat caaggatatg aagaaagacg
     3241 gttctttgaa aaaaatctct gaaaaatatt atgctggtca agatctcaca aaagaacctg
     3301 ctggaacgga taaaattcca gtaattaaag ttaaataaaa aatttcgaga ggagtagata
     3361 agtgataaac tgggatgcca tttttaattt ggaactagct gttaaggctt ttccaagtgt
     3421 tattcagggg ttaccatata ccattggatt atccttggtt ggctttattt taggagctat
     3481 cgtaggattc tttgtagctc taatgaagat gtctcatttc cgcttactga ggtatctagc
     3541 taatattcat atttccttaa tgcgtgggat tcctttaatg gttctactct ttcttatcta
     3601 ctttggtttg ccttttatag ggatacagtt agatgctgtg actgcttcta tcgtaggttt
     3661 taccatgatg tctagtgcat atatatcaga aattatcaga gcagctcttt tagctgttga
     3721 tcatggtcag tgggaagctg ctagagcttt agggttaaaa acaccgacga tttatagagg
     3781 cattattatt ccgcaagcaa caagaattgc actcccttct ttaagtaatg ttttgctaga
     3841 tatggtcaag ggttcctcat tgactgcgat gattacggta cctgatatct ttaataatgc
     3901 taaaatagtt gggggaacct actccgacta tatgaccgcc tatatcttag tagcccttat
     3961 ctattgggta atttgtacgc tttatgcaat tattcaagac tggtgggaaa aacgattact
     4021 ttattaataa ggtagtctgt tagctagatt acttgcactt taaaagctag atttttatct
     4081 gacttttagg gtgcttttta tatgaaaagt taggcaaaac taaaaagact tagctatgta
     4141 ggacatagaa ctaagtcttt tttacttgaa ttacataata atctttaact gtttaggaca
     4201 acacaccatt atttagtatg cttgttttat ccccgtcttt tttgttttcc ggcgttacga
     4261 tttgtttttt tagatgtaat aattgcttga ttagattcag tggctttagt tgaatttgta
     4321 acatcttttc gtgcgttatt agctttgtag gctttaggag gatttttagt atattcttct
     4381 tctacttttc ggcgtagttt aggcttaagt acataagtcg taactagctg ctggataata
     4441 ctgaaaatac caccaataaa ccaataaagc gcaacactag caggtaaact aatagacatg
     4501 aataccatca ttattggcat cagatacatc attgttttca tttgctgacg ttgttcatct
     4561 ggaacacctt gcattgacaa ccaagattgt acaaaataaa gaatggcgat aatgacagta
     4621 agtatcagac tcttttgacc taagttaagt cccaagaagg ttgcactaga gactccagga
     4681 gtatagcgtg cagcaaagaa aatagctgag aaaaatggca tctgaatgag taatggcaag
     4741 cagccaatac cgccaaacat gctaaggcca ttctcacgtt gagctgtcat taattctgtc
     4801 tgagctgcta atttttcttc ttgtgtttta gcattgcgta gtcgttcatt aataggttca
     4861 aataaaggct taaagtacgc cattttttca gcttgatagc tagctttcca agactgataa
     4921 agtccaagtg gtaagataac gacacgtacg atgacggtta caataatgat agcgacacca
     4981 aaaccaagcc cttggtgttg agcgaaataa gtaatgaggt tggccatagg cactccaaga
     5041 gtattccaga taactccata gggttttcca gccttatcaa cgctaacaca tcctgtcaat
     5101 aataataaca tagacaaaga taagctagag aataagatac gtttcaatgt ttttttcaat
     5161 gtttttagtt cctctaattt ttaatatacc tatctatttt actttttttt taagaattaa
     5221 acaatacttc cgcagaatga tttttaatta ctcattttaa agtcattaaa atcatcgaag
     5281 ttatctagtt taatatcaac ataagttact ttagaccatt ttgaaggtcc ttttcgaatt
     5341 ttctgaataa attgtgtcat tttatttgaa tctgtggatt gtgctaaaat ttcaacagtt
     5401 ccatcatcgt tattccaaac gcgaccgtaa atatcaccta tttctaaagc taaggaataa
     5461 gttgcatagc gaaatccgac tccttgaacg cgccctgaga caattaaatg aacttttttc
     5521 ataacttgac ctcttttctg atataatagg tttatgacta ctattataac ttcaaaatca
     5581 aataatctca ttaaaaaaac gaaaaagttg ttacaaaaaa aatatcggaa atcttcatat
     5641 cttattgaag gctggcattt atttgaggaa gctgaaaaat atggtgcaca atttttaaat
     5701 atttttgtga cagaaacggc aatagatcgt ttgagaaaac ccgagagagc tattgttgtt
     5761 acagatgatg ttttgaaaga attaactgac tcacagactc ctcaagggat tgtagctgag
     5821 attgcctttc aagagacaag atggacagat attaaaaaag gacgttttct tgtattagaa
     5881 gatgtgcagg atccaggaaa cctaggaaca atggttcgaa ctgcagatgc agcgaatttt
     5941 gatgctgttt ttttatctca gaaatctgct gatttgtata accaaaaaac acttcgttct
     6001 atgcaaggca gtcactttca tttaccagta tttagggtag aaatagagca gtttgttaac
     6061 ttttgtaaag cagaagggat aactatgatt gcgacaactt tatctgaaca atcggtaaat
     6121 tataagaatc tccctaaata tgattatttt gctttaatca tgggaaatga gggacaggga
     6181 atttccaaaa ctatgacaga agaagcagat gttttagcac atattgaaat gcctggacaa
     6241 gcagaaagtc ttaatgtagc agtagctgcg ggagtagtca ttttcagctt aatttaagtt
     6301 catatgttat aatatcccca gaggggtgat tataatgaaa aagtatcaag acgataagga
     6361 cttcatggat ttggtcggtc accttattga ccatccacgt ttccaaaaac tcgaagctat
     6421 tgtccaacat catcactcaa ctcgcttaga gcattctata aatgttagtt atacaagtta
     6481 taaaattgct aagaagtttg gttgggatgc gagtagtact gctcgaggtg gtttactaca
     6541 tgatttcttt tattatgatt ggcgtgtaac gaaatttaac aagagccatg cctgggttca
     6601 tccaaggata gcggttcgta atgcgcgaaa attaactgat ttaaacgcta gagaagaaga
     6661 tattattttg aagcacatgt ggggagcgac tattgcaccg ccacgttata aagaaagtta
     6721 tattgtgact atggttgata agtattgggc agtacgagag gcttctcgtc cactcaaacg
     6781 tatttttaag aaacctatcc gttttagtcg caaattctta ggtagtcata atcattaaaa
     6841 tttagaatag gaaaatacat atgaatgata acgtcattta cacacaatct gattcagggc
     6901 tgaatcaatt ttttgctaag atttacggtc ttgtagggat aggagtaggt ttgtcagctg
     6961 ctgtttctgc gattatgctg tatatgtttc cacaaaatat gattgccatc atgcaaaaaa
     7021 tgccagggtt gtattttgga gcaatcatct tagaattggt tttagtcttt gttgctagtg
     7081 gagcggcgcg tcgaaacaca ccagctgcat taccattatt tttgatttat tcagctttga
     7141 atgggtttac tttaagtttc attatcgcaa ggtacacgca aactacagtt cttcaagctt
     7201 ttataacatc tgcggctgtt ttctttgcaa tggctttgat tggagctaag actaaaaaag
     7261 atttatcagg tatgcgtaaa gcgttaatgg ctgctttaat tgggatttta attgcaagtc
     7321 ttgttaattt atttatcggt agtgggggta tgagttatat cattagtatc gtatgtgtca
     7381 tcatcttttc tggattgatt gcttacgata accaaatgat taaatatgtc tacaatagcc
     7441 aaggcggcca agtcgcagat ggttgggcag tttcgatggc actaagcctt tatcttgatt
     7501 ttattaatct attcttaaat attcttcgtc tttttgcacg aaatgattga ggctaactgg
     7561 ttagatattc tagccagttt ttaattgtga aataacacca aagaaggaat ttgttatatg
     7621 aaggcacagg agcgtcgaga aaatatttta acgactttaa aaggaacaaa agaagctata
     7681 tcggcaagca cattagcaaa gatttttagt gttagtaggc aggtgattgt tggagatatt
     7741 gccttattgc gtgcacaaca atgtgatatt atttctaccc caaaaggata ccttatgtcc
     7801 agtgctctaa gtactcatca attcacagct agattggttt gtcagcatgg catagaacag
     7861 acagaagaag aactggaaat tattttgcgg tatcaaggaa tcattatgaa tgtggaggta
     7921 gagcacccta tttatgggat gctgactgct cccttaaaca ttcaaagcca aaaagacatt
     7981 gataatttta ctgctaaatt aaaagtttcc aatgcagaat tgctatcctc tttgacagat
     8041 ggtctacata cacatatgat ttcttgtcaa gatcagtcag tttttgacca aatatgtgag
     8101 gctctaaaaa aagcaggaat tttatacata gacaaataac cttacaggtg tctttacacc
     8161 tgtaaggtta ttttgtataa tagcttcaaa ggagataaaa atgtcaaaaa aaaacaacac
     8221 aaatggtttc ttatacttct attttagtag cttttgcaat catgattcct ataatcatgc
     8281 ctgctaaaat aattattggg cctgcttctt tcaccttagc aagccatgta cctttatttt
     8341 taagtatttt tatatctgtt ccagttgcaa tattggtagc tttagggaca ggacttggat
     8401 ttctactagc tggttttccg attgtaatcg ttcttagagc tttatcacat atcggatttg
     8461 cattaattgc ggctttcttg attaagtcaa agccctcttt attgatgagt aaatggcaaa
     8521 cattgttatt tgctgtagct attaacataa ttcatggtct gctcgaattt attactgttt
     8581 atataatcac aatgacttct aacagtagtt caacttattt atggtcacta ttttctttga
     8641 taggactagg ctctttgcta catggattgg tagattttta catagctctc ttcatttgga
     8701 aatggatgac acaaaagttg gggaaaacac ttaggataag ataaaggact cgactatgtg
     8761 cttaaccatg ctctagtgaa aatttaataa tgtattgacg tgacaaggac gatagaggct
     8821 ttgattttgt ttacatatat ttgtaaactc tttctttttt tgctataatg agtcatgtaa
     8881 ataaatgagg agaatgaata gatgtcaaca actatttgga ttttattaat tatcgtcgct
     8941 ttgtttggtg gtctcgtggg aggcattttt attgctcgta aacaaattga aaaagagatt
     9001 ggagagcacc ctcgtttaac accggatgct attcgtgaaa tgatgagtca aatgggacaa
     9061 aaacctagtg aagctaaagt tcaacaaaca taccgtaata ttgtaaaaca cgcaaaaaca
     9121 gctatcaaaa ctaaaaaata agagtgtttt agcattgaag cgtttagtga tgaaatagtg
     9181 agtgatagtc catcagattt ctagacaaga tttgggcaaa cttggtcaag tgtagatttg
     9241 actgattggt tattgtatag agaggctagg actgttgtcc agcctctttt agtcactaat
     9301 attggaggaa taaaaatgga tagtaggcct attggctttc tagattctgg tgtaggtggt
     9361 ttaacggttg ttaaggaaat gttccgtcaa cttccagaag aggaagtaat cttcattgga
     9421 gatcaggcta gagctccgta tggtcctaga cctgctcaac agattagaga gtttacctgg
     9481 cagatggtta acttcttatt gactaaaaat gttaagatga ttgttatagc ttgtaataca
     9541 gcaactgcag ttgcctggca agaaattaaa gaaaaactag acatccctgt tttaggcgtt
     9601 attttaccag gagctagcgc agctatcaaa tcaactaatt tagggaaagt tggtattata
     9661 ggtactccca tgactgttaa atcagatgct tatcgtcaaa aaattcaagc tttgtctcca
     9721 aatactgctg tggtatccct tgcttgtccg aaatttgttc caattgtgga atcaaatcag
     9781 atgtcttcta gtttagccaa aaaggtggtt tatgaaacgt tgtccccatt agttggtaaa
     9841 ttagatactt taattttagg ttgcacgcat tatcccctat tacgtcccat cattcaaaat
     9901 gttatggggg ctgaggttaa attaattgat agtggcgcag aaaccgttcg tgatatttct
     9961 gttttattga actattttga gataaaccat aattggcaaa ataaacacgg tggtcatcac
    10021 ttttacacaa ccgccagccc aaaaggtttt aaagaaattg cagaacaatg gcttagtcaa
    10081 gaaataaatg tggagcgtat cgtattatga caaaaacaat ttttgaaagt aaaactgaag
    10141 gcaattggtt tttaggtagt ttccaagctt ttaattattt tacttgtttt ggtaatgatg
    10201 aatcttacga agctatccaa gatgtttttc atcgtctatt atcaactttg aaagttgaag
    10261 gtctacagct tcacgttgtg cagatgactt cggattttca attattagca ttcctcgttg
    10321 atatgattaa tcaagagtat tcaaggcata tcaaagtgac tcaacataaa ggggccatct
    10381 tagttagtga agatgatcag ttgttcttag ttcacttacc taaagagggc acttctttag
    10441 aaaaattctt tgatttaaag aatgataata attttgggga taccatatta attgcgactc
    10501 acaatgaagg aaaaacgaag gaatttagag aattatttgg gaaattagga ctcaaagttg
    10561 aaaatttaaa tgattaccca gatttaccag aagttgaaga gactggtatg acctttgaag
    10621 aaaatgctcg acttaaagca gagactattt caaaattaac tggaaaaatg gtaatttcag
    10681 atgattccgg attaaaagta gatgctttag gtggtttgcc aggggtatgg tctgctcgtt
    10741 tttcaggtcc tgacgcgaca gatgctcgta ataatgccaa attattgcat gaattagcca
    10801 tggtatttga caaagaaaga cgttcagcac agtttcatac aaccttagtc gtgtcagctc
    10861 caaataaaga aagtttggta gttgaagcag agtggccagg ttatattggt acggaaccta
    10921 agggggaaaa tggctttggt tatgatccgc tctttattgt cggagaaggt agtaggacag
    10981 cagctgaatt gagcgcacaa gaaaaaaata atctatcaca tcgtgggcaa gctgtccgta
    11041 aattgatgga ggtatttcca aaatggcaat tagaaaatta gttgtaatga gtgattctca
    11101 cggagatcgt gacattgtaa aagatataaa gaatcattat ttgggaaaag tggatgctat
    11161 ttttcacaat ggggattccg aacttcctag ctcagatcct atatgggaag gcattcatgt
    11221 ggtcacagga aattgtgatt atgattcagg ttatccggaa gttttggtta caaaaataga
    11281 taatgctgtt attgttcaaa cccatggtca tctccatcag attaatttta cctgggataa
    11341 attggattta ttagctcagc aagaagacgc ggatatttgt ttatatggtc atcttcatcg
    11401 tgcagatgct tggaaaaatg gtaaaactat ttttatcaat ccaggtagtg ttttgcagcc
    11461 aagaggacca ataaatgaga agctttacgc tgttgtaact ataacagata gtaaggtttt
    11521 ggtagagtat tatactcgcc aacaccaacc ctatcctaac ttgacaaagg aattttcaag
    11581 atgattgcta aagaatttga gtcgtttttg ctaaatcatt tggaccacta tctaattcca
    11641 gctgaagacg ttgctatttt tgtggataca cacaatgcag accacgttat gttactatta
    11701 gcaagtaatg gtttttcacg tgtaccagtt atcactaaag agaaaaaata tgtaggtact
    11761 attagtatct cagacattat gggctaccaa tctaaaggtc aattaacaga ttgggagatg
    11821 gcacagactg atattgttga gatggttaat acaaaaatcg agccgattaa tgaggcagct
    11881 actctgacag caattatgca taagattgta gattatccat ttttacctgt tatcagtgat
    11941 caaaatgatt ttagaggcat tataacccgt aaatcgattt taaaagctat taatagcctc
    12001 ctacatgatt ttacagacga atatactatc actcctaaaa acaa
//
LOCUS       DATROY010000057        11881 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.058, whole genome shotgun sequence.
ACCESSION   DATROY010000057 DATROY010000000
VERSION     DATROY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            246..317
                     /locus_tag="VBD11_001178"
     tRNA            246..317
                     /locus_tag="VBD11_001178"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Gln,seq:ttg)
     gene            326..397
                     /locus_tag="VBD11_001179"
     tRNA            326..397
                     /locus_tag="VBD11_001179"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:359..361,aa:Glu,seq:ttc)
     gene            460..978
                     /gene="rimM"
                     /locus_tag="VBD11_001180"
     CDS             460..978
                     /gene="rimM"
                     /locus_tag="VBD11_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6954135.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            965..1720
                     /gene="trmD"
                     /locus_tag="VBD11_001181"
     CDS             965..1720
                     /gene="trmD"
                     /locus_tag="VBD11_001181"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6954136.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1701..2693
                     /locus_tag="VBD11_001182"
     CDS             1701..2693
                     /locus_tag="VBD11_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6954137.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2830..3900
                     /locus_tag="VBD11_001183"
     CDS             2830..3900
                     /locus_tag="VBD11_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6954138.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3913..4836
                     /locus_tag="VBD11_001184"
     CDS             3913..4836
                     /locus_tag="VBD11_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6954139.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            5048..6682
                     /locus_tag="VBD11_001185"
     CDS             5048..6682
                     /locus_tag="VBD11_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6954140.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6826..7911
                     /locus_tag="VBD11_001186"
     CDS             6826..7911
                     /locus_tag="VBD11_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6954141.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            7936..8061
                     /locus_tag="VBD11_001187"
     CDS             7936..8061
                     /locus_tag="VBD11_001187"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO6954142.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            8126..8935
                     /locus_tag="VBD11_001188"
     CDS             8126..8935
                     /locus_tag="VBD11_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6954143.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8932..9843
                     /gene="pfkB"
                     /locus_tag="VBD11_001189"
     CDS             8932..9843
                     /gene="pfkB"
                     /locus_tag="VBD11_001189"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6954144.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9840..11804
                     /locus_tag="VBD11_001190"
     CDS             9840..11804
                     /locus_tag="VBD11_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6954145.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
BASE COUNT         3961 a         1773 c         2429 g         3718 t
ORIGIN      
        1 ggtgtggcta tacaagatat attagaaatt gggatgaata ataagcagta ccatgaacta
       61 cttttacaag ctattatcga aaaaagggac caaaaagttt ttgaatatat ttcaaaaatg
      121 aaacataaaa tactttgata gactctatat gagctttatt aaaagggata aaatcgaaat
      181 ttaaaattta attcgaaaag atattgacag taatctagaa tttcgatata ctagattttg
      241 tcttttgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
      301 gaatccagct accccagttt cacatggtcc gttggtcaag gggttaagac accgcctttt
      361 cacggcggta acacgggttc gaatcccgta cggactatcg tggccagttg cattttagcg
      421 gctggttttt cttttatttt caaaaatatc ggagttacta tggaatactt taacgttgga
      481 aaaattgtca atacacaagg attacaaggc gaaatgcgtg tattaagcgt gactgacttt
      541 gtagaagaac gctttaaaaa aggtcaagtg ttagctttat tcgatgaaaa gaatcagttt
      601 gttatggata ttgaaattgc tagtcatcgt aagcagaaga attttgacat tataaaattc
      661 aaaggaatgt atcatattaa tgatattgaa aaatataaag gtttcacact gaaggtggca
      721 gaggatcaac tgagcgacct taaagatggt gaattttatt atcatgaaat tattggctta
      781 gatgtctatg aaggggaaga gctgattgga aaaatcaaag aaattttaca accaggagca
      841 aatgacgttt gggttgtgga acgtcatgga aaacgtgatc ttttattacc ctatatcccg
      901 ccagtagtat tagaagtgga cctttctaat cagcgtgttc aagttgaatt gatggaaggt
      961 ttagatgatg aagattgata ttttaacttt atttcctgaa atgtttgccc ctctggaaca
     1021 ttctatagtt ggtaaggcaa aagaaagagg acttcttgaa attaattatc acaatttcag
     1081 agaaaatgct gagaagtctc gacatgttga tgatgagcct tacggtgggg ggcaagggat
     1141 gcttttacgt gctcaaccta tttttgatac aattgataaa atagatgctc aaaaagcacg
     1201 tgttattttg ctagacccag cgggcagaac atttgatcag gattttgcag aagaactatc
     1261 caaagaagat gaattaattt ttatttgtgg tcactatgaa ggttatgatg aacgtataaa
     1321 atcattagta acagacgaag tatctttggg agattttgtt cttacgggag gagaattggc
     1381 ggctatgaca atggtcgatg ctaccgttcg tctgattcca gaagttatag gtaaagaaac
     1441 aagtcatcag gatgattcat tttcatcggg cctattagaa tatcctcagt atacgcgccc
     1501 atatgattat ttaggcatga cagttcctga tgttctcatg agtggtcatc atgagaacat
     1561 ccgaaaatgg cgtttagaac aaagtttgcg aaaaacacta gaacgtcgac cagacttgtt
     1621 agaaaattat gcaatgacgg atgaagagag acttatctta gagaaaatta aaacagaaat
     1681 agaaagaaca gatacagtaa atgaacaaaa caatttatga tatcactatt gtaggaggcg
     1741 gaccagtagg tctctttgct gctttttatg ctggactacg cggagtatcc gttaaaatta
     1801 ttgaaagcct ttcggaatta ggaggccagc cagctatcct ttatcctgag aaaaaaatct
     1861 atgatattcc tggttatccg gtgattactg gacgtgaatt aattgataaa catattgaac
     1921 aattagagag atttaaagac tctattgaaa tctgtttaaa agaagaagtt ttatcatttg
     1981 aaaaagtaga tgatgttttc acaattcaaa cagataaaga tcaacatttg tcacgtgcta
     2041 ttgtttttgc ttgtggcaac ggtgcttttg ctccgcgttt actaggtttg gaaaatgaag
     2101 aaaattatgc agataataat ttattctata acgttacaaa gttagaacag tttgcaggta
     2161 aacatgttgt tatttgtggt ggaggcgact cagctgttga ttgggccaat gaattagata
     2221 agattgcagc tagtgtagcg attgttcatc gtagagatgc ttttagagcg catgagcata
     2281 gtgtggatat actaaaggca tcaggtgtta gaattttaac cccttatgtc ccaattggtt
     2341 taaatggaga tagtcagaga gttagcagct tggtcgttca aaaagttaaa ggtgatgaag
     2401 taattgaatt acctttagac aacttgattg ttagttttgg cttttctact tctaataaaa
     2461 atttacgtta ttggaatttg gattataaac ggtctagtat caatgtttct tcactttttg
     2521 agacgactca agaaggcgtg tatgctattg gagatgctgc taattatcca ggaaaggtag
     2581 aattgattgc aacaggatat ggggaagcac cagtcgctat aaaccaagct atcaattata
     2641 tttatcctga tcgagataac cgtgtagtcc attcaacatc attgataaaa taactataaa
     2701 taaaactgat aagtttataa aaaaggatta tatatattgt taaaaaaata acaaaacttc
     2761 ctatttattt tgtgatataa ttaactttgt cgttatgaat gatattaaat aaatgaagcg
     2821 aggtatttta tgacaaatac ggtaacacca aaagagacgg ctggctcttt tatcaataaa
     2881 gtgttaggtg ggacagcaac tgcaatcgtt gtagcactca tcccaaatgc tatcctagcc
     2941 acttttttga aaccattttt atcttacgga cttgctgcag agttcttaca catcgtgcag
     3001 gtattccaat ttttcacacc aattatggca ggatttttga ttggtcaaca gtttaaattc
     3061 accccaatgc aacagcttgc tgttggaggt gctgcatata ttggttctgg tgcgtgggct
     3121 tacacagaag ttattcaaaa aggagttgct actggtagtt tccaattgcg tgggattggt
     3181 gatctcatca atatgatgct aacagcagct ttagcagttt tagcagttaa gtggtttggt
     3241 aataaatttg gttccctaac cattatcttg ttacctatta tcataggtac aggcgtaggc
     3301 tatttaggat ggaaattact cccttacgtt tcatatgtga caacacttat tggtcaagga
     3361 attaattctt ttactacttt acaaccaatt gcgatgtcaa ttctaattgc gatggctttt
     3421 tcaatgctta ttgtaagtcc tatttcaaca gtagcgattg gtctagcgat tggtcttaat
     3481 ggtatgtcag caagtgcagc ttcaatggga gttgcttcaa caacagcagt gctagtttgg
     3541 gcaacgatga aggctaataa gtctggagtt ccaattgcta ttgctttagg tgcaatgaag
     3601 atgatgatgc caaatttcct aaagcatcca gtaatggcga ttccaatgct tatgactgct
     3661 acagtaagtt ctctaacagt cccattgttt aaattggtgg ggacaccagc ttcgtctggt
     3721 tttgggcttg ttggtgcagt aggtccgatt gcttcatttg aagcaggagc aagtatgttg
     3781 attgttatct tatcttggct cgttattcct tttgcagtag gttttgtatc acacaaaatt
     3841 tgtaaagaca ttctgaaact ttataaagac gatattttcg tttttgaagg tcaaaattaa
     3901 tttggaggtt gtatgttagt ttatattgct ggttcaggcg ctatgggatg tcgttttgga
     3961 tatcaaattt ctaaaacaaa tcatgatgtt attttacttg ataattgggc agaccatatt
     4021 atggctatta aagaaaatgg cttgaaggta acaggtgata cagaggacct tgttaaactt
     4081 cctatcatga agccaactga tgcgacagaa gaagcagatt taattatttt gtttactaaa
     4141 gcaatgcagt tgccaaatat gcttcaagat attaaaaaaa ttattggtaa gaaaacaaag
     4201 gtcttatgtc ttttaaatgg attaggacat gaagatgtca ttcgtcaata tatccctgaa
     4261 cacaatattt tgatgggagt aacagtttgg actgcagggt tgaaaggacc agggcatgct
     4321 catctggaag gagttggttc tgtaaatctt caaagtatcg atcctaacaa tcaagaagca
     4381 ggtcatcgcg ttactgaatt gcttaatgaa gcaaaattac aggctactta tgacgaaaat
     4441 gttttgccaa atatttggag aaaagcttgt gttaacggaa cgatgaattc aacttgcgcc
     4501 ttgctagatt gtacaattgg tcaactcttt gctagtgaag acggcgtaaa tatggtacat
     4561 gaaattatcc atgaatttgt tactgttgga aaagcagaag gtgtagagct tgatgaagaa
     4621 gaaataacaa agtacgttat ggatacatct gttaaagcag cccatcatta cccttcaatg
     4681 catcaagact tagttcaaaa tcagcgctta actgaaattg atttcttaaa tggcgcagtg
     4741 aataaaaaag gtgaaaacct aggcattgat acaccttact gtcgattaat cacacaatta
     4801 attcacacta aagaaaatgt acttagcata aaataataat caaccgggag aatagtgccc
     4861 ggttttttga tatgcttaat gataatgatt ttgttatact aagaataata agagttgggt
     4921 atttttttcg agaagtattt acagtataaa atattacatt tgtattacaa atatttctaa
     4981 aatatgttga catgttacaa aaaggatatt ataatagtag tgtaaataaa taaggaagta
     5041 ggtgtttatg atgaaaaaag gacaagtaaa tgatactaag caatcttact ctctacgtaa
     5101 atataaattt ggtttagcat cagtaatttt agggtcattc ataatggtca caagtcctgt
     5161 ttttgcggat caaactacat cggttcaagt taataatcag acaggcacta gtgtggatgc
     5221 taataattct tccaatgaga caagtgcgtc aagtgtgatt acttccaata atgatagtgt
     5281 tcaagcgtct gataaagttg taaatagtca aaatacggca acaaaggaca ttactactcc
     5341 tttagtagag acaaagccaa tggtggaaaa aacattacct gaacaaggga attatgttta
     5401 tagcaaagaa accgaggtga aaaatacacc ttcaaaatca gccccagtag ctttctatgc
     5461 aaagaaaggt gataaagttt tctatgacca agtatttaat aaagataatg tgaaatggat
     5521 ttcatataag tcttttggtg gcgtacgtcg atacgcagct attgagtcac tagatccatc
     5581 aggaggttca gagactaaag cacctactcc tgtaacaaat tcaggaagca ataatcaaga
     5641 gaaaatagca acgcaaggaa attatacatt ttcacataaa gtagaagtaa aaaatgaagc
     5701 taaggtagcg agtccaactc aatttacatt ggacaaagga gacagaattt tttacgacca
     5761 aatactaact attgaaggaa atcagtggtt atcttataaa tcattcaatg gtgttcgtcg
     5821 ttttgttttg ctaggtaaag catcttcagt agaaaaaact gaagataaag aaaaagtgtc
     5881 tcctcaacca caagcccgta ttactaaaac tggtagactg actatttcta acgaaacaac
     5941 tacaggtttt gatattttaa ttacgaatat taaagatgat aacggtatcg ctgctgttaa
     6001 ggtaccggtt tggactgaac aaggagggca agatgatatt aaatggtata cagctgtaac
     6061 tactggggat ggcaactaca aagtagctgt atcatttgct gaccataaga atgagaaggg
     6121 tctttataat attcatttat actaccaaga agctagtggg acacttgtag gtgtaacagg
     6181 aactaaagtg acagtagctg gaactaattc ttctcaagaa cctattgaaa atggtttacc
     6241 aaagactggt gtttataata ttatcggaag tactgaagta aaaaatgaag ctaaaatatc
     6301 aagtcagacc caatttactt tagaaaaagg tgacaaaata aattatgatc aagtattgac
     6361 agcagatggt taccagtgga tttcttacaa atcttatagt ggtgttcgtc gctatattcc
     6421 tgtgaaaaag ctaactacaa gtagtgaaaa agcgaaagat gaggcgacta aaccgactag
     6481 ttatcccaac ttacctaaaa caggtaccta tacatttact aaaactgtag atgtgaaaag
     6541 tcaacctaaa gtatcaagtc cagtggaatt taattttcaa aagggtgaaa aaatacatta
     6601 tgatcaagtg ttagtagtag atggtcatca gtggatttca tacaagagtt attccggtat
     6661 tcgtcgctat attgaaattt aaagctaaat ctttttagat ttagctttac taatattatg
     6721 tgtgttggaa aaaacagtgg agatttacgt caaaattata ttatttggat agtatcgaat
     6781 agatagaatg tggagaaaag taggaaatag ttaagaaagg tatttatgac gcttcgagaa
     6841 ttaacaatag aagaatttaa agaacattca ggaaattatg attcacaatc atttttacaa
     6901 acacctgaga tggctaaact tttagaaaaa cgcggctatg atgttaggta tttgggatat
     6961 caagtagaaa ataaactaga gataatcagt ttatcttata ttatgccagt cactggtggt
     7021 tttcaaatga aaattgattc aggaccagtt cattcaaatt ctaagtatct aaaacaattt
     7081 tataaagcat tgcaaggcta tgccaaatcc aacggtgttc tagaattaat agttgagcct
     7141 tttgatgatt accaattatt cactagttcg ggagttccta gtaatcaggg aaatgataat
     7201 ctgattgaag attttaccag ttcaggttat caccatgatg gtttaacaac tggttttact
     7261 ggtaaatatt tatcttggca ctatgttaaa aatttagaag gtgtcacttc tgaaacgtta
     7321 ctatcttcat tctctaagac aggacgagct ttggttaaga aagcaatgtc ttttggaatc
     7381 aaggttcgcg ttcttaaacg tgatgagcta catttattta aagagataac aacttctacg
     7441 tcaaatagac gtgattatat ggataagtcc ttagattatt atcaagattt ttacgatagc
     7501 tttgaaggca aggctgaatt tgtgattgcc actttaaatt ttagagaata cgaccataac
     7561 ttgcaaataa aagctgaagc attggaaaat aagcttaaat tgcttgatga acgttttagg
     7621 gaaaatgcag actctcctaa gtatcatagg caacgctctg aaataattaa tcaactggct
     7681 agttttgaaa cacgacgaca agaagttcag tcatttattc aaaagtatga taaccaagac
     7741 gttgttttag ctggtagtct atttgtttat agtcttaagg agacagttta tttcttttca
     7801 ggatcctaca cagaatttaa taaattttat gcaccagcag ttcttcagga atatgttatg
     7861 caagaggctc tcaaaagagg ttctactttc tataacctcc taggtattta gggaactttt
     7921 gatggttcag atagtattct tcgctttaaa cagaatttta atggttatat cattcgtaaa
     7981 atgggaactt ttaactatta tccaagtcct tttaaataca aaggaattca gcttcttaaa
     8041 aaggttttga agagaaatta aagtatttgt aaaagctaat ctttagatta ggctttttta
     8101 gtttattgat agtttttgga agaaaatgct tgacattgaa agaaaaagag atataattaa
     8161 attgcaaacg atttcaattc aaggagggac tatgctaaag tcaaagagaa aagaaattat
     8221 cttatcacga cttgagcaaa ataagagtgt tactttagat gaattaacgt ctatcttaga
     8281 gacttctgaa tctacagttc gcagggattt agatgaactt gaatctgaag gttttttaaa
     8341 aagggtgcat ggtggtgctg aattaccata ctcacttggc caggaattaa gtaatcaaga
     8401 aaaatctatc aaaaacgttc aaaaaaaatt ggatattgct agacaaactg caaagctgat
     8461 agcaaagcaa gatgttatat ttattgatgc cggaactacg acagaattat taattgattt
     8521 tctccctcac gagcaattaa cggtggtgac gaattctatt catcatgctg caaagttagt
     8581 tgatagaggg ataaaaacaa tcattatcgg aggagcggtt aaacattcaa cagatgctag
     8641 tattggccag gtagccatta atcagattag gcagataacg gttgacaaag cttttctggg
     8701 gatgaatggt atagatgaag tttatttaac tacaccagat ttagaagaag ctgctattaa
     8761 agaagctatt attaataata gtcaacaaac ctttattcta atggattcat ctaaaattgg
     8821 acaagttaca tttgctaaag tgaaagagat aaatgacatt aatttagtaa ctaataagac
     8881 tgattcagaa cttatgacaa taataaaaga gaaaatgaag gtgattcaag tatgatttat
     8941 acagtaacat taaatccatc gattgatttt attgttcgtc tagatacact attgttgggt
     9001 agtgttaacc gtatgactag cgatgataaa tatgtaggtg gtaaaggaat taatgttagt
     9061 cgcatattaa aacgcttaaa aattgacaat actgctacag gttttatcgg tggatttaca
     9121 ggacattttg ttgaagatgg acttgtatca gaaggtatca aaactgactt tgtttctgtt
     9181 aatgaagata cacgtatcaa cgttaaagta aaagcaaaaa ttgaaactga aatcaatggc
     9241 gggggtccta ggataacaaa tgaacaactt catcgtctag agaaacttct ttcgagacta
     9301 acacctgagg acacagtagt tttcgcaggt tcagcaccag ctagccttgg aaataaggtc
     9361 tataatacat tgattccaat tgctaaaaag acaggagcgg aagttgtttg cgattttgaa
     9421 ggtcagacac ttttagacgc tcttgcttat cagcctcttt tagtaaaacc gaataaccat
     9481 gaattagcag atatttttgg cgtagagcta gaaggtctgc ctgatatcga aaaatatgca
     9541 cataagattc tagataaagg agctaagaat gttatcgttt ctatggcagg ggatggtgca
     9601 ttgttagtaa ctccagaagc ttcatatttt gcaaaaccga ttaagggtga agtgaaaaat
     9661 tccgttgggg ctggtgattc aatggtagct ggctttacag gagaatttgt taagtcaaaa
     9721 aatccagtag aagcactaaa atggggcgta gcttgtggta cagcaaccac tttctcagat
     9781 gatttagcaa cagctgagtt tatccaagat atttataata aagttgaggt agaaaaatta
     9841 tgaaaatcca agacctacta aaaaaagaag ttatgattat ggatttaaaa gcaaccagta
     9901 aagaggctgc aattgatgaa atgattacaa aattagttga cacaggtgtt gtgacaaact
     9961 ttgctatttt taaagatggt atcatgaagc gtgaggcaca aacatcaact ggattaggtg
    10021 atggtatcgc tatgccacat agtaaaaatg ctgccgttaa agaagctact gtcctttttg
    10081 caaaatctgc atcaggtgtt gattacgaag cacttgatgg acagccaact gatttattct
    10141 ttatgattgc tgcgccagat ggtgctaatg atacgcattt agctgccttg gcagaactat
    10201 ctaaatatct tttaaaagaa gggttcgctg atcaattacg tcaagcaaaa acgccagatg
    10261 acattattgc tacttttgat agtaatagca tttctcaaga aacagtagct ccacaaacag
    10321 tacaatcaac aagtaaaggt tcagattata ttgttgcagt aacagcttgc actacgggaa
    10381 ttgcccacac gtatatggca gaagaagctt taaagaaaaa agcagctgaa atgggtgtag
    10441 gtattaaagt tgaaacaaat ggcgcttctg gtgtaggaaa taagttgact tcaagtgata
    10501 ttgcaagggc taagggtgtt attattgcag cagataaggc tgtagagatg gatcgttttg
    10561 atggcaaacc tttggtatca cgtccggtag ctgatggtat taaaaaaagt gaagacttaa
    10621 ttaacattat tctcgataat aaggcacaaa cctatcatgc aaaaaatcaa aatgataaac
    10681 aatcaggtga atctgatgga aaatcaggct taggctctgc tttttataaa caccttatgg
    10741 gtggtgtatc acaaatgtta ccattcgtta ttggtggtgg tattatgatt gcgattgctt
    10801 tcctttttga taacatactt ggtgtaccaa aagaccaatt aagtaattta ggttcatacc
    10861 atgaaattgc agccttattc aaaaatatcg gtggcgcagc atttgcattt atgttaccag
    10921 tattggcagg ttatatcgct tattcaatag ctgaaaaacc aggactagta gctggcttcg
    10981 tagctggttc aattgcatca agtggactgg cattcggtaa agtacctttt gcagaaggtg
    11041 ggaaagcaac cttagcatta gcaggtgtgc catcaggttt cttaggggct ttagtaggtg
    11101 gtttcttagc tggtggagtt atcctactac ttagaaaatt actttcaggt ctcccaaaat
    11161 cgcttgaagg tattaaatca attcttctat acccattgtt aggtgttttg attacaggtt
    11221 tcttgatgtt gctcgtaaac attccaatgg cagctatcaa cacagctctt aacactttct
    11281 tacaaggttt atctggtagt tcagcagtct tgatgggctt actagttggt ggtatgatgg
    11341 ctgtagatat gggtgggcct gttaataaag cagcttatgt ttttggtaca ggtactcttg
    11401 cagcaactgt tgcaaatggc ggctctgttg ttatggcagc tgtaatggct ggaggaatgg
    11461 ttccaccgtt agcagtattt gtggcaactt tattatttaa agataaattt aataatgaag
    11521 aacgtcaatc tggtctaaca aatatcgtta tggggttatc ttttatcact gaaggtgcta
    11581 ttccgtttgg tgctgctgac ccagctcgtg caatccctag ctttattgta ggttcagcct
    11641 taactggagc acttgttggt ttagctggta tcaagttgat ggctcctcat ggagggattt
    11701 ttgtcatcgc attaacaagt aatcctttac tttatattct atttattcta attggagcga
    11761 tagtctcagg tgtactcttt ggcttgttcc gtaaaacaaa atagttaatc tcaacttatc
    11821 caaatttaca ttgaataaaa ggtgaataaa ctatcgttat tgggtctatt cgccttttta
    11881 t
//
LOCUS       DATROY010000058        11810 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.059, whole genome shotgun sequence.
ACCESSION   DATROY010000058 DATROY010000000
VERSION     DATROY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            132..248
                     /locus_tag="VBD11_001191"
     CDS             132..248
                     /locus_tag="VBD11_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6954146.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(340..1224)
                     /locus_tag="VBD11_001192"
     CDS             complement(340..1224)
                     /locus_tag="VBD11_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6954147.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(1372..3036)
                     /locus_tag="VBD11_001193"
     CDS             complement(1372..3036)
                     /locus_tag="VBD11_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6954148.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(3036..3401)
                     /locus_tag="VBD11_001194"
     CDS             complement(3036..3401)
                     /locus_tag="VBD11_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6954149.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(3533..3721)
                     /locus_tag="VBD11_001195"
     CDS             complement(3533..3721)
                     /locus_tag="VBD11_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6954150.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            3960..4877
                     /locus_tag="VBD11_001196"
     CDS             3960..4877
                     /locus_tag="VBD11_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6954151.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(4962..5843)
                     /locus_tag="VBD11_001197"
     CDS             complement(4962..5843)
                     /locus_tag="VBD11_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6954152.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(5983..7371)
                     /gene="argH"
                     /locus_tag="VBD11_001198"
     CDS             complement(5983..7371)
                     /gene="argH"
                     /locus_tag="VBD11_001198"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6954153.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            complement(7390..8580)
                     /locus_tag="VBD11_001199"
     CDS             complement(7390..8580)
                     /locus_tag="VBD11_001199"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6954154.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            complement(8734..9813)
                     /locus_tag="VBD11_001200"
     CDS             complement(8734..9813)
                     /locus_tag="VBD11_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6954155.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVSSQLEIGTEFCVELPLS"
     gene            complement(9797..10468)
                     /locus_tag="VBD11_001201"
     CDS             complement(9797..10468)
                     /locus_tag="VBD11_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6954156.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(10499..11185)
                     /locus_tag="VBD11_001202"
     CDS             complement(10499..11185)
                     /locus_tag="VBD11_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954157.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(11185..>11810)
                     /locus_tag="VBD11_001203"
     CDS             complement(11185..>11810)
                     /locus_tag="VBD11_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954158.1"
                     /translation="SSSISLTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFL
                     PQALVMKNDKSISHLPTQLEAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGV
                     FFYIITLQKRHEFSVMKAIGTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPA
                     QMPFVINWQNIILVSFVFLVIAMISSALSIVKVAKIDPVEVINGGGE"
BASE COUNT         3614 a         2347 c         1861 g         3988 t
ORIGIN      
        1 attacttttg attgatattt aattactttg ctatttattt ttaaatttat caacaaagta
       61 ctttaccagt taattttttt gtggtatact ggtgtctgtt aacgttaata aattgtctat
      121 aggaggtaat catggctgaa aaaacagacc tatcgtcagc ctatcgcaga cttaaaagtc
      181 ctaacattaa aactagaaaa cgtgctctga aaatcattca tgagtacaaa cgttacggta
      241 aaaaataact tttttccgtc tctccaatta taataaataa gtcagacaca aaataaccag
      301 agcaaatgct ctggttattt tgcttctata aaaagttcat tatcttgttt taagagcaga
      361 taaaacttgt gtacgaatat cttcaacacc ttctgggtta tttggtaaga aaatcgtttg
      421 attaccatta atagcaaatg tatttaaagt atctaaatat tgatttgtta acaagataga
      481 catgatttgt tcttctgtca atgtcacgtt agcatctttc aattcttgaa tcgaatcagc
      541 taacccatct acaattgctt tacgttgttg cgcaataccc acaccatgta gacgatcttt
      601 ttctgcttct gcttctgcag cagttacaat tttgatctta tcagcattcg ctaattcttg
      661 agctgctaca cgtttacgtt gtgcagcatt gatttcgttc atggattgtt ttacttctgc
      721 atctggctca accttagtaa ttaaagtctt aacaatgatg tagccgtatg ttgacatttc
      781 ttctgccact tgatgttgaa cttccaaggc tatttcatct ttcttttcga atagctcatc
      841 taatgtaagt ttaggaactg atgaacgcaa agcatcttcg atataagatt taatctgagc
      901 ttctggcttt attaatttgt aataagcgtc tgtaacgtta ttttcattga cacgatattg
      961 tgtagcaata tttaaagtga caaaaacatt atcttttgtt tttgtctcaa ctataatttc
     1021 actctgtaaa agacgtaact gcactcgtgc tgcaatctta tcgataccca atggtacacg
     1081 aatatgaata ccgctagtag cagtcttttg atatttacca aagcgttcaa tgatggctac
     1141 cgtctgttgt ttaacaacgt ataaagaagt tatcaacaga acaatcacta aaaccaaaat
     1201 aaccgtaaga ataattaatg acatcataaa tctcctttgt atgaatgttt taaaacaatt
     1261 ctagcacaat ttgtcaaaat tttcaaaata ctgatgcttt atcatactaa aatagaaact
     1321 ctgataaaat aaaaaattag aagtatctct cctaattttt tattttatca attattcaac
     1381 gctcatgagg tatggatata caggttgatc tccttggtgg atctcaactt caacatcttc
     1441 ataagtctcc tcaagatatt ccgacaaggt ctcggctaat gcttgtttac catcttcacc
     1501 aacatagatt gtcacgattt cactatcttc atcaatcatt ttatcaaaag tgtctttaag
     1561 tgctttttcc atatctggtg tagatactag aattttacca tcaaccatcc caaggatgtc
     1621 gttttcatga atttcaaggc catctatcgt tgtatcacga acagctaaag tcacactacc
     1681 actaatgaca tctgagaggc tatttgtcat atcagctaca ttagtttcaa gagacttagc
     1741 aggatcaaaa gcaagtaagc tagtaaatcc ttgtggaact gtacgtgtct ctactactgc
     1801 tgctggaata tcaacgacat ctgcagctga ctgtgcagcc atgaagatgt tcttattgtt
     1861 tggtaaaata ataacatttc tagcgtttac tttttcaatc gcttttacaa tatcttctgt
     1921 agatgggttc atagtctggc cgccagagat gatataatca acaccttgtg atttaaaaat
     1981 ctcagctaga ccgtcaccag ctacaacagc aataatacca tattctttag tttctttgac
     2041 agtttcttca acttcaacct tttgcatttg ggcatcatgt tggttacgca tgttttcaac
     2101 tttaaccttc acaagcgatc catatttcaa accttcttgc ataacaagac ctggatcttc
     2161 tgtatgaaca tgaaccttga caatttcatc atcattaaca actagtaaag aatcccctag
     2221 attgctaaga taaccttgaa attcttcgta gttaaattcc ttaacgtaag ttggtccttg
     2281 ctttaaacca accatgactt ctgtacaata gccatattta atatcttctg tggctacgtg
     2341 gcctactaca gctttatggt gctctgcatt aaccatttca gtcattgttg cgggagttgc
     2401 tttaaaatct tctgatgcga tatattcccc agtcagtgct gagaggaaac cttcgtaaat
     2461 aaagacgagt ccttgaccac cagaatcaac aacaccaacc tctttaagaa caggtagcat
     2521 atccggtgtt ttagcaagag cacgcttagc acctttgaga gttgcgcgca taacttcaac
     2581 tgcatcatca gtttcttcag ctttttttag agcagcggta gctgcaccgc gtgatactgt
     2641 caagatagtt ccttcaactg gtttcataac ggctttataa gctacttcaa caccattctg
     2701 gaaagcgtgt gccaaatctt gacccgttaa ttcttcttta tccttgatac tttgaccaaa
     2761 accacggaat aattgtgatg taattacacc tgaatttcca cgtgctccca tcaatacacc
     2821 tttagaaaga atttgaccaa cttctccaac agtcgtcgct ggtttgtccg aaacttcctt
     2881 ggctccgttt tccatagtca tccccatatt cgtaccagta tcaccatctg gaactgggaa
     2941 tacatttaat gagttgacat actctgcctg tttactcaga cgtgtactgg cagcttgtac
     3001 catttcttgg aataaacttg tcgtaatctt tgacactaat cttctcctac aaccttaata
     3061 ctttgcacat aaacattcac catatcagca gataaaccaa gctgattttc gagattgaat
     3121 ttcactcgct cttgaatatt ttttgaaact tcactaattt tgacgccgta actcatgaca
     3181 gtataaacat ctactgcgat gcccgcttca ctagacctca caacaacacc ttttgcgtag
     3241 ttttccttac gcaaaagtgc ttgaaagtta tctttgatag cacttttact tgccatacca
     3301 acaacgccaa aaatttccgt tgctgaacct ccaacgacag ttgcaataac gtcatcagag
     3361 agttcaattt gaccatctct tgtattgatt tttacagtca tatctactac ctcaaaaagt
     3421 ttttatcact ctattctatc atatctcact attattgtaa aaaaatagag gtatctaact
     3481 atcaaatcag ccttagaaac actaaaaaaa gccctaaggc tttctcagtt tattaaacgc
     3541 gttctacttt accagattta agcgcacgag ctgaaaccca aacttttttt ggtttaccgt
     3601 cgataagaac agtaactttt tgaaggtttg gtttaacagt acgttttgtt tggttcatcg
     3661 cgtgtgaacg gttgttacca gaaactgttt tacgtcctgt gaaataacat actttagcca
     3721 ttttacgagt tcctcctata ttgatcaata tttacggatg tgctagcacc acatactaga
     3781 ctattctatc acaacttact tcttctagca agttcttttc aaaattactt tttcatttgt
     3841 tttatctaaa tcgcttacat attttttata ataagacaca cttccttcta attcattttt
     3901 tagtttacat tttctttcaa aatattataa tagacaacaa taatcaaagg aggtttcata
     3961 tgacacgtaa aattggaatt ataggcatgg ggcacgttgg atcaactgtt gcacacgctc
     4021 ttattgcaca aggtattggt gatgattatg tctttattga tactaatgtt gataaggtta
     4081 atgctgatat gattgatttt caagatgctc tggcaaatgc tacttatcac gctaatatag
     4141 tattaaacga ctatgacgcg ttgtcagatg ctgatgttgt gatttcagcc ttaggtgata
     4201 tttcactaca gaataacaaa gaagctaatc gctttgctga actaccattt accagtaaag
     4261 aagtctacga cgttagccaa aagttaaaat catctggttt tagagggatc cttattgtcg
     4321 ttactaatcc tgttgatgcc atcacccagc tttatcaaga atatactcaa tttcctaaat
     4381 cacgcgttat cggaacagga accttattgg atactgctcg gatgaaaaga gtgcttggtc
     4441 aacgcctaca agttgatcca aggagtatcg gtggttataa tctaggagag catggtaact
     4501 cccaatttgt cgcttggagt caggtaactg taaaaggaca accagctata tcactattaa
     4561 aagtagaaga attagaacag ataaaagaag cttctttaaa aggcgggcac actgtctttt
     4621 ttggtaaaaa atatactagt tatggtatcg ctaccgcagc tgtccgcatc gttttagctg
     4681 tgttaaatga ttcccatgag ttactacctg tatctacctt ctatgagtcc gaaggggttt
     4741 accttggata tccagctatt attggtagag taggaattgt agaaagaatt tctctttcac
     4801 taagcccaaa agaagagcaa caactcacag attcagcggc atttattcga aattatctta
     4861 agcatatcaa tctttaaaga gtatctattt aataaaaagt catataatcc aaaaaaagct
     4921 caccataagt gagcttttaa ctattatctg ttttatgtta attatgcttt gtttgctgaa
     4981 ccaaacacgt caatacgttc ttcaactgct tctgtaattg catcgaaacc aggtttcaag
     5041 aatttacgtg ggtcaaagag ttttttcttc atgtattcag cttcgttagc gttgaattca
     5101 acagcgtagt cacgagttgc ttgacagaaa gctaattgac attctgtgtt aacgttaact
     5161 ttagcaacac caagtttgat agcttcttgg atttgatcat caggaatacc tgaaccaccg
     5221 tgtaacacga ttgggaaacc aggaacagct tcagtcaatt tcttcaagtg atcaaggtca
     5281 agtccttccc agtttgctgg gtatggtcca tgaatgttac cgatacctgc agccaagaag
     5341 tcaataccag tttcaaccat agctttagcg tcttcgattg gtgctaattc acctttaccg
     5401 acaataccat cttcttcacc accgatagta ccaacttcag cttctactga aataccttta
     5461 gcatgtgctt tagcaacaac ttcgcgtgct ttttcaaggt tttcttcaac tggaaggtgt
     5521 gaaccatcaa acataattga agtgtaacct acttcaatac actcaagggc atcatcgtaa
     5581 tgaccgtggt caaggtggat agcaactggt acagtaatac ccattgattc aaccaaagtt
     5641 tcaataagtt gtttacaaag tttgtaacca cccatgtatt tagccgcacc cattgaagtt
     5701 tggataagta ctggagcttt tttagcttct gctgcgcgca agatagcttg agtccactca
     5761 aggttgtttg tgttaaatcc acctactgca tagccgttat cacgagctgc ttggacgaat
     5821 ttttctgctg aaacgattgc cattattcag gcctcctatt tattttttgg gcgttgccca
     5881 cttacatgtt aattctagca caattctata aaaaatgcta gttttttact gaaaatacgt
     5941 gagaaactta acagagttaa acgaggtttt ttcagaaagc agtcattccg ctttcaattc
     6001 tttacgtgcc aacaggatct gttctttaac ctggtcaaac cctgttcctc ctaatgaatt
     6061 tcttcgttta actgctgtct ttgcagttaa tatctcatag atatcgtttt ctatcaactc
     6121 tgaaatttct tgataatatt ttaaaggtat atcctgtaaa taagaaccat ttttactgca
     6181 ttctaaaacc aatttaccta caatttcatg tgcttttctg aaaggtaccc ccttacttgc
     6241 taaataatct gctaattctg ttgcatttga aaaatcagtc tccgttgagg tcatcattat
     6301 gtgctcattc acagtcattt tctcaagcat attagccatg atttcaatag ctatgctgac
     6361 tgtttctacg ctatcaaaca tcccttcttt atcttcttgt aaatctttgt tataagctaa
     6421 cggcaatgat ttcataacag ttagtaaact aaataaattt ccataaacac ggcctgtctt
     6481 ccctcgaatt aattctgcca tatcaggatt tttcttttga ggcataatag atgatcctgt
     6541 agagaaagta tctgataatg tgatgaattg atattcataa ctgcaccaat taatgatttc
     6601 ttcacaaaaa cgtgataaat gcatcattaa aatgctagca ttggataaaa attctatgat
     6661 aaagtcacga tctgagacag catcaagcga attactatat ggtttttcaa aatctaataa
     6721 acgagttgtc atatggcggt caataggaaa tgttgtccct gctagagctg ctgctcctaa
     6781 tggtgataga ttggtgtgtt tcatattaaa ctctaatcgc tctgtatcac gcgtaaacat
     6841 attatagtaa gccattagat gatgtccaaa tgaaatcggc tgagcatgtt gtaaatgtgt
     6901 atatcctggc ataacagtat agatatgatt ttctgctaaa ttaactaagg ttgttcgtaa
     6961 atgaagcaat ttcttcatca tttcttgcaa tttatctttc aaataaaggt gcatatctgt
     7021 agccacttga tcatttcgtg aacgtgcagt atgaagctta cctgctactt ctccaatttc
     7081 agcggttaat aagctctcta tgttcatatg aatgtcttca tttgatatgc taaaagtcaa
     7141 ttgacctgct ctgtatttat cttgcagtat ttttaaacca tcttttatca agcctgcttc
     7201 ttcttgacta ataatatccg ttttgcctaa cattgtgaca tgggccatag aagccttcat
     7261 atcataggga gctaactttt ggtcgaaact gattgatgca ccgaactctt caacccattt
     7321 ttcaagacta ctttcaaaac gaccacccca taacttatga ttttttgtca tcctcttatc
     7381 ttcctttatt tatcctttgt ttacttgtgc attaacttgg gttggaagtc cccagagttt
     7441 gataaaacca actgctgcat cttgatcaaa gttatcagca gctgtataag ttgctaagtt
     7501 ctcatcatat aatgaattag acgagtggcg agcaacgact ttggcagaac ctttatataa
     7561 cttaactttt gtagtaccat taaccacttt ttgtgtttct ttgacatagg caatgatagc
     7621 ttttgtagct ggactaaacc ataaagcatt ataaataaga tttgatagtt cattttcaag
     7681 gataggttta aaatgagaca cttcacggac taatgttaaa tcttcaattt ctttatgagc
     7741 agctaacagt accattgccg caggacattc ataaatttca cgtgatttga taccgactaa
     7801 acgattttca acatgatcaa tgcgtccaat accatgtttt cctgcaattt catttagcga
     7861 taaaatcaaa tctgctagtg tcatttcttg attgtttata gctataggct tcccattttg
     7921 gaacgtaata tcaatatatt ctgcacaatc aggagcttct tctggtgatt ttgtaatacc
     7981 aaaagcttct tctggcgctt gattccaagg attctcaagc acaccacatt cattggcacg
     8041 tccccataaa ttttggtcta tagaataagg gttatctaaa tctgctggaa taggtacgcc
     8101 gttagctttt gcaaaagtaa tttcctcttc acgatgccac tgccactcac gaacaggggc
     8161 aattactttt aattcaggat ctagtgctgc aattgcaact tcaaaacgca cttggtcatt
     8221 tcctttacct gtacatccat gtgcaattgt ggtcgcacct gtttgatgtg ccatttcaac
     8281 taatttttgg gcaataattg gacgactaag cgctgaaaca agcgggtatt tttgttcata
     8341 catcgcatgt gcctgtaaag ctggtaaaac aaaatgctca gcgaactcat ctttaacatc
     8401 taaaatataa gattctatgg cacctattgt taaagcttta tcgtgaataa aatcaagatc
     8461 tttaccttcg ccaacatcca tacagacagc aataacgtca taatcttttt ttaaccaagc
     8521 tatagcaaca gaggtatcta aaccaccaga ataagctaaa attaattttt cttttcccat
     8581 aaaaaatccc cttccaaatt cctatatttt gtaatattat acacttgcta tatttattat
     8641 caaatatttt tatagttttt gtctattttt agtatattta tttctaaaca aataaaacaa
     8701 taactgccaa ttggcagtta ttgttttaaa taattatgat aaaggtagtt ctacacaaaa
     8761 ttctgttcca atttccaact gactggagac actaatatgt cccttacaaa gaacaattat
     8821 acgtttaact attgctagtc ctaatccatt tccttgttga ttatgcgact gatccgcttg
     8881 gtaaaattta tcaaatatat gctgaacatc atattgagaa attcccctac ctttatcaga
     8941 aataataact ctcagataat gattattagt ttcttccatt cgtacactta agtctacaat
     9001 atcctcggaa tattttattg cattatctaa aaggtttatc caaacttgca tcagcaagtc
     9061 agagtttgag taaacagtat atggttttga atccagttga aaatttatcc ttttagcttg
     9121 ccaacgttct gttaaactaa tcattgcttg acgaatttgt tcatctacac gaacagagga
     9181 tagctcccca atagtctcct gattatccac gcgcgacatt tgcaacatcg tatcacataa
     9241 tcttgttaac cttaggctct ctgttttaac aagacctaat aactcacttt gtgtttcttc
     9301 atctaattct ggatcttgta atagttctac aatattagct actgcagcaa tcggagtctt
     9361 tagctcatga gaaacattag tgataaagtc ttttcgcata tgatccatat gagataaaga
     9421 aacaatcatc tgattaatac tttcagatag ctcatccaat tcattataat actcatagga
     9481 tttacgacga ttactgtttc gataaatatg cccttcaaag tcaccgcctg caacattgga
     9541 cacaatatgg ctaatatcta aaattggttt tgttaaatga taagaaccgt accacatact
     9601 aatactagct atcacaaggc ttaaaaaaac aacagcgaga gcgctaactt gtaatacatg
     9661 agtagttaag actccttgaa aaaaagtaaa aactattgct gcaactagta aaagagaagc
     9721 taggactgag aaaagcatcg ttaacacaaa cattaatgtg aaataaaatc ttaacggaaa
     9781 ccgaaatcgc cttagcttag tcattttttg cacgataccc tacccctctc accgtttcaa
     9841 tcttaaaatc tggtaagtgt tctacttttc gtctaatttt attgatgcaa gcatctacca
     9901 cccgctcatc taaatcagta tccattcccc aaatactatc caatagttct aaacgtgtga
     9961 aaatcctatt aggatagcta agtaagtgga aaagaaccct aaattctttc tgaggaaata
    10021 caatttcttg ctcaaaatat ttcaagctta attcatcttc attaagacaa gtattcccaa
    10081 taattagctg atgttgagct acaatattag ctctcctcaa gagtgcctta atacgtaaag
    10141 ctaattcttg taactgcaca ggcttaacca tataatcatc tgcaccaagg ccaaaccctt
    10201 ttgtcatatc ttccatttgt gattttgctg tcataacaat gattgggaca ttgtaagcag
    10261 ctgacctgag ttcttgaatt aattgataac catccatcat gggcatcatg atatcagtta
    10321 taatcaattg tacttcttct gtatctagac aatttagagc ttcttgtcca tttgaagcag
    10381 tgataaattg gtagccctct tgtgttaatt ttgtggtaat gattttttgt tgaacaatgt
    10441 tatcctctac aactaatatt ttaatcatta aatcacctct agcttattgt aacacaagtt
    10501 agaagttttc tttaactaat ttcccgtcct ttaaacgata aacggtatct actaaatcta
    10561 gaacgcgctc atcgtgtgtc accataatag ctgcagtatt atgtgattta acttcctgac
    10621 gaataagttc tgtaacttga cgcccacgtt cttcatcaag gctagctgtt ggctcatctg
    10681 ccaagataac cttaggatga ttaataaatg ctctagcaat tgctgcgcgt tgcttttgtc
    10741 ctcccgataa ttggttaggg tatttatgtg cacactgctc tattcctaaa tctgataata
    10801 atttattaat ttcttccaag cttgttttct tatcatagtg ttttgcaaat cgagcctctt
    10861 gttgcaactg ttcttggatt gttaaataag gaagcagttg atgactttga aatataaaac
    10921 ctagtgcttc acggcgcaat tgtgttcttt gtctttgaga caaatccgta acgttctcat
    10981 gattgacata caaactacct tgatctgcac caagaagtaa ccctgcaatg gataaaagtg
    11041 tgcttttacc tgaacctgag ggtcccagta tagctacaaa ctcacctgca gaaactgata
    11101 aatctaaatt atctaatatt gataaaagct catctccatc tggatagtgt tttgagatgt
    11161 gttttaattc taagattgct gacattattc tcctccccca ttaataactt cgactgggtc
    11221 gattttagca acttttacaa ttgacaaagc actactaatc attgcaatga ctaaaaatac
    11281 aaacgatact agaataatat tttgccaatt aataacaaat ggcatttgag ctggtaatac
    11341 ataagagaga gcaatcgcca atcgatctcc aacaatgata ccaaacagtg ccaatatgat
    11401 gacttgactc aattgaaata gggcaatctc tgacattttt gtcccaatag ctttcatgac
    11461 gctaaattca tgacgttttt gaagtgtgat gatgtagaaa aagacaccta aaataccagc
    11521 tgaagctacg acaaggaccc ataaaatcat attcaaagtg gattgttcag cactataacc
    11581 aggaatatgt tgaatgacat cttttttact aacagcttct aattgagttg gtaaatgaga
    11641 tatactttta tcatttttca taactaatgc ctgaggtaag aactggtatt gaggattaat
    11701 ctttttatta atttcggtat aaatgtcttt atcaataaat gccacaggtc cgtgaccata
    11761 catactatta tggacaaatc caacgactgt tagtgatata gatgatgact
//
LOCUS       DATROY010000059        11774 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.060, whole genome shotgun sequence.
ACCESSION   DATROY010000059 DATROY010000000
VERSION     DATROY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11774
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..765
                     /locus_tag="VBD11_001204"
     CDS             <1..765
                     /locus_tag="VBD11_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6954159.1"
                     /translation="SNYQFSYKETLRELKKQHKASDLQNQMRRRGSNALSRITRLTRL
                     ISKDNINSLRRKGISSPYRKMSSEESKELSRLKRFSYLSPLISFVVIIYTLFLNYFTY
                     FCIYLLLFGVILLLNKIIFMMTRKISNGYIVTEDGASRVYQWTSFRNMLRDIKSFDRS
                     ELESIVLWNRILVYATLFGYADRVEKVLRVNQIDIPERFANIDSHRFAISVNQSSNHF
                     STITEDVSHASNFSVNSGGSSGGFSGGGGGGGGGAF"
     gene            811..1650
                     /locus_tag="VBD11_001205"
     CDS             811..1650
                     /locus_tag="VBD11_001205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6954160.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            1770..2525
                     /gene="mecA"
                     /locus_tag="VBD11_001206"
     CDS             1770..2525
                     /gene="mecA"
                     /locus_tag="VBD11_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6954161.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            2527..3687
                     /locus_tag="VBD11_001207"
     CDS             2527..3687
                     /locus_tag="VBD11_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6954162.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            3852..4622
                     /gene="sufC"
                     /locus_tag="VBD11_001208"
     CDS             3852..4622
                     /gene="sufC"
                     /locus_tag="VBD11_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6954163.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            4659..5921
                     /gene="sufD"
                     /locus_tag="VBD11_001209"
     CDS             4659..5921
                     /gene="sufD"
                     /locus_tag="VBD11_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6954164.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            5923..7155
                     /locus_tag="VBD11_001210"
     CDS             5923..7155
                     /locus_tag="VBD11_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6954165.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            7142..7585
                     /locus_tag="VBD11_001211"
     CDS             7142..7585
                     /locus_tag="VBD11_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6954166.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            7685..9103
                     /gene="sufB"
                     /locus_tag="VBD11_001212"
     CDS             7685..9103
                     /gene="sufB"
                     /locus_tag="VBD11_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6954167.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(9174..10361)
                     /locus_tag="VBD11_001213"
     CDS             complement(9174..10361)
                     /locus_tag="VBD11_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6954168.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKLGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(10514..11749)
                     /locus_tag="VBD11_001214"
     CDS             complement(10514..11749)
                     /locus_tag="VBD11_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6954169.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
BASE COUNT         3700 a         1869 c         2356 g         3849 t
ORIGIN      
        1 tctaattacc aatttagtta taaagaaaca ctacgtgaac tgaaaaagca gcacaaggct
       61 tcagatctgc aaaatcaaat gagacgccga ggaagtaatg ccttatcaag aattacgcgt
      121 ctcacaaggt tgatttctaa agacaatata aactctctta gaagaaaggg aatttcatcc
      181 ccttatcgta aaatgtcttc agaagagtct aaagaattat ctaggttaaa aagattcagt
      241 tacctatcac ctcttatttc ttttgttgtt ataatttata cgcttttttt aaattatttt
      301 acctatttct gtatctatct cttattgttt ggtgttatcc tgttgttgaa taaaatcatt
      361 tttatgatga caagaaaaat aagtaacggt tatattgtaa ctgaagatgg agcaagtcgt
      421 gtctaccaat ggactagttt taggaacatg ctaagggata tcaaatcgtt tgatcgttca
      481 gagttagaaa gtatcgtatt atggaatcga atattggttt acgctacttt attcggctac
      541 gctgaccgtg ttgagaaagt actcagagtg aaccaaatag atattccaga aagatttgca
      601 aacattgata gtcatcgatt tgcgatttca gtcaatcaat ctagtaatca tttttcaacg
      661 ataactgaag atgttagtca cgcttctaat tttagtgtta attcaggcgg ttcttcaggt
      721 ggtttctcag gcggcggagg cggcggaggt ggcggtgcct tctaattttc caaaagtatg
      781 gtataataaa gaaaaattta tggagttatt atgttaataa ttgaattact caaagcccta
      841 tttttaggag tcgttgaggg agtgacagaa tggttacctg tttctagtac tggccattta
      901 atcttggttc aagagtttat gaagttgaac caaagcaaat cctttgttga aatgtttaat
      961 attgttatac aacttggggc aattatggca gttattgtca tttattttaa acgccttaac
     1021 ccatttcagc caggtaaatc tgctagagaa attcgtttaa cttggcaatt atggttaaaa
     1081 gtagtgatag cttgtattcc atctatttta attgctttac catttgataa ttggtttgaa
     1141 gctcatttta atttcatgat tccgattgca atagccctaa tcttttatgg ttttgtcttc
     1201 atatgggttg aaaaacgtaa tgcacacctc aaaccacagg taaccgaatt ggcaagtatg
     1261 tcttacaaga cagctttctt gattggatgt ttccaggttc tcagtattgt tccgggaacc
     1321 agtcgttctg gagctactat tttaggagca attattattg gaactagtcg ttcggtcgct
     1381 gctgacttta ctttcttcct tgccatccca actatgtttg gttatagtgg acttaaggcg
     1441 gttaaatatt ttttagatgg taacgtcttg agtttagacc aatctttaat acttttagta
     1501 gcaagtctga cagctttcgt agttagttta tatgttattc gtttcttgac agactatgtc
     1561 aaacgacacg atttcaccat ctttggtaag tatcgtatag tcttaggaag tttactcatc
     1621 ctctactggt tagttgttca tttattctaa aatataacat aaaaagctga gtattcctca
     1681 gctttttagc gtgttaggct gtgaaaaatt gctaaaaaat agctttttcg gtataataag
     1741 aaaactgaat actcgtatga ggtgatgata tggaaatgaa acaaatcagt gaaacaacac
     1801 tgaaaattac aattagtatg gaagatttag aagatcgtgg tatggagctg aaagatttcc
     1861 taatccctca ggagaagact gaggaatttt tctattctgt catggatgaa ttagacttgc
     1921 cagaaaactt taaaaatagt ggtatgttaa gttttcgagt aacacctaaa aaagatcgca
     1981 ttgatgtttt tgttacaaag tctgaattaa gtaaagattt aaatttagaa gaattagcag
     2041 atttgggtga catttcaaaa atgtctccag aagacttttt taaaaccttg gaacaatcga
     2101 tgttggaaaa aggggatacg gatgcccatg ccaaattagc agaaattgaa aatatgatgg
     2161 ataaagcaac tcaagaagta gttgaggaaa atgtttctga agaacaacct gaaaaggaag
     2221 tagaaacgat tggatatgtt cactatgtct ttgattttga taatattgaa gctgtagttc
     2281 gattttcaca aacgattgat tttccaatag aagcttctga actctataaa aatggtaaag
     2341 gctatcatat gacaattttg ctagacttgg aaaaccaacc aagctacttt gctaatttga
     2401 tgtatgctcg aatgcttgaa catgctaatg tgggcacaaa aacacgagct tacttgaaag
     2461 agcatagtat tcaattgatt catgatgatg ctatttcaaa attacaaatg attgagatgg
     2521 gataaaatga taccttttac aattgagtat atttttgtat tgattggagc atttttatta
     2581 tcaatcattc taactcctat tattcgagtt atatctttga aggtaggagc ggttgataaa
     2641 ccgaacgctc gaagaatcaa caaagtacca atgccaagta gtggtggttt agcaatattt
     2701 ctctcctttg ttgtaacaac attatttttc atgccaatgg cggccagtag acactttata
     2761 gaagtctctt attttcacta cattttgcct gtaattattg gcggtctcgt agttacaaca
     2821 acaggattta tagatgacat ctttgaatta agacctcgtt ataaaatgtt gggaattatt
     2881 attgcagcta ttatcatatg gaaatttaca cattttagat ttgatagttt taagattcca
     2941 ataggtggtc cactcttgga gtttggtcct attttaactt tctttttgac agttttatgg
     3001 attatttcaa tcactaatgc tatcaatctg attgatggtc tagatggtct agttagtggt
     3061 gtttcaatca tcagtctagc aacgatggca gtggtttctt atttcttttt acctaaaata
     3121 gatttttttc tcacgctaac tattgttatt ttaatcgcct caatagttgg ttttttccct
     3181 tataattatc atccagccat tatctaccta ggtgatgctg gtgccttgtt tataggattt
     3241 atgattggtg tattatcgct tcaaggttta aaaaactcca cagctgttgc agtgataaca
     3301 cctgtcatta ttttaggagt tcctattttg gatacagctg ttgctattgt gaggcgtaaa
     3361 ttatcaggga aaaagatttc tgaagctgat aagatgcacc tgcatcaccg attgctatct
     3421 atgggattta cacatagagg agcagtttta gttgtttatg gtattgcaat tatcttttcg
     3481 cttatcgctt tgcttttaaa tgtttctagt cgtattgggg gaatttttct tctattggct
     3541 ctactattag taatggaaat ttttatagaa ggtttaaata tttggggtga gaatcggaca
     3601 ccgctattta atctcttaaa gttcattggc aacagtgatt acagacaaag tgttattgct
     3661 aaatatagcg ataagcatca aaaataaaag cctgaaaagg cttttttagt tttaatcatt
     3721 tagatagcag tcacacctca tttttgctat aattatttag aatataatat cgggtgagtg
     3781 ccctataata tcttctttaa accatagaga aatatcacat attctctgtt aaccaagaaa
     3841 aggagtgtac aatgtctgta cttgaaatca aaaatcttca tgtttctatt gaagataaag
     3901 aaattttaaa aggtcttaat ttaacgctga aaacaggaga aattgctgct atcatgggtc
     3961 caaatggtac tgggaagtcg acactttcag cagctattat gggaaatcct aattatgaag
     4021 ttacagcagg tgaaattctt tttgatggtg aagatatttt agagctagaa gtggatgaac
     4081 gtgctcgttt gggtctattt cttgcgatgc aatacccttc agaggttcca gggattacta
     4141 atgctgaatt tatccgagca gctatgaatg cgggaaaggc tgatgatgat aaaatttcca
     4201 tccgccaatt tattactaag ttagatgaaa agatggaact acttggcatg aaagaagaaa
     4261 tggcggaacg ttatcttaac gaaggttttt caggtggtga gaagaagcgc aatgaaattt
     4321 tacaactcct aatgttagag cctaagtttg ccctcttgga tgagattgac tctggcctgg
     4381 atattgatgc tcttaaagtg gtatcaaagg gagtcaatga aatgcgtggt gaaggttttg
     4441 gtgctatgat tattacacac tatcaacgcc ttttgaatta cattacacca gataaagtac
     4501 acgttatgat ggacgggaag gtagtattgt caggaggacc agaattagcg gttcgccttg
     4561 agaaagaagg ttatgctcaa atcgctgaag agcttggttt agagtataaa gaagaagtct
     4621 aatttgtcac ttcaactaga agagaaaagg agttacaaat gtctaaagaa gctatattaa
     4681 attttttaca agccaaaggt gagcctactt ggttacaaga attacgacta aaggcttttg
     4741 aaaaaataga agaactagaa ttaccagtaa ttgaacgtgt aaaatttcat agatggaatt
     4801 taggtgatgg aactatttta gagaatgact atacagctaa cattccagat ttcacagagt
     4861 taggaaacaa tcccaagttg gttcaaattg ggacccaaac tgtccttgaa caagttccta
     4921 tggaattaat agagaaaggc gttgtcttta cagatttcta ttcggctttg gaagaaattc
     4981 cagaagtcat agagcgctac tttgggaaag cacgtccttt tgaagaagat agacttgctg
     5041 cctatcacac agcttacttc aacagtggtg cagtgcttta tattccagat aatgttgaaa
     5101 ttactcaacc aatcgaaggt cttttttatc aagatagtca atctaaagta ccatttaata
     5161 agcatatcct tcttattgtt ggaaaaaatg caaaagttag ttacttagag cgatttgagt
     5221 caattggtga tgggacagaa cgaacaagtg ctaatatttc tgtagaagta attgcccaag
     5281 caggtagcca aattaaattt gcctcaattg atcgcctcgg agaaaatgtg acaacgttta
     5341 taagtcgtcg tggtcgtcac agtagcgatg caacaataga ttgggccctt ggggtgatga
     5401 atgagggcaa tgttgttgct gactttgatt ctgatttaat tggtgatgga agccacgcta
     5461 atttaaaagt agttgctgct tcgtcagggc gtcaagttca aggaattgat acgcgtgtta
     5521 caaactacgg ctgtaattca gtaggacata ttttacagca tggggttatt ttagagcgtg
     5581 ggactcttac ttttaacggt atcggacata ttataaaagg tgcaaaggga gcagacgccc
     5641 aacaagaaag ccgagttctc atgttatcag acaaagcacg ttcagatgct aatcctatcc
     5701 ttttgattga tgaaaatgat gttacagctg gtcacgcagc ctcaattggt caggttgacc
     5761 cagaggattt atattatttg atgagccgtg gcttgaacca aaaaacagca gagcaattag
     5821 ttattcgtgg atttttagga acagtcattg ctgaaatacc agtaaaagaa gttcgcgatg
     5881 aaatgattgc cgttattgat acaaaactag aaaaacgtta atatgacatt actagatagt
     5941 tataaactaa agcaagattt cccaatttta aatcaattag ttaatgatga gccgcttatc
     6001 tatttagata atgcagcaac gacacaaaaa ccgaatcaag ttttagaaac tttgcgagat
     6061 tactaccaaa atgacaatgc aaatgtccat cgtggtgttc atactttagc agaacgagcg
     6121 actgctcagt acgagactgc tcgtgaaaaa gcaaggcagt ttttaaatgc taaatcaagt
     6181 aaagaaattt tattcacacg tggcaccaca acaggattaa attgggtagc taagtttgcc
     6241 gaaagtattt tagaacgagg tgatgaggta cttatttcca ttatggagca tcattctaat
     6301 attatcccat ggcaacaagc ttgcgagaga actggtgcta aattggttta tgcctatcta
     6361 aaagatggtt ctttagattt agaagatttc tataataaat tatcctctaa aactaaattt
     6421 gttagtttgg cacatatctc aaatgtactt ggctgtatta caccagtaaa agcaatagca
     6481 gaacgtgtgc atcaagttgg ggcatacatg gttgttgatg gagctcaatc agcgccacat
     6541 atggcgattg atgttcaaga ccttgattgt gatttcttcg ccttatctgg ccataagatg
     6601 cttggtccta ctggtattgg tgttctttat gggaaagaaa gcattttaga taagatgcca
     6661 cctgtcgaat ttggtggtga aatgattgat tttgtctatg agcatagtgc aacatggaag
     6721 gaattgccct ggaagtttga agctggaacg cctaatattg ctggagcgat cgcttttgga
     6781 gaagctttag attatttaac ggatgttggt atggatgaga ttcatcaata tgaacaaagt
     6841 ttagtttctt atgtgttgcc taaattacag gctatcgatg gtttaactat ttacggacct
     6901 agtgatgctg aatctcatgt aggcgttatt gccttcaatt tagaaggttt acacccacat
     6961 gatgtggcaa ctgcaatgga ttacgaagga gtggctgttc gtgcaggtca tcattgtgca
     7021 caacccctta ttaatcatct tggtatccat tcggcggttc gagcaagttt ctatttctat
     7081 aataccaaag aggattgcga caaattggta gacgctatcc aaaaaactaa ggagtttttc
     7141 aatggcactc tctaaattag ataacctcta catggcggtg gtagctgatc attcaaaaca
     7201 ccctcatcat catggatttt tagaaggcgt tgaacaagtg cagttaaaca atcctacatg
     7261 tggagatgtt atttccttat ctgttaaatt tgatgggaat attatttcag atattgcatt
     7321 tgctggtaat ggttgtacaa tttctacggc ttcatcaagc atgatgacag atgcagttat
     7381 tgggaaaaca aaagaagaag cattgcagct tgctgatgta ttctccaaaa tggttcaagg
     7441 caatcaaaat ccaaaacaag agaaacttgg ggatgcagag tttctagctg gggtatcaaa
     7501 atttccacaa cgtatcaaat gtgctacgct ttcgtggaat gctttaagaa aggctattga
     7561 gagagataat caggcagaac tctgacaacc atagttgaag gaattacatt taaacaactt
     7621 ttactgacat aaggtagtct agtagaagaa tgtcttaaat ttaaatatta aagaaaggat
     7681 aagtatgtcc gaaataaatg aaaaagtaga accacagcca atagatcttg gggaatataa
     7741 atttggattt catgacgatg taaagccaat ttattctacg ggaaaaggtc taaatgaggc
     7801 tgttattcgt gagttatctg cagctaaggg tgaacctgag tggatgttgg actttcgtct
     7861 aaaatccttg gaaacgttta ataaaatgcc gatgcagacc tggggagcag atttatcaga
     7921 tattgatttt gatgatatta tttattatca aaaagcatct gataaacctg cgcgtgattg
     7981 ggatgatgtt ccagaaaaaa tcaaagaaac ttttgaaaga attgggattc cagaagctga
     8041 aagagcctat cttgcaggag catcagcaca atatgaatca gaagtagttt atcacaatat
     8101 gaaagaagaa tatgataagc tgggtattgt ttttacggat actgactctg cacttaaaga
     8161 gtacccagag ctattcaaaa aatattttgc taaacttgtc cctccaacag ataataaatt
     8221 agctgctctg aactctgctg tatggtcagg tggaacattt atttatgttc ctaaaggtgt
     8281 taaggtggat attccacttc aaacttactt ccgtattaat aatgaaaata ctggacaatt
     8341 tgaacgtact ctcattattg ttgatgaggg agcaagtgtt cactatgttg aaggttgtac
     8401 cgccccaact tattcttcaa atagtttaca tgcagctata gttgaaattt ttgcacttga
     8461 tggagcttat atgcgctata cgactattca aaattggtcc gataatgtct ataatttagt
     8521 gacaaaacgt gctaccgcta aaaaagatgc aacagttgag tggatagatg gaaatctagg
     8581 agctaaaaca acaatgaaat acccatcggt ttaccttgat ggtgaaggag cacgtggcac
     8641 gatgttgtct attgcttttg caaacaaagg acaacaccaa gatacgggtg caaagatgat
     8701 tcataatgcc ccccatacta gttcatccat tgtctctaaa tcaattgcta agggtggggg
     8761 aaaagttgat tatcgaggtc aagtgacatt taataaagat tccaaaaaat cagtgtcaca
     8821 tatagaatgt gacaccatat tgatggatga tatttcaaaa tcagatacca taccgtttaa
     8881 tgagattcat aattcacagg ttgctttaga gcatgaagca aaggtgtcta agatttctga
     8941 agagcaactg tactacttga tgagtcgagg tttatctgaa gctgaagcaa cagaaatgat
     9001 tgttatgggg tttgttgagc cctttacgaa agaattacca atggaatatg cggtagagtt
     9061 aaatcgttta atttcctatg aaatggaagg ttcagttggt taattgaaga tttcaagaga
     9121 taataaaaag cccctatgtt attgatgaag gggcttttat attagcacag ttattatcta
     9181 gagcgcttta taagtaatac tatcccagct aaaagacaaa ggctagcgat ggtgcccaga
     9241 acaattaaaa tagatacaaa atgtgattgg ataaattgat ctgtttttgt ccaccactgt
     9301 tcttttgttt gaggctgttc ttgaatagat ttagttttgg ttttagtaac ttctttttta
     9361 ggtttaacgg cttttaatac aggagtttta gaagaaatat ttttagcgtc tttaaaacct
     9421 ttttctacaa gagcgttgac aaacggatga cgatagtact ctccgtcttg gtctgaccaa
     9481 tcgccaactc ctaaaaccac agttatcaag cgagtattct ggcgtttagc tgtaactaag
     9541 gcattaaaag cagcgctagg gctagaacca gtttttaagc catctactcc ttctaatccc
     9601 aatttagcgc cgggggtaga gtagttataa gttgtaaatg tttcttcata aggagttccg
     9661 accatggcct tgacttcagg atattttgta tagttcagta tatcagggta ttttttaagg
     9721 aaatgatagg ttaaaattga tagatcacgt gcagtcgtaa cgttagtagc attgttatcg
     9781 tattcttttg gggagtaaag tccattaaaa gcactcgcta ccgccccact ggggttataa
     9841 aagtgagttt ttgtcatacc gagtttcttg gcggtttcat tgattcgttt aataaaggcg
     9901 tcaggattgt tttgtgataa gtggttagca atcataatag ttgctacatt agatgacggg
     9961 acagccgtca tagtaatcag ttcacgaata ggataagcaa ccccagcatg aatattgtta
    10021 ttactaattt cataaatctt acttatggct tggtctgttt ctgttgcagt cactgtggtg
    10081 ttgaggtttg tttttccttt agctaagtct tcaaatagta aatataaagt aaacatttta
    10141 gacatgctag cgggatcacg ttctaaatcg gcgttatctt cccacaaaat atgaccttta
    10201 ttgtcaataa cgatagacgc tttaggttta ttaatatctt taacatcata tccggcttct
    10261 cgggtaattt gcataatatc tttatgttct tcagcttgca cagagtttac acaagtcagg
    10321 gataataaaa tgcagaggaa agatacgatt aatttaggca taggtaactc ctgaaaactt
    10381 tttattaata ttataacaaa aaatcactaa taaaaaagac atttcattag tgatttttaa
    10441 ttttatatat tctcctaaac ctagctaaag aaactgctgt gcagtaactc cttgtgtcaa
    10501 agggagatta ctattaaagt ttttcgttaa cgtagcgcac aaaatgattc caccacactt
    10561 tgaggaagaa actgcgctca atactatctt ttgccactaa atgaacgcta ggttccttta
    10621 tgagatagcc ttttccaacc ttattaaggt caacgtaata agctttccct aggttctctt
    10681 tttttgtaat aggagcagta agctcttttt taaagttaat ttttaactga ttttgtttag
    10741 tgatattttt cttctgtact actttcaaat cgtttttagc aacagcgatg accgattgtt
    10801 cttttccgtc tctcacactt gcttctttac ctttatatgc agctccttta gctaatacaa
    10861 ggttaggttc gtaggtgttt gtgatatagt tcaagagaga gttagttgct gtaaagcgag
    10921 catatttgtc tttatcggcc ttatcagcat gcataataac agtgagtagt ctcattccac
    10981 tttcagtaga tgtagcaata aaagattggc cagctaattc cgttgtccca gttttcaaac
    11041 ctgtaatacc tggtctaaag acaggcatat ctggtagcat gtagttataa gaatgcataa
    11101 tatctttatc aaatttagca acggacttac tagtaatctt aagaatggaa ggatattcgt
    11161 tgaccaaatg gtaggcagca atagcaatat cacgtgcact cattttattt tcgtcgtttt
    11221 gtgacgattt tggataaatg tgattgccta acatactatt atttaagcca gaagcattga
    11281 ctaggtggct atcatgaatt ccccactttt ccaattgagc agtcatttta tcaacaaatt
    11341 tactttcagt tcctgaaata tgttcagcta aagcaatggc tgcactgtta gcactagaaa
    11401 tcatggcagc gtccacgagt tgtttaacag tatagcgcct tttttctaaa ggaacattac
    11461 tagcatcaga ttcgcgtgtt agttgataag ggtagtcaga tatatttact ttggtattcc
    11521 acttgaggtt accgttatca atttctttat agaccatata aacggtcatt attttagtca
    11581 aggaagcaat agcagcgggt ttgttagcat ctttttcata caagattttt cctgaatcta
    11641 aatcaactgc taaggcatgt ttagcggaag cattaaaaga ttcagcagag atggttggca
    11701 taaaaaaaat gcaactaagt aatagaatag aagtaataat ttttttcaaa ataacacctt
    11761 tagaaatttt ttaa
//
LOCUS       DATROY010000060        11276 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.061, whole genome shotgun sequence.
ACCESSION   DATROY010000060 DATROY010000000
VERSION     DATROY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            36..428
                     /locus_tag="VBD11_001215"
     CDS             36..428
                     /locus_tag="VBD11_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6954170.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPIPGSIIGLILLFLALKY
                     KIIRLRHIDTVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            409..1104
                     /locus_tag="VBD11_001216"
     CDS             409..1104
                     /locus_tag="VBD11_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6954171.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIDDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            1173..1796
                     /locus_tag="VBD11_001217"
     CDS             1173..1796
                     /locus_tag="VBD11_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6954172.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(2065..2136)
                     /locus_tag="VBD11_001218"
     tRNA            complement(2065..2136)
                     /locus_tag="VBD11_001218"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2101..2103),aa:Arg,seq:ccg)
     gene            complement(2209..3411)
                     /gene="rpsA"
                     /locus_tag="VBD11_001219"
     CDS             complement(2209..3411)
                     /gene="rpsA"
                     /locus_tag="VBD11_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6954173.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(3537..3608)
                     /locus_tag="VBD11_001220"
     tRNA            complement(3537..3608)
                     /locus_tag="VBD11_001220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3574..3576),aa:Gln,seq:ttg)
     gene            complement(3614..3693)
                     /locus_tag="VBD11_001221"
     tRNA            complement(3614..3693)
                     /locus_tag="VBD11_001221"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3657..3659),aa:Tyr,seq:gta)
     gene            complement(3754..3984)
                     /locus_tag="VBD11_001222"
     CDS             complement(3754..3984)
                     /locus_tag="VBD11_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6954174.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(4067..5089)
                     /locus_tag="VBD11_001223"
     CDS             complement(4067..5089)
                     /locus_tag="VBD11_001223"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6954175.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            complement(5203..7662)
                     /gene="parC"
                     /locus_tag="VBD11_001224"
     CDS             complement(5203..7662)
                     /gene="parC"
                     /locus_tag="VBD11_001224"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6954176.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            complement(7796..9745)
                     /gene="parE"
                     /locus_tag="VBD11_001225"
     CDS             complement(7796..9745)
                     /gene="parE"
                     /locus_tag="VBD11_001225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6954177.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            9859..10497
                     /gene="plsY"
                     /locus_tag="VBD11_001226"
     CDS             9859..10497
                     /gene="plsY"
                     /locus_tag="VBD11_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6954178.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            complement(10563..11216)
                     /locus_tag="VBD11_001227"
     CDS             complement(10563..11216)
                     /locus_tag="VBD11_001227"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6954179.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
BASE COUNT         3490 a         2248 c         1806 g         3732 t
ORIGIN      
        1 ttcagagtag tagactataa tggaaggagt ttttcatgaa attatacgtt caattaatgg
       61 ttattttaac cttttcattc gctggtgagg ttctatctac tatattcaac ttacctatac
      121 caggtagtat tattggatta atattactat tcctagcgtt aaaatacaaa atcatcaggc
      181 tcaggcatat tgatactgtg gggaattttc tattagcaaa tatgaccatt ttatttttac
      241 caccagcagt tggactgatg gagcactttc aagacataaa gccctatctt tttgggatag
      301 ctatcattat cttaggagcg ctatttctca atattctaac tattggactg gtatcccaat
      361 ggatcaaaaa gagatatgaa ggagattatc cagaaattgg aggtaaaaat ggcaacttta
      421 acgaataacc ctatctttgg tattatgtta accgtatggg cgtattacat tggcataagg
      481 atttttagaa aatatccttc ccctgcaaca actccacttc ttttggcgac tattttatta
      541 attgcttttt taaaattaac acatatctcc tataaagatt actacaatgg tggttcattt
      601 ctaacgatgt taattacgcc ttcaacggtt gtattggcta ttcctctcta tagaaccttt
      661 catttaatga agcatcacat taaaagtatt agcattagca tcatcttagc ttctgttatc
      721 aacacagttt ttaccgcaat agttgctaag ttttttggta tgaaatattt cttagcaatc
      781 tcacttttcc caaagtccgt aactacagct atggccgttg gaattacaag taaagcaggt
      841 ggattagcta ctatcactct ggtcgtagtt gttattacag gcatcttaac aagcgttttg
      901 gggcctattt ttctaaaatt attaaaaata gatgatcctg ttgctatagg gctcgcgcta
      961 ggaggaactg ggcatgctat aggaactgga caagccttaa aatatggcca agttcaaggc
     1021 gccatggctg gactagcaat tggtatcaca ggaatttgct atgttattgt cagtccttta
     1081 gttgcaggct taatattgaa atgaggaaaa gaaaagtaaa cccaaaataa ttatgttata
     1141 atatttaaaa atagaattta ggataaataa ttatgatacc gttaaaaaaa ataactgttg
     1201 gaacgattgt ctgtctctca tttttgggtt taacagcatg ctcttcttca aatactcagc
     1261 aaacctctac ttcaaaatca aatgtatcac aacataaaaa tatcaaagct gaccacgaag
     1321 aattacgttt gaaatttaat aaagtaaagt taggggttaa agcaaataac tttaaaggag
     1381 gcactagtct cgctgaatta aaacaattat ttggtggcga accaaatgaa aaatttgata
     1441 caccagcggg aaatgtcact cttaaaggct atcgttggaa tgtcgatgat atctccatca
     1501 ctatacaatt attaaacgat agtagcattg ttcgttcaat tagtaatttt aagtttattc
     1561 gtgatgccaa catcacaaca aaagattata atagtttaaa aaatggcatg tcatacaata
     1621 aagtaaaaga acttctagga gaacctgatg atatttctca agcagtttct tcagataaag
     1681 aggaactaca ggctgcatgg attagtggca tccagtcgtc agacagtgac ccgggaatta
     1741 accttacatt tgaaaatgat aaactcacta acaaacaaca gcatggttta aaataaaaag
     1801 gcattaacgc ctttttatta ataccttaat tacttattag aaacatttta ttacacaatg
     1861 gtttaatctt agtaccttaa cttagcccct taatggtcat actaactaat tatgatacta
     1921 acttacacag atttcaaact gattctccct tatttgtcaa ccctagtttt acttatcaga
     1981 aatgaggaga atcagtctgt gaggcaaggt tggagataaa ctaatataaa gcctataaca
     2041 taaagcttta tggagcatct attatccatc ttaagggaat cgaaccccta tcccaagaac
     2101 cggaatcttg tgtgatatcc attacactaa agatggtagt acatgttaca ttatagcata
     2161 ctttatcaac aaaaacaaaa agacttgctt tagcaagtct tttaattatt ataattcaat
     2221 atcaccgaat aagtcagcca tagagaatcc agtttgtgtt tctggaagct catagtcgcg
     2281 tttttcttga cgacgtggac gacgtggacg tgattgacgt ttttcttctt tttcaccttc
     2341 tgcttgcgct ggacgctctt caagagcttt cattgaaagt gatacacgtt ctgcatctga
     2401 attaacttca agaactttaa cagtaacttc ttgtcctgca gataatacgt ctttaggatt
     2461 ttcaacacgt ttgtgtgaaa tttgtgaaat gtgtacaagt ccatcaatgc caggtaacac
     2521 ttcaacaaaa gcaccaaagt cagtcaagcg ttttacttta ccttcaataa catcaccagc
     2581 agcaagtttt tgttcaacgc catcccatgg gccaggtgta gttgctttaa gtgaaagtga
     2641 tacacgacca gcttcttcat caattgaaag tactttaact tcaacttctt caccaacagt
     2701 tacaactgat ttaggtgaaa cattgcgctc atgtgataat tcagttacgt gtacaagtcc
     2761 atcaacacca ccaaggtcaa tgaaagcacc aaagcttgtt aaacgagcaa ctttaccagt
     2821 tacaactgaa cctacttcaa tgtttgagaa aacttcttta cgtgcagcag ctgctgattc
     2881 ttcaacaact tcacgacgtg aaaggatgaa gcgattttct gctgcatcaa cttctttgat
     2941 tttagcatca aattcttgtc caacaaattt ttcagtgtta cgtacaaaac gagtatcaat
     3001 cattgaagct gggatgaaac cacgaagtcc ttcaaattca actgaaagtc cgcctttaac
     3061 agcacgtgta cctttaacag taacaacttc accttcacga ccaacaagtt tgtcccatgc
     3121 tttacgagct tccaagcgtt ttttagatac aaggaaagtt actgtatcag tatctttacc
     3181 tacaacttga cgaagaacaa gtacttcaac tgtgtcgcca gctttaacaa aatcattgat
     3241 atcagcatca cgatcgttag tcaattcacg aagtgtcaag acaccttcaa caccagtacc
     3301 atcgataaca acgtttgctt gaccgttatc aactgttaaa acttccgcag tgacaacgtc
     3361 accaggattc acttcgctaa cactgtttag caaatcttca aattcattca ttctaaaaaa
     3421 tcctccaaca aaagctagcc gagctagctt ttgataacaa tatattttct caaggtaacc
     3481 cgcaaggaca acaaatatct ctttgacggt gtcaaacaaa agtttgatac ctttgactgg
     3541 ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct
     3601 ataccccaaa taatggaaag agaggattcg aacccccgaa cccgaaggag cggatttaca
     3661 gtccgccacg tttagcctct tcgctatctt tccgtaacaa cactgactat tctaccacag
     3721 atgttgttag cacgcaagct ttttaaataa atattagcga ttaagattat attcttcaat
     3781 aatatttccc atagcttgat ctaaattttt atagaatcca ctcacttgct cacctttaat
     3841 cacagcaaat ttagaatcta gctctgcaat ttgacctaca attttttttc caataacgag
     3901 ttggtatcca tcaaccttag ttccattaac tgttacttga gcgtctaaaa tttgaatttc
     3961 aattttctta tcttttttac tcactattaa ctccaatatc tcttttattg ttttttattg
     4021 tacacaaaat actaaaagct tgcaagagca agcttttagt atagacttaa tctactttat
     4081 aaatccaccc ttctggtgct tcaacatcac caaactggat ccctactaac tcatcatata
     4141 gtttacgtgt cgctggacca acttctgtct cactgtagaa gacatggaag tcatctccgt
     4201 tttgaattcc tccaattggt gaaataactg cggctgtacc acatgcgcca gcttctgtga
     4261 atttatctaa ttcgtcgaca aagacatctc cttcaatcgc ttccatacca aacctttctt
     4321 tagcaaggta aagcaatgaa tatttagtaa ttgaaggtaa aattgaagga ctcaaagggg
     4381 tgataaactg attatcttta gtaattccaa agaaattagc tgctccaacc tcttcaattt
     4441 tagtatgtgt cgctggatca agatagataa catctgaaaa ttgtttctca tgagcgtatt
     4501 tcccaggaag aagactagca gcatagttac caccaacttt tgcagctcct gtaccattcg
     4561 gtgctgctct atcatattct ttggaaacaa taaagttagt tggtgttaaa ccacctttaa
     4621 aataactacc tactggcatt gcaaataccg taaaaatata ttcttcagca ggtttaactc
     4681 caataatatc tcccacacca attaataagg gacgaatgta taatgttgcc cctgtaccat
     4741 acggtggaac aaactcctca ttagcgcgta caacagattt aaccgctgca ataaatttgt
     4801 ctgtcggaac atgtggcatt aaaagacgat ctgctgttct ttgaagtcgt tctgcatttt
     4861 gatctggacg aaataactga atagagccat ctttagttct ataagctttc aatccttcaa
     4921 aagcttgttg cccatagtgg agggcaggag aactttctga aatatgtagc gttgcatcat
     4981 ctgtcaactt gccatcatcc catttcccat ctttaaaatg cgaaatgtat cggaatggta
     5041 attttctgta agcaaaacct aaattatccc aatctaaatt tactgtcata aggtacctct
     5101 ttctttgggt ttctaactat tgtacgctat tatcattatt ttttcaatat tatttgtaaa
     5161 attcagataa attaccaaac tagtaaaaac acaaatatgt ttttacttaa ttttaactga
     5221 gaatacttct tcgtcagaaa tcttatcaga tacaaaagag ccattacttg tacgttcaga
     5281 aaaactccat tgacttaaat caacatcgta aacttttcct gttttagaca taatctcaag
     5341 tacgaaagta tctccttcat gattcgatac tgaactaaat aaatcaaatt caaaagatga
     5401 atcctctaaa tatacaggtc ctgctttaaa aacgcgatgc ggtttggatt tcaattcacg
     5461 aagaacttga agtcctcgat tagcacgact agttgtgggg ataacatcaa tggccattcg
     5521 tttaagactt cctctatgag ttaataaata taaacttgtg gtgttagcta taaatgcaga
     5581 tacgatgtga tcactatctt ttaaattcat cgcttttaca cctgcggctt tactaccaac
     5641 gactggtaca tcatttatac taaatctcaa cacataacca ttataagtga caagtataac
     5701 atcctctaat tggataggag caacagtcac aacgtaatca tctacatttt ttaacttagc
     5761 atatttagct gtttttgact tatatgtccg ccatggactg atttcttgac gttcaaaacg
     5821 ctttatttgc cctaaactag tgacagcaaa ataagtttct ttagtaaaat catctactaa
     5881 ttcagcataa ataatttctt catttgaagc aaagttaaca agattttgtg ataaatgttc
     5941 accaacgtct ttccatcgaa tatcagctaa ttcatgaact ggacgataag caagattacc
     6001 aaggttagta aacatcaata aatgttgggt agtctttgca ttgctcacaa atatcaattc
     6061 gtcatcttct cgcttaccaa gttcatcaac ggttgatgca ttgaaagagc gagggctggt
     6121 gcgttttaca tatccgcctc tagtaacact gacgtatgtg tcctcttcga taattaaact
     6181 tgcagtatcg atttcaatag tttcagctaa ttcttgaagt tcagaacgtc tagtattggc
     6241 aaatttcttt ttgacctcac gtaactcacg tttcattacg ttatacatcg tacgttcatc
     6301 actaataatt gccttgagca ttgtgatttg ttgacgaagt tcttcttctt cttctctcaa
     6361 cgtcacaata tcagtatttg tgagacgata taattgcaat gtgacgatag cctctgcttg
     6421 agcttctgaa aactcatagc tgaccttcaa attctctttc gcatcagctt tattctcaga
     6481 agcacgaatt aatgcaatca cttcatccaa aatagaaaga accctaatca gtccctcaac
     6541 aatatggaga cgtttttcgg ctttttcttt atcaaatttt gaacgagcaa taataatttc
     6601 tcttcggtga gcgatatagc tagttaatat tcttgataga cccacctgct taggggtata
     6661 gtcatcaata gcaaccatat tgaaattata attaacttgt aggtctgtat atttaaaaag
     6721 ataatttaag acaattgttt catcggcttc ttttttgagc tcaatagcga tacgaaggcc
     6781 atctctatca gactcatcac gcacttctgc tattcctgga actttattat taacacgaac
     6841 atcatcgatt cgttttacta aaactgactt attaacttca taaggaattt cagtaacaat
     6901 aatttgtttc ttaccacctt ttaaggtttc aatagcagtt cgcgagcgaa ccgctactct
     6961 ccccttacca gtctcatatg ccttacgaat ttcatctttt ccttgaatga tagcgccagt
     7021 tggaaaatct ggaccaggta gaaattccat taatttatct aatttagctt tagggtgatc
     7081 aatcatgtac acgacagcat caatgacttc tgctaaatta tgtggtggaa tatctgtcgc
     7141 atagcctgca gatattcctg ttgctccgtt gactaaaaga ttaggaaagg cagctggtaa
     7201 aacagttggt tctttctctg tatcatcaaa attccacgca aagggtacag tattcttatc
     7261 aatatcttga agaagataat cagcaatttc tgatagacgg gcttccgtat aacgcatagc
     7321 tgccgcagga tctccatcca tagaaccatt atttccgtgc atttcaatca atgtctcacg
     7381 atttttccaa tcttgagaca tacgaaccat cgcgtcatag atagaggaat ccccatgagg
     7441 atggaaattc cccataacat taccgactga ttttgcagat ttgcgaaatc ccttttcaaa
     7501 agtattacca tctttattca ttgaataaag gattcgacgt tgaacaggtt ttaagccatc
     7561 acgaatatcc ggcaacgccc gttcttgaat gatatactta gaatagcgcc caaaacgctc
     7621 tcccataatg tcctcaaggg acatattttg aatattagac atatttttaa ttttctccta
     7681 gttggagtgg cttagtatta tttttccact tatttgaaat gaatgattgg ctctcttgtc
     7741 taacgcatac aatattactt gtacactata aaacatgaat aaccaaggct ctttattaaa
     7801 atactgtatt ctcttctaaa gtaaacttga cattatcttc aatccattgg cgtcttggag
     7861 gaactttatc acccatcaag acattaactc gacgttctgc tcttgctaaa tcttcaattg
     7921 ttacacgaat taatgtcctt gtctcagggt tcatagtggt ttcccacaat tgatcggcat
     7981 tcatttctcc aagcccttta tatcgttgta atagtgagcc tttaccaaat ttttgtcgta
     8041 attcttctaa ctctatatcc gtccaagcat actcaacaat ttctttctta ccttttcctt
     8101 ttgacatttt atacaaaggt ggcaaagcaa tatagacatg tccctcttca acaagaggtc
     8161 tcatatagcg ataaaagaaa gttaataata gtgtctgaat atgagctccg tcagtatcag
     8221 catcggtcat aatgataact ttatcataat taatatcgtc aagattaaaa tcaggaccaa
     8281 cacctgcacc aatggtatgg atcattgtat taatttcttc gttttttata atatcagcca
     8341 tctttgcctt agctgtattc aatacttttc ctcgcaacgg taaaatagct tgaaactttc
     8401 tatcacgacc ttgcttagca gagccacctg cagagtctcc ttcgactaaa tagagttcat
     8461 ttttcttagc attttttgat tgtgcagggg ttaattttcc agaaagtaga cctttatctt
     8521 ttttcgactt tttcccattc ctactttcat cacgtgcttt acgtgcagct tcacgcgcat
     8581 cacgtgcttt tattgcctta cggattaaat tagatgctaa atctccattt tccataagaa
     8641 agtaagttaa cttttcagaa acaataccat caacaattgg tcgtgctaag ggacttccta
     8701 gtttatcttt agtttgacct tcaaattgca agtgctcctc aggtacaaga atagataaaa
     8761 tggctgatag cccctcacga taatcagaac cttcaaggtt tttatctttt tctttaagaa
     8821 gtcctgtttt acgtgcatag tcattcatag actttgtaat agctgatttc aagccagttt
     8881 catgtgttcc accatcttta gtacgaacat tattgacaaa agaaaggata ttatctgaaa
     8941 aaccatcatt atactgcaaa gcaacttcga tatgaaattc ttgttcttca ccttcgaaaa
     9001 acatgattgg tgtcaacgtt tctttatctt catttaaata ctctacaaaa tcttgaacac
     9061 cgttttcata atgaaattct agatgctctg cttcttctga acgattatct gttaaggtta
     9121 aagtaacatt ttttaagaga aaggcagatt cctttaagcg ctctgcaatt gtattaaact
     9181 taaaatctat cgttgaaaat actgattgat ctggcataaa gctgacagaa gttccagatt
     9241 ttgattttgg tgccgtgcca atttttttca gagttgtaac gggcttacca ccattttcaa
     9301 aacgttgtct gtaaatagct ccgtcgcgaa tgatttcaac ttctaaccaa gaagacaaag
     9361 cattaactac agatgatcca acaccatgga gaccacctga tgttttataa ccaccttgtc
     9421 caaattttcc acctgcatga agcaccgtga agatgacttc aacagtaggt tttcccatag
     9481 catgcattcc agttggcata ccacgtccat ggtctgtaac agttatactc ccatctttgt
     9541 taataatcac atcaatgcga tttccaaaac ctgatagagc ttcatcaact gcattatcta
     9601 caatttccca cactaaatga tgtaaaccag ttccatctgt ggaaccgata tacatccctg
     9661 gacgcttacg aacagcatct aatccttcta agacttgaat agcatcgtcg ccataattcg
     9721 tcactgttat atcttgttta gtcaaagtga cctccaaatt ttttcatctt ttttatatta
     9781 caagtttttt gctgtttttg caaaattatt tttttaaaaa tagcaaaaag acttttatgc
     9841 ttatgttata ataagggtat gaatattata attatgatta tcattgccta tctgttaggc
     9901 tctatccaaa ctgggctttg gattggtaaa tatttttacc aagtcaatct tcgtcaacac
     9961 ggtagtggta atactggcac cacgaacacc ttccgtattt taggtgtaaa agctggcatt
    10021 gtcactttga ctattgacat tttaaaaggg actctagcaa cacttattcc tatcatatta
    10081 ggtgtaacga cagtatctcc attttttatc ggtttttttg ctattatagg tcacactttc
    10141 cctatttttg cacaattcaa aggtgggaaa gccgttgcta caagcgctgg ggttttgcta
    10201 ggatttgcac cttctttttt cctttactta ttagtgatct ttttattaac gctttatctt
    10261 tttagcatga tttcgctatc cagtattaca gtagctgtcg taggtatcct tagtgtctta
    10321 atttttccat tagtaggttt tatattaacg gactatgact ggatatttac cactgtggtt
    10381 atcttaatgg ccctcacgat tattatccga catcaggata atatcaaacg tattcgaaaa
    10441 aggcaagaaa atttggttcc gtttggctta aacctaagta aacaaaaaaa taaataataa
    10501 aaaaccgcta ctagcggttt tttattataa gactaacaat gtttcgagag tagatttccc
    10561 ttctaattta accaatcaat ctcatttatt ccttcttctt ctaagaaatg atttgttcta
    10621 gaaaaaggtc tgctaccaaa gaaaccacga taagcagata ggggactcgg atgaggactc
    10681 tcaataatat gatgaatagg attatcaatt agcgactttt tctttcgagc atagccaccc
    10741 cacagaataa agactactgg ggtttccttt tggttaacca ctttgatgac agcatcagta
    10801 aaaggttccc atataagccc agcatgacca ttagcttggt gttctggaac tgttaaacaa
    10861 gcattcaata gcagcactcc ttgttgcgcc catgatgtta agtcatgatg tgaacgtgac
    10921 ccaatatctt ctgctaattc ttttagaata ttttgaagtg aaggaggagc tggtaaattg
    10981 tctggaacag aaaatgagag tccttgagct tgttgagggc catggtaagg atcttggcca
    11041 ataatcacta cttttacgtt ttctagtggt gttatctgta tggcattgaa caccttatct
    11101 cttggaggat aaacgattcc gctttcgtaa actgcatcca taaatgtatt aattttattg
    11161 tagtaatgat tgggtagttc tctttttatt aaatcatgcc aactggaatg tttcatttat
    11221 aactccttac ttttagatat ctgtattact agtatagcaa tattttataa aaaacg
//
LOCUS       DATROY010000061        11263 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.062, whole genome shotgun sequence.
ACCESSION   DATROY010000061 DATROY010000000
VERSION     DATROY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            116..1081
                     /locus_tag="VBD11_001228"
     CDS             116..1081
                     /locus_tag="VBD11_001228"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6954180.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(1128..1676)
                     /locus_tag="VBD11_001229"
     CDS             complement(1128..1676)
                     /locus_tag="VBD11_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6954181.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(1743..2528)
                     /gene="codY"
                     /locus_tag="VBD11_001230"
     CDS             complement(1743..2528)
                     /gene="codY"
                     /locus_tag="VBD11_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6954182.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(2654..3865)
                     /locus_tag="VBD11_001231"
     CDS             complement(2654..3865)
                     /locus_tag="VBD11_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6954183.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            4132..4584
                     /locus_tag="VBD11_001232"
     CDS             4132..4584
                     /locus_tag="VBD11_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6954184.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(4639..6021)
                     /locus_tag="VBD11_001233"
     CDS             complement(4639..6021)
                     /locus_tag="VBD11_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6954185.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            6090..7052
                     /locus_tag="VBD11_001234"
     CDS             6090..7052
                     /locus_tag="VBD11_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6954186.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(7087..7965)
                     /gene="aroE"
                     /locus_tag="VBD11_001235"
     CDS             complement(7087..7965)
                     /gene="aroE"
                     /locus_tag="VBD11_001235"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6954187.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(8063..8977)
                     /locus_tag="VBD11_001236"
     CDS             complement(8063..8977)
                     /locus_tag="VBD11_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6954188.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKEWYDIYRSAGNILP"
     gene            complement(9268..>11263)
                     /gene="recG"
                     /locus_tag="VBD11_001237"
     CDS             complement(9268..>11263)
                     /gene="recG"
                     /locus_tag="VBD11_001237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6954189.1"
                     /translation="SNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFKSKSVFDL
                     VDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLADKIELGQE
                     VAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFEINAHLEL
                     KENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMNLQVLKAE
                     NKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNRLLQGDVG
                     SGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILTSGMKAAV
                     KRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIFREKGENP
                     DVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVLEWVKGEL
                     QKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKNDEKDAIMQ
                     DFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRGYKQSYAV
                     LVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQVADIVED
                     YPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
BASE COUNT         3667 a         2218 c         1777 g         3601 t
ORIGIN      
        1 aagtaaaaaa agatactatt ttaaaaataa tcatactata tatttgcttt tagacatatc
       61 ttttgttaaa ctttcttcga aaacgttttc gaatataagt atcggaggtt gtaatatgac
      121 aattaaaaat ctcactgttg caggaagtgg cgttctaggt agtcaaatcg cctttcaagc
      181 agcttacaaa ggaatgtctg tcaccatata tgacattaac gatgaagctt taaataaggg
      241 aaaagaacgc atcaaaaaat tagctaaagt ctatcaatca gaaattgaaa cagcaaaaga
      301 agcatatagt gataaagcta aaagtatcaa gtataataaa aacttattgc ctagcttaga
      361 ccatattttt ctttcaaaag ttgccgattc attagacttg attgctgatt taccaaacca
      421 aattactttt tcaaaaaact tagaccaagc tgtaagtgac gccgatttag taatcgaagc
      481 tgtcccagaa acagttagta taaaagaaga tttttacaaa caattagcta aagtggcacc
      541 ttcaaagaca atctttgcaa caaattcttc aacacttgtt ccaagtcagt ttgctgatat
      601 aacaggacgg cctgacaaat ttttagcaat gcatttcgct aacaatattt ggcaaaataa
      661 tattgttgaa attatgggac ataagggaac tgacgacgag gttgtcaaag aagctttggc
      721 attctcaaaa gatattggta tggtaccttt atatattcat aaagaacaac ctggctatat
      781 tcttaattca atactagttc cattcttaga gagtgcatta gcattgtact ataataaggt
      841 ttctgattct gaaactattg ataaaacctg gaagttagga acaggagcac cgatgggacc
      901 tttagaaatt ttagatatta ttggcatcga taccgcttat aatatcatga agaattattc
      961 agataccacc agcgatccta atagtttaca tgctcacctt gcaaaaatgc ttaaagaaga
     1021 attcattgat aaaggacgta ccggtaaagc tgctggacac ggcttttatg attacgacta
     1081 aactataaaa gaggttagat aaaaatctaa cctcttttat aattcaacta aagaatagtt
     1141 gcaccaagaa cagttttaaa atgatttaaa gcccattgat gattactatc atttaagctg
     1201 gctacagcag ctgctgggac ctcaatttta taacctaagt tataagcatc aattgctgtg
     1261 tgtaatacac aaatatctgt cagtactcct gtcaatatca aagtatctac tcgtctttca
     1321 cgtaggcgaa tatctaaatc agtaccagaa aaggcagaat aatggcgctt atctatccaa
     1381 aaaacacgac tatcttcctt aatagtttca tatagagttc ccaaaggacc atataggttt
     1441 ctaccagaag tccctttaat attatgctca ggaaaaagat tggtctctgg atgaaaaaca
     1501 tctccaatat catgattatc aatcgcaaaa aaaatataat cccctgaccg gtatgctttc
     1561 tctgtaacgt cagctatagc tgaagcgata gattgcgctg gtttacctgc cgttaatttc
     1621 ccatcatctg ccacaaaatc atatgtgtag tctattgaga tgagtgcttt tgtcattttt
     1681 cttcttatct cctcttgatt tcaaaaaagg aactgaaagc tactcccagt cccatgctat
     1741 gattaattat attcttttaa tttgtcaaaa ataccttcgt taataacttt gaggtatgtt
     1801 cccttcatac cgagtgagcg gctctcaatg attcccgcag actccaattt acgaagagca
     1861 tttacaataa cggaacgtgt aattccaata cggtctgcaa taacagatgc cgttaggcgt
     1921 ccttcaagtc catctaattc tcctaagata gcagctacag ctttcatttc tgaataagat
     1981 aaagtgttga tagccattgt caccgctgtt tgtttacgaa tattttcttc tagattttct
     2041 gtttgaaggt taagtaattg aataccaaca acggtgcttg caatttcaac taaaatcaaa
     2101 tcatcgtcgc taaattcttt atcattacgc cagataataa atgttcccaa gcgcattcca
     2161 ccaccataaa ttggagcgat tgttgtaata ccatcttgga agttttcctt tgtctcaact
     2221 gggaaaattg acaaatcatt atctactgat aagttagctt ctgtatcata aacacgacta
     2281 gcagatttaa catagtaatc tgggaattgt tttgtttcaa aaaattcttc cacacggtca
     2341 gtgtttgttt tatatttcat ggcatagcca agtaagttac caccaccatt aataatacat
     2401 gcgttacagt caataatatc agctaattga gccgccattg tattatatgg taattcggca
     2461 tctaagctat ctacggaacg ctgtaagatg gatgtaattt ttctagtttt ttctaataaa
     2521 ttcggcattt ccttcatcct ttgtatgttt tgcgttacaa ttatatcaaa gcattttagc
     2581 ttttgcaaat cgatatcagt acattttaag aacttctatt gttaatttaa tgtttatcta
     2641 ataaaaattc attctagcgc ttgtattggc ttaaaaagcg agccattttt tcttgtagtt
     2701 cagtcaattc atcaacacgt ggtaagtaaa caattctaaa atggtcagcg gtattcatat
     2761 taaatcctct accgtgtgtt agtaatactt tttcttgttt taagaaatta agcacaaact
     2821 cttcatcatt atcaatacgg tacatatctg tatcaatttt agggaaaaga tacagtccag
     2881 catttggttt tacagcagat aaaccaggta tttcattaat tgctttatgg atataattac
     2941 gttgctcgta gatacgacct cctggcaaca acatactatc aatagattgc tgtccgccta
     3001 acgatgtttg aataacctgt tgagcaagaa catttgaaca cagcctcatg ttagctaaca
     3061 tattcaaacc ttcaatataa cctttaacat gttgtcgcgg accagacaat accatccaac
     3121 caacgcggaa accacagatg cgatgtgatt tagataagcc agataaagta actgtaaaaa
     3181 tatcctctgc aatagaagca attgggatat gctccattcc atccataact aaacggtcat
     3241 aaacctcgtc tgaaaaaata atcaagtcat tttgtctagc gatatccaca atctcttgca
     3301 gaatttcacg cggatagact gctccagttg gattatttgg attaatgagg acgattgctt
     3361 ttgttttgga tgtgattttt gatttgatat catcaatatc aggataccaa ttagcttcct
     3421 catcacaaat ataatgcaca gcatttcctc cagcaaggct aacacaagct gtccataacg
     3481 ggtaatctgg cattggaact agaacctcgt catcattatc aagcaaagct tgcattgaca
     3541 tagagattcc ctcactaaca ccattaacaa tgtatatatc atccatgtca acatggatgt
     3601 tttgtaactg gtaatactgc ataacagcct tacgagctga aaaaatacct ttcgaatcag
     3661 agtaaccttc tgattcacgc gcattcgtaa ttaaatcacg aatgacttca tctggcgctt
     3721 cgaagccaaa ggcagctgga tttccagtat ttaaccgcaa tatcttttca ccatttgccc
     3781 tcatgcggtc cgcctcttct aaaacagggc ctctaatatc ataagccaca tgttccagct
     3841 tcattgattt atcaaatatc ttcataaaaa tacctcgtat cttccattat aaaacaaata
     3901 ttctcaaaat tgaaacattt actgcggttt atcaagcgat taagttaata ttaagctatt
     3961 tcagaaaact aacataaaac gctttcattt ttctagaaaa aacgttataa tatagctata
     4021 aaatgaataa ccaacatatc taacttactc atcactcact ccttctgata agatggctag
     4081 ttgagtgcaa gaggttatct cttttggtta tatttagagg aggacaggct tatgtcacaa
     4141 aaatatgaac gcatccttgt cgctattgat ggatcaacag aatctgaact tgcttttgaa
     4201 aaggcagtta acgttgctct tcgcaatgat tctgaattaa tcttgacaca tgttatagat
     4261 acacgtgctc tacaaagctt tgcgacattt gatacttata tctatgaaaa attagaaaaa
     4321 gaagcaaaag atgtccttga ggaatatgaa aaacaagctc gtgaaaaagg agcagataaa
     4381 gttagacaag tcattgaatt tggtaatccc aaaacattac ttgcacatga tattccagaa
     4441 aaagaaaaag tagacttgat tatggttggt gcaacaggtc ttaatacctt tgaacgtttc
     4501 cttatcggat cttcctcaga atacatcctt cgtcacgcta aagtagattt actcattgta
     4561 cgtgacccta ataaaacaat gtaaatttaa tacgctcact ttctaaaata tcctgagtta
     4621 tattaactca ggatattttt attttcgatt taaggatttc tgaatttggc tatctaattc
     4681 acgtcgaatt gatggctcag gagcgaaatg ttcttcccag tcgtctggtt ttaaaatttt
     4741 atgagtaaca ggatcaaaat gagctttgcc atctggaaag attttaccca tattggcctc
     4801 atgtactgta tcaaaaagag gttttgggtc gactcccata agaacaaagg agccataagt
     4861 cagataaagt aaatctgtta aggcatccac ttctcccact aatggtgttt ctggatggtc
     4921 tttagaaata accttatctc ttgctttatc aatagctaag tgtaaattac ggatagattg
     4981 gtcaaatact tcttggttac cttgactagc agcatacaag aactcaacga tttcttctac
     5041 tttaaagtcc gacctaaaac cagcttcctt actgtcatat aagcgtggcg tttcaatagt
     5101 ttcaccatcc attaatagat ggaaagattt tactttattg aaattctcgt cacgactact
     5161 gaatgttttt tcaatctcaa attggataag tccataatgg gctaatgcct tagagatacc
     5221 atcatcatta ttgtcagcag ttgtataacg tgcattttct ttcacttgct gactagcgtt
     5281 ccccatggca acaccaatac caacttgaga taacatctca atatcattat cagaatctcc
     5341 aaatgctatc acttctgata aatcaaaacc atagcattga ccaacacgtt caattccctt
     5401 aacttttgac tgcccttcag aaatgacatc cattgagtaa ggactgcttc ttgttaattt
     5461 aacacgagga aattgttttt gaattcgctc tgattcgccc tctgttgcaa ctaaaacgac
     5521 ctgataaata ggttctcgta agataaccat gaggctatca atattttgag gtttaatccg
     5581 acgaataaaa tgcttaaagc ttcgttcaat cgcttttgcc cattttctcg gaacaaggct
     5641 agatacaatc tgccccaaac ggctagttcc tagaccaata atacctgaac cgagtaatcc
     5701 tgaggctgtt cctaatgaaa tttctcttct tcttgctcca gcataacgaa tcagacgata
     5761 gacagttgtc ttagataatt gattggtata aataatttca cttctactat agatatattg
     5821 accattatag gttacagcaa aatctaaacc aaggttttct aaaaaaggtt gtacaaaact
     5881 aggacctcgc cccgttgcta aaccgactag tattccttgg tctttcaaat ttcgaattgc
     5941 tttaattgtt gacttttgta cattcttacg atcattaaga agtgtaccat caatatcaaa
     6001 aaatactgcc ttaattgcca ctgggtcaac atcccctctt ttttatgata aaataaatta
     6061 tatcacaact tgaaagaagt ataaaatcaa tgaaaaaaat ccttgttcta cacacaggtg
     6121 gaacgatttc catgaatgct aacgagaagg gacaggtcat gtccagcgcc gataacccaa
     6181 tgaaatacgt tgatttatca ttagatgacc ttgatttgac tgttgtagat tttctaaatc
     6241 ttcccagtcc tcaaatcacc cctcatcata tgcttgatat ctatcactat ctgaagcaac
     6301 atgccagtaa ttttgatgga gttgtcatta cgcatgggac tgacacttta gaagaaacag
     6361 catatttttt agacacaatg atactcccta aaatcccaat catcattacc ggtgctatga
     6421 gaagcacaaa tgaacttggt agcgatggcg tttataatta cctatcagcc ctccgtgtcg
     6481 ctaatagtac taaagctgcc gacaaggggg tgctagttgt catgaatgat gagatacatg
     6541 ctgctaaata tgtcacaaaa acacatacaa caaacgtttc gacattccaa acccctacac
     6601 atggccccct tgggattatt atgaaacaag acctgctttt ctttaaagca actgaagaga
     6661 gagttcgttt tgatttagat aaaattacag gaactgttcc tatcgtgaaa gcatatgctg
     6721 gcatgggtga tagtggtatc attagctttt taaattctca aaatattagc ggattagtta
     6781 ttgaagcact cggtgcaggg aatatgcccc caaaagctgc acaagaaatc gaagaattaa
     6841 ttgagcaagg tgtccctgtt gttctagtgt ctcgctgttt taatggtatt gctgagccag
     6901 tttacggata tgaaggtggc ggagcaaaat tacaagaatc tggtgtcatg tttgttaaag
     6961 aactaaacgc tcccaaagcc agactcaaat tactcattgc actcaatgca ggtctcacag
     7021 ggcaaaatct aaaagactat attgaaggtt gataaaaaac tctagaaata tttctagagt
     7081 tttttattaa ttttcagttt gtaatactct ctcaacactg ctaataggca tttcttgatt
     7141 cgttattaat tgaaatgctt ctgctccttg ataaatcagc atccctagac cattataagc
     7201 atgtttcact ccattttgtc taacaaatcg taacaatgct gtttctttag gcttgtaaac
     7261 caagtcgaca actactaaat tcggtgttaa aatttctgga tcgttaatta aactataatt
     7321 atctaatggc ctcattccta aactagtagc atcaatataa aaatgactag ttctaatagc
     7381 gtctttaaat gctgtcttat tttctagata atcaacgact acctttattt gaaactgttt
     7441 tttaatttta tctgataagt caatgacctt atcgtaattt gagctgttac gattaaataa
     7501 tctaatttcc gcaactccct ccatagctgc ttgaactgca actgctttac ctgaaccacc
     7561 aataccagct attgtaatta ttttattttt agcactgaaa ccttgagctg ctaaagcttt
     7621 aaaacaacca atgccatctg tcatatggcc tactaaacgt ccggttccac cttgattaac
     7681 gatagtattt acagcaccca ctaatttagc ttgaggagat aaatcatcta gcaaagggat
     7741 aacactctgt ttaaatggca ttgaaacatt aacaccacga atactcaatg ccctgacacc
     7801 tcgaacagct tctgttaatt taccctcttc tacttcaaat gtcagatagg cataattcat
     7861 gtttttttct tgaaaagagg tattccacat taacggggat agagagtggc gtgcaggatt
     7921 ggcaattaga ccagttagta aagtttcccc atttaatgaa ttcattgtat aaccttcttt
     7981 cttaacaatt tatttcagtt cattttacca aaaactgact caattaaaaa gagagtatgt
     8041 aatttatact ctctccctta gtttaaggca atatattacc tgcactacgg taaatatcat
     8101 accattcttt acgggttaaa gcgatatcat tagccttatc aatcgccttt aaacgagatg
     8161 gattcattga accaacaatc gcctgcattt tggcaggatg acgtaaaatc catgcaatca
     8221 caatagcttc gctgctaaca ttgtagtttt ttgccagttt agctatggtt tcatttaatt
     8281 ccttataatc cggatgatta acaaataaac cacgagataa atcaatttga aatggagacc
     8341 aagcttgaat ggtaactctt ttaagacgac aataatcaac aatgccatca tcatgttcaa
     8401 ttgaagcttt atttaacatg ttaacattta accctgcatc aaacattggt gtatgtgctg
     8461 gcgataactg aagttgattc actgctaagg gctcgtcaag gtaggattgt aacaactcca
     8521 tttgaaaccg gttttgattg ctaacaccaa agtgttttac tttaccttct gccctcagtt
     8581 tggtaaatgc ttctgctact tcttcaggct caactaatac atctggacga tgaagaatga
     8641 gaaaatctaa atattctgtc cctaatcttt ctaaaatacc atctactgat gataaaatat
     8701 actctttaga aaaatcaaag taaccttctc gtatcccaca tttagattga ataatcattt
     8761 tgtcacgatt gacatttaaa tgtttgattg catctcgaaa gcgtaattca gatagccctc
     8821 ccccataaat atcagcatga tcaaaaaaat taatacccaa atctaatgct gttccaacta
     8881 cttcttcagc ttgttttcct ttcaagtcac tcattctcat acaacctaaa gcaattcgag
     8941 ttgcttgaat gcctgtttgt ccaatgtttt gtaacatatg caccccattc taatctgttt
     9001 gtattaacag aaagattaat caattcatta taacaaaatt tctatgaaaa ggcttactat
     9061 tacaaatgca aaaagctgag taaatttaac tcaacttttt ttctgatact cattattgac
     9121 taaataagtc ggtaagaaaa atctattctg aaaagtacta ttataaatta cagtaatcat
     9181 tattgtcata acatacctcc acatctgtga taggaatatc atatgacagc aagcttaaac
     9241 tgagcttagc acagccttaa tagaaactta atcaaaatct gaatgttggc gtaaattatc
     9301 tagaataagt gcccattctg tattttcttt ccaattatta tctttgacaa tatcactagc
     9361 aacacgtctg gcttcttcta aaataggata atcctcaacg atatcagcta cttgaaattc
     9421 tggaattcca gactgacgag taccaaagat ttcaccagaa ccacgcattt ttaaatccga
     9481 ctcagctaaa acgaaaccat ctgtcgtttc tgtcatgatt gtcattcgtt ttttcccaga
     9541 atcagttttg ggattagcca ctaaaacagc gtatgattgt ttatatccac gaccaacacg
     9601 cccacgaagt tgatgtagct ggcttaatcc aaaacgatcg gcatccataa taatcatgat
     9661 cgttgcattt gggacattta cccctacttc aataactgtt gtagatacta aaatatgact
     9721 ttttttatcc ttgaaatctt gcattatagc atctttttca tcatttttca tacgtccatg
     9781 tacaagagca accttagcaa ttccttcaaa ataagtagat aattcagcat gcaatgctac
     9841 tgcattcttt aaatctaaag cttctgactc ttcaatcaac ggtgaaatga catacacttg
     9901 agcatctttt tgcaattcac cttttaccca ttccaacaca gtacctagct gctcgtgttt
     9961 cacccagcgt gtaataatag gtttacgacc ggcaggtaat tcatcaataa tagaaacatc
    10021 catttctcca aaagctgtga ttgctagagt tcggggaatt ggggtggctg tcatcattaa
    10081 aacatcagga ttttctccct tttcacggaa aattctacgc tgtttaacac caaaacgatg
    10141 ttgctcgtct gtaattacaa gccccagttt atggtactgt accgagtcct ggataagagc
    10201 atgagttcct acaatcatat caaccgagcc atttgcaata gctgctaaaa ccgtacgctt
    10261 aacagctgct ttcatacccg aagttaatat agcgattgat aaatctggaa ataactcttg
    10321 cagactaatg tagtgttgtt cagctaggat ttccgttgga accatcaaag ccgattgaaa
    10381 acctgctgta taagctgcat acattgatag accagcaatg actgtctttc ctgatcctac
    10441 atctccttgc aataaacgat tcatatgagc tcccgatgac atatcagata atatttcatc
    10501 taaagagcgc ttttgagcat tcgttagaat aaaaggtaaa gatgaaatct ttgtctccat
    10561 agcatgttta ctataaagaa tagacaaacc atttgtttca gatttatttt cggctttcaa
    10621 aacttgaagg ttcatttgaa agtaaaataa ttcttcaaat ttaatccgac ggagcgcttg
    10681 cttatactct gtgatatctt ttgggaaatg catagctaaa caagcctgac tacgacccat
    10741 caatcggtat tttcccaata aagtagctgg taaattttcc ttcaattcca aatgcgcatt
    10801 gatttcaaaa gctgacttaa tagctttaat caaagtagac tgtgaaattc cctgagatac
    10861 gcgataaaca ggttgcatgt catcttcaac ttgagctaaa accttcatcc cagtaatagc
    10921 tgatttagtt gcatcccatt taccaaagac agctacctct tgaccaagtt ctattttatc
    10981 agctaaatag ggttgattaa aaaaactaac atttaagaca gcttcccctt gacgcaattt
    11041 gaaacttaaa cggttacgtt taaaaccata atattgtaca ttagctggag taacgactaa
    11101 gcctgtaatg actgcttttt caccatccac taaatcaaaa acagattttg atttaaaatc
    11161 ttcatagcga aacggataat aaagaagtaa atcttctact gtataaatat ctaatttctg
    11221 aaatttttct gctgattttg gtccaaatcc cttaagatta gaa
//
LOCUS       DATROY010000062        11236 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.063, whole genome shotgun sequence.
ACCESSION   DATROY010000062 DATROY010000000
VERSION     DATROY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11236
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..834)
                     /locus_tag="VBD11_001238"
     CDS             complement(<1..834)
                     /locus_tag="VBD11_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6954190.1"
                     /translation="MATYRQRGKKKLWDYRIFNEKSELVASGSGFKTKREAMNEAMRI
                     EQQKLLVNSISSDITLYDLWFEWYSLIIKPSNLAETTKNKYFTRGSVIRKLFGNQKVN
                     KIKHSAYQRKLNTYAEKYTKNHVRRLNSDIKKAIQFAKRDGVLLSDFTDGVVIAGRKF
                     VKDADDKYLHSIFDYKKVISYLENNLDYSNSIVYYLLLVLFKTGLRVGEALALTWDDV
                     NFEDLEIKTYRRFSGDKGTFSPPKTKTSIRTIPISQSLALILRDLKDDQQVMLKNLKI
                     VN"
     gene            complement(884..1210)
                     /locus_tag="VBD11_001239"
     CDS             complement(884..1210)
                     /locus_tag="VBD11_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEO6954191.1"
                     /translation="MQQVFNVSVPVPDDVVIISKEEYLNLLSDNEQGKWWDIDNLQEL
                     LGIGRSKLINDILLNPDIKKEVDLSINPNGFIVYPKGKGSRYKILATKARKYFEDNFG
                     SILLNS"
     gene            complement(1403..2035)
                     /locus_tag="VBD11_001240"
     CDS             complement(1403..2035)
                     /locus_tag="VBD11_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954192.1"
                     /translation="MIGDYSVMDWATLGGVVGIIFTNVGVLISNSRDNKAIQRELELL
                     EEGQEKLVDEFSKISTNQYDKYVLIQSNLSNNIEKNKQELVQKNSYVKEDTKYIRDEM
                     LIEKKSKEEVYNHVKNGDKLIEKMAFANELILKFGEVSRENQMLGLKVNSLEEKIVDL
                     SNQPKNDEISKLRKSISSFERELSRFEDVGYSEAEEIKSTLRRILNDLEG"
     gene            complement(2116..2907)
                     /locus_tag="VBD11_001241"
     CDS             complement(2116..2907)
                     /locus_tag="VBD11_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6954193.1"
                     /translation="MSKVKVLSLITVSGLFLMAGNLSASADVVTSGGDTIMLSGVDAG
                     VSDSIMPPPSSINPVTDTTEPSAPTPSTDPITDTTEPSAPTPSTDPITDTTEPSAPTP
                     STDQTTGTTDSSTPSSSTTNPVDGITDNGTKPNAGIDKPSTNKPSDHSESSIKPVTKP
                     TINQPITTVTGDQVIGTQDGKVLVQTPSGTQLKDAAEVGGNVQKDGTVAIKKSDGKIE
                     VLPKTGEGKTIFTIVGLLLIAGAVWIGFKDNIKKYLTFFKKKSEK"
     gene            complement(2924..3472)
                     /locus_tag="VBD11_001242"
     CDS             complement(2924..3472)
                     /locus_tag="VBD11_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954194.1"
                     /translation="MKRRKIVLFLMVLVMAFIVYKVIQKDNPVIKTSGNHTKVERVEP
                     KHKVKSYDSTKEAKSTLEASDRQVSDKEKEKVKRSITVPIDYLVKIPTQSDIKGDYQN
                     KLSITNPTVAQTVKTMLMAGYHFDLESLQVYESDASNVYQFTINLVTDKRDKLSLAGN
                     YVLGTDQFEIASVHGTPVNVVF"
     gene            complement(3472..4845)
                     /locus_tag="VBD11_001243"
     CDS             complement(3472..4845)
                     /locus_tag="VBD11_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6954195.1"
                     /translation="MGILIVFPFLLVLLLTASSSSQQDCQITPDVSSQVETSSENASN
                     SDWTKKGTVANQTAEKVFNAWVSKGLSGASASGIVGWVNSEGGFAMIGRAEGHYGNDL
                     KTNSIAFNVRPTGLSYYTTEAGGGIYQITPFTKYAPLGDPKWEDADAMNAFVMKSIKS
                     GDWNKAMDLSGGNHSFEEMAQMTDPKQATLVWNAYERGNTAYINKAQKTADGQKAYEI
                     FNGSKYKFDAEKFSKSFGGGSKTSSSGKSDIISDSTVLSLCDSASNAVQGLFGKDKSG
                     KVKYTSYNAWKPDQLPSDLKKYAIDPKSVGLFYRTSSGWNAIASTGGQCTDLSASLMY
                     GLWLKGGMHPTQRMGNGNMVVSNWVSAFGGRSENTPSSGAVFSSSGTSSAGHTGVVSH
                     VFENGDMLIVEQNYSTYSGDNRGFGPYSWNYRYVSTLELKKEHYSFYNPSKAGYKLSD
                     KVKTVGS"
     gene            5008..5151
                     /locus_tag="VBD11_001244"
     CDS             5008..5151
                     /locus_tag="VBD11_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEO6954196.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            complement(5341..7341)
                     /locus_tag="VBD11_001245"
     CDS             complement(5341..7341)
                     /locus_tag="VBD11_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954197.1"
                     /translation="MDKIDDGLIASTFAKVKDVDIFALKAYMEITHGAETGAQSILLD
                     VFVNFPFFLLNLIVGLFSVILRFFENFSLYDTYKQTVYHSSQKLWENLSGNGSYTSSL
                     LYLLVAISAFSIFISYLFSKGDFSKRLIHLFVVIILGMGYFGTIQSTSGGIYILDTVH
                     QLAGSFSDAVTNLSLDNPSGGKTKITQKSSVADNYVMKTSYTAYLFVNTGQLNGKFHN
                     NQTGKEEKFDNEQVLGKYDKSGKFITPKQKDILNYTDNLGDKATEGEEKNRWLSAVND
                     YLWIKSGYVILKIFEAVILAVPLILIQLIAFMADVLVIILMFIFPLALLVSFLPRMQD
                     IIFNVLKVMFGAVSFPALAGFLTLIVFYTQTLIATFVKKKFTDGSLLSGSNFKGQAIL
                     FMLLITVFVQGCVFWGIWKYKETFLRLIIGSRASQVINQSVDKINEKAENLGITPKSI
                     YERAHDMSSLAMMGAGYGVGTMMNAQDNWNAFKERQQANLDDGQSKTNDADKYDEANA
                     DDTVISKEAELTNEGEYQSELPKEASKRIEQLGKESSYELSFISEGNSTEEILKNVKS
                     DNHTFQEGDGDTSLTNQDMITNDIENHSNNYTSPLKQRKLNKLEGELSQFNSDVSMTK
                     NHGKNAFEKGFNASKTKEVRKQHNLERQSKVLEELEKLREGK"
     gene            complement(7357..9858)
                     /locus_tag="VBD11_001246"
     CDS             complement(7357..9858)
                     /locus_tag="VBD11_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6954198.1"
                     /translation="MVKKLEYPIDRIHQNLALRKDKVVVAYYRIPNTPITITDSDKKD
                     SHKTKVAQVIKKLAKYKNFDISLIPKDYLLGEKMSDFEADLADDVKELGLETLDFTVD
                     TLTHEMEIPYQFDWLIGVDLGKGQYNANIKEFIYNQFESIASNFASLAGYEVEVDEDW
                     YKEHSEEELLVYSLLSTLKAKRLTDVDLFYYQRMQFLRYVPHTKSEVIANRNMLNVTD
                     TLIKSLEGGFLKLESAYGSSFVSVLPVGRFSTIFNGFHLGELVQRMSFPVELRFKAEF
                     IDKTKLGGTMGRSNTRYDQIMKEAYNTNTVQQDDILMGAYSLKDLMKKVGNKEEIIEY
                     GCYLVVAGSSLNQLKQRRYAILSYFDDMKVNVYEASHDTPYLFQALLYGQDLQKTTRK
                     WNHLVTARGFSELMLFTNTQSGNRIGWYIGRVDNRLTAWDSIDEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHVIITGATGQGKSYLAQMIFLHTAQQNVRVLYVDPKRELRQHY
                     LKVVSDPEYARKFPLRKKQIEETNFVTLDSSVKENHGVLDPIVILDKEGASSTAKNML
                     LYLLKNATEIKLDQTTALTEAISQVIAKREAGEVVGFNQVIEVLIDSESDEVQSVGRY
                     FKAIIQNSILELAFSDGDVAGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERSDDETVEIFDEAWVLMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFSFYEKSEREDILSHVGLEVTPKNLEWIDNMISGQCLYYDVYG
                     NLNMISIHNIHPDIDPLLKPMKKTVSSHLENKYAS"
     gene            complement(9879..10289)
                     /locus_tag="VBD11_001247"
     CDS             complement(9879..10289)
                     /locus_tag="VBD11_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6954199.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEVKTAKGKVIWYFSTPMQLSFFIV
                     FFIILMLMLTVCSPLMQVLNALTHSVSMLLYWFVPSKLAKFYVEYEPQGKKMHIFLWD
                     YLVYLKDFGLNKKGIYQGERVDIEEEIVFEKTKI"
     gene            complement(10300..10566)
                     /locus_tag="VBD11_001248"
     CDS             complement(10300..10566)
                     /locus_tag="VBD11_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954200.1"
                     /translation="MNLVKVNSVILAGGPNVSGLKQFFQGDGIYLISVIAVFIIIKNW
                     KEANWLKVFSTLAIYAIIVSMLKGQQILSFLGGMLRWFGVETGL"
     gene            complement(10587..>11236)
                     /locus_tag="VBD11_001249"
     CDS             complement(10587..>11236)
                     /locus_tag="VBD11_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6954201.1"
                     /translation="INYSQDTADQRKTELENYYSFSTASMTDDVRKSRTLQTQRLISV
                     EKEKDYYIALMRIGYEVDKKSYQMNLAVPFQMQRGLLAIVSQPYTVAEDLYLGKSKAF
                     EKKTLDQVKELSKEQVSSIQKFLPVFFNKYALSNKTDLKLLMKTPELMGKGFKVSELD
                     LNNAIYYQEKKHQVVQLSVTFEDLVTGGTRSENFTLYLFKADNGWYVEEMYHYFK"
BASE COUNT         3618 a         2223 c         1449 g         3946 t
ORIGIN      
        1 gtttacaatt tttaagtttt tcaacataac ttgctgatca tccttcaaat ctcttaagat
       61 taatgctaaa gattgactaa taggaatagt gcggatagaa gtttttgttt taggtggact
      121 aaaagttccc ttatcgccac taaatcttcg atacgtctta atttctagat cttcaaaatt
      181 aacatcatcc caagttaatg ctaaagcttc acccactcgt aaacctgttt taaatagcac
      241 cagtaataaa taataaacga tactatttga ataatccaaa ttgttttcta aataagatat
      301 cactttcttg taatcaaaaa tactatgtag atatttatca tctgcatcct taacaaattt
      361 cctaccagct atgacaactc catcagtaaa atcagataat aagacaccat ctcttttagc
      421 aaattggata gcttttttta tatctgaatt taaccttcta acatggtttt tagtgtattt
      481 ttcagcgtac gtatttaatt tcctctgata cgcactatgc tttattttat ttactttctg
      541 atttccaaaa agtttacgaa taacagaacc tcttgtaaaa tatttgttct ttgtagtctc
      601 tgctagattg ctaggcttta taattaaact gtaccattca aaccacaaat catacaatgt
      661 tatatcagaa gatatagaat ttactaataa cttttgttgc tctatcctca tagcttcatt
      721 catagcttca cgctttgtct taaaacctga accagaagca actaactcac ttttttcatt
      781 gaatattcta taatcccata atttcttttt acctctttgt ctatatgttg ccattttact
      841 cctttcttag acatcaagga atttatcctc aatattatat catttaacta tttaataaaa
      901 tagaaccaaa attatcttca aaatattttc tagcttttgt tgctaatatt ttatatcgac
      961 tacctttacc tttcggataa acaataaaac catttggatt aattgataaa tctacttctt
     1021 tttttatatc tggatttaac aaaatatcat taatcaattt tgaccttcca attcccaata
     1081 actcttgcaa gttatcaata tcccaccact tgccttgttc attatcactt aataaattta
     1141 aatattcttc ttttgaaata ataacaacat catctggtac aggaacactc acattaaaaa
     1201 cctgttgcat actcatcctc cttataactt gcttaatccg aacgttattt gttccaggta
     1261 attaggtttt ccactttaaa accgaactat ttttcagttg cagttttaat ttaaaatcca
     1321 tatcccccga acattattct ttaataaaaa aacaaacaaa aaaagccatg aaattaatca
     1381 caactttatt tctatcaact tcttatcctt caagatcatt taatattctt cttaaagtac
     1441 tttttatttc ttctgcttcg ctataaccga catcctcaaa cctagataat tccctttcaa
     1501 aacttgaaat tgatttacgt aattttgata tttcatcatt tttaggttga tttgataaat
     1561 cgactatttt ttcctctaaa gaattaactt ttaagcctaa catttgattt tctctgctta
     1621 cttcaccaaa cttcaaaatt aattcattag caaaagccat tttttcaata agtttatctc
     1681 catttttcac atgattataa acttcttcct ttgacttttt ctcaataagc atttcatcac
     1741 ggatatattt agtatcttct ttaacatatg agtttttttg aactaattct tgtttattct
     1801 tttcaatatt gtttgataaa ttgctttgta tcagaacata tttatcatat tgatttgtcg
     1861 aaattttgga aaattcgtct accaatttct cttgaccttc ttctaaaagc tcaagttctc
     1921 gttgaatagc tttattatcc ctagagtttg aaatcaaaac tcccacattt gtaaaaataa
     1981 ttcctacaac accacctaaa gtagcccaat ccattactga ataatcccct atcatttttt
     2041 ctcctattct atcacatttt attccaatct gtattattta attaaaaaag ccatgaaatt
     2101 aatcatggca ttatcttatt tttcactttt ctttttgaaa aaggttaaat acttttttat
     2161 attatcttta aaaccaatcc aaactgctcc agcaattaac aagagaccta ctattgtaaa
     2221 gatagtctta ccctcaccag tttttggcaa tacttctatt tttccatctg attttttaat
     2281 tgctacagta ccgtcttttt gaacatttcc accaacttca gcagcatctt ttaattgcgt
     2341 acctgatggt gtctgtacta aaactttacc atcttgagta ccaatgactt gatctcctgt
     2401 tacagttgta ataggttgat taatagttgg ctttgttact ggttttatag atgattctga
     2461 atgatcacta ggtttattcg tagaaggttt gtcaataccg gcatttggtt tagtaccatt
     2521 atcagtaatt ccatctactg gatttgttgt acttgatgat ggagtacttg aatctgtcgt
     2581 acctgttgtt tgatctgtac ttggtgttgg tgcacttggt tctgtggtat ctgttattgg
     2641 atctgtactt ggtgttggcg cacttggttc tgtggtatct gttattggat ctgtacttgg
     2701 tgttggcgca cttggttctg tggtatctgt tactggattt atacttgatg gtggtggcat
     2761 tatagaatca gatacacctg catctacccc tgatagcatt atagtatcac ctccactagt
     2821 tacaacatct gcacttgctg ataaattacc agccattaaa aatagtcctg aaacagtaat
     2881 taaacttaaa acttttactt ttgacataaa ttttccttct ttcttaaaat actacattta
     2941 caggtgtacc atgtacactc gcaatctcaa attggtctgt acctaataca taattaccag
     3001 ctaaacttaa tttgtctctt ttatctgtta ctaaattaat agtaaactga taaacattac
     3061 tcgcatctga ctcatatact tgtaaacttt ccaaatcaaa atgataacca gccatcaaca
     3121 tggtttttac agtttgggca actgtaggat ttgtaatgga taatttattt tgataatcac
     3181 ccttaatatc tgattgtgtt gggattttta caaggtaatc aataggtaca gttatagatc
     3241 ttttaacctt ctccttttcc ttatcagata cttgacgatc actagcttcc aaagtagatt
     3301 ttgcttcttt agttgaatca taagacttaa ccttatgttt cggttcaact cgttcaactt
     3361 ttgtatgatt accacttgtt ttaatcactg gattatcttt ttgaataact ttataaacta
     3421 taaaagccat cactaacacc attaaaaata aaactatttt tctacgtttc attaactacc
     3481 taccgttttc actttatcag acaatttata accagcttta gagggattat aaaaactata
     3541 atgctctttt tttaattcta aagtagaaac ataacgataa ttccatgaat atggtccaaa
     3601 tcctctatta tctccagaat aagttgaata attttgctct actattaaca tatcgccatt
     3661 ttcaaataca tgactaacca ctccagtatg cccagcacta gaagtacctg aacttgaaaa
     3721 aacagctcca gaagatggag tattttcaga gcgtccaccg aatgcagata cccaattaga
     3781 aacaaccata ttaccatttc ccattctttg cgttggatgc attccgcctt taagccataa
     3841 accatacatc aaacttgctg aaagatctgt acattgacca ccagtagaag ctatcgcatt
     3901 ccatccacta gacgttcgat aaaataaacc aactgactta ggatctattg catacttttt
     3961 caaatcactt ggtaattgat ctggtttcca tgcattataa gaagtgtatt taactttccc
     4021 tgacttatct ttaccaaata agccctgtac cgcattacta gctgaatcac aaagagataa
     4081 aactgtagaa tcagaaatta tatcgctctt accagacgag gacgtttttg aaccaccacc
     4141 aaaactttta gaaaattttt ctgcatcaaa tttatatttt gaaccattaa atatttcata
     4201 ggctttttgc ccatccgcag tcttttgagc tttattaata tatgcagtat ttcctcgttc
     4261 gtaagcattc caaactaaag tggcctgctt gggatcagtc atctgtgcca tctcttcaaa
     4321 agaatgattt ccacctgata aatccattgc tttattccag tctcctgatt taatggattt
     4381 cataacaaag gcattcatgg catctgcatc ttcccatttg ggatcgccta atggggcata
     4441 ctttgtgaaa ggtgtgattt gataaatacc acctccagct tcagtcgtat agtaagataa
     4501 cccagtaggt ctcacattaa aggcaattga atttgttttt aaatcattac cataatgccc
     4561 ttctgctcga cctatcatag caaatccacc ttctgaatta acccaaccaa caatccctga
     4621 tgcactcgca ccagataagc ctttacttac ccaagcattg aataccttct ctgctgtttg
     4681 atttgcaact gttccctttt ttgtccaatc actattggaa gcattctcac ttgaggtttc
     4741 aacttgtgaa gatacatctg gtgtaatttg acaatcttgt tgagaacttg acgaagcagt
     4801 caacaataaa accaataaaa aaggaaatac aatcagtatt cccaaacagc ctaatttttt
     4861 cttatgtttt actttagttc cctcctttca aacaaaataa aaaagcacca acaaaaattg
     4921 atactttaaa ccactatgaa atatgatata ataatgaagc agaaaagtta agacggtggc
     4981 tcttatttct gaaaggtagg tggtcccatg ggcaattcat caaaatccaa cagaaaggag
     5041 gagctagatt tgtctacctt tgaggtctta acactcatgt ttatagcagg taacttcgtt
     5101 atcgctctcg tgaagttagt ccttgaactg gtaaaatcga caaaaaaata accgtctcaa
     5161 ctttggcgag ttaacggtta ttttttaacg gttattttct gccaccgtct taaacggttc
     5221 tgctaagcag ttgttagaag caactgcttt ttatttacaa ttatattata cataatctaa
     5281 aaaatagtgt caattaatat atagtttact aaacctctca atgtcataaa actccttgta
     5341 ctacttacct tctctcagct tttctagttc ttccaaaact tttgactgtc gttcaagatt
     5401 atgttgcttt ctaacttcct tagttttact ggcattaaat cccttttcaa aagcattttt
     5461 accatgattt tttgtcatgg atacatcact attaaattga cttaattcac cctctagctt
     5521 attaagcttt cgctgtttta atggtgaagt atagttattt gaatgatttt ctatatcatt
     5581 tgttatcata tcctgatttg ttaatgaagt atctccatct ccttcttgaa acgtgtgatt
     5641 atccgactta acatttttta aaatttcctc tgttgaatta ccttctgata taaacgatag
     5701 ctcataagaa gattcctttc ccaattgttc aatcctttta cttgcttctt taggtaactc
     5761 tgattgatat tcaccttcat tagtcagctc agcctcttta gaaataacag tatcatccgc
     5821 attagcttca tcatacttat cagcatcatt agttttagac tgaccatcat ctaaattagc
     5881 ttgttgtctt tctttgaaag cattccaatt atcttgtgca ttcatcattg taccaacacc
     5941 atagccagct cccatcatgg caaggcttga catatcgtgt gccctctcat aaatggattt
     6001 tggcgtaatt cctagatttt ctgctttttc attaatttta tctacagact gattaatgac
     6061 ttgagaagct ctagacccta taatcagtct taagaaagtt tctttatatt tccaaattcc
     6121 ccaaaacaca caaccttgga cgaaaactgt tattagcaac ataaacagta ttgcctgacc
     6181 tttaaaatta ctaccactaa gcagggaacc atctgtaaat tttttcttaa caaaggtggc
     6241 tattaaagtt tgagtataga agactatcaa ggttaaaaaa ccagctaagg caggaaagga
     6301 aacagcccca aacatgactt tcaaaacatt gaaaatgata tcttgcattc taggcaaaaa
     6361 tgaaactaat aaagctaatg gaaaaataaa cattaatata atcactaaaa catctgccat
     6421 aaaagcaatt aactgaatga gtattaatgg aactgcgagt ataaccgctt caaaaatctt
     6481 taagataaca tagcctgatt ttatccaaag ataatcattt actgcagaaa gccatctatt
     6541 tttttcctcc ccttcggttg ccttatctcc caaattatca gtataattca atatatcctt
     6601 ttgttttgga gtgataaatt tacctgattt atcgtactta cctaaaactt gttcgttatc
     6661 aaacttttct tctttacctg tttgattatt gtgaaactta ccatttaact gaccagtatt
     6721 gacaaacaaa taagccgtat aagaagtttt catcacataa ttatcagcta ctgatgattt
     6781 ttgtgtgatc tttgttttac cacctgatgg attatccagt gataaattag ttaccgcatc
     6841 tgaaaacgaa ccagctagtt gatgaacggt atctaaaata taaataccac ctgatgttga
     6901 ttgaatcgta ccaaagtaac ccatacctaa aatgataacc acgaacaagt ggatcaaacg
     6961 tttagaaaaa tctccttttg aaaaaagata tgaaataaat attgaaaaag ctgaaatcgc
     7021 aaccaataaa taaagcaagg agcttgtata agacccatta cctgatagat tttcccataa
     7081 tttttgagac gaatgataaa ctgtttgttt ataggtatca tacaaactga aattttcaaa
     7141 aaagcgtaga ataacagaaa ataaaccaac aatcaagtta agtaggaaaa agggaaaatt
     7201 aacaaaaaca tctaacaaaa tgctttgagc tccagtctca gctccgtgag ttatctccat
     7261 ataagccttt aacgcaaata tatccacatc ttttaccttc gcaaaggtcg aagcaatcaa
     7321 accatcatct attttgtcca tctgattacc tcccttctac gaagcatatt tattttcaag
     7381 gtgacttgat actgttttct tcataggttt taacaatgga tcaatatctg gatgaatgtt
     7441 atgtatcgaa atcatattca agttaccgta aacatcataa taaagacatt gacctgaaat
     7501 catattatca atccattcta gattcttagg tgttacttct aaaccaacat gacttaaaat
     7561 atcttcacgt tccgattttt cataaaatga aaaaattgtc ccaaagccgg tcgtatcatc
     7621 atcgttttct gcatcatgaa ccgattgaga tactagcatc aacacattat acttagagcg
     7681 accaacacga cgcatagact tgataacagc cttaccttct gaggattgca taagtaccca
     7741 tgcttcatca aaaatttcaa ctgtttcatc atcactacgc tcaccaaaat gcttacaaaa
     7801 cgcacctaaa gcaaacatta aagcaatcga attactttca tgatcagata tatgatctga
     7861 accgtcctta ggcaatgata aatccgcaac ttctaaaact gtcacacgtt cttcgtaaga
     7921 aagaccagca acatcaccat ctgaaaatgc caactctaaa atagaattct gaataatagc
     7981 tttaaaataa cgaccaactg actgaacttc atcactttca gaatcaataa gaacttcaat
     8041 aacctgatta aaaccaacga cttcaccagc ttcacgtttt gcaatgactt gactaattgc
     8101 ttctgttaaa gctgttgttt gatctaattt aatctctgta gcgtttttta acaaataaag
     8161 caacatattt ttagccgttg aggaagcacc ttccttatca agaataacaa taggatctaa
     8221 tacaccatga ttttctttga cagaactatc aagagtgaca aaattcgttt cctcaatttg
     8281 cttcttacgt agaggaaact tacgtgcata ctctggatca gaaacaactt ttaaatagtg
     8341 ttgccgtagt tctcgtttag ggtcaacata aaggacacga acattttgtt gagcagtatg
     8401 taaaaagatc atctgtgcta agtatgattt cccttgacct gtcgccccag ttataatcac
     8461 gtgaggattt ttagtaacct taccagcaac atcttcttta ttcgcgactg tcgcattaaa
     8521 taagacaaga tttttagatc ccataatagc ttcgtcaata gaatcccaag cagttaaacg
     8581 attatctact cgtccaatat accaaccaat cctgttacct gattgggtat ttgtaaaaag
     8641 catcaactcc gaaaaacctc gagcagttac taaatgattc cacttacgag ttgttttttg
     8701 taaatcttga ccataaagta acgcttgaaa taaatatggt gtatcatgac tggcttcata
     8761 aacattgact ttcatatcat caaagtaact taaaatggca tatctacgtt gttttagttg
     8821 atttaaacta gaaccagcaa ctaccaaata acaaccatac tctatgattt cttccttatt
     8881 tccaactttt ttcataagat ccttaagcga atatgctccc atcaagatat catcttgttg
     8941 aaccgtattt gtgttataag cttctttcat gatttgatca tagcgtgtat ttgagcgacc
     9001 catagtgcca cccaattttg tcttatctat aaattccgct ttaaatctta attcaaccgg
     9061 aaaactcatt ctttgaacta gttcaccaag atgaaagcca ttaaatatgg tagagaaccg
     9121 acctactgga agaacggata caaatgaact cccataagca ctctccaatt ttaaaaaacc
     9181 accttctagt gatttaatca acgtatctgt cacatttaac atattccgat tagctataac
     9241 ctcactttta gtatggggaa catatcttaa aaattgcatc cgttgataat aaaataaatc
     9301 aacatccgtt aatcttttgg ctttcagggt agacaataaa ctatagacaa gtaattcttc
     9361 ttctgaatgt tctttatacc aatcttcatc tacttctacc tcataaccag ctaaagaagc
     9421 gaagtttgat gcgatagatt caaactgatt ataaataaat tcttttatat tagcattgta
     9481 ttgcccttta ccaagatcaa ctccaattaa ccaatcaaac tgatacggta tctccatttc
     9541 atgtgttaac gtatcaactg taaaatcgag tgtttctaac cctaattctt taacatcatc
     9601 tgctagatcc gcttcaaaat ccgacatttt ttcaccaaga agataatctt ttggtattaa
     9661 tgaaatatca aaatttttat atttagctaa ttttttgata acctgtgcta ctttcgtttt
     9721 gtgactatct ttcttatcac tatctgtaat agtgattggg gtatttggaa tacgatagta
     9781 tgcgacaaca actttatctt ttcgtaaagc taaattctga tgtattctat caatcggata
     9841 ttccaatttt tttaccatct gatctccttt cttagtcttt atattttcgt tttctcaaaa
     9901 actatttctt cttcaatatc aacacgttct ccttgataga tacctttttt atttaaccca
     9961 aaatctttga gataaaccag ataatcccac aaaaagatat gcatcttttt tccttgcggt
    10021 tcatattcta cataaaattt cgctaacttg cttggaacaa accaatacaa gagcattgaa
    10081 acggaatgcg ttaaagcatt taacacctgc attaatggag aacataccgt cagcatcaac
    10141 attaagatga taaagaaaac aataaaaaat gataactgca taggtgtaga aaaataccaa
    10201 atcactttgc cttttgccgt tttaacttcc tgaatccagt aaggagcatt caagcccctg
    10261 ctgtaatcaa aatatttttc ttcttccata acttataact tataaccccg tctcaacacc
    10321 aaaccagcga agcattccac ccaaaaaaga tagaatctgt tgacctttaa gcattgatac
    10381 aataatcgca taaatagcta aggttgagaa aacttttaac caattagctt ctttccaatt
    10441 cttaataatt ataaagacag caattacact aatgaggtaa atcccatcac cttgaaaaaa
    10501 ttgctttagt cctgaaacgt tgggaccacc tgctaaaata acactattca cttttactaa
    10561 attcattttt tccctctctt acacctctat ttaaaataat gatacatctc ttcaacatac
    10621 caaccattgt ctgctttaaa taaatataat gtaaagtttt ctgatcgtgt gccacctgtt
    10681 acaagatctt caaaagttac tgataattgc acgacttgat gctttttttc ttgataataa
    10741 atcgcattat taagatctag ctcactaact ttaaatcctt tccccatcag ttctggagtt
    10801 ttcattaaca gtttcaagtc tgtcttgtta cttaaggcat atttattaaa gaaaactggt
    10861 aaaaactttt gaatagaact tacttgttct ttcgatagtt ccttgacttg atctaatgtt
    10921 ttcttctcaa atgctttaga tttacctaaa tacaaatctt ctgccacagt ataaggttga
    10981 ctaacgattg caagcaatcc tctttgcatc tgaaaaggaa cagccaagtt catctgatat
    11041 gattttttat caacttcata accaatacgc atcaaagcta tatagtaatc tttttctttt
    11101 tcaacactta ttaaacgttg tgtctgcaaa gtcctagact ttctaacatc atctgtcata
    11161 ctggcagttg agaaagaata ataattttct aattctgttt ttctttgatc tgcagtatct
    11221 tgtgaatagt tgatat
//
LOCUS       DATROY010000063        10975 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.064, whole genome shotgun sequence.
ACCESSION   DATROY010000063 DATROY010000000
VERSION     DATROY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(118..846)
                     /gene="lrgB"
                     /locus_tag="VBD11_001250"
     CDS             complement(118..846)
                     /gene="lrgB"
                     /locus_tag="VBD11_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6954202.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(848..1303)
                     /locus_tag="VBD11_001251"
     CDS             complement(848..1303)
                     /locus_tag="VBD11_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6954203.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(1473..2213)
                     /locus_tag="VBD11_001252"
     CDS             complement(1473..2213)
                     /locus_tag="VBD11_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6954204.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(2194..3939)
                     /locus_tag="VBD11_001253"
     CDS             complement(2194..3939)
                     /locus_tag="VBD11_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO6954205.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(3966..4610)
                     /locus_tag="VBD11_001254"
     CDS             complement(3966..4610)
                     /locus_tag="VBD11_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6954206.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(4734..5129)
                     /locus_tag="VBD11_001255"
     CDS             complement(4734..5129)
                     /locus_tag="VBD11_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6954207.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            5210..5926
                     /locus_tag="VBD11_001256"
     CDS             5210..5926
                     /locus_tag="VBD11_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6954208.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(5980..6606)
                     /locus_tag="VBD11_001257"
     CDS             complement(5980..6606)
                     /locus_tag="VBD11_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6954209.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(6639..6962)
                     /locus_tag="VBD11_001258"
     CDS             complement(6639..6962)
                     /locus_tag="VBD11_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6954210.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(6959..7243)
                     /locus_tag="VBD11_001259"
     CDS             complement(6959..7243)
                     /locus_tag="VBD11_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6954211.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            7404..7643
                     /locus_tag="VBD11_001260"
     CDS             7404..7643
                     /locus_tag="VBD11_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954212.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            7828..8895
                     /gene="pepA"
                     /locus_tag="VBD11_001261"
     CDS             7828..8895
                     /gene="pepA"
                     /locus_tag="VBD11_001261"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6954213.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            8965..9735
                     /locus_tag="VBD11_001262"
     CDS             8965..9735
                     /locus_tag="VBD11_001262"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6954214.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(9756..10421)
                     /locus_tag="VBD11_001263"
     CDS             complement(9756..10421)
                     /locus_tag="VBD11_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6954215.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(10490..10945)
                     /locus_tag="VBD11_001264"
     CDS             complement(10490..10945)
                     /locus_tag="VBD11_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954216.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRFLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
BASE COUNT         3658 a         2173 c         1713 g         3431 t
ORIGIN      
        1 acttaaagtt aggtaaaaca caattctaga ctctagctca taaaccatgt taatccaatg
       61 tattttaata gacatcataa aaaggctgaa taaattcagc cttaaccacg ctattagtta
      121 tagcaaacca atcaaatgac taaagatagg aataaccaaa tcaactacca aaccgataac
      181 caccaccgct atagctgcca tcgcaccttg cagttctccc aattccaatg caaaggctgc
      241 tccgactgta tgaccagagg tccctagacc taatcctgca ccaatcttac tatcattcaa
      301 atggaaaaat gacactaatt ttttacctaa tgcataaata ataactgcat ttaagataca
      361 cgccattgct gtcacagctg tgttccctcc aattgctgct gttattggaa gagcaattgc
      421 tgttgttgct gcttgtggca acattgaagc aattccgact tgactaagcc caaccatttt
      481 agaaatagcg acgataagca taagggaaac aatcataccg attactaaac tgctgagaat
      541 ttctacccaa tattttttaa caacgtcatt tttcttatag agaggaactg caaaagcaat
      601 tgttgctgga tttaaaaacc aaaatattaa atcgccacct ggtttataag cttgtgtata
      661 aaatgtctta acatcggttc ctaaaccttt tgacataagc caaagaatga caataccact
      721 aaccattgca aagaaaagag gctgcaaaag gaagaaacct ttactcttct taaataaatg
      781 ttgtcctatc gtatagagta ttaaagaaaa gcaaatacca aagatgggtg tttttaagag
      841 ttccatatta tttttcctct actttcttac tatagtggtt aggtaatatc tttttaaata
      901 aatctgtgac actgagttct ctgatagcaa gtattaatcg tgcaacgtat gctaccacta
      961 ctaacataac aacagttgag attgtaataa cagcaactag ttgcaagcct tcagctttta
     1021 ggatatcaag gttcgctgct actgatattc ctgaggggac aaacataaaa ccaatcatgc
     1081 ttatcatcaa agccccaaaa gagtcaaccc actctacctt gataatttta gcagttagca
     1141 atacgtacat tagcaccaaa ccaataaccg tcgttgggat tggtagtgat ttcggtaata
     1201 acattgaaat catttgtgaa actaatagaa tagctgcata tattgacatt tgaataaaca
     1261 ttggagcttc ttttgttttg gtttctttac tggtgtgttt catagtcact accccttcct
     1321 tttattacaa tgttttaact tgttttattg atagctatat tgtacttcaa aataaataaa
     1381 acgttttcat tttcagcctc agttaccaaa aaaagaagcc gaattgcaaa ttcaagcttc
     1441 taaacaacaa agggaagttc gcaaaagcac ttttacatgg ttaaatgctc atttaggtct
     1501 tttaaatacg atcgacctac tggaaatttc tctccattag acataattag taacaaagta
     1561 tgattaaacc aaggttgaat ttcctcaatc atctctagat taatcactgt gtttcgatga
     1621 atctggagaa aatatggtgc tacggcgcgt gacttaaacc aattgagtgt tttccgaata
     1681 gtgtaagttt tctgaactgt actaacagtt aattcgccat cttcaacact agctgcaacg
     1741 atatcttgca ttttaacaac aacactgcga tctgataatg tcaaggttaa caattcagca
     1801 ctggcttttt taggtattgc ttgctctaca tctgaagcag tggtgagttg gctcaatttc
     1861 ttcacttttg atagagccat atcaacgcgc tgttgttcaa acggtttcat gatataatca
     1921 acagcattac tttcaaaagc tttcacagcg tagttatcat aagctgtcgc aaaaacaaca
     1981 agtgggggat gtgcaagttg gctcaactga ttagccaaag taaagccatt ttcttcacta
     2041 agcgaaatat ctaaaaaaat taaatcaatt tgctgtctaa ataagatttt ttcggcttca
     2101 ctgatatcct cagcctgaaa aatctcaggg ttgtcaacct cctggctatg ttcaactaaa
     2161 aacgataatt cttgtctggc aaacatttca tcatcaagaa ttaaaatttt catgctcatc
     2221 ctcccttatt ctattaggta ttcgatacca aacttttgta ccattcttgt cgctcgaaaa
     2281 atgaaggcaa cttacactac catataataa attcagcctg ttatttagat taactagagc
     2341 agtacctgtc cccttactct ctgcaactgt ttcttgacct aatttatcaa tgatagtatc
     2401 tgagattcct tgtccattgt cactaacaga aacacaataa taatgaccat ctggctttat
     2461 ttgaaccaat atatggttgt ccgtcttacg ttctttgaaa gcatgtcgaa ctgcattctc
     2521 taccagtacc tgtaaaccaa aaggcggtaa cttcattttt tctggtgcac taatatcata
     2581 agataactga tatttatcag ggaaacgtaa tttttcaaca ttcatataag catccacatg
     2641 tgatttttct tgctcaagcg ttacctcacg atcctgacca ccttgcaaac ttgttctaaa
     2701 aaaagtactt aactgcatca gtgcataacg tgctttatca gaatcaatac ggattaatgc
     2761 actaattgtg ttaatggcat taaagaagaa atgagggttg atttgtgctt gtaaagcctt
     2821 tatctctgcc atactggcta acttattttg ttcctctgtt atccccattg ccagttgtcc
     2881 tgaaaatatt tgcgctaaac caaggactag gttttcctcc acctcagaca ttgtcttatc
     2941 tcctgcaaag tacattttta aggcacacac agttttatca tttattttta gaggaactac
     3001 aatagcagaa tttaactgac agttgtgatc tggacaagaa atcgccgctt tatcttgcgc
     3061 aattcttggt tcgccatcaa aaataacact tttagataag tctgttttga ccggttgtcc
     3121 tgcaatatgg tgatcatggc caacaccaat atgagctaat acgtttgacc gatctgttaa
     3181 tcccacagca tcaaagttag tatgcctctt tataatttcg caaacgctcc tagcagattg
     3241 cggtgtcaaa ccttgtctaa ggtagggcag agtctgtcga gtcaattcaa gaacatctct
     3301 cgtttgaact gcgcgtaact gactttcatt tgacaaataa gttttcaaaa tcgcaaggaa
     3361 aagtgtggaa cctaaactat ttaaaatcat cattggaatg acaatcattt tgacaagttc
     3421 ccatcctgta aaaatgccaa caaatagcat ctggatactt tcggcaataa tactaattaa
     3481 aataacttgg cttgttgaag ggtagagatg gttttcctta agcttatcac caatctttcc
     3541 gctaacaatg ccgactagaa ctgaactgac aatatagaaa gaacctgaaa agcttccttg
     3601 aaaaaagcga tgaactcctc caataaaacc aacaattgat ccaaccagag gtccaccaac
     3661 caaacttgcc gttgtaataa ctaaagtcct tgtattagca agtgagtcag aatgggaaat
     3721 cgttgttaga aaagggcgct cgaccaaact tcgatcccct tttatttcaa ttcctgttat
     3781 attagatata ataacaaaca agccgaaaat gatgacaagg actaccgttt cacgtttaga
     3841 ccgctcttca attaactgtc tgaaataact attatttacc aataaaaagg ctaaaatcat
     3901 aataattcct agcctttgga ataacaacac catcaacata tgcttatcct cctcaagata
     3961 ccattttact ccggaagata aatatagata tcttgataat ctgaaataac cttgtctgcc
     4021 attgataaat cttgcggtgg ataatcagga ttggcaaagc caaagcaata cattcctgct
     4081 gccttagcag ctaaactccc atttcttgta tcttctataa ctatacaaac tttagggtct
     4141 acatctaaaa gctctgcagc tctaagaaaa acatctggtg ctggtttaga agacgataca
     4201 tcttctcctg taaccatgta ttcaaagcat tctgtcacac ccaactcttt tagattacgc
     4261 ttaatatcta ccattggaga tgaggacgca actgctaagc gatagccatg ttggtgtaac
     4321 caatgaatca gttgctgagc tcctttaatg ggcctaacac catcacgcgt cacaatcgct
     4381 tgtcgacgtc tgttcatctc agcaatatac tcttttactg ttttcggtaa gccaaactcc
     4441 tctttcatag cttgccacat gaattcaaaa gtagtcccca tgtactggta ttgatacgag
     4501 acgtccgtat caataccctc ctcacgtaac atttctgtct tattatctaa aaacgtgtac
     4561 tcggaatcaa caataacgcc gtccatatca aaaataatga ctttttccat cttaaacctg
     4621 ctttctatcc cctttattgt aatcgtttgc ctttctattt tcaataaaaa aaggttgctt
     4681 tctgtggaaa gcaaccagta tatttagaaa tgatattttg agagtgattt tatttaaaag
     4741 gggagctcct cttcttccaa tactaaatct gacaaatcac cagaaacatt attttcacgc
     4801 atagcacgtt gggcacggct ttctaacaac tgaaatgatg atgctaatac ttcagtgata
     4861 tagtgcgttt gaccatcctt ttcgtacttg cgcgtacgca attcaccatc tattgaaatt
     4921 aaagagccct ttgtcccata gctcgcaagg gtttccgcta gacgacccca cataaccaca
     4981 ttaataaaat ctgcttcacg ctcaccatta cttcctttaa agcgtctatt aacagcaaca
     5041 gttgcacgcg ttactgactt gtcagttggt gtttttacca tctcaggctt tgctgttaga
     5101 cgcccaatca taataacttt attatacatt gattacctcc tcactattat tattcgaaaa
     5161 taaggaagaa aaatgctaaa atatatgaaa ttgttctggg agaattaaga tgacaaatgt
     5221 aactagttat aaagaaatgc tggctaaacc ttggggaaaa atacaatatg aaataacttt
     5281 tgctcaatta agccatatca aaaatcagaa cgttttagat tttggagctg gtttttgtct
     5341 caccgcacaa catctagcaa aagaaaacaa tgtaactgct attgaaccca atcctaaact
     5401 gctttacgat aatcaaagtg acaatattta taaaatccta ggttcctacg aagcattaag
     5461 agctttgcct gatcaaagtt ttgatactat tatttgtcat aatgttcttg aatatataga
     5521 caaacataat catcctgctt attttgacga attttctcga cttctaaaac ctaatggaga
     5581 actatctctc atcaaacata atataattgg taaaatatta caatcagtta tttttagcaa
     5641 tgatacctcc actgcgatgg aactcttaac aggagaagca aactttaaaa gtgccagttt
     5701 tgaccaggga aatatttata ccttagaaga actaaaacaa aatacaaatt tattagttga
     5761 acgttatcag ggtattcgta ccttctattc attacaacca aaccatttta aaactgaaac
     5821 tgattggcta aacaaaatgc ttgctatcga gctaagtgtt gctgacaaag ctccttataa
     5881 agatattgct tttttgcaac acatcacact taaaaagtca ttgtaacttc ttaagtttac
     5941 aactaaaaat caacttagct gaagctaagt tgatttttac taatttttcc aatgtttatt
     6001 ggcgtcgaaa gactctccaa caattacctg atcagataat tcaatgatac ctctgacttg
     6061 aggagcattt ggaagagcta actcgcgagc actacataac attccaaagc tatcttctcc
     6121 acgaagtttt cctgggaaaa taaggctacc atttggcatc atagctcctg gaagagctgc
     6181 aactgtttta aggcctactg aagcattagg tgccccacaa acgatttgaa ccgtcttccc
     6241 atcgttgatt tctacttgac agatgtgtaa atggtcacta tctggatgat caatcatttc
     6301 tttaatttgc gccacaacaa atgtaggttc aatattatta acaagagagt cttcgaatcc
     6361 ttctttcagt agttctgcat taaggatttt aataacttga tcagataaag ttacttgacc
     6421 tactccctca atagcaatta aacttgacac ttcaaagata ttccaagcaa ctgtttcttt
     6481 actatcttgc agatagacgc gtgctacttg tccgcgacga tcaacatcta gcttagctcc
     6541 ttgactatcc ttaacaataa ccattaaggt atcaccaaca tgctctctat tataagtaaa
     6601 aatcatttat tctcctttaa attttgctat tttttgtctt atttatggtt aatttcagcg
     6661 agaaatttag taatttcagc ctttgttttg cgattcttat taaccaaacg acccagttcc
     6721 tgaccatttt ccacaacaac gaaactcgga atcccaaaaa tattccactg ctgagctaat
     6781 tctatatagt catcacgatt aacacgtaca aagacaaaat ctgagaaatc tttttcaatt
     6841 gatggcatta ctggatatat aaattgacaa tctggacacc aatctgctgt aaaaaagaaa
     6901 acaactttct tagtgctatc aatataagct gctatttctt cataagattc tggtaaaatc
     6961 atttgctcac ctccacataa tcaagaacac cttgacgata aatgaattcg aaaattctgc
     7021 catcttccaa gacaagacca ccactggtca ttttaatatc agattcaaat tcattaaaat
     7081 aaaatacctg aatatcaccc atctgactaa aaagttctct taaggtctga gtgatttgct
     7141 gacgtttata atctgttact tttttagtta gggtataacc agcagctgcc agtaaaccca
     7201 caccagcaag gccagtccca aataagattt ttttattttt cataactgtt attataccat
     7261 accccccgga attagacaca ggaattgatg gaagaaatcc acataacagt tatgaaaaaa
     7321 aacaacttat tccttgtggt ataataggat gacattgtct taagatacca ttatggttaa
     7381 tcattcaaac agaaagaaaa aacatgaaag aaaaattatt agtacctgct ttaaattgtt
     7441 tgctaggtct actcttccta ggtgatcact ggtttttatt tcgtcttcaa aatggagaac
     7501 tttttgctat tgtgacgcta atactgcttt gcacagcttc atttttaatc aataagaatc
     7561 accccaaaaa tttgacttta attgctatgg ttatcacttt tttagccgta ttatctttcc
     7621 tatctacctt tttattagct taagccgata aacattatct ccgttgtatt caacccagtt
     7681 ttcactataa caggctttgt cagtcataac agtcttctgg tagtacttga tattcttttt
     7741 gaaagtcttt atattgactg aaacctgttc tgttctttat ttttaattaa aaggtgctaa
     7801 aatagtattt gtagaattga ggtaattatg tcagatttat ttaacaaaat taaaaccgta
     7861 actgagcttg atgggattgc tggctatgaa cacaatatcc gcaacttcct tcgtcaagaa
     7921 ataactcctt tagttgatca agttgagaca gacggacttg gtggaatttt tggagtaaaa
     7981 aatactcatg agactaatgc tcctaaagtc atggttgctg cccatatgga tgaagtcggc
     8041 tttatggtta gtcatattca gccagatgga acatttcgtg tacttgaggt tggaggatgg
     8101 aatcccctag tagtcagctc acaacgcttt accctctaca cacgttctgg tgatgccatt
     8161 cctgttatat caggctcagt tcctcctcac tttcttcgtg gacaaagcgg tggaacaaca
     8221 ttacccaaaa ttagtgacat tgtttttgat ggaggattca cagataaaaa tgaagctgaa
     8281 agctttggca ttgctcctgg cgatatcatt gttcctaaat ctgaaaccat tttaactgca
     8341 aatcaaaaac atattatgtc aaaagcttgg gataatcgct atggtgtgct tatggtgacc
     8401 gaattgctaa aaagcttaaa agatcaaagt cttagcaaca cacttattgc tggggctaat
     8461 gttcaagaag aagtcggact tcgtggcgca catgtttcaa caactaaatt caacccagat
     8521 atcttcttag ctgtcgattg ttccccagct ggagatattt atggggaaca aggcaaaata
     8581 ggagagggaa ccttaatccg tttttatgat cccggacata tcatgcttaa agatatgaga
     8641 gatttcttgc ttacaacagc tgaagaagca ggtataaaat accaatatta tgctgcaaaa
     8701 ggtggtaccg atgctggggc tgctcaccta aaaaatagtg gtattccttc tacaactatc
     8761 ggtgtctgtg cacgctacat tcattctcat caaacactct acgctatgga tgattttcta
     8821 caagcacaag cttaccttca ggccatcgtt aacaaattag accgctcgac ggtggatatt
     8881 attaaaggtt attaagatag aaaggatggg agtttcataa ggtagacaca ttttagtatc
     8941 tcctttcatc cccatatcaa cattatgaaa attggaatta tcggcgttgg aaaaatggcc
     9001 agtgcaatta ttcaaggcct caaacaaacc caacatgaca ttgtcatatc cggatcttgc
     9061 ttagagcgtt caaaagaaat tgcagaaaga cttgatgtta cttacgccga atctcatcag
     9121 tcattaatca atcaagctga tattatcatg ttaggtatta aacctcaact atttgaaaag
     9181 gttcttcttc cacttgatat cacaaaacca atcatctcaa tggcagcagg aatctcctta
     9241 gcgcgtttaa gccaattaac tcgttctgat ttaccattga ttcgtattat gccaaatatt
     9301 aacgcacaaa tacttcaaag ttgcacagct atttgttaca acaatcatgt atcagatgag
     9361 ttacgacaat tagccaaaga aattacggat agttttggaa gtagctttga tatagcagaa
     9421 atcaattttg ataccttcac tgctctagca ggttctagtc ctgcctatat ctatctattt
     9481 atagaagcct tagctaaagc aggtgtaaaa tatggatttc caaaagaaca agcactctct
     9541 attgtaggac agactgtctt agcttctagt caaaacctat tacaaggaca aaatagcacc
     9601 tctgacttaa tcgacaacat ttgtagccca ggcggaacta ctattgctgg ccttttagac
     9661 ttagaaaaaa atggtctgac gcatagtgtt atttctgcca ttgatgctac tattgagaaa
     9721 gcaaaaaaac tttgagcaaa ttgctcaaag tttttttagt tattcatcat tgtactgatt
     9781 aacacgctat aagcaagagc attgttaata aaatgaatca ttatactata ttctaagtgc
     9841 tcagttttat aataaacaaa agataaaata gcacccatcc cagcataaat aataaaacta
     9901 cctatatcag aaggggaatg aataagagca aaagctaaag taccgatgac aaaaccccat
     9961 ttttgatgtt tcgcaaaaag ttctttaggg ataatatagc gaaatataat ttcttccata
    10021 attggtgccc ctattatggc aaaaattaac atcaaagcta gtggaagttg ctttgttaat
    10081 tccataatta ctgtttgatt tgctgtatct ttaacccctt gcatagtcat aatgaggtaa
    10141 ccaataattt gtggaaccct caatgataac caacctaaga gaatagtaac taatgctttc
    10201 cttgtcaata atactttcca tctgattatt tgtttaacct tggcataata aaggaaaaga
    10261 gcaataataa gtaattctaa gccaattaac aatatactag taaaaggcgt ttgatagtga
    10321 ttctttagga gccacacagc caatatagga acaagttgat taataactaa aaatgctatt
    10381 agcaaagcta aaattttgcc ttttttaata ataaatgaca caaaaacctc caaaataaat
    10441 tattaacttt tttctatcat aacatatttt caaatcttat agatttatct cacaataact
    10501 tctgtaaaat aaagtaaatt aataaataac cgtataaaac aaaagagagt atcaatagca
    10561 agaactttgc ataggaaaaa gttataccaa accagacatt aatcagacac aataccacta
    10621 aaacaaggac agataaaagg aaaaggagtc gacaagacat attagcaata aattgattac
    10681 gctcatcaaa aactttggta tagtaatgtt gaaatcgctt ttcactaaat gttatcacca
    10741 aaaaataaaa acttcctgca aataaggcaa tacctgaacc aaaggaaaaa cctttagtaa
    10801 aattagattc aaaggtatgt agaaatgatg atactatcat aatcaaacta catacactaa
    10861 aaacaaaaga caaaaatctc atatatactt tgtaatcctt cggagatact ttactattgg
    10921 gtgacaataa tccttttatt aacatagaat actactttct aaaacttcaa tttaa
//
LOCUS       DATROY010000064        10717 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.065, whole genome shotgun sequence.
ACCESSION   DATROY010000064 DATROY010000000
VERSION     DATROY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            294..1463
                     /locus_tag="VBD11_001265"
     CDS             294..1463
                     /locus_tag="VBD11_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6954217.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1460..1606
                     /gene="rpmG"
                     /locus_tag="VBD11_001266"
     CDS             1460..1606
                     /gene="rpmG"
                     /locus_tag="VBD11_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6954218.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1653..1889
                     /gene="secG"
                     /locus_tag="VBD11_001267"
     CDS             1653..1889
                     /gene="secG"
                     /locus_tag="VBD11_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6954219.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2002..4407
                     /gene="rnr"
                     /locus_tag="VBD11_001268"
     CDS             2002..4407
                     /gene="rnr"
                     /locus_tag="VBD11_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6954220.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4410..4877
                     /gene="smpB"
                     /locus_tag="VBD11_001269"
     CDS             4410..4877
                     /gene="smpB"
                     /locus_tag="VBD11_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6954221.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4961..6349)
                     /locus_tag="VBD11_001270"
     CDS             complement(4961..6349)
                     /locus_tag="VBD11_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6954222.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6478..7404)
                     /locus_tag="VBD11_001271"
     CDS             complement(6478..7404)
                     /locus_tag="VBD11_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6954223.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7533..7925)
                     /gene="gloA"
                     /locus_tag="VBD11_001272"
     CDS             complement(7533..7925)
                     /gene="gloA"
                     /locus_tag="VBD11_001272"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6954224.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8013..8687)
                     /locus_tag="VBD11_001273"
     CDS             complement(8013..8687)
                     /locus_tag="VBD11_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6954225.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8954..9796
                     /locus_tag="VBD11_001274"
     CDS             8954..9796
                     /locus_tag="VBD11_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6954226.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            9987..10709
                     /locus_tag="VBD11_001275"
     CDS             9987..10709
                     /locus_tag="VBD11_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6954227.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT         3660 a         1702 c         2076 g         3279 t
ORIGIN      
        1 tattattagt aagcctagga attatagttg ttattttaat tattggctat ttagcagttg
       61 tagtatttgc tccaaaaact ttccgtttaa aaagttaatt ttaattgtgg tataataaca
      121 gaggcatgtg ataacatagg tttagttaca taaaggaaat tataatggaa ataacatctc
      181 aaatagatgt taaatctcaa taaaactgct aagatagtta gtggtagaaa agtttagaaa
      241 gaagatgatg ttttatctca gttgaaacgg ttaactggaa gcaaaattta aaagtggctt
      301 ggctcggtaa tttttttact ggggcaagct tttcacttgt tatgcctttt atggtattat
      361 atgtagaaca attaggagca cctagcaaca aagtagagtg gtatgcaggc ctatcagtat
      421 ccttatcagc cttgtcttca gctttagttg ccccattatg ggggcgctta gctgataaat
      481 atggtagaaa accgatgatg gtaagagcag ggttgatgat gacttttact atgggaggcc
      541 ttgctttcat tcatagtgtt acaggacttc tgattttacg tatattaaac ggcatttttg
      601 caggctatgt acctaactct acagctttga tagcaagtca agctcctcaa gaggaatcag
      661 gatatgcctt aggaacctta gcgacaggag tgactggagg tatgttaatt ggtccacttt
      721 tagggggact tttagcagag tggtttggta ttagggaagt ttttttatta gtgggaacca
      781 tattgttaat atctacttta atgaccattt ttatggtaaa ggaagatttc aaacccattt
      841 ctaacgaaga aaccatgcca actacagaag tgttcaaaag cgttaaaagc ttacaaattt
      901 taatagggct gtttgtaaca agcatgatta ttcaaatttc tgcacaatca attgcaccta
      961 ttttaacact ttacattcgt catttaggac agactgaaaa cttgatgttt gtttccggat
     1021 taattgtttc tggtatggga ttctctagta ttctgagtag tccaaagcta ggaagaatcg
     1081 gggatcgcat aggcaatcat cgtttgctat tattagctct actgtatagt ttcctcatgt
     1141 atgtcttatg ttcattggca cagacttcat tacagctagg ggttattaga ttcctgtatg
     1201 gttttggaac aggtgccttg atgccgagta ttaattcaat cttgactaaa attgctccaa
     1261 gacaaggttt atcaaggatt ttcagttata accaaatgtt tagcaattta ggacaagtat
     1321 taggtccttt tgtcggttca gcagtttcta ttcatttagg attccgatgg gttttctttg
     1381 tgactagctt catcgtttta gctaattttg tttggtgttt tattaatttt agaaaatata
     1441 ttagagttaa ggaaattgtg tgagagttaa aattaattta aagtgtagct cttgtggtag
     1501 taagaattat ttaaccagta agaataaaag taatcatccc gataaaatcg aggttccaaa
     1561 gtattgccca aaggagagaa aagtaacact acatattgaa tcttaataag ttttatgtta
     1621 gaatgtactt tgaccatagt ggaggaatta acatgtataa cctattactc accattctac
     1681 ttgttttatc agtattattg attatctcaa ttttcatgca accacaaaag aatccaagta
     1741 gtaatgtttt tgatagtagc ggttctgaag ccctttttga acgtagtaaa gctcgaggat
     1801 ttgaagcttt tatgcaacgt tttacaggtg ttttagtctt tttctggcta ctaatcggct
     1861 tagtattatc tattttatca agtcattaat gatagctaaa tacagataag tgaggatggg
     1921 ggatagccca acctcacttt ttgttagtag aatggttgta gagtaataat aaagtgacta
     1981 aagttactag aaaaggaaat aatgaaagaa aaaattataa aatatttgga agaacacggt
     2041 aaaaccagcg ttaatgattt agctgctgcc ctagatatgg caggcgcaaa agcatttcca
     2101 aaattaatca aaacaatctc aaatttggag agccacaggc aattaagatt tgacgataat
     2161 ggaagtcttt ctcttcaaaa gaaagaagct aagaaaaagg aaatcacagt aaggggtctt
     2221 tttagagcaa ataaagcagg atttggtttt cttagtattg accaagacga agatgatatg
     2281 tttatcggaa aaaacgatat tgcttatgct attgatggtg acacggtaga agctgttgta
     2341 aagaaaccag cggaccgtct taacgggact gcagcagaag cgcgtgttgt taatattgtt
     2401 gaacgtagtt taaagacgct tgttggtaag tttgtcttag acgatgagcg ccctaaatac
     2461 gcaggatata tcaaatcgaa aaatcaaaaa atcaatcaaa agatttatat acgcaaagaa
     2521 ccggtcgttt tagacggaac agaaattatc aaagtagata ttgataaata tccgacacga
     2581 ggacatgatt acttcgtagc aagtgttcgt gatatcgttg gacatcaagg agatgttggt
     2641 attgatgttt tagaagtctt ggaaagtatg gatatcgtct cagaatttcc tgaggacgtt
     2701 attgcagaag ccaatgctat cccagatgct ccaacggaga aagatttaat tgggcgtgtt
     2761 gatttacgtc aagaggtcac tttcactatt gatggagcgg acgccaaaga tttagacgat
     2821 gctgtgcata ttaaattact ggataatggt cattttgaac ttggagttca tatcgcagat
     2881 gtatcatact acgttactga aggctctgcc ttaaatcgtg aagcacttag tcgtggtacg
     2941 tccgtatatg tcacagatcg cgttgtacca atgttaccag agcgtctatc taacggtata
     3001 tgttcattaa atccaaatct tgatcgttta acgcagtctt gtattatgga aattgaccaa
     3061 aatgggagag ttgttaatca tcaaattaca cagtcagtga ttaataccac ttatcgtatg
     3121 acttatatcg ctgtaaatga tattattgct ggcgatgagg aaatctgctc agaatatgag
     3181 tctattgtgt cctcagtaca gcatatggtc actttacacc atactttgga agctatgcgt
     3241 acgcgtcgtg gagcgctgaa ttttgacaca tctgaagcaa aaattatggt taatgataag
     3301 ggaatgccag ttgatattgt tattcgcaac agaggcattg ctgaacgcat gattgagtca
     3361 tttatgttgg cggcaaatga aacggttgct gaacattatg ctcgtctaaa actgccattt
     3421 atttatcgta tacacgaaga gcctaaagct gaaaaattac aaaaattcat cgattatgct
     3481 tctgttttcg gtgttcagat tcaaggaaca gcaactaaaa tcacccaatc tgctttacag
     3541 gactttatga aaaaagtaca agggcagcca ggaagtgaag tgttatctat gatgttattg
     3601 cgctcgatgc aacaagctag atattctgaa cacaaccatg gacattatgg acttgctgcg
     3661 gaatattaca ctcactttac tagccccatt cgacgttatc cagacttgtt agttcatcgt
     3721 atgattcgtg attatgatga taaagcgatg gataaagctg atcattttgc taaccttatc
     3781 ccagaaattg caactcaaac gtctagtctt gagagacgtg ccattgatgc tgaacgtatt
     3841 gtagaggcaa tgaagaaagc cgaatatatg gaagaatatg ttggtgagga atttgaaggt
     3901 gtggttgcaa gcgttgttaa atttggtatg tttgtagaat taccaaatac tattgaagga
     3961 ttgattcacg taacgacctt gcctgaatat tatcacttta atgaacgtac gctgacgctt
     4021 caaggtgaga aatctgggaa agttttccgt gttggtcaac aaattaaggt taagttgatt
     4081 cgttctgata aggaaactgg tgatatcgat tttgattatt taccatcaga ttttgatatt
     4141 gttgaaaagg taagtaaaag ctcgcgtgag ggtcgtccta atagaagttc taaacgagaa
     4201 catcagcata gaatttctga tcgtgataat aagaataaaa atacatctaa gaaaaaagca
     4261 tctcgaaaac ccaaaaggaa ctcggatagc aaatcacatc atcataaaga cgatagaaca
     4321 acaggttcaa ccaagaaaaa aacgaaaaaa cctttctata aaggcgttgc taagaaaggt
     4381 cagaaacgga aatctaagaa aggttaagta tggttaaagg tcaaggaaac gttgtagcac
     4441 aaaataaaaa agcgcatcac gactatacta tcgttgaaac aatagaagca ggaattgttt
     4501 tgacaggaac agaaatcaaa agtgtccgcg cagcgcgtat tacattgaaa gatggatatg
     4561 cacaaataaa aaatggtgaa gcgtggttga ttaatgttca tattacacca tatgatcaag
     4621 gtaacatatg gaatcaagat cctgatcgta cacgaaagtt attactaaaa aaacgtgaga
     4681 ttgaaaaaat atcaaatgaa ctgaaaggga ctggtatgac tctggtacct ctaaaagttt
     4741 acttaaaaga tggttttgct aaagttctat tgggtttggc aaaaggaaaa catgattatg
     4801 ataagcgaga gtctatcaaa cgtcgtgagc aaaaccgtga tattgcccgt cagttaaaaa
     4861 actataattc acgatgatta tcaaaaggtt agggaattgc ctaacctttt ttggacgaaa
     4921 aaagtgaagt tgattgacca acttcactaa taatatagac ttaatgtgat ttatgtttga
     4981 agtgactata taaaccaaat ccaataatcc agatggttgc tccaatagcc ccgatgtatg
     5041 ttgaatcttg caagaagaga gaaacgaata caaagaggaa gaagagaagt gtgattggtg
     5101 aaaggatttt gtaggcaggc attttataac catcttccat aaaatcaggt gatttacgat
     5161 atttccaatg tgcaatcata ataagaacat agatagaaat gtaaacacca gaagatgatg
     5221 cagtaattaa ggagaaggca tctgaaatac caggtaaaac actgataagt gctgaagctc
     5281 caacgattac agctgatgca ataatagctc gacttggaac gctttgacga gataattggt
     5341 ctaattttaa cgcttttgta agagcatttg gtgattcatt agccaattgg aataaatgac
     5401 gccctgttga gtacaaagta gaattaagag cagatgctgc agacgttagg acaacaaagt
     5461 taatcaaagc ggcagcccac ttaatcccag ctagtttgaa tactgtaacg aaaggtgact
     5521 cattaactgg taactgttgc catgggaaga tggccataat tgctaataat gaacctgcgt
     5581 agaataaaat aatacgcatc ggaatttctt ggattgcttt tggtaaaact ttacgtggat
     5641 tagctgtttc agaagttgtt actccgacaa attcaatagc taaataagca aagaatacca
     5701 tttggaaagc catgaagaaa ttcaatttac ctttagggaa ccattcaaaa tgattggtga
     5761 tatttgagat tgaagcatgg tatccgtgac cggtgtcaaa gtttgtcaat accataaaga
     5821 taccggttgc tattaaacca agaattgcaa taacttttat catagcaaac caaaactcgg
     5881 tttctccgaa tgcttttaca gcaattaagt tgatagaact taggatagct aaaaaaacaa
     5941 tttggataat ccaggatggc cattcaggga accaaaattg aacatactct cctacggctg
     6001 taatctctgc cataccgata aatattaggg aaatccaata agaaagtgtt gagaaaaatc
     6061 cccaaccggg tcctaaatat ttggtaatga aattaataaa agtatgctga tttggatctt
     6121 gataaagcat ctctccaata gcacgcatca tgaagaacat gaaagcgcca gtgatagcat
     6181 agacaagaac tattgaagga cccgtcaggc taattgaacg acctgcacca agaaaaagcc
     6241 cagttccgat tgtccctgca atcgcaatta actgtacgtg acgattttct aggccgcgta
     6301 ccataccatt ctcagatttt tgagtgtggt cattattatt ttttgacatt ataaatactc
     6361 catctatttt tgaaaaactc cttcattata taattttttt gcaaaattcg caataattac
     6421 tatacaatat ttttctatga taaaatctcc tagctaagtg agctaagaga tttcaattca
     6481 tccctcttcc ttgacaatat agactgggcg ttttttagtt tctaggaaaa tcttagagat
     6541 gtatttgcct ataattccta gcgacaataa ctgaatacca cccataaaaa gtacaatggt
     6601 aactgtgctt gcccaacctg aaacgggatc cccaaatagt aattttcgaa ttataataaa
     6661 aatgatagct aaaagtgata gtactgaact aagggttcca atccaggtag ctatagctaa
     6721 tggaatttct gagaagttga tgaaaccgtc tagagagtat tttaagagat taaagaaact
     6781 ccatgaggtc ttacccgcaa ttcgttctcg attctcataa ctaatataag cgacatcata
     6841 tccaatccag gaaaaaatcc cttttgagaa gcgattcact tcatttagct cgagaatact
     6901 gtcaacaact tgttttgtca tgagacgaaa atcacgcgca ccatcaacca ttttagtatc
     6961 tgacacttga ttgattaatt tataaaaaag cttggcaaaa agagagcgta tcaacggctc
     7021 ccctttacga tcttttcgcc ttgtagcgac aatatcataa ccttcttgaa gttttaggta
     7081 catttctggt aataaagtgg gtggatcttg caagtcaaca tccataacag tgataaaatc
     7141 accagtagta tgttctaaac cagccaataa agcagcatct ttaccaaaat ggcgtgaaaa
     7201 tgaaaggtaa tgaacattgt caaattgttt agctgctttc ttaagaagtt cgagtgtacg
     7261 atcggtagaa ccatcgttta cgaaaatata ttcaaaatgg gtgtaacgag ccatttcgga
     7321 ttcaactttt ttgatttctg ttaaaaaagt tgagacagtc tcttgttcat tataacaagg
     7381 gacaataact gataataaag ccatttgtaa ttctccgtat cattcttgct taaattttaa
     7441 cataatatgg gaaaaaatag aaagattata aggtaatcat aaaaaggact aggtttttgc
     7501 ctcagtcctt aacttaatat gatgagatgt tattagtcag ctttaaattg actgagtcga
     7561 ataacttcaa ttttatagcc atctggatct tgaataaagt agaacatatt aggtttacct
     7621 ggtaatccag aaatttttgt tacagagtaa cctgctttct gatgggcatc ataagtagtt
     7681 tccaaatcgt ctacaccaac agcaatgtga ccatacccat ttcctaaatc ataagcttcg
     7741 tgatcatagt tataggttaa ctctaattcg taactagggt cgtcctctag ggacatatag
     7801 actaaagtaa attgattttc aggaaagtca ttacgacgaa cttccttaaa accaagtgct
     7861 tcttgataaa aagcaattga ggcgtccaaa tctttaacgc gaatacaagt atgtaaaaat
     7921 ggcatagtct ctctcctatt tctttattaa acgtattatg tcaaaataaa attatctttt
     7981 caaaaatatt gattataaat taggctgtct atttactcaa cccaagtaat gacctcttga
     8041 cgttctttcc tagcacggga atgtttaggt tcacgcaaac gataaccaaa tgaaaccata
     8101 caagaaatag cttctttctt atcgtctatt aacccttctt tactaagaat attattgacc
     8161 ttttcatagt caaatccttc aattgggcat gaatctacgc cgatcatagc agcagccgtc
     8221 atcatattac caagtgcgat ataagtttgt tttgctgtcc aatcccaaag agaacgttca
     8281 gaatcaattt tcatatcgtt ttcttggaag gatttgtaga gaggtatcct actctcaagg
     8341 gcggctggat cacctagacc acgtcgaatg agactgttaa tcatgctatc agcgttataa
     8401 taagcacctt tttcagctag tagtaaaaca aagtgactag cagtatctag ttgatattgt
     8461 gctccccaag caacttcctt aagtttatct cgaaattttg caattgtttg tctatctagt
     8521 actaaaaatc gccagccttc taaaccgact gaagaaggac tcaaccaagc agtgtctaaa
     8581 atatactcca tatcttcttt aggaatatca ttattattat aaacacgaat cgccatacga
     8641 aaatcaaaag ctcttcgaac ttgttttttg atatcttctt ttgtcatttt agatcctcct
     8701 gttattaatg ctatcatatc acaatttaga aaaaatctca gcaggcggta tcgtggcaaa
     8761 gtagagctac aaaattctaa tgactgtcag aaaaataaag ggaatgaaca aaatgatagg
     8821 gttttgatga agaaagttat ataattttac ataaggaggg gataatacca tatatcaaac
     8881 tatcatttag atgtttaaaa tagatgtttt tcactatgaa attcattttt gtcacataga
     8941 aaaggagatt gttatggaaa catatactct atcgaatact cttaacattc ctaaaattgg
     9001 ttttgggaca tggcaactaa ctgaaggtga agaagcttat aaagcagtga ctcatgcttt
     9061 aaaagttggt tatactcata ttgatactgc tcagatttat ggtaatgaac atagtgttgg
     9121 acgtgctata agagacagtg gtttggcgcg agaaagtatc tttttgacaa ctaagatttg
     9181 gaatgacaaa catgactatc atttagccaa agcttctatt gatgaatctc tgcagaaatt
     9241 aggtgttgat tatattgatc ttcttttgat tcattggcca aatccaaaag ctcttcgtga
     9301 aaatgatgcg tggaaagctg ggaatgcagg cacttggaaa gcgatggaag aagcttataa
     9361 agaaggaaag gtaaaagcta ttggtgtttc gaatttcatg aaacatcact tagaggcttt
     9421 atttgaaacc gccgaaataa aacctatggt taatcaaatt atcctagcac caggttgtgc
     9481 tcaagaagat ttggttcgct tctgtaaagg aaatgatatc ttattagaag cttacagtcc
     9541 atttggaaca ggtactattt ttgaaaatga atctataaaa gctattgctg aaaaatatgg
     9601 taaatcagtt gcacaagtag ctcttcgctg gtcacttgat aatggctttt taccattacc
     9661 taagtcagcg actcccaaaa atattgaagc aaaccttgat atttttgatt ttcaattaaa
     9721 cgaagatgat attgctacct taatacaact agattcaggt attaaaccca aagatccaga
     9781 taatgtctca ttttaataag aaaagatgga aatgtatttc catctttttt atgtgggata
     9841 gttccttaaa gttaagaaaa acattaagac ctccattgac gaggagaata tattaatgat
     9901 agtattacta gaaaagtagt aataaaggat gagtatggta aaatccttta caatatatca
     9961 ttatcgaaag aggttataac atctaaatga gacttgataa atttttagta gaatgcggac
    10021 ttggttcacg tactcaagtc aaattaatat tgaaaaaaaa gcagattagt gttaatagta
    10081 atagcgagac atctccaaaa gtacaggtag atgaatatag agatgagatt aaatataatg
    10141 gaactctagt gtcatatgaa aagtttgttt attatatgct tcataaaccc aaaggtgtta
    10201 tatcagcaac ggatgatcct tcgcataaaa ccgtattaga tttgctagac aaaactgctc
    10261 gagataaagc tgtttttcca gtggggcgtt tagatattga tacaacaggc cttttgttaa
    10321 taactaataa tggggaatta gcgcataaaa tgttatcacc taaaaaacac gttgataaat
    10381 gctatgaggc aaaaatctca gggataatga cagaagacga tattttggca tttgataaag
    10441 ggattattct gaaagatttt acttgtttac cagcattatt agaaattgtg gaagtaaatc
    10501 aggtgaagaa gcagagtctt gttaaaatca ctattaagga aggaaaattc catcaagtta
    10561 aacgtatggt tgctgcgtgt ggcaaggaag tcttagagct aaagcgtcta agtatgggaa
    10621 atctacaatt agataaacag ctagaaagtg gacaatggag acggctaaca ataaaagaaa
    10681 tagaaaaatt agaaaaacat atgcaataat ataaaat
//
LOCUS       DATROY010000065        10683 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.066, whole genome shotgun sequence.
ACCESSION   DATROY010000065 DATROY010000000
VERSION     DATROY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..152
                     /locus_tag="VBD11_001276"
     CDS             <1..152
                     /locus_tag="VBD11_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954228.1"
                     /translation="LSFYDYSIVKSYVADGDFLIYKGICKTGMVAYLLDNRKKWKSVE
                     VWDID"
     gene            137..598
                     /locus_tag="VBD11_001277"
     CDS             137..598
                     /locus_tag="VBD11_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954229.1"
                     /translation="MGYRLNRYVCVRKDVFLALVMLLVTLIVSLVFLLKSDGISFFSD
                     QYYRSQLTAKEAKNVINKNYNIVFYKKSCPYCKIAKKEILEKSKGSDITTFFADVETV
                     SGKELVRKYRISKAPSIVAVRGDNLKVNLYAKDDEDGRMIVEKKVIKDSFR"
     gene            618..881
                     /locus_tag="VBD11_001278"
     CDS             618..881
                     /locus_tag="VBD11_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954230.1"
                     /translation="MGVKSNEELKREQYLAEFSRVVTNFKWNASRIYFGDIIAIKLCF
                     KEALKQIDILHERAILRRDEVSTFYYWAKRNINNVYQELIEKK"
     gene            897..3158
                     /locus_tag="VBD11_001279"
     CDS             897..3158
                     /locus_tag="VBD11_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6954231.1"
                     /translation="MKQTKVLSGIAIATLSTVSVAVNADEVKSLSLETTANQTNHITE
                     VTQEQVDSAKAKSDRATSDVETQQNVVNTAQNQQNQAQQTLVNATKKLNATQKLAQES
                     TQENKVKAQQTIDIAKQMLSQIGAKQKTAETEQVKAQEAVKAQQIVVAHDKQDVATKT
                     TDVKQAQSSVDRAKDALTNTTVNSDLNKAQSNITTKTADVKTATDALTKAQATDKTLT
                     NQKAKAQQNLDNAKQNLSVKDAQLAQANATVNQNKFKTALGQSHYYNQRDGAWASVYG
                     GHTFASTGCVPSALAMVYSDLSRKTVTPKEVADYLFNQTNEFNKNFGGTSGRGIVEAT
                     KAFGYVVTHLASQNAIKEALKAGHHVVAAVQNNKFSPWGPQYSHEIVLRGSSNGNTYV
                     YDPYNRDNNGFYSVDRIWNEQSRDSIDTAGVGVPFFSIMTKDMANALTKQSQALASQQ
                     VAQRQLNDAQAKAIGLNAVTMQTPIAQANLIKAQSNLKDAQDALTKAQEAVKLANQDN
                     VKKQADLIKAESKLTDAQKQLADAQVKLTTNTTKLNQLKQVLAEASQQVAQANQDYKQ
                     AKDNLTQKTAYLTNLRNAQANLLKVQSDVAQAKDNLANKIAKLQREVAYLQELKTKAV
                     DAQSQYQKVLSAYKSVLEAKEKLKLAEEKAQIEKVRQEAVAVVDETGKITSYITSKPK
                     LQEAVKAQQIVVANDKKDVVTKTVDVEQVSVVKSSVLPSTGDAKTGLIAVLGMLLSLS
                     GFLGVRKNNKNNI"
     gene            3247..5838
                     /locus_tag="VBD11_001280"
     CDS             3247..5838
                     /locus_tag="VBD11_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6954232.1"
                     /translation="MYKNQNTKGHGFFRKSKAYGLVCGIALAGAFTLATSQVSADQVT
                     TQATTQTVTQNQAETVTSTQLDKAVATAKKAAVAVTTTAAVNHATTTDAQADLANQTQ
                     TVKDVTAKAQANTQAIKDATAENAKIDAENKAESQRVSQLNAQTKAKIDAENKDAQAK
                     ADATNAQLQKDYQAKLAKIKSVEAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALY
                     NQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMTAKTKPDGSFEFNHDMIDG
                     VKTIGYGKLTGKVNHHYVANKDGSVTAFVDSITLYKYEYRNVAQNAAVNQNIAFRVLT
                     KDGQIIFEKKHNGNKTFAETLNKTLQLNLKYELKPHGSSGSVEVFKIHDDWVHDTHGS
                     ALVSYVNNNDVVPNVVIPKQPTPPSTEKVTPEAEKPVPEKPVEPKFVTPTLKTYTPAQ
                     PKVKPHVSIPEKINYSVSVHPVLVPAANPSKAVIDEAGQSVNGKTVLPNAELNYVAKQ
                     DFSQYKGMTASQGKIAKNFVFIDDYKDDALDGKSMKVNSIKASDGTDVSQLLEMRHVL
                     STDTLDEKLQTLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDVTYNLSFKVKKEF
                     TKGQIQNGVAQIDFGNGYTGNIVVNDLTTPEIHKDVLDKEDGKSINNGTVKLGDEVTY
                     KLEGWVVPTGRSYDLFEYKFVDQLQRTHDLYLRDKVVAKVDVTLKDGTVIKKGTNLGE
                     YTETVYNKKTGLYELVFKKDFLEKVARSSEFGADDFVVVKRIKAGDVYNTADFFINGN
                     KVKTETVVTHTPEKPKPVEPQKATPKAPAKGLPQTGEASVAPLTALGAIILSAIGLAG
                     FKKRKEN"
     gene            5898..6194
                     /locus_tag="VBD11_001281"
     CDS             5898..6194
                     /locus_tag="VBD11_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954233.1"
                     /translation="MKQETLVLLPNFIGDAIYEGFEAGPAQLNGKKGSYTRHILFNEK
                     HGKFYVYTPASSRVIGNGKMVTVEKPLLFIDNTINGRNIEPAKNVWAEKLIEVK"
     gene            6216..6677
                     /locus_tag="VBD11_001282"
     CDS             6216..6677
                     /locus_tag="VBD11_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954234.1"
                     /translation="MAKIGLNFGEKLQIADKEYRTFNDGLDVNVLLGDLTWRRYEEPV
                     QFTEDDTTQPPDRFGRYPQKPTGEVKWNDIAVFSTAQNETIFVSITDMPSYAIEDLGL
                     KLGDPIELSDVVVTWSNVSGGMFKIFASSLKKKDVANNGNASKNEASKVNK"
     gene            6697..8415
                     /locus_tag="VBD11_001283"
     CDS             6697..8415
                     /locus_tag="VBD11_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6954235.1"
                     /translation="MARLIPQYRGIKVKPYMRYMSYYLFSFLFILFLTPVGVYSYYYL
                     DLLKMMDKMSMISVGTGLFLAFFVSWYLTWFLQEANPLFNKLDRLKRMSKFLYENGYV
                     YEKRKKSNKKTKTKYRFPKVYVKQGKYDLSVSFEMAGGKFQKKFKDIGGELEDTFFMD
                     FMEKTDDPRFKIYKLAYSAFLSRITVKDVIWDKDKGIKLMDGYYWDFINDPHLLVAGG
                     TGGGKTVLLRSILRCLAEIGVCDICDPKRADFVTMSDLSAFEGRIAFEKADIIEKFEN
                     AVTIMFARYDFVRNEMKRLGHKDMKKFYDYGLEPYFFVCDEYNALMSSLSYQEREIVD
                     NAFTQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYVMMFGDE
                     NRNKEFRFIKYLAGRRVYGRGYSAVFGEVAREFYSPLLPKNFSFYDAFEKINRHENPF
                     DPTENQEVSKAILKDESLREFVEKTSENELLKGSVGFDFDDEMEEMENMSIGDLAKKT
                     GFSFGKIKNLIDMIEKDNYMTFSREDEKVVLNDSDAVVLESLLTQKDEFEGSWKELLL
                     NYFKGE"
     gene            8703..9935
                     /locus_tag="VBD11_001284"
     CDS             8703..9935
                     /locus_tag="VBD11_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6954236.1"
                     /translation="MDAIYLKKFRKKTGLKQKEFALSSGLTLKLLRNYEQGKRKLTLE
                     KYQEIKSHFGYLVENDSSRLQVMIDYVRITLKDVRDLEFFCRNFLHCAFKEFQPFESK
                     LMNYNHLWKRGDIWIFDFADKHETGNFQITVQLSGRGCRQLELLMETEKFTWHDWLSY
                     LRNSYRDDMNVTRFDIAIDELYLGKDRENEQFHLSDMISKYYRHELDFESLRTWNYIG
                     GGSLNFSDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYEIAKQEGITVEEALEIFEL
                     WNRYEIRLSQSKANAAVDEFISGVPIGEISRGLIVSKIDVYDGKNEYGSFQADRKWQL
                     MFGGVEPLKFVTKPEAYSIERTLRWLSDSVSPSLAMIREYDMIVDGDYLQTILNSGEV
                     NERGEKILDSIKASLGIL"
     gene            9951..10328
                     /locus_tag="VBD11_001285"
     CDS             9951..10328
                     /locus_tag="VBD11_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954237.1"
                     /translation="MYSNREFAYFVNRRNNLDKMIDLLVFMIPDREFYYPEIQTGELR
                     DYQIDIYDLIKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLEKQKEIVIRL
                     AGKYRLEKRILMRREMFRDEEVD"
     gene            10312..10612
                     /locus_tag="VBD11_001286"
                     /pseudo
     CDS             10312..10612
                     /locus_tag="VBD11_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737133.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3917 a         1409 c         2127 g         3230 t
ORIGIN      
        1 aattatcttt ttacgattat tctattgtta aatcgtatgt ggctgacggt gattttttaa
       61 tttataaggg tatttgtaaa acaggtatgg ttgcttacct tttagataat agaaaaaaat
      121 ggaagtcggt tgaagtatgg gatatagatt gaataggtat gtttgtgtta ggaaggatgt
      181 ctttttagct ttagtgatgc tactagtgac gttaattgta tcacttgttt ttttattaaa
      241 gagtgatggc atctcatttt ttagtgatca gtactatagg tctcaattga ctgctaaaga
      301 agctaaaaat gtgattaata aaaactataa tattgttttt tataaaaaga gttgccctta
      361 ctgcaaaata gcgaaaaaag aaattttaga gaaaagtaaa ggtagtgata taactacttt
      421 ttttgctgac gtggagactg ttagtggcaa ggagctagta cgtaaatata gaatatccaa
      481 agcgccaagc atagttgctg taagaggaga caacttgaaa gttaatttgt atgcaaaaga
      541 tgatgaggat ggtcgtatga ttgtagaaaa gaaagtaata aaagatagtt ttagatagtt
      601 atagaaaggt atttatcatg ggggtaaaaa gtaatgagga attgaaaaga gagcagtatt
      661 tagcagagtt tagtagggtg gttactaatt ttaagtggaa tgctagtaga atatatttcg
      721 gagatataat tgcaataaaa ctttgtttta aagaagcttt aaaacaaata gatatattac
      781 atgaaagagc tattttacgt agagatgaag ttagtacatt ttactactgg gctaaaagaa
      841 atataaataa tgtttatcaa gaattgattg aaaagaagta aattgaggag atttttatga
      901 aacaaactaa agttttatca ggtattgcaa ttgcaacatt aagtacagta agcgtagcag
      961 taaatgctga cgaagttaaa tcattatcgt tagaaacaac tgcgaatcaa actaatcaca
     1021 ttacagaagt aacacaagaa caagttgaca gtgcaaaagc aaagtctgat agagcaacga
     1081 gtgatgttga aacacaacaa aatgtagtta atactgcaca aaatcaacaa aatcaagctc
     1141 aacaaactct agtcaatgca actaaaaagt tgaatgcaac acaaaaacta gctcaagagt
     1201 caactcaaga aaataaagtt aaagcacaac aaacaattga tatagctaaa caaatgttgt
     1261 cacagattgg agctaaacaa aaaactgctg aaactgaaca agtcaaagcg caagaagctg
     1321 ttaaagcaca acaaatagtt gttgcacacg ataagcaaga cgttgcaact aagacaactg
     1381 atgttaaaca agctcaatca tcagtggata gagctaaaga tgctctgact aatactacag
     1441 ttaatagtga tttaaataaa gcacagtcaa acatcacaac taagacagct gatgttaaaa
     1501 ctgcgacaga tgcacttaca aaagcacaag cgactgataa aacacttact aatcaaaaag
     1561 caaaagcaca acaaaatctt gataacgcaa aacaaaattt atctgttaaa gatgcacagt
     1621 tagcacaagc taatgctact gttaaccaga ataagtttaa aactgctcta gggcaaagtc
     1681 attattacaa tcaacgtgac ggcgcatggg ctagcgtata tggagggcat acttttgctt
     1741 caactggatg tgtaccttcg gctttagcga tggtttattc tgatttatca cgcaagacag
     1801 ttacaccaaa ggaagttgcg gattatcttt ttaaccaaac aaatgaattt aataagaatt
     1861 ttggtggaac aagtggaaga ggtattgttg aagctactaa agcgtttggc tatgttgtga
     1921 ctcatttagc aagtcaaaat gctataaaag aagctttgaa agcaggtcat catgttgttg
     1981 ctgctgtaca aaataataaa ttcagtcctt ggggacctca atacagtcac gaaattgtat
     2041 tgagaggtag ttcaaatggc aatacgtatg tttatgatcc gtataaccgt gataacaatg
     2101 gtttttacag tgttgaccgc atttggaatg aacagtcacg agatagtatt gatactgctg
     2161 gtgtaggtgt accgtttttc tcaattatga ctaaagatat ggctaatgcc ttaactaagc
     2221 agtcacaagc tttagcaagt caacaagttg ctcaaagaca attaaatgat gcacaagcta
     2281 aagcaatagg tcttaatgca gtaactatgc agacaccgat tgcacaagct aatttgatta
     2341 aagcacagtc taatttaaaa gatgctcaag atgcactcac taaagcacaa gaagcagtta
     2401 aattagctaa tcaagataat gttaaaaaac aagctgactt aataaaagca gagtctaaat
     2461 taacagatgc tcaaaagcaa ttagcagatg cacaggttaa attgacaaca aatacaacta
     2521 aacttaatca attaaaacaa gtgttagcag aagcaagtca acaagtagca caagcaaatc
     2581 aagattacaa gcaagctaaa gataatctaa cacaaaaaac tgcttatctt acaaatctac
     2641 gcaatgcaca agctaatttg cttaaagtac agtctgatgt agcacaagct aaagataact
     2701 tagcaaataa gattgctaag ttacaaagag aagtagctta tttacaagag ttaaaaacta
     2761 aagcagtaga tgcgcaatca caatatcaaa aagttttatc agcttataag tcagttttag
     2821 aagctaaaga aaaactgaaa ttagcagaag aaaaagctca aattgagaaa gtaagacaag
     2881 aagcagtagc agtagttgat gaaacaggta aaattactag ttatattact tctaaaccaa
     2941 aactgcaaga agctgttaaa gcacaacaaa tagttgttgc taacgataag aaagacgttg
     3001 taactaagac agttgatgtt gaacaagtat cagttgtaaa atctagtgtc ttgccatcaa
     3061 ctggtgatgc taagacaggt ttaattgcag ttttaggtat gctgttatct ttaagtgggt
     3121 ttttaggtgt acgtaaaaat aataagaata atatttaata ataaggagga tgatttatgt
     3181 gtgatatttt gagggcaaga tcgccttctt ttttttatat aaaaaattaa aatcaaagga
     3241 gaaaatatgt ataaaaatca aaacacaaaa ggtcacggat tttttagaaa atcaaaagca
     3301 tatggtttag tatgtgggat tgcacttgca ggtgcattta cattagctac tagtcaagtg
     3361 tctgctgatc aagttacaac tcaagcaaca actcaaacag taacgcaaaa tcaagcagaa
     3421 acagtaacat caactcaact tgataaagca gtagctacag ctaaaaaagc agctgtagct
     3481 gttacaacca cagctgcagt taatcatgcg acaactactg atgcacaagc tgatttagct
     3541 aatcaaacac aaacagttaa agatgttact gcaaaagcac aagctaatac acaagctatt
     3601 aaagatgcta ctgctgaaaa tgcaaaaatt gacgcagaaa acaaagcaga gtctcaacgt
     3661 gtaagtcagt taaatgcaca aactaaagca aaaattgatg ctgaaaacaa agatgcacaa
     3721 gctaaagcag atgcgactaa tgcacaatta caaaaagatt atcaagctaa attagcgaaa
     3781 atcaaatcag ttgaagctta taatgcaggt gtacgtcaac gtaataaaga tgcacaagct
     3841 aaagcagatg cgactaacgc acagttacaa aaagactatc aagctaaatt agcactttat
     3901 aatcaagctc taaaagctaa agcagaagca gataaacagt ctattaataa tgttgctttt
     3961 gatatcaaag ctcaagctaa aggtgttgat aacgctgaat atggaaactc aatcatgact
     4021 gcaaaaacta aacctgacgg aagtttcgag tttaaccatg atatgatcga tggtgtgaag
     4081 acaatcggct atgggaaatt gacaggtaaa gttaatcatc attatgttgc taacaaagat
     4141 ggctctgtga cagcatttgt tgactctatc actctttaca agtacgagta tcgtaatgtt
     4201 gctcaaaatg ctgctgttaa tcaaaatatt gctttcagag tcttaactaa agacggacaa
     4261 atcatttttg agaagaaaca taatggtaac aaaacttttg cagaaacttt aaacaaaact
     4321 ttacaactca atcttaaata tgagcttaaa ccacatggtt ctagtggaag cgttgaagtc
     4381 tttaagattc atgatgactg ggtacatgac acacatgggt ctgctttagt gtcttatgtt
     4441 aataataatg atgttgttcc taacgttgtt ataccaaaac agccaacacc accgtcaaca
     4501 gaaaaagtga ctcctgaagc agaaaaacca gtacctgaaa aaccagttga gcctaaattt
     4561 gtaacaccta cattaaaaac ttacactcca gctcaaccaa aagtgaaacc acatgtgtct
     4621 attcctgaaa agattaacta ctcagttagt gttcatcctg ttttagttcc agctgctaat
     4681 ccttcaaaag ctgtcattga tgaagcaggt caatctgtta atggtaaaac cgtattacca
     4741 aatgctgaat taaattacgt tgctaaacaa gactttagcc aatataaagg aatgactgca
     4801 tcacaaggta aaatagctaa aaactttgtc tttattgatg attataaaga tgacgcacta
     4861 gatggtaagt ctatgaaagt gaactcaatc aaagcatctg atggcacaga tgtgtcacaa
     4921 ctcttagaaa tgcgacatgt gctttctacg gatactttag atgagaaact tcaaacgctt
     4981 attaaagaag caggaatcag tcctgttggt gagttttaca tgtggactgc aaaagatcca
     5041 caagcttttt ataaagctta tgttcaaaaa ggtctagatg ttacttataa cttatcattt
     5101 aaagttaaaa aagagtttac taaaggtcaa attcaaaatg gtgttgcaca aatcgacttt
     5161 ggcaatggat atacaggtaa tattgtagtc aatgatttga caactccaga aatacataaa
     5221 gatgtgttag acaaagaaga tggcaagtct atcaacaatg gtactgtcaa attaggtgat
     5281 gaagttactt acaaattaga gggttgggta gttccaacag gtcgttctta tgatctattt
     5341 gaatacaaat ttgtggatca attacaacgc acacatgatc tttacttacg tgataaagtg
     5401 gtcgctaaag ttgatgtgac attaaaagat ggtacagtca ttaaaaaagg gactaattta
     5461 ggagagtaca cagaaacagt ttacaacaaa aagacaggtc tctatgaatt agtcttcaag
     5521 aaagacttcc ttgaaaaagt tgcacgttct agtgaatttg gggcagatga ttttgtggtg
     5581 gttaaacgta tcaaagcagg tgatgtttac aataccgcag acttttttat caatgggaat
     5641 aaggttaaaa ctgaaactgt ggtaacacat actcctgaga aaccaaaacc agttgaacca
     5701 caaaaagcaa ctccaaaagc tcctgctaaa ggattacctc aaactggtga agcaagcgtt
     5761 gcccctctta cagcacttgg agcaatcatc ttgtcagcta tcggtttagc aggatttaaa
     5821 aaacgcaaag aaaattaaaa agtcaatatt cagaagcact tgaaaaagtg cttcttattt
     5881 ttagaaaaga ggaaattatg aaacaagaaa cattagtgtt acttccaaat tttattggag
     5941 atgcaattta tgaaggattt gaagctggac ctgctcaatt aaatggtaaa aaagggtctt
     6001 atacaagaca cattttgttt aatgaaaaac atggtaagtt ttatgtttat acaccagcat
     6061 cttcacgtgt gattggaaat ggaaaaatgg taactgttga aaaaccgtta ttgtttattg
     6121 acaatacaat taatggtaga aatattgaac ctgcaaaaaa tgtatgggct gaaaagctta
     6181 ttgaagtgaa ataaggaaag tagaggatta gaaatatggc aaaaattggt ttaaactttg
     6241 gagaaaaatt acaaattgca gataaggaat atcgtacctt taatgatgga ttggatgtaa
     6301 atgttttgtt aggagattta acttggagac gatatgaaga accagttcaa tttacggaag
     6361 atgatacgac acaaccacca gatcgatttg gtcgttatcc acaaaaacca acaggtgaag
     6421 tgaagtggaa tgatattgca gtatttagta ctgctcaaaa tgaaacaatt tttgtttcaa
     6481 ttactgatat gccttcttat gcaatcgagg atttaggtct taaattgggt gaccctattg
     6541 aattaagtga tgttgttgtg acatggtcta acgtttcagg tggtatgttt aaaatttttg
     6601 catcatcact taagaaaaag gatgttgcaa ataatggaaa cgcatcaaaa aatgaagcat
     6661 ctaaagttaa taaataaacg gtaaaggagg ggtaatttgg ctcgtttaat accacaatat
     6721 agagggatta aggtaaaacc atatatgagg tatatgagtt attacctttt ttcttttcta
     6781 tttattttgt ttttgacacc tgtaggcgtg tatagttatt actatttaga tttgttaaaa
     6841 atgatggata aaatgtctat gatcagtgta ggtacaggtt tgtttttagc attttttgtt
     6901 tcttggtatt tgacttggtt tttgcaagaa gctaatccat tatttaataa actagatcga
     6961 ttaaaaagaa tgtctaagtt tttatatgag aatggctatg tttatgaaaa acgtaaaaaa
     7021 tcaaataaga aaacgaagac aaaatatcgt tttccaaaag tttatgtgaa acaaggtaag
     7081 tatgatttaa gtgtttcatt tgaaatggct ggaggaaaat ttcaaaagaa atttaaggat
     7141 attggtggag aattagaaga tacttttttc atggatttta tggaaaaaac tgatgatcca
     7201 cgctttaaaa tttacaaatt agcttattcg gctttcttat cacgtattac ggttaaagat
     7261 gtgatatggg ataaagataa gggaattaag ttaatggatg gctattattg ggatttcatc
     7321 aatgatcctc atttattagt tgctggtggt actggtggag gtaaaacagt attactaagg
     7381 tcaatccttc gttgtcttgc ggaaattgga gtttgtgata tatgtgaccc taaaagagcc
     7441 gattttgtta ctatgtcaga tttatcagct tttgaaggta gaattgcctt tgaaaaggct
     7501 gatattattg agaagtttga aaatgctgtt acgataatgt ttgcacgtta tgattttgtt
     7561 cgaaatgaaa tgaagcgttt aggtcataaa gacatgaaaa agttttatga ttatggattg
     7621 gaaccttatt tttttgtatg tgatgaatat aatgctttaa tgagttcttt gtcttatcag
     7681 gaacgtgaga ttgtagataa tgcatttact caatatatat tgttaggacg tcaggttgga
     7741 tgtaatgcga ttatagcaat gcaaaagccg tctgcagatg atcttcccac taaaattaga
     7801 tctaatatga tgcaccatat ctcggttggt cgtctcgatg atggtggtta tgtgatgatg
     7861 tttggtgatg aaaatagaaa taaggaattt aggtttatta aatatcttgc aggtcgtcgt
     7921 gtttatggtc gaggttattc agctgttttt ggtgaagtag caagagaatt ttattctcct
     7981 ttattaccta aaaatttctc tttttatgat gcttttgaaa aaataaatag gcatgagaat
     8041 ccttttgatc ctacagaaaa tcaggaagtt tctaaggcga ttcttaaaga cgaatctttg
     8101 agagagtttg tggaaaaaac gtctgaaaat gagttactta agggttcagt agggtttgat
     8161 tttgatgatg aaatggagga aatggaaaat atgagtattg gtgatttagc taagaaaaca
     8221 ggtttttcat ttggtaagat taagaattta atagatatga ttgaaaaaga taattatatg
     8281 acttttagtc gtgaagatga aaaggttgta ttaaatgatt ctgatgcagt cgtcttagaa
     8341 agtttactga cacaaaaaga tgaatttgag ggtagctgga aagagttact attgaattat
     8401 tttaagggtg aatagtagat gaagggtatt ttttaaatat ccttctgtaa agccctagtg
     8461 tagctgtagg cgtagtgtag cgtagcgaaa acggacacgc cggacacagc gcacacacta
     8521 gggctttaca gagggattta tcaattttaa aacccccgtt ttctaatagg ggggtaagaa
     8581 atgaaaaatt tcgaatttta acccccaaaa acctatatgt atcaagggtt ttcagatttt
     8641 taaaggtggg tcattttagt gaaaaaagtg ggtcactaat cctaagaagg agtttgttta
     8701 ttgtggatgc tatctattta aaaaaatttc gaaagaaaac aggtctaaaa caaaaggaat
     8761 ttgctttaag ttctggtttg acattgaaat tacttagaaa ctatgagcaa ggaaagcgga
     8821 agttgacact tgaaaagtat caagagataa aatcacattt tggatatcta gtagaaaatg
     8881 atagttcacg attacaagtg atgattgact atgtcagaat tacgttaaag gatgttaggg
     8941 atcttgaatt tttctgtcgt aactttttgc actgtgcatt taaagaattt cagccttttg
     9001 aatcgaagtt gatgaattac aatcatcttt ggaaacgtgg agatatttgg atatttgatt
     9061 ttgcggataa acatgagaca ggtaattttc aaataactgt tcaattgtct ggtcgtggat
     9121 gccgacaatt ggaattgttg atggaaactg aaaaatttac atggcatgat tggttaagtt
     9181 atctaagaaa ttcatatcgt gatgatatga atgtgacacg ttttgatatt gctattgatg
     9241 aattgtatct gggtaaggat agagaaaatg aacagtttca tttgtcagat atgatttcaa
     9301 aatattatcg tcatgagtta gattttgaaa gtttgagaac ttggaattat ataggtggcg
     9361 gttctttaaa tttttcggat atggaagaga ttgagcagaa tagacaagga atatcgcttt
     9421 attttggtag tcgtcaatca gaaatgtatt ttaactttta tgaaaaacgt tatgaaattg
     9481 caaagcagga aggaattact gttgaagaag ctttagaaat ttttgaattg tggaatcgtt
     9541 atgagatacg gttatctcaa agtaaagcta atgcggcagt tgatgaattt atttctggag
     9601 tgccgattgg tgaaatatcc agagggttaa ttgttagtaa gattgacgtg tatgatggga
     9661 aaaatgaata tgggtcgttt caagcagata gaaagtggca attaatgttt ggaggtgttg
     9721 agcctttaaa atttgttaca aagccagagg cttatagtat tgaacgaacc ttacgttggt
     9781 taagtgattc tgtttctccg tctttagcta tgataagaga gtatgacatg attgtggatg
     9841 gtgactattt acaaaccatt cttaattctg gggaagtcaa tgaacgtggg gagaagatac
     9901 ttgacagtat caaagctagc ttaggtattt tgtaggaggt aagttttgtt ttgtatagta
     9961 accgagaatt tgcttatttt gttaatagac gaaataattt agataaaatg attgatttac
    10021 tagtttttat gattcctgat cgtgagtttt attatcctga gattcaaaca ggtgagctta
    10081 gagattatca gatagacatt tatgatttga ttaagattgg ttatgttggt gtttatgaaa
    10141 ttcaaaaaga ctacgaagat aagttaaggg aacttgctga ttttaaacgg aaacttttaa
    10201 agtttggttt gcttatgcaa cctttagaaa agcaaaaaga gatagttatt cgtttagctg
    10261 gtaaatatcg tttggaaaaa agaattctga tgagaaggga aatgtttaga gatgaagaag
    10321 ttgactaatt ttccacaaaa actaaaagaa ttgcgtaaag aaaaaaaaac taactcaaac
    10381 tgagctagcg tctaaattaa atataagtca aaagtcatat agtaattggg aaagtggtaa
    10441 agctgaacct actttagata atataataaa gttagcaaat atcctagatg ttactgtaga
    10501 ttatttactt ggtagaagtg ataattttag taatactatt gtgctttcaa aaaacaatat
    10561 gaaaagtttt tctaaaagac tgaaagagtt aaggttagaa aaagaatatt gatgaaaagg
    10621 gaattatttc gagaatatga aactgactaa tttgaggaag gtttggatga cttcagaata
    10681 atc
//
LOCUS       DATROY010000066        10538 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.067, whole genome shotgun sequence.
ACCESSION   DATROY010000066 DATROY010000000
VERSION     DATROY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(27..1499)
                     /locus_tag="VBD11_001287"
     CDS             complement(27..1499)
                     /locus_tag="VBD11_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954238.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(1730..2566)
                     /locus_tag="VBD11_001288"
     CDS             complement(1730..2566)
                     /locus_tag="VBD11_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6954239.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(2566..3936)
                     /locus_tag="VBD11_001289"
     CDS             complement(2566..3936)
                     /locus_tag="VBD11_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6954240.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4034..5281)
                     /locus_tag="VBD11_001290"
     CDS             complement(4034..5281)
                     /locus_tag="VBD11_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6954241.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            5537..6565
                     /locus_tag="VBD11_001291"
     CDS             5537..6565
                     /locus_tag="VBD11_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6954242.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            6686..8182
                     /gene="malQ"
                     /locus_tag="VBD11_001292"
     CDS             6686..8182
                     /gene="malQ"
                     /locus_tag="VBD11_001292"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6954243.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8194..10458
                     /gene="glgP"
                     /locus_tag="VBD11_001293"
     CDS             8194..10458
                     /gene="glgP"
                     /locus_tag="VBD11_001293"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6954244.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3597 a         1694 c         1973 g         3274 t
ORIGIN      
        1 tgtaacctag ttctttttag atgtctttat ttaactccag acattagttt ttcaatgcgt
       61 ggaacataaa ataagaggat aaatccaaat acaactgtta taccaccaac aacaccataa
      121 taaagaattt gagtatcagg tgtgtacaat ttaacgattt gagcgttaat agcttgtgct
      181 gcagcattac ttaagaacca gatgctcatc atttgagctt ggaatgcttt aggtgcgagc
      241 ttactagttg ctgataatcc aactggtgag attagcattt ccccaacgat gacaatagcc
      301 caactcattg ttaaccaaag agggcttact ttagcgttaa ctccaaagag taaacctggt
      361 agcatcatcc ataagaatga agctccagca aagaaaagtc catacgcaaa ttttttaggt
      421 gatgaaggtt gcttacttcc ccatttagcc cataaccaag caaagaatgg tacataaagc
      481 ataatgaaga gagggttcat actttggaaa taacttgatg ggaaattaat atgatgccca
      541 aagaagttaa ggtaaagttt tgtttgttca tctgcaaata aggctaagac aactgaacct
      601 tgttcttcaa ttgaccaaaa tagaattgag gcgataaaga gagggatata tgcccacact
      661 cgagaacgct caacagaaga aatcttttta ctacttaaaa tcttgataaa ataaataatt
      721 gggatggcga ttgcaataat actaaaaata tcgataatta cttcaattgt tagcatatgc
      781 atcaagtgaa gtagaataag tacaataaca ataccaataa ggataagccc aactttttta
      841 tatagagagg acttttcttc aggacttaaa ggatcgtttg gtctgagact ctcttcagtc
      901 aaatattttt ttccatctaa ggtatattgg aggagaccaa agaacatacc aatagcagca
      961 agtgagaaac caagatggaa atttacttct tgtccgaggt accccactac aatgggtgaa
     1021 ataaaagcac caaggttaat accaaaaaca aagatactaa aaccagcatc acgacgagaa
     1081 tcattttctc catataaatt accaaccatc tctgatacat ttggttttaa tagtccagta
     1141 ccaaggataa ttagagcaat cgaaatgaat agagctgttt gaccaaatgg tgtagctaga
     1201 gcaatatgac ctagcatgat tagaatacca ccatacagaa cagttttacg actacctaga
     1261 atacggtcgc tgacaaaacc accaattact gatgagagat aaaccagtga accatagaca
     1321 gccatgattg atgcagcgac agtcttgtcc ataccaaggc caccttgaga aacactatag
     1381 tacatatagt acagcaaaat agcacgcata ccatagtatg agaatctttc ccacatttca
     1441 gtaaagaaaa gagtggacaa accacgagga tgtccaaaga atgattttgt tttttccatg
     1501 aaaaacgctc cttttgtaga taacactatt aataatatag taaatagaag caactgtcaa
     1561 attacttagc actatcaagt tatttttttg tagaggttaa cacccttggt aaaccaagtt
     1621 ttttaactat ctagcttaga aaaaggcttg aattcaatag aacccaagcc ttttttagaa
     1681 aagaaaccac agtgaaataa aaatgtcgtg tctttaaatt atctgaagat tattttacag
     1741 atccacctgt aataccttca acatagtatt tttgcatgaa catgaaaagt attgtgattg
     1801 gaattgcaat aataacagat cctgctgtaa atgacatgaa ccactggttg atggtatctt
     1861 gttgcaacat tgaaaagaga ccaatagcga ctgtgtactt gctcgtagca tcacctaaaa
     1921 taacttttgc aaagataaag tccatccaag ggcccataaa agcgattaat gcagtataaa
     1981 cgataatggg ttttgacaat ggtagggtga ttttaaggaa aatatccaaa cgggtcgccc
     2041 catcaatcat agcagactca tccaaagagt atggaatagt atcaaagaaa ccttttgcga
     2101 tgtagaaggt taatgctgct cctgcagaat agacaaatat taaagcggtt aatgtttggt
     2161 ctagatttag agcttttagg atgtagtaaa ctgcaatcat tgacatgaac ccaggaaaca
     2221 tattcaagac taaagctagc tttaggaaac cattacggtg tttgaatttg attctgctga
     2281 gagaataagc cattgctaca gtgattaaag ttgatagtat acaagtacat gtagcgacaa
     2341 ataatgtatt taagaaccat tgaccaaatg ggaatgtgtt ttgggtgaag agtttagcgt
     2401 agttgtctaa tgtccaagtt ttaggaataa agtagttgac aaaggcactt ccttcaccac
     2461 ggaagcttgt gagaacgacc caaacaatgg gaaagagcca cataattgat agtactatca
     2521 ataaaatata aacaaaagta aggttaagac gttttttctt attcattatt tagctgcccc
     2581 ttccttgtaa gaatttgttc ttgtgtaagc tagtaaacta aatactgctg aaagaataaa
     2641 gattaagata ccaacaacag atgctaagtt ataatctgca gcagttactg tcaaattgta
     2701 tagccaagtg acaagtaaat cggtgctacc tgcttggtag tatgttgagt ttgttggtcc
     2761 accttgagtg agtaggtaga taacgttgaa gttgttaata tttccgataa attgttgaat
     2821 taaagtagga gtcataatca acaaaatttg tgggaaagta attgattgaa agacttggaa
     2881 cttattcgcc ccatcaattt cagcagcttc aatttgttct gcaggtaaat tcatgatgat
     2941 accagtagca acaagcattg ttacaggaat accaacccac atattaacaa agatgattga
     3001 gaatttcgcc cagaccggat cagaaaggaa tggaagggga tgtgctgaag aaataagtcc
     3061 ccattttatt aacaaggcgt taaccggtcc agcgtcactt aagaggtttc tcatgattaa
     3121 gagagagata aattgaggga cagccatggt aataacaaaa atagtacgcc acatcttttt
     3181 gaatttcaaa ccttttgtat taattagtaa ggcaagaata ataccaaaga agaaattcgt
     3241 tacagtagcg aaaacagccc aaattaatgt ccaagataga acagggaaga aagtactggc
     3301 catacgcccg ctaaagatat taccgaagtt agcaaaacca acccagtcaa ataaactttt
     3361 tggcggtaag tggttatgat catagttagt aaatgccaaa caaatcatat aaactaacgg
     3421 aaggattgta aatagaagca cccctattaa aggaatagac attaaagtca tatggaagcg
     3481 tccatctgtc aagcttttaa tatctttttt gaaggatgga atttctcttc cttcttcttt
     3541 caaggtcaat aaatgtgctg cgcttttaat gtttgaccag taaatatagg caaatacgca
     3601 acagaagata agcgaagcta atccaaaaat tagcattaac atagagttat caccttgtgt
     3661 cgcaacttgg attttaatac catccacagt tttagttgtc ataccttgtt cttgggtccc
     3721 taaggaaata agacctttta cagctgggat aacttggtaa acaaaggtaa ttaaaaacag
     3781 aatttcactg attaaaaata aaagtccctt gacaatttgt ttatttttga ggttagcaag
     3841 tcccataatt aagaatgata gtttagttgc taaatcacct ttttgaaaga cctcttttag
     3901 ggacatacca tcaagtgaga tcagtttttt attcataatg gtctccttta attgttattt
     3961 tacaaaataa gagatgggaa gcactctcaa ttgaaaggaa gagtactcac ctcatctcat
     4021 tactctagac gtactatttt gttttagcga tgtctttatc gaattgtttt agacgtttaa
     4081 ggtaatcgct agatttgatt ttaccactgt aagtatcgct aagaatagca gcactttctg
     4141 tccagaatgt tgacatttga ctcaacttag gcataaccgt tgtatattta tctgagctac
     4201 ccatttcgat aactgctttt gcaagttcat ctttttggac gctatcagaa gattgaattg
     4261 atgagttagc aggaacgata tgacgttttt cgaattgaat tttttgactt tcagcattag
     4321 ttagatatgc agcgagtttg tagctagctg agattcgttt agtgtttgaa ccagcaggtg
     4381 cttggttaac ggcataaagt ttaacgccca agaatgcttt ttgttgaact tctttgtcac
     4441 cgattttcat tgttgggtaa acagcaacac cgattttatc ttcaccgaca gcttttttag
     4501 cagcgtcgta atcccatggt ccactttcaa aagcatgaac agaaccatcg ccaaatttag
     4561 acattgtatt ttcagctgtc aagttgacaa aaccatcatt tttcttttga tctgcaatcc
     4621 atttaaggac agaaacacct gcttcattac cccagttagt gcctttagca tcttcaccag
     4681 atttaccaaa taaagtgtcg cctacagaaa ggaaaagagg acctgttaca tatgagttag
     4741 ctgctttaag ctgttgaccg aatttccctt tgcttgtaat tgtttcgtat gatttaacgt
     4801 cgtcagcagt taactttgtt ttattataat aaagaacttg agattcaata ccaaatggga
     4861 atgcataagt tttcccttta tattgtgcac cagtaagtga ttgtttagtg tcgtttttag
     4921 caatttcttt tgagtattgc tctggaattt cttggataac accagattct actaattgac
     4981 caagttggtc atgtggaagt gagaatacat cggctgcctt gcttgggtct ttttttacgt
     5041 tttcttgagc tttggagtca ttagactcaa tcatttttac agtaacgcct ttgttttcct
     5101 tctcgaattt tttaacaatt gctttataag acgcttttga atctgttggg acccaaagtt
     5161 tgatagttgt ttttgaagca gcatcaactg agttagagtg agtagcagca attgctcctc
     5221 ctgcaactac tgaaagagca gcagtactaa caagtaattt tttccaagta tttttttcca
     5281 ttttggttct cctcctagag aatattttaa ttacacctat attatgcaac cgtttgcaaa
     5341 aaaagtcaag tcttttttta aaaaaaataa gaaaaataaa aaaataatat caattaatac
     5401 taaatgagaa gattatttta gtgagagtat tacatttgca gagaaggcaa acgtttgttt
     5461 ttttcttaga ttagagtata atagacaaag gactatacca aaaggtataa aatgattaga
     5521 atgaagaggt cgtaggatgg taactataaa agatgttgct gccaaagctg gtgttaatcc
     5581 atcgacagta agtcgtgtac taaaagataa tgaatctatt tcaagtaaga cgaaagagcg
     5641 cgtgaaaaaa gctatggaag agctgggcta tgttcctaat gttgcagcac aaatgttggc
     5701 tagtggttta acgcaaaata ttggagttat ttttcctcca ctggttttac cagaccgctt
     5761 aagtgaaccc tttttcatgg agattctttc tacattaact aatgaagcaa aagagcatgg
     5821 gtttacagtt tctattgcaa caagtatgtc ttctgaggag ttgttagaac aagtaaaatt
     5881 aatgcatcta caaaaacgag tggatggttt tattgttctc tattcggatc aaaaagatcc
     5941 tatcagagag tatttattaa aatataaact gccctttgtt gttgttggtg ctgctaatgg
     6001 ctatgaaaat gagattactt atattgataa tgataatcag ctgatgggga aatctgccat
     6061 tgattatctc tataaaaaag gacataaaag catccagttt gttacagatg atttaaattc
     6121 agaagtatca gaagagcgct atttagggta ttttaaaggt gctagaaagt taggtttaaa
     6181 ccaaaagcca gctcttttat ttgacagagg caatccgcaa gttttagagg aatttataaa
     6241 tagagtcaaa gaagaagaaa caactgcttt aattgttatt ggtgatactg tctctgttag
     6301 agtaatgcaa tttctttctt tctataaatt aaaagttcca gatgacattt ctatcatgac
     6361 ctttaataat tctctcttct cacatctgat tcatccttac ctaagcacat ttgatattaa
     6421 tgttaataat ctaggaagga caagcgttcg tcgtctgatt gacatcatta aatctccaga
     6481 caaagtcttt agtgagacta ttattgtgcc ttttacttta gaagagcgtg aaagtgtccg
     6541 agatatttct aaaaataata attaagctat gatataaaga cttctatcaa ttttcgatag
     6601 aagttttttt aaattatgct tgacttatgc aatcgcttgc gttaagatgg aggagtaaag
     6661 taagtaatcg aaaaggagaa tcaatatgaa aaaacgtgca agtggtgtct taatgcacat
     6721 cacttctcta ccaggtgatt taggtattgg aacatttgga cgagaagcct acgcttttgt
     6781 tgattttttg gtcgaaactg atcaaaaatt ttggcaaatt cttcccttaa caacaacaag
     6841 cttcggggac tctccttacc agtctttttc tgcagtagct ggtaatacac atttaattga
     6901 ttttgatttg ctaacgcttg aaggtttcat ttcgaaagat gactaccaaa atattagttt
     6961 tggacaggat ccagaagttg ttgattatgc tggtctgttt gaaaaacgcc gtccagtttt
     7021 agaaaaagca gttaaaaatt tcttgcaaga agagagagct acgagaatgc tatctgattt
     7081 cttgcaagaa gaaaaatggg taactgattt tgctgaattt atggcgatca aagaacattt
     7141 tggtaataag gcgcttcaag aatgggatga caaggctatt atacgccgcg aagaagaagc
     7201 cttagcagga tatcgtcaaa agcttagtga agtgataaaa tatcatgaag taacgcaata
     7261 tttcttttac aaacaatggt ttgagttaaa agaatatgct aatgataaag ggattcaaat
     7321 tatcggtgat atgccaatct acgtttctgc cgatagtgta gaagtttgga caatgcctga
     7381 actgtttaaa ttagattgcg acaaacaacc acttgctatt gctggtgtac cagcggatga
     7441 tttttctgac gatggtcagc tttggggcaa tcctatttac aattgggatt accacaaaga
     7501 gtcagatttt gactggtgga tttatcgtat tcaatctggt gttaagatgt atgactattt
     7561 gcgtattgat cattttaaag gtttttcaga ttattgggaa attcgaggag attatcaaac
     7621 agctaatgat ggctcatggc aaccagctcc cggtccagaa ttatttgcaa ctatcaaaga
     7681 aaaattaggt gatttaccta ttattgctga gaatttaggc tatattgatg agagagcaga
     7741 gagattactg gctggaacag ggttcccagg tatgaaaatc atggaattcg gtttttatga
     7801 tacaacagga aatagtattg atattcctca taactacaca gaaaatacaa ttgcatatgc
     7861 tggaactcat gataacgaag ttattaatgg ctggtttgaa aatcttacag tcgaacagaa
     7921 agcttatgca gaaaattaca tgcgtcgttt gccaaatgag cccattactg agactgtact
     7981 gcgtaccttg tatgcaaccg ttagtcaaac aacgattaca tgtatgcaag atctactaga
     8041 taaacctgca gatagccgta tgaacatgcc aaataccgtt ggaggtaact ggcaatggcg
     8101 tatgagaaaa gaagacttaa cagaaaatcg caaagcattc ttaaaagaga ttacaactat
     8161 ttataataga gggaataaat aaggaatatt atcatgacaa gaaattttac aacttacgtt
     8221 ggacaacaag gaaaagtatt atcagaatta acaaatgaag agatttacgt tgaattgtta
     8281 aattttgtaa aagaagaagc tgccgctaaa tcaaaaaata gctctcaacg taaggtatac
     8341 tatatttcag ctgagtttct aattggtaaa ttactttcaa ataatttgat taacttaggg
     8401 atttataaag atgtcaaaaa agagttagaa cttgtaggta aatcaattgc tgaaatagaa
     8461 gatgtagagc cagaaccatc acttggtaat ggtggtttag ggcgtttagc atcatgtttt
     8521 attgactcga tttcttcttt aggtattaat ggtgaaggtg ttggtttaaa ctatcactgt
     8581 gggcttttca aacaagtatt tcgcaacaat caacaagaag cagaagctaa ctactggatt
     8641 gagaataatt cttggttagt accaacagat atttcttatg atgttccttt ccgtgacttt
     8701 actttaaaat cacgcctaga tagaattgat gtattaggtt ataagaaaga tactaaaaat
     8761 tatttgaacc tttttgatat tgatggactt gattataacc ttattgaaaa aggtatcact
     8821 tttgataaga cagaaatcaa gaagaattta accttgttct tgtatccaga tgattcagat
     8881 aaaaatggag aattattacg tatctatcag caatatttca tggtctcaaa tgctgctcaa
     8941 ttattaattg atgaagcaat cgagcgtggt tcaaaccttc atgatttagc tgaatatgct
     9001 tatgttcaaa ttaatgacac tcacccatct atggtcatcc ctgagttgat tcgtttattg
     9061 acagaaaaac atggttttga atttgacgaa gctgtgtcag ttgttcgcaa tatggttggc
     9121 tatacaaacc ataccatcct tgcagaagct cttgaaaaat ggcctttaga gtatcttaat
     9181 gaggtagtac cccacctagt aacaatcatt aaaaaattag atcaaatgat tcgtgaagag
     9241 caaacaaatc cagaggttca aattattgat gaagctggac gtgttcacat ggcgcatatg
     9301 gatatccact tctcaacaag cgttaatggt gtagcagcat tacatacaga aatccttaaa
     9361 aatagtgaat taaaggtttt ctatgatatt tacccagata agtttaacaa taagacaaac
     9421 ggtattacat tccgtcgctg gttggagttt gctaaccaag atttagcaga ttacttgaaa
     9481 gaactaattg gggatagcta tcttactgat gcgactcagc tcgaaaaatt gttaacttat
     9541 gctgatagca atgaagttca tgataaatta gctgctatta aatttaaaaa caaactggct
     9601 cttaaacgtt accttaagga aaataaaggt attgaattgg atgaatactc aattattgat
     9661 acacaaatca aacgattcca cgaatataag cgccaacaaa tgaatgcttt atatgttatt
     9721 cataaatact tagaaattaa acgtgggcat ttcccatcac gtaaattaac agttattttt
     9781 ggtggtaaag ctgctccagc ctatactatt gcacaggata tcattcactt aattctctgc
     9841 ttatcagaat taattaataa tgatccagaa gttaataaat accttaatgt ccatttagta
     9901 gaaaattaca atgtgacagt agcggaaaag ttaatccctg caacagatat ttcggaacaa
     9961 atttcattgg cttccaaaga agcatctggt acaggtaaca tgaaatttat gttgaatggt
    10021 gctcttactc ttggtactat ggatggggca aatgttgaaa tagctgaatt agctggcaaa
    10081 gagaacatct acacatttgg taaagactct gatactatca ttaatcttta tgaaacatct
    10141 ggttatcgtt caaaagatta ctatgataaa gacaaagtaa ttcgtgaagc tgttgatttt
    10201 atcattagtg atgatatagt gtcactcggt aatgcagaac gactaaagag attgcatgat
    10261 gagttagtag gtaaagattg gttcatgaca ttgattgatt tgaaagaata cattgctgtt
    10321 aaagaacaag tgcttgctga ttatgaagat tatgaatcat ggaataagaa agttatacac
    10381 aatattgcta aagcagggtt cttctcatca gatcgtacaa tcgaacaata taatcaagat
    10441 atttggcatt caaattaaaa agtctctaaa agtaaggttt tcaaccttac ttttttcttg
    10501 tctaataaaa ctataagtat ttatttttgt ccgtagac
//
LOCUS       DATROY010000067        10229 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.068, whole genome shotgun sequence.
ACCESSION   DATROY010000067 DATROY010000000
VERSION     DATROY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10229
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            121..1658
                     /locus_tag="VBD11_001294"
                     /pseudo
     CDS             121..1658
                     /locus_tag="VBD11_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1835..3379
                     /locus_tag="VBD11_001295"
     CDS             1835..3379
                     /locus_tag="VBD11_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6954245.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3465..3845
                     /locus_tag="VBD11_001296"
     CDS             3465..3845
                     /locus_tag="VBD11_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6954246.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            4039..4773
                     /locus_tag="VBD11_001297"
     CDS             4039..4773
                     /locus_tag="VBD11_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6954247.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            4766..5428
                     /locus_tag="VBD11_001298"
     CDS             4766..5428
                     /locus_tag="VBD11_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954248.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            5444..6274
                     /locus_tag="VBD11_001299"
     CDS             5444..6274
                     /locus_tag="VBD11_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954249.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEADVAF
                     "
     gene            6509..8095
                     /locus_tag="VBD11_001300"
     CDS             6509..8095
                     /locus_tag="VBD11_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6954250.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(8278..8544)
                     /locus_tag="VBD11_001301"
     CDS             complement(8278..8544)
                     /locus_tag="VBD11_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6954251.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(8537..9301)
                     /locus_tag="VBD11_001302"
     CDS             complement(8537..9301)
                     /locus_tag="VBD11_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6954252.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            9436..10176
                     /locus_tag="VBD11_001303"
     CDS             9436..10176
                     /locus_tag="VBD11_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6954253.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
BASE COUNT         3360 a         1570 c         1997 g         3302 t
ORIGIN      
        1 tcatttttag ctatttttat gattgaccgc taattattaa tctgctaaga tatagtacgt
       61 aataaaaact taaccggtta attatttttt gaaatatatt ttttctggag gaaaatagta
      121 atgaataaat cattcaatac caaattaggt ttggtaactg tagcggcatt atcaggtatt
      181 gtattaacgt ctcaattacc tgtgaacgct aaagctgaga caccagtaat ggcagcaagt
      241 gcacaacaag gtttccgttt tgtagcaacc gttgtagata gtcagacgcg tgtcttgccg
      301 ggaaaattgg taactttgtc agaggtgaca actggtcagc ctaaaattat tgcaagtgta
      361 aaaacgaatg atgccggaca agctattttt aataatctcc ctataaaacg taaccttagt
      421 gtgtctgttg atggacaagt aaaaggatat actattcgaa ctgacgtagc aggctctagt
      481 aaagcagcta gttttacagc aacaggagta gggacaaatg agccaacgta ttcaaaaaaa
      541 accatagaca ttacagtgag agaccaaaat gctgaacctg tttcaggcag gacagttacc
      601 ttaaaaacac atgctggtcg tgagattgct agcttggttt ctggtgacaa tggattaact
      661 cgctttactg atcgtttact agatggaaca ttctaccaat attttgttga tgggaaaaaa
      721 ttggtgatat tgtacctgga gaatcaagaa gtgcatatgt taatgttgca cctaaaaaag
      781 attattttac ctttacagtc actgctttag ataaagatgg acttgttgtt aagggaaaag
      841 aagtaacgtt gactgatatt actgatggta aagcagtagc gcttgcttct ttaaaaacga
      901 atgataatgg acaagctatc tttaccaacc taccattatc aagaaatatc agtgtttcaa
      961 ttgatggaaa atcaaagggc tatactcttc gaactgatgt agcaggctct cataaagcgg
     1021 ctgcattcta tgtagatggt aaaggtacca aagcacctca atttagtgca gaagcagcgg
     1081 ttgtaactat ttacgatgct aatggaaata ctatagcaaa ccaagaagtg acattaacta
     1141 attcaaatgg gacaattgtt gcaaaaggtt tgactgaaaa agatggcaaa gcaaggttcg
     1201 ccaataaatt aatgggagga acgttataca atatttttgt aaatggtatt gagatgccta
     1261 aaactgccct tgttggtagt gatgtcagtg tttttctaac agataaacaa attaagaaag
     1321 aaaaacctgt tactcctgat aaattggata aagaaatgaa ccaagagaag gaaaaggagc
     1381 tcttagatac tccaaaacca gtaccaccta cttcgaaacc aacagcacca attaaaaata
     1441 aaaaggacga ttcccctaag aagtttacga aaagtcaacc ttctagtttg aagtcaagtg
     1501 ttaaggctat aggtgctaag ccaaaagcta tgacagcctt agctaaaaaa ttgcctaaaa
     1561 ctggtgatca agccattagt attttaactt tggttggttt tattgtaaca gggttagcga
     1621 ttatgttagg cttctttaaa aaatcacgat caaaataatt tattttagaa gcttgggtct
     1681 ttagacacag gcttctcttc tttcttcgga aatagattga acttgaagaa tgttgttaaa
     1741 ttcgctataa taataaagct atagacaaga ttattgtttc agttgagact actgaaacta
     1801 atactaagaa atggataaaa gctaaggatt gaatatgaca ttacaagacg aaatcaaaaa
     1861 acgccgtact tttgccatca tctctcaccc ggatgctggt aagacgacta ttactgagca
     1921 attattatat tttggtggtg aaattagaga agcagggaca gtaaaaggga aaaaatcagg
     1981 tacttttgca aagtccgact ggatggatat tgaaaagcaa cggggtatct ctgttacttc
     2041 atctgttatg caatttgatt acgcgggtaa acgtgttaat attttggata cacctggaca
     2101 tgaggatttc tcggaagata cataccggac cttaatggct gtggatgcag ctgtcatggt
     2161 tgtagattcg gctaaaggta ttgaggctca aactaaaaaa ctttttgaag ttgttaagca
     2221 tcgtaatatt ccggttttta cgtttattaa taaattggac cgtgatggac gtgagccact
     2281 ggatttgctt gaagagttag aagaagtact aggtattgct tcttatccaa tgaattggcc
     2341 aattggtatg gggaaatcat ttgaagggct ttatgattta cataataaac gtttagaact
     2401 ctataaagga gacgagcgtt ttgcaagtat tgaagacggt gatcagctgt ttgccaataa
     2461 tccattttat gaacaagtca aagaagatat tgaacttctt caagaagcag gtaatgactt
     2521 ttcagaacaa gctattcttg atggtgacct aactccagta ttctttggct ctgcccttac
     2581 gaactttgga gttcagactt ttcttgatac tttcttggag tttgctccag aacctcatgg
     2641 tcacaagact acagaaggta atgttattga ccccttagct aaagattttt caggttttgt
     2701 ctttaaaatt caagctaaca tggacccacg tcatcgcgac cgtattgctt ttgttcgtat
     2761 tgtttctggt gaatttgagc gcggcatggg agttaatttg acacgcactg gtaaaggcgc
     2821 taagctctcc aatgttactc aatttatggc agaatcacga gaaaatgtga cgaatgctgt
     2881 tgctggggat attatcgggg tttatgatac aggaacttat caagttgggg ataccttaac
     2941 ggttgggaaa aataaatttg aatttgaacc attaccaact tttaccccag aattatttat
     3001 gaaagtatca gcaaaaaatg ttatgaagca gaaatcattc cacaagggta ttgagcaatt
     3061 agtacaagaa ggagcaatcc agctttataa gaattatcag acaggcgaat acatgctggg
     3121 tgctgtgggt caacttcagt ttgaagtttt taaacatcgt atggaaggtg aatacaatgc
     3181 tgaagttgtt atgacaccaa tgggtaaaaa gactgtacgt tggattaact cagacgattt
     3241 agacgaacgc atgtcctcaa gccgaaatat tttagcaaaa gatcgttttg accaaccagt
     3301 tttcctattt gaaaatgatt ttgcacttcg ctggtttgct gataagtatc ctgatgtgaa
     3361 gcttgaagaa aaaatgtaaa atttaaagtc actcaaattt gagtgacttt ttaagttttt
     3421 tagactaaaa tagttataat acatagaaaa gattggagat taacatggga attttttcgg
     3481 gattaatggg aaatgcttca caagtggata ccgataaagt cgagaatcag ttatctgata
     3541 ttctgattcc aggtgaacag gttgatttag cttatacttt aattcgtgat ttgattgttt
     3601 ttactaatta tcgtttaatt ttagtcgata aacagggggt gacaggtaaa aaggtttctt
     3661 ataattctat accttatgct tctatatcac gttttacagt ggaaactagt ggtcattttg
     3721 atttagatgc tgagttaaag atttggatat catcagcaat agaaccagca gaagtacttc
     3781 agtttaagaa cgaccgtaac atagtgagta tacagaaagc tttggcgata gctgttttaa
     3841 aataaacaat ttagcaagtg ctgctactaa gagttatttg ttttaattat aacttttaat
     3901 agtgctaaat tctcattttc aaaataaaag tagtgatttt tatcactgct tttattttga
     3961 agtatagtag gcctcaagca tatattttga aaacgctgta tttttttgat aagatatttt
     4021 tttgaggagt attaaataat gataatagaa ttaaagagtg ttgggaaaat ttttccaaaa
     4081 tcacagttta gattagatga tatttctttt aatatagcag aaggcgagat agttggtctg
     4141 attggttcta atggtactgg aaagagtact attttgaaga tgattaatgg cttaatccct
     4201 tatgataaag gaaatattta ttatcaagga aaagaagtga agtctttttc tgataataag
     4261 ttacgtcaga tgcgtaaaga tattgcttat atttttcaaa atcataattt gttagcagga
     4321 gaaagtgtat attatcatct agctttagtt tataaattaa atcatcaaaa agtaaatcat
     4381 gacgcaatta atgatatctt agatttttta ggtttgatgg atttaaaaca ggtgaaatgc
     4441 catagtttga gtggtgggca gcaacaaaaa gttgcaatcg ctatggctgt tttgcaaaaa
     4501 ccgaaactta ttctatgtga tgagatcagc tctgccttag atactaatag tgaaaaagag
     4561 atatttaact tgttgtctga tcttcgtgaa aaatatggta tctctatctt gatgattgct
     4621 catcacctat ctcttttaaa acaatattgt gatcgtgtaa tgattttgga tcaccagact
     4681 attgtagata ctgttgttcc agtgaaagca actctgaatc aattaaagtc aaactacgta
     4741 gatcaagtaa aggagttttt gttacatgat tgacattatt gaaaaacatg gcgaaggtat
     4801 cttaaaatca ttttgggaaa ccaatttaat gttagggtta accttgattc tttgttttct
     4861 aattgctttt ccaacaggta ttttattgtt ttcattgaga aagtcttact tgattaaaca
     4921 ttctttagcc tatcaactac taaatttatt tttaggtacg ttacggagtg tccctttcct
     4981 tatttttatt tttatcttaa tccctctcaa tcgattaatt tttgggacct catttggaac
     5041 gatagcggca atcttacctc ttactttggt ttcagtcagt ttgtatgcac gttatgtgga
     5101 acaagcttta ttaaatatac ctcaagttgt tgtagatcga gctctcagtc taggggctaa
     5161 caagagacag ataatttatt attttttaat cccctccatc aagattgacc ttgttttatc
     5221 atttactgca acggctataa gtattcttgg ctactcgaca ataatgggag ttattggtgc
     5281 aggaggtttg ggtgaatatg cctatcgttt tggctatcaa gagtatgact acccagttat
     5341 gtacttgatt gttgtcttat ttataatata tgtgtttatc ttacagagtt tgggctattt
     5401 tatagcaaac cgctactcaa gaaaataaaa gaaaagagga actatgaaaa aattactttc
     5461 cctaacatgt ctaatcatga tgtctttatg tttagtggca tgtactaagc aagcaatgtc
     5521 gtctaagcaa attaaagata agaatagtaa agaaaaggtg attactgttg caacttacag
     5581 caaacctaca tctacctttt tagatttgat taaagataat gtaaaagaaa aaggatatac
     5641 tttaaaggtt gtcatggtct ctgactatat tcaggctaac attgctttag aaaacaaaga
     5701 acatgatgct aaccttttac aacatgaatt tttcatgagt atctttaata aggaaaatga
     5761 tggtcatcta gtgtcaatta caccaattta tcattcattg gctggttttt atggtcaaca
     5821 tttgaaaaat attgccgagc ttaaagacgg tgctaaggta gcgattccgt ctgatcctgc
     5881 caatatgact agagctctgc tattattgca agaaaagaaa cttatcacct taaagaatac
     5941 gtccaaaaag accaaggcta tcgaagatat tattactaac cctaaaaaat tacgaattga
     6001 acctgtagca ttacttaacc tcaatcaggc ctattttgaa tatgaccttg tctttaattt
     6061 ccctggatat gtgacaaaaa tcaatctagt tcctaaaagg gatagattat tatatgagaa
     6121 aaaaccagat atccgttttg caggtgcctt ggtagctcgt gaagataata aaaatagtga
     6181 taaaataaaa gtacttaaag aagtactaac aagtaaagag attcgtcact atatcactaa
     6241 ggagattcca agtgaagcag acgttgcgtt ctagatagat aaactatgaa ccctccttct
     6301 ttatagatgg agggttttta ttgccttttt tctattttct gaagcaacta atagaactag
     6361 caatttatga aagcgtgtta tttattagat tgaccttatt ttatatcttt ttacttttaa
     6421 ttgtgttaca ctaagtaggt tgtcagaaaa gataactaat aaaggttcat aaggcagctt
     6481 cgcactgtct tttagaaaag agaaacattt gaaatttaca gaacttaact tatcgcaaga
     6541 tattctttca gcggtagaaa aagcaggctt tgtagagcca tcaccaatcc aagaaatgac
     6601 tatcccactt gctcttgaag gcaaagatgt cattggtcaa gcgcagacag gtacaggaaa
     6661 aactgcagct tttgggttac caactttaaa taaaattcat actgaagata atactattca
     6721 agcacttatt attgctccta cacgtgaact tgcagtacaa tcacaagaag aattgttccg
     6781 ttttggacgc gataaaggtg ttaaggtccg ctctgtttat ggtgggtcaa gtattgaaaa
     6841 acaaatcaag gcattacgtt caggagcaca cgttgttgtt ggaactccag gtcgattact
     6901 tgatttaatt aaacgtaaag cacttaaact taatcacatc gaaactctta ttcttgatga
     6961 agcagacgaa atgcttaaca tgggattttt agaggacatt gaagcaatca ttagtcgtgt
     7021 accagaagca cgtcaaactc ttttattctc agcgacaatg cctgatccaa tcaaacgtat
     7081 cggggttaaa ttcatgaaag atcctgagca tgttaaaatt aaagcaactg aattaactaa
     7141 tgtgaatgtt gatcagtatt atgttcgcgt taaggaaaat gaaaaattcg atactatgac
     7201 acgtttgatg gacgttgatc aaccagaatt gtcaattgtc tttggacgta cgaaacgtcg
     7261 tgtagatgaa ttaactcgtg gtctaaaatt acgtggtttc cgagcagaag gaattcatgg
     7321 agacttagat caaaataaac gtcttcgtgt tatcagagac tttaaaaatg accatattga
     7381 tattcttgtt gccacagatg ttgctgcgcg tggtttagat atttcaggcg ttacacatgt
     7441 ttacaactat gatattcccc aagatccaga aagttatgtt caccgtattg gacgtactgg
     7501 tcgtgctggt aaatctggtc aatctatcac ttttgtttca ccaaacgaaa tgggatattt
     7561 gacaattatt gaaaacttaa ctaaaaaacg tatgactgga atgaagcctg ctactgcaag
     7621 cgaagcattt caggctaaga aaaaagttgc attgaaacgt attgcacgtg actttgagga
     7681 tcaagaatta gtttctaaat ttgataaatt taaagccgat gctttagaat tagcaactca
     7741 atatacacca gaagaacttg ctctttatgt cttaagctta acagttcagg atccagaatc
     7801 gttaccagaa gttgaaatca cacgtgaaaa accattacca tttaaaccat caggtggtgg
     7861 ctttaaaggt aaaggtggac gaggtaatgg ccgtggtggc gatcgtcgac gcaatgatcg
     7921 tggtgatcgt cgtggaaatc gtgaccgtga tgatcgtgga ggtcgtcgtg attttaaacg
     7981 tcgtgatgat aagttcaaga aagataatcg tcgtcaagaa aataaaaaac ctcataaaaa
     8041 cacttcaagt gaaaaacaaa ctggatttgt tattcgcaac aaaggcgata aataagagga
     8101 ttacgaaaaa ggagttgctg agcgactcct tttttatttt ttaagataaa gcgtagatag
     8161 tgatatccgt aaaattgcct tataacttta agaatgaaga caaaagatta aagtgaggtt
     8221 gaaatttaaa agttaaagaa gcgaggacag gctcattgca cttagtatat tatcgtatta
     8281 ttgttcattt ttatgttgtt taatataagt caattttgct ttccgagtct tttgtttaaa
     8341 taatgcttct gccctcattg cttcttgttt gctattaaaa gcttcagaat aaagaagttt
     8401 tactggcaat ctcgcacgag tatatttagc accttttccg gtattgtgtg tattgagacg
     8461 gcgcttgaca tctgtagtat aacctgtata aagtgttcca tctgaacatt ctaaaacata
     8521 catataagca ggaactttaa tcatgagatt ttccgttttc cccgaaatat atatcaaaaa
     8581 tagtctcagt atagtctcca ttatcttgat gaacaacaag aggaggaagt attttcatac
     8641 cttctgtcga gccatcttta atagcttcaa ttaaaagcat attagcatcc tttcctaatt
     8701 ttggataaac aaattggata cgtttaggtg ctaaattgaa ctgacgcatc gtatcaataa
     8761 tttcaaggaa acggtctgga cgatgtacca tagcgatacg tccttttgtt tttagagcat
     8821 gttgagcaat ttgacaaatc tctctaaggt ttgttgttat ttcatgtctt gcaaggagat
     8881 aatgcgggct tagatttttt ttagaggttt cgcttgcttt gaaataaggt ggattgcaaa
     8941 gcattaaatc aacgtttgaa cgttgaacat gatcaagaag attttttaaa tcatcattaa
     9001 tcatagtaac ctgtttttcc aatttattga gcttaattga acgcttagcc atatccgcaa
     9061 ggcgctcttg tagttcgatc tctataattg tcgcacttgt tttggtgctg gcaaacaaac
     9121 cgacagcacc attaccactg cataagtcaa cgataagacc acgagaagga agtttaggga
     9181 accgagacaa taaaacacta tctatggagt aggaaaaaac ttctttgttt tggatgattt
     9241 tgacatcagt tgaaaaaagc tgatcaatgc gttcattttc ttttaaaatt gtatctatca
     9301 taggcttatt atagcaaaat tacagcttcc tagcactagt tttgaaagtg tggaggttga
     9361 ttttaagata attctttcta aaaaaaagga agcatgataa aatagaagtt acttgatgaa
     9421 aggtagagga gaaacatgtt ctatacttac ttacgtactt tagttatgtt cttgatttgg
     9481 gtggctaatg gtaatgcgca ctatcataat gaagataaaa tgttaaaaga tgatgaaaat
     9541 tatattttag tagctccaca cagaacattt tgggatcctg tgtacatggc ttttgctgct
     9601 agacccaaac agtttgtttt tatggccaaa aaagaattat tcacgaatcg tttgtttggt
     9661 tggtggatta aaatgtgcgg tgctttccct attgatcgtg aaaagccagg gcaagacgct
     9721 attcgatatc ctgtaaagat gctgaaaaat agtaaccgtt ctttggtgat gtttccgagt
     9781 ggaagccgcc actccaaaga tgtcaaagga ggcgtagctg ttattgcaaa aatggcaaaa
     9841 gtcagaatta tgccagcagc atatagaggt cctatggttt ttaaaaatct attaaagggg
     9901 catcgtgttg atatgaattt tggtaaccct attgatgttt ctgatattaa gcgtatggat
     9961 gccgaaggga ttgcagaagt atctcgtcgt attcaggaag aatttgatcg tttagataga
    10021 gaaaatgaaa cttatgatga tggtaagaaa ttaaatccat tgacttatat ttatcgtcta
    10081 cctttagcaa ttatagctat tgtcttgctt gtcctaactc taatttttag ttatttagca
    10141 agctttgtgt gggatccaca aaaacatcta aaataagggt tacatggaaa ctatttactt
    10201 tttagtaaat agtttttttt gtttttaat
//
LOCUS       DATROY010000068        10162 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.069, whole genome shotgun sequence.
ACCESSION   DATROY010000068 DATROY010000000
VERSION     DATROY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            11..4093
                     /gene="cas9"
                     /locus_tag="VBD11_001304"
     CDS             11..4093
                     /gene="cas9"
                     /locus_tag="VBD11_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286032.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6954254.1"
                     /translation="MGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIKKNLIGALLFD
                     GGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLEDSFLVEDDKR
                     GSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAHIIKFRGHFLI
                     EDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKISKSAKKDRILA
                     QYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDLENLLGQIGDE
                     FADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLKQLKQFVKASL
                     PEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLEKIKNEDFLRK
                     QRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIPYYVGPLAREK
                     SDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKVLPKHSLIYEK
                     FTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLDFLAKEYEEFR
                     IVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQTLTLFEDREMI
                     KKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTILDYLISDGRAN
                     RNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGILQSLKIVDELV
                     KVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNGNILKEYPTDN
                     QALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDSIDNRVLVSSA
                     KNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLTSDDKARFIQR
                     QLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFRKEFGFYKIRE
                     VNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSATEKLFFYSNI
                     MNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYPQVNIVKKTEI
                     QTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLVVADIKKGKAQ
                     KLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSLFELENGRRRL
                     LASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQHVSYFDDILQ
                     IINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSLGAPAAFKFFD
                     KIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            4095..4964
                     /gene="cas1"
                     /locus_tag="VBD11_001305"
     CDS             4095..4964
                     /gene="cas1"
                     /locus_tag="VBD11_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6954255.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            4961..5302
                     /gene="cas2"
                     /locus_tag="VBD11_001306"
     CDS             4961..5302
                     /gene="cas2"
                     /locus_tag="VBD11_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6954256.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            5289..5954
                     /gene="csn2"
                     /locus_tag="VBD11_001307"
     CDS             5289..5954
                     /gene="csn2"
                     /locus_tag="VBD11_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6954257.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   6055..7080
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6055..6090
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            7637..8053
                     /gene="ndk"
                     /locus_tag="VBD11_001308"
     CDS             7637..8053
                     /gene="ndk"
                     /locus_tag="VBD11_001308"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6954258.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            8189..10021
                     /gene="lepA"
                     /locus_tag="VBD11_001309"
     CDS             8189..10021
                     /gene="lepA"
                     /locus_tag="VBD11_001309"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6954259.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
BASE COUNT         3347 a         1545 c         2049 g         3221 t
ORIGIN      
        1 aggtctcgac atcggaacta attccgtcgg atattctgtg gttacagatg attataaggt
       61 acctgctaag aagatgaggg tcttagggaa tacagataaa gaatacataa agaagaatct
      121 tataggtgct ctgctttttg atggtggaaa tactgcttca gatagacgct tgaagcgaac
      181 tgctcgccgt cgttatacac gtcgtagaaa tcgtattcta tatttacaag aaatttttgc
      241 agaggaaatg agtaaagttg atgatagttt ctttcatcga ttagaggatt cttttctagt
      301 tgaggacgac aagagaggga gcaagtatcc tatctttgca acaatgcagg aggagaaaga
      361 ttatcatgaa aagttcccaa cgatttatca cttgagaaaa gaattagctg acaagaaaga
      421 aaaagcagac cttcgtcttg tttatctggc tctagctcat atcattaaat tcagaggaca
      481 tttcctaatt gaggatgata gctttgatgt gaggaatact gatattcaaa gacaatatca
      541 agctttttta gaaatctttg atactacctt cgaaaataac catttgttat ctcaaaacat
      601 agatgtagag ggaattctaa cagataagat tagcaagtct gcgaagaaag atcgcatttt
      661 agcgcagtat cctaaccaaa aatctactgg catttttgca gaatttttga aattgattgt
      721 cggaaatcaa gctgacttca agaaacattt caatttggaa gataaaacgc cgcttcaatt
      781 tgctaaggat agctacgatg aagatttaga aaatcttctt gggcagattg gtgatgaatt
      841 tgctgactta ttctcagtag cgaaaaagct atatgatagt gttcttttat ctggcattct
      901 tacagtaact gatctcagta ccaaggcgcc actttctgcc tctatgattc agcgttacga
      961 tgaacataga gaggacttga aacagttaaa acaattcgta aaagcttcat tgcctgagaa
     1021 atatcaagaa atatttactg attcatcaaa agatggctac gctggttata ttgaaggcaa
     1081 aactaatcaa ggagcatttt ataaatactt gtcaaaattg ttgaccaagc aagaaggtag
     1141 cgagtatttt cttgaaaaaa tcaagaatga agattttttg agaaaacaaa ggacctttga
     1201 taatggctca attccacacc aagtccattt gacagaattg aaggctatta ttcgacgtca
     1261 atcagaatac tatccattct tgaaagagaa tctagatagg attgaaaaaa tccttacctt
     1321 tagaattcct tattatgtcg ggccactagc acgtgagaag agtgattttg catggatgac
     1381 tcgcaaaaca gatgacagta ttcgaccttg gaattttgaa gaattggttg ataaggaagc
     1441 atctgcggaa gcttttatcc atcgtatgac caacaatgac ctctatcttc ctgaagaaaa
     1501 agtcctacca aaacatagtc ttatttatga aaaatttacg gtctataatg agttgactaa
     1561 ggttagatat aaaaatgagc aaggtgagac ttattttttt gatagcaata ttaaacaaga
     1621 aatctttgat ggagtattca aggaacatcg taaggtatcc aagaagaagt tgctagattt
     1681 tctggctaaa gaatatgagg agtttaggat agtagatgtt attggtctag ataaagaaaa
     1741 taaagctttc aacgcctcat tgggaactta ccacgatctc aaaaaaatac tagacaaaga
     1801 ttttcttgat aatccagata atgagtctat tttggaagat atcgtccaaa ctctaacctt
     1861 atttgaagat agagaaatga ttaagaagcg tcttgaaaac tataaagatc tttttacaga
     1921 gtcacaacta aaaaaactct atcgccgtca ctatactggc tggggacgat tgtctgctaa
     1981 gttaattaat ggcattagag ataaagagag tcaaaaaaca atcttggact atctgatttc
     2041 tgatggaaga gcaaatcgca actttatgca gttgatacat gatgatggtc tatcatttaa
     2101 accaattatt gacaaggcac aggctggtag tcattcagat aatctaaaag aagttgtagg
     2161 tgagcttgca ggtagccctg ctattaaaaa ggggattcta caaagtttga aaattgttga
     2221 tgagcttgtt aaagtcatgg gatacgaacc tgaacaagtt gtggttgaga tggcgcgtga
     2281 gaatcaaaca acaaatcaag gtcgtcgtaa cactcgacaa cgctataaac ttcttgagga
     2341 aggcgttaag aatctagcta gtgacttgaa tggcaatatt ttgaaagaat atcctacgga
     2401 taatcaagcg ttgcaaaatg aaagactttt cctttattac ttacaaaatg gaagagatat
     2461 gtacacaggg gaagcactag atattgacaa tttaagtcaa tatgatattg atcacattgt
     2521 tcctcaagct ttcataaaag atgattctat tgataatcgt gttttggtat catctgctaa
     2581 aaaccgtgga aaatcagata atgttcctag cattgatatt gtaaaagcac gcaaagcatt
     2641 ctggaaaaaa ttacttgatg ctaagttaat gagtcagcgt aagtatgata atttgactaa
     2701 ggcagagcgc ggaggcctaa cttccgatga taaggcaaga tttatccaac gtcagttggt
     2761 tgagacacga caaattacca agcatgttgc ccgtatcttg gatgaacgct ttaataatga
     2821 gcttgatagt aaaggtagaa ggattcgcaa agttaaaatt gtaaccttga agtcaaattt
     2881 ggtttcaaat ttccgaaaag aatttggatt ctataaaatt cgtgaagtta atgattatca
     2941 ccatgcacat gatgcctatc ttaatgcagt agttgctaaa gctattctaa ccaaatatcc
     3001 tcagttagag ccagaatttg tctacggtga ctatccaaaa tataacagtt acaaaacgcg
     3061 taaatccgct acagaaaagc tatttttcta ttcaaatatt atgaacttct ttaaaactaa
     3121 ggtaactttg gcggatggaa ccgttgttgt aaaagatgat attgaagtta ataatgatac
     3181 gggtgaaatt gtttgggata agaagaaaca ctttgcgaca gttagaaaag tcttgtcata
     3241 ccctcaggtc aatatcgtga agaagacaga gattcagaca ggtggtttct ctaaggaatc
     3301 aatcttggcg catggtaact cagataagtt gattccaaga aaaacgaagg atatttattt
     3361 agatcctaag aaatatggag gttttgatag tccgatagta gcttactctg ttttagttgt
     3421 agctgatatc aaaaaaggta aagcacaaaa actaaaaaca gttacggaac ttttaggaat
     3481 taccatcatg gagagattca gatttgagaa aaatccatca gctttccttg aatcaaaagg
     3541 ttatttaaat attagggacg ataaattaat gattttaccg aagtatagtc tgttcgaatt
     3601 agaaaatggg cgtcgtcgat tacttgctag tgctggtgaa ttacaaaaag gtaacgagct
     3661 agccttacca acacaatata tgaagttctt ataccttgca agtcgttaca atgagctaaa
     3721 aggaaaacca gaagaaattg aacaaaaaca ggaatttgta gttcaacatg tttcttattt
     3781 cgacgatatt cttcaaataa tcaacgattt ttcaaatcga gttattctag cagatgctaa
     3841 tttagagaaa atcaataagc tttaccaaga taataaggaa aatatatcag tagatgaact
     3901 tgctaataat attatcaatc tatttacttt taccagtcta ggagctccag cagcttttaa
     3961 attttttgat aaaatagttg atagaaaacg ctatacatca actaaagaag tacttaattc
     4021 taccctaatt catcaatcta ttactggact ttatgaaaca cgtattgatt tgggtaagtt
     4081 aggagaagat tgatatggca ggttggcgaa ccgttgttgt aaatacacat tctaagctct
     4141 cttataaaaa taatcatctg gtttttaaag attcttatca gacggaaatg attcatctat
     4201 cagagattga cattctaatc atggaaacaa cagatatcgt tttgtcgacc atgctgatta
     4261 aacgtttggt tgatgaaaat attttagtta tattttgtga tgacaaacgc ttgccaacag
     4321 ctatgttaat gccgtactat gccagatatg attcgagttt acaattatct aggcagatgt
     4381 catggattga agatgtcaaa gcggatgttt ggacgtcaat cattgcacaa aaaattttga
     4441 atcagtcttt ttatcttggt gagtgttctt tctttgaaaa atcccagtct attatgaatc
     4501 tctatcatga tttagaatct tttgatactt ccaatcgtga agggcatgca gctaggattt
     4561 atttcaatac actttttgga aatgattttt caagagaaca tgataatcca ataaatgctg
     4621 gtttagacta cggatattca ttgcttttga gtatgtttgc gcgtgaagtc gttaagtgtg
     4681 gttgcatgac acaatttggg ttaaagcatg ctaatcaatt taatcagttc aacctagcaa
     4741 gcgatattat ggaaccattt cgcccaatcg ttgataggat tatttatgaa aataggcaga
     4801 gtgattttgt caaaatgaaa agagaactct tttctatgtt ttcagagaca tacagttacg
     4861 acggtaaaga aatgtatctt tcaaatattg tcagcgatta caccaaaaaa gttattaagt
     4921 cgctaaatag tgatgggaat gggattccgg agtttaggat atgagttatc ggtatatgag
     4981 aatgatttta atgtttgata tgcctactga aacagtagaa gaacgtaagg cgtatcgtaa
     5041 gtttagaaag tttcttttga gcgaaggttt tattatgcat cagttctctg tttatagtaa
     5101 attattactc aataatacag ctaataatgc tatgataggt cggcttaaag tgaataatcc
     5161 taagaagggt aatatcacac tcttaacagt tacggaaaaa caatttgcga gaatggttta
     5221 cctccatgga gaacgcaaca caagtgttgc caactttgat agtcgcttgg ttttcctagg
     5281 agattcttat gatcaagatt aattttccaa ttttagatga accattagtg ttaagtaatg
     5341 ctacgatttt aacgatagaa gatgtttcag tttattcttc attggtgaaa catttttatc
     5401 aatatgacgt agatgaacat ttgaaattat ttgatgataa gcagaaaagt ctgaaggcaa
     5461 cagagttaat gatggttaca gatatcttag gatacgatgt caactcagca cctattctaa
     5521 agttgataca tggtgactta gaaaatcaat tcaacgaaaa gccagaagtg aaatcaatgg
     5581 tagaaaaatt agcagctact attacagaac ttatcgcatt tgagtgtcta gagaatgagc
     5641 ttgatttaga atacgatgaa atcacgattt tagaactcat taaggcacta ggagtcaaaa
     5701 ttgagacaca gagcgacact atctttgaaa aatgttttga aattatacaa gtttaccatt
     5761 atttaacgaa aaagaatctc ttggtttttg ttaatagcgg agcttatctt accaaagatg
     5821 aagttataaa attatgtgaa tacatcaatt taatgcaaaa gtcagtactc tttctagaac
     5881 ctagaagact ctatgattta ccgcaatatg ttattgataa ggattatttc ttgataggcg
     5941 aaaatatggt ataatattag taaaagcaca gtaataacaa ggaatcatcg aaactgaagt
     6001 cctgctgaga cgaatggcgc gattacgaaa gctcaaaaga aaattttcta cgaggtttta
     6061 gagctgtgct gtttcgaatg gttccaaaac aacgttgtgt aggctctttt gatagcgttt
     6121 gttttagagc tgtgctgttt cgaatggttc caaaactaaa tatgacttta acgcaccttc
     6181 ctctatgttt tagagctgtg ctgtttcgaa tggttccaaa acaaaggata gtgggtctat
     6241 tcttatcttg tggttttaga gctgtgctgt ttcgaatggt tccaaaacac gagctaacgg
     6301 ttacctaatc agtcgccggt tttagagctg tgctgtttcg aatggttcca aaactatcga
     6361 tattgatgat ttaacatcaa ttatgtttta gagctgtgct gtttcgaatg gttccaaaac
     6421 tgctaaaagg taaatttaac attccaggta gttttagagc tgtgctgttt cgaatggttc
     6481 caaaacagag tgtgtccaag accagagtta ctgtttgttt tagagctgtg ctgtttcgaa
     6541 tggttccaaa acctattggt tagaattttt ttacaggaag aagttttaga gctgtgctgt
     6601 ttcgaatggt tccaaaactg ctaaaaggta aatttaacat tccaggtagt tttagagctg
     6661 tgctgtttcg aatggttcca aaactgtttt aatcatccaa aatatgttgc ttaagtttta
     6721 gagctgtgct gtttcgaatg gttccaaaac tcatctggtg taacttgttc ggccatcatc
     6781 gttttagagc tgtgctgttt cgaatggttc caaaactcta aaatcaagga actgagaaaa
     6841 aataaagttt tagagctgtg ctgtttcgaa tggttccaaa actggtggta tttataatgt
     6901 acgagcaaat cggttttaga gctgtgctgt ttcgaatggt tccaaaacga taaaaagtgg
     6961 gagctgaatt aaaaggcagt tttagagctg tgctgtttcg aatggttcca aaacatttga
     7021 acgattttta tattcctgat atgtgtttta gagctgtgcg gttattatgc tagggcaccg
     7081 ttgtggtgtt ctagtttttt gttatactga aataaatttt cagagaatgt gggggaaggc
     7141 ggtaattaga ttaattcaag acgtaattca gaacttagtt ggccaagcta acgaaatcac
     7201 cccagtttat cagtttgatt gggaaactta tatattggcg actaaaaaat atgaacgtca
     7261 tttagaggtg tgtctattag tagaaaattc gaattatttt tcggattcaa agagaatgtg
     7321 tcgatagaag agatacgaaa gactataatt ccaaccttat ggttaaaggg ctagattgtt
     7381 ctacgcttta cttaattatt agtttgacag cgttggttct tttagtgatt gctggtgttt
     7441 ctttgtcact aatacttgca agcttacaag gaaagcagtg gagaacctat ccataatcaa
     7501 ctaacagcta tgatggatct cacctattaa tatagttaag gagtgtccga cactcctttt
     7561 ttgaaataca gttggaagtt acagaaaaca tattttcatg ttataatagt ggagaaagaa
     7621 atgaggtgct aatattatgg aacaaacctt ttttatgata aaacctgatg gtgtaaaacg
     7681 tggttttatt ggagaagtta ttagtcgtat tgagcgtcga ggcttctcta ttgatcgctt
     7741 agaggttaga catgcagatg cagatatttt gaaaagacat tatgcagaac tgacggatag
     7801 accattcttt ccaacattag tagattatat gactagtggt ccagtaatca ttggagttat
     7861 ttcgggagaa gaggtcattt caacttggcg caccatgatg gggagtacta accccaaaga
     7921 tgcccttcca ggaactattc gaggagattt tgcacaagct ccctctccaa accaagcgac
     7981 atgtaatatt gttcatgggt cagactcacc tgaatctgca gctcgcgaga tagccatttg
     8041 gttcaataac taatagactt aatgaagatt gatgttcaag tgttaattct cttttacatc
     8101 ggtcttcttt ttgatataat attctagagt ctataggact gttttaatat tttagaagtt
     8161 aaacatatca aattgtatag gaaatagaat gaatattgaa gatttgaaga aacgtcaaga
     8221 aaagattaga aacttttcga ttatcgctca tattgaccat ggaaaatcaa ccttagcaga
     8281 ccgtatttta gagaaaacag aaacggtttc tagtcgtgaa atgcaggcgc aactgttgga
     8341 ctctatggac ctagaacgtg agcgtgggat tactataaag ttaaatgcta ttgagcttaa
     8401 ttatactgct aaagatggtg aaacttacat ttttcacttg attgacacgc cagggcatgt
     8461 tgactttaca tatgaagtat cacgttcatt ggcagcttgt gagggtgcta tactggttgt
     8521 tgatgcagcc caaggaattg aagctcaaac tctggcaaat gtttatttgg ctctagacaa
     8581 tgatttagag atactaccag ttattaataa aattgattta ccagcagcag atccagaacg
     8641 tgtacgtgca gaagtagagg atgttattgg tctagatgct tcagaagctg tacttgcctc
     8701 agctaaggca gggattggta ttgaagaaat tttagagcag atcgttgaaa aagttcctgc
     8761 tccgactggg gaagtagatg ctcctcttca agctctaatt tttgattctg tttacgatgc
     8821 ttatcgtgga gtaattctgc aagtaaggat tgtaaatggt atggttaagc caggagataa
     8881 gattcaaatg atgtctaatg gtaaaacctt cgacgttact gaggttggta ttttcactcc
     8941 caaagcagtt ggacgtgatt ttttagcaac tggagatgta gggtatattg ctgcctcaat
     9001 taaaacagtt gctgatacac gtgttggtga taccattacc cttgctaata acccagctat
     9061 agaccccttg catggctata agcaaatgaa cccaatggtt tttgccggtc tttatccaat
     9121 tgaatcaaat aagtataacg atctgcgtga agcccttgaa aaattacaat tgaatgatgc
     9181 tagtctacag tttgaaccgg aaacatcaca agcattaggg tttggtttcc gttgcggttt
     9241 cttagggctt ttacatatgg acgttatcca agagcgcttg gaacgagagt tcaatattga
     9301 tcttattatg actgcaccat cagtagttta tcatgttaat acaactgatg gtgaaatgct
     9361 tgaagtttcg aatccatctg aatttcctga tcctactcgt gttgacagca ttgaagaacc
     9421 atatgttaag gcgcaaatta tggttcctca agaatttgtc ggtgctgtaa tggagttagc
     9481 acaacgtaaa cgtggtgact ttgtaacaat ggattatatt gatgataatc gtgttaatgt
     9541 tatttaccaa attcctttag ctgaaattgt gtttgacttc tttgataagc ttaaatcatc
     9601 aacacgtggc tatgctagtt ttgattatga aatttccgaa tatcgtcgct cacagttagt
     9661 taaaatggat attcttttaa atggtgataa agtggatgct ttaagcttta ttgttcacaa
     9721 agaatttgcc tatgaacgtg gtaaattgat cgttgataag ttgaaaaaaa tcattcctcg
     9781 tcagcagttt gaagttccta tccaagcagc tattggacaa aaaattgtgg ctcgttctga
     9841 tataaaagca ctacgcaaaa atgtcttagc aaaatgttat ggtggtgacg tttcacgtaa
     9901 acgtaagttg cttgaaaaac aaaaagctgg taaaaagcgt atgaaagcaa ttggttccgt
     9961 cgaagttcct caggaagctt tcttaagtgt tttatcaatg gacgatgatg acaaaaaata
    10021 attcagaaag atgatatcat ttatctattt taaagtaact tagcttcgtg cttaagttac
    10081 tttttctttg tctgcaatta tcaaataata tggctttata atatctttat ttaaatataa
    10141 atgagctata aatgagtttt tg
//
LOCUS       DATROY010000069        10041 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.070, whole genome shotgun sequence.
ACCESSION   DATROY010000069 DATROY010000000
VERSION     DATROY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1061
                     /locus_tag="VBD11_001310"
     CDS             <1..1061
                     /locus_tag="VBD11_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6954260.1"
                     /translation="RKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTKEN
                     GTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFYYN
                     DGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYEKQ
                     LKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQDQF
                     GISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKAAA
                     ETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRWHR
                     PNGQYASKAEIAAAGLQW"
     gene            complement(1169..2476)
                     /gene="eno"
                     /locus_tag="VBD11_001311"
     CDS             complement(1169..2476)
                     /gene="eno"
                     /locus_tag="VBD11_001311"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6954261.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            2674..3129
                     /locus_tag="VBD11_001312"
     CDS             2674..3129
                     /locus_tag="VBD11_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6954262.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            3142..3627
                     /locus_tag="VBD11_001313"
     CDS             3142..3627
                     /locus_tag="VBD11_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6954263.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(3648..4289)
                     /gene="serB"
                     /locus_tag="VBD11_001314"
     CDS             complement(3648..4289)
                     /gene="serB"
                     /locus_tag="VBD11_001314"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6954264.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(4383..6107)
                     /gene="ezrA"
                     /locus_tag="VBD11_001315"
     CDS             complement(4383..6107)
                     /gene="ezrA"
                     /locus_tag="VBD11_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6954265.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KAQERIRF"
     gene            complement(6201..8153)
                     /gene="gyrB"
                     /locus_tag="VBD11_001316"
     CDS             complement(6201..8153)
                     /gene="gyrB"
                     /locus_tag="VBD11_001316"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6954266.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(8154..8714)
                     /locus_tag="VBD11_001317"
     CDS             complement(8154..8714)
                     /locus_tag="VBD11_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6954267.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(8833..10038)
                     /locus_tag="VBD11_001318"
     CDS             complement(8833..10038)
                     /locus_tag="VBD11_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6954268.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
BASE COUNT         3264 a         2014 c         1599 g         3164 t
ORIGIN      
        1 aaagaaaaca gtttataaaa ttaggaattg caaccttact aacggttatt tcgctttaca
       61 caccaataaa cctagctaca aatcatacca cagaaaatat tgttactgct caagagtata
      121 aaacaaaaga gaatggtact ttacctttta aacacaaacg acaattggtt ttaggagagt
      181 tggatgacaa aggtcgtgcc acattcgcac atattcagct aaaagttaaa gatgaaccca
      241 agaaaaagag agtaaaacgt ttaaaaacaa cccctgttgg atggcacaat tttaaattct
      301 attataatga tggtactcaa aaagcttggc taatgagccg aggccgtctt atttgccacc
      361 aatttagtgg actaaacaat gaaagaaaga atttagttcc aatgacgaat tggctaaata
      421 ctggaaacta caatagcacg aattcatcta accctgagag catgctattc tatgaaaaac
      481 aactaaagac ttggctttcg actcataaaa actactactt agattataaa gtaacaccaa
      541 tttatcaaaa taatgaacta attccaagaa aaattgaatt aaaatatgtt ggtattgata
      601 agactgggaa attattacct atttttatcg gaaacaaatc cactcaagat caattcggca
      661 tcagcactgt taccttagag aatacctcac caaatgctac cattgactac ctcagcggaa
      721 aggctcaaaa tactgtctta tcagctaaag aacaaagaaa acttatcgct aagcacgaag
      781 aagaaaaacg acttgcagaa aagaaagttg aagaggaaaa agctgctgct gaaactcaga
      841 aaaaactgga agaagaacaa gcaaggcttg ctgctgaagc tcaaaggaag caaaaagagg
      901 agcaagcaag gctcgcagct gaaactcaga aaaaacaaga aactctagta caagaacaaa
      961 ctagtcaagg ctacaaacgt gactatagag gaaggtggca tcgtccaaat ggacaatacg
     1021 cttcaaaagc agaaatcgct gctgctggat tgcaatggta gaactgttta ttatcaaatc
     1081 aattctatta ttataatgtg tacataaaaa ccatcttgca tatgcaagat ggtttttatt
     1141 atattttcaa ctgtttaata atctgagact atttttttaa gttgtagaat gatttgatac
     1201 ctttgtattg agcaacttca ccaagttggt cttcgatacg aagtaattgg ttgtatttag
     1261 caatacggtc agtacgtgat aatgaacctg ttttaatttg accagcgtta gttgcaactg
     1321 cgatatcagc gattgttgaa tcttcagttt cacctgaacg gtgtgataca actgcagtgt
     1381 aaccagcttc tttagccatt tcaatagctt cgaaagtttc tgtcaaagta ccgatttggt
     1441 taactttgat aaggattgag ttagcagctt cttctttgat accacgtgct aagtaatcag
     1501 tatttgtaac gaagaaatcg tcaccaacta attgtacacg tccaccaagg cgttctgtaa
     1561 gagctttcca accatcccag tcgttttcat ccataccatc ttcaatagtg atgattgggt
     1621 atttgttaac caactcttca aggtaatcga tttgttctgc agcagtacga actgctccac
     1681 cttcaccttc gaatttactg tagtcgtata ctttacgttc tgcatcgtag aactctgatg
     1741 aagcacagtc aaaaccaatc atgattccgt tttcgccagc ttcgtaacca gctgcttcaa
     1801 tagctttaag aatagtttct acgccatctt cagtaccttc aaatttaggt gcaaatccac
     1861 cttcatcacc aacagctgtt tcaagaccac gttctttaag gattttctta agagcatgga
     1921 atacttcagc accccaacga agtgcttctt tgaaagttgg tgcaccaact ggcataatca
     1981 tgaattcttg gaatgcgatt ggagcgtctg agtgagaacc accgttgatg atgttcatca
     2041 ttggagttgg aagaacttta gtgttgaatc caccaaggta gctgtaaagt ggtacttcaa
     2101 ggtaatcagc tgcagcacgt gctacggcaa tagaaacacc aagaatagca tttgcaccta
     2161 atttaccttt attaggagtt ccgtcaagag cgatcatagc acggtcgata gcttgttggt
     2221 cgcgtacatc ataaccaatg attgcttctg caataacgtt atttacattg tcaacagctt
     2281 tttgtgttcc aagaccaccg taacgagact tgtcgccgtc gcgaagttca actgcttcgt
     2341 gttcaccagt agaagctcct gaaggaacca taccacgtcc gaaagcacct gattctgtat
     2401 aaacttctac ttcaagtgtt gggttaccgc gtgagtcaag gacttcgcgt gcgtaaacat
     2461 cagtaataat tgacattaca atactctcct tatgagtttt aattttttac atattcataa
     2521 tatcataatc tttagttaat aacaaggaaa aacccagtga aaccgcttta ttacgtggtt
     2581 tttgataaaa aaatcactaa ttcttttcaa gtttacctgt gctaatgcaa cccatttagg
     2641 ttataataaa attagcaaat tcaaggagaa attatgactg aacttgacaa taaaatccta
     2701 caaaaagcaa gcggtgaaaa tcgtcttgat cctgatcaac aacgttacta ccttggtact
     2761 tataaagaaa gagttgtgct ccacttaagt ttaaaagagg cacaagaaga aaagtttcaa
     2821 cttgctataa catctgctct taataatctg atacttgaat atcctaatct tagtttaaaa
     2881 ttatcaccaa atctccctac tgatgttcaa atgttctaca tgaagttagc taaccgtcta
     2941 tcactagctg ctacgattgt taatgaagca aatgccaatt ctccatttgg tattattata
     3001 catagtgata aggctgaaaa cgttgaatgg aacgattttg agacacaatt ccctgattta
     3061 ttcaattcac caaaaaaaga ggagtctcct aaaaaaagtc tatggcaaca tttctttagt
     3121 caaaaataac aaggaggtct aatggaaatt tgggatgctt atgaccaata tgggttcatt
     3181 acaggtctta cacttaatag ggatcaaaat atcccacaag gcttattcca cttagttgtt
     3241 gatgttatcc tttttcatga ggatggtgac gtattgatga tgaaacggca ccctaaaaag
     3301 aaagcttttc ctgcttattt cgaagccacc gctggcggat ctgcccttaa aggagaaaat
     3361 gctaagcaag ctattctaag ggaactcaaa gaagaaactg gtatcgttcc tcaatgtcta
     3421 acctttctta atagagagtg gttctctgag cgttcttact ttgtagacca ttttatcgca
     3481 aaatacaatg gtgcaaaaga tataattaca ttgcaagaag gagagaccgt tgactatatc
     3541 tggttaaaac cagaatatat tgatttattt ttatctaaaa acaaattaat accctctcaa
     3601 ataaaactct taaaatcctt aatctaaaaa ctaggcttaa taaaggccta gttttcattt
     3661 gcatcaataa tatcaaatac cttaagcaaa tttttttcat taatctgata cgcagcttct
     3721 tctctgacaa ttggttttgc acaaaaagca attcctaccc cagcggattt gatcattggt
     3781 aaatcattgg caccatctcc cattgcgatg gtttgtgaaa ggtctaagtg attttcagaa
     3841 gcccactctt ttaactttat ttttttcact tctttagtga caatctctcc ctcaacttcg
     3901 ccagtgagaa aaccaccttt gacaccaaga cgattcgctt tgacataatc aacttgtaat
     3961 tgcttagcta agatatcaat cgtctcgtga aaacctcctg atacaagacc aactttcatc
     4021 tgacgcttgt gacactcttt aatcaattca taagcccctg gagtaaaatg tattgaggat
     4081 aatatatctg tcaaaatagt aacaggcaaa cctttaagta gcgctactct tttcttcaaa
     4141 gcttcttcaa aatctaactc gccacgcatt gcagcgtcag tcagtgcagc aacctgtttc
     4201 cccacccctg cttcaatagc taataaatca atagcttctt ccattattaa ggttgaatca
     4261 acgtccatga ctaacaaacc tttaatcatt taaaacctcc aaaacttttt tactattgta
     4321 ccttaaaatt agcacctttc atagatagca attaaaaaaa gaagagtact aactcttctt
     4381 ttttagaaac gaatacgttc ttgtgccttt tcataagacg tcacaaaacg gtcagttaca
     4441 cctggttcca cagtctcaag tgcgtatgaa atttcatcga aagatgcttt atagttatgt
     4501 tcaacttcaa ataagtacaa agcttgttca aagctctttt gcacattttg ttcaaaacta
     4561 cgataacggt ttgaatactg tagtaactgc tctgtgagag tcgcatcttg aacaactaga
     4621 tatgtagctt gctctaaatt agcgatagca tttgtagtca catcatttaa acgtgaaaca
     4681 gcctcaatat caatacgacc gcgactgagt tcattaataa gtgcctcaat ttgagaactt
     4741 gttgtaaaga aagtgcttaa gaagtcttgc gggattcctg gaagattgcg tttttccatg
     4801 aaacgtttaa tcatatggag cttgttaaca tacttatcca tattttggcg tgctcgtgtc
     4861 tcaacgcttt caatagtttt aacggcttca aaaacttcca tttgattttc ttcaacagta
     4921 gtcaatgttt tcaacgtacg ctcaaataaa atttgtagat gagtataagg tgaagcttct
     4981 tgaccaaaat tttctacaga aggtaaaacc tttgtttcaa tttcttccaa atcatttgtg
     5041 aaggaacgta aacttaagct ttcattttca tttaagatat atttagcaga taatcgtttg
     5101 atttcatgcc ccagttgttc attgttagat tttgcatgag caaggaattg aggaataatt
     5161 ttactatcct tatgagctac tttataagca gcaatctcac gttcaaaaat atcataaagt
     5221 ttatcaattt tttcttgaat atgttcattt tcatcgcgag cacgatctag atccaaacta
     5281 actaaaccat ctgaattcga acgaatagcc tcacgaactt cttgaaaacg ttgttcaata
     5341 tccttttcag ggaaatggta gttttcctct aaaagacgac gatatcctgt ctccaaatca
     5401 tctaactgat ctggaaaatc gtcttctaat ttagcaacaa tagctggaat ttgttctgta
     5461 atttgcccta aagcaatcgt atgctcctct gctttattca aaacttcaga agcttcaatc
     5521 gggtcaccag ttgagtttaa ggtcacaaaa tgtgaaaatt cagcctcgat atttttcaac
     5581 tgcttttcaa tttctggcat ggttgtcccg taattatctt ctttttcaga aattgatttt
     5641 tgaagtgttt cgtataggtc caaagcatgt gttacacggg cactattttt ctcttcttgc
     5701 tcttttaaaa cttctaaagc ttcacgaatc gaaacaatat cttcttctat aatagttagc
     5761 tgactatcaa cattatcaat ctcgtgctta gcacgcacaa aattaaatga atcattaaga
     5821 ttttcagctt caactaaatg aaaatcaata tctttaaatg aattagttga taaatcagtc
     5881 catttttggt tccattctct aaaagttgat tgactttggc caatcaaatg aagcaatttg
     5941 acttgctcaa tctcctcttg cacaggaagg tcaactaact cttgttttct agtttctaag
     6001 tttgcaatta atgtatcatt acgttttctt attacgacgc cgactacgta ggcaataatc
     6061 acaagcagga ctatagccac aagcagcaat atgattccgc tcgacattca actctcctta
     6121 taatggtcta attttctaaa aatctactat aacgttaaga ttatagcata cttgaactgt
     6181 ttttcaaagc tatctttgga ctagatatct aagttactat aaacagcatt agcttcaatg
     6241 aattcacgtc gtggttctac tcgatctccc attagcatat caaaaatctt atctgcttct
     6301 gctgcatcat caactgagac acgtgccatc aagcggtttt caggatccat tgttgtttcc
     6361 caaagttgat ggtcatccat ctcacctaga cctttgtaac gttgtacagt aggttttgaa
     6421 cgtcctgaac tataggtatc tagagcttga cgtaattcct cttcttgatt gacccctggc
     6481 tgaatataag ctttaatctc acttcctact ttgacaccat agattggtgg ttgagcgata
     6541 taaacatatc ctgcttccaa aacaggtcgc ataaaacgat aaatcagagt taaaagtaaa
     6601 gttctgatgt gagctccatc aacatcggca tcagtcataa tgactaattt ttgatatctg
     6661 acttttgata catcaaaatc tgcaccaaat ccagttccca tagctgtgaa gagacttcgg
     6721 atttcttcgt ttgctaaaat tttatccatt gtagcttttt caacgtttag aatcttaccc
     6781 ctaatcggta aaattgcttg aaactcacga ttacgtcctg attttgcaga ccctcccgca
     6841 gaatctccct ccactataaa aagttcattc atctcagcat tattagatga gcaatcagcc
     6901 agtttcccag gtaagtttga aatctctaag cctgactttt tacgtgttac ttcacgcgcc
     6961 cgttttgctg caatacgggc tttagatgcc aaaattcctt tttcaacaat cttcttagct
     7021 acttgtggat tttctaatag gaatcgattg aaagcttcgc taaagagacg atttgtaatt
     7081 ttaacaactt cagaatttcc aagttttgtt ttagtttgtc cttcaaattg tggatttgga
     7141 tgtttaacag atataactgc tgttaaacct tcacgaacat cttctccagt taaattatct
     7201 tcattttctt tgagaatctt attcttttta gcgtaatcat taatcacacg tgtcaaggca
     7261 gtacgaaatc cttgttcgtg cgttcctcct tcatgagtgt ggatgttatt tgcaaaactc
     7321 ataacagtct cttgatatcc agtagtatac tgcattgcta cttcaactga aataccatct
     7381 aattcaccgt cagtataaat aggattttca aaaatcactt ctttgttctc attgataaac
     7441 tcaacgtatg aacctatacc accttcatag tgatattctt tttcaacttc ttgaccttct
     7501 cgcttgtctg aaatggagat tcttagtcca cgatttaaaa aagctaattc ctgaatacgt
     7561 ttggctaatt tatcaaaatc aaatacagtg gtttcagtga aaatttctgg gtcaggcgta
     7621 aagtgaactg ttgttcctga taagtcagta tcaccgataa tttcaagatc attaacaaca
     7681 acaccgcgtt ggtattcttg ataatgaacc ttgccatttt tataaacctt gacatctaat
     7741 tgtgttgaca atgcattaac aacagatgac cctacaccat gtaagccacc agaaaccttg
     7801 tatccgccac caccaaattt accaccggca tggagaactg taaaaactgt ctctacagct
     7861 ggtcttccag ttttttcttg gatatctacc ggaataccgc gaccatcatc aacaactgta
     7921 atagagttat ctggttcaat gtaaacttta atatgtcctg caaatccagc taaggcttca
     7981 tcaattgagt tatcaacaat ttcccataca aggtgatgaa gcccttcttt cgaagtcgaa
     8041 ccgatataca tgcctggacg catgcgaaca gcttctaaac cttctaatac ttgaatctga
     8101 cttgcgtcat attcttgagc acgttgttcc atattctttg tttcttctgt catttactct
     8161 attatctcca tcaatgattt tgacccatcg actaataagg tatcaaaacc agccgattct
     8221 cctgcttgct tatctatatc acgatcgcct attactaaac aattatcaat ttggtatttt
     8281 tctttcaaat aaagcatact ctcgggacta ggtttccttg aaaagccgtt atcagcagtc
     8341 actacttctg taaaatagtc aataatttct gttttttcca gaataactat aacttgatta
     8401 tcacgatgag acaccaaaaa attacgacta ccttgtcttt gaattttcgc taaaagttct
     8461 tttgcccctt ggaatagaat tggtgtttcc aaaaccttag cttcattagc cttatactct
     8521 tttaaaaaat cagcttcttc agggataaaa tatgcaattg catctgctgt tgactctttt
     8581 aatttttgat acacactatc gtggtctgct tgatatccaa attctttcaa agtctctaca
     8641 aaagcacgtg tggaactttc gtaattatca agtagtgtac cacccaaatc ccaaatataa
     8701 tcatgataat tcataccctt tattatatca tattattacg attttttata gctaccacct
     8761 tacttaatta actcactcct ctaaaatatt tcgattatta aaaggctgga ttatctttcc
     8821 aacctttttt tgttatatac tacgatactt caaatgtgat ccattagcct tggcctgacg
     8881 aactgtattt tgataagaca tggataagac caatccaatt ccaataagat tacttagtaa
     8941 tgaacttcca ccttgcgaaa taaacggaag tgggattcct gttagaggta atataccaac
     9001 tgctgcacca atattttcaa aaacatggaa aacaatcatc atgataaaac cagttgatat
     9061 aaaagtataa aattgattat tagattcaat ggtgatcctt aacatacggt aaatgataat
     9121 gagatataac cctaaaacaa tcgccgaacc aatgaagcca aaattctcag caatgacagt
     9181 aaaaatcatg tcactttcac gaacaggaac cgagagctct aaaatattaa aacctttacc
     9241 tgttaccccg ccacttccaa ttgataccat accctgtgtt tgttgataag caattgagtc
     9301 tgcaaaacta aatggatcaa tccaagcaga taaacggttg atttgatatg tatccatccc
     9361 caaattataa aaccattcct tgccgtttgg tgagataaaa atcatcaaaa agctagctat
     9421 aaaaagaacg atagtagata aaattggcaa gattatccac caagatatgc ccgataaaag
     9481 gattaaacct gacaaaatgg caaggaaaac taatgctgtt cctaaatctt tttgtaacat
     9541 tagaagaatc atcactggca atgtaaccaa accaaacaat cctagcaact tccaatcatc
     9601 ttgaaaagtt ttgcgatttt tctgatgaaa actaacagtt attcgagata acattaaaat
     9661 ataggaaatc ttcataaact ctgacggttg aaacaaagtt accgagccaa tggtcaccca
     9721 atttttagca cctgttgccg caaaaagctg tggactataa aaaattaagg gaagaaccat
     9781 tagggttagt cctaaagcat acagataggg cgtggctttc cataaaaatt cagtactgaa
     9841 aagcataacg acaaatgcaa taatacaccc cataataatc caagacacct gctgaaacat
     9901 cgcgatatag atattagaag gataatcatt catcgttgca acataaattg aggctaaacc
     9961 aatcattaat aaaaaaaata ctggtatcac aacagcataa tcaatttgcc ctttaaaata
    10021 gtttgatttt tgtgacatag g
//
LOCUS       DATROY010000070         9994 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.071, whole genome shotgun sequence.
ACCESSION   DATROY010000070 DATROY010000000
VERSION     DATROY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(3..2072)
                     /locus_tag="VBD11_001319"
     CDS             complement(3..2072)
                     /locus_tag="VBD11_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO6954269.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(2074..2310)
                     /locus_tag="VBD11_001320"
     CDS             complement(2074..2310)
                     /locus_tag="VBD11_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954270.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            2477..3430
                     /locus_tag="VBD11_001321"
     CDS             2477..3430
                     /locus_tag="VBD11_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6954271.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            complement(3480..5375)
                     /locus_tag="VBD11_001322"
     CDS             complement(3480..5375)
                     /locus_tag="VBD11_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6954272.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            5523..6506
                     /locus_tag="VBD11_001323"
     CDS             5523..6506
                     /locus_tag="VBD11_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6954273.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            complement(6552..7715)
                     /locus_tag="VBD11_001324"
     CDS             complement(6552..7715)
                     /locus_tag="VBD11_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954274.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRFEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            7827..8471
                     /locus_tag="VBD11_001325"
     CDS             7827..8471
                     /locus_tag="VBD11_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6954275.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            8537..9151
                     /locus_tag="VBD11_001326"
     CDS             8537..9151
                     /locus_tag="VBD11_001326"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6954276.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(9223..>9994)
                     /locus_tag="VBD11_001327"
     CDS             complement(9223..>9994)
                     /locus_tag="VBD11_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6954277.1"
                     /translation="SNQIVKGIEHITKQKGYQIIVGNSNYDAKSEEDYIENMLNLGVD
                     GFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWVKANNYDAVYDMTQECLNRG
                     YKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLVIEDDDHSKSDIEDFLKAVV
                     PDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFDNIEWTDFSSPKVSTIVQPA
                     YEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
BASE COUNT         3352 a         1959 c         1590 g         3093 t
ORIGIN      
        1 aattattttt ttaaatccaa taattgataa ggtatagaag attgactatc tcttaatgaa
       61 tagctttcta attcattgac attatccctt aaaatttgag actctttaga actaattaaa
      121 ccgtcccttt caaattcaga gattgttctg cgttccaagt aatatcctct tagggtttca
      181 tttaaaatat ctggcgttat gcgtgcaatg acacgttcta caaataaacc tgatttaatt
      241 aattctgcgt ctcgaatgcg ttcatttttt aagaaatcta tcaaagttgc attataataa
      301 ccttccatat cttctagcgt ttctaaaatg aggtcattat ttgataagta caaatctgtt
      361 aagtgctgac gattgacttc tgctaacaac cgtttcctgt ttttgaaatg ttttttccga
      421 tttcgtattc cttgtccaaa tgttaaaaac tcatgaacaa taccacgaat aacacgcata
      481 agaatcgtta aactataagt gatatttgac acaaagctac gattaacttg acgttctaag
      541 tattttaaat aacgctggta aatacgatat tctttgatag aaatattgcc ataacgataa
      601 gcagcttcaa gcccgtcgct ttctatacta agcatcatca gttgcaattc agctaagtcc
      661 ttcttaatgt cactagattc ttgttctaaa atgagatgac gaatacggct attataatta
      721 tctataactg cataaaatga agcttgattt tcagaactct taccgtcttc ttctaagaca
      781 gataatactt ttgttaaaat cgatagacgt attaaataat catctttcac ctgtaaggga
      841 ggcgctaact ttggtaatac taaaagtccc gttaaaaaac tcattaacgt tactgcacca
      901 acagtgaaaa gaatcaaact ataccccaac tctcctatga ctgagtgctt gggtaataag
      961 agtattgttg ctatggaaac acttccttta actcctgaaa aagtcaacag ttgaatctct
     1021 ctccaatact ttttaaatga tttatggtat ttgaaagatc tccaagcata aaagcgagaa
     1081 actgctagaa agcgaactag aaaaagtgtg caagtaacta aaacaacaat actaataaga
     1141 tcaaaattac tataagcgtt acttgttaaa agaggaccaa tgatacgtat cagttctctt
     1201 ccaaatatta agaaaactaa accatttaaa ataaaattta aggtttccca aataacagag
     1261 gtaacgcgat ctacttgagc atcaaagaga gtcacttttt tcaaacgatt tgcttgcatt
     1321 actcctgcca ctaccaccgc aataatagct gaaaatccca acaaatcagc aacgaaataa
     1381 gcaacaaaag gtaaggttaa ttccaaaagt agagcccctg taacatcagc agcatcaaat
     1441 ttctctagga cagtgagaca taacctcatt agaaaagcaa aaagcaagcc aattaaaaaa
     1501 ccacccatta tggccaatgc taacttgaga ctagcttttg ctaaggagaa ataacctgtt
     1561 acaagggctg ttaaagcaaa ctggaaggaa actagaccac tagcgtcatt taaaagacct
     1621 tccaatttga ggatattctc tgctcgttta ggaaattgaa acctcttagc aatcgatata
     1681 aaggcaacgg catctgttgg gcctaaggct gctccaagcg caaagcatgc cgcaagtggt
     1741 aaactaactg gcaaaaggtg ccctgctaca taaccaacga caatagctgt taaaaatacc
     1801 gttggtaaga ttaagtaaag aataatagtc cgatacttta caaaactacc aatatcactt
     1861 tcttgacctt ccctaaagtt caaaggagca ataacaaagg caagaaataa ctcaggatcg
     1921 atatggactt gtgacttatg aaaaactaag ccagagagaa tcccaaagac caactggata
     1981 aatggtaatg gtaatttagg aaatatccta ttgataacat tagataaaat caaaaacagg
     2041 agaaagctga ctattagaat agtttcttcc atattattcc tccttcataa catctatcaa
     2101 ttctttcttc aatttctcaa ttttatcatc gattttttta gtatcttctc tctccaatcg
     2161 acagcgacat ctttcttttt caagtattat taatttcttc tttttcttct ttactttact
     2221 taagtgaaca tttgaaaatt cttgattttt agaagaaagg tatttatctt ttagtcgatt
     2281 caatcgttct tccaaagata attgagacat catgacctcc tatttaataa ccaaattatt
     2341 aataataagt attttttctt tcatcggcta tctctagtat aacacgcatt tcttaaattg
     2401 atagaattta agaatatcgt tgctcattat ctccatatgt ggtagactaa caacaattta
     2461 aataggaggt taaccaatga aaaaattaac aaaaggcagc cacattcgtg ttcttagtcc
     2521 ctcttcttcc attgaacgtc tgggtggttt tgaggctaac ctattagcca aagaagtact
     2581 agaaaagctg ggatttcaag tgtcattctc taagcactat ctagaaaatg atatcctcta
     2641 ttctgcttcg attgctagcc gcgtagaaga tttacatgaa gcatttgccg acccttctgt
     2701 tgatgccata ttagcaacca ttggcggatt caattccaac gagcttttac catatttaga
     2761 ttatgattta atctctaaga atcccaaaat aatttgtggt tactcagatt caaccgcctt
     2821 ccttaatgct atttttgcca aagcaaaaat ccaaacttac atgggccctg cttattcttc
     2881 tttcaaaatg aaggaaggtc aaccttacca aacacaagct tggcttactg ccatgacaga
     2941 aaaccactat gaactttggc ctagtgaaga atggtctagt gatccctggt atgatcccag
     3001 caaaccacgc caatttttcc caacagaatg gaaaatttat aatcacggaa aagcttccgg
     3061 tacgattatc ggaggtaatc tctctacgtt tggcctactt cgaggtaccc cttatgcacc
     3121 gaaaattgaa cgctatgtcc tcttgattga agaggctgag gaaagtaatt tttatgaatt
     3181 tgaccgcaac cttgctgcaa ttttacaagc ttatcctcat ccacaagcta ttcttatggg
     3241 gcgctttcct aaagaatgcg gcatgactcc acaagtcttt gaatatatcc tttcaaaaca
     3301 tgctattttc aaggaaattc ctgttattta tgatatggat tttgcgcaca cccaacctct
     3361 tttgacagtg actattggag ctgagataag cgttgacact actacattat ctttatctat
     3421 aaaagaataa aaaaatactc ctgctactca tctgagtaac gggagtttat catttagagt
     3481 taccaaataa taacacgttt ttcaactgga cgccacatgt tatcaccatc tttaacatca
     3541 aactctttat ggaattcttc aaaattcgtt actgtaatat tagttcgcca ttgtgctggg
     3601 gcgtgaacat ctacactagc taacatttgc atatactcat cacgtgcctt catacgccaa
     3661 attgtcgcaa agttaataaa gaaatccctc gctgaaaaat ctgactcacg ctgcgcagct
     3721 tctaaagcac aagcaactcc acctaaatct gcaacatttt ctgatactgt tagtttacca
     3781 ttgacttttg cgccataaga ttccagtcca tcaaactgct caactacttt atctgtaagt
     3841 tttttgaaag ctttaaaatc ttcatcagtc caccaattat tcaaacttcc atgttcatca
     3901 aatgaagctc cattagtgtc aaaagcgtgt gatatttcat gtgcaatcac tgcaccaatt
     3961 cccccataat ttgctgatga actttgctct aaagcataaa atggtgcttg taaaatggct
     4021 gctgggaaga caatttggtt ttgttgtgga tcataatacg cattgaccat gtgggcaggc
     4081 atatgccatt cacttctatc aactggttta ttccatttac tccaaccata ggctattgaa
     4141 attttgtcta aattagtagc attttcgaca agcgatagtt taggatcaat gattttttta
     4201 gtataagtct ctggtagttt ctcagggtat ccaatgtgtg gcgtgataac attaagtttc
     4261 acaatagctt tttcccgtgt tgactgtgca agccaatccg ccttttctaa gcgtgactta
     4321 tagacatcaa tcatcgttgc aatcttatgc tcaacatcag ctttagcttc tggagagaat
     4381 ttttcccctg cataccacaa acctagagct tgattgtaag gtcctgaagc aaggtagtaa
     4441 gctgcttttt tcttatccat tgcttgtggt gtgcctgata aagctcgact gtaaactccc
     4501 gatttgatac gaatatcatc agttaagtaa gcattatagg cattcgcagc tgataaaata
     4561 agatctgcct ttaataattc ccaattagat tctgagtaat agtcagatgc aaattctgtc
     4621 cagaaacgtt cttcaggaac aataacttta tctggttttt gtcctaatat cttttggaaa
     4681 atagcatcta atggaagttc aggtgctaat tttgtgaaat cttcccagtt ataaggatgg
     4741 taaagtttaa catattccga actctcttcg cgagataaga catattgtgc caattttgca
     4801 tccaaagcta aaaccttatc caagatatct ttaatttcct gttctgaaag gccaaacaaa
     4861 ggcagtaagt cttcttgact tttacgccaa agagcaagga gttcctttcc tttttcatta
     4921 tcttcactat aataagttgt atccggtaaa ataatcccag gcgcttcagc ccaaagtaca
     4981 ttcaattgag cattcataaa gtcaggagcc acaccaaatg gaaattcatt tggtttacct
     5041 tctagttcat attctgcaac ttttgatgca aactcagaga aagattgtag acctttatat
     5101 tcttcaatta atggtaatac tggtgatact cctacctctt cacgtttttg ataatcagct
     5161 gtcattttat gaaaagcaat aaaatgattt aaaatcgtat cacttggttt attttcatca
     5221 gccagccact tatctgtcgt tgacagcatt aatgcttcaa tgtcatcagc aagatcagaa
     5281 aaaccacctg tccttggttt gtcatcagga ataacagctg tcttagccca ttccccatta
     5341 accgcttggt aaaaatcatc ttgatatttt atcattattt tctccttaat atctcatatg
     5401 taatctctta cagtctaaca gaaaattcag agtttgacta ttaaaaatac tctcatattg
     5461 gtgctttctt taaatatcat tttgttattt ttgctatact ataaaccaac aaggaggttg
     5521 ttatgactga aaaatattat aattgggcaa cgcttggaac cggcgttatt gccaacgaat
     5581 tagcccaagc actggaagca cgtggacaaa aattatattc tgtagctaat agaacttacg
     5641 acaaaggact tgaatttgct aacaaatatg gtatccaaaa agtttatgat cacatagatc
     5701 aagtatttga agaccctgaa gtggatatca tttatatctc tactccccac aatactcaca
     5761 tctcattttt acgaaaggct ttagcaaatg gtaagcacgt tctttgcgaa aaatctatta
     5821 ctttaaatag tactgagctt aaagaagcca tagatttagc cgaaactaac catgttgtct
     5881 tagctgaagc catgactatt tttcatatgc caatttaccg ccaattaaaa acattagttg
     5941 atagtggaaa attaggaccg ttaaaaatga ttcaaatgaa tttcggaagt tataaagaat
     6001 atgatatgac taaccgtttt ttcagtcgtg acctagcagg cggtgctttg ctggacattg
     6061 gtgtttatgc actttcttgt attcgctggt ttatgtcaga agcacctcac aacattacct
     6121 ctcaagttac atttgcacca acaggggttg atgaacaagt tggtatccta ctaaccaacc
     6181 cagcaaatga gatggcgact gtcagcctta gtttacatgc aaaacaacct aaacgagcaa
     6241 ctatcgctta cgataaaggc tacattgaac tttttgaata tccgcgagga caaaaggcag
     6301 ttattactta tactgaggat gggcatcaag atattatcga agctggcaaa actgaaaatg
     6361 ctctccaata tgaggtagct gatatggaag aagccatttc aggaaaaact aaccacatgt
     6421 acttaaacta taccaaagat gttatggata tcatgacaca gctacgtcaa gaatggggat
     6481 ttacctaccc agaagaagaa aaatgaagag agcttgacaa tgattgtcaa gcccttttta
     6541 gttaatatac actacatttt cttaaaaaat aggcgtttaa ggaaataaat cataccaact
     6601 gctaaactta aaatagcata aacatagaaa actgcattta tttgtcctag taaatgatca
     6661 aaacaagaca gaagaatagc cgcactagct ccaccaatat tacaaaatac taaaactaat
     6721 gtcgttgcag aaccaaccaa cgaatattca aaacggtctg ctactaggct aaacacagca
     6781 gtgaccatca ggctgtaaag gaaacctgac gccatcgcac taattgttag taccattaga
     6841 ttagaaggca atccaactcc aagcaatgtt aaagaaaata agacgaggca gattggaatt
     6901 aattgtttat gaaacatagc tatcaactga ccaaaactca tacctgctat tattcccata
     6961 agcatcatcg cactaagaac aagactagct tgagctggcg tacccaaacc aaattctact
     7021 actagcagag gtatcctaag attaatccca gtatttatag ttatgacaaa tcctgctaaa
     7081 atagctaagg cgacaatata agtcaaatca gctttaggga ttttttgacc tttagtttta
     7141 atcttgtcat ttgtatcttt caccactttc ttaggcgcaa aaacaagata tagaactaag
     7201 attggtaaag ctaaaaaata aatcataaat gatttagacc aaccaaaagt caacaatagc
     7261 ccaactaagg ctgtcaaacc agcatttcct aaaacttcaa aggaccccct taatcctaag
     7321 agttttcttc tttctttgcc ttgataatat tcactaatca cactaatggc cctagcatta
     7381 ataaaaccga taccactccc caataaaaga cgcgatacaa atactaaaag gtagttctga
     7441 ctgacaaccg gcaatcctcc ccctaatgct gttagcaata gtccaaagat aatcatatgt
     7501 ttctcagaaa gttttttact taaccaaggt gtgataagca agatgaagat aatagccatc
     7561 gaaggaattg aaaataataa ttcaacttgg ggtgatggta agcccttatc tctataataa
     7621 gatatcattt gtggtagggc tggtgatgtc gaaaaagttg atagcaagat aagtgataaa
     7681 gagagtaaac ttagcttctc cataaagatt ttcaagtgac aatacctctt tctgatgtct
     7741 ttttttagat aaataaagta taatcaatat taaactagaa atatatgaca tatgtcctaa
     7801 aactatctca aatagggagt caatacatgg atatagaaaa ctactgccaa gactactgtt
     7861 tggagactat ctttccaaaa cattatttca aggatttaca agtaatcacc ctaactgccg
     7921 gccaatccgt ctgtaaacag ggagaacaat tagaatactt gcactacatc gtcaagggac
     7981 gttttaaaat tgtacgtcga ctctttaacg gcaaagaaca tattttagat attaaaacaa
     8041 aaccaacact gattggtgat attgaacttt tgactaatag acaaattgta tcatctgtta
     8101 ttgccttaga ggatattaca gtcattcaat tatcacttaa aggacggaaa gagaaactgt
     8161 taacagacgc aactttcctc ctaaaactta gtcaagagct agctcaggct tttcatgatc
     8221 aaaacatcaa agcgtctact aatttagggt ataccgtcaa agagttactt gcaagtcata
     8281 ttttagcaat tgaagaacaa ggttgctttc accttgaact aagtagttta gctgattcgt
     8341 ttggcatcag ttatcgtcat ttattaagag ttatccatga tatggtcaaa gaaggcctta
     8401 tccaaaaaga aaaaccaaaa tactttatca aaaatcgttt cgcactagaa tcattaaata
     8461 ttcagccatg aagcgtagct ttatcattta attattttat gctaaactaa atcagagaat
     8521 acagttagga gaaaatatgt ctgctattga taaattagta aaagctagtc atttaattga
     8581 catgaatgac attatccgcg aaggcaatcc aacccttcgc aaagtggctg aagaagtcac
     8641 attcccactt tctgagaaag aagaaattct cggagaaaaa atgatgcaat ttttaaaaca
     8701 ctctcaagat ccaattatgg ctgagaagct aggcttacgt ggtggtgttg gtttagccgc
     8761 accacaatta gacatctcaa aacgcatcat tgccgtcctt gttccaaacg ttgaggatgc
     8821 tcaaggaaat ccacctaaag aagcttatag cctccaagaa gtaatgtaca accctaaagt
     8881 tgtatcgcat tctgttcaag atgctgcttt atctgacggg gagggatgcc tatcagtaga
     8941 tcgtgaagta cctggctatg tcgtccgcca cgcgcgtgta actattgaat atttcgacaa
     9001 aactggtgaa aaacatcgac tcaaattaaa aggttacaac tctattgttg ttcaacatga
     9061 aattgaccac attgatggta ttatgtttta cgaccgtatc aatgagaaaa atcctttcgc
     9121 tgtcaaagaa ggcttactta ttttagaata aggacaaata acctagagat aattttctct
     9181 aggttttatt ttttaatagc aaataaaata agaaatcttt ttttaaaaag tcgactcttt
     9241 ccaaaacatc tggcaatcta caatctgttg gttatcgacg gagtcatctc cttcaatacg
     9301 attaatcaaa atctgtgcta cttgttcacc ttcttcataa gccggctgga caattgttga
     9361 cactttagga gatgaaaagt ctgtccattc aatattgtca aacccaacta aaccaactcg
     9421 tggcatatca aaattgagat ttttcatggc agtaaaaacc attggtaaag cccaacaatt
     9481 tggtgcaaat actaatgtct cctccttatc tggaactacc gcttttagaa agtcttctat
     9541 atcacttttt gaatgatcat catcttcaat caccaaagtg tcataaccga aaccattgtc
     9601 ctttaaagca tccatgaatc ccgaagctct ctcaatacgt gtactaagca agctcgtatc
     9661 agcagtaatc ataataaact ttttataccc tcgatttaga cactcctgtg tcatatcata
     9721 aacagcatca tagttattag ccttaaccca actcgtctta tgttcataaa gttgactatc
     9781 aaaaaatacc attggtttct ttttttcttt taaaatacga gaatactttc gaaaatttga
     9841 tgtaggctga ataatgaaac catcaacacc taaatttaac atattttcaa tataatcttc
     9901 ttcacttttg gcatcataat tactattacc cacaataatt tgatagcctt tttgtttggt
     9961 gatgtgctca attcctttta caatttgatt tgaa
//
LOCUS       DATROY010000071         9652 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.072, whole genome shotgun sequence.
ACCESSION   DATROY010000071 DATROY010000000
VERSION     DATROY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            1340..1609
                     /locus_tag="VBD11_001328"
     CDS             1340..1609
                     /locus_tag="VBD11_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954278.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            1670..2821
                     /locus_tag="VBD11_001329"
     CDS             1670..2821
                     /locus_tag="VBD11_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6954279.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVFFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            3021..4013
                     /locus_tag="VBD11_001330"
                     /pseudo
     CDS             3021..4013
                     /locus_tag="VBD11_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4016..4834
                     /locus_tag="VBD11_001331"
     CDS             4016..4834
                     /locus_tag="VBD11_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6954280.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            4836..5621
                     /locus_tag="VBD11_001332"
     CDS             4836..5621
                     /locus_tag="VBD11_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954281.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            6246..6551
                     /locus_tag="VBD11_001333"
     CDS             6246..6551
                     /locus_tag="VBD11_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6954282.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            6551..7399
                     /locus_tag="VBD11_001334"
     CDS             6551..7399
                     /locus_tag="VBD11_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6954283.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            7396..8118
                     /gene="fabG"
                     /locus_tag="VBD11_001335"
     CDS             7396..8118
                     /gene="fabG"
                     /locus_tag="VBD11_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6954284.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            8111..8416
                     /locus_tag="VBD11_001336"
     CDS             8111..8416
                     /locus_tag="VBD11_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6954285.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            8400..8876
                     /gene="fabZ"
                     /locus_tag="VBD11_001337"
     CDS             8400..8876
                     /gene="fabZ"
                     /locus_tag="VBD11_001337"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6954286.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            8866..>9652
                     /locus_tag="VBD11_001338"
     CDS             8866..>9652
                     /locus_tag="VBD11_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954287.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFK"
BASE COUNT         3213 a         1345 c         1837 g         3257 t
ORIGIN      
        1 aagtcagcta accgatcttt ctattctaaa acttatattg ttgctttaga aattttaaag
       61 agcgtggatg gcttcataat aaacagaaat cttaccaatt tgcgaagcca aaaaatatga
      121 tatttgaaga gtccaaagat ctaatagata gtccagttag agaagcgttg attataagtg
      181 ataaggattt tcaaaaatta aaacaagagc tattatttta accgacttat tttaaagact
      241 tatcatatct aggcttgctt gatgattcgg aaaaatacgg agactatact atttcaagga
      301 aaagatacaa aagtttcgaa tcaagtcttc aactatacat ccttcaaagt catcggctag
      361 agatttggaa ttatgaacca atccctttga ttactagaaa aataaatagc ttggagaggc
      421 taacgtgaca ctggttgatc caatctcgct ttatttaaca ctaagaatga tgaagaccct
      481 cgtattgaag aagaagttga gcagctagaa gataagattt tgagaatata atcgtttttt
      541 cgtgctacac taacttgtca gggttgcaac agacaaaaaa ataatttagg agggattcgt
      601 tatgcgtaca aagtatatag gatatgaaat tgttttaagg ggtggttatt aatatggata
      661 agaaaaaggt tgtttcaact atcgtatcta cagggtttct tatagtttca atatcaaatt
      721 tttttagaga gctatcacct atactttttc tcttgaatat tattggattt ttctgcttcc
      781 tattattatt ttctgccaac ataaataaat tgttcttaaa acgatagccg ttatatagga
      841 tatggtatgt atgcatttgg tgaaattata ggtaaaacga atggaatagt acttactcct
      901 caaagctatc gtgagagata taaaaggata tcgaggagtc actagatagt tggatcacat
      961 tgaaaaaatt tgtttaaagt atctcatata agcataaaac gaggaatcaa tcaaaatttc
     1021 ttgattgatt ccttgttttt tggctctttg tcaactgtag tgggttgatg aaaaattata
     1081 ttctggagaa aaccaaattg gttttctcct ttttgatatt caaagtgata tgaattctct
     1141 ttttaaaata ttcaaagttt ctaaagctaa atgtttgtgc ttgatgattt tgatgagatt
     1201 attggttgct caagtttggt gtttgaataa ggcgattcaa ggacattgat aaccttatct
     1261 ctgtctttaa aaaatgtcct aaaaacagtt taaaagattg gagtgacact agagaaagtg
     1321 ttttaaacag ggtaaagaaa tggtgagctt gcttctcttg aaagtaaagc aggaggagtt
     1381 gatagagttt cataatgttt tttatagaga agcgatatct tcttatcttg aaacagaaaa
     1441 ttttgagaag gctaaagtga ctgaagagaa ttaccaagtt tttttaggaa tccgtactta
     1501 tcagaagagt tataactatc aagatcatag tgagaatcta aaaggaaata ttgaggtagc
     1561 aatgatagga gttgaaaata atattagcga cgaacatatt ttttgctaga atatataaaa
     1621 tgtctttgaa ataatttagc cctgtgttat gatattaagg aggttattta tggtacgtaa
     1681 atttttaaaa tcgctcctat ctctctttct tatagcagta atcgccaccg gtatttcagt
     1741 ggcatgtttc ttttttattc cagaaaataa agggaatatt acaccgattt tattgcatcg
     1801 ctttatgcga aaaaataatg ttaatggaat gatgattgtt agcgataata ctggtaaacc
     1861 gattaccatc tcgcatggga ttaaccgtgg ggaagttgaa acggatattg aaaataataa
     1921 attatttcca atggcttctc tccaaaaact catgacagga attattatcc aaagattaat
     1981 tgatcaagat gtcttgagtg aagacgaccg attgtctcag ttctttcccc aagtaaaagg
     2041 tagtaatagt attaccatcc accaattgtt gacccatacg agtgggttaa gagaaaaagg
     2101 tgttaaggtg tctccttatc taaaaaatga gagagaacaa ttacaatttt gcttaaaaca
     2161 ctataacttt gttaataaga aatcgtggta ttattctaat atcaacttta gtttcctaac
     2221 aggtatagcc actcaagtta cgggaaggac ctacgctgaa cttgttgatg atgtgataaa
     2281 aaatcctttg cgattagatg atacccaaag ttaccaatca gttgttaatc atgacttagt
     2341 tagcccaatg cgaaagaacg gcaagttaaa taaaattaat atctttaatc aagtatccac
     2401 agcatatgga gctggagatt ttttcacaac tccgctgaac ttttgggtgt tgatgcggtc
     2461 tttttctaaa gggtactttt tcccaacgga tgaatatacc aaacatcaaa atgatgcgat
     2521 atcacattat tatggtgggc tttacatgca tggtcgtata gttaattcta atggggcctt
     2581 tttccagtat tcaagttttg gttatgcaga tctaaagacg catgatacaa tggtgttttt
     2641 tatgaataac aaagtctata gtgatgctag tcacgtggca ccaaaagcat ttgaatatta
     2701 tcaaaaattt atgttcctta ataagatttt ccacttagtc ttttactttg tattttcttt
     2761 ctttttaatg cttttaatac gtcattcgtt taggaaaaga aggtataaga aacgtctcta
     2821 aatcacatga ataaaaaaga aggctaagtt ggccttcttt tttattcaat attaagcagt
     2881 aacaccatct aagaaaacgt ttgcattgaa tttttaattg tgctataata aatagctgtt
     2941 aatcattatt atagtttaat aatctaactg tcgagctgat ttaagctata aaaatactgt
     3001 tgagtaaaag aggtattaat atgtcaatga ttgaagtttc tcaccttcag aaaaatttta
     3061 taaaaaccgt taaagcacca gggttaaaag gagcatttca atcatttctt cgtcctgaaa
     3121 aacacacctt tgaagctgtt aaagatttaa catttgatgt gcctaaagga caaatttggg
     3181 gatttattgg agcgaacggt gcaggaaaat ctacaacaat taaaatgtta actgggattt
     3241 taaaaccaac ttcgggcttt tgccgtattg atggcaaatt gccacaagaa aaccgacaaa
     3301 attatgttaa ggatattgga gtcgtttttg ggcaacgtac gtagttgtgg tgggatttag
     3361 ctctacaaga aacctataca gtgttaaaag aaatctatga cgttcctgat aaagaatttc
     3421 gcaaacgcat ggctttttta aatgaggttt tggaactaaa tgactttata aaggatcctg
     3481 ttagaacttt gtctctcgga cagcgtatga gagcggatat cgcagcttct ttattacata
     3541 acccaaaggt tcttttctta gatgaaccca ccattggctt ggacgtttca gtgaaagata
     3601 atatccgacg tgcaatcaca caaattaatc aagaagaaga gacaacgatt ttactaacga
     3661 cacatgacct cagtgatatt gaacaacttt gtcatcgtat ttttatgatt gatagaggac
     3721 aagaaatatt tgatggcaca gtttctcagc taaaagagac atttggaaag atgaaaactc
     3781 tttcttttga tttgcgccca ggacaagagc atatcagctc tagtttaatt ggaaagtcgg
     3841 agattaatat taaacgtaat gacttagttt tagatattca gtatgatagt tctcgctacc
     3901 aaactgctga cataattcag cagacattag cagatttttc tgtacgtgac cttaaaatga
     3961 cagatgcgga tattgaagat atcattcgtc gcttctatag aaatgagcta taaggatgaa
     4021 cagaatttgg agacgttata aaccttttat tagtacagga atccaaggat taatcacata
     4081 tcgtgttgat ttcatccttt ataggatagg tgatgtgata ggagcatttg ttgcatttta
     4141 tctatggaaa gcagttttcg attcctcaag tcaatcctta atccaaggtt ttcagctgtc
     4201 agatatgatt ctctatatta ttatgagttt tgtgactaat ttattaacaa gaactgatag
     4261 ttcatttatg ataggtgacg aagtaaaaga cggttcaatt attatgcgac tcttacgtcc
     4321 agttcacttt gctgcttcct accttttcat ggagattggt tctagatggc tcatcttttt
     4381 aagtataggg gtaccgtttt tgcttgttat tacgggggta cgcctcttct tggggactga
     4441 tttgattcaa gctatagtgt tagtggtatt ttatattatt agcattattt tagctttttt
     4501 gattaacttc ttttttaata tttgttttgg cttctcggca tttgttttta aaaatttatg
     4561 ggggtcaaac ctgttaaaaa attcgttagt cgcatttatg tcggggagct tgattcctct
     4621 tacttttttt ccgaaaatcg ttgctgatat tttaggattt ttaccatttt catctttaat
     4681 ttacacacca gtaatgatta ttattggtaa atatgatggc agtcaaattg tgcaagcctt
     4741 acttttacaa atattttggc taatagtaat ggtagcttta tctcaattaa tatggaaaaa
     4801 agtacagtcg catatcacta tccaaggagg ttgagatgac aaaatatcag cgaatgcact
     4861 ttatttttat taaacaatac atgaagcaaa tcatggagta taaaatagac ttttttgtag
     4921 gcgttttagg agtattttta acacaagggc taaatctttt atttctgaat gttctttttc
     4981 aacatatccc ttctttagaa ggatggactt ttcagcaaat agcttttatt tatggttttt
     5041 ctctcctccc aaaaggaatt gatcaccttt tctttgataa cttgtgggct cttggtcaaa
     5101 ggcttattcg aaaaggagaa tttgataagt atttaaccag gccgatcagt cctttatttc
     5161 atgtcctagt tgaaaccttt caagtagatg cattggggga gttattggtt ggttttattc
     5221 tgttatctac cacagtatct agtataagct ggatcgtgcc aaaagtatta ttatttatat
     5281 ttattatccc ctttgcaact ttgatttata cctccttgaa gattgctacg tcaagtatcg
     5341 ctttttggac caaacaatct ggtgctgtta tctatatttt ttatatgttt aatgactttg
     5401 ctaagtatcc tgtcgcaata tataataatc tattgcgttg gatcattagt tttgtcattc
     5461 catttgcttt tacagcttat tatcccgctg cttacttctt acaagataga aatgtctatt
     5521 ttaatattgg tggggttatc ttgatttcct tgatttcttt tatggtatca ctaatacttt
     5581 ggcataaagg agtagaagta tatgagagtg cgggttcttg attagtaagg caagccatta
     5641 atttttttga gtgtttagaa gttttaggct tactaactta gctttgttag tgatgatata
     5701 ctccccttat agtgtctagt gaatttttgt ttttttactg tccactataa ggggagtata
     5761 tcaggtgaag agggactatt agtgttgaga ataatgtccc tcatagaaca ataattacca
     5821 tatctttacc aaaataaagt taaaaacgct cttagatgtg ctttctaaga gcgttttttg
     5881 gtctataaaa ggtaagcaaa aagcagatgt catttatgaa agtgttgaca aagatgaaat
     5941 actttgttac tattttaaca taataatgat attttaatta gagtgtgaat ttataattaa
     6001 tttttacaat gtttatttta aatttaaact aatgtttata gcagtgtttt actcatgatt
     6061 ttgtgaattc aacacttaag tcatgacctc attttaaaga gtttcagacg acacagaaag
     6121 gaagaaaaca taagaaaggc aatgcacggc ttgtccatag tagtgtttga agtagatgtt
     6181 tagaaatatt ttattttcca acagtactga tatcagagcg aagatgatta gttgaaggtg
     6241 ataatatggg acgttctaat attttgagta ggtgtgaaca atgttctgat aatcaatgtg
     6301 gcttgtataa aacgcggctt aaagacaagt tactattagt agtccaactg gttggaaata
     6361 ttaggtattc tgagtttctt acggaaggtg gtgtatatca taagggagaa accttgttgg
     6421 aagttataac cttaggaatt tcttatgaat ataaaagtta tggggatatc aaagtaatca
     6481 agaagtactg tgctgatggc agtgtcgttg attataacat gccaatacta ttaatggaaa
     6541 ggattgaata atgaaaacag taataatata tggaggacag ggtaatgttc agtcaaaaca
     6601 cctttgtcaa ctagttaaaa ctccaaaagg tgaaattctt ttttcaaagt tacaagcaac
     6661 tgatttagaa tcatatcagt taatgatgga tgtcattaac cacaaggtaa gtttagacaa
     6721 tgttcatgcg tctatgttag caaatttttt atataatgat tggctaacaa acgatgaaaa
     6781 aatagtatct gggataggat tttcagctca tagcgccggt attttcaatg tgttatatgc
     6841 tagtggtagt actagttttg aaaacattat tcactttatt aaacgtcgtg cccaacttat
     6901 agaaaattgt catgtgtctg aagagctttg gcttttgatg gttgaaaaca ttgatatgtt
     6961 tagtaaggat gttttaaaag aacagtctga gaaagtaaaa ttagctatta ttactgatga
     7021 tagagctggt gtaattgcta tggatcaagt caaccgttct ttctttgatg acgtgatgac
     7081 taacaaaggt tatttttata aattaaagca gttaggcgta aaagcaccat atcatactga
     7141 ttttttaaat gaatctaaag agcagtataa acaattgatt gaaagcctag atatttcgca
     7201 aaatgatgac tatgactata tttttcattg tgatcaattg gtagatgaaa ttttatatca
     7261 gtggaccaat ctctttaact ggcgtgcgat taaggaaact atcatagaaa aggatgcgga
     7321 gattattgat atttctccta ataggtttat ttctagtcag ttgcaaaaaa tgaaaaagag
     7381 ggggaggaaa cagttatgaa agtagcaata gttactggtg gtaccagagg aattggaagg
     7441 gctattacaa aagagttata caaagaaggt tacaaagtca ttgctattta taatagcaat
     7501 gatgctaagg caagagcttt acaagaggag ttaccaaaat tagacgttta taaatgcaat
     7561 atttcagatg caaaagcagt acaaaaattg gtgactaaaa tttttagaga atatggcggt
     7621 attgattgtt tagttaataa tgctggtatt gttagagatg gctttttctt gatgatgtct
     7681 aaggaaaaat ggatggatgt cattaacata aatattatgg ggctagttaa catgagtaaa
     7741 gccgtgttaa aaatcatgaa ggctaagcgt atacagggaa aggttatcaa tatttcttca
     7801 actagtggta tagcagggca gatagggcaa gcgaactatt cagcaacgaa aggcgctatc
     7861 atttccatta ctaaaactct agcgaaagaa tttgcatcag atggtattac gattaattgt
     7921 gtaagtccag gattcattga aacagatatg acaaatgaat tgcaaaataa agaagagcta
     7981 aaagagcatc ttattcctct aaaaaggttt ggacaacctg aagaggtggc ttggctcgta
     8041 agctttttag caagtgagaa ggcaaactat attacaggaa aaaatattgt gattgatgga
     8101 ggaatgatta atgattaact gggctgaaga acgtaaaata ataaaaacac gtcaagaagt
     8161 agttgatgaa attaaaggta ttttgattga ctctttaatg ttggatattg ataaagatct
     8221 gattatgaat gatcaaccat tatttggtcg tggattagag ctagactcta ttgatgccct
     8281 agaactgtca atagggattt caacaacgtt tggtgttgaa ctaaatgatg atgatatttc
     8341 aatattatca tcagtaaata gactagcaga ttttgtgatt gaaaatagtg aggatataga
     8401 tgaccaagct aagtagtgaa gaaattataa aaatgttacc tcatcgctat ccgtttctaa
     8461 tggtggatag agtgcagagt tataaaaaag gtatcattga atgtcgtaag aatattacta
     8521 tcaacgaacc ttactttcaa ggacattttc cacaacgtcc tatagttccg ggtgttttga
     8581 tgattgagat ggcagctcaa tctgcagctt tactttatat tttagatgca cttgatggta
     8641 gcctcccctc tcttgatgaa ttaacagcag agaaaatagc agaaaaagta ggttatttag
     8701 ccagtgtgaa gaatttcaaa ttcaagaaaa ttgttactcc aggagaccag ctaaacatta
     8761 catgtaaatc tcaatcaaaa ttggggcatc ttttagaaat tcaagtcgtt attagagatg
     8821 agaataaaaa agaggttgcc tcaggaagga tgttggtatc agaaaatgga aattaaactc
     8881 aaaaatattg gaaaaaaata tggttctttt gaagcattga gagacattaa tctcatcttt
     8941 gaagagggaa aattttatgg ccttcttgga cctaatggag ctggtaaaac aactttattt
     9001 aaccttttaa ttcaaaattt taagcaaacg tcaggcgata ttaagtggga ggtggatgga
     9061 aaacctctat ctattaaaga tttttatcgt cacattggta ttgtcttcca aagtaatcgc
     9121 ttagatgata atttaacggt tgaggaaaat ctaatttcta gaggtgcttt gtacggttta
     9181 tctaaatcac aagtacgtaa tcgccttaaa gatcttcaaa cgtatctaga tatcacagca
     9241 ataaaaaaac aaaaatatgg cagtttatca ggaggtcaaa aacgaaaagt tgatattgca
     9301 cgcgccttgc tgccacaacc ttctctatta ttattagatg aaccaacgac tggtttagac
     9361 cctcaatctc gtcgcgattt atgggatgct atcgcacaac taaatcaaca atcgcagatg
     9421 acagttgtac taattactca ttatcttgaa gaaatgtctg cttgtgatgt tttaaatgtt
     9481 cttattgaag gaaacattta ttattcggga gatattaaaa gctttattga gcaacattcc
     9541 acaacaaact tgaatgtggt attgaaacca gaaaagtcat tagaccaact ttcgatagct
     9601 gattttgtta ataaatgtca agtcctttca gaaagggaga tagtatttaa gg
//
LOCUS       DATROY010000072         9318 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.073, whole genome shotgun sequence.
ACCESSION   DATROY010000072 DATROY010000000
VERSION     DATROY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..335)
                     /locus_tag="VBD11_001339"
     CDS             complement(<1..335)
                     /locus_tag="VBD11_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001933356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6954288.1"
                     /translation="MVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFSDTVIT
                     QVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRHYLM
                     PQLGENNFAT"
     gene            complement(370..1341)
                     /gene="tadA"
                     /locus_tag="VBD11_001340"
     CDS             complement(370..1341)
                     /gene="tadA"
                     /locus_tag="VBD11_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEO6954289.1"
                     /translation="MVQSLAKHVIHQAVEVNAQDIYIIPKGDCYEFYMRIDDERRFID
                     VFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYELSEGRLVSLRLSSVGDYRGQES
                     LVIRTLYSGHQDLKYWFDNIKQMKEVLCARGLYLFSGPVGSGKTTLMYQLASEVFKNK
                     QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAV
                     IRASLTGVMVFSTIHAKSIPGVYDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFE
                     TSNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESSPTF"
     gene            complement(1513..1878)
                     /locus_tag="VBD11_001341"
     CDS             complement(1513..1878)
                     /locus_tag="VBD11_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6954290.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(1992..5642)
                     /gene="rpoC"
                     /locus_tag="VBD11_001342"
     CDS             complement(1992..5642)
                     /gene="rpoC"
                     /locus_tag="VBD11_001342"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6954291.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(5759..>9318)
                     /gene="rpoB"
                     /locus_tag="VBD11_001343"
     CDS             complement(5759..>9318)
                     /gene="rpoB"
                     /locus_tag="VBD11_001343"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6954292.1"
                     /translation="QYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAGLKEVFE
                     DVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKETGEIKT
                     QEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGSTVIPNR
                     GAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVRNTIEKD
                     IHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVGRYKINK
                     KLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKFVYTPND
                     YAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYFLNLAEG
                     IGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQQIINIR
                     PVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVRDVHYTH
                     YGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIVWLTADE
                     EDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVAVATACI
                     PFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKHDGRVIF
                     SDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIADGPSMEN
                     GEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDTKLGPEE
                     ITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERLLHAIFG
                     DKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRKIKVGDK
                     MAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHLGMAARN
                     LGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGVMYMIKL
                     HHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVLQEILTY
                     KSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDNEVELRD
                     LDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
BASE COUNT         2828 a         2039 c         1623 g         2828 t
ORIGIN      
        1 gttgcaaaat tattttctcc taattggggc attaaataat gcctaaggcc aatcataatt
       61 agcaccagaa aagacaataa tatcaatggg taagtagcta cttctattac tttttttcta
      121 actaacaaaa gattagctaa ataagactca atctttagta ggctccttga aatgttacca
      181 tgcaaatcag ctaatgcaac ctgtgtgata acagtgtcag aaaaacctaa ctctcctaac
      241 atttttgata aatcttttcc ctctaataat gccttattca tcctatctgt ataacaatca
      301 gacaataact tactcctctt taagaaggtg accatatcag ttaaagaaaa accactagca
      361 aaaagattat taaaaagttg gactactttc cgttgtttct tgagggataa ttttttcgac
      421 ttgtgcctgt ttcttactga tatgtccttc ttcagccaag atatccactt gtctattcca
      481 cttgtctgat gagtgttttt taaagttact tgtctcaaag tcaattaggc ttcctcctcc
      541 aattaaacgt tgatatgcta ttaattttag actattttct aactcttgat agttaacccc
      601 taattctata agcctatcat agactccggg aatactttta gcatgaatag tagaaaaaac
      661 cattactccc gttaaacttg cacgaataac agcacgggcc gtcgcttgat ctctaatctc
      721 tccgataatt aaaatatctg gacgatgccg taaagacagt ttgattaaag catcataagt
      781 cattccaata tcctcattca attggagttg taacatcttg tcattcttga tttctaccgg
      841 atcttcaatc gtgataattt gcttattttt aaatacttct gaagctaatt gatacatgag
      901 agttgtttta ccactcccca cagggccgga aaaaagatat agccctcttg cacacagtac
      961 ttccttcatt tgctttatat tatcaaacca atattttaag tcctgatgac ctgaatacaa
     1021 agtacgaata actaaagatt cttgaccacg ataatctccc acacttgata gtcgtaatga
     1081 aaccagtctt ccctctgaca gttcatagtc acaagaacct aattgacttc gtcttttttc
     1141 tccaacgttc atgcctgcca caaatttaaa gtgactaata agactagcca tcctattaaa
     1201 ctcaaaaaca tcaataaacc gcctttcatc atcaatacgc atatagaatt cataacaatc
     1261 acctttggga atgatataaa tatcttgagc atttacttct actgcctgat gaatgacgtg
     1321 ctttgctaat gattgaacca tcccgtacct ccttaaccat atattcgaaa ataaaataaa
     1381 aaaatctcac caattggtga gattttttca atgatttctt gaggggtagt aacataaata
     1441 ttatgaagca gaggctgaac aaacctatta tatccccata aaaactatct aactttttta
     1501 aactactttt aattacaaat ttaaagaaca tgccgatggc aactcagcta tcttattaaa
     1561 tacttcaaat cgctcaatag attcttcctt aggaatttct tgccattctt tcaataacat
     1621 taaggaatga tactgttgga ggtactcatc acattcttca caccaacgtt tttcttttat
     1681 actccaaaac gttgccaaca aactagactt actttgaaaa taaggccact tttcctgtcc
     1741 cctatcccac tcctcttgaa aatagagcaa agcttctgat aaggtatcat actcagcaat
     1801 ttcagtaata tcttcctccc aaccttcaat aaaccaccat ggttcccaat caccaaacat
     1861 ttttacaact tgatacataa tacacttcct ttagagagta ttatataaaa aataagaata
     1921 aaataaaaaa tatctgctca ttccaaattc ttgtaaaaaa agaacctgag ttaaactcaa
     1981 gttctttttg attaatcttc tgttgtaggt gttgatactt ccgtaacttc tgcatcaaca
     2041 ggagttcctt caataatctc aacttcatta acagcaagtg gttcaatatt acggtaacga
     2101 gccataccag tacccgctgg aatgatttta ccgataataa cattttcttt aagaccaagt
     2161 aagtgatctt tcttaccacg aatagcagca tctgtaagaa cacgtgttgt ttcttggaag
     2221 gatgcagctg ataagaatga attagtttca agggaagcct tagtaatacc cataaggaca
     2281 ggacggcttg tcgcagggat tccaccagaa ataacaatat ctttgttagc atctgtaaag
     2341 tctgaaatat ccataagtgt acctggaaga agatctgtat ctcctggatc catgacacga
     2401 actttacgga gcatttgacg aaccattacc tcaacgtgtt tatctccgat ttctacccct
     2461 tggctacggt aaactttttg tacttcagca agtaggtacg tttcaacaga taaagtatca
     2521 cgaacttcaa gaagacgttt tggttggata gatccttcag ttaaggccgc accacgagct
     2581 acttcatcac caacttcaac tttcatacgt gctgtaaatg gaacaacgta ttctccctca
     2641 cctgtctgac ctttaacgaa tactttctta gtacgtgttg aagagtcttc ttcaattgca
     2701 acgacttcac ccttaacttc agtaatgact gcctcacctt tagggttacg tgcttcaaag
     2761 atttcttgga tacgtggaag accttgtgta atatcagtat ttgaagcaac accaccggta
     2821 tggaaggtac gcattgtaag ctgtgtccct ggttcaccga tagattgagc tgcaatagta
     2881 ccaactgctt caccaacctc aacagcatca cctgttgcca agttaatacc gtaacagtga
     2941 cgacaaacac catggcgagt gttacatgta aatactgatc ggatagttac ttcctcaacg
     3001 cccgctttaa caactttagc agccatatct tcagtaatta aggtatccgc accaacgaga
     3061 atttcacctg tctcaggatg tttgattgac ttcttagtat aacgtcctat caaacgctct
     3121 tcaagtgtct cagtaacctc tttaccatct gtaattgctg tgattgtcaa accgcggtct
     3181 gttccacagt catcttcacg aataataaca tcttgggcaa cgtcaaccaa acgacgagta
     3241 aggtaacctg agtcggctgt cttaagggcc gtatcagtca tacctttacg cgcaccgtga
     3301 gttgagaaga acatttccaa aacactaaga ccttcacgga agtttgataa gattggcaat
     3361 tccatgatac gtccgttagg agcagccatc aaaccacgca taccggcaag ttgtgagaag
     3421 tttgagatgt taccacgagc tcctgagtcc atcatcataa cgatagggtt ctttggatct
     3481 tgtgtctcaa taagacgttt ttcaagtgct tctttagctt cacgccatgt tgttgtaaca
     3541 gcaacataac gatcttcttc agtcatcaaa cctcgacgga aggctttatt gatatcttca
     3601 actctgtggt gagctgcatc aataatctca gctttattgt caataactgg gatatcagca
     3661 atacccacag ttaaaccagc aagtgttgaa tgataatatc caaggtcctt caaacggtca
     3721 aggaaagcag aagtttctgt tgtacggaaa cgtttgaaag tttctgcgat aatattacca
     3781 agatttttct tcttaaaggg aatattaatc tctaagttat caataactgc ttgaatatcc
     3841 tgacctggct ctaagaagta tttatctgga gttttttctg tcaagtttgc attattaggt
     3901 tcaattaagt acggtaaatc ttcaggcatg atatcgttga agagaatctt accaactgtt
     3961 gtaaccatga ttttatgttt ttgctcttct gtccaaggct tattcggcat tgaatcaaca
     4021 gcgataccaa cacgtgtatg taaatgaaca taaccatttt gatatgccat aactgcttcg
     4081 tcatgatctt tgaaaatcat gccttcacct tcacgaccag catcttccat agtcaggtag
     4141 taatttccta aaaccatgtc ctgagatggt gttacgactg gcttaccatc tttagggtta
     4201 aggatgtgct ctgcagcaag catcaaaaga cgagcttctg cttgggcttc ttcagacaat
     4261 ggaacgtgga tggccatttg gtctccatca aagtcagcat tgtaggcctc acatacaagt
     4321 ggatgaagac gaagcgcctt accatcaata aggacaggtt caaacgcttg gatacctaat
     4381 ctgtgaagag taggtgcacg gtttagaagt actgggtgct ctttaataac ttcttctaag
     4441 atgtcccaaa tacgctcgtc cccacgttca accatacgtt tagctgcttt aacgttccct
     4501 gcgaggtcac gagcaacgat ttcacgcatt acaaaaggtt tgaagagctc aatagccatt
     4561 tcacgtggta caccacattg gtacatttta agtgttggac caacagcaat aacggaacgt
     4621 ccagagaagt caacacgttt accaagcaag ttttggcgga aacgaccttg tttaccttta
     4681 agcatatggc ttaatgattt caaaggacga ctacctggtc cggtaattgg acgaccacga
     4741 cgaccattgt cgataagcgc atcaacagct tcttgtaaca tacgtttttc attttgaacg
     4801 atgatgccag gagcatttaa ttccaacaaa cgtgccaaac gattgttacg gttaataaca
     4861 cggcggtaaa ggtcattcaa gtctgatgcc gcaaaacgac caccatctaa ttgaaccatc
     4921 ggacgaagat ccggtgggat aaccggtaaa atgttaagaa ccatccattc tggtttattt
     4981 ccagattttt taaaggcatc taacacatcc agacgacgaa ctgctttcac acgtttttga
     5041 cctgtcgctg atttcaactc ctctttaaga actgcgattt ctgcatctaa atcaacgcgt
     5101 ttaagaagat cttgaatggc ttcagcaccc attttagcaa caaacgaacc gtaaccgtac
     5161 tcttgaagtt tttcacggta ttcacgctca gtaaggagtg acttcggttc taatggtgtg
     5221 tccattggat cgataacaac ataagccgca aaataaataa cttcttcaag tgcacgagga
     5281 ctcatatcca atgtgagtcc catacgcgat gggatacctt tgaaatacca gatatgagat
     5341 actggtgctt ttaactcgat atgtcccata cgttcgcgac gaacttttgc acgagttact
     5401 tcaacgccac aacggtcaca aatgattcct ttgtaacgaa tacgtttgta ttttccacac
     5461 gcacattccc aatcttttgt tggaccaaaa ataacttcat caaaaagtcc ttcacgttct
     5521 ggtttcaatg tgcggtagtt gattgtttca ggttttttaa cttcaccata agaccatgaa
     5581 cggaccttac ttggtgatgc taatgtgatt tgcatacttt taaaacgatt tacgtcaacc
     5641 actagtttta cctttcttcg atttatcatc atactagccc agctaggatt tcaataaatc
     5701 ctaggactag cattgcatat caattctata ttcgcttcta gttataaact agataaattt
     5761 aatcttcttg aacgacttca gatacttgtt cagcttgttt ttcttcagct tcttgtttca
     5821 cacgcgcttt ttcaaggtca tcaacgtgca tcacatcatc atcttcacct tcatcaagat
     5881 cacggagttc aacttcgttg tcatcttcat caaggacacg catatcaaga ccaagtgatt
     5941 gcaattcttt tacaagaaca cggaatgatt ctggaacacc tggttttgga attggcttac
     6001 cttttgtgat agcttcataa gctttaagac gtcctgtaac atcatcagat ttgtatgtta
     6061 aaatttcttg aagaacattt gaagctccat aagcttcaag cgcccaaacc tccatctcac
     6121 caaaacgttg tccaccaaat tgagctttac ctccaagtgg ttgttgagta acaagtgagt
     6181 atggtcctac tgaacgggca tgaagtttat catcaaccat gtggtgaagt ttgatcatgt
     6241 acatgacacc aaccgacaca cggttatcga atggttcacc agtacgacca tcataaagaa
     6301 cagtcttagc atctgaatcc atacctgctt cttgaacagt ttcccaaagg tcttctgaag
     6361 aagccccatc aaagactggt gttgcaatat gaatacctaa attacgagca gccataccta
     6421 aatgaagctc cataacttgt ccgatattca tacgtgatgg caccccaagt gggttcaaca
     6481 tgatatcaac tggcgttcca tctggtaaat aaggcatatc ttcaacagga acaatacgtg
     6541 agacgacacc tttgtttccg tgacgaccgg ccatcttatc accgactttg attttacgtt
     6601 tttgagcgat ataaacgcgg acaagcatat taacacctga ttgcaattca tcaccatttg
     6661 cacgagtaaa gattttaacg tcacgaacta ctccatcgcc accgtgaggt acacgtagtg
     6721 aagtatcacg aacttcacgt gatttatcac caaaaatggc atgcaagaga cgttcttcag
     6781 cagataagtc cttttcaccc ttaggcgtta ccttaccaac aagaatgtca ccttctttta
     6841 cctcagcacc aatgcggata attcccattt catcgagatc acgtagtgaa tcttcaccaa
     6901 catttggaat ttcacgagta atttcttcag gcccaagctt tgtatcacgc gtttcagatt
     6961 cgaattcttc caagtgaaca gatgtataga cgtcttcttt cacaaggcgc tcactcatga
     7021 taacggcatc ctcgaagtta taaccttccc aagtcatata agcaacaact gggttttgtc
     7081 caagagccat ttcaccattt tccatagatg gtccgtcggc gataaaatca cctttttcaa
     7141 cgagatcacc aactttaact aaggtacgtt ggttataagc agtacctgag tttgaacggc
     7201 ggaatttttg aacatgataa acatcaagag aaccatcttc gcgacgcact tcaacttttt
     7261 cagcatctga aaaaataaca cgaccgtcat gtttagcaat cacagctgca cctgaatcgt
     7321 gggctgcttg atactccata ccagtaccaa catatggtgc ttttggatca atcaatggca
     7381 cagcctgacg ttgcatgttg gcacccatca aggcacggtt agagtcatca ttttccaaga
     7441 aaggaataca tgctgttgcg acagcaacta cttgcttagg tgacacgtca acaaagtcaa
     7501 caatgcttga aggaaactct tggttattac cttgatgacg acccataacg atttcttctg
     7561 caaatgtacc gtcttcgtta agcttagagt tggcttgtgc aactgtaaat tcatcttctt
     7621 catcagcagt caaccaaaca atttcgttcg ttaccgcacc agtagaacga tctactttac
     7681 gatatggtgt ttgaataaat ccatatttat tgaggtgtcc aaatgaagat aagttattaa
     7741 tcaaaccaat gtttggacct tcaggtgttt caattggaca catgcgccca tagtgggtat
     7801 agtgcacgtc acgaacttca tatccagcac ggtcacgtgt caaaccacca ggtcctaagg
     7861 cagagagacg gcgtttgtgc gacaattctg atagagggtt gtgttggtcc atgaattgtg
     7921 acagctgtga tgaaccaaag aattctttaa cagcggcagt cactggacga atgttaatga
     7981 tttgttgagg tgtcaagact tcattgtctt gtacagacat acgttcgcgg acattgcgtt
     8041 ccatacgagc aagtccgata cggaattggt tcgcaagtaa ttcaccaact gcacgaatac
     8101 gacggttacc taaatggtca atgtcatcta ccttaccgat accctctgca aggtttaaga
     8161 agtatgacat ctctgctaaa atatctgcag gagttaaggc acgaacttta tcttcaggat
     8221 ttgagtttcc aacaatagta acaacacggt ctggatcagt tggagcaaca actttgaatt
     8281 tttgaagaat aactggttca gtaactacag cataatcatt tggtgtgtag acaaacttgt
     8341 ttaagtctcc atcgatatgc tctgcaattg aatcaatgac atctcgtgtc ataacggttc
     8401 cagcttcaac aagaatctct cctgtttcac catcaactaa attttcagcg attgtttggt
     8461 tcaataggcg tgttttaaga ttcagttttt tattaatctt ataacgacca acagccgcta
     8521 agtcataacg acgtggatca aagaaacgtg caactaaaag actacgtgag ctgtctgcag
     8581 tttttggttc acctggacga agacgttcat agatttcttt aagtgcttca tctgtacgag
     8641 aatcacttgg atttttatga atgtcttttt caatagtatt gcgaacaagt tcactgtcac
     8701 caaagatgtc aacaatttca tcgtctccag agaaaccaag tgcacggact agtgttgtaa
     8761 atggaatttt acgtgtgcgg tcgatacgtg tataagcaat atcttttgcg tctgtctcaa
     8821 gttccaacca agctccacgg ttagggataa cagttgaccc ataaccaact tttccatttt
     8881 tatctacctt atcattaaag taaacaccag gagaacggac taattgtgaa acaatgatac
     8941 gttcaccacc attgatgatg aaagtaccca tttcagtcat aattggaaaa tcaccaaaga
     9001 aaacttcttg agttttaatt tcaccagttt ctttattaac taaacggaaa gtaacaaaaa
     9061 taggtgctga gtagctagca tcatggatac gagcttcttc caaagtatat ttaggttctt
     9121 ttaattcata accaacaaat tcaagatcca tagtatctgt aaagtttgaa attggaagta
     9181 catcctcaaa tacttctttc aagccagcat caagaaaatc ttggaatgaa tcagtttgaa
     9241 tttcaattaa gtttggtaaa tcaagaactt ccttaattct tgaaaagcta cgacgtgtac
     9301 ggtgttttcc gtactgaa
//
LOCUS       DATROY010000073         9172 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.074, whole genome shotgun sequence.
ACCESSION   DATROY010000073 DATROY010000000
VERSION     DATROY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9172
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..809)
                     /locus_tag="VBD11_001344"
     CDS             complement(<1..809)
                     /locus_tag="VBD11_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6954293.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
     gene            complement(809..1711)
                     /locus_tag="VBD11_001345"
     CDS             complement(809..1711)
                     /locus_tag="VBD11_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6954294.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(1717..1845)
                     /locus_tag="VBD11_001346"
     CDS             complement(1717..1845)
                     /locus_tag="VBD11_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954295.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1978..3021
                     /locus_tag="VBD11_001347"
     CDS             1978..3021
                     /locus_tag="VBD11_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6954296.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            3125..5287
                     /locus_tag="VBD11_001348"
     CDS             3125..5287
                     /locus_tag="VBD11_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6954297.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            5250..5708
                     /locus_tag="VBD11_001349"
     CDS             5250..5708
                     /locus_tag="VBD11_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6954298.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            5789..6052
                     /locus_tag="VBD11_001350"
     CDS             5789..6052
                     /locus_tag="VBD11_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6954299.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            6280..7215
                     /locus_tag="VBD11_001351"
     CDS             6280..7215
                     /locus_tag="VBD11_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6954300.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            7208..7981
                     /locus_tag="VBD11_001352"
     CDS             7208..7981
                     /locus_tag="VBD11_001352"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6954301.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7996..8394
                     /locus_tag="VBD11_001353"
     CDS             7996..8394
                     /locus_tag="VBD11_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6954302.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            8391..8822
                     /locus_tag="VBD11_001354"
     CDS             8391..8822
                     /locus_tag="VBD11_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6954303.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(8863..9138)
                     /locus_tag="VBD11_001355"
     CDS             complement(8863..9138)
                     /locus_tag="VBD11_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6954304.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
BASE COUNT         3055 a         1426 c         1849 g         2842 t
ORIGIN      
        1 aagacacgat aaacataagg attattaggt ttagaaactt gacgagactc ttcaatgtgt
       61 aagactggta agttttgttg taacaattga ttatactcgg atttaattgt acctctaaca
      121 gttacccaag tattgtccgg ataacttttt tgatttccag ttgttagtag accataaact
      181 cctgaatcag caatacaatg aatgattcca aagcggaaaa taaattgata tccttcgtgt
      241 ttgggttcgt tataaacaaa cccagtatat tgtatttggc gatccatgaa ttgctcggga
      301 tagagataaa ttagttccat aacttccatg tagttttcag ttgtgatggt taatgtacca
      361 gagcctttat attttttaag ctctttttgc atttcttttt cataggctga agaagtaaaa
      421 taagtgctgg tatctggttt caaatattga acgcgtgtcc catcttgact gacagtacca
      481 gtagagccgg ctgctagagg gaaattatat cccttggcag agactgtggt cgagtctaaa
      541 ctaactgtag gtactaacaa acctactaaa acgggaaata ctaaaatcat tgggcttgtt
      601 gatttagcta ttttaccatg taggtgacta tgcattttca tatttttaac ccaaataatt
      661 agttgtacaa ttgctaaaat aaaagatagg accattgaaa tataggccaa ataggtataa
      721 tgagtattaa tatactgatt tagtttacct gataacttta aatacatgct taattcaaaa
      781 taaccagcaa gaataagaaa tcgaatcatt atattacccc cacaaataaa caatagataa
      841 taataataag agaagataca ctaatgaatt ggacaataaa ccttgtttta aaagaattta
      901 ccatcatcat taaattctta atatcaatca tgggtccaat taaaaggaaa gccatcactg
      961 gcgcgattcc aaaggttgat aataaagaag cacctataaa agcgtcagct tcactacaca
     1021 atgacaagat gaaagcaagc aacatcataa ctaagatggc tgtgataggg ctgtgtccga
     1081 tagtagtaag aactcgagtt ggtaaataaa tttgcatagc tgaagcaatt aaagtgccaa
     1141 atactagata gcgtcctgtg tcaaaaaatt catcgatggc atgagctaag gctagaaaga
     1201 ttttttgata ccacttctta tcagaataat catgaaaatg agtaggttta gcgtcttctt
     1261 ttagaatatt atcatctact aaaaaggcaa gcatgactcc taaagcaatg gctacaatgg
     1321 tagcacctac aaagcgtaat atcaggaagc gtatagaatt tccaaatgct gagtaggtcg
     1381 caaataaaac aataggatta ataataggag cagtagcaag aaacggaact gccgtgtatg
     1441 aaggcacttt cttttcaagg aatcgattaa taataggaat gataccacat tcgcaagaag
     1501 gaaagacaaa gccaacgaag gttccaaata agactctgag aaatttattt ttcggtaaga
     1561 acttattgac tatatcaggt gttataaaga cttcaataat tcctgataaa atagttccaa
     1621 gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa aatggcaaac cattgaagga
     1681 cactatccgg taattgatta aagatatcca tagaacttaa tctttctgag aatcatcttt
     1741 ctgttcaggt tttgaatcag aatcaacttt tttaggtttt ggtactttag caaaactagc
     1801 gaacatactt tctaagtcat cttgacgttt gtgcgtaata gccattaatg gcacctcttt
     1861 ctttatataa taacctatta tactatatta gcttattttt aacataaaaa aacaaatcga
     1921 ttggaaatga tactatatag tgataaaatg ttattagtag attgatgagg taagattatg
     1981 attgaattag gtattacaac attcggagag acaacaatcc ttgaagaaac aaaccaaagc
     2041 tattcacatc ctgagaggat tcgccaatta gttgctgaga ttgaactagc tgatcaagtt
     2101 ggtttagatg tatatggtat tggagagcac catcgtgaag attttgcggt ctctgcaccc
     2161 gaaattatcc tagcagcagg agcggttaga actaataata tccgtttatc tagtgcagta
     2221 acgattctct cttccaatga tcctattcgc gtctatcagc aattttcaac gattgacgca
     2281 ctttcaaatg gtagagcaga aattatggca gggcgtggtt cctttattga gtcttttcca
     2341 ttgtttggat acgatttagc ggattatgat gatttattta atgaaaaaat ggatatgttg
     2401 ttagcaatta actcagcgac aaatctcgat tggaaaggtc atttgacaca aacagttaat
     2461 gagcgaccaa tttatccaag agcattacaa agacagttat caatatgggt ggcaacagga
     2521 ggaaatgttg attctacaat tcgtattgca gaacaaggtt tgccaattgt ttatgcaact
     2581 attggtggga atcccaaagc ctttcgtcaa ttggtccata tttataaaga agttggtagt
     2641 cgtaatggac acaaaccaga acaactaaaa gttgctgctc actcttgggg atggattgaa
     2701 gaggataatc aaaccgctat tgaccgttat tttttcccta cgaaacagac cgtcgataat
     2761 attgctaagg gacgccctca ttggtctgaa atgactaaag agcagtattt acgttcagta
     2821 ggtccagaag gtgctatttt tgtaggaagt cctgaagtgg ttgcacataa aattatagga
     2881 cttgttgaag cgcttgagtt agaccgtttt atgttacatc taccagttgg ctctatgcct
     2941 cacaaagacg tcctaaatgc catcaaactt tatggtgagg aagtagcacc aattgttcga
     3001 aaatattttg aaaagaatta aagcagacct ggtctgtttt ttattgttag attataaaaa
     3061 cgctatcttg aaatcgcttt ttaaaactct tcgtaagtca aaaatatgct ataataaaag
     3121 cgttatggaa aatttaaata ttacacaaat tgctattgat ttaggtatta aagcgtcaca
     3181 aatcgaaaag gttttagaat taacagatga aggaaatact attcctttta ttgcccgtta
     3241 tcgtaaagaa atgacaggta atttggatga agttcaaatc aaatcaatta ttgaccttga
     3301 taagagtatg acagctcttt cagatcgtaa aactacggtc ttagccaaaa ttgaagaaca
     3361 aggaaagttg actcaagaac tcaaaaaggc tattgaagaa gcaactaaac tagcagatgt
     3421 tgaggaactc tacctacctt ataaagaaaa acgtcgtacg aaagctacga ttgcacgtga
     3481 agcaggtctt tttcctttag cacgtcttat tttacaaaat aaggataacc ttgaggaaga
     3541 agctcaaaac tatcttacag atggttttga gacaactacg aaggcccttt ctggagcagt
     3601 agatattctt atcgaagctt tttctgagga taataaatta cgttcatgga catataatga
     3661 gatctggaac tacagctcaa tcactgcagt tgttaaagat gagtcacttg atgaaaaaca
     3721 ggtcttcaaa atctactatg acttttctga aaaaataagt aaactccatg gctatcaagt
     3781 tttagcattg aatcgtggtg agaaaatggg tgttcttaag gttaattttg agcataacct
     3841 tgaaaaaatg tttcgctttt ttgctgttcg atttaaagaa actagccaat atatagatga
     3901 ccttattgta cagactgtca agaaaaaaat tgttccagct atggaacgcc gtatccgtac
     3961 agaactaagt gaaggagcag aagatggtgc aatttcactg ttttcagaga atcttcgtaa
     4021 cttattgtta gtctcaccgc ttaaggggaa aatggtgtta ggttttgacc ctgcctttcg
     4081 tacaggagca aaattagcag tagtagacca aactggaaaa ttaatgacaa cacaagtgat
     4141 ttatccagtt cctccagcta atcaagcaaa aatcgaacaa tcaaaaatag agttagcaaa
     4201 acttatcaaa gaattcaata tagaaattat tgctataggg aatggaacag cgagtcgtga
     4261 atcagaagca tttgttgcag aagttttaca agattttcca gacgtatcct atgtgattgt
     4321 taacgaaagt ggtgcctctg tttattcagc ctcagaattg gcacgtcatg aatttccaga
     4381 cttaacagtt gaaaaacgtt cggctatctc aattgcaaga cgtctgcaag acccgttagc
     4441 agaactagtc aaaattgatc caaaatctat tggtgtgggt caatatcaac atgatgttag
     4501 tcaaaaaaaa ttagcagaaa atcttgattt tgttgttgaa actgtagtta accaagtagg
     4561 tgttaatgtt aatacagcaa gtccagcctt gttagctcac gtttcaggtt taaataaaac
     4621 aatatctgaa aatattgtta aatatcgtga agagaatggg caaattaagt cacgtgctga
     4681 aattaagaaa gtacccagat taggagcaaa agcttttgag caagctgctg gattcttacg
     4741 tattcctaat gctaaaaatt tcttggataa tacaggcgtt caccctgagt cttatgaagc
     4801 tgttaaaaag ttgcttgacc aactgactat caaagaatta gatgatcttg caaaagagaa
     4861 gttacaacac cttgacttaa ttgcaacagc agaaagtatt ggggtaggtc aagaaacatt
     4921 aaaagacatt atagaagatc ttttgaaacc aggtcgtgat ttgcgtgatg attttgaagc
     4981 gcctgttttg agacatgatg ttttagatgt cagtgattta aaagttggac aggaattgca
     5041 aggaactgtt cgaaacgtcg ttgattttgg tgcttttgtt gatattggtg ttcatgaaga
     5101 tggtctaatc caccagtctc gccttattaa gcgaaaacgc gataaaaaaa ctagaaaaat
     5161 gcctccttta ccacatccaa gcaaatatct ctctgttggt gatattgtaa ctgtttgggt
     5221 tgtagaggta gatgcggagc gtggcagaat tggacttagt cttatcaagc cagacggact
     5281 tgactaatta tgttaagaag gtgtctatag aagattttgg atgtcagttt ttacatactg
     5341 cacattggaa taatcgctta agaacaaccg gaggacgctt ctttccaaac gatagacacc
     5401 ttgactttaa tcccaaaatt tatcgtgagt ttggtattga tatttttcgt aagattgtaa
     5461 gacatgagct ttgccattat catctctata tacaaggtaa aggatatcga catagggata
     5521 aagcttttaa agagttacta gaaaaagtcg atggcttacg atatacccct agagttacgg
     5581 ctgttaaagt gaattatcat cattactctt gccaatcttg tggtcaagta taccgacgta
     5641 ggagacgtgt taatttacgc aagtttgctt gtggttattg tcatggtcgc ttgatagaga
     5701 gtttttaaaa tcagtcgtaa ggcctattta attttactgt caaagtagtt ataattaaaa
     5761 tatagagtag atagaaagaa ggataggtat gaaatcaagc ttctacaaac aacgaaaagg
     5821 taagttggtc tgcggtgttg tcgccggtct agctgataag tataactggg atttagctct
     5881 ttcacgggtg ttgattgcac tgattttgta ttttaccaaa tttggtcttt tgctatatat
     5941 tttattagct gttttcttac cctataaaga agatattatt gaaacaagaa gacaaggtcc
     6001 cagaagacgt aaagatgctg aacctgttga tgatgacggt tggttctggt aattcatata
     6061 aaggagattt gctagacgaa tctccttttt tcttagcttt atttagacca ttttttcaaa
     6121 atatcttctt tttttcagaa aaacgcgtta gcgcttactt ttagtaaaaa gtatgatata
     6181 atgtgggtgt aaattaatga aagtgtttag tacctttgct caactttaaa ttgtagggta
     6241 tagcaatagg gatattcact tttataagaa ggagtaagta tggcagttac tgttcaaatg
     6301 ttagttgacc gcttaaagtt aaatgtcatc tatggtgatg aacatctatt atctaagcgg
     6361 attacaacag ctgacatttc acgtcctggt ctagaaatga cagggtattt tgattattat
     6421 gcaccagagc gtttgcaact tgttggtatg aaagagtggt catatttaat ggctatgaca
     6481 ggacataatc gttatcaagt gttacgtgag atgttccaaa aagaaacacc agctattgtt
     6541 gtagcacgtg atttagaaat tccagaagag atgtatgagg cggctaaaga taccggtatt
     6601 gctattcttc aaagtaaggc ccctacaagt cgtttatcag gtgaagtttc ttggtattta
     6661 gattcttgct tagctgagag gacaagtgtt catggtgtct tgatggatat ctatggtatg
     6721 ggtgttctga ttcaaggaga ttccggtatt ggtaagagtg agacaggact agagctagtt
     6781 aagcgaggac atcgtttggt agcagatgac cgtgttgatg tttatgctaa agacgaagag
     6841 acgctttggg gagagcctgc tgagatatta cgtcatttac ttgaaattag aggtgtggga
     6901 atcattgata ttatgagcct atatggagct agtgcggtta aagattcatc gcaagtgcaa
     6961 ttagctatat accttgaaaa ctttgaaact ggaaaagttt ttgatagact cggaaatggc
     7021 aatgaagaga tcgaattatc tggagttaag gttcctcgga tacgtattcc tgttaaaaca
     7081 ggccgaaacg tgtcagtagt tattgaagca gcggctatga atcacagagc taaacaaatg
     7141 gggtttgatg ctacccagac ttttgaggat cgtttaacac atttgattag tcagaacgag
     7201 gtcaatgatg attaatccag tagcaataca attaggcccc ttttctattc gctggtatgc
     7261 tatttgcata gtgtctggta tgttgttagc agtttactta gcaatgaaag aggcccctcg
     7321 taagaacatt aagtcggatg atattctaga tttcattctc atggcttttc cactatccat
     7381 tgttggagct cgtatttatt atgttatctt cgaatgggct tattactcta agcacccagt
     7441 agagattatc gccatttgga acggtggtat tgctatttac ggagggttaa ttaccggagc
     7501 cattctcctt gtgatttttt catatcgtcg cttgattaat cccattgatt ttctggatat
     7561 tgctgctcca ggagtgatga ttgcccaagc cataggtaga tgggggaatt ttattaatca
     7621 agaagcatac ggtagggcag taaaaaatct gaattactta ccaaacttta taaaaaatca
     7681 aatgtatatt gatggagctt accgagtacc tacattttta tatgagtctc tttggaactt
     7741 cctaggtttt gtcatcatca tgtctattcg tcatcgaccg cgcactttaa aacaaggtga
     7801 agtggcatgt ttttatttag tttggtatgg atgtggacgg ttcattattg aagggatgag
     7861 gacagatagc ctttatttgg cgggtttgcg cgtgtcacaa tggttatcag ttattttagt
     7921 tattatcgga attgtcatga ttatttatca tcgtcgtgaa caacatattt catattatta
     7981 aacagaggag gcattatgat agaaattgct gtattaatta ttgccattgc gtttgttgtt
     8041 ttagttttag gaatactttt cgttctgaaa aaagtatcag aaacgattga ggaaaccaag
     8101 caaactatta aagtattaac aagcgatgtt aacgtgactc tttaccaaac taatgaaatt
     8161 ttagccaaag ctaatgtttt agttgatgat gttaatggta aagtatctac tattgatcct
     8221 ctttttgtag ctattgctga cttatcagaa tctgtatctg atttaaacct acaggcaaga
     8281 cacattggac aaaaggcaag tagtgcaacc tcaagtgtta ctaaggcagg gtcagcttta
     8341 gctattggaa aagcggcttc aaaaattttt cgtaaaaaag gagacaaaaa atgagtaaat
     8401 tatttaaaac tttagttatc agtgctgcat caggtgcagc agctgcttat tttttaacaa
     8461 caaaaaaagg aaaagagcta aggaaaaatg cagaaaaatt ctatggagaa tataaagaaa
     8521 atccagaaga atatcatcaa atagctaaag ataaagcaag tgaatattca aatttagctg
     8581 ttgatacttt taaagattat aaaggtaaat ttgaatcagg tgaattgaca acagaggata
     8641 tcgtctcagc cgttaaggaa aaaagcggag aagtagttga ctttgctaat gattttgtca
     8701 atcaagctaa atcaaaattc tcagacgagg atactgctaa aaaagaagat aaggctcctg
     8761 aaacaaaagt agaagatatt gtcattgatt ataaagaaaa cacagaagat aaagaaaaat
     8821 aaaaaagaag ctactataag tagcttcttt tttatgagat ttttattgtc tgagtgacca
     8881 aatgaatgaa gagactgcag atgtaactgc tagacttgcc agcatcgcta gtaagaaact
     8941 ccacacaggt gttaaactta gtgctagtaa taagaaagag attagcacag tggttatact
     9001 gaagagagcg atacgagcaa aaaagactag ctcacgcaac ttagcactgt gatcaaattc
     9061 ttgaaatact tgaaattttg gttgagcttg ctctgttttg ctttcgtgag tttgctcttg
     9121 cttaagtggt gatgacatag ctttctcctt tgatactagc atatcatatc ac
//
LOCUS       DATROY010000074         8712 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.075, whole genome shotgun sequence.
ACCESSION   DATROY010000074 DATROY010000000
VERSION     DATROY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            12..1346
                     /locus_tag="VBD11_001356"
     CDS             12..1346
                     /locus_tag="VBD11_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6954305.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(1417..1599)
                     /locus_tag="VBD11_001357"
     CDS             complement(1417..1599)
                     /locus_tag="VBD11_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954306.1"
                     /translation="MLTYKKALVRQVDTKDCGVTALASIAKFYGSKYLLNHLRELAKT
                     NRDGTTASGIIAGTTK"
     gene            1888..2061
                     /locus_tag="VBD11_001358"
     CDS             1888..2061
                     /locus_tag="VBD11_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6954307.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            2130..2978
                     /locus_tag="VBD11_001359"
     CDS             2130..2978
                     /locus_tag="VBD11_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954308.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            2984..4138
                     /locus_tag="VBD11_001360"
     CDS             2984..4138
                     /locus_tag="VBD11_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO6954309.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTS
                     ALLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            4367..5401
                     /locus_tag="VBD11_001361"
     CDS             4367..5401
                     /locus_tag="VBD11_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6954310.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            5648..6073
                     /locus_tag="VBD11_001362"
     CDS             5648..6073
                     /locus_tag="VBD11_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6954311.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            6091..6594
                     /locus_tag="VBD11_001363"
     CDS             6091..6594
                     /locus_tag="VBD11_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6954312.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            6598..7407
                     /locus_tag="VBD11_001364"
     CDS             6598..7407
                     /locus_tag="VBD11_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6954313.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            7404..8231
                     /locus_tag="VBD11_001365"
     CDS             7404..8231
                     /locus_tag="VBD11_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6954314.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            8369..>8712
                     /locus_tag="VBD11_001366"
     CDS             8369..>8712
                     /locus_tag="VBD11_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6954315.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSP"
BASE COUNT         2648 a         1376 c         1645 g         3043 t
ORIGIN      
        1 ggtagaggaa tatgaattct actctatcca tcttagagat agctttaacg ataatattaa
       61 caatatttct cttttctaaa atcagtggac gtcaattgac tttaatagag attggaattt
      121 gtattgctct gagattagga cttgtaattt tatttatagc agctaatact attattaatc
      181 tttcatttct aaagtactgg cttctcccca tctatatgtt aagtctggct ttcttgttgc
      241 taagacctat atctaaaact ttagtagtct tttatggctt gtttccaatt gttctcagaa
      301 atctttgttc acgttcttta gctttttttg tctttccttt gtttaatatt gattttcata
      361 caaccaacca ggggttttgg ggatattttg ttgatgtctt gtctactctt tgtgttctcc
      421 tatttttaag atggatgcaa tacgacttta aaactttgcg aagtagtgaa gtttcgccca
      481 tagataagag aattctccaa tttacgaatg gttgtatggt cagttattac ttactgatgc
      541 agttttcaac ttatttggaa tatgaattag ccatcaatac tgttatttac cggcaatttt
      601 tgcttgttgt ttattggatt atatttacgg gagcaatagc cagtttagac cggagacttc
      661 gaaattcttt acaggagagg ttagctgttc aaagagaact ccaattggct aatatgatag
      721 attatagcca ccatattgag gggttgtata aagaagttag gagcttccga catgattata
      781 gtaacatgtt aacgaccttg aaactaggga ttgataatga agatatatct attgtcaaat
      841 cagtttatca gtctatcttt aaagaatcta atacacattt ccgacatcct aagtataata
      901 tagaccggtt ggttaatgta gataacccag ccttaaaaag tcttttagca gccaagcttt
      961 cacaagcgat agacaatcat attatggtat ctatagaagt accagacagt attaaacctc
     1021 aagggatgga actgattgat tttattacca ttgtatctgt tttatgggat aacgctattg
     1081 aggcatcttt agagacagac gatcctaaga tagccatgtc ttattttaca ttaaaacata
     1141 aacaaatttt tatcatagaa aattcaacta aggaaaaggc aatagatacg tcaacgatat
     1201 tcaattatga gataagtagt aaggggcgga atagaggaat aggtctttac aatgtcaggg
     1261 gagttatcaa tcgttatcca aatatttcta ttgcaacaag tagccaagat tttagtttct
     1321 gccaaacttt cgaaatcata ttatagtttt aaaaagttca atttatctaa gtttattatt
     1381 ttcttaatac catcaatcca accaccacca gtgatattat ttagttgttc cggcgataat
     1441 tcctgaagct gtagttccat ctcgattagt tttggctagt tctcgtaagt ggtttaataa
     1501 atattttgaa ccatagaact ttgcgattga agcaagtgca gttactccac aatccttagt
     1561 atcaacctga cgcaccaatg cttttttgta cgtcaacatt gtgtaattcc tttcttcgtt
     1621 atcgtattaa tattttgcca taaaaatata tacttattaa aaatagctta aacagctatt
     1681 gcaacgcgtc caatggtttc tataaggata taaaggtaga tagtactttt ctgttttgag
     1741 gcgattcaac gacatttcag ggaataaacg tttctatttt tatttgaatg ctatattaaa
     1801 tgtgtcaagt tgcaacagac aaaaatttta tataggagat attcaaaata aatgtaaaat
     1861 caattgaaca attagaagtt atggataatg aagcattatc acatgttgaa ggtggccttg
     1921 gtcatagatg ggtatacagt aagaaccggt aaacatactt atacaagtgc atggcatctt
     1981 tctgaaaata ctgctgcagc aaatgcaaga aatcgtatgg gcctttaccc tggaagcgtt
     2041 tgtcgtgtct atcttgtata attgattttt tatttaagcg atagggaatc ttccttgccg
     2101 cttaagtttg tatagggaga gacaatacta tgttattaca gctagataat atcactaaaa
     2161 gctacggaaa aaaaatagta cttaaccaga tttcctacca gtttactcct ggtttgtatg
     2221 gactcttagg agcaaatggt actggcaaaa caaccttatt aaatttgatg agccacttta
     2281 cattcgcaga tagtgggaat atttattgga atggacagga gcagtcggaa gaattttatc
     2341 gtcacattgg ttttttaccg caacattttc gctattatga tcaatttact ggcattgctt
     2401 tcttaaatta tatcgctacc ttaaaaggtg ttgataaaaa gaaagctaaa ccagaaattc
     2461 ctagattact agagcttgtt ggtttaggtg atgtaggtaa aaagaagatt tcatcatatt
     2521 ccggtggtat gaagcaacgt ttaggcattg ctcaagcact tatcaatgac cctgaaatac
     2581 ttattctgga tgaaccaact gttggacttg atcctaagga acgggttaaa tttcgccata
     2641 ttttgagtca gttatccacc aataaaatta tcattttatc aacgcatatt gtgtcggatg
     2701 tggaagctgt tgccaaagaa attatagtat taaaaaatgg taaatttatc gaacagggca
     2761 acaccgcaca gttgttgaaa actattgagg gaaaagtgtg ggaaataacg actgaaccag
     2821 atttatctca gatacccaat attgctattg ttaatgaaaa agtcatttca gatagtctgg
     2881 tatttcgtgt agtgagtgat atttgtccga gtgattcggc acagttggtt gttccgactc
     2941 tagaagattt ctacatctat cattttagag gggagtaagt aagatgatat caatttttcc
     3001 ttttgaatta aaaaaacttc tacaacgtaa ggcagcattg ggggctttta ttatcttcct
     3061 tcttgctatt tttggtctat tttaccagca tttttttgtt gggcaaatta gtggctattc
     3121 tgcagataaa atacatggtc gagaagcggt tgcaattaat cgtagaatag ctgaaaaata
     3181 ttcaggggat ttatctgaag aacgagtaac aaatattatt aaaaactatg ttaatccgcc
     3241 agcaggacgc ccccctatat ttgatgtttt tagttattat gtggcagaca cttttgatcg
     3301 tgatttttta atttactata accaattgaa agatgataaa acaattgatt ttaataaaat
     3361 aaatattaaa gatcaaaaac agttgggttc tgcgctacca ctaaaacaac taaagctagg
     3421 taactttgct ccttggaatc aactttttga agtaagtaat agtacttata ttttaattgt
     3481 cttatttact ctattcttat cagctcctct tttttcagga gatagggcta agaagattaa
     3541 tcctatacta ctgacgactc ggtatggacg tggtcagttg acctgggcta agttagctag
     3601 tacaatgacc atcggtattt ccacatttat cctagcatat gccctcattc tgacggtatt
     3661 tgcagattat tttggtttta gtggttggga gacaagtgtt caactgaatt tatactggac
     3721 atcacctttg gctaatatta tgggatttcc tgtacctatg acaatatcaa ctgcttttgt
     3781 tcatattatt gtcttacaat ttgttggact cctttttata gccagtctta atttctttat
     3841 attaagtatc acaaattcgc cattaacatc ttttgctgtt tcaacgcttg ccttttttgc
     3901 tcctagcttt ttaatggcta tgtttaataa aggtgtgata aacaagttat taactatttt
     3961 ctcagtatca acgacagaca cttcagcttt acttttaaaa ttatcacgta atggtagtca
     4021 tggctttttc tttgatagtt tttctgctaa tgaaatctgt ctgattagtc tacgtatgtt
     4081 aattgcagtg ttattgatag cttataccta tcgtttaatg cgacgaagaa gtttatagtt
     4141 cccactggtt atctgaacaa gaaattattc taagctgatt ttcttttgcg aaatcagctt
     4201 ttttatttcc taatatggtt gataaggtat taacaataat tatcagatac catttaaggc
     4261 gtttcaatga cttttgggga taattgagat attcttctga agagattggt atcatagtaa
     4321 tgataactta tagaaagtaa tgttaaggat gaagagaagt cagtgcatgg ataataagca
     4381 gcataataca tcaatgatta gcttgctaca atatcttttt tccatactgg tcattttagt
     4441 tcatagtgga aggttattta gtcaggatgt cattcatttt acctttaaaa gtttcctagg
     4501 tcggatggct gttccttatt ttttgatttg tactgctttc tttttgagag gtcgtataca
     4561 gcaaggtttg tgtaatcact cctactttcg taaactgatt aaaaagtata gcatgtggac
     4621 tatcatttat ttgccttatg gttatttctt ttttgaatct ctaaatatag cgaagattta
     4681 tttgctgccg ggttttattg tggcattttt gtatttaggt atgtcacata ctctttggta
     4741 catccctgct gttattctag gttggttcat tattcaagga ttattgaagt atgtaggaac
     4801 tagggggaca tttatcacag ttgttgtact ctattgtatt ggagcagtag agacctattc
     4861 tgtctttgta cagtcaacaa aattttatcc cctgatgtcc acttatatgt caatttttca
     4921 gacaacacga aacggattat tttacactcc agtttatctt ctagcaggat atttactcta
     4981 tgattatttt aatactgacc tatttaccaa atcgcgtggt cttaagtata ttctcttcct
     5041 tttacttcta gctttagaga atgttcttat ttattttaat caagggttgg ataaaaattt
     5101 cttcttatta gctcctttat gtgcagtatt cttatttaac tggagtatac gtacatcact
     5161 gtttaagggc tatcgtttaa gtcctttaaa acagttgagt gtctattatt tcttccttca
     5221 ccctcttttt gttggcatag tgtcttactg tttaaaatca acttccttag ttgcacacca
     5281 tcaaggaaaa gtgatttttg tggtgacctt ggcattaaca catgcttgtg ctctactcat
     5341 aataaaggtt gttaataaga taccctatca aaaaattatt ccattatctc tttcgaaata
     5401 gtgattcata taagagaaag tttaaagtta tagtgatgta aaggttgtgt aaactattag
     5461 gatacatgac ctttttattg ttctgttttt aaatattaat tatatagaat agtccacaca
     5521 tagagtagaa tagtccattt ttaaaccgtt ttcatttgtt agaatgatag taaaggttat
     5581 cttattcaaa gtattgataa aactttcaga actaatttag ggtaatgtaa taaataggag
     5641 gagaaagatg aagcggaaat tcctaatcgg cagtcacgga aaacttgcga gtgggttgca
     5701 gagttctatt gatattctga caggcaaggg acaagagatt cagacaatag atgcttacat
     5761 tgatgatagt gactatacca agtctattgt agaatttatt gatgagattg caccagatga
     5821 acaggggctt atttttacag atttgttagg tggcagcgtt aatcagaaaa tggcgactgc
     5881 tgtaatgaat agtgggaaaa ataatgtttt tttaataacg aacagcaatt tagcgacctt
     5941 gttgtcatta ctatttttga aacctgaaga ggaattaact aaagaagaaa ttgtaaccgt
     6001 tatcaatgaa tctcaagtgc agctggtaga tctcagcttt aaagcgtgtt cagaagatga
     6061 ctttttcgat taataattag gaggaaaaaa atgattactc aaattcgtgt tgacgaccgt
     6121 ttgattcacg ggcaagtggc agttgtatgg actaaagaat taaatgcacc acttttagtg
     6181 gtggctaatg atgaggctgc caaaaatgaa atcacacaga tgactcttaa aatggctgtg
     6241 cctaatggca tgaaattatt aatccgttca gttgaagaat caatagctct atttaaagat
     6301 ccacgcgcaa ctgacaagcg catttttgtg attgtcaatt ctgttaaaga tgcctgtaca
     6361 attgctaaaa atataaccga tttagaagca gtaaacgtcg ctaatgtagg acgctttgat
     6421 aagtctgatc cagctacaaa agtcaaacta acatcaagtc tcttattaaa tccagaagag
     6481 ttggaagccg caaaagaatt agccagctta ccagatttag acgtctttaa ccaagtattg
     6541 ccttcaaata ctaaggttaa cttgagtcag ctagtcaaac aaagtgaggt atgacatatg
     6601 ataatgccag cgacgatggc agctttagct gtgttgattt gttttggggg aaattactta
     6661 acaggtcaaa gtatgatgga gagaccactt gtagttggtc ttgtaacagg aatgctacta
     6721 ggtgatatta aggttggtat cttaatgggt gcttccctag aagcgctatt tttgggaaac
     6781 gttaatattg ggggtgttat tgccgcagag ccagtaacag caacagcgat ggcaacaact
     6841 tttaccatta tttcaaacat tgatcaaaaa gcagcaatga ctctagctgt tccgattgga
     6901 atgttggcag catttgttgt tatgtttttg aaaaacgtct tcatgaatat ctttgcaccg
     6961 atggtggata aagccgcagc cgcaaaccat caaggtaaac ttgtgatgct tcactatggt
     7021 acatggatta tttactatct cattatcgct tctatttctt tcattggtat tttagtaggt
     7081 agtgggcctg ttaatagttt tgtccatcat attcctcaaa acctgatgaa tggcttgagt
     7141 gcggcgggag gcctccttcc agctgttggt tttgccatgc tgatgaaatt attatggaca
     7201 aataaattag ctgtttttta cttgcttggt tttgttttaa cagcttattt aaaattacca
     7261 gcggttgcag ttgctgccct aggagcagtt atttgcgtca ttagttcaca acgtgatata
     7321 gaattagacg caattactag aggtgcgatc agtaaacaga caacctttga ctctaaagaa
     7381 tcagaagagg aggacttctt cgcatgaaat cacaggataa tttgactaaa gaagaccgta
     7441 aaatgcttcg ctcagtcttc tggcgctcat ggacgatgaa tgccagtcgt actggggcaa
     7501 cacaatacca cgcagtagga gttatttata ctttactacc agtcatcaat cgtttttata
     7561 agactgataa ggataaagca gaggcactgg tccgtcatac aacatggttt aatgccacca
     7621 tgcacattaa taattttatt atgggtcttg ttgcatctat ggaaaagaaa aacagtgaag
     7681 atcctgattt tgacgctagt gctatcaccg cagttaaggc ttctttgatg ggacctatat
     7741 ctggtgttgg tgattcattc ttttggggaa ttctaagggt tattgcggca ggtatcggta
     7801 tctccttagc aagtacaggc tcagccatgg gagctgttgt ctttctactc ttgtataaca
     7861 ttccagcttt tctgattcat tattatagtc tctatggagg ttactctgta ggtgcagggt
     7921 ttatcaaaaa attgtatgaa tcaggtggta ttaaaattgt tactaagact tcaagtatgc
     7981 ttggtttaat gatggtaggc tcaatgactg cttcaaatgt taaattcaag acaatcttaa
     8041 cggtagcagc taaaggagct aaggaagcag cttccataca aagttacctt gaccaattat
     8101 ttgttggcgt agtgccactg ttggtaacga tattagcctt ctggcttcta cgtaaaaaag
     8161 tcaatatcaa ctggatcatg tttggtatta tggtgttagg tattgtactt ggtctcttag
     8221 gtatttgcta ataatctcct agcattaaag gtaagtggaa caagacgata cttgtttagc
     8281 ttatcttttt ttcttttgaa agttattgta tgaaaggagt tgactatctt tattggatag
     8341 ctatttgcta taataaaagg agatggaaat gagaggatat agaatggaag aacgctttaa
     8401 gaaacgacta caagatgata tctctaaaca tttttcccgc cagtctttaa ttttatcctt
     8461 gttattgatt gcacttttcg tccttttttc tttagcgcct cagcagatag gcctttataa
     8521 agatgttaat tcagtttcct atagttataa gcaattgatt caaaaacatg ataccttatt
     8581 agatgactta ggtaaaaata gtttgaaacc atttgtctca ggacacttag gaagtgctga
     8641 tttgagtaag caatattatc atttaaggaa tcatttacaa agtcaaacag aacttttagt
     8701 attctcacct aa
//
LOCUS       DATROY010000075         8602 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.076, whole genome shotgun sequence.
ACCESSION   DATROY010000075 DATROY010000000
VERSION     DATROY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            794..1918
                     /gene="mutY"
                     /locus_tag="VBD11_001367"
     CDS             794..1918
                     /gene="mutY"
                     /locus_tag="VBD11_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6954316.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            2095..2688
                     /locus_tag="VBD11_001368"
     CDS             2095..2688
                     /locus_tag="VBD11_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954317.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2734..3048)
                     /gene="trxA"
                     /locus_tag="VBD11_001369"
     CDS             complement(2734..3048)
                     /gene="trxA"
                     /locus_tag="VBD11_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6954318.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(3129..3629)
                     /locus_tag="VBD11_001370"
     CDS             complement(3129..3629)
                     /locus_tag="VBD11_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6954319.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(3633..5972)
                     /locus_tag="VBD11_001371"
     CDS             complement(3633..5972)
                     /locus_tag="VBD11_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6954320.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(6057..6599)
                     /locus_tag="VBD11_001372"
     CDS             complement(6057..6599)
                     /locus_tag="VBD11_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6954321.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(6602..6913)
                     /locus_tag="VBD11_001373"
     CDS             complement(6602..6913)
                     /locus_tag="VBD11_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954322.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            7024..7917
                     /locus_tag="VBD11_001374"
     CDS             7024..7917
                     /locus_tag="VBD11_001374"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6954323.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            7933..8526
                     /gene="lepB"
                     /locus_tag="VBD11_001375"
     CDS             7933..8526
                     /gene="lepB"
                     /locus_tag="VBD11_001375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6954324.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
BASE COUNT         2838 a         1658 c         1358 g         2748 t
ORIGIN      
        1 ttggtttaag ttttcgttgg agaaaaattc agtgtctccg attttatatt tttaaatatt
       61 aaaatactat ttagggagtc cctctttatg ttttattacc tttggtaaca ccaaaacata
      121 cacgaacttt tattcatatg tgttaccatt ttacatcttt catattgaga tatttgatta
      181 gcaatacctc cttgactaac aaaaattact aacacatcta ggggtatacc ctagtttata
      241 tttgttcatg attacctcaa gcgagcatgc gctaatggta atgaactatt accacattgt
      301 ctgtcttcct taaattatat aaggacttat gtgtcaccgt tactgtagcg acctacatct
      361 aaacctaaca taagtgcccc tccaactatt tctaggaaat gaagaaaact tcaaatcctt
      421 gtgactgttt gcaacatcaa tgcattgata acaattttag tgtatcgcca ttcttatagc
      481 gacccatatt aaaaattaac ataagcgcta cctccattaa ctaaagttag cttgcctacc
      541 aatctttctt ttggagacta gtctccattc ttatacctag agatattgta atagatcatg
      601 gataacctcc ttgatttaaa ctttatcttt ataatttaat tatagtgtaa acgcttcctt
      661 aagtcaagaa aaaaatattg atgcaacatt tttcttcatc attaagtacg atctagtcaa
      721 catacctctt tgtcaaatcc tctttctttg atacaattaa tctatgatag atttaaaaga
      781 ctatggaatt gagatgtggc ctgaagatag aatcgcttct tttcgtcgca cacttttagg
      841 gtggtatgac caagaaaaac gtgatctccc ttggcgaaga actactaatc catactatat
      901 ttgggtttct gaaatcatgt tgcaacaaac acaagttaat acggtaatcc cttattataa
      961 acgttttcta gagtggtttc ctcaaataaa agatttggca gacgctcctg aagaacaatt
     1021 actaaaggca tgggagggac taggctatta ttctcgggtg cgtaatatgc aaaaggctgc
     1081 acagcaagtg atggttgatt ttggtggcat atttcctcac acttatgatg atattgcttc
     1141 attaaaaggg attggtcctt atactgcagg tgctatcgcc agcatctcat tcaacttacc
     1201 agagcctgct gttgatggca atgtgatgcg tgtcatggct cgtttgtttg aagttaatta
     1261 tgacatcggt gaccctaaaa atcgtaaaat ttttcaagct attatggaaa tactaattga
     1321 ccctgacaga cccggtgatt ttaaccaagc acttatggat ctgggtacag acatcgaatc
     1381 cgctaagaca cctagacctg atgaaagccc tattcgtttc tttaatgctg cttatttgaa
     1441 tggtacttat agtaaatatc ccataaaaaa tcctaagaaa aagcctaaac caatgcgaat
     1501 tcaggctttt gtcattcgta accaaaatgg tcaatatcta ctggagaaaa atactaaagg
     1561 gagacttcta gggggttttt ggtcatttcc tattatcgaa acgtcacctc tttctcaaca
     1621 attagattta tttgatgaca atcaatccaa ccccataatc tggcaaaccc aaaatgaaac
     1681 tttccaaaga gaatatcaat taaagcccca atggactgat aatcactttc caaatattaa
     1741 gcacactttt agtcaccaga agtggacaat agaactcata gagggggtgg tcaaagcaac
     1801 agatttacct aatgctcctc acctaaagtg ggtagcaatt gaggactttt ctctgtatcc
     1861 ctttgcaaca ccacaaaaga aaatgctaga gacctactta aaacagaaaa atgcttaatc
     1921 acaagtgaac tatgacggtt cactttttgt ttacaattcc tttacgccgc tgtaaagttt
     1981 tcctcagatt atcttttaat accctataaa tagtggtcta tccctaatgt caaatacatt
     2041 tgatagaccc gttagcttat catccttata ctaatattat gaaaggagat atttatggaa
     2101 attggtcagc aaattattcg ctaccgtaaa caacaagcgc tctcgcaaga agaattagcc
     2161 gagaaagtct atgtaagccg tcagagcata tctaactggg aaaatgacaa aacttaccca
     2221 gacatccata gcctcttact cctaagtcaa atttttcaag tgagcttaga ccaactaatt
     2281 aaaggagaca ttgagaaaat gaaatatacc atcactcagg ttgataaaaa gaattttgaa
     2341 cgagatacca aagtaatggt gacattgatg atactattaa tgatttcaag ttatccatta
     2401 gtctatttct tagagtggct aggacttggc atttttgttt tattatcgat aattactatg
     2461 acttatgcta atcgtgtgga acgttttaag aaaaaatatg atgtgcaaac ttataaggaa
     2521 atcctagctg tttcaagtgg aaaattatta gacgaaatcg aaaaacggga agaaagagca
     2581 aaacttcctt atcagaaacc ccttattgtc actgtatttt tcctcattac tgttgccacc
     2641 ttttttgcaa gtcgttttat tttcacatgg ttgttccatt taagttagta gataataaaa
     2701 taaagctcag cgtgacactg ggctttattt tatttaactc aattcagcaa taatagcttt
     2761 cagttggtct ttagtatgaa cacctgcaac ttgtttaacc acttctccgt cttttttaaa
     2821 catcaatgtt ggaattgaca taataccaaa ctgacgggca gtttccggat tctcatcaac
     2881 atccattttc aaaattttga gttcatcttc atcgatttct tgagataact gttctaagat
     2941 tggtgcttgc atacgacatg gaccacacca agttgcccaa aaatcaatga gtactaaacc
     3001 ttctttagtt tcttctacaa atgtagcatc tgttacttct aatgccatct tttttctcca
     3061 tttcatctta atgataagcc tattgtacaa gaatttagtt gtttttgaaa ctatttgcta
     3121 cgaactcgct aaataaataa acaaagccct aaaatcaagc caataaggta acctacaata
     3181 acatccttag gatagtgaat acctgctatc acacggcaaa ttgccaatag aaccgataat
     3241 attaaacaaa ctattccaaa gtaaacataa tatcgtaaca aacacatact aatcatcgta
     3301 gctgaaaaaa catgtctgct aggcattgac cttccctcag tatccttata aattaaaggt
     3361 ttaatattcc atttttcata tggtcgtgga aaatcaaggc gtttcctaat gtaagacaaa
     3421 gctataaagc caacagcagg gataattaat agccttaacc ataattgaaa tgtcatccct
     3481 tgccaaagag taaatatcag aaaagatgga tagagtatgt acatcattcc tgtgactatt
     3541 tttagtaaaa aatttaaaag aattattaac tgtggggatt ttctaaaagg ttgagatagt
     3601 ttggcataaa attgttcata tgattccata atttacccta acgttacaat tgtggcgcca
     3661 gatccccctg cattttgtgg ggcataagca aaatgcttaa cgtgcttatt acgacgaaga
     3721 tattttgtca ctccctcacg gataacgcct gtaccaatac catgaatgat atcaacttgt
     3781 cccatattgt taagcaatgc ttgatcaata aaattatcta actcttgcat agcttcttcg
     3841 tatcttttac ctctaagatc aagtcgagct cttggtccag aactatcagc ctttttaacc
     3901 acattaatct gtttactttt gactttctgt tcttcttgga ctctaacaag ggtaaattca
     3961 tcttgtgtta atgtcatttt gataattccc acttgtgctt cccaacgtcc atcttttaat
     4021 tgactagtta aggtacctcg ctgtccatag ctagtcacta taatatcatc accaattcta
     4081 ggagcacgag ctgctttaat ttttttagcc ttatttaaga ctttattttt tgataaatca
     4141 acttgaggtg ctaatttttt tatttgtgcc ttagcatcta taatttcgtg aggttttaat
     4201 tggctcttat cattgagttt ttttaagata gtatcactct cattcaaagc catatctaca
     4261 atttcttgag cttcttgata gattttttct aactctttat cgcgctcatg tgaaaattca
     4321 ttatagagtt tcttaaccgc acgattgaat ttgaggtttt cttgttcaac ttctttaata
     4381 tgatccagtc ttctacgtgt ctcaagtgtc tgtgcctcta actgttcaat aatacggtta
     4441 acatctgagt cagaatctgt catctgctta gcttgtttaa caataaatgg agctaaacca
     4501 aggcgagaag caatttcaaa tgcatttgat cgtccaggaa ctccttgcat aaagcgatac
     4561 gtagggctaa gcgtttcggc atcaaattcc atgctcgcat tctctacaaa atttgtctca
     4621 atcccataag cttttaattc tggatagtgc gtggtcgcca tcgttttgat attacttaac
     4681 ctaaggtgtt ctaaaatagc catagccaaa ctagcacctt cttgaggatc cgttcctgct
     4741 cccagttcat caaagaggac taaactattg tggtcagcct cgtttaaaat actgactatg
     4801 tgcgtcatat gactagaaaa agttgataga ctttgttcaa tagattgctc atcgccaata
     4861 tctgcaaaga tattgttaaa tactgcaatt ttactacctt tatccgctaa tactggcaaa
     4921 ccagactgtc ccattaattg tgctaaaccg agtgttttta gcataatcgt cttaccacca
     4981 gtattgggac cagtgatgac aattgcagtt aaatcttgat caaaatgtaa gtcattagcc
     5041 acagggttac ttaacagagg atgacgaaca ttgattaatg ctaacgtgct gtcattagaa
     5101 atctcaggta tcgtcgcctt attatcagac ataaaaagat atttagccct tacaaaatca
     5161 agatgcccaa gaatccatgc attatttcta atagtggcga catggggtct taacaagtct
     5221 gaaaatgcgt gtaaaatacg actttcttca tgacgttcgt cagctctaag ctgcgttatc
     5281 tcttcgttta gtgtaactac agcacgaggc tcaatataaa cagtacttcc tgaagaagag
     5341 atgtcatgaa ccacaccaga aatacgattc cgataagtat tttttactgg taggacactt
     5401 cgtccactac gactagcgat tagattctct gataaaagct ctgctttttc cttaagcata
     5461 tcctgtaaaa tctgacgaac ccgtcgttca ctgtttgtta attgacgacg gatacgctct
     5521 aattctggac tcgcaaaatg ttctaaaaaa ccaccatcat tgatagcttg aaaagaccct
     5581 tgcagattag ggaattgttc caaattttca aacaaacgat ccaaagattg gaaagaaaca
     5641 ttatccaaat cagaataaaa gtgaatcata tccgaagaac tctgtaaaac ttttttgata
     5701 gccaaaagtt cttgaatatt aagatcagct gaaagctcta agcgttttaa actctcagag
     5761 attgaactta ggctgactat gccaaatgag tgatgttcaa caaaaatctg ttccatgtca
     5821 gaaatttcat taaaactttt ttctatttta ggagcctctg tcatcggctc cagctctgat
     5881 aattcttctt gtgattgttc tgtcttgaga taaggtaata tcaattcctt aactttgtta
     5941 aattctaact gttctaaaat cttgttattc atactcttca ttatacctaa atttatcaca
     6001 aaatgacaac gttaaaaagg ttggaactct tactccaacc ttactaaaca ttatattcaa
     6061 agacctaccg cccaagtcct taccaaaaac tgtgagaaga atggtaagtc gttaattaaa
     6121 aatctaacaa acagactatt ccataacttt tcttgaacaa atgacatagg gactgttgcc
     6181 agaatagtca aacacatact cataaataag agacaaacca gaacggctaa aaaaccacta
     6241 ataatattat tttgcaagct atcaaaatgt tcaactggtg ctaaatgtag taaaacacct
     6301 aacaagcgac ctactaagta agataaactg tagataaaaa caaaggcaac acctgcataa
     6361 taaacatgac tcaattgaaa tttgctaata tctttaaaaa agtaaacttc tactccttgt
     6421 acaggattag aatacggtac ccataacgta atctgactag ctaattcttg gtaaaattga
     6481 ctagcaatca tcaaagatac cattgacatt aacacgtaaa agacttgtaa aaaaatacct
     6541 ctactgtagc caatataaaa atgccaaata acaataatca gcaaaagcaa tgatagcatg
     6601 attaatcctc actcactttt tctttaatgt ccgaaatcgt tttggagcgt aacttattga
     6661 gctcatcttc cattttttca atgtcaattt ctcgacttag ttgcactgat aaagcattaa
     6721 ttgccatgag aatagcaatc gtttcattat cagcattagg taatttttct ttaatagctt
     6781 catacttttc tgtagctaca cgctcaatct cttccataaa aaggttgtct ttatctgtcg
     6841 tcaatgtgag aggcttatct ccaaagacaa atttataacg atttttctga ctattattta
     6901 tttttgtttc catactatac ctctgctagc attataccgt aaaatagggc tttcgtaaaa
     6961 ttctacccct tatcattact tgtctattcc cttattcctc ttttttatga tagaatattg
     7021 tctatgaaca caatcgtcat gcaagcagat aaaaaattgc aagaaaagat aagaaccgat
     7081 ttagctcaac atcatataag taataacaat ccttatgtgg tattttcagc taaaatatca
     7141 ggagctactg ttcttttata tacttctgga aaacttgtct tccagggttc taacgcttca
     7201 catatcgccc aaaagtatgg ttttattgag caaaaagaaa gttgttcctc tgagagtcaa
     7261 gatattccca taattggtac tgatgaagtg ggaaatggct cttactttgg tggactggca
     7321 gtggtagcta gttttgtaac tcctaaggac catgcttacc tcaaaaaatt aggtgttggt
     7381 gattcaaaaa cacttactga tcaaaaaatt aagcaaatcg cgcctctttt ggaaaaagca
     7441 attccacaca aagcattatt gttatcgcct caaaaatata atcaagtggt tagtccaaat
     7501 aacaaacata atgctgtctc cgtaaaagtt gctttacaca atcaagctat cttccttctt
     7561 cttcaagatg gttttgaacc agaaaaaatc gttattgatg cttttactag ctccaaaaac
     7621 tatcaaaatt atttgaaaaa tgaaaagaat caatttaaac aaacgattac tctggaagaa
     7681 aaagctgaaa ataaatatct agctgtagcg gtcagttcta ttattgcacg taatctattc
     7741 ttagaaaacc ttaacaaatt aagcgacgac gtaggatata aacttccaag tggagctggt
     7801 caccaatctg ataaggtcgc tagccaatta ctcaaagctt atggtatctc ttcgcttgaa
     7861 cactgcgcaa aacttcattt tgccaacacc aaaaaagcac aagcattatt aaaataaata
     7921 gcagggaaaa ttatgaaaga atttattaaa gaatggggtg tctttatcct catcctctca
     7981 ctttttttac tatcgcgtat ctttttatgg caattcgtta aagttgacgg acactccatg
     8041 gatccaactt tagctgacaa ggaacagcta gtagttctca aacaaacaaa aatcaatcga
     8101 ttcgatattg tagtggctaa cgaagaagaa ggcggccaaa agaaaaaaat tgttaaacgt
     8161 gtcattggta tgccaggtga tgtcatcaaa tataaaaatg acaccttaac tattaacaat
     8221 aaaaaaacag aagaacctta cctcaaggaa tatactaaat tatttaaaaa ggataaatta
     8281 caggaaaaat attcgtataa cccacttttc caagacctag cacaaagctc taccgctttc
     8341 accactgaca gcaatggcag cagcgaattt actactgtcg tgcctaaagg ccactactat
     8401 cttgttggtg atgaccgaat tgtctctaaa gatagtcgtg ccgtcggttc cttcaaaaaa
     8461 tcaacgattg tgggagaggt taaattccgc ttctggccaa ttcgtcgttt tggaactatc
     8521 aactaaagga aattaaaagt tcacctgaca aaagttgaaa tagcatatta tattgaaaat
     8581 agggagtgac aaatccattt aa
//
LOCUS       DATROY010000076         8501 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.077, whole genome shotgun sequence.
ACCESSION   DATROY010000076 DATROY010000000
VERSION     DATROY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            57..704
                     /locus_tag="VBD11_001376"
     CDS             57..704
                     /locus_tag="VBD11_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6954325.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            complement(744..1433)
                     /locus_tag="VBD11_001377"
     CDS             complement(744..1433)
                     /locus_tag="VBD11_001377"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6954326.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(1443..1712)
                     /locus_tag="VBD11_001378"
     CDS             complement(1443..1712)
                     /locus_tag="VBD11_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954327.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(1712..2266)
                     /locus_tag="VBD11_001379"
     CDS             complement(1712..2266)
                     /locus_tag="VBD11_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6954328.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(2287..3666)
                     /gene="glmU"
                     /locus_tag="VBD11_001380"
     CDS             complement(2287..3666)
                     /gene="glmU"
                     /locus_tag="VBD11_001380"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6954329.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(3910..4323)
                     /locus_tag="VBD11_001381"
     CDS             complement(3910..4323)
                     /locus_tag="VBD11_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6954330.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            complement(4336..4713)
                     /locus_tag="VBD11_001382"
                     /pseudo
     CDS             complement(4336..4713)
                     /locus_tag="VBD11_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(4802..5758)
                     /locus_tag="VBD11_001383"
     CDS             complement(4802..5758)
                     /locus_tag="VBD11_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6954331.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(5755..6207)
                     /locus_tag="VBD11_001384"
     CDS             complement(5755..6207)
                     /locus_tag="VBD11_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO6954332.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            complement(6204..6449)
                     /locus_tag="VBD11_001385"
                     /pseudo
     CDS             complement(6204..6449)
                     /locus_tag="VBD11_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(6446..7144)
                     /locus_tag="VBD11_001386"
     CDS             complement(6446..7144)
                     /locus_tag="VBD11_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6954333.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(7137..7373)
                     /locus_tag="VBD11_001387"
     CDS             complement(7137..7373)
                     /locus_tag="VBD11_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6954334.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(7463..7711)
                     /locus_tag="VBD11_001388"
     CDS             complement(7463..7711)
                     /locus_tag="VBD11_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954335.1"
                     /translation="MEKITNDFEKVIAVLNRLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            complement(7909..8409)
                     /locus_tag="VBD11_001389"
     CDS             complement(7909..8409)
                     /locus_tag="VBD11_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954336.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
BASE COUNT         2670 a         1818 c         1163 g         2850 t
ORIGIN      
        1 ttttgatata atggtcttag aaaaatagat tttaagaagt ataagaaaga ctgaatatga
       61 ctccaaataa agaagattac ctaaaatgca ttcatgaatt aggtgaaaaa aacaacaaaa
      121 tttccaataa gaaaatcgct gaaatgatgc aagtctcggc tcctgccgtg tcagaaatga
      181 ttaaaaaaat gataagtgaa caactgattg ttaaagataa agatttagga tattatctta
      241 ccaaacaagg tttattagtt gtttctgacc tttaccgtaa acatcgtcta gtagaggtat
      301 ttttagttaa ccatttgcac tatactgctg atgatattca tgaagaagcg gaagttttag
      361 aacatactgt atcaacaact tttgttgatc aattagaaaa gttgttagac tttccacaat
      421 tttgtccaca cggtgggact attcctaaaa agggagagtt tttagtagaa attaaccaaa
      481 tgacattgga ccaaattagt cagcttggca catatgttat cagtcgtgtt catgatgatt
      541 ttcagctact gaaataccta gaacaacacc gtttgcatat caacgatacc attgaattga
      601 cacaaattga cccttatgca aaaacttatc acatcaccta taacgatgaa aatctaacaa
      661 tccctgagcg tatcgcaagt caaatatatg tatctgctac ataaataaaa aagtccgtgt
      721 attcattaga tacacgggct tttttataat gctttcaaaa atgccattaa tacttgggca
      781 gagcgtttac cagcttcaat gataaattca tcaaatgtga tattggcatc atgagctgca
      841 gtatcactca tagcacgtac caccacaaag ggctttcctg tagcttgagc agcttgggca
      901 attgctgcac cttccatctc gacagctaaa acctgtggaa aatgcccctt aataacatca
      961 atcttttcct ggccagcaat aaaactatcc ccagttgcaa ttaaaccaac tttactagtc
     1021 atttcttctt tagataaaac tgcttcaaaa gtggaaacaa aagtcttatc cgaatgaaaa
     1081 taaagtggct gcattgacat ttgtccatag tcataaccaa aggcggttaa atctacatcg
     1141 tgataaacta atgtatctgc gacaacgacg tctcctacat tcaatcctgt tgctactgca
     1201 ccagctgatc ctgtattaat aatagcatca actttaaagc tttctactaa aatagcaaca
     1261 ctcatcgctg acatgacttt cccaacacca gactgtacta aaacaagctc atgttcacca
     1321 taacgtccac tgtaatatac atttgataat actgtctctt gagatttatc ctcaagattt
     1381 tccacaagga gttttaactc ttcctccatg gctgcaataa ttccaatttt catgatattt
     1441 ccttataatt taaaaattgc ataaaacaag agagccaaca aaataagcaa aatgaataaa
     1501 actaaattca acttacgttg aaattcattt cgttttgcat tctcaatccg gcgactcttg
     1561 tagatatgtt taccgttctc gtcagttagt tgctccatgc catcttctgt agaaataatt
     1621 ttagtttctt gatctaagat tgtttgacct gaaacttttt cacctcgatt tgaacgttca
     1681 atcatatcat ctgttaacaa tggttttccc attaagaatg acctcccatt tttaaaccgt
     1741 aatattgtaa agcaatgatc gttttcgcat cttgaatcat gcctttttct accatttgca
     1801 tacaatcctc ataacttaac tctagaagct caaggacctc atcatcatct tgtggcctag
     1861 gattttcaac tttttgtaaa tcagtagcta gatagaggac gattttctca ttacaaaaac
     1921 caatagcagt gtagaaacta taaagaattt caagattccc tgtgtagcca gtctcttctt
     1981 ctaattcccg aagggcagct tcttccttag aacctgactc acccgtctca agtttccctg
     2041 ctggaatttc gtaagaaata ccttcaatag ccttacgata ctgtttcact aaaacaattt
     2101 tatgctcagg agttaccgcc aaagtagcaa cagctcctcc atgaaaaaca agttcacgtt
     2161 tggattgtcc caatccattt ggcaactcta catcatcaac agctactttg ataatttgtc
     2221 catcaaaaac agtttgacga ttaattgttt tttcttcaaa atccataaga acctcccaaa
     2281 taagtgttac ttttgtgaag gatgatgtgg ttttttattg gcatatcctt ctttattgac
     2341 ttgacgtcct cttccaatag ctatgctatc aattggaaca ttatctgtga tggtagatcc
     2401 agctgctgtt aaagcattat caccaatttc aagtggtgca atcagagtag aattacttcc
     2461 gataaagaca ttacttccga tttctgtttt aaatttattt tgtccatcat aattgacagt
     2521 aatcgtccct gcgccaaagt taacatcaca acctacctct gcattgccga tataagttaa
     2581 atgacctgcc ttcgtattct cacctatttg agaacccttt acttcaacga aattaccaat
     2641 atgaacacct tttgcaagac ttgttcctgg tctaatatgc gcataagggc ctacggttac
     2701 tccatcagaa ataatggatt cttcaaccat cgaattagtg atggtaacat catttccgac
     2761 ttgagcatct actaaataac taccattggt taataaagta cctttaccaa tcttcgtctg
     2821 tccttttaaa gtcacattag gctcaattac agattcttct ccaatctcaa catcaatatc
     2881 aatataagcg gaatcaggat taaccactgt gaccccatta accatatgtt gacgtgcaat
     2941 tcggtgacgc atcacttttt cagcagttgc aagtgctaca cgatcattaa ctcctagact
     3001 ttcgtcaaag tcacgaagtt tataggcacc taccttttta cccgcttctt tgaaaattcc
     3061 aataacatca gttagataat actctccttg agcgttattt gtattaatgt ctttaagtgc
     3121 ttcaaataga ctttgattat caaaaacata agttccagta ttaatctctt taacctgctg
     3181 ctcaaagtca ttagcatcct tttgttctac aattttagta acttcatcat ctgaattacg
     3241 gataatacga ccatatccaa atggatttgc agcatcggct gttaagattg ttgcaacgtt
     3301 tttatgattg acatgaaaat caattaagtt ttttaaactt tctccagtaa tcaggggtgt
     3361 atcacctgca ataactagtg tatgtccttt tgatgtcgct aattcttctt ccgccatcat
     3421 tacagcatgg ccagttccta gctgttctgt ttgcattaca aactctgatt tgtcacctaa
     3481 aacatcacgt acaagctccg ccttatgtcc aataacagta acaattttac ttggctcaat
     3541 agcttgcaca ctacggaaaa catgctcaag catggtaata ccagatactt tatgcataac
     3601 ttttggtaaa tcagatttca tgcgagttcc cttacccgca gctaaaataa tggcataatt
     3661 tgacatatta attcctctat ctctgaatat aattcaacct attatatcat aatgccttca
     3721 acttatcact aatacccctt taaatcccta ttacaataga ctttagctaa aatagcttac
     3781 ttcaattagg aataaaatag ctaccttttg gtagctattc aatacatgaa atccgttaat
     3841 tttttcttct ctgtttgcaa tcagtatttt tataaatcta tcattgcaat gctcagatgg
     3901 taaaaacgat tagtttaact ctgttgaaat ttgccacatc acaccaaatt ggtctactaa
     3961 gtttgcgtaa gcaggattcc agaaagtttc ttgtaactct aactgaactt cttgtgcact
     4021 cttctttaat ttttcataaa tttcacgcgc ctcctctacg ctacccacaa taatagcggc
     4081 tgtaacattt ttaccagctt gatacacgaa atctggattt tcatccgaga tttgaagacg
     4141 aatgccatca aaaataagct gagcattaat gattaaatcc ttgcggtctt caagacagtt
     4201 ggggtccatc tcttcccatg tcatcatatt cactaaatcc gcttgaaaga catctttata
     4261 aaaatcaacc gcttgtcgcc catttccatt tgttacgatg tatgtttcta gtgctttaac
     4321 cataagttct cctttttatt ttacttcacg gattgcaaag taattattgt cattatcgga
     4381 aaaattaaaa actctcatac tccccatatc aacaatagga tttgtcataa cctcatttgc
     4441 tgttaattct tgatatgtac tgtcaatatc agtagtttca aataaaatag agggagttgc
     4501 taaatctagc tcaggactca tttgagctat aattgctttg tcatgtaaga cgaagctaac
     4561 ttcactatcc aacttcggtg caactatata ggttacataa tctccttgtg tctatttttc
     4621 aacacgctca aaacccacct tgtttttcca aaaatctgct gatgcttcaa cattgctaac
     4681 gtagagcatt acctgtccaa ttgatgaaat cattttatta tctcactttc ggttttatta
     4741 ctaattagta taacatacag tatttaaaat aagtaaaaaa tgactcaaaa gagtcattta
     4801 cttatttcgt taccatatct agaactttca tcaccgttct actatgttct aaagcttgag
     4861 cagcacgctt aaaatctaaa ttgctaataa taccttcaaa aataacaaat tcatcgtgca
     4921 tgcggtgcct atcaccattt aattgataaa catgactttc ttgtccattc atagttaatg
     4981 aaattttagg catagtatta gttggcccta aaatcgctat agacccctta tcaccttgaa
     5041 tagtagattt tacttctgca ctacaatctt ttgctccaat acagactgtt ttaaagtgcc
     5101 catagtctag gactagaact cctgaagtat caataccacg ctctatattt ggtaaatact
     5161 gtgctgttat aggttctcca aaaagtccaa ttactaaatg taaattataa atattaaggt
     5221 ctctcaacgc acctccaccc atttcaggat tgaaagctgg tgctatttcc ccacgtttaa
     5281 aggcatcata acgcgatgaa tattgggaat aattacattc tacaatttta atatctccaa
     5341 ggttgcttaa atgttctttt acaaggtcaa aattaggtaa atattgattt gtaattgctt
     5401 cgagtaatat tagttgccta gtattggcaa tagatacaag ttcttctaat tgacttgctt
     5461 ctaatgtaaa tggcttctca caaatgacat gttttcctgc caagagtgcc tccttagcat
     5521 agtcaaaatg caagtgattc ggcagtccaa tatagactgt gtcaatctcc tcattgtcca
     5581 agacagcctt atattcactc gtagctagag acatgttata ttccttagct agatctttag
     5641 ctgtctccaa acttctaact gttgataaga tagcaactag atcaatacct tcaattttct
     5701 gcaagacagg caaaacttcc ttaacaatca tacctgtccc caaaaccgct aatttcattc
     5761 gttaatctcc tttgggtaaa ccacttggaa ttcctctaaa actatcctag agtcctcact
     5821 aaacattaaa ccaaactcct caaaataagg tttaaagaaa tcaatgtgag cttttctcca
     5881 ccattctaaa gttttatctc cttctccttc tttaaaagca tggtctgcag agacatcttt
     5941 aaaagggaca atatcaactt tagttatttc aataatacag atagcttcat cattgctatc
     6001 caaaataata tcatatgaac caacttgtgg taacccttcc tcatctaatt catataaatc
     6061 ataggcagag gccgttgcag tttttgtccc atctacaact aactttgcca gaagatctgg
     6121 ttctgctcca aattgccatg cgtctatctc atcaccgatt gaaggattca aagatttaaa
     6181 cttattccat aattcttgtg ccttcatatt acctccgaag caattcccac aacaaactta
     6241 actgcgttac tcatttttct ttgtctttta aatccttctt taacatctaa aatataaaca
     6301 gtttgaccaa tccaaacacg taaataaaca gattttatta aaatatcacc tgatattcct
     6361 ttaacttccc gctcagtacc atcagatgct tcaaattccg tacgtaataa ccaagtatta
     6421 ccacaaccaa attcaatcta ttttctcatt tcaaactcca gccaccatca attttgacaa
     6481 tttcaccttg cattgatctc gcttttcctg aagcaagaaa tccagttaat tcagcaactt
     6541 cacttggttt agtccaacga ccaattggcg tctcactagc tacccattct gctaatccac
     6601 caggttcaaa atcactagca gtcatagcag tttgtacagc acctggcgct ataccaaaaa
     6661 tttgaataca atcttttgcg taatccaaag ccaattgacg tgtgaatccc gcaagggcat
     6721 gtttagaact agtatatgca gctccaccgc cacccgctat aaaacttgct attgagcaca
     6781 tattgataat aatacctgac ttcttttcca ccatacgtct aagataatga cgtgttagcc
     6841 taacagtcac gaaaaaatta atatcaaata ggtgttccag ttcttcatca cttacttcta
     6901 gaagtggctt gtaggcatct agaatacctg cagtattaca caaaatatca acagtcggta
     6961 ccattgtgaa tagtggggat aaatcagagg ataaatccaa ctttataaag ttaaaattac
     7021 catttaagtt cggtttatcc gacttatcca caccatatac ttgataccct tgtttcaaaa
     7081 aatattgagc ctgtgcataa ccaattccac tagcacagcc cgtcactaaa acaactttag
     7141 tcattgacct cgatccaatc ctctgctaaa acatcacatg gtgttggtgc ccacatggaa
     7201 aaaccttcac cttcaccagt aacattaata agaaaataag gtgtagcctg tagtactttc
     7261 ccattaactt caagggtatc aaataactga acataatttt cagcgtcccc ccaacctgta
     7321 cgtacatatt ttttctttgc ttttaaacct ggtaaaattt cttcaaatgt catattgttc
     7381 tcctttattg tctttaacca ttgatatatc aaggtttatt gcttatttaa aatcataatt
     7441 taaagtttct tcgcctttta gttcatctaa aggctactat tgttttcata ggataaaata
     7501 gtttctggtc atccattatt agtaaataat atttcgtctt tttcttgttg gtcaagaaca
     7561 ttatttttgt tcttactcct tcaattccca caataaagtt atctgcttgc tccgtcaaca
     7621 aaacaggtcc attttccact aattcataag aaatacttag tcgatttagc acagctataa
     7681 ctttttcaaa atcattcgta attttctcca ttcttccata gagttagcat aactatttgt
     7741 agactagaga acctaaaatt gatacactgt ttttcactca aaaaatttta ccttcataat
     7801 cttcattata cctataacaa ttttctttta caaatttgtt ccccttaaag gaaattgttt
     7861 ttcaaaatag gatgtttata ctattgtatt atcggatgct ttatctcgct agtgcaaaat
     7921 cttctcataa cacatttctt ccttttcaaa ataagttttt gatttaccct taaaagaaaa
     7981 accaaaccta tcgtaaaatt tccttgcgcg tgtattttct gtaaataccc aaagtatcac
     8041 tttattataa cctgcttgtt gtatctctga tagtacatga ttcatcattt gagacccata
     8101 accttttccc caaccttctt taagactatg tatacaaatg agttctgcat aatcaagcat
     8161 accatcttct ctacttttat cccaccaagc catacaatga ggattgctat ctacttccaa
     8221 tatatacccc ttaccaacct ccttgtgtag tagctgttga tacattgtta tggcaggttc
     8281 tatttccgta gttttttgaa tgatatcctc aggtagtatc ttacccaaag cagctttcca
     8341 gctctccgtt tgaatataag ctaaattgac ttcatctcct aatcttgctc tcctgattat
     8401 gcaagccata actaaaatcc tttcaaaaat tattattaac tgcctgataa ctactccaat
     8461 tataccattt tcgtgatatt aatttttatt ttgaacagtt c
//
LOCUS       DATROY010000077         8500 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.078, whole genome shotgun sequence.
ACCESSION   DATROY010000077 DATROY010000000
VERSION     DATROY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            14..694
                     /locus_tag="VBD11_001390"
     CDS             14..694
                     /locus_tag="VBD11_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6954337.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(715..1710)
                     /gene="galE"
                     /locus_tag="VBD11_001391"
     CDS             complement(715..1710)
                     /gene="galE"
                     /locus_tag="VBD11_001391"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6954338.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(1798..3405)
                     /locus_tag="VBD11_001392"
     CDS             complement(1798..3405)
                     /locus_tag="VBD11_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6954339.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(3534..4667)
                     /gene="ugpC"
                     /locus_tag="VBD11_001393"
     CDS             complement(3534..4667)
                     /gene="ugpC"
                     /locus_tag="VBD11_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6954340.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(4768..5619)
                     /locus_tag="VBD11_001394"
     CDS             complement(4768..5619)
                     /locus_tag="VBD11_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6954341.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(5714..6610)
                     /locus_tag="VBD11_001395"
     CDS             complement(5714..6610)
                     /locus_tag="VBD11_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6954342.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(6664..7641)
                     /gene="lacD"
                     /locus_tag="VBD11_001396"
     CDS             complement(6664..7641)
                     /gene="lacD"
                     /locus_tag="VBD11_001396"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6954343.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(7643..>8500)
                     /locus_tag="VBD11_001397"
     CDS             complement(7643..>8500)
                     /locus_tag="VBD11_001397"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6954344.1"
                     /translation="NRVTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRS
                     GLDALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDA
                     ATIITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPN
                     LEELTQLIGKPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPK
                     IEVINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQEL
                     ISQIQVKEV"
BASE COUNT         2633 a         1714 c         1396 g         2757 t
ORIGIN      
        1 gaagggtctt agtatggacg tattaattat cgaagatgat cctatggtgg aatttatcca
       61 ccgcaattac cttgaaaaac tcaattattt tcaaaacatc tattcaactg ctagtcaaac
      121 acaagctatt gcttatctta atgacattaa aatccaactt gttcttttag atattcatat
      181 taaagaaggc aatggtctag aattattaaa gttattacgc aatcaacacc aaaataccga
      241 agtcattgtt atttcagcag ccaacgaggc gcacactgtc aaagaagcat ttcatttagg
      301 tattgtagat tacctgataa aaccatttac atttgaacgc tttgagtcta gtattgaaaa
      361 attcctaaat cattatcata catttgaagc tgataaaatc tatcaagaca acatcgatca
      421 ctttcaaaaa atagattctg gatggctaga gggagaggtt aaacttgatg agaaagggct
      481 ctctgaaatt acctatcagc atattcttga tgccattcaa gagttagagc agcctttcac
      541 tatccaagag ttagcaaaat gcagtcaatt ttctcatgtc tctgttagga aatatattgc
      601 ctatatggaa gaaaaaggcc ttctgacaag tcagcaaatt tatactaagg tcggtcgtcc
      661 ttacaaagtt tataagctta taaaaaaaac ttaggcctgt agacctaagt ttttttaatc
      721 ttcgtagcca tttggatgac tagaatgcca tgcccatgcc gatgaaataa ttttatcaat
      781 attatcaaat ttaggctccc acccaagcat ttgacgagct ttctctgagg aagcaataag
      841 cgtatctgga tctcctggac gacgagctgc cttttgagca ggaatttctt tcccagtaat
      901 acgacgagcc gcctctaaca tctgaaggtt agaaaaacct gtcgaagatc cgagattaaa
      961 cacgtttgat tcattccctt ggcgaaggta atcaactgct aatatatgag catctgccaa
     1021 atcaaatgga tgcacataat ctcgaacatt agttccatct ggagtattgt aatcatctcc
     1081 aaaaatcatt attttgtcac gtactccttg agcaacttga agaatgattg gtaacaaatg
     1141 tgtttctggt ttatgatctt ccccaattga gccatccggt ttatctccag caacgttgaa
     1201 gtaacgtaga gcgacaaact taataccgta ggcttgatca gcccatttca taattgtttc
     1261 catcataagc ttactttcgc cataaggatt aataggattt tgaggagctg tttctaaaat
     1321 aggaacttgt tctggaatac catatgttgc agctgttgat gaaaagacaa tatttttgat
     1381 atcacattca ttcattactt ctaaaagttt tatcattcca gcagtgttat tatcaaaata
     1441 ttttaaagga ttttccattg actcagcaac tagtgaaaat gccgcaaaat ggataacagc
     1501 atctacatca ggattttccc taaaaacttg acgcataaac gttttatcgg ataaatctcc
     1561 ttcataaaaa atcgcatctg aatgcaccgc ttgacgatga cctgttacca agttatcaac
     1621 aacaataact ttctcttttc cttgtgtgat taactgatca accatatgcg agccaatgta
     1681 accggcgcca cctaaaatca gaactgccat atttttctcc tatttcaata ctcttttaac
     1741 atcatttgtt acctcattat actgagaata agcttttttt gcaaatatct accgaaatta
     1801 gaatagctct atgcagaagg catcccacgg cttgagagtg ttacttgata gtacttcttg
     1861 aacctctgta tttgaaataa tagtttcttt aacagcttct ggaaattcaa aagattggtt
     1921 ttgatcggat aagtttgcaa ctattaaata acgttcttta tctgtttgac gaacataagc
     1981 aaatacctta tctgctgttt ccaaaagttt aaaatcagca tcaactaacc agtcgtgttc
     2041 tttacgtaaa gcaactaatt tttgataagt ataaaaaata ctttccgtat catttaacgc
     2101 tgcctccaca ttaatttcct gataattggg attgactgct agccatgggg taccgttagt
     2161 aaaaccagct tgatgtcctt gactccactg cattggagta cgagcattat cacgaccgac
     2221 tttacgaata ctatctaaaa ttgttgcttc agatacccca ttatccatgg cttctttagc
     2281 atagttaagg gattcaatat catctacatc tgctaaacac tcaaatggat aattggtcat
     2341 accaatttct tcaccttgat agatgtaagg cgttccacgc atcaaatgta gtaggattgc
     2401 tagagccttg gcagattgtt ttctgttatc actatcattt ccccaaatcg agagcacccg
     2461 aggaaggtcg tgattattcc agaataaaga attccaacct tggccaagct ccaattgtgt
     2521 ttgccatttt gtaaaaatat ctttcaatgc tggaacatct aatccgtcac tgtaatccca
     2581 cttaggagcg tcaggtttat gttgaagacc tacatgttca aattgaaaga ccatagataa
     2641 ttcttcatta tcgggatttg agtattgttt tgcaatgtct ggcgttgctc cccaagtttc
     2701 acccaccgtc aataagtcat gctttccaaa actagctctg ttcatttctt taagataatc
     2761 atggagtttt ggtccattac cagtaatgcc cttatctgga attttcccaa ttaaatcaat
     2821 gacatccata cggaaaccac caataccctt atcaatccag aaattcatca tatcataaat
     2881 tttatgacgt aaagcttcat tctcccagtt gagatctggc tgacgtttgc taaaaaggtg
     2941 caaataatat tgacctgatg ctttatcata ttcccaagct gagccactaa aaatagaagt
     3001 caaatcgtta ggttcatctc gccaaatata aaaatcacgt tcaggactat tagggttttc
     3061 tcgcgcttct acgaaccaag catgttcatc agaggtatga ttcactacca aatccatgat
     3121 gattttaatc ccgcgttgat ttgcctcttg taacagttgg tccatatcat tcatgtcacc
     3181 gaaaatatct gcaatggctt gataatctga aatatcataa ccattgtcat ccattggact
     3241 ttggtaaaca ggtgataacc agatagcagt aatccctaat ttttcaaggt agtctagttt
     3301 gctagtaatc cctttaatgt cccccacacc atcaccatct gaatccataa atgaacgtgg
     3361 gtaaatttga taaatcgtag ctttatgcca ccagtgtttc ttcattattc ttctcctatc
     3421 aataattcat tcatctttta ttttaacgtt tttcctcttt tagtgcaagc gtttgcgcaa
     3481 ataacattat aaagaggctg gaacccaacc tttttatgaa tatcgcttat accttaacga
     3541 atagcttgtt cagtatctgc atcaaagaaa tgccccttag caacattaaa cgttaggtta
     3601 actttttctc ctggattatg gaagtcacgc gcttcaacac gagaagcaaa ttctgtttga
     3661 cctaacttaa cataaagcat agtttcgcta ccgagtaatt cagaaactaa aacttctgct
     3721 tcaacattgg catttggata agtatcttgg acaagaagat tgcttgaaat gtcttctgga
     3781 cgaataccaa aaatcaattc ttttccttta tagcctcgtg attctaaaag tttttcttga
     3841 ccttcaggga tagcgattcg aagaccatct tcactaataa tcataccatt ttcaacttta
     3901 actttgaaga agttcataga tggactaccg atgaatcccg caacaaattt attagcagga
     3961 agattataga gttcttgtgg tgaaccaact tgctcaattt taccaattgt accatcacca
     4021 tcagggttct tagtagctga cataatgaca atacggtctg ccaaagtcat cgcctctgtt
     4081 tggtcgtggg taacataaat agttgttgaa ccaatacgtt gatgaatttt tgcaatttca
     4141 gcacgcattg aaacacgtaa tttagcatct aagttagata aaggctcatc cattaagaac
     4201 actttggcat cacgcacaat agcacgtccc atagcaacac gttggcgttg tccacctgac
     4261 aaatccgcag gtttgcgctc taagaattca gtaaggccaa tattagctgc tgcttcacga
     4321 acgcgcttat caatttcttg ttttgagaat ttacgcaatt tgagcccaaa agccatatta
     4381 tcgtaaacag tcatatgtgg ataaagagca tagttttgga aaaccatagc aatatcacga
     4441 tctttaggtg atttgtcatt gacaacttcg ccatcaatct tgagctcacc ttcactaata
     4501 tcttcaagac ctgcgatcat acgaagtgtt gttgatttcc cacaacctga tggtccaaca
     4561 aaaacaataa attctttgtc tttaatatcc aagtcaaagt cttcaacaga ataatgcgat
     4621 gcacttggat attttttgta aatatgattt aaatttaatt ctaccattag attgacctct
     4681 tttattattg tgattttatt atagcaaaga aagggttttc atataatggt aaccttgcct
     4741 aaatttcttg tgcaaactaa ctaaatttta ggtatccatc gaaattaaat aggcataggc
     4801 taaatcatct aatattttta aattaagccc cgaagattgg gtaaatttat ctaatttata
     4861 ttgcaatgaa ttccgatgaa tgaaaagtgc cttagccgtt ttagcaaggt tcccttgctc
     4921 ttgccacaag gcttttatta tagccgaagt atcactcgta tcaaggatat gttgtaaaca
     4981 ttttgcttta atagaaggca tgggcgattg atttgccaaa gcccataaag ccatctttgg
     5041 aaatcgacag taatgttgtt cggccttgtg actaagaaaa agcgtaaaaa gtttatattc
     5101 ttcttcatat aattctttcc agtccactgc ttgtagatgc gtccaactat ttccaaaata
     5161 gcatcttaaa cgcgtgttaa agtcattttc aatcgttggt aaaatatctg ttagtaattc
     5221 cgtagcatta aagaaattat cctgatttaa aatcatgaca ctttgggttt gattaattgt
     5281 cataattgtt tctacgttta ttactaaagg cgacaagaaa tccttaagtt cttctgataa
     5341 ggtaaattga tgctcaataa aaataaattg gactgccgaa tgagattggg gcaaccttcc
     5401 acgaccttca actagatagc ggtaccaagg attctgagaa tccactgaac tcgcctcttc
     5461 caggctttgt gtcatttgta ataagcatag ttcacgctta gttagcgaag attttggaaa
     5521 gtggaggaca tgaccatctt gctcaacgat gatataatcc tcactctgtt taacttcctg
     5581 tttagtaata acttctggaa acagttcctt aaaatccatg gcatcctcca ctacagtttt
     5641 gttcattata tcatatttat aacctctttt tgagcacaga aaaagaggag aatgtctcct
     5701 cagaaagttt ctgttataaa atagtgatcc gaatagaatg ctgtttcgac tgctttggat
     5761 taagccgtac gatattttgt ttatcttcta aaatatcaga ctcttcaata gaagttgaca
     5821 aggaactcca gggttccaaa gccaaaaaag gaccaccatt gttacttgtc caaagaataa
     5881 gattctcaaa atcatcaaag tcaagttgga ttccttttgc atgatttcta gattttaagt
     5941 agaccgtctt agatcgtagt tggtctaaag taatagcatc tttttcaaat aaagcataat
     6001 taagagataa ttgtttttga ttttctaaaa aaggatgtct agcttgtaaa tctaacaagc
     6061 ctgtatccgg aaacgactca ggaattgtgc aagtttcaaa ctgttcaaac tctagatagt
     6121 aatcactaaa tacttccttt tcaaacaaag ggcaattaaa gcctgggtga gctccgatag
     6181 catatggcat atttttttct gattctaagt ttttgacaat atatgtaatg gcaatgcttt
     6241 tatttcttaa ggtgtaagtt atttctaaac taaaacgata aggataattt atcaacgttt
     6301 cttcactact tttaattgta aaacgaacag aagaatccga tatgtaatca tagtcgaatg
     6361 ttttgtgacg tacaaagcca tgccttggta tgatacccgt aaaatgtgga gcttgagctg
     6421 gtctgtaaat gacgcaatca tttcgaacac ttccgcatat aggaaataaa ataggagctt
     6481 gcccacccca ataagtgggt tccccttgcc aaaggtactc aacgccttga ctatctgtaa
     6541 tagaagtcat tgttgcacct aacgctttta atgtcacttg caactcatga ttttgtatcg
     6601 taatagccat tatttttcct ttcataaaga gcaataattg ctttaccaac ttttctctag
     6661 caattatctt tccttccaag aagttgctgt tttttgcaaa attttattta gttcatcaat
     6721 attttgaaaa cctattgtac gtaaccattg acgtgcacca gcttctcctt tttcaacata
     6781 ctctttaaca cttccagccc aagttgctct accacaaagg acaccgttaa atttagcacc
     6841 agcttcttta gcaaacacaa gagtctcttg aaaaagctga gcaggaactc ctgcactcaa
     6901 aaaaatgtag gggagtagtg tcgcctgatc ttgttctcta aaataagccg cagctgtcgc
     6961 tttattataa gcagtctctc cttgcgcaaa accttctaca tagtccatat tgacaggcac
     7021 ttccaccttc aagacatcaa tattaaatcg aggatttgaa aaaactttca tagcttcaat
     7081 aaccttacgt ggcttaattt tggcatactc aatacctgag ctatccgtaa tcttttcatc
     7141 atatgacaaa atttcaagga aaaaaggaat gtcttctgca acacattcag aaccaatccg
     7201 ttcaatataa gcttcttttt cttcgtttac ttcatccgaa ctatcaacat cataataaag
     7261 taagaactta actgcgtctg ccccctcttc tttaatgcgt ttcgcagacc aaatgtctaa
     7321 acagtctggg agtcgcttgg tactactggt atcataacct gttttttcgt acgcaagtaa
     7381 aagaccggca ttgtcatcta aaactttcgt agctggcaac ccatattctg gatctaacaa
     7441 cattgatgaa gcgtaagggg ttaattcttc agctaccaaa actttaagtg cttctatttg
     7501 agaaacagtg ggctcctcag attgatattt tgccattaat cgcttcaatg ctccacgttg
     7561 gtcaaaagcc aaggccgaaa taataccatt tttatcactc aattgttcca tatgtttttg
     7621 tttttgttcc gttaatccca tcctacacct ctttcacttg gatttgactg attaattctt
     7681 gatagtaggt cagattgata tgccctgtta atgtttcttg tgcattaagc atacccaaga
     7741 cattagctct cttaagtaaa tttatatcat ctagttgatg ttctaaagca gacgcgattc
     7801 ctgcaaccgt tgcatcccct gaaccaactg gattgataac ttctattttt ggaatcgtca
     7861 cttgatagta gtgattccca tgtttagcaa aagctcccct agcacctaag gaaacaataa
     7921 cccaatcaat tcctctaaaa atatcttgct gtaaggtaga ttttaattcg tctaaactat
     7981 agctaattgg ctttccaatt aattgtgtta attcttcaag gttaggttta atgacggtcg
     8041 gcttagcact agattttaaa accgaacgta atgcttctcc tgaacagtct aaaacaactt
     8101 tcttattaaa gtgattcgct agggagatta acctagcata ataatctgag gggagtccct
     8161 ttggtaaact tcctgatact gttatgatag ttgcagcatc aaagataagt tttagatgat
     8221 tcaaaaaggc gcctgcttca tctttagtaa tatgtggacc tttttctaga atttcagtct
     8281 gctgaccctc atgtaaaact gcaatacaat gacgtgtttc tccaccaata cttaaaaatt
     8341 ggtgtctgat ttccaatgca tctaagccac ttcggatgaa atctccaaaa tgccccccta
     8401 atagaccagt tgccactaca ttatcgccaa gttgagaaag aacacgagtc acatttaatc
     8461 ccttaccacc aggtgttttg gagacgtcgg taacccgatt
//
LOCUS       DATROY010000078         8497 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.079, whole genome shotgun sequence.
ACCESSION   DATROY010000078 DATROY010000000
VERSION     DATROY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(43..1305)
                     /gene="dltD"
                     /locus_tag="VBD11_001398"
     CDS             complement(43..1305)
                     /gene="dltD"
                     /locus_tag="VBD11_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6954345.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
     gene            complement(1298..1537)
                     /gene="dltC"
                     /locus_tag="VBD11_001399"
     CDS             complement(1298..1537)
                     /gene="dltC"
                     /locus_tag="VBD11_001399"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6954346.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            complement(1552..2817)
                     /gene="dltB"
                     /locus_tag="VBD11_001400"
     CDS             complement(1552..2817)
                     /gene="dltB"
                     /locus_tag="VBD11_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6954347.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            complement(2814..4349)
                     /gene="dltA"
                     /locus_tag="VBD11_001401"
     CDS             complement(2814..4349)
                     /gene="dltA"
                     /locus_tag="VBD11_001401"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6954348.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCNRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGSAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            complement(4362..4484)
                     /locus_tag="VBD11_001402"
     CDS             complement(4362..4484)
                     /locus_tag="VBD11_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6954349.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            complement(4495..5682)
                     /locus_tag="VBD11_001403"
     CDS             complement(4495..5682)
                     /locus_tag="VBD11_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6954350.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            complement(5682..6356)
                     /locus_tag="VBD11_001404"
     CDS             complement(5682..6356)
                     /locus_tag="VBD11_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6954351.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            complement(6712..6846)
                     /gene="rpmH"
                     /locus_tag="VBD11_001405"
     CDS             complement(6712..6846)
                     /gene="rpmH"
                     /locus_tag="VBD11_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6954352.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            complement(7030..8385)
                     /locus_tag="VBD11_001406"
     CDS             complement(7030..8385)
                     /locus_tag="VBD11_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6954353.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLF
                     F"
BASE COUNT         2873 a         1610 c         1323 g         2691 t
ORIGIN      
        1 tgattaatag ctctaatgct ataaatcatc tgactctact atttacttgt aatcacttgg
       61 acgttctgta tattttgccc aagatttctt caaaaattta ttattaatgt gataattcgg
      121 cttgctgttt tcctctgtta aaaatggatt gatatgctta tctagctcta accaaccatt
      181 ccaacctaaa tggattgtat cttgcataaa gtatggctta cctccatctc gagaaaggtc
      241 agcgatatga ttgaaacctt gactttgaag ttggtgtttt attttttcta cagatttttg
      301 atacattttt tgatcaagcc ctgtgtagtc tgtccacttt ttatttactg gtggtatgac
      361 aaaaaggaca tctgtatttt gttttgagaa ttcattaaga accaactgta aatcattaaa
      421 ttctggtgac ttagtataat tgaaatgtcg ttgtgaccct ttcaattttt ttaagtgctt
      481 tttaattctt gtttggtaaa agcgatcatc aataccaaat tgatttgatc tcgttgctac
      541 tttagcatct tgtgatgcaa tacgcgataa atgattatat gagaatgctt tcggtaattt
      601 accaacatga ggcattattg cctgattata agtttttcct agtggtagat agctaaaaat
      661 agcatctgtc ttctcaaata cccaatcatt aaactttagt ctttgtttgt ctttatttga
      721 tagtttttgg ccatctgcaa ctttttctat caaatctgac atagaagctt caggatataa
      781 ttttaacaat cgacgagcag catacctatc gtaggttgtc cctgtttgat tttgcaaaaa
      841 tcgaatggtt tgatcgttgc tgaaatagtt ttgaaaagca cctttgttgg ctcccttgcg
      901 aacaaaccat tgcggagaaa taacatagat agctttttta tttttgattt gtcctttaat
      961 ctgttgaatg ccatagtatt gtgtcagaga agccgcccct ttttgaccta aaagatatgg
     1021 gatataagac ctattgtaag cctctgctaa aactgatgga tgcattgcgt caaatcttag
     1081 ccactcactt gaaccaaaga aagggacaaa attggcttta ggatcactga aagctctaac
     1141 tttttgaact ctgcttttaa aacctgcctt cgtcaacgct actgcactgt ttttttcctt
     1201 acctaaatga tgaggctgag aaacaggaaa aacaaaatat aatcctacca acaatagtaa
     1261 agcacaaact agaggtccaa atactttacc tagacgttta agcattgcag agctccgtta
     1321 ctccagcaac aattttatta gctgtgttcc agtcattacg accaaattcc gcaatcggaa
     1381 tatcaatatt aaagtggctt tccaactcaa caattagttc aacagtaccc atgctgtcta
     1441 gaactcctgc gtcaaataag tcttcatcca tcatagaaga aacatcctcc ataaataggt
     1501 catcaataat tgctaatact tctgatttaa tatccattat aatactcctt attatttgtt
     1561 atgcggttgt gggaaccaca aaagatctaa aaatcctgaa aaaatcaaga aagaaaacat
     1621 aactacatta aaggtgatga agacacccaa agcataagtc catttgtttt ctggtaaaag
     1681 tggtttttta gccaatcttc tttctttatt aatatttttc ttcttacgta cccaagcgtc
     1741 attaataact aggccaatcc catgaaagag accataggct atatagtacc aagttaaccc
     1801 atgccagaat cccataagaa gcatattgat aatataagct acacttgaag taacattacg
     1861 gtttttaaaa actttatttt taactaaaag cttaacaagc ctcatgaaaa caaagtctct
     1921 aaaccagaaa gaaaggctca tatgccatct attccaaaat tcttttaggt cgcgtgattt
     1981 gaaaggtttg tcaaagttaa tcggagactt aatccccatt aggttagaaa tagccaacgc
     2041 aaacattgtg taacctgcaa aatcaaagaa caaatcaaaa ccaaaggcat acataacccc
     2101 aagtgttggc caattaaaca caccaccctg ttctagcgct aattctttta gaggaggcat
     2161 aatcagactt ccaaaaatat aggctaaaat aaacttatag agaaaaccta acatcaaata
     2221 atgaatcgct tgacctaaca tatctaggag ttcgtttcga tctgggatat taatgtaatc
     2281 ctcattgaat cgtttaaaac gatcaattgg tccacttgag aaagttggaa agaagagtaa
     2341 aaatcttaag aattcccata aagtaaattc tttcaagaca ccgtctctca tttcaataat
     2401 catagcaaca gctctaaaag taaggtaaga aattcctaga aacccaaaaa gtgactgatg
     2461 cccatcctca gatatagctg gaattaattt taccaaaaat agcggaaaaa tagataaaca
     2521 aaccgttaca taaaagacca aagtatgatt aaatcgtttc cggtaagctt tatagataaa
     2581 aactagcaga gactgtccga caacgtatgc caatagtgac ttcaattgat taaaagtact
     2641 accagtcaac atcattacaa taaaacttag actaaaaata atctcatata aggcaaaacg
     2701 cttcttaaag aataagccga tgtaaattgg taggacagct aagataaggt agaagaaata
     2761 ttgtggattc ccataaacat ccaagtgagg aagtttttct aaaaatgcct gtatcacttg
     2821 tttacctcgc tcataagacc tttgatatca attttcccat ttggtgtcaa aggtaaatcc
     2881 tctcgataga taaatttaga tggcatcatg taatccatca taatgtcctt taagtcttcc
     2941 ttaattgctt ttgtcaaatc caaatcacgt tcaaaatcat ctcttacacc ttcttttaag
     3001 acaatatagg ctaataagtt ttgaacttta tgatccttgt tataacgtgg cactgctact
     3061 gctgatttta catactgcga tttgtttaag ttttgagaaa catcttctaa ttcaatacga
     3121 tagccgttaa atttaatttg gaaatccata cgcccaccgt aaagcagaag accttcatcg
     3181 gtcatacttc ctaagtcacc ggtatggtaa gcaggtagac cttcgaattg gaaaaatgcc
     3241 tcagctgtct tttctggatt gttaagatag ccttttgata ctgctgatcc agcaataatg
     3301 atttcacctt gctctccgtt tggtaattta tgaccttctt catcaatcac atacgttggt
     3361 gagtcatctt tagtgtaacc aattggaagg cggttgcatg tttctaacat ttcatcagta
     3421 attgctactg cggatagagc aactgttgct tctgttggcc catatgcatt aacgatacga
     3481 gcttttggaa aacgttgacg aagtttttgt gcagtcttga cagttaactc ttccccatca
     3541 aaataaaaat gtgttaactg tggcaaggtc tctgaattga aatcgttaga tagtagcgcc
     3601 atatcagcaa atgatggtgt cgaagtccaa acctgtattg gcaattcatt aatggtagcg
     3661 aatagttttt tgaaatcatt aactactgtt tttggtaggg caaacagggt gcctcccata
     3721 gctagtgttg gtgcccaata cataactgat aagtcaaatg aatatggcgg ttgagccaac
     3781 atttgcggtc tttcaggaac tgaaaactca tcatcagaaa tcatccaatt tgtaaagctc
     3841 aataaattgt catgtgaaat ttgcacacct tttggtaaac cagtagtccc tgaagtgaaa
     3901 ataatatagt aattatcatc acctttaaca gaatgtgtta cctcataagg agtcttttct
     3961 tcaaaaatag ctgaaacttg agaaacgtct aggattggga cattatcaac ttcaagagga
     4021 aattcaccaa ttgaaatgat aaggcttggt tgagcaactg tcataatagc ctgtattcta
     4081 tccaaagcag agtgttggtc aaccggtata taagcatgcc ctgactttgt taaagcaaca
     4141 aatgtcgcca acatttcata ttcttgacca ccgaatacta agacaggtga tttttcaaca
     4201 aggcctaggc tatcaatatg agcagctaga gagtcagagt ctactttaag ttgtccataa
     4261 gtatggactt cccctaaaat atcatacact ggaaaatcag cttgtgtctc agcaaaatgc
     4321 tcaattgttt taatcatatc atgtatcatt tgtaccatct cctagaattc attataaata
     4381 aaactgcctt gtccgcgacc aaggtatgca aaaaagtaaa ttagtaacaa taaaataccg
     4441 aagtataata acgttctacc aataaaagat aaatatggtt tcatcattct tcctttaatt
     4501 aatcatggta ttggtccaaa atccatcaat tggtaactgg acttcaagac gtaattggtc
     4561 tttttcttga tatgctctaa ttctcccctt atggcgttcc acgattgctt tagcaataga
     4621 taagcctaga ccaaaactga gagaatctgc atgatcatct ttagcttgat aaaaacgctc
     4681 aaagagatta tccataacct ctttggaata gatagcactt ggatttgata ttgtcagaat
     4741 agcttttctt cgagcttgtt taagactaaa ctggattgct gattttgaca aagaatgcct
     4801 aatagcatta tccaaaagaa ttattaaaat ttgcttaatc tgactctctt ctccaacaat
     4861 ccagacatca tcttcaatcg tatcatttaa acacttttct tcttcatatg ccaaactttc
     4921 atatgttaga agtacttctt gacataagac agataagttt aactttttga aatttacctt
     4981 ggagcgtttt tcatcaagtc tagacaaatt aagcaaatct tcaattaaaa actccattcg
     5041 cttagtctcc gaaacaacat gatccagata acgattccct ggaatttgct gttccagaac
     5101 ctgaacgttg gctcgtatag ctgcaatagg ggtcttcagc tcatgactcg catctgaaac
     5161 gaactgtttt tcgcgagtca tttctgtctc tacaggtcct acaataaatc gagaaagata
     5221 aagacttaca gctgctaata aacaaaatcc aaaaatagta aaaacaagaa ttagaaaacg
     5281 ccttgcataa gggacttcat aactagccaa atccataata gctatggtgt aggttttatc
     5341 tttatattct tttatggaat atataaaaga cttccatttt cgtgacttct ttccttgctc
     5401 aaggagcttc aaactagatt tttcgataac atcatttgtc aaatcagtat tattgctttg
     5461 aatcttaacc atcttttggt tatctatagt caccaaagca atcggttcat cgcctggtat
     5521 tcctaaataa tcatttttgg caactaattt aacaatccta tatgcatcat attcatccca
     5581 atattgattg tagcgattac taactgcaaa tagaaagaga acaacgacaa taaggattga
     5641 catggtcaaa aagacaaatt ttttgcgtaa atcactaaac attatgtcct ctcctccaag
     5701 gaatatccaa agcccttagt ggttattatt tcaatgtctg cttttaaaaa gcgcaatttt
     5761 tttcgcaaaa aagaaataaa tacttctaac tggttatatt ctgaattgtc tgaaggtccc
     5821 caaaccttag aaatcaactg atttttagga ataatctgct ttgcatttcg cataaaaact
     5881 tccatcaaga gaaattcttt attggataat tttacactac tttctttaga ttctagctca
     5941 tgtgaatctg tgttaagcct tatatttcca agccgcaatt gatttgctct gattagagaa
     6001 gattgtctgg ttctcagcct aattctagct agcaattcat ctgcttcaaa gggttttgtg
     6061 atataatcat ctgcccctaa atctaaacca ttaactttat cataagtctg tgattttgca
     6121 gttaaaaaga ttattggggt ttctaaacca gcttccctca ctctttttaa aacagataat
     6181 cctgagcgtt tgggtaacat aatatccaac aggactaaat catagagacc tgtcaaaatg
     6241 tattctaacc catcatctcc atcaaatgct aaatcaacag aataaccttt atctgccaat
     6301 aaagcctgaa tagcctctgc tatacttttt tcatcctcaa ctacaagaag tctcattttc
     6361 tagtcacctt accttatata ttatagtata aactagcaaa attgaaataa ccttgaaaaa
     6421 acgatattaa taaaagtatt tcctaagtca ctaaactact tcaataatac aagcgtctaa
     6481 aaaataaaat atcactcgct ataaagatat ttctcaataa gaaatctcct catattctaa
     6541 caaaaatagg ggctatttgt ctacgaattt ccgcattata aactcacttt tcattaactt
     6601 tatctataat atactcaaac tactctcaca aaaaccttta tcgttatgaa ggtaccaaaa
     6661 aagccaccag actcgagtac ctgttggctt gatattattt tgctatctat attaagctga
     6721 taacacttta cgtcctttac gacggcggct agcaagcacg cgacgtccgt ttttagttga
     6781 catacggtga cggaatccgt gtttacgttg gcgacggatt ttacttggtt gataagtacg
     6841 tttcacgttg aatacctcct gatagatttt cgtattcgtt tagccggcta ttttgatcag
     6901 ttactaaaca taccatacta ttttataaca attttaggcc tcttgtcaag aaatactttc
     6961 aagttcattt gtcttttttc agtaaatagt tctatttgtt agcaagaaat gcttctctat
     7021 tttctccaac taaaagaaaa gatgtgccat caaaacaata ataggtaatg aaataattgt
     7081 tctctctata aaaatgataa ataattctag aatgttgata gggatcttgg aacctaatat
     7141 caccgcacca gtctcagaca tataaattaa ttgcacaata gaaactgttg cgacaataaa
     7201 ccttgtcatc tcactgtgaa tttcagccgc cattatcgaa ggaacaacca tatctgaaaa
     7261 accaacaatc atggtttgac tcgcaatatc tgcttctgga atccctaaaa gttcaaggaa
     7321 cggaaggaat ggtgtaccta aaattttaaa aataggtgtt gatactgaaa taatcaaagc
     7381 tattgttccg acagtcataa ttgttggcat aacgccaaac cataaactaa gtactgtctt
     7441 agaggcatta ttgacaaaag tcttcatgtt tccatttgct tttgatttat cgatggcaag
     7501 cttaagtccc cactctatct tgttataacc atccggaatt acctcagatg tttctttctt
     7561 tggtttagtg taataactat ttgattgacg agataaggga gggatataag ggataatgaa
     7621 tgcacagaca acactgctaa tgatgactgt tagataaaaa tggccaaaat aatcagttaa
     7681 gccaacattc tgcaaaacaa caagacaaaa agtgattgat actgctgaaa aagtagtgga
     7741 aatcgttgca gcttctctac tggtataata accctcaaca tactgcttat cggtcaaagc
     7801 aattccaata gtaccatcac ctacaaatga ggctacacaa ttaactgctg accgaccagg
     7861 tagctgaaag aatggacgca taatgggagt taggaagaca ccaacaaact ccagtagacc
     7921 aaattctgtc aaaaatggaa gaataaaacc cgcagcaagg aaaatggtaa acaaaccacc
     7981 aatcaaatca aaaaggataa ggccgcctgt ctctttccca cataccattt caggaccaat
     8041 atgtaaaacc gtcatcagac caagtgccaa accgatcaaa cgaataatca gccagaaaat
     8101 ggtaatgtca gaaatttctt ttagcaagtc atttttttca ataaagctag gtctaaacag
     8161 acgataacaa agtgctaaca cacatgaaat aaaaatgatt agtaaaataa tataatgtat
     8221 aggaaaaaca gcgttaatcc attgattaat tgtcttagag attacagaga cagctactgt
     8281 tgtcatacca ttatatttaa atggagtcat taataataat actccaaata acgacggaat
     8341 aataaaccac aagatatctt taatagatat actttctttt ttcatacaat cctcctggat
     8401 aatatggaat tgtttctaaa tatactcata acttccattg taaaatttta taataaaaaa
     8461 aacaaccttg aattaaagaa aattttactt agtaata
//
LOCUS       DATROY010000079         8337 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.080, whole genome shotgun sequence.
ACCESSION   DATROY010000079 DATROY010000000
VERSION     DATROY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..246)
                     /locus_tag="VBD11_001407"
     CDS             complement(<1..246)
                     /locus_tag="VBD11_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954354.1"
                     /translation="MKVSTEKTIVKKNWKSYIMMIEATKQLKEISALAEARQSSSQEF
                     LVTQTEILVFVDKNGEFDEEILKLINDNYEIIAKYAKI"
     gene            502..1920
                     /locus_tag="VBD11_001408"
     CDS             502..1920
                     /locus_tag="VBD11_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6954355.1"
                     /translation="MNDKKQRFSCTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLK
                     VMTSKDQPRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATE
                     TITTNSRVLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIA
                     FSDTEEIWWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFI
                     EQYHLDLTYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWC
                     QKPYRKITVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRP
                     NKSLETTGVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAAL
                     ADPHFYQHEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQ
                     ELLNRILFDASNLMTNRFSMGD"
     gene            2062..3582
                     /locus_tag="VBD11_001409"
     CDS             2062..3582
                     /locus_tag="VBD11_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6954356.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(3695..3955)
                     /locus_tag="VBD11_001410"
     CDS             complement(3695..3955)
                     /locus_tag="VBD11_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6954357.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(4064..4999)
                     /locus_tag="VBD11_001411"
     CDS             complement(4064..4999)
                     /locus_tag="VBD11_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6954358.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            5265..6287
                     /locus_tag="VBD11_001412"
     CDS             5265..6287
                     /locus_tag="VBD11_001412"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6954359.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            6346..6789
                     /locus_tag="VBD11_001413"
     CDS             6346..6789
                     /locus_tag="VBD11_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6954360.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            6866..7141
                     /locus_tag="VBD11_001414"
     CDS             6866..7141
                     /locus_tag="VBD11_001414"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6954361.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            7134..8330
                     /locus_tag="VBD11_001415"
     CDS             7134..8330
                     /locus_tag="VBD11_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6954362.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
BASE COUNT         2667 a         1301 c         1628 g         2741 t
ORIGIN      
        1 aatcttagcg tatttagcta taatctcgta gttatcattt ataagtttta atatttcttc
       61 atcgaattca ccatttttat cgacaaagac aagtatctcc gtttgtgtga ctaaaaattc
      121 ttgtgaagaa gattgtctag cttctgctag tgcagatatc tcctttaact gcttggttgc
      181 ttctatcatc attatatagc ttttccaatt cttttttact attgtttttt ctgtgcttac
      241 tttcatttta gtaacctctt tgcttatctt taaatatatt atagcatagt gcatacacta
      301 tgtaaagtgt tttgttttat ttttttaaaa tatttatctt tttgtctatc acagtggaaa
      361 attttaaaat tgtctatttt taggattttt ttacgaataa ataaagaaga agtgttgaag
      421 ggtttagagt gttcgtctat tttgtgtaaa gtgaaatgat atctgatata atgatagaaa
      481 aaagttaaag gagtagttaa gatgaatgat aaaaaacagc gtttttcatg tacaacaata
      541 ttggttggta aaaaggcttc ttatgatggt tcgactatga tcgctagaac ggaagactct
      601 gttaatggcg atttcacacc caaaaaatta aaggtaatga catctaaaga tcaaccgcgt
      661 cattacaaat cagttttatc aaattttgaa gtagatttac cagataaccc acttccttat
      721 acttcagtac cggacgcatt gggaaaagat ggtatatggg gtgaagccgg tattaacagt
      781 aaaaatgtag cgatgagtgc tacagaaact attacaacga attcccgcgt tttgggtgca
      841 gatcctttgg tttcagatgg tataggggaa gaggatatac tcactttagt gcttccctat
      901 attcagtcag cgcgagaagg tgtggagcgt ttaggtgcta ttttggaaaa atatggaacc
      961 tatgaatcaa atggtattgc tttttcagat accgaagaaa tatggtggtt agaaacaatt
     1021 ggtgggcatc attggattgc tcgtcgcgta cctgatgatg tttatgttac taatcctaac
     1081 caactaggaa ttgatcattt tgaatttaat aactgtgatg actacatgtg ctctagtgat
     1141 ttgaaagagt ttatcgaaca ataccattta gatttgacct attctaatga gcatttcaat
     1201 cctcgatatg cttttggtag ccaacgtgat aaagatcgtc attacaacac accaagaagt
     1261 tgggcaatgc agcgtttttt aaatcctgaa attgaacagg atccacgtag tttgtttatt
     1321 ccctggtgtc aaaagcctta ccgaaaaatt actgttgagg atattaaata tgtgttgagt
     1381 gatcattatc aagacagtgt gtatgaccca tatggaccag aaggggatgc ggtaagtagg
     1441 agagcttttc gttcagttgg tatcaaccga actagtcaaa cgtctattct acaattgcga
     1501 ccaaataaat cacttgaaac gacaggtgtt caatggttat cttatggctc tatgccattt
     1561 gcaaccatgg tgccgttgtt tacacaagtt gagactgtac cagactattt ttcgaataca
     1621 accaaggatg cttcaacaga taatttttat tggaccaatc gtttaattgc agctctagca
     1681 gatccacact tttatcaaca tgaagctgat attgaaagct atatcgagag aacgatggcc
     1741 caaggacatg cacatattaa tggtattgat agagaagttg ctgagaataa agagattgat
     1801 tttcaacaga aaaatcaaga aatgagtgac tatattcaaa aagaaagcca agaattgtta
     1861 aatcgtattc tatttgatgc aagtaattta atgacaaatc gcttttcaat gggagattaa
     1921 aaaactcgga atattttccg ggttttttaa gataaaaacg cttgcataag tatataaaac
     1981 ttgcaaaaaa aaaatatcgt gctatacttt actagtaaaa tattaaccgg taaataacgg
     2041 gttaaataaa aaggagaaaa aatgagaaag aaatttcttt tattgataag ctttgtagct
     2101 atgtttgcag cttggcaact tgttcaagtt aaacaagttt gggctgatag taaacttaaa
     2161 gtggtaacaa ctttttaccc agtttatgag tttacaaaaa atgtcgttgg tgataaagct
     2221 gatgtatcta tgttaattaa agcaggtaca gagccgcatg attttgaacc atcaactaaa
     2281 aacatcgctg ccatccaaga ttcaaatgct tttgtttaca tggatgataa catggaaact
     2341 tgggctccaa aagtagctaa gtcagttaaa tccaaaaaag taacaactat taaaggtact
     2401 ggcgatatgt tacttactaa aggcgtcgaa gaagaaggtg aagaacatga aggacatggt
     2461 catgaagggc atcatcatga acttgaccca cacgtatggt tgtctccaga acgtgcgatt
     2521 tctgttgtag aaaacatccg taataaattt gtcaaagctt atccaaaaga tgcagcttca
     2581 tttaataaaa atgcagatgc ttacattgca aaattaaaag agcttgacaa agaatacaaa
     2641 aatggtttgt caaatgctaa acaaaagagt tttgtgactc aacacgcagc gtttggttac
     2701 atggcgcttg attacggttt aaatcaagtt ccaattgctg gtcttactcc agatgcagaa
     2761 ccttcatcaa aacgtttagg cgaattagct aaatacatca agaaatataa catcaactac
     2821 atttattttg aagaaaatgc ttcaaataaa gttgctaaaa ctttagcaga tgaagttggc
     2881 gtgaaaacag ctgtgcttag tccacttgaa ggactttcta aaaaagaaat ggcagctggc
     2941 gaagattact tctcagttat gagacgtaat ttgaaagttc ttaaaaagac aacagatgtt
     3001 gcaggtaaag aagtagctcc tgaagaagat aaaactaaaa cagttgaaac aggttacttt
     3061 aaaactaaag atgttaaaga ccgtaaattg acagattact ctggtaattg gcaatcagta
     3121 tatcctcttc ttcaagatgg aacacttgat ccagtttggg attacaaagc taaatctaaa
     3181 aaagatatga ctgctgcaga gtacaaaaaa tattatacag caggttacaa gactgacgta
     3241 gaatcaatca agattgatgg taaaaaacac caaatgacct ttgtacgtaa tggtaaatca
     3301 caaacattta catacaaata tgcaggttac aaaatcttaa cttataaaaa aggtaatcgt
     3361 ggagtacgtt atctctttga agctaaggaa aaagatgctg gtcaattcaa atatatccaa
     3421 tttagtgacc atggtattaa accgaataaa gctgaacact tccatatctt ctggggttca
     3481 gaaagccaag aaaaattatt tgaggaaatg gaaaactggc caacatactt cccagctaaa
     3541 atgtctggac gtgaagttgc ccaagacctt atgtctcatt aatatttaaa agatgatatc
     3601 ggatacgata tcatcttttt ttcttcgtac gtgataggct caaacataga aaaaagcact
     3661 tctattagaa gtgcttttaa aattaatgct atgcttattg agcagcgttt gcgtctttga
     3721 gaccatattt tttgttgaaa cggtccacac gtccatctgc ttgagtgaat ttttgacgtc
     3781 ctgtatagaa tgggtgtgaa tctgatgaga tttccacacg gataagtggg taagtttcac
     3841 cttcgaattc tactgtttct ttagttgatt tagttgaacc actaaggaat ttgtaacctg
     3901 tagttgtatc taagaaaaca actggacgat aatctggatg gatgtctttt ttcatcttta
     3961 aaaatttcct ttctgccata actcgtctga gtcatagtta ttaaccactc tattctatca
     4021 aataagaata gccttgacaa gtgttttgat aaaaaaagta aaattataaa gaaagtactt
     4081 cctggatttc ttggtaaata gcttgattct cttccaaact ataagaatta gctccactgg
     4141 ctaaaggatg gccgcctcca ttatgacgtt tagctatttc attaatgatg tgtgacttac
     4201 tacgtaggcg cacacgataa tgcccatcgc tttgttcaac gaaaattgcc caactttcta
     4261 caatatcaat ttttccaggt ataccaacga ttgcagcagt ctctgcatct gttacatcaa
     4321 agcgttttaa gtcttcttgt gttaaagtaa cgcatgcagc gccgttcttg tcaatttcaa
     4381 gttgctcaaa aataaagcct tgtaattttg caattttgaa aggaaaggaa tccatttgac
     4441 gtgccattgc agaaaaatca aagtcaaatt cacgaagttt gctagcaatc ttcagtgttt
     4501 tacttgtggt agcaggatat aaaaagcgtc ctgtatctcc tacaatacca ttatatagaa
     4561 ctcttgctgc acttgttgat aacaacaaat cacaagaaag agcaaagtct gtcacaattt
     4621 cacttgcact agaggcgtta gtatctacat aagagaggtc tccatagact tcatcgtttg
     4681 gatgatggtc aattttaatt aaaaagtcac cttttttata acgttcatca tcaatgcgag
     4741 gggtatttgc tgtatctgta acaataacaa gagcgccttg atagtcttgg tcggtcactt
     4801 gatccatttt agcaatccaa gcgagtgtgg gttcgtcaaa accagtagca agtacttttt
     4861 tctttgggaa attatgcctg atgatatccc ttaagccgat ttggctacca agagcatcag
     4921 ggtctggtct catatgtcga tggataatga tggtgtcgta ctctttgatt ttatcaagaa
     4981 tttgttgaaa aataatcatt ttttacctac ctaactgttt tgtcttttag tgaagaacaa
     5041 ggctatattg ttgactatat tatagcttaa atcttttatt gaggaaagaa attaagatag
     5101 cggccgcaat gtttgtttat ttttatatga tggtatgtta tttttttata aaattcggat
     5161 actgctaact aacaatcttt tttatgataa tattttgtaa atgtattgct ttttagctaa
     5221 cttttgttaa tatagtaaaa atgcttttta ataggagata aattatgaca caagcagtat
     5281 taaaagaatt ggctaaggcg gagttgcatt gtcatctaga tggctcactt tctttaccag
     5341 ctattagaaa attggctaat atggctgata taatactgcc aaatagtgat aaagaattaa
     5401 gaaaatatgt tatagctcca gctcaaacag aaagcttggt agattacctt aaaacgtttg
     5461 agtttatcag accgcttctt caaacgaagg aagcattacg ttttgctgct tatgatgttg
     5521 ctagacaggc agctttagaa aatgttattt atattgaaat tcgttttgcg ccagaactct
     5581 ctatggataa aggtttaacc gcaagtgata cagtctttgc agtcttagaa ggtttagcag
     5641 atgctcagaa agaatttaat atcgttgcac gcgcccttgt ctgcggtatg cgccaatcgt
     5701 ctcataaaac gaccaaggaa attattaaac acattgttga tttggctccc aaaggcctgg
     5761 taggttttga ttttgcaggt gatgaatttt cctatccaac agatagttta gtagatctta
     5821 ttcaggaagt aaaacgctca ggttacccga tgactttaca tgcaggtgag tgtggttgtg
     5881 ctaagcatat tgcggatagt ttaaatttag ggattaagcg tatgggacat gtgacagctt
     5941 taacaggaca gagggcttta ataaagcgat ttgtcgaaga agatgctgta gcagagatgt
     6001 gtttaactag caatttacaa acgaaagcag cctcaagtat tcaaagtttt ccttatcaag
     6061 aactttatga tgctggaggt aagattacaa ttaatacaga taatcgtact gtttccgata
     6121 caaatttgac gaaagaatat agtcttttcg taacttattt tggaacgaaa atagaagact
     6181 ttcttgtttt taatcaaaac gctgtgaagg cctcctttac aagtgattca gagaaagata
     6241 ctttactaca taagctacaa gaaaactatg acagctatct aaaataacct tcacgattct
     6301 ttaaaaatat gatagaatag tggtaattta caagtggagg aagatatggc tttagcaaag
     6361 attgtttatg ctagcatgac cggaaatact gaagaaattg ctgacattgt tgctgataaa
     6421 ttacgtgact tgggtctaga tgttgaagta gaagagtgta cgatggttga tgctgcagat
     6481 tttgaagatg ctgacattgc aattgttgcc acttatacat atggtgatgg agatttacca
     6541 gatgaaatcg tggactttta tgaagattta gcagaagttg atttatctgg taaagtttac
     6601 ggcgtagtgg gttcaggtga tactttctat gattacttct gcaaatctgt tgatgagttc
     6661 gaggctcaat tcgcattaac aggagcacaa aaaggtgctg attgtgttaa agttgattta
     6721 gcggcagaag acgaagacat tgaaaactta gaagcttttg ctgaagaaat tgcttctaaa
     6781 cttaactaag ctaccaaaaa gacgaacacg agtcgtcttt ttaacttttt taccatcttt
     6841 tgtaactaga atatgagata tagatatgga attagaagag attcgtcaag aaatagatga
     6901 aatagatcag caattggtaa gccttttaga aactagaatg ggtctaattt tagaggtcat
     6961 agcatttaaa aagaaacacc gacttccagt tttagataat aatcgtgaaa acgaagtgct
     7021 taacaatgta ttgaagaaag tccaaaatca tcaatttgac gatgtgatac gagcaacttt
     7081 caaagacatt atgacagaat ctcgtgttta tcaaaaggag aatattgtag acggtgatta
     7141 aacgatttga taattatgct cagtacgtac ttgttttagg gtttagctta ctaatcggtg
     7201 gtgtggttgg ggctattaca gcagtctttg gacgtgttct attattcctg acagccttta
     7261 gaagtgatta tatagcttac ctactccctt ttcttagcat tgttggttta tttatagttt
     7321 ttgtctatca aaaatttggg ggtaagagtg ttaaagggat gggattggta tttgaagtag
     7381 gtcatggtaa tgaggagact attccgaaac gtcttgttcc tctcgtgata ttaacaactt
     7441 ggttaaccca cttatttggt ggtagtgcag gtcgtgaggg ggttgctgta cagattgggg
     7501 ctaccgtttc acactacttt caaaaatatt gccgtttaca aaacgcttct caacttttcc
     7561 tagtaatggg aatggcagca ggttttgctg gtttatttca gactccttta gctgctactt
     7621 tttttgcgat cgaagttttg gtagtaggtc gtttaatggt ttcgtatata ttaccaagtt
     7681 taattgctgc tctgacagca aatttcgtct cacattcttt aggtttagaa aaattttctc
     7741 atagtatagc aacatcaatg gcgttaacac cagatattat tctaaaatta ttggttttag
     7801 gactatgttt tggtctgtgt ggcaatttat ttgcttattt attggctaaa gctaaattaa
     7861 ttgcttctag taggttacta aacccatata aacggatttt cacacttgga ttgttagcta
     7921 cttttttatt gtttatcttc cattttggac gatattcagg actgggaaca aatctaattg
     7981 aggctagctt cactaataag aatctttatg attatgattg gttgttaaaa ttgtgtttga
     8041 cagttattac tttggctgca ggttatcagg gaggagaagt aactccctta tttgcaatag
     8101 gagcaagttt aggagtgatt attgcgccca ttttaggttt gccagttatt ttagttgctg
     8161 ctttaggcta tactagcgtt tttgggagtg caacgaatac tctgttaggt cctatcttaa
     8221 taggtggcga agtctttggc tttgccaaca ctccttattt tgttattgta tgtcttgttg
     8281 cttactccat atcacatgct catacgattt atggtgctca atctagatag ttagtga
//
LOCUS       DATROY010000080         8284 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.081, whole genome shotgun sequence.
ACCESSION   DATROY010000080 DATROY010000000
VERSION     DATROY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(97..450)
                     /locus_tag="VBD11_001416"
     CDS             complement(97..450)
                     /locus_tag="VBD11_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6954363.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            complement(447..1178)
                     /locus_tag="VBD11_001417"
     CDS             complement(447..1178)
                     /locus_tag="VBD11_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6954364.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            complement(1180..2121)
                     /locus_tag="VBD11_001418"
     CDS             complement(1180..2121)
                     /locus_tag="VBD11_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6954365.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            complement(2111..3265)
                     /locus_tag="VBD11_001419"
     CDS             complement(2111..3265)
                     /locus_tag="VBD11_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6954366.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            complement(3382..4236)
                     /gene="rfbD"
                     /locus_tag="VBD11_001420"
     CDS             complement(3382..4236)
                     /gene="rfbD"
                     /locus_tag="VBD11_001420"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6954367.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            complement(4326..4667)
                     /locus_tag="VBD11_001421"
     CDS             complement(4326..4667)
                     /locus_tag="VBD11_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6954368.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            complement(4776..5885)
                     /gene="rpoD"
                     /locus_tag="VBD11_001422"
     CDS             complement(4776..5885)
                     /gene="rpoD"
                     /locus_tag="VBD11_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6954369.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            complement(5893..7767)
                     /gene="dnaG"
                     /locus_tag="VBD11_001423"
     CDS             complement(5893..7767)
                     /gene="dnaG"
                     /locus_tag="VBD11_001423"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6954370.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME
                     "
     gene            7891..8268
                     /gene="mscL"
                     /locus_tag="VBD11_001424"
     CDS             7891..8268
                     /gene="mscL"
                     /locus_tag="VBD11_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6954371.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT         2601 a         1647 c         1238 g         2798 t
ORIGIN      
        1 gctgtaatat ctactttttt atctgttttt acttttttat tattttggca acctactaaa
       61 aaaagcatga aaaacagaca aataaaaata tgttttttac tcatctaaag tgctatcctc
      121 tatctctttt ttcaacaaag aaacctcttg aatcaggcgt ttaatttgct catctctttt
      181 ggtattgcta ctagtatcaa ataattctaa acaaaacaga aataaaatag caccaaataa
      241 caagaaattc atcgttaaag cgaatccaag gtgatgagcg ataaatgaga ccaccccagg
      301 aaatacacta ataaagacta gaattaaacc aactaatagc caaggtaaag aacgtttaac
      361 agaaaatgtg tttttattaa ccattcttat aatcatataa agtaggacaa tggctaaaat
      421 aaacatttca atttgcaatg ctaaactcat ttatcatcct ccttaataaa ccctgcaata
      481 ataattgatg ttaaaacatc cgtcatataa aaaatcgatt ttagggaact tatacttgaa
      541 gtacctgcct cacgttcaaa catattagct gtgacttcct tcaaacgata gccttttttc
      601 aaaatccgag cagttgattc aggctctggg tattgtactg gataatgaca cgataaataa
      661 gctattagcg ccgcgttggc tgccctataa ccacttgttg tatcatagac tttgtgtccc
      721 gtcgttaatt ttatagcagc agatattaaa tttatgccaa gacgcctcat tgctgtcgat
      781 tgaaaatttt gatgtttttt atcaacaaag cgagaaccaa taacaaaatc tgcttcgtca
      841 tttaagattg gcataacaac atcttcaatg cttctaatat catgttgacc atcaccatca
      901 aattgaacgg caacatcgta atgattacga ttagcgtata agtatcctgt ctgcacacag
      961 ccaccaatac ctagattttg aacaaggtca atatgattta aacccaatct atctaataac
     1021 tctggagtac catcggttga cccatcgtta ataacaatat aatccagctc aaaaggtagt
     1081 gagcgagacg ccttaaaatc tacaatagtc tggactgtct ttgcaatact accttcttca
     1141 ttataagcag gtatcactag taaaattttt ttcatcatct tatctcttat tgtaataatt
     1201 tgttgcaatc aaccatttaa acacaacttg atgcttaaat tgatttttgc tataaccata
     1261 tttccataac caccttaatc ttgtcataaa aggctgtttg tcaattttta taaaacattt
     1321 aatgagctca agatcattag ctgtcataat atcaccatac ttatcaacaa ttaaggaagc
     1381 ttgcttttgt gagtcatgga taagtttcca ataccttgta aatatagatt taggaccttc
     1441 acgaagtatc ttaaatctct tatccattgt cctcgccccc aaaacattat tgtcatgttg
     1501 acggtatagt tgagttggta aatccaaata aatgatttct cctaaacttg ctgctaagag
     1561 tgctaaaaac caatcatgca ttaagatatc attaggagta aaccacttct cagctaatgc
     1621 atgatttatc atcatagtcc caccagtaac agtattctca gttagttctg gtaataatgt
     1681 cgtattggca tggtgcgatt gagcccgaat catgctgtct tgtaaaatat ttaattcttg
     1741 attaacaact ttcaaatcag tataaacaag taatggtaca tcagatgctt tatgctttgc
     1801 tgcctctaaa cttacagata acttttctgg caaccataca tcatcttgat cactaaaaaa
     1861 atagaaatct gcaacttcgt aattaactag cgtaaaaaat gatttaataa ctcctaaatt
     1921 atggtgctca ttttcattaa tgaaacgaat tcgagaatct ttagcagtaa agtttctaat
     1981 aatatcacat gtcttgtcac ttgaaccatc atctctgatt aggaggttcc actctttaaa
     2041 ggtttgcttt tggatacttt cgatttgctg cgccagaaac ttctctccat tgtaggtggc
     2101 cattaaaata ttaactttca tttaaaaaca aatcctcata ttcttcaaca atttttttcc
     2161 aagtatagtt ttcttgaata atggcttttg ctcgatcacc gtaagtagat aaatcctgtt
     2221 gttgatcact atcgttaata agcttagcta aatccccctc ttttttagtc cagtaaaaac
     2281 ttgagagacc tgctactgat ttgttgaaat caacatctag aacaagattc aaatcagtat
     2341 tagctaaagc ctcaagcagt cctggattag taccgccaac ttcatgccca tgaatataag
     2401 caaaggcttg ttgacgaaca taatccagca gatctttttc atagagcgta cctacaaact
     2461 taactctttt atcttgttga aggtttgtct ttaaggacaa cttttcaaag taggggttat
     2521 tttgatggtt acagataata actaaatcac gcttagtatc tgaagccatg aactccctaa
     2581 tagccgtttc ataattgttt tcagggacaa atcgaccaac gattagatag taacccttag
     2641 acttaatatt ccatttttta aacaactgtt tgacacgtgg atcatctgac gataatttcc
     2701 tagaattaat ctctgtaccg taagcaatga accttgtctt cacattagag taggtagatt
     2761 gaatgtaatt ttcaatacca atattatcag aaataattag gtctgcattt ttagtcatac
     2821 atttttcggc gtattttaaa taacgctgtg tgggacgaga ccactttgat cgcttccact
     2881 ctaaaccatc cggattaaca tacaataagc caccgacttt atgtatttta ttggcaaaat
     2941 gccaaataaa ggcaccaatt gtatttccta aaatataaaa aataggctct tttaaatcat
     3001 gtgtcttaac caagtcaagg gcataattaa tggccataat atcataagca atcacacgtg
     3061 ctggccctaa ttggggagga tttatagtaa aacaatcagc gtcagcaaag ttaaaatgag
     3121 tatgatgttc tttatcacta aggcatgcaa catggtattt tatgtcggaa cttttttgat
     3181 gattaatcaa ttctgaaaca aaagtttcaa aaccaccgta acgagctggc aaccctctac
     3241 ttcctataat gaaaacatct tgcatcatct tctccattct agctaaacaa tccatacaat
     3301 tataccataa attagaaaag ttagctaaaa agagcagtaa gctaaaagga ttctctagcc
     3361 ttactactct ttgttttgat cttatttttt aacttcctgc ttgtagaact cttttaaagc
     3421 atcttgccaa gtaggaatca caaatcctgt tgctttcgct ttttccaaac tcatagttga
     3481 atttaaagga cgttttgctt ttgctgggaa ttgactagaa tcaacaggct taacctccac
     3541 atctgtgtct tttaagattt caaccgcaaa atcataccat gttgtatctt ctttagcatc
     3601 atttgataaa tgatagtaac caaaatcttt ttgattttca gctaaataag tcataaactc
     3661 agccaaagta cgtgtccatg ttggtcgtcc atgttggtcg ttaacaacag ttagagtttt
     3721 gtgtgttttt gcaaggtttt gcatcgtaaa gacaaagttt ttaccatagt taccaaatac
     3781 ccacgctgtg cgaatggtat agaattttga agtatatttt tcaactaact cttcacccat
     3841 acgttttgta cgaccgtact ctgttttggg atcaggcaga tcatctactt cccattcttg
     3901 ccccactggt ttttcaccat caaacacgta gtccgtcgaa atgtaaacaa gtgttgcatc
     3961 atgtttagca gcagccttag cgacattctc tgtaccagtg acattgattg caaagtctag
     4021 ctcttttcct tcatcttctg ccgcatcaac agctgtgtaa gctgcacaat ggtaaactaa
     4081 agatggcttc acctcttcaa agactttgtc caccatctcg gcatttgtaa tatccatttc
     4141 agctacatca acagcaacat actcttgagt tcgttcgtct aaaaggtgac gtaattcact
     4201 acctaattgc ccgtttgccc ctgtaattaa aatcattcct tgctccttta aaaataagtt
     4261 taacttttaa ttcttcgttt attgtacact aaaaaaggga atatttcatc cctttactct
     4321 ttatcttatg aacgaattcc caaagcgata cgagcataac gactcatctt atcaacagac
     4381 caggctgggt accataccaa tttcacctcg gtttcagtta cctctgggac tgttttcata
     4441 acatcatgaa tttgatctgt caataaatct gctaacgggc atcccatagt cgttaaagtc
     4501 atatcaattt cagttcgacc gttatcttca aagcgaatct cataaattaa cccaaggtta
     4561 acaatatcta ttcccaattc aggatcgata accatctcca aagcttctag gatacgatct
     4621 tttatttttc caacttcttc ttcgctatat tgctttactt ctgacattat aatacctctc
     4681 tgaaaatatt aataccgtct ttaagataac tcctttgcaa atcattttta ttagaaacta
     4741 aacttcctat tctaaaaacg attatcgaga agcaatcaat cttccatgaa atctttaagt
     4801 tgcttgctac gactaggatg gcgaagtttt ctaagggctt tagcttcaat ctgacgaatg
     4861 cgctcacgag taacattaaa tactttacct acgtcctcta acgtacgcat tttaccatcg
     4921 tcaagaccaa agcgtaaacg aagaacattc tcttcacggt cagtaagagt atctaaaacc
     4981 tcatctaatt gctcacgaag aacaacacgt gtagtataat ctactggatt ttcaataact
     5041 tcgtcttcaa tgaaatcacc caaatggcta tcatcttcct caccgattgg cgtttctagc
     5101 gataccggct cttgagcaat ttttaggatt tcacgtactt tatctggtgt catatccatg
     5161 cgttctgcga tttgctcagg agttggatct tgaccaagtt cttgaagaag attacgttgt
     5221 tcacggacca atttattaat cgtttcaacc atatgaacag gaatacgaat cgtacgtgct
     5281 tgatctgcaa ttgcacgtgt tatagcttga cgaatccacc atgttgcata agttgaaaat
     5341 ttgaatccct ttgaataatc aaatttatca acagctttca tgagccccat gttaccctct
     5401 tgaattaaat caagaaattg cattccacgt ccaacataac gttttgcaat tgagacaaca
     5461 agacgaaggt ttgcttcagc taaacgttgt ttagccatta aatctccctc agcaaccgca
     5521 acagccaatt ctttttcttc ttcattcgtt aaaagaggaa caacaccaat ttccttcaag
     5581 tacatacgta ctgggtcatt gactttagca gaattacttc ctatgagctc ttcatcagtc
     5641 aattcttcag gttttggccc ttctaccaca tactttgttg aaggattgcc ttctttatct
     5701 gtgattgaaa tgccaccgtc tgtcaatcgt tctaacaaat catcaatttg atctgcatct
     5761 aaaacaaaag gaataacaag tttttctgtt acctcatcat caattgcagt accttgtttt
     5821 ttatgattgc gaataaaatc cgcaacttga acattaaaag ttgtatttcc ttttttctct
     5881 gccataggtt cctcattcca tatttctttt ttgagcaatt aaattttcta atgcagccaa
     5941 ggctccttct tcatcacctt gattactaga ttgtctaatt aactggcttt gttttcttaa
     6001 atcacgttct ttcaataaac ggtcacgtgc cttttcgacg gcttcaatct ctccaataga
     6061 tacttctaca ggtaattgtt cctctaaaat tatataataa gtacgattaa ctttgtcact
     6121 ttcttgtgat aaatcataag acgtgatctc accactttct tttagcaact catataaaac
     6181 ttgtatttct gttgtatcaa aataaaaatt atccctatta cggaattcat ttaacaggta
     6241 gggatgattc aataatcgat gaaacaattg catttctgtt ttagtgatag ctgataactt
     6301 acctgtctgt ggtaattgca ctgtagcact tgtttgtact gaagatgaag cttgctgttg
     6361 acttctatga tgcaatcgtt cgttattaac cgattgttct acttgaaaat aatcaaaatc
     6421 tggcaagagt tcagccactt ttgtaatata tgaattttgt gctgttatcg atggagattt
     6481 agcaattaat ttcgctattt tttcaacata agcaatttca gattgtaaat tatcggtgtt
     6541 ttcaggcttt aagtagtgga tataaaactc tgtattactg atgcgcccat tttctaagag
     6601 ttgtttaaaa tcttctgctg aatttgcttg tagaaattca tcagggtcca ttttattagg
     6661 tatcctaacg atatcaacag tcatatctga tagcagttct aaagacttgt caattgcgtt
     6721 ttgaccagct ctatcaccat cataagtcag aacaactttc ttagtaaatc ttttaaggtg
     6781 tctaacgtgt tcattggtta aagcggtccc catagaagca acaacattct caattcctgc
     6841 acgataagct gcaataacat ccataaagcc ttccattagg taaacttcgt gtgctttatt
     6901 aattacagca cgcgctttat ctaaatggta aagctcgtaa gacttattaa aaataggggt
     6961 ggcacgagaa tttttatatt tagcttgttt tctatcaatg tcctcttgag tccaaactcg
     7021 tcctgaaaac gcaatgactt caccaatatc attagttaac ggaaacataa ttctattatt
     7081 aaatgaatcg taaaactgat tgctattttc tgaataattg aacaaaccag aagacatcaa
     7141 agttttttca tcaaactctt ttgccagacg acgatataga aaatcctgcc ctcccggcga
     7201 taatcctatt tgaaaatgtt taattaagtc ctctgtcact ccgcgctcgg caaggtattg
     7261 tctagcgcgt tcaccatcat tagttgtcat taaataggca tggtaaaatc ccgaagcaac
     7321 tctatgaata tctaaaagcg actgattagc tttaggtttt ttaggaactt gccccttaaa
     7381 attagtatct agaggtattc ctgaacgttc agctagaact tgtacactat ctaaaaaact
     7441 aatatgttgg taatcttcca caaatttaaa gacatcacca gagcgaccac aaccaaaaca
     7501 atgaaaaaac tgcctatctt cgataacatt aaaagatggc gttttttctt tatgaaatgg
     7561 gcataaacca agatgattgc gtcctgtttt tgtcaaaccg acaacttcac cgatgacatc
     7621 aacaatgtta acactgttct tgatttcaga aattttttct ttatcaattg ccaagtcatg
     7681 acctccacag aaataaccca ttatagtttt acatttgatt atatcatttt ttcttttctg
     7741 tgtaaatttt ttcaaaagct ttttcaagga tttcttcttc actgatttaa tataaaaatt
     7801 tacagataat ttgaaaccta ttacttggat ttctcaaaag aaatctgcta taatgaacgt
     7861 gaattaaata atattaagga gcttgttcac atgattaaag aactaaaaga atttttattt
     7921 aagggtaatg ttttagacct tgctgttgct gttattttag gtgcagcttt taatgctatc
     7981 atcacttcac ttgttaaaga cgttattaca ccgcttatcc ttaatcctgt attaaaagct
     8041 gctggcgttt caaatatcgc acagctttca tggaatggtg ttgcttacgg taatttctta
     8101 agtgcagtta ttaatttctt aatcgttggt actactttat tcttcatcgt taaagctgct
     8161 aacaaggtta tggctaagaa acctgctgaa gaagaaatta ttgaagttgt tgaaccaaca
     8221 caagagcaat tacttgctga aatccgcgac ttactcgcta acaaataata aaagactcgg
     8281 atta
//
LOCUS       DATROY010000081         8145 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.082, whole genome shotgun sequence.
ACCESSION   DATROY010000081 DATROY010000000
VERSION     DATROY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(181..1608)
                     /locus_tag="VBD11_001425"
     CDS             complement(181..1608)
                     /locus_tag="VBD11_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6954372.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(1625..3493)
                     /locus_tag="VBD11_001426"
     CDS             complement(1625..3493)
                     /locus_tag="VBD11_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6954373.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(3486..4337)
                     /locus_tag="VBD11_001427"
     CDS             complement(3486..4337)
                     /locus_tag="VBD11_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6954374.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(4675..5283)
                     /locus_tag="VBD11_001428"
     CDS             complement(4675..5283)
                     /locus_tag="VBD11_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6954375.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(5363..6400)
                     /locus_tag="VBD11_001429"
     CDS             complement(5363..6400)
                     /locus_tag="VBD11_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6954376.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(6454..7182)
                     /locus_tag="VBD11_001430"
     CDS             complement(6454..7182)
                     /locus_tag="VBD11_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6954377.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            7358..>8145
                     /locus_tag="VBD11_001431"
     CDS             7358..>8145
                     /locus_tag="VBD11_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6954378.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQ"
BASE COUNT         2700 a         1583 c         1180 g         2682 t
ORIGIN      
        1 taggttaaat aagatttatg aatcaacgca actcaatcca agaaacttta aggatgcaac
       61 tttattgtat ctagcactta aatcagaaga tatttacctt cataaattat aataaaaagg
      121 agtaacctat ggatactcct ttttatatta aattgaaaca gagactagtt gtttaacatg
      181 ttatctgtta tgatgttcaa acaatgattg gccatttgtc ttaatcactt tttggtacca
      241 accaaatgac ttcttcttgt aacgttgtag actccctgtt ccatcatcat ttcgatcaac
      301 atatattaga ccataacgtt tacttagttg agctgtagac atggatacac aatcaatgca
      361 tccccaagat gtatatccca ttatttcaac accatcttca atagctttag caacttgaac
      421 taaatgttga ttcatataat caatacgata atcatcttcg acagtataat caccgtctag
      481 tgtttctatc aattgatctt ttgctcccaa tccattttct acaataaaca acggaatctg
      541 ataacgttca taatactgat ttaagactag gcgtagccct atgggatcga tttgccatac
      601 ccattctgaa gttgttaaat aaggattagt caggcctcca aggatatttc cctgaccact
      661 ttgataattt tcaaagtcat aagcttgtgt cacgctcata tagtaactaa acgataggaa
      721 atctactgta ttttgtgcca atacttcctc atctccttct tcaaatttaa tttcaatacc
      781 attatttttg aaataggact gaatataagt gggatattta cctctgacat gtatatctga
      841 aaatagcaga ttatgttgtt caaattgtct tgcagccaga acatctcttg gatcagatgt
      901 cataggataa gctggcatag ccagaatcat acagccaatt ttaaaattag ggttgataga
      961 acgccctaat tttgtcactc tagccgatgc tactaattca tgatgaatag cttgataaag
     1021 ctcctgagga cttagttgtg atttgtcggc cataatagca ccactggtga aaggcatatg
     1081 taaaatagaa ttcacttcat taaaagttaa ccaatatttt accttatctt tataacgctc
     1141 catgactgtt tgagcaaatt tctcaaaaaa tgctatcaaa cgtctatcag cccaaccatt
     1201 ataggtcttt gcaagatgaa gcggtgtttc ataatgagat aaggtaacca aaggctcgat
     1261 attatattta agcaactcgt caaataaatt atcataaaat tgtaagccag cttcgtttgg
     1321 agcactatca tcaccatttg gaaaaattcg agaccaggca atagaggttc taaaaacttt
     1381 aaagcccatc tctgcaaaca gttttatatc atttttatag ttatgataaa aatcaattgc
     1441 ttctagcttc aaattgtctg gagtaggttt ggcggtaaaa tcgcctagac caccatttgg
     1501 aagtacatct tgtacagata aacctttgcc atctgttcta aatgcaccct ctacctgatt
     1561 ggcagcaact gcacctcccc atagaaaatg tttaggaaaa atcgtcatat tatcatcctt
     1621 ccttttaaag aattacaagt aaatcatctt catttgtaac cattgtctct tcgtctttaa
     1681 cgataatttc aagttggtca attgaatttg taactacaac tggactaata agagaatacc
     1741 cctttgaact aataaaatca atatccattc gcaaaagtaa ctgtcctcgt ttaacaaaat
     1801 cgccaacttt aaccaattgt tcaaaacctt ctccatttag ttctacagta tctattccaa
     1861 tatggattaa cacttcaact cccttatcat cctttaaggc aatggcatgt tttgtaggaa
     1921 ataaacttat aatttcacca tcaaatggtg cataaacttc acccacacta ggagtgattg
     1981 ctaaaccttc acctagtaat ttagatgaaa atgtttcatc gggtacttta gataaaggta
     2041 atacattgcc taccataggt gaatacaatg tctctttttt tgataaacca cttctaatat
     2101 gtgtgtactc ttgagcatta atactacttt tttcgttttc tccttcatca attcccaaga
     2161 accatgtaat aatgaaagtc aaaataatag tagctattgc tacaataaca gcattactga
     2221 aattattgcc cccctgtgga ttgatgtatt gtggcaaacc aataatagat ggcactacaa
     2281 atgtatagga agcaatatta actaatccaa tataagctcc tactaaacca cctgatatca
     2341 ttgcagcgta taaaggcttc ttgaatttca gtgtgacacc ataaagtgca ggttctgtga
     2401 taccagctag tagtgctgat aaacctgctg caaaggccac ctgacgtgtc tgtttttgtt
     2461 tagccttaac tgcaacagca agggaagctg caccttgagc taaattagaa gctaacattg
     2521 ctggtaaaat caaaacatct ggtgtcgtaa cagaagcagc taaaaagatt ggagcaaatg
     2581 cccagtgcat tcctgtcata acaaccaatg gcataatagc accaagaata gctaaagcta
     2641 accatggtgc cacatggtaa atcgaaagca ttgcactaga caatccttta ccaataatta
     2701 ctcccagtgg ccccacgacc actaaggcta aaaagccaga aatgagaata atcaaagtcg
     2761 gttgtaaaaa gctcttcatt acactaggaa caattttatt aatccatctc tcaatgtatt
     2821 gcataagaaa aaccataatt aagattggaa taacagaaga accataacta gccagtgtaa
     2881 ccggagctcc aaagaaagat aatggttttc cttcagctac cattgtcaca aaagcagggt
     2941 gtaagagaat ccctcctact gtagcagcta atacaggtgt aactttaaat cgtgacgcag
     3001 ctgaataagc aatcattatt ggcataaaat aataagcggc atcaccaaaa aaattgataa
     3061 aagctactgt ttgagaacta gagcttgtta taccgagcat agggagcaaa atacctataa
     3121 ccttgagcat acctcctcct agaagagcag gtataagcgg cttaatgact cctgcaatcg
     3181 tttcaattaa tttctcaaat attgaactct ttttttgtga tgaaaattct ctggtagtat
     3241 tttcaaaatg acctaaggcc aaaaaagcat tataataatt attgacatca tttcccaaaa
     3301 taatttggta ttgatcattt ttacgcataa caccgataac attcggaata gttttaataa
     3361 cctgatcgtt gacaatttca tcattgtcta aaactaatcg taatcgtgtc acacagtggg
     3421 ttacgtgttg aatatttttt tctcccccta ctgctgctaa aatagcttta gcagtctctt
     3481 ggtatttagt catctgatat ctcctttaaa tttttaacta atcgtttaac atgcaccgtc
     3541 agatatagga gttcacttga attaagttga tattggtatt ggatagccat attgttgccg
     3601 atctctttaa cacatttata ttctctcgga tattttttct taactaccaa aagtaaatca
     3661 gcatcatcat cttcgtattt cattcctgat aaaacgcgtt gagcaaataa tttggtatgt
     3721 gtcatgaaac gataataatc aattgaactt tcatctagtt ctgttcgaaa atctattttt
     3781 actttctgtt caatgtaaga tacaatctca gtgattttat gcgcttcatt aaacggatta
     3841 tccaatccag catttacaaa gtgcatagca ataaaagctg actcatcaat tgttaaacaa
     3901 atacccaatt catcctttat aagttccaga gccttcatcc caatggagta ttcatcagga
     3961 tagtaacgct ggatttccaa ccttagtgga ttttggataa ccattctttg ttcatgtctt
     4021 tcaatagccg aatgaatatg atctgtcaaa ttgatataaa gaatttcatc taagttctta
     4081 cccaatttga ttttacctag gttgataatt cgttctgaac atgcaactac ttcttctgga
     4141 acagtaataa ataactccgt aaaacggttc atgttatcac tattttttaa aacaaatgac
     4201 ttctcaatag catcgctatt aatcctatca cctattcttt tcttaaatgc aattcctttc
     4261 cccatcagta acacatctaa tccttgatgt gttactgata tgactgcatt atgatttaat
     4321 acgcgtttta taatcattaa agtcctagtc cttcactcgc acaataaaaa atgacccatc
     4381 caagacacaa caaaaagtcg tcgttgaata ggtccctgct cacaaaatgt gataacatcc
     4441 tttattatgc aattttatct gttatcagct tgcttacctt aaataataac acctgaaaac
     4501 gtcttaattt tatcatttct tactaatctt gtcaagcgct ttcaataaac ttattcgctt
     4561 aattgaagat aataggactg ttctagttct atctgagttt ggatttctag acttttaaaa
     4621 acattatcaa cttttagaat aataaaaaaa cgagcctatc gactcgtttt tttattaagc
     4681 tttagcagct tggtaaagtt catttacttt attccagtta ataatttcaa agaaagcttt
     4741 gatgtagtta ggacgaacat tacgatagtt taagtaataa gcatgctccc atacatcaag
     4801 tcctaaaata ggtttcttac cttccataat tggagtatct tgattggcag ttgaaagcac
     4861 ttcaagtttg ccttcagcat taacaacaag ccaagcccaa cctgaaccaa aacgtcctgt
     4921 tgctgctgct gtgaaagcag ctttaaagtc ttcaaatgaa ccaaaagttg catcaatgtc
     4981 ttcagataac tcttgtgaaa tttgagtttc ttctggtgac atcaattccc agaaaagagc
     5041 gtggttaaga tgtccaccac cgttattgat gactgcctga cgaatatctt ctggaatttg
     5101 agaaacatca gctaagagcg cctctaagtc ttctccaatt tcaggatgtt tctcaagagc
     5161 agcatttgca ttagcaacat aagttgcatg gtgcttatca tgatgtagtg tcattgtctc
     5221 agcatcaata tgtggctcaa gcgcatcata tgcatagggt aaatctggta aaataattgc
     5281 catgtcgaaa tcctcttttc atctattgtt acccttattc tataggaatt tggaacagct
     5341 ttcaactctt ttgtttcttt aattagggct ttttatgagc aatttttaat aaggctaaat
     5401 caaagagata atccttatca taagtccctc ttttaatagc atagtcagtc tcgattaaaa
     5461 tcctaatcgc ttcttttaaa aaagcaaggg gaaggtttct agaatctctg acagcaaatt
     5521 ttacttgata agggttgatt ttacgtccta tgtaatggga tagttcagaa acaatttgac
     5581 tctctgactt tcctttacta gccaatattt taacttgcaa aaacatccga aactgtccta
     5641 acatgattgc aatcaatttg atttcgtctt cgccttgtag acgtaaatct cttactaaat
     5701 cacgcgctaa atctatccta ccaagtaaga catcttgagt caaatcaaat atattatctt
     5761 gcaggctttt agggatagcc tctctaacat cattagacga aatatgaccg tctgttttat
     5821 atgactttaa gaaagcgata ttagttaaag tatcactaaa atcatagtta gacttaatca
     5881 ataactcatc aaaaacacct gcttcaaaaa ctaatccttc ttggtgagca tatttttgaa
     5941 aatacgtctt taaatctgat tcttttaaag tattagcctc taaaacacga gcatcacgtt
     6001 tcaataactt tacaagtcga cgttttccat caagttttcc tggagcacaa ataacaagtc
     6061 tcgtagtatc tacaggattc tgcaagtagg cttccaagcg cttcattgct tgttcatcta
     6121 aataagtttt tttatcagtt gtaatatctt gaaactgatc aaaaataaca accttgtagt
     6181 ctgataaaaa aggtaaactt tctaagtcta attccgcatt ttgatattct tcttcactta
     6241 aatcaaaata agaataggct aaatcgtctt tattgaatcc aattacctga aatagcttct
     6301 ctttcatctg agcatattgc cctaaatctt ctcctgctaa aacagttact aaccctaaat
     6361 tatctggggt aattctccca atttcttcaa tcgcaatcat attccttatt ataccataag
     6421 gaaacttttc aaggtgtcaa ataatactta aaatcattca tcaactctat gagaaataat
     6481 tccaaattga caatctcgga taaaatggtc attattctct tctaattctt tttgtaaatt
     6541 gacatcttct aacccctgaa ggtcactata tttttgtatt ctttctttca tgatatttag
     6601 tagccaagat agatctgtac cagtttgtga cgtttgaatg acattttctg atacaaaatg
     6661 gtcaacgatt aagcctgctt ctaacattaa ttgctcaagt tgacttccaa tatgaatgtg
     6721 tcctccttcc ttctcaacag tttgccagat tagtgattgt attttattca tatttacaaa
     6781 gctaggctta gctagttcta aacacattgc atcactttct tgaaaaacat aaaaaccatt
     6841 ttgctttaac aaaggtgtta aactttctat tactttcttt ggattttttt gatacattaa
     6901 tacacgacgt ccaataatag catcaaaact ttttgtttca aaggaggaaa gtgctgctaa
     6961 atctgcattt tgataactaa cattagcata gccattatca tgtctagctt gccataaaag
     7021 ttgctcatta atatcaattc ctacaacatc accttcttta ccaacgatat cagcagccaa
     7081 acgcgttaat tccccactac cacaaccaat atccataact ctcataccgg gctgcaaagc
     7141 tttttttaat aatgcttttg aaaaatcttc tctgtgaaac ataataacac tctcctctct
     7201 tctattttag caaattaaag gttcatacgt actatcagca aaaaattata ataggttgaa
     7261 attttatagc attattttct tgttttcgta tctactttat tatagaataa caactaatca
     7321 tttttagcat gacgagctca attacaaaag gataatcatg aaaaactatc gaaaacttat
     7381 tgtactacta cttctaatct tttttgccat ttttatggga gcatatgctt acacgcatat
     7441 tgttgaaaaa agatccctaa ctagcaatac tattgaaaaa actctacctg tggtaaatca
     7501 gattaagcct caaaccatta aagaatacca aaattactta actaaggtag ctaaacgtaa
     7561 tgttcttcct gtagacattc ctcaggcatt aaataatgaa aaggtagaaa ttactgctac
     7621 tgatggcatg caaacattca cttggaatga taaaaataat cctaagcaaa aggttatctt
     7681 ctatgttcat ggaggatcat atatccatca agcttccgaa ttacaatata tttttgtcaa
     7741 taaactagct aaaaaattag atgcaaaagt tgtctttcct atttacccta aagctcctac
     7801 atataattat agtgatgcta tccccaaaat taaaaaatta taccaaaata cattagctag
     7861 cgtcacatct cacaaacaga ttatcctagt aggtgaaagt gcaggcggag gccttgcttt
     7921 aggtattgct gataaccttg tcacggagca tatcaaacaa ccaaaagaaa ttattttaat
     7981 atcaccatgg ttagatattg ctactaataa tccaaagatt gaaaaagttc aaaaaaaaga
     8041 tccccttctt aaagcttggc aattacaaca agtagctcct tactgggcaa atggaaaaaa
     8101 gaattttaaa aatcctcaag ttagtcctct ttacagtagc caatt
//
LOCUS       DATROY010000082         7981 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.083, whole genome shotgun sequence.
ACCESSION   DATROY010000082 DATROY010000000
VERSION     DATROY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            50..976
                     /locus_tag="VBD11_001432"
     CDS             50..976
                     /locus_tag="VBD11_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6954379.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            1147..1863
                     /locus_tag="VBD11_001433"
     CDS             1147..1863
                     /locus_tag="VBD11_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6954380.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            1865..2698
                     /locus_tag="VBD11_001434"
     CDS             1865..2698
                     /locus_tag="VBD11_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6954381.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(2750..3553)
                     /locus_tag="VBD11_001435"
     CDS             complement(2750..3553)
                     /locus_tag="VBD11_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6954382.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            3719..6169
                     /locus_tag="VBD11_001436"
     CDS             3719..6169
                     /locus_tag="VBD11_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6954383.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNEPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            6251..7966
                     /gene="pabB"
                     /locus_tag="VBD11_001437"
     CDS             6251..7966
                     /gene="pabB"
                     /locus_tag="VBD11_001437"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6954384.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         2611 a         1199 c         1576 g         2595 t
ORIGIN      
        1 taacttaatt taatacataa cttatattaa ttttaaaagg agcattttga tgaaaaagtg
       61 gttagttatt gtatcatgtt ttgtagcatt tttaggctta ggtgcctgtg ctaacaagca
      121 ggatcaagcc aaaaacgaaa aattaaaagt agttgtaacc aactctattt tggcagatat
      181 aacaaaaaat atagccggta acaaaatcga cttacatagt attgtacctg ttggtaaaga
      241 tcctcatgaa tatgagccat taccagcaga cgttgaaaaa acaactcaag cagaccttgt
      301 tttttacaac ggaattaatc ttgaaacagg cggtaatgct tggtttacaa aattaataaa
      361 gaacgctaag aagaaagaaa acaaagatta ctttgcggtt agcgatggag ttgatgttat
      421 ttatctaaat ggtcaaagcg gaaaaggtaa agaagaccca catgcttggt tgaaccttga
      481 aaatggtatt atttactcaa aaaatattgc taagcaatta attgctaaag atccaaaaaa
      541 caaagctact tatgagaaaa acagagatgc ttatgtagct aaattagaaa aattggataa
      601 agaagcaaaa tctaaattta atgccattcc agctaataaa aaattaattg ttactagtga
      661 aggttgcttt aaatatttct caaaagctta tggagttcca tcagcataca tttgggaaat
      721 taacactgaa gaagaaggta caccagatca aattactagc ttagttaaga aattaaaaca
      781 agtaagacct tcagctttat tcgttgaatc aagtgttgac aagcgtccta tgaaatctgt
      841 ttcaagagaa tcaggtatcc caatttacgc agaaatcttc acagactcaa ttgctaaaaa
      901 aggtcaaaag ggtgacagct actacgctat gatgaaatgg aatcttgata agattgcaga
      961 aggtttagca aaataagagt gagaatcgac attgaaatca taatgaaatc aatgatgtag
     1021 tattctcgct taaggctgag ttgtaacaat gtcgaaatcc taatctaaaa actgacttag
     1081 ctttaataaa tacagagaag tggagtctgg ttttataacc agctccattc tacttttgga
     1141 ggaaaaatga ttattagtaa acacttaagc gtaagttatg ataataattt agtgctagaa
     1201 gatattaatt tacgcttaga aggctccgga atcattggta ttttagggcc taatggggcg
     1261 gggaaatcta cgcttatgaa agctcttctt ggtttggttg attctactgg agaatcaggg
     1321 attgggggag atttgctacc tttaatgggg agagtagcat atgttgaaca aaaaactaat
     1381 attgattacc aattccctat aactgttgga gaatgcgttt ctttaggatt atataaggaa
     1441 cgaggcctct tcaagcgact atcaaaaact gactgggaaa aagtatcccg agtgattgat
     1501 caagttggtt tgagagggtt tgaaaataga ccaattaatg ccttatcagg tggacaattt
     1561 caacgtatgc ttatggcaag atgtctcgtt caagaagcgg attacatttt tttagatgag
     1621 ccgtttgtag ggattgattc cattagtgaa caaatcatag ttaatttact aaaaaaatta
     1681 agtaaggccg gaaaattaat tttagtcgtc catcacgatt tgagtaaagt tgatcattat
     1741 tttgatcaag taattatttt gaaccgtcat ctaattgctt gtggaccaat agatcaggca
     1801 tttacccgtg agaatttaag tgctgcttac ggtgatgcta ttttgttagg acaaggagac
     1861 tagaatgttt acgaaatttt ttgaaggctt attgacttat cattttttgc aaaatgcttt
     1921 tataactgca attgtgattg gtattgtagc gggtgcagta ggctgtttta ttatcttgcg
     1981 ctcaatgtct ttgatgggtg atgctatttc tcacgctgtt ctaccaggtg ttgctatatc
     2041 ttttatttta gggattaact tttttatagg tgctattgtc tttggtttgt tgtcttcgat
     2101 tatcattact tatattaaag aaaatagtgt cataaaagga gatactgcta ttggaattac
     2161 tttttcgtcc tttttagcct taggtatcat tttaattgga cttgctaata gtacaactga
     2221 cctctttcat attctttttg ggaatatttt ggcagtacaa gatagtgata aatatatgac
     2281 tattattgtg ggattaattg tactgactct gattactatc ttctttaaag aattattatt
     2341 gacttctttt gatcctgttt tagcaaaatc aatgggaatg agagtttctt tttatcatta
     2401 tttattgatg attttgttaa cattagttgc agtaacagct atgcagagtg tgggaacaat
     2461 attgatcgta gcacttttaa ttacaccagc tgcaacagca tatctttatg ttaaaagtct
     2521 cagaacaatg ttgtttttat catctgcatt aggagcagta gcatctgttc tggggttata
     2581 tatcggttat acatttaata ttgccgcagg atcaagtatt gttttaacat ctacttttat
     2641 gtttttactt gctttcctat tttctcctaa acaaagtttg tttaaaaaga aacaatagtg
     2701 ttgttaaaaa atgagactag aaaatgttct agtctcattt ttgatagaat tatttaaatt
     2761 tgtagtcttt aataactttg atacttgtaa tagtaatttt ttctttcggt tgatctgatt
     2821 ttgtaacttc taccgaagca attttatcca cagtttccat gccagagatt acttgtccaa
     2881 agacggtata accaccatct aaacttggat ttcctccctc tgaataggct ttaataatca
     2941 ccttaggaac ttttttatct gacagttgtt tggaatgatc ttgctggctt tgattgataa
     3001 agaattggct gccattagta tcagctccag catttgccat tgctaaactt cccctgatat
     3061 tgtagaggta aggggatatt tcattaacaa aaccatttcc agaatctttc tttttatctt
     3121 tgctattcca tatagatttt ccaccggtcc cgtctccatt agggtcacca gattgtatca
     3181 taaaatcttt gataactctg tggaaactta acccattata gtatccttct tttgcatgtg
     3241 ttagaaaatt ttcaacagct aaaggagcat attttggaaa taacttgata tttatatcac
     3301 ctttagaggt ttttataagc acttgtgcct cgtttttggc aacagccttg tccaattgag
     3361 gaaaagcttt tttatcagct tttaaggctt gcttagtttt tttgtttagt tgttcagtag
     3421 cttcctttga agagttttca gctagttttt ggtcaacata acggtctcct tttagagaac
     3481 gctcaattga ctcgcaacca cttaaagtta atatactgac acaggctaaa ccaagataaa
     3541 taattttttt catacatact ccttgattaa gttattaaga tgattatatt ttatcataaa
     3601 tttttaaata atttataaaa gtaaatagta aacctattgg gggacttgtt gaaattatga
     3661 tagaatagta tagcatgagt tgatagagaa agtgaggaac aagtatttca cttgaaatat
     3721 ggtatttatg gcaaataaga aaaaaacaaa aggaaagaaa accagaagac ctactaaggc
     3781 agaaatagag cgtcaaagag ctattcaaag gatgattact gctcttgttt taacaattat
     3841 tctcttcttt ggtattatca gattaggtat ttttggtatt acagtctata acgtcatccg
     3901 ttttatggta ggtagcttgg cttacttatt tattgcggca actttaatct acctttattt
     3961 ctttaaatgg ttgcgaaaga aagatagctt agtagcaggt tttttgatag cttctttagg
     4021 attattgatt gagtggcatg cttacctttt ctcaatgcct attttgaaag ataaagaaat
     4081 tttgcgttca actgctcgat taattgtgtc tgatttaatg caatttaaaa tcactgtttt
     4141 tgccggtgga ggtatgttgg gtgctttgat ttacaagcca attgcttttc tcttttctaa
     4201 tattggtgcc tatatgattg gtgttctctt catcattttg ggtctctttt taatgagttc
     4261 tctggaagtt tatgacatcg tcgaatttat tagagctttt aaaaataaag tggcagagaa
     4321 gcacgagcaa aataaaaagg agcgttttgc taagcgagag atgaaaaaag caatcgctga
     4381 acaagagcgc atagagcgtc aaaaagctga agaagaagct tatttagctt cggttaatgt
     4441 agaccctgaa acgggtgaga ttctagagga tcaagctgag gacaatttgg atgatgcgct
     4501 accacctgag gtaagtgaaa catcaactcc ggtatttgag ccagagatcc ttgcttatga
     4561 gacatcgcct caaaatgaac ctttaccagt agagccgaca atttatttag aagactatga
     4621 ttcgccgatt cctaatatga gagaaaatga tgaggaaatg gtttatgatt tagatgatga
     4681 tgtagatgat agtgatatag aaaatgtcga ctttacacct aaaacgacac tggtttataa
     4741 attaccaacg atagatttat ttgcaccaga taagcctaaa aatcaatcca aagaaaagga
     4801 tttagtccga aagaatatca gagttttaga agaaacattt agaagttttg gtatcgatgt
     4861 aaaagtagaa cgtgctgaaa ttggaccatc agttactaaa tatgaaatta aaccagcagt
     4921 tggagttcgt gtgaatcgta tttcaaatct atctgacgac ctagctcttg ctcttgcagc
     4981 aaaagatgtg cgtatagaag caccaattcc tggaaaatca ttaataggta ttgaagttcc
     5041 taactcagaa attgcaacgg tttctttccg cgaactttgg gaacaatctg atgccaatcc
     5101 tgaaaacctt ttagaagtac cactaggaaa agctgttaac ggcaatgctc gcagttttaa
     5161 cttagctaga atgccgcatc ttttggtagc tggttcaact ggttcaggta aatctgtggc
     5221 agttaatgga attatttcaa gtattttgat gaaggcacgt ccagatcaag ttaagtttat
     5281 gatgattgat cccaaaatgg ttgaattatc tgtttataat gatattccac atttattaat
     5341 ccctgttgta accaatccgc gtaaagcaag taaggcactc caaaaagttg ttgatgaaat
     5401 ggaaaatcga tacgagttat ttagcaaaat tggtgtgcgt aatatagcag gttataatac
     5461 aaaggttgaa gagtttaatg cttcctctga gcaaaaacaa atacctttgc ctttaatcgt
     5521 tgtcattgta gatgaattgg ctgacttgat gatggttgct agtaaagaag ttgaagatgc
     5581 tattattcgt ttggggcaaa aagcacgtgc tgcaggtatc catatgattc ttgcaactca
     5641 acgtccatcc gtagatgtta tttctggttt gattaaagca aatgttccgt cgcgtattgc
     5701 atttgctgtt tcaagtggta ctgatagccg tacgatcctt gatgaaaatg gtgctgaaaa
     5761 gctcttggga cggggtgaca tgctctttaa gcctattgat gagaatcatc cagtacgact
     5821 acaaggttcc tttatttcag atgatgatgt tgaaaggatc gttggtttta tcaaagacca
     5881 agccgaggct gactatgatg atgcctttga tcctggagaa gtatctgaaa cagataacgg
     5941 ctctggtggt ggcggcggag tacctgaaag tgatcctctt tttgaagaag ccaagggact
     6001 cgttttagag acgcaaaaag caagtgcctc aatgattcaa cgccgattgt ctgttggttt
     6061 caatagagca acaagactaa tggaagaatt agaagcagcg ggggttattg gtccagcaga
     6121 aggaaccaag ccacgaaaag ttttaatgac tccaactccg agtgaataag aaaaaactaa
     6181 tctgacaaga aattgtcgga ttagtttttt atgttataat gaagcaaata gatgaagaag
     6241 aaggttacga atgcatattg agactgttat tgatttcaaa gaattaggaa aaagatatcg
     6301 ttttaaaaat cctacaaaag aattaatagc tgatacttta gaacaagtct tagaagtgat
     6361 aaaagaagtt gattattatc aatctcaaaa ttattatgtt gttggttatt tatcttatga
     6421 agcatctgct gcttttgatt cacattttaa agtttctcaa cagaagttgg ctggagaaca
     6481 tctagcttat tttacagtac ataaagattg tgagaacgaa gcttttcctt taagttatga
     6541 aaatgttaga ttagcagata attggactgc taatgtttct gagcaagaat atcaagaggc
     6601 aattgctaat attaaaggac aaattagaca aggaaatact tatcaagtaa attatacact
     6661 agagcttagc caacaattat gctcggatcc ctttagtgtt tatgaacgcc taatggtaga
     6721 acaaggagca ggatataatg cttatattgc ctacgacgat aaaaggattt tatctgtaag
     6781 cccggaattg ttctttaaga agaaggatga agtcttaaca actagaccaa tgaagggaac
     6841 aagcgctaga aagcctactt atcaagaaga tgtggcggag cgagattggt tggcaaatga
     6901 tcctaaaaat cgttctgaaa atatgatgat tgttgattta ttaagaaacg atatgggccg
     6961 tatctgtgat gttggaacag ttaaggtaaa aaaactatgt caagtggagc agtatgcaac
     7021 tgtgtggcaa atgacatcaa ctattgaagg agttttatcg ccagaagtga cacttatgtc
     7081 tatttttcaa gctctatatc cttgtggatc tatcactgga gctcctaaga tatcaacaat
     7141 ggctattatt aatgagttgg aaaaacggcc aagaggtatt tattgtggga cgataggact
     7201 ttgcatgcca gacggacaag ctatttttaa cgttcctatt cgcacagtac aaatgaaagg
     7261 tcaacaagcc tattacggcg ttggtggagg tattacgtgg gagagccaga cagattctga
     7321 atatgaagaa acccgtcaaa aatcagctgt tttaacacgt gtcaatccaa aatttcaatt
     7381 aataactaca ggaagagtga ctgaaaataa acttcttttc tctcaacaac atgtcgagag
     7441 attagttgaa tcagcgtctt attttgctta ttcttttgat aaaagtaaat tcgaaaggga
     7501 actgaaaaaa taccttcatc agctagatga gaaggattac cgcttgaaaa taatgcttga
     7561 taaaactggt aaggtaacgt ttgaggtaaa acaactagtg aatttatcaa aaaagttttt
     7621 aacggcagag gtggtcgtac aagactaccc tattaaatta tccccgttta cttattttaa
     7681 aacttcttat cgcccacata ttattgaagg tcagaatgaa aagatatttg tatctcctga
     7741 ggggttgcta ttggaaacaa gtattgggaa tattgtttta gaaaaaaatg gaaggttttt
     7801 aaccccagat ttatcagaag gagggttgaa tgggatttat cgtcgtcatc tccttaaaaa
     7861 ccaaaaagta attgaagcac cactaacttt aaaagattta gaatcagccg atgctatata
     7921 cgcctgtaat gctgttagag ggctttatcc tctaaaccta aagtaaaaag agttggaaat
     7981 t
//
LOCUS       DATROY010000083         7956 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.084, whole genome shotgun sequence.
ACCESSION   DATROY010000083 DATROY010000000
VERSION     DATROY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..782)
                     /locus_tag="VBD11_001438"
     CDS             complement(<1..782)
                     /locus_tag="VBD11_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6954385.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /locus_tag="VBD11_001439"
     CDS             complement(782..2125)
                     /locus_tag="VBD11_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6954386.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /locus_tag="VBD11_001440"
     CDS             2265..3116
                     /locus_tag="VBD11_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6954387.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="VBD11_001441"
     CDS             3119..4078
                     /locus_tag="VBD11_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6954388.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /locus_tag="VBD11_001442"
     CDS             4132..5484
                     /locus_tag="VBD11_001442"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6954389.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="VBD11_001443"
     CDS             5607..5978
                     /locus_tag="VBD11_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6954390.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="VBD11_001444"
     CDS             6003..6383
                     /locus_tag="VBD11_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954391.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /locus_tag="VBD11_001445"
     CDS             6476..7606
                     /locus_tag="VBD11_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6954392.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..>7956
                     /locus_tag="VBD11_001446"
     CDS             7610..>7956
                     /locus_tag="VBD11_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6954393.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMD"
BASE COUNT         2613 a         1251 c         1575 g         2517 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcacaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cgatttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattt
//
LOCUS       DATROY010000084         7844 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.085, whole genome shotgun sequence.
ACCESSION   DATROY010000084 DATROY010000000
VERSION     DATROY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..278)
                     /locus_tag="VBD11_001447"
     CDS             complement(<1..278)
                     /locus_tag="VBD11_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954394.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQR"
     gene            460..1446
                     /locus_tag="VBD11_001448"
     CDS             460..1446
                     /locus_tag="VBD11_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6954395.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            1439..2350
                     /gene="rbsK"
                     /locus_tag="VBD11_001449"
     CDS             1439..2350
                     /gene="rbsK"
                     /locus_tag="VBD11_001449"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6954396.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            2325..2723
                     /gene="rbsD"
                     /locus_tag="VBD11_001450"
     CDS             2325..2723
                     /gene="rbsD"
                     /locus_tag="VBD11_001450"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6954397.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            2739..4217
                     /locus_tag="VBD11_001451"
     CDS             2739..4217
                     /locus_tag="VBD11_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6954398.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            4219..5160
                     /gene="rbsC"
                     /locus_tag="VBD11_001452"
     CDS             4219..5160
                     /gene="rbsC"
                     /locus_tag="VBD11_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6954399.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLTPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            5213..6181
                     /locus_tag="VBD11_001453"
     CDS             5213..6181
                     /locus_tag="VBD11_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6954400.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(6221..7675)
                     /locus_tag="VBD11_001454"
     CDS             complement(6221..7675)
                     /locus_tag="VBD11_001454"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6954401.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         2532 a         1449 c         1443 g         2420 t
ORIGIN      
        1 cgttgaatat ttaaagtggt actatctttt aaattcaaac tgtttaaatc tgtctgatct
       61 ttagttgtca atacagatga tgtaatcact tgttcagcac cttcattcaa aatataggtt
      121 tgagctgggt tattttcaat agcagcacta acagctctac ctaaaccgtt tgctaaacct
      181 gataaaaaaa ctaccatgaa catcataaca atgattagca attcaatcag gaggtatttt
      241 ttcttataaa aggttaattc tttccaagca tgtgacatgg tttttctcct ttgatctttt
      301 gatgtgaaca tagtatcaca gctttatgac ttgaatatga cctctaaaaa taatgatatt
      361 ttatcattaa attatttaaa aaatagcttg acaatttttt agtaagcgat tacaattcta
      421 attatgaaac cgttcccaca ataaaagaaa ggagtttcta tgagtacgat tagacaagtc
      481 gccgagaaag ctggcgtatc aacatctact gtctcaaggt atatatccca aaatggttac
      541 gttagtcaaa aagcaagtca aaaaatcgag caagctatcc gcgaactcca ttatgttcca
      601 aatttccttg cacaatctct gaaaaccaaa aaaaatcaac ttgttggatt actacttccc
      661 gatatttcaa accctttctt tccaagactt gctagaggag ttgaagaatt tctaaaagaa
      721 cagggttatc gtgtcatgct tggcaacaca aataacaaga gccatttaga agaagagtac
      781 ttaaatgtgc tattacaaag taatgctgca gggattatta caacacacga tttcaccaaa
      841 aatcatcctg aaatagatat tccagttgta gttgttgacc gcgttaatca agaaacacag
      901 tacggcgttt tctctgataa taaagaggga ggaaaattag cagctcaagc tatttggaca
      961 gcaggtgcta ccaatatatt actcattcgt ggtccgctag ataaagctga taaccttaat
     1021 caaaggtttc aaggaagcca aaattaccta ttaaacaaag gggctcgttt tgctatcgaa
     1081 gatagtgcca gttttgattt tgcagagatt caaattgaag cgaaaacttt acttgaccac
     1141 catccagata ttgatagcat tatcgctcca tctgatatac atgctattgc ctacctacac
     1201 gagattctta atcgagggaa gcgaatccct gaagatgtac aaattattgg ttatgatgat
     1261 attttaatga gccaatttat ctacccttcg ttgtcaacta ttcaccaatc atcatatatt
     1321 atgggacaaa aagcagctga attaattttc aaaattacta atcaattacc tattaccaat
     1381 aagcgtatta aattacccgt tcattatgtc gaacgtgaaa ccctaaggag gaaaatagat
     1441 gagtaacatt gtcattatcg gtagtatttc catggactta gttatggaaa ccaatcgtat
     1501 cgccaaagag ggagaaaccg tatttggcca acgtttttca atggttccag gaggtaaagg
     1561 tgctaatcag gctgtcgcta ttgggcgtct tagccaagat cgagataata ttactattct
     1621 tggtgccatt ggcgaggatt cttttggtcc cattcttttg gataatttaa acaaaaacca
     1681 tgttactacg gattttgtgg gaacgatacc atcttcttca ggtgttgccc aaatcactct
     1741 ttataataat gataatcgaa tcatttactg tcctggagcc aacggaaaag ttgatactaa
     1801 aaagtggtct caggagtggt caataattaa agaagctgat ttagttgtat tacaaaatga
     1861 aattcctcat caagcaaata tgaaaattgc taatttttgt aaagaacata gtattaaatt
     1921 actctataat cctgcaccat ctagaaagac tgatattgag atgctcgata aggtcgatta
     1981 tttcacacca aatgaacacg aatgtcaaga gctgttccca aaccaaaaac tagaggatat
     2041 attagccaca tatcctgaaa aactaattgt aacactcggc acaaaaggag ccatctactc
     2101 tgatggaaaa gaaagccatt taattcctgc cttagaaact aaagctgttg atacaactgg
     2161 tgcaggtgat acttttaacg gtgcattcgg ttatgctatc tcgaaaaaat ttaagattgc
     2221 taatgcctta cgttttgcaa ctctagctgc tcacttatcc gtgcaaaaat ttggtgcaca
     2281 gggtggcatg cctacaatta aagaaatgga ggacaaccaa cattatgaaa aagactggta
     2341 tattaaatag tcatctcgct aaattagcag atgaccttgg gcatactgat caagtttgta
     2401 ttggagatct gggtctacct gttccaaatg gcattccaaa gattgatttg tctttgacct
     2461 ctggaatacc tagtttccaa gaggtacttg atatctattt agagaatatc ttagttgaaa
     2521 aagtcatttt agcagaagaa atcaaggaag caaattcgga tcaattatca aggttattag
     2581 ctaaacttga taactctgtt aacatagagt acgtcagtca caatcattta aaacaaatga
     2641 cccaagatgt caaagcagtt attcgtacag gtgaaaatac accgtattca aatattattc
     2701 tacaatctgg tgttatcatc tagaaaggag tttagtccat gaaaattgac atgaggaata
     2761 tctccaaatc cttcggtact aataaagttc tagagaagat tgatttggaa ttacaatctg
     2821 gtcaaataca cgcacttatg ggtgaaaatg gtgcgggtaa atcaacctta atgaacattc
     2881 taacaggact ttttccagca agtacaggaa ctatttatat tgatggcgag gaacgaactt
     2941 tttcaaaccc tcaagaagca gaggaatttg gtattagttt tattcaccaa gagatgaata
     3001 cttggccgga aatgactgtc ttggaaaatc tctttcttgg acgtgaaatc aagacaactt
     3061 ttggcttact caatcaaaaa ttaatgagac aaaaagcact tgaagctttt aaaagattag
     3121 gtgtgactat tcccctagac atcccgattg gtaacttgtc tgttggacaa caacaaatga
     3181 ttgaaatcgc caaaagtctc ctaaaccaac tctctatttt ggtaatggat gaaccgactg
     3241 ctgctttaac agatcgtgaa actgaaaatc ttttccgagt tatccgaggt ttaaaacaag
     3301 aaggagtggg cgttgtctat atctcccatc gaatggagga aatttttaaa atcactgact
     3361 tcgtaaccgt catgagagat ggtgtcattg tagatacaaa agaaacatca ctcacaaatt
     3421 ctgacgaatt ggttaaaaaa atggttggcc gaaaattgga agattactat cctgaaaaac
     3481 attctgaaat tgggcctgtt gcttttgaag ttagtaacct ctgtggagat aatttcgagg
     3541 acgtttcctt ttatgtccgt aaaggcgaga ttcttggatt ttctggatta atgggagctg
     3601 ggcgtactga agttatgcga actatctttg gaattgataa aaagaagtct ggtaaggtga
     3661 aaattgataa ccaagaaatc actattacta gcccaagcca agctatcaag cagggcattg
     3721 gttttttgac cgaaaataga aaagatgaag gtctcatcct agacttcaat atcaaggaca
     3781 atatgactct ccctagcact aaagatttta gcaaacatgg tttctttgat gaaaaaacaa
     3841 gcacaacatt tgtgcaacaa ttgattaatc gcctctacat taaatcaggg agaccagatt
     3901 tagaggttgg aaatctttct ggaggtaacc aacaaaaggt cgttctcgct aaatggattg
     3961 gtattgctcc taaagtctta attcttgatg agccaacacg tggtgttgat gtcggagcta
     4021 aacgagagat ttaccaatta atgaatgaac tagcagacag aggtgttcca attgtcatgg
     4081 tatcatcaga cttacctgaa attctaggtg ttagtgaccg catcatggta atgcacgaag
     4141 gtcgaatcag tggagaactc agtcgaaaag aagctgacca agaaaaagtt atgcagctag
     4201 ccacaggagg taagtaaagt gaaaaatatg atgaaatata tatcagagtt aacaaccctg
     4261 attgcattag ctggtttaat ggttattatc acactcatta atcctaattt cctcacaacc
     4321 aataatctac tcaatttatt attacaagta actgcaaatg gttttatcgc tttcgggatg
     4381 accttcgtta ttttgactgg cggtattgat ttatcagtcg ggtccatatt agccctatct
     4441 agtgcattaa cagctggatt aatcggccac ggagtacccg ttggtattgc tatcgtttta
     4501 tctgttgcct taggtggcat cttgggaatg ctaaacggac tcttcatttc ttacggaaaa
     4561 ttaacaccat ttatcgttac actggcaaca atgactatct ttagaggagc aacgctagtt
     4621 tattcaaacg gcaacccaat taccgctgga ttatccgata gttttctatt ccaatttttg
     4681 ggacaaggct atattgttgg tatcccattc ccagtcattc tcatgtttct tacttttatt
     4741 attttgtata ttttgttaca taaaacagcc ttcgggaaat ctgtttacgc tttaggaggt
     4801 aatgaaaaag cagcgtatat ctcgggtatt aagttaaaca aagttaaaat tatcatctac
     4861 actatctcag gtatcatggc ttccatttct gggttaatta tcacatcacg ccttagctca
     4921 gcacaaccaa cagctggagc aagttatgag atggatgcta ttgccgccgt tgtcttaggt
     4981 ggaacgtctt tatctggtgg gaaaggacgt atcattggaa cattgattgg tgctctcatt
     5041 attggtgtcc tgaacaatgg attaaacatc attggtgtct ctgcattttg gcaacaagtt
     5101 gtgaaaggta tcgttatttt aatggctgtt ttacttgatc gctttaaagt agcaaaatag
     5161 tctttactct aatttctata agttcattat attttatcta aaggagtttc aaatgaaatt
     5221 tggaaaaaaa cttggctttc tagccttatt gatgtctatt gtcctcattc tcggtgcatg
     5281 tggtaaaact ggacttggaa attcttctgg gaattctaca aaaaatgtaa ccaaaaaatc
     5341 tgctaaagat ttgaaactag gtgtatctat ttcaacaact aacaacccct actttgttgc
     5401 gatgaaagac ggtattgaca aatatgcgag caataaaaaa ataagcatta aggtagctga
     5461 tgctcaagat gacgccgcac gtcaagcaga tgatgttcaa aactttatta gtcaaaacgt
     5521 agatgctatt ttgattaatc ccgttgactc aaaagctatt gtaacagcta ttaagtctgc
     5581 aaacaacgct aatattcctg ttattctaat ggaccgtggt agtgagggag gcaaagtatt
     5641 aactactgtc gcttcagata acgttgctgc tggtaagatg gctgcggatt atgccgttaa
     5701 aaaactaggc aaaaaagcaa aagcctttga attatctgga gtaccaggtg cctctgctac
     5761 tgtagaccgt ggtaaaggat ttcattcagt tgcaaaatct aaacttgaca ttctttcaag
     5821 ccaatctgcc aattttgacc gcgccaaagc tttgaatact acacaaaata tgattcaagg
     5881 acataaagat gtccaaatta ttttcgcaca aaatgatgaa atggcacttg gggctgcaca
     5941 ggctgtcaaa tctgctggct tgcaaaatgt tcttatcgta ggaattgatg gtcagccaga
     6001 tgcccatgat gccattaaaa aaggtgatat ttcagccact attgcacaac aaccagctaa
     6061 gatgggagaa atagctattc aagcagcaat tgatcattat aaaggcaaaa aagtagagaa
     6121 agaaacgatt tctccaattt accttgttac caaagacaat gttgaaaaat acaattggta
     6181 aaagtacaca aaaagtcgtg aaaattcacg actttttata ttataatttt gctaacatat
     6241 tatcgatatg ttgaactgat ttttctttac ctaataaata aatagtatct ggtaactctg
     6301 gtccatgcat ttcaccagaa accgcaatac gaataggcat aaataggttc ttacctttga
     6361 tgcctgtttc tttttgaaca gccttaattt gagggaaaat agtatctcta gtgaattctt
     6421 cgtcagaaag cgatactaac ttttccttaa aagcagataa gactgttggg actgtttctg
     6481 ctgccattac ttctttttcg gcttctgtta attctggaaa atctgcaaag aaaaggtctg
     6541 ttaatggtac aatctcatca gccgatttta attgaggttt ataaagctca actaattttt
     6601 cagctttgtc tgtaagtcga ccagcttctt ctaagaatgg tttgcaaagt gcaaaaacac
     6661 tctcaaaatc agcatttttt aggtaatcat tactcatcca atccatcttc ttctgatcga
     6721 atgctgctgg tgatttgcta agacgatttt catcaaatag attaattaat tgctcgcgtg
     6781 agaagatttc ttcttctcca ccaggattcc aaccaagaag ggcaatgaaa ttaaagacag
     6841 cttccgacat ataacctttt ttacgatagt cttcgataaa ttgaagagta tttgtgtcgc
     6901 gttttgataa tttcttacca gtctctgagt ttataatcaa tgtcatgtga ccaaattgtg
     6961 gcgcttccca accgagtgct tcataaacca ttaattgttt tggcgtattt gcaatatggt
     7021 catcgccacg aataacatgc gaaatttgca tgtcatgatc atcaatgact actgcgaaat
     7081 tgtaagttgg ataaccatct ttcttctgaa taacccagtc tccgccgata ttagagcctt
     7141 caaactcaat gtcacctttt accatatcag tccacttata aatcccagat tcattaacag
     7201 caaggcgtac tgtcggaatg atgcctgctg cttcacgttc tgcaatgtaa gcttcttttt
     7261 cagtttctga cattccgata aattcgttga tatagcgagg tgtttcgcct gccaattctt
     7321 ggcgttcacg ctctgctgct aattcttcct cagtcacata ggacttgtag gctttacctt
     7381 ctgctaacaa ctgatcaata taacgttggt agagctcaag gcgttctgat tgacgataat
     7441 tttcatgtgt ttcagggctt tcatcccagt ccatacctag ccaacgtaag ttctccaatt
     7501 gagagcgttc accatcttcg acgtgacgtt ttcggtcagt atcttcaata cgaataacaa
     7561 aatccccacc atgatggcga gcataaaggt agttaaagag cgcagtacgc gcatttccga
     7621 tgtgtaacaa tcctgttgga cttggtgcat aacgcacacg aatcttatta gccaaaattc
     7681 tactcctata ttttctgata gtaaactttt tcagtttaac tgaattagtc aagggggcaa
     7741 aacatcattc aaactcttac agatgattgc caatcaaccc tattatacca ctaaatcact
     7801 aaaaatgata atcagtaata atagtcacac taattttcga taaa
//
LOCUS       DATROY010000085         7797 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.086, whole genome shotgun sequence.
ACCESSION   DATROY010000085 DATROY010000000
VERSION     DATROY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            155..628
                     /locus_tag="VBD11_001455"
     CDS             155..628
                     /locus_tag="VBD11_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6954402.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            650..1291
                     /locus_tag="VBD11_001456"
     CDS             650..1291
                     /locus_tag="VBD11_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6954403.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(1327..2229)
                     /locus_tag="VBD11_001457"
     CDS             complement(1327..2229)
                     /locus_tag="VBD11_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6954404.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMASERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            2404..3894
                     /gene="lysS"
                     /locus_tag="VBD11_001458"
     CDS             2404..3894
                     /gene="lysS"
                     /locus_tag="VBD11_001458"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6954405.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3969..4439)
                     /locus_tag="VBD11_001459"
     CDS             complement(3969..4439)
                     /locus_tag="VBD11_001459"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6954406.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(4454..5647)
                     /locus_tag="VBD11_001460"
     CDS             complement(4454..5647)
                     /locus_tag="VBD11_001460"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6954407.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(5665..6315)
                     /locus_tag="VBD11_001461"
     CDS             complement(5665..6315)
                     /locus_tag="VBD11_001461"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6954408.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(6296..7405)
                     /gene="ribD"
                     /locus_tag="VBD11_001462"
     CDS             complement(6296..7405)
                     /gene="ribD"
                     /locus_tag="VBD11_001462"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6954409.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(7599..7713)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         2507 a         1594 c         1200 g         2496 t
ORIGIN      
        1 taatttaaca ttccaaatat actgttgcga tatttattat cattattccc tgaaatggta
       61 ttcaagctta tttctaagac tcagtagaaa tttactataa tcatctttaa atgttataat
      121 aaatatgaaa caaacttatt agttaagggt cattatgagt aaaaagaata aaatcaaaaa
      181 aactttagtt gatcaaattt tagataaagc aaaaatcgaa catgatagtc tacaattgga
      241 tgctttacag ggagatttac ccaacggtat tcaaaaacaa gatatcttta agacattagc
      301 gctaatcggt gataaaactg gtcccataat aggtatctta cctctcaccg agcatttatc
      361 cgaaaaaaag ttagctaaaa tttcaggaaa caaaaaggta caaatgatac ctcaaaaaga
      421 tctccagaaa ataacaggtt atattcacgg agcaaataat ccaattggaa ttcgccaaaa
      481 acataattac cctattttta tcgatactat cgctttagaa aaacaagaac tcattgtctc
      541 tgcaggagaa attggacgtt ctattcgtat taatagtgag gttcttgcag atttcgtaaa
      601 tgctaaattt gccgatataa aggaataata attaggattg gagaaaaata tgaaatttta
      661 tcttgttaga cacggaaaaa cacaatggaa tttagaaggg cgtttccaag gtgctaacgg
      721 agactcacct ctccttgaag aagctattga agaattagaa gaacttggac aatacctatc
      781 atctattcat tttgatgctg tttattctag cgacttaggt cgtgctagag atactgttaa
      841 tatcctcaat gatgctaatt catgcccaaa agaaatccac tatacttctc agcttcgtga
      901 atgggcattg ggaactttag agggttgtaa aattgcaact atgcaagcta tctatcctcg
      961 tcaaatgacc gcattttatc aaaatccctt acaatttaaa catgacatgt tcggtgcaga
     1021 atctctttat cagacaacgc atcgtgtaga gagtttctta cgttctttag cttcaaaaaa
     1081 ttacgacaag gtccttattg taggacatgg agcaaatcta actgcgtcta ttcgttcctt
     1141 actgggttat caatatggta gtttacacta caaagataaa cttgataatg ccagcctcac
     1201 aatcatagaa actcatgatt ttaaagactt caactgtctg acttggaacg ataaatctta
     1261 tttaaggcaa gaggtcaaaa tgacacacta aaataatcca ctagattcat ctagtggatt
     1321 atttaattat ctagcatact tcatgcgtaa tactttttcc tgttttgcag ttaggcgcag
     1381 taaaataaca actttgtcaa cgctcatatt actctcaagc agacgctctg atgccatcat
     1441 taaaccatta ttaagtccca tttcaaggac tggattactt ttatcgttga tatcaaaaat
     1501 aaatttatta tcaggtaagt caaacaacac tttaacagca ccgaataatt gctcaaagtt
     1561 atcaatagca acatcataaa ctggtttaat tcccggtttt aaagaacgtc ctctatggtt
     1621 gaaccacata gaatgccaac caccattaaa tgcacccata atatcattat catatgaatc
     1681 acccacataa agtgtagttg aagggttcat atcaaattgc tctgctgcta aattaaaaat
     1741 ctccttttca ggcttttgaa aaccagttgc ttgactaact ataacacgtt ttggatcaac
     1801 atagtcataa agtcccaatt ttttaacttt tttcaattga tgttccgtag ggccatttgt
     1861 aataattccc attggtacat tttttgattt caaaaagtca agtgtcatac gcatctcatc
     1921 aagcattgta atattttcaa gttcatgttc gtatatttct tgaaaataaa taccagtagc
     1981 ttcatcaatt tcacgataac caaactctaa aagcgtttct ttgcatcgcc agaaacgaaa
     2041 atattctgtt gtccattcac cagccattac tcgtggaaag ccaatatctg aataatgacg
     2101 aaagcgaata taagcttggt taatagcgct catatcaaaa tcaggaaagc atttctccac
     2161 agcaatacgg taaggtgctt gttgatcgta aattgtatca tcaacatcaa aaacaattga
     2221 agtaatcatg attatccttt tctttctgaa ttttcaattg ttaattatac ctttttctac
     2281 ccttattttc aaatggtgaa aagtctatta aaaatgagaa taacaccata aactcataaa
     2341 ataaattatg aattatcagt gtttttgtgg tacaatagga tgaatactaa cttggaggaa
     2401 attatgtcta accaacatat tgaagaatta aatgaccaac agattgttcg tcgtgaaaaa
     2461 atggctgctt tgactgaaca aggcattgat ccttttggta aacgatttga acgtacagca
     2521 acatctggtc aattaaatga aaaatacgct gacaaatcaa aagaagactt gcacgacatt
     2581 gaagaaactg ctacaatcgc tggtcgttta atgactaagc gcggtaaggg gaaagttggc
     2641 tttgcgcata tccaagatcg cgaaggtcaa attcaaattt atgtccgtaa ggattctgtt
     2701 ggcgaagaaa actacgaaat ctttaaaaaa gctgatttag gtgacttcct tggtgttgaa
     2761 gggcaagtaa tgcgtacaga tatgggagaa ctttctatca aggctactca tattactcac
     2821 ctttcaaaag cactccgtcc acttcctgaa aaattccacg gcctaactga tattgaaaca
     2881 atctatcgta aacgtcatct tgatttaatc tcaaatcgtg acagctttga ccgttttgta
     2941 acacgctcaa aaatcatttc agaaattcgt cgctttatgg atagcaatgg tttcttagag
     3001 gttgaaacac ctgttcttca taatgaagct ggtggtgctt ctgctcgccc atttatcaca
     3061 catcacaatg cacaagacat tgacatggtt cttcgtatcg caactgaatt gcatctaaaa
     3121 cgtcttattg taggtggtat ggagcgtgtt tatgaaatcg gacgtatttt ccgtaatgaa
     3181 gggatggatg caacccacaa tccagagttc acatctattg aagcctacca agcctatgct
     3241 gactaccagg atattatgga cttgacagaa ggtattatcc agcatgttac aaaaactgta
     3301 aaaggtgatg gaccaatcaa ttatcaaggc acagaaatta aaattaatga accattcaaa
     3361 cgtgttcata tggttgatgc ggttaaagaa atcactgata ttgacttctg gaaagaaatg
     3421 actcttgaag aagcacaagc tttggcacaa gaaaaaaatg ttcctcttga aaaacatttc
     3481 acaacagttg gtcacatcat taacgccttc ttcgaagaat tcgttgaaga cactttaatt
     3541 caaccaactt ttgtctttgg tcatccagtc gaagtgtctc cacttgctaa gaaaaatgat
     3601 acagaccctc gttttacaga ccgtttcgaa ctctttatca tgactaaaga atatgctaat
     3661 gcttttacag aattaaatga cccaattgat cagctatctc gctttgaagc acaagcatct
     3721 gctaaagaac ttggcgatga tgaagcaact ggagttgatt acgattatgt tgaagcactt
     3781 gaatatggta tgccacctac tggtggacta ggtattggta tcgaccgtct ctgtatgctg
     3841 ttaactgaca caacaactat ccgagacgtc ctacttttcc ctacaatgaa ataagaatat
     3901 attaaaaccc acccttattt aaggtgggtt ttaatattac ctctactgaa ctttgttagg
     3961 tttctgattt atcgctcacg caatttttta tcaaggttta ccatttcaat agccgaaaga
     4021 gcacaatcat aacctttatt tcctgctttt gttcctgccc tctcaatagc ttgttcgata
     4081 ttatccgttg ttagaacacc aaagatatga gggatatctg actggctatt taaatgacca
     4141 ataccttttg tcacctcatt acaaacataa tcataatgat cagtgctgcc tttaatgact
     4201 accccaagac agataatagc atcataatct ttatataatg ccatttttct agccatatag
     4261 gggatttcaa aagcaccggg tacccataca atatctatat cttcttctga aacaccatgt
     4321 ctcaacagtc catctactgc tcctgataat aatttagatg taatcaattc attgaaacgt
     4381 gagactacga ttcctatttt catttcgttt gccactaatt gtccttcaat aattgtcatt
     4441 ttttcttcct cctttaatct aaaagatgtc ccattttttc ttttttagtt tttaaataat
     4501 taaggtcata agctgttgct gccacctgta atggcactct gtttttgacg caaattcctt
     4561 ctgcttcaag ttgttggatt ttatctggat tattggttag taagtttata gaatttatac
     4621 ctaaatcttt taaaagttga gcactaactc catagtctct ctcatctcct tcaaaaccta
     4681 aagccaaatt agcttctaaa gtatctaagc cttcttcttg caaatgataa gcttttaact
     4741 tattaatcaa accaattcct cgaccttcct gtctcatata aagaaggacg ccacgacctt
     4801 cagcctctat ttgttggagt gcattagcca gttgttcccc acaatcacat ctcaaggaat
     4861 gaaagacatc acctgtcaaa cattctgagt gtaaacgaca taaaacatct tcaccactac
     4921 taatatcccc tttaacaagg gcaacatgtt ccttatttgc cacaacttca ctatatccat
     4981 aagccttaaa atggccgaat tgcgttggta gctgaatctc aatttctcta accacccctc
     5041 cctcttcttg acgtctatag tcttgcaatt gttttatggt agcgatagct aaatcgtgct
     5101 tttgagcaaa tgctaataac tcatcctttc tcatcatact accatcttca gccattattt
     5161 cacaacaaag tccacattcc tttaatccag ctaaacgaca tagatcaacg gttgcttcag
     5221 tatgtccatt tctagctaaa acacctcctt ccttagctaa caagggaaat aagtgcccag
     5281 gacgtctaaa gtctttagct ttgctactgt cgttagcaac catcttagct gtcaaagctc
     5341 tatcttccgc agaaatccca gtactagttg atagatgatc aatggatatg gtaaaagctg
     5401 tttcatgatt atcagtatta tggctagcca tttgagctaa ttctaacttt tcagcatagt
     5461 aattagacag aggtaagcaa attaatcctt tcccaaattt agccataaaa ttaatcgact
     5521 cttttgtcac catttcagcc gcacaaataa ggtccccttc attctctcta ttttcgtcat
     5581 ccatcaaaac taccatttcc cctgatttga tgtcttgtaa aagttttttt attggactaa
     5641 aggccattcg ctttctcctc tctcttagta gccattttct gataaaaaag accaatccat
     5701 tactgatgaa gtgtgattag ttctattagt cggtgctaaa aatttctcag catatttgcc
     5761 taaaacatcc acttcgatat ttaataaatc cccaaccttt tttaaggata aattagtttg
     5821 agccatcgtg tggggaacga ccgaaacttc aaaagaatgc tctgtaaatg ataccaccgt
     5881 taggctaatc ccatctagag ccaccgaacc tttctctata atataagaca taatactggg
     5941 cggagcatca attgaaaatc tgacagcaat atcatccttt tggatttttt ctatttttcc
     6001 aaccccatcc acatgtccaa gaacaaaatg cccccctaac cttgagtcac ttctgagtgc
     6061 taattctaag ttaactttag aatgaagtcg taaagcagct aagcttgttc gcatcatact
     6121 ttctggcatg acgtctgcta taaaagcttt ttcacttttg tcaaggactg tcaaacaaac
     6181 accattaaca gctacgctat ccccttttct taataaaggg actagtttgg gaacatcaat
     6241 acttaattgt tgggatttta tgccattccg aatacgactg acttgaccca tttcctcaat
     6301 aattcctgta aacataatgt tctacctttc cttcaataac aacgtcagaa tccatacgac
     6361 taagttcaat atcttttact ctgaaagctt ggttaagcca atccaagcct tctccagaaa
     6421 tagctgtttt tgctttcaga cctcctatta ttttgggtgc aataaaaaca attagtctgt
     6481 taacaatacc agcttctaaa gctgaaaaat gtaaactact tcctccttca ataagaaggc
     6541 tatcaatccc ttcttgtcct aaaatagtca ttaatacttt caaatccagc tttccatctt
     6601 ttcttgggac cttaatcaaa cggcaaccca tttcttttaa tgtttgttgc tgagcaagat
     6661 tatctgaaca tgttgcaatc catgttggta ttgttttagc gctcttaact aggtaagagt
     6721 ctaaaggtat cttcaactga ctatcacata cgatcctcac aagcgcttca cctttaggaa
     6781 tacggcaagt taaacgtgga ttatcagcta atacagtatt gataccgacc ataatagcac
     6841 tacatttttg cctcaatttc tgaacagatt gtcttgagtg ctcattggat atccacttag
     6901 aatcgcccgt ctttgtagct atcttcccat ctaaagtcat agcatatttc aaataaacaa
     6961 aaggctgctt ataggtcatg tgaaaaataa atctttcatt cagagcatcg cattcttctc
     7021 ttaggatacc aacttcaaca tttaaacctt cttttcttaa caaggcaatc ccttttcctg
     7081 aaactaaagg atttggatct aaacttccaa ctactacttt tttgatacca gatttaataa
     7141 gagcttcagt acaaggtggt tgcttaccca catgacagca tggttctaaa gtcacgtaca
     7201 gagtacttcc agagatatcc tcatcagcat ttttaatagc ctgtctttcc gcatgtaaat
     7261 ctccaaatcg tttatgataa cctttggaaa ttatacgatc atctttaaca ataacagcac
     7321 ctactaatgg gttgggagca acgaacccca tacctttctc agcctctttc aaggctaatg
     7381 ccatgtagtc ttcttttttc atcatttcct ttttccttct aaaataaaaa cccttagctt
     7441 ctaaaataga aaactaaggg gagggatgag aagtcgttga ttatactaat tagcattata
     7501 ttttaggtag cacaaaaaaa ctaaagactc cctatagaaa actataggga gtctctcatt
     7561 aatccagcac aaataaactg caagtagcag acaatcactg aattcttctc tcatccagac
     7621 tttactgtcg gtactggaat cacaccagtt catgctcgaa agctcgcgga ctataccgcc
     7681 ggttaggatt ttcacctgac cccgaagaaa ttctatttga cagttatttt actaaactca
     7741 tttttatttg tcaataaaaa taatgtgttt tttattataa aataataatt aagtagc
//
LOCUS       DATROY010000086         7680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.087, whole genome shotgun sequence.
ACCESSION   DATROY010000086 DATROY010000000
VERSION     DATROY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            70..1794
                     /locus_tag="VBD11_001463"
     CDS             70..1794
                     /locus_tag="VBD11_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEO6954410.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPD
                     TLKVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNITNSEVDKLASDYS
                     FRINTSNDVNDVKRLLNEFYNAVARKQLDTNSADYRSKIDNISTTGLAIALEAKEIYE
                     ANKSILPHRYKDSVGTYVNSFEERRSPGKFNIWNGQEGFNAAQKLLEDVKKLLLELQN
                     LTKNNKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVKDKYVDILSV
                     QKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPVAKGTYDVKYVD
                     TEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFTAGAQVRTYVYEKVA
                     KPVAKGTYDVKYVDTEGKEVAKSRHFEGEEGAAFVTSAKEVAGYKLVRTEGAVSNVFT
                     AGAQVRTYVYEKVKPEVKPDVKPEAKPEAKPEVKPDVKPEAKPEAKPEVKSDVKPEAK
                     PEAKPATKKSVNTSGNLVAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLIT
                     IVLKHKKN"
     gene            2000..2539
                     /gene="nusG"
                     /locus_tag="VBD11_001464"
     CDS             2000..2539
                     /gene="nusG"
                     /locus_tag="VBD11_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6954411.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2586..3791)
                     /locus_tag="VBD11_001465"
     CDS             complement(2586..3791)
                     /locus_tag="VBD11_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6954412.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
     gene            complement(3856..5052)
                     /locus_tag="VBD11_001466"
     CDS             complement(3856..5052)
                     /locus_tag="VBD11_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO6954413.1"
                     /translation="MTYQKTVVLAGDYSYIRQIETTLKSLCVYHENLSIFIFNQDIPQ
                     EWFLAMKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQYISADTV
                     LYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWRQENMTI
                     KLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHKFIFDIP
                     ITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHPIKTQNY
                     RLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLHKTYTPP
                     IIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPILTTRECNSQLVPETVFASIDDLI
                     NYLQSI"
     gene            5257..6102
                     /locus_tag="VBD11_001467"
     CDS             5257..6102
                     /locus_tag="VBD11_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO6954414.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(6145..7392)
                     /locus_tag="VBD11_001468"
     CDS             complement(6145..7392)
                     /locus_tag="VBD11_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954415.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT         2688 a         1188 c         1442 g         2362 t
ORIGIN      
        1 taaattaaaa taaacgtggt cctatcctaa taaattagga tttttaacta actatataag
       61 ggagcgaata tgaataataa cgaaaaaaaa gtaaaatact ttttaagaaa aacagcttat
      121 ggtttggctt caatgtcagc ggcgtttata gtatgtagtg gtattgtaaa tactcctaca
      181 gtgtctgctg atagtcctga tacattaaaa gtcgaaaaat taggcaaatt gaaagatgtg
      241 aaatcagttc atgaactcac acccatatca ataccgaacg aattaaaagg tgctaaagag
      301 caagcacttt cttcaataat ttcacatcct aatataacta attcggaagt agacaaacta
      361 gctagtgact atagttttag aattaataca tctaatgatg tgaacgacgt taaacgtcta
      421 ttaaatgaat tttataacgc agttgcaagg aaacagttag atacaaattc tgctgactac
      481 cgtagtaaaa ttgataatat cagtactaca ggtcttgcga tagctcttga ggctaaagaa
      541 atttatgaag caaataaatc tatattacct catcgttaca aagattctgt tggaacttat
      601 gtgaacagtt ttgaggaaag acgaagtcca ggaaaattta atatttggaa tggtcaggaa
      661 ggatttaatg cagctcaaaa attgttagaa gatgttaaaa aattattact tgagctacaa
      721 aatttaacaa aaaataacaa accaaatatt caagtaccta aacaagcacc tacagaagct
      781 gcaaaaccag ctttgtcacc agaagccttg acaagattga ctacatggta taatcaagct
      841 aaagatctgc ttaaagatga tcaagtaaag gacaaatacg tagatatact ttcagttcaa
      901 aaagctgttg accaagctta tgatcatgtg gaagagggaa aatttattac cactgatcaa
      961 gcaaatcaat tagctaacaa gctacgtgat gctttacaaa gtttagaatt aaaagataaa
     1021 aaagtagcca aaccagtagc taaaggtaca tacgatgtta agtatgtaga cacagaagga
     1081 aaagaagtag ctaagtcacg tcacttcgaa ggagaagaag gcgcagcttt tgtcacttca
     1141 gcgaaagaag tagcgggtta caaacttgtt agaacggaag gcgctgtttc aaatgtcttc
     1201 acagcaggag cacaagtacg tacatatgtt tacgaaaaag tagccaaacc agtagctaaa
     1261 ggtacatacg atgttaagta tgtagacaca gaaggaaaag aagtagctaa gtcgcgtcac
     1321 ttcgaaggag aagaaggcgc agcttttgtc acttcagcga aagaagtagc gggttacaaa
     1381 cttgttagaa cggaaggtgc tgtttcaaat gtcttcacag caggagcaca agtacgtaca
     1441 tatgtttacg aaaaagttaa accagaagtt aaaccagacg ttaagccaga ggccaaacca
     1501 gaggctaagc cagaagttaa accagacgtt aagccagagg ccaaaccaga ggctaagcca
     1561 gaagttaaat cagacgttaa gccagaggct aaaccagaag ccaagccagc aaccaaaaaa
     1621 tcggttaata ctagcggaaa cttggtggct aaaaaagcta ttgaaaacaa aaagtatagt
     1681 aaaaaattac catcaacggg tgaagccgca agtccactct tagcaattgt atcactaatt
     1741 gttatgttaa gtgcaggtct tattacgata gttttaaagc ataaaaaaaa ttaaatttat
     1801 ttttaaacat aaatcgaaag gtctatcctt tcaaaatctt aaggcaaacc tcatcagaac
     1861 tttgcctttt ttatttgaat tttagaatga tttagtcggt actttaattg acagcttatt
     1921 ttattttctt tataatatat attgaaaata gcagtccaat aagccgtaaa aggctttttt
     1981 attaaggaaa ggaaaactta tgttagattc ttttgataag ggttggtttg tactccaaac
     2041 ctactcaggt tatgaaaata aagtaaagga aaatctcttg caacgtgcgc aaacttataa
     2101 tatgttagac aacattttgc gtgtggaaat ccctactcaa actgtaaatg ttgagaaaaa
     2161 tgggaaaaca aaagaaattg aagaaaatcg cttcccaggt tatgtacttg tggaaatggt
     2221 aatgactgat gaagcctggt ttgttgttcg taatactcca aacgttactg gatttgttgg
     2281 atcacacggc aatcgctcta aaccaacacc gttgttagaa gaagagattc gttcaatact
     2341 catctcaatg ggacaaaccg ttgatgtctt tgatactaat atcaaggaag gtgatgttgt
     2401 tcaaatcatt gatggtgctt tcatcggaca agaaggacgt gtagttgaga ttgagaataa
     2461 caaagttaaa ttaatgatca acatgttcgg ctcagaaaca caagcagaac ttgagttgta
     2521 ccaagttgca gaattataaa acttctgaaa aatccatctt aaaataaaga tggatttttg
     2581 ttaatttata attgatttag gtttaattcg ttaattaaaa caaccatatt atcaatagaa
     2641 ttatgattaa cgatatgttt ggttatattc tcttgatgag acgtgttgtt gaaagaataa
     2701 attggctttt gtttcaaatg aactctttgt aaaatattac ctacttctgg accatgatta
     2761 atatcaaggt atgcagtggc taatgttatt aatttttcaa ttcttgcctc aattaattca
     2821 gggtaaacta ggacatttgg atattgttct agtttgctaa gtttatttga cactgttgtg
     2881 taggcaccaa tatggaattg aacagtagga catctacaaa ttagcgattc aatgtgttcc
     2941 aaattatcgg aagatgtaag ggtgaaaaga tgaatatctg aaacagaaag caattccatg
     3001 gacttttcgt aagaaatcac ttttctttgc ttgccggtta attggcaaaa atcaagttgg
     3061 aagtaatacc accatagttc tctaaacctt gcaacgctat tttcattcca aggtttgtca
     3121 ctagttaaaa aatgtataat attaggagtt ctattttcta tgtttagata taaataagat
     3181 cgatgattat agcggctaac gacatcataa gtttgaaagt tccatgtttt gttcagagat
     3241 aaccaacgat tatggcaaac aatgttcaag atactttgat cccctgtttt gacaactcgg
     3301 tggagttctt cagctgtttt tagtaatgtt ttagtgaggc catcttcccg ccatagttct
     3361 agattagcta aaagcatgcc agcattaaac cctccttctt catttttatg gggatctttg
     3421 acggctgcta agtaatgacc tttaaaatct agttcaaata agtaatcaag attagtgtta
     3481 acgataatat ctgaatctaa ataaagtaca cgtgaactag gtatataatc tgctaaaaat
     3541 aatctaaacc atgtactaac agttagaaaa gtcgttagat gagcaatatc atagttatat
     3601 attttaacat taatcagttt tgatcctaga ggctctagtt tcctgtttat attacgtacc
     3661 cattcaactg gggtatcttg attgaataaa taaaaagtta tatttctatt ataaacacaa
     3721 atagacttta ttgttactaa ggctttatcc aagtagtttg aatcaactgc aagtgcaact
     3781 gctttttcca tttaaattct cctagttaag tgtttagaat aatgttgggt atatagtgaa
     3841 tggatgccaa tttttttata tagactgaag ataattaatt aaatcatcga tagatgcaaa
     3901 aactgtttct ggaacaagct ggctattgca ttctcttgta gtaagaattg gtttttcaga
     3961 ttgtgacatt ttctgtagaa tgccctccat ttttccaaaa acattaatat caagataaat
     4021 atcagcttct gctaataatt tatcaataat aggtggagtg taagttttat gaaggcggat
     4081 attttcaaaa gctaataggc ttataagaat gtcagacata actgttggtg ctgcaatgtg
     4141 aaatagacaa tctggaaggg cagtgactaa ttctgagata gatggtatac aatcagaagt
     4201 tgtaacaata aggaaattga gacgataatt ttgtgtttta attgggtggt ctaaaccaaa
     4261 tactttttta ctagatatga tacttgacca ttcaagtaaa ttatagtgcc accatacctc
     4321 acggagtctc atatttgata atgtttgcca aggtttgatt cccgaaatgt agtgaataat
     4381 ttttgggagt ggggtaattg ggatgtcaaa aataaattta tgcccatcga tagcagctcc
     4441 catatcaaaa ccaatttgaa aattgtaggt atcatctaaa taaattacct gattttcaaa
     4501 catgcgatta agaattgttt gatccccttc gttggcattc tcaatttcct tttgattttt
     4561 ttcaattaat ttaatagtca tattttcttg tcgccaacgt tggttgttaa ttaccattac
     4621 tccagcatta aacccatatc caagcccaaa aagagctgga actgcagcta aatatgcatt
     4681 gtctagtgaa atttgaaaga ggttatctaa attagtagta acaactaagt cagagtcaag
     4741 atataaaact gtatcagctg agatgtactg aggtatgaaa taacgagcat aggtcatata
     4801 attaatatga ttaagctttt tattttccca ttttggggat aagtgatcat ggaagagctt
     4861 tacatcctga atttgatttc cagtttgtcc aaccctatct ttcatagcta aaaaccattc
     4921 ttgaggaata tcttgattaa aaataaaaat tgagagattc tcatgataga cacagagaga
     4981 ttttaatgtg gtttcaattt gtctaatgta ggaataatca ccagccaaaa caactgtttt
     5041 ttgataagtc ataaaaaatc ctttatttat attagttaga tatagtaaat ataaatttaa
     5101 aaactattag tattattcta tcataggtga gtgtgattca gaaattatta ttaaattaat
     5161 tatggagttt atatagtaca acatattctt tttcagaaaa aacagctata ttttgctata
     5221 ctttaatcaa atatcaatat tagaaagtga ttgcctatgt tgtctgttgg tttagtttta
     5281 gagggtggcg gaatgagagg tctttatact gctggagttt tagatgcttt tctagatgca
     5341 ggaataaaaa tagatggtat cgtatctgtc tctgcgggtg cattgtttgg tgttaatttt
     5401 gtatctagac aacgagagag ggctttgcga tacaataaaa agtatttatc ccaccctgaa
     5461 tatatgagtc taagatcatg gcttcgaaca gggaattttg ttaataaaga tttcacctat
     5521 tatgaagttc ctatgaaatt ggatgtattt gacgatgaag catttaaaaa atcaagtatt
     5581 gatttttacg tagttgctac agagatgaca tctggtaaac ctgaatattt taaaattgat
     5641 agtgtttttg aacaaatgga aattttacgt gctagttcag cattaccagt agtctcaaag
     5701 atggttgatt ggcaggggaa aaagtactta gatggtggtt tatctgatag tattcccgtt
     5761 gattttgccc gtggtttagg atttgacaag ttgattgttg tgatgactag gccgctcaat
     5821 tatcagaaaa agccttcaag tggacgattg tataaaactc tgtataggaa atatcctaat
     5881 tttgtaaaga cagcctcgaa tcggtaccaa cagtataata atagtcttga aaaggtcatg
     5941 agccttgaaa aaacaggcga tctatttgca attagaccga gtaagagctt ggttattggc
     6001 cgcttagaga agaatccgga taaacttgat agtatttatc agcttggtat gaaatatgct
     6061 aaaagtgtga tgcctgagct gaatagttat ctaatgaaat aaagaaaaca gtatttctca
     6121 attgaggaat actgttttgt aatatcagtc agttggtttg acatctaatt gtgttaaatt
     6181 agtgtgatat ttttgacgtt ttaattgctt aatgcttagc attgataaga aactaacgat
     6241 atagagactt gctaagaaaa gcatgaccat agagacattt gaaacgtcta gaatcattcc
     6301 gatgataaca gacgaaaagc cacctattgc acgaccaaca tttaagataa gattgttagc
     6361 tgttgaacga atatgatgtg gatagagacg tgtaatcatg gcaccatagc ctgcaaacat
     6421 accattaaca aagaagccga caactgcccc gcctataatc atagaaggca ttgttgttgc
     6481 aaattgaaag agatagacac aaattgcaga tgatagaagg aaacacccgt atactaaacg
     6541 cggtccaaat ttatctaaca attgaccaaa tactagcatg ccaagacaca ttccaaaaat
     6601 ggttgcaatc atccataatg atgaattctt gacagaaacg tttaagttgg tttggataat
     6661 agttggtaac cagttcatca tgccaaagta tccggcaatc tgaacagttg tcataaccat
     6721 tagtgctagg ctctgtgtgg ctaatacagg agtatcaaat aaagctttta tactgatatc
     6781 tagaggttca tcatctgcgt ctgtattata ttgatctaag atatctttat catcaatagc
     6841 aaattgcatc cataggacga gaactatagg aagaaggcca aagagaaaaa gtccccgcca
     6901 accgagtgct ggtgctagcc atcctgctaa gagtgctgag ctaatagatc cgacttgacc
     6961 agcgatacca tttagtgaag aaatccggcc cattttatta gctgggacaa ttcctgccat
     7021 aatggcaata gcgataccat attcgccacc aacgccaatt cctgcaatga agcgcatgat
     7081 atagagatat gataaattag tagtgaagaa aactaaacca gttgcgattg aaaagattag
     7141 aatcgtccat ttgaaaactt tgaatttatt gtagcgatca gctaataaac caaagattag
     7201 tcctcccacg agcattccca agttggtaat tgtcccaatc caaccagcct gtgccgcaga
     7261 taaatgaaga tcagtaatga tagttgacat agaaaatgca aggaacatga tatttaaatc
     7321 atcggttccg gatgcgacaa tggcagcgat tagggcgcgc caattattct tattgatatt
     7381 taatggtgac ataagtctcc tcctttaatg atggctcaca gaccaccgtt attaaatact
     7441 gtcaatagaa aaaattctaa agcagagatg tcatagctgc ttcaactcat tataatgtaa
     7501 aagaaaaaac tcaagctgct acccttgagt tctataaacg ctcgcaaaaa tagtaggatt
     7561 tctgtataga atcctatttt ggtcgcttat atgtctcaca ggacacggtt aaagcattat
     7621 gtcatttgga aatagtatag agtaagttta gtttattgtc aaggataagt ggggagagaa
//
LOCUS       DATROY010000087         7651 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.088, whole genome shotgun sequence.
ACCESSION   DATROY010000087 DATROY010000000
VERSION     DATROY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..135
                     /locus_tag="VBD11_001469"
     CDS             <1..135
                     /locus_tag="VBD11_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF910 family protein"
                     /protein_id="HEO6954416.1"
                     /translation="DIEVMKIELQRLYDNGLISRDDYLKAELILRREHRLELEKENKK
                     "
     gene            132..1100
                     /locus_tag="VBD11_001470"
     CDS             132..1100
                     /locus_tag="VBD11_001470"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6954417.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            1112..1492
                     /locus_tag="VBD11_001471"
     CDS             1112..1492
                     /locus_tag="VBD11_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6954418.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            1724..3565
                     /gene="typA"
                     /locus_tag="VBD11_001472"
     CDS             1724..3565
                     /gene="typA"
                     /locus_tag="VBD11_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6954419.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            3610..3855
                     /locus_tag="VBD11_001473"
     CDS             3610..3855
                     /locus_tag="VBD11_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6954420.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            3985..5340
                     /locus_tag="VBD11_001474"
     CDS             3985..5340
                     /locus_tag="VBD11_001474"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6954421.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            5343..6419
                     /locus_tag="VBD11_001475"
     CDS             5343..6419
                     /locus_tag="VBD11_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6954422.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            6423..7559
                     /locus_tag="VBD11_001476"
     CDS             6423..7559
                     /locus_tag="VBD11_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO6954423.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
BASE COUNT         2605 a         1192 c         1529 g         2325 t
ORIGIN      
        1 gatatcgaag ttatgaagat tgaattgcaa cgtctctacg ataatggtct gataagtaga
       61 gatgactatt taaaagcaga actgatttta agaagagagc acagactaga attggagaag
      121 gaaaataaaa aatgagtaag aaattattgg gaattgacct cggaggaacg accattaaat
      181 ttggtatctt gacgcttgag ggagaagtac aagaaaaatg ggcaattgag accaatactt
      241 tagaaaacgg aagacatatc gtttctgata tcgttgaatc tctcaaacat cgtttgagcc
      301 tctatggatt aacaaaagat gactttctcg gtatcggtat gggttctcca ggagctgttg
      361 atagaactag taaaacagta acaggtgctt ttaatctaaa ttgggctgat actcaagaag
      421 taggttcggt tattgaaaaa gaagttggaa ttccattttt tattgataac gatgctaatg
      481 ttgcagcact tggtgaacgc tgggtaggtg ctggtgccaa taatcccgat gttgttttcg
      541 taaccctcgg aacaggagta ggtggaggtg ttatcgcaga tggtaacctc atccatggtg
      601 ttgcaggagc aggtggagaa attgggcata tgattgttga tccagaaaat ggatttacgt
      661 gcacatgtgg taacaaaggc tgtcttgaga cagttgcatc agcgacaggt gttgttagag
      721 tagcacgtca actcgcagaa caatatgaag gttcgtctgc cattaaagca gcgattgaca
      781 acggtgatac tgttacaagt aaagatattt ttatagcagc agaagatggg gataaatttg
      841 ctaattctgt tgttgaacgt gtatcacgtt accttggact ggcagcagct aatatttcaa
      901 atattttaaa ccctgattct gtggttattg gtggcggtgt ctcagcagca ggtgaatttt
      961 tacgtagtcg cgttgagaaa tactttgtca catttgcttt cccacaagtt aaaaagtcaa
     1021 ctaaaattaa gattgctgaa ctaggtaatg atgctggtat tattggtgca gcaagcttag
     1081 ccaatcaaca agcaagttaa ggagataaga tatggatatg tctgttattt taattatcgt
     1141 tattcttctg gcttttgttg cttgggcatc ttggaattat tggagggttc gtagggcagc
     1201 taaattttta gataacgagt cttttcaaaa agaaatgtca cgaggacagt taattgatat
     1261 ccgagaagct ggtgcttttc atagaaagca tattctcgga gcacgtaaca ttccagcaag
     1321 tcaatttaaa gttgccttat cggctttacg caaagataaa cctgtcctct tatatgacgc
     1381 aagccgtggt cagtctattc caaggattgt tttgttactc agaaaagaag gttttaatca
     1441 actttacgtg ttaaaggacg gtttcaatta ctggacgggt agagttaagt aatatactaa
     1501 ggagagagtt actattaata actctcttct ctttttgtag aaatgaatta aattgaaaaa
     1561 agtagaaaat aaatgacata aataatgaaa tgatgaaaag ttttcctatc acaaataggc
     1621 agttaatatg aaaacattta cacttgtgta aattctgttt tttaagaaaa attgtgttat
     1681 aattcataag ttaacagaat tacattataa aatagaggaa aacatgacaa atttaagaac
     1741 agatatccgt aacgttgcga tcattgccca cgttgaccac ggtaaaacaa ctctcgttga
     1801 tgaattatta aaacaatcac atactcttga tgagcgtaaa gagcttgaag aacgtgcaat
     1861 ggattcaaat gatatcgaga aagaacgtgg tatcaccatt cttgcaaaaa atacagccgt
     1921 agcatacaac gatgttcgta tcaatattat ggacacacct ggtcacgcgg actttggtgg
     1981 tgaagttgag cgtattatga aaatggttga tggtgttgtt ttagtcgttg atgcttacga
     2041 aggaacaatg ccacaaacac gttttgtttt gaagaaagct cttgaacaaa acttaattcc
     2101 aatcgttgtt gtaaataaaa ttgataagcc atcagctcgt ccatcagagg ttgttgatga
     2161 agttcttgaa ctcttcattg agctcggtgc tgatgatgac caactagatt tccctgttgt
     2221 ttatgcttca gccatcaatg gaacatcttc aatgtcagat gatccttcag atcaagaaaa
     2281 aacaatggca ccgatttttg atactatcat tgatcacatt ccagccccag ttgacaactc
     2341 ggaagaacca cttcaattcc aagtttctct tcttgattac aatgattttg taggacgtat
     2401 tggtattgga cgtgttttcc gcgggactgt caaagttgga gatcaagtta ctctttcaaa
     2461 acttgatggt acaactaaaa acttccgcgt aacaaaactt tttggtttct ttggacttga
     2521 acgtaaagaa atccaagagg ctaaagcggg tgatttaatc gctgtttctg gtatggaaga
     2581 tatcttcgtt ggtgagacag taactccgac agatgctatt gaaccactac cagttttacg
     2641 tattgacgag ccaacacttc aaatgacttt cttggtgaat aattcaccat ttgcaggtcg
     2701 cgaaggtaaa tggattacgt cacgtaaggt tgaagaacgt cttttagcag aattacaaac
     2761 agacgtttct ttgcgtgttg acccaacaga ttcgccagat aaatggacgg tttcagggcg
     2821 tggagaatta catttatcta tcctcattga aacaatgcgt cgtgagggat atgaacttca
     2881 agtatcacgt ccagaagtta tcatcaaaga aattgatggt gttcaatgcg agccgtttga
     2941 gcgtgttcaa attgatactc cagaagaata tcagggtgct attatccaaa gtttgtcaga
     3001 gcgtaaaggt gatatgcttg atatgcagat ggttggtaat ggtcaaacgc gtttgatttt
     3061 cttgattcct gcacgtggtt tgattggtta ttcaacagag tttctttcaa tgacacgtgg
     3121 atatggtatc atgaatcata cttttgacca gtatctaccg gttgttcaag gagaaattgg
     3181 tggtcgtcat cgtggtgtct tggtttctat tgaaaatggt aaagcaacta catattcaat
     3241 tatgcgtatt gaagaacgtg ggactatctt tgtaaatcca ggtacagaag tttatgaagg
     3301 aatgattgtt ggtgagaatt ctcgtgataa tgatctcgga gtcaatatta caactgctaa
     3361 acaaatgaca aatgtccgtt cagcaactaa agatcaaact gcagtcatca agacgcctcg
     3421 tatccttact ttagaagagt cacttgaatt tttagcagat gatgagtaca tggaagtaac
     3481 tcctgagtct attcgtcttc gtaaacaaat tttaaataaa gcagcacgtg acaaagctaa
     3541 taaaaagaaa aaatcagctg aataatatcc aaagtatttc ttatttatga ttaataagaa
     3601 aggagttcta tgttttactt aattgttgcc cttttgattg ccttatatta cttttttatg
     3661 gcaccaaaaa cagtacgtaa tactttgaac gctattggtt tagtaggttt ggtagcctta
     3721 ttacttgtct tggcagttat gagttttatt aaaatccttc aattgccagg tgaattgtat
     3781 attgggctta taatgattcc tctaggatat acagcattta aagaaacatt gaatttgtca
     3841 gaaaagaaga aatagcttat gaacttagga ttttgtctcc taagttcttt tgttttgaga
     3901 aattagagac atatccttta ccttgaaaaa gccactgatt aaaggtaaaa taatagggat
     3961 aataatgata gaatgggacg agtaatgaaa acaataacaa catttgaaaa taaaaaagtt
     4021 ttagtccttg gtttagcacg atctggagaa gccgctgcac gtttgttagc taagttagga
     4081 gcaatagtga cagttaatga tggcaaacca tttgatgaaa atccaacagc acagtctttg
     4141 ttggaagagg gtattaaagt ggtttgtggt agtcatcctt tagaattgtt agatgaggat
     4201 ttttgttaca tgattaaaaa tccaggaata ccttataaca atcctatggt caaaaaagca
     4261 ttagaaaaac aaatccctgt tttgactgaa gtggaattag catacttagt ttcagaatct
     4321 cagctaatag gtattacagg ctctaacggg aaaacgacaa cgacaacgat gattgcagaa
     4381 gtcttaaatg ctggaggtca gagaggtttg ttagctggga atatcggctt tcctgctagt
     4441 gaagttgttc aggctgcgga tgataaagat attctagtta tggaattatc aagttttcag
     4501 ctaatgggag ttaaggaatt tcgtcctcat attgcagtaa ttactaattt aatgccaact
     4561 catttagatt atcatgggtc ttttgaagat tatgttgctg caaaatggaa tatccaaaat
     4621 caaatgtctt catctgattt tttggtactt aattttaatc aaggtatttc taaagagtta
     4681 gctaaaacta ctaaagcaac aatcgttcct ttctctacta cggaaaaagt tgatggtgct
     4741 tacgtacaag acaagcaact tttctataaa ggggagaata ttatgtcagt agatgacatt
     4801 ggtgtcccag gaagccataa cgtagagaat gctctagcaa ctattgcggt tgctaaacta
     4861 gctggtatca gtaatcaagt tattagagaa actttaagca attttggagg tgttaaacac
     4921 cgcttgcaat cactcggtaa ggttcatggt attagtttct ataacgacag caagtcaact
     4981 aatatattgg caactcaaaa agcattatct ggctttgata atactaaagt tatcctaatt
     5041 gcaggaggtc ttgatcgcgg taatgagttt gatgaattga taccagatat cactggactt
     5101 aaacatatgg ttgttttagg ggaatcggca tctcgagtaa aacgtgctgc acaaaaagca
     5161 ggagtaactt atagcgatgc tttagatgtt agagatgcgg tacataaagc ttatgaggtg
     5221 gcacaacagg gcgatgttat cttgctaagt cctgcaaatg catcatggga catgtataag
     5281 aatttcgaag tccgtggtga tgaattcatt gatactttcg aaagtcttag aggagagtaa
     5341 ctatggggaa aaaaattgtt tttacaggtg gtggtacggt aggtcatgta acactaaacc
     5401 taatattaat ccccaaattt atcaaagatg gttgggaagt acattatatt ggtgataaaa
     5461 atggcataga acatgaacaa attaatcagt ctggacttga tatcacattt cactctatag
     5521 ctactgggaa actacgtcgc tatttttcat ggcaaaatat gttagatgtc tttaaagtag
     5581 gggttggcgt tcttcaatcg attgctatta ttgctaaact tagaccccaa gccttatttt
     5641 caaaaggggg atttgtttca gtacctcctg tagtagctgc acgcctatta aaagttcctg
     5701 tttttgttca tgaatcagat ttatcaatgg ggttagcaaa taagattgct tataaatttg
     5761 cgactattat gtatacgact tttgaacaat ctaaagattt aatcaaaact aagcatattg
     5821 gtgctgtaac aaaagtcatg gattgtaaaa agtcgtttga aaatactgac ttaactagta
     5881 taaaagaagc cttcgatcca aacctaaaga ccttactatt tattggagga tcagcaggtg
     5941 caaaagtatt caatgatttt attactcaaa cacctgagct ggaagaaaaa tataatgtta
     6001 tcaatatttc aggcgattct tctctcaatc gattgaagaa gaacctttat cgcgtggatt
     6061 atgttacaga cctttatcag cctttaatga atttagcaga tgtggttgtt acacgaggtg
     6121 gctctaatac tatttttgaa ttagtagcta tgaaaaaact tcatcttatt atccctcttg
     6181 gacgtgaagc gagccgaggt gaccaattag agaatgctgc ttattttgaa gaaaagggct
     6241 acgcattgca gttaccagaa tctgaattaa acataaatac acttgagaaa cagataaatt
     6301 tattaatttc taacagtgag agttacgaaa aaaacatgtc acaatcatct gaaataaaat
     6361 ctcaagatga attttatcaa ttattaattg atgatatggc aaaagtaaca aaaggataaa
     6421 acatgcctaa gaagaaatca gataccccag aaaaagaaga agttgtctta acggaatggc
     6481 aaaagcgtaa ccttgaattt ttaaaaaaac gcaaagaaga tgaagaagaa caaaaacgta
     6541 ttaacgaaaa attacgctta gataaaagaa gtaaattaaa tatttcttct cctgaagaac
     6601 ctcaaaatac tactaaaatt aagaagcttc attttccaaa gatttcaaaa cctaagattg
     6661 aaaagaaaca gaaaaaagaa aaaatagtca acagcttagc caaaactaat cgcattagaa
     6721 ctgcacctat atttgtagta gcattcctag tcattttagt ttccgttttc ctactaactc
     6781 cttttagtaa gcaaaaaaca ataacagtta gtggaaatca gcatacacct gatgatattt
     6841 tgatagaaaa aacgaatatt caaaaaaacg attatttctt ttctttaatt tttaaacata
     6901 aagctattga acaacgttta gctgcagaag atgtatgggt aaaaacagct cagatgactt
     6961 atcaatttcc caataagttt catattcaag ttcaagaaaa taagattatt gcatatgcac
     7021 atacaaagca aggatatcag cctgtcttgg aaactggaaa aaaggctgat cctgtaaata
     7081 gttcagagct accaaagcac ttcttaacaa ttaaccttga taaggaagat agtattaagc
     7141 tattaattaa agatttaaag gctttagacc ctgatttaat aagtgagatt caggtgataa
     7201 gtttagctga ttctaaaacg acacctgacc tcctgctgtt agatatgcat gatggaaata
     7261 gtattagaat accattatct aaatttaaag aaagacttcc tttttacaaa caaattaaga
     7321 agaaccttaa ggaaccttct attgttgata tggaagtggg agtttacaca acaacaagta
     7381 ctattgaatc aacccctgtg aaagcggaag atacaaaaaa taaatcaact gataaaacac
     7441 aaacacaaaa tggtcaggtt gcggaaaata gtcaaggaca aacaaataac tcaaatacta
     7501 atcaacaagg acaacagata gcaacagagc aggcacccaa ccctcaaaat gttaattagc
     7561 gataaaaaaa gactggattt atcagtcttt tttatacata tcaagtcagt catagattgc
     7621 tagtaaaaat tgatttcttg aaaaaaattt a
//
LOCUS       DATROY010000088         7585 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.089, whole genome shotgun sequence.
ACCESSION   DATROY010000088 DATROY010000000
VERSION     DATROY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            5..1210
                     /locus_tag="VBD11_001477"
     CDS             5..1210
                     /locus_tag="VBD11_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6954424.1"
                     /translation="MYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDSFLAQ
                     TTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSASLLGF
                     ATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLAILLL
                     ISSILSHILYSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGLFVPD
                     ILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFLAVLY
                     ESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEAFGAL
                     MLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVKIAFA
                     KEEQYEKIT"
     gene            1194..2036
                     /locus_tag="VBD11_001478"
     CDS             1194..2036
                     /locus_tag="VBD11_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6954425.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            2113..2940
                     /locus_tag="VBD11_001479"
     CDS             2113..2940
                     /locus_tag="VBD11_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6954426.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(2980..3336)
                     /locus_tag="VBD11_001480"
     CDS             complement(2980..3336)
                     /locus_tag="VBD11_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6954427.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(3338..3814)
                     /locus_tag="VBD11_001481"
     CDS             complement(3338..3814)
                     /locus_tag="VBD11_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6954428.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(3869..5019)
                     /locus_tag="VBD11_001482"
                     /pseudo
     CDS             complement(3869..5019)
                     /locus_tag="VBD11_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(5092..5640)
                     /locus_tag="VBD11_001483"
     CDS             complement(5092..5640)
                     /locus_tag="VBD11_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6954429.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(5708..6799)
                     /gene="serC"
                     /locus_tag="VBD11_001484"
     CDS             complement(5708..6799)
                     /gene="serC"
                     /locus_tag="VBD11_001484"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO6954430.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            6932..7567
                     /locus_tag="VBD11_001485"
     CDS             6932..7567
                     /locus_tag="VBD11_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6954431.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAHGTRITQ"
BASE COUNT         2405 a         1421 c         1194 g         2565 t
ORIGIN      
        1 cactatttat aaatgtcatc attgccgtta tctaatttat aaaagtttta aagatatctt
       61 tccttttgct ctcatttttt cttggattga aatccttcaa aaactcgtta tatcagatga
      121 ttctttttta gctcaaacaa caaagtccag tatttataaa aacgagtggt tatatacggt
      181 ttccaatact ctttatgatt tatctcattt actaatctac tttatggcta gttattttgt
      241 ggctagttta gtatggagag gcttatctaa ccaaactaaa gaaagttttt ctgcaagttt
      301 acttggcttt gccactgttt ggttgcttct aacttcagtt gttccaggta cagattatca
      361 ctatcctagc caacccattt ggcttatgct ggctttgatt tcgtatcttt ttatcttttt
      421 aactcgactg cttcaaaatc gacatcatat tttccagtat agtatgttag ctatactact
      481 attaatctct tctattttgt cacatatctt atatagttat cctaatattg atttagaata
      541 ccttatacaa aatcactttt ctcaatggct gggtgatggg ccacatcaat taattcctat
      601 tatgctttgg tctatcttag ctatttttct aaccttatca ggtctttttg taccagatat
      661 tttatttcgt ccttactcct atttttcagt agttagcgaa aatttaaatg ctgccttgag
      721 ccaacataca gataaaatcc cttatctgta taccttttat actgttaaaa atagctttgc
      781 tatgtttggc ggtattggaa tcctattatc cttattttta gcagttttat atgaaagtag
      841 aaaactacaa tcgaagaatt actataaatt gacattactg accttaactc ctcttatttt
      901 tgatcaaaat ttaccatttt tagtaggtct acctgtcatt ttacaaccaa tattattcat
      961 tccgatggta ttaacaacta tctttgcaga ggcttttgga gctcttatgc tctacctaaa
     1021 atttgtcgac cctgcagttt atactgtccc aagtggtaca cctagtctac tatttggctt
     1081 tcttgcttca aatggggatt ggcgttacct tccagtcact gcaattattc tagttgttgg
     1141 cttctttatt tatcgtccgt ttgtcaaaat cgctttcgct aaggaggagc aatatgaaaa
     1201 aattacttag atggcttcct cctgtacttt tcattattat ccttatagga atgactatct
     1261 taggtaagtc ctatatcaat aaagtaacag ctcacaaaat aaaactctat aactctcgaa
     1321 tgactcctac tattttaatt ccaggatcca gtgctactca agaacgattt aacagcatgt
     1381 tagcacagct caaccaaatg ggagaaaaac atagcgtttt aaagttaact gtcaaaaaag
     1441 acaatagcat tatctacaat ggacaaatta gcggcaatga ccacaaaccc tacattgtca
     1501 ttggatttga aaataatgaa gatggttata gtaacatcaa aaaacaaaca aaatggctac
     1561 agattgctat gaatgatctt cagaagaaat ataaatttaa acgttttaac gctatcggtc
     1621 attcaaatgg tggcttatca tggactattt tcctagaaga ttattacgac tctgatgaat
     1681 ttgatatgaa atcattgtta acaatgggaa caccttttaa ctttgaagaa agtaacacct
     1741 caaatcatac tcaaatgctt aaagatttaa tcagtaataa aggaaatatt ccatcaagtc
     1801 tcatggtata caatttggca ggaactaatt catatgatgg tgataaaatt gttccatttg
     1861 ctagtgtgga gactggtaaa tatattttcc aagaaaccgc taaacactat acccaactaa
     1921 cagtaactgg taataatgct acacattctg acttgcctga taatcctgaa gttatccaat
     1981 atgtcgcaga aaaaattctt aaaaatgaga aaggtaaatt accaaaacct cactaaagct
     2041 gtagaatagc aacgcctctg gtattttgat ataatatatt gcgaattaaa ctaaataaaa
     2101 aaggatatta ttatgaaact catttcatgg aatattgatt ccctcaatgc ggcattaaca
     2161 agtgaatcaa cacgtgcttt aatgtcacgc caagttattg atactttagt tgctgaagat
     2221 gccgacatca ttgcgattca agaaactaaa ctgtctgcca aaggacctac taaaaaacat
     2281 ttggaagtac ttgaaactta cttcccagaa tatgatttgg tttggcgatc atcagtcgaa
     2341 cctgctcgta agggctatgc tggtacaatg ttcttatatc gtaaagggtt aaatcctatt
     2401 gttagcttcc ctgaaatcga tgctcctact acaatggata atgaaggtcg cattattact
     2461 ctcgaattgg aaaactgcta tatcacacag gtctatactc caaatgcagg tgatggccta
     2521 aagcgcttag ctgaccgtca aatatgggat attaaatatg ctgagtattt agcaacattg
     2581 gatagtcaaa aacctgttct agcaacaggt gactataacg tagcccacaa ggaaatagac
     2641 ctagctaatc ctagcagtaa tcgtcgttct gctggattca cagctgaaga gcgccaaggc
     2701 tttacaaatc tactagccaa aggttttaca gatacttttc gttaccttca tggcgatgtt
     2761 ccaaatgttt atagctggtg ggcacaacga agtcgtacat ccaaaatcaa caatacaggg
     2821 tggagaattg attattggct aacaagtaac cgtgtcgcag ataaaattac caagtcagaa
     2881 atgattcatt ctggtgatcg tcaagatcat acaccaatta ttttggagat tgaactataa
     2941 accaaaaaaa gatgattgag ttataactca atcatctttt tatactaagt tgaggtcttt
     3001 atacttagta cggtagccaa tctgaagaat tttattatct ttaactagca atggacgctt
     3061 aataagcatt ccatccgatg ccaataagtc caaagcttgg tcaagagtta aatcatcaaa
     3121 tttatctttt aagcctaatt ctctgtaaga atttcctgaa gtattgaaaa actttttaag
     3181 gtcataaggc gaattctcta acaattcttt taataacgaa gctttgggag gattgctttt
     3241 tatatcaatc gcttcaaaag tcaagccgag ttcagttaac tctttttttg ctgaacgaca
     3301 agtggtacat ttggggtatt cataaaacgt atacatatta tttttccttt tcgtattcta
     3361 ataaccaaga ttttctttct aagccaccag cataaccagt taattgacca tatcttccta
     3421 aaacacggtg acaaggtata attagagaaa taggattttt acctattgct cctccaacag
     3481 cctgtgcaga ttgacaattt agctctttcg ctaattctcc ataagttttg gtatctccaa
     3541 aagggatttt ctgtaattcc tgccatactc tcgtctgaaa tgctgtccct ctcaatgata
     3601 atggaatact aggtaaattt tgctgtgaaa aataagcatc taaccacttc ttaccaagta
     3661 aagtatagga atttgacata ttaacaattt cctcttggtt gactgctagc atatcatatt
     3721 tttgcccaac aaaataaagc cctgataaac ctagattgtc tgctagaagg cgaatttctc
     3781 caagagggga ctgatagaat tcttgatata acatatgata agcataactt aaaaagccat
     3841 gaaaagcaat tcatgacttt ttagttcttt attgttgttt tgcaattaaa cgtacacgga
     3901 cgatattctc atcaccttca aattcttcaa ttaaagtaga aatcttgtta ttatccgttt
     3961 catctaaatc tattagtgtg taggcgtagt caccctttga acggttaata atgttatcaa
     4021 tatttatacc taacactgat acagcagttg aaattttagc cataatgtta ggaacattct
     4081 tgttaattaa agtaatacga aatggtgcgg tttgaatttg atgtacattg gggaaattaa
     4141 ctgaattagt gatttctcct gtctccataa aacaacgaat ggtttgactt gccataatag
     4201 cacaatttag ctctgcctca tctgttgatc cgcctacatg aggaaagact gtaattcctt
     4261 tttggtttaa taattctttg tctccaaaat cagtaatata gcgcttgaca acaccagttt
     4321 ctatcgcttc aaatagctct tggttattaa ctaattctgc acgagcaaag ttgataatcg
     4381 tagttccttt tttcatgatt gaaaaagctt ccgcatcaaa agtatgctta gtttcatttg
     4441 ttaagggaac atgaattgtg atatagtcac aagtttcaaa aatatcctta atctctttaa
     4501 ccctttgaac atggcttgaa atattccaag ctgtttcaat tgaaatatag ggatcataac
     4561 caagaactgt cattcctaag cgtctagcac attcgcaatt ctagctccaa tggcaccaag
     4621 gccgataact cccaattttt ttcctgcaat ttcattacca gcaaaagctt tctttcctgc
     4681 ttcaatttgt ttgggaatat ctgttccagt tagagtatta acccatcggt tagctcctaa
     4741 ataatcacga gctgaaagta ataaggcagc aatgaccgct tcttttacag cattagcatt
     4801 tgcacctggg gtattaaaca cgactattcc ctgtgcactt gcctcttcaa taggaatatt
     4861 atttgttcct gcacccgccc tagcaatagc tttgaagtta cttgggaaat cctgattatg
     4921 caaattttga ctacgaataa tataggcatc aggattttct gacatatccc catcaatctg
     4981 gaaacgattc cctaactctt gtaaaccaat ttgattaata ttattaaatg ttttgacact
     5041 aaggttatct ctttagtcat cattgaccaa aataacccct ttcttttttt cttacatggg
     5101 atttctttca tcaatctcac gatagatgct cgtttctccc ttttctttta tcttctgata
     5161 agccaatcgt accccatcta gaggtacttt accacaatat tgatagccta atttatttag
     5221 aatatgctgc atagtgacat ttttttcatg agtatcgcaa cgaaaatcag gtcctttatg
     5281 accttcaatt aacccttgaa ggaatgtctg tgctagtcct ctaccacgaa attgatttga
     5341 gatcgcaata cgatgaaatg taagatagcg atggttatca tggagccatt ttccctcata
     5401 aatagcatca taaacctctt catgaccatc aatgacagcc gcataagtcg ctagcatccc
     5461 atcttcaata ccaacccaag cataaccgtt caagatatca tcaataatat cgttacgatt
     5521 agggtatcca tcttcttttt gccattgatc acttcctgtt ttagcaattt cggcacgcgc
     5581 ttcctcaata agaagcataa tttggtcaat ttcatttgga aaagctaatc tgatttgcat
     5641 tatattaccc acttttatat attatagact ataataacgg tttaattttc tttcgtgata
     5701 acatttacta tgtattttct gcttcaaatg ttttcataaa atcaattaat tcgatgacac
     5761 cttgtttagg aaaggcatta taaaggctag ctctcatacc acctaccgaa cgatgtcctt
     5821 taatattttt aaatccacgt tctgtcgctt cctttacaaa tttttcatct aaatctttat
     5881 taatagttat gaaaggaatg ttacatagag aacgactctt tttatctctg acaggattac
     5941 tataaaattc tgaggaatca atataatcat agagaagtcc tgatttttca cggttagctt
     6001 tttccatagc atcaacacca cctagacttt tcacccattc aaagactaat ttagcaatgt
     6061 aaatactgta agcaggcggc gtattataaa gagaaccagc atctgactga accttataat
     6121 ccaacattga tgataaagtt ggttcctcat taatcatatc ttcacggata ataactaccg
     6181 ttacaccagc aggtccaata tttttttgag ctcctgcata aatcattgca aaatcttcta
     6241 ctttatattt tacagctaat atattagaag acatatccgc aataacaggc actccattag
     6301 ttttaggaat atcataaagc gatgttcctt caatggtatt atttgttgtc acatgaacat
     6361 aggcagcttt aggatcaatc tctttttcat caaactctgg aatataatca taaactgatt
     6421 cttctgatga agctaataga attggttcaa aaggaattgt ttttgatagt tttacagcct
     6481 ctgtataagc ttttttcccc cacgaaccag caacatgata gtaagcttta cgccctctgg
     6541 taatattcaa gggaatcata ctaaactgta aggatgcacc accttgtaaa aagattactt
     6601 tataattatc cggaattccc atcaaatctc ttaagtaacg ctcagcacct ttgataatat
     6661 catcaaactc tttggagcgg tgcgatacct ccaaaacact catactagac ccttgataat
     6721 taaggagttc agattgggct tttacaagca ctggttttgg taagactgca ggacctgcag
     6781 aaaaattata aattatcata taacacctcc atataattgt gtaagattat agcacagtat
     6841 tttgaatatt gtaaagattt ttttgattaa ttttgctttt tattcgtgat ataatggtaa
     6901 aggcgaacaa tcaagtttaa aggagtaatt tatgattttg agggaatttt gtgcagagaa
     6961 tttaacagac ttaaccagat tagataaagc tattatttca cgtgtcgagc tttgtgataa
     7021 ccttgctgtc ggaggcacta ctcctagcta cggtgttatc aaagaagcta atcaatattt
     7081 acatgaaaaa ggtattagtg tcgctgttat gattcgtccc cgtggaggaa attttgtata
     7141 caatgattta gaattacgta ttatggaaga ggatatccta cgtgctgttg agttagaatc
     7201 agatgctctt gttttgggaa tattaacttc aaataatcat attgatactg aagccattga
     7261 acaacttcta cctgctaccc aaggtttgcc acttgttttt catatggcat ttgatgttat
     7321 cccgaaatct gaccaaaaaa aatcaataga tcaattagtt gctttaggct ttacaaggat
     7381 tctcctacat ggttcatcta atggcgaacc aattatagaa aatatcaaac atatcaaagc
     7441 attagttgaa tatgctaata atcgtattga aattatggtt ggcggcggtg tgactgctga
     7501 aaactatcaa tatatttgcc aagaaactgg ggttaaacaa gcccatggta ctagaataac
     7561 tcaataaaaa attcgaaaca gggct
//
LOCUS       DATROY010000089         7580 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.090, whole genome shotgun sequence.
ACCESSION   DATROY010000089 DATROY010000000
VERSION     DATROY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..781)
                     /locus_tag="VBD11_001486"
     CDS             complement(<1..781)
                     /locus_tag="VBD11_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6954432.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALV"
     gene            967..1827
                     /locus_tag="VBD11_001487"
     CDS             967..1827
                     /locus_tag="VBD11_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6954433.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            1870..2601
                     /gene="yaaA"
                     /locus_tag="VBD11_001488"
     CDS             1870..2601
                     /gene="yaaA"
                     /locus_tag="VBD11_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6954434.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(2958..3575)
                     /gene="nrdG"
                     /locus_tag="VBD11_001489"
     CDS             complement(2958..3575)
                     /gene="nrdG"
                     /locus_tag="VBD11_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6954435.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(3648..4139)
                     /locus_tag="VBD11_001490"
     CDS             complement(3648..4139)
                     /locus_tag="VBD11_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954436.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(4148..5080)
                     /locus_tag="VBD11_001491"
     CDS             complement(4148..5080)
                     /locus_tag="VBD11_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6954437.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(5093..5236)
                     /locus_tag="VBD11_001492"
     CDS             complement(5093..5236)
                     /locus_tag="VBD11_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954438.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(5311..7509)
                     /gene="nrdD"
                     /locus_tag="VBD11_001493"
     CDS             complement(5311..7509)
                     /gene="nrdD"
                     /locus_tag="VBD11_001493"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6954439.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
BASE COUNT         2451 a         1511 c         1112 g         2506 t
ORIGIN      
        1 ctactaaagc ataataaata ccatcgttat cattatcctg agttacattt ccaattctct
       61 cttgtaaatc tttaggagca ttccccatga caaaaccttt acccacaatt tcaagcagat
      121 ttaaatcatt atagttatca ccaaacgcaa tagagtctgc aagagaaata tctaaatagt
      181 ctgctaaagt tttagcagat tttccttttt caatacctgg agccattatt tcaataaagt
      241 aaggagcaga ttgtgcgatt gataaatgtg gatattgagc tttcaaagtg ttctccaatt
      301 ctcccatcag agatggttct cccatcaaga gaagtttatg aatcctatct tgcttctcta
      361 actccatcaa actcatctct ttagattgta aaccaacaat ctcctcctct ttttggaccc
      421 actcattttc tttatcacag gaataccatt catgataacg atagacattc catgcaattt
      481 tcgaataatg cttgcttaca taagaacaaa cttgtaaggc aacttgacca tccattgtgt
      541 gggaagcaat tgttttttgt tgcatatctt gtattaaagc cccattatag gaaataatat
      601 aagaatctat tccaatttgt tccataatcg gagtaattgc ctcaggcatc ctagctgata
      661 tgggaacgaa agtagcccct tgagctacta tttgtttaat tccctgccgt gtcttaggtg
      721 ttactttaaa attactgtta attaaagtac catcaatatc actaaatatt agccttacca
      781 tattatcctc caaaatataa ttgtacgcgc ttactttagt ctaacactta aatttttgtt
      841 tcgcaaataa acttcgtctg gtctaatgag actactaata taagctatat ttcaaaacaa
      901 attagtttat ttctctcaat gaacggttta atatgttact atatagtaat aataggagga
      961 ttaactatgc cgtatccata caaagccaat cattccattg aatcaatcac cttaaaagta
     1021 aatgacctcg aaaacctcgt aaacttctat agcgatatca ttgggttaac tgttattgac
     1081 aaatcaagca ctagagcctt actaggggta aatcaaaaaa tccctcttat tatccttgaa
     1141 aaaactgaac ttgaaaagca tagtacctat ggcctttatc acactgctat tttagtacca
     1201 gatgaatacc atctcagtct agctctaaat catttattat ctcagcatat ccctctagaa
     1261 ggtggagctg accatggcta tagcaatgct atctatctaa gtgatcctga aggtaatgga
     1321 attgaaattt ataatgataa ggatatctct atgtgggata tcagagagag tggacaaatt
     1381 attggtatta ctgaaagact agatattgac aatttacttg acagccttgt caacgttcct
     1441 aataactata agctttctga aaaaactcgt attggtcata ttcatttgag tgttaaagat
     1501 gctaagatta gctcaaaact ctatcaaaat gtttttggcc ttgatgagaa atttgcaatc
     1561 ccgacagcta gttggattgc atccggtaac taccatcatc atctagcctt taataattgg
     1621 gctggtccaa atctttctaa aaaccaagaa aatagacccg gaatttcctt attgacaatt
     1681 gcttacaatg atgacaacct ttttagagat agcttaaaaa aagcgcaact ttaccaactg
     1741 actttccttg aaaaacaaga tcattattat attattgaag attttgatgg catccgaatt
     1801 aaagttgttc taaaaccaga gaattaacca tcacttgact tttgactctt cattttacat
     1861 aatgtcaata tgataaaaat tttgatccca actgcaaaag aaatgaaagt gtgtcaaaac
     1921 attgcaaggc ctaaactgag tgcacaaacg caaattataa ttgattattt ttctacttta
     1981 actgtttcag acttagagga tatctacaga attaatacaa gtgcagctcg ttgtgaggct
     2041 caaagatggc aagattttaa atgtaaacaa cttactctga atcctgctat taagttattc
     2101 aacggtctta tgtaccgcaa tatcaaaagg cacaacttat ccacaagtga agcacaattt
     2161 atggaaaatt ctgtttttat tactagtgct ctttatggca tcattcctgc tatgacacta
     2221 atatcccctc atcgtttaga ttttaacact aaaatcaaaa tcaacaataa ctctcttaaa
     2281 gttttttggc gtgaaaatta cgatactttt atgcagtcag atgacattat ggtttctttg
     2341 ctttctaatg aatttaaaac cgtcttttct cctaaagagc gtcaaaaact aatacatctt
     2401 aactttattg aagatcgaga tggccaatta aaaacccact ctaccatatc aaaaaaagca
     2461 agaggaaagt gtttaacttc tatgatggaa aataattgcc aaactctaga acacttaaag
     2521 caattacgct ttgatggctt ctgttatgat aatgaactct cagactctaa gcaattaact
     2581 tttgttaaaa aacagactta agtaactatt tatccacaat ttattcacac aaaaagcaag
     2641 agataatcgt atctcttgct ttttgacaaa cttgtaaggt ctaaaaacct ctctatatca
     2701 aggtttcaaa taatattaac aaaaaataat tgttatcaac aacttgtgga taactatggg
     2761 gatagacaga ggataatctg tttatataaa gttgattact taacagtaat gtcattgtat
     2821 atacctttag atttcaaaaa tacttgttaa attaacaata ataaacgtca acatcttgtc
     2881 acagactgtc aaatacttat aaacaagctg tggataaaca taactttcaa tttctattta
     2941 aaagttaatt tttgacacta aagtaaataa tctcgtttga cttgttcata actattgtca
     3001 ccatcgttta atccttccca aatcacgact tcaccttctt ttaaagattt ttgtacatca
     3061 atgattcttt gatttgaaga tcctctaaat tgtagcatta aatttcgctt actttgatca
     3121 aacctaccat ccactaaaat atcaatcaaa gataacatct ccaatttatc ttgagtctcc
     3181 agcatcattt cttcccaagt ataccccgtc catgaccaaa tatctttttc tggcaattca
     3241 cgtcggatac gctgaagaag tggcagcaaa atacctgtat ttaagaacgg ctctccacct
     3301 aataacgtta aaccttggac gtaaggttgt gccaaatctt tcataatttg atcttcaagc
     3361 tcttttgtat aagggattcc ggctcgaaag gaccatgtag cagtattata acacccttta
     3421 cagtggaaca tacatccagc tacgtagagt gaatttctaa caccttcacc atctacaaaa
     3481 ttaaaagctt tataatcaat gatatggcca tgacttaatt cttcgctttt ccactcccca
     3541 ggctttggag tattccaact tgcttcttct gacatttcta acctctctat tgatatgaaa
     3601 agaaccagaa ctaactggtc tttttattat tatcttaaag tactatctta ggttatccaa
     3661 taacgctctg tctgatgacg acaatcttct aaaataccac cgttagctaa tattactgac
     3721 ttgctggcat cattgtctac atggcatgtc acaagtattt cattgatatt ttttagatgt
     3781 gcttgctcaa ttcctagctt caactgttct ttagcataac ctttaccacg ctggcgaggt
     3841 cgtatggaat aaccaatatg tccacctttt tctaaaagag tatcatttag tcttaaacgt
     3901 aaatttaaga aacctatagc attattgtca ttgtcaaaag acacaaactg aatatatgga
     3961 acaaaattat caggaacacc tatccctaac tccatattta ggttatgatc tagccagtct
     4021 tcaaaatgcg tagttggttc ataaaaacca ccatccattg aactactttc tttttgaaac
     4081 tctttataca tggctaaaat ttcttcttta tcttctaaca ctgggcgccg taatttcata
     4141 ttgtttccta ttccattatc ttcaagttaa gcattccaaa aggagcattc gctttattaa
     4201 tctggtcaca aataaaatga ctcaataaac tagactttgg tgatatagga ttgacacctg
     4261 tagtgatagc ttgaataaaa gcatcaatca ttggttcaaa accacgtttc tctagagtgc
     4321 ttgcccaaga accaaaaccg atagcacgac ggtcaaagcc atcaataaca gaaagatttg
     4381 acaaatcatc taatgaatag gttacttctg gcgactctat ttcaattatt tctcgacgag
     4441 accccgactg caggttcatg cttgcttcta ttatctcact gtctgtttct agcgttactg
     4501 atacttgtaa tatcttattc ccatcacgtt tagtcacaaa gtatcctctt ttaacaacat
     4561 tatttgtcaa aaatagcgcc gtatctaaag gatggataaa catatcaaaa agcttatatt
     4621 gaaaatccgc tggtgcattg acagcatttt taaaggtgcg aatatggttt ttatcttcta
     4681 cttttttcat ttccataata cgtggtgcaa agcgacgatt aaaacctgcc attaaaaatg
     4741 ttttatgatc cttcgccaaa tcatacaatg ctttagttga agtataatct tctgtcaaag
     4801 gcttatccat aaaaactgga ataccctttt ttaaaaaaag ctttgctata tcaaagtgag
     4861 cactagtcgc gacatgaatg aagactccat ccaaaggatg ctcggctaga ctatctaaac
     4921 tgtcataagc tgtagaagaa ccaaaaagca tattaacttc ttctaaaatc tgtttttggc
     4981 gtgtaaaaag atgccagtgc acacctgtca cttgacgcat ataaggtaga taagctttct
     5041 gtgaaatacc tcctaaaccg acaattccaa gttgcaacat agcatcctcc ttctattttt
     5101 tctttgcctt ttccaaaaat tgtttcctta aatcttttag acgtgatttt tgattagcat
     5161 tctctccagt agagtgcttc tcatggaact tctgtacttg tgcttgccct ttgtaatcta
     5221 attggtattt ccccatatct taataactcc ttctatttta tcttcttatt ttaacatata
     5281 atagaccaga ggttttaata gaggttttaa ttaattacct tgatttttaa tacttgatcc
     5341 gttcatatgt tttacacgag cagagatttc cttatgacga ccattaacca tcggacgagc
     5401 ctgtggatta cctaagtaac cacatgtacg tttgacaaca tctactgtct tagggtcgct
     5461 gttaccacaa tttggacaag taaagcctcg atctgtcggt gtaaaatccc cttcaaattg
     5521 acattggtaa cacttatcaa ttggtgtatt agtacctaga taacctaccc gatcataagc
     5581 atagtcccaa actgcttcta aagcttttgg attctgttgc aaaacagggt attcacagta
     5641 gtgaataaat cctcctgatg cacccgtttc aggatagatt ttctcaaaat ctagtttttc
     5701 aaaaggtgtt ggattcttac gcacatcata atggaatgaa ttagtgtagt attccttatc
     5761 agtaatatca gttactatac caaatttttc agtatctaaa cgacaaaacc ggtcagtcaa
     5821 gctctccgat ggtgtcgaat aaactgaaaa atgataatca tactcatcag accaatctgc
     5881 acataactgc ttcatcttac gaacgatgtc tacagtaaat gctttagctt gatgattatc
     5941 ttcccagtca ccaccataaa aaacactcgc cacctcatat aaaccaatat aaccaagaga
     6001 caccgttgca cggcggtgat taaagagtcg atcaacattc tcatattttc ctaaacgttc
     6061 tccaaatgct ccatactggt aaagaatggg cgcatttgct gggatagcct ctttgacacg
     6121 ttccacacga taaaccaagg catctctagc aatactcata cgttcattaa atatctccca
     6181 gaatttatcc atatcaccgt tagactccat ggcaacacgc ggtagattaa ctgttacaac
     6241 tccaagattc atacgaccag atgttacatc ctgaccattt gcatctcgcc acccttgtag
     6301 gaaggaacga cagcccatag gggctttaaa agagcccgtt aagtcaataa ttttatcata
     6361 agaaaggaca tctggataca tccgttttgt cgcacactct aatgctaatt gtttaatatc
     6421 ataattgggg ctattttctt ctaaattaag acctttttta agcgtaaaaa ttaattttgg
     6481 aaaaatagca gtacgatgct cgcttcctaa accttgaatc cgaattttca aaatcgcttt
     6541 ttgaatttct cgttcaaacc aattagttcc caatccaaaa ccaagcgaag taaatggcgt
     6601 ttgcccattt gaagtaaata aagtattaat ctcatactcc aaagattgca tggcatcata
     6661 tatatctttt tgtgttttag cgcgcgcata gtcctcttgt ttatcttcaa taacccattc
     6721 ttttgcatct tttaaatgtt tttgataatt taactgtgca taaggagcta aaaactcatc
     6781 aatgcgatct gctgtacagc caccatattg acttgaagca acgttcgcaa taatctgtga
     6841 aatctgagca gtcgctgtct gaatagattt tggactctca acttctgcat taccaatctt
     6901 aaaaccattt gcaagcattc ccttaaaatc gattaaacag caatttgtca tgggagtgta
     6961 tgggctgtaa tctaaatcat ggtaatggat atcacccttt tgatgtgcat ttgctacatg
     7021 agaaggtaac attttcagac caattgattt ccctacaata cctgctgtta aatcacgttg
     7081 ggtattaaac acatcagagt ctttattagc attctcatta acaacagttt gatctttatt
     7141 aattaatttt ccaatagaaa aattgatatc tgtagcctgt gaacgctcaa aatcgcgttg
     7201 ggtacgataa ttgatatatt cttgtgcaat agcgtactca ttagcttcca ataatttatg
     7261 ttcaacgata ttttgtattt catatatctt gatgttatct ttaaaacgat tgtagatctc
     7321 agcgatgaca tgatctgcaa tcatctcaag tttagcctca actaaaggag acattttgat
     7381 aactttatta ctagctttta atagagccgt gtagatttta tacttatcaa agctgaccaa
     7441 acgcccgtca cgtttaatca ctttgatatc agattctgtc ataaatcgtt ctctttccaa
     7501 taccatcatg tgaaatatcc tccagtgaca attatagcat gcaaagaaaa aaaatcaata
     7561 tattgtgtca agttttttgc
//
LOCUS       DATROY010000090         7500 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.091, whole genome shotgun sequence.
ACCESSION   DATROY010000090 DATROY010000000
VERSION     DATROY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            263..1273
                     /gene="nrdF"
                     /locus_tag="VBD11_001494"
     CDS             263..1273
                     /gene="nrdF"
                     /locus_tag="VBD11_001494"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6954440.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            1274..1687
                     /gene="nrdI"
                     /locus_tag="VBD11_001495"
     CDS             1274..1687
                     /gene="nrdI"
                     /locus_tag="VBD11_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6954441.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            1689..3854
                     /gene="nrdE"
                     /locus_tag="VBD11_001496"
     CDS             1689..3854
                     /gene="nrdE"
                     /locus_tag="VBD11_001496"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6954442.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            3917..7084
                     /locus_tag="VBD11_001497"
     CDS             3917..7084
                     /locus_tag="VBD11_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6954443.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATHDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQMEKNLKPVDYKTPIFN
                     KALPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            7097..7486
                     /locus_tag="VBD11_001498"
     CDS             7097..7486
                     /locus_tag="VBD11_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6954444.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         2533 a         1202 c         1480 g         2285 t
ORIGIN      
        1 gtggtgatat atgttgaaag acacaaaact ttgtgttagt atactgttta agcagatcct
       61 taataaaggt aaccgacaaa aaagtttaac gtgacttaac aaaacagtgg ttttgaagca
      121 agagttagct tcttaccaga ataaaaaaac atcaagaaga acattccaat agttgtgtga
      181 ttttaacaaa aatccagtcc tcttgggagg cttttttggt gcttaaaact cgcttaattt
      241 taatatttag gagactagtt taatgcaatc atactatgat cgttctcaat caccacttga
      301 ctatgccttg tcagaaaaag cttttcctat gcgttctgtt aattggaata agctaaacga
      361 tgacaaagac ttagaggtat ggaacagagt aactcaaaat ttctggttac ctgaaaagat
      421 cccagtttct aatgacctta attcttggag aacattggat gctgattggc agcaattaat
      481 tactcggact tttactggtc taaccttatt agattctgtg caggctactg ttggtgatat
      541 tgctcagatt aagcattctc agacagatca tgaacaagtt atttatgcta actttgcctt
      601 tatggtagct attcatgcgc gatcttatgg aacgattttt tcaaccttat gcactagcca
      661 acaaattgag gaagcacatg aatgggtagt ggatactgaa agtctacaag ctcgatcacg
      721 tatacttatt cctttttata ctggtgacga tcctttgaaa tctaaggttg ctgcagccat
      781 gatgccagga tttttgttat atggtggctt ttaccttcct ttttaccttt cagccagagg
      841 aaaattacca aatacgtctg atattattcg tttaatttta cgagataagg taattcataa
      901 ctattacagt ggctataagt atcaacagaa agttgctaaa ttatctgtag aaaagcaagc
      961 cgaaatgaag acatttgtct ttgacctcct ctatcagttg attgatttag aaaaagccta
     1021 tctttacgaa ctatatgatg gttttgattt ggctgaagat gcaatcagat ttagcattta
     1081 caatgctggt aaatttttac aaaatttagg atatgattct ccatttacag aagaagagac
     1141 tcgtatatct cctgaggttt ttgcacaact ttcggcgcgt gcagatgaaa atcatgattt
     1201 cttctcagga aacggctctt catatattat ggggattaca gaagaaacat tggatgaaga
     1261 ttgggagttt taaatggata gtacagtgaa ggtggtctat ttttcatcga aatctaacaa
     1321 tacccatcgt tttgttcaaa agttggcttg ttctaaccag cgtattccat cagatggtag
     1381 tagtatattg gttactgagg attatatttt gattgtgcct acttacgctg gaggaggaga
     1441 tgataccaaa ggcgcagttc caaaacaagt tgtacagttt ttaaatgttc gacaaaatcg
     1501 ggaacattgc caaggggtta tttcttctgg aaataccaat tttggtgaca cttatgccat
     1561 tgctggacct attattgctc gaaaattaaa tgttcctttg cttcatcaat ttgaattact
     1621 gggaacacaa gaagatgtta caagggtaaa ggagttactt tgccaattca ctagaaagga
     1681 taaataagat gacacaatca gatgcatatc tctcgttgaa cgcgaagaca cgctttagag
     1741 atcgcacagg taattatcat tttacttcgg ataaagaggc tgttgaacaa tatatgatag
     1801 aacatgttga acctaatacg atggtgttca catcactaat tgaaaagcta gattatttgg
     1861 tttctaataa ctactatgaa tcggaccttc taaaacaata taaccttgag tttatttgcc
     1921 aaatttttga gcatgcatac gctaagaaat ttgcttttct aaattttatg ggggctttaa
     1981 aattttataa tgcttatgct cttaaaacag aagacaatcg ttattatttg gaacactatg
     2041 aagatcgtgt tgtaatgaat gctctgtttt tagctgctgg tgatgaaaaa gctgcctatg
     2101 acctagtaga tgacatgcta gcaaatcgct ttcaaccagc aacacctacc tttttaaatg
     2161 cagggaaaaa acgtcgtggt gaatatatct cttgctattt gttgcgcatt gaagacaata
     2221 tggaatctat ttctcgtgct attagtacaa gccttcagtt atcaaaacga ggtggtgggg
     2281 tagcactttg cttaaccaac ttacgggaat ttggtgctcc aataaaaggt attaaaaatc
     2341 aagctactgg tattgtacct gtcatgaaat tactagaaga ttcgttttct tacgccaatc
     2401 aattaggaca aagacaagga gctggtgcag tttacttaca cgctcaccat cctgaagtat
     2461 taacgttttt agatactaaa cgtgagaatg ccgatgagaa aattcgaatt aaatctcttt
     2521 ccctaggact tgttattcct gatattactt ttgaattggc taaggcaaat aaagatatgg
     2581 ccttgttttc accgtatgac attgagcgag tatatggtaa accaatgtct gatatctcta
     2641 tcacagaaga atatgaaact ttattagcca atgcggatat tcgtaaaacc tttattagtg
     2701 cacgaaaact atttcaaacg atagcagagc ttcattttga atctggttat ccttatatct
     2761 tatttgaaga tactgtaaat gctaaaaacc ctcacaaaaa agagggacga attgttatgt
     2821 ctaatttatg ttctgagatt gcacaagtta atacagctag tcaattttca gaagacctga
     2881 ctttcactaa ggttggacat gatgtttgtt gtaatttagg ttctattaat attgcgcgtg
     2941 ctatggatca agcggcggat tttgaaaaat taattgctaa tagcattaga gcccttgatc
     3001 gtgtatctcg aacttctgat ttagatagtg ctccatctat taaaaaaggt aatgcagcta
     3061 atcatgctgt tggtttaggt gctatgaatt tgcatggatt tttggcgact aaccatattt
     3121 attacgattc tcaagaggct attgatttta cagattgctt cttttacgct atggcttatt
     3181 atgcctttaa agcttctaac catttagcaa aagaaaaagg aacctttgag ggcttctcgg
     3241 aatcaagtta tgctgatggc agttatttct atcaatatac cgaacaaaac tttgagccaa
     3301 aaacacagcg tgttaaaaat ctactagctg agtatggtct gactctacca agccaagagg
     3361 attggagaaa gcttgtgcaa agtattaaag agataggatt agctaatgcg catttattag
     3421 ctgttgctcc gacagggtca atcagttatc tttcttcttg tactccgagc cttcaaccgg
     3481 ttgtatcacc tgtcgaagta cgcaaggaag gagcactggg gagggtttat gtacctgctt
     3541 ataagattga tgcagataat tatgtctact acaaaaaagg agcttatgaa gtgggatctg
     3601 aggcgattat caatattgca gctgccgctc aaaaacacat tgatcaagct atttcgttaa
     3661 cgcttttcat gacagatcaa gcaactacgc gagatttaaa taaagcctat attcaagcat
     3721 ttaaacaaaa atgtgcctct atttattatg tacgagtgag acaggacatc ctagaaggta
     3781 gcgagagtta tgatgatatg ctggatgatt tcacttcatc ggacttagaa gactgtcaat
     3841 cctgcatgat ttaactaaat atactcaatc agaagattaa gagaaatata ttggctttta
     3901 gaaaggcttt attgtaatga caaaaaaaca tcttaaaacg cttgccttgg cacttactac
     3961 agtatcagta gtgacataca gccaggaggt atatggatta gaaagagagg aatcggtcaa
     4021 acaagaacaa acccagtcag cttcagaaga tgattggttc gaagaagata atgagaggaa
     4081 aacaaatgtt tctaaagaga attctactgt tgatgaaaca gttagtgatt tattttctga
     4141 tggaaatagt aataactcta gttctaaaac cgagtcagtg gtaagtgacc ctaaacaagt
     4201 ccccaaagca aaaccagagg ttacacaaga agcaagcaat tctagtaatg atgctagcaa
     4261 agtagaagta ccaaaacagg atacagcttc aaaaaaggaa actctagaaa catcaacttg
     4321 ggaggcaaaa gatttcgtaa ctagagggga tactttagta ggtttttcaa aatctggaat
     4381 taataagtta tctcaaacat cacacttggt tttaccaagt catgcagcag atggaactca
     4441 attgacacaa gtagctagct ttgcttttac tccagataaa aagacggcca ttgcagaata
     4501 tacaagtagg ctaggagaaa atgggaaacc gagtcgttta gatattgatc agaaggaaat
     4561 tattgatgag ggagaaatat ttaatgctta ccagttgact aagcttacta ttccaaatgg
     4621 ttataagtct attggtcaag atgcttttgt ggacaataag aatattgctg aggttaacct
     4681 tcctgagagt ctcgagacta tttcagacta tgcttttgct cacatgtctt taaaacaagt
     4741 aaagttacca gataacctaa aggtcattgg agaattagct ttttttgata atcagattgg
     4801 tggtaagctt tacttgccac gtcacttgat aaaattagca gaacgcgctt tcaaatctaa
     4861 tcgtattcaa acagttgaat ttttgggaag taagcttaag gttataggag aagcaagttt
     4921 tcaagataat aatctgagga atgttatgct tccggatgga cttgaaaaaa tagaatcaga
     4981 agcttttaca ggaaatccag gagatgaaca ttacaacaat caggttgtat tgcgcacaag
     5041 gacaggccaa aatccacatc aacttgcgac tgagaatact tacgtcaatc cggacaaatc
     5101 attgtggcgt gcaacacatg atatggatta taccaaatgg ttagaggaag attttaccta
     5161 tcaaaaaaat agtgttacag gtttttcaaa taaaggctta caaaaggtaa gacgtaataa
     5221 aaacttagaa attccaaaac aacacaatgg tattactatt actgaaattg gtgataacgc
     5281 ttttcgcaat gttgattttc aaagtaaaac tttacgtaaa tatgatttgg aagaaataaa
     5341 gctcccctca actattcgga aaataggtgc ttttgctttt caatctaata acttgaaatc
     5401 ctttgaagca agtgaagatt tagaagagat taaagaggga gcctttatga ataatcgtat
     5461 tggaactcta gacttgaaag acaaacttat caaaataggt gatgctgctt tccatattaa
     5521 tcatatttat gccattgttc ttccagaatc tgtacaagaa ataggacgtt cagcttttcg
     5581 acaaaatggt gcgcttcacc ttatgtttat cggaaataag gttaaaacaa ttggtgaaat
     5641 ggctttttta tccaataaac tggaaagtgt aaatctctct gagcaaaaac aattaaagac
     5701 aattgaggtc caagcttttt cggataatgc ccttagtgaa gtagtcttac cgccaaattt
     5761 acagactatt cgtgaagagg ctttcaaaag gaatcatttg aaagaagtga agggttcatc
     5821 tacattatct cagattactt ttaatgcttt tgatcaaaat gatggggaca aacgctttgg
     5881 taagaaagtg gttgttagga cacataataa ttctcatatg ttagcagatg gtgagcgttt
     5941 tatcattgat ccagataagc tatcttctac aatggtagac cttgaaaagg ttttaaaaat
     6001 aatcgaaggt ttagattact ctacattacg tcagactact caaactcagt ttagagaaat
     6061 gactactgca ggtaaagcgt tgttatcaaa atctaacctc cgacaaggag aaaaacaaaa
     6121 attccttcaa gaagcacaat ttttccttgg tcgcgttgat ttggataaag ccatagctaa
     6181 agctgagaag gctttagtga ccaagaaggc aacaaagaat ggtcatttgc ttgagaggag
     6241 tattaacaaa gcggtattag cttataataa tagtgctatt aaaaaagcta atgttaagcg
     6301 cttggaaaaa gagttagact tgctgacaga tttagtcgag ggaaaaggac cattagcgca
     6361 agctacaatg gtacaaggag tttatttatt aaagacgcct ttaccattgc cagaatatta
     6421 tatcggattg aacgtttatt ttgacaagtc tggaaaattg atttatgcac ttgatatgag
     6481 tgatactatt ggcgagggac aaaaagatgc atatggtaat cctatattaa atgttgacga
     6541 ggataatgaa ggttatcata ccttggcagt tgccacttta gctgattatg aaggtcttta
     6601 tattaaagat attttaaata gttcccttga taagattaaa gcaatacgcc agattccttt
     6661 ggcaaaatat catagattag gaattttcca agctatccga aatgcagcgg cagaagcaga
     6721 ccgattgctt cctaagacac ctaaggggta cctaaatgaa gtcccaaatt atcgtaaaaa
     6781 acaaatggag aaaaatttaa aaccagttga ttataaaacg ccgattttta ataaggcttt
     6841 acctaatgaa aaggtagacg gtgatagagc ggctaaaggt cataatataa atgcggagac
     6901 taataattct gtagctgtaa caccaataag gtccgagcag caattacata agtcacagtc
     6961 tgatgtaaat ttacctcaaa caagttctaa aaataatttt atatacgaga ttctaggata
     7021 cgttagttta tgtttgcttt tcctagtaac tgctgggaaa aaaggaaaac gagcaagaaa
     7081 ataaggagaa attaatatga ttacacaaga aatgaaagag attattaata gtcagttagc
     7141 aatggtcgcg acggttgatg ctaaaggtca acccaatatt ggcccaaaac gttctatgcg
     7201 tctctgggat gataaaacgt ttatttataa tgaaaataca gatggtcaaa ctcgtattaa
     7261 cattgaagat aatgggaaaa ttgaaattgc ctttgttgat cgtgaacgtc ttttaggtta
     7321 caggtttgtg ggaactgctg aaattcaaac agaaggtact tattacgaag cagcgaaaaa
     7381 atgggcagaa ggacgcatgg gggttccgaa agcagttggt attattcatg tcgagcgtat
     7441 ttttaattta caatctggtg ctaatgcagg taaagaaatc acttaatata aaaaagcctc
//
LOCUS       DATROY010000091         7441 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.092, whole genome shotgun sequence.
ACCESSION   DATROY010000091 DATROY010000000
VERSION     DATROY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            28..327
                     /locus_tag="VBD11_001499"
     CDS             28..327
                     /locus_tag="VBD11_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6954445.1"
                     /translation="MLELKFQQERMLKGLHIKNTDQLIFYDYRFGMISNDAVNKFLAS
                     SLEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQLIETYGHLLL"
     gene            complement(463..533)
                     /locus_tag="VBD11_001500"
     tRNA            complement(463..533)
                     /locus_tag="VBD11_001500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(499..501),aa:Thr,seq:ggt)
     gene            complement(604..1438)
                     /locus_tag="VBD11_001501"
                     /pseudo
     CDS             complement(604..1438)
                     /locus_tag="VBD11_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            1749..2003
                     /locus_tag="VBD11_001502"
     CDS             1749..2003
                     /locus_tag="VBD11_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6954446.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            2112..2423
                     /locus_tag="VBD11_001503"
     CDS             2112..2423
                     /locus_tag="VBD11_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954447.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            2691..3260
                     /locus_tag="VBD11_001504"
     CDS             2691..3260
                     /locus_tag="VBD11_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954448.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            3358..3942
                     /locus_tag="VBD11_001505"
     CDS             3358..3942
                     /locus_tag="VBD11_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954449.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            3939..4505
                     /locus_tag="VBD11_001506"
     CDS             3939..4505
                     /locus_tag="VBD11_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6954450.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            4505..7156
                     /locus_tag="VBD11_001507"
     CDS             4505..7156
                     /locus_tag="VBD11_001507"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6954451.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
BASE COUNT         2388 a         1292 c         1530 g         2231 t
ORIGIN      
        1 tcagtaagtc atgatttaaa agagttattg cttgaactaa aatttcaaca agaaagaatg
       61 ctgaaaggtt tgcatataaa aaatacagat caattgattt tttatgacta taggtttggt
      121 atgatatcaa atgatgctgt taacaaattc ttggcaagct cattggaaaa attagaaata
      181 gaatctaaga tgtcatcaac aggtgctaga catacttatg gaagttattt gttggctaac
      241 ggagtagata tttgggcggt tgcaaaatta atggggcata aagatatcaa acaattaata
      301 gaaacatatg gtcatttgct tctgtaagtg gaagaaaagg aaaatgatca gcttttaaaa
      361 attcttaata ataaaggtag tttttaaaat gactatcaag aacaaattaa gaacaaaaat
      421 aaaagaaacc ctattataat aaggtttcac tcatatattt tctgccacca ccgagaattg
      481 aactcggaat tgagcattac cattgctcta ttataccatt taactatagc ggctactttt
      541 ctatgatact ataagatttt gacgaaatca agtatttctt tacttagaaa aatagtcgtt
      601 tttttagtct aattctattt ggagaaattt actattttca atagctctta aatggtgtgt
      661 ttcttgtgga gagaggctaa taaggctatc tttttcaagg atatgtttct gatcaccaag
      721 gataatttca agttttcttt ccaaaacatt tattaatttt ggatgagggc atcgttcatt
      781 tttcagtccc cggctgatga ttttaatggt attgaataaa ccttcatctt taatcattcc
      841 taacggggac ataagactta tttttccaaa ttgatattct gaactttaaa aaccattttg
      901 taagaataaa ttttgccaat catctatttt tagaggagca acgttaacat tcatagtatg
      961 tctaagttga tcgatcagct tggcagtgtc ttcatcttga taagaaacat catgtgtgag
     1021 taaacgtcca ccaggtttta ggacacgtaa gtactctttt atggctttct ccttagctga
     1081 attgttaagc atcgttaaca ttgcttcgtt gataacgata aactattatc aggaaagggc
     1141 agtctgagtg catttccctg aataacttca ataaattctt ctaattgggc ctttttgaca
     1201 ttttctttgg ccttttctat tactttacta tctagatcaa gcgctgtaat gtgacaggaa
     1261 aatgctttag cgatatcgat ggaactggta cattgattac aagctacttc tagaacacga
     1321 ctgtcagcac taaagtgtcc ttttttgata agccaattcg ttgcttggac acctcctggt
     1381 cttaagcgtt ttttcctagt cgtgctaaga attgatgtcc aatttcttta tgtggcaata
     1441 agatcacctc tttcttttaa tagtaatatc attttttctt aattataaca catagatgct
     1501 aagataacaa ggagtaatag tgattgaatg gtataagtga tgaattttag agagcgtttt
     1561 cttttaacta ggggtgtgat gccaaaacta gaatggcata gagaaccata catctcaaaa
     1621 ggtcttcctt agataaaact agttgttttt atatctgtca gaaaaatcat aatttatttt
     1681 tgatataatg attttttgag tatttaattt atatgttata atgaaaaaaa ctaaaaaaga
     1741 ggaatatcat gtcatacgaa caagaatttt taaaagactt tgaagagtgg ttacaatcgc
     1801 aaataagtat caaccaaatg gcaatggaca gtgcaaagaa agttttagag gaagataagg
     1861 atgagcgagc agcagatgcg tatattcgtt atgagagtaa gttagacgct tatcgctttt
     1921 tgcaagggaa atttaataac tatcataacc aaaaatcttt ccatgatttg cctgatggtc
     1981 tttttggaca acgtcattat taaaaggttt atcgctagaa agcttgtcaa ataggcattt
     2041 ctatgataca ttgttatagt agtttttaga tactatgata gatttcataa atgaggagaa
     2101 agcattatac aatggcaaag cgtattaaac cgaataaaaa aagagagctt ttgaaagcta
     2161 aagggatcaa ttggaaggat gttcgactat ctcaaacttt acaagaagtc gcatcaccga
     2221 aaaaaaaggc tcctgaagcg ccaaaggcag tcgatctttt agaaggttta actgctaata
     2281 aacaagatgt tttaaaagaa gctggccttg tcagccttga agcgtttgcg aaagtaagtg
     2341 aagcagatgt tttagcacta aaaggtattg gtccagcagc tattaaacag ttagttgata
     2401 atggtgctgt ttttgcaaaa tgatttgatt caccttgcga aagtagtctt attttgctag
     2461 aataaattaa cttagaggtg ttttgaaatc ccatttgtca caggaagcgg tcatactgag
     2521 aaatccgcac aaatgtcaaa gcttgttgct aagtccaata attgaaacaa aacgagttgc
     2581 ggatgttatc atccgaattt gggtggtacc gcggaaatcg taatacgaaa ttcgtccctg
     2641 tcagaattga cagggatttt ttgtatttag gagtgaggag attgaagtat atgcagattc
     2701 tagaagattt cgatggcaaa gcattaccaa aactagaaac ggatcgactt atcttgcgtc
     2761 agcgaacggt gggggatgtc ccagccatgt ttgattacgt ttgcttggaa gaggttgcct
     2821 atccagcagg gctcagtcca atagcttctt tagaagatga gtatgattat tttgaaaatc
     2881 gttactatca aaatctggaa aaggcaaaat taccatctgg ttatggtatt accgttaaag
     2941 gaagtgaccg gattatcggc tcttgcgact ttaaccaccg ccatgaagac gatgttttcg
     3001 agattggcta catgcttcat cctgactatt ggggtcatgg ttatatgaca gaagctgtcg
     3061 cagccttgat tgaagtcggt tttaccctcc tcaatctcca caaaatcgaa atccgctgct
     3121 atgattacaa taagcaaagc cggcgcgtgg cagaaaaact aggcttcacc cttgaagcca
     3181 ccatccgtga tcgtaaggat aatcaaggta atcgctgtgt caatttgata tatggtttgc
     3241 tgaggagtga gtgggagtaa ttattctact cttcatagaa gttactagat agactaattt
     3301 ggctgtgcag ggtacttagc taactcatca ataatatcgt agatatggag gagacaaatg
     3361 attaagatac ttggagaaat cgtagataac cagctacctg ttgtggaaac gaatcgtctg
     3421 ttgttgcgtc aacgcaagtt agaagatgct aaggagatat ttgaatttgt caaacttgat
     3481 gaggtgactt atccagcagg ttttccggct gttaaaagct tggaagaaga gataacctat
     3541 attcaagaga tttatccaac taatcttgaa aaagaaaagt taccctctgg ttatgctatt
     3601 actctaaaag gcgatgataa ggtaattggt tcagtagatt ttaaccatcg ccacgaagac
     3661 gacatttttg aaattggcta cctgctccac cctgattatt ggggacaagg cattgtgcca
     3721 gaagcagctt cagtccttgt ggagataggt ttcacactgc taggactaca taagattgag
     3781 ctgggatgct atgactataa taagcaaagt caggcggttg cgcgcaaatt aggttttact
     3841 ttagaggcta atattcgtga tcgaagagat gcccaggggc aacgttgtgg cgatatgcgc
     3901 tttggtttac tgcgaagcga gtgggagaaa aaaagaagat gaatattatt atcatcggag
     3961 cacaagcttc tgggaaaatg acaattggtc aggaaatcgc aaaacagaca ggcatgacct
     4021 tattccttaa ccatgattct atagattttg ttttacgctt catgccatgg tctccagata
     4081 gtattgctct aacggagtct attcgcttta aattttttga aacgtttgct aagactggtc
     4141 aagaaatgat ttttactatt gtaattgatt tcaatgattc aagagatgta gcatttctag
     4201 aaaagattca aacagttttt caatctcaca accaagaggt attatttgta gaattggaaa
     4261 cagagctaag tgagcgtctc aaacgaaatc gcacagaaaa tcacctcaag cataagcctt
     4321 caaaacgtga tatcaaatgg tcagagaacg atatttgttc aaccatggat tacgctatct
     4381 ttaatcctga agtagcccca gaagcgctta catattatta taaaatcaat aatacttgct
     4441 taactgctac tgaaacagca tatcttatta ttcaaaaaat aaatcaaatc aaggagaaaa
     4501 actaatgtca aaagaactat caccaaaata caatcctgcc gaggttgagg caggccgtta
     4561 tcaaacttgg cttgaccaag atgttttcaa gccatcaggg gatgctgagg ctaagcctta
     4621 ttctattgtg attccaccac caaacgttac gggtaaattg cacttgggtc acgcttggga
     4681 tacaactcta caagatatca ttattcgtca aaagcgtatg cagggctttg atacactctg
     4741 gcttccaggt atggaccacg ctggtatagc cactcaagct aaagttgagg agcgccttcg
     4801 tgagcaaggc atttcacgtt atgatcttgg tcgtgaaaaa ttcctagata aagtttggga
     4861 atggaaagac gagtatgcag caactattaa atctcagtgg gggaaaatgg gtctttcggt
     4921 tgattatagc cgtgagcgtt ttacccttga tgagggactt tcaaaagcgg taagaaaagt
     4981 ctttgttgat ttgtacaata aaggttggat ctatcgtggg gagttcatca ttaactggga
     5041 tccagcagct cgtacagccc tttcagatat tgaagttatt cacaaggatg ttgagggtgc
     5101 tttctatcat atgaattata tgctggaaga tggctcacgc gccctagaag tagcaacaac
     5161 tcgtcctgag actatgtttg gagatgtggc tgtagcggta aatccagaag atccacgtta
     5221 caaggatttg attggtcaaa atgttatcct tcctatcatc aataaaccta ttccaatcgt
     5281 cgctgatgaa cacgctgatc cagaatttgg aacaggtgtg gttaagatta cgccggctca
     5341 cgaccctaat gacttcgctg taggtcaacg ccacaactta ccacaagtta acgttatgaa
     5401 cgatgatggg actatgaatg aacttgcgga tgaatttaat ggtatggatc gtttcgaagc
     5461 tcgtaaggca gtagtcgcta aattagaatc gttaggaaac ttagtaaaaa ttgaaaaaat
     5521 gactcattct gttggtcatt cagaacgaac aggcgttgtg gttgaaccac gtttatctac
     5581 tcaatggttc gtcaagatgg atcaattggc taagaatgcc attgccaacc aagatacaga
     5641 agacaaggta gagttttatc caccacgatt taacgatact tttatgagct ggatggaaaa
     5701 tgtccatgac tgggttatct ctcgtcaatt atggtgggga caccaaattc ctgcatggta
     5761 caacgtcaat ggtgagatgt atgttggaga agatgcccca gagggtgacg gatggacaca
     5821 ggatgaagac gttctagaca catggtttag ttctgcttta tggccgtttt caacaatggg
     5881 atggccagac accgaagcag ctgacttcaa acgctacttc ccaacgtcaa ccttggtaac
     5941 tggatacgat atcatcttct tctgggtatc gcgcatgatt ttccaatctc ttgaatttac
     6001 aggacgccaa ccattctcaa atgttcttat tcatggactc atccgtgatg aagaaggacg
     6061 taaaatgtct aaatcacttg gcaatgggat tgatccgatg gatgtcattg agaaatatgg
     6121 tgcggatgct cttcgttggt tcctctcaaa tggttcagcc cctggtcaag atgtgcgttt
     6181 ttcatacgaa aaaatggatg cttcgtggaa tttcattaat aagatttgga acatttcacg
     6241 ctatatcctt atgaataacg aaggtttaac gttggatcaa gccagagaaa atgtggaaaa
     6301 agttgttaat agtcaagttg gaaatgtaac agaccgttgg attctccaca atttaaatga
     6361 gactgtgggt aaagttactg aaaactttga caagtttgaa tttggtgttg ctggtcacat
     6421 cctttacaac tttatctggg aagagtttgc taattggtac gttgagttaa caaaagaggt
     6481 tctttatagt gacaatgaag atgagaaagt tgttacacgt tctgttcttc tttatacctt
     6541 agatcaaatc ttgcgtcttc ttcatccaat catgccattc gtgacagaag aaatctttgg
     6601 tcagtatgca gatagctcaa tcgtaacagc tgcttaccct acggttaacc cagcctttga
     6661 aaatgctgct gcccacaagg gagttgaaag cttgaaggac ttgattcgtt ccgtgcgtaa
     6721 tagccgagca gaggtcaatg ttgctccatc taaaccaatc actatcttgg ttaagacaag
     6781 tgacagcgaa ctcgaaagct tcttcaaaga caatagtaac tacatcaaac gtttcacaaa
     6841 cccagaaaca cttgaaatca gctcagctat tgccacacca gaacttgcca tgtcatcagt
     6901 catcacagga gctgaaatct tcctcccact tgctgacctc ctcaacgtag aagaagagtt
     6961 agcacgactt gaaaaagaac tagccaaatg gcagaaagaa ctggacatgg taggcaaaaa
     7021 actcggcaac gaacgcttcc tcgccaacgc caaaccagaa gttgtccaaa aagaaaaaga
     7081 caaacaagcc gactaccaag ctaaatacga cgcaacccaa gaacggattg tagagatgca
     7141 aaaacttgtt aaatagttaa aatcatccat tacttccgca gagtcctgcc gctagaccat
     7201 cagtctaagc gacagggctt tttgctatat tcaaagattg tgaaaaatta atatatatga
     7261 tgcctacttc ttgcgtctga aatacaagaa gtgaaaatga tttggccata aagaaaaaca
     7321 ggcagaaacc caaatgatta atgcctattt cttatatttt ttcgtcaatt attttataaa
     7381 taaaattgag gtacaaacca gtttttaaaa tgataaggtg aatcttcttc aatatagatg
     7441 t
//
LOCUS       DATROY010000092         7432 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.093, whole genome shotgun sequence.
ACCESSION   DATROY010000092 DATROY010000000
VERSION     DATROY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            333..856
                     /locus_tag="VBD11_001508"
                     /pseudo
     CDS             333..856
                     /locus_tag="VBD11_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            927..2032
                     /locus_tag="VBD11_001509"
     CDS             join(927..1203,1203..2032)
                     /locus_tag="VBD11_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6954452.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            2256..3388
                     /locus_tag="VBD11_001510"
                     /pseudo
     CDS             2256..3388
                     /locus_tag="VBD11_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3428..3666
                     /locus_tag="VBD11_001511"
                     /pseudo
     CDS             3428..3666
                     /locus_tag="VBD11_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3865..6333)
                     /locus_tag="VBD11_001512"
     CDS             complement(3865..6333)
                     /locus_tag="VBD11_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6954453.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(6346..7266)
                     /gene="lmb"
                     /locus_tag="VBD11_001513"
     CDS             complement(6346..7266)
                     /gene="lmb"
                     /locus_tag="VBD11_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6954454.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         2301 a         1459 c         1343 g         2329 t
ORIGIN      
        1 tgaatataat tctttatcag acatcatata agatagagac tttttgtcca atgttgagcg
       61 gttaaataac tctttatgag gaatcattgc aagaactgat ttatcattct ttagtgatct
      121 tattaattct tttgaagagt tggtattatg ctttatcata ttacctaaaa agaataattt
      181 tgctgtgaca taacttgctc gagttttaaa gtcaaatgcc tctgttttaa attcttcaag
      241 tctttcagaa agatggatgt atactataaa agtagacaaa actttgtgtg ttacaatctg
      301 ttttaaaaga cacaaaaacg aaaggacaac ctatatcaac atttaaacgc tatgatgaag
      361 aattcaaaca atctcttgtt aacctttatc aaactggaaa aactcagtct gaactctgta
      421 aagactatgg ggtatccact tctacgcttg caaaatggat caaacagtat tcccaagtca
      481 aactcgaaga taattctgta cttactgcca agcaaataca agagttacaa aaacgtaatg
      541 cgcagcttga agaggagaac cttatcctaa aaaaagcaag tgccatattc atgcaaaact
      601 taaagtaaga ctcctcgctg tctatcggtt acgctttgaa cacgccacaa caaccttgtg
      661 tcgtgtttta cgtgtcaatc gctccactta ctataaattt ctaaaacata agccctcaaa
      721 aagagaattg gataatcaaa tttatagaaa acaaatactt gagatttata ccaaagcgaa
      781 caaaagactt ggtgtgaagt ctatcaaggt cattcttcaa agagactatg acacaaaaat
      841 ctctgaagga agaatttgaa taggttacac taaactagac agaaattata aagtgttcta
      901 cacttaagaa aaactggaga aaaaccatgt ctagaaaagt acgtcgtcac ttcaccgatg
      961 attttaagca acaaattgtt gacctttaca atgttggtag aaagcgtagc agtctcatca
     1021 aggtatatga gctaacccct tccaccttcg ataagtgggt tagacaagcc aaaacaactg
     1081 gttcattcaa gtctgttgat aatctgacag atgaacagcg ggagctaatt gaactcagaa
     1141 aacacaataa agaactcgaa atgcaattag atatcctaaa gcaagcagcg gtgattatgg
     1201 cacaaaaagg gaaataatca ctgctaataa ggataaatac agcatttcaa agatgtgtcg
     1261 ctggctgaat atgccacact caagttatta ctatcaagcc gtggagtcag tatctgaaac
     1321 ggagtttgaa gaaactatta aaagaatttt cctcgatagc gagtccagat acggatccag
     1381 aaaaatcaaa atatgcttga ataacgaagg tatcacactt tcacgtcgtc ggattcgacg
     1441 cattatgaag cgactcaatt tggtttctgt ttatcagaaa gccaccttca aaccacattc
     1501 tagaggcaag aatgaagccc ctattcccaa ccacttagac aggcaattta agcaagaaag
     1561 accactacag gccttagtca ctgacttaac ctatgttcgt gtaggcaatc gttgggctta
     1621 tgtttgcctc atcattgacc tatacaaccg tgaaatcatc ggcctgtctc ttggttggca
     1681 caagaccgct gaactcgtta agcaagccat acaaagcatc ccttacgccc tgaccaaagt
     1741 caagatgttc cattcagatc gtggcaaaga gtttgataat cagttaattg atgaaatatt
     1801 ggaagccttt ggaatcacac gttcgcttag tcaggctggt tgtccttatg acaatgccgt
     1861 agctgaaagt acgtatcgtg ctttcaaaat tgaatttgtt tatcaagaaa cctttcaatt
     1921 gctggaagaa ctagctctta agactaaaga ctatgttcat tggtggaatt accaccgcat
     1981 tcatggtagt ctcaactacc aaacaccaat gactaaaaga ttaattgcct agaaaaacac
     2041 tttataaatg ttgtacagaa aagtgttgcc ttttcactta ataaaagaaa gattgaagga
     2101 acacttctct ttcattccga ccaagggtca caatttaagt ccgggaaatt tagaaaaata
     2161 attgatgaca acaataggca ctgaatccct aaaatgagac aaaagaaaaa cactcctgtt
     2221 gaaatttagt aaactagaaa caggagtgtt ttattatggt taaaaaagca tattcatggg
     2281 aaactaagct ggcttgcatc gatatgaaga aagcaggcaa gtctaacaga gttattatgg
     2341 agactttagg catcaaaaat aatagtcaaa tctatacttg gatgaaatgg tacgaaaacg
     2401 aggagcttta tcgttttcat caaggtgttg gcaagcaata tacctacggt aaaggcttgg
     2461 aacatctgtc tgaagtggaa caattacaat tacaggttga cctcttaaaa aagtatcgag
     2521 gcttgataag gaaatcgata aagtaagtct tatcaagctt gtggaagact ataaaaacaa
     2581 ctacccgata gttttcattc tagactgttt taaggtcaag cggtcgacct attaccgttg
     2641 gaaaaaggag tatgagaagc cgcaaaaaaa gatgacctta ttgaattaat tgaacagctc
     2701 tgtatggaga atcactttat ttatggctat cgtaccatta cacgattgct caaaaaaatc
     2761 cacggattga ctgttaatac aaagaaagtc tatcgtatca tgaaaaataa tggctggctt
     2821 tgtcggacac gtaccaagaa agttcccaat ttgggtaaag cttattattt aacagataat
     2881 aaattgagtc gggatttcca tgccgataag cctaaggaaa agctcgtaac agatattacc
     2941 tacctatatt tcggtaactg taaattgtat ctctcatcaa ttatggatct ctataaccga
     3001 gaaattatag cttacactat ctcagattgt caggacacgg attttgtact agacaccctt
     3061 aatcagttaa aattgcctaa ataaaggagc aatcttacac agtgaccaag gctcagtgta
     3121 tacttctatg gcttactacc aggcttgcac ggaaaaagac attatccgct caatgtctcg
     3181 aaaaggaaca cctgcagata atgcctgtat tgaatggttt cacaccgtcc taaagactga
     3241 aaccttttat ttccataata ggagaaaata caacaaagat agtataacaa atattgttaa
     3301 aaattacatt acattttata atgaaactag aattcaacag agattaaacg accagtcgcc
     3361 tgtacagtac agaaaactga ttgcctaaaa gtgtttttct tgcgtctcac tattagggtg
     3421 cagtgccaat atcatgcatt ctttttctaa tcctggatat ccttatgata atgccgtaac
     3481 tgaagcattt tcaagtattt aaagcataga caaatcaacc gaaaacatta tcaaaatatc
     3541 aaacaggttc aattagactg ctttgaatac attgagaatt tttataacaa ttacaaccca
     3601 catacggcta atctaggact aacccctaat cagaaagaag aaaattattt taactcaata
     3661 aaataacaca gttttctgtc tacttatttg acattagtcc aagaatcctc ctcttttttt
     3721 actattctaa catggatacc atttctttgc aaaagaaaac cgctattatc caacttttat
     3781 cactactcgt tacatagaat gacttcaaaa tcaccgtaac ttgctttgac aaagcagtgc
     3841 tttaacaatg agatcttctt ttggttaagg gtttatttgt tgaagtgtct tgatatcata
     3901 agttaccaat tcaccatttt tattataaaa ttggacacct tctggttgga aaatgagata
     3961 cttaggatcg atattagctt tttgtgctaa ttgattgata tgatcttcta gggttgctct
     4021 atctagacca tagtctggta aactgtctat aaagtcatct gattcttttt cttctttact
     4081 ggcttcactt ggctgttggt tttcattgct cttatctgcc tgttgctttt cttcaggttt
     4141 atccgtatca gtagcatcac cagcattttt ctttgccaat aactcttgag cttgttgcca
     4201 ctcagcttgg gatagatcgg atttattaat ggttctcaat gaactgccat ctgctcttgg
     4261 gatgctaaag gagccatctt tccatacaaa agtttctttt gccaaaacat ctcgtggatc
     4321 gaaaatatag ccgtctggtg ctgcaaaacg accttctgct agggcttttt gaacttcttc
     4381 agcagaatgg ataatttgcc agtttggcat accagcacgt ttatcaagag gcgtaacatt
     4441 tggaatgacc gaacctgact cttcatgccc tggcttagac catacatccg gacgaacttc
     4501 ggggtgttgc atcacatact tgattgttgc aatctgattg cgcgtcaacc atgaatacgg
     4561 aacgacatgg atatgatcaa tatgtgggat aacaaacgaa cttccagtat cgtaagtagc
     4621 attaccagca tgcatcggca gacttgacac atctacagca agtcgtggct caatgcctgt
     4681 atcaacaatg tcataacggt aatgcttctt atctttaagc attagttctt gttcggcaaa
     4741 ggcaatctga gtcaaatcaa gttgataacg agcatagaaa tagtccttgc catcttttgg
     4801 cataatatag cccactttac catcttttgt tactttgcga ctcacttttt tagtgtcaaa
     4861 gagtggtttt tctttgcctt gttcctgatc caaagcagca acaagctcat cagcttgacc
     4921 ttttgctttc acccagttag cgacctcatc caactcatat tgttcaagtt ctccaaatcc
     4981 tatatagtgg aaatgatctc cgtgtttagc tgtaactcct gatttatcca ctgaatgaat
     5041 ggattcttta ctaaaaacat aagcatcact cgtatcatat ggtttaccat ctaagccttt
     5101 tccgtaagct ttgatgcgat gaccaagaaa ggtatgtgtc acttctttat ctgatggttt
     5161 tgagtgatct gaacctgagt cgttgtcatc agtttggccg gctaagtatc gatctgctaa
     5221 ttccatttca agaggtgata actgacttct tgggataata tgataatgat ctccatgagg
     5281 caccacatac ccaaaagcgt ttgatttgat cacttgagtc ggttcaaaaa tcaacccatc
     5341 ttcttccaca tgacggtatt tcaaatcaag acggtgtagt tgatctaaaa gttccttaaa
     5401 ggtttttcct ttaaactcat ctctttggtg tttgttttgt gacgcatcat ttggcctagg
     5461 aggcgctgga tgataaccac cgttatctgg ctgatgacct tgtccagggt taggcgtcac
     5521 atcaggaatt ggggctttcc tacgacctgg ggctggtgtc gggcggtaat cagacggtct
     5581 agcacctcga ccttgttttt gactccagta ggcttgtgca gcagctagct cacttggaga
     5641 caaatctttt ttaggaatat aatgatagtg attaccatga ggtactaaat aagcatctcc
     5701 taaatcatca atgatatctg tcggactaaa aatatagcca tcgtctgtag tatagcgtcc
     5761 ttgtcttttt gcttcattga ctgccgcaac ttcttcttta ctgagatggg ccacttgagc
     5821 taaacctttt tctttagctt ctttagttcc tttggctact tgctcagcaa tttgttgttt
     5881 ggttcgaatg tttttgcgct tactacctgg cttgaggtaa acataatagt tgccattgac
     5941 tttaataacg taaccgtcta agatttcatt gataacgtct gattgtttaa aatggtaatt
     6001 aggatccgtc atcaacaact cttcactaat aatcgcatca taaggaactt tcccattgta
     6061 aaaatgataa tggtcaccgt gtgaggtaac ataaccttgg tcagtaattt tgactacgat
     6121 ctgttcagca gagatgcctt cttcagcact gatttgatcc atcgttttgt ttgttttagg
     6181 ggcttttacc ttacctttgc tatcatcaat ataggcaatc tgattgtcct ttgttgctag
     6241 acccatatga tgcttaccaa gctggtaact tccaatatga gtagctagta aaatagcagc
     6301 aactgagccg atataaccat atgttttctt cactaataat ctcctttact tcaactgttg
     6361 atagagcact tccaaatttg ctctaagatt ttctagatat gtcttgtttc cgcttggagc
     6421 agcttcaagt ggacttaatg tctttacttt agctcctgtt gatttcgcaa tagcatgagc
     6481 aattttgggg ttgacgttgt cttctgcaaa aatagtcttg acgttgtatt ctttaacaaa
     6541 gtcttgaatt tctttcaatt ggcgaggaga gggctcttgc tctggagaaa tacccgagat
     6601 accaagttgt ttcaagccga atcgtttagc cagataagaa aatgccgtgt gttgcgtcac
     6661 aaatgttttt gagcgcacct ttttaaattt ttgagtgtat tcttcagtta gttgctctgc
     6721 ttcttttttg aaagccttag cctttttagt gtaactgtct ttgtgtttag gatccaaatg
     6781 tcctagctct ttagcgatat taacagcttc ctcaccagct aaaacgggat ccgtccaggt
     6841 atgtgggtca taaagtgtcg cagggtcaat gccttgtgtg acttccatat cttctagccc
     6901 tttgactcta tctagtgtca gaggttttga cgcttcaaac acattaacct ttgatttttt
     6961 taaattaggg tctagatccc ttgcccaagc ttctaaggta tgtgaatggt aaacaaacaa
     7021 atccgcgtca taaatagctg ccacatcatt tacagacggt tcaaaggaat gaatgcctgc
     7081 acctgattgg atcatcctca catcatttag atctccagat acttcttttg tcatcgcata
     7141 cattgggtaa aagctggtta caactgacat gccttgcgta ggctgtttgg ggtttgctga
     7201 cttatcacac cctgctatca ttactaaact cacaaccata gccatgagaa aaaaaacttt
     7261 tttcatagta cctcctcaat tataatttaa ccagttatta accagttaat tttactcctt
     7321 tatcaatgct ttgtcaatga caactaccat cttttatcac atcaactgaa gggataagca
     7381 aaaggcaatt gttgacagct atttgtccta acttcttttt gatgaggtag ca
//
LOCUS       DATROY010000093         7347 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.094, whole genome shotgun sequence.
ACCESSION   DATROY010000093 DATROY010000000
VERSION     DATROY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..238)
                     /locus_tag="VBD11_001514"
     CDS             complement(<1..238)
                     /locus_tag="VBD11_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6954455.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGL"
     gene            477..830
                     /gene="yaaA"
                     /locus_tag="VBD11_001515"
     CDS             477..830
                     /gene="yaaA"
                     /locus_tag="VBD11_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6954456.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            833..1942
                     /gene="recF"
                     /locus_tag="VBD11_001516"
     CDS             833..1942
                     /gene="recF"
                     /locus_tag="VBD11_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6954457.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(1954..2820)
                     /locus_tag="VBD11_001517"
     CDS             complement(1954..2820)
                     /locus_tag="VBD11_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6954458.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(2876..3538)
                     /locus_tag="VBD11_001518"
     CDS             complement(2876..3538)
                     /locus_tag="VBD11_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954459.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVAKAIVFADLANKV
                     SQ"
     gene            complement(3614..5095)
                     /gene="guaB"
                     /locus_tag="VBD11_001519"
     CDS             complement(3614..5095)
                     /gene="guaB"
                     /locus_tag="VBD11_001519"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6954460.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(5252..5737)
                     /locus_tag="VBD11_001520"
     CDS             complement(5252..5737)
                     /locus_tag="VBD11_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6954461.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(5747..6427)
                     /locus_tag="VBD11_001521"
     CDS             complement(5747..6427)
                     /locus_tag="VBD11_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6954462.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            6635..7339
                     /locus_tag="VBD11_001522"
     CDS             6635..7339
                     /locus_tag="VBD11_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO6954463.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
BASE COUNT         2526 a         1367 c         1113 g         2341 t
ORIGIN      
        1 caagaccctt agtagaaatc ttagttgata aactagcacc atataaggaa gctaactttt
       61 ctcgaaattc ttgtacctta gggtacttag catttgcagt aactaacatt tgagcaacga
      121 gcgatctacg tgctactgtt ttattattaa aatcacctga aaatcgaaaa gtaagatgat
      181 tagtcttaaa ttgttggttt ttaattaaat gtagagaaac gccttcaact attttcattc
      241 aatatccttt gtgttctgta ttgttagcta agattatatc atttttaaag gcgttaaaaa
      301 agattaaaca tccaagatat gattaattct aaatttcaat cttcttcaaa attgacaaag
      361 tcgtcaacca atatttactc ccatttttta taccttgtct ccatatcata agttcaactc
      421 aaaaaataat tttatgctat aatgatgtga tatctaaaat tggagattaa aaagtcatgg
      481 attataaatt atttgatgaa tatattactt tacaatcact tctaaaagag attggtatca
      541 ttcaaagtgg cggagccatt aaaaaatttt tagcggataa tcaagtattg tttaacggtg
      601 accttgaaaa taggcgtgga aagaaactcc gcctggggga tattattact attcctgatc
      661 aaaatatcga gatcattata aggaagccat cagatcaaga aattgaagaa cgtaatgtag
      721 aaatagctga aaaacagcgt gtttcagcaa tcgttaaaga aatgaataaa aacaccaaca
      781 aagataaatc aaaaactagc aaaaaaccag ttcgctttcc agggacataa aaatgtggat
      841 aaaaaatatt tctttaaaac attatcgtaa ttatgaagaa gcacaagtag atttttcacc
      901 caacctcaat attttcattg gccgcaatgc tcaaggaaag acaaattttt tagaagcaat
      961 ctacttcttg gctttgactc gaagtcaccg tacccgttct gataaagaac ttgtacattt
     1021 taaacaccat gatgtacaaa ttacaggtga ggtaatccgc aaaagtggcc atcttaactt
     1081 agatattcaa ttatctgaaa aaggaagaat cactaaagtt aaccatctta aacaggcaaa
     1141 attatctgat tatattgggg ctatgactgt cgtacttttt gcccctgaag atcttcaatt
     1201 agttaaaggt gcacctagtc taagacgaaa atttttagat atagatattg gacaaattaa
     1261 gtcaacgtac ttagctgaac tttcaaatta caatcatgta ttaaaacaaa gaaacactta
     1321 tctaaaaact actaataacg tcgacaaaac ttttttaaca gtccttgatg aacaattagc
     1381 tgattatgga agtcgtgtca ttgaacaccg ttttgatttt atccaagccc taaatgatga
     1441 agcggacaaa catcactaca ttatcagtac tgagttagaa catttatcta ttcactacaa
     1501 atcgtcaata gagtttacag ataagtcaag tattagggaa cattttttaa accaattatc
     1561 taaaagccac tcaagagaca tctttaagaa aaacacaagc atcggacctc atcgtgatga
     1621 tattactttc tttattaatg atatcaatgc tacttttggt agccaagggc aacaaagaag
     1681 ccttatttta tcattgaaat tagcagaaat tgagctaata aaaaccgtta ccaacgatta
     1741 tccgattctc ttacttgacg acgtcatgag tgaacttgac aaccatcgtc aactaaaact
     1801 cttagaggga atcaaagaga atgttcaaac atttattact acaacaagtt tagaacattt
     1861 aagtgcttta cctgatcaac tcaaaatctt taacgttagt gatggtacta ttagtattaa
     1921 tgaaaaaaaa gcaacagatt aatctgttgc tttttaataa gatttaacaa taccaagtaa
     1981 tatagcaccc ataacaaaac aaaggatacc catgacaacc catttctgct ctttcttcgt
     2041 ttttgtctca cctaaaaata aaataccacc aataatagag ataattactc caagttgaga
     2101 aaaactaaaa gcaattgcta gccctgcttt agctgctgcc aataacatga agacattacc
     2161 aacgccccac atgagacctg taatcatatt tttaacaaca acagcctcaa agttaacacg
     2221 aaacttcatg aaacaaattg cacctagaca cattccaaca gccatgggta aaatgacggc
     2281 catagcgtcg aacttcataa tgttgttaaa taaaactgcg tacgagatgt aacctacagt
     2341 tgaataagca attgtagcaa atccttttga aaactcttga tgtgttgcca aagcttgttc
     2401 tgaaacatca cgtttacttg agaaatagaa gccgataact aataatgtca acgctgtcaa
     2461 tcctaaaata aattggattg gcttagtcca ttcatgaaag actaaagcac caactaggct
     2521 tccacctact aattgtgcac cacttgacag tggattagca acagagactc ccatatattt
     2581 cattgcttga aattgaccat tttggccgac tgaccatagg ataccaccta agataccaaa
     2641 aatccacaat gaggcagtca tctctggctg tttaaataac catacgataa tcgcaaatag
     2701 caatgctcct aaagtcattc caaatgtttg ttgatttgga cgccctccaa ttttattact
     2761 aacaaatcca atacttcccc acgcaaacat gggaatcaat gcaataagta aaccttccaa
     2821 ttctttctcc ttacaatgga atattaagta ttacctattt tcaatcctag agaatctatt
     2881 gactaacttt attagcaaga tcagcaaata ctatagcttt agcaacacta tctttttcca
     2941 aagctataat gacttgtttt atcaataaat tgaccagtga tttagaataa ggtaactgtt
     3001 ctttagtcat attactaatc ttatttaaaa taacatcttc aggtaaaata tcttgaaaat
     3061 agcagatgac tttgaaataa aaatcattgt actgtgtcac accattcaga taagtaacta
     3121 ccaggttaat ctcatcttga ggcagcctca tatttaaccc taaatttgaa ataaaaagtt
     3181 tagccaatat tttgagaaca ttgctttcta ttctatcaca ggattgttgt tcttggaaac
     3241 tgaaaattct catgatatct tcattatctc gataagattc cagtaaagtt aattgctcta
     3301 aatcagtatt agaagttcca gccttaactt ttgaatctag aatggatact tcattgatag
     3361 ttaccccaac ctccaacgtt tgtaaagctt taataaattt atcaatacct atttgtgtct
     3421 caccattttc aaaacgtgat agagttgatt ttgataattg agcacttttg gctagagaac
     3481 ttagggatat acctttgtct tctctcaact cttttaaaat ttttccataa tttttcatac
     3541 aaaaacttta ctatatttgt ttcttttatg caacaaaaag catcctcata ggggatgctt
     3601 ttttgtcctt attttagtgt actgaataat ttggtgcctc atttgtaatt tgaacatcat
     3661 ggggatggct ttctttcaaa ccagctcctg acatttcaac aaattgagca ttatcatgaa
     3721 gttcttttat gttcgctgca cctacatatc ccataccaga acgaatgcca ccaagcattt
     3781 ggaaaacaat atcggctact gaacctttgt atgctacacg accttcaata ccttctggaa
     3841 ccaatttgtt agcctcgttt acagaacctt ggaaataacg atcgcttgaa ccttttttca
     3901 ttgctgcaat tgagcccata ccacggtatg tcttgaactt acgcccttgg aaaatttctg
     3961 tttctcctgg cgcttcatct gttcctgcaa acattgaacc tagcataact gcatttccac
     4021 ctgctgcaag tgcttttaca atatcacctg aatatttaat accaccatca gcaatgatag
     4081 tcttaccgta ctcacgcgca acagccgctg catcgtaaat agctgtaatc tgaggaacac
     4141 ctaccccagc aacgacacga gtcgtacaaa ttgaacctgg accaatacca actttaacga
     4201 catccacacc tgcatcataa agtgcacgcg ctccttctgc tgtagcaata tttcccgcaa
     4261 tcaatgtgcg attagggaag tgagcacgaa tttcagcaat tttacggagc acaccagctg
     4321 aatgtccatg tgctgtatca atgacaatag catctgcacc tgcttcaaac aaagcttctg
     4381 cacgttcaaa agtatcagag gttacaccta ctgcacctgc aactaagaga cgtccaaatt
     4441 catcttttgc tgccttagga aattcaataa ctttttcgat atccttaata gtaataagtc
     4501 ctgaaagacg tccctcatca tctactaaag gtagcttttc aatacgatgc tcatgaagaa
     4561 tacgctccgc agtttcaaga tctgtaccaa taggagctgt tacaagattt tgacttgtca
     4621 tgtgctctga gattagttgt ttataatcag aaatgaaacg catatcacgg ttagtaataa
     4681 ttccaactaa tttacgattc tcgagtgtct ctacaatagg aactccacta atgcgatagt
     4741 tttgcatcaa ttcctcagct tcagagacag tattatctgg tgtcaagaag aatgggtcaa
     4801 taataacacc attttctgag cgtttaactt tacgaacttc ttctgcttga tcaactattg
     4861 acatattttt atgaatgata ccaagcccac cagcacgtgc aatagcaata gccatcttac
     4921 tatctgtaac tgtatccatt gcagctgtaa tgattggaat gtttaatgtc aaattatcag
     4981 ccaactttgt attcatatca acttcatttg gtaaaacatg gctttcagca ggaataagca
     5041 gaacatcatc aaaagtaaaa ccttttttca aaaacttagt atcccaattt gacatcaaaa
     5101 aatcctcttt tcttagatag tagatataaa aaatcccatt aaaattatat tgttactatc
     5161 atatcacgct aaaatcattt gtcaatataa aaagagcata gaattaatag cttctagctc
     5221 tttaaagtaa tgaaaaaatc ttcttaattt tctatttttt tgagaagaag aaaggcggcg
     5281 tatactgact taatttctca aaacactttt gagcttcttc actattatta caaataatca
     5341 aacaagtatc atcaccacag acagttgcta agatctcagg taataaaatc gaatctaata
     5401 tggagccaaa agattgagct aagccaggta gtgtcttcaa aacgatttgg ttttgaactg
     5461 gaaacagcat gactagggca tcttccatgt aaaaacgtaa ccgttcttcc caacgcttct
     5521 gagatatact atgaatctca taataagttt ctgtagcatt atcactgctt tcgttaacct
     5581 taattaaatt aagttccttc atatctcgag ataatgttgc ttgcgtcact gaaacgccat
     5641 ttttttctag taattccctt aactcatgtt gtgtacgcac cttagtctca gagactaagg
     5701 aacgaattaa ttggtgtctt gtttcttttt tattcatata aactccttat tcaatgaatt
     5761 tcttgaaata atttttatta ttatagagaa gcttctttcc tttcatttca acgatatctt
     5821 tttggcgtaa ttcctttaaa acatgactaa ctgtctcgcg agtagttccg ctcatattgg
     5881 caatttcaat cgttgtaata ggaaaaggta aatgtcccct ttctgtagga acttgcaaaa
     5941 gtagagttgc aagcgattgg ataactctca tactggcact tgaagtcacc atattacgaa
     6001 gtcgtatttc atgaactcgt aatagcttag aatatttccg acaaagatgc ttcatttgat
     6061 tgatattttg aagagagtat tcttcgtata aagccattgg cagagaaaaa tactcaatat
     6121 ccgtaatagc tactgctgaa aaatggtaat catcatccaa aaagagacct ccataaggaa
     6181 aaactgtatc ttgtctaaca taatctgtat aaataaaaga accagaagcg tctgattgtt
     6241 caatttttac atgtcctgac tggattaaaa acagtttttc acggtaatca ccttcaaaaa
     6301 ataaggtatg attttttaga gctgtacgat gtttaatgtg gctcacaata tggtcaaatt
     6361 gttcaatagt aaagtgttta aaatcatcta gttgtctgaa ataaaaatat tgttcttttg
     6421 taatcacctg attttctaga cctttctaat cattagatgc gaacattttt tgtataatga
     6481 aaatttttta atgtataaaa ttttcttgtc acgataaatc ctccttcatt atagcaaaaa
     6541 aattcgtaga aagaacgttt ttcgttgaat tttgaaaacg cttttgagaa aatgtaattg
     6601 ttctgatatc ttttaaaaaa attaagaggc cttcatgact aaatttattg ttgattcatc
     6661 gtactggaat ttgtttccaa cttctaaaat tggagtaatc ttaatcaaag attatcacat
     6721 ggacaggaac cttgaaacag aacttaaaca gttactgtct gatagtcatt ctttagcaaa
     6781 aaaatatttg caagagaaag aattcagtca aaacaaagtt atccaaactt atcgcaaagc
     6841 atatcaaacc tttaaaacta aaaaaggtgc acgttcaagt atcgaagcat tattgaagcg
     6901 aattaattca ggaaacgaaa ttactagtat aaatcctcta gttgatatct ataatgccgc
     6961 tagccttagg tttggattac ctattggtgc tgaggatagt gatacattta gaggtgattt
     7021 aaaactaact attaccaatg gtggtgatga attttacttg attggcgaag attttaacat
     7081 accaactctt tcaggtgagc ttgcctatgt tgatgatgtt ggagcagttt gccgctgttt
     7141 taattggcgt gatggaaaac gtactatgat taccgataat actcagaacg cttttttagt
     7201 tatcgaatta attgataata gaagagaaat tatttttaaa gaagcgttgg actttatcgc
     7261 caccaataca aatcgctttc taaaagctaa aacacagacc atcatattag ataaagaaca
     7321 cagtgaaata acgctataac attaaaa
//
LOCUS       DATROY010000094         7235 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.095, whole genome shotgun sequence.
ACCESSION   DATROY010000094 DATROY010000000
VERSION     DATROY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            6..2639
                     /locus_tag="VBD11_001523"
     CDS             6..2639
                     /locus_tag="VBD11_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6954464.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            2739..3398
                     /locus_tag="VBD11_001524"
     CDS             2739..3398
                     /locus_tag="VBD11_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6954465.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            3407..3871
                     /locus_tag="VBD11_001525"
     CDS             3407..3871
                     /locus_tag="VBD11_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954466.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            3871..4308
                     /gene="msrB"
                     /locus_tag="VBD11_001526"
     CDS             3871..4308
                     /gene="msrB"
                     /locus_tag="VBD11_001526"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6954467.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            4457..>7235
                     /locus_tag="VBD11_001527"
     CDS             4457..>7235
                     /locus_tag="VBD11_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO6954468.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDNNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIMI
                     GLDEVRKLFSSR"
BASE COUNT         2496 a         1155 c         1398 g         2186 t
ORIGIN      
        1 gactcatgaa aggtcaaaaa attattgctc tagctggtct tgtcctgtca tgccattttg
       61 ctcttacggc atgtcatact caagaacaca aaaattctta tcatattaaa acaaagcagg
      121 ttgctaagaa aaaagctaat aaaaagaaag tctctgttaa ggaaagtcat aaaaaacgca
      181 agggtgttgc aggggttgac tttccaactg atgatggctt tttactgact aaagattcga
      241 agattttatc tcatacagat tctggaatcg ttgtagcaca tggaaatcat tctcatttta
      301 ttttttactc tgatttaaaa ggttcaaaat ttagctattt aatccctaat gctaatgcaa
      361 aaactaataa aaatcaagcg gtaagaaact ttaaagcagg ggcggtagct gttaatacat
      421 taaatgatgg ctatgttttt aatcccgcag atattgtttc agaagatgct aatggctacg
      481 tggtcagaca tggtgaccat ttccactata ttccaaaagc aagtttatct cagcaaaagc
      541 aagtacaagc aagtagagct gtttcacgtt taggaaatca aagtaatagc cattacaggg
      601 ttaatagttc taaaattgca gggcttcact acccaacaag tgatggtttc ttatttaatg
      661 gacgaggaat aaaaggcact accccaacag gtattttagt agaacatcat aatcatctgc
      721 attttattag ttttgctgat ttaagaaaag gggggtgggg atcaattgct gaccgttacc
      781 aaccacaaaa gaaagctgat tctaaaaaac agtcaccatc aagtaaaaag ccaagaaatg
      841 aaaatacttt acctaaggac atcaaagata agctagctta ccttgctcgt gaattacatt
      901 tagatatttc acgtatcaga gtccttaaaa cactaaatgg tgaaattgga tttgaatacc
      961 cacatgatga tcatacacat gttattatgg ccaaagatat tgatctaagt aagccaattc
     1021 caaatccaca tcatgatgac gaagatcatc acaaaggtca tcaccatgat gagtccgacc
     1081 ataagcatga agaacacgag cacactaaat caaataaatt atctgatgaa aatcaaaaga
     1141 aattaattta tttagcagag aagcttggtt taaatcctaa tcaaattgaa gtattaacgt
     1201 cagaggatgg aagtatcatc tttaagtatc cacatgatga ccactcacac actatagcaa
     1261 gtaaggatat cgaaattggg aagcctatcc cagatggaca ccacgatcac tctcatgcaa
     1321 aagataaagt tggtatggca actctaaaac aaattggttt tgatgatgag attattcagg
     1381 atattctgca tgcagatgct ccaacaccat tcccatcaaa tgaaactaat cctgaaaaaa
     1441 tgcgtcagtg gcttgcaact gttactaaga ttaacattgg acaaagaacc aatccattcc
     1501 agcgttttgg tctatcattg atgcctaata ttgaagttct aggaattggg tttactccga
     1561 ttaatgatat gacgccagtt ttgcaattca aaaaacttaa acagctttgg atgacaaata
     1621 ctggtattac agattattct ttccttgata agatgccact attagaagga ttggatattt
     1681 cacaaaacgg tattaaggat ttatctttcc ttacaaaata taaacaatta agtcttattg
     1741 ctgcagctaa taatggcatt acttcgctaa aaccattggc tgaattacca aaccttcaat
     1801 tcttagtttt gagtcataac aatatctctg acttgacacc gttatcaaac ttgacaaaac
     1861 tccaagaatt gcaccttgat cataataatg tgaagaattt aagtgcactt tctggtaaaa
     1921 aagatttgaa agttctagat ttatcaaaca ataaatccgc agacttatcg actctaaaaa
     1981 caactagtct tgagacgctt cttttaaatg agacgaatac aagcaatcta agtttcttaa
     2041 agcaaaatcc taaggtatct aatttaacaa ttaataatgc gaaattatca tcattagatg
     2101 gtattgaaga gagcgatgaa attgttaaag tagaagctga agggaaccaa attaagtcac
     2161 tagtattgaa aaataaacaa ggttcgttaa aattcttgaa tgtgactaat aatcagttaa
     2221 catcacttga aggtgttaat aattatactt cacttgagac cttaagtgtt tctagaaata
     2281 aacttaaatc tttagatatt aaaacaccta acaaaacagt tacaaatctt gattttagcc
     2341 ataacaatgt tccaacatca caattgaaat tgaatgaaaa aaatattcct gaagcagtag
     2401 ctaagaattt tccagcagtt gtagaaggtt caatggttgg aaatggtagt cttgctgaaa
     2461 aagcagctat ggcttctaaa gaggacaaac aagtttcaga taatactaat caccaaaaaa
     2521 acactgaaaa atctgctcaa gcgaatgctg atagtaaaaa agaaaaccct aaaacacatg
     2581 atgaacatca tgaccatgaa gaaacagatc atgcacatgt aggtcatcat caccattaat
     2641 cttttacgga gctaatatat aaaatccttc aagaatttct tggaggattt tgcatttata
     2701 tgctaaatca tttatttttt tagaaaatta gctataatat gactatggtt aaaagagaag
     2761 atgtgatttt aaatgcaact gaaaatatgg tttaccataa attaaaaaat gatccaagtg
     2821 gccatgactg gtttcatatt gttagagtaa gaaatcttgc agtagaatta gcacacaaag
     2881 aaggtgctaa tacctttatt tgtcaaatgg ctgcactttt gcatgatatt attgatgaca
     2941 aaatctgcca agatagtaag caagcgtcgt atgaattgac tcaatggtta tatagtcaag
     3001 atcttgccat agcagaagta gaacatatcc ttgatattct tgaaaatatt tcattcaaag
     3061 caggaactgg attgactatg aaaacattgg aaggtcaaat tgttcaagat gcagatcgcc
     3121 tagatgctat gggggctatt ggtattgcaa gaacgatggc ttattcaggg agtaaaggac
     3181 gtttaattca tgaccctaac ttaaaaccac gagaaaacct cactttagaa gagtatcgta
     3241 atggtcagga tacagctatc atgcattttt acgaaaaatt attaaagtta aaagacctta
     3301 tgaatacaaa acaagggaaa atgcttgctc aaaaacgtca tgatttttta gaactatatt
     3361 tagccgaatt ttatgctgaa tggaatggta aaggttagga gtagatatga ttcgtagagc
     3421 gaaagaaaaa gatttgcctg ctattgcaga gctattgaaa caaattttag tgttacatca
     3481 tggggttcgc ccagatattt ttcatacaag aggaagtaag ttttctaaag aacaattgaa
     3541 agaaatgctg atggacgaat caaggcctat ctttgtttat gaatcagacg agggtaaagt
     3601 tgttgcccac ttgtttcttc aattacaaga aaaacgtgac ttacctcgaa agtcctttaa
     3661 aactctctat atagatgatt tgtgcattga tgaggaagta agaggtcagc aaattggaca
     3721 aaagttgatg gattttgcaa gacaatatgc taagaaacat gggtgttata atataacttt
     3781 aaatgtttgg aatgataatc agagagcggt ctctttttat gaaaaattag gttttaaacc
     3841 tcaacaaact caaatggagg agatactata atgaaagaaa cacaagaaga attacgtcag
     3901 cgtatcggac atacagctta tcaagtaacc caaaattcag ctacggaaca tgcttttact
     3961 gggaagtatg atgatttttt tgaagagggg atatatgtag atatcgtcag tggagaggtt
     4021 ttattctctt ctttagataa atttcaatct ggttgtggtt ggccagcatt ttcaaaacca
     4081 attgagaatc gaatggtgac aaatcatcaa gatcactctc atggaatgca tcgtattgag
     4141 gttaggagtc gtcaagctga ctctcattta gggcatgtat ttaatgatgg accggttgat
     4201 gcaggtggct tacgctattg tatcaattca gctgccttag attttattcc ttatgaccag
     4261 atggcaaaga gaggctatgg ggactactta tcactttttg atgaataaga gacgacttaa
     4321 gggtatatct taagtcgttt tttatgtatg gtaaaaagaa ttggtggcag gtgtattctt
     4381 catcagttta ttttctgaaa aaagagtata atataggtat caaaaatgaa aacgttttag
     4441 agaaggagac tagaatatgg cttctacaaa taatgtttca gatacaaaca cacgttttgc
     4501 taaagaggaa ttagaagagg ttttcgaaga attagggaca actcaaggtg gtttatcaga
     4561 tgaagaggtt gctgtacgtc agaaaaaata tggtttaaac ctgttatctg aagtaaagca
     4621 agaatcaatt attttattgt ttctgaaaaa ttttactagt cttatggcaa tactattatg
     4681 ggttggagga tttgtggcta ttgtttctaa tagcttagag ctaggtcttg ccatttggat
     4741 ggttaatgtg attaatggta tttttagttt cattcaggaa taccgtgcaa gccaagcaac
     4801 acaagctctc aagaaaatgt taccatctta ttcacgtgtt cttagaaaaa gctcagaaga
     4861 aaaggtatta tcagagcaac tagtcccagg agatattgtt cttattgaag aaggagattg
     4921 tatttcagca gatggtcgct tgattaaaac aacagatttg caagtaaacc aatcagcttt
     4981 gacaggggaa tcaaatccta tttataaaga taacaatgtt gaaaactacc agtctaaaac
     5041 attgattgaa tgtgataata tggttttcgc aggaacgaca gtctccagtg gtagcgcaac
     5101 aatggttgtt acggcaattg gaatgcaaac ccaatttggt caaatcgctg atttaacaca
     5161 agggatgaaa tcagaaaaaa gccctcttca aagagaactt gatcggttga ccaaacagat
     5221 ttctatcatt tcaattactg taggaattat ctttttctta gcagcgactt tttttgttaa
     5281 ggagccagtg tcaaaatcat ttatctttgc cttggggatg atcgttgcat ttatcccaga
     5341 aggtttacta cctaccgtta cactttctct agctatggca gtccaacgaa tggctaaaga
     5401 acacgcactt gtcaaaaaac tttcttcagt tgagactctt ggagcaacct cagttatctg
     5461 ttctgataag actggtacat taacgcaaaa tgaaatgacc gtcaatcatt tgtggcaaaa
     5521 tggaaagagt taccaagtga caggactggg ttatgctcca gaaggacaga ttctttttga
     5581 aggtgacaat atttgctttg gtaatagtga tagaggagac ttagaaaaac ttattcgttt
     5641 tgctcatctc tgtagcaacg ctcaagtatt accaccaaat gatgatcgtt caacttatac
     5701 tgtacttgga gatccgacag aagcttgttt gaatgtttta ttggaaaaat cagggataaa
     5761 catacaagaa aatcgtaagt ttgcaccacg tttgaaagag ttaccttttg attcggttag
     5821 aaaacgcatg acaactattc atagcttagg aggcgatgaa aaggataaaa aaatttcaat
     5881 cacaaaaggt gcgccgaaag aaatacttga tttatcagat tatgtcttat ctgatggcaa
     5941 agttatcccc ttaaataagg aagaacgtaa taaaatacag ttggctaatg atacatttgc
     6001 taaagatgga cttcgcgtct tagctgtttc atactgcgat attgaaggct tttcaaaaga
     6061 acaatggacg caagaaaatt tggaacaaca catggtgttt atagggttga tagcaatgag
     6121 tgatccacct cgtgaaggcg ttcgtgaagc tattgataaa tgtcatgcag caagcattcg
     6181 tattattatg gtgactggag attacggatt aacggcactg agtatcgcta aaaatattgg
     6241 tattattcgt aacgatgatg ctaaagtaat ttcaggttta gagttatcag aaatgacgga
     6301 tagccaattg aaaaaagaat tgtcaggtga agtcgttttt gctcgtgtag caccagaaca
     6361 aaaatatcga gtagtaacga tattacagga gatgggtgaa gtagtagcag ttacaggaga
     6421 cggggtcaat gatgcgcctg cgctaaaaaa atccgatatt ggtgtagcca tgggtgttac
     6481 cggtactgat gttgctaaag aatcagctga tatgattctg acagatgatc attttgcttc
     6541 tattgtacat gccgtggaag aagggcgggc tgtttatcaa aacattaaga aattcttgac
     6601 gtatattttt aatagtaata ctcctgaggc ggttccgtcg gcattcttcc ttttttctaa
     6661 aggttttatt cctttaccat tgactgtaat gcaaatactt gctgttgatt tgggaaccga
     6721 tatgcttccc gctttaggtc taggcgtgga accaccagaa acagatgtta tgaatcgtcc
     6781 tccaagacgg ctaacagatc gcttattgga caaagggcta cttataaaat ctttcctttg
     6841 gtacggcact attgaatccg tattagctat gggtggtttt ttctgggctc attacttacg
     6901 ttatggtaat tttacttttt tcgtcgcaaa cggcataccg tacagagaag cgacaactat
     6961 gacacttgga gctattatct ttagccagat aggtatggtt atgaatagca gaacgagcta
     7021 tcaatctatt aaaacactat ctatttttgg taataaatta attaattttg gtataataat
     7081 ggagatttta gcatttttag tcttagtata cgtgccacta ttccataact tattcaacac
     7141 agcaagccta ggtttatcac attggctcta tttaataagc tgcccgttta tcatgatagg
     7201 tttagatgaa gttcggaaat tattcagcag tagaa
//
LOCUS       DATROY010000095         7193 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.096, whole genome shotgun sequence.
ACCESSION   DATROY010000095 DATROY010000000
VERSION     DATROY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            109..1341
                     /gene="arcA"
                     /locus_tag="VBD11_001528"
     CDS             109..1341
                     /gene="arcA"
                     /locus_tag="VBD11_001528"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6954469.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            1347..1777
                     /locus_tag="VBD11_001529"
                     /pseudo
     CDS             1347..1777
                     /locus_tag="VBD11_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1793..2806
                     /gene="argF"
                     /locus_tag="VBD11_001530"
     CDS             1793..2806
                     /gene="argF"
                     /locus_tag="VBD11_001530"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6954470.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            2869..4296
                     /gene="arcD"
                     /locus_tag="VBD11_001531"
     CDS             2869..4296
                     /gene="arcD"
                     /locus_tag="VBD11_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6954471.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            4317..5273
                     /gene="arcC"
                     /locus_tag="VBD11_001532"
     CDS             4317..5273
                     /gene="arcC"
                     /locus_tag="VBD11_001532"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6954472.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
     gene            complement(5393..6418)
                     /gene="trpS"
                     /locus_tag="VBD11_001533"
     CDS             complement(5393..6418)
                     /gene="trpS"
                     /locus_tag="VBD11_001533"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6954473.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(6526..>7193)
                     /locus_tag="VBD11_001534"
     CDS             complement(6526..>7193)
                     /locus_tag="VBD11_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6954474.1"
                     /translation="QDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGNIILMVANLG
                     QNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTMFIPFVENVN
                     HNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYIVNRQRTAMQ
                     KRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTIEIIKNDLTL
                     HNV"
BASE COUNT         2359 a         1283 c         1287 g         2264 t
ORIGIN      
        1 atgcgaataa cttccctatt ttatattcaa aagaaagtag aatgtaagtg tagatataga
       61 gataaatcct gtttcaatgc aaatctctaa ctaagaaagg agtaaaatat gactcaaaca
      121 catccaattc atgttttctc agaaattgga aaacttaaaa aagttatgct acaccgccca
      181 ggtaaagaaa tagaaaatct gatgcctgat tatttagaga gactcttgtt tgatgatatc
      241 ccatttttgg aagatgcaca aaaagaacac gatgcgtttg cacaagcact tcgcaacgaa
      301 ggagtcgaag tactttacct tgaaaatctt gctgccgagt cgctaacaaa ccaagaaatt
      361 cgtgaacagt ttatcgatga gtacattggc gaagcaaatg ttcgcggtcg tgctactaaa
      421 aaagctattc gagaattatt actcaatatc aaagataata aagaacttat cgaaaaaact
      481 atggcaggta tccaaaagtc tgagctacct gaaatcccat cttctgaaaa aggtctaact
      541 gatcttgtcg aatctaatta tccatttgcc atagatccga tgccaaatct ttactttaca
      601 cgtgatccat ttgcaactat tgggaatgga gtttcattaa accatatgtt ctctgaaaca
      661 cgtaatcgcg aaactttata cggtaaatat atctttacac atcacccaga gtacggtgga
      721 aaagtcccta tggtctatga tcgtgaagaa acaactcgta ttgaaggtgg agatgagctc
      781 gtcctttcta aagatgtcct tgccgtgggt atctctcaac gtactgatgc tgcctctatt
      841 gaaaaactct tagtcaatat ttttaaacaa aatttaggtt ttaaaaaggt tttagcattt
      901 gaatttgcaa ataatcgtaa atttatgcac cttgacacag tctttacaat ggttgactat
      961 gacaagttta caattcaccc agaaattgaa ggcgatcttc gcgtttactc tgtaacttac
     1021 gaaaaccaag atcttcatat tgaagaagaa aaaggtgatt tggcagatct tctagcgaaa
     1081 aacctaggag ttgagaaagt ggaattgatt cgttgcgggg gtgacaactt agtcgctgct
     1141 ggacgtgaac aatggaatga tggttcaaat acattaacta tagcaccagg tgtcgttgtc
     1201 gtttacaaca gaaatacaat cacaaatgca atcttagagt ccaaaggatt gaaattaatt
     1261 aagattaatg gaagtgaatt agttcgtggg cgtggtggac cacgctgcat gtcaatgcca
     1321 tttgaacgtg aagaccttta aaatttatga agctattagt caatcacttg ccttaccaaa
     1381 gagctgcaag cctatatatt cgcttttctg ttttcgttat cgaaagaaat ataaaaatga
     1441 aagaagaatt cgatgataat gacgagcaag atactattta tgctgttctt tatgatggaa
     1501 acagccagtt tcaacaggac gctttcttcc tgaaactcaa acagaagctc gtttgactcg
     1561 aattgcgact ttaaaaggtt atcgtggtaa cggctatggt accaaaatta ttatagcact
     1621 agaaaattat gcaaaagaaa atggttatca ctatctaact atacatgctg aattgacagc
     1681 aaaagatttc taccagacac ttgggtatca agctactggg aatatttata tggaagacgg
     1741 ggaagcctgt caaacactcg aaaaatattt aatttaaagg agacattaac atatgacaca
     1801 agtatttcaa ggacgtagtt tcttagcaga aaaagatttt tctcgtgagg aatttgaata
     1861 tcttattgat ttttcagctc atttaaaaga ccttaaaaaa cgtggtgttc ctcatcatta
     1921 tcttgaaggt aaaaatattg ctctcttatt tgaaaaaaca tctactcgta ctcgcgcagc
     1981 ctttacaact gcagcaattg acctaggcgc tcatccggaa taccttggtg caaatgatat
     2041 tcaacttggt aaaaaagaat caacagaaga tactgctaag gttttaggac gtatgtttga
     2101 tggtattgaa ttccgtggtt ttagccaaag aatggttgaa gagcttgctg aattttctgg
     2161 agtacctgtc tggaatggtt taacagatga atggcatcca acacaaatgc tagctgacta
     2221 ccttactatc aaagaaaact tcggtaaact tgaaggtatt actcttgttt actgtggtga
     2281 cggacgtaac aatgttgcca actcgctttt agtggctggg actttgatgg gggtcaatgt
     2341 acacatcttt tctccaaaag aacttttccc tgctgaagag attgttaaat tggctgaagg
     2401 atatgccaaa gaatctgggg ctcacgttct cgttactgat aatgtagacg aagctgtaaa
     2461 gggagcagac gtcttttaca ctgatgtctg ggtatcgatg ggagaagaag ataagttcaa
     2521 agaacgcgtt gaacttcttc aaccatatca agtaaacatg gaactgatta aaaaagctaa
     2581 taatgataat cttatcttct tacactgctt acctgcattc catgatacaa ataccgttta
     2641 tggcaaagac gtcgctgaaa aatttggggt caaggaaatg gaagttactg atgaagtctt
     2701 ccgtagcaaa tatgctcgtc atttcgacca agctgaaaat cgtatgcaca ctattaaagc
     2761 tgtaatggct gcaacccttg gaaatctttt cattccaaaa gtttaaatta aacgactata
     2821 gggagttgac atatagtcaa ttccctattt taggaaatga ggtataaaat ggaaaaagaa
     2881 aaaaaattag gtcttttacc actaacaatg cttgtcattg gctctcttat cggtggcgga
     2941 atctttgatt taatgcaaaa tatgagttcc agagccggtt tggtaccaat gcttattgct
     3001 tgggtaatta ctgctatcgg gatgggaact ttcgttttaa gttttcaaaa tttatctgaa
     3061 aaaaggccgg acctaacagc tggaatcttt agttacgcta aagaggggtt tggaaacttt
     3121 atgggattta actctgcatg gggttattgg ttatcagctt ggcttggaaa tgttgcctac
     3181 gctgcactct tattcagttc actcggttat ttctttaaat tctttggtaa tggaaataat
     3241 atcatctcaa ttattggagc aagtatagtt atttgggttg tccatttctt aattttaaga
     3301 ggtgttaata cagctgcatt tattaatacc gtagttacct ttgcaaaatt agtacctgtt
     3361 attattttct taatttcagc gttattagct ttcaaattta acatttttag tcttgatatc
     3421 tggggaaatg gattacatca atcaattttc aaccaagtca attcaactat gaaaaccgct
     3481 gtttgggtat ttattggtat tgagggcgcc gttgtcttct caggtcgtgc taaaaaacac
     3541 tctgatattg gtaaagcaag tatcctagca ttattcacta tgatttcact ttatgtattg
     3601 atttctgttt tatcacttgg tatcatgtca cgtccagaac ttgcaaactt aaaaacacca
     3661 gctatggctt acgttctaga aaaagctgtt ggtcactggg gtgctatctt agttaacctt
     3721 ggtgttatca tttcagtatt tggcgctatt cttgcttgga ctttatttgc agcagaatta
     3781 ccatatcaag ctgctaaaga aggtgctttt cctaaatttt ttgcaaaaga aaataaaaac
     3841 aaagctccaa tcaactcact cttagtcact aatctttgtg tacaagcatt cttaatcacg
     3901 ttcttattca cacaaagtgc ttatcgtttt ggtttcgcat tagcatcatc tgctatctta
     3961 attccttatg cttttacagc actatatcaa ttacaattca cactccgtga ggataagtca
     4021 actccaggac atcaaaagaa tttaattatc ggtatcctcg ctacaatcta tgctgtttac
     4081 cttatctacg ctggtggttt tgattactta cttttgacaa tgattgctta tactctaggt
     4141 atgattctct atattaaaat gagaaaagat gacaagcttc ctatctttgt gagatatgaa
     4201 aaaattagtg ccattgtcat tctagcgctt tgcttactat gtattattga aattatgact
     4261 ggacaaattg acattgctgc aatcctagca ggttagtcat tagaaagagg gataaattgg
     4321 taaaagaaca tcaaaaaatc gtagttgcct taggtggtaa cgctattcta tcaacagatg
     4381 cttcagcaaa agcacaacaa gaagcattaa tcaacacatc aaaatcactg gtcaaactga
     4441 ttaaagaagg ccatgatgtc attgttactc atggaaatgg tccccaagtt ggtaacttat
     4501 tgctacagca agcagattca gattctgaaa agaatccagc aatgcctttg gatacttgtg
     4561 ttgccatgac agaaggaagt attggttttt ggttacaaaa tgctcttaac aatgaattac
     4621 aagaacaagg cattgacaaa gaggttgcaa cagtggtaac tcaagttatt gtagatgaaa
     4681 aagatcaagc tttcactaat ccaacaaaac ctattggccc attcttatct gaagaagatg
     4741 ccaagaaaca agcccaagaa acaggttcaa aatttaaaga agatgctggt cgtggatggc
     4801 gtaaagtcgt tccttcacca aaaccggttg gtatcaaaga agcttctgtt attagacggt
     4861 tagtggactc tggtgtggtt gttattagcg caggtggcgg aggtgtacca gttattgaag
     4921 atgctaatac aaaagcatta aaaggggttg aagctgttat tgacaaagac tttgccagtc
     4981 aaactttatc agaacttgtt gatgccgact tattcatcgt cttaactggc gttgacaacg
     5041 tttttgttaa ttttaataaa cctaatcaag aaaaattaga ggaagttact gtttcacaaa
     5101 tgaaacaata tatcactgaa aatcaatttg cccctggcag tatgctacca aaggtagaag
     5161 ctgcgattgc ttttgttgaa aataaaccag aagcgcgtgc tatcattact tcacttgaaa
     5221 atattgataa tgtcttagca gaaaatgcag ggacacaaat tgttgtaaat taaacaatgt
     5281 agattaccat tacatacact ccttattaat aacaaagtaa tgtcactcct taggcattcc
     5341 tataataaaa gagagctaga tatatctagc tctcttttat tataggaata tattatttaa
     5401 aatagttcaa acccatggct gatttaacct catccaaagt agaggctgca acagcttgag
     5461 ctttctcact acctttttgt aacatttgat aaacttgtcc catatcttta gcatattcaa
     5521 ggcgacgttc tctaataggt gataattcac gctctaagat atcaagtaga taacgttttg
     5581 ttttaacatc tccaagtcca cctttttgat agtgttcttt catagcagta atctcttttt
     5641 ggtcttcatc tcttccaaaa acatcaaggt aatggaatac catattacct tcgatttgtc
     5701 ctggttcttc tactttaata tggtttggat cagtatacat gctcataact tttttcttaa
     5761 ctgtatccat atcatctgct aaaaagattc cattatttaa tgacttagac atttttgcat
     5821 taccatctaa ccctggcaga cgacctgctg catcattttc tggatagatt ccctctggtt
     5881 caactaaaac ctgacaatta taagcatgat tgaaagatct aactatttca cgtgtttgtt
     5941 caatcattgg tttttggtca gtccctacag gaacaaggtt agctttaaaa gcggttatat
     6001 cagctgcttg tgcaacagga taaactaaga atcctgctgg aatagactca ccaaaacctt
     6061 tttgagcaat ttctgttttt acagttggat tacgttccaa acgtgcaagt gaaaccaagt
     6121 tcatataata catacttaat tctgctaact caggaatttg actttgaatg aataaagtcg
     6181 acttgtttgg atccagccca acagctaaat aatcaagtgc aacattacca attgattcaa
     6241 caatagtttg cggatcttta gcatggtctg tcagtgcttg ttggtctgct aaaaagacaa
     6301 ataaagtata gctaccctca ttttgaagta gaactctatt ttttaagcta cctacataat
     6361 gtccaatatg caactttcct gtcggtctat cacctgttaa gataattggt tttgtcatgt
     6421 ttactcctta atataccaaa gatatcattt catatagtta tcttatcatg tcacaagccc
     6481 tattgcaatt catccctaca agaaacattc accttattaa gatgattaaa cattatgtaa
     6541 cgttaaatcg ttcttaataa tttcaatcgt ataatgaatc ataataatta aaagcgttat
     6601 cgtcacacct aaaatagctc tatatgcaaa gatatcgtaa actattgcag ctaccaatgc
     6661 tccaataata aatgctagaa ctaccagtga ataattgata gctcgtcttt tttgcatagc
     6721 cgttctctga cgattaacaa tatactcata ccaaaattgt agcattttcc gataattacc
     6781 agaagtcatt aagatagtgt atggttcaga ttcaatgcga cttcctgaaa atgtcaacat
     6841 taaaatacct gtgctaaagg ctaataaagc tacccagata ttgtggttta cattttcaac
     6901 aaaaggaata aacatcgtag aaagtaaaag gggacataat gcatatagac gccaaaaagc
     6961 tgttactgca cgttctttaa tcacaatacc aaccaaaaaa ccaattgaaa aaaagaaaat
     7021 agaaaataat ttagtgataa aaaagtgaaa atcattttgt ccgagatttg ccaccatcaa
     7081 gataatatta cctgtctgtg ttgcaacaag ggagtgaaac tgcatgtgac tataaacgtc
     7141 aataaaacca ccaataaaac ccaataatac agccattttt cttccatctt gtg
//
LOCUS       DATROY010000096         7155 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.097, whole genome shotgun sequence.
ACCESSION   DATROY010000096 DATROY010000000
VERSION     DATROY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(71..769)
                     /locus_tag="VBD11_001535"
     CDS             complement(71..769)
                     /locus_tag="VBD11_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954475.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(770..1138)
                     /locus_tag="VBD11_001536"
     CDS             complement(770..1138)
                     /locus_tag="VBD11_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6954476.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            1283..4387
                     /locus_tag="VBD11_001537"
     CDS             1283..4387
                     /locus_tag="VBD11_001537"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6954477.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKKRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            4468..5490
                     /gene="pfkA"
                     /locus_tag="VBD11_001538"
     CDS             4468..5490
                     /gene="pfkA"
                     /locus_tag="VBD11_001538"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6954478.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            5539..7041
                     /gene="pyk"
                     /locus_tag="VBD11_001539"
     CDS             5539..7041
                     /gene="pyk"
                     /locus_tag="VBD11_001539"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6954479.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         2255 a         1145 c         1422 g         2333 t
ORIGIN      
        1 agtgttagat accacttacc aactgatttt aattcatatt ttaataattt tccaaacata
       61 tcaatctcct ttatttcttg aatctttcac gaaagagagc atcaatcgaa cagttgtgct
      121 cttctcttaa gtcatcagca ttgccttgta ggtcaatttc tccttcttta aggaagataa
      181 cttcatcaag gataggttca atgtcagaaa ttaagtgagt cgaaattaaa acagaagcat
      241 cattagagta gttgctaatg attgttttta aaatgtagtc acgcgctgca gggtctacac
      301 caccaatagg ttcatcaagg atgtagagcc tagctttacg actcataaca agtattagtt
      361 gaactttctc cttgttccct tttgataggt tttttagacg ttccttagga tttaggttaa
      421 gatcctcaaa tagttgataa gctacctttg aattaaaatc gttatagaaa tcttcaaata
      481 atgtaacgac gtctttgatt ttcatatttt cttgtaaata tgaagtatca gggagatatg
      541 aaataatttt tttggtttca acagacggtc tgtaaccatc gataacaata tctcccttat
      601 ctggctgaag taatccattt atcattttga tgattgttgt ttttccgcta ccatttggac
      661 ctaataaacc tataatcttt cctgttggaa tgcttacagt cacatcatta acagcaatat
      721 gtttgtgata tttctttgta acatgatgaa gttgtaataa ctgtgtcatt taattaatct
      781 cctttataaa atgatcgaga gcaaccgtaa tttcatctaa tttatatcct atttttttca
      841 tgttgttgac aaaagttgct aactctgctt ttgcaacttg ttgacgaatt ttaccaatta
      901 gtaagttgtc ttctgtaaca aagcgtccgg aagtacgttg tgagaatacc attccttcac
      961 gttccaattc agtgaaggca cgttgcatgg tatttgggtt aacaccagct tcttgagcta
     1021 actctctcac ggtaggcaat tggtcaccac tttttatttc ttgactaaca atttgcatct
     1081 ttatatgttc agcaatttgt gaataaatag gtgatttttc attgaattcc caagccatag
     1141 ataactcctt tcataatctt gtactttttg tactataaaa atagtacaaa aaaatatttg
     1201 aaatgtcaag gaattatcat tgagacttta atcgtttatt gtttcatagt ataatagtaa
     1261 aaaatcgtaa aaaggggcta ctatgtttgc acaacttgat acaagaaccg tttattcttt
     1321 tatggatagc ttggttgatt taaagacata tgtctctaaa tcgaaatcat taggatatca
     1381 aactattggt atcttggacc acagtaatct ttatgcagca tatcatttta ttcaagaggc
     1441 gcaaaaggca aacctaagac caatagttgg atttagcttt gatatagtgg ttgaaaatag
     1501 atcaatagaa gtttactgta ttgcgataaa tacagttggt tataagaatc tcttgaagtt
     1561 gtcaacggct caaatgtctg agaagatgtc tctaaattta ttgacagaac atcttgaagg
     1621 ggtgcaattg attcttccct atcaagatgc tcttgatcag ctcaatttac cctttgatta
     1681 tgttattggt gttaatttga cctctccgca gattccatat accaaaccta ttatagccat
     1741 agatactgtt cgatattttc aaaaaaatga tattgagaca ttacagatgc ttcatgctat
     1801 aagagataat gtcctgttaa aagatgcaca atatgcgagc aaaaatcaag aattaaaacc
     1861 ttgtcaagaa atgactctag cattcaagaa acgttttcct gaagctttag ctaacctaga
     1921 atctttggta gagaatgttg tttatcactt tgattctgac tttaagttac ctatttttaa
     1981 tagagaaatt cctgctggag aagaattaag aaccttaact caaaataact taaaaagtaa
     2041 aggtttatgg agcgatgctt atcaagagcg tctcgaaaaa gaattaacaa tcattcacaa
     2101 aatgggcttt gatgactatt tccttattgt gtgggattta ttaagatttg gtcgtagtaa
     2161 gggctactat atgggaatgg gacgtggttc agcagcggga agtttagtct catatgcttt
     2221 aaatattacc ggtatcgatc ctgtcaaaca taatttaatt tttgaacgtt ttttaaatga
     2281 agagcgttat tcgatgccag atattgatat tgatcttccg gatatttatc gtggagaatt
     2341 cttgcgctat gtacgtaatc gatatggttc aatgcattct gcacagattg taaccttctc
     2401 aacttttggt gcgaaacagg ctattcgaga tgtttttaaa cgctttggtg catcagagta
     2461 tgagttaaca aacataacta aaaaaattca tttcagagat aatttgacca gtgtttataa
     2521 ccgcaactta gctttcagac aaataataga tagtaaaatt gaataccaaa aagcctatga
     2581 tattgctaag cgtattgaag gaaatcctag acagacttct attcatgccg ctggggtagt
     2641 aatgagtgac gatttgttaa cagatcatat tcctttaaaa aacggtgagg atatgatgat
     2701 aacgcaatat gatgctagtt ctgttgaggc taatggtctt ctaaaaatgg actttctagg
     2761 tcttcgaaat ttgacatttg ttcagaaaat gaaggagaaa gtcgataagg actacggtat
     2821 ctctatacaa ttagaagcta ttgatttaga agataaagag actctaaaac tatttgcggc
     2881 tggtcaaaca aaggggattt ttcaatttga gcaaagtgga gctattaatt tgttgcgacg
     2941 tattaggcca gagtgttttg aagatgtggt agctactacc agcttgaata ggcctggtgc
     3001 aagtgattat actgagaatt ttataaaccg tcgttttggt aaagaaaaaa tcgatttagt
     3061 agatcctgtc atagctccaa ttttacaacc tacctatggc ataatgctct atcaagaaca
     3121 ggttatgcag attgcccaga cctacgcagg ttttacgcta ggcaagtctg atttgctcag
     3181 acgtgctatg tctaagaaaa attctaagga gatgcaaaag atgtctcaat ccttcttaga
     3241 gggtgcagta tctaagggac atcgtcaaga agatgctcga cttatatttg aacgcatggc
     3301 taaatttgca ggttatgggt ttaatcgcag ccatgccttt gcttattcgg ctttagcttt
     3361 tcaattagcc tatttcaaag cccattattc cgatgtcttt tatgacatca tgataaatta
     3421 ttcaaatagt gattatctaa tcgatgctat tgattttggt tttgttattg aaaaaccttc
     3481 aattaatact atttcataca gagaccgcat ctataaaaag aaaatttatt taggtttaaa
     3541 aaatattaag gctgttccta acgacttggc ttattggatt tctaaaaatc aaccatttca
     3601 gagtattgaa gattttctaa tgagactgcc acagcagttt caaaaaagtg gttttatttc
     3661 tccgctaatt gctattggag catttgacga atttgataac aatagacgga aaatcacctc
     3721 taatcttgat tctttattta catttgtaaa tgaactaggt agtttatttg cagatacctc
     3781 atatcattgg ttagaagtag aagatttctc taattctgag aaatatgaaa tggaacaaga
     3841 tatcttagga gtaggaatca gtccgcaccc tttagtgggg atttcacaaa aagctagccg
     3901 tccgtttata ccaattagtc aagtccaaga aaatagcgaa gctactattt tagttcagct
     3961 aaagcaggtc aaaatcatta ggacaaaaag ttctggacaa caaatggctt tcttgactgt
     4021 tatggatatt aattcaaaaa tggatattac tgtttttcct gaaactttca atattgtaag
     4081 agatgattta caagaaggga aatattatta cttgcatgga aaaattcaga agcgagatga
     4141 gcgattacag atggttttaa acggtgttca agaagcaacg gaggaacgtt tttggatact
     4201 tctaaaaaat catgataacg acaaaaaaat atcggaaata ttgtccaaat ataaagggca
     4261 tattcctgtc tatttgcatt atgaaacaac taaagaaact atccaaagca aagtacattt
     4321 ggttagaaaa gatagtggct tagcccttga tttatctgaa tttgttgtga aaacggttta
     4381 tcaataaaat tgcatataaa gtgcagaaat ttacaaatta atatgctata attaaatcgt
     4441 taaattaata aaaggagtac caattaaatg aaacgtattg ctgttttaac tagtggtggt
     4501 gacgcccctg gtatgaacgc tgctatccgt gcagttgttc gtaaagcaat ttctgaaggt
     4561 atggaagttt acggcatcaa ccaaggttac tatggtatgg tgacagggga tattttccct
     4621 ttggatgcta attctgttgg ggatactatc aaccgtggag gaacgttttt acgttcagcc
     4681 cgttatcctg aatttgctga acttgaaggt cagcttaaag ggattgaaca gcttaaaaaa
     4741 cacggtattg aaggtgtagt agttatcggt ggtgatggtt cttatcatgg tgctatgcgt
     4801 ctaactgagc acggtttccc agctgttggt ttgccgggta caattgataa cgatatcgtt
     4861 ggcactgact atactattgg ttttgacaca gcagttgcga cagcagttga gaatcttgac
     4921 cgtcttcgtg atacatcagc aagtcataac cgtacttttg ttgttgaggt tatgggaaga
     4981 aatgcaggag atatcgctct ttggtcaggt atcgctgcag gtgcagatca aattattgtt
     5041 cctgaagaag agttcaatat tgatgaagtt gtctcaaatg ttagagctgg ctatgcagct
     5101 ggtaaacatc accaaatcat cgttcttgca gaaggtgtta tgagtggtga tgagtttgca
     5161 aaaacaatga aagcagcagg agacgatagc gatcttcgtg tgacgaattt aggacatctg
     5221 ctccgtggtg gtagtccgac ggctcgtgat cgtgtcttag catctcgtat gggagcgtac
     5281 gctgttcaat tgttgaaaga aggtcgtggt ggtttagccg ttggtgtcca caacgaagaa
     5341 atggttgaaa gtccaatttt aggtttagca gaagaaggtg ctttgttcag cttgactgat
     5401 gaaggaaaaa tcgttgttaa taatccgcat gaagcggatc ttcgcttggc agcacttaat
     5461 cgtgaccttg ccaaccaaag tagtaaataa gaatttgtca atttgacact ttaaataaaa
     5521 aggagctcat aaattattat gaataaacgc gtaaaaatcg ttgcaacact tggtcctgcg
     5581 gtagaattcc gtggtggtaa gaagtttggt gagtctggat actggggtga aagccttgac
     5641 gtagaagctt cagcagaaaa aattgctcaa ttgattaaag aaggtgctaa cgttttccgt
     5701 ttcaacttct cacatggaga tcatgctgag caaggagctc gtatggctac tgttcgtaaa
     5761 gcagaagaga ttgcaggaca aaaagttggc ttcctccttg atactaaagg acctgaaatt
     5821 cgtacagaac tttttgaaga tggtgcagat ttccattcat atacaacagg tacaaaatta
     5881 cgtgttgcta ctaagcaagg tatcaaatca actccagaag tgattgcatt gaatgttgct
     5941 ggtggacttg acatctttga tgacgttgaa gttggtaagc aaatccttgt tgatgatggt
     6001 aaactaggtc ttactgtgtt tgcaaaagat aaagacactc gtgaatttga agtagttgtt
     6061 gagaatgatg gccttattgg taaacaaaaa ggtgtaaaca tcccttatac taaaattcct
     6121 ttcccagcac ttgcagaacg cgataatgct gatatccgtt ttggacttga gcaaggactt
     6181 aactttattg ctatctcatt tgtacgtact gctaaagatg ttaatgaagt tcgtgctatt
     6241 tgtgaagaaa ctggcaatgg acacgttaag ttgtttgcta aaattgaaaa tcaacaaggt
     6301 atcgataata ttgatgagat tatcgaagca gcagatggta ttatgattgc tcgtggtgat
     6361 atgggtatcg aagttccatt tgaaatggtt ccagtttacc aaaaaatgat cattactaaa
     6421 gttaatgcag ctggtaaagc agttattaca gcaacaaata tgcttgaaac aatgactgat
     6481 aaaccacgtg cgactcgttc agaagtatct gatgtcttca atgctgttat tgatggtact
     6541 gatgctacaa tgctttcagg tgagtcagct aatggtaaat acccagttga gtcagttcgt
     6601 acaatggcta ctattgataa aaatgctcaa acattactca atgagtatgg tcgcttagac
     6661 tcatctgcat tcccacgtaa taacaaaact gatgttattg catctgcggt taaagatgca
     6721 acacactcaa tggatatcaa acttgttgta acaattactg aaacaggtaa tacagctcgt
     6781 gccatttcta agttccgtcc agatgcagac attttggctg ttacatttga tgaaaaagta
     6841 caacgttcat tgatgattaa ctggggtgtt atccctgtcc ttgcagacaa accagcatct
     6901 acagatgata tgtttgaggt tgcagaacgt gtagcacttg aagcaggact tgttgaatca
     6961 ggcgataata tcgttatcgt tgcaggtgtt cctgtaggta caggtggaac taacacaatg
     7021 cgtgttcgta ctgttaaata atttttgaat tcaaacctat tatttaagaa taccttaaat
     7081 aatgggtttt ttatatgttt tgctttttat tattcgatta ttgtgactag aataggatat
     7141 gatattaata attac
//
LOCUS       DATROY010000097         6982 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.098, whole genome shotgun sequence.
ACCESSION   DATROY010000097 DATROY010000000
VERSION     DATROY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(51..869)
                     /locus_tag="VBD11_001540"
     CDS             complement(51..869)
                     /locus_tag="VBD11_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6954480.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
     gene            complement(957..1673)
                     /locus_tag="VBD11_001541"
     CDS             complement(957..1673)
                     /locus_tag="VBD11_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6954481.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            complement(1755..4373)
                     /gene="alaS"
                     /locus_tag="VBD11_001542"
     CDS             complement(1755..4373)
                     /gene="alaS"
                     /locus_tag="VBD11_001542"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6954482.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            complement(4389..4874)
                     /locus_tag="VBD11_001543"
     CDS             complement(4389..4874)
                     /locus_tag="VBD11_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6954483.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            complement(5116..6045)
                     /gene="prsA"
                     /locus_tag="VBD11_001544"
     CDS             complement(5116..6045)
                     /gene="prsA"
                     /locus_tag="VBD11_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6954484.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            complement(6106..6813)
                     /locus_tag="VBD11_001545"
     CDS             complement(6106..6813)
                     /locus_tag="VBD11_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6954485.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            complement(6887..>6982)
                     /locus_tag="VBD11_001546"
     CDS             complement(6887..>6982)
                     /locus_tag="VBD11_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6954486.1"
                     /translation="KSINLRLENSKENYNISSLKDIISLDFTRLD"
BASE COUNT         2114 a         1399 c         1156 g         2313 t
ORIGIN      
        1 accccaaaaa aagttactaa atagaaataa tttagtaacc ttttaaatct tcaattttta
       61 gttaaagcct ctttagccat aaacttataa aggcccgagt aacgtcctct ataatctttt
      121 ttccatggtt tatctcgtcc tgcaaaatgt aaaaaaacag tatggttaat aacccactct
      181 aaatcccatt cattgcgact ttttaattga taaataagac tatacctagc atcataatta
      241 taaatttcat caggtattgg tttaaccaaa ttagcgtata agccatttaa gatatcctga
      301 tctggtaaga ttagacgacc acggttttgc attatgtaat ctaagatagt ttgttgatga
      361 acaacttttc gtatagcagg caagttcatc aagagtaccc ctgtattgaa ataagaggat
      421 tctagctcta cattttttaa ccgtaattta ttgacataat ctagaaactt accatcagta
      481 ttatgacttg ccgctgcata tagttgatca cctaactcca tgtcatataa acttgagaaa
      541 tcatttaagc aaagcatatc tgcatctaaa taaagtatcc tatctaaggt ctcaggtaaa
      601 aatttatgag ccaaaagtcg ataatatatt gtatctggat agcgatcagt tgtaggtgcc
      661 tgagcaaata cttcggtacc tacaattatt ggatgatagc caacttctaa attttgagta
      721 tattgaataa gttctgtatg acgtttgagt aatgtttttt gtaagacata tatctctaac
      781 ttacgattct tggtttgtcg gacaagcgaa taaagcatca ctttaaaatg atctacgtac
      841 atatcatcaa tagaaaataa tagattcatg agttggatcc tctccgtagt atatcttctt
      901 tcatcctaac atatgtctaa agaaaaacca agtttgagtt aaaacttggt tatcttttat
      961 ataaaacttc cccataatat agctaaaata attgctggca acatattgat aactttaata
     1021 tttaggttaa agagtaaatt aacaccgaca cagaatataa gcatatttcc tactaagtta
     1081 aggtaatcta aagatgatgg attaagtagg ctccccatga aaaaagcaac aatcgtaagg
     1141 ctaccttgta gtaaagcgac aggaagtgca gaaaatagag cccctttccc taaagacact
     1201 gtcataattg caataataat catgtctaac attcccttag caaataaaat cgaatgatct
     1261 gctgctatac catcttgaat gctaccaaca acggccattg ctccaataca aacagtacaa
     1321 gttgaggtga cgaatgcttc cacaaattta atatcgttac cacttcctgt tttttgctta
     1381 agatagtccc caaatctatc aatatacgaa tctagtgata gcaactctcc aagtaccgta
     1441 cctagagcaa gagtaataat catcatattt gtatgattag agatgagatg tgacttttcg
     1501 actaacatca ttttctccaa aacacctgat attgaaataa ataaaactgc gactcccatg
     1561 gcttgcatga ggctcttttg aagtgattct ttcaaaaaat ttttcaaaaa taaacctaca
     1621 aagcctccaa ctataataag aataacattg ataactgttc ctaaaccaac catatttctc
     1681 ctatatacac aaaggaacag aacgatgttc tgccctttgc ctgttttatt tactaactaa
     1741 atgagaggta cttcttataa attttctgca actgctgcta gcagctctgc aattttactt
     1801 gcatcgctac cacctgccat agccatgtct ggtttaccac caccacgacc tgctacaatt
     1861 ggtgccaatc ctttgatcat gttaccagcg tggacatctt tagttttgct tgcaacaagg
     1921 acattaacct tctcaccaat agctgctacg agaacaagca catcagagta gtctttttgt
     1981 ttccagttat cagcaaatgt acgaagtgca cctgcgtctg caacatcaac ttgactagca
     2041 atgaagcgca cgcccttagc ttcttgaata tctttaaaga catcaccagc tgctgctgct
     2101 gctgcttttt ctttaagctc tacattttct ttttgaagat cacgaagtga atcgctaaga
     2161 gcttgtactt tagctgttgc atctttcaat tgcggagctt ttacagtagc agcgatctct
     2221 tttagtgcat cctcttggtt acgataagct tcaaaagctt gtctaccagt aactgcaata
     2281 atacgacgag tgcctgaacc aataccttct tctttgacaa tcttgaagag accgatttct
     2341 gaagaattat ttaagtgagt tccaccgcaa agttcaacag aataattacc aatttgaacc
     2401 acacgaacca ctttaccata tttctcacca aaaagtgcca ttgctcccat ctcttttgcg
     2461 gtttcaacgt cagtttcagt cgttgtgatt gtaagagcgt tccaaatttg ctcattaact
     2521 tcttgttcaa tgtgacgaag ttcctcattg cttactgctt caaagtgagt aaaatcaaag
     2581 cgcaagaatt cttcttcgtt caatgaacca gcctgagttg cgtgttcacc gataacattg
     2641 tgaagggctg catggagcaa gtgagttgct gtgtggtttt tctcaacagc caaacgacgc
     2701 tctttgttga tttcaagtgt gtagtttgtt ccaactgaaa gtgatgccaa aacgtttaca
     2761 gtgtgtagag gttgaccatt tggtgctttt tgaacatcaa caacctcagc aactgtgtca
     2821 cccttatcat ttttgattct acctgtgtca gcaacctgtc cacccatttc agcatagaat
     2881 ggtgtttgag caaagacaag aagggcttga ccttctgaaa cagcctcagt acgttcatta
     2941 tcagcaatga taactgaaag acttgattca agactatatg tgtcgtattc gaatcgtgat
     3001 tcttcaacaa taccagctag ggtttcattt tgcatcccca tcgaaccacc cttaacaaca
     3061 gctgcacgcg cacggtcttg ttgttctttc atggctgact taaagccttc gtggtcaatc
     3121 ttatagcctg catcttctgc caattcctct gtcaattcaa ctgggaatcc ataagtatca
     3181 taaagtttga agatatcttt accttcaagt gtgtctttac cttcagcctt aagctgcgca
     3241 agcaatgaat ctaagtgacc gctaccagca tcgatagtac gagcaaatgt ttcttcctca
     3301 cgtttaacga ttttctcaat aaaatcacgt ttttcaagca cttctggata gtagctttcc
     3361 atgatttgtc caacagtcgg aaccaatttg taaaggaaag tttcgttgat gccaagacgg
     3421 cgaccgtgca taaccgcacg acggagaaga cgacgaagaa cataaccacg accttcattt
     3481 ccaggaagcg caccatcacc gatggcaaat gaaagcgcac ggatgtgatc agcgataacc
     3541 ttgaagctca tgttgtcgcc atctggatcg taagttttac ctgacaattt ctctacttca
     3601 cggatgatag gcatgaaaag gtcagtttca aagtttgttt ttgccccttg cataacagct
     3661 gcaagacgtt caagacccgc acccgtatca atgtttttgt ttggcaattc tttgtattct
     3721 gaacgtggta cggctgggtc agcattgaat tgtgagagaa caatgttcca gatttcgatg
     3781 taacgatcgt tttcgatatc ttcagccaaa agacgaagtc caatattttc tggatcaaaa
     3841 tcttcaccac ggtcgaagaa aatctcagta tctggacctg aaggaccagc accgatttcc
     3901 cagaagttat cctcgattgg caccaagtga cttggttcaa cgccacaagc aatccaacgg
     3961 ttatatgaat ccttgtcatc tgggtaataa gtcatgtaga gcttgtcttt agggaagtca
     4021 aaccattcag gacttgtcaa gagttcaaat ccccactcaa tagcttcatc acggaaatag
     4081 tctccaattg agaagttacc aagcatttca aacatagtat ggtgacgtgc tgttttacca
     4141 acattttcaa tatcattagt acgaattgat ttttgtgcat tggtaatacg tggattttct
     4201 ggaatcactg aaccatcaaa atattttttc aaggttgcaa cacctgagtt gatccaaaga
     4261 agcgttgggt cattaacagg aaccaagtta gctgaaggct caacggaatg tcctttggat
     4321 ttccagaaat ccaaccacat ttggcggatt tgtgcagacg ataattcttt cattattctc
     4381 tcctaaaatt agtttgatgc atttttttct aactctttga catagtcgat cgctatgaca
     4441 agcgaaattg tcaaatcgtt gtaagtttca ctgtaaactt ctacctgata agttgaagtc
     4501 atacgaaacc actgttgaga tatgtttgct ataacttgat tgtcttttaa gagttggaaa
     4561 tccatatccc aaaaatttcc cttaacttcc atatcaaggt cttcaatggt gtaatgaggt
     4621 ttcaaaaatg agaaatcttt cttaatcgtg aaatgattcc cattggctaa tgtcacatta
     4681 aactttggta aaaaacttaa aaccttcttc tcaatctgac taatgagatg ttcctcttcg
     4741 tcaaagactt taaaagtctt aggtagaggg aacaaacttc cttcaacgtg ataacaggga
     4801 agcccagtca ggtctgtaat agtaaattta ccgcctagag aaaacatttt ttctttaatt
     4861 tgaaaagtag ccatatctct tcctcctcga aagttgacaa atagcaaaag acaagtccat
     4921 tacgaagggc gaaaaccgcg gtaccacctt cattcaatga acttgtcatt ctcatttatt
     4981 tttagtttta ccccattgag tagcatcttt ttctgattat ccatgactcg cagcaaccgt
     5041 cacttttctg tatctaatcg ttgaaatttc attctcaata gtattaatta tacagtcttt
     5101 ttaagacttg gtcaactatt ttttaagtga tgatgaacca cttgttgtag catattgtga
     5161 taagatgcct gcaaatgctt tatctttaat cttaacgtta gctttttcta aggcttttga
     5221 aatcacttta ttttggaagg atctatcgct tgttttatcc ttaagaataa cttcttttaa
     5281 gcgatttttg taagatttcc aatcagattt tttctctgtc ttatcagtta ctttaatgat
     5341 gtagtaactt gttttataag ttgttgaatc aaccgttgaa accacatctg aaacaccatt
     5401 tttatctagc ttaaaggctg ctgacataac ttctttaggg aggcttgtcc ctgcagaatc
     5461 aaatttatac tcaacttttt tatcagtagc tgttgttttt tcttttgcaa tctttgcaaa
     5521 atcagctcca tcagccttta catctttaag gacagattta gctttatctt ctgcatccaa
     5581 tttgattact tgtacagaag tttcaggagt atagttttta tatgcttctt tatagtttgc
     5641 ttctgttaat tctttcttag ctgcttcttt tacagcatat tccacaagca tagttgtgcg
     5701 aatttgttgt ttgtaacctt ccggagtcaa acctgcttgt gaaagtgcgc ttgaaaatga
     5761 attaccatag cctttagctg tcttattata agcttctgat acttttttat ctgaaacttt
     5821 atcaccatac tgtgtatcaa aaacacgtga gaggatcaat gtaagcattg attgttgtgc
     5881 agcttttgat gtttttactt gatcataaaa atcagagact gtaatagtgt cgcccttcat
     5941 tgtaacaaca tttgttccat ttgatgtttt acctgagcag gcagctaatg tggcaactga
     6001 cataagtgtc aaaaagcctg ctgcaagttt tgatcttgtt ttcatgattt ataactcctt
     6061 tttgtcatta actccattat tatatcataa tttcttaaaa atgccttaat cttctagtac
     6121 aatatgatct gcatttttac gaatcatcaa taaaccatct cccaaaggta ccaaagtagc
     6181 agttaaatca ggatgttgca aagtcgagtc aaataaacgt tgcagacctc ggtagatagt
     6241 tcgctgaccc cttctcactt catcgatagg ttttgcaata tctccacctt ggaaaatatc
     6301 atctaaaaca actactcctc caacatctaa atgctttaat acttgaggta aaaaaacgat
     6361 atatttagac ttagcagaat ccataaatac aaaatcgtaa gatttatcca atgtttgtaa
     6421 cacatccaca gcatcgcctt ccaaaagggt tatctgattg tgattatcat attttgcaaa
     6481 gttttcctta gccaaggcaa tcatctcttc attacgatcg attgttgtta tctttgcttc
     6541 aggagcattt tctgccatca aaagggccga aaaaccaata gctgttccga tttctaaaat
     6601 gtgcttaggt tgtaatgttt gcatcaaaaa acgaaaataa gtcgctgttt catgaggtat
     6661 aatggggata ttttcctgtt tagcaaatgc ttctaactct gctaaacagc cattattttg
     6721 tttttggtgc tgacgcatat attgaacaat ttcttcttta actactggac ggcgcatatt
     6781 atgattagca tttttactgt aagattgaac cacttttggc tcctcttttc tattttaaat
     6841 aataactttt ttattatagc aaaaaatgtc taactctgag aatgatttaa tccaaacgag
     6901 tgaaatcaag tgatattata tctttgagac ttgaaatatt atagttttct ttggaattct
     6961 ctaaccttaa gtttatggat tt
//
LOCUS       DATROY010000098         6772 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.099, whole genome shotgun sequence.
ACCESSION   DATROY010000098 DATROY010000000
VERSION     DATROY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            43..681
                     /locus_tag="VBD11_001547"
     CDS             43..681
                     /locus_tag="VBD11_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6954487.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVASISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(759..1544)
                     /locus_tag="VBD11_001548"
     CDS             complement(759..1544)
                     /locus_tag="VBD11_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6954488.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            1805..3250
                     /locus_tag="VBD11_001549"
     CDS             1805..3250
                     /locus_tag="VBD11_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6954489.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(3307..3978)
                     /locus_tag="VBD11_001550"
     CDS             complement(3307..3978)
                     /locus_tag="VBD11_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954490.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(4075..4395)
                     /locus_tag="VBD11_001551"
     CDS             complement(4075..4395)
                     /locus_tag="VBD11_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6954491.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(4404..4532)
                     /locus_tag="VBD11_001552"
     CDS             complement(4404..4532)
                     /locus_tag="VBD11_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6954492.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            complement(4633..5427)
                     /locus_tag="VBD11_001553"
     CDS             complement(4633..5427)
                     /locus_tag="VBD11_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954493.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(5437..6666)
                     /locus_tag="VBD11_001554"
     CDS             complement(5437..6666)
                     /locus_tag="VBD11_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954494.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
BASE COUNT         2084 a         1270 c         1057 g         2361 t
ORIGIN      
        1 tccaagtctc tgcactttag ttgatcaata aaggagactc ctatgttttt aatcgatttt
       61 attttacata ttgatactca tatctatgct atggctaaca ctgttggtaa ttggacctat
      121 ctattgctct tcctcgttat tttcgttgaa actggggctg tcatctttcc atttttacca
      181 ggtgatagtt tattatttgc tgctggagcc cttgctgcaa atccaaaaat gtccttcaat
      241 attgtaactt ttcttattat tttctttatt gcagcattta ttggtgatag ttgcaacttc
      301 ttgattggtc gtacatttgg ttaccgtttt atcaaacatc ctttctttcg tcgtttcatt
      361 aaagaaaaaa atatccgaga tgctgagctt tattttgaga aaaaaggtac tgctgccatc
      421 attttagggc gctatatccc tattatcaga acctttgttc cttttgtagc aagtattagt
      481 cagctccctc caaaagtatt tatcaaacgc gccttcattg cagcattatc ttggtcagtt
      541 attgcaacag gttctggttt tctctttggt aatattcctt ttgtgaagca gcacttctcg
      601 cttattatct tgggaattgt atttgttacc ttaattcctg tccttatttc tggtgtcaaa
      661 tcatatcgga acactaagta atgtatccaa aaatttctat ttaacactat taatcactaa
      721 gaatgacaac aaaaaaagag caattagttg ctcttttatt aatttagtgt tatttctcct
      781 gttgcataat ccatagtgta tgatgcttgt gtgtttggaa tagcaacatt aaattctaat
      841 gtgccatctt gtgatttagc ttctaggtgc attgcaaatg gaactaaatc agtatcatta
      901 cggtacaatg gagttacacg gtaacgaaca cgtttgtttt ggtcaaccgc cttacgaact
      961 aagctttcat aataattttg tccacgattg attttttgat ttgattggtt ggaatgagct
     1021 gtttgtgtga caacattttg aggatttgac acggaagcat cccaaccttt gaaatttcca
     1081 gctaaagcat aggcaattaa atgccccttg tcgactgcat gcccataatg gtcattagta
     1141 gctacttgat gccaacctaa tggtttccaa tttgcatcgg gaagagtggt atctctgcga
     1201 ttttgatatt gacgaatggt tctatcaagc attgcattag cagttgatag tctttgttga
     1261 ttgtcaattg tattttgaac atacgcttta acattgacat cagcatttaa atttgtctga
     1321 ttattattta attcataaat accattaaca tattgaaatg gtaggttacc aatagcattt
     1381 tttaaattat tttgaaattc ctggctcgta tcaacagatg aattgacatc agcatgtact
     1441 atcggcgtta cgttttgcgg ctgcgttgga agaccaaagg tcattacccc caacatacct
     1501 aatgtagcaa ttacggttat aaacagttta tgtaatcttt tcataagctt tacctctttc
     1561 taattacatt aaacaagcgt ctaccatatt gatatccgtt ttagctaatt gctctccttt
     1621 tgtcacaacc atatcatatg cctattattt aagcatgtca atcatttatt taatcttata
     1681 ttaatttaat attactttaa atttaattta ctcgttaaat tagaaaatct gcaaataata
     1741 gctttgttca tcccctaact taaatcaaaa tttatgctat gatagtggta aggaggcttg
     1801 ttctgtgaaa aaaacaattg gtattatagg tatgggagtt agtggtttag ctgttctact
     1861 agcactttct cgtaaaaaag agaaagagtt aaaacagtta gctatttttt gttttgatga
     1921 tactgtccac tttggacgag gaattccttt tcaagaagac gttgactcag ctttaatcaa
     1981 ttccccaatc aacgatattt catttgatta tcgccatatg tctgacttca tggattggtt
     2041 agaagaaaat tactatgaca caaatctaac ctatgtatca aggacacttt acggacaata
     2101 cctttcagaa agaactaagt tacttttaga taaactccct gtcacggtta ttaaaacaag
     2161 agtggataaa ctaaactatt tatcagatga gcaacaatgg caagtctata ttcaagataa
     2221 gctttttcct gtcacatttg atgaattaca cttagcttgt ggtgctctgc ctgttataga
     2281 accttatcat ttggaaggga agaccgatta tatcaaagac ccttatccta tcaaatactt
     2341 ggctcaggaa aattggaagg gaaaaacagt tgctatcatc gggactggtc tagctgcaat
     2401 tgatgttata aaatggttgc tactcaatac acaatcttct gttaaagcat tttcaagaac
     2461 caactacttc cctagcgtcc gtattgttaa tgatttacca cttacttggc acttttttac
     2521 caatgataac cttaaagcat ttctatcaca tccttcaaaa accttcacta ttgccgattt
     2581 taatgatttg atgtctggcg aattggagca tcttggcttt aaaaactgga aaaatacaac
     2641 agaaactttt ttatccccag gaattgatgg tctcaagtta gcctttaaat accctaagca
     2701 actctatcta ttacaacaat tagcttcacg aatcactgtc tggttaactg acctttggcc
     2761 actcatgtca ttatcagata agaaagagta taaagcactc tatggaaaag cgattattaa
     2821 cttgcgaaat cctatgcctg atgaatctgc taaagtaatt ttgaaagctg cacaaaaagg
     2881 gactttttct ctgatcaata acgtcaagac aattacaaaa aatgacgaag gattttcaat
     2941 agaaaaagaa aaacaaaatc atatatctgt cgatcgcgtc attaacgcta ctggttacca
     3001 gttaaacact aaaaactgga caaaagccaa tccacttcta aaatcgctca tcaatcaaga
     3061 attttgtcaa attgacgatc aaggagggat tactatttta actgaaaatg cacaagtttt
     3121 gtctccaaaa tatggtccaa tgccaggttt atatgcgcat ggtgcactgg ttaatggtgt
     3181 cgtttatcag aataactcta caattaaaat tcaacaaatg gcagagcgag ccttttctga
     3241 taataaataa ccatttctaa aaaatacctc ctcttttatt atttaagaaa gggaggtatt
     3301 tttttattat aaattccttt gttgtaatat agactgtgct atactttctt ttgataattc
     3361 ttttaagtta tctcctatca attgtaaagc agtatcagcc atatgagcga tctctctact
     3421 atgtgtcacc ataataacac atcgtttttc cttatgcgca aaatctttaa gacgcatcgt
     3481 aatatcacgc gcaatgtcaa aatctaaatt tcccgtaggt tcatcaagta aaatagctgg
     3541 tgttcctaca accaaagctc tcgcaatagc tacacgttgt tgttgccctc ctgataatct
     3601 caagatattt cgcgttaaca tatcttcttt taaacctaaa tccaacaata attgtttatc
     3661 tgcagtaggt tttaccaact gaacattttc caatggggtt agatagtcta aaaggttata
     3721 attttgaaag accaaagcta tattcttttt gcgatgctga gcataacctg cttctttaat
     3781 atcttttttg ttaaataata cttgtccctc agtaggcgaa tcaagacctg ccatcaagga
     3841 tagaagggtt gttttccctg aaccagaatt ccctaatata gtataaaata cccctgactg
     3901 aaaactataa ttgatatttt ctaatgtttt attgtcgttc cccttataac gataagcaat
     3961 attttttaat tcgagcatag atttctcttt cttgagctat gtattcaata gcagagcata
     4021 ttcattttaa ccgttaatat agatgtcact attggtcggt atacttatgg cactttagga
     4081 cagttttgat aagattcccc ttggtttgta gcgagcaata attaaactag tagtaataat
     4141 agcaaataag acgattgcta aaccaactag aattactaac cagacttcct caggcttcac
     4201 ttgtacattt aacgtcttaa tcgtttgcat aaacatatct gtatcactat cagccccaag
     4261 ctgcatattc ccaaaccctt tcataaagtc tgctctgaca gttctagagg cctgatacac
     4321 taaggagtct gctaattttt gaccaataaa ttgactgata agtactgata aagcaaaggc
     4381 tggaatggca atcattagta cttctactgc gtactgacaa tgataattgc tttagactta
     4441 ccaatagcta ataaaatacc tgtctcatgg gttctaccac caatccagaa tagcagagcc
     4501 atcatcacta ggataataga tactactaac atgccgcctt gtagagaagt aattaattga
     4561 ttcaagacat ctatactttt tgcataactt gttagcaagg ttgctgtaat tgtcagttga
     4621 tatttattcc aatcaatagg attgtttttt gataaaactt ttaagagttt ctatatcagc
     4681 attcttttta ggatatacag ttgtttggcg ataaaaggcc ttacctttag aatagccgta
     4741 taaatcctta atcaattgcg tatctgaaaa ggctgtattt tctagcatct cataagggta
     4801 atttgatttt tgtaccgttt tacctttata tatgccaaca atagttgctt caactaatgt
     4861 tttatcctta ctgtttctaa cggggtcttt tgaaaacatt aacttatcgc caactttaag
     4921 atgatttgtc ttcgcaaaag cctgatgaac aattataact cgctgatctt tcggcgtgat
     4981 atgtcgccct cccttaagag aaatcatccc cgctgtgtat ttaggatctg tatcggagta
     5041 actattacca tgaacttgta aaacttgttc tttatattgt tctttatcat ttggtcgtgt
     5101 aaccattttt tccgaggtta gtccagcata agccatcaaa ttattattaa cgcgcttaac
     5161 atttggatta cttgctatgt taactattgc tttttcaggg atttctccag atccgtaacc
     5221 atcaccaaca aggtcagtac tatccgatga aatatgcaaa attgatccag tttgccttga
     5281 aaatgtcttc ttagcagtaa gaccagcatt ttttaccgca ataccgctaa ttaatgtaga
     5341 agcaactagc cataaacata cgaataaagt gatactctta cgctttttac ggctaacata
     5401 taaaaaggaa cgtttaaata aattcatatt aacctcctat gacattcgtg acaatatttg
     5461 ctttggttgt agtttaagaa tagaataact agataataca actgcaaata agcaaattaa
     5521 agcaccctga gcatagagtc taaagacatc cattactcca acagaaacag cgaggttatc
     5581 aattctatca ataattagac tactattttg tacaatttgt gaactcacac caccattaac
     5641 ttgtgataaa aggccagctg caaatgtttt tccaagaaag acaccaccaa taatagatag
     5701 tgtgaaggca gcacctgcta ctaacagtac ttctatcaga taatgaccta tgatttcaaa
     5761 cttagttttt ccgatagcga gtaaaattcc cgtctcttta atgcggttat taatccaaaa
     5821 tgttagcatg acaactaata ttgccatcgc cacgagacta acaataagaa tgattcgtcc
     5881 cactagacga tgcaatgtct cgatattacg ataagaatct ccataaaact ctttagctgt
     5941 ttgaacatct aatttcccga aatcaagcgg ccattgtttg atgttttgga taactgtatc
     6001 cactaaacgt ttatcactta agcgcactgc aaccgtctga taaccttttt gtttactaat
     6061 cttcctaacc aatgttgcct gagcaatcaa tgtattatca atattgggat taaaagtatt
     6121 tttagactta gcactattcg catcataaat tcctgcaata gtaacatttt ctttgcctaa
     6181 tctcaatttg ttacccacac ttaaatgatt cttttgtgct aacttcaatg gaactagtac
     6241 ttgttttgta ttatcggccg ctaaatggct tcctttcttt aagaccaatg cttttgtctg
     6301 aaaagctcgt aaatcttctg tactttgata acctgctaat gtaacttgat tctcttcttt
     6361 agaagagtta tatggtgata cagattttaa acggctacca acatcaatgc ttaaacttgc
     6421 ctctactgac tcaaccattt ttgctttttt caaagcttcg tatgcttgtt caggccaata
     6481 agccttttct ttggaggtta aataaatgct tttcccttgt tttgttagga tatttttttc
     6541 caaagaatgc tgaatactca tcagactgat taaacatgtg cctatagtta gaaaaactaa
     6601 gaacaaggtt actgttttca ttttttgacg tttcaaataa gctaaactta atgtccatag
     6661 gttcactatt ttccctcctt tttctatctt aataatctct agaataacga tagaagtcgt
     6721 tagatattct ctatcgactt ctacgttatc tatcaattag tgattttata gc
//
LOCUS       DATROY010000099         6699 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.100, whole genome shotgun sequence.
ACCESSION   DATROY010000099 DATROY010000000
VERSION     DATROY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            181..381
                     /locus_tag="VBD11_001555"
     CDS             181..381
                     /locus_tag="VBD11_001555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6954495.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            413..1129
                     /gene="atpB"
                     /locus_tag="VBD11_001556"
     CDS             413..1129
                     /gene="atpB"
                     /locus_tag="VBD11_001556"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6954496.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            1147..1644
                     /gene="atpF"
                     /locus_tag="VBD11_001557"
     CDS             1147..1644
                     /gene="atpF"
                     /locus_tag="VBD11_001557"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6954497.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            1644..2180
                     /locus_tag="VBD11_001558"
     CDS             1644..2180
                     /locus_tag="VBD11_001558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6954498.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            2196..3701
                     /locus_tag="VBD11_001559"
     CDS             2196..3701
                     /locus_tag="VBD11_001559"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6954499.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            3717..4598
                     /locus_tag="VBD11_001560"
     CDS             3717..4598
                     /locus_tag="VBD11_001560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6954500.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            4672..6078
                     /gene="atpD"
                     /locus_tag="VBD11_001561"
     CDS             4672..6078
                     /gene="atpD"
                     /locus_tag="VBD11_001561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6954501.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            6091..6504
                     /locus_tag="VBD11_001562"
     CDS             6091..6504
                     /locus_tag="VBD11_001562"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6954502.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            6569..>6699
                     /locus_tag="VBD11_001563"
     CDS             6569..>6699
                     /locus_tag="VBD11_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954503.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     "
BASE COUNT         2143 a         1116 c         1371 g         2069 t
ORIGIN      
        1 catatcttaa taagggatta aattaaagct aaaaaaacta aaaattgtta tttgaaaagg
       61 attgtgtaaa ccatctcaaa ctatttcatg aaaacgtaat caaatgagga aattcattga
      121 catgcacaag aaataggaat aaaataacgg agtaaatatt ttttaaagga gaattctatt
      181 atgaatttag ctattttagc acttggtttc gccgtgatgg gtgtttcaat cggtgaagga
      241 attttggttg caaatattgc caaatcagcg gctcgtcagc ctgaaatgtt tagcaaactg
      301 caaacattga tgtttacagg tgtggccttc attgaaggta ccttcttcgt tctatttgcg
      361 tttacattct tagtaagata gacgacaatt tagaaaaaag gagggatgag gattggaatc
      421 tacaagtaat ccaaccgtat cttttttagg catcgatttt gatttaacca tcctagctat
      481 gtctctcttg acaattacta ttatctttat cttagtcttc tgggctagtc gtaagatgac
      541 aataaaacca aaaggtaagc agaatgtctt agaatatgtc tatgagcttg ttaataatac
      601 aatctcacaa aatttaggac attatactaa aaattatagt ttattgatgt ttattttatt
      661 cagttttgtc ttcattgcta acaacttagg attgatgaca tcattgaaaa cacatgagca
      721 taatttttgg acatctccga ccgctaattt tggtgtagat attacattat cattattagt
      781 agcatttatt tgtcatattg aaggtattcg caaaaaagga attggtggtt atctcaaagg
      841 tttcttatca ccaacaccag caatgcttcc gatgaattta ttggaagaag ttactaatgt
      901 tgcttcatta gcattgcgtc tttttggtaa tatcttttct ggtgaagttg tcactggatt
      961 acttttacaa cttgcagttt tatcaccatt tacaggtcca ctagcttttg cattgaatat
     1021 cgtttggaca gcattttcaa tgtttattgg tttcatccaa gcgtatgtgt tcattatctt
     1081 gagctcaagt tatattggac acaaagttca tggtgacgaa gaagaataag aaaagagagg
     1141 agaaatatgt caatacttat taatagtaca acgattggtg atatcattat cgtatctggc
     1201 tctgtgcttc tcttatttat cttaattaag acttttgcat ggaaacaaat cacaggtatt
     1261 tttgaagccc gtgaacaaaa gatagctaat gatattgata ctgctgaaca ggcacgtcaa
     1321 caagctgaag cttttgcaac taagcgtgag gaagaactat ctaacgctaa aactgaagct
     1381 aatcaaatca ttgataatgc taaagagaca ggtcttgcaa aaggtgatca aattatttca
     1441 gaagcaaaaa ctgaagctga tcgattaaaa gaaaaagcac atcaagatat tgcccaaaat
     1501 aaggcagaag cactagcaga tgttaagggt gaagtagcag atttaacagt tttattagct
     1561 gaaaaaatta tggtatcaaa tcttgataaa gaagctcaga gtaatctcat tgatagctat
     1621 attaaaaagc taggagatgc ctaatgaata aaaagacgca ggctctaatt gagcaatatt
     1681 caaaaagcct tgttgaagtt gccattgaac ataaaattgt tgaagaaatc caacaagaag
     1741 tagcagcttt aattgatatt tttgaaacaa gcgagcttga gggtgtcctg tcaagtcttg
     1801 ccgtttcaca tgatgaaaaa caacattttg tgaagacatt gcaaacatca tgttcaactt
     1861 atcttgttaa tttccttgag gttattgtac aaaatgaacg tgaagcactt ctatatccta
     1921 ttttgaaatc agttgatcag gaacttatca aagtgaatgg tcaatatcca attcaaatta
     1981 caactgcagt tgctttgtct ccagaacaaa aagaacgtct ttttgatatt gctaaaacta
     2041 aattagcttt accaaatgga cagttagttg agcacattga cccatctatt gttggtggat
     2101 ttgttgtcaa tgcaaacaat aaagtaattg atgcaagtgt tcgtaatcaa ttacatcaat
     2161 ttaaaatgaa attaaaatag aaagtggtgt gacttttggc aattaatgca caagaaatta
     2221 gcgctttaat taaaaagcaa attgaagact tccagccaaa ttttgacgtc acagaaactg
     2281 gtattgttac atatatcggt gatggtattg ctcgtgctcg tgggctagat aatgctatga
     2341 gtggagaact tcttgaattt tcaaatggtg cgtacggtat ggctcaaaac cttgagtcta
     2401 atgacgtggg tatcattatt ttgggtgatt tctcagaaat tcgtgaaggt gatgtggtaa
     2461 aacgtactgg taaaattatg gaagtaccag taggggaagc tatgattgga cgtgtcgtca
     2521 atccgcttgg tcaaccagtt gatggactag gtgaaattga gacgacagca acacgcccag
     2581 ttgaaacacc agcacctggt gttatgcaac gtaaatcagt ttttgagcct cttcaaacag
     2641 gtctcaaagc gattgatgct ttggttccta tcggacgtgg gcaacgcgaa ttgattatcg
     2701 gtgaccgcca aactggtaag acgtctgtag cgattgatgc aatcttgaat caaaaaggtc
     2761 aagatatgat ctgtatctat gtggctatcg gtcaaaaaga gtctacagta cgtacacaag
     2821 ttgaaacttt gcgtaaatat ggtgcccttg attacacaat cgttgtgaca gcttcagcat
     2881 cacaaccatc tccattactt ttcattgctc catatgcagg agtagcaatg gctgaggaat
     2941 tcatgtataa cgggaaacac gttttgattg tttatgatga tttatcaaaa caagccgtag
     3001 cttatcgtga actttcgctt cttcttcgcc gtcctccagg tcgcgaagct tacccagggg
     3061 atgttttcta tcttcatagt cgtcttttgg aacgttcagc taaagtatca gatgctttag
     3121 gtggaggttc gattacagcg cttccgttca ttgaaacaca agcaggtgat atctcagcat
     3181 atattgctac aaacgttatt tcaattactg acggccaaat tttcttgcaa gaaaatctct
     3241 ttaactcagg tattcgtccg gcgattgatg caggttcatc agtatctcgt gttggtggtg
     3301 cagcgcaaat taaagcgatg aagagagttg caggtacgct tcgtctagat cttgcttctt
     3361 atcgcgaact tgaggcattc actcaatttg gttcagacct tgatgcagca acacaagcaa
     3421 aactaaatcg tggtcgtcgc acagtggaag tgttaaaaca accattacac aaaccattgc
     3481 cagttgagaa acaagttgtg attttatatg ctctaacaca tggattcctt gatgatgtac
     3541 ctgtaaatga tattttagct tttgaagaag ctttgtatga ttactttgat gctcattatg
     3601 ataatctctt cgaaacaatc cgcacaacaa aagatttacc agaagaagca gaattagatg
     3661 ctgctattca agcatttaaa gatcaatcac aatttaaata ataggagggt tctaatatgg
     3721 caggctctct tagtgaaatt aaagacaaga ttctttctac agaaaaaaca agtaagatta
     3781 ctagtgcgat gcagatggtc tcatctgcca agttggttaa atctgaacaa gctgctagag
     3841 attttcaagt gtatgcctct aaaattcgtc aaattactac taacctcttg aagtcagact
     3901 tagtatctgg atcagataac cctatgttag cttcacgtcc ggtgaagaag acaggttata
     3961 ttgttattac ttctgataag ggtttagttg gtggttacaa ttcaaaaatc ttaaaagcga
     4021 tgatggacac tataactgac tatcatactg agaatgatga ttatgccatt atttcaattg
     4081 gtagtgttgg ttctgatttt ttcaaagcac gtggaatgaa tgtttctttc gaattacgtg
     4141 gtttagagga ccaaccaagt tttgatcaag ttggtaaaat tattgctcaa gcagttgaaa
     4201 tgtacaaaaa tgagttgttc gatgaattat atgtttgcta caatcaccat gttaacagtt
     4261 taactagcca agtcagaatg caacaaatgc ttcctatcaa agaattggat gcagaagagg
     4321 cttctgagga tagagtgatt actggttttg aacttgagcc aaaccgtgaa gttatattgg
     4381 agcaattatt gcctcaatat actgagagtt tgatttatgg agctattatt gatgctaaaa
     4441 cagcagagca tgcggctggt atgacagcta tgcagactgc aaccgataat gctaaaaatg
     4501 ttatcaatga tttaactatt caatataacc gtgctcgtca agcagctatc acacaggaaa
     4561 ttacagaaat tgttgcaggt gctaatgcac ttgaataatt aattagtcta tagaaaaaat
     4621 agtgaacaaa ggtattttaa atcctttgta tcatatgata aggagaaata catgagctca
     4681 ggcaaaattg ctcaggtagt tggacctgtc gttgacgttg tatttgcaag tggcgacaaa
     4741 cttcctgaga ttaataatgc attgattgtc tataaaaatg gcgataagtc acaaaaagta
     4801 gtacttgaag ttgctcttga acttggtgac ggcctcgttc gtacaatagc tatggaatca
     4861 actgatgggc ttacacgtgg tttggaagta ttagatactg gtcgcgcaat tagtgtgccg
     4921 gttggtaagg atactttggg tcgtgtcttc aacgttcttg gagatgctat tgaccttgaa
     4981 gagccttttg cagaagatgc agaacgtcaa ccaatccata aaaaagcacc atcgtttgat
     5041 gaattatcaa catcatcaga aatcttagaa acaggtatta aagttattga cttattagca
     5101 ccttacttaa aaggtggtaa agtcgggctt ttcggtggtg cgggtgttgg taaaaccgtt
     5161 cttattcaag aattaatcca caacatcgcc caagaacatg gtggtatttc agtatttact
     5221 ggtgtaggag aacgtactcg tgaagggaat gacctttatt gggaaatgaa agaatctggc
     5281 gttattgaaa aaacggctat ggtctttggt caaatgaatg aaccaccagg agcacgtatg
     5341 cgtgtggcac ttactggtct tacaatagct gaatacttcc gtgatgtaga aggacaagat
     5401 gtgcttctct tcattgataa tatcttccgt ttcacacaag cggggtcaga agtgtcagcg
     5461 cttttaggtc gtatgccttc agccgttggt tatcaaccaa cacttgctac agaaatggga
     5521 caattacaag agcgtatcac ttcaactaaa aaaggttctg ttacatcaat ccaagctatt
     5581 tatgtgccag cagatgacta tactgaccct gcgccagcta cagcctttgc tcacttagat
     5641 tcaacaacta accttgaacg taaattgaca caaatgggga tttatccagc cgttgaccca
     5701 ttagcttcaa gttctcgtgc actcacacca gaaatcgttg gagatgagca ctatgaagtt
     5761 gcaacagaag tacagcgtgt tcttcaacgt taccgtgagt tacaagatat cattgcgatt
     5821 ttaggtatgg atgaattatc agatgaggaa aaaacgttag ttggacgtgc acgtcgtatc
     5881 caattcttcc tttctcaaaa cttcaatgtt gcagaaacat ttactggtca accaggttca
     5941 tatgttccgg ttgaagaaac tgttcgcgga ttcaaagaaa tcctagatgg taaacatgat
     6001 caaattccag aagatgcctt ccgtatggtt ggtggtattg aggatgttat cgcaaaagca
     6061 gaaaaaatga actattaatg aggtgatgct atggcacaat tgactgttca agtagtaaca
     6121 tcagatggta ttagatatga ccatcacgct agtctcatta ctgttagaac gccagatggt
     6181 gaaatgggaa ttttaccagg tcacataaat ttgattgctc cccttattgt tcatcagatg
     6241 aagattaatc gttctcatca agaaggtgta gactgggtag ctgttaatgg tggtataatt
     6301 gaagttaatg aggatcaggt gacaattgtt gcggattcag cagaacgagc acgtgatata
     6361 gaccttaacc gtgcagaacg ggctaaggaa cgcgctgagc gtgcattaga aaaagctcaa
     6421 acaactcaga atattgatga gatgcgtaga gctgaggttg ctcttcgtcg cgcaattaac
     6481 cgtattagcg tcggtaaaaa gtagaaaaat ggtctaatta gttttgtgac taattaggct
     6541 tttttagtgt ataatagaag gcatgaatat gatattaaat atagtaacta tagttagtaa
     6601 ttttattttt attgcccttt tttatcaact ttttgtagat ttatttgact ggtctaaaat
     6661 gattaaaatg tcccctcaaa acatctcaaa attaaaagt
//
LOCUS       DATROY010000100         6687 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.101, whole genome shotgun sequence.
ACCESSION   DATROY010000100 DATROY010000000
VERSION     DATROY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            79..606
                     /locus_tag="VBD11_001564"
     CDS             79..606
                     /locus_tag="VBD11_001564"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6954504.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            597..1274
                     /locus_tag="VBD11_001565"
     CDS             597..1274
                     /locus_tag="VBD11_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6954505.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            1406..2449
                     /locus_tag="VBD11_001566"
     CDS             1406..2449
                     /locus_tag="VBD11_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6954506.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(2540..3439)
                     /gene="galU"
                     /locus_tag="VBD11_001567"
     CDS             complement(2540..3439)
                     /gene="galU"
                     /locus_tag="VBD11_001567"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6954507.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(3476..4492)
                     /locus_tag="VBD11_001568"
     CDS             complement(3476..4492)
                     /locus_tag="VBD11_001568"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6954508.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            4662..4991
                     /gene="rnpA"
                     /locus_tag="VBD11_001569"
     CDS             4662..4991
                     /gene="rnpA"
                     /locus_tag="VBD11_001569"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6954509.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            5004..5819
                     /locus_tag="VBD11_001570"
     CDS             5004..5819
                     /locus_tag="VBD11_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6954510.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            5827..6648
                     /locus_tag="VBD11_001571"
     CDS             5827..6648
                     /locus_tag="VBD11_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6954511.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         2222 a         1041 c         1266 g         2158 t
ORIGIN      
        1 aaaattcata ctttatcatt ttattttccc ctatactatt tttgtataaa tggtcaatat
       61 taatggtatc atagatatat ggaaaagaaa ttattaagaa aagaagtttt gataacctta
      121 aaatctcaac ctcaagctta caaatcagaa gttgactcta aacttttaga ggcttttata
      181 aaaactaaag cttatcaaaa tagttgtgta attgctactt acctatcttt tgactacgaa
      241 tacaatacac aacttcttat taaacaagct ttatgtgatg gcaaacgtgt ccttgttccc
      301 aaaacttatc caaaaggtaa aatgattttt gtggactatc aaaaagataa cttgagggca
      361 acaccgtttg gcttattaga acctgttaat gatagagctg ttgaaaaagc tagtatagac
      421 ttaattcatg tgcccggact tatctttaac aataaaggtt ttcgtatagg ttatggtgca
      481 ggttattttg atcgctattt gagtgatttt gaaggtgata ctattagtac tatttatagg
      541 tgtcagagac aggattttgt agaagagaag catgatgtag cggttaagga ggtattatgt
      601 ctatgaagaa gttcgctaaa gagtatccga cgacagtact cttggtcggt ctgacaaccc
      661 ttgttttttt attgatgcaa ctaacttatg gaagtcaagc agagtctagc caagttattt
      721 ttcaatttgg tggaatacaa ggtgattatc taaaagcata tccaacaaat ctatggagac
      781 taataagtcc tatctttgtt catattggtt gggaacattt tttattaaat ggtttagctc
      841 tgtattttgt gggacaaatg ggggaaagta tttggggaag tttgagattt ctaattctct
      901 atattttatc tggcttaatg ggtaatattt ttacgctatt ttttactcca cacgtggtgg
      961 cagcaggagc ctcaacgtct ttatttggtg ttttttctgc tatagcaatc gcaggttatt
     1021 ttggaaaaaa tccttattta aaacaagttg gaaaatcata tcaagtgatg atcctgttaa
     1081 atttattttt taatatcttc acacccggag tgagtttagc aggccatgtt ggtggtttag
     1141 ttggtggagt attggtggct atttttttga caaaacaaaa tggttcttta ttatttaaaa
     1201 cttggcaaag tattttagct ttaatgatat ttattattgt atcgatcagt ttaataggtc
     1261 tatcgttggt ataattctac ggctatagtc tctaaaacca aactttcttt gggaaaaata
     1321 gaataaagtt tgctaaatga tttttttatg ttatgataga aatctgtttt tatgtgacta
     1381 taacttatta ggaggttcat tctcggtgaa cagaaaaatt attggcttaa ctctactatc
     1441 tttatctgta ctaacattaa cagcatgtgg taatcgttca gataagtctg caaataaatc
     1501 agatatcaaa gttgcgatgg taactaatca aggtggagta gacgataaat cgtttaatca
     1561 atcagcttgg gaagggctac aaaagtgggg aaaaaagaag gggttaacaa aaggtaatgg
     1621 ctttgattat ttccagtcat ctaatgaatc agatcatgct aataaccttg atacggcagc
     1681 ctctagtggc tataatttaa tttttggtat tggatttggt attcatgata ctattgaaaa
     1741 agtatcagaa aataataaag atgttaaata cgttattgta gatgatatca ttaaaggtaa
     1801 agaaaatgta gctagtgtta cttttgcaga caatgaagct gcttacttag ctggagttgc
     1861 tgcagctaag acaactaaga caaaaacagt tggttttatc ggagggatgg aaggcgtcgt
     1921 tgttaagcga tttgaagcag gatttaaggc tggagtaaaa tcaattgatc cggctattaa
     1981 agttgcggta tcttatgcag gctcatttac agatgcagct aaaggtaaaa cgattgctgc
     2041 cacacagtat gcaacaggtg ttgatgttat ttaccaggct gctggtggta caggagcagg
     2101 aatttttagt gaggctaaaa ctgaaaatga gacccgcaag gaatctaaca aagtctgggt
     2161 aattggtgtt gacagagatc aaagtcaaga aggcaattat gtttcaaaag atggtaagaa
     2221 agcaaacttt gttctggcat ctactataaa agaggtgggg aaatctctcc aatctgtagc
     2281 agaattaaca gagaaaaaac aatatccagg agggaaagta acagtttttg gtctaaagga
     2341 tagtggtgta gatattaaag agcatcaatt gtctagtgag ggatctgttg ctgtcaaaaa
     2401 agctaaagag gggattgttt cagggaaaat acaagttcct atgaaatgat aaaagtgtgg
     2461 aggaacaata agttcttcgt caagtacaat aaaaaagttt cctaaatagg aaactttttt
     2521 attgtacttg actcatattt tatttcttag atgttttttc tagagatttt cctaaatcaa
     2581 tgatatattt cttcaagtca tctttaactt gtgggtgttg aagagcataa tcaatagaag
     2641 ttttcataaa accaaacttg tcaccaacgt cataacggtc ccctgtaaat tttctagcaa
     2701 aaacacgttg cgttttattt aaagtatcga tagcatcggt tagttgaatc tcatttcctg
     2761 caccaggttt ttgagtttct aaaatattaa aaatttctgg agttaacaag tagcgaccaa
     2821 tgattgccaa attacttgga gcctcttcgg gagaaggttt ttcaacgaaa gtgttaacgc
     2881 tatatagacc attaactcct tctccttgtg gagcaataac accataagca gaaacatctt
     2941 catgtggaac ttccataaca gcaattgttg atgcatgagt agcttcaaag tcattaatta
     3001 attgtttggt gagaggaata actttattat tggtaatatc cattagatca tcacctagca
     3061 taacaacaaa aggttcatta ccaacgaagg cttttgcttg tagcacagca tctccaagcc
     3121 cacgaggatg actttgtcga ataaaatgga gtcgaatgcc agttgtttca tcaacaagtt
     3181 tgagaagttc gtttttccct ttctctttta ggttatattc taattcaaaa tttgaatcaa
     3241 agtggtcttc tatagaacgt tttgatttac cagttacaac taaaatatct tcaattccag
     3301 attttagtgc ttcttcaaca ataaattgaa tagttggttt atcaacgatt ggaagcattt
     3361 ctttagctaa agctttagta gcaggtaaaa aacgtgttcc taaaccagcg gcagggataa
     3421 ctgcttttct gactttcata tatagctcct ttaaatagta ttaatataaa cgatattagt
     3481 gccattcatt ctctgaacga aattcattgg acatcatatc taaaatgcta tctttgatat
     3541 tagccccttc atatattgat ttataaatag cttctgtaat gggcatgtaa acatttaagt
     3601 tttgggcgat ttcgtaagca accttagttg ttgagatacc ttcaataacc atccccatat
     3661 ttttttcaat atcttcaagt ttctcaccac gtcctaaagc atctcccgct ctccagttgc
     3721 gagaatgaac agatgtacca gtcacaatta aatctccaac tccggataaa ccactgaaag
     3781 tgagagggtc ggcccctaat tgtacaccaa ggcgtgttat ctcagctaaa ccacgcgtaa
     3841 taatagctgc tttagcatta tcaccataac caagaccgtg tagtgctcca gcaccaacag
     3901 caataatatt ttttagagca ccagctgttt ctactcctac gacatcagtg ttagtataaa
     3961 gtctaaaata atgattacta aacaattttt ggacatactt agctgcttca atatcttttg
     4021 aagcagcagt aatcaaagta atatcacgaa cgatggcttc ttcagcatga ctgggacctg
     4081 aaacaacaac gatatcacta cgatattgtt cagagatttc ttcttctaaa attgtagaaa
     4141 gtcgttcatg agtgccaggt tctagcccct tagaagcatg cataattaca actttatgct
     4201 ttaaaagact agccacttgt ttagcaacaa gtcttgtaac tttagttggg acgacgaata
     4261 gaatgctgtc aacattatta atcgcttcct caagattagt gtaggctttt attttggaat
     4321 ctaaggtaat atctttgaag taacgttgat tagtatggtt agtattaatc tcttctattt
     4381 gttcaaccac gtttccccat aagcgtacct catgtccatt gtcatttaat acttgtgcaa
     4441 gagcagtccc ccatgaacca ggtccaagaa cggcaatttt ttgttttgtc atcttattcc
     4501 ttctttctaa taataaggca tatagcgcta acattatatc atactctaat acactttgtc
     4561 ttgtttagtc tgttgtcgat gatagtagca ttagaaaaga attgagtctt ttaggttatt
     4621 tatggtataa taaaaccaat ttagataaat ggagcactat tttgaaaaaa acctatcggg
     4681 taaaaagtga taaggatttt caaatgattt tcagcagagg taaaaatgtt gctaatcgaa
     4741 aatttgttat ctactattta gaaaaagaac aaaaacactt tcgtgtggga atctctgtta
     4801 gtaagaaatt aggaaatgct gttgttcgta acgctgttaa aagaaaaatt aggcatgttt
     4861 tactctctca gaaaacagcg ctgcaagatt atgattttgt cgtgattgcg cgtaaaggtg
     4921 tagaggaatt agactatcaa gcattagaaa aaaatcttat acacgtttta aaaatagcag
     4981 gactcatcta gaaaggtata aaattgaaga aaaaacttaa aacatttagc ttaatattgc
     5041 tgacaggttc tttattggtt gcttgcggac gaggagaagt atctagtcat tcagctacat
     5101 tatgggagca aatagtttat gcatttgcta aatcaataca atggctctcc tttaaccatt
     5161 caattggttt gggaataatc ttattcactc taataatcag agcgattatg atgccgcttt
     5221 ataatatgca aatgaaatcc agccaaaaga tgcaggaaat tcagccaagg ctcaaggaat
     5281 tacaaaaaaa atatccaggg aaagatcctg atagccgttt aaagctaaat gatgaaatgc
     5341 aatctatgta taaagctgag ggagttaacc catatgcaag tgtattacct cttctaattc
     5401 agttacctgt cctatgggct cttttccaag ctttgactag ggtttctttc ttaaaagtag
     5461 gaacgttttt gagcctagaa ttatcacagc cggacccata ttatatttta cctgtactgg
     5521 cggccttatt taccttttta tcaacttggt taactaacaa agcagctgtt gaaaaaaata
     5581 ttgctttaac tcttatgact tatgttatgc cgtttatcat attggtaaca agttttaact
     5641 ttgctagtgg tgttgtttta tactggacgg tgtctaatgc ttttcaagta tttcaaattc
     5701 tattattgaa caacccatac aagattataa aggttcgaga agaagcagta agagtggcac
     5761 acgaaaaaga acaaagagtt aaacgtgcta agagaaaagc aagtaagaaa agaaagtagg
     5821 aaaaacatgg tattatttac aggagcgact gtagaagaag ctattgaaaa aggactacaa
     5881 gagttaaaca tttcacgatt gagggcacat ataaaagttg tatcacgtga gaaaaaaggt
     5941 tttttgggct ttggtaaaaa gccagcaaaa gttgaaatag aaggtatcac cgacgaagta
     6001 actgatatta atgaatcggt agcgttaaaa aatattaaga atgtaccgtc tagcgtagat
     6061 gtggtggaag aatatataga ggaggttgat gaaacgcttg aaaaagaaga cgtatcacaa
     6121 ccagaacttc caaaaattga tgataaaaac gttgtaacta caagtgaagc tattgaaaaa
     6181 attgacctac ttcctaatat tgaagtagca gctgctcagg tgactaaata cgttgaaaat
     6241 atcatctatg agatggattt agatgcaaca atagaaacta cgacaagtaa gcgacaaata
     6301 aatctccaaa tagaaacgcc agaagctggg cgcatcattg gttatcatgg gaaagtatta
     6361 aaatcgttgc agttgttagc acaaaattat cttcatgacc gcttttctaa gagtttttca
     6421 gtatctatta atgtccatga ctacgttgag catcgtatag aaactttaat tgatttttct
     6481 aaaaaaatag ctagacgtgt tttagagact aatgagcctt accatatgga tcctatgagt
     6541 aacagtgagc gaaaaactgt tcataaaaca attgcaacta tagagggtgt tgaaagttat
     6601 tctgaaggta acgatcctaa tagatttgta gtagttacta agaagtaaaa agaggtttca
     6661 cctctttttt tcttgcgccc gatctac
//
LOCUS       DATROY010000101         6629 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.102, whole genome shotgun sequence.
ACCESSION   DATROY010000101 DATROY010000000
VERSION     DATROY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1003
                     /locus_tag="VBD11_001572"
     CDS             <1..1003
                     /locus_tag="VBD11_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6954512.1"
                     /translation="TFHKKDDIDVWVGGKSYNPARSFRTRTINELTVIDFEEMFDILW
                     LMLGDNLIKSFEVNVCGILFELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLIK
                     KLKENPLFNIGFMVNDILERMYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQIDG
                     VKFTQIYEDYMKLLSQGKDIAELYQKYSKEELANLGINIYQSNDIERTEERTFDEIIS
                     WVSNPYATRPIQERHTIQLEPTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLDN
                     TSVNRHIVGLLTNNTQVTYYFQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL"
     gene            1324..1773
                     /locus_tag="VBD11_001573"
     CDS             1324..1773
                     /locus_tag="VBD11_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6954513.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            1975..2295
                     /locus_tag="VBD11_001574"
     CDS             1975..2295
                     /locus_tag="VBD11_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954514.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2295..3395
                     /locus_tag="VBD11_001575"
     CDS             2295..3395
                     /locus_tag="VBD11_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6954515.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3392..3997
                     /locus_tag="VBD11_001576"
     CDS             3392..3997
                     /locus_tag="VBD11_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954516.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            4203..4808
                     /locus_tag="VBD11_001577"
     CDS             4203..4808
                     /locus_tag="VBD11_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6954517.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            4801..5049
                     /locus_tag="VBD11_001578"
     CDS             4801..5049
                     /locus_tag="VBD11_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6954518.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            5139..6404
                     /locus_tag="VBD11_001579"
     CDS             5139..6404
                     /locus_tag="VBD11_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6954519.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT         2344 a         1226 c         1127 g         1932 t
ORIGIN      
        1 tacttttcac aagaaagatg acattgacgt atgggttggt ggaaaaagtt ataaccccgc
       61 tagaagtttt agaacccgta ccatcaatga attgactgtc attgattttg aagaaatgtt
      121 tgatattctt tggttgatgt taggggataa tttaattaaa tcatttgaag ttaatgtctg
      181 tggtatccta tttgaattag gcggaaatga cataccctca acatttaggc aagaaaacat
      241 tgatcctctc atcaataaat ggtggtatga taatgtttcg acagaaatta tccctaatct
      301 aataaaaaaa ctcaaagaaa atcccttgtt taatatcgga tttatggtaa atgatatatt
      361 agaaagaatg tataaagaga atattccaaa atcttacctt acatccgtcc cattagttat
      421 ttctcaaaaa ggaagaacaa cctattcgtt tagtatgact ggtggtcaac aaatagatgg
      481 agtgaaattc acacagatat atgaggacta tatgaaatta ctcagtcaag gtaaggatat
      541 cgcagagtta tatcaaaaat attctaaaga agagttggca aatctaggca ttaatattta
      601 tcaatccaat gatatagaaa ggactgagga aagaactttt gatgaaatta tcagttgggt
      661 ttccaaccct tatgcaacaa gaccaattca agaaaggcac actattcaat tagagccaac
      721 aagattttca ctagaggata agaaaagaat tgaagaagct gcagctcaag gactaagcga
      781 aatcgacctt attgatttag ttgacctata tgatattaat ttagacaata caagcgtcaa
      841 tcgccatatt gtggggttat tgactaataa cacccaagta acatactatt tccaagaaca
      901 attaaataag gagttgctgt caatggctca cgctttagat aacgtacaac aggcctttat
      961 taaattatta agtgaagagg agatacgaaa atttgctctt taaagactag gtgaatcgcc
     1021 tagtcttttt ttgcgcctcg ttttacaaat tagtctattg aagtaaaaaa agaagtaaaa
     1081 aataaaagaa cattacgcct tatactgaat atatcaagaa aaactcttga tataaatttt
     1141 aatagtccaa attgatggcc atcaaaggac agattgaaac aaatatgatg acatgacgat
     1201 aaaggtcatg tcttgcatag aattgtttcg ctgtttcttt gttgtgcctt ttggtatact
     1261 cagcgttcaa tatttgctca ttttgtttcc ttctgtccaa aggacagaaa ggagctcatc
     1321 attatgagta ataaagtaca agaacgtcgt gagcgtaaaa tcaaagaagc tattaaagct
     1381 aaaaattggg atgaagtaac tcgtttgctt caacaagaac aaagtaacgc agaacgtcgt
     1441 gaccgatacc acaatagacg aatcaaagac gaaacaatag ctagcaaaaa tgccaaaaag
     1501 tctgtacgtt atgatgtcat tgcaagttct gatttgaatc ccgaagaagc tttaatccta
     1561 gaggagttaa gacaagctat tcgtgaagct aaagcatccc tatcagaaat tgacagtaag
     1621 attgttgaaa tgatagcgga acaaggttca agctataaag aaacggctcg ctatatcact
     1681 gaacattata agaaaatgag tgatgtaact gttaaatccc attattgtaa agcgctaaaa
     1741 aaattagctc ctttattaaa agcttaccgc taatccctca aatccagtgt ctctgtcact
     1801 ggattttttg taaaaaaaaa ttaaaaattc aacaaataac tttgcgttta gctgtctcat
     1861 tctccttagt aatgaggagg tggtcagata acctaaaaaa caaagtccag ctcatgaccc
     1921 aaaccgagcc tgtcgcattc acactatctc ttattcataa ggaggatttg ccctatggcg
     1981 tttaaaatga aaaccacaaa aggtggttat acaactacat tggctgataa aattatcagt
     2041 caaaaacaac cgatttactc acttagtacc gaacttgaac cacaacaacg gtttgaagaa
     2101 ggaaaaccaa ccggagaaat cgtagcttat aaagcatggt ttgtacagga agggcaagac
     2161 cctttccaag taaaatttga agaaacgatt gagcttccag cttttcaatc catgattcaa
     2221 tttgacacac tacaagcctg cgaagtaaaa tataacatct acttcaaagc aaatggtatc
     2281 aaggaggttc ggtaatgact tcacaatctc gtgatgtgct gaaccaatct tacctgcaat
     2341 cttacctgct ccagagccta aacatggctc tgggggcttt aatgcaggga gaaaccagct
     2401 acaccaattc ttttaatatt gttattcaag cagacggctt tatctttgtc cctcgtctac
     2461 catgtgctta tatccttgat gatgacttgt acaagaaaat ctttctgatt gccaatgctt
     2521 cactttatcc acaatatacg cttctaaaac aaaacgctac ctattttgtc cctctagaaa
     2581 cagatgactt acacactcag cgtggattat ttttcccttg gaaaaaggga atttcagaac
     2641 gtttagtcat tccagatttg gataagtttt cagctagctt gccacagggg aaaatcccta
     2701 tcatgaagta ctttgaactc aatttagata aggttaatca ctgggctatt gctggaaatt
     2761 caggttctgg gaaatcgtac gctctcactt atttcttgag tgtactaaag catatgtctg
     2821 acttgattat cattgaccct aaatttgata ccccaagtcg atgggcaaga gaaaatcacg
     2881 ttgctgttat acaccctgtt gaaaaccgtt caaaatcaga ttttgtctca caggtcaatg
     2941 aacaattaag tcagtgtgca actctcattc agaaacgaca agctatcttg tatgataatc
     3001 ccaatcatca atttacccat ctaactattg ttattgatga agtcctcgct ctatcagagg
     3061 gagtcaataa gaatatcaaa gaagcctttt tctccctact ctcacagatt tccttattag
     3121 gacgtgctac caaaatccat ctgtttttgg taagccagcg ttttgaccac aatactattc
     3181 caatttcagt gagggaacag ctaaacgtat tgttgcaaat tggaaatatc aatcagaaaa
     3241 ctactcaatt tttatttcca gacctagacc cagagggtat tgttattcca ataggacatg
     3301 ggacaggtat cattcaagtt atcgataatg aacatcccta ccaagttctg cccttactct
     3361 gtccaaccta ctacactaaa cgaggtattc tatgaccaat aaaacctact ttatgagaag
     3421 ttttaatctc atactatatt tatttctatt tgcacctatc tgttctgcat ttggatggtt
     3481 attcaattat ctttccgttc aattaacaaa tatcacgttt gtcaatttag atacggtcaa
     3541 aagtattacg gatatcatta gttccgtttg tcatggattt gcacttattt ctcttctact
     3601 tgccttattt ttggttggat tagaaataat acaacggttg aaaacagata gtctttggaa
     3661 ctatatccaa tctgtctatc acacttttct gttgcgacac ttcctgtttc agcgtgaacg
     3721 agtacagaag ataagcaatc ttgaacatca gacggttact actatcaacc caatccataa
     3781 cggatttaac cgagctgttc gtaagtgtat cgtagatata cgaaaagata ccatcactat
     3841 ttttctaaaa gtccctagag accaacaagg gcaaaaaatc ctaaaagaga tggaagcaca
     3901 attaaaagaa gaaatcgtca gccaacacgc agactattat ttctcctctc ctattcgagt
     3961 tcgtaaccaa ttgtggttca ctggacaaaa acgttaatcc cttggggctg tggcttgcgt
     4021 taagcaaaag ccagacagcc cctttttata ttcttttcta tcatgaccta ttggggtgac
     4081 tacgaccccc aataggtcaa taatcaataa tcacaaaaaa ttcaattttt tcaaaaaaac
     4141 tttgcatacg acctgtcatt tctccttagt aatagaaccc aaaagaaatg aggtaactgc
     4201 ctatggcaaa agaacagcgc tcaaccaaat ggacttttct cttctacgag gaaagcgccc
     4261 cagagaacta cttgaatata ttagaggaac tccatattcc gtttattctt agtccttggc
     4321 acgataagga cgttaataga cagacgggag agttcaagaa atcgcacaaa cacggtgctt
     4381 tcttctttga ttcactgaaa agctattcac aagtatcaaa tatcatcagt gacaaactaa
     4441 acggtccagc acatgtggag gtagttatgt ctcccactgg tttatttgac tactttaccc
     4501 atgcggaaaa tccagataag accccctaca acattgagga tattgaagtc ggttgcggct
     4561 ttaatttaga aaaattcttg atggagatga actcctcgga ttttatacat gaagtagttg
     4621 atattatcga agagaacgac tttaccgaat ttgaagaatt ggtctggtat gcccgagcaa
     4681 acaataccaa tttattaggg cttatcattg aacgtacata tttcttcgct aaatacctag
     4741 attcacgaag atacaatcct aatcgactcc ataattcaaa cacagaggag aaagagaata
     4801 atgaataacg aaaaattaga acaaattgaa caattattgc aaacacttat caaactcatt
     4861 caaatcaaag aacagaacat accactaaag ataatacaac agcgagaatt gctaaaacag
     4921 ctaaatatct ccccaaacac actaaaaact tgggaacaaa aaggattaaa aagattagaa
     4981 cctcctatag agggaactcg aaccgtcttc tatctactag atgatattat caacttttta
     5041 caatcctaat tctgaaaatt tgtgctaaaa tagttttatc taaaagaaag agtggtaact
     5101 attttagcct acttcaatat ccatgaagaa aggcaaatat ggaaactgaa acaattattc
     5161 ataatggcaa aaaagtcatt aaaaaaatca aaaaagataa gtccatctca tacactttga
     5221 aggggacatt tctgggaaaa gatatcaaaa ctggtaagca agtcactact accattacag
     5281 ccaagacact aaaacagtta gatagagcaa tcattcaagc acgactagaa tttgaaaaaa
     5341 atggctcaac acgagaaaaa gttatcgtca ttgatacact ggaagattta gctgaagaat
     5401 ggtttaaatc atataaaacc tgggttaact cgcataatac tctgaacaga gtaagaggtt
     5461 atcttgataa ttatattatc cctaaatttg gagattacaa accagataaa attgaatctg
     5521 cggatatcca gctttggtta aacgatttgg caaagaaatc aaaagagtcc attgaatctg
     5581 gtataaagcg tgctgataaa ggtagtgcta aagattttgg tgctgttatc cataagttga
     5641 aagatatttt tgactatgga attacaaact atggactaat cactaatcct gttagcactg
     5701 ttaagatacc acctaagcca aaatcaaata agcaacgcat catggtatta catgatgatg
     5761 gtcttgtaat atggcttaat tatcttgaaa gtttagataa caatagagcc aatcgtagat
     5821 ttaagttaat ttgtaatact ctattagcct ccgctttgcg aataaatgaa ttacttgcat
     5881 tgacgattga tgacctagat tttgaaaatt cttctattaa cgtcagtaaa acattgatgt
     5941 ggaaaaccgc aaataaaaaa gatgggacaa aaggggaagt tatctgtaaa gctacaccaa
     6001 aaactgatgc aggaaatcgc tctgtccctg tccctccccc cataatagaa aaactcagag
     6061 atttccacca cgaaatgaac aagtatcttg aattgcacaa cagacataga acaaaactga
     6121 ttttccctac aatctatggg aactatatgt gtgacagaaa cgagagaaca actcttaaga
     6181 aaaggttagt aggtcttgga ttaccaaact atggtttcca tttattccga catactcatg
     6241 cttcaatgct acttaatgct ggaacaaatt ggaaagaact ccaagtcaga atgggacata
     6301 aatccatttc aactacaatg gatacttacg ctgaattagc accacaacga aagttagaag
     6361 ctgtggggat ttatcttgaa aagattgctg agctaacgca ataactactc taccattcac
     6421 tctacctttt attatgtagc attctagaaa cattgatgta ccaacgtttc taacagctaa
     6481 aatagaaatt atttaactct tactgaagtt aaataaggat tcattacacg tcaataggca
     6541 gtaaaaagat tggttttatg agtttttgaa attttatatt tcactacact gcaatataaa
     6601 tcagttgaat ctctacattt ttctctaca
//
LOCUS       DATROY010000102         6616 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.103, whole genome shotgun sequence.
ACCESSION   DATROY010000102 DATROY010000000
VERSION     DATROY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..119
                     /locus_tag="VBD11_001580"
     CDS             <1..119
                     /locus_tag="VBD11_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6954520.1"
                     /translation="QSVMFKEDNYIDAYRMYGRLLRKVKLVDKLKLIKNRFF"
     gene            203..1159
                     /locus_tag="VBD11_001581"
     CDS             203..1159
                     /locus_tag="VBD11_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO6954521.1"
                     /translation="MTNFVCHTLYHLLITIIKLKDKENTRIFICDTITDYETWVKILN
                     DQGIRTESFKEFAYREQLQNKNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIGRYM
                     VYLGKNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVNSLV
                     GLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLAQDK
                     CYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPSNVP
                     MEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            1156..2556
                     /locus_tag="VBD11_001582"
     CDS             1156..2556
                     /locus_tag="VBD11_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6954522.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDTIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2556..3581
                     /gene="neuB"
                     /locus_tag="VBD11_001583"
     CDS             2556..3581
                     /gene="neuB"
                     /locus_tag="VBD11_001583"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6954523.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3658..4812
                     /gene="neuC"
                     /locus_tag="VBD11_001584"
     CDS             3658..4812
                     /gene="neuC"
                     /locus_tag="VBD11_001584"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6954524.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4809..5438
                     /locus_tag="VBD11_001585"
     CDS             4809..5438
                     /locus_tag="VBD11_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6954525.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5449..>6616
                     /gene="neuA"
                     /locus_tag="VBD11_001586"
     CDS             5449..>6616
                     /gene="neuA"
                     /locus_tag="VBD11_001586"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6954526.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGL"
BASE COUNT         2176 a          968 c         1235 g         2237 t
ORIGIN      
        1 aacaatctgt aatgtttaaa gaagataact atatcgatgc ttatagaatg tatggacggt
       61 tacttagaaa agtgaagtta gttgataaat taaaacttat aaaaaatagg tttttctaga
      121 aatggctgtt taatagttgt aatgtgagtg accacgaatt gttggataac tatcaaaaag
      181 actaatttag gggaaaaata aaatgactaa ttttgtttgt catactttat accatctact
      241 gattactata ataaagttaa aagataaaga aaatactaga atatttattt gtgacactat
      301 cacggattat gaaacttggg taaagatatt aaatgatcag ggaataagaa ctgaatcatt
      361 taaagagttt gcttatcggg agcagttaca aaataaaaat attgaagaag tgatggagct
      421 tgtagattca gacttaaatc actactttga gagagtagat acacaagtct atctatttaa
      481 tgatgatact ttaataggtc gctatatggt gtatttaggt aaaaattatc atctaattga
      541 agatggttat aactgttttc aagccaagtt attcttgggt ggttctgtcg taaaacgagt
      601 aattaaaact tacttattta aaaagtatgt tccgtatggt ttttcaaaat attgtctctc
      661 aattgaggtt aattctttag tggggttacc tcatgacata aggagtaaga aatataaaga
      721 actaccgaga aaaaaattat ttgatagtct taacaaagaa caaaaatcac tgattttcaa
      781 aatatttaaa acaaaaccat taactataac tccaaagtca gtattattgt tgacacagcc
      841 acttgcacaa gataaatgtt ataaaacacc tacagagagg tttcaaagta ttcaagagca
      901 atacgattat tttgacgata ttgtccagga atatagaacg ttagggtaca atgtttattt
      961 aaaagttcat cctagagatg tagtagatta ttccaaattg ccggtagagc tattaccatc
     1021 aaatgttcct atggaaatta tagagttgat gtcaacaggt cggttcgaat gtgggataac
     1081 acattcgtcc actgcgctgg attttttaac ttgtgttgat aaaaaaataa ctttagtaga
     1141 tctgaaggat attaaatgaa tactaagaaa ttacttcaat ctggttttat ttatacactc
     1201 gggaatttat tggtgcaagg attagccttt atcacacttc ccatctatac tcgggttatc
     1261 tcaacagaag tttatggcca atatagttta tatgtcgctt ggatgaatat tattatgctt
     1321 tttattggcc ttcagacgag cggttcttta agttcagccc gggtcaaata cggagaagaa
     1381 tttaaaagct attcagggag tgccttttct gtaggtaata tatggtttct tattatactt
     1441 ttgatagctt ttctatttag aagttttctt gcaccattag ttggtttttc tgaatctatt
     1501 tttttattaa tggtgtgtca aagttacgct agctatgtgg tgactttctt tggtcagtat
     1561 tttatacaac aacagaggag tttggctaat ttaatattat ccttagccaa tgcagtttca
     1621 tctgttgcac tatctctatt tttaattttt cattggtccg atgacttttt atctagggtt
     1681 tttggagctt ttgttcctac tataataact ggaatagttg cctttgctta tatttattat
     1741 catagcaaat ctttttacaa tcctaagtat tttcggttca ttgtcactgt gtctgttccc
     1801 ttgattttcc atttgttagg acaccagttg ttggggcaat tagatagaat tatgctagct
     1861 cgtttataca atactaaaga agttgcaatg tatagtttcg gttattcgct tggaatgatt
     1921 attcagattg ttttgaatag tattaatatg gcttggattc catggttttt tgatgctaga
     1981 aaagaaaaat tactgcaact ttcaacttat attagccgtt atctctactt aggagttttc
     2041 ttaacattag gttatttgac tgtttttcct gagttagctc aaataatggg aggagacaag
     2101 tatagtagta gtgttcaatt tatttcttta attattgtca gctacttttt agtttttctc
     2161 tatacatttc ctgtaaatat tcaattcttc tatgctaata cgacatggat tcctattggg
     2221 acattattag ctgctggtgt aaattggtta ctaaaccttg tattaattcc tcattatgca
     2281 gcctatggcg ctgctatggc tacaataata tcttatttag ccttgttgat ttttcatcat
     2341 attgtttcga aagttaaata ccattattca gatgtctcag ttaggcagta catcatttta
     2401 agtggtatag tatttagtta tgctatgtta atgaacatgt tcctagatac tatcgttatt
     2461 agatggagtt taggaataat tattcttata gtatatagca ttgtttttca aaaagtcatt
     2521 ttagacttat tgagtaaaaa aagaaggaga agataatggt ttatattatt gcagagattg
     2581 gttgcaatca taatggagat attaatcttg cgaaaaaaat ggtagatgtt gccgtgtctt
     2641 gtggtgttga tgctgttaaa tttcagactt ttaaagctga gaaacttatt tctaaatttg
     2701 ctcccaaagc tgaatatcaa aaagcaacta caggaacagc agacagtcaa cttgagatga
     2761 cgaaacgttt agagttaagc tttgaagaat acttagaaat gcgtgattat gcaatttcaa
     2821 aaggtgtgga gaccttttca acaccttttg atgaagagtc attagagttc ttaatttcta
     2881 cagatatgcc aatttacaaa attccatcag gagaaatcac taatttacct tacttagaaa
     2941 agattggcaa gcaacaaaag aaagttattc tttcgacggg tatggcggta atggaagaga
     3001 tccatcaagc ggtgaatatt ttacgtcaga atggtacaac cgacatttct attttacatt
     3061 gtacaacaga gtacccaaca ccttacccct ctctaaattt aaacgttatt catactttga
     3121 aagatgaatt taaagattta acgataggtt attcggatca ttcaattgga tcagaagtac
     3181 ctatcgcagc agcagcaatg ggtgcagaag ttattgaaaa acactttact ttagatacta
     3241 atatggaagg tccggatcat aaagccagtg caacacctga tattttagct gctttagtta
     3301 aaggggttcg cattgttgaa caagccttag gtagatttga aaaaatccca gatccagtag
     3361 aagagaaaaa taagattgtt gctcgtaaat cagtcgttgc tttaaaacca attaaaaaag
     3421 gcgatattta ttcaatagaa aatattacgg tgaagcgccc aggtaatggt atttctccta
     3481 tgaactggta tgacatcttg ggacaagaag cgcaagatga tttcgaagag gatgaagtta
     3541 ttcgtgattc acgctttgaa aatcaattgc ccgagttata ataacaccta attataatcg
     3601 gcaacagatg tattacacgg ctaagattat gtaattacta tatatagaag ggatacgatg
     3661 aaaaaaattt gtttagtaac cggatcgcgt gctgaatacg gaataatgaa gccattgatt
     3721 caaagattat caaaagataa agaagtcaac ttacaaatta ttgcaacagc aatgcatctg
     3781 gaagaaaagt acggctatac ttatcgtcaa attgaagaag acggttttga tattgcttat
     3841 aaagttccct tacatcttta tgatactgac agaagaactg tatctactgc aatggcgcat
     3901 ttacaactag gattgaccaa aatttttgac aaggaagact atgatctagt catcatttta
     3961 ggggatcgtt acgaaatgtt accagttgtg aatgtagcgt tgatttataa tgtcccagta
     4021 tgccaccttc atggagggga gacctcatta ggcaattttg atgagtatat tcgccatgca
     4081 attactaaga tgagtcacct acacttagtc tctacagagg actttcgtca acgtgtgatt
     4141 cagatgggag aacaacctca atttgtaatt aacacaggag ctctcggagt ggaaaatgct
     4201 ctatcaatcc cttctctaac caaagaagca atagaaaagc aattaggtat tgtactagag
     4261 gaatcctatt ttgttgttct ctatcatcct gttacttttg aacaaggaaa aagtgctgga
     4321 gaacagatga aagcggtact atcagccctt tctaaatttg gtgtccagtg tctatttata
     4381 ggatccaatt cagatactgg ttctgatgat attgcaaagg ctatcaatac ttatttgata
     4441 aatcatgaaa atagttattg ctttgcttct ttatcaaccc aactttatca ttccttaatt
     4501 cgtcattcac taggactgat tggtaattcg tcttctggtt taatagaagt tccttctttg
     4561 atgaaaccta ctcttaatat tggtgatcgc caaaaaggta ggctacatgg agaaagtgta
     4621 gtaagtgtac cagtcgaaac cccctctgta ttggagggat tatcaaaatt gaatgaagtg
     4681 accaactttg acaatcccta ctacaaagag aatgcttcat ctattgcata tgaagcaata
     4741 aagctttatt tgaaagatga accttcaatt tcccaaccat tttatgattt aaaggagaat
     4801 aatctaaaat gaaaaaaaag ttggcatttt taggcgcagg aagtcatgct gatgcgattg
     4861 ctccggttat tgatcctctt gtgtatgatt tcgtaggttt ttttgatgat aaagatatta
     4921 cggagcatga tggttatcct gttcttggaa aactttatga tgtgctacct taccttgaag
     4981 atggctcaat agatgcagta tttattacaa taggtgataa tgctaaaagg aaagaactat
     5041 ttgaatatgt agcaaaggat tattatgact ttattattaa catcattagt cccaatgctt
     5101 tagtattgac accagatagt atttgtgggc gtggtatctt tattggtttt ggggctttta
     5161 taggttctaa agtgaagctg tttgataaca acgttgttaa tacaggagcg ctcattgaac
     5221 atcatactgt tgtagaatca cactgtaata tagcacctaa tgctaccata aatggtcttt
     5281 gttatattag agaagaagtt tatgtaggta gtgccagtgt tattattcaa accttggata
     5341 tttcatcgtg tacgacaatt ggcgcaggag ctgttgttgt aaaagatatt atagaacctg
     5401 gaacctatgt aggggttcca gccaaaaaaa taaaataaag gatatgttat gaagccaatt
     5461 tgtattattc ctgcgcgatc agggtcaaaa ggattaccag ataagaatat gttattccta
     5521 gcaggtaaac ccatgatttt ccatactata gatgctgcaa ttgaatctgg aatgtttgat
     5581 aagaaagata tttttgttag tacagattca gaattgtaca gagagatttg tttagaacgc
     5641 ggtatttcag tggtgatgag aaaaccggaa ctttcaactg atcaggcaac ttcgtatgat
     5701 atgttaaaag attttttatc tgactatgaa gataatcagg agtttgtgtt acttcaagta
     5761 acctctccac taagaaaatc atggcatata aaggaagcaa tggagtatta ttcttcacat
     5821 gatgttgaca atgttgtaag tttttctgaa gttgagaaac accctggtct gtttacgaca
     5881 ttgtctgata aaggctatgc tatagatatg gtgggagcag ataaaggtta tcgtcgccaa
     5941 gatttacaac ctttatacta tccgaacggc gctattttta tttctaataa agaaacttac
     6001 ttaagggaaa aaagcttttt cacctctagg acatatgctt atcaaatggc aaaggaattt
     6061 tcattagatg ttgatacgag agatgatttt atccacgtca tcggtcacct ctttttcgat
     6121 tatgccatta gggaaaaaga gaataaagtt ttttataaag aaggctatag tcgtttgttc
     6181 aatagagaag cttcaaagat aattttaggt gattcaaaaa cgatatctat ctcactagaa
     6241 aattaccata attattccca aggtggtgta acattagcaa cgatgttgga aaacttacct
     6301 aactttttga cagctaatgt aacagaggct tttgtttcta taggggtaaa tgatctcatt
     6361 acaggtcata gtgtggaaga aatattcagc aatttccaaa aactctactc attattagct
     6421 gagaataaaa taaagatgag attgacaact attgcttaca ccctttttag agaaactgtc
     6481 aataatgcag atattgagaa aatcaatcaa tggctaacag aattttgtta tcaaaatcag
     6541 attccactgt tagatattaa tagattttta tcaaaggatg gtaatctaaa ttatcattta
     6601 actagtgatg gattac
//
LOCUS       DATROY010000103         6575 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.104, whole genome shotgun sequence.
ACCESSION   DATROY010000103 DATROY010000000
VERSION     DATROY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1621
                     /locus_tag="VBD11_001587"
     CDS             <1..1621
                     /locus_tag="VBD11_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6954527.1"
                     /translation="KPSLALSKLNGFKEYDKTYEDILHCRSLRSHLPKILNTFRISNY
                     YGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLPYYLKMSLFQMEI
                     AKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFANYYYIQATVETL
                     YGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKRKILEDSFLIFSQ
                     PLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQKAKEMFPKLLKQ
                     TFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYYLPNEDKMISYSP
                     PGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQPIWDSNYQASAT
                     DLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKNEIPRDSELYSIS
                     FDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILISAKNGIFYSYKE
                     NNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            1618..2571
                     /locus_tag="VBD11_001588"
     CDS             1618..2571
                     /locus_tag="VBD11_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6954528.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            2568..4763
                     /locus_tag="VBD11_001589"
     CDS             2568..4763
                     /locus_tag="VBD11_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6954529.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            4768..5979
                     /locus_tag="VBD11_001590"
     CDS             4768..5979
                     /locus_tag="VBD11_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6954530.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            5948..6523
                     /locus_tag="VBD11_001591"
     CDS             5948..6523
                     /locus_tag="VBD11_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6954531.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
BASE COUNT         2261 a          967 c         1248 g         2099 t
ORIGIN      
        1 taaaccctcc ttagctttat caaaattgaa tggcttcaaa gagtatgata agacatatga
       61 agacatactg cattgtcgat cattgcgtag tcacctccct aagatattga atacgtttcg
      121 tatttcgaat tattatggta aaacatacga catatcaact tttcaaatta tggaggaaat
      181 tgaaaatccc ttggaagagg agacagaaat taggaccaaa gtctctgatg aagaaatatt
      241 atttaaagca gaagatagtg cttcacttcc ttattattta aaaatgagct tgtttcaaat
      301 ggaaattgct aagttagata accgttatgt tttacaagtt gactttttat cggaacaagt
      361 aaagagggtt cgggttaaaa ctggaaaata tcacttggca aacttaacga gggctcaccc
      421 tagcctaact ttaagtcgtt ttgccaatta ttattacatt caagcaactg tggaaaccct
      481 ttatggtaat atcgatgttc aactggaacg tcgaaaaaag cattatagag atgcaactat
      541 ttgtcttaag agaacttttc aaggttatga tttacttatc tctccaaatg gcagccttat
      601 ttttgaaaag cagaaaagaa agatacttga agatagtttt ttgattttta gccaaccttt
      661 ggacaagaaa ttagttgtaa aagaagaaga aaatcacatt acaatcaaat gtttctatca
      721 aggtgcttta attgagaaaa tcatgacatt tacaagtgac gaagaaatca cttgtgttta
      781 taaatgtaat caaaaggcta aagagatgtt cccaaaatta ttgaaacaga ctttcaagtt
      841 acactgccaa gagcagttga ttagagatag tgaaggttat cttgtgaatg ttccaggaag
      901 ttacccgatt gagcatgatg attttcttcg agcagataag tttgaagata gacaattcca
      961 ttattacctc ccgaatgaag ataaaatgat ttcctattct cctcctggca aagccagcaa
     1021 tcaaatgcaa tttagaccct tatgtcttat tgatacagat agcctttctt ttccactgac
     1081 ttatcatttt aggatttcac aagcatcatc ggaggaggcg ttaatcaatc taaaaaaaca
     1141 gccaatatgg gacagcaact accaagcgtc agcaactgac cttttaaaac atatctctaa
     1201 cctaacatta gaagaagaga aggattatgg catcaagaga atgatagcta acagaaagca
     1261 ttatcctagt cacaaactgg ttttagccta taatcagatt gttttgccta agaatgaaat
     1321 tccaagagac agtgaattat attctatttc ttttgattat cgaattagag gaaagtttgt
     1381 ccaaatccgc caaggggaac atgtcaaata tgataataaa tcctatgttt tggaaaatag
     1441 tcaacaatta gtattgtatg tagctagcga tgataaatat atactaattt ctgctaaaaa
     1501 tggaatattt tattcttaca aagagaacaa tcatttaaaa ataaggtgca tgtttaaaag
     1561 gaactcacct tatgctacaa atgtgagcat tacagaatat agaaagtgtg aagaaaaatg
     1621 aatactattt ataatacatt gagaacagat aaaggttata aagtttatga ggggtattta
     1681 tatgaaatta ctggtgaaga atgtgaagaa gccttagacc ttgtgattcc taagaatatt
     1741 gtatttgcag atacagatac ttgtggctac acttttttac tcaatgaaga tggaacagtt
     1801 tatgatgatg tgactttcta caaatttgat gataaatatt ggttggctag tcataaagct
     1861 ttggattctt atttagacaa catcaatttt gactataccg taacagatat ttctgacgag
     1921 tataaaatgc tgcaaattga aggaagatat tcgggagaaa ttgctcagtc attttatgaa
     1981 tatgatattt caacacttaa ttttcgtact ttgatagaga tgacttataa aggtgagaaa
     2041 ggttatcttg ctagatttgg tttttctgga gaatttggct atcaattttt cctaccatct
     2101 tctatttttg ctacttttgt ttcggatgtc tgtgaaggta tagcagagta tggggatgaa
     2161 cttgatagat atttaaggtt tgaagtggga caacccatta ctgatattta tcaacaagaa
     2221 gaatattctt tatatgaaat aggttattct tggaatctag atttcacaaa ggaagaattt
     2281 agaggtcgcg atagcttgtt agagcacatc agatcagcaa cagttaaaag tgttggattc
     2341 tcaacgaagg aaaaactcgc ttcaggaaca ccagtgctat ttgatgacca aattgttgga
     2401 aagatttttt ggatagcaga cgagaaagac tcttcggaaa attacctagg tttgatgatt
     2461 gttaaccaaa catatgctca ttcaggagtt acttttgtaa cagaagatgg ccaaattttg
     2521 aaaacacaat caagccctta ttgtatccca gaaagttgga acaaagaatg agcgtatatg
     2581 ttagtggaat aggaattatt tcttctttgg gaaagaatta tagcgagcat aaacagcatc
     2641 tcttcgactt aaaagaagga atttctaaac atttatataa aaatcacgac tctattttag
     2701 aatcttatac aggaagcata actagtgacc cagaggttcc tgagcaatac aaagatgaga
     2761 cacgtaattt taaatttgct tttaccgctt ttgaagaggc tcttgcttct tcaggtgtta
     2821 atttaaaagc ttatcataat attgctgtgt gtttagggac ctcacttggg ggaaagagtg
     2881 ctggtcaaaa tgccttgtat caatttgaag aaggagagcg tcaagtagat gctagtttat
     2941 tagaaaaagc atctgtttac catattgctg atgaattgat ggcttatcat gatattgtgg
     3001 gagcttcgta tgttatttca accgcctgtt ctgcaagtaa taatgccgta atattaggaa
     3061 cacaattact tcaagatggc gattgtgatt tagctatttg tggtggctgt gatgagttaa
     3121 gtgatatttc tttagcaggc ttcacatcac taggagctat taatacagaa atggcatgtc
     3181 agccctattc ttctggaaaa ggaatcaatt tgggtgaggg cgctggtttt gttgttcttg
     3241 tcaaagatca gtccttagct aaatatggaa aaattatcgg tggtcttatt acttcagatg
     3301 gttatcatat aacagcacct aagccaacag gtgaaggggc ggcacagatt gcaaagcagc
     3361 tagtgactca agcaggtatt gactacagtg agattgacta tattaacggt cacggtacag
     3421 gtactcaagc taatgataaa atggaaaaaa atatgtatgg taagtttttc ccgacaacga
     3481 cattgatcag cagtaccaag gggcaaacgg gtcatactct aggggctgca ggtattatcg
     3541 aattgattaa ttgtttagcg gcaatagagg aacagactgt accagcaact aaaaatgaga
     3601 ttgggataga aggttttcca gaaaattttg tctatcatca aaagagagaa tacccaataa
     3661 gaaatgcttt aaatttttcg tttgcttttg gtggaaataa tagtggtgtc ttattgtcat
     3721 ctttagattc acctctagaa acattacctg ctagagaaaa tcttaaaatg gctatcttat
     3781 catctgttgc ttccatttct aagaatgaat cactttctat aacctatgaa aaagttgcta
     3841 gtaatttcaa cgactttgaa gcattacgct ttaaaggggc tagaccaccc aaaactgtca
     3901 acccagcaca atttaggaaa atggatgatt tttccaaaat ggttgccgta acaacagctc
     3961 aagcactaat agaaagcaat attaatctaa aaaaacaaga tacttcaaaa gtaggaattg
     4021 tatttacaac actttctgga ccagttgagg ttgttgaagg tattgaaaag caaatcacaa
     4081 cagaaggata tgcacatgtt tctgcttcac gattcccgtt tacagtaatg aatgcagcag
     4141 ctggtatgct ttctatcatt tttaaaataa caggtccttt atctgtcatt tcgacaaata
     4201 gtggagcgct tgatggtata caatatgcca aggaaatgat gcgtaacgat aatctagact
     4261 atgtgattct tgtttctgct aatcagtgga cagacatgag ttttatgtgg tggcaacaat
     4321 taaactatga tagtcaaatg tttgtcggtt ctgattattg ttcagcacaa gtcctctctc
     4381 gtcaagcatt ggataattct cctataatat taggtagtaa acaattaaaa tatagccata
     4441 aaacattcac agatgtgatg actatttttg atgctgcgct tcaaaattta ttatcagact
     4501 taggactaac cataaaagat atcaaaggtt tcgtttggaa tgagcggaag aaggcagtta
     4561 gttcagatta tgatttctta gcgaacttgt ctgagtatta taatatgcca aaccttgctt
     4621 ctggtcagtt tggattttca tctaatggtg ctggtgaaga actggactat actgttaatg
     4681 aaagtataga aaagggctat tatttagtcc tatcttattc gatcttcggt ggtatctctt
     4741 ttgctattat tgaaaaaagg tgataaaatg agtaaatttt tatttgctcc tttcatgatg
     4801 aatttaggag agagccaaag attatcaaaa ctggctaatt gtttatatga acaaggacat
     4861 gatattcata tccttggaga caattattat ccatttttat ttaataatga tgcttatcgc
     4921 tatcaccact gtgtagaaga taaatctgtt tataatagta agcgatatga tgctttcttt
     4981 tctctttcaa cagactttaa ttttttatca gaagaagaaa ttgaacgcat ctgttctatt
     5041 gaaagagact tgttacgcca agaaaaattt gatgctgtat tgacaggtta ccgtctatct
     5101 attgttacaa gttgtcgttt agaaagcata ccattaattt ggattatttc aggggcaacg
     5161 catatcagtg aaattgttga aaactcagaa ggaatattgc ctaatgggaa gataagtaaa
     5221 gctagtaagc ctcaaacaaa ggattttatc aaaagagtaa tcactactta ttcaacaaat
     5281 gttaaaacgt ggaataacta tattaaaaaa tatggtggca aaccttttaa taatgcttta
     5341 gaattattta ctggtgatct aaacttggta acagattact ctttgtttta tgaatttgat
     5401 aaagattcgt cctataaaac gataggacct attttgatag ataatgtagg tttttcaaaa
     5461 tgcagccaaa ttaatcaaga caataagact gtactgctca gctttggtac ttcatttaaa
     5521 cgagattggg tggaatcttt tttaaagaca ttaccaaggc attatcatta tttactgaca
     5581 agctgtggtg aggctataaa cattccagga agttatattg agatagttga ttttattgat
     5641 tttaaaacca tagataaaga gatttatttc gctattatac acggtgggca aggaacagtt
     5701 tatgcgatgg ctgcacaagg tattcccttt ataggtatcc ctttctttaa cgaacaattt
     5761 tggaatatta agaagttcag taaagaaaag tgcgcttatt tggtaaagaa acctgatgta
     5821 agagagatac gtaaagcaat tagctgtctt gaaagagact atgatatcta taaaagttat
     5881 atgataagac taagccaagg aattaaagaa gaagcagagg catctttaca aaaagcggag
     5941 gcagaaattg aatcctttat cagaaaaaga caaagttaac tatattatga tttataaaaa
     6001 taggcagtct acattggaga ataatttatc tggcgatcgg ttgatgagat atcaacaagc
     6061 aaatagacag gtaagcaaag ataaactgtt aacagggagt tattttgtgt accaatgctt
     6121 aaaaaagtta ggttttacca ataagatttg tcaggaatct ttttctagtg ttagtagtta
     6181 tttaattggt ttgccaaagg ggaaaattag ttactctaat tctggtgact atcatattct
     6241 aacctatgct cccagtggtt caactggggt tgatattgag aaatacaaag atagatcaga
     6301 acaaacctac caaaactacc taggagaatc agttagtagt gatatggatt ctaaattatt
     6361 attttataag gcatggttac aaaaagaaat ttcctataaa tgtgggaaat ctatagatat
     6421 tacctatcaa acaatgatag atggctatat ttatggttat gcttttgata atacttttaa
     6481 agtagaagcg gtttacttaa aagaattatt accagataac taactcgcaa catgttgttc
     6541 ctacatatgc atctataaaa agcactttga agtat
//
LOCUS       DATROY010000104         6383 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.105, whole genome shotgun sequence.
ACCESSION   DATROY010000104 DATROY010000000
VERSION     DATROY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..120)
                     /locus_tag="VBD11_001592"
     CDS             complement(<1..120)
                     /locus_tag="VBD11_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6954532.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNS"
     gene            complement(117..785)
                     /locus_tag="VBD11_001593"
     CDS             complement(117..785)
                     /locus_tag="VBD11_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6954533.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(895..2850)
                     /locus_tag="VBD11_001594"
     CDS             complement(895..2850)
                     /locus_tag="VBD11_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954534.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(2852..3604)
                     /locus_tag="VBD11_001595"
     CDS             complement(2852..3604)
                     /locus_tag="VBD11_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954535.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(3630..4592)
                     /locus_tag="VBD11_001596"
     CDS             complement(3630..4592)
                     /locus_tag="VBD11_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO6954536.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            4758..5245
                     /locus_tag="VBD11_001597"
                     /pseudo
     CDS             4758..5245
                     /locus_tag="VBD11_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
     gene            complement(5277..6125)
                     /locus_tag="VBD11_001598"
     CDS             complement(5277..6125)
                     /locus_tag="VBD11_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6954537.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(6258..>6383)
                     /locus_tag="VBD11_001599"
     CDS             complement(6258..>6383)
                     /locus_tag="VBD11_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954538.1"
                     /translation="DASRGFKKVILISEEENRKMYEHQGYEEVAYYWTALRSPRK"
BASE COUNT         2121 a         1116 c          958 g         2188 t
ORIGIN      
        1 ggaattaaac aaatagggca ttggtaaatg atatagatag aaactaataa aaaataggac
       61 aaacatcatg atatataaaa tataccaaat caagtgttct cttaaaaact ggcgtatcat
      121 tgtagtaaat acccaactcc tctcactgta tgaatgtaat caaaacctat tgggacaatt
      181 tttttacgta agcgtgtcat attaacatta agtgtatttt gatcaataaa actatcattt
      241 tcccaaagtt tctctaatag agactctttt gagactactt gtttaggatg catgagcaag
      301 atagatagga ttttattttc tgttggcgat aaaataacct ctttatcttg gctagacaat
      361 aacccttctc ttgtcaacgt aaatccccca aaagttaatt cctgttggat aaattgttga
      421 cttctcctta aaatagcagt tagcttagca tctaatacag ctagagagaa tggttttgaa
      481 ataaagtcat caccccccat atttaatgcc ataaccatat ccatttcatc attactagat
      541 gaaatgaaaa taataggaat tgttaaaaac ttacgcaatt ctgcagtcca gtaaaaacca
      601 ttaaaatagg gtaacgtaat atccattagt atcaaatcgg gttgaaatgc gataatttct
      661 tgtttcacat cacgaaaatt gctgacacta gagacatgat agctagctga taaatgatct
      721 tttaaaagtg acacaatcgt catatcatct tctacaatat aaatttttcc ttgctcttgt
      781 gacatatccc ctcctaaaat agtacgaaat ctctttcttt catttagtat aacaaatttt
      841 ttagtctatc ggtttctatt aatgcaaaag gattggtaaa ataaccaatc ctttttagcg
      901 ttcaataata tgataataag ttcgactagt tatacggtaa atgataaagt aaattataga
      961 aatcgcaagt actgtcaaac cacttactac atatacgatt gttgagttaa gcaccccaaa
     1021 taccaaaagc atctgtttta gcattggaat agcaactcca aagtgaataa ctgctaccac
     1081 taaaggttgg aagaagaaaa tcataatttg agaattaata gtccgtttaa ctaatttttt
     1141 gctcatacct acttcttgta agatacgata agatctctta tcttcatgac cttctgagta
     1201 ttgcttatag taaacaatta gagctatacc tagcaaaaag cttataccta acagaaatcc
     1261 agtaaataat aacccaccaa acatttgata accttctttt agaaaactct gtttagtgct
     1321 aatatttcca acatacttac catctacctc tattatgtcg tttttagaaa ttgaatttat
     1381 ctcttgacta ttcaaatcca aatatgcctt aaaggttttt ggaaaagtat tgatgttctt
     1441 agtataaggt aaatatgctt ttctaatatt gtcaatttgt ttgttgtttg caaatataat
     1501 caaacccggg ttgtaagtat ttgtagtctc tgggacataa gcctctttta aattttttac
     1561 aacgtcaaat ttatttccca atagatttat ttttttcaaa cggctatcac ctttttgaac
     1621 aaagtaagcc acttggttat cttttaattt tggtaattgg tgacctaaac gacggaaatc
     1681 attctgagta attaggtaca taaaacccgt ttttgtgata tcaacatgtt tgacgttttt
     1741 agatgtgata ttaagttcac ttgattgact aactggcatc gaaatcattg tctgattata
     1801 agtgatagct tccttagatg acttacctag tttcttcaaa atcttttctt caaatatatt
     1861 tttcgcgtca ccttttgaat tatctattga taaacttact gattttggaa atagtccggt
     1921 aacaacattt tgtgtatttg aatagagtga gactgttgta gcaatagtaa ctagagccat
     1981 aacagctaat aaagtgatac ttgctaaccc tactgcattt tgcttcattc gaaaaatcat
     2041 ttgcgaagtt gatacaaaat gctcagattt ataataataa tgcttgtttt gacgcaacct
     2101 ttttaagtac catgtcataa aactaatata aaaaagataa gtaccagcaa taactaaaag
     2161 tactgcaaag aagaaacgat agataacagc taatgcaggt gctttacctg aagtaagagc
     2221 cattgtataa gcgatggcga tagctactaa cgctaaaatt gcaagtataa gattaccttt
     2281 tggttctttt tctccctgtt gtttctttct aaaaagactt aatggggatg ataaatgaac
     2341 atgtcgaata actggaactt ctaaagtcag aaaaatacct gtaaatataa cgatacaaat
     2401 aataaatggc cataaactta aactaagatt tagtgcatga tagttaataa tgttgacaaa
     2461 aattaaataa ataaatttag caaaaaaagc actaaacaga cttcctatag aaataagaaa
     2521 tatataaata ataaacagct ctagactagc tacacgcgca acttgacgtt tattcatccc
     2581 caaaatgtta tacagtccaa attcactact acgctgtttc atcaagatat tgtagctata
     2641 atgttccatg actaccgcaa agattgacaa aacaatcact ccaagaaaaa gaaccgttgc
     2701 cgctgtcccc atatctcttc caacagcact cattaagatt agtagcgtag aacaagtcaa
     2761 tgaataaagt aataaactgg ctaagaggaa tggtatgtac tggtctatag aatgttttaa
     2821 attatgccag gctattttga gataaaacat attattcacc ccctaatatg gcagacattg
     2881 ttaaagaaat atctttattg aattcgctat tattcttatt accacgataa agttggtgaa
     2941 aaatacgtcc atctttgata aataaaacgc gtttggcatg actagcagca tttgctgaat
     3001 gggttaccat caaaatagtt tgtccatcca agttaatagt ttcaaataaa ttcagcaagt
     3061 cttctgaatt acggtaatct aacgctgctg ttggttcatc tgctaataaa atttgagggt
     3121 tggttatcaa actacgagca atcgctacac gttgcttttg cccaccagag agctcgaaag
     3181 gtctcttatc taataagtca tcaattctca gatgagaaga taattctgac aaacgatgat
     3241 ccatttcttt gtaacgttta cgatcaagaa ctaaaggaag atagatatta tctcttacag
     3301 agagagtatc caaaaggttg aagtcctgaa agacgaaacc aagatttttc aaacgaaaac
     3361 tcgctaattt tgcctcttta attttcgtaa tatcttcccc atttaaaatc acttgtccat
     3421 ttgtcggttt ttctaaagta gctaaaatat ttagcagagt tgtttttccc gaaccagact
     3481 ctcccataat agcaatgaat tccccttgtt caactttaaa atcgacgtct tgtagagccc
     3541 ttgtctcttc ttttgaaaaa cgtgtacgaa aaactttttc taagtgattg atttctaata
     3601 acatataatt ctcctttatt tattataccc taattttgat tgattctact ctttaaccaa
     3661 gactgtgact cttgtaaagg aaggctggtt acttgatctg gtaagatagg ggtgttctcg
     3721 tatttatatc cttgtcgatc actaactatt cctgtagtta aagctaatct agcaccgtcg
     3781 taaagcatga aagtttcatt aacagtcgta tatcctgctg tagcttgacc aaagctttta
     3841 acattaggta atcctttaaa tgataaaaaa gtcatttctg ccgaactagc agttttatga
     3901 ttagttataa tagcgattgg aacatgctta gtattgattg ttttcctaga tatttttaat
     3961 gcttctagcg gtatattttt catcgtgatt gttttcttat ttccatactt atcagtataa
     4021 tgaaataggg tatcatttgg taaaatagag gctaaccccc caatcatagg gatcatattg
     4081 ccgccagtat tattcgataa gtctaaaatg agtcccttat aacttttttc catcaatata
     4141 ttttgtaatt taccactata atggctaata gatcgtttat ctaacttact tatagaagga
     4201 atagtaatta agagaatgtc ttcgtttaat cttcgatatt ctggatatcg actatctagg
     4261 gtatctcttc gtacagtctc tttggataat atttgactat gtttaccgcc tccataccgt
     4321 actgcttcct gtacaatgtt gcgactctct tgataagatt ttgacgtttt caactttttt
     4381 tctgatcgtt gtcttataat ttgccattga cgaccttgcg aatacaaacc acgctgttca
     4441 acacgatcta aaataacacg tccataacgt tcgctactag gaggtaatag ataaatattt
     4501 aatgctgaac cataataatg aactaccact cctaaaattc ctaaaactat aagcattgga
     4561 actacaaaaa gtaaaactat ttttcgccgc atctcttgct ccccctttct ctttaagtct
     4621 attatatggc aaaatggttt actataacct tacatcttaa gacctatttc ttacaaattt
     4681 gaaagtttta aaagctttat aaactataat tttgatataa tatcttacat aacaaatgga
     4741 aagtacggtg aaaacatatg gccaagacaa gtaaaaaatt gacaaaatct ttagggccta
     4801 ttggtaaatt aatttcaata atccctgaca caactgaact tattggaaaa gcaattgata
     4861 attctcgtcc aattattgaa aaagaacttg accgtcgtca tgaaaaaaac agacttatac
     4921 tcagcttgat aatgtagtac accttgatat caatgatgcg caagtttact tagagcaacg
     4981 ccattttaat gtatcaagga ttgttgctga accaaattca aagtgggcaa ccctacatcc
     5041 taatcaagtt gtgaatatgt cgcctaaagc tggtagggtc agaatcggtt cattagtgaa
     5101 gttgtactat attacagagg atatccttga agaaagtaag acactacttt tatctaaatc
     5161 cgaaaaaagt aaagcacgac aacaacggtt aacagaagta ttgagtaccc ctagtcaatt
     5221 actcaaaaaa aatcgaaata aataagcatg atcgaattga ccatgcttat ttatttttac
     5281 caaacaggtt gatctaaacc ttgagatgtt ttttcgataa cttttttaac cgcatctgta
     5341 tggtaagctt tcacaacttc tttaatggct ttagcttttg atttgtcttg ccaacctttt
     5401 tgagcaacta aaaggttgta ccattgtttt gcatttttac tttttggttc aataaaaata
     5461 gctgatttag gatttattcc tgcttctgtc acaaaatcat tattaataac tgctgcatct
     5521 gtggaatcca aagaacgtgc ggtctgtgct gcatctactt cttttaaatc taaagattta
     5581 ggattggaaa caacatctga cattgttgct aatgtatcac ctgatacttt caattttagc
     5641 aagcctgctg attgtaacaa gtagagagag cgactttcgt taactgcatc gtttgggata
     5701 gtaatagttg ctttatttgg aatttcagaa actgtttggt atttaccttt accgttctta
     5761 gtaccagagt ataatctaaa ggaagtaaag tatgtctcag caacggaaac aagattggtc
     5821 ttatttgctt tattccaatt gtttaagtaa ttataatgct ggaaagcatt aatatcaatc
     5881 tcatcacttt ctaaagcttt attaggttgt gtataatctg tgaattccgt aaattttagc
     5941 ttaacgcctt ttttcttaac taattcttcg attttatccc aacgagcttt ttcagtttta
     6001 ctctttacca ttgttccaac tctaacgact ttggcgtctt catttttaga ctgaccacag
     6061 gctcctaaaa taagtgctga aattactact gttgtcgtta accctaatag ctttttgatt
     6121 ttcataattt atttctcctt tgatatggtt gtatgattaa tcaactgata ctagtgtaac
     6181 aaaaagctaa tggattaaaa aatacttatt atcaatcaag agatatagct tttaactata
     6241 tcatttcttt tataatttta ctttcgaggg ctccgtaaag cggtccaata ataggcgact
     6301 tcctcataac cttggtgctc atacattttt ctgttttctt cttctgaaat taagataact
     6361 ttcttaaacc ccctacttgc atc
//
LOCUS       DATROY010000105         6235 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.106, whole genome shotgun sequence.
ACCESSION   DATROY010000105 DATROY010000000
VERSION     DATROY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..362
                     /locus_tag="VBD11_001600"
     CDS             <1..362
                     /locus_tag="VBD11_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO6954539.1"
                     /translation="KVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKASALT
                     AELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEIDGEI
                     VELDQISLKCQKRYLYM"
     gene            372..569
                     /locus_tag="VBD11_001601"
     CDS             372..569
                     /locus_tag="VBD11_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6954540.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            772..888
                     /locus_tag="VBD11_001602"
     CDS             772..888
                     /locus_tag="VBD11_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954541.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            1132..2247
                     /gene="ychF"
                     /locus_tag="VBD11_001603"
     CDS             1132..2247
                     /gene="ychF"
                     /locus_tag="VBD11_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6954542.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2331..2906
                     /locus_tag="VBD11_001604"
     CDS             2331..2906
                     /locus_tag="VBD11_001604"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6954543.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            2903..>6235
                     /gene="mfd"
                     /locus_tag="VBD11_001605"
     CDS             2903..>6235
                     /gene="mfd"
                     /locus_tag="VBD11_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6954544.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFE"
BASE COUNT         2161 a          853 c         1225 g         1996 t
ORIGIN      
        1 ctaaagtaca agtattagtg gaaggagaga caggacagcg gataaaacta aatgaccttt
       61 attttttcgc attagcaaat aacacttatt ttggaggtgg cattactatt tggcctaaag
      121 caagtgcttt aactgcagag ttagatatgg tttacgctaa agggcatact tttctaaaac
      181 gattaagtat tttattatct ttggttttta aaaggcatac tacctctaaa agtataaaac
      241 accagacttt caaagctatg accgtatatt ttcctaaaaa tagtttgatt gaaatagatg
      301 gcgaaatagt tgagttagat cagattagtt taaaatgtca aaaaaggtat ttatatatgt
      361 aaggagaaaa gatgtatcaa gttggatcac ttattgaaat gaaaaaacct catgcttgtg
      421 taattaaaga aactggtaaa aaggctaatc aatggaaggt actgagagta ggagctgata
      481 ttaaaattca gtgtactaac tgtcagcatg tgattatgat gagtcgttat gattttgagc
      541 gaaaattaaa aaaagtactg caaccatgaa agtcctttta aacaaataat tatagctata
      601 tttgcaacca atactttcct aaataattgc tagtatgccg ttggaaaatt agctattcta
      661 atgttatcga aagaagaaag gtggccattg acaatgacga aagttgcaga acagttgttt
      721 atttttcggt aagtcatatc aaaatgggaa aatggggata ccacaccaga tttggataat
      781 ttaatcaggt tagctgagat ttttgacgtg agcttagatg aacatgtttt agctaaaaca
      841 aatgaagtta aagttgaacg tatttatgaa aaaaatcttg atctgtaaaa atataataaa
      901 atttattggt ttatttttcg aaatattatt cgatctctac taattatctt agctatatta
      961 actttcttat aagctctagg tcttcccttt gtttcggagt ggataattta aatgaaggtg
     1021 aaagagtggc ataatgataa gcaaatctga aattttcttc ttatcatacc gctttttctt
     1081 ctattttttg atataatggt cttgattgaa atttgaatgg agactcacta aatggcttta
     1141 acagcaggta ttgtaggctt accaaatgtt ggtaaatcaa ctttatttaa cgcaattaca
     1201 aaagcaggag cagaagctgc taactatcct tttgcgacta ttgatcccaa tgttggtatg
     1261 gtagaggtac cagatgagcg tctgcaaaaa ttgacagaat tgattacgcc taaaaaaaca
     1321 gttccgacaa cctttgaatt tactgatatt gcaggtattg ttaaaggtgc ttcgaagggg
     1381 gaaggtctag gaaataaatt tttagctaat attcgtgaag tggatgctat tgttcatgtg
     1441 gtacgtgctt ttgatgatga aaatgttatg cgtgaacaag gtcgtgagga tgccttcgtt
     1501 gatcctatag cagatattga tactattaat cttgaattaa tcttagctga tttagagtca
     1561 atcaataaac gttatgcgcg tgttgaaaaa atggcacgaa ctcaaaaaga taaagaatca
     1621 gtagcagagt tcaatgtttt acaaaagatt aaacctgttt tggaagatgg gaaatcagct
     1681 cgtaccattg agtttacaga agaggaagca aaagttgtta aaggtctctt tttattaaca
     1741 actaaacctg ttttatatgt tgctaatgtt gatgaggaca aagtagctga tcctgatgat
     1801 atcgactatg ttaatcaaat tcgtgcattt gcagaaacag agagtgcaga agtagttgtg
     1861 atttctgctc gagctgaaga agaaatatct gaacttgatg atgaagataa gttagaattt
     1921 ttagaagcta ttggattaac agaatctggt gttgataaat taacacgagc tgcttatcat
     1981 cttttaggcc ttggaactta ctttactgct ggtgaaaaag aggttcgcgc ttggacattt
     2041 aagcgtggta taaaagctcc acaagctgcg ggtattatcc attcagattt tgaaagaggt
     2101 tttattcgtg cagtaacgat gtcatatgat gaccttattc aatatggatc tgaaaaagca
     2161 gtaaaagaag ctggccggtt acgtgaagaa ggaaaagagt atattgttca agatggcgat
     2221 atcatggaat tccgtttcaa tgtctagtaa taaataatca atttgttaac ttttaggtta
     2281 aggatattgt tattatcctt aaccattttt gcgttttaat ggagaagata atggtaaaaa
     2341 tgattgttgg attaggaaat ccaggaagta aatataatga tacaaaacat aatattggat
     2401 ttatggctgt tgacagaatt gtaaaaaaac ttgacgtcaa ctttacagaa gataaaaatt
     2461 ttaaagcaga gataggtagt gattttatca acggtgaaaa aatttatttt atcaaaccaa
     2521 ctacttttat gaataacagt ggtattgcgg taaaagccct gcttacttac tacaacattt
     2581 ctattaagga tatgattatc atatatgatg atttagacat ggaagtcggt aaaatacgtt
     2641 ttcgtcaaaa aggatctgct gggggacata atggtatcaa gtctattatt gctcatttag
     2701 gtactcaaga atttgatcgt attaaagtag gtataggacg tccgaatgga cgtatgacag
     2761 ttattaatca tgttctaggg aagtttgata aaaatgatga aattatgata tcaaatactc
     2821 ttgataaggt tgacaatgct gtcaattatt atttacagac gaatgatttt caaaaaacaa
     2881 tgcagaaata caacggttta aaatgaatat tatagaatta tttagccaaa ataaagtggt
     2941 gcggacatgg cactctggat tagtaactaa tagtcgccaa ttagttatgg ggttttcagg
     3001 agcaagtaaa gctattgcta tagcatcagc ctacgagaag ttatctaaaa aaattatggt
     3061 agtcactgcc actcaaaacg attctgataa attatcaagt gatatatcaa gtttaattgg
     3121 agaagataac gtttatcaat tttttgctga tgacgttcct gcagcagagt ttattttctc
     3181 ttcattagac aaatcaattt cacgactcag tgccttgagg tttctaaaag atccagaaaa
     3241 aaacggtgtt ttgattacta gtatcagtgg tttaaggtta ctattaccaa atccggaagt
     3301 tttctcgaaa agtcaatata agtttgaaat cggtcaagaa tgttatttag ataaattgag
     3361 taagaactta gtcaatttag gttatcagaa agtttctcaa gtatttagtc ctggtgaatt
     3421 tagccaaaga ggagatattt tagatatttt tgagatgaca caagaatatc cttatcgctt
     3481 ggaattcttc ggagatgaaa tagatggcat caggcagttt gatattgaca ctcaaaagtc
     3541 tcttaaacaa ttagagtcag tacagatttc gccagcagat gatattattt tacaagatgc
     3601 agatttcgag cgtgcaaaga aaaaacttga gggctatcta gtaacagcta gtgaggaaca
     3661 acgcgcctat ttgagcgagg ttttatctac aaccgagaat cattttaagc actcaaatat
     3721 tcgtcgtttt ctaagtatct tttatgaaaa agaatgggga atattagact atattcctga
     3781 gggaactcca ctttttgttg atgattttca aaaaattgta gatcgaaatg ctaagttaga
     3841 tttagaaata gctagccttc tgacggaaga tttacaacaa ggcaagtctc attctagttt
     3901 gaactatttt tcagatcctt ataagcaatt aagacaatac caaccagcaa ccttcttttc
     3961 aaatttccac aaaggattag gaaatctgaa atttgataaa cttcatcatt ttactcaata
     4021 tggaatgcaa gaatttttta atcaatttcc attgttggtc gatgaaatca atcgttataa
     4081 gaaatctgga gcgacagtac tgttgcaagt agattctcaa aaaggtttaa atctccttca
     4141 agaaaactta aaagaatatg ggttagattt aatcatttca gataaaaacg atatcgttcc
     4201 aaaggagtca caacttattg ttgggcatct ttcaaatggt ttttattttg cagatgagaa
     4261 aattgttctg attactgaac gtgagattta tcataggcgt gttaaaagga aaattagacg
     4321 cagtaatatt agtaatgcag aacgtctaaa agattataat gaactttcag ttggcgatta
     4381 tgttgttcat aatgttcatg gcgtaggaaa atttttaggt attgaaacaa ttgagataca
     4441 aggtattcat cgtgattatc tgactattca atatcaaaat gctgatcgga tctctatccc
     4501 cgtagaacaa attgaacttc taacaaaata tgtctctgca gatggcaaag agcctaaaat
     4561 taatacgtta aatgatggtc gcttcaaaaa agcaaaacag agagttgcaa agcaagtaga
     4621 agatattgct gatgatttgt tgaaacttta tgcagagcgt agtcaattac aaggatttgc
     4681 attttctcct gacgataata tgcaaaatga ctttgataat gattttgcat atgtagagac
     4741 agaggatcaa ttgcggtcga taaaagaaat taaacaggat atggaaggta atcgtccgat
     4801 ggatcgatta ttggttggag acgttggttt tggtaagaca gaggttgcta tgcgtgcggc
     4861 tttcaaagct gtcaatgacc ataaacaagt tgctgtttta gtcccaacaa cagttctagc
     4921 tcagcagcat tttgaaaatt ttaaagaacg ttttagtaat tatcctgtta cagttgatgt
     4981 acttagtcgt tttcgtagca aaaaagaaca aacagataca ctaaaaagat tatctaaggg
     5041 ccaagttgat attattattg gaacacatcg gcttctctct caagatgttg tattttcgga
     5101 tttagggtta atagttattg atgaggaaca gcgatttggt gtaaagcata aagaaaagtt
     5161 aaaagaatta aaaacaaaag ttgatgtctt aactttgaca gcgaccccaa tacctagaac
     5221 attacatatg tcaatgcttg gcattagaga cttatcagtc attgaaacac ctccaactaa
     5281 tcgttatcct gtgcagactt acgttttgga gacaaatcct ggtttagtta gagaagctat
     5341 tattcgtgag atagacagag gaggacaggt tttttatgtt tacaataaag ttgacactat
     5401 tgaacagaaa gtttcagaac ttcaggagtt agtaccagaa gctagtattg gttttgttca
     5461 tggtcaaatg tctgaaattc aattagaaaa tacattaata gactttatca atggagatta
     5521 cgatgtttta gttgcaacga caataattga gacaggagtc gatatttcaa atgttaatac
     5581 cctctttgtt gaaaatgcag atcatatggg cttgtcaacc ctttatcaat taagaggccg
     5641 tgtgggtaga agtaatcgca ttgcttatgc ttaccttatg tacagacctg ataaagtttt
     5701 aacagaaatt tctgagaaaa gactagatgc aatcaaaggg tttacagaat tgggatctgg
     5761 ttttaaaata gcgatgcgtg atttatctat acgtggtgca ggaaatattt taggagcttc
     5821 acaaagtggt tttattgatt cggttggatt cgagatgtat tcacagcttc tagagcaagc
     5881 gattgccacc aaacaaggaa aatcattaat tcgtcaaaaa ggtaatgttg aattagcctt
     5941 acaaattgac gcctatttac cagcagaata tatatcagat gagcgccaaa aaatagaaat
     6001 atataagcgt attcgtgaat tagaaactag ggctgattat gaagccttac aagatgagtt
     6061 aatagatcgt tttggagaat atcctgatca agttgcatac cttttagaaa taggactttt
     6121 aaaagcttat ctggatcttg cctttactga attagtagaa agaaaaggta atgaaataag
     6181 catcatattt gaaaaagcat ctttgaaata ttttttaact caagattatt ttgaa
//
LOCUS       DATROY010000106         6170 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.107, whole genome shotgun sequence.
ACCESSION   DATROY010000106 DATROY010000000
VERSION     DATROY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..106)
                     /locus_tag="VBD11_001606"
     CDS             complement(<1..106)
                     /locus_tag="VBD11_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6954545.1"
                     /translation="MKIGMRKSSLKKSFKARTTGKLKRQIKKKVIPGYG"
     gene            complement(136..435)
                     /locus_tag="VBD11_001607"
     CDS             complement(136..435)
                     /locus_tag="VBD11_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954546.1"
                     /translation="MAIFGGEKLSREEKQKQKIQKYLSQRGIDNLNEKSNVQVRRVIN
                     DLAGNGFFKAGMALSFAKAEEQAKVTYLSALVEQNWILISQNQEILDELKKINNN"
     gene            complement(518..1267)
                     /locus_tag="VBD11_001608"
     CDS             complement(518..1267)
                     /locus_tag="VBD11_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954547.1"
                     /translation="MANLSDNIKYFRKQNKLTQKELARKLKIAPTAISAWEVGRNKPL
                     MDNIEQMASIFGIPKSKLLGDEIYKIQETSSPELIPSTLQKINSTSSQLEHKRQLNVL
                     DYAETQLEQQNIVEDSKNTVVELFSYNYYDHAASAGTGQYLNDVQVETIELPVDYDAD
                     FVIPVYGDSMEPEYHSGDYVFVKLSVELSDGDIGVFEYYGDAYIKQLLIHAEGAFLHS
                     LNDKYNDILIDRDSDFRIIGKVVGNFMPKEH"
     gene            complement(1785..1982)
                     /locus_tag="VBD11_001609"
     CDS             complement(1785..1982)
                     /locus_tag="VBD11_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954548.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSMEAIKKHNLPF
                     IEAEKESAKMSRDALTKVFGR"
     gene            2041..2199
                     /locus_tag="VBD11_001610"
     CDS             2041..2199
                     /locus_tag="VBD11_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954549.1"
                     /translation="MNWKKLMLGDLEHTFTSCNGKEKTSIEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            complement(2298..2939)
                     /locus_tag="VBD11_001611"
     CDS             complement(2298..2939)
                     /locus_tag="VBD11_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954550.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            complement(3156..3491)
                     /locus_tag="VBD11_001612"
     CDS             complement(3156..3491)
                     /locus_tag="VBD11_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954551.1"
                     /translation="MDKNVKQDLLSLMYMLTEDADKQIIISTAAGTYVGNFIPKEKNE
                     KYHTVYAISNKLHQISDTEQTSSDSDVIVLVDVTLISSSHQEFKMPFVYLFTDQIIGV
                     SLGKYSIGQ"
     gene            3567..3782
                     /locus_tag="VBD11_001613"
     CDS             3567..3782
                     /locus_tag="VBD11_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954552.1"
                     /translation="MTKMTLEMARAKVSMTQEEIARKIGVDRNTYASYENYKTPMRID
                     KAINFCKVVNVSIDDIIFLKQNYTSSV"
     gene            3799..4548
                     /locus_tag="VBD11_001614"
     CDS             3799..4548
                     /locus_tag="VBD11_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HEO6954553.1"
                     /translation="MNQLINVTLNENQEPVVSGRDLHKVLEIKTQYTKWLERMSEYGF
                     VENEDFMAISQKRLTAQGNQTEYTDHILKLDMAKEIAMLQRNEKSKQVRKYFIQVEKD
                     FNSPEKIMARALLMADKKITNLTMENNQLQLDLKEAQKQARYLDLIIESKGALRVTQI
                     AADYGMSANKFNKTLLEFGVQHKVNGQWILYKRHMGKGYTDSHTFDYQDKNGHTRANV
                     TTTWTQKGRLFLYELLKDNDILPLIEQEGIA"
     gene            complement(4680..4901)
                     /locus_tag="VBD11_001615"
     CDS             complement(4680..4901)
                     /locus_tag="VBD11_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954554.1"
                     /translation="MSKLSLKPNFEEKKLKTWEDLESQLKFAFDERLTTHITNINPSH
                     FEMSREEITSELKASGYEVIEQGEYLIIR"
     gene            5045..5230
                     /locus_tag="VBD11_001616"
     CDS             5045..5230
                     /locus_tag="VBD11_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954555.1"
                     /translation="MWEKINKIMLERNLNMNKLAKLTGINKSHFSDLKSGRIKHLSWP
                     NMVKLSTGLGISLDEFK"
     gene            5292..5432
                     /locus_tag="VBD11_001617"
     CDS             5292..5432
                     /locus_tag="VBD11_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954556.1"
                     /translation="MKEKKEQWKPQVNCFRKDGQKFKPEDCTIPISFKGFYQILAELK
                     GV"
     gene            5434..5658
                     /locus_tag="VBD11_001618"
     CDS             5434..5658
                     /locus_tag="VBD11_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954557.1"
                     /translation="MENPITGVVALLFIALIAYIGNRNSNQKTITKTVDTILDDYQVV
                     KKVERKQRTDFIELPTPGSCGKIWGKDRPF"
     gene            5734..>6170
                     /locus_tag="VBD11_001619"
     CDS             5734..>6170
                     /locus_tag="VBD11_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO6954558.1"
                     /translation="MHIEEVKNNQFYQFPQWLLKEPYNVLSDKAKLIYMLLFDRRTLS
                     VENKWFDDDGKVYMYFTNEQFMELLKCSEKTIIKSKKELSNFGLLNEVRQGINKPNRL
                     YINGTVKVTGQDLNNLQHGTVKVTGQDLNNLQHGTVKVTGQDL"
BASE COUNT         2147 a          995 c         1099 g         1929 t
ORIGIN      
        1 tcccatatcc aggaataact ttctttttaa tttgtctttt tagttttcct gtggttcgag
       61 ctttaaatga tttttttaaa ctagattttc tcattcctat tttcatatca atcccctaaa
      121 tcaagcgtgg aatttttagt tattattgat ttttttgagt tcatccaaaa tttcttggtt
      181 ttgactaatc agtatccaat tttgttccac taatgctgat aagtatgtaa cttttgcctg
      241 ttcctcagct tttgcaaaac tcaaggccat gcctgcttta aaaaaaccgt taccagccaa
      301 atcattaatc acacgtctta cttgcacgtt agatttttcg ttcaagttgt caattccacg
      361 ttgagataaa tatttttgta ttttttgttt ttgtttttct tctctagata atttttctcc
      421 gccaaatatt gccatagtaa ttctcccatt attaaattaa attttaaaaa tcatttacag
      481 tataaaaact aatcattcct ataatgcgta atgcgactca atgttctttt ggcataaagt
      541 tgccgacaac ttttccgata atacggaaat cactatctct atctatgagt atatcgttat
      601 acttgtcgtt cagactatgc agaaatgccc cctctgcgtg tataagtagc tgtttgatat
      661 aagcgtcacc gtaatactca aacactccta tatcaccgtc tgaaagttcc acagataatt
      721 ttacaaatac gtagtcgcca gagtgatatt ctggttccat agaatcgcca taaaccggta
      781 tgacaaaatc agcgtcataa tcgactggta attcaattgt ttctacttgt acatcattca
      841 gatactgccc tgtaccagct gaagcagcgt ggtcgtagta gttgtaggag aataattcta
      901 ctactgtatt cttactatct tctactatgt tttgttgttc taattgtgtt tcggcataat
      961 caagcacgtt tagttgtcgc ttgtgttcta attgagaaga agtagagttt attttttgta
     1021 gggtagatgg gataagttct ggtgatgagg tttcttggat tttatatatt tcgtcaccta
     1081 aaagcttcga ttttggtatt ccgaaaatag aagccatttg ttctatatta tccattagag
     1141 gtttgtttct acccacctcc caagctgaaa tggctgttgg agctattttt aactttctag
     1201 ctagctcttt ttgggttagc ttattttgtt ttctgaaata ttttatatta tccgataagt
     1261 tagccatctt ttgctccttt actattatat gtctaacaat attgtacact tcaagtggaa
     1321 taaatgcaat atttttttag caaaatgcaa aaaaaacact tgcaagtaca cttcaagtgt
     1381 agtataatat aatcaagctt aaggaattaa gcaaaacgaa aggaggtaca gctgatgaag
     1441 tctaggctaa acaaaaagcc taaacacaaa gaagtcgagt tggaaattca cattctttgg
     1501 tttaagctaa aaataaaata ttcgattacg tggtaatcgg atagggggtg aaattccccc
     1561 accccattta gggggtaagt ttagtttagc acattggctg tatctccgca agaatgaaag
     1621 gagagcagat ggaagaaaat aacatttata attttcatgc agtacttgga attatgctac
     1681 gtaatattca agaagattat atggaaataa aaaaccctct agcaaggtgt gagcttgcta
     1741 aaggatactt tgagattggt agatatcttt acgacgaagg tgtattacct tccgaatacc
     1801 ttggtgagag catctctaga catcttagct gattcctttt cggcttcaat aaaaggtaaa
     1861 ttatgtttct taatagcttc catagagtct tcgtaaagtt tgattttatc ttcgatagac
     1921 aaagtagggc tagaagaagc gacaatagcg agagctaaat cttttgaatt agaaattttc
     1981 attagcttat cctcctttcc actaggataa gttgattata acatttttag gaggtacaaa
     2041 atgaattgga aaaaactaat gctaggcgat ttagaacaca cgtttactag ttgtaatggc
     2101 aaggaaaaaa caagtattga atttgaaggc ggcgtattgc cagcgctatt ggtgctaggt
     2161 ggtatcactt ggctgattgc ttggtttatt acaaaataaa aactcccaag agggagtgga
     2221 aaggagggaa ggcatggaaa aaattaaata cggtgtacta ggttcttcca agatatttca
     2281 cacaagaaaa tctgctctca ctcatgcaaa tcttttagga tatcctcgta gtgcggtttt
     2341 tttgataatt ccacagggaa tgacgcagaa aatgattgat aaggtgaatt aacatatcct
     2401 gctttattag ctttaaatag ttttttcttt ttagctactt taaaagcaac aaagagtcta
     2461 tctgtgattt cagacgtttg tatgattaaa tccatccttg tcatactgtg ggcttttaat
     2521 actccacaat ctgcctctgg aacctcgata aggattgtat tgccatcagg ctgtactgcg
     2581 gctatagcct ctctacctgt taaatcgtta attatattat tttgcttttg gtaataatgc
     2641 tgatattttc tgtttttatc aaaaacaatc aagtcgaagt agcttacatc aacattagaa
     2701 ggattgatga ttttaatgtt agctcttaat gtaccatttg gattatatat gctttcaccg
     2761 ttgtctaaaa taacgctcaa aatccaatct gaaacaggag cagcaattaa ctcgacttgt
     2821 aagttatttc ttcggtagtt tgaataagat agaaacagag ctattaaagc tatccaattt
     2881 tttattagat attcacttgt aaacttaaaa acacacaata aaaaattaaa aaaattcatt
     2941 tcaacctcac atttttattt aaattatacc acagaaagga ggtgggggaa tggacaaaga
     3001 cagagaagag tttttgatta gattaattga tttgcacgag catatcacca gtaagtgttt
     3061 agagctcaat agagagctaa tcaattatct aaaggaacaa aaagaaccta ttgaattaac
     3121 aatagattcc aaaacgttag ccgagaaact agagattact gaccaatcga atatttccca
     3181 agcgaaacac cgattatttg gtctgtaaat aagtagacaa acggcatttt gaattcttga
     3241 tgtgaagatg aaatcaaggt cacatcaact aagacaatca catcagaatc agatgaagtt
     3301 tgttcagtat ctgaaatttg atgtaatttg ttactgattg cataaactgt gtgatatttt
     3361 tcgttttttt cttttggtat aaaatttccg acataagtac cagcagcagt tgaaattata
     3421 atttgtttgt ctgcatcctc agtcaacata tacatcagac ttagaaggtc ttgtttaaca
     3481 tttttatcca tagcgttatc ctcctttcgt taagatttcg aggatattat agcaattatt
     3541 caaactttta gaaagggggt gggggaatga cgaaaatgac actagagatg gcaagagcca
     3601 aagtgtcaat gacgcaagaa gaaatagcta gaaaaatcgg tgtagacagg aatacatatg
     3661 ctagctacga gaattataaa actccaatgc gtatcgataa agctattaat ttttgtaaag
     3721 ttgttaacgt atcaattgac gatattattt ttttaaaaca aaactacact tcaagtgtat
     3781 aacactagaa aggacaatat gaatcaacta attaacgtaa cactaaacga aaatcaagaa
     3841 ccagtagtaa gcggtcggga cttgcacaaa gtacttgaaa ttaaaacaca atatacaaaa
     3901 tggcttgagc gaatgagcga atatggtttc gttgaaaacg aagacttcat ggctattagt
     3961 caaaaaagac taacagctca gggtaatcaa actgaatata cagaccacat tctaaaacta
     4021 gacatggcaa aagagattgc tatgttacag cgaaatgaaa aatcaaagca agtccgcaaa
     4081 tacttcatcc aggtagaaaa ggacttcaac agtccagaaa aaatcatggc acgggcgttg
     4141 cttatggcag ataagaaaat cactaactta acgatggaaa ataatcaatt gcagttagat
     4201 ttaaaagaag ctcaaaagca agcgcgttat cttgatctaa tcattgagag caagggagct
     4261 ttgcgagtaa cacagattgc tgctgattat ggtatgtctg ccaataaatt caataaaaca
     4321 ctattagaat ttggtgtgca gcacaaagtg aatggacaat ggattctcta taaacgacat
     4381 atgggcaaag gttacacaga cagtcacaca tttgactatc aagacaaaaa cggccataca
     4441 agagccaatg tgacgacgac gtggacgcag aaggggcgct tgttcctata cgagttgtta
     4501 aaagataacg atattttgcc actcattgaa caagaaggta ttgcatagaa aggaaacgca
     4561 tgagaccaaa acaatatccg tatagcggaa ataaaaaaga atctattgcg gtaacagtag
     4621 attctgaaaa attagtcctt ttacaagaac aaatttcgat tcttgaagag agacttaact
     4681 tatctaatga ttagatattc tccttgttcg ataacttcat aaccacttgc tttcaattca
     4741 gaagtaatct cttctctaga catttcaaaa tgagacgggt ttatgttagt tatgtgagtt
     4801 gtaagtcgct catcaaatgc aaattttaat tgagactcta aatcttccca agtttttagt
     4861 tttttctctt caaaatttgg tttcagactt aatttactca taaccttcct cctttccata
     4921 attttttgaa tacaacggtg agaggtcata ttcaaataaa ttatatcaga aaggaacgaa
     4981 agacacaaca tattgttata taaataaaat ttgaacacaa tatgttgatt tttggagaaa
     5041 tattatgtgg gaaaaaatca ataaaataat gcttgagaga aatctcaata tgaataaatt
     5101 agcaaaacta acagggatta ataaaagcca ctttagcgat ttaaaaagtg gcagaatcaa
     5161 acatctatct tggccaaaca tggtcaagtt atctacaggg ctaggtatca gcttagacga
     5221 atttaaataa atcaaaaaag ccactgtggg gacagtgacc tcaagaaaat taataatgag
     5281 gtaattataa catgaaagag aaaaaagagc aatggaaacc acaggttaac tgtttccgaa
     5341 aagatggaca gaagtttaaa cccgaagatt gtacaatacc tatttcgttc aaagggtttt
     5401 atcaaatttt agcagagtta aaaggagtat aacatggaaa atccaattac aggcgtagta
     5461 gccttattat ttattgcttt aattgcgtac atcggaaacc gcaatagtaa tcaaaaaaca
     5521 attacaaaaa cagttgacac aatcttagat gattatcaag ttgtcaaaaa agtagagcgc
     5581 aaacaacgca cggattttat agagctacct actccaggat cgtgtggaaa aatctggggc
     5641 aaggatagac ctttttaagg agtattgaat ggcagacaat aaaaaatact actatcttaa
     5701 attaaaagaa attttttttg aaagcgatga atgatgcata ttgaagaagt aaaaaacaac
     5761 caattttatc agtttccgca gtggttgtta aaagagccat ataacgttct gagcgataaa
     5821 gcaaaattaa tttatatgtt gttgtttgat cgcagaacac tatctgtgga aaataagtgg
     5881 tttgatgatg acggtaaggt ttatatgtac tttacaaacg agcaatttat ggaactactt
     5941 aaatgctcag aaaaaacaat cataaaatct aaaaaagaac tgtctaattt cggcttatta
     6001 aacgaggtca gacagggtat aaacaaaccc aaccgcttat atatcaatgg aactgtaaaa
     6061 gttacaggtc aagacctgaa taatttacag cacggaactg taaaagttac aggtcaagac
     6121 ctgaataatt tacagcacgg aactgtaaaa gttacaggtc aagacctgaa
//
LOCUS       DATROY010000107         6115 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.108, whole genome shotgun sequence.
ACCESSION   DATROY010000107 DATROY010000000
VERSION     DATROY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            151..1434
                     /gene="aroA"
                     /locus_tag="VBD11_001620"
     CDS             151..1434
                     /gene="aroA"
                     /locus_tag="VBD11_001620"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6954559.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1427..1939
                     /locus_tag="VBD11_001621"
     CDS             1427..1939
                     /locus_tag="VBD11_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6954560.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            1996..3369
                     /locus_tag="VBD11_001622"
     CDS             1996..3369
                     /locus_tag="VBD11_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6954561.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            3470..4825
                     /gene="rlmD"
                     /locus_tag="VBD11_001623"
     CDS             3470..4825
                     /gene="rlmD"
                     /locus_tag="VBD11_001623"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6954562.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            4933..5154
                     /locus_tag="VBD11_001624"
     CDS             4933..5154
                     /locus_tag="VBD11_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954563.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            5272..6009
                     /gene="aphA"
                     /locus_tag="VBD11_001625"
     CDS             5272..6009
                     /gene="aphA"
                     /locus_tag="VBD11_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6954564.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
BASE COUNT         2141 a          901 c         1204 g         1869 t
ORIGIN      
        1 aacaatatta taacataatg atgtatgaca tgtgaagtta atttataaaa gatgttacag
       61 ctatatagag gagctatagt ataaataaaa aattcaaaat atagcatata ttatgataca
      121 ataatagcaa aatagatttt gggagttatc atgaaattac tgactaatgc aaatactctc
      181 aagggaacca tccgtgttcc tggcgataaa tcaataagtc acagggctat tatttttggg
      241 agtatatctc agggagttac ccgaattgtt gatgtcttaa gaggcgagga tgttttatca
      301 actatagagg cttttaaaca aatgggggtt ctaattgaag acgatggcga gataatcact
      361 atctatggca aaggatttgc aggtttaaca caaccgaata atctcttaga catgggaaac
      421 tctggtactt caatgcgtct gatagcaggt gtgttagctg gtcaagagtt tgaagtgacg
      481 atggttggag acaatagttt gtcaaagaga cctatggatc gtatagcatt gcctttaagc
      541 aaaatgggag ctagaatttc gggagtaact aaccgtgatt taccaccatt aaaacttcaa
      601 ggtacaaaaa aattaaaacc tattttttac catttacccg tagcttctgc acaagtaaaa
      661 tcagctttaa tttttgcggc tttacaaact aagggagaat ctctcattgt agaaaaagag
      721 caaacaagga atcatactga ggacatgatt cggcaatttg gtggacattt ggatattaaa
      781 gataaagaaa ttcgactcaa tggcggtcag agcttagtag gacaagatat aagagttcct
      841 ggtgatattt cgagtgctgc cttttggata gtagcgggtc ttattattcc taacagtcat
      901 attattttag aaaacgtagg tataaacgag acacgaactg gaatccttga tgtcgtcagt
      961 aaaatgggtg gaaaaattaa gttatcttcg gttgataatc aagtaaaatc agcaaccctt
     1021 acagttgatt actctcatct acaagctact catatttctg gggctatgat tccacgtctg
     1081 attgatgaat tacctattat tgctctttta gcaacacaag ctcaaggcac cacagtcata
     1141 gcagatgctc aggaattaaa ggtgaaagag acagatcgga ttcaggtcgt tgtcgaatcg
     1201 ctaaaacaga tgggtgctga tattactgct acagcagatg gtatgattat acgtggtaat
     1261 acccctcttc acgctgcttc acttgattgt catggagatc atagaatagg aatgatgata
     1321 gctattgctg ctctcttggt caaagaaggc gaagtagatt tatctggtga agaagctatt
     1381 aatacaagct atcctaactt tttagaacat ttggagggat tagttaatgc ctaaggtatt
     1441 attgggattc atgggagttg gcaagactag tgttgccaac tgcttagaaa atgaagtaat
     1501 tgatatggat agtctgattg aaaaacatat tggtatgtca atttcccgat tttttacaga
     1561 agaaggagaa gcctcctttc gtgctttaga atctcaattt ttaaacgaac ttttaaagaa
     1621 aaaaaatgaa ggacttgtga tagctagtgg aggaggtatt gtactccttg aagaaaatcg
     1681 tcgtctttta actttaaatc gtcataataa tattctttta acaggaagtt ttgaagtctt
     1741 atatcatcgt ataaaaaaag atgagaagaa tagaagacca ttatttttaa accattcaaa
     1801 agaagaattt tatgatattt atcaaaaacg catgttactc tatagtggtt tgtctgatat
     1861 gattattgat accgattatc ttacaccaca aaaaatagct actgtaattg gagagtcact
     1921 ggttaattta tctaactaag ttatcacagt ttggtagtat gaactattat tattgaaagt
     1981 aaatcacctg aaaatatgtc aagaaataat tacggtcagc taaatcatca tgaagagcta
     2041 cgatataact atcttttaaa aaacatccat tatcttaatg aacgtgaaaa gatggaattt
     2101 cagtaccttc attataaaaa aacagcagta cgaccacaga gaaggacaga aagtccacca
     2161 actaattctt attatgagga accttattct gattcttatt atcaagatga cgatttttac
     2221 tcagagcctc agctaacctc acaaggttta ccaatttatc aagaagagag agctccgaag
     2281 aagaaaaaac aaagggccag aaaagaaaaa cagagggtga aggttatggc gcctttccct
     2341 ccaaaggcta taaccccccc tagaaagaaa aaaaaattta agggtttcct aaagttcata
     2401 ggtattatat tactaattgt tttatcaggt atggttttca tgtttgttaa aggaatgcgt
     2461 gatgtgaata atgggaagtc acattatagc cctgcaatta ttgaagattt taagggaaaa
     2521 gatgctgttg atgggactaa tatcttaatt ttaggtagtg ataaacgtgt cagcgagaga
     2581 tctactgatg cgagaacaga taccataatg gttgctaatg ttggtaacaa ggataataag
     2641 gttaagatgg tgagttttat gcgagatctc ttagtcaata ttcctaatta tagtacggaa
     2701 ggttactatg atatgaaatt aaatgcgtct tttaatttgg gagaacaaga taatcataaa
     2761 ggagcagagt atgttcgaca aacgttgaaa aatcattttg atattgatat aaaatattat
     2821 gttatggttg actttgagac atttgcagat gctattgata cactgtttcc taatggtgtc
     2881 aagattaatg ctaaatttgg acttgtcggt ggacaatctg ctgattcagt taaagttcct
     2941 gatgatttaa ggatgaaaaa tggcgttgta cctagccaaa aaattaaggt tgggattcag
     3001 tacatggatg gaaggaccct tctaaattat gctcgtttca gaaaagacga tgatggggac
     3061 tttggtcgaa cacaacgtca acaacaagtt atgagagcta ttgtctctca aataaaagac
     3121 ccacgtcgtc tctttacagg ttctgctgct atcggtaaag cctatgcttt aacttcaagt
     3181 aatttatctt atagctttgt cctaacagat ggcattccaa ttttatcaga tgctaaaaat
     3241 ggtattaagc aaatgacaat tccaagagaa ggagattggg tagatgatta tgaccagtat
     3301 ggtggtcaag ggttaacaat tgattttgct aagtacaaaa agatcctaaa gaaaatggga
     3361 ctgcgttaac atttttaatt atgatataat agagtgattt attgtcactc tatttttatc
     3421 tttagtgtat aaactaaaga cttcaaatga tagatataaa aggaaaatta tgttacataa
     3481 aaatgatatt attgaaactg aaattagcga cattagccat gagggaatgg gtattgccaa
     3541 agtagatggc tttgttttct ttgttgaaaa tgctcttcca ggtgagatta ttaagatgcg
     3601 cgtcttgaaa ttacgtaagc gtataggata tggaaaagtt gaggaatatt taacgacttc
     3661 tcctcaccga aatgaaggat tagactatac ctatttacgt acagggattg ctgatttggg
     3721 acatcttact tatgaacaac agctgttatt taaacaaaaa caagtagctg ataaccttta
     3781 taaaattgct catatatctg atgttttagt agaacctact cttggtatga caatcccttt
     3841 ggcatatcga aataaagctc aggtaccagt tcgtcgtgtt gatggacagc tagagacagg
     3901 ttttttcaga aaaaattcac atactctggt atccattgaa gattatttaa ttcaggaaaa
     3961 agagattgat gctctgatta attttacacg agatttatta agaaaatttg atgttaaacc
     4021 atatgacgaa gagcagcaat ctggtttaat tagaaatctc gttgttcgtc gaggacatta
     4081 tacagggcaa ttaatgttgg ttttggtgac aacacgtcct aaaatattcc gcattgacca
     4141 aatgattgaa aagttggtgt ctgcctttcc ttctgttgtt tcaattatgc aaaatattaa
     4201 tgatcgtaat agcaacgtta tttttgggaa agaatttcgt actctatatg gttctgatac
     4261 tattgaggac caaatgttag gaaatactta cgctatatca gcccaatctt tttaccaagt
     4321 taatactgaa atggctgaaa aactgtacca aaaagcaatc gatttttcag atttaaactc
     4381 agaggatatc gttattgatg cctattcagg tataggaaca attggcttat ctgttgctaa
     4441 acaagtaaaa catgtttatg gtgttgaagt tgttgaaaaa gcagtaagtg atgccaaaga
     4501 aaatgctact cgaaatggga taactaacag tacctatgtt gctgattcgg ctgaaaatgc
     4561 aatggcaaag tggcttaaag aaggtataaa accaactgtt ataatggttg atccaccacg
     4621 aaaaggatta acggagagtt ttgtatattc tgctgcccaa acaaaagcag ataaaatcac
     4681 ttatatttct tgtaattcag cgacaatggc gcgtgatatc aaactatttg aagaattagg
     4741 gtatcattta gtaaaaattc aaccagtaga cttattccca atgacacatc atgtggagtg
     4801 tgtggctctg ctcgtaaaag cctagaaact ttttaacgaa aggaatatta aaaagcctta
     4861 gataatcgac tgacctatta ccaaaaaatc gaaggggctc tacttgccat tgcacaaaca
     4921 ccaggaaata gtttgccaac tatgcagctg ttagatgagt tagttcttcc aaaagacatt
     4981 accatagcag atgtcagtca aagtatccaa gaatttgaaa tagaaagaga tgcccttcaa
     5041 gaattttttg acgatatgct gaagacttat acaacctacc aagaaatgaa agattatatt
     5101 aaatctcgtg aagcgaacac tcctaagcaa gagacagaag aaaaatcact ttgaagactg
     5161 tagacttaac ataagtaaaa aataaccata tatgatatcg ctttcattct tatgtttttt
     5221 gtgataaact aattaaagaa ataattttta tactttatta tggaggttct tatgaagaaa
     5281 gtgttagtga gtagtctttt ggttttaggg attacgataa cgttacaaac agtagttgag
     5341 gctaaggggc caaaagtagc ttatacacaa gagggaatga ctgctctttc ggacacaaat
     5401 aaagataaag tcactactat ttctattgac gagattcaaa aaagcttaga aggtaagaag
     5461 ccgattactg ttagttttga tattgatgat acactgcttt tcagtagtca atattttcaa
     5521 tatggtaaag aatatgtaac tcctggatcg tttgattttc ttcataaaca aaaattctgg
     5581 gatcttgttg caaaacgagg agatcaagat tccattccca aagaatatgc taaaaaatta
     5641 attgctatgc atcaaaaacg aggagataaa attgttttta taacaggtag gacaagaggg
     5701 tcaatgtata aggagggcga ggttgataaa acagctaaag ccttagctaa agattttaaa
     5761 ttagacaaac caattgctgt aaattataca ggcgataaac ctaaaaagcc atacaaatat
     5821 gataaatcat attatattaa gaaatatggt tcagacattc attatggaga tagtgatgac
     5881 gatattcatg cagctaggga ggccggtgct agaccaatta gaattttaag agcacctaat
     5941 tctacaaatc tacctttacc agaagctgga ggctacggtg aagaggttct cgaaaattca
     6001 gcttactagt atctttatta gttaacatag tattatctat caatttttag atttgtaata
     6061 agaagtgcta aaaaagtagc aatcaatgat atgctaataa gggaagttgg agtag
//
LOCUS       DATROY010000108         6070 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.109, whole genome shotgun sequence.
ACCESSION   DATROY010000108 DATROY010000000
VERSION     DATROY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            154..441
                     /locus_tag="VBD11_001626"
     CDS             154..441
                     /locus_tag="VBD11_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6954565.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            453..944
                     /locus_tag="VBD11_001627"
     CDS             453..944
                     /locus_tag="VBD11_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6954566.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            989..1228
                     /locus_tag="VBD11_001628"
     CDS             989..1228
                     /locus_tag="VBD11_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6954567.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1398..2342
                     /locus_tag="VBD11_001629"
     CDS             1398..2342
                     /locus_tag="VBD11_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6954568.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2367..3038
                     /locus_tag="VBD11_001630"
     CDS             2367..3038
                     /locus_tag="VBD11_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6954569.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3170..5998
                     /gene="uvrA"
                     /locus_tag="VBD11_001631"
     CDS             3170..5998
                     /gene="uvrA"
                     /locus_tag="VBD11_001631"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6954570.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
BASE COUNT         1940 a         1015 c         1211 g         1904 t
ORIGIN      
        1 aaataaaaaa tgtctttaaa tgatatcagg gaaataaaaa cataaaaacc ttgcattatt
       61 aacttatttc tactataata atatcttgtg agcataacct cacttactca tgaaaaattc
      121 atgagtcatt agaccaaaag gaggaacata tcaatggcta aatacgaaat tctttatatt
      181 attcgtccaa acattgaaga agaagctaaa aacgctttgg tagcacgttt tgattctatc
      241 ttatctgaca acggtgcaac tgttgttgaa tcaaaagatt gggaaaaacg tcgtcttgca
      301 tacgaaatcc aagatttcac tgaaggactt taccatatcg ttaacgttga agctgaagat
      361 gcagtagctc ttaatgagtt tgatcgtctt tcaaaaatca acggtgacat tcttcgtcac
      421 atgatcgtta aagtagacta agaaggtagt ttatgattaa taatgtagta cttgtaggtc
      481 gcatgacccg tgatgcagaa cttcgttata caccaagtaa tcaagcggta gccacttttt
      541 cacttgcagt taatcgtaat tttaaaaatc aatctggcga acgtgaggct gattttatta
      601 actgtgttat ttggcgccaa caagctgaaa acttggctaa ctgggcaaaa aaaggtgctt
      661 tggttggaat tacaggtcgt atccaaacgc gtaattatga aaaccaacaa ggtcaacgtg
      721 tctatgtaac agaagttgtt gcggaaagtt tccaattatt ggaaagtcgt gctacacgag
      781 aaggtggttc acctaactct tataataacg gtggctataa caatgctccg tcaaataata
      841 gttattcagc ttcttctcaa caaacaccta attttagtcg tgatgagagt ccgtttggta
      901 actcaaatcc aatggatatt tcagatgatg atctaccatt ctaatataat agatggacag
      961 aatttaaaac tataaaggag aaataaacat ggctcaacaa cgtcgtggcg gattcaaacg
     1021 ccgtaaaaaa gttgattata tcgcagctaa caagattgaa tatgttgatt acaaagatac
     1081 agagcttctt agccgtttcg tttcagaacg tggaaaaatt ttaccacgtc gtgtaacagg
     1141 aacttcagct aaaaaccaac gtaaagtaac aacagcaatc aaacgtgctc gtgttatggc
     1201 tcttatgcct tacgtaaatg aagattaata taaaatagcc ctagcggcta tttttttatt
     1261 ctttttacca ttattattgg tatttacgag tcaaataata gaaaaattta ggataccacc
     1321 gtattaaccg tgcaaaacaa atggttttcc tgtataatac taaggtatgg attttttata
     1381 acaaaggagg aatcgccatg aagcaaatgt ttctttcaac ggcaattgaa tttaaagaaa
     1441 ttgaaacatt cgagccaggt gcttggataa atctggttaa tccctcccag gaagaatccg
     1501 aacaagtagc tgatcaattt ggtattgata ttgatgattt acgtgctccc ctcgatgtgg
     1561 aagaaacttc ccgtatctcg gttgaagatg attatacttt agttattgtt gacgttccta
     1621 cctatgaaga acgcaataac aaaagttatt atatgactat tcctatgggc attatcgtta
     1681 ctgacaacgc tgttattact acttgcctag aacatttgac gctgtttgat cacttttata
     1741 gacgacgtgt caaaaatttt tataccttta tgaagacacg ttttgttttc caattgcttt
     1801 atcgcaatgc cgaattatac ttacaggctt tgcgtactat cgataggcag agcgataaaa
     1861 ttgaagcaca actagagagt gcaactcgaa atgagcaatt gatcgatatg atggaattgg
     1921 aaaaatctat tgtctatcta aaggcctctt taaaatttaa tgagcgaatc gtcaaaaaat
     1981 tgaccagtag taccagtagt ttaaaaaaat acattgaaga tgaagatctg cttgaagata
     2041 cattgatcga aactcagcag gctattgaaa tggcaaatat ctatgaaaat gtcttaaatg
     2101 ccatgacaga aacgactgct tcaatcatcg gtaataatca gaataccatt atgaaaacct
     2161 tagccctagt aactatgaca ttggatattc caacggttat tttttcggcg tatgggatga
     2221 actttaaaaa taattggatg cctttgaatg gtctcgcaca tggctttatc tatgttgttc
     2281 ttttggcctt tttgatgagt tcatttgttg tattttattt tattcgtaaa aaatggtttt
     2341 aatagtatat taatcaagga gttcacatga ctttacaaga cttaactaaa aagaatcaag
     2401 agtttgttca tattgcaact aatcaacttt tagctgatgg aaaatcagat gcagaaatca
     2461 aagctatttt agaagaacat ctcccagaga ttatagataa tcagaaaaaa ggaattaccg
     2521 cacgctcact cttaggagca ccaactactt gggcagcctc attcactgag cgacctgagg
     2581 ataaagctag agtatctgta caaaaaaata ccaatccttg gttgatgtgg ttagatactt
     2641 ccttactatt tcttggctta gtaactgctt tgaatggttt aatgttgcta tttggtcaat
     2701 ctaatgtcaa tacagggctt atttcaattt taactcttgg ttttggtggt ggtgcggcca
     2761 tgtacgttac ttattactat atttatcgcc atatgggtaa accaaaatct gaaagaccag
     2821 gctggcttaa atctttcgca gtactggcac tagttatgtt agtttggttt gccttatttg
     2881 cagtagtacc tcttctacct gcaactatta atcctaaact tcctgaggtt gttttgttta
     2941 ttattgcttt agcatcgttt ggtttgcgct tttatttaca acgtaaatat aatattcaaa
     3001 gctcaatggc accagtggca ccacaacaaa gaagataata acaaagagta tgaacgtatt
     3061 agttcatctc ttttttctat gaaatttaaa actaaaaaaa tactgacttt attaaaattt
     3121 gatataataa gtcaacacta ttcaaaaagg agtgctgagg cacaacatta tgcaagataa
     3181 attaatgata agaggagcaa gagctcacaa tttaaaaaat ataagtgtcg acattccacg
     3241 agacaagtta gttgtcgtta cgggactatc aggatcaggt aagtctagtt tggcctttga
     3301 cactatctat gcagaaggac aacgtcgtta cgttgaaagt ctttctgctt acgcgcgcca
     3361 atttttggga aatatggaaa agcctgatgt agattctatt gatggcctta gtccagctat
     3421 ttctattgat cagaaaacaa caagtaagaa tcctcgctct actgttggga ctgtaacaga
     3481 aattaacgat tatttacgtt tgctttatgc tcgtgttgga accccatatt gtatcaatgg
     3541 tcacggagcc atcacagcat cttctgtaga gcaaattgtg gataaggtac tagcgcttcc
     3601 agagcgaaca aagatgcaaa ttttagcccc aattatacgt cgaaaaaaag gacaacacaa
     3661 atcaacattt gagaaaatac aaaaagatgg ttacgttcgt gttcgtattg atggtgatat
     3721 tcacgatgtt actgaggtgc ctgaattatc aaaaagtaag atgcacaata ttgatattgt
     3781 agtagatcga ttaattaata aagagggcat tcgttcccgc ttatttgatt ccgttgaagc
     3841 ggctctacgt ttgtcggatg gttatgtcgt tattgataca atggatggca atgagttatt
     3901 gttttcagag cattattctt gtcctgaatg tggttttact gttcctgaat tagagccacg
     3961 ccttttttct tttaatgctc cctttggttc ttgtccgacc tgtgatggct taggaattaa
     4021 gttagaagta gatatagatt tggttattcc agatagaagt aagaccctta gagaaggggc
     4081 cttagttcct tggaatccaa tttcatcaaa ttattatcca acgatgctag agcaagctat
     4141 gactcaattt ggtgttgatg tggatacgcc atttgaaaaa ttaagcaagg cggagcagga
     4201 cctagcccta tatggttcag gcgaacgaga gttccatttc cattatatta acgattttgg
     4261 aggcgagaga aatattgatt tgccttttga aggggttgtc aataatatta atcgtcgtta
     4321 ccatgaaact aatagcgatt acacacgcaa tgttatgcga gaatatatga acgagctaaa
     4381 atgtaacact tgtcatggct atcgtctcaa tgatcaagca ctttgcgtaa gagttggggg
     4441 agaagaaggt cttaatattg ggcaagtctc agatttatcg atagcagacc atttagaact
     4501 tttagaaaca ttgcgtttat cttcaaatga acaattaatt gctcgaccaa ttatcaaaga
     4561 aatccatgac cgtctaagtt tcttaaataa tgttggactt aattatttaa atctgtcacg
     4621 ttctgcagga acactttccg ggggcgagag tcaacgtatt cgtttggcta ctcaaattgg
     4681 gtctaattta tcaggtgtcc tttatgtact tgatgagccg tccattggac ttcatcagcg
     4741 agacaatgac cgtttgattg atagtcttaa gaaaatgcgt gatttgggta atacgcttat
     4801 tgttgttgaa catgatgaag atacaatgat ggcagctgat tggttaatcg atgtgggacc
     4861 tggcgctggt gcgtttggtg gtgagattgt ggcctctggt acaccgaagc aagtagctaa
     4921 aaatactaaa tctattacag gacaatattt gtcaggtaag aaagtgattc ctgtgccatc
     4981 tgaacgaaga gtaggtaatg gtcgcttttt agaaatcaaa ggtgcggcag aaaacaacct
     5041 acaaaattta gatgtcaagt tccctctcgg taaatttatt gccgtaactg gtgtctcagg
     5101 ttctggaaaa tcaaccttaa ttaatagtat tttgaaaaaa gctgttgcgc agaagcttaa
     5161 ccgtaactca gataaaccag gtaaatatgt ttctttagaa ggcattgaat atgttgatcg
     5221 attgattgat attgatcaaa gtccaattgg tcggactcca cgttctaatc ccgcaaccta
     5281 tacaggtgtt tttgatgata ttcgtgatct ttttgctcaa actaatgagg caaagattcg
     5341 aggttataaa aaaggacgct tctcatttaa cgtcaaaggt ggacgttgtg agtcttgttc
     5401 tggcgatggt atcatcaaaa ttgagatgca ttttttacca gatgtctacg ttccttgtga
     5461 agtatgccac ggcacgcgct ataatagtga gactctggaa gtacattaca aagaaaaaaa
     5521 tattgcacaa attcttgata tgacagtgaa tgatgcagtt acattttttg cagctattcc
     5581 taaaattgct cgtaagttac agactattaa ggatgttggt ttgggatatg tcactttggg
     5641 gcaaccagct acaaccttat caggaggaga agctcagcga atgaagctgg caagtgaatt
     5701 acataagcgt tcgacaggta agagtcttta catattagac gaaccaacaa cggggcttca
     5761 tgcagatgat attgctcgtt tacttaaggt tttggatcgt tttgtagatg atggtaatac
     5821 agtgcttgtt attgagcata accttgatgt tattaaaact gctgaccaca ttattgatct
     5881 tggccctgaa ggaggtattg gtggcggaca aattgtagct atcggaacac cagaagaagt
     5941 tgctgaaaat cctaaatcct atacaggata ttatttaaaa gagaagttag caagatagtt
     6001 tattgggagc tgagtaatat tactcagttc ctttttgtaa gctaacattg cgtagaagtc
     6061 attaaatgga
//
LOCUS       DATROY010000109         6018 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.110, whole genome shotgun sequence.
ACCESSION   DATROY010000109 DATROY010000000
VERSION     DATROY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            60..5384
                     /locus_tag="VBD11_001632"
                     /pseudo
     CDS             60..5384
                     /locus_tag="VBD11_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            5536..>6018
                     /locus_tag="VBD11_001633"
     CDS             5536..>6018
                     /locus_tag="VBD11_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6954571.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
BASE COUNT         2186 a          944 c         1159 g         1729 t
ORIGIN      
        1 gtgcttaaat taagcttgct taacaaataa aataatctaa cttaaaaatg agtggaatta
       61 tgaaaagaat ttctttaaaa acaaccggtg tactgattgc gtttttagct gtaaatgcgg
      121 tagcgacctc tgtagtggta gatgcagaca cagtagtccc tcatactagt gcgaatacta
      181 gtcagctttt agaaaaaaac acaactatca agccttccga agttaaacag tcacgtactg
      241 aaagtaatgc agtaacccct ccaacaatag cctcgagtaa ctcacaaaag cttgagaata
      301 aatcatctgt agattcaaat atactaaaga gtgaacaacc tgcacataaa gttgctggtg
      361 ttaaacaagt cgttcaagaa caaacgaaaa atcaaaaaga tgaaacctct ttacaggcta
      421 aaaagcaagt acaatcaagt gaaaagttat taaatacttc atcacaacct tcaagtgagg
      481 aaaacaaaaa cactaataag cctaataagg ctaatgaagt ggatatgaaa aatgtcgtga
      541 ataatgcttc gacgaagcca actcccccat ctgattctaa agtgtataag cgcgttattg
      601 taacatttga tcaagtaaat aaggaaaaag tagatgaagc ccttaaagca attccgggca
      661 ctcgtattaa gttaacttat agtagccttt ttaatggcta ttcattagca gtagatgaaa
      721 aagaaattga aaaaattggt aagattaaag agataaaaca tattgaagca gttcaagtat
      781 taaaacctca aatgtttact gctaaccaat ggacacatgt cttagctgca cgtttaaaag
      841 ctaaacaaaa tagtaaagtt gacggtagag ggctacttat tgcgactatt gatagtggtg
      901 tagatatcca tcatcctgcc atgcgattgg atgagaatga tccagatgtg atggcaaagc
      961 gtcgtattaa agatattaag gctccttata caaataaagt tccttttgca tacaattatc
     1021 aacgaggcga taatttagaa ttgaaagacg acgttcagcg ccctcatggt atgcatttag
     1081 caggtatctt agcgggaaat gcaaaggatg gttttaaagg agttgcccct aatgcacagt
     1141 tattagtgta caaaatttca acaaatagag aagatccatc tgcagagtgg accggctctg
     1201 atgcagtctt ccatgcaatg gaagatgccg ctaaaagagg ggcagacgtt attagtttaa
     1261 gctttggata tttgggaaca ggacgtccag gtgaactttg gtatgagaca attaagaagg
     1321 tgactgacaa gggtgttatc gtagtagcag caatgggaaa ctatggtaat tcggcttcat
     1381 ctactaccta cgataatgtc gttgacaact ctttgggaac aaaagatact gctgctatgg
     1441 tgtctgttgc agctgttgat aaggttattg gagttggttc agttcaaaat agtgctcttc
     1501 ttttagatgt tttgaaagtt ggaaatctta aattaggata tgcagaactg ggacataaaa
     1561 gtcaaacaat attaaaaatg ctcaaaaata aaacatttga tctagtctat gttggtaaag
     1621 gccagttgaa aaaagatatc tcagaagagg accttaaaag ttatactgga aaagttatcg
     1681 tcgttgagcg tggaggagag ggcgttaaag ataagctaaa acgttttatc ggaattgcta
     1741 aaggggttat tatgataaat gaacctgctt attatagttc tgggaatttt gatactcacc
     1801 ccatgtttga ttatcactat gatataccag aaggtaaatg ggctattagt ctatcaaaaa
     1861 aagatggtga tcagttgatt gactacatca agaaaaacaa aactgctaaa ttagaatttt
     1921 tacctaaaga aacctttgtt cgccgtccaa atcctacaat ttcaggcttt tctagttggg
     1981 gagtgacacc agatttgcat ttgaagccag atgtagttgc tccaggagaa gatatttatt
     2041 ctacttttaa cgacggtacc tatgggtaca tgtctggaac ttcaatggct acccctcaag
     2101 tagctgccat tagtgcatta gtaaaaagta aagtagctga aataatggca ttaaaacttc
     2161 caatgttagc tggtttgaca aacgttgatt tgacaaagat tgtcttgatg aatacaagtc
     2221 aacctttgaa agatactcta aataaagctt tagaaatttc accacgacgt caaggtgcag
     2281 gtatggttaa cttagaaaaa gctttagcta acgatgttct tgtttttggt gataaaacag
     2341 gatcattctc tataaaagag attaagaacc ataaaagatt taccttgaaa ttggtcaata
     2401 caggacaaaa acgtcgtaac tttagtattg aaccaggagc gattttaaca actaaaattg
     2461 ttgataaagt gactaatcag gggtatgaaa atgtcgtttt aaaggcagtt cacgctaaag
     2521 taatagaggg agcaacttta caaatgggta aagttatctc actagcaccg caatcagcca
     2581 tagagcttga tatagatctt gagataaaaa aacctataca agattttgct gaagggtatt
     2641 tgtatttcaa aactttagat aaagatggac aagatatctc agctccttac atgggcttct
     2701 caggaacatg ggatttagaa ccaatttttg acgcacctat gtgggatagt cgttctaaaa
     2761 ataaattaac gaccttgatg caagcaactt tggttggaca cggaactaac aaattttatg
     2821 aaatcggaca agaaataggt agtaaaaagg tcaatccatc cctatttact atgtctaatg
     2881 tgcgtgtagc tcccgataaa gttgtgcgtg tagcacctcg attttcttta ttacgctcag
     2941 catcagattt tgacctctct attgtatcag aagcaaaaga cgattcacca atacttcgtc
     3001 gtataacagt gaaacatttc gtggagaaat attacaaatc aagaaatgag gaagaagact
     3061 catattatga tgctttgaga acgacagaat catcgctagt ttgggatgga aagttttatg
     3121 atcctaagac tggtgatttt aaagctgcaa agccaggctt atattacttt aagttacgtg
     3181 cacgttcaaa tcctacagat ccttatcaag tccaatacct tccaatttta atagataatg
     3241 aaagaccaac tttttctctt aaaaaagtta ataacggctt tgatatttta acccatgata
     3301 atcatcgtgt taaagaagta attgttcgta ataaagagac acaaaagcct atcacagtta
     3361 aaaaaattgc cgaaaatcac tatcatattg atgagttaac gttaagagaa tctgatctgg
     3421 atataaaagt tgcagactat gctgggaatt caaacaagga aattgcttta tcagaatcag
     3481 aagaagtatc agagaaatct tactctagtg aggaagataa tacattacaa ccacagtcac
     3541 tgtctcatcg caatatgaaa gctcaagcaa ccagttcaga tgatgacgat atctgggatg
     3601 ataacgatga tgaccttaac gaagaaaaag attttgattt tgaagaaaat atttctgata
     3661 tcgaggatgg tttattatta actcgagaaa cggtatattc aattcgaact gtaaataaaa
     3721 ttcgaaatgt tgaaaatgaa agcttggagc atagtgtttc tctcttatta aaagatggct
     3781 atcgtgcaga aataagagtt gttaaccttc atgagaacgc taaagatcca aaacattccg
     3841 acccatatcg tacagtatcc aaccctattt atatgtatgg tgatgactct gacacattta
     3901 aaaccacatg ggtcaaattt atgtctggga ataacatcat gaatttgaag gtttataata
     3961 agaatgataa attggtattt agtcgtggct atcatattac cgttgatgta caacctccaa
     4021 aattagaact ggacaagact aacatagaag aagctgctga caaagataac tttatcactg
     4081 gtcaaatcta tgctcaaaat ggagaaattg aaatcaaagg gcacgtttct gataatgtaa
     4141 aaggttgggg tctttctatt aatggtgata gcgtcgatgt taaatattca agtggtgaat
     4201 ttatcagtaa tagatgagca tttactcata agataaaagc agcagcagga gatattgtta
     4261 cgatttcagc ggtagataca tcaggtaata ccgtttctga acattataag gtagttaatg
     4321 atgtggatac accgaagtta actattaatg aagcagattt attatccgga aaagtcaaag
     4381 tggttatagc taaagcagaa gataatcacc ttaaaaattt aatgataact gttaatggca
     4441 agccctacac agaaggacaa agtattgact cttaccaatt aaaaggtttc gaagggcgtt
     4501 acgtcatcaa agtatcagca gttgataaat caggtaaagt cactgaaaaa attataacaa
     4561 tcggcaactt ccctgaatta tcagcaagtt tacgcaaaac ctctttccaa atgcaagatc
     4621 ttacctctat cgataattta ttatccctag cccacaatca gactgcggaa ttgattaagc
     4681 cagtccaact catggacaaa aaagtgagct ttacggcaaa aatatctgac ccccttggtc
     4741 gttctgtggt tagagagttt gaagcattat tatcaacttc aaaccttaaa gtagattcaa
     4801 aactaaaacc acatttgaaa aaacaaactc tccttttaac agatccaaaa ggaattacgg
     4861 accttttcga cttggataag gatagctcta ttttattgac agaaatagtc gataataaag
     4921 tgggaaatca tactataaag ggtttgatat ttaaaaatgg tgcatataac catttcgaaa
     4981 caagtataca aattgtaaaa acaatggaag aaatcaactc tgacagacgt ttgaaaaatg
     5041 atcataaaag ctctgacgac acaaaactaa aaaaagaaac agagtcgtta aaagggcata
     5101 aggttgatcc agcagttcat ggtgaattta aagaaatatc taaacatcaa atggataaac
     5161 cacaagtgat agctgtaggc atgaaaggga gtacaaagac acccccattt gttcatagtc
     5221 aaatggaagc gaagaaaaca actaagattc caatgtctag agcggcaaaa catgaattgc
     5281 caaggacaag tgatgacaat aataatttac tggctaaagg aattgcactc atagtaattg
     5341 tacttggttt actaggcctt aaaaaagctt caaagaaata ttgattttga ttaaattagt
     5401 tttctaattt attaagctaa aattcctttg gataaattat taagggagtt ttaacttaat
     5461 ctattatttt gtagaaaaat tgataaataa atggtattgt aactctatca ctaaatttat
     5521 atactagagg aaataatgtc acgcagtcaa aaagttattt taactaatat gtgtcttatt
     5581 gaagataata aaggacgtat tgtgatgcaa atccgtgatc ctaaacgtta ttcttggtca
     5641 ggagctgctc taccaggcgg ccatattgaa gaaggagaaa gcttgtatga tgctctcgtt
     5701 cgagaagttc gcgaggagac aggtttaaca ataagtaatc ccaaattagt tggtatgaag
     5761 cattggtaca cagatgaagg catccgttat ttggtattcc tttataaaac atatgatttt
     5821 tcaggtgaat tacaatcgtc agatgaaggt gatgttatat ggattgaacg ttcacaactt
     5881 gataagattg aactagcata tgatatgctt aaccttttga gagtatttga cgaagatgac
     5941 atctcagaga tgttttatag taagcgactt gaagatggtt ttataagaaa aattttatta
     6001 aacaaaaaag aactagga
//
LOCUS       DATROY010000110         6011 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.111, whole genome shotgun sequence.
ACCESSION   DATROY010000110 DATROY010000000
VERSION     DATROY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(74..577)
                     /locus_tag="VBD11_001634"
     CDS             complement(74..577)
                     /locus_tag="VBD11_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954572.1"
                     /translation="MKLNKIILSTAALTALFLGYNSVTADTYNNYQPHRSNNMDLTEE
                     YNYNNQIELQERIKNLNIPFKEKLALLRKVKSRQDSYVLDTLRKEVENKNSEYSELEQ
                     EYQRISALINAKFPQGKSSLAEKLHKELIWDLDGVKYDEGISKRTTLKKLEGKLNEYT
                     KKYSYLF"
     gene            913..1332
                     /locus_tag="VBD11_001635"
     CDS             913..1332
                     /locus_tag="VBD11_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6954573.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            1329..1625
                     /locus_tag="VBD11_001636"
     CDS             1329..1625
                     /locus_tag="VBD11_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954574.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(1689..2996)
                     /locus_tag="VBD11_001637"
     CDS             complement(1689..2996)
                     /locus_tag="VBD11_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6954575.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(3005..3565)
                     /locus_tag="VBD11_001638"
     CDS             complement(3005..3565)
                     /locus_tag="VBD11_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954576.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(3519..4028)
                     /gene="tsaE"
                     /locus_tag="VBD11_001639"
     CDS             complement(3519..4028)
                     /gene="tsaE"
                     /locus_tag="VBD11_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6954577.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(4111..5532)
                     /locus_tag="VBD11_001640"
     CDS             complement(4111..5532)
                     /locus_tag="VBD11_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6954578.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(5635..>6011)
                     /locus_tag="VBD11_001641"
     CDS             complement(5635..>6011)
                     /locus_tag="VBD11_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6954579.1"
                     /translation="HFVGDVLYNKTNLILYILYVSSILFGFLSGIESFSVIMRKFRIS
                     NIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKPQVVINELLAVPSRDSFHFILG
                     FTFLQVLCLIFMDYSAGKKLEK"
BASE COUNT         2034 a         1113 c          874 g         1990 t
ORIGIN      
        1 tgtcttttta gtatcaatat aataaatgca aagtaatcag aatattaggc tataaaatta
       61 ccaaagtcaa ttatcagaat aaataactgt attttttagt atattcgttg agtttacctt
      121 ctaatttttt tagagtagtt ctcttagaaa tcccctcatc atatttgaca ccatccaagt
      181 cccaaattag ttctttatgt aatttttctg ctaaactaga tttcccttgc ggaaacttag
      241 cattaataag agccgaaatt ctttggtatt cttgctctag ttctgaatac tcactgtttt
      301 tattttcaac ttcttttctt aatgtgtcta atacataaga atcttgtctt gatttaacct
      361 ttctgagtaa ggcaagtttt tctttaaaag gtatatttag gttttttata cgctcctgaa
      421 gttctatctg gttattatag ttatattcct cagttaaatc catattattt gatctatgtg
      481 gctgatagtt attatatgta tccgcagtaa cgctattata tcctaaaaag agagcagtaa
      541 gagctgcagt agataatatg attttattta atttcaaaat agttttcctt ctatgataaa
      601 tctcaattat catttttatt ttatttatat tatttattat acatttcatt agataaaaat
      661 gtcaatcatt atatttaaat ttattttaga tacttccaaa attctagaaa tgtacttact
      721 caataataaa catcattaaa aattgcaata ccctttaggt catgctaaga taagtatgat
      781 ggtaaatata gatatcacaa tcaatacagg ctacttttat ccacctgtac gacttatgac
      841 ctatcaaacg ataaaaattt accattcctt aatagctacc caataaattt ttcaacatga
      901 aaggaattag ttatggataa ttgtattttt tgtaaaatca tcagtggaga aatctcttca
      961 tcaaaagttt atgaagatga tgaagtatta gcttttctcg acattacaca aactacgact
     1021 gggcacaccc ttcttattcc taaaaaacat gttcgaaatg tgttagagat ggatgaaaaa
     1081 actgcacaga taacctttga acgcttgcct aaagtggcgc gtgctgtcca agctgctaca
     1141 aaagccaaag gaatgaatat tattaacaac aatgaggaaa ttgctggaca aactgttttt
     1201 catgcacacg tccaccttgt tccccgcttt gatgaatcag acggtatcaa aattcactac
     1261 actactcatg aacccgactt tgaggcactt gcaaaattag ccaaagaaat tagaaaagag
     1321 attcaattat gaaaataggt cgtttaattg cactaggttt agttagttta ggagcgcttg
     1381 aactttacaa aaatagaaaa actatcaagg attcctatca aaatacaaaa aatgagacag
     1441 attcagctaa attaaaatta gaacgtatca aaaatgatct tgctattatt agtcaggaaa
     1501 aagaaaaaat cagattaatt tctcaagaac tcaatcataa attccaagtg tttaataaag
     1561 atattcaacc tcgtttagaa gaaattaatc aacgcatggc taaatatcaa gagaaagacg
     1621 aataagccga cggtataaaa atatctactc taaatataaa aagagcaaat aatgctcttt
     1681 tttatatttt atggattagt ttgacttggt actggttgcg ttcctgtgtt tggattaggc
     1741 gttgcagccc cgttataatt attaacactt ccacttgaat cagcctgacc tgtgttagtg
     1801 ttactgctat agttactagc aggagtgcta ctattatagt aactttgatg agttgtttga
     1861 ttagtctcag aactataagt agtattacca ctgtaacttg gtggtgcttc tgaataaggt
     1921 gttgtattat aattattctc ttgtgttgat gaataagtag aagaatcatt actagcagta
     1981 gtaccatagt aatcttcata tagaatcgcg cttgtcttca gagttttact acgcttttta
     2041 tccagctctt tcttaattct attttgaact gcaagtagat gtttcttagt taaaatttga
     2101 taagagccac catctgatag agtagcgtct tcacccttca actgataaga tttaatatgt
     2161 tccaatgaat ctttataagc taacaaatta ggaatcgttt ttgatgatat ctcaatatta
     2221 gtttgcatgt tattacttac tgcggaaaga atttttttgt atgaactaat actatttaac
     2281 gccaatattt ttttaaggac tttttgaatt acttcacgtt gtcttttttg acgcccataa
     2341 tctccctctg gatcatcata gcgcatacga gaataaacaa gtgcttgttc tccatttatt
     2401 ttatgtgtcc ctggttcaac aacagccttg tactctggtt cattggcagc aattgatatt
     2461 ggaaagtcaa atttattagt tactgttata ccaccaacag cattgaccaa atcaactaat
     2521 ccttgcatat taatttgcat aaagtaatca acattaatat ctaataagtc ttgaacagtc
     2581 atcaatgcca tttccgcacc accagaagca taggctgcat ttagctttgc ttctacgcca
     2641 gtctgtccat tatttttggg accactcaat ttaatcaata cgtcacgttc taagcttgtc
     2701 atcgttgttt tattagtttt aggatttata gtgactaaga tcatagaatc gctatttcct
     2761 gaccacttag attttcgatg ctctgaacct gtgtccacac ccattaatag tattgaaaac
     2821 ggctttgttt cttcaatagc atgacttttt gatttagcta gcttaaaatc tttaaaagtc
     2881 ttagacaatt cattagtcga aaaattataa gctgaggcaa catagactcc caatgcaatt
     2941 actgttgtta aaataattgc agaaaacatt aaggttattt ttttccaaat tttcatgtat
     3001 attcctaatc tatcaactta ctcattctgt aaatgtctag aaattctcca ttttctcttt
     3061 taatacctct tgtttgaagc ccatctattt caaaactgaa tttttggtag aggtgaatag
     3121 cacgttcatt acgtccttgg actgatagtt ctaagcggcg tgtaatatcg ttatctgacg
     3181 cccattctat tgcttcttcc atgagaatat gaccaatacc atacccccaa taatccttct
     3241 gaacagcaat aaaaagatcc ccaatgtgtt caatttcaat atcagactga ctaatgattg
     3301 ttatcaagcc gatcacttta ttttttaatt tagcaatcaa acaaatgcaa tttttcgtta
     3361 ttaaggtatt ttcaataaac gtttccattt ctggcaaaga tagttgattg ctcaccgtat
     3421 gcaaaataaa atctgtttcc tcagtaacag tatttaaaaa ttcaagaagc gcagatgctt
     3481 cctctctcga agcttctata aaatcaacag caaattgttt agacatcttg aatcgcctca
     3541 ataatttctc ttgcgcgatg cccataagct tctaactgaa cttgacgtcc ttgattgctc
     3601 ctcgtaataa caatttctaa gtagttattg ataaggttat cactaagcaa ctctccccat
     3661 tcaataactg ttactccttg accaaacaag aagtcatcta agtcaataga atcaggatca
     3721 tctccaatac gataaacatc caaatggtaa agtggtacac gcccttcata ttctctaaca
     3781 atagtgtaag ttgggctctt aatcatttgt tttatgtcta atccctttgc aatacctttt
     3841 gtaagagttg ttttgcctgc acctaaatta ccagtcaata atacgatatc tcctgacttt
     3901 aaaacagtac ccagtttttg ccctagggca ataagctctt cttcattttg agtataaaac
     3961 atagagatat tataccagaa atatctctgt cttccaacag tttgctgatt ctgacagccc
     4021 catagcatat atttcttcac ttttcttaag aaaaaaggaa accaggctac aaatctgatt
     4081 tccttttatc ttatttatca aactataata ttacaaaata gccaagctga taaagtttag
     4141 aataaataat atatctaaaa tccacagtac cacatgaata tcttttgctt gtcctttgat
     4201 gactttagct agtgtatagg tcaagaaacc agctgcaata ccataagtaa tgctataagt
     4261 aaaccccata aaaagagaag taaagaaagc tgggatagct tctgacatat catcccattt
     4321 gatatctttt aaatttgaaa gcatcataat accaacaata accaatattg gtgcagtagc
     4381 ttgactcgga acgatagata ctagtggact aaaaaaacta gatatagcaa acaaaccagc
     4441 aactactaag gctgtcaatc ctgtacgacc accagctccg attcctgctg ctgactcaac
     4501 ataggttgta acgttagatg ttccacaaat agcaccaaaa gttgtcccaa ttaagtctga
     4561 atagagtgct ttgtctaaac tcttagattc atgattatca ccagtcgttg ctaagatacc
     4621 aactttttct ccggtaccaa tgagcgttcc aatggtatca aaaatatcag tcagtgaaaa
     4681 agcaagaatg gccattaata cacttggtag acgtgaagga ttagaaaata aagaaattaa
     4741 tcctttctct cctaatgcaa ctccaaatac ttgcttcaaa tctctaaaag aggccgagag
     4801 gttagttgct tcccaattaa tagcatctaa cttaaccact cccattaaaa tacctaagag
     4861 ggtcgttgtt aatatcgata gaataatccc tccacggata ccttttacaa taaagaagat
     4921 ggtaatgctt aaaccaatca aagataataa aattgctgga ttatcaaacg acactagccc
     4981 tggcgttgct gaagaatttg ctgtaatagt agccaatcct ttcgcagcac cctttccaac
     5041 aacatcatac gtcccaggat caattgaaaa tttcaaaaaa ccagcatttt taatcccaac
     5101 gtatgctaaa aaagttccga ttcctgctgt aatggctgat tttaaagttg ttgggataga
     5161 ctcaataatc atctttctaa ctttcgttaa agtaataatc agagaaatca aaccacaaat
     5221 aaataccatg gctaaggctt cttgccatgt atatcccaat gcaaagacaa ctgtatatgt
     5281 aaagaaagca tttaatccca tcccaggagc ttgtgcatag ggaaggttag catagaaagc
     5341 cataacagat gtagctacaa cggcaccaat aatcgtcgct aagaaaactc cttgtgctgg
     5401 catcccagtc tgagataaaa tagctggatt tacaaacaaa atgtacgaca ttgcaaagaa
     5461 agtcgttaga ccagcagtga tttcagtccg aattgtcgtc ccgtgttcct ttagcttaaa
     5521 aaacttttcc attttaaaat cggattctcc tttatttttt gttattttac acttttaaaa
     5581 aaattaatcg ttcgtatatt tacgaacgat taatatatat taacaaaaaa taattcactt
     5641 ttcaagcttt ttccctgcag aataatccat aaaaataaga cacaatacct gtaaaaatgt
     5701 aaatcctaaa ataaagtgga atgaatccct actcggaacc gctaaaagtt cgttaattac
     5761 cacttgtggc ttcgttaaga tatcccatga atttagacgg gcataacgac caatatgaat
     5821 accaaaactt gaaacaaatg atacaattcc aataattccc catcttaaaa atatattgct
     5881 aattctaaat ttacgcataa tcacagaaaa actttcgata cctgataaaa aaccaaacaa
     5941 gatactagat acatacaaga tataaagtat taaatttgtc ttattataaa gaacgtctcc
     6001 gacaaaatgc a
//
LOCUS       DATROY010000111         5989 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.112, whole genome shotgun sequence.
ACCESSION   DATROY010000111 DATROY010000000
VERSION     DATROY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            174..1832
                     /locus_tag="VBD11_001642"
     CDS             174..1832
                     /locus_tag="VBD11_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6954580.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            1947..2861
                     /locus_tag="VBD11_001643"
     CDS             1947..2861
                     /locus_tag="VBD11_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954581.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            2871..3902
                     /locus_tag="VBD11_001644"
     CDS             2871..3902
                     /locus_tag="VBD11_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954582.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            3915..4964
                     /locus_tag="VBD11_001645"
     CDS             3915..4964
                     /locus_tag="VBD11_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954583.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            4961..5893
                     /locus_tag="VBD11_001646"
     CDS             4961..5893
                     /locus_tag="VBD11_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6954584.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         1959 a         1000 c         1181 g         1849 t
ORIGIN      
        1 aattattatt ttcagataat tgaatgaaga aaacgtttgt tataaaaatg taaaaaaatg
       61 taaaaaaatg aaagttaaaa aattatctga aaattttgcg tttttgtctt ttcttaagaa
      121 ttttttgtgg tataatgtta gtcactagat aaatgaatta ggagaacaaa gctatggcaa
      181 aacagaaaaa taactggcgc cgtgttggag ttggtgtcct tacacttgct tcagttgcga
      241 ctcttgctgc atgtggaagt aaatcagctt cccaggattc taatggagcg attaattggg
      301 ctattccaac agaaatcaat acactagatt tatctaaagt tacagacact tactcaaatc
      361 tagctattgg taactctagt agtaatttcc ttcgcttaga taaagatgga aagacaagac
      421 cagacttggc tactaaagtt gatgtttcaa aagatggctt aacttataca gctacattac
      481 gtaaaggctt gaagtggtca gatggcagta aacttactgc aaaggatttt gtttattcat
      541 ggcaacgttt agttgatcct aaaacagctt cacaatatgc ttaccttgct gttgaagggc
      601 atgtgcttaa tgccgataaa atcaacgaag gacaagagaa agacttgaat aagctaggtg
      661 ttaaggcaga aggcgatgac aaagttgtta ttactttatc tagtccgtct ccgcaattca
      721 tctactacct tgcattcact aacttcatgc cacaaaaaca agaagttgtt gaaaaatatg
      781 gaaaagatta cgcaactact tcaaaaaata cagtttactc aggaccatat actgttgaag
      841 gttggaatgg ttcgaatggt actttcacgc tgaagaaaaa caaaaattat tgggacgcta
      901 aaaatgtaaa aacaaaagaa gttcgcatcc agactgttaa aaaaccagat accgccgttc
      961 aaatgtataa acgtggtgag ttagatgcag ctaatatctc aaatacttct gctatttatc
     1021 aagctaataa aaataataaa gatgtcacag atgttctaga agcgaccact gcctatatgg
     1081 aatataatac tactggttct gtgaaagggc ttgataatgt taagattcgt cgcgccttaa
     1141 acttagcaac taaccgtaaa ggagttgttc aagcagccgt tgatacaggc tcaaaaccgg
     1201 caattgcttt tgcacctact ggtttagcca aaacaccaga tggaactgat ttggcaaaat
     1261 atgttgcccc aggttatgaa tataataaaa ctgaagcagc aaaactcttt aaagaaggtt
     1321 tggctgaatc aggcttgact aagctaaaat taacaattac agcagatgct gatgctcctg
     1381 ctgccaaaaa ctctgttgac tatatcaagt ctacttggga agctgctctt ccaggactta
     1441 ctgttgaaga aaaatttgta acctttaaac aacgcttaga agacagtaga aaacaaaact
     1501 ttgacattgt agtttctctt tggggtgggg attatccaga aggatcaact ttctacggcc
     1561 tctttaagtc ggattcacaa aataacgatg gaaaatttgc taacaaggac tatgatgctg
     1621 cttataacaa ggcaatttct gaagatgcga tgaaaccagc agaatcagcg aaggactata
     1681 aagaagcaga gaaaatttta tttgagcaag gtgcttataa cccactctac ttccgtagtg
     1741 gtaaaggttt acaaaatcca aaattaaaag gtgttattcg taatactaca ggtttgtcaa
     1801 tagactttac acatgcttat aaaaataatt aaattacttt acactaaacc tttgcaagaa
     1861 ttggtaattg ccagttcttg cttttggtta agtaaagaaa gtgaaagagt ttgctatttt
     1921 tagcaattct aagaaaagga aatgatatga ttaaatatat tttaaagcgt gttgctattt
     1981 tgttagtaac gctatgggtt gtcataaccc tatcgttctt tctgatgcag atccttccag
     2041 gaacacccta caataatcca aaactcactg aagaaatgat cacattgctt aataaacagt
     2101 atggtttaga caagcctgta tggcaacaat atttgactta cctttggaat gtacttcatg
     2161 gtgattttgg aacgagttat caatcagtga atcagccagt atcacgtatg attagcttga
     2221 gattaggagt ttcagtacat ctaggtgttc aggctctcgt atttggtgtt ttaggcggta
     2281 ttttggtagg agctatttcg gcacgtcata agaatgataa agtcgatggt atcttaagtg
     2341 ttattgcaac gctagggatt tctatgcctt catttatcat tggtatctta ttattagact
     2401 attttggatt taagtggaac ttgttgcctt tatcaggttg ggggactttc tctcaaacaa
     2461 ttttaccttc tttagctcta ggactcccta cattagcatc tgtttcacgc ttcttccgta
     2521 gtgaaatgat tgaaacttta aattctgatt atgtgcaact agctcgttca aaaggtatga
     2581 caattcgtca agtaactcga aaacatgctt accgtaattc tatgattcca attttaactc
     2641 taattggccc tttggcggcg ggtctgttaa ctggttctgc actaattgag cagattttct
     2701 cgattccagg aattgggcag cagtttgtta cttcgattcc aacaaaagac tatcctgtta
     2761 ttatggggac aaccattgtt tatgcagtaa tgttgatggt tgctatcttg ataacagacg
     2821 ttgttattag tatcgtagac ccacgtgttc gcttgcagta aggagatgtt atggcagata
     2881 aaaacagaac atttaaactt gtaggtgcag gatcttctag cacacaagaa aaaattgaaa
     2941 agcctgctct ttcgtttatg caagatgcgt ggcgtcgctt gaaaaaaaac aaattagcag
     3001 tagtttcact ctatttatta gctcttttac ttactttttc gttagcctca aatttatttg
     3061 taactcagaa ggatgctaat gggtttgatt cgaaaaaagt aacgacatat cgcaacttac
     3121 cacctaaatt gagttcaaac cttccttttt ggaatggtag cattaagtac gctggaaata
     3181 cagaatctac agacgcttat aagtctcaaa atgtccctga aaaagttaag tatgctttag
     3241 gtacagattc tctagggaga agtgttgcta agcgcatcat cgtaggtatt cgaatttcct
     3301 tacttgtagc tattgcagct acattcattg atttaattat cggtgttaca tacggtcttg
     3361 tttcaggttt tgctggagga cgccttgata cattaatgca acgtgtcgta gaggtaattt
     3421 catcaattcc aaacttagtt attgttacaa tgttaggttt ggtccttggt aacggtatta
     3481 cggctattat tatctctatt gcttttaccg gatggacttc tatgtctcgt caggtaagga
     3541 atttaaccct atcatatcga gaaagagagt ttgtgcttgc tgcacgttca cttggggaaa
     3601 gtccaattaa aatagccttc aaacatattt tacctaacat ttctggtatt atcattgtac
     3661 aaattatgat gacaatccca agtgctatta tgtatgaagc ggtactttca gcaatcaatt
     3721 taggtgttaa accaccgaca gcttcattag gatcattgat ttcagatgca caagaaaatt
     3781 tacaatatta tccatatcaa gttatcttgc cagctttagc attggtaatg atttcattgg
     3841 catttatttt gcttggtgat gggttgcgag atgcttttga tccaaaatct agcgatgaat
     3901 aaggaggtat aatcatggaa aaagaaacta ttttaagtgt taataatctt catgttgact
     3961 tccacacata tgctggagaa gtaaaagcaa ttcgtgatgt caactttgaa ttaaaaaaag
     4021 gtgagactct tgcaatcgtt ggtgaatctg gttcaggaaa atctgtaact accagaactt
     4081 taattggttt aaatgctaaa aattcagaga tatcagggaa tgttcaattt aaggggcgta
     4141 accttgttga actatcagaa gaagagtgga ctaaggtacg cgggaatgag atttctatga
     4201 ttttccaaga ccctatgact agtttggatc caacgatgaa aattggtatg caaatagcgg
     4261 agccaatgat gattcatcaa aaaatttcaa aaaaggatgc tttaaaatta gctcttgagc
     4321 taatgaaaga tgtaggtatc ccaaatgctg aagagcatat taacgattac ccacatcagt
     4381 ggtctggagg aatgcgtcaa cgtgcggtta tagcgattgc tttagctgct gatcctgaaa
     4441 ttcttattgc tgatgaacca acgactgctt tagatgtaac aattcaagca caaattttaa
     4501 acttaatgaa aaaaattcaa gcagaacgag actcctccat tgttttcata actcatgact
     4561 taggggtcgt tgcagggatg gcagaccgtg tagcagttat gtatgcaggg aaaatcgttg
     4621 aatttggaac tgttgatgaa gtcttttata atccacaaca tccatatact tggggattat
     4681 tgaattcaat gccgacaacc gaaacagaat caggtagttt agagtcaatt ccgggaacac
     4741 caccagattt gttgaaccca cctaaaggag atgcatttgc agctcgtaat gaatttgcat
     4801 tagatattga tcatgaagaa gaaccaccgt acttcaaagt ttcagaaaca catttcgctg
     4861 ctacatggct tttagatgaa agatcaccta aggtgcttcc tccattaccg atccaaaaac
     4921 gttgggaaaa atggaacgag attgaaggga ggaaagccca atgactgaaa atcgaaaaaa
     4981 attagttgaa gtcaaaaatg tttctttgac cttcaataaa ggaaaagcta atgaagtgag
     5041 agcaattgat aatgttagtt ttgacattta tgaaggtgaa gtgtttggat tagttgggga
     5101 atctggatca ggaaagacaa ccgttggacg ttcaatttta aaactttatg atatttctga
     5161 tggcgaaatt acctttaatg gagaagtgat ctcacatctg aaaggtaaag cattacatag
     5221 ttttcgtaaa gacgctcaga tgatttttca ggatcctcag gctagcttaa acggccgtat
     5281 gaagattcgt gatatcgtag cagaaggttt agacatacat aaattagcta aatcaaaatc
     5341 agatcgtgat agtaaagttc aggccctatt agatcttgta gggttaaata aagatcattt
     5401 aacacgttat ccgcatgaat tttcaggtgg acaacgacaa cgtatcggga ttgcacgtgc
     5461 tttagcagta gagcctaaat ttatcattgc tgatgaacca atatctgcct tagatgtttc
     5521 aatccaagca caagttgtta atttaatgca aaaattgcaa agagaacaag gattgactta
     5581 tttgtttatc gcacatgatt tgtctatggt aaaatacatt tcagatcgta tcggtgttat
     5641 gcattggggg aaactgttag aagttggaac atctgatgat gtttataaca aaccaatcca
     5701 cccttatacg aagagtctat tatcagctat tccagaacca gatccggaga gtgaacgtca
     5761 acgtgttcac cagccgtata atccagctat tgagcaagac ggacaagaac gtcaaatgca
     5821 cgagattaca cctggtcatt ttgttttatc tacaccccaa gaagctgaag aatataaaaa
     5881 acaaatatta taaaagatgg ctagcccatc ttttattttt ttactaataa aataaattta
     5941 ggtatggaat tttaaactct atgttattgg tgttagccaa caggaaact
//
LOCUS       DATROY010000112         5916 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.113, whole genome shotgun sequence.
ACCESSION   DATROY010000112 DATROY010000000
VERSION     DATROY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            9..686
                     /locus_tag="VBD11_001647"
     CDS             9..686
                     /locus_tag="VBD11_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6954585.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            679..2124
                     /locus_tag="VBD11_001648"
     CDS             679..2124
                     /locus_tag="VBD11_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6954586.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2131..4290
                     /locus_tag="VBD11_001649"
     CDS             2131..4290
                     /locus_tag="VBD11_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954587.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            4430..5842
                     /locus_tag="VBD11_001650"
                     /pseudo
     CDS             4430..5842
                     /locus_tag="VBD11_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2002 a          860 c         1138 g         1916 t
ORIGIN      
        1 aacaaaaaat gaaagaccta ttacgaaata gtctagagca aagtggaaat ttaagttttc
       61 aagatatgat tttacatatt cttgtagcag ctttattgag tgtagttatt tatgtttcct
      121 atgcttatac gcatagtgga actgcctata gtaaaaagtt taatgtttca ttaatgacat
      181 tgacggtctt gactgcaaca gtaatgaccg ttattggtaa taatgtagcc ttgtcattgg
      241 gtatggtcgg tgccttgtca gttgttcgtt ttaggacagc cataaaagat tcaagagata
      301 cagtttatat tttttggacc atagttgttg gtatctgttg tggtgtcggt gactatgtgg
      361 tagctgcatt aggaagtagc gttatcttta tcttattatg ggttatggga cgtgttaaaa
      421 acgagaatcg tatgttattg attgtgaagt gcgatagaac actagaagtt gatttagaag
      481 gaattttctt ccaatatttt gacggaaaag ctgttcagcg tgttaaaaat tcaacaacta
      541 atactattga aatgattttc gaaatctcta gaaaagatta cgataagcaa ctccatgtag
      601 ataatcagtt aactgaaaaa gtgtaccaat tgggaaatat tgattatttc aacattgtta
      661 gccaaagcga cgaaatcaat gggtagactt atgagaggga aggttattta cggcacaacc
      721 cttataggtc tttttctatt cttatttttc tatttttgga ttcctaagca tcacatcgag
      781 agaatacatc atcatcgtat aaagcaggta gatgcgaaga gtgatttaac aggatttaaa
      841 acccatttgc ccattatcag cattgataca aagcaacaag ttattcctct tgttacaaaa
      901 gaaggcggaa aatatgtcaa agctagggat aatattaatg ttgatatcga attacgggat
      961 tctccaagta gatcacatca tttatcagaa aagccgagaa ttaggacaaa agggttaata
     1021 tcatatagag gaaattcctc tcgttacttt gataagaagt cattgaaagt taagtttgtt
     1081 actaataagt taaaggaaaa gaagcatcga ttagcaggaa tgcctaaaga atcggagtgg
     1141 gtattgcatg gtccctttct agacagaaca ttattaagaa attatctgag ttataatatt
     1201 gctggtgaga ttatgtccta tgccccaaac gttcgctact gtgagttatt tgtcaatggt
     1261 gagtatcagg gagtatatct agcagttgag aatatcgaac aaggtgagca acgagttccc
     1321 attgaaaaaa gtgataaaaa gttgcataaa acaccttata tcgttgcttg ggatcgtgaa
     1381 cataaggcaa agcaaaagtt agataactat gtacattata cacaccagtc tggtatctca
     1441 gctcttgatg tgaaatatcc aggtaaacaa agattgacat caaaacaatt agagtttatt
     1501 aataaggaca tcaatcatat tgaaaaagtc ctttattctt atgatttttc acaatatcct
     1561 aaatatattg atagagaatc atttgcaaat tattttgtca tcaatgaatt ttttagaaat
     1621 gtggatgcgg gtaaattttc aacctatcta tataaagatc ttcgtgatag agcaaaacta
     1681 gtagtttggg attttaataa tgcttttgat aatcaaattg aaggacgtgt tgatgaggca
     1741 gattttacct tgacggatgc accgtggttc aatatgctca ttaaggacaa agcatttata
     1801 gacctggttg ttcatcgcta taaagaatta cgaaagggag ttctagcgac agaatattta
     1861 tctaattaca tagacgagac aaggcatttt ttagatccag ctattgatcg aaattataaa
     1921 aaatggggct atgtctttga tttaaagaat actgatccaa ggaattattt gattcctact
     1981 gaacgcaatg tgacaagtta tcacaaatca gtagaacaat taaaagattt tatcaaaaag
     2041 cgtggcagat ggatggatag aaatattgag accttgtatc aatatagtgc accatcgaaa
     2101 aatactaata ctttactgga gtagaacatt atgaaaaaat atctaagtat tgtagccctt
     2161 ttaactattt tgtttagctc tatttattat ttgtattact ttgatggtag tttgtattta
     2221 ccaaagggct tattaaaaga aaatacaaga actaactttg ttgttaaagg tgatactgta
     2281 cttcacaagc ccaccaataa accttttgtt gttaaaggag tagacgttga gtcttcctta
     2341 gcgggttatc atcacaacga ttttcctatt actcaaaaaa cgtatcgtga atggttccat
     2401 ttaatttcca acatgggggc aaatactgta agagtcaagg taccgatgaa tgttgcattt
     2461 tacgatgcct tatatcacca caacaaagaa tcaaagaggc cactgtattt gttgcaagga
     2521 atacgtatag attcttatcg caataatgct tctataacag cttttaatga taattatagg
     2581 gggtatttaa aacgagaagc aaaaggcgtt gtggatattc tccatgggcg taagcaagta
     2641 tggaatactg attttggtag ccgtcattat cattatgatc ttagtccttg ggtacttggt
     2701 tatgtcgtag gggatgattg gaatagtggt actgtcgctt atactaatca tcaagagaaa
     2761 aaaacgcaat ataaaggacg ttattttaaa acttctgtgg cagctaatcc atttgaggtc
     2821 atgctagctc aagtaatgga tgaattgaca cattatgaga cagctaaata tggttggcaa
     2881 catttgatta gtttttcaaa ctcaccaaca acagaccctt ttcattatcg aaaaccattt
     2941 gaggcacagg ctcctaaata cgtacaacta aatgtagaaa atattcaagc taattcaaat
     3001 gttaaagcag gtatgtttgc agcatataaa gctattgatt tccatcctcg atacaaggat
     3061 tatctattat ttgataaaga gaatatcagt aaagaagata gacaaaagat taaagaactt
     3121 tctttgtcac agggatacgt taaactgcta aatgcttatc acaaaatccc tgttctagtc
     3181 acgggttatg gctattcgac agcgagaggt attgcccaaa aagaaattga taaacgtcct
     3241 ctgccgatta atgaaaaaga acaaggtcag cgtttactag aagattatga atcttttata
     3301 tcatccggta gttttggagc gactatcaat gcatggcaag acgattggaa tgcaagggcg
     3361 tggaatacat ctttcgccac aaataaacat agtcaattcc tatgggggga tgcacaagta
     3421 tttaatcaag gttatggttt attaggcttt aaaaacgcaa aacatcatta tcaagttgat
     3481 ggtaaaagag gcaaaggaga gtggaaacat cctctgatga ctagtgcaac aggagatgac
     3541 ttatatgcta gcagtgatga aagctatctc taccttgcga ttaaaacaaa acctgaaaaa
     3601 ctaaaagaaa aacgattatt accaatagat attacaccaa aatctggtag tagaaaaatg
     3661 aatggtagta aggtcacatt ttctaaatct agtgactttg tattgtctat tgatccaaat
     3721 ggcaagtctg aattatttgt ccaagagcgc tataatgcct taaaagcgaa ctatcttcga
     3781 cagcttaacg gtaaagattt ttatgctttc ccaccaaaga agaacagtag taattttgag
     3841 cagataaata tggtattgag aaatacaaag attgttgaag acatggaaaa agtaaaagca
     3901 acagagaggt tcttaccaac tcatcctact ggtcttctca aaacaggaac aactgatagg
     3961 caccaaaaaa catttgattc acaaacagat atttcgtttg gaaaggactt tatagaggtc
     4021 agaattccgt ggcagttgtt gaatttttct gatccatcat ctcaaaaaat tcacgatgat
     4081 tactttaaac attatggtgt gaaggagtta gaaattgaga gcattgcttt aggattaggt
     4141 gctaatagca aagaaaacac actgataaag atggcagatt atcgtttgaa aaattgggag
     4201 agacccgata ccaaaacctt tttaaaagac tcctattata gtattaagaa agaatggtct
     4261 aaagaaagag agagaacata tggtacataa tattttatat ttttacattt tgatttgctt
     4321 agcaattatt gtttttaaca ttcactatat cttaagacaa cgattagata agcttagttg
     4381 gcttcgtatt cggaaaaagg atataaatat caaataaagg ggattttcta tggcattatt
     4441 ttttaccttc ctaaaggtat ctcaagtcat tttcttagta ttattgattg cctatgccag
     4501 ctttttagtg atttcattag tcattggagg tgttcatctc tatgaaaaaa atgagcgcaa
     4561 aaaaagttta actagaaata aagaaaaaat tccaatttca attattgtac cagcctataa
     4621 tgaatcaaca accattgtga gttcgattga ttcgttattg caccttgatt atgaagcgta
     4681 tgaaattatc gttgtggatg acggttccag tgacaatact tcagatgttc tcaaggaaga
     4741 gtttgcatta atgaaaatct cgaatacgat tgattcaatt attgctactc aaacttgtaa
     4801 agatgtcttt cagagacagg ttggtaaggt taaactgaca ctcattgtta aagaaaatgg
     4861 tggcaaaggg gatgccttaa atatggggat caatgctgct aattacgatt attttctatg
     4921 tctagatgca gattctatgt tgcaggtaga ttctttgagt caaattagca aatccataca
     4981 ggtagatccg acggttatag cagtgggagg gcttgtccaa gttgcccaag gtgttaagat
     5041 tgagcagggg aaagtagcat cttatcgttt gccttggaga attattcctt gtgcacaagc
     5101 acttgagtat gattcatcat ttttaggagc tcgaatcttt ttagactatt taagagctaa
     5161 tttaattatt tcgggggctt tcggtctttt taagaaagac cttgttaaag cggtgggagg
     5221 atatgacacc cagactctag gagaagatat ggagttggtg atgaaattac atttcttctg
     5281 tcgtaataat aatattcctt atcgtatttg ttatgaaaca gatgctgttt gttggagtca
     5341 agcaccaacg aatttagggg atttacgtaa acagagacgc cgatggtatc ttggcttgta
     5401 ctaatgttta aaaaaatata aaagtatttt tgccaattat cgctttggag ctgtaggatc
     5461 aatttcttat atttactata ttttatttga attactgacc ccttttatag aatgttttgg
     5521 tattgtcatt atatttttgt ccttattatt taatcaatta aacatcccat tctttatatc
     5581 attggtgagc ctttatatct tttactgtgt tttaattaca ttaagctctt tcttacaccg
     5641 tatttattct cagcaactag taataggaat tttagatatt gttaaagtat tttatattgc
     5701 tgtttttagg tatcttatcc tacatcctgt attaactttt gttaaggttg caagtgttat
     5761 tggctataaa aataaaaaaa tggtttgggg gcatattacg agagagaaac aaatgaagga
     5821 ttttgaaaca agtgttactt agaagttata gcgattcata tatataaaat aatcactaaa
     5881 agagcgtaat tatctacgct cttttagtga ttatca
//
LOCUS       DATROY010000113         5868 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.114, whole genome shotgun sequence.
ACCESSION   DATROY010000113 DATROY010000000
VERSION     DATROY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5868
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            67..752
                     /locus_tag="VBD11_001651"
                     /pseudo
     CDS             67..752
                     /locus_tag="VBD11_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            749..2137
                     /locus_tag="VBD11_001652"
     CDS             749..2137
                     /locus_tag="VBD11_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6954588.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            2230..2838
                     /locus_tag="VBD11_001653"
     CDS             2230..2838
                     /locus_tag="VBD11_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6954589.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(2928..5429)
                     /locus_tag="VBD11_001654"
     CDS             complement(2928..5429)
                     /locus_tag="VBD11_001654"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6954590.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            5645..>5868
                     /locus_tag="VBD11_001655"
     CDS             5645..>5868
                     /locus_tag="VBD11_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6954591.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQLRESTKDCLDCPLLRKAPYVCNGCPK"
BASE COUNT         1889 a         1172 c         1074 g         1733 t
ORIGIN      
        1 ttatatttga tataacataa acagctagct aaaagtcaaa ttataactat gggtattgag
       61 gtatacatga caaaaataat ttatttcgct gatgatgaaa agaatattcg tgacttggtg
      121 gttccttttt tagaacatga tggtttcact gttcgagcat ttgaaactgg agaccttctc
      181 ttagaagcct ataaaaacca aaaacccgat ttagttatat tagacataat gatgcctgga
      241 actaatggtt tagatgttat gaaaagcatc cgccaatatg ataatattcc tattatcatg
      301 ttaacagcta gagattctga tgttgatttc attacggctt ttaatttagg aactgacgat
      361 tacttcacca agcctttttc acctataaaa cttagtctac atgttaaagc attgttcaaa
      421 cgccttgatg aaaaagcaat caaaaatgac actcagtatc agttctagat ttaattctgg
      481 atactgaaaa gcgaatagcc ctattatcaa atgaagaaat gcccttaacc aagacagaat
      541 ttgattttct tttagtatta attgaaaaac ctgaaactgc tttttctagg gaaacactgc
      601 ttaatcgtat ttggggattt gatgatattg aaagcagagc agttgatgac actattaaac
      661 gattacgtaa aaaacttaag caataccaca gtcaggttag tatcaaaact gtctggggtt
      721 acggcttcaa attaatcaaa gaggacaaat gaaaaagaaa agtttaatta gcaatcttat
      781 ccttattaat agcatcactc tatttcttgg tttttacctc atctattttt ctgtcaacta
      841 tgtcattaaa gactatgtcc atcaaatgac aaggaattcc atgcaggcga atttcaccat
      901 tttagatagt atctataaca acaagaaaat accagatgaa aatatggtta accaagatag
      961 catctttgtt tggtcttact atgctatttt tgaccagcat tatcgtatga aatatggcac
     1021 tgataatcat aaaaaagaga gccatgatat tattcgctat cttacccaaa aaagattgtg
     1081 gcaaatttcc aaggaaaaag atggtatgtt tataaccatc aagaaaaaaa cctattatgt
     1141 aatgaccaaa gactactctg gtatccttgt tgatggtagc ataaaaaaag ctcctaaagc
     1201 acagtcgcaa ctttttcatg tgattaactt ttcagacatt acttacaccc agcatctaat
     1261 tactaaaatt aatcattttc ttatagttat cttagtacta acctatattc ccatgctatt
     1321 tattatgagg aagactttca ctggaattag agaatctatt caatcagtac aaacttatat
     1381 ttccagcctt tggaaaaatc aaggcaatca tcaatctagt caaaaagaaa ttgtcttttc
     1441 tgatttcgat cctcttctac ttgagagcca agaaatggca aatcgaatct accaggcaga
     1501 agagagccaa agaaacttct ttcaaaatgc ctcccacgag ttacggacac ctttaatgtc
     1561 catccaagga tatactgagg gagttcaaga gggtattatt gatgccgagc tcgcacactc
     1621 tgttattctg caagagagca aaaaaatgaa acagctcgta gacgatatta tcttactctc
     1681 taaattagat agtaacttaa gtgatcaaaa agatgaattt tctttaaatg aattacttaa
     1741 tagtattatt gcttatttca aacccctagc taataaacaa aagattagta tcacttatag
     1801 accagacaag catgaaaagt tactaaaagg taacgaagag ttgattcaac gagctatcaa
     1861 taatatcctt agtaacgcac tacgctatgc tgtttctcat attgaaatct cttacacaaa
     1921 tcaaaaacta actatctcaa acgacggacc agccatttca aaagaagact tgccttatat
     1981 tttcgatcgc ttctataaag gacatggcgg ccaaacaggt attggacttg caatgactaa
     2041 agaaattatc aagcaacatc atggtaatat catcgctgag agtgattcga cctcaacaac
     2101 ttttacgatt tatttcaatt gccacaaaat tgcctaactt ttcctacaaa aattcaaaga
     2161 agttattgct tttttaacct ataataccta tagatcaaaa agttattcta aaagaaagtg
     2221 agtttttata tgttacattc taaaaaaata cattccttat cgcttattgc cgttctctct
     2281 ttagcaacat atacgagttt acaaccaaat catgtagcgg ctgaacaatc acaaaaaaca
     2341 tcaactgttc atatgagtca aaaaactatt gaacataagt taaaagttgc agataaagaa
     2401 gctgctcctc tctacgctaa aatcgaccat atccaacgac atattgaagt caaaaaagca
     2461 aaagatttaa aagttattga attgtatatt aacaaagata tcaaccaact agagaagcaa
     2521 aataaacgtc tactaactaa attctatact tctattgata atcaaacatg ggatagcaca
     2581 agtgaagtca aaaaattgat tgataagaca accctatcca ctaacgaaaa agatagatta
     2641 aaattatatt ttgaacaacg tgcttacctt gagacaaggt tgaacgaccg ctatcaaaaa
     2701 tttgataact ctattgaaaa ccaaaataaa gaactaaaaa tattaacgtc aaaaatagaa
     2761 aaaatctatc aaaaacatgg tattacaaaa gaggtattaa aaacttacta tgctaaaaaa
     2821 acagtacgag ctgactgatt tagctaaaaa tagaaaaatc ccaaaagttc ataaactttt
     2881 gggattttat acgcgtaaat atcactattt ctagtaaaat ctttatctta tttcaccaca
     2941 atattaacca gtttgtttgg tactgcgatg actttgatga tgtctttgcc tgcgatttcg
     3001 gcttggactt tttcgttagc aagggcaact tcttgtaatt cttcgcgtga aaggtcttta
     3061 gcaactacaa gtttagcttt gaccttacct ttgatttgga caacgatttc aatttcgttt
     3121 tcaaccaatt tgctttcgtc ataacttggc catggtacgt aagagattga ctcacctgag
     3181 gctgtcaaga cttgccagag ttcctcaccc aagtgtggtg cgaatggtgc aatcaattga
     3241 acaaagcctt tggcatagtc gctaaagagt ttgtcttcct tgttggctgc attgacaaag
     3301 accatgagtt gagcgatagc tgtgttgaat ttcatttggt caacttgctc agtaactgcc
     3361 ttaacggttt cgttgtaaac cttgtcaagg gctccgctat tttcttcagt aatctcttta
     3421 gtcgtgatca aacggtaaac acgatccaag aacttacgtg agccttcaag accttcttca
     3481 gaccaagcga ttgaagcgtc aagtggtccc atgaacatct cgtaaacacg aagggtgtca
     3541 gcaccgtact gttcaacaac atcatctggg ttgacaacgt tcttaagtga tttagacatc
     3601 ttagctggtg cttgttcaag ctcttcccca gtttccacat ggaagaagct accatcacgt
     3661 ttttcaacct tatcagttgc cacaagagca ccacggctat cgcggtagct tgttcccaaa
     3721 atcatccctt ggttaaagag tttttggaat ggctctttgg taggtacgac accgagatcg
     3781 taaagaacct tgtgccagaa gcgagcgtag agaaggtgaa gcacggcgtg ctctgcacca
     3841 ccgacataga tatcaactgg caaccattgt ttgaggagtt cctcgtcagc caatttttca
     3901 gtattgtgtg gatcgatgta acggagatag taccagcttg aacctgccca ttgtggcatg
     3961 gtgttagtct cacgacgacc tttaacacca tcttcacgag tcacttccaa ccagtctgtc
     4021 aagttagcaa gtgggctctc accagtacct gaaggacgga tatccttagt tactggaaga
     4081 accaatggca attcactctc aggaaccgct gttgatgtac catcttccca gtggatgatt
     4141 gggattggct caccccagta acgttgacgg ctgaagagcc agtcgcggag acggtaagtg
     4201 actttctcat taccaacacc ttctgcttca agccattcga ccattttggc aatagctgac
     4261 gctttatcga gaccatctag gaagtctgag ttgatgtgaa gaccgtcttc tgtaaaggca
     4321 gcctcttcaa cattaccacc ttcaagaact ggaatgatgt ctagcttaaa ttgttttgcg
     4381 aattcccagt cacgttcatc gtgggctgga acagccatga tagcacctgt accgtagctg
     4441 gcaaggacat agtccgcaat ccaaactgga atttctttac cattaactgg gttaatggca
     4501 taggcacctg tccagacacc tgttttttct ttggcaaggt ctgtacgagc aaggtctgat
     4561 ttgagacttg cttgacgttt gtattcggca acggcttctg cttggtcagc tgttgtaata
     4621 gcatcaacca aggcatgctc aggagcaaga actgcgtaag ttgcaccaaa aagggtgtca
     4681 ggacgtgttg taaagacagt gaagtcctta tctgtatcct taaccttgaa ggttacatta
     4741 gcaccagttg atttaccaat ccagttgcgt tgcatgtctt tgattgattc tggccagtca
     4801 acttcttcca agtcttctag caagcgctct gcataggcag tgattttgag catccattgg
     4861 cgcattggtt ttctgacaac tgggtagcca ccacgttctg atgtaccatc tggaagaacc
     4921 tcttcgttgg cgatagctgt tcccaattct tcaacccagt taactggaac ttcagcttcg
     4981 taagctagac ctttttcata aagcttggta aagatccact gtgtccactt gtagtagtta
     5041 gggtcagttg tgttaacttc acgatcccag tcgtatgaga aaccaagagc attaatctgg
     5101 cgtttgaagt tagcgatgtt ttcagctgta aactcagctg ggtcattacc agtatccata
     5161 gcgtactgct cagcaggaag tccaaaagca tcccaaccca ttgggtggag gacattgtga
     5221 ccttgggcac gtttgaaacg gctgagaata tccgttgctg tatagccttc tgggtgacca
     5281 acgtggagac cagctccaga tgggtatggg aacatatcta gggcataaaa cttaggtttt
     5341 gatgcgtctg ttcctgtttt aaaggtgtga ttatctgccc aaaaagcttg ccatttgggt
     5401 tcgatttctt tgtgattata gaatgtcatc gcacttctcc atgtcaaaaa agtatagtcc
     5461 tcttattata acacgaaaaa cagcaataaa aaaggtgaaa ataccctcaa cagcaggttc
     5521 tctccccaca gtaatttctc aaagtaagtt ccgtctgccc tccaaaaccg gaagttagtg
     5581 tgaaaaggat ttttatggtg gaattaagtc tgagacgttt gggttagaaa tgagtttatc
     5641 catcatgaca aaacacaaac acctaactct ctcagaccgt aatgacatcc aatctggttt
     5701 ggatagagga gaaaccttta aagccatcgg gcttaatcta ctgaaacacc ctactactat
     5761 cgcaaaagaa gtcaaacgaa ataagcaact aagagaatca accaaagact gcctagactg
     5821 tccgctacta agaaaagctc cctatgtttg taatgggtgt ccaaagag
//
LOCUS       DATROY010000114         5839 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.115, whole genome shotgun sequence.
ACCESSION   DATROY010000114 DATROY010000000
VERSION     DATROY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..935
                     /locus_tag="VBD11_001656"
     CDS             <1..935
                     /locus_tag="VBD11_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6954592.1"
                     /translation="CYASLRFGAINFKTSDLITVLKNPLKNSNAQDVIFDIRLPRIIA
                     AILVGAAMSQAGAIMQGVTRNAIADPGLLGINAGAGLALVVAYAFLGSMHYSTILIVC
                     LLGSVISCLLVFTLSYTKQKGYHQLRLILAGAMISTLFTSVGQVVTLYFKLNRTVIGW
                     QAGGLSQINWKMLIIIAPIIILGLLISQLLAHQLTILSLNESVAKALGQKTQLMTAFL
                     LLIVLFLSASSVALIGTVSFIGLIIPHFIKLFIPKDYRLLLPLIGFSGATFMIWVDLS
                     SRIINPPSETPISSIISIVGLPCFLWLIRKGKNL"
     gene            932..1933
                     /locus_tag="VBD11_001657"
     CDS             932..1933
                     /locus_tag="VBD11_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6954593.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(1963..2616)
                     /locus_tag="VBD11_001658"
     CDS             complement(1963..2616)
                     /locus_tag="VBD11_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6954594.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(2676..3611)
                     /locus_tag="VBD11_001659"
     CDS             complement(2676..3611)
                     /locus_tag="VBD11_001659"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6954595.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            complement(3728..4516)
                     /gene="pflA"
                     /locus_tag="VBD11_001660"
     CDS             complement(3728..4516)
                     /gene="pflA"
                     /locus_tag="VBD11_001660"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6954596.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            complement(4584..>5839)
                     /locus_tag="VBD11_001661"
     CDS             complement(4584..>5839)
                     /locus_tag="VBD11_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6954597.1"
                     /translation="ASEMALVSLNRSKVEQKAEEGDKRYRRLLDVLENPNNFLSTIQV
                     GITFISLLQGASLSASLGHVISGWLGNSATARTAGSIIALIFLTYVSIVLGELYPKRI
                     AMNLKDRLAIVSAPIIIFLGKIVSPFVWLLSASTNLLSRITPMTFDDADEKMTRDEIE
                     YMLTNSEETLDAEEIEMLQGIFSLDEMMAREVMVPRTDAFMIDINNDAQSNIEGILSQ
                     NFSRVPVYDDDKDRVVGVLHTKRLLEAGFKTGFDTIDLRKILQEPLFVPETIFVDDLL
                     KALRNTQNQMAILLDEYGGVAGLVTLEDLLEEIVGEIDDETDTAEQFVREIDENIYIV
                     LGTMTLNEFNDYFETELESDDVDTIAGYYLTGVGSIPNQEEKVAYEVDSKDKRITLIN
                     DKVKDGRITKLKVLLSDIEQNIEKD"
BASE COUNT         1806 a         1127 c          924 g         1982 t
ORIGIN      
        1 gttgttacgc cagcttacga tttggtgcta tcaatttcaa gactagtgat ttaataactg
       61 ttttaaaaaa tcccttaaaa aactctaatg ctcaggatgt tatttttgac attagactac
      121 cacgcataat tgcagctatc ttagtaggtg cagctatgtc acaagcaggt gctattatgc
      181 agggagtcac tcgaaacgcc attgctgatc ctggactact aggtattaac gccggagctg
      241 ggttagccct tgttgttgca tatgcttttt tagggagcat gcactatagt acgattctca
      301 ttgtctgctt actagggtct gtcatttctt gtcttttagt cttcacactc tcttatacaa
      361 agcaaaaggg ttaccaccaa ctacgactca ttttagctgg agcgatgatc tccacacttt
      421 ttacgtctgt tggacaagtg gttacattat actttaaact caatcgaact gttatcggtt
      481 ggcaagcagg tggtttatcc caaattaact ggaaaatgct aatcataata gcacctatca
      541 ttattttagg tttacttatt tcacaactcc tagcacatca attaactatc ttgagcctta
      601 atgaatctgt ggccaaagca ttgggacaaa aaactcaatt aatgactgcg tttctattac
      661 ttattgtttt gtttttatcg gcttcatcag tcgcgttgat aggaaccgtt tctttcattg
      721 gattgattat tccacatttt attaagcttt ttattcccaa agattatcgt ctgttattac
      781 ctttaatagg attttcaggg gctactttca tgatctgggt agacttatca tctcgtatta
      841 taaatccccc ttcagagaca ccaattagtt ctataatcag tatcgtaggg cttccatgtt
      901 tcctatggtt aattagaaaa gggaaaaatc tatgattcaa aaaaataaag ctccttttgt
      961 tttaatatca tctgtcatta tcctactatt attaatacta gtatcaattt cacttggcta
     1021 tgctaatact tccgttattg acgttttaaa actaataagt ggaaaatctg atgatgcctt
     1081 cctttttatc attactaaca ttcgtttacc tcgtattatt gtctgtatct ttgggggagc
     1141 ttctttagga attgctgggt tgctattaca gacattaacc aaaaatccac tagctgattc
     1201 cggtattctt ggtatcaatg ctggagctgg attagtaatc gctctaacca taggaacatt
     1261 taatgttagc aatccaacta tattatactt tttaccccta tttgctatgt ttggagggtt
     1321 ggtaactatt tttcttgtct atcttatgtc atacagaaga aatcacaata ttagccctac
     1381 acgattaatc atcactggta tcggaatatc tacaatgatt tcaggcgtta tgatttctat
     1441 tattagtcag tctaataatc aaaaaatgga tatgatcgtt gaatggctta gtggaaaaat
     1501 tacaattagt tcctggacta ccattatcac ttttatcccg attctcattt tgttatgggg
     1561 attagcatat agtcgcagtc gccaccttaa tattatgaac ctaaacgagc agactgctct
     1621 cgctctaggg cttcatttaa aaaaggaaag aatatatact ttgatgctgg caagtagcct
     1681 tgctgctatt agcgtcgtct taattggtaa tattactttt ataggcttac tcgctgggca
     1741 cttaagtaga cgactattag gaaataacca taaaattata ctacctagct gtcttcttat
     1801 tggtgctatt atcctattgg tatcagatac tataggtcgt ctcctcttag ttggtacagg
     1861 tatacccact ggattggttg tatcaataat tggtgccccc tattttctct ggttgatgac
     1921 aaaagttgac taaaaaagac tggacaaact tccagtcttt ttttatgtta agtaaatctt
     1981 taaagcttca acatcaagtt tagcatttaa acgataggta gtatcttggt ttaagctaat
     2041 atatccaagt aattctaaag cttggataaa aatatctgta cgtttttgct taacactatc
     2101 cttgcgaaga aatttcaata taaagcttga catatatttt aaagcatatt ccgaattaac
     2161 atctcctaaa agttgataaa gctttttttg ttctcttgaa agtggataac agttagttag
     2221 tttatagaaa taattagaaa gagtcaaagt gtttctttca aaatctgtct cttctacaaa
     2281 taccaagtga ttggtttggt tatctaaatt tgtcacaaat tttcgcttct gtaataactg
     2341 atagatttga ctagcagaat ctatgaaaac caaatcatct aatgctaatc catccagcga
     2401 actcagaaaa ggaagattaa ggctgtagcg tttattttgg cgaaggatat atccatgatt
     2461 aatatattct tctaacaaac ggtcaatttt agaatagtct gtaaagtggg actttatttg
     2521 tcttagtgtt acatcatcat gtatccaaag atagttaata agctccttaa aaaactcttg
     2581 tctttctaac tttcctggat taaacgttaa tatcataaaa acgctcctaa atgtatgact
     2641 aaaaaaacgg gacgcagccc gttttacatg attaattaag cattgtagct ttctgtcaat
     2701 tgaggaacaa cttgtttttt acgtgatact gccccagcaa gaaaagcgtg gttattttca
     2761 agtgtaaatt caaaagctgc ttcaacttta gccatatttg aaccgagagc taaaatctct
     2821 gaatttgaat taacaatgtc agtaatcatc aatacaaaat ccgaataacc ttctgtgaca
     2881 attgcttctt gaatagcaac ttcaatctct tcttgacgtg ctaaaatatc gttaatgtcc
     2941 acagtattaa cttgtgcaac acgaacagct tctccattta attcaaatgt tttagcatca
     3001 atatcaatta actccgctgc agtcttactt gagaggttag tacctgcttt aagcatttct
     3061 aaaccatatt cttctaagtt tacacctgca agctctgcta gttcttttgc aactggaata
     3121 tctgaagcat gagttgttgg tgatttcaaa agaagagtat ctgaaatcaa acctgataat
     3181 aataaaccag ccaactcttt gggtactgat actccatttt ccttaaacat acggtaaaca
     3241 atcgatgatg ctgagccaac tggttccaaa cgcatatata acggattggc agtctcaaag
     3301 ttagcaacac gatgatgatc aacaacacca taaactgtca catctttaat atcagaaata
     3361 gattgttgga attcgttatg gtcagttaaa atgactgtct ctacaccctc tgcttttgca
     3421 gattctacta ctcgaggtgc ttgaacacca aaataatcta aaacatatgc tgtttcttca
     3481 tttggagttc ctaaagcaac ggcctctgtg tcaagccccc aagcttcttt agctagatat
     3541 gcaaaagcaa ctgatgatcc aatagcatct gaatctggat tttggtgacc aaagactaaa
     3601 attttagaca tgtttttacc tcttctttta tattcacctt attgtagcaa aaattgaata
     3661 acttagcaaa aaatcttact aggaagccct ttgcaactaa aaaagttgaa taaaattcaa
     3721 cttaaaacta attttgaatt ctttttagat attctgtata ggattctgtt ttcatgatat
     3781 ctttagcgtt tttaacacgt tctggagttg gtggtttcac accagctaat ggatacggaa
     3841 ttcctaattc acgccatttg aactctccca tagtatgata aggcaatact tcaaatttat
     3901 cgacattatc aagtgtttgg acaaattcac ccaaacgttt taaatggtca tcaatatcag
     3961 tcaatccagg aactaaaaca tgacgaatcc ataccggagt acctctatca gataaatatc
     4021 tagcaaactg taaaatattt ttgttagact gacgagttac aattttatgt tgttctggat
     4081 caatttcttt taagtcaagg agtaccaaat cagtaacatc taacaatttt tcaaggatag
     4141 catggtattc tggtgttgct cgataagcaa aaccacaagt atctagcgtc gtatgaatac
     4201 ctagtttctt agcctcaata aagagagctg taataaagtc aattcgcagc attgcttcac
     4261 cacctgaaac tgtgatacct ccatcttttc cccaaaaatg tttataacga agagcttctt
     4321 ttagaacatc ctcaacggtg cgttcctttg aattgttagt ttccatttcc caagtatcag
     4381 gattatgaca gtattgacaa cgcattttac atccttgcat aaaaatgata aaacgaatac
     4441 ctggcccatc cacagagccg aaactttctg tagaatggat cattcctgtt acttttttat
     4501 agtctatttc tgccatttca tcaccttgta accgttttta tgactttatt atatcatttt
     4561 catgaaaaaa agactaagta atcttagtct ttttcaatat tctgttctat atcagacaat
     4621 agcactttca attttgttat acgaccatct ttaactttat cattaatcaa agtgatgcgt
     4681 ttatctttgc tgtctacttc gtaagctact ttttcctctt ggtttggaat actacccaca
     4741 ccagttaaat aataacccgc aattgtatca acatcatcac tttctaactc tgtctcaaaa
     4801 taatcattaa attcattaag cgtcatagtt cctagaacaa tatagatatt ctcatcgatt
     4861 tccctgacaa attgttcagc tgtatctgtt tcatcatcaa tttcgccgac aatttcttcc
     4921 aataaatcct ctagagttac taaaccagca acgccaccat attcatctag taaaatggcc
     4981 atttgatttt gagtgttgcg taatgctttt aaaagatcat ctacaaaaat cgtttctgga
     5041 acaaaaagag gttcctgaag tatttttcgc aagtcgatag tatcaaaacc agttttaaag
     5101 cccgcttcca ataagcgctt ggtatgtaaa acaccaacaa ctctatcttt atcgtcatca
     5161 taaacaggga cacgagaaaa gttttgtgat aaaattcctt caatatttga ttgtgcatca
     5221 ttgttgatgt caatcatgaa agcatcagta cgcggaacca taacttcacg cgccatcatt
     5281 tcatctagcg agaaaatccc ttgcagcatc tcaatttctt cagcatccaa agtttcttca
     5341 ctattcgtca acatatattc aatttcatca cgtgtcatct tctcgtcagc atcatcaaaa
     5401 gtcatcggag tgattctact caataagtta gttgaagctg agagcaacca tacaaagggg
     5461 cttactattt tccccagaaa gataataatg ggagctgaaa caatcgctag acgatctttc
     5521 aaattcatag caatacgctt aggatataat tctcctagaa caatagagac atatgttaaa
     5581 aagattagcg caataatact ccctgctgta cgggctgttg ctgaatttcc tagccatcct
     5641 gaaatcacat gtcctagtga ggcagataaa ctagcccctt gcaaaaggct aataaaagta
     5701 ataccaactt gaattgttga taaaaaatta ttgggatttt caagaacgtc caaaagacgt
     5761 cgataccttt tgtccccttc ttcagctttc tgttctactt tagagcggtt tagcgatact
     5821 aaagccattt cagatgctg
//
LOCUS       DATROY010000115         5783 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.116, whole genome shotgun sequence.
ACCESSION   DATROY010000115 DATROY010000000
VERSION     DATROY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            159..2023
                     /gene="glgB"
                     /locus_tag="VBD11_001662"
                     /pseudo
     CDS             159..2023
                     /gene="glgB"
                     /locus_tag="VBD11_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
     gene            2065..3204
                     /locus_tag="VBD11_001663"
     CDS             2065..3204
                     /locus_tag="VBD11_001663"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6954598.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            3194..4327
                     /gene="glgD"
                     /locus_tag="VBD11_001664"
                     /pseudo
     CDS             3194..4327
                     /gene="glgD"
                     /locus_tag="VBD11_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            4324..5754
                     /gene="glgA"
                     /locus_tag="VBD11_001665"
     CDS             4324..5754
                     /gene="glgA"
                     /locus_tag="VBD11_001665"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO6954599.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
BASE COUNT         1845 a          835 c         1209 g         1894 t
ORIGIN      
        1 tgaatttgag tttcttatat tttagttaat ttttcggaaa tatagagggt gaaatatttt
       61 cagatgcgtc tgtatgagga aaatgcattc ctcttttttt gtaccattga ggaaatattt
      121 ttgtcttata ttgactagta gacctgtgag gtgagtagat gtccaataaa gatgagttat
      181 atacttttgg aataggtgaa aattttcatc tccagaatta tttaggcgta catagcgaaa
      241 atggaagctt ttgtttcaga gtttgggctc ctaatgctga aaatgttcaa gttattggag
      301 attttacaga ttggcgaaat cgacctctcc aaatgaacaa aattcaagca ggagtttggg
      361 aagctaacag tttggatgcg cgcgaaggtg atttatataa atatttggtt actcgtaaag
      421 gtggtcaagt agttgagaaa atcgatccta tggcagttta tatggaaaga cgtcctggaa
      481 ctgcaagtgt tattaaagtt cttagaaata aaaagtggga agatggtctt tggatgggaa
      541 ggcgcaaacg tctgggtttt cagaagcgtc caattaatat ttacgaagtt catgctggtt
      601 catggaaaaa agatgatttt ggacatccta tgactttttc tcagttaaaa gattacttaa
      661 tcccctacct tgttgagatg aattatactc atgttgaatt tatgccctta atggcacatc
      721 cgcttgatat gagttgggga tatcaattaa tggggtattt tgcttttgaa catacctatg
      781 gtactccaga agaatttcaa gactttgtag aagcatgtca taaaaataat ataggagttc
      841 ttgtagattg ggttccagga cactttattc agaatgacga tgctttagcc tatttcgatg
      901 gaactgctac ttatgagtat caaaatcatg atcgtgcgca taactatcga tggggagctc
      961 tgaactttga tttaggaaaa aatcaagtac aatccttttt gatttcgagt gccttgttct
     1021 ggattgaaca ttatcatatt gatggtattc gtgttgatgc ggttagtaat atgttatatc
     1081 tcgattatga tgaaggacca tgggaagcta accaatttgg ggataatcgt aatttggaag
     1141 gatatcactt tttaaggaaa cttaacaaag taattaaaga gcgtcatcct aatgttatga
     1201 tgattgctga agagtcaaca gcttctaccc caataactaa agacttagag tctggaggat
     1261 tgggttttga ctttaaatgg aatatgggct ggatgaatga tatcctgcgt ttttatgaag
     1321 aagatcctct ctataggcaa tatgatttta atttagtgac ttttagtttt atgtatatat
     1381 tcaatgagaa ctttgtacta gctttttcac ataagtagta catggtaaaa agagtatgat
     1441 gcataaaatg tggggtgacc gttataatca atttgctggt ttacgtaatt tatatgcata
     1501 tcaaatgtgt catcctggga aaaaacttct ctttatggga agtgagtttg gtcaattttt
     1561 agaatggaaa tataatgatc aactagaatg ggaaaactta aatgatgata tgaaccaaaa
     1621 gatgcaacgc tatactaaac aattgaatca attttataaa gatcataagt gcctatggcg
     1681 tattgatgat tcctttgacg ggctagaaat tattgatgcg gataataaat ctgaaactgt
     1741 tttatctttt atacgaaaag atgataaagg cgatttgtta ctttgtgtct ttaatatgac
     1801 tcctgtagaa cgccctaact ttactattgg tgttccgcaa gcgggtattt atgaagaagt
     1861 tttaaatact gaaatggaag agtttggagg tgtttggaaa aaccacaatc ctgtaacgaa
     1921 aacacaagtg gctacatgga aagattatga tcatacttta agttttacat taccagccct
     1981 tggggcaagt gtttggagaa taaaacgtcg gcttcgtaaa taagtctgca atttagtaac
     2041 ctcattttga aaggaaagaa taatatgaaa aatgaaatgt tagcactcat tttcgtcgga
     2101 ggacaaggca ctcgactcgg aaaattgact cagagcattg cgaaacctgc tgttcaattt
     2161 ggaggtcgtt accgtattat tgactttgcg ctttcaaatt gtgcgaattc aggtattaat
     2221 aatgttggag ttattactca gtatcaaccg ctggaactca atactcatat tggaaacgga
     2281 tctagctggg gactagatgg tatcgattcg ggagtaactg tattacagcc atattctgca
     2341 acagaaggta atcgctggtt tcaaggtacc agccacgcta tctatcaaaa tattgactac
     2401 attgatcgta ttaatccaga atatgtactc attctctctg gggaccacat ctataaaatg
     2461 gactatgatg acatgctaca gacgcataaa gataatttag caagcctaac agtcgctgtt
     2521 ttagatgttc ctttaaaaga agcaagccgt tttggaatta tgaatactga ttcaaacgac
     2581 cgtattgtcg aatttgaaga aaaacctgaa catcctaagt caacaaaagc ttcaatggga
     2641 atatacattt ttgactggaa gagattacgg acagttttaa ttgatggtga aaagaacggc
     2701 atagacatgt cagactttgg taaaaatgtt ataccagcat accttgaatc gggtgaacgt
     2761 gtttatacct ataattttga tgggtattgg aaagatgttg gtactattga atccctttgg
     2821 gaagctaata tggaatatat tggagaagat aacaaattac atagtcgtga tcgttcatgg
     2881 aaaatctatt ctaaaaattt gattgcacca cctaatttca tgacagaaga tgcaaatgtt
     2941 aaagattctt tagttgtaga tggatgcttt gttgcaggaa atgttgagca ctccatttta
     3001 tcaactaatg tccaggtaaa accaaatgct attattaaag actcctttgt tatgagtggc
     3061 gcgactatcg gtgaaggtgc aaagataaat cgagctatta tcggtgagga tgctgttatt
     3121 ggtgatggtg ttgtgattga tggaagtaaa gaagtagaag ttattggtta taaagaagta
     3181 gcgggggttc caaatgaaga ttgataaata tactgcgatt ttaggaaata ctgttagtta
     3241 cccagatatg cacagtttga cggataatcg tccgcttgct tctctacctt ttgatggtaa
     3301 gtatcgttta attgattttc aattatcctc tttagctaat gcaggcgtta gaagtattta
     3361 tggtattttt cgtgggaata atgttcgctc tgtttttgac catattagaa gtggtcgtga
     3421 gtggggctta ggtacactgt tgagtcacta ttctttagga ttctatgata cagacgatga
     3481 tactcgtaca gtcaatgggg attattacga ttaaatttta acttacttga ggcgttcggg
     3541 tagcgaccag acgatatata tgagttgtga tatgctttgt aatattgact taagtcaagt
     3601 aatccacttg cataatgcaa atggtgtgca agccaccgtg gtttataaaa aaatgccgag
     3661 atcagctatt gcagaggcta atcaagtctt gaccattgat gaatctgatc atgtagtgag
     3721 tctaggtaaa gctgtagata atgacgataa ccaaaaaatg tctgctgaga tttatgttat
     3781 cgatacacct tggttaatcg aaaaaatgga agaggaagct caaaataatg aaccacgtaa
     3841 actccgcttc ttacttcgtg atttaattgt tgaaagtaat gctttggctt ttgaatacac
     3901 tggttatttg tcaaacattt cttctattaa atcctattat gatgctaaca tggacatgtt
     3961 aacaccaaat aaattttatt cgctcttctt ttcaaaccaa aaagtttata ctaaggtgaa
     4021 aaatgaggaa gctacttatt ttgataaaca atcaaatgtc tctaattcgc aactagccta
     4081 tggaagtatt attaaaggtt atttagacca ttctatcgtt tctagaaatt gtctccttga
     4141 aaaagggact cgagtagtca atagtattat ctttccaaaa gtaaaaatcg gagaaggggc
     4201 aaccattgaa aatactatta ttgacaaatg cgttaaggtt gcatccggag ttacattgaa
     4261 aggtagttta gataaaccgt tagtgattcc taaattttca gagattaatg aggatattat
     4321 acaatgaaaa taatgtttgt tgcggcagag ggtgctccct ttgctaaaac aggtggtttg
     4381 ggagatgtga ttggcgcact cccaaaatca cttagtaaaa aaggtcatga tgtcgctgtt
     4441 gtcatgccat attatgatat ggtagaccaa aaatttggag accaaataga aaacctcatg
     4501 tatttctata cagatgttgg ctggcgtcat caatatgtag gagttaagag actttctcaa
     4561 gataatgtta cgttctactt tattgataat caatattatt tttatagagg tcatgtctat
     4621 ggtgattggg atgatgggga gcgttttgct tatttccaat tagctgcttt agagttgatg
     4681 gagaaaatag attttattcc cgatgtctta cacgtccatg attatcatac agcaatgatt
     4741 cctttcttat tgaaagagaa gtaccactgg attcaggcct ataataatat aagagcagtt
     4801 tttactattc acaatattga atttcaaggg cagtttggtc cagaaatgtt aggagattta
     4861 tttggagttg gcgctgagag atatgaagat ggaactctta gatggaataa ttgtcttaat
     4921 tggatgaaag cagcaattct ttactcagat agagtgacaa cggtatcacc aagctacgca
     4981 aatgaaataa aaactccaga atttggtaaa ggtttagacc aaattatgcg tatggaagca
     5041 ggtaaattat ctggaatcgt aaatggtatt gacagcgatt tgttaaatcc agaaacagat
     5101 gcttttttgc catatcattt ttcaaaaagc aacttagagg gcaaaattaa aaataagttg
     5161 gctttacagg aaaatttggg cttgcctcaa gataaaaatg taccattaat tggtattgtt
     5221 tcacgactta ccgatcaaaa aggatttgat attattgctt ctgaattgga caatatgctt
     5281 caacaagata ttcaaatggt tattttagga acaggctatc accattttga agaaacgttc
     5341 tcttattttg ctagtcgtta ccctgaaaaa ctatctgcta atattacttt tgacttagga
     5401 ttagcgcaac agatttatgc ggcttcagat atcttcatga tgccaagtgc ttttgaacct
     5461 tgtggtctct cgcagatgat ggctatgaga tatggtagct tacccttggt acatgaagtt
     5521 ggaggattaa aagatactgt ggtagccttc aatcaatttg atggtagtgg tacaggtttt
     5581 tcatttaatc atttttcagg ctattggtta atgcaaactc taaaactagc attagaagtt
     5641 tataatgatt atcctgaagc gtggaaaaag ttacaatggc aagctatgtc aaaagatttt
     5701 tcatgggata cagcatgtgt tgcttacgaa caactgtatc agcaactaca ataatcactt
     5761 tattttagtg attagttgat aag
//
LOCUS       DATROY010000116         5778 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.117, whole genome shotgun sequence.
ACCESSION   DATROY010000116 DATROY010000000
VERSION     DATROY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1116)
                     /locus_tag="VBD11_001666"
     CDS             complement(<1..1116)
                     /locus_tag="VBD11_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6954600.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYS"
     gene            complement(1386..2948)
                     /gene="guaA"
                     /locus_tag="VBD11_001667"
     CDS             complement(1386..2948)
                     /gene="guaA"
                     /locus_tag="VBD11_001667"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6954601.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3161..3859
                     /locus_tag="VBD11_001668"
     CDS             3161..3859
                     /locus_tag="VBD11_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6954602.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            3973..5307
                     /gene="trmFO"
                     /locus_tag="VBD11_001669"
     CDS             3973..5307
                     /gene="trmFO"
                     /locus_tag="VBD11_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6954603.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVSLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIYKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            5385..>5778
                     /locus_tag="VBD11_001670"
     CDS             5385..>5778
                     /locus_tag="VBD11_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5613 domain-containing protein"
                     /protein_id="HEO6954604.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKF"
BASE COUNT         2014 a          988 c         1057 g         1719 t
ORIGIN      
        1 tgaatattta agaataaatg gcgcaataat agttgacagt ataacaacaa taacaagctc
       61 ggaataagta gtgcttgaaa taattttagc agctaatcca acttgaacta taatgagagc
      121 catctcgcca cgactgacca ttccaccacc aattgttaaa gattctaatt ttgaaaaatt
      181 aaagcctcga ccgacaaagt accctggaat taattttgat aaaacagcca aagcagtaaa
      241 gattaatatc gtgtgaaggt gagacatcat tccatcgaat ttaagaggta gtgcaattga
      301 agcgaagaaa attgggataa agaaggtgta actaagaagt gaaattttat gttctacctt
      361 atcaacaaaa gaagtttgtc caatagctaa tcctgcaaaa aacgaaccaa tgatagaact
      421 cattccaact ttatcggcta aaattgatag agaaaaacag ataaggatag caagaatagt
      481 atatttctca aagaaatgaa ccttttcgat taatttgaaa agagcaggta caaggtattt
      541 catacaaata aataagaaaa caaagaataa caattgaatg ataatttgta atccgatatt
      601 agaagagctt ccatttgttg caataaagac actcagaagc aaaacagcta agacatcatc
      661 agcaacagca gcacctaaaa taatagcacc agtatctgtt tttacacgat tgtattcttg
      721 taaaacttca actgtgatag aaatactagt tgcagcaaat acgagcccgt aaaagatagc
      781 agtattgatt tggaacccaa acaagcgtgt caagaagtag aagagagcca taggaacaat
      841 aacaccagta attgcgacca gtagactggg cttaagatac ttctttaaaa gatcaaaatt
      901 agcttcaaga cccgctaaaa acatgagtaa gataacacca atttctgaca ggacatgtaa
      961 tacctgattt tgatgaacaa gtcctagcat tgcaggtccg atgatgatac ctacaagtag
     1021 ttgtccaaca acagccggta ttccaattct ttttgaaata agagttgcaa gaacgcttgc
     1081 caacaaaatg attgtaattt gaatgataat atgcattttt tcctcctaat attaataaaa
     1141 tgatatcaat catattgtac tgaatattta gaataccatt ttttaagcct aaaatctagg
     1201 aatatttctt ttctgtcttt ttcatattat tatcaagaaa atggaaaaat atactgaaaa
     1261 atattcaatt taagaataat gaacttgtaa ctatcgaact acataaaaat gatattttaa
     1321 gtgtaagtat cccttattca agaaataaga gtaaaaaaac ttagctactt aaagtagcta
     1381 agtttttatt cccactcaac agttgcaggt ggtttacttg taatatcata aacaatacgg
     1441 ttaacgtgat caacttcgtt aacaatacgt gtcgaaattt tcttaagtac atcccaagga
     1501 agttgcgcaa agtcagctgt catgccatca attgaagtaa ttgcacggat agcaattgta
     1561 tagtcataag tacgaccatc tcccataaca ccgacagaac gtacgccagt attaactgtg
     1621 aaatactgcc atacgtcacg atctagacca gctttagcga tttcttcacg aagaatagca
     1681 tctgattcgc ggactgtttc aagtttttct tcggtaattt cacccataac gcgaatagca
     1741 agaccaggcc cagggaatgg ttgacgccat acaacttcat caggcattcc aagtgcagta
     1801 ccaagagcgc gaacctcatc tttgaataat gtgttaagtg gctcaatcaa ttcaaattgc
     1861 atatcttctg gcaatccacc aacattatgg tgtgacttga ttgtttgggc tgtttctgtc
     1921 ccagattcaa taatatctgt gtagagggta ccttgagcaa ggaaatcaac gcctttaagt
     1981 ttacttgcct catcatcaaa aacgtacacg aactcatttc cgatgatttt tcgttttctc
     2041 tcgggatctt caacaccaga aagaagatct aggaagcgtt ttgaagcatc aacacggata
     2101 atattaagtc caaatttacc accaagcata tccataactt ggtcaccttc atttttacgt
     2161 aataaaccat gatctacaaa aatacatgtt aattgatcac cgatagcacg ttgtaggagt
     2221 actccgacaa cagatgaatc aacaccgcca gatagtccaa gaagtacttt acgatcgcct
     2281 acagtttcac gtatttttgc aatttccata tcaatgaagt tatccattga ccaatcacca
     2341 cgtgcaccac agatattaac agcaaaattt ttaaggatat cattgccata tactgagtga
     2401 cgaacttcgg ggtggaattg gataccataa aattgttttt cagtattttc cattgcagca
     2461 aatggacagt caacagagtc accaacgaga tggaaaccct cagggatttc agtaacagca
     2521 tcaccatggc tcatcaaaac aagttgttct tgaggagtgc cagcaaagag tgctgattct
     2581 gagcgtaggc gaagtgctga ttgaccatac tcacggtgac cagcttctcc cgcaggaaga
     2641 accttaccac cgagtttatg ggtaatcagt tgcataccat aacaaattcc taagattgga
     2701 attcctaatt caaaaatttc ttcatcgatt ccaaaagcgc catcagcgta tacggaattt
     2761 ggaccacctg aaagtacgat accaatagga ttaatatcgc gaatttcatc agccgtaatt
     2821 ttatgtgatt ttaactcaga aaatacacca aattcacgga tacgacgtgc gataagctgg
     2881 ttatattgac taccgtaatc tagaacgata attttttgga tatcattcaa tatagaaata
     2941 tcagtcattg tttccctttc tttgtagcta attttaaaaa cagctgtacc tataccattt
     3001 tagcatattt tatataaatt aacatcatta aaataattga ttaaacgctt tcttatcaag
     3061 aaatattgct tatgattttt ctgaattatt tcacgatttt agagttagaa atatatagaa
     3121 gtatggtaaa atgaagtgta gattctagga ggagagaata ttgttaccag catatattaa
     3181 aattcacgac gctattaaaa aggaaatcga taaagggact tggaagattg ggcagcggtt
     3241 acctagcgaa agagatttag cggatgatta tagtgtaagt cgtatgacac ttcgccaatc
     3301 aattacattg ttagttgaag aaggtatttt ggagagacga gttggtagtg gcacctatgt
     3361 tgctagtcat cgtgttcaag aaaaaatgcg gggaacaact agttttacag aaattgtcaa
     3421 ttctcaaggg cgtaagccat ccagtaaact aatctcattt caaagaaaac tagctaatga
     3481 aaccgaaatt caaaaattaa atttatcaca gtcagattat gtggttcgta tggaaagggt
     3541 acgttatgct gataaggtac cgttggttta tgaggtagcc tctataccag agaatttaat
     3601 taaaggtttt gaacaatcag aggttacaga acatttcttt aaaaccttaa cagagaatgg
     3661 ctacgaaatt gggaaaagcc aacaaactat atatgctaga aatgctagtg aacgtgttgc
     3721 ttcgcattta gaagttaacg caggccatgc tattttggca ttgacacaag tttcatactt
     3781 tactgatggt aagccttttg aatatgttca tgggcaatat gttggagatc gttttgagtt
     3841 ttatttagag aataactagt agtttaatga ataacgtttt ttgaagaagt agctatgtat
     3901 gttataatgg aaaaataaat tatatagaaa gatttgagga agtagttgac tactttcaag
     3961 aggtattagt tcatgtctca atcttacatt aatgttatcg gcgctggttt ggctggttct
     4021 gaagcagctt atcaaattgc taaacgtgga ataccagtta agctttatga aatgcgtggg
     4081 gttaaatcaa cccctcaaca taaaacagat aattttgcag aattagtatg ttccaattca
     4141 ttccgcggtg attctctaac aaatgcagtt agtcttttaa aagaagaaat gcgtcgtttg
     4201 gattctatca ttatgagaaa tggggaggca catcgagttc cagcaggtgg agccatggcc
     4261 gttgatagag aaggttattc tgaagctgtt actgaggaaa tctataaaca tccattaatc
     4321 gaagtaattc gtgatgaaat cacagatatc cctggagatg ctattacagt tatcgctaca
     4381 ggaccattaa caagtgatag tctggcagct aaaatccatg aacttaatgg tggtgatggt
     4441 ttttattttt acgatgccgc cgctccaatt gtcgataaaa atacgattga catgaataaa
     4501 gtttacctta agtctaggta tgacaaaggt gaagcagctt atctaaattg tccaatgacc
     4561 aaagaagaat ttatggcttt tcatgaagct ttaactacag ctgaggaagc accactaaat
     4621 tcttttgaga aagaaaaata cttcgaaggt tgtatgccta tcgaagtgat ggctaagcgt
     4681 ggtattaaaa cgatgcttta tggtccaatg aaaccagttg gtttggagta tcctgaggac
     4741 tacaaaggac cgcgagatgg tgaatttaaa acaccatatg ctgttgttca attacgtcaa
     4801 gataatgctg caggctctct ttataacata gttggtttcc aaactcatct taagtgggga
     4861 gaacaaaaac gtgtatttca aatgattcct ggtcttgaaa atgctgaatt tgtccggtat
     4921 ggtgttatgc atcgcaattc ttatatggat tcaccaaatc ttttaaacca aacctttgct
     4981 acacgtaaga acccaaatct tttctttgct ggacaaatga ccggtgttga aggttatgta
     5041 gaatcggcag caagtggtct tgtagcggga atcaatgctg tccgtcgctt caatggggag
     5101 agtgaagtag ttttcccaca aactactgct attggtgcac tgccacatta tattacacat
     5161 acagacagca aacatttcca accaatgaat gttaactttg gtatcatcaa agaattagaa
     5221 ggtccacgaa ttcgtgataa gaaagagcgt tatgaagcta ttgctacacg agctttgaaa
     5281 gatttagaaa aatttctcaa ctattgatat attcatttga ctttgccaac aagcaaggtc
     5341 aaattttcta tattgatttt taaaaatgta tgaatggagt aaatatgcac tataaagaat
     5401 tacttgagtc atccgaaaaa gcttatttgg aaactaatcg gtataaacat tttaagaata
     5461 taatcacttc agagcgttat ttttcaaact atattagcta caaaataatg cctacagtaa
     5521 gtgagttttt acaggatatg atctatttag cagatgaaca aaaagattat cagtctgatt
     5581 ttgcttttgt attttttgag gaaaatgctg aattgagcag tgaattacag agaactttat
     5641 cacaacaagg ctttgaatca gagaaacata tcatatttac aaattcatta aatcatctta
     5701 aactttttga aaaagaattt gatgacatca catttactaa acttaatgaa aattacctag
     5761 aaaaatacct aaagttca
//
LOCUS       DATROY010000117         5753 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.118, whole genome shotgun sequence.
ACCESSION   DATROY010000117 DATROY010000000
VERSION     DATROY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            44..1708
                     /locus_tag="VBD11_001671"
     CDS             44..1708
                     /locus_tag="VBD11_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6954605.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            1797..2720
                     /locus_tag="VBD11_001672"
                     /pseudo
     CDS             1797..2720
                     /locus_tag="VBD11_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            2722..3639
                     /gene="srtC1"
                     /locus_tag="VBD11_001673"
     CDS             2722..3639
                     /gene="srtC1"
                     /locus_tag="VBD11_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6954606.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            3596..4447
                     /gene="srtC2"
                     /locus_tag="VBD11_001674"
     CDS             3596..4447
                     /gene="srtC2"
                     /locus_tag="VBD11_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6954607.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            4529..>5753
                     /locus_tag="VBD11_001675"
     CDS             4529..>5753
                     /locus_tag="VBD11_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6954608.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFN"
BASE COUNT         2031 a          972 c         1178 g         1572 t
ORIGIN      
        1 gcacttacaa agaacaaagg gaaaagcgag gagagaactt ttaatgaaat tatcgaagaa
       61 gttattgttt tcggctgctg ttttaacaat ggtggcgggg tcaactgttg aaccagtagc
      121 tcagtttgcg actggaatga gtattgtaag agctgcagaa gtgtcacaag aacgcccagc
      181 gaaaacaaca gtaaatatct ataaattaca agctgatagt tataaatcgg aaattacttc
      241 taatggtggt atcgagaata aagacggcga agtaatatct aactatgcta aacttggtga
      301 caatgtaaaa ggtttgcaag gtgtacagtt taaacgttat aaagtcaaga cggatatttc
      361 tgttgatgaa ttgaaaaaat tgacaacagt tgaagcagca gatgcaaaag ttggaacgat
      421 tcttgaagaa ggtgtcagtc tacctcaaaa aactaatgct caaggtttgg tcgtcgatgc
      481 tctggattca aaaagtaatg tgagatactt gtatgtagaa gatttaaaga attcaccttc
      541 aaacattacc aaagcttatg ctgtaccgtt tgtgttggaa ttaccagttg ctaactctac
      601 aggtacaggt ttcctttctg aaattaatat ttaccctaaa aacgttgtaa ctgatgaacc
      661 aaaaacagat aaagatgtta aaaaattagg tcaggacgat gcaggttata cgattggtga
      721 agaattcaaa tggttcttga aatctacaat ccctgccaat ttaggtgact atgaaaaatt
      781 tgaaattact gataaatttg cagatggctt gacttataaa tctgttggaa aaatcaagat
      841 tggttcgaaa acactgaata gagatgagca ctacactatt gatgaaccaa cagttgataa
      901 ccaaaataca ttaaaaatta cgtttaaacc agagaaattt aaagaaattg ctgagctact
      961 taaaggaatg acccttgtta aaaatcaaga tgctcttgat aaagctactg caaatacaga
     1021 tgatgcggca tttttggaaa ttccagttgc atcaactatt aatgaaaaag cagttttagg
     1081 aaaagcaatt gaaaatactt ttgaacttca atatgaccat actcctgata aagctgacaa
     1141 tccaaaacca tctaatcctc caagaaaacc agaagttcat actggtggga aacgatttgt
     1201 aaagaaagac tcaacagaaa cacaaacact aggtggtgct gagtttgatt tgttggcttc
     1261 tgatgggaca gcagtaaaat ggacagatgc tcttattaaa gcgaatacta ataaaaacta
     1321 tattgctgga gaagctgtta ctgggcaacc aatcaaattg aaatcacata cagacggtac
     1381 gtttgagatt aaaggtttgg cttatgcagt tgatgcgaat gcagagggta cagcagtaac
     1441 ttacaaatta aaagaaacaa aagcaccaga aggttatgta atccctgata aagaaatcga
     1501 gtttacagta tcacaaacat cttataatac aaaaccaact gacatcacgg ttgatagtgc
     1561 tgatgcaaca cctgatacaa ttaaaaacaa caaacgtcct tcaatcccta atactggtgg
     1621 tattggtacg gctatctttg tcgctatcgg tgctgcggtg atggcttttg ctgttaaggg
     1681 gatgaagcgt cgtacaaaag ataactaaat aaaaggctac ttcttaagta accatgttta
     1741 agaaaagaga aatagcctta tttctctttt tgtcgttttt aaaataaagg aacatcatga
     1801 aacaaacatt aaaacttatg ttttcttttc tgttgatgtt agggactatg tttggaatta
     1861 gctaaactgt tttagcgcaa gaaactcatc agttgacgat tgttcatctt gaagcaaggg
     1921 atattgatcg tccaaatcca cagttggaga ttgcccctaa agaagggact ccaattgaag
     1981 gagtactcta tcagttgtac caattaaaat caactgaaga tggcgatttg ttggcacatt
     2041 ggaattccct aactatcaca gaattgaaaa aacaggcgca gcaggttttt gaagccacta
     2101 ctaatcaaca aggaaaggct acatttaacc aactaccaga tggaatttat tatggtctgg
     2161 cggttaaagc cggtgaaaaa aatcgtaatg tctcagcttt cttggttgac ttgtctgagg
     2221 ataaagtgat ttatcctaaa atcatctggt ccacaggtga gttggacttg cttaaagttg
     2281 gtgtggatgg tgataccaaa aaaccactag caggcgttgt ctttgaactt tatgaaaaga
     2341 atggtaggac tcctattcgt gtgaaaaatg gggtgcattc tcaagatatt gacgctgcaa
     2401 aacatttaga aacagattca tcagggcata tcagaatttc cgggctcatc catggggact
     2461 atgtcttaaa agaaatcgag acacagtcag gatatcagat cggacaggca gagactgctg
     2521 tgactattga aaaatcaaaa acagtaacag taacgattga aaataaaaaa gttccgacac
     2581 ctaaagtgcc atctcgagga ggtcttattc ccaaaacagg tgagcaacag gcaatggcac
     2641 ttgtaattat tggtggtatt ttaattgctt tagccttacg attactatca aaacatcgga
     2701 aacatcaaaa taaggattag catgggacaa aaatcaaaaa tatctctagc tacgaatatt
     2761 cgtatatgga tttttcgttt aattttctta gcgggtttcc ttgttttggc atttcccatc
     2821 gttagtcagg tcatgtactt tcaagcctct cacgccaata ttaatgcttt taaagaagct
     2881 gttaccaaga ttgaccgggt ggagattaat cggcgtttag aacttgctta tgcttataac
     2941 gccagtatag caggtgccaa aactaatggc gaatatccag cgcttaaaga cccctactct
     3001 gctgaacaaa agcaagcagg ggtcgttgag tacgcccgca tgcttgaagt caaagaacaa
     3061 ataggtcatg tgattattcc aagaattaat caggatatcc ctatttacgc tggctctgct
     3121 gaagaaaatc ttcagagggg cgttggacat ttagagggga ccagtcttcc agtcggtggt
     3181 gagtcaactc atgccgttct aactgcccat cgagggctac caacggccaa gctatttacc
     3241 aatttagaca aggtaacagt aggtgaccgt ttttacattg aacacatcgg cggaaagatt
     3301 gcttatcagg tagaccaaat caaagttatc gcccctgatc agttagagga tttgtacgtg
     3361 attcaaggag aagatcacgt caccctatta acttgcacac cttatatgat aaatagtcat
     3421 cgcctcctcg ttcgaggcaa gcgaattcct tatgtggaaa aaacagtgca gaaagattca
     3481 aagaccttca ggcaacaaca atacctaacc tatgctatgt gggtagtcgt tggacttatc
     3541 ttgctgtcgc ttctcatttg gtttaaaaag acgaaacaga aaaagcggag aaagaatgaa
     3601 aaagcggcta gtcaaaatag tcacaataat tcgaaataat aaaatcagaa ccctcatttt
     3661 tgtgatggga agtctgattc tcttatttcc gattgtgagc caggtaagtt actaccttgc
     3721 ttcgcatcaa aatattaatc aatttaagcg ggaagtcgct aagattgata ctaatacggt
     3781 tgaacgacgc atcgctttag ctaatgctta caatgagacg ttatcaagga atcccttgct
     3841 tatagaccct tttaccagta agcaaaaaga aggtttgaga gagtatgctc gtatgcttga
     3901 agttcatgag caaataggtc atgtggcaat cccaagtatt ggggttgata ttccaattta
     3961 tgctggaaca tccgaaactg tgcttcagaa aggtagtggg catttggagg gaaccagtct
     4021 tccagtggga ggtttgtcaa cccattcagt actaactgcc caccgtggct tgccaacagc
     4081 taggctattt accgacttaa ataaagttaa aaaaggccag attttctatg tgacgaacat
     4141 caaggaaaca cttgcctaca aagtcgtgtc tatcaaagtt gtggatccaa cagctttaag
     4201 tgaggttaag attgtcaatg gtaaggatta tataaccttg ctgacttgca caccttacat
     4261 gatcaatagt catcgtctct tggtaaaagg agagcgtatt ccttatgatt ctaccgaggc
     4321 ggaaaagcac aaagaacaaa ccgtacaaga ttatcgtttg tcactagtgt tgaagatact
     4381 actagtatta ttaattggac tcttcatcgt gataatgatg agaagatgga tgcaacatcg
     4441 tcaataacga tgttgtgaat ggcttactta cttatcaaat aggtgactaa tgatgattgt
     4501 gaataatggt tatctagaag ggagaaaaat gaaaaagaga caaaaaatat ggagagggtt
     4561 atcagttact ttactaatcc tgtcccaaat tccatttggt atattggtac aaggtgaaac
     4621 ccaagatacc aatcaagcac ttggaaaagt aattgttaaa aaaacgggag acaatgctac
     4681 accattaggc aaagcgactt ttgtgttaaa aaatgacaat gataagtcag aaacaagtca
     4741 cgaaacggta gagggttctg gagaagcaac ctttgaaaac ataaaacctg gagactacac
     4801 attaagagaa gaaacagcac caattggtta taaaaaaact gataaaacct ggaaagttaa
     4861 agttgcagat aacggagcaa caataatcga gggtatggat gcagataaag cagagaaacg
     4921 aaaagaagtt ttgaatgccc aatatccaaa atcagctatt tatgaggata caaaagaaaa
     4981 ttacccatta gttaatgtag agggttccaa agttggtgaa caatacaaag cattgaatcc
     5041 aataaatgga aaagatggtc gaagagagat tgctgaaggt tggttatcaa aaaaaattac
     5101 aggggtcaat gatctcgata agaataaata taaaattgaa ttaactgttg agggtaaaac
     5161 cactgttgaa acgaaagaac ttaatcaacc actagatgtc gttgtgctat tagataattc
     5221 aaatagtatg aataatgaaa gagccaataa ttctcaaaga gcattaaaag ctggggaagc
     5281 agttgaaaag ctgattgata aaattacatc aaataaagac aatagagtag ctcttgtgac
     5341 atatgcctca accatttttg atggtactga agcgaccgta tcaaagggag ttgccgatca
     5401 aaatggtaaa gcgctgaatg atagtgtatc atgggattat cataaaacta cttttacagc
     5461 aactacacat aattacagtt atttaaattt aacaaatgat gctaacgaag ttaatattct
     5521 aaagtcaaga attccaaagg aagcggagca tataaatggg gatcgcacgc tctatcaatt
     5581 tggtgcgaca tttactcaaa aagctctaat gaaagcaaat gaaattttag agacacaaag
     5641 ttctaatgct agaaaaaaac ttatttttca cgtaactgat ggtgtcccta cgatgtctta
     5701 tgccataaat tttaatcctt atatatcaac atcttaccaa aaccagttta att
//
LOCUS       DATROY010000118         5588 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.119, whole genome shotgun sequence.
ACCESSION   DATROY010000118 DATROY010000000
VERSION     DATROY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(96..1292)
                     /locus_tag="VBD11_001676"
     CDS             complement(96..1292)
                     /locus_tag="VBD11_001676"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6954609.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1477..2412
                     /locus_tag="VBD11_001677"
     CDS             1477..2412
                     /locus_tag="VBD11_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6954610.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2393..2587)
                     /locus_tag="VBD11_001678"
     CDS             complement(2393..2587)
                     /locus_tag="VBD11_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6954611.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2676..4340)
                     /gene="dnaX"
                     /locus_tag="VBD11_001679"
     CDS             complement(2676..4340)
                     /gene="dnaX"
                     /locus_tag="VBD11_001679"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6954612.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4340..4837)
                     /locus_tag="VBD11_001680"
     CDS             complement(4340..4837)
                     /locus_tag="VBD11_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6954613.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4924..5553)
                     /gene="udk"
                     /locus_tag="VBD11_001681"
     CDS             complement(4924..5553)
                     /gene="udk"
                     /locus_tag="VBD11_001681"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6954614.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
BASE COUNT         1813 a         1084 c          889 g         1802 t
ORIGIN      
        1 atttcctata cttaaccagt ttattataag attttataaa tgttctataa attatcatga
       61 gcaaaaaaag cgtaaacttt cgtttacgct ttttattatt tgctagcaat aaaatccttt
      121 aaggcctgga ctttatcaac tcgctcccat ggcaaatcga tatcagtacg tcccatatgc
      181 ccataggctg ctgtttgtcg ataaataggt cgtttgaggt ccaacatatt aataatgcct
      241 gctggtcgca aatcaaatat ttggcgtaca gctgcctcta ggtctgcttc tgctataaca
      301 cctgttccaa atgtatctac acgcacagag acaggctgag caacaccaat tgcataagca
      361 agttgtactt ctactttttt agctaaatca gctgcaacaa tattttttgc aatataacga
      421 gctgcataag atgctgaacg atctacttta gtagcatctt tacctgaaaa tgcaccacca
      481 ccatgacgag aataaccacc ataagtatct acaataatct tacgacctgt tagtcctgaa
      541 tctccttgag ggcctccaat aacaaagcgc cctgtaggat tgataaagaa ttttgtttgg
      601 tcatctaaat aatggcttgg aataacttca ttgataactt tctcaatcac gtccttatgg
      661 agttggtcat tagttacatt aggatcatgc tgtgtcgaaa taactactgc atccacacga
      721 attggttgat cattatcatc atattcaacc gttacttgag attttgcatc tggtcgaaga
      781 taggtgagtt ccccagactt acgtagatct gtcaatttct taactaactg atgagctaaa
      841 gaaattggca atggcattaa ctcaggtgtt tcatcaacag caaacccaaa catcaaacct
      901 tggtcacctg caccaattaa atctaaagga tcttgttcaa gagaaccacg tacctcaaga
      961 gcttcattga ctccctgtgc aatgtctggc gactgttcaa cgagtgacgg atgcactccg
     1021 acagattcag ctgagaagcc atattcagct ttatcatagc caatttcagc gattgtgtta
     1081 cggacaactc ggttaatatc aacataggct gtcgttgata tctcgccaaa tacatgaacc
     1141 gaacctgtat aaacagctgt ttcggctgct acgtgggcat ctggatcttg ctctaaaatt
     1201 gcatctaaaa tcgcatcgga aatttggtca gcaatcttat cgggatgtcc ctcagatact
     1261 gactcagacg tgaaaagttt acgttctgac ataaaaatgt cccccctaaa atgtaaaatg
     1321 tgtcgagtta caaagaacta gcctctaggc taccttttcg ggactcatat agctattgta
     1381 ccactatttc atcttttgtg aaataagtta ccaataaata aaaaattatc tctattttct
     1441 ttagtctttt ttctcgattt atactatact agatttatga aaacctacga aaaaatttat
     1501 caaatactat caaaaaataa caactttatt agcggagaaa ctatggcaaa tcaacttaat
     1561 atttcccgca cagctatttg gaaaggtata aaaacgttag aagaattagg cctagagatt
     1621 gagtctgtca ctaacaaggg ctaccgattg gtatcaggtg acatattatt accagaacaa
     1681 ctcgaacaag aaattggtat taaggttagt cttaataata atagtgcttc aacccaacta
     1741 gatgctaaaa tggggataga aagcaaatta aagactccac accttttttt agctcctaat
     1801 caaaaaaaag ctaaaggacg ttttgataga cctttcttta catcaaatca aggcggcatc
     1861 tatatgtctc ttttattaca acctaatgta ccgattgaag atataaaacc ttatactgtc
     1921 atggtagcct ctagtgctgt aaaagctatc tcacggttaa caggaattac tcctgaaatt
     1981 aagtgggtca atgatattta tttagataat aaaaaaatag ctggtatttt aaccgaggct
     2041 atcgcttctg ttgagagtgg attggtaacg aatgttatta taggattagg tatcaatttc
     2101 tatatcaaag agttccctag ggcgttaact aagcgcgcag gtagtctctt tacagagcaa
     2161 ccgaccataa ctagaaatca attgattaca gaaatctgga atctcttttt caatattcca
     2221 ctagaggatc acttaaaggt atatagagaa aaatccctag tcttagataa aaccgttagc
     2281 tttatggatg gtcaaacaat gtattctggg aaagctattg atattactga taaaggatat
     2341 ttagtggttg agttagatga tggtcagttg aaaacacttc gcagtggcga aatcagtctt
     2401 tcttcttggt agatttgata agctcttttg tgaattttga aatcgcatac accttttgaa
     2461 gatctcgaag taataaaact ccccaaaaaa tagcaaaaat ccaggagcca tccataacgg
     2521 attgattgaa aaaataagtt tcaaaagagc ataaaagcgc cataaaaata aattgtcgtt
     2581 ttgtcatatc accattataa cagaatctac taaataatta aaaaacttat caaaaaaata
     2641 gttgcctgtt acgggcaact atttttagtt acgttttaat cttcaacgat tgctattcgt
     2701 tctgctagat aagaaaagtc ttctggaatc tgatgattaa cttcaggttc tgtttccgtc
     2761 ttttgagctt tcattttctt tgcaaaatcg ctccgaattt gattaaaatc atttctaggt
     2821 acagctaaaa tattaggtga aaaacctgct gctttactca taatattacc aaatatatca
     2881 ttgagatcgg tacgtttcat agcttgttca gcattaaagg ccgcatcaaa tgccaaaata
     2941 gcattctcgc tattagctaa gacaggttca gatcccatta ataaggctct atcttgagcc
     3001 gtaatattat ctaatatctc gttccaagca ctctttaaag cttctaaata ttcacgtgaa
     3061 cgttggctat ctacaaccgt ttcttccata atggtcaaaa tctttgtacg gtcaactttg
     3121 aatgtgaatg tcttattatt aacctttact ttttgagttg atgaactttg atctgctcga
     3181 gagagttggc tcatttcgtt ttttaacgat tttaactctc gacgaagact atctaattct
     3241 gctgtcacat ctgcgggtat gtttgacgaa tttttttcta ccatttcaga caattgaatg
     3301 gtcatcatct ctgcataaac tttaggatgg ctaccatttt ttatctcagg aagaacagat
     3361 gtcactttat caatcatttc aaataaaata tcttgtttaa catcaagatt ggccataaaa
     3421 gtatcacttg aatgactatt ttcaccacct gcttgaacaa caagtaaatc tcttaagtac
     3481 atcaataaat ctgttgcaaa acgactcata cttttgccac tatcaaagat tacttcaagt
     3541 ttagcgagtg cttcagttgt atcgtgagcc aatatattcg atacataatc atctatagca
     3601 gataaggaaa tgctccctgt aatttcttct gctacatcta aagaaatgtg gttatctttc
     3661 gctaaactca aggcttgatc gagaattgat aaagcatcac gcataccacc ttcggctcgt
     3721 ctagcgacta gtgtcagagc atccaaatca tatgaaatag cttctttatc taaaatctgt
     3781 gctaaatgat ctcgaatagc taataattta atcgctttaa attcaaagcg ctggacacga
     3841 gataagattg ttgcagggat tttatggagt tctgttgttg ctaatataaa aacaacattt
     3901 tctgttggct cttcaagtgt tttcaatagt gcattgaagg cacctgtaga caacatatga
     3961 acttcatcaa taatataaac tttataggtt gccctacttg gagcataagt tgatttgtcc
     4021 ctgatatcac gaatttcatc gacaccatta tttgacgcag catcaatttc aatgacatcc
     4081 tctaaactac catttgtaat atctcgacaa atatcacaat gattacaagg ttcgccatta
     4141 acttgatttg ggcaattcat cgcttttgca aatattttag cagcacttgt ttttccagtt
     4201 cccctaggtc ctgaaaaaag gtaagcatgg ctaatttttt tgctggacac agcttgcttt
     4261 aaagtagttg aaataacact ttgtccaacc atttcatcaa atgtttggct acgatatttc
     4321 cgataaaggg cctgatacat tacttttcaa ctccaaacat atccaaattc caaatcgtat
     4381 gttctactag aatacctaca aatttttcta agtattcttg atcaatctca tcataatctg
     4441 ctactaaaga agaatctaaa tctagaactc ctagaagttt gccattttta aacatgggta
     4501 ctacgatttc actcatagct tttgaatcac aggagatata gttagcatgc tttgtaacat
     4561 catcaacaat cagcgtctta gcagtttgtg cagattcacc acaaacacct tttcctaaag
     4621 taatatgcac acatgataca ccaccctgaa aagggccaag aattaactcc tctccatcaa
     4681 ataaataaaa gcctgtaaat acagaatttg gaagcatagc atttagcata gctgaagcat
     4741 ttgataagtt ggcaagagca tttgtttcat ctgagaatag agcttgcgct tgtagtaata
     4801 ataattgata attttcgatt ttcttagact tgttcatagc acttattata gcataatttg
     4861 aaagttacta aaactaaata accatcctat ataaaacaaa aaaataaaca caaaccattg
     4921 tgtttatttt gcttcattta agatgctagc cactttggta ttgattaaat caatagcgac
     4981 aatattactt actccttctg gaattacaat atccgcataa cgctttgttg gttcgataaa
     5041 ttgatgatac ataggtttta caacttctgt atactgttca ataatactat ctaaacttct
     5101 atcgcgctct tccatatcac gcttaatacg acgaataata cgaatatcat catctgtatc
     5161 aacaaataat ttgatatcca ttaaatcacg caaacgctga tcttctaata ccaaaattcc
     5221 ctcaacaata atgacatcct gaggttcttg gcggatagtt ctatcactac gggtatgttt
     5281 tgtgtaatca taaaccggaa tatcgaccgg acgcccctca atcaattcgt tcaattgctc
     5341 aatcatcaaa ttggtatcaa aagcaagggg atgatcataa ttggttttta cacgttcttc
     5401 aaaagtcaaa tgactttgat ctttatagta agaatcatgt tcaatcatga taattttttg
     5461 atctgggaaa tttgataaaa tcgcccgaga aacactggtt ttaccaccac cagatccacc
     5521 tgtaacacca ataataatag gttttttacg catttgtcac tccaaattaa atttatcctc
     5581 tatctatt
//
LOCUS       DATROY010000119         5426 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.120, whole genome shotgun sequence.
ACCESSION   DATROY010000119 DATROY010000000
VERSION     DATROY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            81..1223
                     /gene="tgt"
                     /locus_tag="VBD11_001682"
     CDS             81..1223
                     /gene="tgt"
                     /locus_tag="VBD11_001682"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6954615.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            1329..1640
                     /locus_tag="VBD11_001683"
     CDS             1329..1640
                     /locus_tag="VBD11_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954616.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            1647..2186
                     /locus_tag="VBD11_001684"
     CDS             1647..2186
                     /locus_tag="VBD11_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6954617.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            2325..3101
                     /locus_tag="VBD11_001685"
     CDS             2325..3101
                     /locus_tag="VBD11_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6954618.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            3101..3607
                     /locus_tag="VBD11_001686"
     CDS             3101..3607
                     /locus_tag="VBD11_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6954619.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARISH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            3642..3727
                     /gene="ffs"
                     /locus_tag="VBD11_001687"
     ncRNA           3642..3727
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBD11_001687"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3879..5228
                     /locus_tag="VBD11_001688"
     CDS             3879..5228
                     /locus_tag="VBD11_001688"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6954620.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         1740 a          861 c         1095 g         1730 t
ORIGIN      
        1 aattaataca aagtattaca tttagatatt gattagcact aaagcccatg actgtttttc
       61 atggaagcaa ggagaaaacg atgactgatc atccaataaa atatcgatta attaaacagg
      121 aaaaacacac aggagccaga cttggtgaaa taatcacacc acatggtaca tttccgacac
      181 caatgtttat gcctgttggg acacaagcaa ctgttaagac acaatctcct gaagaactaa
      241 aagaaatggg ttcaggaatt attctatcaa atacctacca tttatggtta cgtccaggtg
      301 atgaattgat tgctaaagct ggaggtttac ataaattcat gaactgggat caggctattc
      361 taacagacag tggaggcttt caggtttatt ctttggctga tagtcgaaac atcactgaag
      421 aaggagtaac ctttaaaaac catttaaatg gtgcgaaaat gtttttatca ccagaaaaag
      481 ctatttcaat tcaaaataac cttggttctg atattatgat gagttttgat gagtgtccac
      541 aattttacca gccttatgat tatgtaaaaa aatcaattga gcgaacaagc cgttgggcag
      601 aacgtggatt aaatgcccac cgccgtcctc atgatcaagg actatttggt attgttcaag
      661 gggcagggtt tgaggattta cgtcgtcaat cagcacgaga tttagtatcg atggattttc
      721 caggttattc aattggaggg cttgcggtcg gagaaacaca tgatgagatg aatgcagttc
      781 ttgattttac tgttccaatg cttccgaatg ataagcctcg ttatttgatg ggagttggtg
      841 caccagatag tttaatagac gccgttatcc gaggagtaga tatgtttgac tgcgtcttac
      901 ctactcgtat tgctcgaaat gggacatgta tgacaagtca aggtcgttta gttgttaaga
      961 atgctaaatt tgcagaagat tttacgcctc ttgatccaaa ttgtgattgc tatacttgta
     1021 aaaattatac acgcgcttat atccgtcact tattgaaagc agatgaaact tttggtattc
     1081 gtttgactag ttaccataat ctttatttcc ttgtcaatct tatgaaagat gtcagacaag
     1141 ctattatgga tgataatctt ctagagttcc gtcaagattt tatggagcgc tatggttatg
     1201 gcacgaataa ccgtaatttt taaaaaagtt cctggtaaaa cagggctttt tttgatctgt
     1261 ttgttaaccg cgttttaaaa aaagttgaca atagaaaaac ttttgtataa tatgttatag
     1321 gaggaatcat gatgcaagag tactttggta ttggtcttga tagtagcagc cgctgttacc
     1381 actatcatac taaattggat attgtcgctt taaaatgtgc tgtttgtcaa aaatattatg
     1441 cttgttataa atgtcatgat gcactcgagg agcattgttt tgcagcaaca aaatctgatg
     1501 aaatgtttcc tgtcttatgc ggttcttgtc gtcagatgtt gacattaaaa gagtataaaa
     1561 ctggtttttg tccttattgt cgtatgcttt ttaatccaaa ttgtcaacgc catgattcta
     1621 tttatttttc gaaggagtaa tttttaatgt ttcacacaaa aagcctaatc cagatggcta
     1681 tgatggctac tttattggtg gtacttggtt tcattcctgc tctaccactt ggtttcattc
     1741 cggtccctat cgtcttacaa aacctagggg ttatgatggc tggtgttatt ttgggcggaa
     1801 gaaaagggag tttatctatt ttgctcttct ttcttgtcgg tttagttatt cctgtattct
     1861 caggtttacg ttcgactata cctgtattga caggaccaac agctggttat gttattgctt
     1921 ggttgtttgt tcctttgctt atctcatatg gtgtaaaatt agtaaataaa aagaatttta
     1981 tagctatttt ccttattgta tggctagcag gggtcctttt tgtagatgta gcaggagcag
     2041 tttggctggc atcttatatg aacatcccat tagataaatc acttcttcaa aacttagtat
     2101 ttctcccagg agatacaata aaagccatta tagctgctgt tgttgctgtt aaatataaag
     2161 atagtttttt cttgactgaa aaataatcaa gattgctata ttttagatgg actagtagat
     2221 ctaaaaattt agatttacta gtcttttttg tgcataaaat ctaaaacagt tattcttaac
     2281 aaaatttggt ataatagcat taaatcagta aggaggggat tatcatgaaa ttaacaacgt
     2341 taggttgttg gggtggttat ccttataaag atggaggaac cacaagtttt ctattaactg
     2401 gagaaaataa ttttcactta ttaatagatg cgggaagtcg ttcaatcaat gagttagaaa
     2461 aagaaataag cccgttggat ttggatgcag ttatcatcag tcattatcat ccagatcatg
     2521 tagcagattt gggtgtgttg tatcattatc gtttgctata ccctaaagat aaatgggaac
     2581 ccaaagtatt gccagtttat ggacataatg aggatgatta tgagttttct aaattgacat
     2641 tacctaacgt tgctcaagga attgcttatc ataccaatgg agttgagcaa gttggtccct
     2701 tcaatattac gtttatcaaa acagtccacc cagtcgtctg ctatgccatg aggattacag
     2761 aaagagaaac tggtcaagta ttagtcttta caggagattc tgggtatttt gctgaccttg
     2821 taccttttac aaaaggcgca gatttatttt tagcagacgt ttatctttac gagggaaatg
     2881 aaaatcatat tgcacatttg actagtaaag aagcaggtga gattgcttat aacgctaaag
     2941 ttaagaaatt agttttaaca catgttccgc agtttccggg agaaggaata aaccccgaaa
     3001 atcatttgga agaacttcga ctacagtctg aaaagtatgc aggtggcatc tctgttgatt
     3061 tagctcagcc acataaatct tgggacttag gaaaactata atgcagaaat ttacccaaga
     3121 agaaaaagaa tggtttatga cagaagctct aaaggaggca aaatcctcct tagagaaaga
     3181 agaaattcct ataggttgtg tcattgtaaa agatggtcat attattggaa gaggacataa
     3241 tgctcgtgaa gagtttaata aagctatttt acatgctgaa atcatggcta ttaacaatgc
     3301 aaatgagaag gtagggaatt ggcgcttatt agatactact ttatttgtta ctgtagaacc
     3361 ttgtgtaatg tgtagtggag ctattggctt agctagaatt tcccacgtta tatatggcgc
     3421 taagaatacg aaatttggag cagcgggaag tctctatgat attttaagtg atagccgact
     3481 taatcataga gttgaagttg aaaccggcat tttagaagat caatgcgcta aaataatgca
     3541 agatttcttt cgaaaaggac gacaacgcaa gaaagaagct aaactaagtg aaaaaactaa
     3601 gatttgatag cttgcaataa aatattagtt ttgttataat atctttggag caacatttac
     3661 tcgtgaagtg ggtcagggga ggaatccagc agccctaagc gatgctaaat gtgtgctctt
     3721 tttattttca aaagtcaaag tgtttttgga aataattatg aaagtttcat aaaaattggt
     3781 agcgtttcaa aaaatatgaa aatacagtta aaaaaccttg aaaaaaaact cacaaaagag
     3841 taagatgata gtgaaacata attgttagga gaaataaaat gacacatatt acatttgact
     3901 attcaaaagt cttgggtcaa tttgtaggcg aacacgagtt agactaccta caaccacaag
     3961 taagtgcagc agatgctttc cttcgtcaag ggactggtcc tggatcagat tttctcggat
     4021 ggatggacct cccagaaaac tatgacaaag aagaattttc tcgcattcaa aaagccgctg
     4081 aaaagattaa atcagatagc gaagtactcg tggttattgg tattggtggt tcgtaccttg
     4141 gtgcaaaagc agcaattgac tttttgaata atcattttgc taatttgcaa accgcagaag
     4201 aacgtaaagc gcctcagatt ctttatgctg gaaattctat ttcatctact taccttgccg
     4261 atttagttga atacgtccaa gataaagaat tctcagtaaa tgtcatttca aaatcaggta
     4321 caacaactga accagcgatt gctttccgtg tatttaaaga acttctagtt aaaaagtacg
     4381 gtcaagaaga agctaataaa cgtatctacg ctacaactga taaagttaaa ggtgccgtaa
     4441 aagttgaagc agatgctaac aattgggaaa catttgtagt tcctgataat gttggtggtc
     4501 gtttctcagt actgacagct gtaggtcttc ttccaattgc tgcttcagga gcagatatta
     4561 ctgctcttat ggaaggcgca aatgctgctc gtaaagattt atcttcagat aaaatctctg
     4621 aaaacattgc ttatcagtac gctgcagttc gtaatgttct ttatcgcaaa ggttatatca
     4681 cagagatttt agctaactac gaaccatcat tacaatactt tggtgaatgg tggaaacaat
     4741 tagcaggtga atcagaaggt aaagatcaaa aaggtatcta tcctacatca gcaaacttct
     4801 caactgatct tcactcactt ggtcaattta ttcaagaagg ttaccgtaac ctctttgaga
     4861 cagttgttcg cgttgagaaa cctcgtaaaa atgtgactat tcctgaatta accgaagatt
     4921 tagatggtct tggttatctt caaggaaaag atgtagattt tgttaataaa aaagcaacag
     4981 atggtgtgct tcttgctcat acagatggtg gggttccaaa tatgtttgta acgcttccta
     5041 cacaagacgc ttacactctt ggttacacta tttacttctt tgagttagca attggccttt
     5101 caggttatct taactcagta aatccatttg atcaaccggg ggtagaagca tataaacgta
     5161 atatgtttgc tcttctaggt aaacctggat tcgaagagct tagcgctgaa ttgaatgcac
     5221 gtctttaaaa atatctaact aaaaagttcc tcaaagagag gaacttttta gttttgttgt
     5281 aggataacta aaaacccact agcactggta gcgatagtgg gttggaaaaa gggttgagaa
     5341 aagttattaa cttttctcag gagatttatg aaaaaggaaa ataatttagg ataatatttt
     5401 cctaacggct aggataaacc gcttat
//
LOCUS       DATROY010000120         5402 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.121, whole genome shotgun sequence.
ACCESSION   DATROY010000120 DATROY010000000
VERSION     DATROY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(207..731)
                     /locus_tag="VBD11_001689"
     CDS             complement(207..731)
                     /locus_tag="VBD11_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6954621.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            863..1726
                     /locus_tag="VBD11_001690"
     CDS             863..1726
                     /locus_tag="VBD11_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6954622.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            1811..2665
                     /locus_tag="VBD11_001691"
     CDS             1811..2665
                     /locus_tag="VBD11_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6954623.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            2775..3802
                     /locus_tag="VBD11_001692"
                     /pseudo
     CDS             2775..3802
                     /locus_tag="VBD11_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            3818..4207
                     /locus_tag="VBD11_001693"
     CDS             3818..4207
                     /locus_tag="VBD11_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6954624.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            4217..4612
                     /locus_tag="VBD11_001694"
     CDS             4217..4612
                     /locus_tag="VBD11_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6954625.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            4640..4957
                     /locus_tag="VBD11_001695"
                     /pseudo
     CDS             4640..4957
                     /locus_tag="VBD11_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            5044..5328
                     /locus_tag="VBD11_001696"
     CDS             5044..5328
                     /locus_tag="VBD11_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6954626.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGNVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
BASE COUNT         1831 a          865 c          972 g         1734 t
ORIGIN      
        1 aagacaatat agtaatcgaa aaatattcat tttgcttatg gtgacatcaa caatcccaaa
       61 gaaactcttt catcaagggg acttttttat ctcccgaatt atactcattt aatctaataa
      121 tttcaactcg ctatattaga caatcaatcc attcatcacc tctgataacg ctaatgaaat
      181 aagagaatct cccaactctc tataatttac ctctctacca tagttaaata atattttagt
      241 aaatcttcaa cagtcatttt ctgtctttga tgaagccacc actttaaggt ctcgataaaa
      301 ctggataaaa gaaattgttt taagaaatct tcaggaatgt ccacctttgt aatatactcc
      361 tcacgcaatc tagggtaaac atcgtgttct aattcacttc taaagcgtaa taaaaaatag
      421 ggatcgtcag ataataataa cgtagcgata ctatcttgat tttgttcaaa atgttttaag
      481 atatgaacta aatactcttc aaacgtaaca tctctaccct gcttaaataa gtggtgaaaa
      541 aggtcttcac agagctcctt gagtaacact tctttgcttt catagtgaga gtaaaaagtt
      601 gatcttccta catttgcaag tccgattaca tcttgaaccg ttattttaga ataatcattt
      661 tcctgtagca agcttataaa agcattataa atcgctactt ttgttttctg aatttgtcta
      721 tcttttacca tataggacac tttctaagtt ttgttcagta ttgaacaagt gttctttttt
      781 gtttctagca attctctttt atctcctaga taatagaaga aagaatcaca ctacaatacc
      841 agtataacaa aaggagaaaa atatgacatc aaacaaaaaa gtggctatcg cttttatatt
      901 aaatatcagc ttttccgtcc ttgaattcat ctttggaagt ctattcttct caggagctat
      961 cttagccgat gctgttcatg attttggtga tgcaatagct attggcatct cagctacttt
     1021 agaaaaaaaa tctaagaaag atgaagatac gatattttca ttaggttata aacgttttag
     1081 ccttttagga gctcttatca caagtctaat tcttataagt ggatctatct tagtcatgat
     1141 agaaaatatt ccaaaattgt ggcatcctac tcctgttaat tatcacggca tgtttattct
     1201 agctgtcatc gctattatca ttaatggtct agctagtttt attctccatt ctggtcaaag
     1261 taaacatgag gaaatattaa gccttcattt tttggaagat attctagggt ggttagctat
     1321 tattgttata tctcttatct taaattggaa accgttgtat atccttgatc cattattatc
     1381 agttgccata tctactttca tcttatctaa agcacttcct aaattactat caaccctgaa
     1441 acttttttta gatggtgttc cagattccat agattacgct gcattacatg atgaacttaa
     1501 gggactttcg caagtaagat ctatcaatca actcaatatc tggtcaatgg atggtattga
     1561 taatcgagca atcattcact gttgcttaaa tcaattgata tctgaaaaag attgtaaaag
     1621 agctattcga acgatttgcc aacattataa gataaacgat gttactgttg aaatcgatta
     1681 ttctttaaga gaacatcaaa atcattgcaa accattaaaa aactaacctg agtgttagtt
     1741 ttttccttga cttggagtta actctaagtg ttaagattag gtcaatacta atgaaagaag
     1801 gaaaataaag atgacagata caatgatatt aaatgacaat cataaaatac caactttagg
     1861 tttaggtacg tggatgatag ataatgatag ggtagctcaa gttgttaaag atgctatcga
     1921 attaggctac cgccatattg atactgcaca agcatacaac aatgaaaaag gggtcggtgt
     1981 agggattaaa gaatctggta ttacacgtga atctttattt gttacgacta aaattgccgc
     2041 tgaacataaa gattatgaaa gtgctacaaa atcaattgat cgatcactag aagctttagg
     2101 gcttgattat attgatttaa tgattattca tagtccacag ccttggaagg aatggcgtga
     2161 aacagataaa cattttgacc aaggaaattt agaagcttgg agagctttag aggatgctca
     2221 aaaatctggt aaggtaaaat ccatcggtgt ctcaaacttt ttagaaaaag acttagaaaa
     2281 tatcttaaaa aatggttatg ttaaacctgc tatcaatcaa atcttagctc acatcggaaa
     2341 tacacctttc gacttgattg attactgtca aagtaaaggt attcaagttg aagcttattc
     2401 accaattgca catggacaag cattgaaatc tgatggtatt caaaaaatgg ctgaaaaata
     2461 tggggttagt gttgctcaat tatgtattca atatttacta caattaaacc tgatcgtttt
     2521 accaaaggct tctagtaaag aacatttaca gtctaatcta gactttgact ttgtaattag
     2581 tgacgaagat atgtcgatat taaaatcact tatgtttgac gattatgggg aattttctaa
     2641 cttccctgtt tttagtggaa aatgaaacca tatgcggatt tttacagatg tttaattttc
     2701 aaaattttta agtttttctt gacttgaagt taactctaag agttataatg aagttataga
     2761 aaagaggtaa ggttatgaaa gtagctacat ttattgaacc tggcaaaatg gttataactg
     2821 atacaccaaa accagtcatt gaacaagaga cagatgctgt tatcaaaatt gttagagcct
     2881 gtgtttgtgg ttcagacttg tggtggtatc gtggtatttc aaaacgtgaa agtggttctt
     2941 ttgcaggtca tgaggctatt ggtatcgttg aggaagttgg tactaaagta actgacgtgt
     3001 caaaaggtga ttttgttatt gttcccttta cacatggctg tggccaatgt ccgtcttgta
     3061 aggctggatt tgatggaaat tgtacaaatc atcaagctga aaaaaatgta gataccaagg
     3121 tcaatatcta cgttatacta atgcaaactg ggcattggtt aaaattcctg gtcaaccttc
     3181 tgactatgat aatgagacac ttaactctct tctcacctta tcagatgtaa tggcaactgg
     3241 ttatcatgct gcagaagtta aagaaggtga tacggtagtt gtcatgggag atggtgctgt
     3301 tggactctgt ggtgttattg ctgctaaaat gttaggtgct aaccgtatca ttgcaatgag
     3361 tcgtcacaaa gataggcagg aactagcatt gacttttggt gcaacggata ttgtcgaaga
     3421 acggggtgac gaagccgtta aacgtgtttt agatttaacc aatcaagcag gtgccgatgc
     3481 tattttagaa tgcgttggta cagagcagtc tgttgataca gctacccaaa ttgctaggcc
     3541 tggtgctgtc ataggacgtg ttggtatccc acaaaatcca gacatgaata ccaataattt
     3601 attctggaaa aatatcggcc ttagaggtgg cattgcttct gtaacaacat ttgataagtc
     3661 tgtcctttta gatgctgttc taactcataa aattaaccca ggcttagttt ttacaaaatc
     3721 ctttgtatta gatgatattc aaaaggctta tgaagcaatg gataaacgtg acgctatcaa
     3781 atccttagtg attgttgact gaatatcgat gttaaaaatg catatcaaag aagttagtaa
     3841 aaagactggc attacgtcag ataccattag gtattatgag agaattaatc ttattccccc
     3901 tattcatcgg agtgaatcag gaattcgtga gttcacacaa aacgatattg atattttaga
     3961 atttgtccgc tattttagag gcgcaggtgt ttccgttgaa agtctcattg attatattca
     4021 tttggttgaa aaaggcgatt ccacaatccc agctaggcta gaaattttaa aagataaacg
     4081 caacaacctt gaagataggc ttcaaggcct ccaagaagca cttaatcgtc taaatcacaa
     4141 aatagacaat tatcaaaaag ttcttgttcc tagagaacaa gaactttttg atccaacaac
     4201 gaaatgagga aatcttatgc aagaaacaac taagtctatc tttcctaagg gtgaaaaaaa
     4261 tccatatggt gaattcttta ttaggcaatc ctatctcgca gcattagcaa aatctccaga
     4321 tgggaatgtt tcagttggaa atgtaacctt tgaagctggc tgtcgtaata attggcatgt
     4381 tcatttggac ggttatcaaa tcctcctcgt tacagaaggt tcaggttggt accaagaaga
     4441 gaataaaaag gcagtttcat tagaaccagg taacgtgatt gtaactgata aaggcgttaa
     4501 acattggcat ggtgctaaaa aagattctga atttgcacat attgcgatta cagctggtaa
     4561 gagtgagttt tatgaagctg ttagtcttga agaatattca agactaggtt aaattcaagt
     4621 aaggaggaat caatccatta tgaaagaaaa acaaacagcc ggacgtcgtc aattagaaga
     4681 atttgcacct gaatttgcca gatataatga tgatatttta tttggtgagg tttgggcaaa
     4741 agaggatcat ttaacagata aaacacgttc tattattact atatctgcct taattagctg
     4801 aggaaatctt gaacagttag agcatcatct tcaatttgcc aaacaaaacg gagttactaa
     4861 agaagaaatt gctgacatta tcactcacct cgctttttat gttggatggc caaaagcttg
     4921 gtcagccttt aataaagcta aggaaatttg gatataaaat aacagacata gcaagggaga
     4981 tactttccca aaagccaaaa agaaagatca accaattatt aaaccaacta ttaggagaat
     5041 gatatgctta ctattcaaga aatgaaagat acaacctttg aattacaaga aaattttaga
     5101 aggttaaatt atcctattaa acaagttgct aaagacttgc aacttaatat cagtgaagtt
     5161 gaatctcttt taagtcttga tgttacttat cctggaaatg tttgtatgct gagagattat
     5221 ctagaagata tgttgaaaaa agagggtaaa gaagtttatc cattttctag attagcatct
     5281 cattcagcaa atcgttggta cccttatgag acaccatggc gttattaaag actaaagatt
     5341 aatccatcat ctttagagaa ataaacaaaa atagtcaata gattcttttt agaaaaaagc
     5401 ta
//
LOCUS       DATROY010000121         5383 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.122, whole genome shotgun sequence.
ACCESSION   DATROY010000121 DATROY010000000
VERSION     DATROY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(12..1031)
                     /locus_tag="VBD11_001697"
     CDS             complement(12..1031)
                     /locus_tag="VBD11_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6954627.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            1135..2262
                     /locus_tag="VBD11_001698"
     CDS             1135..2262
                     /locus_tag="VBD11_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6954628.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            complement(2299..3285)
                     /locus_tag="VBD11_001699"
     CDS             complement(2299..3285)
                     /locus_tag="VBD11_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6954629.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            3448..3754
                     /locus_tag="VBD11_001700"
                     /pseudo
     CDS             3448..3754
                     /locus_tag="VBD11_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3912..5285)
                     /locus_tag="VBD11_001701"
     CDS             complement(3912..5285)
                     /locus_tag="VBD11_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954630.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
BASE COUNT         1892 a         1097 c          786 g         1608 t
ORIGIN      
        1 aaccttattt attattttct aaaaatcgct ttagatatga tataggtcat tataattgac
       61 aaaagaattg tggtagctaa tgctaacagt tgaactggca tatgtgataa tttaaagaga
      121 ccaactccta aacttcccaa caatccccca ataccattca ttccaaagga ggaaacgaca
      181 tcatcaatgg caaaagcttt cgcaacgtaa tccgttacaa agtaggtgaa gagggatgca
      241 aagaatgtta cagctaataa ttgccaagtt aagagatacc caactcctgc tgtactcgtc
      301 acaagaccaa caataatgcc attcaataag cttcctgttt tctcttcctc accattccac
      361 ttagcagcca atgtccacgc caaaccacca cctataatag caaatatagt atttaaaagt
      421 atcataatgg cttgtgaatt ccactcacca actggaccca tattaaatcc aaaccaacca
      481 aatgtaataa gtatcattcc taaaaagaga ctatcatttt taaccttatt gtgctgatgt
      541 tcactcttgc caataacatg agctaaaata tacgaagaaa gccctgcaga taaatgaacg
      601 accattccgc ctgaaaaatc aagtactccc atctttgcaa aaacaccctc tgaattccaa
      661 attaaataag ctaagggcgt atacaccaag aaaagccaag aaacaacagc taataaaagt
      721 cgcttcgtct gaactctatc aataattgac ccaataagca ttactactgc gtaaagggcg
      781 aaacaaagtt gaaataaaca atttacaatt tgtttaccat ctgcttcttg gaattgaaag
      841 acagttttaa aatgaccttc aaatgctaaa aaataggcac ccatgaacca agctgttgtc
      901 gttagcaaaa cagtaagtac aaactgataa ataatccttg aagtcaatga ttgatgtagc
      961 gacccaaagt aatagaatgc aaccccaaaa atcatgagcc acatcgataa aatacataat
     1021 agtaaaaaca caaatagtcc tttctttaca aacactcttt tatttgttaa actgtattgt
     1081 tgttaactgg ttaagtattt tatcttacac atttattttt gtcaaggaga agacatgaaa
     1141 cattatcaaa ccataattat cggtgcaggc gctgcaggaa tcggttttgg ttctgccatg
     1201 caacgattag gacttacaaa ttttcttatc attgaaaaag ggcacattgg agaatctttt
     1261 ttaagatggc ccagaacaac acaatttatc acaccttcct tcactaccaa tggctttggt
     1321 tttccagatc taaacgctgt tattccagat acttctcctg ctttctcatt tgaaaaagaa
     1381 catctttctg gagtggaata tgcacgctac ttacaattag ttgctgctca ttataatctc
     1441 cctatccaaa acgaaacatc tgttctatct attgataaac gagatagcct ttttgttatt
     1501 aaaacttcaa agggagattt ttctgctgat tatcttatta tggcaacagg agaatttcaa
     1561 aatccaaata ccattgatat taaaggtgca gatctaggaa tgcactatgg tcaagttgat
     1621 aatttccata tcaaatctga caatccattt atcatcattg gtggaaacga aagtgcttgt
     1681 gatgcactaa cacatctagt atatttggga aatcaagtcg aattatatac agatacgttt
     1741 ggtcgtaaag agagcaatcc cgatccaagc atctctttat cccccctaac taaagaacgt
     1801 ttaaaacata tacaagacca caaaaaagaa tattatagta tctctgaagg caagaaagcc
     1861 attgagatta aacaaattgg aaaacaatat caagttactt ttgatgacgg tagtactgcg
     1921 gagtctttcc ataaacctat tctttccaca ggttttctaa atacctgtca tctcattgac
     1981 ggaatcgctt tatttgaata cgataaaaat cagttgccaa tagttactga agatgacgaa
     2041 tctactatcg ttaataactg tttcttaatt ggtccaagct tacgacaaag agatactatt
     2101 ttctgctata tttataaatt cagacagcgc tttgtccctg ttattaatca tatcgcacaa
     2161 cgagaaggga ttatattaga cgaagatacc cttgaattct ttaaagaaaa tcaaatgtac
     2221 cttgataacc tagcttgttg cgatgtcaat tgtgattgct aaaaaatcgc tagctttatt
     2281 gctagcgatt ttttaatatt aaatatttgc tacttgaggt aaggattctc caatagcttc
     2341 taaacgttct gcccattctt gacgtgataa accattttct tgcacatagc gattacgttc
     2401 atgtgcacga cattcaggcg agcaccctcg aacatacttc gcttcgtttt cttctgaagc
     2461 aaaaatttgt ttgttacaaa acggatttgc acaattgaca taacgttcac aaggtgttcc
     2521 atcaaaataa tctttactaa taacagttgg attaacatga ttaattggca ccgagatacg
     2581 atcatcaaaa acatacatcg caccatccca aagttcacct tgaacttccg gatcttttcc
     2641 atatgttgca ataccaccgt gtaattgtcc aacatcttta aaaccttctc gaaccatcca
     2701 acccgagaat ttctcacaac gaacaccacc ggtacagtac acaacaacac gtttttccat
     2761 aaacttatct ttgttgtcac gtacccattg tggtaactca cggaaattgc gaatatcagg
     2821 acgaattgct ccacggaagt gacctaagtc atactcataa tcattacgcg tatctaaaac
     2881 aacagtatct tcatcaagca aggcttcttt aaattgttta ggatttaaat actcacctgt
     2941 tgtttccaaa ggattaatat cgctatcaaa gttattatct tctaacccaa ggtgcacaat
     3001 ctctttctta taacgaacaa acattttacg gaaagcttgt tgattttcct cgtcaatctt
     3061 gaaccaaaga tcagcaaaac gctcatcgct gtggatccaa tccatatact tctgagttgt
     3121 ttcataatct ccagaaacag ttccattaat cccctcatcc gcaatcaaaa tacgaccttt
     3181 aagcccaata gatttacaaa attctagatg tttcgcagcg tactcttccg cattttcaat
     3241 tgaaacatat ttataataga gtaaaactct gattttttct gacatttttt ctcctcatct
     3301 gcaaaataag ttagtatact tcattatata acgactcctt acaacatagc aaaaaatacg
     3361 cttaggacat attctttgct ttaaagacaa attattcttc ttaagaacac acatatgtta
     3421 aaatagtaaa agactaggag gacttttatg acttatgaat tatgtctaga atacggtact
     3481 tatccactta gacctgtaga tgcttgggct gatgaaatca atactgcacc tgcgtttatt
     3541 actgaagata aaaaaacttt tagaactttt agaagaagtc aataccttat tccatgagct
     3601 ctttttaacg atagaatgtt ccttccacta tattggtcat gacttcccag aaaaaagagc
     3661 aaaaatcaca caaatttatc atgtgattat cgaacattta agcatacact accctgaata
     3721 tgacattaaa attgaatcat tattaatggg gtagttttat tttaactagg tagaaactat
     3781 ctattaaaaa caatcgatat cactaattaa ttcaacaaat agattatcta aaacaaaaaa
     3841 ggtctctttc aagaggcctt ttttcataac ttatctcact aaatattaaa tgatactttt
     3901 accaaataat atcaccaata ctctagactc ttatctcttg aaagcaaggt acagacaata
     3961 gccgcaaatc cagaaatacc aaatattata aatgctactc cccaaccaaa gtggtcaata
     4021 ataataccaa tagctagagt agcgatcgtt gctcctccga cataactaaa tgtgccagtc
     4081 aatcctgaag ctgctccagc tgctgctttt ggaactaact ccatagcata caggccaacc
     4141 atcataacag gaccgtagat tgatgccccc aaacctagta aaataatgaa gtctaatgcc
     4201 atatattgag gtcctgctgg aataaaccaa taggcagcta gtaataagat tgctacaact
     4261 aagaaggcta cattggttaa aggtttagag tttttaaaca ctttggcaga tagatatcct
     4321 gctgttagca tcccaataat accacctatt tcaaaaagtg aaaaaccacc cataccagct
     4381 gttgcagtaa taccttttcc accatcttgc acactcgtcg ctaaaaactt aggagtccat
     4441 gagacaatac cgtaacgtaa aatatataca aatattgaat acaaggatgc ccacatcagt
     4501 ttagtatttg gtagaatata tttttttata atttcgccca tacccaaaac ttcaggctct
     4561 tctaaaatgt cacttgaatc cacaacatta gtatattgct ctttatgata ctcttctata
     4621 ggcggaagtc cgatagattc tggagtatct tcttgtaaaa agtaaacaag aacagccaaa
     4681 agacaagcca ccacccctgg aaaccaataa gcagcacgcg cttgattaag agaatcatta
     4741 ccagcaagag ctagacctac accagttaaa atcggcgcta ttgctccacc aaaattgtga
     4801 gataaattcc aaatggtagc atatttaatt ctttctttat tatcatacca atagactaat
     4861 gtacgcgctc caggagggta tcccatccct tgtaaccaac cattaataag atacatgata
     4921 gtaaagagcc agatactagt aattacagat ggaataacac cgattaacac attaactagt
     4981 cctgtaagaa acaaaccaac tggaaggaaa attttagaat tcgattcatc tgaaatcaaa
     5041 ccagaaataa atttagagag accataagca atgtagctag cacaggtaat aataccaatc
     5101 tcagtagcag acatattaag gataccagat aaaattgaag tcgtattacg aacaaaatag
     5161 tatcccatat aacctacaaa aatagaaata aggacttgta cacgataacg aggatattta
     5221 tgcttaattt cctcagctga taaacgttct cttttctgag ggggtgcaaa tagttttttc
     5281 aacatagcaa attacctttc cgatttttag atgtgtttaa ttatacaact atgaattcac
     5341 actgtcaatt aattaataat ttatttttta tcattttatc tta
//
LOCUS       DATROY010000122         5238 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.123, whole genome shotgun sequence.
ACCESSION   DATROY010000122 DATROY010000000
VERSION     DATROY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            19..147
                     /locus_tag="VBD11_001702"
     CDS             19..147
                     /locus_tag="VBD11_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6954631.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            208..1521
                     /gene="obgE"
                     /locus_tag="VBD11_001703"
     CDS             208..1521
                     /gene="obgE"
                     /locus_tag="VBD11_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6954632.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            1547..1705
                     /locus_tag="VBD11_001704"
     CDS             1547..1705
                     /locus_tag="VBD11_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954633.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
     gene            complement(1798..2148)
                     /locus_tag="VBD11_001705"
     CDS             complement(1798..2148)
                     /locus_tag="VBD11_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954634.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTT
                     IIAYLVIFILSISTVILNLVLLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTI
                     VKNALQYIKKLKNV"
     gene            complement(2298..3038)
                     /locus_tag="VBD11_001706"
     CDS             complement(2298..3038)
                     /locus_tag="VBD11_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6954635.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(3038..5221)
                     /locus_tag="VBD11_001707"
     CDS             complement(3038..5221)
                     /locus_tag="VBD11_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6954636.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
BASE COUNT         1633 a          873 c         1034 g         1698 t
ORIGIN      
        1 gtaaatgagg tgacatgagt ggcttttgga gataacggac aacgaaaaaa aactgggttt
       61 gaaaaactaa ctctttttgt tgttattttg atggtactag taacagttgg tggtttggta
      121 tttggtgcta ttagtgcaat catgtaatga gaaacgctgt aaagcgtttt tttatactga
      181 atagaataaa gaataggaat tttagaaatg agtatgtttt tagatactgc caaaattagt
      241 gttaaggcag gtcgcggtgg tgatggcatg gttgcttttc gccgtgagaa atatgtcccc
      301 aatggaggcc cgtggggcgg tgatggcggt aaaggcggat cggtcatttt taaagtaaac
      361 gaaggtttac gaactttgat ggattttcga tataaccgta attttaaagc aaaagctggt
      421 gaaaaaggga tgactaaagg tatgcacggt cgaggtgcag aagaccttat tgtgtcatta
      481 ccacctggaa caactgttcg tgatgctaat acaggtaagg ttattaccga ccttgtggag
      541 catgatcagg aattcgttgt agccagaggt ggccgcggtg gtcgtggaaa tattcgcttt
      601 gcaaccccac gtaatccagc tcctgaaatt gctgaaaatg gggagcctgg cgaagaacgt
      661 gaattacaat tagaattaaa aattttggct gatgttggtc tcgttggttt cccatcagtt
      721 gggaagtcga cgctacttag tgttgtttca gcagcaaagc ctaaaattgg tgcctatcat
      781 tttacgacaa ttgtccctaa tttagggatg gttcgtacaa aatcaggtga tagctttgca
      841 atggctgatc tccctggttt gattgaaggg gctagtcaag gtgtcggatt aggaactcag
      901 ttccttagac atattgagcg tacacgtgtt attctacatg ttattgatat gtcggcaagt
      961 gaaggacgag acccttacga tgactatatt tcaattaata atgagttgga aacttacaat
     1021 cttcgattga tggaacgtcc gcaaattatt gttgccaata aaatggatat gcctgattca
     1081 gaagagaatt tggcagcatt caaagaaaaa ttagctgcaa actatgatga gtttgatgac
     1141 atgcctatga tttttccaat ttcaagttta gcacatcaag gactagagaa ccttatggat
     1201 gcgacggcta aattattagc caatacagaa gagtttcttc tttatgacga aactgacatg
     1261 caagaagatg aagcatacta tggatttaac gaagatgaac gtccatttga gattacacgt
     1321 gatgatgatg caacgtgggt attgtatggt gataagctag aaaaactctt tgttatgaca
     1381 aatatggaac gcgatgaatc aataatgaaa tttgctcgtc agttacgtgg tatgggcgtt
     1441 gatgaagcac ttcgagagcg cggagctaag gatggagata ttgttcgtat aggtaacttc
     1501 gaatttgaat ttgtagatta atgcagtact tgattggagg ttttttatgg gagataaacc
     1561 gatatcattt cgtgataaag atgggaactt tgtttcagcg gccgatgtct ggaatgctga
     1621 aaaattagaa gaactcttta acacattaaa tcctaatcgt aaacttcgtt tagaacgcga
     1681 aaaattagca aaagaaaaag gatagttaag taactatcct ttttagttat tttgggtatg
     1741 aaaaaagact ctaaaacata attgatagag tgttaaggta cagattactt tattatttta
     1801 aacgtttttg agctttttga tatactgaag agcatttttt acaatggttg gagtataagt
     1861 aataaattta taagcaaaat aaaaagcaca aactaaagtt ataaaaaata gtaagtaaat
     1921 aatagggttt tgcgttaaga atccttgaaa aagaagtaca aggttaagaa ttacggtaga
     1981 gatacttaag ataaaaataa ctagataagc aataatagtt gtccaaaacc ttaaatggat
     2041 gttactcacc ttttcccatt taatatctaa aaagtcatct ataaagttga catgataagt
     2101 catatgttct ttaataaagt gcaatattct gttaatatag tctttcatac tgctatatta
     2161 tcatactgaa agcccttgtg caacattatt caaacattct agctgatgtt ttgctaattt
     2221 tagaagaaaa cttatcctat tataatttgt ttgtgatcag gatataaaaa aagatgacac
     2281 gaagtcatct ttttttatta aacgtttaaa actttattaa ggaagtcttg gagacgtggg
     2341 tgttgaggtg tatcaaagat ttgctcaggt gtcccatctt cgaggaaacg accaccatct
     2401 gtaaagataa cacgattggc aacttgacga gcaaatccca tttcgtgtgt cacaattaac
     2461 atagtcatac cttgttcagc taaatctttc ataacattga gaacatctcc aaccatttct
     2521 ggatcaagtg cagaagttgg ttcgtcaaaa agtaagatat ctggattcat cgctaagctt
     2581 cttgcgatag ctacacgttg tttttgtcct ccagataaac tatctggttt ggcattagct
     2641 ttatcggcta aaccaacttt ttcaagtaat tccatcccat gcttttcagc tgcttcttta
     2701 gattctttac caagttctat aggagcaaaa gtgatgtttt caagaactga catatgtgga
     2761 aaaagattga agtgttgaaa taccattccg atattttcac gtgctttgtc tatgtctgtt
     2821 tttggatttg ataactcaaa gccgtccaca acaacttttc cacttgtgat tgattcaagt
     2881 aagttcaatg tgcgaaggaa agttgatttt cctgaaccag aaggtccaat aatacaaaca
     2941 acatcacctt cataaaattt agcatcaata ccttttaaaa cttcattttg cccatacgac
     3001 ttgtgtaaat cttggacatc aatttttaat tctgccatta tttaagcctc ttttctaaac
     3061 gttttgctag acgggtgaga agggtaatga ttacaagata cataattgca aggatagcat
     3121 acatacggaa tgattgatag ttacgtgcaa ttatgatttt accagtttgg aagagttcca
     3181 caagtccgat tgctgataca attgttgtat cctttaatga gatgacaaat tggttgataa
     3241 agtttggtaa cataaggcgt actgcttgag gtaagataac cttttgcata gttttgccgt
     3301 aagaaatacc taagctgcga cttgcttcca tttgaccaga aggaacagct tcaataccac
     3361 cacgtacaat ttcagcaatg tacgcaccac catttaaaga aagagcgata gtagcagcaa
     3421 cgaagtcatt aattggactt tggtgacctg tgatgctttc gattaaatta ggaatacccc
     3481 agaaaataaa agcggccaca atcatgagtg gaataccacg gacaatatca acaaaaatca
     3541 ttgaaattgt gcggagagta ttacttggtg atacgctcat cataccaaag ataataccaa
     3601 taaccatagc aatcgcaaac gagataagag ttaaacttaa agtagttcca ataccagata
     3661 gcaattgttt gtagttatta gaaattaacc ctaaaatagt tgattcatct acaggtttag
     3721 cagctttatc gtttgaagaa gtgctggctg tggaaaggta ttttttaaca agtttatcgt
     3781 actcacccga ttttttgagt gaagcaagac cgttgttaaa cattttaatt aattctggat
     3841 ttgccccttt tttcactgca aatccgatat cgcctgattt ttcacctttg ataggtgttt
     3901 caaatttacg accttgatta atagcgtaag caagaacggc ttcgtcatcc attagagcat
     3961 caattgaacc tgagttcata ctatcataca ttgtagatgc ttcatcaaat gctttaacat
     4021 gatagttgta cttatctgcg tggtctgata accaagtata tgaggcagta ccatttttag
     4081 caccaactgt ttttcctttt aaatcttggt atgatttgac attgcttcct tttttaaccg
     4141 ctaagataac gctagatgtg taataaggat cagaaaaatc aaagattttt tggcgtgctt
     4201 ctgtgattgt ggctcctgca ataacaccgt cagcttgccc agattggaca gcatttaaag
     4261 cggcatcaaa acctggattt gagatatcaa gtttaaaacc ttgctgttta gcaattttcg
     4321 tgattaattc catatcaaaa ccagtatatt tccctttacc gttttgatat tcgaatggtg
     4381 cgaatgaaga atcagaaaca attttataac ttggctttac aggagttgct ttttcattgg
     4441 cattacctgt tgctttgccg cttgtttttg tagcgtcttt tccaagccat ttatccatga
     4501 tgtcattata agtgccatct gatttcattt gtgcaaaagc tgtgttaaat tctttaatta
     4561 gattatcgtg tccactacct tttttgacag caaatgcaaa gctaccaact gcttcgcctt
     4621 ccatgttaat ggcgtaagct tttccttgat ttatcgcaaa ttgcacaact ggttgatcgt
     4681 ccatagcggc gtaaatagaa ccagaatcaa ggctgttatt cattaggtcg cttgtatcaa
     4741 atgttttaac tttatagccg tatttagatt tattttcttc taagaagctt tgagcagctg
     4801 ttccattttt tacaccgact acttttcctt ttagttgttt gtagtttgtg actttattat
     4861 tatttttagt ataaagaata acggaagtat cgtaataagt atctgagaaa ttaaagactt
     4921 ttttacgtgc ttcagtaaca gtagttccgg ccattagcgc atctgcctgt ccagattgaa
     4981 cagcgttaac tgcggcatca aaacctggat acgtcatgtt aacattccag ccagcacgct
     5041 tagcgacttc gttaacgatg tcaacatcga ttcctttata agtttgatca gaatctttaa
     5101 attcgaatgg agcgtatgct gtatcagaaa caatattcac agtctctgca ctgacctttc
     5161 caccaaacac aaagaatatt gagagtaaag caagcgtaaa agcttttaac ttgtgcttca
     5221 tgtaaatctc ctttttat
//
LOCUS       DATROY010000123         5179 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.124, whole genome shotgun sequence.
ACCESSION   DATROY010000123 DATROY010000000
VERSION     DATROY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            75..3508
                     /gene="srr2"
                     /locus_tag="VBD11_001708"
                     /pseudo
     CDS             75..3508
                     /gene="srr2"
                     /locus_tag="VBD11_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
     gene            3618..4871
                     /gene="secY2"
                     /locus_tag="VBD11_001709"
     CDS             3618..4871
                     /gene="secY2"
                     /locus_tag="VBD11_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6954637.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            4881..>5179
                     /gene="asp1"
                     /locus_tag="VBD11_001710"
     CDS             4881..>5179
                     /gene="asp1"
                     /locus_tag="VBD11_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6954638.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWP"
BASE COUNT         1689 a          880 c          958 g         1652 t
ORIGIN      
        1 tttaaatatg gaggaaaaat gttaaaaagc agtttggaaa ctttggtgaa aaatcacgca
       61 aagttcgagt taaaatgagg aaatcaggta agcactgggt caaaagtgtt atgacacaaa
      121 ttggctatgt tatcctttcg aggtttagcg gtaaagagaa aagctctaag gttcaaacta
      181 ctagtgaaga tttaagcaga actaaaacgt ctgctagcat cctaacagca gtcgcagctc
      241 ttggagctgt agttggaggg acgacagaca caacttctgt ctcagctgaa gaaacaccta
      301 ctgcaacaga acttacagga aatgaaaaaa cattagctac tgctgaaact gtagtggttg
      361 cgccagaagt aaaaactgta aactcagatt cttcatcaca ttcaacaagt gaatcacaat
      421 caatgtcaac tagtaccttg caatctacca gcgcctcatt atctgccagt gaatcactaa
      481 tggattctac ctctgcttct ttatctgagt ctagctcgtt atcagaatat agttctttat
      541 ctctatctag tagtgagagt gtctcagcat cagaatcagt tcaatcatca gaagcggcaa
      601 cgaccgctag agttcaaccg agagcgatga gagttgtatc tagtgcttca gatatggaaa
      661 ctttaccagc agcattaatc tctggtgaag gagatgtaac aactgttcaa ggacaagatg
      721 taacagataa gttacaaaat ttagatataa agctctctgg aggagtgcaa gcaaaagcag
      781 gtgttataaa tatggataag tcagaaagta tgcacatgtc tttaaaattc actattgatt
      841 ctgtgaatag aggtgatact tttgaaatta agttatcaga taatatcgat acaaatggag
      901 cttctaacta ttctattgta gaacctataa aatcacctac gggtgaggtc tatgcgactg
      961 gaatttatga ttctcaaaaa aatctatagt ctatagtttt acagattttg cggcttcaaa
     1021 aaataatatt aatggaatat tagatattcc attatggcca gatgatacga cagttcaaaa
     1081 cacaaaagaa gatgttcttt tttcggtaaa aataaaggat caagaggcta caattaaaga
     1141 aacagtgaaa tatgatccac cggtaagaat tgattttgca gggggagtaa gtgtagattc
     1201 tcgaataacc aacattgatg atgtgggaaa aaagatgact tatataagtc aaattaatgt
     1261 agatggaaaa tcactctata attacaacgg gttatatact aggatatata attatagcaa
     1321 agagagtaca gcagatttaa agaattcaac gataaaaatc tataaaacca cctcggataa
     1381 tattgtagag agtatggtac aggattattc aagcatggag gatgtaacta gtaagtttgc
     1441 aaatagttac ccagaaaaag ggtggtatga tatttattgg gggcagttta ttgcatcaaa
     1501 tgaaacgtat gtaattgttg tagaaacacc atttactaat gcagtaactt tgaatactac
     1561 tttatcagat tataatgaga acaatggtgt agaacataat catacttact catctgaatc
     1621 gggatattca gatgtaaatg ctcaagaaag aaaaatttta tccgaattag taagtagctc
     1681 agaatcagta tcaagttcag aatcggtatc taattccgaa tctatttcaa cttctgaatc
     1741 ggtatctaac tccgagtcta tttcaagttc cgaatcggta tcaagctcag aatctatttc
     1801 aacttctgaa tcggtatcaa cttcagagtc tatttcaagc tccgagtcag tatcaagttc
     1861 agaatcggta tcaagctcag aatctatttc aagttccgaa tcggtatcta actccgagtc
     1921 tatttcaagc tccgaatcgg tatctaactc cgaatccatt tcaagttctg aatcggtatc
     1981 aagctcagaa tctatttcga attcagagtc tatttcaagc tccgagtcag tatcaacttc
     2041 agagtctatt tcaagctccg aatcggtatc taactccgaa tccatttcaa gttctgaatc
     2101 ggtatcaagc tcagaatcta tttcgaattc agagtctatt tcaagctccg agtcagtatc
     2161 aacttcagag tctatttcaa actccgagtc ggtatctagc tccgagtcag tatcaacttc
     2221 agagtctatt tcaagttccg aatcggtatc aaactccgaa tccatttcaa cttccgagtc
     2281 agtatctacc tcggaatcta tttcaagttc tgaatcggta tcaagctcag aatctatttc
     2341 aagttccgaa tcggtatcaa actccgagtc tatttcaaac tccgagtcgg tatctagctc
     2401 cgagtcagta tcaaattcag agtctatttc aagttccgaa tcggtatcta actccgaatc
     2461 catttcaact tccgagtcag tatctacctc ggaatctatt tcaagttctg aatcggtatc
     2521 aaattcagag tctatttcaa gttccgaatc ggtatcaaac tccgagtcta tttcaagttc
     2581 agaatcggta tcaaattcag agtctatttc aagttccgaa tcggtatcta attctgaatc
     2641 tatttcaagt tccgaatcgg tatcaaattc agagtctatt tcaagttccg aatcggtatc
     2701 aaactccgag tctatttcaa gttccgagtc agtatcaagc tcagaatcta tttcaagttc
     2761 cgaatcggta tctagctccg agtcagtatc aaattcagag tctatttcaa gttccgaatc
     2821 ggtatctaac tccgagtcta tttcaagttc agaatcggta tcaacttcag agtctatttc
     2881 aagttccgaa tcggtatcaa attcagagtc tatttcaagt tcagagtcag tatcaagttc
     2941 agagtcagta tcaagctcgg aatctatttc aacttcagaa tcggtatcta actccgagtg
     3001 tatttcaagt tccaaatcgg tatctaattc tgaatctatt tcaagttccg aatcggtatc
     3061 aaattcagag tctatttcaa gttccgaatc ggtatcaaac tccgagtcta tttcaagttc
     3121 cgagtcagta tcaagctcag aatctatttc aagttcagaa tcggtatcaa actccgagtc
     3181 tattttaagt tccgaatcag tatcaagctc agaatctatt tcaagttcag aatctatttc
     3241 aagttcagag tcagtatcaa tgagtactac agagtctcta agtgaatcag aagtatcagg
     3301 ggattctgaa attagctcaa gtacagaatc atcaagtcaa tctgaatcga tgaatcatac
     3361 tgaaattaaa tccgattccg aatctcaaca cgaagttaag catcaagtat taccagaaac
     3421 aggtgataac tcggcttcag cattaggtct gttaggtgca ggattgttgt taggagctac
     3481 taaatctcgc aagaagaaaa aagattaatt ttcaaagtga tatttagatg ataaatcgaa
     3541 agtactttcg atttatcact taagatagta agtagttata ctgtcttaag tgataaattg
     3601 atttgaaaag ggaaaaaatg attggaaaat tatattatag ctatagaaag tcaacgctat
     3661 taagaagtat tttatggact attttaattg ttggtgtata tatgttagga caacgtgttt
     3721 tattatccac tgttccttta tcacatcaag agataaaact agcagtagat caacatttac
     3781 tcaataactt ttcagcagta agtggtggga gttttaataa attaaatgtt ttcacactgg
     3841 ggttgagtcc atggatgtca agtatgatta tttggagatt cgtttcctta ttttcgtggg
     3901 caaaaaatgc aacgaagcga aaagcagaag tagctcaata tactttaatg cttactatct
     3961 cagttataca agcatatggt gtttcaggaa atcaatttat aaaaagctct ttattaggtt
     4021 cttatagtga tattgttttt aaagcattga caatggtctt gttaataagt ggctcatttg
     4081 tgttaatgtg gcttgctaat ctcaatgctt ctaaagggct aggaggaagt attgttatca
     4141 tattagtgag catgctccaa gcaacgacta ctggaataat aacttatttt gaacagtcac
     4201 attttagtat tatggatctg ctgggaacta ttatagctat aagtataata cttagtgttt
     4261 tattattaat ctcaacggtg gtttataggg cagaatatcg tatacctatt aggcgtattg
     4321 atatcgttag taaatttgcc caagatacct atattcctat tcgcataaat ccagcagggt
     4381 cctacccttt catgtatggg atgacattaa tgatgcttcc aacgtttata attcaagcat
     4441 tgttacttgc ttttccaaac gagcgattat tgattcagat ctcgtcaatg attgcaatta
     4501 ataagccttt aggtattgga ctttttgtta tattattatt tattttgagc attggatttg
     4561 catattttaa ttacgacccg tataatattg caaaaaatat gcgtcaacgg ggggaatatt
     4621 ttcaaaatgt aactccagga gctgctactc aacgtttttt acaagaaaaa attagtatca
     4681 tggcagtagt tggagcaatt tttacggtta ttatgggagg tctcccaatg ttggctgtgg
     4741 tggggaagac aaaccaagtt agtattgcaa tgatcgtcat taatatttat attatttcaa
     4801 cttttatgct cagtattatt gagcaagtta ataccttgaa gttgtggggc agtcaccgaa
     4861 atattattta aggagcaatc atgttttatt ttattccttc gtggtacaat tctgatcaaa
     4921 agtggcataa tgaagctcct atctggtatc gtgtattgga gaacatgact tttgatgata
     4981 ctgttagtca ggttagaatg tttcagaatg cagatgagga agtgcgttta cttctattta
     5041 gttataatcc tcagttgaga tacatgcttc atgaagagag tatgttggct gtctcttatt
     5101 ggtctttttt tgacgatatt caaaatattc aacaaaaaga gattaagtta attaatttta
     5161 aagaactgaa ttggcctaa
//
LOCUS       DATROY010000124         5057 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.125, whole genome shotgun sequence.
ACCESSION   DATROY010000124 DATROY010000000
VERSION     DATROY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..625)
                     /locus_tag="VBD11_001711"
     CDS             complement(<1..625)
                     /locus_tag="VBD11_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6954639.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYA"
     gene            complement(639..1268)
                     /locus_tag="VBD11_001712"
     CDS             complement(639..1268)
                     /locus_tag="VBD11_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6954640.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(1278..1919)
                     /locus_tag="VBD11_001713"
     CDS             complement(1278..1919)
                     /locus_tag="VBD11_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6954641.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(2054..2383)
                     /locus_tag="VBD11_001714"
     CDS             complement(2054..2383)
                     /locus_tag="VBD11_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6954642.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(2546..4360)
                     /gene="glmS"
                     /locus_tag="VBD11_001715"
     CDS             complement(2546..4360)
                     /gene="glmS"
                     /locus_tag="VBD11_001715"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6954643.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            complement(4629..>5057)
                     /gene="lepB"
                     /locus_tag="VBD11_001716"
     CDS             complement(4629..>5057)
                     /gene="lepB"
                     /locus_tag="VBD11_001716"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6954644.1"
                     /translation="NGDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMD
                     DILYLNDVFKDEPYIEKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLN
                     DNRQNKNDSRKFGLIKEKDIRGVITFKVYPLSEFGFTASE"
BASE COUNT         1651 a          988 c          724 g         1694 t
ORIGIN      
        1 tagcataaac ttcatcaatc aaaagaccat caatacggtt attttttaaa tcaatcaaag
       61 cctgagtgaa agtatcgtat tgaactgctt cttttccttt tacaaacttt tttaaaatat
      121 caggtttagc gttaaaagca tcaaaaccag atgaacccga ctgggctcct agttttttcc
      181 ccttcatatc cttaatacta ttaatatgtg atgaagtttt agtaacaatt acttgatgat
      241 tattcatata tgggtttgta aaagcgactt ttttagcacg ttctgccgtt tttgaataac
      301 cattccaaat aaggtctata ttaccattat taagttcagt ttctttcata tcccagttaa
      361 taggctgcca tttcactgaa ataccgtatt ctttaaaaac agcattagct aaatcaatat
      421 caaagccggt atagtcacca gaacgacttt caaatcccat aggaacaaaa gtattatcaa
      481 atccaatagt aattttcttt tccttttgat aatgttccca attatcaata ccagccattt
      541 ccttattaga cataccacat gctgataata taatcataaa taatccaaat ataatggtta
      601 ataatatatt tttgtgagtc aacaatctac ccccaagtct acttaggatt aattttgaga
      661 atcgtatcag atatactttc agcaaattga agatcatgtg ttactacaat ttgtgtcata
      721 cctgtttctc tattttgtaa aattagtttt tctacttctt gacgcaactc tggatcaaga
      781 gcgctagttg gctcatcata accaataatc tgtggatcaa tcatcatcgc acgagctagt
      841 gcgacacgtt gcttttgccc cccagataat gaaaatggat agactaaagc atggtcttta
      901 agtcccaagc gttcaagtaa tactaaggct ttttctttag caagttcttt tgataatccc
      961 atcgttttaa caggagagag gatcaaatta tctaatactg ttaaatgtgg gaataattga
     1021 aagtcttgga agacgaatcc taaaagattt agtgtttcca aatgatcaac agatacttct
     1081 ttaccatcgt gaacaatagt ccctgaatca atcttttcca atccagctaa catacgtaat
     1141 agtgttgttt ttccacctcc agaaggtcct actaaagaga gaattttacc ttcttctaca
     1201 gtcagattga aatctttaaa gatttctttt tgcccatagc atttagaaat attttttaat
     1261 tctaacatcg tccctcctta tttatagtag ttaaaacgct tttcaacttg tttagataaa
     1321 atagttaaaa gaccaattaa taataggtaa ataccacctg ctataaacat tggtgcaagt
     1381 gttgcatccc tatttgcggc tgtcttactt tctagaagta agtcgcccac accaagtatg
     1441 taaaccaatg aggaatcttt cactagagta atcgtttcat tgaaaacact cggtaagact
     1501 attttgaaga cttgaggtaa tactatgtac caaaccgttt gaaacttaga aaactttaat
     1561 acctttgcag cttcatattg ccctttagga atagactcaa tacctcctcg aaaaatctct
     1621 gcaaaataag ctgcataatt catcgtaaaa gcaataatcg cagctggcat acgatcaaaa
     1681 gaaattccta cacttggtaa aaagtaataa aaaaagataa gctgtaataa cagaggcgtg
     1741 cctcgcataa tccaaatata agttgtcagt agccattgaa gcggcttaaa atgaactttc
     1801 attaggaagg ctacaacaat accaagtgga attgataaac aaataacaat aaagaaaact
     1861 tgcaatgtca cttgcgcacc tactaataag ctaggtaaga tttctaaaat ataagacatg
     1921 gtccccccta aataaaaaat agatgttctc attatatcaa aattcaaacc aatgtcatag
     1981 agctttttta ataaaaatgc atgaaatcaa gtcatattca aaaagcaatt gaccgtcgcc
     2041 aattgcctga aagctatttt tttataaact cagattttag tttcatagct ccaaaaccat
     2101 caattttaca atcgatatta tgatcgccat caactaaacg aatattttta acacgagtgc
     2161 cttgcttaat atccttaggc gctcctttta cttttaaatc cttaataact gtcacagtat
     2221 ctccatctga taagcgcgtt ccattgctat ccaatacaat taaacctact tcttcttcga
     2281 tttcttctgg attccactcg taagcacatt ctggacaaac cagtaaaata ccgtcttcat
     2341 agacatattc agagttacac ttagggcaat ttggtaatga catttttctc tccttaaatt
     2401 aattaataaa atagtcagca agtaacattc taactgatta taggcttctt gacaatagga
     2461 aaaagcctat tttccttcaa aaataggctt ttttacaatt tttaatattt tgtagttaat
     2521 attagcgtat tttagtgtaa cgagtctatt caacagttac agcttttgcc aaattacgtg
     2581 gtttatcaac atctaagcca cgttgtaatg aagcatagta agcaattaat tgagtcggaa
     2641 taaccattga aatggctgat aagaatgggt gaactttatt aacaataata tcatcacctt
     2701 cacgttccaa cccttcttct acaataatca aagcatttgc tccgcgagaa acaacttctt
     2761 ggatatttcc acgagtatgc gctgcaatag ttgaatcagc agatattaaa gcaataacag
     2821 gagtattgtc ttcaatcaac gaaattgtac catgttttaa ttcacctgca gcaaaacctt
     2881 ctgtctgaat ataagaaatc tcttttagct tcagtgcagc ctccattgta acataatagt
     2941 cgttaccacg accaatgtag aaggcattac gtgtcgagat taacaactgc tctaccttct
     3001 cagagataac atctttttct gataaggtcg cttcaattga ttgtgctaca attgacaatt
     3061 catgaaccaa atcaaaatct tttgcttctg ccttaccatt tgcttctcct accgcttttg
     3121 ctaagaaagc taacgttgca acttgagcag tatatgcttt tgtagaagca acagcgattt
     3181 caggtcctgc atgaatgagc atcgtatagg ttgcttcacg agaaagtgtt gagccaggca
     3241 catttgttat agttaaacta gggattccca tttcgtttgc tttaactaaa acttgtctac
     3301 tatctgctgt ttctcccgat tgactaagta aaataaacat tggcttctta ctcaaaagtg
     3361 gcatgttgta accccattct gaagaaactc caagttcaac aggtgtatca gttaattttt
     3421 cgatcatggt cttagcagca aaaccagcat gatatgacgt tcctgcagcc aatatgtaaa
     3481 gacgatctgc ttcttggact gattttataa tatcagaatc cacattcata tctccagatt
     3541 cgttcgcata agttgaaatc aacttacgca tcacagttgg ttgctcatca atttctttaa
     3601 gcatgtagaa tggataagtc cccttaccaa tatctgataa gtcaagctct gctgtgtagc
     3661 taccacgttc aatgacatta ccatcataat cttgaacttc aacgctatct tttttaacaa
     3721 taacgagttc tttatcgtgg atttccatat attcacttgt ctcacgtatc atagccatgg
     3781 catctgaaca caccatatta taaccatttc ccaaacctat tagaagaggt gatttatttt
     3841 tagctacgta aatcgtatca gcatcttgag aatcaataag agcaaatgcg tatgatcctt
     3901 ctataatatg taaggctttt ttaaaggctt caagcactga taagttatct tcttcaacaa
     3961 agtgttcaac taaatgaata gctatttctg tatcagtttc acccttgagg ttatgttttg
     4021 ttaagtaagt ttctttaatt tgaaggtaat tttcaataac accattgtgt accaatacaa
     4081 aacgaccaga tcctgaagtg tgaggatgtg catttccttc agtaggttta ccatgggttg
     4141 cccaacgtgt atgaccgata cctgtcgtac cactaacaga atctcctact tttgcctgaa
     4201 gctccgcgat tcgaccgact gacttcacaa gttgactttt gttatcacct actacaaaaa
     4261 tacctgctga gtcatatcct cggtactcta gcttttctaa gccttgaatt aaaatatccg
     4321 ttgcatttgt atttccaact acacctacga taccacacat attattatta ccaataagag
     4381 ctttctctta tactttctat ctaaactttc tttttgaatt ggtatatatt aatatatacc
     4441 gttaaaaagt aacaaaaaca gtatataatt aatgtttttg tttgtcaata aaaaaattgg
     4501 tatagtcttt ttgattttct gtgataacga gataaacgct atcaaaaatt ctatctatct
     4561 taaaaaaata tatttcttag ccattttatt acataaaaag aatggtatca aataatacca
     4621 ttcttttact actctgatgc tgtaaaacca aattcactta gagggtatac tttaaaggta
     4681 ataacaccac gaatatcttt ctctttgatt aatccaaatt ttcgactatc atttttattt
     4741 tgtcgattat catttaacac aagataagaa cccttaggaa ctctgctttc tttatttcgt
     4801 gttaatgttt caactgagaa gtcggaagta aagggcattt gaccgtcttt cttttcagaa
     4861 taagcatttt tcattttttc aatgtaaggc tcatccttga aaacatcatt caaatataaa
     4921 atatcatcca ttactctcac tttttgattt ggctcaccaa taacacgact aatatagaaa
     4981 atctttccaa ccttatagac aataaagtct ttgtacttag gcgtcctatt acgattaaca
     5041 acaacaacat ctccgtt
//
LOCUS       DATROY010000125         5020 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.126, whole genome shotgun sequence.
ACCESSION   DATROY010000125 DATROY010000000
VERSION     DATROY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(181..1089)
                     /locus_tag="VBD11_001717"
     CDS             complement(181..1089)
                     /locus_tag="VBD11_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6954645.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            complement(1299..4999)
                     /locus_tag="VBD11_001718"
                     /pseudo
     CDS             complement(1299..4999)
                     /locus_tag="VBD11_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         1502 a          983 c          748 g         1787 t
ORIGIN      
        1 aaaaaggagt agcgattgct actccttttc caaaacccga aattaatgcg agccaaggtt
       61 ctaccttgtt ctttaatatt attatacact aatttcgtta agtgtcaaca actttttgaa
      121 aaataaatat tttctatatt tttaactaca actcaacttg atttccttca accaaaaaaa
      181 ttatgctttt ggtttaccgt aacctgttaa aagtaggata gtcatagcaa ctgtttcttt
      241 caatgatttc cgacgaagtt ttaattttga taaaagataa atccttctta ttagattggc
      301 attttttaat tctataaaac gactaaagaa gagtttacgc tccagggaca ttttatcgga
      361 tacttgagca aaaataagcg aagcacgatc aaaagaagta tttatcatct gccaaaacgt
      421 agccacccca tattgtctcc cgtaattatt tagcgactct gcccctactt gtctacgcca
      481 aaggactgta ctgtcagaaa tataagccac atttccaacg gcgtaagcca gagttccaac
      541 ataagaatca tgggcgccaa tttgacggta ttcccaaagc tttgctaact catgattcat
      601 catgataacc ataccattta tttggttaaa ggcaatttgt tcttgaataa ctgttgctgg
      661 attatgttct ttcaatactg ttaatttttc atctacagaa gtccagttcg aataaacaag
      721 caatggtaat tcttggttaa atttctctgc ttcttcaagg taacgttcta aacgattttc
      781 tttccaaaca tcatcctggt ctgagaaaac ataaaaatct gcttttttat agttaaccaa
      841 ctcgtaaaaa ctgcggtgac ttcctaactt tctatcatcg ttagaattaa taaatataaa
      901 acgtgaatct ctattaacat attctttaat aatagctact gtatcatcag tagatccatc
      961 gtcccttatt aaacaaatcc agtcagtata accttgttgt attatgctat ctaattgctg
     1021 gcgaaggtat ttttgaccat tataggtcgc taagcaaata tttatttgcc aatcttttat
     1081 cgttttcatt gctatccaaa ctccttaagg ctagttcatc ttatgaaagc ttacaaagcg
     1141 aacagtatca catactaatc gtaattataa tattataata ttatatgccg ttaatcaata
     1201 taaaattaat caaaaataag agtcaatacc ctaaaagtcc agcttaacat aatgttaaac
     1261 tgaacttttc atcatattac cgagtagttt acttagtttt atttactatt atttttagtc
     1321 tttttgggtt tcaagtaaag aagtaaacta gataacaatg ccaaagtgcc aacaaataga
     1381 ccattcgttg ctctatctga tgtactaggt aaggtatggt taacagaatc cccgttatgt
     1441 ttaggtgata tgatcttagc tactctgcta ctattattgt ttgtagaagt gaggactttc
     1501 tcatttcctg atttagttat ttctttagac aatgattgag tagtaactgt agtttgaggc
     1561 ttaactaatg ttatttcttc tagattcttg tttactactt ttccttcttt agaaataaac
     1621 ataggttctt tcttcaagtt atcaaaatat ttgaaactgt tagaaattac tatagcgttt
     1681 tctttctctg atactactgc cttattcaag aggtcagaca attttaccgt tgcaaaatta
     1741 ccagctttat cttccacaac aagtgtcaag gccttagggt ctaccgcaac ctcactctct
     1801 cctatcttaa ccgttttagg aagtgtcact ttaccttctt gatctatatg gaaataatgg
     1861 taagaagtct catccccata ttcttcatct tttacaacat gagataaaac taattgtaaa
     1921 cgatatgtag gaacataact actttcctta ggcatggcta agcttaatgt tcgattagtt
     1981 tcatcaaact gagctcgtga aggaagattt ggtgacttag tacttacttg aactttaaag
     2041 tctgactcct gactatttgc tccttctgct actggtgtgt aacgtaagcg ataagtataa
     2101 aaaccatctg ctacaacttt gccatcctta tctaaaccac tccactgaag agcatccata
     2161 cgataatgac catcactttg ctttggatta ttatggaaat ttttacgata agatggtaaa
     2221 accttacttt gccaaataac atttccattt tgatctagaa cttgagcaga aatatcctta
     2281 acatttctta agaaagttgc ctggggagtg atttcgtccc tatttccatc tttatttgga
     2341 gaaatggcaa aatatggatt attcgctgca tctctttcca aaagatgaat tgttttatcc
     2401 tcaaccttat tctcaaaagt tcctaaaata attctttttg gactctccgg tgctaattct
     2461 aacttcccac catttttgac ataatcaaca tagccccaag acgctgattg tgttaacaag
     2521 gcagtatagt tgttgctttc aaaaggagct gattcattgt actccaattg gtctttatga
     2581 gttgtatcat ttggtttata gtagaaacta cctttagaaa gcgtcttata aatcggtgtt
     2641 tcaagtgctt gtaagttcgc aaaatcacca ttaaatccta caaaaggaat actcattaac
     2701 tcctgattac tatccttggc ttctttaaaa cgtacaaaac cttctaagaa ataaccattt
     2761 gccatctgtt cttttaattt ctgactaaat tgactagcat caatagtaaa tcgaacttgt
     2821 gtttctttat cacgaagaat tactttctgc caattagtat ctagcaaggc ttgtggttta
     2881 agggcaaatt tacctttatt tacttgttct gttgctacat tagcttgata atacaattct
     2941 ttgacacctt ctacaagttt atgaattgta actgtgatat caaatttatc tccctctcgt
     3001 ttgagattaa ttttaacttt gccatcgttt ccagtaacat aatattgagc ttggatagct
     3061 ttttcagcat caactacacc tgcaccttgc tgacgtggtg aataaaacgc cttatcctct
     3121 tcactatata atgctgttgc tgagctcatg aggatgtttt tagacaattc tagcaatttt
     3181 ttagaatcta aattcatccc tttatatttc tcagccaaat gactttgaag cattgtcatt
     3241 aatcctgcaa catgtggtga agccatactt gtaccagaca ttgtttagta ttgattatta
     3301 taggttgaag aataaatttc aaagccagaa gctgttacat caggcttgat tgctccttca
     3361 gctgtcacgc cccaacttga ttgttccagc atacgattgc caccttggct atcaactact
     3421 tcaaaactct ggttaaatgt taactgactt gaagtatttt ttatacgctc gccatctact
     3481 ttactaataa cccccacagg taattcacgg taaggaatta gaaaatttcc acgtttttct
     3541 tgatcgttaa aaataacgat accaacaaca cctgcatttg tagcatgagt gattttagtc
     3601 ataaaatcaa gtccaccacc acgctcaatt aatgcaatct tacctttaaa gtccttacct
     3661 tcaaaatctt ttttgcacca taattggcat aaaccacatc gtaggcctta cctttgtcaa
     3721 aaggtttaga agtcacaatc ggcaacttaa ctaacttacc ttcaatagtt gtttcaacga
     3781 cctcactgat agttttaagt gattcatagc tagcaacact caaagtatct tcagaaatag
     3841 ctggactatt aaccgtaccg tagtcaggat tagttgataa tggtttgcta taatccatac
     3901 caaatgcacc ttcatttccg gcagccacaa caactgcaac gcccttctca gaagctaatt
     3961 taagtgctaa tttaacttta tcattgagag caattaaaga atcagccgtt tttcccaggc
     4021 tcatattaat cgtttttgct cctagattaa cagcgtctat gattgcttta gcatatgctt
     4081 ctccaaattt gtccgaatca attttatctg gaatacgcat taataagact tgagcatttg
     4141 gcgctgcacc ttctaaaaga agactattga ttgctggacg tttactatta cctacaaaaa
     4201 taccagcaac gtgtgtacca tgcaaaatat tctttgcttc tgacccataa ccatctttca
     4261 tagctgctgc aatatcagcc accgtttctg tattgttggc gtagttatgt gcaaaaacaa
     4321 tcttatcgtt aacccatttc ccataagtga tattatgttt tgcttttaat tcctcaaatt
     4381 ctgccttagt tttaaagctg tgcttatcat cttttgggct atctaaacga aaaatatcat
     4441 ggttaatatc aaagccagta tcaataatag ctactacatt tccctttcct ctttgggttg
     4501 tggcttgtaa ttttgttatt ttagatgtat caagaacagc aagcgatgac ttgctttctg
     4561 acttcacctc ttcatatgct gagggaactt tctgtgctat tgcagttgaa gcatttgtta
     4621 ctacattgct ttctttattg ttggttgtct ctggtttaga gggatattct tcttcaagat
     4681 cagcccccaa attagacgta tcaaggtttt tagataactc ttctaataat tcatcttctg
     4741 ttttgtcatt ttttacagat gtatcaccca tttctttcac tgtattacta gcagaagcag
     4801 ctgttacaga tgttttttca acaatattag tagttaccga tggcgatggc tgttgagcaa
     4861 cattagcaat tacaggtgat tgctcttggt tttttaattc ttgctcctct gcattcactt
     4921 gattgctatg cattaataaa atactagttg ttataagtgt taactttaaa atagcctttt
     4981 ttgagtgatg tttatccact tttcctattt cctccaaaaa
//
LOCUS       DATROY010000126         4999 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.127, whole genome shotgun sequence.
ACCESSION   DATROY010000126 DATROY010000000
VERSION     DATROY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            98..1646
                     /locus_tag="VBD11_001719"
     rRNA            98..1646
                     /locus_tag="VBD11_001719"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VBD11_001720"
     tRNA            1701..1773
                     /locus_tag="VBD11_001720"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            1928..4830
                     /locus_tag="VBD11_001721"
     rRNA            1928..4830
                     /locus_tag="VBD11_001721"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4905..>4999
                     /gene="rrf"
                     /locus_tag="VBD11_001722"
     rRNA            4905..>4999
                     /gene="rrf"
                     /locus_tag="VBD11_001722"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1410 a         1015 c         1445 g         1129 t
ORIGIN      
        1 gacgaacgaa tgtgcagggt ggatttacat tgtaaatccg tcaatgacaa aaaacaataa
       61 atctgtcagc gagacagaaa agagcaaagc tcaaactttt aatgagagtt tgatcctggc
      121 tcaggacgaa cgctggcggc gtgcctaata catgcaagta gaacgctgag gtttggtgtt
      181 tacactagac tgatgagttg cgaacgggtg agtaacgcgt aggtaacctg cctcatagcg
      241 ggggataact attggaaacg atagctaata ccgcataaga gtaattaaca catgttagtt
      301 atttaaaagg agcaattgct tcactgtgag atggacctgc gttgtattag ctagttggtg
      361 aggtaaaggc tcaccaaggc gacgatacat agccgacctg agagggtgat cggccacact
      421 gggactgaga cacggcccag actcctacgg gaggcagcag tagggaatct tcggcaatgg
      481 acggaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct
      541 gttgttagag aagaacgttg gtaggagtgg aaaatctacc aagtgacggt aactaaccag
      601 aaagggacgg ctaactacgt gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc
      661 ggatttattg ggcgtaaagc gagcgcaggc ggttctttaa gtctgaagtt aaaggcagtg
      721 gcttaaccat tgtacgcttt ggaaactgga ggacttgagt gcagaagggg agagtggaat
      781 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accggtggcg aaagcggctc
      841 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc
      901 tggtagtcca cgccgtaaac gatgagtgct aggtgttagg ccctttccgg ggcttagtgc
      961 cgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag
     1021 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag
     1081 aaccttacca ggtcttgaca tccttctgac cggcctagag ataggctttc tcttcggagc
     1141 agaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
     1201 ccgcaacgag cgcaacccct attgttagtt gccatcatta agttgggcac tctagcgaga
     1261 ctgccggtaa taaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac
     1321 ctgggctaca cacgtgctac aatggttggt acaacgagtc gcaagccggt gacggcaagc
     1381 taatctctta aagccaatct cagttcggat tgtaggctgc aactcgccta catgaagtcg
     1441 gaatcgctag taatcgcgga tcagcacgcc gcggtgaata cgttcccggg ccttgtacac
     1501 accgcccgtc acaccacgag agtttgtaac acccgaagtc ggtgaggtaa ccttttagga
     1561 gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt agccgtatcg
     1621 gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa acctgccatt tgcgtcttgt
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccattgaatc gaaaggttca aattgttcat
     1801 tgaaaattga atatctatat caaattccac gatctagaaa tagattgtag aaagtaacaa
     1861 gaaaataaac cgaaaacgct gtgaatattt aatgagtttt ctagttttaa agaaactagt
     1921 ttaataaggt taagttaata agggcgcacg gtggatgcct tggcactaga agccgaagaa
     1981 ggacgtgact aacgacgaaa tgctttgggg agctgtaagt aagcgctgat ccagagatgt
     2041 ccgaatgggg gaacccacta gttaatgact agtatccata tctgttaagg atatgagaag
     2101 gaagacgcag tgaactgaaa catctaagta gctgcaggaa gagaaagcaa aagcgattgc
     2161 cttagtagcg gcgagcgaaa cggcaggagg gcaaaccgaa gtgtttacac ttcggggttg
     2221 taggactgcg atataggatt aatcattata gaagaattac ctgggaaggt aagccaaaga
     2281 gagtaacagc ctcgtattta aaatgataga ttaaccttag cagtatcctg agtacggcga
     2341 gacacgcgaa atctcgtcgg aatctgggag gaccatctcc caaccctaaa tactctctag
     2401 tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga
     2461 aatagaacct gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg
     2521 ccttttgtag aatgaaccgg cgagttacga tatgatgcga ggttaagttg aagagacgga
     2581 gccgtaggga aaccgagtct taatagggcg ccatagtatc atgttgtaga cccgaaacca
     2641 tgtgacctac ccatgagcag gttgaaggtg aggtaaaact cactggagga ccgaaccagg
     2701 gcacgttgaa aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga
     2761 gatagctggt tctctccgaa atagctttag ggctagcgtc gatgttaagt ctcttggagg
     2821 tagagcactg tttgggtgag gggtccatcc cggattacca atctcagata aactccgaat
     2881 gccaacgaga tataatcggc agtcagactg cgagtgctaa gatccgtagt cgaaagggaa
     2941 acagcccaga ccaccagcta aggtcccaaa atatatgtta agtggaaaag gatgtggggt
     3001 tgcacagaca actaggatgt tagcttagaa gcagctattc attcaaagag tgcgtaatag
     3061 ctcactagtc gagtgaccct gcgccgaaaa tgtaccgggg ctaaaacata ttaccgaagc
     3121 tgtggatgac acaaaagtgt catggtagga gagcgttcta tgtgtgaaga aggtgtaccg
     3181 tgaggagcgc tggaacgcat agaagtgaga atgccggtat gagtagcgca agacaggtga
     3241 gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt
     3301 cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat attcctgtac
     3361 tagagtatat agtgatggag ggacgcagta ggctaactaa accagacgat tggaagtgtc
     3421 tggtcaaaca gtgaggtgtg atatgagtca aatgcttata tctttaacat tgagctgtga
     3481 cgaggagcga agtttagtag cgaagttagt gatgtcacac tgccaagaaa agcttctagc
     3541 gatacatata ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag
     3601 gtgagcgaga gaactctcgt taaggaactc ggcaaaatgg ccccgtaact tcgggagaag
     3661 gggcgctgat tttagatcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac
     3721 agctctctgc taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg
     3781 ttaagaggag tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg
     3841 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa
     3901 aggcgtaatg atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga
     3961 agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata
     4021 ttgagtatct gtaccacatg tacaggatag gtaggagcct atgataacgg gacgccagtt
     4081 tcgtttgagg cgttgttggg atactaccct tgtgttatgg ctactctaac ccagataggc
     4141 aatccctatc ggagacagtg tctgacgggc agtttgactg gggcggtcgc ctcctaaaag
     4201 gtaacggagg cgcccaaagg ttccctcaga ctggttggaa atcagtcgta gagtgtaaag
     4261 gtataaggga gcttgactgc gagagctaca actcgagcag ggacgaaagt cgggcttagt
     4321 gatccggtgg ttccgtatgg aagggccatc gctcaacgga taaaagctac cctggggata
     4381 acaggcttat ctcccccaag agttcacatc gacggggagg tttggcacct cgatgtcggc
     4441 tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg
     4501 cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag
     4561 gaaatttgag aggatctgct cctagtacga gaggaccaga gtggacttac cgctggtgta
     4621 ccagttgtct tgccaaaggc atcgctgggt agctatgtag ggaagggata agcgctgaaa
     4681 gcatctaagt gcgaagccca cctcaagatg agatttccca tgattttata tcagtaagag
     4741 ccctgagaga tgatcaggta gataggttag gagtgtaatt gtggtgacac atttagcgga
     4801 ctaatactaa tagctcgagg acttatccaa aaagagatat tgacaacgtt acggtttctt
     4861 gttagactat agatattcaa ttttgagtga ataagactca atagttaagt gataattgcc
     4921 taggagatac acctgttctc atgccgaaca cagaagttaa gccctagaac gccggaagta
     4981 gttgggggtt gccccctgt
//
LOCUS       DATROY010000127         4906 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.128, whole genome shotgun sequence.
ACCESSION   DATROY010000127 DATROY010000000
VERSION     DATROY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            14..84
                     /locus_tag="VBD11_001723"
     tRNA            14..84
                     /locus_tag="VBD11_001723"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:tcc)
     gene            119..192
                     /locus_tag="VBD11_001724"
     tRNA            119..192
                     /locus_tag="VBD11_001724"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Ile,seq:gat)
     gene            206..293
                     /locus_tag="VBD11_001725"
     tRNA            206..293
                     /locus_tag="VBD11_001725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Ser,seq:gct)
     gene            529..1836
                     /locus_tag="VBD11_001726"
     CDS             529..1836
                     /locus_tag="VBD11_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6954646.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVVSSTPRAVSKPVVRSYDSSNTYPMGQ
                     CTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGYGHVAVV
                     TSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1960..2928
                     /locus_tag="VBD11_001727"
     CDS             1960..2928
                     /locus_tag="VBD11_001727"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6954647.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            3036..4211
                     /locus_tag="VBD11_001728"
     CDS             3036..4211
                     /locus_tag="VBD11_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6954648.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEASTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            4201..>4906
                     /gene="recO"
                     /locus_tag="VBD11_001729"
     CDS             4201..>4906
                     /gene="recO"
                     /locus_tag="VBD11_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6954649.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFYDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLK"
BASE COUNT         1540 a          790 c          984 g         1592 t
ORIGIN      
        1 atataagttt ttagcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
       61 cgagttcgat tctcgtcacc cgcttacttt acataaaagt aaaaccaagc atttgcttgg
      121 gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc
      181 cactcgtgcc cattaatatt aatttggaga attactcaag aggctgaaga ggacggtttg
      241 ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct ccgtaaatat
      301 tataatttta agagtaatta tactcttttt ttatttctct tttagtttca ctatggagta
      361 attatattga tagagtgaat agaagaaggt tatcgttttc tagatttgat tatggttcat
      421 ttagaaatag ttgatttcaa atataaatgt aacaatgacg taatataatt tcgtgaattt
      481 tttggtaaaa tatttaatgt cttatcgtaa aggagtagtt taataaaaat gaaaaaaaga
      541 atattatcag cagtccttgt gagtggagta actcttggaa ctgctgccgt aacagtaaac
      601 gctgatgact ttgactcgaa aattgctgct actgattcag taatcaatac tttatcaggg
      661 caacaagcag cagcacaaaa ccaggtgact gctattaaag gacaagttgg tgctttagag
      721 tctcaacaat cagaattaga agcgcaaaat gctcaacttg aggcagtatc tcagcagtta
      781 ggtcaagaga ttcaaactct ttctaataaa attgttgcac gtaacgaatc tttgaaaaag
      841 caagttcgta gtgctcaaaa gggtaacttg actaactata ttaatactat tttaaattcg
      901 aaatcagtat ctgatgctgt taatcgtgtt gttgctattc gtgaggttgt ttcagctaat
      961 gaaaagatgt tagcacaaca agaggctgac aaagcagcct tagaagctaa acaaattgaa
     1021 aatcaaaatg ctataaatac tgtagcagct aacaagcaag ctatcgaaaa taataaagca
     1081 gccttagcaa cacaaagagc acaattagaa gcagctcaat tagagttatc tgctcaattg
     1141 actacagtcc aaaatgaaaa agcatcttta attcaggcaa aagcacaagc tgaggaagct
     1201 gcgcgtaagg cagctgaagc acaagcagta gctgaagcga aagcacaagc tgagtctgta
     1261 gcaaaagcac aggcagcagc acaagttgag tctgccacag ctcctactga aacagttcaa
     1321 acacaaccga gaactgaaat aaagccttct aatcttacgg ctacttcttc ggcaactact
     1381 gttgctaaaa ctacagctac agctactaat gagccaaaag ttactcaacc ttctgtagta
     1441 actaaagcag ttgtttcttc aacacctaga gcggtatcaa aacctgttgt cagaagttat
     1501 gattcaagta acacataccc aatgggacaa tgtacttggg gagctaagtc aatggcttct
     1561 tgggttggta actattgggg aaatgctaat cagtggggtg ctagtgcacg tgcagctggt
     1621 tattctgtag gaactacccc tcgtgttggt gcagtagctg tttggccata tgatggtggt
     1681 ggttacgggc acgttgcagt agtaacgtca gtggctaaca attcatctat ccaagttatg
     1741 gaatcaaact atgctggaaa tatgagcatt ggcaattacc gtggttcatt taaccctagt
     1801 gcttctggct ctgtttacta tatttaccca aattaatgaa tactaaggag atctttcgtg
     1861 gtctcctttt tgtttattaa ttaggtatgg tttattttgg attgaaaaat tgtgtaaaaa
     1921 gcgttaaaat ggtataggac taaagttttg gaggaaatca tgtcttattc taatttaaaa
     1981 ttgtttgcac tgtcatcaaa caaagaacta gcgaaaaaag tgtctcagac gattggaatt
     2041 ccgctaggtc aatcgacagt tcgccaattt tctgatggtg agattcaagt taatattgaa
     2101 gagtctattc gtggccatca tgtttttatt ttacaatcaa caagctcacc agttaatgat
     2161 aatttgatgg aaattttaat tatggttgat gctttaaagc gtgctagtgc ggagtctgtt
     2221 agtgtggtaa tgccttacta tgggtatgct cgtcaagatc gcaaggcacg ttctcgtgaa
     2281 cctattactt cgaaactcgt tgcgaacatg ctagaagttg ctggtgttga tcgtctttta
     2341 acagttgatt tacatgcagc tcaaattcag ggattctttg atattcccgt tgatcacctt
     2401 atgggggctc cccttattgc agattacttt gatcgtcagg gattagttgg tgatgatgtt
     2461 gttgtcgtta gtcctgatca tggtggcgta acacgtgcac gtaagctggc acagtgttta
     2521 aagacaccga ttgctatcat tgataagcgc cgtagtgtta ctaaaatgaa tacatctgaa
     2581 gtaatgaata ttattggtaa tatcaaaggt aaaaaatgta ttttaattga tgatatgatt
     2641 gatactgcag gtactatttg ccatgctgca gacgctttag ctgaagcagg ggcaacagct
     2701 gtttatgctt catgtactca ccctgtttta tcagggccgg ctttggataa tattcaaaat
     2761 tcagctattg aaaaattaat tgtgttagat acgatttacc tccctgaaga acgtttgatt
     2821 gataagattg aacaaatttc aattgcggaa ttgattggtg aagctattat ccgtattcac
     2881 gagaaacgtc ctttatcacc attatttgaa atgaataaac taaaatgata aaaaacctgt
     2941 tattaacagg ttttttatca tttaatgacg gaaaactgga cacaaattat ttttcttgta
     3001 taattaaata tattatttct tatcaggagg ttatgatgac attagagaaa cgatttaata
     3061 agtacttaga tagaatagag gtctccttaa ttcgtcagtt tgatcaatct atttcagata
     3121 tacctggaat ggtaaaatta actcttggag aaccagattt taccactcct gatcatgtta
     3181 aagaagcagc taaatcagct attgatgcga atcaatcgta ttatactggt atgagcggtt
     3241 tattagctct aaggcaagct gctgcagatt ttgcaaagga taaatataat cttacttata
     3301 accctgatag tgagatcttg gttacgatag gtgccacaga agctctgtct gcatctctta
     3361 tagctatttt agaagctggt gatgttgttc tattacctgc ccccgcttat ccaggctatg
     3421 agccaatagt taaccttgta ggagctgata tcgtagaaat tgatactaga gaaaatgact
     3481 ttagactaac ccctgaaatg ttagaaacgg ctattatcca acaaggtgaa aaattaaagg
     3541 ctgttttact taattatcct actaatccta caggtattac ctattcaagg caagaaattg
     3601 ctgcattagc tgaagtcctt aaaaaatatg atatttttgt tattagtgat gaagtttact
     3661 ctgagttaac ttacactggt cagcaacatg tttcaattgc agaatattta cctaaccaaa
     3721 ctatcttaat taatggctta tcaaaatcac atgctatgac tggttggaga gttggattgg
     3781 tgtatgctcc tgaagcatct actgctcaaa tcattaaaag tcatcaatac atggtgacag
     3841 ctgctagtac tatttcgcaa tttgctgggg tagaagcctt atctgtgggt aaaaatgata
     3901 cgttacctat gcgtcaagga tatattaaac gtagagatta tatcattgat aaaatgtcta
     3961 aacttggttt caaaataatt aaaccgagcg gcgcttttta tatctttgct aaaatacctg
     4021 atagttatcc acaggattct ttcaagtttt gtcaggattt tgcttatcaa caagctgtag
     4081 ctattattcc aggggtggct tttggtaaat atggtgaagg gtatatacgt ctttcttatg
     4141 ctgctagtat ggaagttatt gaaacagcaa tggctagatt gaaagttttt atggaatcat
     4201 atgagggtta gtcaaacata cggtctcgtt ttgtataatc gtaattatcg tgaagacgat
     4261 aaattagtta aaatctttac ggagactgag gggaaaagaa tgttttttgt aaagcatgct
     4321 tcaaagtcaa aattcaatgc tgtgctccaa cctttgacta ttgctcattt tattttaaaa
     4381 attaatgata atggtctttc ttatattgat gattataaag aagttttagc atttcaagaa
     4441 accaattcag acttgtttaa gttgtcatat gcgagttata ttacttcttt ggctgatgtg
     4501 gctattagtg ataatgtagc ggatgctcaa ttatttattt tcctaaagaa aacgttagaa
     4561 ttgattgagg acggtttaga ttatgagatt ctaacgaata tttttgaagt acaactacta
     4621 gagaggttcg gtgttgcttt aaatttttat gattgtgttt tttgtcatag ggcaggatta
     4681 ccttttgatt tttcacacaa atattctgga ttattatgtc caaaccatta ttataaagac
     4741 gagagaagaa accacctaga tcctaatatg ctgtacttaa tcaatcgttt tcagtcaatt
     4801 caatttgatg atttacaaac aatttctgtg aaacctgaga tgaaacttaa aattcgtcaa
     4861 tttttggaca tgatttacga tgaatatgta gggattcatc ttaaaa
//
LOCUS       DATROY010000128         4860 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.129, whole genome shotgun sequence.
ACCESSION   DATROY010000128 DATROY010000000
VERSION     DATROY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..123
                     /locus_tag="VBD11_001730"
     CDS             <1..123
                     /locus_tag="VBD11_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6954650.1"
                     /translation="AKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(182..1126)
                     /locus_tag="VBD11_001731"
     CDS             complement(182..1126)
                     /locus_tag="VBD11_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6954651.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(1137..1946)
                     /locus_tag="VBD11_001732"
     CDS             complement(1137..1946)
                     /locus_tag="VBD11_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6954652.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(2072..4309)
                     /locus_tag="VBD11_001733"
     CDS             complement(2072..4309)
                     /locus_tag="VBD11_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6954653.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            complement(4293..>4860)
                     /locus_tag="VBD11_001734"
     CDS             complement(4293..>4860)
                     /locus_tag="VBD11_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6954654.1"
                     /translation="GLNHFGTHHKEDDLNINLEKKVSTITEKKVPMISHVKDKVSNQV
                     TVDVKGAVNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQK
                     GEKITVVEEEKANNIATQGNSKGKINLNKADLSSLQTISGVGAKRAQDILDYRDSQGG
                     FKTIDDLKNVSGIGEKTLEKLRQDVTLD"
BASE COUNT         1631 a          901 c          735 g         1593 t
ORIGIN      
        1 gcaaaagaaa acattaaaca ccactatatc gttggtcaaa aaatgaacca cgtttatcca
       61 gtcttaccga ttcctgaagc agagactgct ttcaaacaaa tgcttaaaat tatcaatcat
      121 taaataaaaa ggtttccaat caagtttagt tggaaacctt ttttctatta cttttgaatg
      181 actaattatc ttgagataaa aactctgaga ataaaacttc ttgactaatc ttttcagcta
      241 aagcctgttt aaaagctaat ttccctaaat aataggattt gtggtcaatt gttgggagtt
      301 tcaaaagttg cccaactaat aaacattcct gaccaataat ataaggacat tttttcctac
      361 tttcttcata accttttatt agccctgccg caacttcatc acttgtagct aatataccat
      421 ctatactaac ttcttttacc aattgataag ataaatttag tccatcatta aagtcatgaa
      481 cattcccaac taccatatat ggagaggata gttgcccata gacctcttga taagctaata
      541 atgcggattg ataggtagca gaggattcat tattccttga aaataataaa actaagtgct
      601 ccaaacctcg taacttcata tcagaaaatg cctctagaaa tgaagaatag cgatcaaggt
      661 aagcagagga tagatgatta tattcctgca atttttcaca aactactatt cgtccatatt
      721 ttgcataagt ttcaataatg tcgaggctaa ttgctcttga agtgaatatt aaagcatcaa
      781 tagcttccat cttgagctgt ttcaagtagg aaagttctaa ttcttggttg taatccgaag
      841 gcatcacaac aagttgatag tcggtagttt tagcagcatc taataggcca tttattaatt
      901 gtgtaaaata aggatggcgc gtgtggggaa tgacgacacc tattttttgt gttttgcctc
      961 gacttaaatc ccgcgctagt tgatttggta tataatccaa ttcggtgata agagccatta
     1021 ctttttgtcg cgtctcatca gaaacatgtg ggtgatgatt gataacacgg gaaaccgttg
     1081 atttagcaac acctgccttt ttagctatat cactaatact tgtcattata aggctcctac
     1141 tttaaaaatt tttgaatccc atatcccact ccgtcttcat cattagactt agttagttga
     1201 tatgctatcg ctttgatatc atcaaaagca ttatccataa caatagggta acctaccatt
     1261 tcaagcatag gcaaatcatt atgtccatct ccaaatgcag ccgtttcttc tctagctaac
     1321 tgctcttttt gaaggatgta agcaattcct ttagattttt tggctaagag atgtgtaatt
     1381 tcaagatacg ctttacctga acgttgaata gtgatttctg gtaactccaa actttgaaga
     1441 tatttttcta attctagcat atttgcttca tcgaaagtaa tcatcataat tttaaaagta
     1501 tttgtgtgac cttctaaaaa ctgatcctca ttatgaataa acgttgggca ttgttgagtt
     1561 aaactatgtt cgtatcggat tccttcatct attttatcac aataccagtt attaagatcg
     1621 taataagata aactaacttg aggaaaatgg aaccgtattc ctcttagcaa ttgttttact
     1681 gtgctttttt taatgatttg tgtatgaata ggtagaactt gattattatc tcctattcta
     1741 taaatcaatc ctccattaaa agcaacttgg actcctccta attgaagtgc atctacggca
     1801 tccttcattt ccataggtgc cctagcagac actaaagtta ctggaatcgc tgcctcacgt
     1861 attaaggtag cgttactttt actcacttgt ccttcactat ttaatagtgt accatccatg
     1921 tcacaaaaaa tacgtttgat agtcataaaa aatacctctc ttcttctgaa atcagtataa
     1981 atgctggaac ccgttccatg tcaagcttaa aattactgaa tccttgcgaa aaatagattg
     2041 tatttaacag atacgcaatc attttaataa atcaacgaac tgtctgaata tgccacttcc
     2101 accatcctgt tagcctaatt gtacctgatt gatccgttcg gaaaatcttg cttttaatct
     2161 tttggaacct ttgtaaagtc tcttgatgag gatgctggta acgattattt ttaccagctg
     2221 acactagaac cacactagga gacaactttt tcagaaagga tagacttgat gagcccttag
     2281 aaccatgatg tcctgcttta aggatatcta ctgataaatt aggataagct tctaataact
     2341 gcttttctcc ctcttcctcc aaatcaccgg taaataagaa gccttttcct aataaatgtc
     2401 catataaaac aattgaatca ttattttttc catcaccctt gtgccatggg taaagtactt
     2461 gtaaataact tcccatgaca gaaagtttat caccagcttc tatagatttt acagctactc
     2521 ctatctttga taaacgctta acgtactgcg aattcgttaa agaacctgaa cttgtaatca
     2581 aacgtgcaac tttaaaatgc tttgagataa cttccatatc ccccatatga tctgtatcgg
     2641 tatgagttat cactaaatta tctatcttgc taatacctct gcttttcaag taaggaatga
     2701 gcgttctctc agcattagac tggtttactt tttgtttcca ttcttcaggc tgttcaaatc
     2761 tcacccttcc cccagtatca attaaaattg tttttcctaa ccaatccctc actaaaatac
     2821 tatctccttg tccaacatct aaaatcgcaa cctcgttagt aattggattc ttagtgataa
     2881 aaaacaaggt aaagataata agaagggagc aataacgaaa acatttagaa tgataataat
     2941 cataaagtaa tcccaaaatt ataatcatga ctattaaaag aaaaaggctg ggctttccaa
     3001 aaataagtgg tcttggaaac agttgcccag tccattttaa taatacttct aggatttcaa
     3061 acaaactatt aatacaactt aatttaacga acggtgataa gacaaatata acagttaata
     3121 aaggcaaaaa tataatatca aatgcaaaag ataaaagtgc tgttaatata attgatattg
     3181 gttgaaaacc cgaaaaatag taggttagta tgggaagaat tcctacagag actgtcaaag
     3241 attcgatagc tacttttttt atactcgaaa aatgatcaaa ggaggtcatc gtaagtataa
     3301 aagcataagc aaagctaaga actcctcccg tagtcaaaag tgagtggggg agagagataa
     3361 gacaaattaa caagcagaga gcaaagttct catctttctt gacaccgcta tgtgataata
     3421 aagattgaat aagtgcccta acgactgaga tactaaagcc tgttaaacca gcataaatta
     3481 gcgagaatgg tatttgaagt aaccaaacat ggtctagacg taagccaata cgtagacaga
     3541 tataacgaaa aataccgaga aaaaaaccta cttgcatacc tgacaaagca aataaatgta
     3601 taatacctag actactatat aaatcggaca tgtcatcaaa ggtcttatct agatatccaa
     3661 acaaaagccc tgacatatag tgacgcatag gatttggaaa agtttgacta ataacaattg
     3721 cacttctacg ccacttaaac ataagatcaa agaaagacct atgttgaaat aagcgtatct
     3781 gttttatctt tgtcactttg gcgatacgat aaattccatg tctttttaaa tactgacgat
     3841 aatcaaagcc attaaaatgc cttctctcct ctgcttcttc aagtttgata tctgcaatta
     3901 tcattatatt attatcaaga ttttgaaaat aatctttctc acttttattt tttaatgtgt
     3961 aaaaagcttg ataagtgtta cctgaagcat ctgcctggaa tgataaaaca tcaccattga
     4021 tagaaatagt atcaatacgc actttaatct ggctaatatg attcggtact ctttgatatt
     4081 ggacattcat attgtgattt tgccataaaa aataaaataa gaaaaaaatt actattgtta
     4141 ttgttttaaa aatagtttca cgatcatatt gataaatcag aaaaataaaa gtaaataaaa
     4201 gaaaggcaca accaagcatt gtccaagaaa acactagtaa gtaaatttgg aagaccaaca
     4261 atgctaaata aataggtttt agaggaaaat acttagtcaa gtgtaacatc ttgacgtaat
     4321 ttttctagtg ttttctcacc aatacctgat acatttttga ggtcatcaat agttttaaaa
     4381 ccaccttgac tatcacgata atctaaaata tcttgagctc ttttagctcc aacgcctgat
     4441 atggtctgaa gacttgataa atctgcttta ttaagattaa ttttcccctt actatttccc
     4501 tgagtagcaa tattatttgc tttctcctct tccacaacag ttattttttc acctttttga
     4561 gcaacataga taaccgtctc atcttgtaat ttctgtgcta gattaacgga tttactatca
     4621 gcgagattgc ttagtccacc tgcacgctta atcgcatctg ttacacgact ttgagaaggc
     4681 aaactataaa cacctggatg attgactgcc ccttttacgt caaccgttac ttgattagat
     4741 actttatctt tgacgtgact aatcattggt accttttttt ctgttatagt tgatactttt
     4801 ttctctaaat tgatatttaa atcgtcttct ttatgatgag tgccaaaatg atttaaaccc
//
LOCUS       DATROY010000129         4565 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.130, whole genome shotgun sequence.
ACCESSION   DATROY010000129 DATROY010000000
VERSION     DATROY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(65..940)
                     /gene="tehB"
                     /locus_tag="VBD11_001735"
     CDS             complement(65..940)
                     /gene="tehB"
                     /locus_tag="VBD11_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6954655.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            1083..3080
                     /gene="metG"
                     /locus_tag="VBD11_001736"
     CDS             1083..3080
                     /gene="metG"
                     /locus_tag="VBD11_001736"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6954656.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3276..>4565
                     /gene="brnQ"
                     /locus_tag="VBD11_001737"
     CDS             3276..>4565
                     /gene="brnQ"
                     /locus_tag="VBD11_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6954657.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLS"
BASE COUNT         1371 a          728 c          924 g         1542 t
ORIGIN      
        1 gtatgggttt agaatatact tattacctaa ccaaagataa aagtgagtag atttctactc
       61 acttttatga tcttaatttt cgagctaata aagtcgcaaa ctgtaatttt aagcgattac
      121 cgttttcatc caccctgtgc aattcaccta aattttcatt atatttgatg atttcccagt
      181 cattgtaata agatttcaat tgtccttctt tgaaggtaaa agggaatgga agcgggcaag
      241 gattttcagc ggtatccata gcagaaacga taagattgta tcctccaatc tgagtatgtg
      301 attgcatttg taaaataata tcagaaatgc agtcgggatt taaaaacata aataccacag
      361 ttgataaaat aaaatcatag tgcccttcaa tagcagcagt attaatatca taccttttta
      421 tattgtaagg aagctcttct tctaatgcca tattttctaa agctacaagg ctctgtccgt
      481 ttgaatcgac agaagtcact tgatgtccca gcagcgataa ataaagtgag tttcgccctt
      541 gaccacaccc aaggtctaat atattactct tctcagataa gagaggtgca ctggcgataa
      601 cctcagaatg tgtgcgcgtg agaccatatt ttttatggaa gtaatcttct ttttggcagt
      661 aaaaagttag atagcaactt aagtctggac tattcggcga aactttatgc cagacttgtg
      721 gatcaacaaa aggaatatca ctactagcat caaaaatatg ccgcgagatt tcctctccat
      781 caggagataa ctggtaaaat actaaagaac cacttagaat ggttagtttt gcccaggtgc
      841 cttccttagt attatgcttt tctaaaaatg cctttggaat agactgtgca gtccaaaccg
      901 gcatagtttt gtaggggaga agtgtttttg tattaaccat atattaactc tctattttta
      961 atacctttat tatagcaaaa gtagatatat tattcagaga tatatccagt tgaaaaagat
     1021 tttatgttat aatgtatcat gataaaaaac acctctcatt agggtggctt ataggagaaa
     1081 tcatgacaga aaaaaaatca ttttacatta ctaccccaat ttattaccca tcaggtaaat
     1141 tgcacatcgg ttctgcctat acaacgatag catgtgatgt tttggcacgt tacaaacgca
     1201 tgatgggctt tgatgttcag tatttgactg gtcttgatga gcatggtcag aaaattcaac
     1261 aaaaggctga agaagcaggc attacaccac aggaatatgt tgatggcatg gctgaaagtg
     1321 tcaaaacatt atgggaactt ttagatattt cctatgacaa attcatccgt acaacagata
     1381 cttaccatga agaagcggtt gctaaaattt ttgaacaatt gttggcacaa ggggacatct
     1441 atcttgatga gtataccgga tggtattctg tttcagatga agaatttttt actgaaagcc
     1501 aattagcaga agtttatcgt gacgaaaacg gtaatatgat tggtggtgtt gcgccatctg
     1561 gtcatgaagt tgaaaaagtt tctgaagaat cttatttctt ccgcatgagc aaatatgcag
     1621 atcgtttaaa agcgtactac gctgagcatc cagaatttat tcagccagat ggtcgtatga
     1681 atgaaatgct taaaaacttt atagaaccag gtttagaaga tttagcagtg tctcgtacaa
     1741 cttacacatg gggagttcag gtaccatcaa atccaaaaca tgttatttat gtctggattg
     1801 atgctttgat gaactatatt tctgctcttg gttatggttg gtctgacgat ctcagtcaat
     1861 atcataaatt ttggccagca gacattcata tgattggtaa agatattctt cgtttccact
     1921 ctatttattg gcctatcatg ttgatggcgc tggatttgcc attaccaaaa cgtctagttg
     1981 ctcatggttg gtttgtgatg caagatggta aaatgtctaa atcaaaaggg aatgtcgttt
     2041 atccagaaat gttagtagag cgttttggtc ttgatccact ccgttattat ctcatgcgtt
     2101 cattgcctgt tggttcagac ggaactttta cgccagaaga ttatgttgga cgtattaact
     2161 atgaacttgc taatgattta ggaaaccttc tcaaccgcac tattgctatg gttaacaagt
     2221 atttcgatgg agaagtacca aggtttgctg ttgcaactga ctttgatgcc gatttagctt
     2281 ctgttgcgac agatagtatt gaaaactatc ataaacaaat ggaagcggtg gatttcccac
     2341 gtgcccttga agctgtatgg aatcttattt cacgaactaa caaatatatt gatgaaacag
     2401 caccgtgggt acttgcaaaa gacgaaacag atcgtgataa gttagcagct gtaatgagtc
     2461 atttagttgc tagtctacgt gttgttgctc acctcattca accgttcatg atggaaactt
     2521 ccgatgccat tatggaacaa cttggtttag gggcaacatt cgatcttgaa aaattgactt
     2581 ttgctgatct accagaaggt gttcgagttg ttgcaaaagg ttcaccaatt ttcccaaggt
     2641 tggatatgga agacgaaatt acttatatca aagaacaaat gaatgctgga aaagcaccag
     2701 tggaaaagga gtgggttcct gaagaagttg aattgacatc aagcaaaggt caaatcaagt
     2761 ttgaggattt tgacgctgtt gaaatccgcg ttgcagaagt aattgaagtt gagaaagtgg
     2821 aaggttctga taaacttctt cgtttccgtc tagatgctgg tgatgagggc catcgtcaaa
     2881 tcttatcagg gattgcaaaa ttttacccaa atgaacaaga gttagtaggg aaaaaactcc
     2941 aaattgttgc taacctcaaa ccacgtaaaa tgatgaaaaa atatgtcagt caaggcatga
     3001 ttctctcagc agaacatgat ggtaaattga cagtgttgac ggtcgattca gcagtggcaa
     3061 atggatcaat tattggttaa tcgaaaagag cattatgctc tttttttgtt ttcaagaaaa
     3121 aagtgactga atagttggaa aattatgaaa atgacctaga atttattttt atttttgata
     3181 taataaaaga catcgattct aagcatttag atagaatttg ataaggagaa tttatggaaa
     3241 gggtgtaaat agattattta cactaatggt tgcttatggt aaaaaaaggc tttttaacgg
     3301 gattactctt atttggtatc ttctttggtg cagggaacct catttttcca ccagcattag
     3361 gtgtagcatc tggacaagat ttctggccag cgattttagg attttgtttg tcaggagttg
     3421 gattagctat tattaccctc ctactaggca cactaactaa tggtggttat aagacagaga
     3481 tgtcggaaaa atttagtccg tggttttctt taacattttt agttatactc tacctaacta
     3541 ttggtccact ttttgctatt cctagaacag caacggtttc ttttgaaatt ggggttgctc
     3601 caattgtagg gcatagtccg attgctcttt tgtgttttac agcctgtttt tttgctgcag
     3661 cttattatct cgctattaga ccaaatggta tattagatag tgttggaaaa atcttaacgc
     3721 ctgtttttgc ctttttaatt ctctctttgg ttgttgttgg agctattgct tatggaaact
     3781 tggagagtgc taaggcgagt gctgactatg caggaaaagc gtttggaagt ggtgtcttag
     3841 caggctataa tacacttgat gcacttgcag cagttgcttt ttgtctagta gcaacagaaa
     3901 cgcttaaaaa gtttggtttt aaaactaaaa aagaatattt atcaaccatt tggattgttg
     3961 gaattgttac tagtttagca tttagtattt tatatattgg cttaggtttt ttagggaata
     4021 agtttcctgt cccagctgat attttagctg acccaaatgt taataaagga gcttatgtat
     4081 tatcacaagc atcttataaa ttatttggaa attttggtcg ttatttcttg agtattatgg
     4141 taacattgac atgctttaca acaacagttg ggcttattgt gtcagtatct gaattttttg
     4201 ataagaactt tcgttttggg aattataaat tatttgctac tgtttttact ttgattggct
     4261 ttttaattgc taatcttggt ttaaatgctg taattacttt ttcagttcct gtgctaactt
     4321 tattgtatcc gattgttatt gttattgtct taattatttt gattaataag tggttgccgc
     4381 tttcaaaaaa gggaatgtct ttgacaattg gtcttgtcac tttagtgtcg tttgttgagg
     4441 ttctagcggg acaatggcaa gaaaaaacat taactcagtt agttggattc ctcccctttc
     4501 atactatttc aatggggtgg ctagttccaa tgttgattgg tatagtattt tctttggtgc
     4561 tgtca
//
LOCUS       DATROY010000130         4522 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.131, whole genome shotgun sequence.
ACCESSION   DATROY010000130 DATROY010000000
VERSION     DATROY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..367
                     /locus_tag="VBD11_001738"
     CDS             <1..367
                     /locus_tag="VBD11_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954658.1"
                     /translation="KFKKKVLNNFKYIKPAHLRNLSNRGIGIMESQHRKITYRMKRRG
                     MYWSKWGISTMANMIILERANGLRELFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKF
                     SKPLLKNGRKWSITGIKTK"
     gene            1021..4461
                     /locus_tag="VBD11_001739"
     CDS             1021..4461
                     /locus_tag="VBD11_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6954659.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGNAPSEMKEPYRLEGAMPEA
                     QLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETKKAFD
                     YAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPDKQLT
                     ETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIERGDI
                     DFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNPPKTI
                     TFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKLSGTS
                     MSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFSPRQQ
                     GAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYYQVTV
                     QTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKNGYFL
                     EGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDAKDQL
                     DGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTFAKQD
                     DDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNVVWTS
                     EVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISSGAKE
                     QHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDEDLPTT
                     EYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEGHSNK
                     PEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSSRTLE
                     KRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQKETKK
                     "
BASE COUNT         1594 a          889 c          886 g         1153 t
ORIGIN      
        1 gaagtttaag aaaaaagtat taaataattt caaatatata aaaccagctc atcttagaaa
       61 tctttcaaat cgtggtattg gtatcatgga atcacaacac agaaagataa cgtatagaat
      121 gaagcgacgt ggcatgtatt ggtcaaagtg gggaatctcc acaatggcaa atatgattat
      181 acttgaaaga gctaacggtt tacgagaatt atttttcggt tcttggagaa aggtatacag
      241 tgagtataaa gaaggttcat ttagtgcagg gcgacttttt aaaaagacag atgaattaga
      301 taaattttct aagccccttc taaaaaatgg cagaaaatgg agtataacag gaatcaaaac
      361 aaaatagaaa aagtctaaag atacctttat ttctagcgtt tttttgcatg ttttaacttg
      421 cattaaatct tctaaagtag tataattata ttaaagaaaa gcaaaaaagg tgaaggagcg
      481 cgaactccta caccaaaccg attaagacaa ataccacttg cttaatcagt cgaaccttat
      541 tgtagcataa ctaaaacaat accgtcaaga ctgaacaagc aaaaaatact tgttttggtc
      601 tttttaagaa taattcaagt tttcttaaat ttatgaggta tcgactaatc tcaaaatttc
      661 agacacatac ctgagtgaac atcaagagag aaccgatcgg ttctctcttg atgtctaaaa
      721 gaatgaggtt aaggaggtca caaactaaac aactcttaaa aagatgacct ttactaataa
      781 tcgtctttgt tttataataa agatgttagt aatataattg ataaatgaga tacatttaat
      841 cattatggca aaagcaagaa aaatagctgt gtcatatgca aataacccct gtttgctctt
      901 taaaaaagac gttatcctta tttctctaca cacagacgga tagctaggag agaatcgttt
      961 gattctctct tttcttaatg gtcataaaga caaagtctct catcatgaaa ggacgacaca
     1021 ttgcgtaaaa aacaaaaact accatttgat aaacttgcca ttgcgcttat atctacgagc
     1081 atcttgctca atgcacaatc agacattaaa gcaaatactg tgacagaaga cactcctgct
     1141 accgaacaag ccgtagaacc cccacaacca atagcagttt ctgaggaatc acgatcatca
     1201 aaggaaacta aaacctcaca aactcctagt gatgtaggag aaacagtagc agatgacgct
     1261 aatgatctag cccctcaagc tcctgctaaa actgctgata caccagcaac ctcaaaagcg
     1321 actattaggg atttgaacga cccttctcat gtcaaaaccc tgcaggaaaa agcaggcaag
     1381 ggagctggga ccgttgttgc agtgattgat gctggttttg ataaaaatca tgaagcgtgg
     1441 cgcttaacag acaaaactaa agcacgttac caatcaaaag aaaatcttga aaaagctaaa
     1501 aaagagcacg gtattaccta tggcgagtgg gtcaatgata aggttgctta ttaccacgac
     1561 tatagtaaag atggtaaaaa cgctgttgat caagaacacg gcacacacgt gtcaggaaat
     1621 gctccatctg aaatgaaaga accttaccgc ctagaaggtg cgatgcctga ggctcaattg
     1681 cttttgatgc gtgtcgaaat tgtaaatgga ctagcagact atgctcgtaa ctacgctcaa
     1741 gctatcagag atgctgtcaa cttgggagct aaggtgatta atatgagctt tggtaatgct
     1801 gcactagctt acgccaacct tccagacgaa accaaaaaag cctttgacta tgccaaatca
     1861 aaaggtgtta gcattgtgac ctcagctggt aatgatagta gctttggggg caagccccgt
     1921 ctacctctag cagatcatcc tgattatggg gtggttggga cacctgcagc ggcagattca
     1981 acattgacag ttgcttctta cagcccagat aaacagctca ctgaaactgc tacggtcaaa
     2041 acagacgatc atcaagataa agaaatgcct gttatttcaa caaaccgttt tgagccaaac
     2101 aaggcttacg actatgctta tgctaatcgt ggtacgaaag aggatgattt taaggatgtc
     2161 gaaggtaaga ttgcccttat tgaacgtggc gatattgatt tcaaagataa gattgcaaac
     2221 gctaaaaaag ctggtgctgt aggggtcttg atctatgaca atcaagacaa gggcttcccg
     2281 attgaattgc caaatgttga ccagatgcct gcggccttta tcagtcgaag agacggtctc
     2341 ttattaaaag acaatccccc aaaaaccatt accttcaatg cgacacctaa ggtattgcca
     2401 acagcaagtg gcaccaaact aagccgcttc tcaagctggg gtctgacagc tgacggcaat
     2461 attaagccgg atattgcagc acccggccaa gatattttgt catcagtggc taacaacaag
     2521 tatgccaaac tttctggaac tagtatgtct gcaccattgg tagcgggtat catgggactg
     2581 ttgcaaaagc aatatgagac acagtatcct gatatgacac catcagagcg tcttgattta
     2641 gctaagaaag tattgatgag ctcagcaact gccctatatg atgaagatga aaaagcttat
     2701 ttttctcctc gccaacaggg agcaggagca gtcgatgcta aaaaagcttc agcagcaacg
     2761 atgtatgtaa cagataagga caatacctca agcaaggttc acctgaacaa tgtttctgat
     2821 aaatttgaag taacagtaac agttcacaac aaatctgata aacctcaaga gttgtattac
     2881 caagtaactg ttcaaacaga taaagtagat ggaaaacact ttgccttggc tcctaaagca
     2941 ttgtatgaga catcatggca aaaaatcaca attccagcca atagcagcaa acaagtcacc
     3001 gttccaatcg atgctagtcg atttagcaag gacttgcttg cccaaatgaa aaatggctat
     3061 ttcttagaag gttttgttcg tttcaaacaa gatcctacaa aagaagagct tatgagcatt
     3121 ccatatattg gtttccgagg tgattttggc aatctgtcag ccttagaaaa accaatctat
     3181 gatagcaaag acggtagcag ctactatcat gaagcaaata gtgatgccaa agaccaatta
     3241 gatggtgatg gattacagtt ttacgctctg aaaaataact ttacagcact taccacagag
     3301 tctaacccat ggacgattat taaagctgtc aaagaagggg ttgaaaacat agaggatatc
     3361 gaatcttcag agatcacaga aaccattttt gcaggtactt ttgcaaaaca agacgatgat
     3421 agccactact atatccaccg tcacgctaat ggcaaaccat atgctgcgat ctctccaaat
     3481 ggggacggta acagagatta tgtccaattc caaggtactt tcttgcgtaa tgctaaaaac
     3541 cttgtggctg aagtcttgga caaagaagga aatgttgttt ggacaagtga ggtaaccgag
     3601 caagttgtta aaaactacaa caatgacttg gcaagcacac ttggttcaac ccgttttgaa
     3661 aaaacgcgtt gggacggtaa agataaagac ggcaaagttg ttgctaacgg aacctacacc
     3721 tatcgtgttc gctacacgcc gattagctca ggtgcaaaag aacaacacac tgattttgat
     3781 gtgattgtag acaatacgac acctgaagtc gcaacatcgg caacattctc aacagaagat
     3841 agtcgtttga cacttgcatc taaaccaaaa accagccaac cggtttaccg tgagcgtatt
     3901 gcttacactt atatggatga ggatctgcca acaacagagt atatttctcc aaatgaagat
     3961 ggtaccttta ctcttcctga agaggctgaa acaatggaag gcgctactgt tccattgaaa
     4021 atgtcagact ttacttatgt tgttgaagat atggctggta acatcactta tacaccagtg
     4081 actaagctat tggagggcca ctctaataag ccagaacaag acggttcaga tcaagcacca
     4141 gacaaaaaac cagaagctaa accagaacaa gacggttcag gtcaaacacc agataaaaaa
     4201 aaagaaacta aaccagaaaa agatagttca ggtcaaacac caggtaaaac tcctcaaaaa
     4261 ggtcaatctt ctcgtactct agagaaacga tcttctaagc gtgctttagc tacaaaagca
     4321 tcaacaagag atcagttacc aacgactaat gacaaggata caaatcgttt acatctcctt
     4381 aagttagtta tgaccacttt cttcttggga ttagcagctc atatatttaa aacaaaacgc
     4441 caaaaagaaa ctaaaaaata gtattactta aactgataag agcttattta atagagagaa
     4501 ccaatttggt tctctctatt tt
//
LOCUS       DATROY010000131         4455 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.132, whole genome shotgun sequence.
ACCESSION   DATROY010000131 DATROY010000000
VERSION     DATROY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            60..932
                     /locus_tag="VBD11_001740"
     CDS             60..932
                     /locus_tag="VBD11_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6954660.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            998..2617
                     /locus_tag="VBD11_001741"
     CDS             998..2617
                     /locus_tag="VBD11_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6954661.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            2740..>4455
                     /locus_tag="VBD11_001742"
     CDS             2740..>4455
                     /locus_tag="VBD11_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6954662.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DSFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKVGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILI
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFD"
BASE COUNT         1390 a          742 c          754 g         1569 t
ORIGIN      
        1 atttattgac agctttctct ttttatcatg ttattattac taaagttaag gagcttatca
       61 tgtctaaaaa aattaagaat tttatactat taaatattgg atctctaatt gttgcatttg
      121 gtttcaacgc tttctttctt cataatcata ttgtcacggg ggggattagt ggtttggcag
      181 ttagttttca tgaagtattt ggttgggatt ctgctttttt tattatgatt atcaatattc
      241 cattgctcct tctttgctac tttggcttag gtaaacaaac ctttttaaaa actgtctatg
      301 gttcttggat ttttcctgtt tttattaagt taacacaaag tgtaccaact ttgacccaca
      361 acccactcct cgcagcactt tttggaggtg ttattgtagg atgtggtttg gggattgttt
      421 tttggagcga ctcttcaact ggtggaacgg ggattatcat tcaattctta ggaaaatata
      481 ctcctataag ccttggacaa ggggttatat tgattgatgg acttgttaca attgttggtt
      541 tcctagcttt tgacagtgat acggttatgt tttctattat tgggttgata actattagtt
      601 atattattaa tgctatccaa actggattta caaccttaag cactgtctta atcgtttctc
      661 aagagcacca aaaaattaag acatatatca atactgtcgc agatagagga gtaacagaaa
      721 ttcccgttaa agggggatat tctggaacta atcaaatcat gcttatgaca actattgctg
      781 gttatgagtt tgctaaatta caagaggcaa tagcagaaat tgacgaaaca gccttcataa
      841 cagtaactcc aacatcacaa gcttctggac gtggatttag tcttcaaaaa aatcatggac
      901 gtcttgatga agacattctt atgccaatgt aatcgattga taattaaagc tttttctagt
      961 agaatagcag atagaatata cacaagaggt aacaaaattg cttacagttt cagatgtttc
     1021 actacgtttt agtgatcgca aattatttga tgaagttaac attaacttta cagcaggaaa
     1081 tacctatggt cttatcggag caaatggcgc tggtaaatca actttcctta aaattttagc
     1141 tggtgatatt gaaccaacaa cgggacatat tgcacttggc ccagatgaac gcctttctgt
     1201 acttcgccaa aatcattttg attatgaaga tgagcgcgtc attgatgtcg ttatcatggg
     1261 taatgaaaca ctttatagta tcatgaaaga aaaggatgct atctacatga aagaagattt
     1321 ttctgatgaa gatggtgttc gcgctgcgga attagaagga gaatttgctg aactcggtgg
     1381 atgggaggct gaaagtgagg cttctcaatt actccaaaat ctaaatattt cagaagagct
     1441 tcattatcaa aatatgagtg aattagctaa tggagacaaa gttaaagttc tccttgcaaa
     1501 agctctcttt ggtaaaccag atgtacttct ccttgacgag cctaccaatg gacttgatat
     1561 ccagtctatt acttggttag aagatttctt gattgacttt gaaaatacag ttattgtagt
     1621 ttcccacgac cgtcatttct tgaataaagt atgtacacat atggctgacc ttgattttgg
     1681 aaaaattaaa ctgttcgtcg gcaactatga tttctggaaa gaatcatcag aattagcagc
     1741 tcgtttacaa gctgatcgta atgctaaagc agaagaaaaa attaaacaac ttcaagaatt
     1801 cgttgcacga ttttcagcga atgcttcaaa atcaaaacaa gcaacttctc gtaaaaaaat
     1861 gcttgataaa attgaacttg aagagattgt accatcaagc cgtaaatacc cattcgttaa
     1921 ctttaaagcc gaacgcgaaa tgggaaatga tttattgacc gttgaaaatc tctctgtcac
     1981 tattgatggg gaaaaaatcc ttgataatat cagtttcatt cttcgtcctg gtgataaaac
     2041 tgcacttatt ggacaaaatg atatccaaac tactgcactt attcgagcat tgatggggga
     2101 tatcgaatat gaaggaacaa tcaaatgggg agtaacaact agtcgttctt accttccaaa
     2161 agacaattca cgagactttg cttctggcga gtcaatctta gaatggcttc gtcaatttgc
     2221 tagtaaagaa gaagacgata ataccttctt acgtggcttc ctaggtcgta tgcttttctc
     2281 tggagatgag gttaacaaat ctgttaatgt cttatctggg ggtgaaaaag tacgcgttat
     2341 gctttcgaaa ttaatgctat taaaatctaa tgttcttgtt cttgatgatc caacaaacca
     2401 cttagatttg gaatctattt caagtctcaa tgatggacta aaagatttca aagaatctat
     2461 tatttttgca agtcatgacc acgagtttat tcaaacttta gcgaaccata ttattattct
     2521 ttctaaaaac ggagttatcg atcgtatcga tgaaacttac gatgaatttc ttgaaaatac
     2581 cgaagtacaa gctaaggtcg ctcaactctg gaaataatcc tttggcatat ctctaaccta
     2641 ataaacgtgt caagattgaa aatcttaaaa ctatattaat catttaaata aaaaggttgg
     2701 aacaaatagc gtccagcctt tttatcttag gagatatata tgacaattaa ccactataag
     2761 cttcgtatac cttactatac aataagcttt ctacttccct tcataatcat agtctgtatc
     2821 ctctttacta agaatattta ttggggaagt ccaacaacca tattagcaag cgatagcttt
     2881 catcaatatg ttatctttaa tcaagcatta agaaatattc ttcatggttc aaacagctta
     2941 ttctatacct ttacaagcgg tttaggttta aatttttatg ctctgagtag ttactatttg
     3001 ggaagttttt tatccccaat agtttatttc tttaacctaa aaaatatgcc tgatgctatc
     3061 taccttttaa caatatgtaa agtagggttg ataggtttgt caatgtttgt cactctttgt
     3121 aaacgtcatt gtaaagttaa tcgtgtctta cttttagtaa tttctacttg ctatagctta
     3181 atgagtttct caattagcca aattgaaatt aatatgtggc tagatgtatt tattctaatt
     3241 ccccttgttg ttttaggagt tgatcagcta ctatgggaaa gaaaacctat acttcatttt
     3301 ctctctctga cagccttatt catccaaaac tattattttg gttttatgac taccatcttt
     3361 actagccttt acttcatcgt acaaataacc cgtaatactg atagtaaggt tgcatttaaa
     3421 caatttcttc attttacctt tttatctctt ttagctggta tgactagtag cattatgatt
     3481 ttgccaactt attttgattt aactactcat ggtgaaaagt tgacaaaggt atcgaagatg
     3541 tttacagaaa attcttggta catggatttg tttgctaaaa atatgattgg agcctatgat
     3601 acaacaaaat ttggctctat tcctatgatt tatgtaggtt tacttccttt actcctttct
     3661 ttactttact ttacaataaa ggaaattcct cgaaggacta gattagcata tggattttca
     3721 ctaatatttg ttattgctag tttttatata acacctctag atttattttg gcaagggatg
     3781 catgctccaa atatgtttct tcatcgctac tcttgggtac tttcagtact tatctgtcta
     3841 ctagctgctg aatgccttga gtatttagac aacatttctt ggaaaaagat tttaggggtc
     3901 aatttaattt taataagtgg tttcataatc acgtttttat ttaaaaagca ctatcattac
     3961 cttaatcttg aattacttct tttaacctta acattcctct cagcttatat tattctaact
     4021 attagttttg tcagtaagca aatccctaaa ttagtttttt atccctttct tattggtttt
     4081 gttgtgttag agatgacatt aaacaccttt taccaactaa atagtcttaa cgatgagtgg
     4141 gtatttccat ctaggcaagg atatgctaaa tataatcaca gcattagcaa acttgtcaga
     4201 aagactgaga gaaataactc tacattcttc cgtacagagc gctggttagg acaaaccggc
     4261 aacgactcta tgaaatataa ttataatggt atttcacaat tttcatctat tagaaatcgt
     4321 tcttcaagtc aagtgcttga tagacttggc tttaaatcag atggtacaaa tttaaattta
     4381 cgttatcaaa ataatacttt gatcgcagat agtctatttg gtgtaaagta caaccttaca
     4441 gaatatcctt ttgat
//
LOCUS       DATROY010000132         4415 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.133, whole genome shotgun sequence.
ACCESSION   DATROY010000132 DATROY010000000
VERSION     DATROY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..97)
                     /locus_tag="VBD11_001743"
     CDS             complement(<1..97)
                     /locus_tag="VBD11_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6954663.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNL"
     gene            300..2339
                     /locus_tag="VBD11_001744"
     CDS             300..2339
                     /locus_tag="VBD11_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6954664.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSSYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2449..3027
                     /locus_tag="VBD11_001745"
     CDS             2449..3027
                     /locus_tag="VBD11_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6954665.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(3061..4302)
                     /locus_tag="VBD11_001746"
     CDS             complement(3061..4302)
                     /locus_tag="VBD11_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6954666.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
BASE COUNT         1492 a          783 c          808 g         1332 t
ORIGIN      
        1 gtagattaag cccgatggct ttaaaggttt ctcctctatc caaaccagat tggatgtcat
       61 tacggtctga gagagttagg tgtttgtgtt ttgtcatgat ggataaactc atttctaacc
      121 caaacgtctc agacttaatt ccaccataaa aatccttttc acactaactt ccggttttgg
      181 agggcagacg gaacttactt tgagaaatta cttaatataa aataacagtt aatattttag
      241 caatttgata acacttagat tataatgtaa acgtatactt ttttagattg gaagatgaaa
      301 tgaaacgtaa atactttgtt cttaatacgg tgacggtttt aacgttagct actgcaatga
      361 atactagcag tagctatgct aatagtactg agacaagtgc ttcagtagct cctactacaa
      421 atactatcgt tcaaactaat gacagtaatc ctaccgcaaa atttgcatca gaatcaggac
      481 aatctgtaat aggtcaagta aaaccagcta attctgcggc gcttacaaca gttgacacgc
      541 ctcatatttc agctccagat gctttaaaaa caactcaatc aagtcctgtc gttgagagtc
      601 cttctactaa gttaactgaa gagacataca aacaaaaaga tggtcaagat ttagccaaca
      661 tggtgagaag tggtcaagtt actagtgagg aactcgtcaa tatggcatac gatattatcg
      721 ctaaagaaaa cccatcttta aatgcagtca ttactactag acgccaagaa gccattgaag
      781 aggctagaaa acttaaagat actaatcagc cgtttttagg tgttcccttg ttagtcaagg
      841 ggttagggca cagtattaaa ggtggtgaaa ccaataatgg cttgatctat gcagatggaa
      901 aaattagcac atttgacagt agctatgtca aaaaatataa agatttagga tttattattt
      961 taggacaaac gaattttcca gagtatgggt ggcgtaatat aacagactct aaattatacg
     1021 gtccaacgca taatccttgg aatcttgctc ataacgctgg tggctcttct ggtggaagtg
     1081 cagcagctat tgctagcgga atgacgccaa ttgctagcgg cagtgatgct ggtggttcta
     1141 tccgtattcc atcttcttgg acgggcttag taggtttaaa accaacaaga ggattggtga
     1201 gtaatgaaaa gccagattcg tatagtacag cagttcattt tccattaact aagtcatcta
     1261 gagacgcaga aacattgtta acttacctaa agaaaagcga tcaaacgcta gtatcagtta
     1321 atgatttaaa atctttacca attgcttata ctttgaaatc accaatggga acagaagtta
     1381 gtcaagatgc taaaaatgct attatggaca acgtcacatt cttaagaaaa caaggattca
     1441 aagtgacaga gatagattta ccaattgatg gtagagcatt aatgcgtgat tattcaacct
     1501 tggctattgg catgggagga gctttttcaa caattgaaaa agacttaaaa aaacatggtt
     1561 ttactaaaga agacgttgat cccattactt gggcagttca tgttatttat caaaattcag
     1621 ataaggctga acttaagaaa tctattgtgg aagcccaaaa acatatggat gattatcgta
     1681 aggcaatgga gaagcttcac aagcaatttc ctattttctt atcgccaacg accgcaagtt
     1741 tagcccctct aaatacagat ccatatgtaa cagagaaaga taaaagagcg atttataata
     1801 tggaaaactt gagccaagaa gaaagaattg ctctctttaa tcgccagtgg gagcctatgt
     1861 tgcgtagaac accttttaca ccaattgcta atatgacagg actcccagct atcagtatcc
     1921 cgacttactt atctgagtct ggtttaccca tagggacgat gttaatggca ggtgcaaact
     1981 atgatatggt attaattaaa tttgcaactt tctttgaaaa acatcatggt tttaatgtta
     2041 aatggcaaag aataatagat aaagaagtga aaccatctgc tgacctaata cagcctacta
     2101 actccctctt taaagctcat tcatcattag taaatttaga agaaaattca caagttactc
     2161 aagtatctat ctctaaaaaa tggatgaaat cgtctgttaa aaataaacca tccgtaatgg
     2221 catatcaaaa agcacttcct aaaacaggtg atacagaatc aagtctatct ccagttttag
     2281 tagtaaccct tttattagct tgttttagct ttgtaacaaa aaagaatcag aaaagttgat
     2341 tgtaatgtaa aataattcct atttttcact ttttagtatc atattttcga tttttatgtt
     2401 gtaataaatt ataatgaaga tgtaaggaat tataaaatag gaaataatat gaaagtgaaa
     2461 aataagattt taacgatggt agcacttact gtcttaacat gtgctactta ttcatcaatc
     2521 ggttatgctg atacaagtga taagaatact gacacgagtg tcgtgactac gaccttatct
     2581 gaggagaaaa gatcagatga actagaccag tctagtactg gttcttcttc tgaaaatgaa
     2641 tcaagttcat caagtgaacc agaaacaaat ccctcaacta atccacctac aacagaacca
     2701 tcgcaaccct cacctagtga agagaacaag cctgatggga gcacgaagac agaaattggc
     2761 aataataagg atatttctag tggaacaaaa gtattaattt cagaagatag tattaagaat
     2821 tttagtaaag caagtagtga tcaagaagaa gtggatcgcg atgaatcatc atcttcaaaa
     2881 gcaaatgatg agaaaaaagg ccacagtaag cctaaaaagg aacttcctaa aacaggagat
     2941 agccactcag atactgtaat agcatctacg ggagggatta ttctgttatc attaagtctt
     3001 tacaataaga aaatgaaact ttattaaata taaaaaggag gactaacctc ctttttatat
     3061 ttaaatagcc cattcgccat ttctaaagat tgggacagca gttccatctg cacgaatacc
     3121 atcaatatcc atttgttctg aaccaatcat aaaatcaaca tgtgttgaag aacgatttaa
     3181 accagcttca tccaattctt gacttgtcat ttctgttcct ccttctacat tgaaggcata
     3241 tgcagatcca atagcgaggt gattagaggc attttcatcg aataaagtat taaagaaaat
     3301 tagtccagac agtgaaattg gtgttttatg tgggacaagt gcaacttccc caagcgatct
     3361 tgccccatca ttttcctcga tcaagcgttg gattgtttct tgcccttttt ctgcagtgac
     3421 attgataatt ttaccgtctt taaaagtaaa cgtcatattt tcgataataa cgccagcata
     3481 actgagaggt tttgtacttg tcacataccc atctgcacga cggtaatcag gtgctgaaaa
     3541 gatttcttca gtaggcatat tagcgataaa agtctctccc tgagcgttga gactaccggc
     3601 tgcctcccaa atgtgatttt taggcatacc aagcgttaaa tctgtacctg gtgcggtata
     3661 atgaagagca tcaaattgtt cttgatttag tctagtagct ttttctaata atttagcttc
     3721 atgtgcatcc caagcagcaa taggattatc ttcataaata cgatttaatt tgaaaatggt
     3781 atcccaaaga gcatcaactt gttggtcgct tgtttttaat tctgggaaga caagtgcagc
     3841 ccattctcta ccagcagctg cagccacgtt ccaagatact ttattagctt gtattgctct
     3901 actttgttcc tctaaggcaa tagcggttgc tctagttgtt ttagataaac gtttaggatc
     3961 aacagtagcg aaagcgttag ggttagaaga tttcacaact aatcgactag ccttcttatt
     4021 taatagcgct aaagatttat caacggtata ctgtggaaca ttcgtgagaa cttcgtctga
     4081 agcatgaagt agtcgctggc gcgtgataaa atcatctgta taatcaacaa taacttcact
     4141 tgctcccttt tcataagcga cttcagttaa aagccttgct aagtggactt gttccacatc
     4201 gattgttaaa gcgagagtgt gccctttttg gacatttaaa cctttagaaa taattaattg
     4261 ggcatatttt tttaaaaggt tgtcgaaatc ttgtaaaacc atactagctc ctttaaattt
     4321 actatttttt ataatttatc ataaactctt ataaagcaca aggaaatatg gaagcgattt
     4381 aataaagata tatttaagct aacggtttgt agttt
//
LOCUS       DATROY010000133         4390 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.134, whole genome shotgun sequence.
ACCESSION   DATROY010000133 DATROY010000000
VERSION     DATROY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            9..233
                     /locus_tag="VBD11_001747"
     CDS             9..233
                     /locus_tag="VBD11_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO6954667.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            311..2470
                     /gene="nrdE"
                     /locus_tag="VBD11_001748"
     CDS             311..2470
                     /gene="nrdE"
                     /locus_tag="VBD11_001748"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6954668.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            2673..3632
                     /gene="nrdF"
                     /locus_tag="VBD11_001749"
     CDS             2673..3632
                     /gene="nrdF"
                     /locus_tag="VBD11_001749"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6954669.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3717..3815
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3872..>4390
                     /gene="thiT"
                     /locus_tag="VBD11_001750"
     CDS             3872..>4390
                     /gene="thiT"
                     /locus_tag="VBD11_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6954670.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISII"
BASE COUNT         1436 a          786 c          746 g         1422 t
ORIGIN      
        1 aggacaacat ggcagctatt actgtttttt cgaaaaacaa ctgtatgcaa tgtaaaatga
       61 ctaagaaatt tcttgaccag cacggcgctg attttgaaga aattaatatt gatgaaaaac
      121 cagaaaaaat tgaatatgtg aaaaatctcg gcttttctgc ggctccagtg attgaagctg
      181 gtaatgttgt tttttcaggt ttccaaccaa gcaaactaaa agaacttgtt taagcaaatt
      241 agagtctggg aataaagcaa ttaattgctt tattcccatt ttgtattgtt aagttaagag
      301 gataaattca atgagtttaa aaaatattgg cgatgtatct tactttcgcc taaataatga
      361 aattaaccgt cctgttaatg gacaaatccc actccataaa gataaagaag ccttaactgc
      421 ttttttcaaa gaaaacgtac agcctaattc aaaagctttt gactcaatca ctgataaaat
      481 tgcttacctc cttaagtatg attatcttga agaagctttc ctcaataaat atcgtcctga
      541 atttattgaa gaattatcta ccaaactctt cgataaaaag tttcgtttta aatccttcat
      601 ggcagcctat aagttttatc agcagtatgc attaaaaaca aatgatggag aatactattt
      661 agaaagtatt gaagatcgcg ttctctttaa cgctctttat tttgcagacg gtgatgaaga
      721 actagcaact gacttagctc tagaaatgat ttcacaacgc tatcaacctg caacaccaag
      781 ttttttaaat gctggtcgaa gtcgtcgcgg agaattagtc tcatgtttct taatccaagt
      841 aactgatgat atgaatgcta ttggtcgttc gattaattca gctcttcaat tatctcgtat
      901 tggtggtggg gttggaatat cacttagtaa ccttcgtgag gctggagctc ctatcaaagg
      961 ttttgctggt gctgcttctg gagtcgttcc agtcatgaaa ttatttgaag acagtttttc
     1021 ttactccaac caacttggac aacgccaagg tgctggagtt gtttaccttg acgttttcca
     1081 tccagatatt atttcattcc tatcaactaa aaaagaaaat gctgatgaaa aagtacgtgt
     1141 taaaacattg tcattaggga ttactgtacc cgataaattc tatgaactag cacgtaacaa
     1201 tcaagaaatg tacttgttta gtccttatag tatcgaacgt gaatatggtg ttcctttcag
     1261 ctatattgat attaccgaaa aatatgatga attagtcgct aaccctaata tcactaaaac
     1321 aaaaatcaat gctcgtgatt tagaaacaga gatttctaaa cttcaacaag aatctggtta
     1381 tccttacatc atcaatattg acactgctaa tcgtactaat cctgttgacg gaaaaattat
     1441 tatgtcaaat ctttgctctg agattttgca agtacaaaaa ccaagtctta tcaatgatgc
     1501 tcaagaatac cttgaaatgg gaactgatat ttcttgtaac cttggctcaa ctaatgtttt
     1561 aaatatgatg acatctcctg attttggaaa atcaattaaa acaatgactc gtgctctaac
     1621 tttcgttact gactcatcaa atattgaagc tgttccaact attaagaacg gaaatgctca
     1681 agcacataca tttggacttg gagcaatggg actccactcc tatttggcaa aaaatcatat
     1741 cgaatatggt agtccagaat caattgaatt tactgatatt tatttcatgc taatgaacta
     1801 ctggacctta gtagaatcaa acaatattgc ccgcgaacgt caaacaacat ttgtaggttt
     1861 tgaaaaatca aaatacgctg acggtacata ctttgataaa tatgtatctg gaaaatttgt
     1921 tccacaatct gacaaagtaa aatccctatt tactaatcat tttatccccg aagctaaaga
     1981 ttgggaaaac cttagatacg cagttatgaa agacggacta tatcatcaaa atcggcttgc
     2041 agttgctccg aatggttcta tttcgtatat caacgattgt tcagcatcta tccaccctat
     2101 tacacaacgt attgaagagc gtcaagagaa aaaaatcggg aaaatttatt atccagctaa
     2161 tggcttagct acagatacta tcccttatta cacttctgcc tatgatatgg atatgcgtaa
     2221 agtaatagac gtctatgccg cagcaactga gcacgtagac caaggtctct caatgacact
     2281 ctttttacga agcgaattac caaaggagtt gtacgagtgg aaaacagaaa gtaagcagac
     2341 tactcgcgac ctttcaatcc tacgtaacta tgctttcaac aaaggtgtga aatcaatcta
     2401 ctatatccgt acttttacag atgatagtag cgaagtcgga gctaatcaat gcgaaagttg
     2461 tgtcatctaa cgaagaaact ctacttaaaa catcagaaac tagactgaga taaactatct
     2521 cagtttcatg atgttatctt attttctaag gtattttcat ctttattttt ctaaaaatcg
     2581 ataatacttg aaatattgct agatcagaat tctatattct actcttcttc catcatcatt
     2641 atataaaaac ctatttacca ttggagaaaa ccatgacaac ttactacgaa gctataaact
     2701 ggaacgaaat tgaagatgtt attgataaat caacttggga aaaactaacc gaacaatttt
     2761 ggctcgatac acgtatccct ttatcaaatg acttagacga ttggcgcaaa ctttccgctc
     2821 aagaaaaaga tcttgttggc aaggtttttg gaggcttaac cctacttgat accatgcaat
     2881 cagaaactgg tgttgaagct attcgtgccg atgttcgcac gcctcacgaa gaagctgtct
     2941 taaacaatat tcaattcatg gaatctgttc acgctaaatc ttattcttca attttctcaa
     3001 ctttaaatac taaatcagaa attgaagaaa ttttcgagtg gactaataat aatgagttcc
     3061 ttcaagaaaa agcacgtatt atcaatgaca tttatgctaa tggaaatgcc cttcaaaaaa
     3121 aggtggcttc cacctacctc gaaactttcc ttttttattc tggctttttc acacctcttt
     3181 actatttggg aaataataag ttagcaaatg ttgctgaaat cattaaatta attattcgtg
     3241 atgaatctgt acatggtact tatatcggtt acaaattcca gcttggtttt aacgaattac
     3301 cagaagatga gcaagagaat tttcgtgatt ggatgtatga cctcctttat cagctgtatg
     3361 aaaacgaaga aaaatacacc aagacacttt atgatggcgt aggatggact gaagaagtta
     3421 tgaccttttt acgctacaat gctaataaag ctcttatgaa tttaggacaa gatcctttat
     3481 tcccagatac agcaaatgat gtcaacccaa ttgttatgaa tggtatttca acaggaacat
     3541 caaaccatga cttcttctct caagtaggta atggttacct acttggtagc gttgaagcta
     3601 tgcatgatga tgactataac tatggattat aagaaaaaga cactgattta gtgtcttttt
     3661 ctagttaaat acaaatttct tgacaagcaa accctttcaa gatagaatgt tagagtaaat
     3721 acacaaggga gtgccttgag ctgagattgc agatatgcaa aatcctctaa cctgatctcg
     3781 ttaggacgag cgtaggaatt gtggtttgaa ttggttaaac atgacaaatg acaaaagaat
     3841 ctcctttgct gtataaaaaa ggagattttt tatgtcaaaa aataacaaca ctacctgtct
     3901 tattgaaact gctatttttg cagcgcttgc tatggcttta tcaatgattc ctgattttgc
     3961 gtcatggttc actccctctt ttggagcaat tcctctgata ctttttgcat tacgtcgtgg
     4021 tacaaaatac ggtctatttg ctggcttaat ttggggctta ttgcactttg ttctttccaa
     4081 agtatattac cttagtttat cccaagtttt tatagaatac atcttagcat ttataagtat
     4141 gggacttgca ggcgtatttt ctgctaaatt taaagatgca cttagcagca gctctaaaac
     4201 taaagcttta tcattagcat tatctggagc aatattagca acatttgttc gttatgtttg
     4261 gcattatatc gctggtgtta ttttttgggc tagttatgca ccaaaaggta tgtctgctac
     4321 attatactca ttaagcgtca atggtactgc tggtttgtta actctatttt ttgtagtcat
     4381 tagtattatt
//
LOCUS       DATROY010000134         4370 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.135, whole genome shotgun sequence.
ACCESSION   DATROY010000134 DATROY010000000
VERSION     DATROY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(28..192)
                     /locus_tag="VBD11_001751"
     CDS             complement(28..192)
                     /locus_tag="VBD11_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954671.1"
                     /translation="MLLTSIDVNPVYANMAFDWVYDNYKDDIINYGQETIKNIGKSIS
                     ANNSLGTLYN"
     gene            complement(494..946)
                     /locus_tag="VBD11_001752"
     CDS             complement(494..946)
                     /locus_tag="VBD11_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6954672.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            complement(958..2025)
                     /locus_tag="VBD11_001753"
     CDS             complement(958..2025)
                     /locus_tag="VBD11_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6954673.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            complement(2125..2841)
                     /locus_tag="VBD11_001754"
     CDS             complement(2125..2841)
                     /locus_tag="VBD11_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6954674.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            complement(2843..4342)
                     /locus_tag="VBD11_001755"
     CDS             complement(2843..4342)
                     /locus_tag="VBD11_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6954675.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
BASE COUNT         1422 a          890 c          684 g         1374 t
ORIGIN      
        1 tctatttccg ttttcaaatc ttgctcctta attatataaa gtacctaaac tattattagc
       61 tgaaatggat ttaccaatat ttttgatagt ttcttgtcca taattgatga tatcatcttt
      121 atagttatca tacacccaat caaatgccat attagcataa actggattta catctattga
      181 ggtcagcaac aatatctttt ctcttttttg taaattattt ttgcaattcg agattcccag
      241 cagtactaat tttagatgtt gataatagat ggatcagcta ttctattatc aacatctaaa
      301 actttctgtg aaaaatcatt aggaataatg actattaact ggtgatcacc ttttttaggc
      361 cattttcagt aatcccatga ctaacaattg accaattttg agaattatct cgttcaatat
      421 atttaacata actggccctt agcttaaacg ataaaacctt caaggaagat aggcatatac
      481 tctatcttcc tctttactct tccatcccta gtgttacctc tggaacatca tgttgaacat
      541 aagcaactcc ttttgcttcc ataagttcaa tagcaaatgg gtgtgggcga taattagctt
      601 tataagtaat tttcttaacc ccggcttgca agagcgcctt ggtgcaattg atacaaggaa
      661 aatgcgtcac ataaatttct gtattattag tagaaatacc ttctttagca cactgaataa
      721 gggcattcat ctcagcatga acagtcctaa tacagtgacc atcttccata taatgtccca
      781 cttcgttaca gttatctgtc tcagagactc ctccgttata acctgtagca atgatgcgat
      841 tatttttgac taaaacagct ccaacaaaag cacggtcaca ggtagaacgt ttagaaatca
      901 actctgcatt tgccataaaa taatcttccc aagaaagtct attcattcta ttttccttta
      961 aagtacaatt aattctttcg gtgcagatgt caatacttcg caacctgttt ccgttattaa
     1021 taaatcatct tcaatacgaa caccgtattt attatcgaga tatattcctg gctcgtcagt
     1081 cactaccatc ccagatgcaa tcactccctc tgacttacca aaataaggaa tttcatgaac
     1141 atcaagcccc ataccatgcc ctataccgtg ggtaaaatga ttaccgtatc cagaattttc
     1201 aatgacttgt cgcgccaagt aatcataatc acaacgcttc attcctgatt ttacattatc
     1261 aataatagct tgattacttt tcaaaacgat atcgtaaatc tcgcgttcct gatcagtgac
     1321 atgaccgata tgaatcgtac gtgtcatatc actaacatag tgctgatagt aacaaccaaa
     1381 atctaaagtc agagtttctc ccgattgaat agtcttctga ctcgcaaccc catgtggcat
     1441 cgctgatcga tatccagatg ctacaataaa atcaaatgaa ggaccagttg ctcctaattc
     1501 tctcatgcga aagtctaaaa aattagcaac ttgcaattca gttgttcgat caggctttat
     1561 aaaatccaac acatctaaaa aagcttggtc cgaaatacga caggcttgac ggatgatcgc
     1621 aagttctgtt acatctttaa tcatacgtaa cttttcaaca acagatggag cttctatcaa
     1681 gcgataatct tcaaaaactt ccaccatatg tttgtacata tgataagaaa tatcagtttc
     1741 aaaaccaatc gctatcaatg cgtcatcgcg aataattttc aaaatagccg caagtggttc
     1801 tctgctaatg ataatatcaa actctcttac agtattcttt gctatcaagg catatcgtga
     1861 atctgtaaca aaaatccggt gttttggact aatgagtacc gttgcattag ttcctgaaaa
     1921 accagtcaaa taaaaaatat ttgttaaatt tgttattaag agtgcgtcac atgataattg
     1981 tgaaattgct gtttgacatc tagttagacg ttgttcaaaa cccataagaa acctcgctat
     2041 tctaacctta attaggttaa ttttatcatc ttttaaaaca tttagctata agaaaagcaa
     2101 ctcatttcag ttgcttttac tttactattc ttttatatta agagcttctt ttaatgcttt
     2161 aacttgcatc tcagcataag cttctggatt tgtcttagct aatgtcttta tctccatagc
     2221 tttcactggg tttacttttg cttgcgaagt catttgttct tttagtttag ccgcagccga
     2281 ttctaagtgt tcggactgct ctttagtcaa aactagccaa gtttttcgtg gtattttagc
     2341 aactgatttc tgagaaatca attctccatt ttcacctgca aagccataaa gtagtttcga
     2401 aagactatct ttccacacat aacgtttagt ctttgttgtt actgtggctt ttttcacatc
     2461 agctaattgg tagctagcag attttttcac agcacttgtc acttcttttg tatttggaac
     2521 aaagtgagct tttgcttctt tttgatttaa atctgttcga taaactaaaa cattattact
     2581 cgaatgagta ccaatctctt tataaatcat gagaccaaat ggagccttac tatcacccgc
     2641 actataaatc tcacgtgtct ttgtcttagt ttcaatagtc atacctgtat gattaataaa
     2701 gtgatccgtt aacatccaaa ctgaggcaaa caacaaagct aatgagagaa gacctagaat
     2761 ataccgtaga gcttttggac gaatcaacat ccatgtgaca aatgtcaaaa tagttgccag
     2821 tgcaataatc aaaataatca tgctatacct ccttttccgt ctcaataatt ttcccttttc
     2881 tcaggaaaag agataccagt aaaccaagaa ccgcaaatac aaaaccaatg gcaaaagaaa
     2941 catggaagcc atcaagacta gcattcaaca tctgatttgc atattttaaa ggattcattg
     3001 ttagcaaatc ttttgatggt ttattgttgg tgataatatt ttgagcaaca cttgaaagca
     3061 atgcaactac aactgctgaa gcaatttgac gagcagtatt attagctgct gttccatgtg
     3121 cggcctcatg tggtggaagt gcgctcatcg cacttgctgt caatggcatc ataaccatag
     3181 caataccaaa catacgtact gcatataaga gggtaataaa atgatctggt gttgtagtcg
     3241 ttaagaaaac aaaaggtaac gtcgctacac ctagtatggt aaaaccaatc atagccatac
     3301 gtcttgcacc aactttatca tatacagctc cagaaattgg actaactatc cccatcatca
     3361 aggcacctgg taataaagct aaaccagaat caagtgcaga gagaccatga acattttgca
     3421 agtaaagcgg tagcatcatt tcaacaccca tcatcgccat cattgaaagt gcaatagctg
     3481 ctgttgttac tgagaattgt ttatttttga aaacacggat atctaagaac ggtgtttcta
     3541 gttttaattg acgatgaatg aataaggcga taaaaataat accaaccata ataggagaaa
     3601 taacatatcc aatatctccc catccataag ttgccacatt tgtgaagccc cacaaaaagc
     3661 taccaaagcc aattattgaa aggataaggg aaggcacttc caatttaact gacttattat
     3721 caaccacatc ttttagaaca aagggggata aaattgtagt gaccgttaaa attagtaaag
     3781 gaagaaggaa aattgcacgc catgttaaat catgccctgc aaaatgaaat tcttgtttta
     3841 aaatccaccc tgctaatgta ggcccaatag ctggggcaag accaaccacc aaaccattaa
     3901 gtcccatagc ggcaccacgc tgctctggag gaaaaacatt caccataaca acctgcatca
     3961 aaggcataga aatccctaca gaaatcgctt gaataatacg tcctactaaa aaaaggttcc
     4021 agttagatgt aggtgctaac gtcgtcatta acagtccaat aagtaaaaca acgtaggatg
     4081 tcacatacaa ccattttgtt gaaaaacgtg tcgctaaata agctgaaaca ggaatcataa
     4141 tcccattcgc taataaaaac catgttgttg cttgttgagc cgttgacaac gaaatgttaa
     4201 agctattcat caaagttggg atagccgttc ccaaacttgt ctgatttaat acccctgcaa
     4261 atgtagcaat caacaataag gtgatcatgg ctgtacgatt atatggtttg ccatgaatat
     4321 catgttttgc ttttgtagtc attttatctc caatctctat ttctgatttt
//
LOCUS       DATROY010000135         4337 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.136, whole genome shotgun sequence.
ACCESSION   DATROY010000135 DATROY010000000
VERSION     DATROY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            19..867
                     /gene="rgg2"
                     /locus_tag="VBD11_001756"
     CDS             19..867
                     /gene="rgg2"
                     /locus_tag="VBD11_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEO6954676.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            1016..1837
                     /gene="mutM"
                     /locus_tag="VBD11_001757"
     CDS             1016..1837
                     /gene="mutM"
                     /locus_tag="VBD11_001757"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6954677.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            1828..2421
                     /locus_tag="VBD11_001758"
     CDS             1828..2421
                     /locus_tag="VBD11_001758"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6954678.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            2545..3246
                     /locus_tag="VBD11_001759"
     CDS             2545..3246
                     /locus_tag="VBD11_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954679.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            3471..>4337
                     /locus_tag="VBD11_001760"
     CDS             3471..>4337
                     /locus_tag="VBD11_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954680.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFL"
BASE COUNT         1484 a          627 c          803 g         1423 t
ORIGIN      
        1 gttgtgaaag tgggaaaaat ggaaaaagaa ttaggaaaaa cactaagaag attacgtaaa
       61 ggaaaaaaag ttagtatttc ttctttagca gatgagcacc tatccaagtc acaaatctca
      121 aggtttgaga gaggagagtc agaaattact tgtagtcgtt tgcttaatat tttagacaaa
      181 ctcaacatca ctattgatga atttgtgtcg attcattcta aggcgcacac tcattttttt
      241 atccttttaa atcgagtaag aaaatattgt gctgaaaaaa atgtaactaa attagtggct
      301 ctcttagagg atcataacca taaagactat gaaaagatta tgattaaggc tcttattttt
      361 tcaattgatc aatcaataga gcctaatcaa gaagaattag cacgcttaac agactatctt
      421 tttacggtgg agcagtgggg atattatgaa attattctct taggaaattg ctcacgtctc
      481 attaattaca atactttatt tttattgacc aaagaaatgg ttaattcgtt tgcttattca
      541 gaacaaaata aaaccaataa aatcctagtt acacacttag caattaattg tctaatcatc
      601 agcattgatc actcttactt tgagcatagc cactacttaa ttgataaggt tagatcattg
      661 ttacaggatg aagttaattt ctatgaaaaa actgttttct tatatgtaac tggatactat
      721 catttaaaac ttggtgatac ttcttcagga aaagaagata tgaggaaagc tttacagatt
      781 tttaaatatt taggtgaaga ttcattttac aatacttata atgaacatta ttgtcaaaaa
      841 gtacttggca ataataaaga atgctaatga gtgcaagttt atgagtaatt tttaaagctt
      901 ttgtctcaaa atgataataa gaaaggaaaa tagaaatcga atggtcaggt gacatcaaga
      961 acgtttggaa attattgctc taagcaatga ctattgattt ttagactata tatttatgcc
     1021 agaattacca gaagtagaaa ccgttagaaa aggactagaa agacttgtag ttaatcaaga
     1081 aatagcttct ataacgatta aagtccctaa aatggtgaaa actgatttga atgactttat
     1141 gataagtcta ccaggaaaaa caattcaaca ggtattgaga cgagggaagt atcttctttt
     1201 tgattttggt gaaatggtta tggtatcaca tttacgcatg gaaggaaaat atctactctt
     1261 cccaaataaa gtaccagata acaaacattt tcacctttat ttcaaattga ctaatggcag
     1321 cacattggtt taccaagatg tccgtaaatt tgggacattt gagttggttc gtaaatcatc
     1381 tctcaaagat tattttactc aaaaaaaatt aggtcctgag ccaactgctg acacatttca
     1441 atttgagcct ttttcaaaag gcttagcaaa ctccaaaaag ccaataaaac ctttactatt
     1501 agatcagaga ttagtagcag gacttggaaa tatttacgta gatgaagtct tgtgggcagc
     1561 aaaaattcac ccacagcgtt tggctaatca attgaccgaa tctgaaacaa gcttattaca
     1621 caaggaaatt attcgcattt taactttagg catcgaaaaa ggtggctcca cgattagaac
     1681 atataagaat gctcttggag aggacggtac gatgcaaaag tatttacagg tctatggtaa
     1741 aactgggcaa ccgtgtccaa ggtgtggttg tttaattaag aagataaaag taggagggag
     1801 agggacgcat tattgccctc ggtgtcaatg cttatgacaa aaataatagg actgacagga
     1861 gggatagctt ctggaaagtc aacggtaaca aaaataatac gagaatcagg ttttaaagtc
     1921 atagatgcgg atcaagtggt tcataaattg caagctaagg gtgggaaact ttaccaagct
     1981 ttattagaat ggttgggtcc cgagatactt gatgctgatg gtgagttgga tagaccaaag
     2041 ctttctcaaa tgatttttgc taatccagac aatatgaaga catcagctag gctacaaaat
     2101 agtatcattc gtcaagagtt agcatgtcag cgcgaccaat taaaacaaac agaagagata
     2161 tttttcatgg atattccttt attgattgaa gaaaagtata taaaatggtt tgatgagatt
     2221 tggttggtat ttgttgataa agaaaaacaa ttacaacgat taatggcccg taacaactac
     2281 agtcgagaag aagcagaatt acgactttca caccaaatgc ctttaacaga taaaaaaagt
     2341 ttcgctagtc ttattattga caataatggt gatttaataa ctttaaaaga gcaaatattg
     2401 gatgctcttc aacgtttata gaactactaa atggataatg tatttatcca ttttttgtct
     2461 ttattacact agttttaaaa aacttgcgat taaaccacag taatagtaca atgatataaa
     2521 aggtttactt tcaaaggagt ttttatggag ttagtaatta gagatattcg taagcggttt
     2581 caggaaacag aggtcttgag aggagcaagt taccgatttt attcaggtaa aataacaggg
     2641 gtcttaggta ggaatggtgc tgggaaaaca actttattta atatacttta tggggatctt
     2701 gcagctgaca acgggaccat ttgtttattg aaggataatc acgagtatcc tcttaccgat
     2761 aaggatattg gtattgttta ttccgaaaac taccttccag aatttttaac agggtatgaa
     2821 tttgtaaaat tttacatgga tttacatcct tcagatgatt taatgacaat agatgattat
     2881 ttagatttta tggaaatagg acaaacagag cgtcatagaa ttatcaaagg atattctgat
     2941 ggaatgaaga gtaagctctc attaatttgc ctgatgattt ctaagccaaa agtaatttta
     3001 ctagatgagc cactgactgc agttgatgtt gtatcaagta ttgcaataaa acgccttttg
     3061 ttggaattaa gtgaggatca tattattata ttatcaactc atataatggc cttagcagaa
     3121 gatctatgtg atattgtggc tgtattagac aaaggaaaac tccaaacatt agatattgat
     3181 cgtaaacatg aacaattcga agagcgtctt cttcaagtgt tgaagggaga tgaatatgac
     3241 aagtaaagca ttaatatggt ctatattaca gattgatagt gctagagctt ataatagttt
     3301 tttatattac cttcagaaaa tcccctggtt gggaagaaaa atccctaacc gatggtataa
     3361 aaggtgtagt actatttatt cgtatcattt tctttttctt aagaggtttt ggtcgttcca
     3421 taggttacgt actatattgt tatgttttat cattttttgt tagaagtggc atgaagcagt
     3481 taaatatttt accgcaagaa ggttttaccc taactttaac attgatggtg attacctctc
     3541 ttttaattgt cataaatttc tatataaaac ttatagaacc taatgatatg caacaaactc
     3601 atatgattcg ccttttcaga attgagccat cgaggtattt ccaatccgct gaacttttag
     3661 aagccagtag tgaactattt tttagaagtt tgagctttgg catatttttc catttaaatc
     3721 actttgcttt ttggcatggt ttgacttttg ctttattttt agcaggtagt cgccttggaa
     3781 tcaaggtgct ctgtttacct ttatatggta gaggtaaaga ccaagagaat ccagagtctt
     3841 tactaaatat tttattttac ttagggattg gtataggagt cttcctttct ctctttttcc
     3901 ttcttcttgg caaacagttc tctccatatc catttttttc atggtacacc ggtattgcag
     3961 gacttatcat atggttagtt agttactatc gattaaaaac ttattctaaa cttaaccact
     4021 tgacctacct taccctaacg catgagacga tgaaggagaa aattgcaaag attgataata
     4081 tagagctgct tgggattgaa atgaaagata gggatataaa tgtcagtgaa ctttctcccc
     4141 tgctatttga aaatttatca ggtatttcat atttaaacgc tatcttttta aaacggatga
     4201 gtagatactt gcaaaggaaa attttggtac gtttagctgt tttaagtgtt ctgtttggaa
     4261 tagaactcct tttcctgcta ttttttaaag aaaagataaa tattccgatg aatgagcata
     4321 gtttttcaaa attccta
//
LOCUS       DATROY010000136         4287 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.137, whole genome shotgun sequence.
ACCESSION   DATROY010000136 DATROY010000000
VERSION     DATROY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            187..1395
                     /locus_tag="VBD11_001761"
     CDS             187..1395
                     /locus_tag="VBD11_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6954681.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYSNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            1589..2084
                     /locus_tag="VBD11_001762"
                     /pseudo
     CDS             1589..2084
                     /locus_tag="VBD11_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(2059..2314)
                     /gene="mobC"
                     /locus_tag="VBD11_001763"
                     /pseudo
     CDS             complement(2059..2314)
                     /gene="mobC"
                     /locus_tag="VBD11_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2324..2461)
                     /locus_tag="VBD11_001764"
     CDS             complement(2324..2461)
                     /locus_tag="VBD11_001764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954682.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2594..3738
                     /locus_tag="VBD11_001765"
     CDS             join(2594..2828,2828..3738)
                     /locus_tag="VBD11_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO6954683.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEENYFNAIK"
     gene            complement(3859..4191)
                     /locus_tag="VBD11_001766"
     CDS             complement(3859..4191)
                     /locus_tag="VBD11_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6954684.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
BASE COUNT         1400 a          852 c          684 g         1351 t
ORIGIN      
        1 acaaaatcac ctttatttgt catataaata gtagtataat aatggtgaga gatgatgaag
       61 agagagtttg ctaattagcg tgtcttttta gtgtatccac aagatagttg ttttcatcat
      121 tttattttgt agtctttaca aacctaaaga aaaattctag aaatgataat gaaggcggtc
      181 tatcaaatgg ttacattcga ttttaaacat gttcaaacat tgcataccat atctcaacta
      241 cctatttcag tcatgtcacg agacaaggaa ctcgttcagt tatatagtaa cgaagactat
      301 ctgttgcctt actatcagtt tttaaaacat ttagctatcc cttataacca agatatcact
      361 gtttatgagg gtctttttga agaatcattc ttaatttttc ctgtctgtca atatcttatt
      421 gctattggac cattttatcc ctatagccct gatatcaata ggcaagaaca gttatccagt
      481 cgctttctag aacaattttc tcatcgtagt aaaaaagaaa tcttatccta tataaagctt
      541 gtcccttgtt ttcctactac cagtatacgt agtcttcttg tgtctatcga tgcctttttc
      601 caaacacagt ttgaggctag ttgccaacaa gtcatcaatc atttattaga agagtcagaa
      661 cagattgttg cggatcctga tattgttctt cacctaaaac atactaagaa aaactctttt
      721 cagttaccca ctgttttaaa ccatctcaat cacattattg atctcgttaa gctgggtaac
      781 actcaactgt taaagcaaga aattaatcgc ctcccatcat ccagtgttac ctcatcttca
      841 atccctgccc taagagctga aaagaactta actgttgttt acttaacaaa attactagaa
      901 ttaagtttcg aggaaaatac tgatgtggct aaaagttatg cgctggtaaa gcactatatg
      961 gctttaaacg aagaggctcc tgatcttatt gatgtttcaa gaattcgctg tgcagctctt
     1021 attgattttt cagaatcctt aaccaataag agcatctctg acaagcagca aatgtacaat
     1081 agtattctcc attatgtgga caaccacctc tactccaaac tcaaagtatc tgatattgcc
     1141 aactacctat atatctcaga ttcccactta cgctcagttt ttaaaaaata ctctgacatt
     1201 tccttgcaaa gttatattct aaaggcaaaa attaaggagg gacaattact actgcaaaga
     1261 ggggtaccga ttggggaagt agcgaaatta ttacattttt acgacaccac acattttctt
     1321 aaaaccttta aaaaatacgt gggaatatct tcaaacgaat atcttactaa atatcgtgag
     1381 acctcatgcc agtaacatag caatccatga aggtacggaa ttatttcatc gtccttttgc
     1441 cttcatctga aacgactaaa aacagccttc tcgtttaaac ggaagactct tttgttacct
     1501 ttcaaaatcc actggcacta ctttttagtc taaaattttc agctaatcca gtccatacaa
     1561 actctctctt tgtgctataa cataggttat ggataaaatt attaaatcaa tttctgcctc
     1621 tggtgccttc aggtcttatg tcctcgatag caccgagact gttaaattgg ctcaggaaaa
     1681 acatcatacc ttgtcgtctt caacagttgc tcttggtcgc accctgattg ctaatcaaat
     1741 attagcggct aatcaaaaag gcgatagcaa gattacggtt aaagtcatcg gtgattcttc
     1801 ctttggtcat attatctctg ttgcagatac caaagggcat gttaaggggt acatccaaaa
     1861 tactggtgtt gacatcaaaa aacggccaca ggtgaagttt tggttggctc ttttatgggg
     1921 aatggtcatt ttgtgaccat catcgactat ggcacaggta atccctatac ctcaacaacg
     1981 cctcttatta ctgggaaaat tggtgaggat tttgcttatt acttaaccga atcagagcaa
     2041 ccccccctgc tgttgggctt aacgtgcttc tcgatgaaag tgatactgag cctgagaaat
     2101 tagatggctt tggttgatag cacgcgcaat ttgattgatg ttatttccaa tccgtgttaa
     2161 tcgtgacaaa tgacatattg ggattaagaa gaactcttct agcatagact gagaaatttt
     2221 gacaattgct cttagcaatc cgctcattta attgattcaa ttctgaatct gttagaaaga
     2281 cttttttgag attggtacga taacgataaa caattgcagc ctcctaaccc atatactttt
     2341 cacgaaactc acgactgagt ggctgtgttt gattgacttc cacaatcaat tcttgaacgc
     2401 aggtcaataa gattgacacg tgttcatgag tcacttgatg attctctctt gcgataatca
     2461 aaacttcctg cacatctcaa ctaatctgct caaacttttg gtattgccaa agggtggatg
     2521 tatactataa aagtagacaa aactttgtgt gttacaatct gttttaaaag acacaaaaac
     2581 gaaaggacaa cctatgtcaa catttaaacg ctatgatgaa gaattcaaac aatctcttgt
     2641 taacctttat caaactggaa aaactcagtc tgaactctgt aaagactata gggtatccac
     2701 ttctgcgctt gcaaaatgga tcaaacagta ctctcaattc aaactcgaag ataattctgt
     2761 acttactgcc aagcaaatac aagagttaca aaaacgtaat gcgcagcttg aagaggagaa
     2821 ccttatccta aaaaaagcaa gtgccatatt catgcaaaac ttaaagtaag actcctcgct
     2881 gtctatcggt tacgctttga acacgccaca acaaccttgt gtcgtgtttt acgtgtcaat
     2941 cgctccactt actataaatt tctaaaacat aagccctcaa aaagagaatt ggataatcaa
     3001 atttataaaa aacaaatact tgagatttat accaaagcaa acaaaagact tggtgtgaag
     3061 tctatcaaag tcattcttca aagagactac gacacaaaaa tctctgaagg aagaatttac
     3121 catctgatga agaatatggc gctccctaaa atggctaccg ttaagccaaa aacagctctt
     3181 aaaaagactc aaaaaacgta tcctcaaaac ttactcaacc agaaatttta tcctgacaaa
     3241 cctaatcaag tatggtctac tgacttcacc tatatttcta ttggatataa gaaatatgtc
     3301 tatctctgcg caatacttga tctctattct agaaaatgta ttgcttggaa attgagtcac
     3361 cgtatggatg caaagttagc atgtaacact ctagaattag ctcttaataa aagaaagatt
     3421 gaaggaacac ttctctttca ttccgaccaa gggtcacaat ttaaggccag ggaatttaga
     3481 aaaataattg atgacaacaa tatcatgcat tctttttcta aacctggata tccttatgat
     3541 aatgccgtaa ctgaagcatt tttcaagtat ttaaagcata gacaaatcaa ccgaaaacat
     3601 tatcaaaata tcaaacaggt tcaattagac tgctttgaat acattgagaa tttttataac
     3661 aattacaacc cacatacggc taatctagga ctaaccccta atcagaaaga agaaaattat
     3721 tttaacgcaa taaaataaca cggttttctg tctacttatt tgacattagt ccactctgtg
     3781 aattcagatg tggaagaaga agtaactcgc aatgttttat ttgcttcttt ttcgtgtgtc
     3841 actggaaccg ctgcatcttc agcattctaa tcctacagca tgctccgctg attctgtagg
     3901 attagaatga gatgcatctg ttgataacac tgctgaagtt gacgtatctg tcgcaagact
     3961 tgttacatct gaagtaggct gtgtaacagt aacttccggt tttacaccag ctgtttgtgg
     4021 tacctcagta ctcataatgc ttgactcatc cgccaaaaca gaatgactac tcattcctag
     4081 aaatgtcaat ccaattaata ccgatgctac accaaatttg aatttcttaa tcgaaaaacg
     4141 ctgtttcgta tgtgcgacat tataatttct ttgcttatac ttcctaagca taacttctct
     4201 ccttttcaaa agtaaaaata gattaaccaa taaagttatc tatttttact ttttttaaag
     4261 ttttactgtt ttacattatc tcgataa
//
LOCUS       DATROY010000137         4258 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.138, whole genome shotgun sequence.
ACCESSION   DATROY010000137 DATROY010000000
VERSION     DATROY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..214
                     /locus_tag="VBD11_001767"
     CDS             <1..214
                     /locus_tag="VBD11_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6954685.1"
                     /translation="HVRFKEGAPNLLLKKIESNWQLNLFEGDIYLNNHLQKVVQQLPL
                     SLGDEISFQGTIVKLFPDEIQIWGGD"
     gene            186..4136
                     /gene="essC"
                     /locus_tag="VBD11_001768"
     CDS             186..4136
                     /gene="essC"
                     /locus_tag="VBD11_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEO6954686.1"
                     /translation="MKFKSGGGTDYETSLTKKVMSAYQFYEGYPDFHRSPRIIYRSSE
                     DKITVNAPGNKPNKSKDELLKLIVPPLVMIGVSILISIFRPRGIYIIATMSMALVTMI
                     FSITGYFKNRKQYKQDLQERIDSYHDYLSDKSIELQKLAKEQKRGQHYHYPTIEGLQE
                     MADTYHHRIYEKTPLHFDFLYYRLGLGEVPTSYNIHYSQPERSGKKDPLENEGYNLYF
                     NNRYIKNMPIVANLSHGPVGYIGPRGLVLEQLQLMVNQLAFFHSYHDVQFITIVPEEE
                     MDKWSWMRWLPHATLQDVNVRGFVYNQRSRDQVLNSLNQILKLRRTQREDKSAKEGTL
                     FSPHYVVIVTDEKLILDHVIMEFFTEDPTELGCSLIFVQDVMSSLSENIKTIINIKDR
                     NTGQLVIEEGELKETDFELDHFLEDYDKENISRRLAPLNHLQNLKSSIPEAVTFMEMY
                     QAEEFEDLHVQERWISHAPYKSLAVPLGLRGQDDIVYLNLHEKAHGPHGLVAGTTGSG
                     KSEIIQSYILSLAVNFHPHDVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRA
                     LVSINAELKRRQRLFAKADVNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKSNQPE
                     FMKELVSTARIGRSLGIHLILATQKPSGVVDDQIWSNSRFKLALKVADRGDSMEMLHT
                     PDAAEITQAGRAYLQVGNNEVYELFQSAWSGADYQPEKDDQGIEDHTIYSINDLGQYE
                     ILNDDLSGLDQAENIKEVPTELDAIVENIQTLTKEMGISDLPQPWLPPLSNQIAVTDL
                     RKEESVDLWSKAPSYKVVLGFMDIPSQQAQEVAYHDFEDDGHLSIFAGPSMGKSTALQ
                     TVTLDLARHNSPEFLNLYLFDFGTNGLLPLRRLPHVADFFTIDDDEKIAKFISRIKTE
                     MSDRKKALSRYNVATAKLYRQVSGETMPQILIVIDSYEGLREAQTLTNLEACFQNISR
                     DGSSLGISLVISAGRMAALRSSLMANLKERIALKLTDDSESRTLVGRHQHIMEDIPGR
                     GLIKRDDIEVLQVALPTEGTETFDIINNIQNESDAMNSKWTGPRPKAIPIVPETLTFE
                     EFKAYEDVKKASETNQISLGISLKEVDNCSIPLSKFKQLLFTSDNPDNLSNLTRFLVK
                     SVSLIKDSKVYLLDTASDYDDLTEQQIEYINNRSEIAGHLEAIVSEIYLREESGKIEP
                     WFVIIPDMNSFVAETNMDKEQILFLLDSAHKYHIYFLFGGLASYLMTNISDVPKAIRT
                     QAQYVLLGMRVMDQSVLPKTYNSKEPYLKPDMIYIHDRRQETMLKITQEIEMEH"
BASE COUNT         1401 a          809 c          792 g         1256 t
ORIGIN      
        1 tcatgtccgt tttaaagaag gagcccctaa tttattactg aaaaaaatag aaagcaattg
       61 gcagcttaat ttatttgaag gtgatattta tctgaataat catcttcaaa aagtagtgca
      121 gcagttacct ttaagtttag gtgatgagat tagcttccaa ggaacaattg ttaagctttt
      181 cccagatgaa attcaaatct ggggggggga ctgattatga aacatcatta actaagaaag
      241 ttatgtcagc ttatcaattt tacgaaggct atcctgattt tcaccgctca cctcgtatta
      301 tttatcgttc cagtgaagat aaaattacag ttaatgcgcc tggtaataaa cctaataagt
      361 ctaaagatga actgttaaaa ttaattgttc ctcctctagt gatgattgga gtcagtattc
      421 ttatttctat ttttcgtcct cgtggtatct atatcattgc aacaatgtca atggctctag
      481 tgacgatgat tttctctatc actggttact ttaaaaatcg taaacagtat aagcaggact
      541 tacaagaacg cattgattcc tatcatgact atttatctga taagtcaatt gaacttcaga
      601 agttagctaa agagcaaaag cgtgggcaac actatcatta cccaacgatt gaaggtttac
      661 aggaaatggc tgatacttat caccatcgta tttatgaaaa gacaccactt cattttgact
      721 tcttgtatta tcgcctaggt ttgggagaag taccaacgag ctataatatt cattattctc
      781 aaccagaacg ttctgggaaa aaagatcctt tagagaacga aggctataac ctctacttta
      841 acaatcgtta tattaaaaat atgccgattg ttgcgaattt atcacatgga ccagttggct
      901 atataggacc acgaggttta gtattggagc aactccaatt gatggttaat caattagctt
      961 ttttccattc ttaccatgat gttcaattta ttactattgt tccagaggaa gagatggata
     1021 agtggtcttg gatgcgttgg ttgccacatg caaccttaca agatgtcaat gttcgtggct
     1081 ttgtttataa tcaaagaagc cgtgatcaag tgctcaacag tcttaaccaa attttaaaat
     1141 tacgtcgcac acaacgcgaa gataaatctg ccaaagaagg gactttattt tctccgcatt
     1201 atgtcgttat tgtaactgat gaaaagttaa ttttagatca cgtcatcatg gaatttttta
     1261 cggaagatcc tacagaattg ggatgttctc tcatctttgt tcaagatgtt atgagttcac
     1321 tttcagaaaa tatcaaaacc attattaata ttaaagaccg taatactggt cagttagtta
     1381 tagaagaagg agagcttaaa gaaacagatt ttgaattaga tcatttctta gaggattatg
     1441 acaaggaaaa tatttcacgt cgcctagctc cgttaaatca cctacaaaat cttaaatctt
     1501 caataccaga agcagttact tttatggaaa tgtatcaagc agaagaattt gaagatttgc
     1561 atgttcaaga gcgttggata agccatgctc cgtataaaag cttagctgtt ccacttggct
     1621 tacgtggtca ggatgatatt gtttatctaa acctacatga aaaagctcat ggtcctcatg
     1681 gattagttgc aggaacaacg ggatcaggga agtcagaaat tatccagtcc tatatccttt
     1741 ctttagctgt taatttccat ccacacgatg tagctttcct tctcatagac tacaaaggtg
     1801 ggggaatggc aaatctcttt aaggacctgc cacacttact tggcaccatc accaacttag
     1861 atggtgccca atcgatgcgt gctttggttt ctattaatgc tgaattgaaa cgtcgtcaac
     1921 gtttatttgc taaagctgat gttaatcata ttaaccagta tcagaagaaa tacaaacttg
     1981 gagaagtttc agaaccaatg cctcatctct tcttaatctc cgatgagttt gccgaattga
     2041 aatccaacca acctgagttt atgaaagagc ttgtctcaac agcgcgaatt ggtcgttccc
     2101 tagggattca cttaatccta gcgacccaaa aaccatctgg cgtggtagat gaccaaatct
     2161 ggtcaaactc acgcttcaaa ctagctctta aagtagcaga ccgcggcgac tccatggaaa
     2221 tgctccatac cccagatgcc gccgagatta cacaagcagg acgtgcctac ctccaagttg
     2281 gtaataatga agtctatgaa ctcttccaat cagcatggtc aggagcagat taccaacctg
     2341 aaaaagacga ccaagggatt gaagaccata ccatttattc catcaacgac cttggccaat
     2401 atgaaatcct aaatgatgat ctttcaggct tagaccaagc cgaaaatatt aaagaagtac
     2461 ctacggaatt agatgccatc gttgaaaaca ttcaaacttt aaccaaagaa atgggcatct
     2521 cagacttacc tcaaccgtgg ctaccgcctc taagcaatca gattgcagta accgatctcc
     2581 gtaaggaaga atcagtagat ctctggagta aagccccatc ttacaaagtt gttctgggct
     2641 ttatggatat accaagccaa caagcacaag aagtagctta ccacgatttt gaagatgatg
     2701 ggcatttgtc aatttttgca ggtccaagta tggggaaatc cacagccctt caaactgtaa
     2761 cattggactt agcacgtcac aatagtccag aatttttaaa cctttatctc tttgattttg
     2821 gtaccaatgg cctcttacca ttaagacgtt tgccacatgt agcagatttc tttaccattg
     2881 atgatgacga aaaaatagcc aagtttatca gcagaatcaa aacggaaatg tctgaccgta
     2941 aaaaagccct cagtcgctac aatgttgcaa cagcaaaact ttaccgccag gtatctgggg
     3001 aaaccatgcc tcaaatcctg attgttattg acagttatga agggctacgg gaagctcaaa
     3061 cactgactaa cttagaagct tgtttccaaa atatttcgcg tgatgggtcg tcacttggca
     3121 tttccttagt tatctcagct ggtcgtatgg cagctttgag gtcgtcctta atggcaaacc
     3181 ttaaagaacg tatcgctctt aaattaacag atgactctga atcacggaca cttgtcggtc
     3241 gtcaccaaca catcatggaa gatattccag gacgcggatt aatcaaacgt gatgacattg
     3301 aagtgttaca agtggctctc ccaacagaag gcactgaaac ctttgacatc atcaataaca
     3361 tccaaaacga atctgacgcc atgaacagca aatggactgg accaagacca aaagcaatac
     3421 caattgttcc agaaacactt acctttgagg agtttaaggc atatgaggat gttaaaaaag
     3481 caagtgagac taatcagatt tcacttggta ttagcttaaa agaagttgat aattgtagca
     3541 ttccactttc gaaattcaaa cagttacttt ttacttctga caatccagat aatttgtcta
     3601 acttgacgcg cttcttagtt aaatctgttt cattgattaa agatagtaag gtttatcttt
     3661 tagacactgc ttctgattat gatgatttga cagaacaaca aattgaatat atcaataatc
     3721 gaagtgaaat agcaggacat ttggaagcta ttgtttcaga aatctattta agagaagaat
     3781 ctggtaaaat agaaccatgg tttgttatta ttcctgatat gaatagtttt gtggcagaaa
     3841 caaatatgga taaagaacag attttattct tacttgactc ggctcacaaa tatcatattt
     3901 atttcttatt tggtggctta gcatcatacc ttatgactaa tatctcagat gttccaaaag
     3961 ctattagaac acaggcacag tatgttctct tgggaatgag agttatggat cagtcggtat
     4021 taccgaaaac atataattca aaagaacctt atttgaaacc agatatgatt tatattcatg
     4081 atagaagaca agagacaatg cttaaaatca ctcaagaaat agaaatggag cattgagtat
     4141 atgaaaaaac aaagactatt actgcttttt ggaggcttat taataatgat aatgatgaca
     4201 gcatgtaagg attcaaaaat cccagaaaac cgcacgaaaa aggaatacca ggcagaac
//
LOCUS       DATROY010000138         4256 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.139, whole genome shotgun sequence.
ACCESSION   DATROY010000138 DATROY010000000
VERSION     DATROY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1066)
                     /locus_tag="VBD11_001769"
     CDS             complement(<1..1066)
                     /locus_tag="VBD11_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6954687.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEID"
     gene            1202..2752
                     /locus_tag="VBD11_001770"
     CDS             1202..2752
                     /locus_tag="VBD11_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6954688.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGIRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            2762..3502
                     /locus_tag="VBD11_001771"
     CDS             2762..3502
                     /locus_tag="VBD11_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6954689.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            3844..4074
                     /locus_tag="VBD11_001772"
     CDS             3844..4074
                     /locus_tag="VBD11_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954690.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
BASE COUNT         1434 a          789 c          682 g         1351 t
ORIGIN      
        1 aatctatttc tgcatcagaa gctctatcta agttagctag agataacaag ttatcatcat
       61 aattcaatac ttttctatca atcaagtcta atagaattga ctgtatgagt tgttcttgac
      121 tgataaggtg agttttcttc tcctcatctt gaaaattttt aaagctcata ctataaatgc
      181 tttgagttaa ctctaatggt gaaaggtcct caggtgcttc atataaatga tgatccttag
      241 ggaattgccc gtatttatta actttttttc taaacaggaa caccctaatt gagatgaata
      301 gtgtcacaat aataaagaat gaaggtaata atatccttaa caagaaactt aggataaaac
      361 catgacgctc tattatcttc tcttgatgtt caattttttt cttgtaatta tttttactgt
      421 ttgtaggtag attaaaataa gatctatccc aataaccatg aaattctaat cgtttgttta
      481 cattaaaagc tgtcaaatgg taacgattat tattttgtct tattttagga ggagttttaa
      541 gataacccaa gtgcccccaa agacgagaaa gagcaacctt tttgtcagtt gatatccaaa
      601 aatctacttt ctctaacgtt ttatcccagt cgctaatagg aatccagtta agttcaccaa
      661 catccttatg cataaataga acatttttta gtttccactg aacattgact ttaacagtat
      721 cacctgcttc accaccatta aacactttca aacggtagcc atcctccaaa tcttctatct
      781 ggtaactaac tttccttact ttaccattaa ttgaaacttc aacctgtggc ttattattaa
      841 tatcaaaatt gtcaggtaac ttacccgccg tacctaacgt gacatactgt ccattatagg
      901 acgaatcaaa ttggtaagta accttctctg taaaatcagc actattatca ttgtgaatag
      961 ttagattacc ctcataatga ggaatgttat agtccacctc atctgcttga actgaaacca
     1021 taaaaaaact aagagctaaa caaatagcca aaaaacattt cttcattatc atcctccctc
     1081 ctttgtttgt ataccatttt aacataaaaa tacaattaat cgctttcaat aagataatga
     1141 tattgaaatc gaccttgttt ttcggtaaaa taggaggaag taacttatcg gaggctgttg
     1201 aatgaaaaaa atattactaa gtttattcac tgctttgctt atcacctttg gaggtatgac
     1261 aagtatacaa gcagatgaat atctacgtgt cggtatggag gcagcctatg cgcctttcaa
     1321 ctggacacaa aatgataaca caaatggtgc agttcctatt gaaggaactg atcaatatgc
     1381 taatggttat gatgtacaag tcgctaaaaa gttagctaaa aagttaaata aaaaagttgt
     1441 tgttgtcaaa acaaaatggg aaggccttgt tccagcacta acttctggaa aactcgatat
     1501 gattattgca ggaatgagtc ctaccgaaga acgtaaaaaa gaaattaatt tttcaaagcc
     1561 ttactacatt tcagaaccta ctctagttgt taacgctgaa ggaaaatata ctaatgctaa
     1621 aaatattagt gatttcaaaa atgctaaagt aactgcccaa caaggagtct acttatacaa
     1681 cctcattgat caaatcaatg gtgtcaaaaa agaagttgcc atgggggact ttaatcaatt
     1741 aagacaagct gtagaatctg gtgttgtaga cgcctatgtt tcagaacgtc cagatgcaac
     1801 ttccgctcaa actgctaatc ctaaattaaa aatgattgaa ctgcaccaag ggtttaaaac
     1861 atctgatgcc gatacaaata tttccgttgg gatccgtaaa ggtgacaatc gtattaatca
     1921 agtcaatcaa gttctcgaaa gcatttctag agataaacaa atcgccttga tggataagat
     1981 gattaaagaa caaccatccg ttaaaaaaga aaaaaatgga aaaccaaact tctttgaaca
     2041 gatggcaact attttaaaaa acaatggttc tcaattcctc agaggaacag ctacaaccct
     2101 cctaatttct atggtaggaa ctatcgtcgg attatttatc ggccttctca tcggtgtttt
     2161 ccgtacagca ccaaaatctg acaataagtt aaaggctgcc ttacaaaaat tattaggttg
     2221 gttacttaac atctatattg aggtcttccg cgggacacct atgattgtac aatcaatggt
     2281 tatctactac ggaacagcac aagctttcgg agtttcgctg gaccgtacac tagcagctat
     2341 atttattgtt tcaatcaata ctggtgctta tatgagcgaa attgtacgtg gtggtatttt
     2401 ctctgttgat aaaggacaat ttgaagccgc aactgcactt ggtttcaccc atggacaaac
     2461 catgcgaaaa attgttctac cacaagttgt acgtaacata ctcccagcta ctggtaatga
     2521 gtttgttatc aatatcaaag atacctctgt actgaacgtt atctctgttg tcgaactcta
     2581 cttctctggt aatactgttg caacacaaac ctaccaatat ttccaaacat tcaccattat
     2641 tgcgattatt tacttcatct taaccttcac cgtaacacgt atcttacgtt acattgaaaa
     2701 acgttttgat tcagataatt acacaaccgg tgcgaatcaa ttacaagtct aggaggttgg
     2761 tatgacacaa gctattttag aaattaaaca tttgaaaaaa tcttacggaa gcaatgaagt
     2821 gttaaaagac atttcgcttt cggtcaacaa aggcgaagtt atttctatca ttggttcttc
     2881 tggatcagga aaatcaactt tcctacgttc tattaacctc ttagaagaac caagtggagg
     2941 agaaatctta tatcacggtc acaatgttct tgaaaaaggc tatgatttaa ataattatcg
     3001 tgaaaaactt gggatggtct ttcaatcttt taacctcttt gagaatttaa acatcttaga
     3061 gaacgctatt gttgcacaga ctactgttct aaaacgtgaa cgccaagaag ctgaaaagat
     3121 tgctaaagaa aacttaaatg ctgttggcat gactgagcaa tattggaaag ctaaaccaaa
     3181 acaattatca ggtgggcaaa agcaacgtgt cgcaatcgcc cgagcacttt ctgttaaccc
     3241 agaagctata ctttttgatg aaccaacttc agccttggat ccagaaatgg ttggtgaggt
     3301 ccttaaaaca atgcaagatc tagctaaatc tggattaaca atgataattg tgacacacga
     3361 gatggaattt gctaaagaag tatcagaccg cgttattttc atggacaaag gaatcattgc
     3421 agagcagggt actcctaagc aacttttcga aaatccaaca caagaaagaa ctaaggaatt
     3481 tctacaacgc ttcttaaaat aaaatttatc cacatttaaa tatagactat ttatacccct
     3541 acaggttttg tcattgtcat cattacaatg gaactggagg ggatttttta ttaccacatc
     3601 tttgatgaaa tctacgacta gcctcaacta tagatcaaat tagacagtcg ttattggatt
     3661 gtatactatt aaataagtta ctaaatctca ttttttaatt ttaatcgata tttaattaat
     3721 tatatatttg tttaaaatta aaaacatgtt gttttaatga gcttttgatg atataatata
     3781 gtatgcacaa aatgaactaa ccactgtgca agaatagttt attatctttg aaaggatttt
     3841 ataatgaaac ttaaaatcag tgttttaaca gcagcttcag ctgtcactct attattagct
     3901 acttcagcaa gtcttgcgaa ggcagacaca gtgtcttatc cttcacgtga tgccggattt
     3961 atctactctc gtgggtatgc gccttctgaa tcaagctatc cttcacgcga tgccgggttt
     4021 atctactctc gtggatacgc tccaaaacac ttcctaagtg cttacgataa ataaaaactg
     4081 accttattaa cttaaccgat acactttcac aagtataaat tttcattcaa ttacattaaa
     4141 aataattatt tagtttgctg aaaaacgatc actcatcgat cgttttttta tatgcacaat
     4201 gatagccatt agctactttc tcgttgggta ttttcctcat tcatctttca cttaac
//
LOCUS       DATROY010000139         4147 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.140, whole genome shotgun sequence.
ACCESSION   DATROY010000139 DATROY010000000
VERSION     DATROY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            115..378
                     /locus_tag="VBD11_001773"
     CDS             115..378
                     /locus_tag="VBD11_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6954691.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            383..2116
                     /gene="ptsP"
                     /locus_tag="VBD11_001774"
     CDS             383..2116
                     /gene="ptsP"
                     /locus_tag="VBD11_001774"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6954692.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            2266..3693
                     /locus_tag="VBD11_001775"
     CDS             2266..3693
                     /locus_tag="VBD11_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6954693.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            3833..>4147
                     /locus_tag="VBD11_001776"
     CDS             3833..>4147
                     /locus_tag="VBD11_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEO6954694.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNF"
BASE COUNT         1364 a          685 c          867 g         1231 t
ORIGIN      
        1 ctgtttgaga catttaatct tgaaaaataa aaagcaatat gttatacttt taacttgtaa
       61 gggttaccaa taggtgactc aaatatttac aaatgacaaa aggagaacca taatatggct
      121 tcaaaagatt tccacattgt tgcagaaaca ggtattcacg cacgtccagc tactttgctt
      181 gtccaaacag ctagcaaatt tgcttcagac atcacgcttg attacaaagg taaagcagta
      241 aaccttaaat caatcatggg tgttatgagt cttggtgttg gtcaaggtgc tgatgttact
      301 atttcagcag aaggtgcaga tgcagatgat gcaattgcag ctatcgaaga aacaatgaca
      361 aaagaaggat tggcataaga aaatgacaga aatgcttaaa ggaatcgcag cctctgatgg
      421 tgttgcagtt gctaaagcat atctacttgt ccaaccggat ttgtcatttg agactgtaac
      481 agtcgaagat acaaacgcag aagaagctcg ccttgatgtt gcactccaag cttcacaaga
      541 cgagctttct gttatccgtg agaaggcagt agaaagctta ggcgaagaag ccgcagctgt
      601 atttgatgct catttaatgg ttctttctga cccagaaatg attaatcaaa ttaaagagac
      661 tattcgtgca aaacaagtaa atgcagaaac aggtcttaaa gaagtgactg atatgttcat
      721 cactatcttt gaaggtatgg aagataaccc atacatgcaa gaacgtgcag cagatatccg
      781 tgatgttgcc aaacgtgtct tagcacatct tttgggtgtt aaattaccaa acccggcaac
      841 tatcaatgaa gaatcaatcg ttattgcaca cgatttgaca ccttctgata cagctcaatt
      901 aaataaacaa tttgttaaag cttttgtaac aaatattgga gggcgtacaa gtcactcagc
      961 tattatggca cgtacactcg aaatcgcagc tgttcttggt acaaatgata ttactgaacg
     1021 tgttcaagat ggtcagttga ttgctgttaa tggtattact ggtgaagtta tcattgaacc
     1081 aactgaagct caaatttcag ccttcaaggc agcaggtgaa gcttatgcca agcaaaaagc
     1141 tgaatgggca cttcttaagg atgcacaaac agttactgct gatggtaaac attttgaatt
     1201 agctgctaat atcggtacac ctaaagatgt tgagggtgtt aacgaaaatg gagcagaagc
     1261 agttggtctt taccgtacag agttcttata tatggattca caagatttcc caacagaaga
     1321 tgagcaatat gaagcatata aagcagttct tgagggtatg aatggtaaac cagttgttgt
     1381 tcgtacaatg gatatcggtg gagataaaga gcttccttac tttgatcttc caaaagagat
     1441 gaacccattc ttaggattcc gtgcattgcg tatttcaatt tctgaaactg gtgatgcaat
     1501 gttccgcaca caaatccgag cacttcttcg tgcttcagtt catggacagc tacgcatcat
     1561 gttcccaatg gttgctttgc ttaaagaatt ccgtgctgct aaagctatat ttgaagaaga
     1621 gaaagcgaac ttgttagctg acggtgtagc agtagctgaa ggcattgaag ttggtattat
     1681 gattgaaatc ccagcggcag ctatgcttgc agaccaattt gcaaaagaag ttgatttctt
     1741 ctcaattggt actaatgatc ttatccaata cacaatggct gcggaccgta tgaatgagca
     1801 agtttcatac ctctatcaac catacaatcc atcaatcctt cgtttgatta acaacgttat
     1861 caaagctgct catgctgaag gtaaatgggc aggtatgtgt ggtgagatgg ccggtgatca
     1921 aactgcggtt ccacttcttg ttggtatggg attggatgaa ttctcaatgt cagcaacctc
     1981 agtacttcgt acacgtagct taatgaaaaa attggacaca gctaaaatgg aagaatacgc
     2041 taatcgtgct ctttcagagt gttcaactat ggaagaagtt atcgaacttc aaaaagaata
     2101 cgttgacttt gactgagaat aataaaaaag tagggattaa aaaatctcta cttttttatt
     2161 gaaagtttgg atattgttaa gtttctaaga aaaatatgtc agataattga aataaataga
     2221 taaagtgata gaataaacct attacaatgt aaaaggagat ttactttgac aaaagaatat
     2281 caaaattatg tcaatggcga atggaaatca tctgttaatc agattgagat tttgtcacca
     2341 attgatgatt cttcattggg attcgtgcca gcgatgactc gagaagaagt tgatcatgct
     2401 atgaaagcgg gtcgtgaggc tttaccagct tgggctgctt taacagtata tgaacgtgca
     2461 caataccttc ataaagccgc agacattatt gaacgtgata aagaagaaat tgctactgtt
     2521 ttagcaaaag aaatttctaa agcttacaat gcttcagtaa ctgaggttgt aaggacagct
     2581 gatcttattc gttatgcagc agaagaagga attcgtttat caacttcagc tgacgaaggt
     2641 ggaaaaatgg atgcttcaac aggtcataag ttggctgtta ttcgtcgtca accagtaggt
     2701 atcgttttag caatcgcacc ttataattac cctgttaacc tctcaggatc aaaaattgcg
     2761 ccagctctaa ttggtggaaa cgttgtgatg tttaaaccac caacacaagg ttcagtctca
     2821 ggacttgttt tagcaaaagc ttttgcagaa gcaggtcttc cagcaggtgt ctttaatact
     2881 attacaggac gcggttctga gattggagat tacattgttg agcatgaaga agttaatttt
     2941 attaacttta caggatcaac gccagttgga caacgtattg gtaagttggc aggaatgcgt
     3001 ccaattatgc ttgagttggg cggtaaggat gcaggtatcg tcttagctga tgctgacctt
     3061 gataacgctg ctaaacaaat cgttgcaggt gcttatgatt actctggaca acgctgtacg
     3121 gcaattaagc gtgtgcttgt cgttgaagaa gttgcagatg aattggcaga aaaaatatct
     3181 gaaaatgtag caaaattatc agtaggtgat ccatttgata atgcaacggt gacaccggtt
     3241 attgatgata attcagctga ctttattgaa agcttagtag tagatgcacg tcaaaaaggt
     3301 gcgaaagaat tgaatgaatt taaacgtgat ggtcgtctat taactccagg attgtttgat
     3361 catgttactt tagatatgaa actagcttgg gaagagcctt ttggaccaat tctcccaatt
     3421 attcgtgtca aggatgcaga agaagctgtt gctattgcca acaaatctga ttttggatta
     3481 caatcatcag tctttacacg tgatttccaa aaagcatttg atatagcaaa taaacttgaa
     3541 gttggtacag ttcacattaa caataagact ggacgtggtc ctgataactt cccattctta
     3601 ggactcaaag gatctggtgc aggtgttcaa ggtatcagat attcaattga agcaatgaca
     3661 aatgtaaaat cgattgtttt tgatatgaaa tagatgtaaa acgactcaac gattgtcagt
     3721 tgagtcgttt tataaacctc ttttacatta aaaataaaga attatataat cttctaaagt
     3781 ggttttattt ttgtgatata attgtcataa aaaataacaa aagagtcatt atatgaagaa
     3841 attaattata ggtattttta gtctaattat tatagcgatt cttgcttggc aaggtttttc
     3901 gtttttgaaa cataaggaaa taaaacttca acaagctgtt gttgaaaaag aaattcgcat
     3961 agccgagaaa acggtagaag tggtgaaaag acagaaaact gaacgtgttc tttttcttga
     4021 acctaaagga tatgacaaaa gtctaagcgc tgatatttta aagtggaatc aaaaaagttt
     4081 tgagcataaa aaattttatg acaatcaata cattattcta cgtcctcaat tagcagattc
     4141 taacttt
//
LOCUS       DATROY010000140         4132 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.141, whole genome shotgun sequence.
ACCESSION   DATROY010000140 DATROY010000000
VERSION     DATROY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            277..1422
                     /locus_tag="VBD11_001777"
     CDS             277..1422
                     /locus_tag="VBD11_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954695.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1422..2057
                     /locus_tag="VBD11_001778"
     CDS             1422..2057
                     /locus_tag="VBD11_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954696.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            2060..2986
                     /locus_tag="VBD11_001779"
     CDS             2060..2986
                     /locus_tag="VBD11_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6954697.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2987..3637
                     /locus_tag="VBD11_001780"
     CDS             2987..3637
                     /locus_tag="VBD11_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954698.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3699..>4132)
                     /locus_tag="VBD11_001781"
     CDS             complement(3699..>4132)
                     /locus_tag="VBD11_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954699.1"
                     /translation="YATILSISAIGSIIGALIARKIKSSINSMLSMLVFSSLGVIVMG
                     FPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDEAYMGRVMSTIFTIAIMFM
                     PIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQF"
BASE COUNT         1355 a          737 c          701 g         1339 t
ORIGIN      
        1 attctagcaa acttattctg ctttgtcaac cacttttttt aagttgttaa ctacgcgcta
       61 ctagaaactg ctctctcgag acaacttatt tagtttacta catcatctct tactttgtca
      121 actctttttt catacttttt ctacattttc tgaaaatgta gatcgggcgc aagcttaacg
      181 attcttttta aaatcattaa attttaaaac aaatttcaga catattgcca aagttttgat
      241 attattacta taatatagtt tgtagaggag aataatatgg gccaagaacc tatcatcgaa
      301 tatcaaaata tcaataaagt gtatggggaa aatgttgcgg ttgaagatat taaccttaaa
      361 atttaccctg gtgatttcgt ttgtttcatc ggtacgagtg gatcaggtaa aacaacatta
      421 atgcgtatgg ttaaccatat gttaaaacca acaaatggta ctctattatt taagggaaaa
      481 gatatctcta ctattaaccc cattgaatta agacgcagaa ttggatatgt tatccaaaac
      541 attggtttaa tgcctcatat gaccatttac gaaaatatag ttcttgtacc aaaattattg
      601 aaatggtcag aagaagctaa aagagctaaa gcaagggaac ttattaaatt agttgaatta
      661 cccgaagaat atttggatcg ctaccctagt gagttgtctg gcggtcagca acaacgtatc
      721 ggtgtcattc gcgctcttgc agcagaccaa gatattattt taatggatga gccttttgga
      781 gctctggatc ctattactag agaaggtatt caagacttag tcaagtctct tcaggaagaa
      841 atggggaaaa ctatcatctt agttactcat gatatggatg aagccctcaa gttagcaaca
      901 aaaattattg ttatggacaa tggtaaaatg gtccaagaag ggacacccaa tgatctctta
      961 catcatcctg ctactagttt cgttgaacaa atgattggag aagagcgtct ccttcatgcg
     1021 caggctgata ttactcctgt taaacagata atgttaaata atcctgtttc aataactact
     1081 gaaaaaacac taactgaagc tattacacta atgcgccaaa aacgcgttga ctcacttcta
     1141 gtaaccgata acggtaaatt aattggtttt attgacttag aatctctaag cagtaaatat
     1201 aagaaagatc gacttgtttc tgatatctta aaacatactg atttttatgt tatggaagac
     1261 gacttactta gaaatactgc tgagcgtatt ttaaaacgtg gtttaaaata cgctccagtt
     1321 gttgaccatg agaataactt aaagggcatt gttactcgtg catccctagt tgatatgtta
     1381 tacgatatta tttggggcga tactgaaacg gaggatcaat aatggtcaat ttcttatcac
     1441 agtatggcat gcaaatatta gtaaaaacat gggaacaagt ctatatctca ttttttgcta
     1501 tagcacttgg gattgcaatt gctgttccat tgggtgtggt cttaacacgt tttcccaaag
     1561 tagccaaaat tatcattgct atagctagca tgctacaaac tataccaagt ttagccttat
     1621 tagctttaat gattccattg tttggaattg gaaaagtccc agctatcgtt gctttattta
     1681 tttattcctt gcttcctata ctaaggaata cttatatagg tatgaataat gtcaatccaa
     1741 cacttaaaga ttgtgctaaa gggatgggaa tgaaacctat tcaatctata tttcaagttg
     1801 agttaccttt agctactcca attattatgg ctggtattcg cttgtctact atttatgtta
     1861 ttgcttgggc tacattagct tcttatatag gtgcaggtgg acttggtgat ctcattttta
     1921 gcgggttaaa tctttttcag cccaaattaa ttctaggggg cacaattcct gttattatcc
     1981 tgtcacttat tatagattat cttttaggat tactggaaac agctctaacg cccagaacaa
     2041 caagaaggga ggcttagata tgcttaaaaa atcgcacttt ttacagatat ttacactttg
     2101 cttagccctc ttaacgattt ctggttgtca attaaccgat actaaaaaac ctggtcatac
     2161 cacaattaag gttgctgccc aaagttctac agagtctagt atcatggcaa atattgtcac
     2221 cgaattaatt catcacgaat taggatacaa cacaacttta ataagcaatc ttggttcctc
     2281 tacggttact caccaagctt tgctccgtgg tgatgctgac attgctgcca cacgttatac
     2341 aggaacagac atcacaggaa ctcttggctt aaaagctgtt aaagacccta aagaagcttc
     2401 taagattgta aaaactgaat tccaaaaacg ctacaatcaa acttggtatc ctacttatgg
     2461 tttttctgat acttatgcat tcatggttac taaagagttt gccagacaga ataaaatcac
     2521 caagatctct gatctcaaaa agttatcaac aactatgaag gcaggggttg atagttcatg
     2581 gatgaatcgc gagggagatg gatacactga tttcgctaaa acatacggtt ttgaattttc
     2641 acatatttac cctatgcaaa ttggcttagt ctatgatgcg gttgaaagta acaaaatgca
     2701 atctgtatta ggctactcca ctgacggtcg tatttcgagc tatgatttag aaattttaag
     2761 ggatgataaa aaattctttc ctccttatga agcctctatg gttgtcaaca attctatcat
     2821 caaaaaagat cctaaactaa aaaaattact ccatcgactc gatggtaaaa tcaatttaaa
     2881 aacgatgcaa aaccttaatt atatggtaga tgataaactt ttagaacctt cagttgttgc
     2941 caaacaattt ttagaaaaaa accattattt tagaggagat aaataaatga aacaaatgaa
     3001 tactttccaa caatttatct actacttcca acataatggt agttatatct tagaacaatt
     3061 tatccaccac tttttaattt caatttacgg cgttatttta gctgcaattg ttggtatccc
     3121 tctaggtatc ttcatcgcta gacgtggtaa aattaaaacc tttgttatgg gaatcgcaaa
     3181 tgtcatccaa accattcctt ctttagctat gatctctatc attatgttag gtctagggct
     3241 aggtattaaa acagtagttg caactgtctt tctttattct cttctgccaa tcatcacaaa
     3301 tacttacact ggtattagaa atgttgactc cgacttacta gatgctgcta aaggaatggg
     3361 catgactaag cgtcaacgtt tatttatggt tgagttacca ctatctattt ctgttatcat
     3421 ggctggactt agaaatgccc ttgttgtagc aattggtatt acagccattg gtgctttcgt
     3481 cggtggaggc ggacttggag atatcatcat aagaggaact aatgcaacaa atggtggtgc
     3541 tattatctta gcaggatctc ttccaacagc ccttatggca attttctctg atttaatcct
     3601 aggcggtatc caacgcatgc ttgaaccaag aaaataacta ttaaaaactt gtcagtagag
     3661 acaagttttt ttgatgtttc agaataattt cttaagttct aaaattgttt tttactatag
     3721 ctaaatccta atcctcccaa tatagcaatg gcacatccta taacaataaa gctaacattt
     3781 gataaggcaa atgaaaagat agtcataaat agtgttccta taggcataaa cataatagca
     3841 atagtaaata tagtcgacat cacacgcccc atatacgctt catcgaccct gatttgtacc
     3901 tgactgaaaa aatgaatatt aaacattgtc agaagactat taaaaagaaa actaccactg
     3961 taagatatcc atatcggtaa ttcaaataga gatgggaatc ccataactat gactcccaaa
     4021 ctactgaata ctagcataga tagcatagaa ttaatactag atttaatttt tctagcaata
     4081 agcgctccaa taattgatcc gatagctgat atactaagta ttgttgcata ag
//
LOCUS       DATROY010000141         4099 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.142, whole genome shotgun sequence.
ACCESSION   DATROY010000141 DATROY010000000
VERSION     DATROY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..446)
                     /locus_tag="VBD11_001782"
     CDS             complement(<1..446)
                     /locus_tag="VBD11_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954700.1"
                     /translation="MIKFTYSANFFYYNCSKRIKEKMDSINQLIDEYGYTSSRLAANR
                     NTARKPKISRNAITLDYTKEFSRALNNKITQRNPNFITPALTEDILNFCKPEEDFIRQ
                     LNIDGIDSCKYEFESVSEIYWGTDEELYKNEELFFYAIIKDMLNSI"
     gene            1227..1652
                     /locus_tag="VBD11_001783"
     CDS             1227..1652
                     /locus_tag="VBD11_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954701.1"
                     /translation="MKKINLNSTKLGELRFSVNEIEAPQVAIVTNARRVAKRDGNGEV
                     IDGTIAKLTILVLDIKLYQLMNQQGLDVSSVVPITVEVVGDENYLKQIIVEDIIGKVI
                     DLRSAYVGLKWVSRGNSGNWGGLKLILEELKFVNLNKDN"
     gene            1727..3133
                     /locus_tag="VBD11_001784"
     CDS             1727..3133
                     /locus_tag="VBD11_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO6954702.1"
                     /translation="MMKTIFKEIISTYSLKNKKVATIIVSRTRKQRCQLLTNILLSIL
                     SVEMMFGLLYLLFDFVNSNQISISIIFSLVGLVCLESIMVLNWLFLNSKFSPIEKWKV
                     SALLKLFPEENDILRKHKSEIDTTQSVRWEYWIDNGLIFLKLYHGGHVPKGKLEEIPT
                     NILSFFIKETRDEWFLEDKNLADGYVKVVLSHKPDKSLYIKGREDFIESKTLKIRLTE
                     RLNWTLSQTNALIVGPSNSGKTTLLKYLILAFLINNKKNEVYTIDGKSAYLSNAMRHI
                     GEVATTGQQAFDMISKVEAIMEERYAEINQNSDDEADVTHNEKFGQGNILLVIDEYLA
                     LATEMQAEDKLKKPADRLFPQFNAKLLKLIVKCRQASIMVVVSGQLLPTSILSSESRD
                     SLGLRIALGRISQSQSTEIFNIGKSELPSVDSSKREGVIYLDGIGLEHPITFKTPFYD
                     DSRLPFKGALRVLKSERS"
     gene            3235..>4099
                     /locus_tag="VBD11_001785"
     CDS             3235..>4099
                     /locus_tag="VBD11_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954703.1"
                     /translation="MTSEYNLKVINFGDHVEVIKYGYLKQRVSGNNRPTNHERQIRTE
                     EEQEIYNFQQAQKIQRRIRYYCECNDFDLFWTLTFDDEKVNAKDYEYAKKKLRAWLKY
                     QREKYGKFDYIFIPELHKNGRIHFHGITFGFSPPLIEARNPKTDKLIKKNGLQIYNAE
                     TWKDGFSTVSKIDSKAKTANYISKYITKDLIAIPSAYRQPKYFVSRGLKKPEIEFQQI
                     DENELQEFTPNFIVGNLYIEEQKFEKDVSIYLLELDDENKFFQKKKPETVVRSRLKSK
                     NDENIENVSDEN"
BASE COUNT         1497 a          565 c          751 g         1286 t
ORIGIN      
        1 tcaatggaat ttagcatatc cttgatgatt gcatagaaaa ataattcttc attcttatag
       61 agttcttcat ctgttcccca ataaatttct gaaacagatt caaattcata tttacaagag
      121 tcaataccat ctatatttag ttgtctaata aaatcctctt caggtttaca gaaatttaat
      181 atatcttctg taagtgctgg tgtgataaaa ttaggattcc tttgggtgat tttattattt
      241 aaagctcgag agaactcttt tgtatagtca agtgtaatag catttctact aatttttggt
      301 tttctagctg tatttctatt agcagctaga cgactagaag tataaccata ttcatcaatt
      361 aattggttaa ttgagtccat tttttccttt attctctttg aacaattgta ataaaagaaa
      421 ttggcactat atgtaaattt aatcataaaa agctcctaaa aaattttgta cttctattat
      481 aacctaataa tgttctttgc taaggggaga atttggactt cttattctct attttttccc
      541 cttagcaaag aatttgcttc cttgtataat gagaatagag ctgacagcca ctcccaatca
      601 aatgtttttg ggggcgaaaa tagtgaacaa aagtttgaaa actcccctta gcaaagttct
      661 ctctacttta tataataagg gtagagctgg cagtcagtcc caaccaaata ttgggggcga
      721 aaatagtgaa ctaaaagttt gaaaactccc cttagcaaag ttctctctac tttatataat
      781 aagggtagag ttggcagtca gtcccaacca aatattgggg gcgaaaatag taaactaaaa
      841 gtttgaaaac tccccttagc aaagttctct ctactctata taataagggt agagttggca
      901 gtcagtccca accaaatatt gggggcgaaa atagtaaact aaaagtttga aaattcccct
      961 tagcaaagtt ctctctactc tatataataa gggtagagtt ggcagtcagt cccaaccaaa
     1021 tattgggggc gaaaatagta aactaaaagt ttgaaaactc cccctaccaa agttctctgt
     1081 tctctatata ttaaggttaa tattaggtag ctagttctca aattaaagta acagaggaac
     1141 gaccatagaa ttttatagct ataaagtcta atcaatgctt gtcggcttag ttaagacata
     1201 aataatttat atagaggtaa ataacattga aaaaaatcaa tttaaatagt acaaaacttg
     1261 gagaacttcg tttttcagta aatgaaattg aagctcctca agtagcaata gtcaccaatg
     1321 ctagaagagt agcaaaaaga gacggcaatg gtgaagttat tgatggaaca atcgcaaagc
     1381 ttacgatttt agttttagat attaagctat atcaacttat gaatcaacaa ggattagatg
     1441 tttcaagtgt tgtacctatt acagtagaag tcgtaggtga tgaaaattat ctaaaacaga
     1501 ttattgttga agatataatt ggaaaagtta ttgatttacg ttcagcctat gtaggtttga
     1561 aatgggtttc tagaggcaat tcaggaaact ggggtggatt aaaattaatt ttagaagaat
     1621 tgaaatttgt taatttaaat aaagataact aaaaattaaa taggaatatt agggataggg
     1681 ggtagcaatg ctacatgggt atatattttt aacaaggaaa aaattaatga tgaaaacaat
     1741 atttaaagaa ataataagta cttactcact aaaaaataag aaagtagcaa ctatcatagt
     1801 gagcaggaca cgaaaacaac gctgtcagct attgactaat attttgttgt ctatcttatc
     1861 agttgagatg atgtttggct tactatacct gctctttgat tttgtgaact ctaaccaaat
     1921 ttcaatatca atcatattta gcttagtagg cttagtttgc ttggaaagta ttatggttct
     1981 gaactggtta ttcttaaact ctaagttttc tcctattgaa aaatggaagg tgtcagcctt
     2041 attaaagctc tttcctgaag aaaatgatat tttgagaaaa cataaatcag aaattgatac
     2101 aacacaaagc gttagatggg aatattggat agataatggc ttgatatttt tgaaattata
     2161 tcacggtggt catgttccaa aaggcaaatt ggaggaaatc ccaactaata ttctatcatt
     2221 tttcattaaa gaaacacgtg atgaatggtt tttagaagat aagaacctag cagatggata
     2281 tgtgaaggtt gtcttgtctc acaaaccaga taaatcactt tatataaaag ggcgtgaaga
     2341 ttttatagaa tcaaagacat taaagattcg attgactgaa agactgaatt ggacattatc
     2401 acaaactaat gctcttattg ttggaccgtc aaactctggt aaaacaacct tactgaaata
     2461 tctaattcta gcattcttga taaataataa gaaaaatgaa gtttatacga ttgatggtaa
     2521 atcagcctat ctaagtaatg cgatgcgaca cattggtgaa gtagcaacta cagggcaaca
     2581 ggcgtttgat atgatttcta aagttgaagc tattatggaa gaacgttatg ctgaaatcaa
     2641 tcaaaattct gatgatgaag cagatgtgac acataatgaa aaatttgggc aaggaaatat
     2701 tttgctagtt attgatgagt atttagctct agctacagaa atgcaagcag aggataaatt
     2761 aaagaaacct gctgatagac tgtttccaca atttaatgcc aaacttttga aattaattgt
     2821 taaatgtaga caagcttcta ttatggttgt tgtttcgggt cagctgttgc ctacgtctat
     2881 tttgagctct gagagtagag attcacttgg tttacgtatt gctttgggtc gtattagtca
     2941 atctcaatca acagaaattt ttaatatagg aaaatcagaa ttgcccagtg tggactcaag
     3001 taaacgagaa ggtgttatct acttagatgg tattggatta gagcacccaa ttactttcaa
     3061 gacaccattt tatgatgatt ctaggctacc attcaagggg gcgttacgtg ttctgaaatc
     3121 agagcggagc tagctccgct ctgattctag gaataaataa ggatactttt ggctggctac
     3181 ttgacagaca gccaagataa gctgagggta tgatagaaag ttggtaaatt atgagtgact
     3241 agtgaataca atttaaaagt tataaatttt ggagaccatg ttgaggtaat aaaatatggt
     3301 tatctaaaac aaagagtatc aggtaataat agacctacca accatgaaag acaaatacgt
     3361 acagaagagg aacaagagat atataatttt cagcaagctc aaaaaattca acgaagaatt
     3421 agatattact gtgagtgtaa cgattttgat ttattctgga cattaacttt tgatgatgaa
     3481 aaagttaatg caaaagatta tgaatatgcg aaaaagaaat taagagcttg gttgaaatat
     3541 cagcgtgaaa aatatggaaa atttgactat atttttatac cagaattaca taagaatggt
     3601 agaatccatt ttcacggaat aacttttgga ttctcaccac cattaataga agcaaggaac
     3661 ccaaaaacgg ataaattgat aaaaaagaat ggtttgcaaa tatataatgc tgaaacttgg
     3721 aaagatggtt tcagtaccgt atcaaaaatt gatagcaagg caaaaacagc aaattatata
     3781 tcgaagtata ttacaaaaga tttgattgct attccttcgg cgtatagaca gccaaaatat
     3841 tttgttagtc gtggtttgaa aaaacctgaa atagaatttc agcagattga tgaaaatgaa
     3901 cttcaagagt ttacccctaa tttcatagta ggtaacttat atattgaaga acaaaaattt
     3961 gaaaaagatg tctcaattta cctgttagaa ttagatgatg agaataaatt ttttcaaaag
     4021 aaaaaacctg aaacagtcgt aagaagtaga ttgaaaagta agaatgatga gaatattgag
     4081 aatgtttcag atgagaatg
//
LOCUS       DATROY010000142         4054 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.143, whole genome shotgun sequence.
ACCESSION   DATROY010000142 DATROY010000000
VERSION     DATROY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(357..2654)
                     /locus_tag="VBD11_001786"
     CDS             complement(357..2654)
                     /locus_tag="VBD11_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6954704.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            2765..4024
                     /locus_tag="VBD11_001787"
     CDS             2765..4024
                     /locus_tag="VBD11_001787"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6954705.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         1291 a          783 c          664 g         1316 t
ORIGIN      
        1 cacaaaaaga gcagctaaat ctcactttat caagttgatt taactgctgt atatctaggt
       61 cggacaatat ttcaacctag acagacgaca aatagttacg tacattataa cttattttca
      121 ccaataaatc aagttttatt ctcttgactt caaggaagct tctagaattt caatttgttc
      181 atcaacaccc ttgagtttta gcataacacg atgattttta aacatagcat actggtgttt
      241 ttctaattct ttgaaagaag actatacaag aattaaatca tattatcttg gaaaaaataa
      301 aaggagaaca gatcactcta ttctcctcaa ataaaactat cagtatcgta aacctgttat
      361 cgttttccac ccaaagtaga ccaagctttt tgataatcac tatcagttcc cccaatagca
      421 aaacgatagg tcatcgttcc aggtccattt tttgcccaat aacttgttgt gctttcacct
      481 ccaacagtga ttcgacgacc gtttactgtt actacacctg gctgtaaacc tgtagactta
      541 agaactttag cttttataac gctatcatca agtctaaacc tttctgactt accaaaagta
      601 ttgccatctg cattattaat agcattaacc aagtgagcca tataattagc attattattg
      661 taacctgtca atttcgctaa agaggcatta ttatcatgtc ctgcccatcc acctaaagtc
      721 actttaggtg tagaaagcat caaccaaaca tcagaagttg agttagtagt acccgttttt
      781 ccaatccaat ctaccccagc taaaccacta tttaaacctt gtaagcgatt tttaaaggtt
      841 gtagtctttc cggagttaat tggtccatgt aatagctgct gtaagatagt agctgttgct
      901 ttcgaaaaga cacggacagg tttactttcg tgattataaa tcactttccc attactatcc
      961 tctatacttt ctatcatata ctgcttatga taaacaccac catttgcaat catttgataa
     1021 aggtttgttt gctgagcaac tgacgtatca ataccaccac caagtggtaa actttctata
     1081 ccaaagtttt caattggata gtctaatttc tccatatagt tttttacatc aacgcctcga
     1141 tctcgtagca tcttgtaagt ccagaaagct ggaatgttcc aagaaatgtc caatgactct
     1201 tgaaggttaa ccatagcagt accttcttca tccgcatgca taatcttttc gccacttgaa
     1261 tatgtcgttg gataatttga aagaacacta ccacttccta acatcccttg atcaatagca
     1321 attccgtaag gtaatatcgg tttaatactt gaccctggag agcgtgccgt atcaaaagca
     1381 tggttatttt gattctcact atagttacga ccaccaataa atcctataat agcaccacta
     1441 gaattgtccg ttagtacatt tcccatttga accttgccag taccatcatc taacaaccct
     1501 ccgtattgag ctgcagcatc ctgcatggct tgataaacag atttattgat agtagtttta
     1561 atagtatacc ctccctgttg aatctcttca atagcgcggt gtctgtacgt cgctctagtc
     1621 tcatcatttt ttaaatcatg ctctgaaaca ttatcctttt taatgaggta attgtacatc
     1681 actttttgag cttcagataa tgccgagtag taaagataat catgatgatt tgttgtcgcc
     1741 actgcaggtt tgataaaatc ttttttgatg tcataatctt tgtaggattt gtactcatct
     1801 ttggttaaag ctctcgttct atacatattg tataacacat ttttttggcg ctttatacca
     1861 aaactaagat ctttatctga tttcaactgg gcatctgcag tataaggaga atatacaata
     1921 ggactttgcg gtaaacctgc aaggaaagct gcctgaggaa ttgttaaatc ttttgcggaa
     1981 actccaaaaa tcccttgtgc tgcttcttca atacctgcaa tattctgtcc tttgttattg
     2041 cgaccaaacg gtgatacatt taaataatct gataaaatcg aatccttatc catgtaacgc
     2101 tctaatgcta gagcataaat aatttctttt gatttgcgtt taaatgacgg atcatctccc
     2161 aaaatttgct gctttaataa ttgttgtgtc agcgttgaac caccgctact ctctccaaac
     2221 cccagaacag atcccgctgc agcacgtagt acggctttgg gaactacacc tttatggtca
     2281 ttgaaatttt cgtcctctgt tgcaatgata gcctttttaa tattatcaga tatagcatcc
     2341 tttgccactg gtgtacgttg taaatcagtg gcaatctctg aaatttgtga tttatcagaa
     2401 taagttaacc tagacaccct agtaagtgta ttaacttgtg tcactaaact atttttactt
     2461 ggtactttaa cagagtcaac ttgactagca agatacccta ctgcaagtcc tactccgagc
     2521 ataacaaata gtaaaataat aacatagatg aaatctgata aaagctttac aattcttaaa
     2581 aaaatagaac caaattcaag tggtgtgtaa tcacctaatt tagaactatt caacttctta
     2641 ttacctttaa acatgaataa tacctcatcc tacattatat cagatttaat ataacttgca
     2701 tatcctaaag agttttgata tagttgacat taatacacaa caagttaata attaggagaa
     2761 aaagatgaat atttttgatg aactcaaaga acgtggactc gtttttcaga caactgacga
     2821 agatgcttta cgcaaagctc ttgaagaagg ttctgtctct tactatactg gctacgatcc
     2881 tacggctgac agccttcatc ttggacattt agttgctatt ttaacctctc gtcgcttaca
     2941 acttgctggt cacaagcctt atgcacttgt gggaggcgca actggactta ttggggaccc
     3001 ttcatttaaa gatgttgagc gcagtttaca aacaaagaaa actgtagtta gttggggtaa
     3061 taaaatccgc ggtcaattat ctaattttct agagtttgaa acaggtgata acaaagctgt
     3121 attggtaaat aattatgatt ggttcagcaa tattagtttc attgatttct tacgagatgt
     3181 tggtaaatat tttactgtca attatatgat gagtaaagaa tctgttaaaa aacgtatcga
     3241 aacagggatt tcctatactg aatttgctta tcaaattatg caaggatatg acttttacga
     3301 attaaataag aactataatg tcactttgca aattggtggt tctgaccaat ggggcaacat
     3361 gactgctgga accgaactta ttagacgaaa atcaaatggt gtatctcatg ttatgactgt
     3421 tccattgata acagattcta caggaaaaaa attcggaaaa tctgaaggaa atgctgtttg
     3481 gcttgatgct gacaaaacct ctccttatga aatgtaccaa ttttggctca atgttatgga
     3541 tgctgatgcc gttcgcttcc ttaagatttt tacattccta agccttaaag aaatagaaga
     3601 tatacgtatt caattcgagg aagcacctca ccaacgctta gcacaaaaaa cactagcacg
     3661 tgaagttgta actcttgttc atggtgaaaa agcttataag gaggccgtta atatcacgga
     3721 acagctattt gctggcaata taaaaggatt gtctgttaaa gagctcaaac aaggtttacg
     3781 tggagtacca aattatcacg ttcagacgga agataacctc aatatcatag accttctagt
     3841 cacttcagga gttgtcaatt ctaaacgtca agctcgagaa gatgtctcca atggagctat
     3901 ttatatcaat ggagatcgta ttcaagattt agaatatact atctcagaaa atgataagct
     3961 tgaaaatgaa attacagtca ttcgtcgtgg aaagaaaaaa tactttgtat taaactttaa
     4021 atagacttga agtgtaatcg atactctgat aata
//
LOCUS       DATROY010000143         4046 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.144, whole genome shotgun sequence.
ACCESSION   DATROY010000143 DATROY010000000
VERSION     DATROY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            394..1098
                     /locus_tag="VBD11_001788"
     CDS             394..1098
                     /locus_tag="VBD11_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954706.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
     gene            1524..2240
                     /locus_tag="VBD11_001789"
     CDS             1524..2240
                     /locus_tag="VBD11_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6954707.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            2395..3084
                     /locus_tag="VBD11_001790"
     CDS             2395..3084
                     /locus_tag="VBD11_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6954708.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            3186..4019
                     /locus_tag="VBD11_001791"
     CDS             3186..4019
                     /locus_tag="VBD11_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6954709.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
BASE COUNT         1418 a          557 c          805 g         1266 t
ORIGIN      
        1 gtattattag cttaattgta gtttctatac gaataactat tttaattgtg ttaatactta
       61 cgactgaacg ggagttgaag aaaactttca cagaaaattg cattagaaag tagaacgata
      121 gcttctagct tgattaattg aatagaatat ttttagaaat agtaaatcac aaaggtttac
      181 tattttttag ggctttgctt gttgcaatat atatatctac tgtaatgtta tacgaatcta
      241 aaataactca gcttacttca attatagcca tgtagcatag aattttattg agtttttgat
      301 ttttgatgac atgagactag atgatcaata tccgaatatg gtcactttaa tgtgtatagc
      361 tattatatta atatatttag ggagggaagt gttgtgcgcc ttgtatggaa atttctatca
      421 gaaaagttag tatgtaagat catggattct tcgtacggtg tgttgatatt attttttatt
      481 tgcctatcaa taactgctgt aggtggatat acattggtat ataatttgtc tgggtattcc
      541 tcaatggatg cacagaaagt ttttggaatc attgtactaa caataatttt actagtattt
      601 attgtaggaa caatttttta tgcaataggt tctgcacagt tgataatgtc gattttgtct
      661 aagaaaaaga tttcgtttga tgagaaagat gcaccacttg atggtgcgac tcatgtggga
      721 gagacagttt tccaaggaaa ggaggtgtcg ttatatagta agcaggataa atttttccta
      781 tggagtagaa agtggcaaaa gggtttcagc aaatatcaat attatcacat acgaccggtt
      841 gatcaaactg aaattagtga ggtgtatcaa gtggaaagat gttgcttcta ttgtgaccat
      901 atatttacac aattttttat tagagaagga aaaaatagag agaaaatagt tgagttatgc
      961 actattgatg tacaaattgg taatgacaga gatctaggat ttaaacaagt taatagaggc
     1021 gagtttgaaa aaacggttcc ttttaaggat gtcgaaatta aagaaataaa gaaattgatt
     1081 gatttgaaac aaatataaga aaagcacctt taaaagggct tttcttatat ttttctttaa
     1141 aaagctcgtt attcgttttg tagtgtgcta caattctatt gaattaatcg aataaaagaa
     1201 gcgcagttat ggtaaccact acgctttacc gagtaagtcc aattacaacg ttcccttact
     1261 tagtctttat cgtaagatac aggataacac cgaatctgtc aaggctgggc aaaggaaaat
     1321 ttgttcagtc tttttataaa aggaactcgg taggttctcc ttaatagaaa attgacaatt
     1381 ctaaaaaatt ctactgcata ccgtgataga atttccattt taccaattgc tatcaaagtg
     1441 ctataataat agagttaaaa ttaagattgc ttgccaatat gagccagcag gttattaaaa
     1501 atgaaatcaa aggagatttt ccaatgggac gtaagtgggc caatattgtt gccaaaaaga
     1561 ctgctaaaga tggtgctaac tcaaaagtat acgctaaatt cggtgttgaa atatatgttg
     1621 ctgcaaagca aggtgaacca gaccccgagt caaactcagc tctaaaattc gttttggacc
     1681 gtgctaagca agcacaagtt ccaaagcatg ttattgataa agcgattgat aaagccaaag
     1741 gaaacacaga tgaaactttc gtagagggac gctatgaagg ttttggtcca aatggttcaa
     1801 tgattattgt ggatactttg acatcaaatg ttaaccgtac ggcagcaaat gtacgtactg
     1861 cttacggtaa gaacggtggc aatatgggag cttcaggatc ggtatcctac ttatttgata
     1921 aaaaaggtgt catcgttttt gctggtgatg atgctgacac tgtcttcgaa caattacttg
     1981 aagcggatgt agacgtagat gatgttgaag cagaagaggg aacaataaca gtttataccg
     2041 ccccaacaga tcttcataaa ggtatccaag cacttcgcga taatggtgta gaagaattcc
     2101 aagttactga acttgaaatg attcctcaat cagaagtagt attggaaggt gatgaccttg
     2161 aaacttttga aaagcttatt gacgcactcg aatcagatga tgatgttcaa aaagtttatc
     2221 ataatgtagc agatttctaa aaaataaaac cgtagtatta atactatggt ttttattttt
     2281 gtgagagttg ataggtattg ttaaagaagt gataggtaaa aaatattatt caaaaagttt
     2341 ttttaggtgt aaaatgataa ttattagaca gttattggtt gatgagggag caaaatgtta
     2401 acaaagccta ttattggtat tacaggaaat gaaagagaaa tgtcagatat tccaggttat
     2461 tattatgatt cggtatctag gcatatttcg gaaggagtta aaaacgcagg cgggttacca
     2521 gttatcctac caataagtga agcagaaagt gccaaagctt atgttgagat gatagataag
     2581 ctgatcatat ctggtggcca aaatgtttta ccatcctatt atggtgaaga gaagataatt
     2641 gagagtgatg attattcact tgcgcgtgat atttttgaat ttgcattagt cgaagaagcg
     2701 ttgaaacaaa ataaaccaat ctttgcgatt tgccgtggca tgcagttagt aaatgtggcg
     2761 cttggtggta cacttaatca atctattgat aatcactatc aagagcctta cattggattt
     2821 gctcattatt taaatgtgga aaaagggagt tttctagaag gctttatatc aggtgatttt
     2881 aagatcaatt ctcttcatag acaaagtgtt aaactgttag ctgaaggtct gatagttagt
     2941 gctagagatc ctagagatgg gacagtagaa gcttatgaaa gtcgaacaga acaatgtatt
     3001 ataggtgttc aatggcatcc agaattaatg cttcaccaaa ttgaaaacca aaccttattt
     3061 ggttattttg ttaatgaaac gtagcatcca tataatagca aaaactctca gacgatagaa
     3121 gataagtatt agttaaaaac ttaaagatgt tatgatgtcg tgtacaatta aagaggagaa
     3181 aaaagatgac aataaaaaaa gtgttaagtg taacaggaat tattttagtg acagtagcgt
     3241 ctctagctgc ttgtagctca aaatctcata ctactaagac gggcaaaaaa gaagttaatt
     3301 ttgcaactgt tggaacaacg gcaccttttt cttatgtgaa ggatgggaaa ctgactggct
     3361 ttgatattga agtagccaaa gctgttttta aaggttcaga taactataaa gtcactttta
     3421 aaaaaacaga atggtcatcg gtatttaccg gcattgattc aggaaagttt caaatgggtg
     3481 gaaataatat ttcttattca tcagagagat ctcaaaaata tttattttca tacccaatag
     3541 gctctactcc ttcagtttta gcagttccta agaatagtaa tatcaaagct tataatgata
     3601 ttagtggtca taaaacacag gttgtccaag gaacgacaac tgccaagcaa ttagaaaatt
     3661 tcaataaaga gcatcagaaa aatcctgtta ctctaaaata tactaatgaa aatattacac
     3721 agattctaac gaatttgagt gatggaaaag ctgattttaa actttttgac ggaccaactg
     3781 ttaacgctat cataaagaat caaggtctca cgaatttaaa aaccatccct ttgactatga
     3841 gagatcagcc ttatatttat ttcatctttg gtcaggatca aaaagacttg caaaagtatg
     3901 tgaataatcg ccttaaacaa ttacgtaaag atgggacact ttcaaaaata gctaaagaat
     3961 accttggagg tgattacgta ccaaatgaga aagatctcgt tactcctaag gagaagtagt
     4021 aataaaaact aaggagatgt caatcc
//
LOCUS       DATROY010000144         4021 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.145, whole genome shotgun sequence.
ACCESSION   DATROY010000144 DATROY010000000
VERSION     DATROY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            110..544
                     /locus_tag="VBD11_001792"
     CDS             110..544
                     /locus_tag="VBD11_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6954710.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            547..1743
                     /locus_tag="VBD11_001793"
     CDS             547..1743
                     /locus_tag="VBD11_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO6954711.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            1798..2292
                     /locus_tag="VBD11_001794"
     CDS             1798..2292
                     /locus_tag="VBD11_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO6954712.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            2328..3194
                     /locus_tag="VBD11_001795"
     CDS             2328..3194
                     /locus_tag="VBD11_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6954713.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            3181..3996
                     /locus_tag="VBD11_001796"
     CDS             3181..3996
                     /locus_tag="VBD11_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6954714.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
BASE COUNT         1291 a          646 c          818 g         1266 t
ORIGIN      
        1 aaaaatcaag taaactgctc aaaaaatata aaaaagatgt tgacacctaa aaatgaaagc
       61 gctataatct gattataaaa gtaaaccggt aaactcaaga ggagaaaata tgataaaaat
      121 tattattgta gcacacggca actttccaga tggtatttta agctctttag aacttattgc
      181 tgggcatcag gaatatgttg ttggtattaa ctttattgca ggcatgtctt ctaatgatgt
      241 tagagttgct ttgcaaagag aggttatcga tttcaaagaa attttagtat tgactgattt
      301 gctaggtggg actcctttca atgtttcttc cgccttatca gtagaatata cagataaaaa
      361 gataaaagtt ttatcgggct tgaacctttc aatgttaatg gaagctgttt tatctagaac
      421 gatgtttgaa catgtagatg accttgttga taaagttata acatcatctc atgaaggaat
      481 tgttgatttt tctacttgcc ttgctacaca aactgcagaa gcaacttttg agggaggcat
      541 ttaataatga tgaaaataaa accggtcaag gttgagtcaa ttgaaaatcc aaagcgtttt
      601 ttagacagta gattattaac taagattgaa gttgaggaag ccattgaaaa agccttgaag
      661 caactttata ttaatattga ttactttggt gaagagtatc caacgcctgc aacattcaat
      721 aatacttata aagttatgga taacacagaa tggacaaatg gtttttggac agggtgcttg
      781 tggttagctt atgagtataa tcaggataaa aagttaaaaa acatagccca caaaaatgta
      841 ttgtcatttc taaatcgtat taataatcgt atagcattag atcaccacga cttaggattt
      901 ctttacacac catcttgtac agcagaatat cgtatcaatg gtgatgctaa agctttagaa
      961 gccactataa aagctgcaga taaattgatg gagcgctatc aagaaaaagg tggatttatt
     1021 caggcttggg gagaactcgg gtataaggaa cactatcgct taattatcga ttgcttactt
     1081 aatatccaac tcttattttt tgcttatgaa cagacaggtg atgaaaagta tagacaagtt
     1141 gcggtgaatc acttctacgc ttcagctaac aatgtggtgc gtgatgattc ttctgctttt
     1201 catacttttt atttcgaccc agaaactgga gaaccgttaa aaggtgtcac acgacagggt
     1261 tatagtgatg agtcatcttg ggcaagaggg caagcatggg gcatctacgg tattccgctt
     1321 agttaccgga aaatgaaaga ttatcagcag attatccttt ttaaaggtat gacaaactat
     1381 tttctaaatc gtttacctga agacaaggta tcctattggg accttatttt tacggatggc
     1441 tcgggtcagc ctagagatac atccgcaaca gcaacagctg tgtgtgggat tcatgagatg
     1501 cttaaacatt taccagaagt agatcctgat aaagagacat acaaatatgc tatgcataca
     1561 atgcttcgta gtctgattga acagtatagt aatagtgaat ttatagcagg acgtcctctt
     1621 ctattgcacg gtgtgtattc gtggcattca ggtaaaggag tagatgaagg taatatttgg
     1681 ggagattatt attacttaga agccttaata agattctata aagactggga actttattgg
     1741 taagagtatt ttactaacaa aataaacaat aaataataga aaacaaggag gtcagaaatg
     1801 gcagcaggac caaatattgt aatgacacgt gttgatgagc gtcttattca tggacaagga
     1861 caactttggg tgaaattttt aagttgtaat actgttattg tggcaaatga tgatgtctcc
     1921 aaagatcatc ttcaacaaac gttaatgaag acggtggtgc cagaatcaat tgcattgcga
     1981 ttttttgata ttcaaaaagt tattgatatt attcacaagg ccaacccagc acagacgatt
     2041 tttataattg ttaaagattt gaaagatgtc tatagacttg tagcaggagg cgtcccaatt
     2101 aaagagatta atattggaaa tattcataat ggagaaggta aagagcaggt aagtcgctct
     2161 atcttcttag ggatgaagga taaagagata attagaaaat taaaccaaga atatcacatt
     2221 gcatttaata ctaaaacaac tccaacaggt aacgatggtg ctgtggaagt taatatttta
     2281 gattatattt agttggacaa actcactatc atataaagga gaaaaatatg gatattagta
     2341 ttcttcaggc tgtcttaatt ggactgtgga cggctttctg ttttagtggc atgttgcttg
     2401 gcctctatac aaatcgttgt atcgttctct ctctaggcgt tggtgttatt ttaggagata
     2461 ttcaaacagc tttggcagtt ggagcaatat ctgaactggc ttacatggga tttggtgtgg
     2521 gagcgggagg gacagttcct ccaaacccaa tcggtcctgg gatttttgga actttgatgg
     2581 ctattacaac agctggaact aaaggaaaaa tcacaccgga agctgctctt gcattatcaa
     2641 ccccaattgc agttggtatt caatttcttc aaacagcaac ttatactgct tttgcaggtg
     2701 cgcctgaaac agctaaaaaa gctttgcaag caggtaattt ccgaggattt aaaattgcag
     2761 caaatggtac aatttgggca ttcgctggat tgggatttgg tttaggagta ctgggtgctc
     2821 tttcaacgca aactttgaca gatttatttg ctctcattcc ccctgttttg cttaatggtc
     2881 taaccttagc tgggaaaatg cttcctgcaa ttggttttgc gatgattctc tctgtaatgg
     2941 ctaaaaaaga actcattcct tatatcctac ttggatatgt tctagctgtt tattttggtt
     3001 tgccagttct aactccaaca gcaaatggag acggagtttt aacatcagtg gctactaaca
     3061 gtgtgcttgg ggttccaact atcggggtag ctataattgc aacaatattt gctttgctcg
     3121 atattttcag aaaaccagct gcacctacta aagaaactaa gacagaagga gacaaccaag
     3181 atgactggat ctaaaaaatt agcaaaaagt gattacacta aaacagcttt acgtgccttt
     3241 tatttacaaa atggctttaa ttacagtaac tatcaaggtc ttggttatgc taatgtcata
     3301 tatccagctc tcaaaaaata ttatggagat gacaaaaaag ctttagcggg agctctagaa
     3361 gaaaatgtgg agttttacaa tacaaacctt catttcttac cttttgtaac tagtttacat
     3421 ttagcaatgc ttgataatga gcggccagaa gaagaaattc gcggtattaa gatggcgctt
     3481 atgggtcctc ttgcaggtat cggtgattct ttatcacaat tttgcttagc acccttgttc
     3541 tcaacgattg cagcctcttt agcgacagat ggtttggtga tgggaccgat tctgttcttt
     3601 gtggctatga atactatttt aacgggaatt aaattagtta ctggcatgta tggttatcgt
     3661 cttgggacca gttttattga taaattgagt gaacaaatgt cagttatctc acgggcagct
     3721 aatatcgttg gtgtaacggt catctcaagt ttagctgcaa cacaagttaa gcttacaatc
     3781 ccttacactt ttgctccaga aaaagtaact agtacagcgc aaaaaattgt tactgtccaa
     3841 ggtatgcttg ataaaattgc accagctcta cttccagcac tctacacttt ccttatgttt
     3901 taccttatta aaaataaaaa atggacaact tataagctag ttatccttac ggttattatt
     3961 ggtatccttg gtagctggtt aggtatttta gcttaataaa attttgataa ataaggatta
     4021 t
//
LOCUS       DATROY010000145         4007 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.146, whole genome shotgun sequence.
ACCESSION   DATROY010000145 DATROY010000000
VERSION     DATROY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            470..1237
                     /gene="cfb"
                     /locus_tag="VBD11_001797"
     CDS             470..1237
                     /gene="cfb"
                     /locus_tag="VBD11_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6954715.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1410..1712
                     /locus_tag="VBD11_001798"
     CDS             1410..1712
                     /locus_tag="VBD11_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6954716.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1869..2732)
                     /locus_tag="VBD11_001799"
     CDS             complement(1869..2732)
                     /locus_tag="VBD11_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6954717.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(3037..3597)
                     /locus_tag="VBD11_001800"
     CDS             complement(3037..3597)
                     /locus_tag="VBD11_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6954718.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(3594..3911)
                     /locus_tag="VBD11_001801"
     CDS             complement(3594..3911)
                     /locus_tag="VBD11_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6954719.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
BASE COUNT         1418 a          599 c          698 g         1292 t
ORIGIN      
        1 gtctaaaata cttagtctag tatgaatttc taaacagaaa gtaaaaataa tgagttttag
       61 tatggttttt gggcataatg gaacgatttt atctaaaata gtacgtttct tattaaattt
      121 atttttttag aaaaacatta ggctaaccta tttgataaaa atagtgataa gtagaaattt
      181 tctaaaatat ttagatttat ataaggtggt aaattatcgt tatggttttt tacatgaaaa
      241 tctaaattaa attatcagaa attaacaaaa ggataggcgc aagtatatta tagcaaaatg
      301 ataatattag ttttatgaaa aaaatgcagt ttatttgtaa taatgtaaaa tataggggaa
      361 aagaaagcgc ttgacgacct tttggacaag tagtaagata caaacatggg ccctgtaaat
      421 taaaaatact gcagtaaaag tgattttagt ttaaaggagg aaatttatta tgaacgttaa
      481 acatatgatg tatctatctg gaactctagt ggctggtgca ttgttatttt caccagctgt
      541 attagaagta catgctgatc aagtgacaac tccacaagtg gtaaatcatg taaatagtaa
      601 taatcaagcc cagcaaatgg ctcaaaagct tgatcaagat agcattcagt tgagaaatat
      661 caaagataat gttcagggaa cagattatga aaaaccggtt aatgaggcta ttactagcgt
      721 tgaaaaatta aagacttcat tgcgtgccaa ccctgagaca gtttatgatt tgaattctat
      781 tggtagtcgt gtagaagcct taacagatgt gattgaagca atcacttttt caactcaaca
      841 tttaacaaat aaggttagtc aagcaaatat tgatatggga tttgggataa ctaagctagt
      901 tattcgcatt ttagatccat ttgcttcagt tgattcaatt aaagctcaag ttaacgatgt
      961 aaaggcatta gaacaaaaag ttttaactta tcctgattta aaaccaactg atagagctac
     1021 catctataca aaatcaaaac ttgataagga aatctggaat acacgcttta ctagagataa
     1081 aaaagtactt aacgtcaaag aatttaaagt ttacaatact ttaaataaag caatcacaca
     1141 tgctgttgga gttcagttga atccaaatgt tacggtacaa caagttgatc aagagattgt
     1201 aacattacaa gcagcacttc aaacagcatt aaaataatat ttgtattttt cgtgtgatgc
     1261 tgtcgacttc gtgattttgt actaccatga ttgttatgat taaaagattt acgacaatag
     1321 tcataatagt agaacgatgt caccatttta aataataaag tgattagtca tttgactaaa
     1381 tttgccaagt atcaaaggaa ataaagatta tgactaaaaa gataactgtt gtagcattag
     1441 aaacattgat tgcccagcat aataatatcc atttgataga cgttcgtgaa gagcatgagt
     1501 atcgtggagg gcatattcca ggtgcgataa atcttccttt gagtcaactc agtcataagt
     1561 ttgaacagtt agataaaaat aaggaatatt atcttgtttg tcaacgaggg ggaagatcta
     1621 ttagagcatg ccaatttctc gagttgaaag gctacaaagt aatcaatgta gatggtggca
     1681 cagaagcgtg gagaggaaat ctagaagtgt aaatatgtcc tgtgtagtct accatatttg
     1741 ataataaaaa aattgacatt atctcactat taatgacctt ttagtaaaca taaagaaaag
     1801 tatgtgtttt gaaaaggagg atgattatcc ttttcaaaac catacttttt gtgtcttgtg
     1861 gcttggtctt aattaatagt tgttaggaca atgtcgtaaa tgacaaggag aataaaaggc
     1921 caactagtaa gtaataattt tgtgtgttgt ttagagttta ttgagtcagc ctctcgaacg
     1981 ttagagtgca gacttatacg acggcgagca gtattcctat gcgtaaaaag gtaatataat
     2041 ataaataaga ttgctaaaat tagcaatata ttttcaaaat aagtcccgta taactcgttt
     2101 actgttgata gctgggtgaa aatttctacg ataaagttat tgcagatatg aaggagtagg
     2161 gctaccttaa aggaataatg gtaagcgata aaacccatta tgatacccgc aatagatgta
     2221 gtcaatagct gagaaatgtc ttggtgcatt aaaccaaata ggatgccaga agtgataatg
     2281 gcaaaccatt taccatactt ggataataaa ttcaatacta ttcctcggta taaaacttct
     2341 tcaaaaaaag gacctacaac aacagaataa ctaatcataa gtatactgaa tgtggcatca
     2401 taattttgac tttccgattc cataatattg agatgattta gcacagcatc ccatattgtg
     2461 gagactatat tattagtagt atatataatg cttaacaaga ataaaatagt ggtgaaagtg
     2521 attgtatgat gagttgccgt tagatgtttt tttaaatttt taaaacccat aaaaaaacaa
     2581 ggaatgagta aggcgattaa agtacttgct atacctatcc agacatcatt ttgtaagatt
     2641 tcagcaaata gtgtagtcgc caaacttaaa acaatagcct gagctaaaag tatcagcccg
     2701 attaggttaa gtctcttttt ttcttgattc aattgattac ctccttatta ataacggtaa
     2761 cgacaaagaa atattataat gtataatgta taggtttcta agtcattagt caatagggac
     2821 gagcacttgg atacttctaa taagataaaa tacatagaca agactatggt tttggcttct
     2881 ctaaagatta cctaaaaatt tataagttct cgagcagaaa ggatgagttt ggatattgag
     2941 aggatgggct tgaccagctg aaagaaaaat gaaccaaggg taatcgcttc gaaaaaagaa
     3001 ggaaatttca attagaaact tccttttttt atcaatctag tattggcgcg tgctagggac
     3061 tttataattc gttataaaat cttcgatttc agcaatatca tctgagaata ggacattttc
     3121 tctatcttcc agactaagaa aaccctcact aaccatatga tcaaacatat tttttaaatc
     3181 attaaagtag ccattaacat tatataaaat gcaaggatta tcattttgac cgatgcgaga
     3241 ccaagagata acttcggaaa tttcttcaag ggtaccaggt ccaccaggta gagcgataaa
     3301 ggcatctcca agtagcatca tttttgcctt acgttcaggc atattattta ctatgataag
     3361 ctctgataaa ttttcatgtg caatttcacg atctcttaaa aatgtcggaa taacaccagt
     3421 agtatagcca ccatttgcaa tcacagtatc agccatgact cccattaagc caacttttcc
     3481 tccaccatac accagagcat gtttgttttg tgccatccat tgtgccaagg caactgtttt
     3541 ttcactataa acaggatttt ccccagtact tgctccacaa aaaatagtta ttctcatgtt
     3601 tcaaacctct tttctaattt agtgcgatgt gcgctgataa tctcttctag tgagatatta
     3661 tactgatcag caagtatgaa gatattatct aagacatctc ctagttcttc ttttatatca
     3721 tttaagtttt ccaaatcagt ttgttcggtt tcgtcaggtc tgtcacgtcc aatttcatat
     3781 ttgcgaatgg cttgagccaa ttcgccgact tcttctgaaa gaaagttact tctaataaaa
     3841 gaattgtatt gataccagtt tcttttttta tagaattcgc tcacccattt ttgataatct
     3901 gtaatatcca ttgttccctc cgtttcaagt atattgtagc aatattgctt tattaatgaa
     3961 aatttgaacg ctaattaata ggaaaagttc gtatttggta tttcttc
//
LOCUS       DATROY010000146         3955 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.147, whole genome shotgun sequence.
ACCESSION   DATROY010000146 DATROY010000000
VERSION     DATROY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(109..315)
                     /locus_tag="VBD11_001802"
     CDS             complement(109..315)
                     /locus_tag="VBD11_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954720.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(466..1659)
                     /locus_tag="VBD11_001803"
     CDS             complement(466..1659)
                     /locus_tag="VBD11_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6954721.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(1691..2665)
                     /locus_tag="VBD11_001804"
     CDS             complement(1691..2665)
                     /locus_tag="VBD11_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6954722.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(2780..3003)
                     /locus_tag="VBD11_001805"
                     /pseudo
     CDS             complement(2780..3003)
                     /locus_tag="VBD11_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2981..3373)
                     /locus_tag="VBD11_001806"
     CDS             complement(2981..3373)
                     /locus_tag="VBD11_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6954723.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(3362..3437)
                     /locus_tag="VBD11_001807"
                     /pseudo
     CDS             complement(3362..3437)
                     /locus_tag="VBD11_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            complement(3434..3910)
                     /locus_tag="VBD11_001808"
     CDS             complement(3434..3910)
                     /locus_tag="VBD11_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001894769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6954724.1"
                     /translation="MSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQ
                     IVKVMQNQKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLG
                     TELDIYADEKWEDFFTKLARATQLIQPVIFIFVALIIVMIYAAMLLPMYQNMEILS"
BASE COUNT         1236 a          759 c          578 g         1382 t
ORIGIN      
        1 tgctggtaaa cgattggtgg acaaaaatca atagtccaca tccttgacat attacagatt
       61 aacttttaac cttttgtctt ttgtggactg tatttttcca ttaaaattct atcctccttc
      121 actttctgta taaataaaaa cttcctcaat ttgtttatca aaataacgcg ctatcttaaa
      181 tgctagctcc aaagatgccg tgtatttctc tttttctaga gaaataatag tttgcctagt
      241 aactccaagt gcaactgcaa gttctgcttg acttaatttc ctagattttc gtaacttttg
      301 tagagaattt ttcatatctt acaccttttt gtatagttag ctttacattt atagtatagt
      361 taactataca ttttttgtca agtacatttt acatacctgt tcaaaaaaca aaaaagttga
      421 tagttaaact atcaactttt ctggtcttat caattttttg ataacttatt tagctttaag
      481 gcgctcaacg tcacgcgcaa tcattaattc ttcatcagta ggaataacta aaaccttcac
      541 ttttgaatct ggttttgtga tatctccaaa ataaccaaaa acattttttt ctggatcaag
      601 ttcaatgcca aaccatgata atcctgcgat aacatcttga cgcattaatg gcgcattttc
      661 tcccatacct gcagtaaaaa taatagcatc cgcgccattt aatactgcca agtattgtcc
      721 aataaatttc ttaatacggt caataaagac attataggct aacactgcat ttggatcttt
      781 actttgaaga cctgcttcaa tatctcgcat atcacttgaa gtgcctgata ctccaagcaa
      841 tcctgattgt ttgttaagca tattaacaac agcagctgca tcttccaatt ctggatcatt
      901 agctacaaga taagggataa tcgcaggatc aatgtctcct gaacgtgttc ccatcattgg
      961 tcctgcaagt ggtgtgaatc ccatcgatgt atcaatagac tgtccatgat aatttgctgt
     1021 aatagaaaca ccattaccca catgtgctgt gattaatttt aattcttcta aaggacgtcc
     1081 taattgctta gctgcttctt gtgcaacgta ttggtggctt gtcccgtgtg caccgtattt
     1141 acgtacttta taatcagtat agtacttttg tgggattgga tataagtagg tatgaggttg
     1201 cattgttgta tggaaggctg tatcaaatac acatacactc gtgatatctg gtaagatttc
     1261 tctaaacgca cgaatccctg ctgcagctgc tgggttgtgg agtggagcta aagctgataa
     1321 ttcttcaacc tgttctacaa ctttatcatc tactaaagca gactctttaa aatattcacc
     1381 accagcaacg acacgatgac caacacctgt aatttcatta aagtctttga taataccatg
     1441 ttttgttaga tcttctaaaa gaattttaac agcttgggta tgatctacga tatctaaaat
     1501 ttgttcatct tttttatcgt caaattttac agtcgaaata gaatctttca aaccaatacg
     1561 ttcgataatc cctttggcaa ctactttctc ctctggcatt tcatataatt gccatttcaa
     1621 acttgaactt ccagcattaa ttgctattgt ttttgacata atttacctct ctaaagcgtt
     1681 ttcacaatct ctattctatc atattttttg agtaatgact attttctctg ctccattttt
     1741 gaaaattttc aatgaagttt gccatattct cacgattttg caaatctgtc agcggatata
     1801 caaaggtttc tggtttttgt tctgcttgct tcttgagaac aaatatagat ttcgcatttt
     1861 gacgacttcc aaaaatagtt tctggtagag ttaaaacggc aatgacatct gcatatcctt
     1921 ttaaccactc cttcagcaaa tcactttgtg gacttgttaa aaggttttcg ggtgctaaaa
     1981 atatagcgat tccatctttt ttcaaatatt taagagattg ctccatcaat agatggtgag
     2041 cataggtgtg ctctttagaa cttgatacag catatcgttt agcaatatca tcattaggat
     2101 aatagccaac tggtaaatca ctaatgatta catcgctttc tttcaaaatt tgtggtctaa
     2161 cggcatcctc ttggataaat tgggcactag aacctataat ttcagcaatg cttgctgata
     2221 gatcaatcaa aagatcatca acttcaatgc ccatataatt taactctttc gagctgttat
     2281 tgaggagagt ctgagctaaa ttcccagttc cacttccaat ttccaagaca tccactgtct
     2341 cttgactcgt taattcttcc aaaagaaata acaagataaa accaatacta tctggcgtaa
     2401 actgatgatt agcttgtaat tgctctgttt gcgcagattt gataaaaatg aactggaaag
     2461 tgcgacgcca ttcttcttga cttaagtcaa gttgacgtaa tttttggtta ttcaccacaa
     2521 caatatctaa atcacaactt gaaccaaggt aataagagtt ctgttcaatt aaggcatcat
     2581 aaatatgagt ttttaattgg ttctcaatcg tttggatatt ttctaaaatc agctcatagg
     2641 ctgtctcaat tttttcaaaa ttcatgattt cctccagaat ttatcataac aaaaaagaca
     2701 gcatctgaaa agagaagcaa acaaaaatcc tgtttttaag caaaaccttg tcttactaat
     2761 atttcatcac tgtatatttt taatgtttat ggcttgcttt attatcatta gacttgagag
     2821 gtacttggag agtgtctatt ttacgataaa acttatggtt atagtttcct cccttatcct
     2881 atgatactgt ctttctgttg ccaatattct tgctagataa aactgtaaga cgagtgtaaa
     2941 tacagcagcc aatacaatag cttgcaaaag gatgccagct ttaagtttct tctttaaaat
     3001 catagtaaaa tgtccttttt aacccgtcct taaaataaaa aacaagtttt accatacttt
     3061 tggtctgact catttgacaa ttgtctaacc cataaaccat tggttgataa cctcgaccat
     3121 tataacctgt cttacggaaa tcatctttat tagatttgcc aaaggttaca atcttatctt
     3181 gcttacgtaa ataaagtttg ttctgtctta aatattccaa gtgagcgcct tcgaattctg
     3241 catttagttg ctgagttaat aacacccatt cagactgact ggaagaagac atcactacta
     3301 tctgttgagc caacaatttt gttagtcctt gataaactag caaagctcct gtgattgtaa
     3361 ccaatgctac caaacattct aaaatgtgta aatgctttaa ccttcttatc cttacatttt
     3421 aacaataaat ttttcatgat aatatctcca tattttgata cattggtaac agcattgctg
     3481 cataaatcat aacaatgata agagctacaa aaataaaaat aacgggttgg attaattggg
     3541 tcgctctagc taattttgta aaaaaatcct cccacttctc atcagcatat atatctaact
     3601 ctgttcctaa tttcgcctta acttgaccat attcaatcat taaactaagc tcagttaaga
     3661 aaaacggata gtctaatact ttttggtgaa atgctttacc tgatagaaaa ccttcttcca
     3721 tgtcatatcc tatttcccta aaaagtttgg atttctgatt ttgcattact ttaacaattt
     3781 ggtccaattc aacaccttga cttaataaat ttccccactc acgggcatag taagcagtta
     3841 aataaagctt aacatatgat ccaactaagg gaattgttgt taaaaaacaa gctactttaa
     3901 tgcgcgacaa tcgcttttga ataatataaa atattaaact aaaaataagt acaac
//
LOCUS       DATROY010000147         3906 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.148, whole genome shotgun sequence.
ACCESSION   DATROY010000147 DATROY010000000
VERSION     DATROY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(86..601)
                     /gene="lacB"
                     /locus_tag="VBD11_001809"
     CDS             complement(86..601)
                     /gene="lacB"
                     /locus_tag="VBD11_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6954725.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(622..1046)
                     /gene="lacA"
                     /locus_tag="VBD11_001810"
                     /pseudo
     CDS             complement(622..1046)
                     /gene="lacA"
                     /locus_tag="VBD11_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            complement(1295..3745)
                     /locus_tag="VBD11_001811"
     CDS             complement(1295..3745)
                     /locus_tag="VBD11_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO6954726.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSTEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAGEDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAMQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTQKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEIHDAYVQLSAIRFEHDKKEYILLVNANEPGKKCQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
BASE COUNT         1158 a          794 c          555 g         1399 t
ORIGIN      
        1 tacagtatcc atgttaaagt tttctaagca gtacgaaata tcaatagaag ggttcaaagt
       61 tactgttaaa atcatagata tctccttagt cgtgatattc accacgattc catttatcta
      121 agaactctgt gaaaaagtca ttctcttctt gatgggcatt atgagtctca acctcggcta
      181 tcttttcaat taatactttg ttttcttttg ttggtttata tttagctctt ataaacgcct
      241 cgatgatatc tgtcattaac agcccaccag taattttccc accaaatccg ataacgttag
      301 catttaattc ttctttagca tagatagctg atgtcaaatc acgtaccaga gctgagcgaa
      361 ttcctggaac tttattaaca gcattattaa taccaactcc agttccacaa atacatacac
      421 ctaagtcggc ttttccacta gcgacagctt ctcctacttt cttaccataa ataggatagt
      481 gagtacggat gttatcataa gttccacaat caataacctc ataccctttt gtttttaaat
      541 aatccacaac tgcaattttg tcataagtta caatatgatc acaaccgacc gcaattttca
      601 tctgctttac ctctcttacg cttagcacat tttattcaac atatcgaccc gaatttgatg
      661 acgacctgca tcgtaagtcc catcaacaaa accctctact atatgtttag caaccccgga
      721 ccaacaatct ctgaacctaa agtaatcatc ctagcgttat tatgagctct agtcatataa
      781 gctgagcgtt catctgaaac ttctgctgca atcatcccct taaccttagt ggctaccata
      841 aaaggaccag caccgtaagc atcaaccatt attcctaagt tatcttcttt ttgattcact
      901 ttagcaacga ttgctaaggt attatcaacg aaatctttgc tagtatccgt taaatcaata
      961 atttccttac ctaaacgtgt aaggtgttgt ctaatcactt ccttaagttc aacaccatgc
     1021 gcatccgccc caataataat tgtcatttct ttctccttat ttcactttta atacaattgt
     1081 atgtagttgg aagatattgt ttgttttttg tttgtaatat catcatatat cttatttttg
     1141 tttttgtcaa tgttttgttt ttgtttttgt ttgaaataaa caaaaaaact ccgtgataac
     1201 tatggcaact agaattcact actagctagc tatcacgaag tttgatactt ctcattattt
     1261 ttatttgtag gaacggttat tccgcaagcc ataatcataa ataaaattta aacaaaacat
     1321 gccaatttgc tgccattttc tcccataaag tgccttgctt ctctgtattt tgactaagaa
     1381 aactccaatt aaaaactttg taatttaaag taaaactatt ttgatgtttt tctctatgtt
     1441 cataaaggac accaaattga tcattattaa gttgttgaac agagttgtaa gcaaacgaac
     1501 catcttgaat gtgatggtga tataaccact taaaactacc atttcgatta acttgcgcta
     1561 gacgtgcata tccatcttgg cacttcttcc ctggctcatt agcattcact aataaaatat
     1621 actctttttt gtcatgctca aagcgaatag ctgatagttg gacgtaagca tcatgaattt
     1681 ccttatatcg tttaacatgg ttttgccaag tctccccgcc gtctttactt gtggctactt
     1741 ctaggttacc agttagattc ctcataaata acttaatgtc cccatttttc aattgaacgg
     1801 gtacggattc tgtattttgt tcttttttat tatccattgt taaggagtga attttttcac
     1861 cattagaaag tatacggtta tcattaactg cttttccagt atgccacgtt tttccatggt
     1921 cgtctgaata aattaggcgt gaagattgtg aacctcttag atgagatttc caattcgtag
     1981 aataggcagg aataataata cgaccagcgt gtggccccca ttttaaaact ataccttttc
     2041 caggtcctat tcccaaaaat ttcatgcctt tctgacgaag acttgctgtt atatctctag
     2101 gtgatgacca tgtcctacca tcatcatcgc tatatgacat ccacacatag cttgattttg
     2161 ctaaacgaaa tggtgacgtt ttatgttttg taaaatatat atttccaagt agctgttttc
     2221 ccttataaat atcccctaga tttgaatggt cacttttagt agtttctgtt ataacatgat
     2281 aatcagtctt tttcccttta aaattatata caataccttt gtcacggata gtaaaaacct
     2341 tcgatttctt tccattatta tataaattaa gataagactg tcctccgatt tgggtatatt
     2401 ctttttcagg tgtgttagca atactaataa cgcctcttcc ttctggaaac atatcatata
     2461 ttgaaaaaat acgtttcgtc ttggagctag tatcttgaac taatgccata tccatattaa
     2521 taggtgagcc actatagtca ccactagtaa ctagcggaac tctagggtta ttacggagat
     2581 tgacaatagt ttctcttttt ccccaagtga caccatcatc ctcactacgg cgaataacca
     2641 ttcctatgtt tccccaatca caagcttgct caattctttc atcagctccc gcaagtaaag
     2701 ttcctttttg ggttttcaat aatgagggga tacggtaacc aaacacatta tctttatttt
     2761 ttctcccaaa atctcctccc ctatatactt ctgttttagc agaaagtata gcacctttag
     2821 gtaatttgag ctgatggttt agatgacaat aatattgaaa ataagatttt atatcttcaa
     2881 aattcaaatt atcaacacag caaacaaggc agtgtgcctt agcttttaat ttttgccata
     2941 aattaccact atattttatt tctttctcta ctttctcttt gccaatagca agcatcccct
     3001 ctataacaga cgataacttt ccatcctttt gagagaatgt tttagtaaga tggtgttcct
     3061 gcatcgcatt ctttttaaag cccaacgata tctgactagc aatgtccgta ttacctatga
     3121 cattacttgt cgataaatct cccttaggaa tgatatccaa agacctcaac ttatcccttt
     3181 caaaacctct atcctctccc gctgtctttc taaattcatc gctattaagt aatgcaaccg
     3241 aagaaccctt tgattctatc aggaatctct gttgttctgc aatggaatga ctgctttcgg
     3301 gtataatata ggtagttcta ttattaggtt gagttgcatt tagttctgct aaatttgcct
     3361 gtttacgatt ttcttctgtc gctatttccg attttagagg aggtgacaat ttttcttgac
     3421 tgtcagaatc acctaagact actaccatat ctttatcttt ttgatcatct actattaaac
     3481 cgtccgatgt atcagaatct aattctaact gcctctcctc tgttgaatta gtaagcttat
     3541 ctaaagtaga gtcaaatatg ggagcagtag ttttttcatc atctattgat ataaaagagt
     3601 ttatatcaga ttttatttcc tcactataaa ttgtactagg atgcaaaagt ccacataata
     3661 agataaaacc tagcatcata tatgtactag ttatcactcc tagacggcgt atcgttatag
     3721 aaggtttccc aaaggttaat gtcatagaaa ctccaagtct taaaatattg gttgcataca
     3781 tcaactctag aaaaatggga gttcagtaat gtttccaaaa aggattgtat accaacttat
     3841 cactattttc aatatatttt tataatttta ctagtttttt attattaaaa tgagcgaaaa
     3901 ttaatt
//
LOCUS       DATROY010000148         3904 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.149, whole genome shotgun sequence.
ACCESSION   DATROY010000148 DATROY010000000
VERSION     DATROY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1387
                     /locus_tag="VBD11_001812"
     CDS             <1..1387
                     /locus_tag="VBD11_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEO6954727.1"
                     /translation="DDYQIVKGDGESFKLFSDRKVPVTGGTTQAAYRVPQNQLSVMSN
                     EGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQIKTHGEPTTLYFNGNIRPKGYDI
                     FTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNVDDTNKIYDELNKYFKTIVEEKHS
                     IVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGNDGSQLKNGVALGGPNSDGGILKDV
                     TVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDNYISNKFYNTNNRTTLSPKSEKEP
                     NTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIKVNKDKHSESLLGAKFQLQIEKDF
                     SGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYEISSPDGYIEVKTKPVVTFTIQNG
                     EVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPPGVFPKTGGIGTIVYILVGSTFMI
                     LTICSFRRKQL"
     gene            1441..2231
                     /locus_tag="VBD11_001813"
                     /pseudo
     CDS             1441..2231
                     /locus_tag="VBD11_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            2480..3085
                     /locus_tag="VBD11_001814"
     CDS             2480..3085
                     /locus_tag="VBD11_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6954728.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
BASE COUNT         1303 a          620 c          823 g         1158 t
ORIGIN      
        1 tgatgattat caaatagtaa aaggagatgg agagagtttt aaactgtttt cggatagaaa
       61 agttcctgtt actggaggaa cgacacaagc agcttatcga gtaccgcaaa atcaactctc
      121 tgtaatgagt aatgagggat atgcaattaa tagtggatat atttatctct attggagaga
      181 ttacaactgg gtctatccat ttgatcctaa gacaaagaaa gtttctgcaa cgaaacaaat
      241 caaaactcat ggtgagccaa caacattata ctttaatgga aatataagac ctaaaggtta
      301 tgacattttt actgttggga ttggtgtaaa cggagatcct ggtgcaactc ctcttgaagc
      361 tgagaaattt atgcaatcaa tatcaagtaa aacagaaaat tatactaatg ttgatgatac
      421 aaataaaatt tatgatgagc taaataaata ctttaaaaca attgttgagg aaaaacattc
      481 tattgttgat ggaaatgtga ctgatcctat gggagagatg attgaattcc aattaaaaaa
      541 tggtcaaagt tttacacatg atgattacgt tttggttgga aatgatggca gtcaattaaa
      601 aaatggtgtg gctcttggtg gaccaaacag tgatggggga attttaaaag atgttacagt
      661 gacttatgat aagacatctc aaaccatcaa aatcaatcat ttgaacttag gaagtggaca
      721 aaaagtagtt cttacctatg atgtacgttt aaaagataac tatataagta acaaatttta
      781 caatacaaat aatcgtacaa cgctaagtcc gaagagtgaa aaagaaccaa atactattcg
      841 tgatttccca attcccaaaa ttcgtgatgt tcgtgagttt ccggtactaa ccatcagtaa
      901 tcagaagaaa atgggtgagg ttgaatttat taaagttaat aaagacaaac attcagaatc
      961 gcttttggga gctaagtttc aacttcagat agaaaaagat ttttctgggt ataagcaatt
     1021 tgttccagag ggaagtgatg ttacaacaaa gaatgatggt aaaatttatt ttaaagcact
     1081 tcaagatggt aactataaat tatatgaaat ttcaagtcca gatggctata tagaggttaa
     1141 aacgaaacct gttgtgacat ttacaattca aaatggagaa gttacgaacc tgaaagcaga
     1201 tccaaatgct aataaaaatc aaatcgggta tcttgaagga aatggtaaac atcttattac
     1261 caacactccc aaacgcccac caggtgtttt tcctaaaaca gggggaattg gtacaattgt
     1321 ctatatatta gttggttcta cttttatgat acttaccatt tgttctttcc gtcgtaaaca
     1381 attgtaaggt gtcgttgaaa ttattaaata atagaaaatg actagttttg gcctttccct
     1441 attgtcagtc agattagtta ttatcaagct tctcatgcca atattaatgc ctttaaaaga
     1501 acagttacaa ccattgaccg tacggagatt caacgacggt taggtctggc taaagcctac
     1561 aatgctagta tttctggaac aagtagtcag tcgactcaat ctgtgctgag agattcttat
     1621 tctgaggagc aaaaaaggca gggtggactg aatacgctag gatgttagaa gtcagagagc
     1681 aggttgacca tgtgatgatt ccaaaaatca atcaggattt accaatctac gctggttcag
     1741 aagaggacaa tctgcaacgg ggagttggtc atctagaagg gataagtttg ccgattggag
     1801 gggcttctac acatgcggtc ttgagcggtc aaagaggtat gccagctgct cggttgtttg
     1861 cggatttgga taagatgaaa aaaggtgatt atttttatgt taccaatctg aaagaaacct
     1921 tggcttatca agtggatcgt atcatggtga ttgaacctag ccaattggat gccgtgagca
     1981 ttgaagagga taaagattat gttacccttc tgacctgtac accttatatg ggctctttgt
     2041 caactgtaat gggtgactta tcattaacaa cgagagagaa tcagcttggt tctctctctt
     2101 tttggatgtt caaagcgatg agaattttgc ttttaaaatt tttaaagttg cgaaagccaa
     2161 aggcttgtcg cttgatgtct ttgataagct tgttggtcgc ttctaatttg gcattggaaa
     2221 gggatagctg agggcgtttg tgatgtaagt tttgtacttc atgaaggtcc taaagaccat
     2281 tttaaaagca gaattgccag ttgttacgtt atcatctatg aagctaaaga attcagttgt
     2341 tcgcttttct tagaaatgaa agagtatgag ctgataaagg tcgttataat agcggagctc
     2401 atctgagaaa acttttatac ctcaaagtca gtctagcttt gatatcatga agccattagg
     2461 agttattcct tatcttttag tggcgcgcga tccatatagt gatagatcga gatatttaga
     2521 tccaaaagtt ctatcatcct cttttggcgc cttttttcca gcagataata ttaaggtagc
     2581 ttggtctaac aactccagca ctttatttac accacctatt aatgcaaact acaccactca
     2641 gattcaagct attgggacaa cgattaagtc acaaattccg gaatcgattt tgacggttac
     2701 ggataaaaag agcaggaagt tcagcattaa caagattgac gaagctaaag aaggcttagt
     2761 aggtgcgacc ttcaccttgt ctaaacgcac aacagtagcg gcagatcatc aagtacaagg
     2821 agatttcatt cctgtcagca aagagacgac agtcggtcgg acaaccctta cctttgacaa
     2881 ccttaaacct ggattttatg accttaaaga aacgaaagcg ccgaatgctt acgtacttga
     2941 tcctaagact tatgttgtgg tcgttcaaaa ttcaggaaaa acgacaattg tggatgaagc
     3001 taacttcaaa gaggctgatt acccaatggc tgataatacc agccatgtgg agtgcgtagc
     3061 gttgctacaa cgaagcaaag ggtaaaaatc ctttatttta agcacttttt caagcatttt
     3121 gtctttattg aaaagagtga ttttaacata aaaaaggtat taaaaaacat attgacgtga
     3181 ccgtttgttt tgaagtggct tgcgtagaca aaaaaataga tacgtcagat aaatttctgg
     3241 cattacgaga acatttttag agtgttctct ttttttagtt tacggaggaa aaatatatat
     3301 ggaaaaacag gattcacgag ttctcatcca ttgggagggg aattctgggg acaagctcat
     3361 tgaacaccaa accagcgcaa cggggtggta ctaccaagtc gatcgtagct ttagtcaacc
     3421 aaaaggggaa ccgcccagaa tgatccaaag gcactagaaa gtgtccgtaa tgattcgatt
     3481 tcgggcggtg atgatgtcat gggttatgct tatagcaaat gtacttgggg agttgcggca
     3541 cgaattaatc agtgggactg aaactcaaag gttgaaatgg tgagaagatt accattacca
     3601 gttcaatggg aaatggtcag gattgggttg gaacagccga aagactagat ggtgaaactg
     3661 atacagttcc aaaagaaggt actattctct ctttttagga aagtagttat ggttcgtata
     3721 taggctacgg aactatatct ttcgtcacat tacatctaca gatagtacca tgaattttgc
     3781 ttatatgacc aagtaaagtg aggatatact aacaaatgaa atatttatta tcgtatttgt
     3841 ccattttatc gaaaagtttg catattatca ttatgtttga taagatgcaa atataatgat
     3901 agta
//
LOCUS       DATROY010000149         3862 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.150, whole genome shotgun sequence.
ACCESSION   DATROY010000149 DATROY010000000
VERSION     DATROY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            356..688
                     /locus_tag="VBD11_001815"
     CDS             356..688
                     /locus_tag="VBD11_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954729.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKKE"
     gene            690..1199
                     /locus_tag="VBD11_001816"
     CDS             690..1199
                     /locus_tag="VBD11_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954730.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
     gene            1370..1756
                     /locus_tag="VBD11_001817"
     CDS             1370..1756
                     /locus_tag="VBD11_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO6954731.1"
                     /translation="MKKKYWILLILLLTVLSGGGYMVFKQHTEKEKMIAIAHSAEAMQ
                     IYEKHMKANDSRAFSEQGIIHNYKINDSELNYNPMGGMEVTVYVNNDTNKYFQFTILD
                     NGNGKLESGSYIISADLDNDLETNKK"
     gene            1793..3010
                     /locus_tag="VBD11_001818"
     CDS             1793..3010
                     /locus_tag="VBD11_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6954732.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            2998..3399
                     /locus_tag="VBD11_001819"
     CDS             2998..3399
                     /locus_tag="VBD11_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954733.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            3383..3688
                     /locus_tag="VBD11_001820"
     CDS             3383..3688
                     /locus_tag="VBD11_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954734.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
BASE COUNT         1523 a          471 c          752 g         1116 t
ORIGIN      
        1 aagaacactg atatgacatt aaattatgct agtaaaaata gtcctttatt caaatcatat
       61 gaaaaaggaa cagcaaaatt taaagaggaa aattaaatta tattattaac tgctgaataa
      121 gttatggtgc aagacatact tgtgggagtg ttaaggttca agaaggagta gtattgaatc
      181 ttcaattatt tgggaatctg aattaccttc ttcaagaaaa aagaggtaat ttaattaatt
      241 taaaagaagt tatctatatt tgaatggtat agaaaaaaca gaggtttatt tgcttatgaa
      301 aaaacgtata tggtatttga taataataat cacagtaatt ttaggaggac tagccatgaa
      361 aaacttattt gcaacaacag aagcatcatc aaggaaacag gaacaagata gaattgtcaa
      421 ttacataaaa caacatgttg agttaacaaa tggtaatcaa ataaaaaaaa ttgagtttat
      481 cgactttcaa aaaaatgaga tgacaggtac atggggaatt tctactaaaa ttaatgaaca
      541 attttcgatt agtttttctg aagatagaat tggtggtaaa cttagagcat taggatatca
      601 accgaatgaa ataggttttt caaaggacat caatagtaat aatcaaaatg ttaatgatat
      661 tgaagtgatt tatatgaaga aagaatagaa tgatttcaga agaaagttac agatatttag
      721 ttgaggatgc ttatcgagtt gattcaaaaa aagtaaaaat acctctgaaa agaagtgata
      781 ttgtagggaa cagtgactat gttattattg aaccacctat agataatact tcaaatggta
      841 tgcaagctat ggtagttgct ccgataaaag aaggaatgac agctgaacca gatactagtg
      901 aaatagtgat tgtttatgca ggtactaatt taggtgagcg tttagacata gctacagatg
      961 ttgaaatggt tgctggaggg gatacatatt tacttgctga tcctaaaacc aagactttta
     1021 ggaaatctca gggaaaatca gcactagagt acgcagaaaa aatatcgagt aaatatccta
     1081 attcagaaat tactacaacc ggtcactcct taggggaatc agaggcacta tatgtcgctt
     1141 taaaaatggg ttggatgaat gttggttata atggtccaga tattcataac atgatataaa
     1201 atgacgaaat agattatatg aaatctcatc cagaataatt tagaaaaaaa tcgaaaaata
     1261 tgagttgttt gagttaaaat tttaactatg gagatagagt cctgtattat ttgattttgt
     1321 agtaaactag ttaggaattt attcatagga tattataaag gagttaatga tgaagaaaaa
     1381 atattggata ttactaatat tactattaac agttttatca ggaggcggat atatggtttt
     1441 taaacaacat acagaaaaag aaaaaatgat tgcaattgca cacagtgcag aagcgatgca
     1501 aatttatgag aagcatatga aagcaaatga ttcgcgagct ttttcagagc aaggaattat
     1561 tcataattat aaaattaacg attcagaact caattataat cctatgggag gaatggaggt
     1621 tactgtttat gtgaataatg ataccaataa atattttcaa tttactattt tagataatgg
     1681 aaatggtaaa cttgaatctg ggagctatat tataagtgca gatttagata atgatttaga
     1741 aaccaataaa aaatgaaaaa aatatattga tgaagaaaaa ttacaaatcg caatgtcaga
     1801 atataaaaca atccatccca acgccagatt aaaggatcaa tatgataaca caattggagt
     1861 tgtttcggag gtttacgaca atacttctca ttctggagaa cagatttatg ctgtggttga
     1921 taatcgtcat attccaaaag aagatgtaaa agaggtgacg gtactttttc gtggatcaac
     1981 atctcctcag gaatttatta ataatcctgc agatgtgact ttggattggt tagaaaatga
     2041 cattcctatg gcaagtaata tttgggtaat gaaagatttt gggaatcctc ataatttcag
     2101 tgctgtatca ccacagttga aagcttcatc agaacattta aaagagatta tgaaaaaata
     2161 tccaaatgca aaaataaaca ttggtggaca ttcattagga ggtatggatg ctcaatatgc
     2221 tgttgtagat attacagata agaaagactt gaaacggatt aactctgtac atgtttataa
     2281 tgctccagat atatatccta cacttactaa aggacagaaa aagacggcag attcactgaa
     2341 atctaagatt gttgtttatg ttgatcctaa tgattttatt ggcatggtgg ggcgtgaagg
     2401 aaaaaaagga agtgaagact ctgtaggaac agtctattat acagaaagtc cagatatcaa
     2461 ctggatagat caacacatga cctatggcta tcgtatggaa aatggtcaga ttaaagttat
     2521 tgaaacgaat ttaccaccgg aagttaggac tactagaaaa gctatgggat ctttttataa
     2581 ggacaagaaa aaatttaaaa aatctggtaa gggtctaagt tcgcatgaaa aaatcttctt
     2641 ggatgctgaa caagctgctg ttattgcaag tggtttagtc tctactgctg aaactgcttt
     2701 agaggatatc aagacatctg cgaaaacagc tgttggagaa gcagaagaat tgtggaatac
     2761 gactaaatcg attccttttg gtattacgga gcttacagaa gcagagttgg cagaagctta
     2821 tgcagaaggt ggtgtcactt atgatagcat agtaactaaa actgatactc attttgatga
     2881 gaaagtcaaa aatgcaggag atttagtaac aacatatacg actttgaaaa gtgatattca
     2941 atcaggtgtt gaaaccatgt tatctaagga tagtgaatta gctggagatt ttaaagcatg
     3001 ggaaaattag acgctaaaga tgataaatta agtaacagta aaaaggattt agaaaagttg
     3061 gaagatgatt accatcataa aaccatagct atttcagata aattctttga actggaagat
     3121 aaacgatgtg agtttgagac gatgcttcag gaaacttacg aagcaacaag ttatcatctt
     3181 cgtcaggatg agatgattaa tgaagagtcc tttatgacca tgaatcacat tatagaagct
     3241 tttcaaagtg attttgatac agaatatact aaagagaaaa gaagattgac tgccttagaa
     3301 gaagaaacta atcaaaagta ctctaaaaag agacaattgc ttgaagaaaa aatagatcat
     3361 ttaatgtcag aaaggagaga ctatgggaat ccttggtgat tatgttttat cagaagcctc
     3421 tgattatatc agtggaaaaa aagatatcaa ggctttaaaa aagaaacttg ataaaatgtt
     3481 agtgacaggt gtttatgctc caagaatcaa gtctaaacgt agaagtattg tctctaccta
     3541 tgatgcagag ataacgacta ctgcaacaaa cgcaaaagct agtataactg ccattgcagg
     3601 tcaaatagac actgcaataa agggacagtt tagaactaaa gttgaaactg tattggataa
     3661 taacagtaca aagtatgatg aaatttaaaa gacatgggct aaataattta gctctttttt
     3721 tatactcaaa aataagagaa atacaaaaca aaatacatat ttatgcaagt aaagagaaat
     3781 aaaaaaatca ccattttatc tgtcaaaaaa aattggtggg caagtgtaaa aagagcgtca
     3841 aaaaagccta taggtatagg ct
//
LOCUS       DATROY010000150         3834 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.151, whole genome shotgun sequence.
ACCESSION   DATROY010000150 DATROY010000000
VERSION     DATROY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            107..919
                     /gene="dhuD"
                     /locus_tag="VBD11_001821"
     CDS             107..919
                     /gene="dhuD"
                     /locus_tag="VBD11_001821"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6954735.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            936..1574
                     /gene="dhuI"
                     /locus_tag="VBD11_001822"
     CDS             936..1574
                     /gene="dhuI"
                     /locus_tag="VBD11_001822"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6954736.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            1600..2607
                     /locus_tag="VBD11_001823"
     CDS             1600..2607
                     /locus_tag="VBD11_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6954737.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            2619..3257
                     /locus_tag="VBD11_001824"
     CDS             2619..3257
                     /locus_tag="VBD11_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6954738.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         1220 a          722 c          712 g         1180 t
ORIGIN      
        1 gccaagtttt ataggagaag aactctatag ttgacaaaat cggtttactt tgttactatt
       61 catgtaaaca ggtttacttc ctgaattttt ttacggaggt catattatga ctgaacaatt
      121 cttaaaagac aacttttcac tagaaggtaa agtcgcctta ataacaggcg cttcttacgg
      181 cattggtttt tcaatagcaa ctgcttttgc tagagctgga gcaactattg tcttcaatga
      241 tattaagcag gaactcgttg acaaaggcat ctccgcctat aaaaaacttg gcattaaggc
      301 ccatggctat gtttgcgatg ttacagatga agatggaatc aacgaaatgg tgaacaaaat
      361 ttctcaagat gttggggtaa ttgatattct agttaacaac gcaggtatta taaaacgcac
      421 ccctatgcta gagatgagcg ctgcagattt tcgacaagtt attgatattg atttgaatgc
      481 tccctttatc gtatcaaaag cggtattacc tggaatgatc caaaaaggac atggtaaaat
      541 catcaatatt tgctccatga tgagtgagct tggacgcgaa acagttgctg cctatgctgc
      601 tgccaaaggg ggacttaaaa tgttaaccaa gaacattgct tctgaatacg gttctgctaa
      661 catccaatgt aacggtatcg ggcccggcta tattgccaca ccacaaacag ctccccttcg
      721 tgaacgtcaa gatgatggtt cacgtcatcc attcgaccaa tttatcatcg ctaaaacacc
      781 tgccgctcgc tggggtgagg cagaagattt aggtgcacca gctatcttcc ttgctagcga
      841 tgcatcaaac tttatcaatg gtcacatact ctatgtagat ggcggtattt tagcttacat
      901 cggtaaacaa cctcaataat aaaggagtaa tataaatgaa aattgcatta atcaacgaaa
      961 atagccaagc gagtaaaaat actcttatct acaaagaatt gaaagcggtt tccaaaaaaa
     1021 aaggatttga tgttttcaac tacgggatgt atggcaaaga agaagaaagc cagctaactt
     1081 atgtacaaaa tggtttactg actgctattt tacttaatag cggtgctgca gattttgtta
     1141 ttacaggctg tgggacagga attggagcga tgcttgcttg taatagcttt cctggcgtag
     1201 tctgtggatt cgctgctgat tctgtcgacg cttacttatt ctctcaagta aacggaggaa
     1261 atgctctttc ccttcctttt gctaaaggat ttggttgggg agctgaattg aatctgaggt
     1321 atttatttga acgtcttttc gaagatgaaa aaggaggggg ataccctaaa gagagggctg
     1381 tacctgagca acgcaatgca cgtatcctta gcgaagtaaa acagattact tacagagatt
     1441 tactctctgt tttaaaagaa attgatcaag atttcttaaa ggaaactatt tctggggagc
     1501 atttccaaga atatttcttc gctaattgcc aaaatcaaaa tatagcagac tacttgaaat
     1561 ctgtcttaga ctaaagcgtc tgttttagaa aggactttta tgtctaagat tctttttttc
     1621 ggtgaacctc tcattcgcat cactcccaaa gaaaatgact attttgcaga tagtatctca
     1681 actaaactat tctatggtgg ctctgaagtt aatatcgcaa gagctctaca aggctttggg
     1741 caagatacta aactcctctc agccttaccc aataatccca taggtaatag ttttcttcaa
     1801 tttttaaaag cacagggtat tgatactcat tctattcagt gggttggaga acgtgttggg
     1861 ttatatttct tagaaaattc ctttgcttgc agaaaaggtg aggtggtcta cgaccgcgat
     1921 tatagtagtt tacatgactt taggataaac caaattgatt tcgatcaatt atttgaagga
     1981 gtttctctct ttcattttag tggtattaca ctatcattgg atgagtctat ccaagagata
     2041 acactgcttc ttttaaaaga agctaaaaaa agagagataa caatctcctt agatctcaat
     2101 tttcgcagta aactaatttc tcctaaaaat gccaaaatcc ttttttctca gttcgctacc
     2161 tttgcggata tttgttttgg tattgaacct cttatggttg actcacagga cactaccttc
     2221 ttcaatcgcg atgaagctac aatagaggac atcaaagaac gaatgatttc tcttataaat
     2281 cactttgact ttcaagtcat tttccatact aaacgtctac aggatgaatg gggacgaaac
     2341 cactaccaag cttacatagc aaatcgtaaa caagagtttg tcacttcaaa agaaatcacc
     2401 acagctgtaa accagagaat tggaagcgga gatgcttttg tggcgggagc gctatatcaa
     2461 ctacttcagc actcagacag caagacagtc attgatttcg ctgtcgctag tgcatcttta
     2521 aaatgtgctt tggaagggga caatatgttt gagaccgtta ccgcagttaa caaagtacta
     2581 aatcaatcga aagacattat tcgttagaag ggagaattat gataaaatca aagattttgt
     2641 tgcaattaat gcaaggaaaa atagttgctg tcattcgtgg aaatagccaa gaagaagctt
     2701 ttcaagctgc tcaagcatgc ataaaaggtg gtatttctgc tatcgaaatc gcctatacta
     2761 atagcaaagc tagccaagtc attgaacaac tagtcacaca atatactaac caagaacaag
     2821 ttgtcgttgg ggcaggaact gttctagacg cagaaactgc ccgaatggct attttagcag
     2881 gagcaaagtt tattgtatct cccgcattta atctacagac tgctaaatta tgtaatcgtt
     2941 atgctattcc ttatttacca ggttgcatga ccttatcaga gataacaaca gcacttgaag
     3001 ctggttgtga aataattaaa atattcccag gaggtactct aggaacatct ttcatctcat
     3061 cactaaaagc acctttacct caagtacaaa ttatggtgac tggtggggta aatctcacaa
     3121 atgccaaaga ctggtttcta tctggagtaa cagccattgg tattggtgga gagttcaata
     3181 aactagcagc tttgggggaa tttgataaaa tcacagaaat ggctaaacaa tacaaaatac
     3241 agctaaatct gaattaacac aataaaaaga caaggcaccc ccacacctca atcatgagac
     3301 agaagaaaag aacttttata aagccatttt tctgtagtac cgtttatctt aatcagactc
     3361 attatctcga ttttcttatc agaccgagat aatttttagc taatcttcct gtagacataa
     3421 ccgttctact tccgacaact gatccatgtc ttcaccaaag atatactact tgcccgcaga
     3481 ttgatataag tagtgaagct catcattctc atatcatttc agcaaataat agatttgata
     3541 tcatttttat atccaaaaac ttagcaatga cctatcaggc ttacccgctt ttcctatctc
     3601 aacacaggct aacttagctc ctatcaaata cattttttaa acataattaa aaacatttct
     3661 gattatagtt tattagattt caacagaagt atttttcctg cgtctcagct tttgggtgta
     3721 ccgttatttt gaagttttga tagttgagtg atatcattaa tagtcatttt ttagacattc
     3781 taaacctcct ttctaatttg accgatttta ttttttataa cgactcgttt tact
//
LOCUS       DATROY010000151         3828 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.152, whole genome shotgun sequence.
ACCESSION   DATROY010000151 DATROY010000000
VERSION     DATROY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            227..619
                     /gene="merR"
                     /locus_tag="VBD11_001825"
     CDS             227..619
                     /gene="merR"
                     /locus_tag="VBD11_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HEO6954739.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKKLHSCPIIETLT"
     gene            648..2288
                     /gene="merA"
                     /locus_tag="VBD11_001826"
     CDS             648..2288
                     /gene="merA"
                     /locus_tag="VBD11_001826"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEO6954740.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKIGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIDEANYQAGEIEEVSSLENVALINEDNYDLLIIGSGAAAFSSAIK
                     AIEYGAKVGMIERGTVGGTCVNIGCVPSKTLLRAGEINHLSKDNPFIGLQTSAGEVDL
                     ASLITQKDKLVSELRNQKYMDLIDEYNFDLIKGEAKFVDASTVEVNGTKLSAKRFLIA
                     TGASPSLPQISGLEKMDYLTSTTLLELKKIPKRLTVIGSGYIGMELGQLFHHLGSEIT
                     LMQRSERLLKEYDPEISESVEKALIEQGINLVKGATFERVEQSGEIKRVYVTVNGSRE
                     VIESDQLLVATGRKPNTDSLNLSAAGVETGKNNEILINDFGQTSNEKIYAAGDVTLGP
                     QFVYVAAYEGGIITDNAIGGLNKKIDLSVVPAVTFTNPTVATVGLTEEQAKEKGYDVK
                     TSVLPLGAVPRAIVNRETTGVFKLVADAETLKVLGVHIVSENAGDVIYAASLAVKFGL
                     TIEDLTETLAPYLTMAEGLKLVALTFDKDISKLSCCAG"
     gene            2448..3755
                     /locus_tag="VBD11_001827"
     CDS             2448..3755
                     /locus_tag="VBD11_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO6954741.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCRECGSTISVDEKRSMTKRLLKSIQEQS
                     MSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLVLT
                     NIERRTIYDIKPNRNKETVIQRLLEISDRTYIEYVTMDMWKPYKDAVNTILPQAKVVV
                     DKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNW
                     HVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFN
                     SSAFNKAMLYDTFNWYEVNDHDITDNLGVDFSTLIKNLEKGDL"
BASE COUNT         1338 a          585 c          801 g         1104 t
ORIGIN      
        1 taaatttaaa gggtcttcgg aatatatagt ggaattactt tcttaatagt ttaaaaacaa
       61 ctggtgttta aatcggattt tctggtggga tgttaggata atcaatagta ttatataaaa
      121 gtatcaatca catacgagtg atactttggg aaaaaaataa aatagtccaa tttacttgac
      181 cgtgtactat ggtacatggt ttataatatt cagggggtga aataagatga tttatcgcat
      241 tagtgagttt gcagataaat gtggagttaa taaagaaacg atcagatatt acgagcgaaa
      301 aaatttatta caagaacctc accgaacgga agctggttat cggatatatt catatgatga
      361 cgttaagcgt gttgggttta ttaaacgaat acaggaactt ggtttctctt taagcgagat
      421 ttataaatta cttggtgttg tagataaaga tgaagttcgt tgtcaagata tgttcgaatt
      481 tgtttctaaa aaacaaaagg aagtgcaaaa acaaatagag gatttaaaac gaattgaaac
      541 tatgttagac gacttaaaac aacgatgtcc agatgaaaag aaattacatt cgtgtccaat
      601 aatagaaaca ttaacatgag agattaatta acgaaaggag cttttttatg aataaattta
      661 aggtaaacat ttcaggaatg acttgtacgg gttgtgaaaa acacgtagaa tcagcacttg
      721 aaaagatagg tgctaaaaat attgagtcta gttatcgtcg tggtgaagca gtatttgaac
      781 tgcccgatga tattgaggtt gaaagtgcaa taaaggcgat tgatgaagca aattaccaag
      841 ccggagaaat tgaagaagta tcatcactag aaaacgtggc gttaattaat gaagacaatt
      901 atgaccttct tattattggt tctggtgctg ctgccttttc ttcggcaatt aaagctatag
      961 aatacggtgc aaaagttgga atgattgagc gtggaacggt tgggggaacc tgtgtgaata
     1021 ttggctgtgt tccgtcaaaa actcttctta gggcagggga aatcaatcat ttatcaaaag
     1081 acaatccgtt tataggttta caaacatccg ctggagaagt ggatttagct agtttaatca
     1141 cgcaaaagga taaattggtg agcgaacttc ggaatcaaaa atatatggat ttaattgatg
     1201 aatataattt tgatttaatt aaaggtgaag caaaattcgt tgatgctagt acggttgagg
     1261 tcaatgggac aaagttatct gcaaaacgct ttttaattgc aacaggtgca tcgccttcgt
     1321 tgccccaaat ttcaggactt gaaaaaatgg actatttaac tagtacaaca cttcttgagt
     1381 taaagaaaat accaaaacga ttaactgtaa ttggttcagg atacattgga atggagcttg
     1441 gacaactatt tcatcattta ggttcagaaa taacgcttat gcaaagaagt gagcgacttt
     1501 taaaggagta tgatcctgag atttcagagt cagttgaaaa agcgttaatt gaacagggta
     1561 taaaccttgt caaaggggca acttttgagc gtgttgaaca aagtggagag ataaaaaggg
     1621 tttacgtaac agtaaatggc agtagagaag tcattgaatc agatcagtta cttgttgcca
     1681 ctggaagaaa accaaatacg gattctttaa atttaagtgc agcaggtgtt gaaactggaa
     1741 aaaataatga aatcctgatc aatgattttg gtcaaacaag taatgaaaag atttatgcag
     1801 caggagatgt gactttagga ccacaatttg tatatgtagc agcctatgaa ggtggaatta
     1861 ttactgataa tgctattggt ggattaaaca aaaaaataga tttatcggta gttcctgctg
     1921 ttacgtttac gaatccgacg gttgcaacgg ttggtttaac agaagaacaa gcaaaagaga
     1981 aagggtatga tgtgaagaca tctgtattac ctttaggtgc tgttccaaga gcaattgtaa
     2041 accgtgaaac aaccggtgta tttaaactag tagcagatgc agaaacacta aaagtattag
     2101 gggttcatat tgtatctgaa aatgcaggag atgtcatcta tgcagcatca ttagctgtta
     2161 aatttggctt aacgatagaa gatttaacag aaaccctagc accatattta acaatggctg
     2221 aagggctaaa attagttgca cttacgttcg ataaagatat ttcgaaatta tcttgttgtg
     2281 caggctaagg gaactttttt acaactccct attcaagtga accagctaat gctttaaggg
     2341 attttctaag tgcatttgtg gtcacaaaaa taatttaaca ctgatgattt cgacatcaaa
     2401 tctataattg ataccttaac accgcagagt aataaaggat gaataatatg tcagacttat
     2461 tatccctacc agacattaaa acaatagaac cgccacaaga aaatgaaacc gatatgatgt
     2521 ttaaagttga agcagtcgga ccacctgaac gttgtcctga atgtggtttt gacaagttgt
     2581 acaaacacag ttcaagaaat caactaatta tggatttgcc cattcgttta aagcgagtgg
     2641 gcttacattt gaaccgtaga cgatacaagt gtcgtgaatg cggatctacc atatctgtag
     2701 atgaaaagcg tagtatgacc aaaaggcttt taaagtccat tcaagagcaa tccatgtcta
     2761 agacctttgt agaagtcgca gaaagcgttg gtgttgacga gaaaaccatt aggaacgttt
     2821 ttaaggacta tgtggcactc aaagaacgtg aataccagtt tgaaactcct aagtggcttg
     2881 ggatagacga gatacatatt atccgtagac ctcggcttgt attgactaat attgaacgca
     2941 ggactattta tgacatcaag cctaaccgta acaaggaaac agtcatccaa cgtcttttag
     3001 aaatcagtga caggacttac attgagtacg tcacaatgga tatgtggaag ccctacaaag
     3061 acgcagtgaa cactatcctt ccacaagcta aagttgtcgt agataagttt catgtagtta
     3121 gaatggctaa tcaagcctta gataacgtca gaaagtcttt gaaagcccat atgagccaaa
     3181 aagaaagacg tacccttatg cgtgaaaggt ttatccttct aaagcgtaaa cacgatctaa
     3241 atgaacgtga atcattcctc ttagatactt ggttaggtaa tcttcctgct ttaaaagaag
     3301 cctatgaact caaagaagag ttttactgga tatgggatac tcctgatcca gatgaaggtc
     3361 atcttcgtta tagtcaatgg agacaccgtt gtatgtccag caactctaaa gacgcatata
     3421 aagacctcgt gagagccgta gacaactggc atgttgaaat attcaactac tttgataaaa
     3481 ggctcactaa tgcttatacg gagtcaatta acagcattat taggcaggta gagcgaatgg
     3541 gtagaggtta ctcgtttgat gccttacgag ccaaaatcct tttcaatgag aagctccata
     3601 aaaagcgtaa gccacgattt aattcaagtg ctttcaataa agctatgtta tacgatactt
     3661 tcaattggta tgaagtgaat gatcacgaca ttacagacaa cttaggtgtc gatttttcca
     3721 cacttattaa gaatttggag aagggtgatt tataagccct tttccaccat aaaatccgaa
     3781 tacccaattt aaaaagataa tagttgcttt tatagttata gctaattc
//
LOCUS       DATROY010000152         3722 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.153, whole genome shotgun sequence.
ACCESSION   DATROY010000152 DATROY010000000
VERSION     DATROY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            43..339
                     /locus_tag="VBD11_001828"
     CDS             43..339
                     /locus_tag="VBD11_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6954742.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
     gene            complement(425..964)
                     /locus_tag="VBD11_001829"
     CDS             complement(425..964)
                     /locus_tag="VBD11_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO6954743.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            1103..3421
                     /locus_tag="VBD11_001830"
     CDS             1103..3421
                     /locus_tag="VBD11_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6954744.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            3488..>3722
                     /locus_tag="VBD11_001831"
     CDS             3488..>3722
                     /locus_tag="VBD11_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEO6954745.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEI"
BASE COUNT         1285 a          567 c          694 g         1176 t
ORIGIN      
        1 aacttatctc gtcgaatctt atataactaa ggagatttta ttatgaatct aaatgataga
       61 ttaaaaatag aagaaatgga agagaagtat gatagcttca aacctagaat caatgcatta
      121 gtagaagcta tagatgattt tcaaaagcac tatgaagatt acgtgaaatt aagagaattt
      181 tatggtagcg aagactggtt tagactatca gaacaaacag aaaataatct caaatgcggt
      241 gtactgagtg aggatcagct ctttgatttt ataggagaac ataacgagct tgtaggccaa
      301 tttttagata tgtcttctca aatgtatcgt catttataat gtcagtcctt attagtgttg
      361 ttatcctata atgaggataa aataaaccta actaaaaatg accagcctat aaggaaggct
      421 ggttttaagt ttgaggtaat aaactgagta gcacttgtgt aatttgttga gggctttctt
      481 tttttcctcg tgttatccac atttgataga ctccaaaaat agcatgactg agatagacac
      541 tataatacaa acggttgata tcagactttg ttgcatctgg attgaccaca ggtaattctt
      601 tagaaagcat taattgtaat ttattaagga tgtaggtctg aatttctttt gttccatttt
      661 gggttaagag agcagcaaat aatgattctc tatctaagaa ttgaaaaatt tcgagcaagg
      721 catcttcttt tttaaattga ttgcgatcaa aaatatattc gactttattg aagagtgact
      781 gttggtattg atcaatcatt tcatatttat ctttataatg agtataaaaa ctagaacgac
      841 tgattccagc tgttttagtt agattaatcg ttgaaatttc atcgaagctc tgatctttta
      901 ataacgtaat catagctgct tcaatagctc tttttgtttt ttctctcctc gtatcgttta
      961 ccatattaat taccccttaa tatttttgta catttttaaa cacagtgtcc aaaaacgaga
     1021 tttttatctt gaaattatct atcatcactg tataatatat tttatcaaaa aaatgaacaa
     1081 tatgtccaaa ataggagaaa aaatgttaga cgaattaaaa gcaatcataa aaagcccaaa
     1141 attatggatt acgatggctg gggttgcact gattccaacg ctatataatg ttatctttct
     1201 aagctcaatg tgggatcctt atgggaatac taaaaattta cctgtggcag tcgttaacca
     1261 agataaatcg gctaaattaa atggaaaaac tatttcaatt ggcaaagata tggaggacaa
     1321 tttgtctaaa aatgatagct tagatttcca ttttacaaca gctaaaagag cagaaaaaga
     1381 attagaaaag ggtcactatt atatggttat tacctttcct aaagatcttt ctagaaaagc
     1441 gacaactctc atgacagaaa aaccagaaag gttaaatatt acttataaaa ctactaaggg
     1501 gcgtagtttt gtagcttcaa aaatgagtga gacagcagct aataaactaa aagatgaggt
     1561 agcagaatct attacaggga cttatactga atcagttttt aaaaatatgg gttctatgaa
     1621 aacaggtatc aataaagctg ctgatgggag tcaagaatta ttaaatggtt ctaataagct
     1681 acagaatggg agccaaacct tgacaagtaa tttagatgtt ttagcaagct ctagtcaaac
     1741 tttttctgga ggagctaata aactaaatag tggaataaac ctttatacag atggtgtggg
     1801 aaccctatct aacgggttag aaacattatc agatggtgtt actgcttaca caacaggtgt
     1861 gcataagttg agtgaaggta gtcaaaaatt agatgataag tctcaagcat tggtggaagg
     1921 atcagaaaag ttgacggttg gtttacaaca attgtcacaa gcaactcaac taaaaccaga
     1981 gcaagaacgt actttgcaga acctttcaga tggattaaag aatctaaatc agattattac
     2041 aaatttacaa tcgacagcaa ctactgatag cgatactaat agtaagttat ttaatttttt
     2101 atcaactatt gaatcaagta caacggcttt gatgaatacg gctgctgctg ataaacaaaa
     2161 gcaaatgact gcggtacaat caacgtcagc ttttaaaagt ttaacccctg aacaacaatc
     2221 tcaaattacc tcagcggtta ctggaacacc tacttcagct gagacaattg cagcaaatat
     2281 ttcttcaaat atagaaaata tgaaaactgt gcttagtgaa gcatctagtt cagccccttc
     2341 taataataac tcacaaaatt ttcaaacttt gtcaggaaca gctaacaatt tagtattaaa
     2401 agctatctca gatttagata aaattcaaaa attgccaacg gctactaagc agttatatca
     2461 aggtagtcaa accttgacaa aaggaatcac ggattatacg aatgctgtag ggcaattacg
     2521 caaaggtgca gaaactttag atagtaaatc caatcaattg atttcaggaa cacaaaaagc
     2581 aagtcaggga gctcaaactt tagatagtaa atcggatcaa ttgagagatg gagcggggca
     2641 attagctagt ggttctgata gaattgcaga tggttctaat aagcttgctg ggggaggaca
     2701 tcagttgaca gatgggttga cagagttgtc aggtggtgtt tctcaattat caagttcttt
     2761 gggtaaagct ggggaccagt tgtccatggt ttctgtaaac aaggataatg ccaatgctgt
     2821 atctagccca gtcactatca agcatgaaga ttatgatagc gttgatacga atggtgttgg
     2881 aatggcacca tacatgattt cagttgcgtt gatggtggta gctttatcag ccaatgttat
     2941 ttttgcgaaa gctttatctg gcaaagagcc agctaatcgt tttagttggg ctaaaaataa
     3001 attattaatc aatggattca ttgcaactct agcagcaact atcttatttt ttgcagttca
     3061 attcataggt cttaaaccag attaccctgg aaaaacctac tttattatcc tattgacagc
     3121 atggactttg atggcattag taactgcttt agtgggatgg gataataggt atggttcctt
     3181 cttgtcgtta ttaatattat tattccagct tggttcaagc gcaggaactt acccaataga
     3241 attgagtcct aagttctttc aaacaattca accattttta ccgatgactt actctgtttc
     3301 aggattaagg gagaccatct cgttgacagg agacgttaac catcaatgga gaatgctagt
     3361 aatcttttta gtatcatcga tgatacttgc tcttcttatt tatcgtaaac aagaagatta
     3421 atagaaagta tctagtgata gactaacagt atgatatgct atgtcaaagt atttaggagg
     3481 agaagatatg tctactttaa caataattat tgcaacatta actgctttgg aacattttta
     3541 tattatgtat ttggagacgt tagccaccca gtcaaatatg actgggaaga tttttagtat
     3601 gtctaaagaa gagttgtcat atttacccgt tattaaactt tttaagaatc aaggtgtata
     3661 caacggcttg attggtctat tcctccttta tgggttatat atttcacaga atcaagaaat
     3721 tg
//
LOCUS       DATROY010000153         3717 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.154, whole genome shotgun sequence.
ACCESSION   DATROY010000153 DATROY010000000
VERSION     DATROY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..838)
                     /locus_tag="VBD11_001832"
     CDS             complement(<1..838)
                     /locus_tag="VBD11_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6954746.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIM"
     gene            1096..2310
                     /locus_tag="VBD11_001833"
     CDS             1096..2310
                     /locus_tag="VBD11_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6954747.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
     gene            2318..3700
                     /locus_tag="VBD11_001834"
     CDS             2318..3700
                     /locus_tag="VBD11_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954748.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
BASE COUNT         1103 a          569 c          762 g         1283 t
ORIGIN      
        1 tcataatgta ctctgaaaat aacttattct tatgaaaatg gtagacattg cactctttat
       61 ccaaatagcg atgaatgaga gtttgtggaa caaatgttgc tcccatccct gacaaagata
      121 ggctagctgc tgtaggaacg gtactagttg taatcatctg atttaatgac atgtgatgaa
      181 gctcatagaa ttgtgcaatt gttttgccaa ttccgtactc taatggtgtt aggataagag
      241 gttcattgtc tagaactgaa ataggatatt caaaaggaat aatctgtcca cgtttttcag
      301 cgttgtagag ttggctggtg gtaggtatca ttaaaaacaa ttcgtcgcgg taaatagtgg
      361 ttgatgcaat tccatcttta taaaggatag gagccatccc aacagctagg tcaatatcac
      421 cactcgctac caattgttca gaaatatttt gattgttttc gagtaatttg acagaaattt
      481 ctgggtgctc caagttaaat ttgggaagta catttgccag aatagaactt gctaagccag
      541 agttaatccc aagtgtgatt tctccttttt tatcagaacg gatgagatat agttctttgg
      601 ccatttgacg ctcaatcata tcaatttctt ttaggtagaa tagatatctc tgtccagcat
      661 aagttaaaaa ggtttggtgt ccttggctct ggatgagttt tatttctaac tcattttcta
      721 attgcttgat cactttgctt aggtaaggtt gagaaataaa aaggtgtgca gcggctttag
      781 tgatgtttga gtatagctcg atactctcta gatacattaa tttttgttga tttttcaaga
      841 ctttataacc agtttgatat ccttagatta ataataagtt atgatacgtt atggctataa
      901 gtttactatc attttgagct aaaatcaatc tatttaattt agtagtataa catgatatat
      961 tcataactat ttagttatag aaatatctaa aaaaatttat ttgtaaaaaa ttattttcaa
     1021 aagtagacta tataagagca gattgttaga ataatagcct tattttaact gttcatattt
     1081 taaggaggaa agcaaatggc caatgcatct ttgcgacatc aattatttga aaaacttgac
     1141 caaaaatgtg atcaaatggt tgctattcgt cgctatttac atgaaaatcc cgaattatca
     1201 tttaaagaga caaaaacagc agcttatatt tcagattttt ataaaggtaa ggactgtcat
     1261 gttcagacac aatttggtgg tatgaatggc gtcgtagttg atatctatgg tgataaagcg
     1321 acagataagc ccatcaaaca cattgctttg agagccgact ttgatgctct tccaattcag
     1381 gaagagacag ggctttcttt tgcttcaaaa acagcgggcg tcatgcatgc ttgtggacat
     1441 gatgcccata cagcttattt attgattttg gctgagagct tgattgaatt aaaatcagaa
     1501 ttttcaggtc atatccgtat cttacatcaa ccggctgaag aggtgccacc aggcggagca
     1561 aaagcaatga ttgaagcagg atgtttagat ggcattgatg ctgtccttgg tatccatgtt
     1621 atgtcaacga tggaagaggg aacggtacaa tatcacgcag gtcccattca aacagggcgt
     1681 gccactttta aagttatttt gcaaggtaag ggtggacatg gttccatgcc acatcgtgca
     1741 aatgatacaa ttgttgcagc ttctagtttt gttatggcag cgcaaacgat tgtcagtcgt
     1801 cgagttaatc catttgacac agcagtagtt actatcggat cttttgatgg taaaggttct
     1861 gctaatgtga tcaaagatag tgtaacctta gaaggtgatg tgcgcgtgat gtctgaagag
     1921 acacgaggtg ttgttgagga ggaatttaag cgtattctag atggaatagc acaaacttat
     1981 ggtgttagtt atcagttaga ctatcagaat gattatcctg ttttagttaa taacagtgaa
     2041 gtgacacaga aagttgcaaa tagtttaaaa tctgttgcta taaaagaaat tttagatgtc
     2101 attgattgcg accctcaaac gccgtctgaa gattttgcct attatgctca aacaatccca
     2161 gcttgcttct tctatgttgg agcacatgaa gaggggcaac catattaccc acatcatcat
     2221 ccaaaattcc aaattgcaga atcatcattg atggtatctg ctaaatcaat ggcaacagca
     2281 gcgcttgcta tgcttgtaga aggagaatag ataacatatg ttaacgacaa aggaaaattt
     2341 caaaggaaat aacaaattat tggtgggaat tgtactcgct gtactatcat tttggttatt
     2401 tgcacagtct attttaaaca tgggaccaga tgttcagtca tcattaggta tttcatctgg
     2461 tgcaatggat attggtgtct caagtacggc attgttttca ggtttattta ttgttgtaac
     2521 tggtggttta gcagataaac ttggtcgagt taaatttact tttattggtc tttgcttaaa
     2581 tattattggt tctctactaa ttgttttagc taatggagca gtgctattta ttatgggacg
     2641 tatttttcaa ggattggcag cagcttttat tatgccatca acaatggcct tggttaaaac
     2701 gtactatgat ggtaaagata gacaacgtgc agttagtttc tggtcaatcg gatcgtgggg
     2761 tggctcaggt ctatgttctt attttggtgg agcggtggca agtactctag gttggcgtta
     2821 tgtctttatc ttttcaatca tcgcatcagt agttagtttt ctattaattt taggaacccc
     2881 agaaagtaaa aatgttggac aaaaaactca ctttgattac cttggtttaa ttatttttat
     2941 tatttcaatg ttatcgttaa atattgggat atctatggct caggaacatg gattgatgaa
     3001 cgttattcca ttgagtcttt ttacagtgat gcttattgga tttgttctgt tttattatgt
     3061 tgaaacacgt aaatctaata gttttattga ctttcatctg tttgaaaatc gattttactt
     3121 aggtgcgaca atatcaaatt tcttactgaa tgcagttgca ggaactctga ttgttattaa
     3181 tacttacatg caacaaggtc gccagctaac accaaaggtt gcaggtgaaa tgtctttagg
     3241 ctatttggtt tgtgtactaa ttgctatccg agttggtgag aaaattttac aacgttttgg
     3301 tgcccgtaag ccaatgcttt tgggagctat gagtacattt gttgggatat ttttaatgac
     3361 tttggtcaat attcaaggac ctttgtatct tgtgttagtc tttgttggtt atgctttatt
     3421 tggaacaggc ttggggatct atgcgacacc atcaacagat acagctattt ctagtattcc
     3481 taatgaaaaa gtaggttctg catctggaat ttacaaaatg gcaagttcac taggtggtgc
     3541 aattggtgta gcaacttcaa ttgcgattta tcatgctttt tctggtaatg cagattttca
     3601 caaagcagct ttgtgtggat taattttaaa cctagttttt tgtagtctat cgatcttatc
     3661 aattcttttt gttatcccta agaaaattga ggataaataa ataacaatac ttgagat
//
LOCUS       DATROY010000154         3715 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.155, whole genome shotgun sequence.
ACCESSION   DATROY010000154 DATROY010000000
VERSION     DATROY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..429
                     /locus_tag="VBD11_001835"
     CDS             <1..429
                     /locus_tag="VBD11_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO6954749.1"
                     /translation="NKLSGINETLTSDIDVVFFDKNISYEETVVLEQQLKDNYPQYDW
                     ELKNEFYMNTHSPNTPKYTSSKDAISKFPEKCTAVGERLDDRNQLELYLPYGEEEILN
                     FIVSPTPYFEEDLLRYNVYLKRVDKKKWNNIWPRLTIIKK"
     gene            649..1068
                     /locus_tag="VBD11_001836"
     CDS             649..1068
                     /locus_tag="VBD11_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6954750.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1268..1747
                     /locus_tag="VBD11_001837"
     CDS             1268..1747
                     /locus_tag="VBD11_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6954751.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            1869..2561
                     /locus_tag="VBD11_001838"
     CDS             1869..2561
                     /locus_tag="VBD11_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6954752.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2558..3310
                     /locus_tag="VBD11_001839"
     CDS             2558..3310
                     /locus_tag="VBD11_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6954753.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            3307..>3715
                     /locus_tag="VBD11_001840"
     CDS             3307..>3715
                     /locus_tag="VBD11_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6954754.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIK"
BASE COUNT         1277 a          501 c          746 g         1191 t
ORIGIN      
        1 aataaacttt ctggaataaa tgagacattg acatcagaca ttgatgttgt attttttgat
       61 aaaaatattt cttatgaaga aacggtggta ttagaacaac aattaaagga taactatcca
      121 cagtacgatt gggaacttaa aaatgaattt tacatgaaca ctcattctcc taatacccca
      181 aagtatacta gctctaaaga tgctatttcg aaatttcctg aaaagtgtac tgcagtggga
      241 gaaaggttag atgatagaaa tcaactcgaa ctttacctcc cttatggtga agaggaaata
      301 ttaaatttca ttgttagtcc tacgccatat tttgaagagg atctattacg ttataatgtt
      361 tatttgaagc gagtggataa gaagaaatgg aataatattt ggcctcgttt aacaatcata
      421 aaaaaatgat aacgttactg gagttggctt aacagctttt aaatttttag actgacaaat
      481 agtatagacc tttaataagg tcgtttatcg ataatcatag tgtactgtgt tataatcgtt
      541 atagcataaa aacacgttgc agttggtagt ctgcaagcgt tcagtgaacg tcagtaacct
      601 tccctccagg tcgtccattg caatttaata tttaaaggag ggggctgtat gaaaatgaca
      661 gtctattttg atggtaattt ttggttgggt ttgattgagt acgatgatga tggtgattac
      721 aaagtttttc ggtatttttt tggaaaagaa cctaaagatg atgatgtttt taattttata
      781 aatcataaac ttaatgattt gataaaaaaa tacgagtttg ttaaaacgga tatttctcta
      841 aagcgtacga atgaacacaa aaaaagtcct aaaagaatgc aaagagaaat taatagagaa
      901 aaacgtaagc cagtagtatc tacaaaagcg caattggcga tgaaaactat tcatatgagt
      961 ataaaaaacg aacgtcaatt aagtcagaaa tgtaagaaaa atgagttaag aaagcatcgg
     1021 taccaattaa aacaagaaaa acgttaccaa aagaaaaaag ggcattgatt gtgtcatcat
     1081 ataaaaagct tgagagtatc atctcaagct ttttatatgt taactaatat gagttactaa
     1141 atagtctgat aatgctcaat taatgtgttt tgggaaaaaa tcatttcctt aaaggacacc
     1201 attgtcatgt tgttatcacc ttactttttt tatacttagt atatgtttaa gaaaggaata
     1261 agttgttttg agagattttg aggagttgtt tgataaagta aagccaatcg taatgaagct
     1321 gagaagaaat tattttgttc aactttggga atatgatgat tggattcaag aaggacgtat
     1381 tgtacttttt aggttgcttg aagagcatcc gtatttattg gataatgaaa gcaaactatt
     1441 catttacttt aaaacaaaat tttcaaatta tttaaacgat gtattaagac accaagattg
     1501 tcaaaagcga caatttaata aaatgcctta tgaagaaatc agtgaagtta gtcattatgt
     1561 taagagtaaa ggactagttt tagatgacta tattgcttat cgtgatactt taacaaaagt
     1621 ggaagaaact ttgtcagata ttgataaaga aaaatttgaa aaactgatta atggtgaacg
     1681 ttttgcgggg aagaaaaaat tcatacgaaa tattcaacct tttttcaatg cttttaaagc
     1741 ggattaatcg attttatttc tgcagtagat agctgcagaa tttttttata gggaaagttc
     1801 gctgatatat tcctgtgaaa gcctttgttt tttgttataa tataataaaa tgcttgtgag
     1861 gtgtcagtat gagtaaagtt cgtttatata tcgctcgtca tggtaaaaca atgtttaata
     1921 caattggtcg tgctcagggg tggagtgata ctcctttaac tacttttgga gaactcggga
     1981 ttaaagagct tggttgggga ctaaaggctt caaatattag ttttaaagag gctttttcta
     2041 gtgatagtgg acgtacttta caaactatgg aaattattct cagggaagtg caacaagaaa
     2101 atattcccta tacgagagat aaacgaatcc gcgagtggtg ctttggtagt ttggatggag
     2161 gatatgatgg cgaccttttt aatggcgttc tgcctcgagt ttcaaacgga gatatgagcc
     2221 atttgacaca cgaagaaatt gctaatttga tttgtcaagt tgatacggct ggctgggcag
     2281 aaccgtgggc aatattgagt aaccgtattc tgtcaggctt cactgctata gctaaaaaaa
     2341 tagaggatat tgggggaggt aatgcgattg ttgtcagtca tgggatgact attgcgactt
     2401 ttttatggtt aatcgaccat agtacccctc gtagtttagg cttagataat ggtagtgtct
     2461 cagtcgttga ttttgaagat ggaacatttt ctattcaatc tattggggat atgtcctatc
     2521 gagaaaaagg aagagaaatt ttagaaaaaa cattgcaatg aaaaaaaata aaattatccg
     2581 attcagttta gttggtgttc tacttgcgat actatgcttt agtctttttg ctttattgaa
     2641 gcctaacagt caacaatcat catctcaaaa gttgaggaat gaggatataa aaaagacatc
     2701 ctctcaaaaa agaaataaga aattacgatt accagctgta tcatcaaaag attggaactt
     2761 gattttggtc aatcgtgacc ataaacatga agaattaagt ccagatgtgg tgcctgttga
     2821 aaatatttat ttggataaac gtattacgaa gcaagctact cagtttttag aggctgctag
     2881 agcaattgat tcacgagaac atttaatttc gggttatcgt agtgttgcct atcaggagaa
     2941 gttgttcaat tcttatgtta ctcaagagat gactagtaac cctaatttga cgaggggaca
     3001 agcagaaaag ttggtaaaaa cttactctca gcctgcaggt gctagtgaac accagactgg
     3061 attagcgatg gatatgagta ctgtagattc tttgaatgag agcgatccta gagtagtcag
     3121 tcagttgaaa aagatagctc cacaatatgg ttttgtctta cggtttccgg atggtaaaac
     3181 agcagaaaca ggggtaggtt atgaagattg gcattaccgc tatgttgggg tagagtctgc
     3241 aaaatatatg gtcaaacatc atttaacatt agaagaatac ataactttat taaaggagaa
     3301 taaccaatga ggaaacgttt ttccttgcta aattttattg ttgttacttt tattttcttt
     3361 ttctttattc tttttccgct tttaaaccat aagggaaaag tagatgctaa ttctaggcag
     3421 agtgttacct acaccaaaga agaatttata caaaaaattg tgccagatgc gcaagatcta
     3481 ggaaagtcgt acggtattcg tccttcattt attattgcac aggcggcttt ggattctgat
     3541 ttcggagaga aaatattagc taataagtat cataatctgt ttggtttgct tgcagaacca
     3601 ggaacgccct caattacctt aaatgatagt agtacaggaa aaaaacagga aaagcaattt
     3661 actcattata aatcttggaa gtattcaatg tatgattacc ttgctcatat aaaat
//
LOCUS       DATROY010000155         3654 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.156, whole genome shotgun sequence.
ACCESSION   DATROY010000155 DATROY010000000
VERSION     DATROY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1641
                     /locus_tag="VBD11_001841"
     CDS             <1..1641
                     /locus_tag="VBD11_001841"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6954755.1"
                     /translation="VALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDKIEAVRSQKVG
                     KLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVMGIKGGAAGGG
                     YAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRRVIWKRVVDLN
                     DRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKRLSNIVVAYSR
                     DRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIAHGCNSVLATS
                     TALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRALKMHGGVSKED
                     LSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATLKTLCSNIDVA
                     VELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKKIVTKIYGGNK
                     VHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDITVREFVPKTG
                     AGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            1760..3355
                     /gene="cls"
                     /locus_tag="VBD11_001842"
     CDS             1760..3355
                     /gene="cls"
                     /locus_tag="VBD11_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6954756.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
BASE COUNT         1188 a          546 c          745 g         1175 t
ORIGIN      
        1 gtcgccctaa agcctattgc tgagattgtt gagcaagtag gaattggttt tgatgatata
       61 gaattgtatg gtaaatacaa agcaaaatta tcttttgata agattgaagc tgtaagatct
      121 caaaaagtag gaaagttaat tttagtaact gctattaatc cgacaccagc aggtgaggga
      181 aaatccacta tgagcattgg attagctgat gctttaaata aaattggtaa aaaaacgatg
      241 attgcgcttc gcgaaccttc tttaggtcct gtaatgggta ttaaaggagg agctgctgga
      301 ggtggatacg cacaagtact accaatggaa gatattaatt tgcattttac tggtgatatg
      361 cacgcaatca caactgctaa taatgcttta tcagctctac ttgataatca tattcatcaa
      421 ggcaatgaat tagatattga ccaacgtcga gtgatttgga agcgtgtagt tgatcttaat
      481 gatcgtgctc ttcgtcaggt gatcgttggc ttaggaagcc ctgtcaatgg gataccacgt
      541 gaagatggct ttgatattac tgttgcttct gaaataatgg ctattctatg tttagctact
      601 gacctatcag atttaaagaa acgcctgtca aatattgttg tggcttactc aagggaccgt
      661 aaaccaatct atgttaaaga tttgaagatt gaaggagctc tcacacttat cctaaaagat
      721 gctatcaaac ctaatttggt tcaaaccatt tatgggacac cggctcttgt ccatggggga
      781 ccatttgcta atattgcaca tggatgtaat tctgtcttag cgacttcaac agctctacgc
      841 ttagcagatt acgttgttac tgaagctggt tttggagcag acttgggcgc tgaaaagttt
      901 cttgatataa aaacgccaaa ccttccgact tctcccgatg cgattgttat tgttgctaca
      961 ttgcgtgcat tgaaaatgca tggaggtgtt tcaaaagaag atctctcaca agagaacgtc
     1021 gaagcagtta aaagaggttt tacaaatctc gagcgtcacg ttaacaatat gcgtcaatat
     1081 ggtgttccgg tagttgtagc tatcaatcag tttactgcag atacagaaag tgaaattgca
     1141 actcttaaaa ccttatgtag taatattgat gtggcagttg aattagcaag tgtgtgggaa
     1201 gatggagcag acggtggcct tgaacttgca cagactgttg ctaatgtgat tgaaacacaa
     1261 tcatccaatt acaaacgctt atataatgat gaagacacta ttgaagaaaa aattaaaaaa
     1321 attgttacta aaatatatgg tggtaataaa gttcattttg gacctaaggc acaaatacaa
     1381 ttaaaagagt ttagtgacaa tggctgggac aagatgccta tttgtatggc aaagacacaa
     1441 tatagctttt ctgataatcc aaatttattt ggtgctccaa ctgactttga tataactgtt
     1501 cgtgaatttg ttccaaaaac aggagctggt tttatagttg ctcttactgg agacgtattg
     1561 acaatgcctg gtttacctaa aaaacctgca gctctcaata tggatgtttt agaagacggt
     1621 acagccgttg gattatttta aataataaag aggtagggct tgtgatgcta gcctcttttt
     1681 attggggatt agattttaac tagagataat ttagaataat agaatgctat tcaagtgaca
     1741 gtatgctata ataaaatgat tgatttggag gggggaaaaa cttatcagta ataaagtcaa
     1801 aattgttcgt ttacttaata agagtaagaa aagtttacta aggggtatat ttagtcgaac
     1861 aaccgttatc gctattttat tgattttaca attattgttt ttactagcct cttactcatg
     1921 gctagagcag tatcgtgttt ggttagcaac cgttgagcat atcttaacaa ttggagcggt
     1981 cttatattta gttaatagtg aaatggatgc tttgtcccga gtaacttggt taatattggt
     2041 aatgattgcc cctttactag gggcgatgtt cctaatgtac accaaattcg actggggtta
     2101 tcgtggtttg aaacaaagat tggagacatt gattgatgag agtcagattt atctagagga
     2161 tgatcctgaa actttgaatc agcttaagtc gagtacatcc acaacatatc atttggttca
     2221 atattttgaa aaagcacatg gaaatttccc agtttatcga aatactgatg tgacatttct
     2281 accaacggga gaagcattct ttgaaaaaat gaaagaagaa ctactaaaag ctaagaagta
     2341 tatcttttta gaattcttta tcattgacga aggaataatg tggggagaaa tacttagtat
     2401 tttagaacaa aaggtggaag aaggtgttga agtccgtatc ttatatgatg gtatgataga
     2461 aattacaaaa ttatcctttg attatacgaa acgacttgag aaaataggaa taaaagcaaa
     2521 agcattttct ccgatttctc cttttatttc aacttattat aactatcgtg accatcgaaa
     2581 aattgttgtt attgatggag tagtaggcat gactggtggt gtaaacttag ccgatgagta
     2641 tattaatcac attgagttat ttgggcattg gaaagattca ggtattatgt taaaggggaa
     2701 agcagtggat agtttcctcc tacttttctt acagatgtgg tccattactg aggaaaaaat
     2761 gttagtagcg ccttacctcg gtgtacacga tgatttagtt gaaaatgaag gctatgttat
     2821 tccttatgga gattctcctt tagacactga taaagtaggg gaaaatgttt atatagatat
     2881 tcttaatcat gctagagaat atgtttacat catgacacct tatttaatat tagacagtga
     2941 attggaacat gccattcagt ttgctgcgga acgtggtgta gatgtacgta ttattatgcc
     3001 gggtatccca gataagccaa tcccttatgc tcttgctaaa acttactatc aagcattaat
     3061 aaaatcaggt gttaaaatat atgaatacac ccctggattt gttcattcaa aaatattcct
     3121 tagtgataat acaaaggctg ttgtaggtac gataaatctt gattaccgta gcctgtatca
     3181 ccattttgaa tgtgctgttt atctttataa ggtagatgca atccaagaca tttatcgaga
     3241 ttatatggat acgttaaata aatcaaggtt agttagcttg aaagatataa ataatattcc
     3301 aaaattccaa aaagtaattg gcatagtgac aaaaactatt gctcccctgc tttagaaata
     3361 atttattttt aatttaatat taaaagtaaa ctgaagaatc tagttatatt taaaaagtaa
     3421 aggttgcatt ttaactaaat tatgttaaac tactgttatg cgatgagtcg atatgtggtt
     3481 ttaccactat tgcgcaggga gattataaac gcaggagcgg atcttgataa gttgtgtgaa
     3541 ccttcttatc acacttgaaa aggtgccctt agcttactac tacttgtaat tcattacaaa
     3601 ctgtggtaag tagctgaaaa gcaaaaaaga aagaaccagt ttggttcttt cttt
//
LOCUS       DATROY010000156         3549 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.157, whole genome shotgun sequence.
ACCESSION   DATROY010000156 DATROY010000000
VERSION     DATROY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            391..738
                     /gene="rplS"
                     /locus_tag="VBD11_001843"
     CDS             391..738
                     /gene="rplS"
                     /locus_tag="VBD11_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6954757.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            849..920
                     /locus_tag="VBD11_001844"
     tRNA            849..920
                     /locus_tag="VBD11_001844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:882..884,aa:Arg,seq:cct)
     gene            complement(883..1497)
                     /locus_tag="VBD11_001845"
     CDS             complement(883..1497)
                     /locus_tag="VBD11_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6954758.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(1500..1766)
                     /locus_tag="VBD11_001846"
     CDS             complement(1500..1766)
                     /locus_tag="VBD11_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6954759.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            1766..2170
                     /locus_tag="VBD11_001847"
     CDS             1766..2170
                     /locus_tag="VBD11_001847"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6954760.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            2332..2532
                     /locus_tag="VBD11_001848"
     CDS             2332..2532
                     /locus_tag="VBD11_001848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6954761.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            2554..2814
                     /locus_tag="VBD11_001849"
     CDS             2554..2814
                     /locus_tag="VBD11_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6954762.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            2940..3149
                     /locus_tag="VBD11_001850"
     CDS             2940..3149
                     /locus_tag="VBD11_001850"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6954763.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(3322..3483)
                     /locus_tag="VBD11_001851"
     CDS             complement(3322..3483)
                     /locus_tag="VBD11_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO6954764.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT         1153 a          599 c          674 g         1123 t
ORIGIN      
        1 gtaagaactt aggttaggat aatgtctaca agcatttaag tcgcgatggt gaaatgcatt
       61 agaagataaa aagttgatgt ggatttcaac tttatgggaa agagtcaggt gacacgaaag
      121 ctaatataga aaacgtagtt taaaaaatca tataatatta aatgaattaa actctcaaaa
      181 atcagaaaaa agctaaccta gaaacagtaa atcaaatttc aataatatac aaataactat
      241 tctaaaggat gacaccttgt tcttctagat aaaatagctt tacaagcagg ttaaaatttg
      301 gtagaattgt gagagtgtgt aatggacgca caaaaaacgg ctaatccgct gagacaagta
      361 cttaagatta gtaagagaag gagaataaaa atgaatccat taattcaaag tttgacagaa
      421 ggtcaacttc gttctgatat ccctgagttc cgtgctggtg atactgtacg tgttcacgct
      481 aaagttgttg aaggtactcg cgaacgtatt cagatctttg aaggtgttgt tatctcacgt
      541 aaaggtcaag gaatctcaga aatgtacaca gtacgtaaaa tttctggtgg tatcggtgta
      601 gagcgtacat tcccaattca cactcctcgt gttgataaaa tcgaagttgt tcgttatggt
      661 aaagtacgtc gtgctaaact ttactactta cgcgcattgc aaggtaaagc tgcacgtatt
      721 aaagaaatcc gtcgttaatt ttgatgatca gattttaaaa atgcttggtt gtttgaggat
      781 agtaactatg ttttaaaact ggacaaccaa gacgtaaaaa atctgcctgt gggcagtttt
      841 tttactaggt ccccttagtt caatggatat aacaactccc tcctaaggag taattgctgg
      901 ttcgattccg gcaggggaca tattcattgc atgtaaatag cggtttagag ctattttgcc
      961 ccaaatccgc cccaaatttc tctgattaag tttatcgttc ctatcttttt gttcttgtaa
     1021 ttgatgtgcg taaacttcta aagtgatatt taaattctcg tgatctaaaa cttgagagat
     1081 ggaaattaga tagcttgcaa atgtatgcct gagagagtgc actcgtacct cgcgaccagt
     1141 tatttttcgg atagttttat tgactgcatt atttgaaagt ttgtcgaata atctgtcgtt
     1201 tttatttttt gtaaattcat gcaaaaaaaa taatgtatca ttgtcaattg gtatatttct
     1261 gatactactt ttgttttttg ttggcaggta tctttggttg aaatgataat cccaagtttt
     1321 attaattgat aaatatttgt tagtgtaatc aatatcatta actgttaaac ctaaacattc
     1381 agcgaagcgc atgccagttt tagcgatgag gtataacgct gcatacgatt gatgttgtga
     1441 tttttcttta caaattttta tcaagcgtaa gtattcattg gtttcaagaa attttatctt
     1501 tatttacgcc ccttattttt tgctttaacc ttagtgaata aacaaaaatt tttttctata
     1561 tatccctcgt gaacagccat ggatacgcag gcttttacat gtatgttaaa acgctttact
     1621 gtatcttgca catgcgtttg actataatga tttatgactt gttgatattt agtggaagta
     1681 atattgcaaa gtaatatatt tcctattata tgtttatacg atattcgata ttcccacccg
     1741 ttgtcgcgtt tacggaaata cgccattgat atactccaca ttagctaaag aacagggtgt
     1801 tcaaggctac cttgatggaa aaggctctct tagagatatt tgtaaatggt atgatatctc
     1861 aagtcgctct gttctccaaa agtggataaa acggtatact agtggtgaag acttgaaagc
     1921 cactagtaga ggatatagcc gtatgaaaca aggaaggcaa gccacatttg aagaacgtgt
     1981 agagattgtt aactacacca ttgcccatgg gaaagactat caagcagcta ttgagaagtt
     2041 tggtgtttcc taccaacaaa tttattcttg ggtgcgtaag cttgagaaga atggctcaca
     2101 aggtttggtt gatagacgtg tgaaagggtt ggagagtagg cctgatttaa ccgagattga
     2161 gcaactttaa ctcaagatta aacaattgga ggaacgtaat cgtctcttag aaatcgaggt
     2221 tagtttacta aaaaagttag aagacatcaa acgaggaaac agacggtaag actaggtaag
     2281 catttagcgg agttccaagt aatcaagaat tattacgatg aggaatctaa tgtgcctatt
     2341 caggccttat gccaactctt gaaggggtct cgttcaggct attacaagtg gctcaatcgt
     2401 caaaaaacag attttgagac aaaaaataca aagctaatgg ctaaaatcaa ggaacttcat
     2461 agactctaca atggtatctt aggttatcgc cgtatgacaa catttattaa tcgtcaactt
     2521 gggacaactt aaaacaagaa acggattcgt tgattgatga acattctggg gattagttca
     2581 gtcattcgtc gtgttagcca tgcttgtaca aaagctggtg acagatttta cgaagaaaat
     2641 attcttaatc gtgaatttac agccacagct cataaccaga aatggtgcac agatgtcacc
     2701 tatcttcaat acggtctggg agctaaagct tatctcagtg cgattaaaga cctgtataac
     2761 ggttctatta tcgcttatga gattagtcac aacaatgaaa tccacttgtt atgaagacca
     2821 ttaaaaaggg gctagagctc aatccaggag ccacacctat catccatagc gattgaggta
     2881 gtcaatatac ttccaaagaa taccgttata tcatacaaca agctggtctg accttatcca
     2941 tgtcccggat tggcaaatgt attgataatg caccaactga aagtttcttt gggtttttca
     3001 agactgagtc ttaccacctt aagaaataca actcttatga tgagttggtc aatgatgtgg
     3061 cacgttatat cgaattctac aacacacaac gttatcaatc aaaattaaac aatctgactc
     3121 ctctagaatt caggaatcag gttgcataac ttatctttta ttatttgact gtctacttga
     3181 cagggagccg ttcagattgc ttaacctttc taaatttgct aaaatagcta caagaaaacg
     3241 agccatttaa tgcttatttc ttatactgtc ttgcctcacg ctctcctcga ccaaaaattg
     3301 agcgtgaggc tttttgtttc attaaacgat gatatttcca tattcatcag tttgttttcc
     3361 gagagccatc aaagcttcga taaggtcgat aattccagga ataaaggtaa tactaaaaat
     3421 aatatataaa aaaacctggc ctatttttcc tgcgtaaaat ttatgcgctc caatgccgcc
     3481 caaaagaacg ttaataaaac ataaactact atgttagcat aagactttat ttttacaact
     3541 gaatttcat
//
LOCUS       DATROY010000157         3515 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.158, whole genome shotgun sequence.
ACCESSION   DATROY010000157 DATROY010000000
VERSION     DATROY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            110..931
                     /locus_tag="VBD11_001852"
     CDS             110..931
                     /locus_tag="VBD11_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6954765.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1032..1646
                     /locus_tag="VBD11_001853"
     CDS             1032..1646
                     /locus_tag="VBD11_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6954766.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            1806..3245
                     /locus_tag="VBD11_001854"
     CDS             1806..3245
                     /locus_tag="VBD11_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6954767.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSHKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            3336..>3515
                     /locus_tag="VBD11_001855"
     CDS             3336..>3515
                     /locus_tag="VBD11_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6954768.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYE"
BASE COUNT         1169 a          502 c          701 g         1143 t
ORIGIN      
        1 aatagaaccg ttgacgtatc aggtatcaaa tctttttggt ttatatgtga tataatgaaa
       61 gaacatattg agatatttgg caccctcttg aaaggcaaag gttacttgta tgattaaact
      121 aatagcaaca gatatggatg gaacttttct acgctcagat aaaacttatg ataaagcacg
      181 gttttcttct ttattaacgt tgatggagaa gtatgatatt aaatttgtag ctgctagtgg
      241 taatttatac gatcaattgt tgttaaactt tttggaatac cctaatcgta tcgcctacgt
      301 cgctgaaaat ggtggtcgtg tcattgacca agatggcaca ttactaaaag aaacttattt
      361 atctaatgat acagttgcag ctgttttatc ctatttatat cagaactatc ctgagacact
      421 catcagctta tctggtgaaa aacgttcata ccttgagcga agaactccta ttaataggcg
      481 gactgaatta gaatactata tgcctaattt tatctataaa gaccatttgc tgcccttaga
      541 tgatgaccga tattttcaaa tgacactttg ggtaaatgaa aatcttgttt ctgagatgtt
      601 gttagatatt agtgaacact ttaaaaatca tcatatcaga ttgacctcta gtggttttgg
      661 ttgtatcgat gtcttaccag ctgatgtcaa taaagcggat ggtatcgcta ttctccttga
      721 aaagtgggga ttgaaacagg atcaagtgat ggtttttggt gatgggggca atgatgtgga
      781 aatgttgaga gcagcaaata tttcttacgc tatgtcaaat gcgccagaag aaattaaagc
      841 tattgctaaa tatcaaacag ttagtaacga tcaagatggt gtgttagaaa cgatagagaa
      901 ctttcttcac aagatgagtg ataatgaata gaaccacgta gcttttcacg aagcatacta
      961 ttcaattctc aggtgaaatt ttgttataat acaagaaagc ctagagaaat atttaagaat
     1021 gaaggagaac gatgttagaa gttattgaca aagctttaag agattatcaa atgaaacgag
     1081 aacagttttt tgagattaac aatcagacag tacaagaagg tgccattgtt ttcacaggag
     1141 attctatcgt tgagtttttt cctttaaaaa aacatttagg acgtgactat cctttagtta
     1201 atagaggagt agcaggatca gattcttatt ggcttttaga aaacttgaga acccaagtat
     1261 gggaactatt accatccaaa gtatttattt taattggaac aaatgatatt ggtttagggc
     1321 attcacagtc agaaatcata gctaatatca cggatataat tgcagaaatt cgtgcggaat
     1381 cttatatgac agagattaat atactgagtg ttttacctgt tagtgaagaa gatgactata
     1441 tagagcgtgt caaggtaaga aataatcaag caataaaagc tttaaacaaa accttatcag
     1501 taatttctgg cattaattat attgaattgt atgatttatt agttgacgaa aaaggtcagc
     1561 ttgctagcag ttttacaaaa gatggtttgc acctaacaga tcaagcctat gctaaaattt
     1621 ctgaaacgat aaaactccac ctttaaaagt gtttagatac tatttttaag tatttaaata
     1681 cacagaatta gtgaaaagac cttctaagta gaaagttcat gtcacggata gtaccagtat
     1741 ttggcgaatc aatgtcagta actggtcttt tttaatatta tgataagaaa aaacaagagg
     1801 ggtaaatgat ggtaaaacaa gtatttccaa aaggtttttt atggggcggg gcaactgctg
     1861 ccaatcaatg tgaaggagct tacaatgttg atggacgtgg cctagcaaat gtagatgttg
     1921 ttcctactgg agaagatcga tttgcaatta tttcaggaca aaagaaaatg tttgattttg
     1981 aggaaggata cttttatcca gcaaaagaat caatagattt ttatcaccat tataaagaag
     2041 accttgcatt acttgcagaa atgggtttca aaacctatcg tatgtcaata gcatggacgc
     2101 gtatttttcc aaagggtgat gagctatatc caaatgaagc tggtcttcgg ttttatgaaa
     2161 atatttttaa agagtgtcgt aagtatggta ttgaaccttt ggtaaccatc acacattttg
     2221 actgtcctat ctaccttatt aaacattacg gtgggtggcg gagccataaa atgattggtt
     2281 tttatgagcg ccttgtacga gctttattta ctcgttttaa ggggttagtt aaatattggt
     2341 tgacctttaa cgaaatcaat atgattttac acgctccttt tatgggcgct ggcttatatt
     2401 ttgaagatgg tgaaaatcaa gagcaaatta aatatcaagc agctcaccat gagttagtgg
     2461 cttcggctat tgcagtaaaa attgctcatg aagttgatcc aaatagtcaa atcggatgta
     2521 tgttagctgc aggtcaatac tatccaaata cgtgtcatcc acaagattat tgggcttcga
     2581 tgcaaaaaaa tagagaaaat tattttttta ttgatgtgca agcgcgtggc aaatacccta
     2641 attatgctaa aaaatatttt gagcatttag gtatttcaat tcaaatgaca gcagaagatc
     2701 ttgctttgtt aagagactat acggttgatt ttatttcatt ttcttactat tcaagccgag
     2761 tagcttcagg taatccaact gtcagtgaac aggttcaaga aaatattttc gcatctctta
     2821 agaatcctta cttgaaatct tctgaatggg gatggcaaat tgatccgctt ggattacgca
     2881 ttaccttaaa tgctatctgg gatcgttatc aaaaaccgat gtttattgtt gaaaatggac
     2941 ttggagcagt agatattcct gatgagaatg gttacgtaga agatgattat cgtattgatt
     3001 acctccgtca gcacattgct gcaatgagag atgctatata tgtagatggt gttaatctaa
     3061 ttggctatac tacatgggga tgtattgatt tagtatctgc aggaactggt gaaatggaaa
     3121 aacgctatgg tttcatttat gttgatcgca ataataaagg agagggaaca ctcaagcgtt
     3181 acaagaaaaa atcgttttat tggtacaaga aggttattgc aagtaatggc agtcaaatag
     3241 aataaagagc agactaataa ctgttaactc tctttttctc attcataaaa tagtgtataa
     3301 taattgaaag aaattaagat taataaggag atttcatgaa atttgaaaaa cggcaggtct
     3361 attatgttgt cataacattt gctatttgct atgctataca ggcttattgg ggagctgttt
     3421 ctaatatttt aactacgctt cataaggcaa tatttccttt tttgatggga gctggaattg
     3481 cctatattat taatattgta atgtcagtct atgag
//
LOCUS       DATROY010000158         3422 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.159, whole genome shotgun sequence.
ACCESSION   DATROY010000158 DATROY010000000
VERSION     DATROY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(53..126)
                     /locus_tag="VBD11_001856"
     tRNA            complement(53..126)
                     /locus_tag="VBD11_001856"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Asn,seq:gtt)
     gene            complement(156..227)
                     /locus_tag="VBD11_001857"
     tRNA            complement(156..227)
                     /locus_tag="VBD11_001857"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Glu,seq:ttc)
     gene            419..492
                     /locus_tag="VBD11_001858"
     tRNA            419..492
                     /locus_tag="VBD11_001858"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Arg,seq:tct)
     gene            complement(565..1044)
                     /gene="rlmH"
                     /locus_tag="VBD11_001859"
     CDS             complement(565..1044)
                     /gene="rlmH"
                     /locus_tag="VBD11_001859"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6954769.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            1245..2474
                     /locus_tag="VBD11_001860"
     CDS             1245..2474
                     /locus_tag="VBD11_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6954770.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            2572..3345
                     /locus_tag="VBD11_001861"
     CDS             2572..3345
                     /locus_tag="VBD11_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6954771.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
BASE COUNT         1197 a          601 c          572 g         1052 t
ORIGIN      
        1 attttattaa caaaaaaacg ccgacaaatc aacgtttaga tgtgtcttat cactccggca
       61 gcaggactcg aacctgcgac atcatgatta acagtcatgc gctactacca actgagctat
      121 gccggataaa taaaaaactg caaagcagtt atcaatagtc cgtacgggat tcgaacccgt
      181 gttaccgccg tgaaaaggcg gtgtcttaac cccttgacca acggaccata ggttaggtgt
      241 ttccttagta ctcttaatat tatatcagaa aaaaaaggtt tgtctatact tttgttcaat
      301 aatttttaca aaaaaattga acctacttat gagcaaaaaa atatatttcc aactcataaa
      361 tagtacatat gaaaacttga cagcctgcct aatattttgg tagaataaca gagttgatgg
      421 ttccatagct cagctggata gagcattcgc cttctaagcg aacggtcgca ggttcgaatc
      481 ctgctggaat cagataatga aagtctagat agattatcta ggctttttta tttgttttta
      541 gcggttgttt cataaatgga acgattattt gtggtaagga gtgccttgac gaatcataaa
      601 agcacgataa atttgttcca tcaagactaa tttcataagt tgatgaggta aggttaacag
      661 tccaaaactc attaaataat cagcacgttt tttcactttt tgagataaac ccaaactacc
      721 accaataaca aaagtaatag tagagtagcc ggatatcatt ttttgatcga ttagatggct
      781 aaatgattca gatggcaatt gtttcccttc tatagccaaa ccaatgacat aatccctctc
      841 acctatttta ctcaaaatac gttccccttc tatatctaaa attctttgat tttcagatat
      901 actagcttta tcaggtgtct tttcatcagc tagctcaatt gtctcaattt tagaaaagcg
      961 attcagacgt ttctgatact ctgcaacacc ttctttaagg tatttctctt tcaattttcc
     1021 aactgtaatt atttttaatt tcatagctac attataacat aatccacaat attttcacaa
     1081 tttatccaca tgtttatcct tacatatccc cttattttat aggcaatcta taactataat
     1141 caaggttatc cacaacttgt ggatatttcc tttttttagc ttttaaggta taattaagaa
     1201 gcttgtcata agattagtat aaaaatttac ggaggtagct atttgtgaaa aaaaaattag
     1261 tctcatcact tctaaagtgt tctctaatca ttattgttag ctttgctggt ggagcatttg
     1321 ctagttttgt catgaatcat aatgacaata ttccaaatgg tggtgtcact aaaactagta
     1381 aagtaaatta taataacata acgcctacaa caaaagctgt taaaaaggta caaaatagtg
     1441 ttgtttctgt tatcaattat aaacaacaag agagtcgttc tgacctatca gacttctata
     1501 gtcatttttt tggtaatcag gggggcaaca ctgataaggg cttacaagtt tacggtgaag
     1561 gctctggagt catctataaa aaagatggta aaaatgccta tgttgtcact aataaccacg
     1621 tcattgatgg ggctaaacaa attgaaattc aactagctga tggctcaaaa gcagttggga
     1681 aacttgttgg gtcagatacc tactctgatt tagccgtcgt caaaattcca tcagataaag
     1741 tttcaaatat tgcagaattt gctgattcat caaaactcaa cattggtgaa actgctatag
     1801 cgatcggaag ccctcttgga actgagtatg caaattctgt aactcaaggt attgtatcta
     1861 gtttaaaaag aactgtaaca atgactaatg aagaaggaca aacagtttct acaaatgcta
     1921 tccagacgga tgctgctatc aatcctggta attcaggtgg agcacttatc aatattgaag
     1981 gacaggttat tggaattaat tctagtaaaa tttcttctac atcaaatcaa acctcaggac
     2041 aatcgtcagg aaatagcgtt gaaggtatgg gatttgccat tccttcaaat gatgttgtta
     2101 agattatcaa tcaacttgag agtaacggac aagtagagag acctgctcta ggtatttcta
     2161 tggctggatt aagtaattta ccatccgatg ttattagtaa actgaaaatc ccaagtaatg
     2221 ttactaatgg tattgtagta gcatctatcc aatctggcat gccagctcaa ggcaaactaa
     2281 agaaatacga tgtcattact aaagttgacg ataaagaagt agcatctcca agtgatttac
     2341 aaagtttact ctatggccac caggtagggg attccataac agtaaccttt tatcgtggtg
     2401 aaaataaaca aacagtcact ataaaactta ctaaaactag taaagattta gctaaacaac
     2461 gagcaaataa ctaaatcaat tctagttatt ttaactaaga tatagtaaaa ttaaaggggt
     2521 tggtcagata gaccaatccc tttagtaagt ctatagaatc tgaggtctaa aatggaatat
     2581 ttagaaacga tcaacatcaa tcatattgct ccaaatccgt atcaacctcg cctagaattt
     2641 aatacaaaag aactagaaga attagccgac tctattaaaa ttaatggcct tattcaaccc
     2701 attattgtaa gaccgtcagc agtctttggt tacgaactgg tagccggaga acgacggtta
     2761 agagccgcaa agttagctaa attggaaagt atacctgcaa ttataaaatc ctataataat
     2821 gatgatagta tgcaattagc tattgtcgaa aatttacaac gatctaattt gtcacccatt
     2881 gaagaggcta aagcctactc tcaacttcta caaaagaaaa gtatgacaca tgaagagtta
     2941 gcaaaatata tgggaaaatc tcgtccctat atatcaaata cgatacgact actaaattta
     3001 ccgccactaa ttacttcagc tattgaagaa ggaaaacttt cctctggtca tgcgcgtgca
     3061 ctactcagtc taccagatgc tagtcaacaa aaagattggt atcagagaat cctcactgaa
     3121 gatattagtg taagacgact agagaagtta ttaaaacaag aaaagaaaac aaatcataag
     3181 tcacttcaaa ataaagatgt ctttctaaaa catcaagaaa atgagttagc tcaatttcta
     3241 ggtagcaaag tgaaacttac tattaataaa gatggtgctg gcaatataaa gattgctttc
     3301 aataaccaag aagaattaaa cagaattatc aacactctaa aataagggtg ttgattattt
     3361 ttttatttaa tcaaacttat ccacaaggta ttttgctatt tttctgttga ttctctaagc
     3421 tt
//
LOCUS       DATROY010000159         3330 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.160, whole genome shotgun sequence.
ACCESSION   DATROY010000159 DATROY010000000
VERSION     DATROY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..883)
                     /gene="tilS"
                     /locus_tag="VBD11_001862"
     CDS             complement(<1..883)
                     /gene="tilS"
                     /locus_tag="VBD11_001862"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6954772.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESF"
     gene            complement(885..2171)
                     /locus_tag="VBD11_001863"
     CDS             complement(885..2171)
                     /locus_tag="VBD11_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6954773.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(2171..2305)
                     /locus_tag="VBD11_001864"
     CDS             complement(2171..2305)
                     /locus_tag="VBD11_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6954774.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQIIVEKEKIESREK
                     "
     gene            complement(2308..2679)
                     /locus_tag="VBD11_001865"
     CDS             complement(2308..2679)
                     /locus_tag="VBD11_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6954775.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(2666..2938)
                     /locus_tag="VBD11_001866"
     CDS             complement(2666..2938)
                     /locus_tag="VBD11_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6954776.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
BASE COUNT         1027 a          581 c          473 g         1249 t
ORIGIN      
        1 gaaaactctc gtaatcctta tgaagaaaat agcccgattt aatagtatag tcaatattgg
       61 ctttagttct aatcaatttt aatattactc tgaactgttc tttactcact tgtaaatctg
      121 gaaatttgct aatgtaatct tgcaataaaa attcttggac ggaatgagtt tgccttaaaa
      181 aatcatagca gtttgttact tcgatgctcc ttgttaggtc tgttaaagcc gtgaataaac
      241 actctgtttc ctcagagaga gttatgagag aatctttcaa ttgattattt tctctttcca
      301 aagtaggaaa gtagatgttt cttattcgat ttctcaaata atcagacgaa gcattgctag
      361 catcttcaaa atgaaaaata tttggtaata actccttttt atatggaaga aatggtctaa
      421 tcaattgacc atttgcaaaa gcactcactt gcttaatccc tgataaatag cgtagcctgc
      481 ttcctcttaa aattctcata aatacagttt cagcttgatc atcagcatgg tgagctgtta
      541 ctaaagccgt gtaaccttct tcacgcataa cttttgaaaa aaaattgtag cgatggttac
      601 gtgctctatc ttctgaaaat attccttgaa aataagagat aaagacaggg acatcatgta
      661 tctgtcccca ctgtctgata tattcttctt ctttttctga ttcttttcgc tgtccatgat
      721 ttatgtgtgc aataccaata ctaattgata actctttttg atactggtat aaaaattgca
      781 acaaatttat tgaatcaatg ccaccagaaa cagcaattag aactttctga tgagcggtaa
      841 ataacccttt ggataaggta tctttcaaaa tagtgttata catattactt taaaacctgg
      901 taaacgtcgt cagcaatctt agtgatatca tcgtaagatg atttatctgt aaaaataatc
      961 ataataaaag gttgttctgc ataaacaata gccgcatcat gtttgtagtc ataagcatct
     1021 ccaattttat gtgctacttt aactggtata tttttaggga ttcttgtatt atcaaaatca
     1081 gtctttgata ggtaattaac aatatctcca ttttgataat aaattgcttc catcatttta
     1141 cccgcagctt gtggagaaat ctggcgtttc ttcatatccc agtgcatacc agaaattgtg
     1201 tctacttgtg tttgaaacat tgaatcgtat tgattattga cataataacc tagcatattt
     1261 gttgcaacat tatctgattc cttggcagtt gcttcaagta actccttaac tgtatataat
     1321 ttatgatcag cttttttctc taatttccca ctaccacttg gatcatagct attaggaaat
     1381 tgattaacat tatctttgta ttcaaatttg ctgtccaatg acagttttcc taattttact
     1441 tgctcttggc tagcataaag tgttgctaat tttgcaatac ttgcagagta cattgaccta
     1501 tcaatattaa taccagctac tgtctgtgta tttaattgtt tgatgtatat accatattta
     1561 tctttattat atttttccaa taatacttct tgaaccttag ccatacgatt atcagcaacc
     1621 gatagatctt ctactgatat ccaaaaacca ctgtccgttt tataataaat accatgttct
     1681 gtttctgctt gttctactaa atgcactctg gaataaggtt ttaaattatt tttaacttca
     1741 ctaacaccat ttgtgtaagg tgcttcataa gtcacaaaag aaggttttaa ccaataatcc
     1801 aatgagacag attgctgctt agaaactaaa tcattaaaaa tagcttcacg actagccttt
     1861 atgaactgtc cattggacaa agtaaatact ggaattccat ttgaattaat tgtaagggaa
     1921 ataatcgaca ataaagtatt tggaactaaa gtctctcctg ttatggttaa agcttcatct
     1981 tgaaaactgc ttatttcaat ttttgtagtt ggtctctctg gaatagcatc aaaatacatt
     2041 tccttatcca caactgtctg agttaaggcg tatcgtctat tttgagaaag cgttaatggc
     2101 tcctcggtac ttatagctgg gattgataca aaatataagc tacataataa gaaggttaaa
     2161 actttcttca ttacttttct ctgctctcaa tcttttcttt ttctacaatt atttgcctaa
     2221 gttgatagtt cttatcagct aactcttcta gtttgtcatc cgagtaggct agaaaatgtt
     2281 ctattgtttc aattaaatta ttcataatta ttttggtaaa aggtctggta atgggtaaat
     2341 catttcgccg gtcttagaga aataatactt cgctcgagca tatttttgaa cgtaatctgg
     2401 attttttagt tgttttgcta gtaacttctg gttctcagtt ctattagtta atgtctgata
     2461 gtctttcgtt aattttacaa cttcttgacg acgttcttgt aaagttctgt aactcttaac
     2521 taaattataa gtgggtaaaa taaataaaag catgacaaaa ataagaaccc aacccattaa
     2581 acgatttttt cggcgtaact cctcagcttc gtaacgtttt tttagattct catcgttaat
     2641 atattgatta tttaactgaa caacattagg cttgctcatc tgtttctatc cttgtttcat
     2701 taataatttc atacattttg atagcatctt ctttcttagt gctatctttc atttctaata
     2761 ctttaacggt caacaattta ttaccaaaac gaatttcaac ttggtcatta agctttaggt
     2821 cagttgatga ctttgctaat acgccattca ccttgacacg tcctttgtct gcaacttctt
     2881 tagcaacggg acgacgctta atgatacgcg acacttttag atacttgtct aatctcactg
     2941 atgatcacat ccttctatct aaattaactc tactatttta gcattttttt tgaaaaatat
     3001 ctgaactgat aatatttgtc aatataatgt aacaaaaaga gagctattag ctctcttaat
     3061 tctctcaaat atcaagtaaa aaacttctat tttaataacc tctaaaaata tttatgtctt
     3121 tcaaaagttg agatgtttga cgtgaaacat aatgtttctc gtactattct tcttgaacca
     3181 aacattttct aaaagagtat ccaaatttcc ttgcaacttt tcacttagtt attcttctac
     3241 tggtttacgt gacttaatct cgataaaaca ttcggcaaat tttagaagtt cttcaataat
     3301 ttcataattt ttcttatgtt tgatattaaa
//
LOCUS       DATROY010000160         3329 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.161, whole genome shotgun sequence.
ACCESSION   DATROY010000160 DATROY010000000
VERSION     DATROY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            98..1732
                     /locus_tag="VBD11_001867"
     CDS             98..1732
                     /locus_tag="VBD11_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6954777.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            1751..2023
                     /locus_tag="VBD11_001868"
     CDS             1751..2023
                     /locus_tag="VBD11_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954778.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2033..2383
                     /locus_tag="VBD11_001869"
     CDS             2033..2383
                     /locus_tag="VBD11_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954779.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELRRVYNDRVD
                     ELYQKAKQNEAKKG"
     gene            2831..>3329
                     /locus_tag="VBD11_001870"
     CDS             2831..>3329
                     /locus_tag="VBD11_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954780.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFE"
BASE COUNT         1212 a          528 c          624 g          965 t
ORIGIN      
        1 acataaaaat ggtatttggc acatttatga atagcttgaa tcatcctata taaagtaata
       61 gaatttttac cacaagcaag gtagaggaga agaataattg agtaattgga acttagataa
      121 ttttgataac ttacatagct ctttagcaga aagtgcatat aatagtcgac ctaattcatt
      181 tcctgaatta tttgagactg actcagtaac agaagtaaaa ttttctcaac cctcagaaga
      241 taacaaaggt caaatcacac agggtggcac taacctcccc aatgatggta ttgtctacct
      301 gcaaccagat aagtccttaa aatctattga tgaaaatgta aaggtcctca ttccagatgt
      361 caatggaggc tatcacactg agcattatgt tactcattct tatcaaaaag gtgtgcttac
      421 tgacgataag gcaggtttta atgcttacta cctgtcagat actgaaaaaa tagatagcac
      481 tacaaagcac acttatctag ccattcgtgg tagtgatggg attggtctag atacactaaa
      541 cgattgggtt agtaacaatg caatgtttgc tgtctctaac aagtatattc cacaagcgaa
      601 attggctaat aaggcaatga aagaaaaaat tgccgagcta aaaggaaaag cacctggagc
      661 tgttatggat attactggtc actcactcgg tacaattgta tcatctcaag cagtagttaa
      721 tcttagctat gcagaattgg agaatgttgg tcaagtagtc cttttcgatg gccctgatgt
      781 atcaagaagc ttggaaaaaa tggaaggcat ttctgctaaa aaaatccaag aagctgggaa
      841 gcatgttacc tactatgtca atccctttga tatagttagt atgcttaatc gtgaaaagcc
      901 atggaaagac caattcggga aagtaaatgt cattgttcct agtaaatata cgggtacttt
      961 tgatgagcaa tcttctcacg attttggtgc cttccaaatt gatagtagcg gaaatccttt
     1021 agttgcttct gaaagctatc atccagaatt attaacatct ggtcaagact tagctagact
     1081 cgaaaaaaaa ttcataaaga atcttttagg agagcattat gattcggata agctaggacc
     1141 agtactcaaa ggcgctctga ctggggacct gactcgacta attgcaacat taaaagcttg
     1201 tggtatcagt gtcactgtaa acgaagctaa aaaactgtac gataacttcc aaaaagacta
     1261 caaagccata atttccaaag ctaaaaaaga tggcctcgag tgggatcgca aaaacatcgc
     1321 aagcttccat agtaggatca aatcagcatc aggcagtcaa cggattttac ttagaactca
     1381 aatgctctat atggcaacgc aattagcaga agcagatgtt tcagaaaaag tctctgcaac
     1441 aaaaaaacat attagtgaag ctaaggatac gattgtagaa atatcgacat ccactatttc
     1501 atcagctgaa ataatggcta tgcaccttgg ccaatcagaa gttgatgctc tggtttcaga
     1561 tataaaaatg tcaacagttt ggaatgatag cgtagagact tctgattatg aagcacttga
     1621 tcactataaa acaaaaatga ccacatttac gaccaattta gtaacagttg cacaaaattt
     1681 aacagctcaa gatgagcaac tagctggtga tattgtcaca aacttatcct aaaatagaga
     1741 gattaaatga atgtccaaag aattgaaatt gcccttgaat aaaatgttga aagaacaaga
     1801 aagaatagct attaaggcaa tagttgctac agcaaaaagc actattacgg atactggaac
     1861 aaatttaaaa gcaaccatag agacagatgc ccaagaccta aaaaactatg catccaccta
     1921 taacgccaat atcacggatg gtcttgaagg ccagtcagct caagcagcat ctgatttctt
     1981 gacacaactt ccaaaaccag agttagataa tccagtcaac taggaggcta gtatggtaga
     2041 tacaaaaaaa ctgcaagaac tcgatcaaga gtatgatcaa aatctcagaa acatttatcg
     2101 taacagagag caactagagg atgactttca cttgtttatg gcaagaacag atagcctaaa
     2161 agagtctgtc tatcaagcaa ctttaggtca aggttgggaa ttaccccaag aagctcatgc
     2221 acacctatat aatatggatg ataataagga tacattcata tcagaattca atgaatatat
     2281 ggaaaagctt gaagaaaagg aaatagagct tcgaagagtt tataatgata gagtagatga
     2341 attgtatcag aaggctaagc aaaacgaagc aaaaaaagga taaaaatttt ttcaagtaat
     2401 atatataaat atgaaaaata aagaatcttg cagtaaaatg agtatttatt tttcacgacg
     2461 aacagtcact acaaaatcac taaaatctca aaattacttg gtttttaagt taatgtctaa
     2521 tactcaaacc ttgttaaatc aatgtttgtt atctttaaaa agtgctcagt agcactcaaa
     2581 atccagtgtc ctcacggacg tgtcggttcg accccagccg ccggtatact ttaaaagata
     2641 aggtttctag accttgtctt ttttgttttg ttagataatt tattgtgaaa agttacctaa
     2701 aattacttat atttgttttt tattattgta ttttttataa tgtagttaat aacttttcaa
     2761 ggaggtaata ttatggaaat tttaattaaa acacacttat ttatttactc tggaagaaag
     2821 gaaataaaaa ttgaataaat tttttttaga atcagagaga cttattataa ggccactaga
     2881 agaaagtgat tatgatagtt ggtatactag ttttatgaat cgtggaccat ctttaactcc
     2941 atttgatgat gggatacttg atatgtctat gtgtgacatt aattggttta aaaatttagt
     3001 aaatttacaa aggaaagaat gggaaagtga catagtatat atatttggtg tttttctaaa
     3061 aagcggtgag cacgtaggaa tgttaaatat tgttaccctt gcaagagcag atatgcaatg
     3121 gggagaattg ggatatgttt ttcataatca attttggagt aatggctatg catttgagtc
     3181 aatcttggca ttacttaata gtacttatga aaaattgggt tttcatcata ttgaagcaca
     3241 gattactcct gggaatgaac gatcggaaaa gttggttaga agattgggat taacctatga
     3301 gacaactcga aaagattttt catttgaga
//
LOCUS       DATROY010000161         3323 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.162, whole genome shotgun sequence.
ACCESSION   DATROY010000161 DATROY010000000
VERSION     DATROY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.162"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..193
                     /locus_tag="VBD11_001871"
     CDS             <1..193
                     /locus_tag="VBD11_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6954781.1"
                     /translation="ISLLITFLTLIIDSIFHIDLFNSHANATFSQPVLLYNILVSIVE
                     IAIFYMLTHSIIKYKLNIQ"
     gene            complement(230..841)
                     /locus_tag="VBD11_001872"
     CDS             complement(230..841)
                     /locus_tag="VBD11_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6954782.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            934..1283
                     /gene="ssrA"
                     /locus_tag="VBD11_001873"
     tmRNA           934..1283
                     /gene="ssrA"
                     /locus_tag="VBD11_001873"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1552..2190
                     /locus_tag="VBD11_001874"
     CDS             1552..2190
                     /locus_tag="VBD11_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6954783.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(2308..>3323)
                     /locus_tag="VBD11_001875"
     CDS             complement(2308..>3323)
                     /locus_tag="VBD11_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6954784.1"
                     /translation="EFGYISFEKLINTTEMKSVLVCELYHEYLQTMAKSYSFMFTSQR
                     HDLCQLMMTSKRFKNLTLSYYQAEISLEFEKQFAAMVFTIPNIDYHQVVFRGTDANLI
                     GWKEDFKLTYMREISAHRSAIKYLNTILPYFDKVVLSGHSKGGNLALYAAMFTKPDLK
                     AKIDLIWLIDSPGLQKTLLPTTEYKTTKQKCIRLLPEESIVGMMLYSDIEPLIISSNA
                     RGILQHDVTTWEIQEPAILKTGAGLSLKSICFEKTFQQWMAELKSQERKLFFDLLFDS
                     FLSSGVSSLDDFNLASRAKMMKAFHSFRELDDDKKRLFNKSLKLLVTIFWGAYHDNSR
                     ETK"
BASE COUNT         1149 a          567 c          505 g         1102 t
ORIGIN      
        1 aatatcactc ttaattactt tcttaacatt aataatagat agtatttttc atattgattt
       61 attcaactct catgcaaatg caacattctc tcaacctgta ttgctctaca atatacttgt
      121 tagcattgtt gaaattgcta tattctatat gttgacacac tctattatta agtacaaact
      181 taatatacaa taaaaagatt ggagatatat ctccaacctt tttttatttt taattcaaaa
      241 aatttttcaa aatatcaccg ccataaatcc acaaatagct ataaccgatg atagaaatag
      301 gtttcgttat cataatgatt gtcacaaagc gcttaataga catattagat agaccggtaa
      361 tcatcaccat gatgtccgct ggtgaaatgg gagatgccat acaaaagata aaaaactttt
      421 catatccgga tgtttctagc ttagactcat atttatcaaa tgtcttctga tccataaata
      481 gcaaaacgaa ttttcgtcca taaaatttga ctaaccaaaa aaggatgaca cttccgatga
      541 taatcccaat atagttataa ataaagccta aggtaggacc aaaaattaag aaacctgcta
      601 ctgttgtaac acctccagga attactggaa aaacaatctg tacaatttga gccacaataa
      661 aaatcattgg accccaaaat tcatacttgt gtactaggtc ttttaattca ttgctatcat
      721 tcaaaatacc aagtttgtac aaataaacaa ctaagacaag ggtcgctatt aacgctaaga
      781 cacccaaagc cttaatgacc ttctgccaaa agcggtaacg ttttgataat ttcatattca
      841 tgctttaatc ataacacaac tgttaaattt ttgctatttt aaatagtatt tgttatacta
      901 tattggtaag tttttatgct ttattctact tttggggtcg ttacggattc gacaggcatt
      961 atgaggtata ttttgcgact catcggcaga tgtaaaatgc cagttaaata taactgcaaa
     1021 aaatacaaat tcttacgcat tagctgccta aaaaacagcc tgcgtgatct tcacaagatt
     1081 gtttgcgttt tgctagaagg tcttatttat cagcaaacta cgtttggcta ctgtctagtt
     1141 agttaaaaag agatttatag actcgctatg tgagggcttg agttatgtgt catcacctag
     1201 ttaaatcaat acataaccta tagttgtaga caaatatatt agcagatgtt tggacgtggg
     1261 ttcgactccc accggctcca tatcatttta tcataactta ctatacatac ttaaaacctt
     1321 gttattacaa ggtttttatt ttgtttcttt gctcatacat tgcccctatc ttaggaaaaa
     1381 aatacttgga acagactcta acattgaatt aggccacctc attagctcac tctttgtcaa
     1441 tatccgagaa taagttcttt atcattctaa tttatagaat ttattaaaaa agataaggtt
     1501 tttagaggaa aaaatgttat aatctattta gcctacgatt ggaggctagt tatggctagt
     1561 attttaaaaa aacatttaga gaaacgttta gagcttaaaa atgatcctat gaggacagat
     1621 atgagtactg gtgccagtta ccctaaatat ggattctcgg taggaaagta cacatatgga
     1681 tatcaacaat tcttttacga aggcgttaac ttaaaagaaa ttggagcatt ttgctcaata
     1741 gcccagaacg ttactattac tggtatgaat catccaaccg atcacattac cactaaccct
     1801 tttatttact ataagtcaag gggatttata aatgaggata gggcggactt aatagatgaa
     1861 aagaaaaatg gaaaagttat tattggcaac gatgtctgga taggaactaa tgtcactata
     1921 cttccatctg ttacaatagg taatggagca attattgaag caggttcagt tattaccaaa
     1981 gacattcctg actatgctgt tgtcgctgga acacctgcta aaattatcaa atatcgcttt
     2041 tcagaggaag aaataacatt attaaatgca tctcagtggt ggaattggtc agatgaagct
     2101 attaaggaac atattagcga attttcagat aagagggaat tcttcaatac tctaaaaagt
     2161 atttctgaaa acaaaaatca taaactctag aatgacttca tcaaatagca cactaaaata
     2221 ctactcagca taatatgagt agtattttag tatgctattt tgctagttta tttagcacat
     2281 gtttagacta atgcaattct aggaaagcta cttagtctct ctgctattat catgataagc
     2341 tccccaaaat atggttacta ataattttaa agatttgtta aataaccttt ttttatcatc
     2401 atctagttct ctaaagctat gaaaagcttt catcattttt gctctggaag ccaagttgaa
     2461 gtcatccaaa ctagatacgc cacttgatag gaagctatca aataataaat caaaaaataa
     2521 ttttctttct tgacttttta attcagccat ccactgttga aatgtctttt caaaacaaat
     2581 actttttagc gatagtcctg ctccagtctt taaaatagct ggttcttgaa tttcccaagt
     2641 tgtaacatcg tgttgcagaa ttccacgcgc attgctacta ataattagcg gctctatatc
     2701 actataaagc atcataccaa caatagattc ttctggtagt aagcgaatgc acttttgttt
     2761 agtagtttta tactctgtag taggtaacaa agttttttgt agtcctgggg agtcaataag
     2821 ccaaatcaaa tcaatttttg cttttaaatc tggctttgta aacattgcag cgtacaaggc
     2881 taaattacca cccttagaat gacctgagag aactacttta tcaaaataag gcagtattgt
     2941 atttaagtat ttaatcgctg aacgatgagc cgatatttct ctcatatacg tcaatttaaa
     3001 atcttctttc caaccaataa ggttggcatc agtaccacga aatacaacct gatgataatc
     3061 aatattagga atagtaaaaa ccatagctgc aaattgtttt tcaaattcta aactaatctc
     3121 agcttgataa taagataaag ttaagttttt aaaacgtttc gaggtcatca ttaactgaca
     3181 taaatcatgt cgttgtgacg tgaacataaa gctatatgat ttagccatag tttgaaggta
     3241 ctcatgatat aactcacaga ctaaaactga cttcatttct gtagtgttta taagtttttc
     3301 aaaagaaata tagccaaatt cat
//
LOCUS       DATROY010000162         3312 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.163, whole genome shotgun sequence.
ACCESSION   DATROY010000162 DATROY010000000
VERSION     DATROY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1392)
                     /locus_tag="VBD11_001876"
     CDS             complement(<1..1392)
                     /locus_tag="VBD11_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954785.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKIL"
     gene            complement(1643..1960)
                     /locus_tag="VBD11_001877"
     CDS             complement(1643..1960)
                     /locus_tag="VBD11_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6954786.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(1986..2405)
                     /gene="ruvX"
                     /locus_tag="VBD11_001878"
     CDS             complement(1986..2405)
                     /gene="ruvX"
                     /locus_tag="VBD11_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6954787.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(2405..2671)
                     /locus_tag="VBD11_001879"
     CDS             complement(2405..2671)
                     /locus_tag="VBD11_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6954788.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(2873..3271)
                     /gene="spx"
                     /locus_tag="VBD11_001880"
     CDS             complement(2873..3271)
                     /gene="spx"
                     /locus_tag="VBD11_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6954789.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
BASE COUNT         1119 a          617 c          494 g         1082 t
ORIGIN      
        1 caaaattttc tcattatttt tttgcgctgt attaatatgt ggcaaaacaa attgcgaagc
       61 agattttaac cgagtctggc tataaatgct agcaactttg tcccaaacat aaatcttacc
      121 atctctatca atttttgtag tcaaataatg tgaaatgtca cgcctttctt tactaagagt
      181 aacattttga taagtattat aaacaagtaa ccctatcaat aaggaaacta gtaagagggg
      241 caaataatat gttttcttta caaaaaggtc tacgataaga gatttaccag agaaatactg
      301 acgtctatgt gaacgcctgt caatcacttt ttggtaaaga atagtaattt tattactcgt
      361 tagcaaaaga ccaaatggta aagcaggtaa aaagtggtaa aggttaaaat ctcttgaaaa
      421 aatggccatt atagcgtaaa taataaaaat acaggcaata aagttcaagc cgatttgata
      481 agatgctttt ttacgatttt gaataaaatc ccatagtcca aagaggcaac ctgaccctag
      541 aaggacaaca atttgaattg ctaaactaag aaggaatgag tggtttgtcc tagcgaaata
      601 cgtaaagggg taaaaaatag ctttgtcaat ataagaagag ataatctgta gattaaacat
      661 aaagtaacca acagtataag caattaaaat cataccaaaa acaacgcata aaaactgata
      721 gaaccctcgt agtgactgct tttgcttaat attggttgct gttaagagaa caaaagagca
      781 aagccaaaaa atcagtgttt ggggatctat aaatagtgag aatgctgcta aaataccata
      841 aaatataaat gcttcatcaa cgatattatc gacacaaaat ttaattaaaa accatagcga
      901 aatgagcata aaaggtaatg cccactgtat tgggtacata ccaccgaatc ctaagacaat
      961 attcattata tagaaaatca acgtcatgcc tatcgagaca atttcacttt gtgccacata
     1021 ataaactaat ttataaaaat aaattccaga tacatagtaa gcaataaact gtacaataaa
     1081 taaccagata ctagacccta aaaggtaact tagagcaatc gttacatagt ataaaaatcc
     1141 tcctgtagca aaaacatcac tataagggat ctgtcctttg gtcatcatca aacctgtata
     1201 gagattctga gtctgcaaac catttgcaaa ataagtcaat aaaggatttg caattgctaa
     1261 gagagttgac aaaacactat atagtaaaat tgaaaatcga ctagatttct tcattctgat
     1321 agccgttcgt ttactcattg atgggctatt tttatagctc actccaattg gtttttctag
     1381 gttttctacc actatttttc tcctcatatt tacatactac cagtatatca gaaaacctta
     1441 tttagtcaat ttagccaaca cttgaccgta acgaataata tccctaaagt agtggtatct
     1501 tctagacata tattgtttga gaaattcttg ttcttctaat gttagctgag tatctgacat
     1561 taacatcacc tccttttact acatcatcac tattattatt cgaaaaaaaa aacgactgaa
     1621 caattgctca atcgcttcaa tattattctt catctagaaa gctgttgaaa acttcctcaa
     1681 tcatgtccca ttctgcatct gaatcttctg gtataggctg aaggtctcct tcagttccat
     1741 cagcattttc tgtaaatgag taagcttgaa tctcaatttc accttgttcg tcttcttctg
     1801 cacctgcagg aacgaggaga acatagtttt tgccaaactc ttcacgtcca tcaatagtta
     1861 gtaggatttc aaaaagtgtt tcgttcccat tctcatctac aagagtgatt acttcatgtt
     1921 gatgttcatg gttatgaccg tggttatgat tatgtgccat tgtttctcct ttttatgccg
     1981 aatgcttaaa acatgcggtc taaataattt tgtaagatta gttgagctgc taatttatcg
     2041 ataactttct tacgcttacc acgactaata tcagcttgct caactagcat acgctctgct
     2101 tgaacagtcg ttagacgttc atcttgatat tcaactggaa gattgaataa ttcagtgatt
     2161 ttatcgccat atacctgact agcctctaca cgaggaccac ttgtattatt catattttta
     2221 ggtagaccaa ctacaaattt atcaactttg tactctttta ctaactctgc taagcggtca
     2281 aaaccaaaat ttccagattc ttcatcgatc ttaatgattt caagaccttg tgcagtgaaa
     2341 ccgagtggat cactaattgc aacgccaaca gttttcgagc cgacgtcaag tcccattatt
     2401 ctcattacaa atctattcca tttccttgta aataataacg aacgagctct tctacaatct
     2461 catcacgttc atatttacga atttgattgc gagcatcatt gtaacgaggt acataagcag
     2521 ggtccccact caatacgtaa ccaacaattt gattgatggg attgtaacct ttttcttcta
     2581 atgaacgata aacagctgtt aatgtttcac taatttctac tttgttacta tcatctaatc
     2641 taaaacgaac agtttcatct gtaaatccca tacctacacc ttctttcctt taattctttc
     2701 acttattata acttaaataa agtatttgta caagaaattg ataaacacta tattagaaaa
     2761 tcaaaaaggg agcacaagaa accaagaggt gattctttga tttctttact ccctacctta
     2821 taggtgattt ttacaggtta tcatgtgact ctcacttatg cttatctaaa gctcaaagtg
     2881 ctgcacgaag acgagcttct gcattttcaa cgttacgaat agagcgaggt agaaaagcac
     2941 gaatatcatc ttccttataa cctacttgaa gacgtttgtc atcaattaga attggactct
     3001 ttaaaatacg tggattttct tgaataagat caataacttc atttacactc aactcctcaa
     3061 tattacaatt gagcgcctta gcataacgat ttttagaaga aacgatactt tcaataccac
     3121 tttcagtttt tgttaatatt tctaaaattt catcacgtgt tagtgattct ttacctaggt
     3181 tttgttcttt atatggaagt tgatgagcat ttagccaagt ttttgctttt ttacaacttg
     3241 tgcaacttga aatagtataa atcttaatca tgtgccttct cactttctat cccatgatag
     3301 ttatagttta ac
//
LOCUS       DATROY010000163         3250 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.164, whole genome shotgun sequence.
ACCESSION   DATROY010000163 DATROY010000000
VERSION     DATROY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.164"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..394
                     /locus_tag="VBD11_001881"
     CDS             <1..394
                     /locus_tag="VBD11_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6954790.1"
                     /translation="KAENQHELLFGTCQSDVKLTNTQEHILMLLSQEQLTNSDLAKKL
                     NISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAKPIADEHTHHHDNTLGVY
                     GRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            397..1107
                     /locus_tag="VBD11_001882"
     CDS             397..1107
                     /locus_tag="VBD11_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6954791.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            1097..1909
                     /locus_tag="VBD11_001883"
     CDS             1097..1909
                     /locus_tag="VBD11_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6954792.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2120..3150)
                     /locus_tag="VBD11_001884"
                     /pseudo
     CDS             complement(2120..3150)
                     /locus_tag="VBD11_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT          916 a          506 c          631 g         1197 t
ORIGIN      
        1 taaggcagaa aatcaacatg aattattgtt tggtacttgt caaagtgatg taaaattgac
       61 taatacgcaa gaacacattc tgatgctttt atcacaagag cagttgacaa attctgattt
      121 agctaagaag ttaaatatta gtcaggcagc tgtaactaaa gcagtaaaaa gtttaatttc
      181 tcaggatatg ttgaaagcga ataaagattc taaagatgct cgtattactt attttgagtt
      241 atctgaactt gctaaaccca ttgcagatga acacacacat catcatgaca atacgttagg
      301 ggtttacgga agattagtaa atcatttttc aaaggacgaa aaggtcgttt tagaacgttt
      361 tcttgatctt ttctctagag aattagaagg gtaatcatgc gatatattac agttagtggt
      421 cttacttttc agtatgatag cgatcctgtg ctggaagggg ttaattacca tttagacagt
      481 ggggagtttg ttaccttaac aggtgaaaat ggggctgcta aatcaacttt aataaaggct
      541 actttaggta ttttaacgcc caaagtgggt acagttaata tctctaaaga aaacaaagaa
      601 ggaaaaaaat tacgtattgc ctatttacct caacagattg caagttttaa cgctggcttt
      661 ccatcgtcag tttatgaatt tgtgaaatct ggacgatacc ctagaaatgg ttggtttaga
      721 agactgacta aacatgatga agaacatatt agggttagtt tagaagctgt tggtatgtgg
      781 gataatcgcc ataaaaagat aggaagttta tctggcggtc aaaagcagag ggctgtcatt
      841 gctaggatgt ttgcttctga tccagatatt tttgtattgg atgagccaac aactggtatg
      901 gacgcaggga ctactgagaa attttatgag ttaatgcatc ataatgctca taagcatggt
      961 aagtctgttt tgatgattac tcacgatcct gatgaagtaa aggggtatgc tgaccgaaat
     1021 attcatcttg ttaggaacca atctttacca tggcgttgtt tcaatgtcca tacaaacgaa
     1081 atggaggttg aatcagatgc tacttgatat gctttcctat gattttatgc aacgagcgct
     1141 cttagcagtt gtggctatta gtatttttgc tccaatttta ggcatcttcc taattttacg
     1201 tcgtcagagt ttgatgagtg atactttgag ccacgtttcc ttagccggtg ttgcattagg
     1261 cgttgtttta ggtatctcgc ctacgtggtc aactattttc gttgttactt tggcggcagt
     1321 tgtattagag tatttgcgta ctgtatacaa acattatatg gaaatatcaa ctgctatcct
     1381 gatgtcgatg gggttggcta tatctttaat cgttatgagt aaagctcata atgttggtaa
     1441 tgtcagtctt gaacagtatt tatttggttc tattatcact attgggaagg aacaagtgat
     1501 tgctttgttt gttattgcat taatcacttt tattttaacc atcctattta ttagaccaat
     1561 gtatatatta atgtttgatg aggatacagc ttttgtagat ggattgcctg tgcgtacaat
     1621 gtctattttg ttcaatgttg ttacaggtat tgctattgcg ttaacaattc ctgccgcagg
     1681 tgctctattg gtgtcaacta ttatggtttt accagctagt attgctatgc ggttaggtcg
     1741 aaattttaaa actgttattt ttcttgggat gttaattgga tttgtgggaa tggtagctgg
     1801 tattttcctt tcttattatt gggagacacc tgctagtgca acaataacga tgatttttat
     1861 tggcattttc ctattagtta gcttagtagg cttgcttcgc aaacgttaaa taaaaaaatc
     1921 tatcttattc taggatagat tttttattta tctccctgtt tttttcgaat atattttatg
     1981 agggatcagt tgagaaatta tctcgatgag gtatctacta agttttctgg cttaagttga
     2041 tagaaggggc aatgtgaatc tatttttgcg ttttaggata aaaaatacta ccggcaattt
     2101 tgccggcagt aaattgtaat tattgtgctt cagtagtgct gtgatatttg tgttgattta
     2161 acgcttcttg ttcagtcatt tgaacgatat tagcggtatt tgcatttttt atgttttcta
     2221 aactatacca ataagtattt gaacgaccct tatttgcaac gtagacgatt ctatttttgg
     2281 gagctacatt atttgtatct gattcagaag tattagtgtt agcattttct tctatatcag
     2341 atgcaccttc aatagctgct tcgttttcat cagcatcgct atttggatct tcaccttctt
     2401 ctgcctgttt cccatcttct ttgattagca taccagtttt gtaatcaatt tttgcgagtg
     2461 gagacgtatt ttctaatgta acagttgtga cgccgtattc atcaacttgt tctttgccgc
     2521 cgcctaattt gatttctaga agttttcctt gtttatcaat tccaacatac tgaagttcaa
     2581 ctcggctagg tactaaatta tttccttcgt atattggagt cactttgtaa tcaagctaga
     2641 aatttttatg atttgccaac caactatcta atctattttc gtagtataac ataccatcag
     2701 gattattttc gctaaatcct gtatttagat atttggtcat agtaactagg ttctcaggaa
     2761 cattgttcat tcctgaaaat tgataaccaa cgaggtgacc tcggtctata agccaagttg
     2821 ttttaccctt ttcagtagta aatttgtagt tgtgccaacc ggttggatta tacgtcagtc
     2881 tttctctttt tatcttaggc tcatctgcat cccttaattg tatgtgagaa tatgttgctc
     2941 tttctaagtt atctaatgaa cctaccttga tttgcttatt atgagtaaac ggtaagattt
     3001 ttttgaagaa gtacttacat actgtgtctc aacatgggat gtcgtcttat aatgtttagc
     3061 cttagcttca acattctgtt gtaatgcccc aacgcatagt acgacagtta atagtgggag
     3121 tgatttgtat aattgtttag ataatttcat aaacctaaac ccttctaaat gatatttgtg
     3181 aaggcctttc acaagtataa ttttatcatt ttattgggaa aaagtaataa aaaaacataa
     3241 ctagttaatt
//
LOCUS       DATROY010000164         3232 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.165, whole genome shotgun sequence.
ACCESSION   DATROY010000164 DATROY010000000
VERSION     DATROY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1237
                     /gene="gtfB"
                     /locus_tag="VBD11_001885"
     CDS             <1..1237
                     /gene="gtfB"
                     /locus_tag="VBD11_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO6954793.1"
                     /translation="NDDGFLPQNINSPYSFFCNMEGKNGNPLYFNQVPLPDLWEIKGN
                     NIEAEIWDFSIKRAKIFYQEPKYKRQVKNIDWFDNNKKVRYTDHYNRFGWCFARTHFD
                     KNQNVTTKSYFDKDGKEVIVENFRTGVIILNWLNKDYFFDNRVAFLNFYFSLMGWNLS
                     RIWYNSLSTPFFVSYRMTYPGEDILFWQEDIEDTIPANMRVLLESTNTRTQKVIVQKK
                     NTYHKIKSMLPKEQQEKIGYLGFIYPNKKNNKGRKDIFILTNSDQIEHLEVLVHHLSD
                     YHFHIAAYTEMSFKLMSFSQEQNVTLYPNISRTDLDNLFEICDIYFDINHGNEVDDVI
                     RRAFEYNHLIFAFDNTCHNRELVLDSNIISHTTCEQLINLMKNLSGSIMYLLEQQREQ
                     TSNETKERYKEILGGYGNA"
     gene            1230..1397
                     /locus_tag="VBD11_001886"
     CDS             1230..1397
                     /locus_tag="VBD11_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954794.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            1451..2446
                     /locus_tag="VBD11_001887"
     CDS             1451..2446
                     /locus_tag="VBD11_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6954795.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            2454..>3232
                     /locus_tag="VBD11_001888"
     CDS             2454..>3232
                     /locus_tag="VBD11_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6954796.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLID"
BASE COUNT         1160 a          425 c          590 g         1057 t
ORIGIN      
        1 taatgacgat ggttttcttc cacaaaatat aaattctcct tattcatttt tttgtaatat
       61 ggaagggaaa aatgggaatc ctctttattt taaccaagtc ccactccctg acctttggga
      121 gataaaggga aataatatag aggctgaaat ttgggatttt agtattaaaa gagctaaaat
      181 tttttatcaa gagccgaagt ataaacgtca agtaaaaaat attgattggt ttgacaataa
      241 taaaaaagtg agatacacag atcattataa tcgcttcgga tggtgttttg cacgtacaca
      301 ttttgataag aatcaaaatg tgactacaaa atcttatttc gataaggatg gtaaagaagt
      361 tattgttgaa aattttagaa caggagttat aatccttaat tggctaaata aagattattt
      421 ttttgataat cgagttgcat tcttaaattt ttattttagc cttatgggtt ggaacttgtc
      481 tcgaatatgg tacaactcct tatcaactcc atttttcgtt tcttaccgga tgacttatcc
      541 aggagaagat attttatttt ggcaagaaga tatagaggat actattcctg ctaatatgcg
      601 cgtgttatta gaaagtacga atactagaac tcagaaagta attgttcaaa agaaaaatac
      661 atatcataaa ataaagtcaa tgttacctaa agaacaacaa gaaaaaattg gttatttagg
      721 ctttatatat cctaataaaa agaacaataa gggtaggaaa gatattttta ttttgacaaa
      781 ctctgaccaa attgaacatt tagaagtgtt ggttcatcac ttgtctgatt atcattttca
      841 tattgcagcg tacacagaaa tgtcttttaa gttgatgtca tttagccaag aacaaaatgt
      901 aacgctatat ccgaatattt caagaactga tttagacaac ttatttgaaa tctgtgatat
      961 ttattttgac attaatcatg gaaatgaagt tgatgatgtt attagaaggg catttgaata
     1021 taatcacctt atctttgctt ttgataatac ctgtcataac agagagttag tattagatag
     1081 caatatcatt tctcacacaa cctgtgaaca attgataaat ttaatgaaaa atttatcagg
     1141 ctccattatg tatttgctag agcaacaaag agaacaaaca agtaatgaaa caaaagagcg
     1201 ttataaagaa atattaggag ggtatggaaa tgcctaaaag aaatgaatta ctcaataaag
     1261 aaattaaaat gagtattgat aaacttagat ataaagaacc agagagtgaa catgacaagc
     1321 gacctacttt ttatttggta gtacttatac ttgttactgt agcagttata ttgtcgttat
     1381 ttaaatattt tttatagatt cctattaatg gtagaaaaga agcctgttgt tttttagata
     1441 ggggaagtgt gtgaggacat atattacaaa cttgaatgga cattcaatca ctagtacagc
     1501 acaaatagct caaaacatgg taacagatat agcagtaagc ttaggttttc gtgagctggg
     1561 aatacattct tatccgattg atactgattc tcctgaggaa atgagtaagc gtttagatgg
     1621 aatctgttcc ggacttagaa aaaatgatat tgtcatattt cagacaccta catggaacac
     1681 tacaactttt gatgaaaaat tatttcacaa attaaaaata tttggtgtaa agattgttat
     1741 ttttatacat gatgttgtac cgctaatgtt tgatggaaat ttttatttga tggatagaac
     1801 tatagcttat tataatgaag cagatgtttt aatagcccct agtcaagcaa tggtcgataa
     1861 gcttcaaagt tatggcttga cagtaaaaaa aatcttagtt caaggaatgt gggatcatcc
     1921 caccaatatt actttacaag cagttaatca taagaagctt gttcattttc ctgggaaccc
     1981 agagcggttt aacttcataa agaattggag aattcctacc gagttacatg tttatactga
     2041 ccataacatg caattaccca ccactgtggt taaagagcca tatcaatcgg acgaacaatt
     2101 aattatgaaa atgagcgaag ggggatatgg cttagtctgg atggatgacc gtgataaaca
     2161 atatcaatct ctttattgcc cttacaaact gggcgcttat atagcagcag gaattccagt
     2221 gattattcaa aaaggtattg ctaatcaaga tataatagaa aaaaataatt tgggatttat
     2281 tattgaaaaa attgatgata ttagtaatat agttgaaagt actactgaag aggaatatat
     2341 ggaaattgtt agcgatgtca ggcggtttaa tcctttagtt agacaaggat attttacgcg
     2401 aaaactatta accgatgcag tatttagtgc attaaatagc atgtgagggg aatatgaaag
     2461 aactagaaga aatcgtagtt ttccctataa acaaaagttt ggattacatt ttggaaaatc
     2521 attcgtcagt tattcgcttt ggggacggtg aatttgacat tataatggga aaatcgattc
     2581 cttatcaaga ttacaaccca gagttagcag ataggttatt gcaacttctt cataagcaaa
     2641 gtacgaatga tctattagta tgtcttccag atatgtttca aaatcgacaa agatataatc
     2701 aatcggcaca acttttttgg aaaaatcatt ttcaaaaatt tggcaatttc tataggcaac
     2761 attgttatca agattggtat ggttcttctt ttatttcaag gccatatatg gacttacaag
     2821 ataaggagtc tgctgtagaa tcatttaaaa aattaaagtt actttgggac aaacgcgata
     2881 ttttggtagt tgaaggggaa acttcgcggt cgggtgtagg taatgatctt tttaataatt
     2941 gcaatagttt aaaaaggatt atttgtcctt ctaaaaatgc ttatataaaa tatgatgaaa
     3001 ttcttgaggc tgttgaaaga tatgctcaag ggaggataat cttattgatg ctaggtccga
     3061 ctgcaaaaat tttagtaaga gatttatctg atagaggatt tcaagccatt gatatcggtc
     3121 atattgtttc tgaatatgaa tggtataaga tgggagctac tcaaaaaata aaattttctc
     3181 ataaacacac cgctgagttt aacgatgatg aaaatattaa tttaattgac ag
//
LOCUS       DATROY010000165         3209 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.166, whole genome shotgun sequence.
ACCESSION   DATROY010000165 DATROY010000000
VERSION     DATROY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            87..1448
                     /gene="dnaA"
                     /locus_tag="VBD11_001889"
     CDS             87..1448
                     /gene="dnaA"
                     /locus_tag="VBD11_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6954797.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
     gene            1603..2739
                     /gene="dnaN"
                     /locus_tag="VBD11_001890"
     CDS             1603..2739
                     /gene="dnaN"
                     /locus_tag="VBD11_001890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6954798.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            2797..>3209
                     /locus_tag="VBD11_001891"
     CDS             2797..>3209
                     /locus_tag="VBD11_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEO6954799.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLG"
BASE COUNT         1128 a          540 c          538 g         1003 t
ORIGIN      
        1 ttttattctt caacatctgt ggaaaacttt atttttttat ggtacaatat aacaataatt
       61 atccacaaga caataaggaa gaagctatga cggaaaacga acaactattt tggaatagag
      121 tactagagct atctcgttct caaatagcac cagcagctta tgaatttttt gttctagagg
      181 ctagactcct caaaattgaa catcaaactg cagttattac tttagataac attgaaatga
      241 aaaagctatt ctgggaacaa aatttggggc ctgttatcct aacagctggt tttgaaattt
      301 tcaatgctga aattacagct aactatgtct caaacgattt acatttacaa gaaactagtt
      361 tttctaacta ccagcaatct agcaacgaag taaatacttt accaattaga aaaatcgact
      421 ctaaccttaa agagaaatat acttttgcta attttgttca aggagatgaa aatagatggg
      481 ctgtttcagc atcaattgct gtagctgata gtcctggcac gacttataat cctctattta
      541 tctggggggg acctggtcta ggaaagacgc atctactaaa tgctattgga aatcaagttt
      601 taagagataa tccaaacgcg agggttttat acatcactgc tgagaatttt attaatgaat
      661 ttgtcagtca tattcgttta gattcgatgg aagaattaaa agaaaagttt cgcaacttgg
      721 acttactcct gattgatgat attcagtcgc ttgctaagaa aaccttaggg gggacccaag
      781 aggagttctt caatactttc aatgctttac atacaaacga taaacaaatc gtattgacca
      841 gtgaccgaaa tccaaatcaa ttaaatgatc tagaagaacg tctagtcacg cgctttagtt
      901 ggggactccc agtaaatatc acaccacctg attttgaaac acgagttgct attttaacca
      961 ataaaattca agaatatcct tatgattttc ctcaagatac cattgaatac ttagcaggag
     1021 aatttgattc caacgtacgt gaattagaag gagccttgaa aaatattagt ctagttgctg
     1081 actttaagca tgctaaaact attacagtag atatagctgc agaagctatc agagcacgta
     1141 aaaatgacgg tcctattgtt actgttattc ctatagaaga aattcaaata caagttggta
     1201 aattctatgg cgtaactgta aaagagataa aagcaactaa aagaacacaa gatattgtcc
     1261 ttgcaagaca ggtagccatg tacttagctc gtgagatgac agataacagt ctccccaaaa
     1321 taggtaaaga atttggggga cgagatcact caactgttct ccacgcttat aataaaataa
     1381 aaaatatggt tgctcaagat gacaacttac gaattgagat agaaactatc aaaaataaaa
     1441 tcaggtaggc tttgtggata agtatcagaa aaacacgaga cttttccaca ggttgtgaac
     1501 aagttatttt ccttatgaat actaaagtta taatacttat tcacaacttt aacaggacct
     1561 actattacta ctaaatttat actaatatat aaaggatata ctatgattca tttttcaata
     1621 aataaaaatt tcttcttgca tgctctaaca gtaaccaaac gagctattag tcataaaaat
     1681 gcgattccaa tcctttcaac tgttaaaata gaagtgacta gagatgctat cattttaacg
     1741 gggtcaaatg gacaaatttc aattgaaaat actattcctg cttcaaatga aaatgcaggt
     1801 ttactagtaa cgaatccagg ctctattttg ttagaagctg gtttctttat taatattatt
     1861 tcaagtttac cagatgtaac tttagaattt acagagattg aacaacatca aattgttctt
     1921 actagtggaa aatcagagat tactttgaaa ggtaaggatg tcgatcaata ccctcgtctg
     1981 caggaaatga caacagatac tccattaaca ttagaaacta aactgttaaa atcaattatt
     2041 aatgaaactg cttttgctgc tagccaacaa gaaagccgtc caatcttaac aggtgttcat
     2101 ttggttatca gtcaaaataa atactttaag gctgttgcga cagattcaca ccgtatgagt
     2161 caacgcactt tccaattaga gaaatcggct aataattttg atttggttgt tccaagtaaa
     2221 tcccttcgag aattttcggc tgtttttaca gatgatattg aaactgtaga ggttttcttc
     2281 tcagatagtc aaatgttatt tagaagtgaa aatatcagct tctatacacg tttgcttgaa
     2341 ggaaactacc ctgatactga tcgcctccta actaatcagt ttgaaaccga aattatcttt
     2401 aatacaaatg ctttacgcca tgctatggaa cgtgcttatt taatttcgaa tgcaactcag
     2461 aacggtactg ttcgtttaga aattcaaaat gaaacagtct cagctcatgt aaactctcca
     2521 gaagttggta aagttaatga ggaattggat actgttagcc ttaaaggtga tagtttaaat
     2581 attagtttta atccaactta cctaattgaa tctttaaaag cagtaaaaag cgaaacagtt
     2641 acgattcgat ttatttctcc agtacgtcca tttactttga cccctggtga agatactgaa
     2701 gatttcatcc aattaataac tcctgttcgt actaactaaa taagaaaact acccaagtta
     2761 ttcttgggta gttttcttat tctaagatat gctataatga tgagtaatat gactctatat
     2821 ataatagcta acccccatgc tggtaataaa aatgcctcca ctattgttgg taaaattcag
     2881 gagctttatc atactgaaga tatttctgtg ttctatacag aacagaaaga tgatgaaaaa
     2941 aaacaagtca ttaatatact aaggtctttt aaagaaagtg atcatctaat gattatagga
     3001 ggagatggta ccttatcaaa agtaatgact tatctccccc aacatattcc gtgcgcttat
     3061 tatcctgttg gttcaggaaa tgattttgcc agggctttga aaattcctaa cctcaaagaa
     3121 acccttaccg ctattcagac agaaagatta aaggaaatta attgctttat ttatgataaa
     3181 gggctgattt tgaatagttt agatttagg
//
LOCUS       DATROY010000166         3138 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.167, whole genome shotgun sequence.
ACCESSION   DATROY010000166 DATROY010000000
VERSION     DATROY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..135)
                     /locus_tag="VBD11_001892"
     CDS             complement(<1..135)
                     /locus_tag="VBD11_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6954800.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     F"
     gene            294..1061
                     /locus_tag="VBD11_001893"
     CDS             294..1061
                     /locus_tag="VBD11_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6954801.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(1120..2460)
                     /locus_tag="VBD11_001894"
     CDS             complement(1120..2460)
                     /locus_tag="VBD11_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6954802.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(2560..2976)
                     /locus_tag="VBD11_001895"
     CDS             complement(2560..2976)
                     /locus_tag="VBD11_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6954803.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(2980..>3138)
                     /locus_tag="VBD11_001896"
     CDS             complement(2980..>3138)
                     /locus_tag="VBD11_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6954804.1"
                     /translation="KLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLM
                     QRWLDNMK"
BASE COUNT         1106 a          595 c          478 g          959 t
ORIGIN      
        1 aaaataactt ttcacacgat tcttaagatt tttagcctta cctacataaa taattgtccc
       61 attcttatct ttatgaaggt aacaaccagg actgtctggt aacaattcta gcttatgttt
      121 aatcagttca ttcatagaac tattataaca aaatgtactg taatctttac gaaataaata
      181 ctattcccca acatctattg tatcttccaa tacttgaata aaaccccaat actcctagtc
      241 taagcaatta ttttatgtta cactttaaac aagtgaatta ttgaggagtt tttatgatag
      301 acgttgagaa tattcttgaa aaaatgaaac ctaaccaaaa aatcaattac gattgggtca
      361 tgcaacaaat ggttaagcag tggcaagcta gtgatatccg tccgaaaatt ctgatgcatg
      421 tttgctgtgc tccttgtagt acatataccc ttgaatactt atcacaatgg gcagatgtta
      481 ccatctattt tgctaattca aatatccatc ctaaagatga atattatcgc cgcgaatatg
      541 ttactcaaaa atttgttcat gatttcaaca aaaatactgg atattctgta caattcttat
      601 cagcaccata tgaaccaaat gaatttttca aaattgttca tgggttagaa gaagaacctg
      661 aaggtgggga tcgttgtaaa gtttgctatg atttccgtct tgataagaca gcggaaaaag
      721 ccgttgaact aggttttgat tactttggca gtgccctaac tattagtcct cacaaaaact
      781 ctcaaacaat caatactatt ggtattgatg tccaaaaaat ttatgatact cagtatcttc
      841 ctagcgacct taaaaaaaat aaaggttacc aacgttccgt tgagatgtgt aaagattatg
      901 atatctaccg acaatgttac tgtgggtgta tcttcggtgc taaagatcaa gggataaatc
      961 ttctccaaat caaaaaagat gcgaaagctt tcgtgagtga taaagacggc aaagaagaat
     1021 ttcctaatat acgcttcaca tttaatggca agtcaatgta aatgataaaa aaagacttag
     1081 taaagaacta agcctttttg gtataaatct tgtcaaactt taataataca acttcttaat
     1141 cttttgcaga cgttggttta gaaatagacc acaaacaaaa atacctgtga ttaaaccaat
     1201 ccaataggca aacggaccta actggctatt tttttctaag ataaccgcta atggcaaagc
     1261 acataaccaa taagagcccg caccgatcat aaatggtttt gttgtatcct tatagcctcg
     1321 taaaatcccc tgtacaggag ctgcataagc atctgcaaac tggaaaaaga gactataagt
     1381 taggaattga gctgtaatag cgacaaagtg agggtcacta ttatacattg ctgctacatt
     1441 ctcacgaaat agaaataaaa atagtaaggt tcctgatgta atccctaccg ctgttaagcg
     1501 tcctatccta ctataagtgt ttgcgtcctg aaagcgctct gcccctactt caaacgatat
     1561 tgtaatagct agagcagtgg aaatacttaa aggaaatgca tacattaatg atgaaaaatt
     1621 catagcagcc tgatgtgctg caataatgat tgaagaaaat tttgccatga ataagcctac
     1681 tactgcaaaa attgcaactt ctgcaaaaat ttgtaaacca atcggtaatc ccaatcgaat
     1741 atcttcaata atcaaaggag cttttattct ttccagagtc catatatgat atgttttaat
     1801 ttgaggatga agtgacatca caataataat aacaataaaa atagcccaat aagttaaaga
     1861 agttccaaga cctgcccccg cacctcctag tctaggcata ccaaatttac cgtagataag
     1921 catataatta aaaaatgaat taaagggtag aattaaaagc atcagataca tagataacct
     1981 tgttaacccc aatgcatcaa agaatgaacg gcaaatgcta aacaacacca gcggcatgat
     2041 tccaatcaac atataattta aataaccacg accaactgct agaacttcat cttctaaacc
     2101 caaactcccc aagacaggtt gagcaataaa ttgcatgatt aaaaataata ttaaggacag
     2161 tatcaaacct aaatatagaa attgatgaaa ttctgtgcga atttgttctt tatttcctct
     2221 acccaaatgt tgaccaacta ctggtactaa tgctgaaatc atacctacta ataaagcgaa
     2281 aaacggagtc cataaattac tagcagttga cacacctgcc aaatgtagct gactatactg
     2341 tccagtcata accgaatcaa taaaagtagc tgaaaaatta gcaaattgat aaatcaatac
     2401 tgggataaat aatttcaaaa ataaaacaaa tttttcctta tttgtctgag tttgatacat
     2461 cttttttctc cttttcacct taatcaattt attttagctt agctaggata ctctgtcaat
     2521 gctatttaaa aaggttgaga aataatcctc aaccccattt tagctaagct cagtattgat
     2581 ttgccatgtc ataccatatt tgtcagtcac atttccataa gctggactcc acggaactgc
     2641 ttgtagaggc attttaatgt tcattgcatc tcgagaaagc ttattgaata actcttgcgc
     2701 ttcttctaca gattccacaa ttaaagctgc tgtcatattt tgtccaattt gaaaatcaaa
     2761 aataggatta ttgtcagaaa gttgcattcg aataccatca atattaaatt gagcgttcat
     2821 aattaaatgt tcaagttctg gcggacaatc tggccaaccc tgtgcaaaag ttgtgacgtg
     2881 ttctgctttt gtattaaaag cctcttcata aaatgtaata gcttcggccc cacgaccatt
     2941 aatattcata taaatttcaa ttgctttcat taccatacct cacttcatat tatccaacca
     3001 acgctgcatc agagtttcat tttccccttt tagggtaaaa taagaaccac gtattatacc
     3061 tgcgagtttt gacttttgac gagcaaactt aaacttgtcc gctaattcaa taggaatatc
     3121 cattccatct gataattt
//
LOCUS       DATROY010000167         3109 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.168, whole genome shotgun sequence.
ACCESSION   DATROY010000167 DATROY010000000
VERSION     DATROY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.168"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..307
                     /gene="tilS"
                     /locus_tag="VBD11_001897"
     CDS             <1..307
                     /gene="tilS"
                     /locus_tag="VBD11_001897"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6954805.1"
                     /translation="LFSYGKFISQADITIPIYDTSPIILRRRKEGDRIFLGNHTKKIR
                     RLFIDEKITLKDREEAVIGEQNKELIFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            312..854
                     /gene="hpt"
                     /locus_tag="VBD11_001898"
     CDS             312..854
                     /gene="hpt"
                     /locus_tag="VBD11_001898"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6954806.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            877..2853
                     /gene="ftsH"
                     /locus_tag="VBD11_001899"
     CDS             877..2853
                     /gene="ftsH"
                     /locus_tag="VBD11_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6954807.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
BASE COUNT         1089 a          443 c          637 g          940 t
ORIGIN      
        1 tttattttcc tatggaaaat tcatttctca agcagacatt actattccta tttatgatac
       61 ttctccgatt attttaagaa gaagaaaaga aggggatagg atatttttag gaaatcacac
      121 taaaaaaatt cgccgtctat ttattgatga gaaaataact ctaaaagaca gagaagaagc
      181 tgttattggg gagcaaaata aagagttgat atttgtgatt gttgcaggta gaacatattt
      241 gagaaaacct tctgaacatg atataatgaa gggcaaactt tatattgaaa atttagaaaa
      301 aaggtgacat catgcttgaa aatgatataa aaaaagtttt atattcagaa gaagatatta
      361 ttctgaaaac gaaagaatta ggagctaaat taactgcaga ttatgcaggt aagaatccat
      421 tattagtagg tgtattaaag gggtcagtgc cttttatggc tgaactatta aagcatattg
      481 atacacatgt tgaaattgat tttatggttg tttcaagcta tcatggtggt acaactagta
      541 gtggagaagt taaaatttta aaagatgttg atactaacat tgaaggaaga gatgtaatct
      601 ttattgaaga catcattgat actggacgta cattaaaata ccttcgagat atgtttaagt
      661 atcgtcaagc taactctgtc aaagttgcaa ccctttttga taagccagaa gggcgattgg
      721 tagatattga tgctgattac gtttgttacg atatcccaaa tgaatttatt gtgggattcg
      781 gtttagacta tgctgaaaac tatcgtaacc taccttatgt cggtgtgtta aaagaagaaa
      841 tttactcaaa atagaaaaaa ggtactctct taaactatga aaaataataa aaataatggt
      901 tttctgaaaa attcctttat ttacatatta ttgattattg cggttattac aacctttcaa
      961 tactatttaa aaggaactag ctctcaaaac cagcaaattt cttatacaaa acttgttaaa
     1021 caattaaaag ctggagaaat taagtctatt tcataccagc caagtggtgg tgttgttgaa
     1081 gttagtggga catataagaa agccaaaaca attaaatcag ctaatagttt tactttttta
     1141 ggtggtagtg ttgctactaa agttacagga tttaattctg ttatactacc aaatgactct
     1201 tcaataaaat ctcttgttag cgctgctgaa gaaaataaca ctaatattca ggtcaaacat
     1261 gaaagttcaa gtgggacatg gatttcttat attgccagtt ttttaccttt agttatcatg
     1321 attggtttct ttatgatgat gatgaaccaa ggcggtggcg gtggcgctcg tggtgccatg
     1381 agctttggta aaaataaagc aagatcatct agtaaagatg aggttaaagt tcgattttcg
     1441 gatgttgctg gagcagagga agaaaagcaa gaattaatag aagtcgttga cttccttaaa
     1501 gatcctaaac gatataaatc tctaggtgcc cgaattccag caggggttct attagaagga
     1561 cctccaggga caggtaaaac cctattagca aaagctgttg ccggtgaagc aggggttcca
     1621 ttctttagta tttccggttc agattttgtt gaaatgtttg tcggtgttgg agctagtcga
     1681 gtacgctctt tgtttgaaga tgctaaaaaa gctgaacgtg ctattatttt cattgatgaa
     1741 attgatgctg ttggacgtcg acgtggagct ggtatgggtg gcggaaacga tgaacgtgaa
     1801 caaacgctca accaactttt aattgaaatg gatggttttg aaggaaacga aagtattatc
     1861 gttatcgctg ctaccaaccg tagtgacgtg ttagatcccg cattactgcg tccaggacgt
     1921 tttgatagaa aagttctagt tggacaacct gatgtaaaag gtcgtgaagc aattctgaga
     1981 gtacatgcta aaaataaacc attagcagat aatgttgact taaaagtagt agctcaacaa
     2041 accccaggat ttgttggtgc tgatttggag aatgttctca atgaagctgc tttagttgct
     2101 gcgcgacgaa ataaaaaagt tattgatgct tcagatattg atgaagcaga agaccgtgta
     2161 attgctggtc cttctaaaaa agatcgtact atttctgaaa gagaaagagc tatggttgct
     2221 taccatgaag ctgggcacac aattgtcggt ttgattcttt caaatgcgcg agttgttcat
     2281 aaagttacta tcgtaccacg tggtcgtgca ggtggatata tgattgcttt acctaaagaa
     2341 gatcaaatgc ttctttcaaa agatgatatg aaagagcaat tagcaggtct tatgggagga
     2401 cgtgtagcgg aagaaattat tttcaacgct cagacaacag gtgcctcaaa tgactttgaa
     2461 caagccactg ccatggcccg cgctatggta actgaatatg gtatgagtga gaaacttggc
     2521 ccagttcaat acgaaggtaa ccatgcaatg atggctggcc aaatgtcacc tgaaaaatca
     2581 tattctgctc aaactgcaca acttattgat gatgaagttc gtcaccttct taatgaagca
     2641 cgtaataagg cagctgatat tatcaacgaa aaccgtgata ctcataaatt aattgcagaa
     2701 gccctcttaa aatatgagac tcttgatgct gctcagatta aatcgatttt cgagacaggt
     2761 aaaatgcctg agacagaaaa tgatgaagat aaagcgagag cgttatcata cgatgaaatc
     2821 aaagaaaaaa tgcaagaaga agataaaaaa taagtcatca tatattttta aagaacttta
     2881 taaatttcaa acacccttca aatttagata aaatttgagg ggtgtttttt atgaagatga
     2941 agtttaaatt tgtgaattaa gaagatgaaa tatgaacatg acaacggtaa aaatgctatc
     3001 cattaaacaa gtatagaagg ttagttacta taaataatat taacttttta aaaataaaag
     3061 aaaaaatgca aaaatagtat tgacaggaag tagaaatatt tagtataat
//
LOCUS       DATROY010000168         3101 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.169, whole genome shotgun sequence.
ACCESSION   DATROY010000168 DATROY010000000
VERSION     DATROY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(63..2054)
                     /gene="uvrB"
                     /locus_tag="VBD11_001900"
     CDS             complement(63..2054)
                     /gene="uvrB"
                     /locus_tag="VBD11_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6954808.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(2115..3035)
                     /locus_tag="VBD11_001901"
     CDS             complement(2115..3035)
                     /locus_tag="VBD11_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6954809.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
BASE COUNT         1025 a          638 c          473 g          965 t
ORIGIN      
        1 cttatttaat aatctataac cttctgctat ctcctaaaga gagaaccaga tgagagataa
       61 gtttaatcga ttgcttttag ttctaagata acatctcgga tttgtgcagc tagctcaaag
      121 tccaataatt cagctgcatc ttgcatttgc ttttgaagag ctttaatttt agcttgcctt
      181 tcttttttag ataaactact gtaatcgaca actttttcag gcttatcgct atcattactt
      241 ttagtaatag caattaaatc acggatttct ttttttattg tttgtggcac aattccatgc
      301 ttttcattat aatccatttg taaacgacga cggcgagcgg tctcatccat agctctttgc
      361 atagaatctg ttattttatc agcatacata ataacatgac cattactgtt acgtgctgca
      421 cgtccgattg tttggataag accacgctca ttacgaagga aaccttcctt atcagcatct
      481 aagattgcaa caagggaaac ttcaggaaca tctattcctt cacgtaagag attaatacca
      541 attaaaacat caaacacacc aagtcgtaga tcacgaataa tctctgtacg ttctaaggtt
      601 ttaatatcgc tgtgcatata cttaacttta actcccattt ctttgagata atctgttaag
      661 tcttctgcca tacgctttgt caatgttgta ataaaggtac gctcaccttt ttcagtcctc
      721 aaattgattt caccaagcaa gtcatccatc tgccccatac ttggtctaac ttcaacttct
      781 ggatcaagaa gtcctgttgg ccggataatt tgctcaacaa ctgtatctgt ttgctccatt
      841 tcatagtctc ctggcgttgc agatacatag acaatttgat ggacatggct ttcgaattct
      901 tcacgacgaa gtggacggtt atctaaggca gatggtaaac gaaaaccgta attaaccaac
      961 atttctttac gcgaacggtc tccattatac atccctttga tttgccccat tgtcatgtga
     1021 ctctcatcaa tcataatcaa aaaatcttct gggaagaaat ctaacaaagt aaaaggtggc
     1081 tcaccttcac ttctaccatc catgtgacgt gagtagttct caacaccatt tgtatatccc
     1141 atttcacgaa gcatttcaat atcatattct gtcctttgtc gaatacgttg cgcttcaatc
     1201 aatttccctt ctttttcaaa aagttctact tgattttcca tttcagcttg tatttttgaa
     1261 atagcttctt ccatatgttc atcatttgtc ataaagtgtg ttgctgggaa aatagctaaa
     1321 tgttccactt ctcctagcac acgccctgtt aacgattcta tttctcttat acgatcaatt
     1381 tcatcaccaa aaaattcaat tcgaaaggca tgctcatcac gactcgcagg aaaaaattct
     1441 acaacatcgc cacgaacgcg aaattttcca cgttgaaaat caatatcgtt acgctcaaat
     1501 tgaatatcaa cgagattgtt caataactga tcacgtgaaa tttcttgacc tggcctaagg
     1561 ctaacaacac tgtctgcata ctctttagga gaacctaaac cgtaaataca agaaacagat
     1621 gctacaacaa taacgtcatt tctttctaat aacgaagaag tcgctgagtg acgtaatttg
     1681 tcaatttcgt cattgactga gctatctttt tcaatataag tatccgaaga aggcacataa
     1741 gcttctggtt gataataatc ataatatgaa acaaagtact ctacggcatt gtcaggaaaa
     1801 aattctttaa actctccata aagttgacct gctagggttt tattatgtgc aatcaccaag
     1861 gttggcttgt taacttgggc gataacttgg ctcattgtgt aggttttacc agtaccagta
     1921 gcacctttta aaatctgagc tttttctcct ccttcaatat tgtcaaccaa cgtttcaata
     1981 gcttgaggtt gatctcctga aggactatat ttggaaacta atttaaatcg gttagtatct
     2041 tttctatcta tcatttcttt acctcgatta ctttatttta acatagaaaa aagaaaaaga
     2101 ctgagctgca acactcaatc tgtttcttca gtcttaatta gccatagaag gtatataatt
     2161 gcagctaaga gaacaaaaat agaaatcaaa cttccttcta ttccgaatgc accacctgat
     2221 atccagtccg gtgcgttttc tttaacggta aaatgcaaaa gagatgctcc tgctttctga
     2281 ccacttactg caataccata gagattacct tggctaaaat tccaagctcc atgtaaggct
     2341 gcaacgctcc aaatattacc acttttaatc atatacaagg acattaaaac tcccgaacat
     2401 ataatactta caatagaaag aaaagtgaca tgggcattta ccaagtgcaa aatgccaaat
     2461 aaagtggatg agacccctat ggcaacagct aaatgaaaac gatgattaat tagaggaaga
     2521 agccaccctc gtgttagcaa ttcttccgta ccgctttgaa taagccaaaa aggaaggaga
     2581 gttacaacat agaaaatcgt tgccttagaa acatcatagc tatgaaagtc aagtcctccc
     2641 aatatataag cagtgatgac agaaattgaa aatagaataa aacctatact ccagcctttt
     2701 ataagtgacc acagagttct tttaaacttt gtagaaaaac ccaattctga aaaaggtctt
     2761 ttttctacga attttaccca gataagcaaa aggaatgctg aaaaactaaa ggttcctaat
     2821 tctccataga tattattaaa taaattaata aactcaaaaa aagtatagga agaaaactta
     2881 tagagtacag aagacactag aacaattccc acaccaccta gagttagacc gacgaaaaaa
     2941 aataggtata ccataaaaca agcaaataac aataatagcc atgacggcca gtttttatag
     3001 acagcgaact gactatttaa aatcctttta ttcataataa tcctaaatcc tttttgtgtg
     3061 atgacactag aacaattata tcataatact aactaaagat a
//
LOCUS       DATROY010000169         3060 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.170, whole genome shotgun sequence.
ACCESSION   DATROY010000169 DATROY010000000
VERSION     DATROY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.170"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..252
                     /locus_tag="VBD11_001902"
     CDS             <1..252
                     /locus_tag="VBD11_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6954810.1"
                     /translation="GKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIWA
                     LGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            321..1419
                     /gene="prfB"
                     /locus_tag="VBD11_001903"
     CDS             join(321..392,394..1419)
                     /gene="prfB"
                     /locus_tag="VBD11_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6954811.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            1438..2130
                     /gene="ftsE"
                     /locus_tag="VBD11_001904"
     CDS             1438..2130
                     /gene="ftsE"
                     /locus_tag="VBD11_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6954812.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            2114..3043
                     /locus_tag="VBD11_001905"
     CDS             2114..3043
                     /locus_tag="VBD11_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954813.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
BASE COUNT         1002 a          468 c          642 g          948 t
ORIGIN      
        1 ggtaaaaata ttttacagcg taatgccatt attgcgctag caaatgctca tgataaaacc
       61 gcagtggtta aattaataga gattattgat aaaaataata accctattca tactgcaaca
      121 gctatatggg cattgggaga aattgtcaaa aaaccaaacg atgagatact ggagtttatg
      181 agtaatttaa ccttaaaaga tgaagatagc cgaaaagagt tggaattaat ccgtcataaa
      241 tggcaatttt aagcctttta tggtattttt aagaacctta tgataaaata aaacagatta
      301 acaaagattg gagaaatctc atggaagtgg ctgaaattcg ccaaaaaata gtagaaaata
      361 aagagaagtt gactagcttc aggaggtctc tttgacttag atcgtttgga agaggagatt
      421 gctcttcttg aaaatcaaat gactgagcca gacttttgga atgacaatat tgcagcgcaa
      481 aagacatctc aagaactgaa tgagttaaaa ggtaagtatg atactttcca taatatgcaa
      541 gaattatctg acgaaacaga actcttactt gagatgcttg acgaagatga ttctttgaaa
      601 gaagaattag aagaaaatct aatgcagcta gataagataa tgggtgctta tgaaatgacg
      661 cttctcttat ctgagcctta tgaccacaat aatgctatct tagaaatcca tcctggttca
      721 ggaggaactg aagcgcaaga ttggggagat ttactcttac gaatgtatac ccgttttggc
      781 aatgctaatg gtttcaaagt agaagtattg gattatcaag caggggatga agctggtatt
      841 aaatcggtta ccctctcttt tgaaggtccc aatgcttatg gtttgttgaa atcagaaatg
      901 ggagttcatc gcctcgttcg tatatcacct tttgattcgg ctaaacgtcg tcatacctca
      961 tttgcttcag ttgaagtgat gccagagtta gatgatacga ttgaagtaga agtacgtgat
     1021 gatgacatta aaatggatac tttccgttcc ggaggagctg gtggtcagaa cgtcaataaa
     1081 gtatcaacag gtgttcgttt gacgcatatt ccaactggaa ttgtggtatc gtctaccgtt
     1141 gaccgtactc aatatgggaa ccgcgatcgc gctatgaaga tgttgcaggc taagctatat
     1201 cagttagaac aagaaaagaa agcacaagaa gtagatgctc tcaagggtga taaaaaagaa
     1261 atcacttggg gaagtcagat tcgttcttat gttttcaccc cttatacgat ggtgaaggat
     1321 catcgtacta actttgagct tgctcaggta gataaggtta tggatggaga aatcaatggc
     1381 tttattgatg cctaccttaa gtggcgcatt gaagactaaa aataatgaag gagaaacatg
     1441 gcactaatag aaatgtctgg tgttactaaa aagtatcgcc gttcaacaac tgccttgaga
     1501 aacttaaatt tatcaattca gcaaggtgag tttgtgtatt tagttggtcc gtcaggtgct
     1561 ggtaaatcta gcttgattcg cctcctatac cgtgaagaaa aattaagttc aggtagatta
     1621 aaggtagggg aattcaatct caataaactc aaacgtcgtc aaattcctat tttacgtcgt
     1681 agtattggag ttgtcttcca agattataaa ttactaccaa caaaaacagt ttatgagaat
     1741 gttgcgtttg cgatgcaagt tattggagct aagcgccgtc atattaaaaa acgcgtgcca
     1801 gaagttttag aattagtagg tttaaaacac aagatgcgtt cttttccaac acagttatct
     1861 ggtggagaac aacaacgtgt ggcaattgca cgtgcgattg ttaataatcc aaaattatta
     1921 attgcagatg aacctactgg taatttggat ccagaaatcg cttgggaaat catgcatcta
     1981 ttggaacgta ttaaccttca aggaacgact gttttgatgg caacacacaa tagtcaaatt
     2041 gttaacacac ttcgtcatcg tgtcattgag attgaagcag gtagcgttat ccgagatgaa
     2101 gagaagggag aatatggtta tcatgattaa tttctttaga catttctggg aatcgcttaa
     2161 aaacttaaaa cgtaactttt ggatgacttt tgcttctgta acatcagtta ccataacatt
     2221 acttcttgta ggcttgtttt catcagtttt actgaatgtt gaaaagttaa caactgatgt
     2281 ttcaggtaat tttactatca gtgccttttt aaatgttgac tcaacggatg ctcaaaaaca
     2341 agtcaaagat aaggacggaa agctaaaaga taatcctgat tatcataaag tttatgacaa
     2401 gattaaacgt atttctggtg tagagaaagt cacttattca agtaaagctg aacaattaaa
     2461 agaagttcaa aaagagtatg gttcagatgt tattgatgat acctacaaag atgctttgtt
     2521 agatgtttac gtagttggga caagttctgc aaaagtttct aagagtgtca gtgaagcaat
     2581 tggacgcatt gaaggtgttg attatactaa agaacctatt gattcaacaa aactatctaa
     2641 tctcacagat aacatccgta tatggggatt tggtggtgtt gccttattaa ttgtccttgc
     2701 tatctttttg atttcaaata ctattcgtat gtcgattatg tcacgccgta cagatattga
     2761 aatcatgaga ttagttggcg ctaaaaattc ttatattcga ggaccgttct tttttgaagg
     2821 tgcttgggta gggattttag gggctatcgt tccatcgctg attttctatt ttggttatca
     2881 atttgtgttt aacaagttta atccaaaatt tgagacaagc catgtttcat tgtatcctat
     2941 ggatattatg gttccagcaa ttattggtgg tatggtgatt attggtatta ttattggctc
     3001 acttggatct gtcctatcaa tgagacgtta ccttaagatt taataaaaag agatgaaatc
//
LOCUS       DATROY010000170         3034 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.171, whole genome shotgun sequence.
ACCESSION   DATROY010000170 DATROY010000000
VERSION     DATROY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.171"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            93..728
                     /locus_tag="VBD11_001906"
     CDS             93..728
                     /locus_tag="VBD11_001906"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6954814.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            748..1611
                     /locus_tag="VBD11_001907"
     CDS             748..1611
                     /locus_tag="VBD11_001907"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6954815.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            1641..1967
                     /gene="yabA"
                     /locus_tag="VBD11_001908"
     CDS             1641..1967
                     /gene="yabA"
                     /locus_tag="VBD11_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6954816.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            1967..2836
                     /gene="rsmI"
                     /locus_tag="VBD11_001909"
     CDS             1967..2836
                     /gene="rsmI"
                     /locus_tag="VBD11_001909"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6954817.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
BASE COUNT         1051 a          478 c          587 g          918 t
ORIGIN      
        1 ttaaaaatat cagacatgca caatcaagtt gtgggtgtct ttttttgata aattttgata
       61 gaatagtagt aattataaat aaaggatgta ccatgaaaaa aggactaatg atttcttttg
      121 agggcccaga tggtgcgggt aaaacaaccg ttttagaagc tgttttaccc ttgctcagag
      181 aaaaattatc acaagatatt ctcaccacca gagagcctgg tggcgtaact atttcagaag
      241 aaattcgtca tattatttta gatgttaaac acacgcaaat ggataaaaaa acagagctat
      301 tgctttacat ggctgctcga cgccaacatc tagtggaaaa ggtactccca gctctagaag
      361 aagggaaaat agtcctaatg gatcgtttta ttgatagttc tgttgcctat cagggatcag
      421 gaagaggatt agataagtca catataaaat ggcttaatga ctatgcgact gatagtcata
      481 aacctgattt aactttatat tttgatgttc cttctgaagt aggtttagag cgtattcaaa
      541 aaagtgttca aagggaggta aatcgtctag atctcgagca attagatatg catcaacgcg
      601 tccgtcaagg ttaccttgaa ttggcggatt cggagccaaa ccgcattgtg acgattgatg
      661 cctcccaaca gctagatgaa gtaatagctg agacattctc tattatttta gatcgtatca
      721 accaatgaat caggaagtac aaaggtcatg gatttaaagc gaacacaacc caaattgctg
      781 gaaaagttta atactatttt acagtcagat aggatgagtc atgcctatct tttttcggga
      841 aattttgcaa gtctagatat ggcactttat ttagcacaaa gtcagttctg tgaaaagcgc
      901 cagagtggtt taccatgtca agagtgtaga gcctgtcgtt taatcgctaa tggggaattt
      961 tcagatgtta aaatcataga accacagggg caacttatta agatagagac tataaaagag
     1021 ctgactaaag atttttcaag atcaggtttc gaagggaaat cacaggtctt tatcatcaaa
     1081 tattgcgaaa aaatgcatgt caatgcagcc aacagtcttt tgaaatttat tgaagaacct
     1141 caaagttcat cttatgtcat cttattgact aatgatgaaa acaatgtttt gccaacaatt
     1201 aagagtagaa cgcaaatttt tcgttttcca aaacaattgg acatgttggt tcatcaagct
     1261 gaacaagcag gattgctaaa atcacaagct agcttattag ctcaagtagc agacgatcct
     1321 aaacatttag aaatattgtt gactaacaaa aagttattag attacttgaa tttaagtcaa
     1381 cagtttgtga cgactttggc aaaagatagg caaactgctt atttagaagt atctcgcttg
     1441 acatcgcaag ttgtagataa aaatgatcag gcatttgttt ttcaatggct gacaataatg
     1501 ctggctaaag aaggccaact ttatgattta gaaaatactt atagagctca gcaaatgtgg
     1561 aagagtaatg ttagttttca aaatagttta gagtatatgg tgctttctta gaaaataata
     1621 cgcgcagaga ggaagagtga atggataaaa aagacttgtt tgatgccttt gatgattttt
     1681 cacaaaattt attagttggg ttgtctgaaa ttgagactat gaaaaaacaa atccaaaaat
     1741 tattggagga gaatactgtt ctacgcattg aaaatggtaa acttcgtgag cgtctgagcg
     1801 ttattgaagc agaaacagaa actgcagtta aaaattctaa acaaggaaga gaattactcg
     1861 aaggcattta caatgatggc tttcacattt gtaatacttt ctatggtcaa cgtcgtgaaa
     1921 atgacgaaga atgtgctttt tgtattgaat tattatatag agattgatgg aaatgcaagt
     1981 tcaaaaaagt tttaaatcaa atatacatta cggaacactc tatctagtcc caactccaat
     2041 tggtaatcta gatgatatga cttttcgtgc cattaggatt ttaagagaag ttgattttat
     2101 ttgtgcagag gatacacgaa atacgggact tttactcaag cactttgata ttactactaa
     2161 acaaattagt tttcacgaac acaatgctta cgataaaatc tctgggttaa ttgatttgtt
     2221 aaaagaaggg aaatctttag cccaagtatc tgatgcagga atgccctcta tttctgaccc
     2281 aggacatgac cttgtcaagg ctgctattga aggggatatc ccagttgtat ctataccagg
     2341 agctagcgct ggtattactg ctctcatcgc ttcaggttta gctccacaac ctcatatttt
     2401 ttatggcttc ttaccacgta agaaaggtca acaaataact ttctttgaaa caaagcaaga
     2461 ttaccctgaa acacaaatct tttatgagtc accgtttcga gtctctgata cgctaaaaca
     2521 catgaaagag atttacggag atcgccaagt tgttttagta cgcgaattga cgaaactcta
     2581 tgaagagtat caaagaggaa ccattagtca acttttagag catattgaaa aggtccctct
     2641 caaaggtgaa tgcttaatta ttgttgatgg taagagagat accgagcgag tgaaagacag
     2701 tagccaacaa gatccactag tattagtaaa agaatatatc gctaatggtg ataaaactaa
     2761 tcaagcgata aaaaaagtag caaaagaatt taatctcaat agacaagaac tctatgctag
     2821 tttccatgat ttataagtaa taatataaaa aggttgccaa agaaaaattt ggcaaccttt
     2881 tataatctcc gatttagcta ttggaataaa gaagtaagat acttccaatt tccttaaaat
     2941 ttttaatttc agctcttttg aataccattg caaattggat tttatttctg agagtaggtt
     3001 ataaaagatg gtatgctctt attccggttc tttc
//
LOCUS       DATROY010000171         2602 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.172, whole genome shotgun sequence.
ACCESSION   DATROY010000171 DATROY010000000
VERSION     DATROY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..903
                     /locus_tag="VBD11_001910"
     CDS             <1..903
                     /locus_tag="VBD11_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6954818.1"
                     /translation="TAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSDDELREIAK
                     LVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYINKRDGYNF
                     KLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAEILVYGASV
                     IHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTHIFINNDIN
                     MMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQDQAFLFDER
                     IRKLHPKGRGLDTGFYDFDPSTVK"
     gene            1033..2319
                     /locus_tag="VBD11_001911"
     CDS             1033..2319
                     /locus_tag="VBD11_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6954819.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            2446..>2602
                     /locus_tag="VBD11_001912"
     CDS             2446..>2602
                     /locus_tag="VBD11_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954820.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGK"
BASE COUNT          849 a          388 c          555 g          810 t
ORIGIN      
        1 acagctgaat ctattgaaca agtaaagcaa ttgctagcaa taggaattga ccgcatctac
       61 gtaggggaag aaaattacgg tcttcgttta ccacattcat tttcagacga tgaattgcgt
      121 gaaatcgcta aattggtaca tgatgctggt aaagaattga cagtagcttg taatgcactc
      181 atgcatcaag aaatgatgga taatatcaaa ccatttttgg agttgatgaa agaaattaat
      241 gtggattatt tggtagttgg tgatgcagga gtattttata tcaacaagcg tgatggttat
      301 aacttcaaac ttatctacga cacttctgta ttcgttacat caagtcgtca agttaatttt
      361 tggggacaac atggagcagt agaaacggtt ttagcacgtg aaataccttc tgaagaactt
      421 tttaagatgt ctgagaacct tgaatttcct gctgagattt tagtttatgg ggcttctgta
      481 attcatcact ctaagcgtcc gttattgcaa aactattata attttactca tatcactgat
      541 gaaaaaacac gtgagcgtgg tcttttttta gcagaaccag gtgatccaga aagtcactat
      601 tctatttacg aagataagca tgggactcat atttttatta acaatgatat caatatgatg
      661 actaaagtga ctgaactagt tgaacatcac tttactcact ggaaattaga tggtatttat
      721 tgtccaggag ataattttgt tgctattgct gaaatttttg tcgaaacagc tcgtttgatt
      781 gaaaatggaa cattcactca ggatcaggct ttcttgtttg atgaacgaat tagaaaactg
      841 caccctaaag gacgtggttt agatactgga ttttatgatt ttgacccatc aacagtaaaa
      901 taagatttat ttgctacgtg aaagtttttt atctcaaagc gtagccaatt gcactaagaa
      961 ctgcatatcg attggtatgt taattaagat agaaaataat tcttttatct tgaaaataga
     1021 aggaaatata agatgtctaa tgtaaaaaaa cgccctgagg ttttatcacc tgcaggaaca
     1081 ctagaaaaat taaaagttgc tattgattat ggagcagatg ctgtatttgt tggaggtcaa
     1141 gcgtatggtc ttcgaagtag agctggtaac ttttctatgg aagagttaca agagggaata
     1201 aactatgctc atgcgagaga tgctaaagtt tatgtagcgg ctaatatggt tactcatgaa
     1261 ggtaatgagc ttggggcagg tccgtggttt cgtgaattac gtgatatggg actagatgca
     1321 gtcattgttt cagatcctgc tcttattgtt atttgtgcta cagaagcacc aggtttagaa
     1381 attcatttgt caactcaagc ctcttccacg aactatgaaa cttttgaatt ttggaaagag
     1441 atggggctta ctcgtgtcgt attagcacgt gaggttacta tggcagagtt ggctgaaatc
     1501 aggaagagga cagatgttga gatagaagca tttgttcatg gcgcgatgtg tatttcatac
     1561 tcaggacgat gtgttctatc aaaccatatg agccatcgtg atgctaatcg tggcggttgc
     1621 tctcagtcat gtcgttggaa atatgacctc tacgatatgc catttggaca agaacgtcaa
     1681 tcgttaaaag gcgagattcc agaacctttc tcaatgtcag ctgtggatat gtgtatgatt
     1741 gagcatattc cagatatgat tgaaaatggc gtagatagtt taaaaataga aggacgtatg
     1801 aaatccattc attatgtttc tacagtaact aattgctata aagctgctgt agatgcctat
     1861 atggaaagtc cagaagcttt tgaagctatt aaagaagact tgattgatga actttggaag
     1921 gttgcacaac gcgaattagc aacaggtttt tactaccata caccaactga aaacgaacaa
     1981 ctctttggag ctcgtcgtaa aattcctcaa tacaaatttg ttggggaagt ggtttcattt
     2041 gacaatgcta aaatggaggc tacaattcgt cagcgtaatg ttattatgga aggagatcgc
     2101 gtagaattct atggtcctgg cttccgtcac tttgaatgtt ttattgaagg tctgcgcgat
     2161 gctgaaggaa ataaaataga ccgtgctcca aatccgatgg aattattaac cataacatta
     2221 ccaaatccag taaaaaaagg ggatatgatt cgtgcttgta aagaaggatt agtgaacctt
     2281 tatcaaaatg atggtactag caagactata agagcttaga tacttttata actattttgt
     2341 cattttacat ggtcaatatt actaaaaatt gttaaaatag aagaagttca tttaaataga
     2401 acaaaaagct ataaaaataa cgaatagtat aagtggaggt ataaaatgtc agaatttaca
     2461 tttgaaattg ttgagaagtt attggtccta tctgaaaatg aaaaaggttg gacaaaagag
     2521 ttgaatcgtg ttagtttcaa cggagcgcca gctaaatttg atttgcgtac atggagtcca
     2581 gatcatacaa aaatgggtaa ag
//
LOCUS       DATROY010000172         2592 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.173, whole genome shotgun sequence.
ACCESSION   DATROY010000172 DATROY010000000
VERSION     DATROY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.173"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            142..672
                     /locus_tag="VBD11_001913"
     CDS             142..672
                     /locus_tag="VBD11_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954821.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQINGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            764..987
                     /locus_tag="VBD11_001914"
                     /pseudo
     CDS             764..987
                     /locus_tag="VBD11_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1319..1795
                     /locus_tag="VBD11_001915"
     CDS             1319..1795
                     /locus_tag="VBD11_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954822.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            2051..2388
                     /locus_tag="VBD11_001916"
                     /pseudo
     CDS             2051..2388
                     /locus_tag="VBD11_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          965 a          343 c          487 g          797 t
ORIGIN      
        1 aaaaaatcct aaaaaatcat ctatttcggt tacaaaaacg gaaagtaaag aatattatta
       61 tacaatcagt attgatactg attctgagat atattctata ttcgaaggta ttacattaga
      121 ggatgtaaag gagttttcta aatgagtcca agtgagaaat tagatagaag aattgattct
      181 tcatcagacc tctcccagta tgtatatgat tttgaaaagc aatatacaga atatatgaag
      241 aaggggtatt ctgaaaaaaa tgcgagtata caagcacgac gaaccctgaa tgaaatacta
      301 caagaaaata atccaggatt gagatctagc gacgccatgt atgataaaga aacaggtatt
      361 gcagctattg ctgtaaaaga taaaaatact ggtgagactt atatagctta tgcgggtact
      421 aataaggatg ctgatggggc aaccgacttt atttcagatg caaatatcgg tgctaataac
      481 tctgcttatt taaaacgatt aggagacaaa gctactagct tttatgatcg tgttcaaatt
      541 aatggcgata atattactgt cacaacaggt cattcttatg gtgactttct cgcttctaga
      601 gtggcaattg agctaattcc attctttatt aactttagaa aagttaatga ttcatttcac
      661 ttttgttatt agcttgtgat cacttataaa gtgacagcta gttcatgaaa tttcttagga
      721 gttttctggc tttaaaggta acctatgatt taaaaaacat ctcatggaat tcaatgcgga
      781 atcctcaaca gtcttttgtg gactaacaga agttcaggat gtcgtttcgg caggccttaa
      841 ctattctcag aaggttttgg aaatttcaat ggtacatttg atacctcagg taagggatta
      901 gaatggacaa aaacaattca aacaggtatg aaaaaacgag aagagaaaat caaataccct
      961 cattttacaa ttgttgacat aaagtaatta aacaaaatcc ggaaaattta tattaagtaa
     1021 tttttctaaa aagtcatata ggcatacttt taaagttcct ctaaacttaa taaggaagaa
     1081 acgttttgaa aaaagataaa tatattgcat tgaattggtt gtgtgcgata gccacgctat
     1141 actattaagt tattacaaac tatgaattac acctcaaatg ttggagataa aacctatcgt
     1201 ttggggtgtt ttttggtaaa atagaatgaa aagagttact ctgtgtcatt actaagttaa
     1261 aaaattatac taacttaatt attaacgttt tattgactga gttactcttt tttaaaaaat
     1321 gtatatcatt ccagctggtt actcacctga acgtttagac tcagaagtcc ttacagcaaa
     1381 gattgaagat gatgaagcct tattgcggaa aattcaagac cttaaagatc atttagataa
     1441 aaataccaaa atcttggctt acgcttttga tggcttggaa gtcagagtga aagctagact
     1501 aagaaaaaat cgtaagaaac tcaaaaacct tatagaattc aatgccaaat ctacctcagt
     1561 ctttaatgaa ttgaaggatt attcaaatgc tgtatcgtct ggtttaacct ttgtatctaa
     1621 aggctttagt agtttcaatg gccagttctc ttcagcctca aatcttgatt ggcaaaagac
     1681 tattaagaca ggaatgagaa aacgagaaga agatgctaaa cagttgagaa aaaaagcttt
     1741 agaagattca aataagctca gaaaaattgc atcaaaaagg ggttactttg aataatgcag
     1801 ttgaatttgc taaagaacag aacaaacccc taaaaataaa aagataacca tagataaata
     1861 tggtcgtgta aaaatggata aaacttttct ttatcgaaaa gctaaagatg gaaaatctgc
     1921 atatatttat tctgaaggga gtgatcaata taaagaacta actgggaaag aatttgatcc
     1981 taaaactaca aatattatga aaggtagaaa attagggaca gatggtaaga tatcaattga
     2041 ttttggtgag attagtaact caggaaaaaa tgcagctatt aagtccctta aaaatgaaag
     2101 tccttgaatt aaatcccaaa aatgctttta aaggatggaa tgagttaagc aaaatagcta
     2161 aaactgggaa agtactagga tggacaggta aaattgctaa tatttacatg aatggaaaaa
     2221 aagatctttt tgatgataaa acatcttcaa tagatcaaaa aatcaaaaac tttgctatag
     2281 gttcaggtgt agatgcattg tccggtgcag caatagggac tttggtagga ggacctgtgg
     2341 gaaccgttgt tggagcaggt gcagggctgg catttgattt tgctttgaat tttaaagtta
     2401 aggagttggg taatgaatca attacaggtt ttgctaaaaa aggtttaaaa aatcttgcag
     2461 gaggattagg attttgaaaa aaagtataaa tgtaagtagg tatcatggac ctttaattga
     2521 atttgaggtt gatgcaagtt atttaagaat tttttcatat atcggtttac ctttatcatt
     2581 gttttggtat tt
//
LOCUS       DATROY010000173         2523 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.174, whole genome shotgun sequence.
ACCESSION   DATROY010000173 DATROY010000000
VERSION     DATROY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            8..76
                     /gene="shp2"
                     /locus_tag="VBD11_001917"
     CDS             8..76
                     /gene="shp2"
                     /locus_tag="VBD11_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6954823.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            137..1726
                     /locus_tag="VBD11_001918"
     CDS             137..1726
                     /locus_tag="VBD11_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6954824.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            1762..1938
                     /locus_tag="VBD11_001919"
     CDS             1762..1938
                     /locus_tag="VBD11_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954825.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(1989..2183)
                     /locus_tag="VBD11_001920"
     CDS             complement(1989..2183)
                     /locus_tag="VBD11_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954826.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            2182..2376
                     /locus_tag="VBD11_001921"
     CDS             2182..2376
                     /locus_tag="VBD11_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954827.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
BASE COUNT          913 a          463 c          414 g          733 t
ORIGIN      
        1 gatagtcatg aaaaaaatta acaaagcttt gttgttcaca ttaatcatgg atatcctaat
       61 tatcgttggt ggataaaact ccccacaaat ggttgttatg gaatcccatt ttatttagtt
      121 tataacagga gaactaatga agattaaaaa aattattagt ggctttgccg cagctttaat
      181 tatcagttca ctatcaacta ttaactatga ggttaaagct gatgacacca ccagtgagta
      241 tcactatatc agtaagcaaa ataatgaaaa gcagcttatt agttacatca aggaacaaca
      301 tcgtttgctc aatcaatttg ttgttgataa tgtcaactca ttcactcaac taaatgctaa
      361 tccaactatt gaacagttaa atagagctat aacattattt aaacaaaaag atgagcaatt
      421 atttaaccag gtgaaagctg gtcatctctc tcccagtaac tataatgcta tcgttaatca
      481 acgtaatgtc attaaccaaa ctgttcaaaa tctgattgac caaaatcata ataagattca
      541 gacaagtcaa aataaagcag ctcagctcgt ggggcaacgt aatcaggttg ttaacaaaat
      601 tcaagctatt ttagcaactg taaactacaa ctctgtgaat tctatacaag aagctgaaaa
      661 tttatttcat tcactcagaa atcaaattga acctcttgta gctgaagtta ataattacaa
      721 agctgctatg gcaatccttc aacaagaagt agatgcccta tcaacagcgg ctattgaaac
      781 tgagacttct aaacttgcta ctctcaaagt tagcgaaaat acttctgttc ctgcaaacaa
      841 agtagaagaa aaaactactc aatcagaagc gtcaggcaat aaacaagaag taactaagag
      901 tgaggaaaaa caggctacct ctgatgcaaa ggcatcacag cctgagtcag ctaatattgc
      961 cgattacgat agtttaaaag aagttttacg aaataatatt agcaaccaag taccacacat
     1021 cagtgttcaa atggagttta aaactcaaga acaagttgac gaataccaaa aaaatctcgg
     1081 aagcatcatc cgggaaattg gagatacact tggaacagca actgaattca atgccaaaag
     1141 taacattagc acttatactc ttggtggaca aatccaacgc attattgtaa aaagcgacat
     1201 cacaatcacc tatactctta aaggtgacat ggtaggatta cataaagaat ataaacagtt
     1261 tgtagattct tttgtcaaag aaaatattac taacaaaaat atcacaagtg attatgaaaa
     1321 agctaaagta attcatgacc acttggttaa taattacact tacgcgactg aagaactggc
     1381 aaccactcgt gaaactgcta gtggtatcag tatccatgct cctgaagcac tctacaaaga
     1441 taaacgtggt gtttgtcaag cctttgcagt aatgtttaaa gatatggctg ctgctggctt
     1501 atcagtatgg tatgtaactg gtcaagctgg aggtggaaat cacgcttgga acattgttac
     1561 tattaatggc gttaaatatt atgttgatac aacatgggat aataatataa aaagcaataa
     1621 atatttcctt gttggtaaaa caataatgga tgctgatcat cttttggata gtcaatacaa
     1681 tgcattagct aaagatattc cagctgatcg ccatcaaggt gcataactca aaaattgtat
     1741 tgagcctgga aaggatatga aatgtctaaa aagactgatt caaattatct acattccaat
     1801 tgttctgaca agaaaatttg gagcgatgag gcgcctctgc aacaggagac tatccctaaa
     1861 gacagagatt gtgttatcaa acctgtcatt actatgacag ttgaacatgt ggaaatccca
     1921 ttagatgacc aatcttagct ctctcctaaa ataaaaagga acactgccat ttttgacagt
     1981 gttccttttt atttaaccaa agtggttttc tcattactga tatgattcaa atcatctaca
     2041 actaattgat caatcgattt catggtgtat ttccaagctt gcagttgaac atgaaaaaca
     2101 gtctgttggt acatggtatc atgcatatcg tcaatgactt tgaaatacct tatataatct
     2161 ggtttaatca ttaggaacag catgatcatg agtggtcaaa gagctaccag caccatccag
     2221 agcgatataa atatctttca tcatatagaa gctatttgta taagtaaatc taacagtatt
     2281 gggctccccg caatacaaaa tcacttggga cagctgatcc aaagagaacg tctgggtctt
     2341 ttcttctcga taacctattc caaagctccc acttgaagta cttactgaat ctgttgcaac
     2401 agaaaaacaa tccctactac tagaataata gcacctctaa ctaataaatg actcattcca
     2461 acattcacat cttttaacat catgttcctt tcattatttt ttatttagga attcgtcata
     2521 cta
//
LOCUS       DATROY010000174         2506 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.175, whole genome shotgun sequence.
ACCESSION   DATROY010000174 DATROY010000000
VERSION     DATROY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.175"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            281..1621
                     /locus_tag="VBD11_001922"
     CDS             281..1621
                     /locus_tag="VBD11_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6954828.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
     gene            complement(1723..>2506)
                     /locus_tag="VBD11_001923"
     CDS             complement(1723..>2506)
                     /locus_tag="VBD11_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954829.1"
                     /translation="FKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQTLLTPA
                     PLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVFFTSNL
                     IGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVYLVKID
                     FNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGNFQHLY
                     FSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
BASE COUNT          817 a          443 c          382 g          864 t
ORIGIN      
        1 aactaatcaa acaagaatcg atagtattat tggattatat ttcttaaaaa attcactaca
       61 gatatagttt actccactta aataacttag gtaaatctac ggaactaaaa ataatcgata
      121 gaaatctgtt attttgttat ttcaaatgta actgttaaga aaatagttct ctaatactaa
      181 acagcacctc aatcgattat taagtagcat taatttttaa ttgtttttct attttctatt
      241 tgttataatg gcttgagatt actagaaagt atagtattac atgaatcaaa atcattccgt
      301 caaaagatcc ttatccgaag ttaatcagag cgttgaggtt cctcacaata gctctttttg
      361 gaacacttta agagcttttc ttggacctgg agctttggtt gcagttggtt atatggatcc
      421 aggtaactgg attactagcg ttattggtgg agctacctat cgttacttac tattgtttgt
      481 tgttttagta tcttctttga tggcgatgca attacaacaa atggctggca agcttggtat
      541 tgttacacga caggacttag cacaagcaac agcttctcga ctcccaaaac cgcttcgtta
      601 cctgctattt attatcattg agttagccct tatcgctact gatttagcag aagtcattgg
      661 ctctgccatt gctcttcact tattatttgg ctggcccctg ttactgtcta tcatgattac
      721 tattttggat gtattcttac ttttactctt aatgaagtta ggcgtccaaa aaattgaagc
      781 ctttgtttct gttctcatat tgacaatttt gataatcttt acatatttag ttgttttatc
      841 acaacctgat ttagatgcta tgttcaaagg atttttacca caccatgagc tttttaacat
      901 atcacatgaa gggaaaaata gtcctttaac cctcgctctt ggcattatcg gcgcaacagt
      961 catgccacat aatctttatt tacattcttc tttatctcag actagacgcg tggattatca
     1021 taataaatct tcaataaaaa aagctgtacg tttcatgacc ttagattcta acattcagct
     1081 atcacttgca tttgtcgtga attcgctact attagtttta ggagctagtc tattttatgg
     1141 gcatgctaat gatatcagtg ccttttctca aatgtatctt gccttatctg ataaaactat
     1201 tactggtgca gtagccagtt ctttcctttc tacccttttt gcagttgccc tactagcaag
     1261 tggtcaaaat tcaacaatta caggtacttt aacaggacaa atcgttatgg aaggtttcct
     1321 tcacttcaaa ttacctcaat ggttaataag gctttgtact agattattaa ctcttcttcc
     1381 aatttttgct atcgccctac tagttggagg cgaagaaaat acgttagatc aactaatcgt
     1441 ctattcacaa gtttttcttt ccttagcttt accattttca atctttccat tgatttattt
     1501 cacatcgcaa aaatcaatta tgggagagca tgctaatact aaatggaata cctatttagc
     1561 ataccttatc gctatcattt taacattact caatttgaaa ttaatcatgg atttacttta
     1621 atacaataca aaaaagcccc actaatgaac tgaacctcta tcatgagaca tacatctaat
     1681 gatagaggaa agtacataat gatggagctt tttgtatttc ttttaaagaa cccgtgagta
     1741 aggactagta cgtttttgct tgtacctctc cttgacatta taataaccga tgatgaaaaa
     1801 cgatacgcca aggataattg ctgcaatcaa agataaaatt tggaaactaa aatataggtg
     1861 ttgaaaattt ccttctaaat gtgctttaaa aaccatcaac caagcccaaa aaacggataa
     1921 gattaagtaa gcattttgta ataccctagc caggtaaaga cttaaaacag ttataaaaat
     1981 caaggcaatg atagctatat atacctttgg taatgggtaa ttaaaatcaa ttttaactaa
     2041 gtagaccgct aaattgacca atgacgcaac tagcaaccat cctgcatgaa ttccaaatgt
     2101 tattctaagc agtaattttg atttattttt acttagtatc ttgataatga taaccagaag
     2161 aatacagtaa gcaaaaatta ttattgttga taggccaatc aaattgctcg taaaaaatac
     2221 agtccatata ttattaacta atagaagacc caaaaagtat ggaaaaatgc tagcaaagtt
     2281 atcttggtaa gattgactct tactgaatat agctcttaat attactaaaa aggtcaataa
     2341 atagatgact gaccaaattg aaaaggctaa tggtgctggt gtcaagagcg tttgataacg
     2401 atcagacaga ctcttttgac tgttccctgt taacaaacct gttgcggata aataattcac
     2461 tattattgtt aagataaaga ctaacaataa catcttactt ttaaat
//
LOCUS       DATROY010000175         2448 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.176, whole genome shotgun sequence.
ACCESSION   DATROY010000175 DATROY010000000
VERSION     DATROY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.176"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..188
                     /locus_tag="VBD11_001924"
     CDS             <1..188
                     /locus_tag="VBD11_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6954830.1"
                     /translation="YDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVESSLP
                     SELELIRTLIAFSKDKK"
     gene            185..>2448
                     /gene="secA2"
                     /locus_tag="VBD11_001925"
     CDS             185..>2448
                     /gene="secA2"
                     /locus_tag="VBD11_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6954831.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDLTEHSLRRYILENLTYKFKYFPDEFDV
                     HNSDDVFKLLIDIFDREFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEVDSLQQLK
                     GVVTNRGIGQRDII"
BASE COUNT          882 a          327 c          484 g          755 t
ORIGIN      
        1 tttatgatat ttctcttatt ggtgcgggtt gtataagtgt agaattttgt tacctagaat
       61 tatttgaagt aattgagact caagaagaaa aagatggtat tgaactaaac agttatgttg
      121 aaagcagctt accatcagag cttgaactta ttagaacgtt gatagcattt tctaaggata
      181 aaaaatgaaa aaagttgttg ttaaacccaa aatctctatt ctaaataaat tgagattgca
      241 aagaattcaa aaaattgtaa acgagataaa taataaaaaa gtgtactacg ctcaattatc
      301 agataaggag ttaagccatc aaactttaat attaaaaaat agactttcaa gtggagaaag
      361 tctagatgat attcttgttg atgcttttgc tgtgataaga gaggctaata aaagaatttt
      421 aggtttgttt ccatatgatg ctcaggttat gggtgctatt gctcttcatc aaggaacgat
      481 agctgaaatg aaaacgggag aaggaaaaac attaactgca actatgccct tgtatctgaa
      541 tgctttaact agcaagggag caatcttagt gacgaccaat gattatttgg caaaacgaga
      601 tgcattagag atgggaaaag tatatcaatt tctaggtatg acagttggta ttaatgtttt
      661 tgataaaaaa gaagaagcag atgcgactat taagagagaa gtatatgaat ctgatattac
      721 ttatactact gcaggtgcct taggtttcga ctatctcgtt cacaatctgg ctagtaataa
      781 actagaacaa tttctaagac cttttcattt tgttatcgtt gatgaagcag actctgtttt
      841 gttagatatt gctcaaacac ccttaattat agcgggagat cctcgtgtac aatctaatct
      901 ctatggtatc actaataatt ttgtactcac tcttaaggaa aatgatgaat atatccataa
      961 agctaaggat aaaattgtct atcttactga gaagggtgta agttacgcaa aacagtactt
     1021 caatatttca gaattatata atgatgagta ttgggaatta aatagacata taaacctagc
     1081 attgcgtgct cattgtttgt ataaaagaga ttatgattat gtagttaaaa ataatgaagt
     1141 aaaattgtta gataacgcaa caggtcgtgt tatggaaggc acaaaacttc aatcagggat
     1201 tcaccaagct attgaagcaa aagaagatgt tgctttgaca aatgaatcta gggcaattgc
     1261 ttcaataacg tatcaaagtc tctttaatat gttcccaagg ttgtctggga tgacaggaac
     1321 tggaaaatct tctgaagatg aacttataaa aacttaccac ctgcctgtta ttgttatacc
     1381 aacaaatttt ccgatcaaac gagtagatct tgccgataaa atatatgtta gcttaccaga
     1441 aaagttgcaa gctactatag atgatgttaa acaaagacat agtaaaggtc agccagtatt
     1501 gttaatttct ggaacagttg aaattgctaa catttattca aagttattgc tgcgagaagg
     1561 cattgcccat aataccttaa cagctgataa tgttgttaaa gaggcacaaa ttataaaaga
     1621 agcgggacaa aaaggttcag taacatgtgc aacagttctt gctggtcgtg gaacagatat
     1681 taaactaggt gaaggggtta gagaattagg aggacttgca gttataggaa cggaaaggat
     1741 gcctaattta cgtatggact ggcagcttag gggccgttcg gggcgtcaag gagacccagg
     1801 gattagccaa ttttatgtgt cgctagagga tgaattaatt gtttctcatt ctccggaatg
     1861 ggtgataaag tatttaaaaa aatattctcg taaagaaact tcaaattact atatggtacc
     1921 tagaaagaaa cgcttttttt atcaaatagt taaaaatgct caactcagaa gtgaagataa
     1981 aggtgtatct tctagggagc aaactattaa gttcggagag agcttgcgta tacagcgtga
     2041 aaatatttat caattacgaa atgaattaat agctgatagt tccattgtgg ttgatggtgt
     2101 gataaaaatt attcaggata attttaacga tattgctaat gataaggatt taacggaaca
     2161 ttctttacga agatatatat tagaaaatct tacctataaa tttaaatatt tccctgatga
     2221 atttgacgtt cataattctg acgatgtatt taaattattg attgacatat ttgatagaga
     2281 atttactgca aagaaaacta aacttcaaag tgataatgaa tttgataatt tcgtcagaat
     2341 atcgatatta aaggctattg acaaatcgtg gatagaagaa gtggatagcc tacagcaatt
     2401 aaagggagtt gtaaccaata gaggtatcgg tcaacgagat attattca
//
LOCUS       DATROY010000176         2389 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.177, whole genome shotgun sequence.
ACCESSION   DATROY010000176 DATROY010000000
VERSION     DATROY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.177"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..274)
                     /gene="asp3"
                     /locus_tag="VBD11_001926"
     CDS             complement(<1..274)
                     /gene="asp3"
                     /locus_tag="VBD11_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6954832.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQIN"
     gene            complement(267..1856)
                     /gene="asp2"
                     /locus_tag="VBD11_001927"
     CDS             complement(267..1856)
                     /gene="asp2"
                     /locus_tag="VBD11_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6954833.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWFTSQYKTILSDDFGRVFSD"
     gene            complement(1858..>2389)
                     /gene="asp1"
                     /locus_tag="VBD11_001928"
     CDS             complement(1858..>2389)
                     /gene="asp1"
                     /locus_tag="VBD11_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6954834.1"
                     /translation="AKMEVNPSLELKIVSYKNDYDMTSLKEQVKDKILSSKNPDYFFT
                     SLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYTQIAAL
                     SSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVKKMGDY
                     TSGKILQRWRKLLEKE"
BASE COUNT          795 a          441 c          316 g          837 t
ORIGIN      
        1 aatttatctg aataatcacg catccctcag gcacagtagt taattttgat accaactcat
       61 aggtatgatt aggttttagt aaggtaagct ctggcaacgt tctattagct tgaaaattcg
      121 ttttagaaag ccattttttg attgtttttc ctgtagagaa tcttagattc tcaaaaaaaa
      181 cacgattatg ctccaaatac tttatagtcg aaccataaag ataaacattg ctactatcag
      241 tccaaagcac cttctgtatt ctttttttaa tcactaaaca ctctcccaaa atcatcactg
      301 agaattgttt tatactgaga agtaaaccaa ttatttattg attgactatt atcattatgt
      361 cgcccagcat atccacttgc atacaacagg gtgttagttt tagaaaaata atcaatcagc
      421 cttggagttg catttctatc ataatcatca tgtttcatat aagatatagc aaactgcgtt
      481 ttttcaaaat tagaacgctt aaacttattc caaaattttt gattaaattg attaatagct
      541 tcatcgtttt gagaaccaat aatattttgg agaatatcca tactcgtttc aaaatcatct
      601 ggtcttctta attttaaatt tactaccata tcgcctaaat ttgtaaaggg tttccccaca
      661 actatagcat ggggggaaaa agtagaacca taataaaggg caccaaaact tcccatagat
      721 aaacctgata aaataagttg acaatgatca aattgtaggt aatctaaata tttttgtaaa
      781 acattttcta attcactttc gtattctgaa gtacctaggt agaagcttcc accttcaatc
      841 cttggatcac caattaagag aaacggagct cccaaacgtt tcatcatgta aaaaccctca
      901 aaaccttcgg ctcctcgaaa acctgaaaaa taaacattca atggaggttt catatctcca
      961 gggttaaaat agtagataaa ctcttgcctt ttggaatcac tgtacctttc tccaccgatt
     1021 aaaaattgac ccaaaccttt acgcgaatat cgccaatgta gtgcaccaaa tctcaccact
     1081 ccgtcacctc tagcaaagat agaaattgat gaaaaaccaa ctgattcatc atattctaaa
     1141 atatatggat cagccaaatc attttctgaa aggtattttg tatttaatat caaatcaagt
     1201 gatccttttt ggtattgaat gacttctatc ataatactat tatcaccttg caatttttga
     1261 tattcttgcc atatttctat cgctttatca gtagaggata aattatagcg aaatgagaat
     1321 agaggttgaa aatcttctcc aaaattccct gaaaaacaaa tagagctatt gccttcatat
     1381 attatctctc ccttaaaatc aggatttaga tctatgtcag aaaccttcaa ttttgcacca
     1441 tattgttccg aaaaaagagt acggcttagg aaaataattt tttctttcct tgatccatta
     1501 aatggtagtg gtctcaaaac cttacgttta aatataccgt tttctgagca aaaatcgagt
     1561 tgaaaatcct cgtcgtaaaa gagtgtatgc gcttctaagc aatctgaaag taggttcaat
     1621 tgcaattcgc taacctcact agttaaaatg accgcattaa accataattt aacttcatta
     1681 gttgaagttt taccaatata ttcaacatta tttttttcta actctttact ttttaatttc
     1741 tctaagaaaa tttgaatagc atttccatta cagtataacc attcaatatt gtctggagtt
     1801 attaaatcag catcccatgg ttcctcacca atctgcaaaa tttttaattt ttccatatta
     1861 ttctttttct aataattttc gccagcgctg taatatctta ccgcttgtat aatcacccat
     1921 tttttttacg ctgtaaacta acgatttatt ccaattcatt agtccatcaa aataatagtc
     1981 aattgcatta ttgagttctg tagagtcact aataatccag ccattcttct ggtgttctac
     2041 ataattagtg tgaactttat tgatttgagg aattccagaa ctcagagcag caatttgagt
     2101 atataggtct ggatcatctc ttaaatcaat aactaaccta gcagtatcta gagcatctat
     2161 aatttgattt tcatttgtat atatatcaaa actaatgcca cgaattttag ttttttgttc
     2221 ttccttaaaa tgattctcat cttccaaaga aagagaggta aaaaaataat ctggattttt
     2281 agaagacaat attttgtcct taacttgttc ttttagagaa gtcatatcat aatcattttt
     2341 atagctgact attttcaact ctaatgacgg attaacttcc atttttgct
//
LOCUS       DATROY010000177         2371 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.178, whole genome shotgun sequence.
ACCESSION   DATROY010000177 DATROY010000000
VERSION     DATROY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.178"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..937
                     /locus_tag="VBD11_001929"
     CDS             <1..937
                     /locus_tag="VBD11_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6954835.1"
                     /translation="RNKYDHNYIAVCILDSSEKDCYDLKHNSLRIINKDALTSELTCL
                     TVDQAFINIPIELFGKYQIQDIINDIEAMGVIVNVNVEALSFDNIGEKRIQTFEGYSV
                     ITYSMKFYKYSHLIAKRFLDITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGR
                     NGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLEDDPRITKIGKFIRKTSIDEL
                     PQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDE
                     IVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            961..1410
                     /locus_tag="VBD11_001930"
     CDS             961..1410
                     /locus_tag="VBD11_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6954836.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1410..1883
                     /locus_tag="VBD11_001931"
     CDS             1410..1883
                     /locus_tag="VBD11_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6954837.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
BASE COUNT          803 a          308 c          456 g          804 t
ORIGIN      
        1 taggaataaa tacgaccata attatatcgc tgtctgtatc ttggactcct ctgaaaagga
       61 ttgttatgat ttgaaacata actcgttaag gataataaac aaagatgctc ttacttcaga
      121 gttaacctgc ttaactgttg atcaagcttt tattaacata cccattgaat tatttggtaa
      181 ataccaaata caagatatta ttaatgacat tgaagcaatg ggagtgattg tcaatgttaa
      241 tgtagaggca cttagctttg ataatatagg agaaaagcga atccaaactt ttgaaggata
      301 tagtgttatt acatattcta tgaaattcta taaatatagt caccttatag caaaacgatt
      361 tttggatatc acgggtgcta ttataggttt gctcatatgt ggcattgtgg caatttttct
      421 agttccgcaa atcagaaaag atggtggacc ggctatcttt tctcaaaata gagtaggtcg
      481 taatggtagg atttttagat tctataaatt cagatcaatg cgagtagatg cagaacaaat
      541 taagaaagat ttattagttc acaatcaaat gacagggcta atgtttaagt tagaagatga
      601 tcctagaatt actaaaatag gaaaatttat tcgaaaaaca agcatagatg agttgcctca
      661 attctataat gttttaaaag gtgatatgag tttagtagga acacgccctc ccacagttga
      721 tgaatatgaa aagtataatt caacgcagaa gcgacgcctt agttttaagc caggaatcac
      781 tggtttgtgg caaatatctg gtagaaataa tattactgat tttgatgaaa tcgtaaagtt
      841 agatgttcaa tatatcaatg aatggtctat ttggtcagat attaagatta ttctcctaac
      901 actaaaggta gttttactcg ggacaggagc taagtaaagg taaggtttga aaggaatata
      961 atgaaaattt gtctggttgg ttcaagtggt ggtcatctag cacacttgaa ccttttgaaa
     1021 cccatttggg aaaaagaaga taggttttgg gtaacctttg ataaagaaga tgctaggagt
     1081 attctaagag aagagattgt atatcattgc ttctttccaa caaaccgtaa tgtcaaaaac
     1141 ttggtaaaaa atactattct agcttttaag gtccttagaa aagaaagacc agatgttatc
     1201 atatcatctg gtgccgctgt agcagtacca ttcttttata ttggtaagtt atttggttgt
     1261 aagaccgttt atatagaggt tttcgacagg atagataaac caactttgac aggaaaatta
     1321 gtgtatcctg taacagataa atttattgtt cagtgggaag aaatgaaaaa agtttatcct
     1381 aaggcaatta atttaggagg aattttttaa tgatttttgt cacagtgggg acacatgaac
     1441 agcagttcaa ccgtcttatt aaagaagttg atagattaaa agggacaggt gctattgatc
     1501 aagaagtgtt cattcaaacg ggttactcag acttcgaacc tcagaattgt cagtggtcaa
     1561 aatttctctc atatgatgat atgaactctt acatgaaaga agctgagatt gttatcacac
     1621 atggcggccc agcgacgttt atgtcagtta tttctttagg gaaattacca gttgttgttc
     1681 ctaggagaaa gcagtttggt gaacatatca atgatcatca aatacaattt ttaaaaaaaa
     1741 ttgcccacct gtatcccttg gcttggattg aagatgtaga tggacttgcg gaagcgttga
     1801 aaaggaatat agctacagaa aaatatcagg gaaataatga tatgttttgt cataaattag
     1861 aaaaaattat aggtgaaata tgaggaaata tctagattta gattattctt tattttatgc
     1921 tctttgggta cttattttag taccaaacca atggtatcag tttttaatta ttaccattat
     1981 agttctatta ttactttgga agagtgagtt tagaatatct ataagcaatt cttcaatact
     2041 atttctgctt tggttattta tttatttatt tgcaatactc attagaggta ctcaagagga
     2101 tataacgttt cagcgattta ttgctgagct attaaaacta attagtacag gatatgcttt
     2161 atttttttat aattattata gaaaagctga ttttaatagt tcagttgtaa ggaatgtggt
     2221 aaaggttaac tattttgtgt tgtttcttat aacagtttta tatttatttt ttcctatgct
     2281 gaagccaact ttatttggaa gagaattgtt ttcaatagag tggtttccac atatgagaat
     2341 aagacttgcg gcatattttg aatatgctac a
//
LOCUS       DATROY010000178         2324 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.179, whole genome shotgun sequence.
ACCESSION   DATROY010000178 DATROY010000000
VERSION     DATROY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.179"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(469..1917)
                     /locus_tag="VBD11_001932"
     CDS             complement(469..1917)
                     /locus_tag="VBD11_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEO6954838.1"
                     /translation="MKNNDLSHYVAHYQFSPESQYLDRKSARKKPSELLKHLIAFANA
                     DGGQLVIGIEDEKKGNLITGFKDGKAYPIDEFKKIDREMRETPLNLSFEEIPVVNYKG
                     EDDLILIISVELSSNRVIAAPNDDVYLRQGDESIKLSYEQRTQLSYDKGQRFFEDETV
                     LDATLEDIDENIVQDFKSHFDLSERSTEEILKARRFLVNGKLTKAAILLFGKYPSAFF
                     PQARVRFQRFDGTDMGTGSNFNVIKEVTFDDALPVLIIKVRDFIRTQLREFQYLDDNG
                     QFQILPEYPEFAWFEGIVNAVTHRDYSVYGDHIRVLMFDDRLEIHSPGKLPNIVTVEN
                     IKHERFSRNPRIARTLTEFGWVREMNEGVKRIYSEMESAFLHEPKYSEPGNKVVLTLE
                     NSITSRTLRTRDSLEIKVEDYSKLSVDEKLLLQHMYNTGEQMTTKKASELLGRSVSYS
                     SKLLKRLRDKKILTWYGTSTRDRSQYYKLEMK"
     gene            complement(1973..>2324)
                     /locus_tag="VBD11_001933"
     CDS             complement(1973..>2324)
                     /locus_tag="VBD11_001933"
                     /inference="COORDINATES: protein motif:HMM:NF028183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954839.1"
                     /translation="FSFDKEKLYSSSYPELEVGTEIGVVRPREIISLELFGAEIQFLE
                     YTIYPRMTFKRMLDENKEEIKMIFDLPEDSNYYVLFSLDSIDDFDMSKSGRDLFDLTD
                     SAITLQDIDFSVLD"
BASE COUNT          747 a          460 c          345 g          772 t
ORIGIN      
        1 tatattcgcg aaggggttat caggttatca aaagtaaaat tgacaataaa aaaacacctt
       61 gcaattgctt gcaaagtgct ttgaaacgtt gatataacag tatattaacg ttttgaaaat
      121 tgtgatgctt tacgtgcttt tttaagacct ggttttgggt aaaaaaaatc aaatatcttt
      181 aaaagcctat taaactaagc tttcaaactt cattttcgtt gaaaataagc acttataacc
      241 taatcttaca aacaatggat taccattaga ttaccaaaaa attcgttttc ggagcttatg
      301 taattgagaa atcgcattta tttcatttgg tatattatgc ctaggttcag aaactaaaat
      361 taatcgataa tatccgaaaa gtttttcaaa ctattgtaag ataatactcc aatttgaaaa
      421 taaactcaaa aaaccttttt cttcgttttt cttcgttttt cttcgttttt acttcatttc
      481 taacttgtaa tactgactac gatcccttgt actagtgcca taccaagtta aaattttctt
      541 atcacgtaat ctctttaaca attttgatga atatgacaca ctccgcccca ataattctga
      601 ggcttttttt gtggtcattt gttcaccagt attatacata tgttgaagta ataatttctc
      661 atctacactt aatttgctat aatcttcaac cttaatctct aaggaatccc ttgtacgtaa
      721 tgttcgactc gtaatgctat tctctaacgt caaaactacc ttattacccg gttctgaata
      781 tttaggctca tgaagaaaag ccgactccat ctcagaatag atacgcttaa ccccttcatt
      841 catctcacga acccaaccaa attcagtcag ggttctcgca atccgaggat ttctagaaaa
      901 acgttcatgc ttgatgttct caaccgttac aatattcggt aacttacctg gactgtgaat
      961 ctctaagcga tcatcgaaca ttagaacacg gatatgatca ccatagacag aataatcacg
     1021 gtgagttacg gcattgacaa taccttcaaa ccaagcaaat tctgggtatt ctggtaagat
     1081 ttgaaactga ccattgtcat cgagatactg aaattctcta agctgcgtac ggataaagtc
     1141 cctaacctta ataatcagca ctggcaaagc atcatcaaag gtcacttctt tgatgacatt
     1201 aaagttactc cctgttccca tatctgtccc atcaaatcgt tggaaacgaa cacgtgcttg
     1261 agggaagaaa gctgatggat acttcccaaa aagtaaaatc gcagctttag tcaatttgcc
     1321 attaaccaaa aatcgtctag ctttgaggat ttcctctgtg gagcgctctg ataaatcaaa
     1381 gtgacttttg aaatcctgta cgatattttc atcaatatct tccagtgttg catctaaaac
     1441 agtctcatct tcaaaaaagc gttgcccttt gtcatagcta agttgtgttc gttgttcata
     1501 acttaacttt atcgactcat caccttgacg aaggtaaacg tcatcattcg gtgcagcaat
     1561 aacgcgatta gaagacaatt caactgagat aatcagaatg agatcatctt ctcccttgta
     1621 attaaccaca ggaatttcct caaaggataa gtttaatggt gtctcacgca tctcacggtc
     1681 aattttttta aactcatcaa taggataagc tttgccatcc ttgaaaccag tgatgagatt
     1741 tcctttcttc tcatcctcaa taccaataac taactgacca ccatcagcat tggcaaaagc
     1801 aattaaatgc ttgagcagtt ctgacggttt tttacgtgca gattttctat ctagatattg
     1861 actttctggt gagaattggt agtgggctac atagtgagat aaatcattat tttttatcaa
     1921 aattaatcct cctctcgact gcaacaatag aacttcctag ttgcagaata ttttaatcta
     1981 gtacagaaaa atctatgtct tgtagagtta ttgcactatc tgttaagtca aataaatccc
     2041 gtccagactt agacatatca aaatcatcta ttgaatccaa tgaaaatagt acataatagt
     2101 tactatcctc aggtaagtca aagatcattt tgatttcttc tttgttttca tctaacattc
     2161 ttttgaaggt catccttgga tatatagtat attctagaaa ctgtatctca gctccaaata
     2221 attctaaaga gattatctct ctaggacgta ctacacctat ttcagtaccg acttctaatt
     2281 caggataact tgaagaatat agcttctctt tatcaaatga aaaa
//
LOCUS       DATROY010000179         2315 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.180, whole genome shotgun sequence.
ACCESSION   DATROY010000179 DATROY010000000
VERSION     DATROY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1385
                     /locus_tag="VBD11_001934"
     CDS             <1..1385
                     /locus_tag="VBD11_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO6954840.1"
                     /translation="IQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNLDIQWGNHDV
                     TWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYEPLPSFAPIL
                     DGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQVNYKDLSIS
                     IKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHIDFLFEKGSM
                     YLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAYARPEKHDDL
                     ATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDKDDICTALLQ
                     EFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGLAGYTLIYNS
                     YGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKLTKEIKDLDH
                     LYRHFQEYDD"
     gene            1475..>2315
                     /gene="queG"
                     /locus_tag="VBD11_001935"
     CDS             1475..>2315
                     /gene="queG"
                     /locus_tag="VBD11_001935"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6954841.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFK"
BASE COUNT          764 a          354 c          456 g          741 t
ORIGIN      
        1 caattcaagt tttaatcatt gaccatttac atgttgtagg ggatatttac gatcgtggta
       61 ggtatcctga tcgtatcttg aataggttaa tggcttttcc aaatttggat atccaatggg
      121 ggaaccatga tgtcacttgg atgggagcag catctggttc ctacctttgt atggtaaatg
      181 ttatccgtat tgctgcacgt tataataata ttactttaat tgaagaccga tatggtatca
      241 atttgcgtcg cttggttgat tactcacgcc gttactatga acctctacct tctttcgccc
      301 ccatattaga tggagaggaa atgactcatc cagatgaatt agatttgtta aatatgatac
      361 aacaagcaac agcaatttta cagtttaaat tggaagccca attgattgac aggcgtccag
      421 aatttcaaat gcataatcga caacttatta accaggtaaa ttataaagat ttaagtatat
      481 ctataaaggg agttgtgcat caacttaaag attttaatag tagatgcatt gattcgaaaa
      541 atcccagtcg attaacaagt gaggaagaag agcttttaca acaattgatg atagccttcc
      601 agacttctga aagtttaaag aaacatattg atttcctatt tgagaaagga agtatgtatt
      661 taacctataa tgataatctt ttattccatg gttgcatacc aatgcatagt aatggtgatt
      721 ttaaaagctt taaaattgca ggaaaaactt atggtggtag ggatttactg gatttatttg
      781 aaagtcaaat acgtctagca tatgctagac cagagaaaca tgatgatttg gcgacagata
      841 tcgtttggta tctttggtgt ggtgaaaatt cttctctttt tggtaagaat gccatgacta
      901 cttttgagcg ctattatgtt tccgacaaag taactcatca agaaagaaaa aatccctact
      961 ttaaactgag agataaagat gatatttgca ctgccctttt acaagagttt gatttgccta
     1021 agtttggaca tattgtcaat ggtcatacgc cagtcaaaga aaaaaatggt gagcaaccga
     1081 taaaagcaaa tggtaagatg ttagtaattg atggtggctt tgccaaaggt tatcagaaaa
     1141 atacaggttt agctggctac actttgattt ataatagcta tgggatacaa ttaatttcac
     1201 accttccttt tacatccatt gaggaagttt taagtggaac caactatatc attgatacga
     1261 agcgattagt agaagaagcc aaggatagga ttcttgtaaa agatacaact attggacaaa
     1321 agttaaccaa agagattaaa gacttagatc acctttatcg tcattttcag gaatatgatg
     1381 actagtgttt aagtaattta ctaattgata tgtaggattt aatttttcag tttcttacct
     1441 tgtctctttt attaaattga cttaaaataa tattatggac ataaaggcag aaattcaaaa
     1501 attagcaaaa gaaattggta tctctaaaat tggttttacg acggcagata actttgatta
     1561 tttggaaaag tcattgcgtg cttctgtgga agaggggcgc aattcaggat ttgaacataa
     1621 agtcatagag gatcgtatct accctgaaag gcttttagaa tcggctaaaa cgattatttc
     1681 aattggcgtt gcttatcctc acaaacttcc tcagcaacct caaaaaacac cttataaacg
     1741 aggaaaaatt acacctaatt cgtgggggct tgattatcat tatgtggtcg gcgaaaagtt
     1801 agacagacta tcaaaaggca ttgaagagct ctgtcgagat tttcctttgc aacaaaaagc
     1861 aatggtcgat actggagctt tagttgatac tgcagttgcc caaagagcag gtattggttt
     1921 tataggaaaa aatggtctag ttatttctaa agaatatggc tcttatatgt ttttgggaga
     1981 attaattacc aatttagaga tcgagccaga caagcctgtc gattatgatt gtggtgactg
     2041 tcgtcgttgt ctagatgcct gtccaacttc ttgtttaatt ggtgatggct caatgaatgc
     2101 caagcgttgt ctatcatttc aaacccaaga taagggaatg atggacatag agtttcgaaa
     2161 aaagattaaa acagttatct atggttgcga tatttgccaa atttgctgtc cttataacaa
     2221 aggtataaat aatccattgg cgacagagat tgatcctgag ttggctcaac cagaattaat
     2281 accatttttg agtttatcaa atggacaatt taaag
//
LOCUS       DATROY010000180         2314 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.181, whole genome shotgun sequence.
ACCESSION   DATROY010000180 DATROY010000000
VERSION     DATROY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            122..1477
                     /gene="rlmD"
                     /locus_tag="VBD11_001936"
     CDS             122..1477
                     /gene="rlmD"
                     /locus_tag="VBD11_001936"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6954842.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
     gene            1745..1996
                     /locus_tag="VBD11_001937"
     CDS             1745..1996
                     /locus_tag="VBD11_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954843.1"
                     /translation="MIINSFDNSRKATINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            1993..2298
                     /locus_tag="VBD11_001938"
     CDS             1993..2298
                     /locus_tag="VBD11_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954844.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIPHLDMVLS"
BASE COUNT          799 a          302 c          458 g          755 t
ORIGIN      
        1 ttttcgattt tggttatttt catagttttt attatagcaa aaatgataaa atagtaaaaa
       61 tgagtctttt tttaaatgtt atggatttat agtattttct tagttaggga gtaatgaact
      121 gatgaatgtg gttttaaaac agcgaattcc tttaaaaatt aaacgaatgg gaattaatgg
      181 tgaaggaata ggtttttata aaaaaacact aatctttgtt ccaggggctc tcaagggaga
      241 agaggtgttt tgtcagattt cttctgttag acgtaatttt gcagaagcta aactgttaaa
      301 gataaataaa aagtctaaaa atagggtaga tcccgcttgt tctatttata aagaatgtgg
      361 gggatgccaa ataatgcacc ttcaatatga taagcagcta gagtttaaaa cggatattat
      421 tcgacaggct ctaatgaaat ttaaaccaga gggatatgag aattatgaaa tccgtaaaac
      481 gataggtatg tcggaacctg aacactatcg tgctaaatta caatttcaag tgcgttcgtt
      541 tggagggaat gtaaaggcag gtttatatgc acaagggaca catcgattga ttgatatcaa
      601 agattgttta gtgcaagata ggcttactca ggaaatgata aatcgagttg cagaactttt
      661 aggaaaatat aaattgccta tttataatga acgtaaaata gctggtgttc gtactgttat
      721 gattcgacga gcacaagcga gtggagaggt tcaactgatt tttatcacaa gtaaaagact
      781 tgattttgat gatgttgtta ttgaattagt tcgagagttt cctgagttaa agacagttgc
      841 tgtaaatatc aacgctagta aaacaagtga tatctatggt caaataacag aagttatttg
      901 gggtcaagaa tccatcaatg aagaagtatt agattatggt ttttcgttat caccacgtgc
      961 tttttatcaa cttaatccta aacaaacaca aattttatat agtgaggcag tcaaggcctt
     1021 agatgtgaag gaagatgatg atttaattga cgcttattgt ggagtaggaa cgattggact
     1081 tgcttttgct ggaaaagtta aaagtgttcg tggaatggat attatccctg aggctattca
     1141 ggatgctaaa gagaacgcac ttcatatggg ttttactaat acacattacg aagctggaaa
     1201 ggcagaggat attatcccta ggtggtatag tgagggattt cgcgctaatg cgttaatcgt
     1261 ggatcctcct cgcacaggct tagatgataa attgctaaat actatactaa aaatgcctcc
     1321 agaaaaaatg gtttacgttt cttgtaatac atctacgctt gctagagact tagtaacatt
     1381 aacaaaagta taccatgttc actacatcca atctgttgac atgtttccac atacagcaag
     1441 gaccgaggct gtagtgaaac tacaacgtaa ggtataataa agttttctaa tctaaaaata
     1501 agagtatttt tgggtattct tatttttaga tagtaatggt gagatattaa tatagcatag
     1561 tataaaacac atggtttaag ctatttgata cggaaattta ccttagaaat ggtaaataat
     1621 tccattaagt atattcatct ttgcaaattt gaccttgcct tttaagtttc attgggttaa
     1681 aatagaaaaa atatttttaa caagatgata gaacatcgta aattaaggtt taaaggaaat
     1741 gcttatgata ataaattcat tcgataatag tagaaaagcg actattaatc ctgaagatat
     1801 taattcacca ataaaaggtt ttccaaagac tgttattacc tgttttgcgc gggagacttt
     1861 caatcgcatt ttagaagaat tgcctcatcg tgaaattgct agaactagtg tagcaaactt
     1921 agaaattccc atttatgaat tagaatttaa aggacaaaag attgtcctta tcaagaggtt
     1981 ggtgatgaag atttgatttg tttgcatggg atgaattggg tagatggtca attggcagct
     2041 tattatagat tgataccaga agatgataag gtgcatttgg gacgtgtgat tgttaatcca
     2101 gactttagaa aaaagggatt aggaaatcaa ttagtggaat atgagattaa atttagcgag
     2161 gcaaattacc ctaataaacc tatttatgct caggcccaag cttatttaca agatttttat
     2221 caatctttcg gatttcaacc tatctcagat atctatctag aagataatat tcctcatctt
     2281 gatatggttt tatcataaat acatactatt taaa
//
LOCUS       DATROY010000181         2307 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.182, whole genome shotgun sequence.
ACCESSION   DATROY010000181 DATROY010000000
VERSION     DATROY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.182"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..772)
                     /locus_tag="VBD11_001939"
     CDS             complement(<1..772)
                     /locus_tag="VBD11_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6954845.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNY"
     gene            981..1454
                     /locus_tag="VBD11_001940"
     CDS             981..1454
                     /locus_tag="VBD11_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6954846.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            1456..1761
                     /locus_tag="VBD11_001941"
     CDS             1456..1761
                     /locus_tag="VBD11_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6954847.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGSILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            1801..2288
                     /locus_tag="VBD11_001942"
                     /pseudo
     CDS             1801..2288
                     /locus_tag="VBD11_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
BASE COUNT          785 a          344 c          380 g          798 t
ORIGIN      
        1 tgtaattgtt attagttaaa attttggtga agctactgta ttgctcctta acaacattat
       61 caatattatg atctgttaat acaaaatcaa tatcgtctaa ttgataaaaa gtataaaaat
      121 catatttacc aaatttttgg ctatcaacta aaaggaattt ttcaaaggaa ttattaagag
      181 caatgcgctg gatttcacct tctccctcgt cataagtagc gatagacttt tcaaaaacac
      241 cattactact aacaaaagct tttgaaaaat taagtgattt aatagagtta atagtaacag
      301 aaccgacaaa agcaccagta atttctctgt actctcctcc tacaaggata aggtcaagtg
      361 ttgaactttg attcagaata ttgaaaacag gaaggctatt ggttactacc ctaattttaa
      421 aatcaagtaa ttgcgtagct aatttctcta aggttgttcc aggcccaata aagattgttt
      481 caccatcatt gatgaattgt tttgcaaatt gggcaatttc taatttttca tttgtttgaa
      541 tatcgtattt ttcagtgttt gatttttcat aattttgtgg aattggtgct gcgtttactt
      601 tttgtgcacc accatggatg cgtattaaca gtcctgcttt atctaattca tccaagtcac
      661 gcctaacagt catgtctgat acatcaagtt cttccatgat ttcattaaca gtgacaatac
      721 catttatatt gactttttct attatttttt gtaatctttc tcgctttaac ataataatct
      781 ctcctacctc ttcattgtat atatcatacc ataaatagag ctaaaataat aataattttg
      841 aaaaaacaaa caataataaa caaaaataaa taaaaaacgt cttgtgatgt tttattttgt
      901 tttgtataat attgatgtag ataagttgat aaggcttgtt tgccttacaa caaaatgtaa
      961 ccgcttaact aaaaagaaag gtgtttgctg tgactcaaga tattttattt attgatgccc
     1021 attctcagga agaattattt gacttagtat caaaagcatt gataaaacaa cactatgtta
     1081 gtcctgacta ccgacaggca gtaaaagaaa gggagagaga gtttccaacg gggttaaaaa
     1141 ttgatttaaa agatgggact cctattcaat atgttgctat tccacacact gaaacgcagt
     1201 attgtctagt agatagaatc ttttatgtca aaaatagtca gccaattact ttcaaacata
     1261 tgattaatcc agaagaggag tgtagggttc aggatttttt cttcatcatt aatagtagga
     1321 atagtaacca aagtgatatt ttatctaatt tgattacctt ctttattaca aaaggaaacc
     1381 tagatcggct acatgagctt ggcgacaata aggaaaaaat taatcattat ttaatagaaa
     1441 aaggagtgtt ttaacatgat caaaatatta gcagcttgtg gtgcaggcat aaattcaagt
     1501 catcaaatta aagatgctat cgagacacaa ttaggagata gaggttacaa tgtacattgt
     1561 gatgctgtta tggttaagga tatcactgag gaaatggtca acaaatatga tatctttact
     1621 cctattgcta aaactgattt aggttttaat gtaccaattc cagttgtaga agcaggatct
     1681 attctttatc gcataccagt tatgagcgaa ccggtcttta ctgctttaga acaagttatc
     1741 aaagaacaca acctatcata acatatttaa aattggaatt agtcttaaag gagaaggaac
     1801 atgcaggtat ttttaaatat tgtcaataaa ttctttgatc cagttattca tatgggttcg
     1861 ggagttgtga tgctaattgt catgacaggt ttagccatga tatttggagt gaagttttct
     1921 aaagcacttg aaggtggtat taagttagct attgctctta cgggtattgg tgctattatt
     1981 ggtattttaa ctggtgcttt ttccgaatca cttcaagctt ttgttaaaaa tacaggaatc
     2041 aatctaagca ttattgacgt tggttgggct ccattagcaa ctattacatg gggatcacca
     2101 tatacgcttt acttcttatt aatcatgctt attgtcaata ttgttatgat tgttatgaaa
     2161 aaaaaacgga taccttagat gttgatattt ttgatatttg gcatttatct atcacaggtc
     2221 ttttaattat gtggtatgct aaacaaatga aacgccgtaa tgcagaatat gcagctaatc
     2281 aaaaataaat agaataaaca aaaacct
//
LOCUS       DATROY010000182         2216 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.183, whole genome shotgun sequence.
ACCESSION   DATROY010000182 DATROY010000000
VERSION     DATROY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.183"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..>2216
                     /locus_tag="VBD11_001943"
     CDS             <1..>2216
                     /locus_tag="VBD11_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6954848.1"
                     /translation="IIFENASNFFKILLLEIEDTDSDFDDVEVIITGTMADVIEGEEY
                     TFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKTAQRIVELYGDN
                     TIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYGLSNRAAIQIFD
                     HYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFRAAIIHTLVESS
                     MEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKVQHIGTKIFSNT
                     LFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNIQEKAIREALLS
                     KVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGRAARRMNELTGL
                     PSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFDALDSHTQVIIV
                     GDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQMRQGFLPEDFT
                     AKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQAGINNLNLIMQ
                     NLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDLIPAKYTESKQD
                     EIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQSGRMLQRNLIY
                     TAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANSQKIEDSSID"
BASE COUNT          786 a          378 c          392 g          660 t
ORIGIN      
        1 gcattatatt tgaaaatgcc agcaattttt ttaaaatatt acttctggaa atcgaagata
       61 cggatagcga ttttgatgat gtagaagtaa ttattactgg tactatggcc gacgttatag
      121 agggagaaga atatactttt tggggaacac taacccaaca tccaaaatac ggcgagcaac
      181 tacaaagtgt acgctatgaa agagccaagc ctacttcagg tggccttgtg aaatactttt
      241 ctagcgaaca atttaaagga attggtaaaa agactgccca acgcatcgta gaactctatg
      301 gagataatac cattgataaa atccttgaat cccctgaaca gttaagtaca atctcaggac
      361 tatcaaaaat taatagagaa gcattcatcg ctaaactgaa actcaattat ggaacagagc
      421 aagttttagc taaactcgct gaatatgggt taagcaatcg tgctgctatt caaatatttg
      481 atcactataa agaagaatca cttgaagtga taaacgaaaa tccttatcaa cttgttgagg
      541 atatccaagg tatcggtttt aaaatagctg accaacttgc tgaacaagtc ggtattgaaa
      601 gtgattctcc taaacgcttt cgtgctgcta ttattcatac tttagttgag tcttctatgg
      661 agcaaggaga tacctatatt gaggctcgta ctctcttgga aaaaaccatt actctccttg
      721 aagaagctcg tcaaatcgaa ctagatcctt caattgttgc taaggaattg acaaatttaa
      781 ttgcagaaga taaagttcaa catattggaa ctaaaatttt tagtaatact ctattttttg
      841 ccgaagaagg catcaaaaaa aacttacaac gaatactaaa tcagcctctt gataaacaac
      901 taaatcacaa agatattgac cgtgaaatca gagatattca aaaatcttta aatatccact
      961 acgataatat ccaagaaaaa gctattagag aagctttact aagtaaggta tttatcttga
     1021 ctggtggccc aggaacaggt aaaacaacag ttataaatgg tattattgaa gcctattccg
     1081 agttacatca tattgattta aacaaaaatg atattcctat tgtacttgct gctcctacag
     1141 gtcgtgctgc tagacgcatg aatgaactta ccggtttacc gagtgctact attcataggc
     1201 atttgggatt aaacggagat agtgactatc aaagtctaga tgactatcta gattgttcct
     1261 taattatcat tgatgaattt tcaatggttg acacttggct tgctaatcaa ttatttgacg
     1321 ctttggactc gcacacacaa gttattattg tcggagatag tgatcaatta ccgtctgtag
     1381 gtccaggaca agttctagca gatcttttaa atataaatgc acttccacac gttaaacttg
     1441 aaaagatttt tagacaatca gaagaatcaa ctattgtgac tttagctaat caaatgagac
     1501 agggcttttt gccagaggac tttacagcta aaaaagccga tcgctcatac tttgaagcat
     1561 ctgctaatat catcccaaat atgatttcta aaattgttca atctgcactt aagagcggta
     1621 ttgaagctca tgaaattcaa attttagctc ctatgtatcg cggccaagca ggtattaata
     1681 atcttaacct tatcatgcaa aatctcctca atccattaaa agataataac caatttactt
     1741 ttaatgatat aaatttccga ataggagata aagtgttaca tttagtcaac gatacagagc
     1801 ttaatgtttt taatggggat attggttaca taacggacct tataccagcc aaatatactg
     1861 agagcaagca agatgagatt tatatgactt ttgacggtca agaagttatc tatcaacgca
     1921 aagaatggct taaaataaca ctagcctatg ctatgagtat tcataaatca cagggttctg
     1981 aatttcaagt tgttattctg ccaataacga gacagagtgg tcgaatgtta cagcgaaacc
     2041 ttatttatac agctattacg agatctaaaa gtaaactaat acttttagga gaaattggtg
     2101 catttgattt cgctgttaaa aacgaggggg caaagcgcaa tacttatctg atagaaagat
     2161 ttgaaaacaa gcaagaaata gctaattctc aaaaaattga agatagctca atagat
//
LOCUS       DATROY010000183         2166 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.184, whole genome shotgun sequence.
ACCESSION   DATROY010000183 DATROY010000000
VERSION     DATROY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.184"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            4..1908
                     /locus_tag="VBD11_001944"
     CDS             4..1908
                     /locus_tag="VBD11_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6954849.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            1988..>2166
                     /locus_tag="VBD11_001945"
     CDS             1988..>2166
                     /locus_tag="VBD11_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6954850.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNS"
BASE COUNT          752 a          299 c          405 g          710 t
ORIGIN      
        1 aaaatgaaag atcgcttata taataaattc aaggattttg atagggaatt ttgtcaaaag
       61 tatattaaaa cataccaatc taatgcctat caagaaatga aagcctctgt taatttgatg
      121 atgaggaaca cttttgtatt taatgataat tgggatatgg aaccttgttc taaagcatat
      181 tgtcttgatc ctttggagtg ggataatcca gtaacagatg atccagaatg gttgtatatg
      241 ctaaatcgtc aaacttatct ttttaaattc ctggtggtct acattgttga aggcgataag
      301 tcttatctta gacaaatgaa atattttatg taccattgga ttgattgcca atttacacta
      361 aagccagaag gggcggtaag tcgtactatt gatactggga tacgttgcat gtcatggctc
      421 aaagttttga tttttttaga ttattttgga ttaataacag aaactaaaaa aattaaacta
      481 ttaactagtt tacgggagca gataacttat atgagggact attatcgtga aaaagatagt
      541 ctaagtaact ggggaatttt acaaacaaca gcaatattgg cgtgtttata ctattatgaa
      601 gatgaattaa acctacctga aattcagagt tttgctgaag aagaattatt acttcaaatc
      661 aagcttcaaa ttttagatga cgggagccag tatgaacaat caattatgta tcatgtagaa
      721 gtcttgaagt ccttaatgga actagttatc cttgcgccta aatattatct accattagaa
      781 gaaactattg aaaaaatggt tacctatcta attgctatga ctggtccgga ttattgtcaa
      841 ctagctatag gggacagtga tgtgaccgat actcgtgata ttctaacttt ggcaacactt
      901 gtgttgaaat cttctaaaac aaaatcattt tcttttgata atgttaattt agaaacttta
      961 cttttgtttg ggaagccatc aatttatctt tttgaagaaa taccgcgtgc aacaataggt
     1021 gagtctgctt atctttttcc agattctggt catgtgtgtt tacgtgatga taggcgttat
     1081 atatttttta agaacggtcc atttggtagc gctcatactc atagtgataa taatagtgtt
     1141 tgtctctatg ataaaaagaa acctattttc attgatgcag gaagatatac ttacaaagaa
     1201 gaacaactaa ggtatgattt taaacgttcg actagtcatt caacatgtac ccttgatggg
     1261 caatctttag aaatgatcaa ggactcttgg acatacaatt cttatccaaa atgtgactat
     1321 tgtcagctga cttcaaagga taggtaccat ttagtcgaag gacaactaca tgtccaaaga
     1381 gcttctgata tctattacca taagcgatgg ttgttaactt taccgcaggc cattacctta
     1441 gttattgata aggtgaattg tccaggagag catgttttaa caaatcaata tattttagat
     1501 gatcaggtca tttatgaaaa tgggcttgtt aacgacttga aattagtaag tcctacgacc
     1561 tttaatctag aagattgcct tatttctaag cggtataatc aattgacaga aagtcataaa
     1621 ttagttaaga aaataaaatt tgttgatgag gtgatggatt ataccttgat agttgatcgg
     1681 aactgtcagg tgaaatgtgt tcctttagtg caaacgaaca gtcataagga actaagtaat
     1741 agcattgcat ttgatattag gtctcaagac tttcattatt taattggagt gcatatggat
     1801 gatattatct ttggggataa actctatttg atgcaaggga taaaatgcaa aggaaaagtc
     1861 attgtatatg ataaaaataa tggcaaaatg agtcgtttaa aaaattaaga gggctattat
     1921 tatgctatac tagtactata ttaaccataa aaaaataaaa tcagtaaaat tagaaaggag
     1981 gtttgttatg tctaaaaaaa tgaccattaa tgacatcgct caattatcaa aaacttccaa
     2041 aacaacggtt tcttttttct tgaatcaaaa atttgaaaaa atgtcggatg agacgaggca
     2101 gcgtattcaa gaggtgattg atgaaacagg ttatagacca agtacgatag caagaagtct
     2161 aaattc
//
LOCUS       DATROY010000184         2066 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.185, whole genome shotgun sequence.
ACCESSION   DATROY010000184 DATROY010000000
VERSION     DATROY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.185"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            743..1596
                     /locus_tag="VBD11_001946"
                     /pseudo
     CDS             743..1596
                     /locus_tag="VBD11_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
     gene            1669..>2066
                     /locus_tag="VBD11_001947"
     CDS             1669..>2066
                     /locus_tag="VBD11_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6954851.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIIT"
BASE COUNT          720 a          281 c          364 g          701 t
ORIGIN      
        1 acatgaagga taacaaagtt tattatttac attttggtaa agcaatagat aaaaatgata
       61 agtatctcaa tcaaattata aataattaat ttctttattt tataagcccc gaaaacaaat
      121 tttttgataa ccttttgata acccctccgc gaacatattg taatacatag tgcacttttg
      181 ggatataaat tgttgatttt tagctagttt ataccatata acaccatata ttacagttgt
      241 cagagaaaac caggtttaaa aaaagcccgt aaagcatcac aatcctcaaa acgttaatat
      301 atacctatat atcagcgatt ggaagcactt catggttcac accatggggt gttttttgtg
      361 taatattcaa aaattgtcct aaagtcattt ttaaaacttc taaatacttg agaaaccatt
      421 gtaaatttaa ataactactt taaatagcac aaaggcaatg acaaagttag atgagatgat
      481 tctattttaa tttataattg aaaaaaagag tatatgatgg aggaattttg tatggtaagt
      541 actcaatata tgaaagtaac cttacgagaa aaattcccag ataaattaac atatgttgat
      601 ttagaagaac ttgtgatatg atattacaaa taaaagaaaa tgactatata tcagctcata
      661 tattggttaa agttggtaaa tataaattat tgaaattacc tcataagttt accaatgaga
      721 ttacggttag aaaggaatac taatgtttat atatggccct atttttaggg aatttcgact
      781 taatcgacag ttttctctta aacaggttgc ttcaaatgaa ctttctgttt ctcagttatc
      841 acgatttgag aggggtgaat cagatttatc attaacaaaa tttttagggg ctttagaggc
      901 aattgattta tcaattagcg aatttatgga tagagtaaat aaatatcaaa aatctgatca
      961 gtaatgtctc agatggctca atatcattat caaagagacg tagctgggtt agaaaagatg
     1021 atttctgtgg aagaaggtaa actaaaaaag gatagttcag atatacgttg tcgtttaaac
     1081 atagttcttt ttagaggaat gatatgtgaa tatgactcct caagaaaaat gtcggaagaa
     1141 gacctttgct ttttgtcgga ttacctcttt caaaaggata gttgggaaat tagtgactat
     1201 attttgattg gaaatcttta ccggtattac aacacaaggc atatttgcca acttgttaaa
     1261 gaggttatca atcaaaagga atattatcga gatatttata caaaccgcaa tgttgtcgaa
     1321 gcaaccctcc taaatgtggt agagacacta atagaacggc gcgctctaga agaagcaacg
     1381 ttttttctag agaaagttga agcactttta aataatgagc ggaatgctta tcaccgtatt
     1441 attttactct atgaaaaagg atttcttgcc tatgctaaag gggatagtcg aggaatacaa
     1501 agcatgaagc aagctatttt ttgcttccaa gcaattggtt ctaaacatca tgttgaaaat
     1561 tttcaggaac actttaatag ggtaacaaga ttataaaaaa tcccatttat gggatttttt
     1621 aatatttctc cttttggttt gctataatgt cgaactaagg agggaattat gaatattaat
     1681 ggtataaaat tactatctag tcgagcagtc agtaaacttg gtgatgtgct ttatgattat
     1741 ggaaatagta cttggattgc ttcgataggt ggtctaggac aaaagatttt aggtatttat
     1801 cagatagtag agttgttggt ttctattgtc ctcaaccctt ttggaggcgc cttagctgat
     1861 cgctttcaac gaagaaaaat tttactaata acagatgcta tctgtgctat catgtgtttc
     1921 ttactttctt ttattggaga tgataaagta atggtgtatg gtctaatagt tgctaatgct
     1981 atcttagcgg tttccaatgc tttttcttct ccggcttata aatcttatat tcctgaaata
     2041 gtagataaag ctgatatcat taccta
//
LOCUS       DATROY010000185         2058 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.186, whole genome shotgun sequence.
ACCESSION   DATROY010000185 DATROY010000000
VERSION     DATROY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.186"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..266
                     /locus_tag="VBD11_001948"
     CDS             <1..266
                     /locus_tag="VBD11_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954852.1"
                     /translation="NINNADIHKSVEIPWTDMVEQLNLIINTQLSTADKAIYSELLEI
                     SEAEDERKLKNFVNKMSSFFKNDYVQNTITGVLGNVISSILMK"
     gene            284..1582
                     /gene="purB"
                     /locus_tag="VBD11_001949"
     CDS             284..1582
                     /gene="purB"
                     /locus_tag="VBD11_001949"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6954853.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            1735..>2058
                     /locus_tag="VBD11_001950"
     CDS             1735..>2058
                     /locus_tag="VBD11_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6954854.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEE"
BASE COUNT          674 a          341 c          457 g          586 t
ORIGIN      
        1 ctaatattaa taatgccgat atacataaaa gtgtggaaat tccatggact gatatggttg
       61 agcagttaaa tttaattatt aacactcagc tatctactgc ggataaagcg atatattcag
      121 aattgttgga aattagtgag gcagaggatg aaagaaaatt gaaaaatttt gtcaataaaa
      181 tgtctagttt cttcaaaaat gattatgttc agaataccat aactggggtg ttaggcaatg
      241 tcatttctag tattttaatg aaataagtta aaggagacaa acaatgattt cacgttattc
      301 acgccctgag atggcggcaa tttggacaga ggaaaataaa taccgtgctt ggttggaggt
      361 cgagattttg gctgacgagg catgggctga gttgggtgag attcctaaag aggatgtggc
      421 taagattcgc gagaaggcgg attttgacat tgaccgcatt cttgagattg agcaggacac
      481 gcgtcacgat gtggtggctt tcactcgtgc ggtttctgag acgcttggtg aggagcgcaa
      541 gtgggtgcac tacggtttga cgtcgactga cgtggtggac actgcctacg gttacctcta
      601 caagcaggct aacgacatta tccgtcgtga tcttgagaat ttcacaaaca tcgtagctga
      661 caaggctaag gagcacaagt tcaccatcat gatgggtcgt acccacggtg ttcacgccga
      721 gccaacgact ttcggtctca agttggcgac ttggtacagc gagatgaaac gtaatattga
      781 gcgttttgaa catgctgccg caggtgtgga agctggtaag atttcaggtg ccgttggtaa
      841 ctttgccaac attcctccat ttgtggaaaa atatgtctgt gacaaattgg gcatccgtcc
      901 gcaagaaatt tcaacacagg ttcttccacg tgacctccac gcagaatatt ttgcagtact
      961 tgcaagcatc gcaacatcta tcgaacgtat ggcgacagag attcgtggtc tgcaaaaatc
     1021 agaacaacgt gaagttgaag aattctttgc caaaggtcag aaaggtagct ctgctatgcc
     1081 tcacaaacgc aacccaatcg gttcagagaa catgacaggg ctagcgcgcg tgattcgtgg
     1141 tcacatggtg acagcttatg agaacgtgtc actttggcat gagcgtgaca tttcgcactc
     1201 atcagctgag cgtatcatca caccagacac aactatcttg attgactaca tgctcaaccg
     1261 ttttggcaat atcgttaaga acttgactgt cttcccggaa aatatgatgc gcaatatgga
     1321 atcaactttt ggtttgattt atagtcaacg tgttatgctc aaattgattg aaaaaggaat
     1381 gacacgagaa gaagcttatg acttagttca acctaagaca gcttattcct gggacaatca
     1441 agtggatttc aaaccacttt tagaagaaga caccaaagtt acctcttgtc ttacacagga
     1501 agaaattgat gaactattta atccgattta ttacacaaaa cgtgttgatg atatttttga
     1561 aagactagga ttagaaaaat aaaaaaattc cattactggt taacgttttt ttaaccagta
     1621 atggaatttt tattgattta aaaggaatta atattaaaaa aacatactta aaatgagttt
     1681 attatgataa tatataataa gaagaaaaat ttaggagtta gtttttaaag gaaaatgtta
     1741 aaacattttg gtagtaaagt aagaaatttg agagtaacta gaaatattac tcgcgaagat
     1801 ttttgtggtg atgaaactga gttatctgta agacaattag ctaggattga aagtggacag
     1861 tcaatcccaa acttgactaa agctcactac atagctaaac aattgaatgt aaaattagat
     1921 attttaacag gaggagagag cttagaatta cctaaacggt ataaagagtt gaagtatttg
     1981 attttaagaa ttccaactta tgcagatgct gaacgattaa aattaagaga atgtcagttt
     2041 gatcatattt tcgaagaa
//
LOCUS       DATROY010000186         1963 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.187, whole genome shotgun sequence.
ACCESSION   DATROY010000186 DATROY010000000
VERSION     DATROY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.187"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..351
                     /locus_tag="VBD11_001951"
     CDS             <1..351
                     /locus_tag="VBD11_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954855.1"
                     /translation="LIDYINNNYQQNLTIEFLADYMGYSKTHFMTVFKQHTGTSCTEF
                     IIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQFKRYYHTTPRQYRKQFTNKK
                     DGSTLVYRNINTKK"
     gene            complement(408..1940)
                     /locus_tag="VBD11_001952"
     CDS             complement(408..1940)
                     /locus_tag="VBD11_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6954856.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
BASE COUNT          712 a          388 c          276 g          587 t
ORIGIN      
        1 ctgattgatt atattaataa taattaccag caaaatctta ccattgaatt tttagctgat
       61 tatatggggt atagtaagac acattttatg accgtattta aacagcatac tggaacatcc
      121 tgtacagaat ttatcataca agttcgtctt aataaagcta gtgagcatct tattaactcc
      181 actacggcca ttattgatat tgccaacagc gttggtttta ataatctatc aaattttaat
      241 cgccaattta aacgctatta tcatacaact cctcgccagt accgcaaaca atttaccaac
      301 aaaaaagacg gtagtacgct tgtctataga aatataaata caaaaaaata gaaactagtt
      361 aaatccatga ctagtttcta ttttttatta tttaagcgtt aattagttta catcagctgc
      421 tcacgtactt ctttaatttc cgattcattc acaactaaaa aaatggtgtc gccagcattc
      481 aagatagtat ttcctgatac tacagccgat gtcttatgat gaatttgagt cgttattagc
      541 acattttctg gtaattctaa atctcggaca tactttcctg atattttatc tgaaacagtt
      601 aattcaatca atgtaggtgt cattacttta tcttttggat tgaaggtcat tttagccaac
      661 atcgcttcat aaataggttc tccctttaat aaatccatga caatataaga taccattgtt
      721 acaattccaa tagccattaa ttgttttaaa tcgccaacca tttctgtaac caaaatcata
      781 gctgttagtg gtgcttttga gattgcacca aaatagcccg ccattcctag aacaacaaat
      841 aaagatatct gctgatgaga tattatacca aagtactgca tacctactgc aaaaaaagca
      901 cctgctaaag atcctaaagc tagaattggt aaaaaaattc ctcctggcaa gccactactg
      961 taactcaaca tactccataa aaaacgaatg aggaaaaata atcctaacat catcagacta
     1021 agattactcc taggcagtga tacaattaag ccatttccac cccccaataa ttgtgggaaa
     1081 tagtaaccaa taggaagaat aaaaattacc gctaatatac catataggtg agatggtaga
     1141 tgaaatagtt tacctaaata atcatagatc acatgaaatc tcaaaattac ccactcatag
     1201 ataaagccta agatacccaa aaagagaccc atgagtaaaa aaatccaata aaagttgaga
     1261 tttaaagatg gtaattctga tggtaaagct aaaactgggg ttaaaccaaa gatattaagt
     1321 gagacaaagt ttgccactaa acttgctacc aaagctgtta tccaaactaa gcgagaaaaa
     1381 tgatggtaaa tttcttcaac tacaaataaa aggcctgcaa taggagcatt gaaggctgct
     1441 gaaagacctg ctgctgcacc actagcaatt aaaacacgtt tttccattcg actagcattg
     1501 agatactgtg caattccctt acctgtcata gctcctaact gaatactggg accttcgcga
     1561 ccaagcataa gtcccgaaga aatagctaaa atacccgcaa tgaatttctt ccatacaata
     1621 ctaaaccagt ctggcgatag cattccttta agttcacctt caacatgcgg gatacctgaa
     1681 cctttaatat caggatcaga tttgataaaa aaacctatta tcactgcagc gataatacta
     1741 gtaaccgtaa tgctcaataa aataattggt tgataatggg cacttttata aagttccata
     1801 acaactaaaa aaattttttc aatcgctaat cgaaatatgc tgacaacaaa tcctgctatt
     1861 aagcctacta ctattcctct ccaaacaaat cccagaattg attcaagtga aaactgaaat
     1921 tcatttttat gattttccat attttcctcc ttttcttaca att
//
LOCUS       DATROY010000187         1952 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.188, whole genome shotgun sequence.
ACCESSION   DATROY010000187 DATROY010000000
VERSION     DATROY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.188"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            7..594
                     /locus_tag="VBD11_001953"
     CDS             7..594
                     /locus_tag="VBD11_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954857.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            540..1220
                     /locus_tag="VBD11_001954"
     CDS             540..1220
                     /locus_tag="VBD11_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954858.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            1205..1591
                     /locus_tag="VBD11_001955"
     CDS             1205..1591
                     /locus_tag="VBD11_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954859.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            1625..1906
                     /locus_tag="VBD11_001956"
     CDS             1625..1906
                     /locus_tag="VBD11_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954860.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
BASE COUNT          784 a          257 c          371 g          540 t
ORIGIN      
        1 aagataatga catattctta tagcgagtat gatttaaata gattgaatca aggtgagaac
       61 gtatatagtg taaatccaaa ctatgtgaaa caaaatggcg gaaaaataat aacagctaat
      121 ccaaaaaaat ctaatgaaac aaatttaata gtaacaaaga cgggtaaagc gtttaaggta
      181 ttaaaaactt ctcctgacga tatgtctggc tatcagggta tggcagttgc accaattatc
      241 aagggtaaag tagattataa cagtgttgca gttatttccg ccgcaacgga ttcaagtaat
      301 tataaagatt taattggcgc agtttcatct gctcaaccac atcaaagttc gactcagtta
      361 aaatcagctg ataaattttt aaaagacgta cagtcacatg acaaatggac tgtgactcag
      421 ctttcaggct attctcaatc tgcttatatg ctaaaattag gtgctaaata tcatatcccg
      481 actacagtat ttaatggatg gtttaggtat agcactttaa atgaagatga aaaaaattca
      541 tggctaagca tcctgaatat tttgctaatt ttcgacataa agaggataat gtaacttggt
      601 ggaacgattt taataaacta gatgataaag gttatggtac cgtgaaatgg gtaaatggta
      661 aaagtcataa aatagagagt tggaaattta ctgatgatgg tcagttaaaa gatgaaaaag
      721 gtaacatcgt aaatcctaag tcaccagctg ttcagtctgt tctttatgaa gaagttcatt
      781 ttcaaaaagc taaagctaaa ttaaaaaaat ctggaggcaa acttagtcat agtgaaaaag
      841 tatatttaga ttctgaacag gctatattta ttgctaacgg tttaaccacg gcatctcaaa
      901 cagcttctga tgatatcaag aagaatgctg aattagctaa agaaaaagca tcagaactat
      961 ttgcaaaaac taaagtgatg cctcctggta taacagattt aagtccagaa gaattagctg
     1021 atgcatatag tgcgggtgga gttagagagg acaccatagt aacaccaatt gaaactttct
     1081 ttgatgaaaa agttactaat gcacaagaga tcacaacttc atatactaat cttcaaaaac
     1141 aaattgaatc tggtgtccaa aaattgctag aagaggactc caaattagca ggagaattta
     1201 aggaatggtc acaatactag atcaaaatga aaacaaaatt aatagtctaa aaataaacct
     1261 agggaaattg gaagataatt atcatcgcga aaatatggag atagaagata aattatatag
     1321 ccttgaggat aaaaagaacg agttagagac ttatttacaa gaagtctatg aagagacaag
     1381 tcactttttg agacaaaatg attcagatgg ttcgactttt atgagtttaa atcgaataat
     1441 tgaaagttat caaatggaat taattgatga atatgaaaac tataaacaga ctatcattgt
     1501 ccaagaggaa aatagtcgga cacagtttct aaaagaccga gtaaaattag aaaatgacat
     1561 ttctagttta gaaggaagaa aatactacta atttagatgg actataaagt gaaagaggca
     1621 ataaatgagt ttaataggcg ctgtattgaa aggaaccaaa gaagacaaag aattcaaaaa
     1681 gcaaattcaa aaagctttag taaggtttgt ccatgccccg gaaatagtag aagaagctga
     1741 ttctattaag acgacttata gtgaaaagat tgatgatatt tctgagactg ctaaatcatc
     1801 aataaaaacg ataaaaaacc aaattgacac tggacttgta ggacaatttt gggaaaaagt
     1861 aaaggcacag gtagatttaa ataaaggtag ttatgatgat ttataatcga gtgaaaatgt
     1921 tgaggaagct tactatcctg atagttatta ta
//
LOCUS       DATROY010000188         1926 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.189, whole genome shotgun sequence.
ACCESSION   DATROY010000188 DATROY010000000
VERSION     DATROY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.189"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(586..804)
                     /locus_tag="VBD11_001957"
                     /pseudo
     CDS             complement(586..804)
                     /locus_tag="VBD11_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1692..>1926)
                     /locus_tag="VBD11_001958"
     CDS             complement(1692..>1926)
                     /locus_tag="VBD11_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954861.1"
                     /translation="KKWKFNQWKLQKGKKEQLKINYGIIFGLVMLSPVFLVGSIENIF
                     GLIGGVLFTATFTALIVDSLYAAYYIHKYPEYE"
BASE COUNT          637 a          350 c          224 g          715 t
ORIGIN      
        1 aaaatgtctc tctatcaagt attctcaaga caattaaggg gatatccatc aacaactatt
       61 atgaaaacaa ctacttaagg atgatagata ctttcactaa aaaattttga ttaatcaaat
      121 atattattat tatagcatat caaatattat tttttacata tttattaatt aatatggtaa
      181 cacccattat ctgaagtgtg taggcgaggt ctaagtaaag aatttaagcg ttttgataat
      241 aaaaaagtga gaacaataga ttctcactca caattagcct ggtaatttct cacacgattt
      301 ggtactgctt tttttaagtt gggcgatggt gttattttta atatgagtag ctgcactcga
      361 atccgattgc tcaaaagcac ttgtcgctcc attgagatag ctattcagat cagaaataac
      421 tggttgaatg ttttggctga cttctttgat aacttttgat ccagcttcta agtattttca
      481 atgttcttga acccattggt gatctttttt tactaagtca catgctgtta aaatcatcat
      541 taagcttatc ccttccacaa taataatcaa ttttttaaat cctttttatc ttattgattt
      601 tttcaatttt attgctgtct tgacgttgct atcgcaaaca accaactcaa tgcaatatag
      661 ttacggtata catatcaatg taagactcaa gatttttaaa ttcatattat ttgtaggttc
      721 tttatcagac caagctaata ctctttcatt tgaagtaata ttattatttg tatgatattc
      781 aagattagct atttttgatt tcacaaaaaa accttcttct acttttttag actgattctt
      841 tcttcccact tcttcgtatc aatatcatat atttgaagaa ctaaactctc agagtttcca
      901 gtcaatttta gtaataatcc caaaactaat ccttcctaca aaccaacgga taattttatt
      961 tcaactggtc ctggttttaa ttttacttgt gcaatttcgc ttgttatacc aactattgat
     1021 atcgccgata ctcctattcc taatgatgcg cacgcatcat tagttaaaca aagtacttta
     1081 aatcctacag agggtcattc catcaattaa tggcataggt tttggcacta gaaactgaat
     1141 atgaattcta ttatctatta aaaaaatcaa tatcaccaga gtatttataa aacataacga
     1201 taaaaaatca aaaaatattt ttactatttc ccttaatttc atgagattta atttcccttt
     1261 ttgtgtatta tttctattct acaatagtct tacccttaga aatatttttc catttttcaa
     1321 tgatacgagc tactctttct tactcctcgt catggtacct cccaaaatta cttcatcaat
     1381 atcttctcat tgaaataata tacttgttct aaattgacct ctctggaaaa atcctgccag
     1441 ataatcaaaa tataatcttg atcaataata tttgctcatg aaacggctcc acaaaatttc
     1501 ttacatcttt aacattttca tagagtgctt ttactccaca atggccaaag tacctataac
     1561 aactgctact actggtacaa attggagttg ttatgattac tacaaaaaca ctctcagcaa
     1621 gcgtagagac tattctaccc acttcatttt ctctagatat ttattgctcc taccttaaat
     1681 taattaattt atcactcata ttcgggatat ttatgaatat aatatgctgc ataaagggaa
     1741 tcgacaatca gagctgtaaa agttgccgta aataatacac ctccaatcaa accaaaaata
     1801 ttttcaatac tacctacaag aaagactgga gaaagcatca ctaaaccaaa aataattcca
     1861 taattaattt ttaattgttc cttttttcct ttttgtaatt tccactgatt aaatttccat
     1921 tttttc
//
LOCUS       DATROY010000189         1887 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.190, whole genome shotgun sequence.
ACCESSION   DATROY010000189 DATROY010000000
VERSION     DATROY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.190"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..264
                     /locus_tag="VBD11_001959"
     CDS             <1..264
                     /locus_tag="VBD11_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954862.1"
                     /translation="SLYNQLINSEPIGFIDPLTDLGEFDIFQMKFKEPVRYLKNKYSG
                     KPYSPHWQNKIEKMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            406..684
                     /locus_tag="VBD11_001960"
     CDS             406..684
                     /locus_tag="VBD11_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6954863.1"
                     /translation="MTGTQNLNANFEDEKSSMVYYENMVAHYIRLTNHHVRYRVTLLF
                     KNIEMVTRGVGMETQSVEDNQISFDIYIFNVQSGYNINYLTRYSVKEN"
     gene            694..894
                     /locus_tag="VBD11_001961"
     CDS             694..894
                     /locus_tag="VBD11_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954864.1"
                     /translation="MNSEIRNRKHVYLNDDIIFQLAEISEFLKSSQSTFKQERSPYSQ
                     IIDLRKEEAQAKSVRNSIEKLL"
     gene            1025..>1887
                     /locus_tag="VBD11_001962"
     CDS             1025..>1887
                     /locus_tag="VBD11_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO6954865.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MTLHNDVKDIM"
BASE COUNT          766 a          221 c          351 g          549 t
ORIGIN      
        1 agtttatata atcagttaat aaatagtgaa ccgataggct ttattgatcc actaaccgac
       61 ttgggggaat ttgatatttt tcaaatgaag ttcaaagaac ccgttagata cttgaaaaat
      121 aaatattcag gaaagcctta ttcaccacat tggcagaata aaattgaaaa aatgaggtct
      181 catcaagaaa gcctaagaga agaagttaaa gaagagattc cgattactag tagggcaaga
      241 aatcaagaaa ataatttgag ttgatcttaa attgataaaa agttcgactt ttttcctaaa
      301 aaatagtgtg ctataagtca attaaaagat gtgctatatt tatttaatag atatcgtctc
      361 attgaatatc aactaaccgg agaaaattcg aatgcaaaaa atttaatgac aggcactcaa
      421 aatttaaatg ctaattttga agatgaaaaa tcgtcgatgg tatattatga aaatatggtg
      481 gctcattata taagactaac aaaccatcat gtgagatacc gtgttacact cttatttaaa
      541 aatattgaga tggttacaag aggggttggt atggaaaccc aaagtgtgga agataatcag
      601 atatcttttg atatttatat ttttaatgtg cagtcaggat acaacattaa ttatttaaca
      661 agatattccg ttaaggaaaa ttaaaggaga aaaatgaatt cagaaattcg aaatagaaaa
      721 catgtttatt tgaatgatga tattatcttt cagttagctg aaatctcaga atttttaaag
      781 tcaagtcagt caacatttaa acaggagcgt agtccatatt ctcaaataat agacctacga
      841 aaagaagaag cacaagctaa aagtgttagg aatagcatag aaaaattatt gtaagaaaac
      901 agtgatctaa tacaggtaag gaagtatgag ttaggctata aaactaagta aaaaaatatg
      961 atataatata aaaaattatc gagtaaaata aactggaagc gaaacttaag gagatgatta
     1021 ttaaatgaaa atatgtattt taggattagg cgtaataggc acaacttatg gatatatctt
     1081 tcaaaaagca ggacatcagg ttgaacattt agttagagaa agtaaaaagc aagatgttcc
     1141 attaaagctg gatataacca tgttagatgg gcgatataac aataagggag aagaaaaaac
     1201 agatatatat actgtgaatc ttgcaaaacc aaatacaaat tatgacttta tccttttaag
     1261 tgttgcaggc gggaggataa aagatgccat agcaacactt tcacaaaata atattacagg
     1321 aagtttgtta ttattttgta acttttggaa cagccgtgaa gaaatcgaaa aaatggttgg
     1381 tgattttaaa tatatcattg gatttccgac agcaggtggg aatttgaagc agaaaaagtt
     1441 gaattgtgtt ctgtttgacc atataatgct tgaaagtgaa gaaaaagcaa ctatcacaaa
     1501 ctataaagag ttgttagaat ttctcgaatc tgccgacata aaagtagaaa atccttatga
     1561 tatgatagag tggatatgga ttcatatggc aataaatgca ggtgttactt ctactgcagc
     1621 aagggcagga aaaattgatg atccaaaaca acttgcggtt aatcttatga gagataatca
     1681 tggattgtct atcgcggtaa aatcaataag agaaacaatt aaagtagtaa aaaatagagg
     1741 ggtcgattta agattatata aaaatgaact ttttccttat aaaatacctt ctactattgc
     1801 aggaataatt atgaaacgaa tgttcgcaag aaatgaactt acgagtagaa ttatgacgtt
     1861 gcacaatgat gtaaaagata ttatgta
//
LOCUS       DATROY010000190         1886 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.191, whole genome shotgun sequence.
ACCESSION   DATROY010000190 DATROY010000000
VERSION     DATROY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.191"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            134..940
                     /locus_tag="VBD11_001963"
     CDS             134..940
                     /locus_tag="VBD11_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6954866.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELYVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1023..1526
                     /locus_tag="VBD11_001964"
     CDS             1023..1526
                     /locus_tag="VBD11_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6954867.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1630..1806
                     /locus_tag="VBD11_001965"
     CDS             1630..1806
                     /locus_tag="VBD11_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6954868.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          688 a          259 c          366 g          573 t
ORIGIN      
        1 agttgttatt aaaaatcgtc taacagcaag catagttatc ggtgtaattt actgaatttt
       61 tattcaaaaa aacttgactt atgtataaag atgatttata cttgttccta tcataataaa
      121 aggaagatag tttatgtcaa aaaaaataat attaggaatt ttatctcttt tatctgtcgt
      181 tactttggtg gcgtgtggtt catcagacaa acagctacaa gataaagttg agaaaaaagg
      241 gaagttagtt ttagcggtga gtccagatta tgctcccttt gagtttaaag cgcttgttaa
      301 tgggaaagat aaagttgtcg gcgcagatat tgagttagct caagctattg ctgatgagct
      361 ttatgtcaaa ctagaggtat ctcctatgag ttttgacaat gttttatcaa gtttacaaac
      421 aggtaaggct gatatggcta tttctggatt atcatatact aaagagcgtg ctaaagtcta
      481 tgatttttca aaaccttatt acactacaga aaatgcggtt cttgttagga agtctgattt
      541 aggaaaatat acttcaatcg aaagccttaa aggtaaaaaa atagcagtac aaaaaggaag
      601 tatcgaagag ggagtttcta agaaatcatt caaaaattct cacgtcgtgt cactgacagc
      661 gatgggagaa gcgattagtg agttaaaatc aggcaaggta gaagctgttg atttagaaaa
      721 acccgtagct gaaggttact tggcccagaa tccagacctt gttcttgcta agtttgccct
      781 taaaacagga gaaggggatg ctaaggctgt agctttacct aaagacagtg gaaaaatgtt
      841 gaagactgtt aacaaagtga taaagagact tgaaaaagaa gataaatata aagtttatat
      901 ttcagatgcg gctaaattaa ctggcaatca agttgaataa tattaaaaag cctagtcata
      961 ggcttttttg gatgttataa tatggcttga ctaagaataa tagcataaag gcagaggaat
     1021 atatggttca gggttcaatt aatattgaaa aatataaaga tttggcgctc caaaaacaag
     1081 gcgagcatcg taaattttta gcaagtttaa aaaagaaagc tcctaaaaac ttagataaaa
     1141 tagtccaaca gatacatcaa gaggtttttc aagaaattga ttgtacagca tgtgctaatt
     1201 gttgtaaaac tttgggtcct ctatttacag aagctgatat ttctaggatt tctaaacatt
     1261 tccggatgaa gttatcggct tttgaagatt tatttttaga agtagatgaa gatggagata
     1321 aagtatttaa agcgatgcct tgtccgtttt taggcgatga taacttatgt agtatatacg
     1381 atgtcagacc taaagcttgt cgtgaatttc cacatactga tcgtaagaaa atctatcaaa
     1441 ttaataactt aacgataaaa aatactctta cttgtccagc ggcatacctt tttgtggaaa
     1501 aattgaaatc acgtataggg aaataataat ccagagaaag tatcataaaa atattgacaa
     1561 tctgctttta tttgggtatc ctagtaagtg aacgtgaaaa tgttacaata tcttgaggag
     1621 gtgaaacata tgtcaaaaac agtagtacgt aaaaacgaat cacttgacga tgcacttcgt
     1681 cgtttcaaac gttctgtgac taaagctggt actcttcaag aatcacgtaa acgtgaattc
     1741 tacgaaaaac cttctgtaaa acgtaaacgt aaatcagaag cagctcgcaa acgtaaaaaa
     1801 ttctaattag atagaatatg aaaaaacagt tcagaattga gctgtttttt catatattat
     1861 aactataagt taataaaaaa actcgg
//
LOCUS       DATROY010000191         1881 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.192, whole genome shotgun sequence.
ACCESSION   DATROY010000191 DATROY010000000
VERSION     DATROY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.192"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            61..1143
                     /locus_tag="VBD11_001966"
     CDS             61..1143
                     /locus_tag="VBD11_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6954869.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(1175..>1881)
                     /locus_tag="VBD11_001967"
     CDS             complement(1175..>1881)
                     /locus_tag="VBD11_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6954870.1"
                     /translation="GRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYLKSSDLDLVS
                     NLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFIMGQSAVGHTDILQRMHAE
                     GHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYGATNATVKTV
                     TGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDALPTIMDYLT
                     TQGYYFVTVGELYSTP"
BASE COUNT          631 a          343 c          299 g          608 t
ORIGIN      
        1 taaaaataaa aagcatgtta taataacaat gacaacatat aaaataacaa gaggattctt
       61 atgataacaa aatttccaga ccaatggcag gataaattaa cccagcgtca atttgatgat
      121 ctaacagata ttcaaaataa actttttcaa cctattactg atggtgataa tatccttgga
      181 attagcccta caggtactgg taaaactcta gcataccttt tcccaacgct tcttaagttg
      241 caacctaaaa agagccaaca actcttaatt ttagctccaa attcagaatt agctggacaa
      301 atcttcgacg ttacaaaaga atgggctgaa ccattaggac tgacagcaca actattcctt
      361 tcagggtcta gtcaaaaacg ccaaattgag cgactaaaaa aaggaccaga aatccttata
      421 ggaactgctg gacgcgtatt tgaacttgtg aaactaaaaa aaataaaaat gatgaacatt
      481 aatactatcg tccttgatga gttcgatgaa ttactcggcg actcacaata tcattttgtg
      541 gacaatatta ttaatcgcgt tcctcgtgat cagcaaatga tttacattag tgccaccaac
      601 aaactggaca atagtaaact tgccgataac actataacaa ttgacctatc tgatcaaaaa
      661 cttgatacta taaaacatta ttatattaca gttgataaaa gagagcgtac tgacttactt
      721 cgtaaatttt caaatattcc tgattttaga ggtcttgtct tttttaacag cttatcagac
      781 ttaggtgcat gtgaagaacg tttacaattt aatagggcct cggcagtttc tttagcaagc
      841 gatattaata ttaaattccg taaagttatc ttagagaaat ttaaaaacca tgatatttca
      901 ttactcctag gaactgattt ggtagctcgt ggcattgata tcgacaacct agaatatgtg
      961 attaattttg atatcgcacg cgacaaagaa acatatacac atcgttctgg acgaactggt
     1021 cgtatgggga aagagggatg tgttattaca tttgtaacac ataaagaaga gctcaaacaa
     1081 cttaaaaaat acgccaccgt tgcagaactt gtacttcaca atcaaaaatt acacttaaaa
     1141 taaaaaagaa acaagtaact tgtttctttt tttattaagg cgttgaatat aattcaccta
     1201 cagtaacaaa ataataaccc tgggttgtca gataatccat aattgtaggc aaagcatcta
     1261 tggatgtctg gtgaatatca tgcatcaaaa tcactccccc aggacgtaat tgttttttga
     1321 tattggtcat cattgcttgt gtattatgat ttttccagtc ttctgtatca actgaccaca
     1381 acatttcttt caatcctgtc actgttttta cagtagcatt tgttgctccg taagggggac
     1441 gtaagtacac tggtttttgt cctgttgctt tcatgattag atcattagtc ttatgaatct
     1501 cttccttgat tttctccgct ggtagtttgg ttaaattagg atgatcccat gtatgattac
     1561 caatctcatg accttcagca tgcatccttt gtaaaatatc tgtgtgccct acagcacttt
     1621 gtcccattat aaagaaagtc gctttagcat tatatctttt aaggatttct aaggctttag
     1681 gagtcgtttt ttcatttggt ccatcatcaa aggtaagtgc aacacgtggt gctttagctt
     1741 ttaagttaga aactaaatct agatcacttg attttaaata tgactcatcg atcacattat
     1801 ataatttatc aagtggtaat tttaaatcat taataattaa cttaccatct ttaacatcta
     1861 gcccatctgt tactcttcca g
//
LOCUS       DATROY010000192         1850 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.193, whole genome shotgun sequence.
ACCESSION   DATROY010000192 DATROY010000000
VERSION     DATROY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.193"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            32..250
                     /locus_tag="VBD11_001968"
     CDS             32..250
                     /locus_tag="VBD11_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO6954871.1"
                     /translation="MKIGFPENTAREIIKQAKAIAVQRFENNIQETGKVIQLGQSPFD
                     NVRLNLAPASIVEELLGFELPLLEKEGA"
     gene            253..1485
                     /locus_tag="VBD11_001969"
     CDS             253..1485
                     /locus_tag="VBD11_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6954872.1"
                     /translation="MASKKYSGVSRDKKNRIYFQTEFGKDPVTGKRRRKKSYKDKNGN
                     PFRTEKQAYDELCRVRAEVRSQDELAQKQTVPVLTLEDFINTVFLPYYIKTVESSTYK
                     TALTHFDIFKEELGQYLLSDISVQFCERFRLSLQDKYSPNYAKNVWLRFKKCLTYAER
                     LEYIEFNPCTKLDNIKGERTVTSFWTVEDFQKAVNAFDRTTYQGVYYYTVIWLYFMTG
                     LRVSEGLSLIWSDIDFESKLLHVQSTLEYDENGNPFRKNKTKTEAGTRYVELDDMTLQ
                     TLKEWKEVQIHNSLGDFVLAREEVPMHKTTLTRLLKRVAKEQDLPVMAGKGLRHSHDS
                     FLINVLHKDVLYVSARSGRTDKATTLNTYAHIYAKKKAEGGREITKALESFGFDNPTK
                     TPPSKEEIAETLEITRFN"
     gene            complement(1646..>1850)
                     /locus_tag="VBD11_001970"
     CDS             complement(1646..>1850)
                     /locus_tag="VBD11_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954873.1"
                     /translation="IFYWSCYNENIREQIKSQQVHAVHKLIEKYGDTFNNKNHGRNLF
                     TKLQLENRLIIQEILYNDILNPY"
BASE COUNT          679 a          275 c          339 g          557 t
ORIGIN      
        1 tacaaaagta acagtaaaac atattgactt aatgaaaatc ggattcccag agaatacagc
       61 tagagaaatt ataaagcaag caaaagcgat tgcagtacag agatttgaaa ataatataca
      121 ggaaacagga aaagtgatac aattaggaca atcaccattt gataatgtga gattaaacct
      181 agccccagct agtatagttg aggaattgct tggctttgag ttgcctttac tagaaaagga
      241 aggtgcttaa agatggcaag taaaaaatat agcggagtat ccagagataa gaaaaataga
      301 atttactttc aaacagaatt tgggaaagac cctgtaacgg gaaaacgaag acgtaagaag
      361 tcctataagg ataaaaatgg aaatccattc cgtactgaaa aacaggctta tgatgaactt
      421 tgtcgagtta gagccgaagt caggtcacaa gatgaacttg cacaaaaaca gacagttcca
      481 gtattaacgt tagaagattt tattaacacg gtatttttac cgtattatat caaaactgtt
      541 gaatcgtcta cctataaaac agcattgaca cactttgata tttttaaaga agaactaggg
      601 cagtacttac tttcagatat atctgttcag ttttgtgaac ggttccgtct ttcattacaa
      661 gataagtatt caccaaacta tgcaaaaaat gtttggctta gatttaaaaa atgcttaact
      721 tacgcagagc gtttggaata tattgaattt aacccttgta ctaaactaga caatatcaaa
      781 ggtgagagaa cagtgacttc attttggacg gtagaagatt ttcagaaagc agtcaatgca
      841 tttgatagaa caacatatca aggtgtttat tactatacag taatctggct atattttatg
      901 acaggattac gagtttctga aggattgagc ctaatttggt ctgatattga ttttgaatct
      961 aaattattgc acgtgcaatc aactttagaa tatgatgaaa atgggaatcc atttcgtaaa
     1021 aacaagacta agaccgaagc aggtacgaga tatgttgaat tagatgatat gacacttcaa
     1081 acgctaaaag agtggaaaga agtacagatt cataatagtt taggtgattt tgttttagct
     1141 cgtgaagaag ttcctatgca taaaactacc ctaactcgtc ttttgaaacg tgtagcaaaa
     1201 gagcaagatt taccagttat ggctggtaaa ggtcttagac attcgcatga tagctttttg
     1261 attaatgttc ttcacaaaga tgttttatat gtatcagccc gttctggaag aacagataaa
     1321 gctacaacac tcaatacata tgcccatatt tacgctaaga aaaaagctga aggtggtaga
     1381 gaaataacga aggctttaga atcgtttgga tttgataacc ccaccaagac cccaccaagc
     1441 aaagaagaga tagcggaaac acttgaaata acaaggttta attgaaaaac gcaactgtta
     1501 ctcttaaata atcatctatt ggttgtttaa aggtgaaagc ctagttttaa agtcttttcg
     1561 ttaaagttaa ggcgattaaa aaaggaaaga ttcaattgaa tcttccccac aaacaccccc
     1621 acaatatttt gtgggggtgt tttgtttaat atggatttag aatatcatta taaagtattt
     1681 cttgaataat taaacgattt tctaattgaa gttttgtgaa taaatttctg ccatgattct
     1741 tattattaaa agtatctcca tatttttcaa ttaatttatg tactgcatga acttgttgag
     1801 atttaatttg ttccctaata ttctcgttat aacaactcca atagaaaatt
//
LOCUS       DATROY010000193         1817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.194, whole genome shotgun sequence.
ACCESSION   DATROY010000193 DATROY010000000
VERSION     DATROY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.194"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            209..880
                     /locus_tag="VBD11_001971"
     CDS             209..880
                     /locus_tag="VBD11_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6954874.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
     gene            965..1636
                     /locus_tag="VBD11_001972"
     CDS             965..1636
                     /locus_tag="VBD11_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6954875.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            1620..>1817
                     /locus_tag="VBD11_001973"
     CDS             1620..>1817
                     /locus_tag="VBD11_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6954876.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALA"
BASE COUNT          696 a          295 c          287 g          539 t
ORIGIN      
        1 ctttttattt ttaaaaataa aatctgacaa aaaagtataa atttacctct taccttcagg
       61 tagttagtca ttcttttaaa actcactttt ttataactga aaatattgtt cttaatttta
      121 aaaattcatt ttagattcat tttacgacta tatactcaga agtaacaaac ctaatccaag
      181 gtttgaaaaa agaaagaagg aagtcagtat gacaaactat aaaaacaact ttaaaaaatt
      241 catgagcaat aaattatacg tagggttagc ttccattacg ctacttggta ttgggggagc
      301 tataggagta agtggagctg cattagcgca taaccatcac tcagaacaca tgtcttttga
      361 acataagcat gaaggacacg ataaacatca cgatcataga gaaaaatact cactaaaaac
      421 attaaagaat atctcgacaa aaatcgatta taagactgcc ttagaaactg ccaaaaaaga
      481 agctcgctca ggctttattt cacaaattga attaaatacc aaaggtgata caccttttta
      541 tgaaatcaaa gttattgata ataaaatagc taaaaaatat aaaattgatg ctaattctgg
      601 gaagatcctt tcaacaacat cagataatca actcaattca aaagatgccc aattaaaaca
      661 accaagtaaa gatattcaga ctgcattaca agctattcaa gcaaaatata aaaatgcaaa
      721 aataaaagaa atttctcttg cgaataacaa atcaaataag ctagtttata aagtaaaact
      781 acttgatgga aatactccta agattgccca atttgatagt gataaagcat catttataaa
      841 ggcaaataag cttaatacaa ctcataaaaa tcacgactaa tactagtgta ttaaagggaa
      901 taggacctca gtactccatt cccttttgtg atataatgga aaaaagccat acggaggcca
      961 aaaaatgaaa atccttgttg ttgaagatga gtttgacttg aaccgtagta ttgtaaaact
     1021 tttaaaaaag caacactaca gtgttgacag tgcttcaaat ggtgaagaag ccttacaatt
     1081 tgtttcagtg gcagaatatg atgtgattat cttagatgta atgatgccaa aaatggacgg
     1141 cttcaccttt ttaaaactgc ttcgcaacaa aggaagtcaa gtatctattc ttatgttgac
     1201 tgctcgagat gccgttgaag atcgtatcgc aggtctagat tttggtgcgg atgattacct
     1261 tgtaaaacca tttgaatttg gagaactcat ggcccgaatt cgagctatgt tacgacgtac
     1321 aaatagacaa gtatcttctg atgatattca aattcaagat ataacaatca atttatctac
     1381 aaagcaagtt tggagaaacg acaatttgat tgatttaaca gctaaggaat acgaagtcct
     1441 tgagtatttg gcacgtcaca gagaccaagt cctctctcgt catcaaattc gtgaacacgt
     1501 ttgggattat gattattatg gagagtccaa tattattgat gtccttatca aaaaccttcg
     1561 tcgaaaacta gataacaatc gagatggatc actaataaaa actaaacgag gtttaggata
     1621 tgttattcca aagtaaaata aattacccta aaaagtctag catcatcact caaattaccc
     1681 tctggtacag ctcttttatt tttatccttg ttatcggtgt attaattggt tcatttttta
     1741 tttcaaaaag cattgctgaa aacaaatcta aaaaaaattt agaagctaaa gctgtgcaaa
     1801 tgagtcaagc gttagct
//
LOCUS       DATROY010000194         1709 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.195, whole genome shotgun sequence.
ACCESSION   DATROY010000194 DATROY010000000
VERSION     DATROY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.195"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..143
                     /locus_tag="VBD11_001974"
     CDS             <1..143
                     /locus_tag="VBD11_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6954877.1"
                     /translation="LIIISLVKLSKLKMTKHLSKPVWFLVIIFLNIIGPIAFLILEGN
                     NE"
     gene            140..1042
                     /locus_tag="VBD11_001975"
     CDS             140..1042
                     /locus_tag="VBD11_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954878.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            1039..>1709
                     /locus_tag="VBD11_001976"
     CDS             1039..>1709
                     /locus_tag="VBD11_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954879.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKV"
BASE COUNT          581 a          239 c          295 g          594 t
ORIGIN      
        1 ctttaattat catttctcta gtcaaactaa gtaaacttaa gatgactaaa cacctttcaa
       61 agccagtttg gtttttggtc attatatttc ttaatataat tgggccaatt gcatttctta
      121 ttttggaagg gaataatgaa tgaaagctat ctcatttcaa aacgttacaa aatcttttgg
      181 tcctaaaaaa atattaaata atgtttcttt tgatttagaa gaaaatatga tttatggatt
      241 cgtaggacca aatggagcag gtaaaacaac gacaataaaa atgattttag gattgttaaa
      301 atttgatacg ggtttcataa caatttttgg taaaaaagtt aattttggaa gaacagatac
      361 caatcaactg attggctatc tcccagatgt tcccgagtac tacgattata tgacagctct
      421 agaatatctt gatttatgtt ctggtttagc gagatcaaag cataagcttt ctaacaaaga
      481 actacttagg tcagttggac ttgacgataa tcaccaaaag attgctactt attctcgagg
      541 aatgaaacaa cgtcttggat tagcccaagc actggtccat gatcctaaaa ttattatttg
      601 cgatgaacca acctctgcat tagacccgaa aggtcgccaa gatattttag atattatttc
      661 aaatcttaga ggagaaaaaa ctgtgatttt ttctacccat attctctctg atgtcgaaaa
      721 aatatgtgat cacgttctgg tgttaactaa gtgtgggatt tattctcttg aagagttaaa
      781 aggaaaaaaa tcagaggaaa attattcggt tcgtatttta ataaaagtga caaagtctga
      841 agctaaggta ctatctcata actatcagat agaaaagaaa gataatgagt atgcactgac
      901 tctcaaaggc tctaaaatgg ataacaaagc agatttacta gctggttttt accaagattt
      961 agtttcttta aagattagcc catcagctat tgaagtcatt gacaatagtt tagaggagct
     1021 ttatttggag gttaccaaat gaaatcttta gcagtaatgt taaaaaaaga atggatggag
     1081 aatgttagga catacaaagt tatatctatt ctgattactt gttcaatatt tggtatatta
     1141 ggtcccttaa ctgcacttat gatgcctgat attatggcag gaattttacc taaaaaacta
     1201 cagggagcga taccagagac tacctatatt gattcttata ttcagtattt taaaaatatg
     1261 aatcagcttg gtttagttat tttagtattt ttatttagta gtacactgac gcaagaattt
     1321 tcaaaaggaa ccttaattaa tctagttacg aagggattag ccaaaaaagt tataatcctt
     1381 gctaaattta tagtaattac ccttctatgg acagtgtcat acttattaag tgttgtcatc
     1441 cattttagtt atacgttata ttactttagt aacgaaggaa gtcataaatt aatggtttac
     1501 ggtgcaacat ggtttatcgg tattcttttc ataagcttaa tactcttttt ttcagttctt
     1561 tttcgtaaaa ctttagggat tttgttaggt tgtcttttta tggtggtgtt atgtgtagta
     1621 tctggatttt ttaaaatcag taaaagctat aatcctatgc ttcttatcca aaaacaagga
     1681 gaaatgttaa gtggaaagtt taaagttca
//
LOCUS       DATROY010000195         1700 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.196, whole genome shotgun sequence.
ACCESSION   DATROY010000195 DATROY010000000
VERSION     DATROY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.196"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(32..505)
                     /locus_tag="VBD11_001977"
     CDS             complement(32..505)
                     /locus_tag="VBD11_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954880.1"
                     /translation="MIAIIRTREKGRSQFLCELDIMQFTENQVRNRMIEKGIKDDAFF
                     ICGFSDWNVDRIMSLTEVYLLKRCIEGLYDGDDYIVQYLLKKGLSVHSIVTKYYIFLS
                     NNENKVMKYILRKVEFDSLIDFWQRSVTWVNALNAYIEEGIVLNTSKGFYVLKTE"
     gene            complement(513..995)
                     /locus_tag="VBD11_001978"
     CDS             complement(513..995)
                     /locus_tag="VBD11_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6954881.1"
                     /translation="MDVSAIVRDIKTGRATLVCFPVEMSELKLALGLREEDDLEYIIA
                     DSDCQLLKEHDSIEMINQFVELVENVDSELVKAVHQVIGYTASDFVDYDFNFGDCCLL
                     SDVTTRRELGEYYFDELGVQGVGKEALEMYFDHEAYGRDIDLESQGGFSDYGYVEISG
                     "
     gene            complement(1048..1320)
                     /locus_tag="VBD11_001979"
     CDS             complement(1048..1320)
                     /locus_tag="VBD11_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954882.1"
                     /translation="MSGTRSEADKKLLVVTQELSELLVSHQYEQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
BASE COUNT          563 a          373 c          175 g          589 t
ORIGIN      
        1 ataaattatc gttaagcttc caattacctc ctcattctgt ttttaacacg taaaaacctt
       61 ttgaggtgtt taatactatt ccttcttcaa tataagcatt taaagcattt acccaagtta
      121 ctgaccgttg ccaaaaatca attaaagaat caaattcaac tttccttaag atatatttca
      181 ttactttatt ttcattattt gacaaaaata tataatattt tgtcactatt gaatgaactg
      241 ataaaccttt tttcaaaaga tattgaacaa tatagtcatc accatcataa agtccttcaa
      301 tacatctttt taaaagatag acttctgtaa gtgacataat cctatcaaca ttccaatcag
      361 aaaaaccaca aataaaaaag gcatcatcct taatgccttt ttctatcata cggtttctta
      421 cttgattttc cgtaaactgc atgatgtcta actcacataa aaattgactt ctaccttttt
      481 cacgagtcct tataattgca atcattatct acttaccctg atatttcgac atatccgtag
      541 tctgaaaaac caccttggct ttctaagtca atatctcgac cataagcttc atgatcaaaa
      601 tacatctcca gagcctcttt accaactcct tgtacgccta attcatcgaa ataatattca
      661 cctagttctc gtctagttgt gacatcagac agtaaacaac aatcaccaaa attaaaatca
      721 taatccacaa agtctgaggc agtataacct attacctgat gcactgcttt aacaagttca
      781 ctatcaacat tctctaccag ttcaacaaat tgattaatca tctcaataga atcatgttct
      841 tttagtaact gacaatccga atctgcaata atatactcta aatcatcttc ttcacgaagc
      901 cccaaagcta acttaagctc actcatttcc acaggaaaac aaactaacgt agctctacca
      961 gtttttatat ctctaacaat tgcactaaca tccatttttg acactcctta attaaaaaag
     1021 gagcaagcca ttgacttgct ctcaccctta atgaaattct tgtagtttat gaccaatcgc
     1081 tgacatagac ttatgagcct tattaatcat attattttgg tagtagtatg atttcaaatg
     1141 ttgttgaatc atgtcgacca tataacctgg taaagtcaat tcttcacgct ttttaagtaa
     1201 gctatttaac tctccagctt tttcccatga ctgttcatac tgatggctga ccaacagctc
     1261 tgaaagctcc tgagtaacca ctaacaactt tttatctgct tctgaacgtg ttcctgacat
     1321 tttattttcc tcttttttct ttagactcta acaataaatc aacctattac agttggacat
     1381 ctaattttca taattttcac ccacaaaaaa agaactccta taaagaagtt ctaattactc
     1441 atatataaat tacattacga ggattcaata cagaaagtta aaaattcatc taaattactc
     1501 ttatttgaag aaattaactt atctcttagc tcttttatcg tctccacatc ctgctcccct
     1561 cgttctacag gaaacagctt tttgtagtgt ctcttttctt tatcattctt agcataccaa
     1621 taaccaatat cattaaggac ctcctcgaaa ttgctatcat tccatatagg aacaataaaa
     1681 tcttttttcc aatcttttac
//
LOCUS       DATROY010000196         1622 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.197, whole genome shotgun sequence.
ACCESSION   DATROY010000196 DATROY010000000
VERSION     DATROY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.197"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..1604
                     /gene="uvrC"
                     /locus_tag="VBD11_001980"
     CDS             <1..1604
                     /gene="uvrC"
                     /locus_tag="VBD11_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6954883.1"
                     /translation="EDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYIKI
                     TNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCFYY
                     HLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERAAE
                     YRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNMFP
                     YYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKKQL
                     VNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGTSP
                     VAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLIVI
                     DGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEFFL
                     LHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKADI
                     EDIIMCGIPRTVAESLRDSLNDPPK"
BASE COUNT          558 a          241 c          318 g          505 t
ORIGIN      
        1 ttgaagactt tgaatacatt gtgacaacat ctaataccga agctctatta ttggaaatta
       61 atttaatcca agaaaatatg ccgaagtata atattcgatt gaaagatgat aagtcttacc
      121 catatatcaa aataacaaat gaacgttatc ctcgtcttat gattacacgt caagtaaaaa
      181 aatcagatgg cacttatttt ggaccttatc cggattctgg ggcagcaact gagattaagc
      241 ggttactaga taggctcttt ccttttaaga aatgtactaa tccagcaaat aaagtatgct
      301 tttattatca tttagggcaa tgcaatgcac atacagtttg tcaaactaat aaagcttatt
      361 gggatagtct gagagaagat gtaaaacagt ttcttaatgg taaagataat aaaattgtta
      421 acggattaac tgaaaaaatg aagagcgctg ctatgactat ggaatttgag cgtgcggctg
      481 aatatcgaga tttgatcgaa gcaattagtt tgttgcgtac taagcagcgt gttattcatc
      541 aagatatgaa ggatagagat gtttttggtt atttcgttga taagggttgg atgtgtgtac
      601 aggtcttttt tgttcgaaat ggaaaattga ttcagcgtga cgtgaatatg tttccttatt
      661 ataatgaacc agaggaagat tttctaactt acatagggca attttatcaa gatacaaaac
      721 attttctacc caaagaagtc tttattccac aggatattga tgctaagtcg gttgaaacaa
      781 ttgtaggctg taagattgtt aaaccacaac gtggtgaaaa gaaacagtta gtaaatctgg
      841 caataaaaaa tgctcgtgtc agtttacaac agaagtttga cttactggaa aaagatattc
      901 gtaaaactca tggggcaatt gaaaatttag gtaatcttct taatatccct aaaccagtac
      961 gcatagaagc atttgataat tccaatattc aagggacaag tccagtagct gctatggtgg
     1021 tatttgtgaa tggaaatcca agtaaaaaag actatcgaaa gtttaaaatt aagactgtta
     1081 tcggaccaga tgattatgct agtatgagag aagtcattca tcggcgttat agtagggttt
     1141 taaaagatgg cttgacacct ccggacttga ttgtaattga tggtggtcag gggcaagtaa
     1201 atatagcgcg tgatgttatt gagaaccaat taggtttagc catacctatt gcaggtttgc
     1261 aaaagaatga taaacaccaa acacatgaat tactttttgg agatccttta gaagtagtag
     1321 aattaccgcg taattcagaa gaatttttct tacttcaccg tattcaagat gaggtacatc
     1381 gttttgctat tacttttcat cgtcaattac gctccaaaaa ttccttttct tcaaaattag
     1441 atggtatcac tgggttggga ccaaaacgta aacaattgct aatgaaacat tttaaaagct
     1501 tacctaatat ccaaaaagct gatattgagg acattattat gtgtggtatt cctaggacag
     1561 ttgcagaaag tctccgagat tctcttaatg atcctcctaa ataaaaaact tagtagaaca
     1621 gt
//
LOCUS       DATROY010000197         1618 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.198, whole genome shotgun sequence.
ACCESSION   DATROY010000197 DATROY010000000
VERSION     DATROY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.198"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(52..636)
                     /locus_tag="VBD11_001981"
     CDS             complement(52..636)
                     /locus_tag="VBD11_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954884.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            725..1537
                     /locus_tag="VBD11_001982"
     CDS             725..1537
                     /locus_tag="VBD11_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6954885.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
BASE COUNT          613 a          295 c          225 g          485 t
ORIGIN      
        1 ttaaattaag gttaaataaa cttcaaacct aaaagactaa ccctgttgtg gttagtcttc
       61 cattaatatt ccccaaagta ataattgtac taaaaaaagg caagacaagg ctttaaaatc
      121 aaggttagcc atattagtct gtattccatt aaaaaatgct gacggattta aaatgattcc
      181 ttctaaacct aatgatcctg atttttctaa gcaaaaggca aaactactaa aaagcattac
      241 aggtataaca gttgctaatt gatacctttt actaatatca tatgcattta ccaatagctt
      301 caaacttagt aaagctgaag agataataaa aagaattgca agaaggtatg ccatatattg
      361 cataaagtga ttagactgcc aaatgtcttt tgttgaccac tctaagctca ctaaactaag
      421 aaaaatccct aaactctgtg ggtaaaaaaa taaaaggcct aaacttgcta aaagacgaat
      481 aaaaatatta cgactataat aagcaatgaa tcccatctct aacgacaaga ctgatgccaa
      541 tagaattacc atcaaatgat caatttttac atgataatac catatcccta aaatactgac
      601 tatggcaaaa agatgactcc aaattaactt tttcataatc caccaacttc catatttcca
      661 cagttattat atcatatttc tactgtctct ttttattgaa atctattttt gatataatgt
      721 cagcatgaca aagaaaatta tcgccgtaga tttagacggt actctattac ataataataa
      781 cactatttct gactacaccg ctgatacact tcgaaaagtt caagctcaag gacacaaagt
      841 aattattaca accggacgcc cataccgtat ggcactcgct cattatttac gcctagattt
      901 aaaaacacca atgataaatt ttaacggtgc tttaactcat atccccgaaa aaaagtgggc
      961 gtttgagcat agtgcaacga ttgataaaaa attactcctt gaaaccttaa atttatcaga
     1021 tgctatccaa gccgatttta ttgctagcga gtaccgtaaa aatttttaca ttactatgga
     1081 taatcgtgat aaaattaacc cacaattatt cggtgtcaat gaaattactg ataaaatggc
     1141 attagatgtt actaaaatca cacgcaatcc aaatgctcta ttgatgcaaa cacgtcataa
     1201 agataagtat gagttagcta aagagttacg acaacatttt aatcacgaac tagaagtaga
     1261 ttcttggggt ggtccattga atatactcga attttcacct aaaggtgtta acaaagctta
     1321 tgctctcaaa catttattaa aatcactcaa tctttcacaa gaaaatttga ttgcctttgg
     1381 agatgaacac aatgacaccg agatgctggc ctttgcacat acaggatatg ctatgaaaaa
     1441 tgctagtcca actctacttc catatgccga ccaacaaatc caatggacaa acgaagaaga
     1501 tggtgttgct aaaactctag aaaaattatt attgtagtat tttcaaaaac gatctctatt
     1561 gaaacagaat tctaatagaa atcgtttttt aggttttcta ctatattatt aacaaaaa
//
LOCUS       DATROY010000198         1617 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.199, whole genome shotgun sequence.
ACCESSION   DATROY010000198 DATROY010000000
VERSION     DATROY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.199"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..721
                     /locus_tag="VBD11_001983"
     CDS             <1..721
                     /locus_tag="VBD11_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6954886.1"
                     /translation="SRSDVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQYDKLTH
                     AGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTSKHGNFD
                     FPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVTKLNSIT
                     SQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAMPGAQLYM
                     MSIDQSSLSNAKSKIKNTMEE"
     gene            727..1458
                     /locus_tag="VBD11_001984"
     CDS             727..1458
                     /locus_tag="VBD11_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO6954887.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
     gene            1467..>1617
                     /locus_tag="VBD11_001985"
     CDS             1467..>1617
                     /locus_tag="VBD11_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO6954888.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQY"
BASE COUNT          590 a          240 c          277 g          510 t
ORIGIN      
        1 atcaagatct gatgtaaata ttattatgac ggttaatacc aatacccaca aagtattgtt
       61 aacgacaaca ccacgagatg cctatgtaaa aattccagat ggtgggggca atcaatatga
      121 taaattaacc catgcaggtt tgtatggtgt tgagacatca atgaaaacac ttgaaaacct
      181 ttacgacatc aaccttgatt attatgctag aattaatttt tcatcatttt taaaattaat
      241 agacctcttg ggaggagtga cagtttataa cgatcaagct tttacaagta aacatggtaa
      301 ttttgacttt cctgttggtc aagtaacatt gaattctgag caggctttgg gctttgttag
      361 agaacgttat tctctacaag gaggcgataa cgatagaggt agaaatcaag aaaaagtgat
      421 tgcagctatt ataaataagt tagcttctag tcagtcagta acaaaattaa atagcattac
      481 ctcacagctc caaacgtccg ttcaaactaa tatgactatt gataatatta atgatttgat
      541 taacaatcaa ttgtcaactg gacaacgctt cactgtcgag tcacaagcat taactggtca
      601 tggttcaacg ggtgaactcc cttcatatgc aatgccagga gctcaacttt atatgatgtc
      661 aattgatcaa tctagcttat ctaatgcaaa atcaaaaatt aagaacacaa tggaggaata
      721 acaagaatga ttgatattca ttctcatatc gtttttgatg tagatgatgg gcccaaaacg
      781 cttgaagaga gtttatcttt aatagaagaa agttatcgac aaggtgttag aataattgtt
      841 tcgacatcac atagaagaaa aggtatgttt gaaacacctg aagatattat cttcaaaaac
      901 ttttctatag ttaaacacga agcggaaaaa agatttgaac atcttcaaat cttatatggt
      961 ggagaattat actatacaag tgatatgtta gaaaaactga agttaaagca aattccaact
     1021 ttaaacaata cgaaatttgc tttaattgaa ttttctatgc aaacttcttg gaaagatatt
     1081 catacagctt tgtcaaatgt tttaatgctt ggtattacac cagtcgttgc gcatatagag
     1141 aggtataacg ctttagagaa tcaaaaagaa cgggtgaagg aaattattaa tatggggtgt
     1201 tacacacaaa taaatagttt ccatattttg aaacaaaaac tttttaatga taagcataaa
     1261 cgctttaaga aaagagcccg ttatttttta gaggaaaatt tagtgcattt tgtagcgagt
     1321 gatatgcata accttgatgt tagaccgcca tttttagcag aagcttataa gattatctgt
     1381 agagatttcg gtaaagaacg tgctaaccaa ctttttattg aaaatgctca atctatatta
     1441 aaaaaccatt acatttagga gatttcatga ataaaatagc taatacagaa gttgaaataa
     1501 atatattcaa ccttttaaaa aaattgtgga aaaagaaatt tttaatcaca tttgttgcca
     1561 ttgcgtttgc aacagctgga cttttctata gtctttttat tgttacgcca caatata
//
LOCUS       DATROY010000199         1584 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.200, whole genome shotgun sequence.
ACCESSION   DATROY010000199 DATROY010000000
VERSION     DATROY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.200"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..1488)
                     /gene="gtfA"
                     /locus_tag="VBD11_001986"
     CDS             complement(<1..1488)
                     /gene="gtfA"
                     /locus_tag="VBD11_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6954889.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDICKKHEYSYRIASRFLNDKIIENWSFF"
BASE COUNT          526 a          302 c          196 g          560 t
ORIGIN      
        1 aaaaaaactc caattttcta taattttatc atttaaaaat ctactagcta ttctataaga
       61 atactcgtgt tttttacaaa tatctttaga attaaataaa gcaacaatat actgagcaaa
      121 agcagttgat atttgttgag gattatctgg tgtttccctt ggaataaggt agccattcaa
      181 attattttca ataaaggtct gattaccata cggaacatct aaaccaatta tcggaagccc
      241 cgatccgatt gcctccatca aggttaaacc aaaaccttca ctggtagaac ccgataaata
      301 aacttgataa tctttataaa cagaagatag gtttttatgc cccattaatc gtatataatt
      361 atttgcctta tttttagcaa ttatttcttg taacttttgc cgttcgccac cttcaccata
      421 aatatcaaag gttaattcag gaattacttt attagcttct accacagcat taattaacca
      481 atcaatatgt ttttccgaag ctagacgtga gcaagttaac aacgaaaaag cttttctttc
      541 atgccactct tttctctgta attttgataa acttcctaca ggaattgtat aaacgaccgg
      601 ttcaatattt aaatactttt taaattgatc aatcaacgtc ttagtttgcg tttctgttga
      661 ggtgataaaa gcatctatgc tatcagcatt tgaaaattga tagtcataat aattattcca
      721 taaaatcgta gtttcatcag ttgcgctaac attataatgc tctgcatgaa caacaacagc
      781 tactttagca tttcctttat tccttagaac agcttgccca atgcctgtag agcggtctaa
      841 caagattaga tcactattcg ttaattgtaa tttttctaac atgtatccaa ctaattcttc
      901 tttagaatat agtatagttt ctttgaattt aaaaatacta ttctcgccat ctacattttc
      961 ttcatatgca acacttccat cttcattata aaatgtacgc tgatacaggc atggacctat
     1021 atcttcggga gcataatatt ctgaaaacat tttagtatat gagtagtagt cttttctaac
     1081 aagctttccc cccataagat actctaccct ttggacgatg ttactagatt catcgttaag
     1141 cataactgta actacaatac tacttccttt aaaaaaaaat gatattattt taccgttttt
     1201 ctcagtccta tcaatcggta gtgaaaaact ttctttcaat tgttgcagtg aataagaagt
     1261 tgcagcaatt gttaaatcag taaaaaaagt ataaagccat ataatctcat tgtcttcaaa
     1321 gccgatattt cgagtaaggt gctctatatt ttcattctga aacatatcag taaaaataaa
     1381 tttagcttca acacctaact ttctaaaaac ttctgaacga tatgcttgag catactctac
     1441 accgctactt gcccaaccta tccctctatt caaattataa actaccatta actccgtttt
     1501 cctttataca taatatattg agtaacttcc atcacttgct tttgaaattg tacttaacat
     1561 aatattctta acgatagaat atct
//
LOCUS       DATROY010000200         1560 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.201, whole genome shotgun sequence.
ACCESSION   DATROY010000200 DATROY010000000
VERSION     DATROY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.201"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(149..1501)
                     /locus_tag="VBD11_001987"
     CDS             complement(149..1501)
                     /locus_tag="VBD11_001987"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6954890.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFIVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK
                     "
BASE COUNT          476 a          265 c          264 g          555 t
ORIGIN      
        1 catcttaatt gtccgaattt gtcagtgata ccaagtagtt acgattaaaa ttgaaaaaac
       61 tatcctatgt tctaaataaa atcgtcctaa gtaaacttac ttaggacgat ttgttatcta
      121 tctaatagtg taagtagtgt aataggtact atttttggaa gaatgtttca tatagtgctt
      181 taatagctct tttttcatcc ttactgtcta taacgaacat aatggagact tcgcttgaac
      241 cttgtgatat catggtgata ttgatttttt ctcttgataa ggcttgtgtc gctgttgcag
      301 tgactccaat ctgacttttc atattttcac ctacaattac gattgtagat aaattgtgtt
      361 ggatgtcaac gtaatctact tctagtttac gagttaggta atttaagatt tcttgttctt
      421 tgattggtgt caattctttt tcacgtaaga caatggatag atcatctatg ccagttggca
      481 tatgttcaaa actaatattt aaatcctcta aaatttgtag tacttttcgg ccgaaaccta
      541 cttctctatt cattaagtat ttagatacgt ttatgctagc aaaacggcta tcagaagcga
      601 tcccagttac tgctatgtta ctacgagtat gctttaaaac tatttttgta ccaggctgtt
      661 ggggattatt tgtattttta ataacaagag ggattctgcc acgataggca ggaagtaaag
      721 cttcatcatg taaaaccgaa aaacccgcat aggctaattc acgcatttct ttataagtaa
      781 gctcagggat agcgtgaggg ttcttaacta cacctggatg cgctgcaaat ataccatcaa
      841 catctgtgaa gttctcataa aggtctgctt ttatgcctgc tgcaattagg gatccagtaa
      901 tgtcagatcc ccctcttgaa aaggtacaaa tttggttatc ttctgtgact ccaaaaaatc
      961 cagggataac aagaacctca ttgtatagac ataagttctc aatcttatca taacttcctg
     1021 gaataattcg tgcattacat ggttctttag ttacaataat tcctgcttcg tttggatgaa
     1081 catatcttgc aggaatgtca ttttgattaa aaaacgttgc aactaacttt gcgttgttat
     1141 cttcaccggc tgctaaaaaa caatcatata aaaactgatt attttctatg ggaagtgaag
     1201 ctagctgctc tatagagcga gtaatttctt ctgcaatagt gcttcctaaa gacaaatcac
     1261 taataatttc ttgataacga ttgataatcc aagtttggtc tttaacaata tctttaccgt
     1321 taatataatt ttgataatat tgtatcaaag catctgtcat ttttaagtct tcttcgtagc
     1381 gtttccctgg agctgaaacg acaataaacc gtctagtgta gtctgatttg ataatattta
     1441 gtactttgta taattgttgg gaagatgcta aagagcttcc tccaaatttg actactttca
     1501 taggttacct caagaatgaa ttttagataa attataacaa aagtcagaaa atttgtcatt
//
LOCUS       DATROY010000201         1537 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.202, whole genome shotgun sequence.
ACCESSION   DATROY010000201 DATROY010000000
VERSION     DATROY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.202"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..767)
                     /locus_tag="VBD11_001988"
     CDS             complement(<1..767)
                     /locus_tag="VBD11_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954891.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIV"
     gene            complement(770..1486)
                     /locus_tag="VBD11_001989"
     CDS             complement(770..1486)
                     /locus_tag="VBD11_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954892.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          490 a          257 c          234 g          556 t
ORIGIN      
        1 acaatgatga cactaaaaat actacctaca acttgaatat ctagtagcca aatagttgat
       61 aaaacgataa ctatcgtttg aataattgct agaatgccgt atgataacat atatccaaat
      121 acaatatcac tacgtttaac aggagttgca aggagtcgat caagtgtacc agaagtacgc
      181 tcttttaata aagccattcc agaaattaaa aaaacaaaga agaagaccat aaatcccatt
      241 agtattggta tcatttttgc aaaaaagcct gtatttttat ttccgtaatt atatttttct
      301 ttgatatatt tggagcgagt ttgtaaggat tgtgctagtt tcggattctt attagctaaa
      361 attttaactt gcgaaatcag ttccttactg ttcattgtat taacagcggt tttaaaagct
      421 tgtcttgtta aagtcgtctt tgaagaatct gtattcgcat agaagacagt ataagattta
      481 ttgtcctccg aaataagagc atcaatttta tttgatttga gtgctttttt agcagatgag
      541 ttaaatttaa atgatctcac ttgaatatgc ttaatattat ctaaatttga aacgaccttc
      601 gtgttaacgt taatagttcc aatcttaact tttgtattac tattcgcaga aaacataaca
      661 ttcatcaaaa acataattaa aataggtgct aaaaacatca tagcaagtgt tcttttatca
      721 cgaaacagtt cttttataac cttttcagtt attgcaataa ttctcatgat tattctcctt
      781 cagcttttaa gaaaacttcc tcaatagtac tcacattaaa ttgttttttt aaatgtaatg
      841 gagtatcaaa ggcaataatg tttccacgta atagtagtgc aaccttactt gttaattctg
      901 cttcatccat aacgtgggtt gtaataaaga tagaacgtcc ttcatcctta atattaatta
      961 gctcttgcca gatttttctc ctcaaggatg gatcaattcc aacggtaggt tcatctagga
     1021 ttaaaactgt ggggtttcca agtagggcga tggctagaga aagccgtctt ttcatacctc
     1081 ctgagtaacc tgagacaaat ttatcaagtt ggttttctag atctactact ttagaaatat
     1141 gagttatctg ctgttttaat tcagtttttt gaataccttt catttttcca aagaataata
     1201 aattttctaa gccagttaaa gactcgtgta aggcatcaga ttgagccata tagccaattt
     1261 gatttaaaat attacgatct ggcatttgag tatcaagaac aagagctgtt cccttatctg
     1321 ctttttccat gccaagcata gttttaatca aggtagattt ccctgctcca gagggtccta
     1381 ttaatccaat tatttctcct ttaaacacct ccaaattaat attatttaaa acagtttctg
     1441 aggcgtatgc tttttgtagt ttttttaaat cgatgacttt tttcatgata actttttgct
     1501 cctctctcag tgagtgagtg tttactcacc aatttga
//
LOCUS       DATROY010000202         1517 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.203, whole genome shotgun sequence.
ACCESSION   DATROY010000202 DATROY010000000
VERSION     DATROY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.203"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..97
                     /locus_tag="VBD11_001990"
     CDS             <1..97
                     /locus_tag="VBD11_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion accessory protein EsaB/YukD"
                     /protein_id="HEO6954893.1"
                     /translation="VINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            97..1371
                     /gene="essB"
                     /locus_tag="VBD11_001991"
     CDS             97..1371
                     /gene="essB"
                     /locus_tag="VBD11_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6954894.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYYAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
BASE COUNT          533 a          239 c          265 g          480 t
ORIGIN      
        1 agttataaat aaaggtattt tattgggaga gaacgatata ttagcgcacc atccagtaac
       61 aactggtgat cgtataagga tagaggagtt ttattaatgg aaacagattc gtttgtattt
      121 gaagaacaag actttcaatt tgaaaaagga ccagattttt ggcagttatc tcttaagcgt
      181 tcaaaagtag gaactcaaaa tctgcaacaa ctgcaattgt tagaaattca ccattctttg
      241 ttgatgccta tgacaactgc aattaatgca gataccattc aatttcaatt ccagacagaa
      301 gcgcacgctc tatcttttga aagctttaaa aaagaaactt tatctgaaaa attacgatta
      361 gccttgaatg tcttggattt agataaggct ttgtctttgc cagtaaattt cattttgcat
      421 ccctctaatc tatttttaac aaaaaatgca actgctaaaa tagcttatcg tagtcttcct
      481 gggattatga cacctgagaa gtttggtcca gaggagtttt tatatcaatt caaatgtttt
      541 gtctttgcat tactgacgca acatgactat atagaattgt ataatggtgc tatttctgtt
      601 attgaagtat cagatttcct aaaaagcatt tattatgcag aaactattca ggctgttaga
      661 gacatcatta ctactgatta tgagcagcaa gtagaagtag aaactcatac gttagcaaaa
      721 gtatcaaagg caaaatataa gctttataaa tacataagtg tctggctagg agctttatct
      781 acaatactct tgattccgct agtatatctg gtttttattc acaatccatt caaagaaaaa
      841 atgttggcag cggatacttc atttattaaa gttgattata atcaagtgat taatcgacta
      901 gaacatgtaa aagtaagtaa gttaccttat acacagaagt acgagttggc ctattcctat
      961 attaatggaa tgtcattttc tgaagaacag cgtgaagtta ttttaaacaa tgttacgctc
     1021 aagactgatg agctctatct tgattattgg attaatattg gccgtggttt agatgatgat
     1081 gctattgatg ctgccaaacg tttagacgat tctgaccttg ttatttatgc tattgttcag
     1141 aaaatggatc aggttagaaa agacaatagt ttatctggta aagatcgtga acaaaaactt
     1201 tctgagctac aaacagacta tgataaatat tggaaagacc gtaagacagc cttaacagat
     1261 gaggaatcca aatcgaaaaa tagcaataat cattcgacaa attccaacaa agagtcatct
     1321 gaatcatcaa gtactacagc aagtacatct tctaaaacta aaagtaggta ggtggatatg
     1381 ttagttattt gttattatca atcactccgc tacgaattta atcttgaaga ggagaaatcc
     1441 tttctaatat catcaaacgg caagtcgcca ataccagtat cagatttaga aaatgacatc
     1501 acgctaaaaa atatgca
//
LOCUS       DATROY010000203         1422 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.204, whole genome shotgun sequence.
ACCESSION   DATROY010000203 DATROY010000000
VERSION     DATROY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.204"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            251..660
                     /locus_tag="VBD11_001992"
                     /pseudo
     CDS             251..660
                     /locus_tag="VBD11_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            735..1415
                     /locus_tag="VBD11_001993"
     CDS             735..1415
                     /locus_tag="VBD11_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6954895.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
BASE COUNT          555 a          145 c          263 g          459 t
ORIGIN      
        1 ttagaataat tttatagact tacaaagaaa tttaaagctt ataatattgt atttttggag
       61 attattgaag tggtttatag ataattaagg aaggaatatt gattagaaat gaataatttg
      121 gatacactat attgtaattt agcaatgtgc aatcatagta ctaatggaaa ggtagcgact
      181 tcagcttgtt gatttatcta atgtaaatga gtttttagaa ttttcatctg attttgaaac
      241 tgtatttcat atgcaacgat ataaagccaa taccacagaa gaagcacatt gattttggcg
      301 aatgtctgta tgaaatcatt attaggaatc tatgcgatga ttgaaacaga atcaaataaa
      361 atgattggta ttatagaatt agaaattaga gacgagtttc cagcagaatt cggttatata
      421 ttaaataaaa attatagtgg aaaaggttat atgataaaag cttgttcaaa attgatgtca
      481 attggatttg aacaactaga tttggaaaga atttatgctc gatttgaact aataataaaa
      541 aatctgaaaa tgttatggaa tgcctaagaa tgcaaaaaga aggtgaacgt cgtcatattg
      601 taaaaaaatc caaaaggtga gtggaaaacc agagcatatt attctatttt aaaggaataa
      661 tattttaaat agacgacaat aaaaatttgt gaaagcagaa aaattaaatg agatacaata
      721 ggtttaatca actagtgggt gatgtcgttg aaaattttac ggaaggtaaa aatccgaaga
      781 ttgatacact taatgggaaa acagttagaa tagaaaaaat aaatccagat cattttgaag
      841 acttatttca agtttatgga gaattgtcta ctgaagatag tttaacttat atgtctttta
      901 gcaaatttaa tagtaagaat gaatttgatg tctttttcca aactctatta aaatcagaag
      961 atccttatta tttagctatt gttgataata atactggtaa agcattaggg actttttcat
     1021 taatgcgtat tgatacaaaa aatagagttg ttgaaatggg atgggtggtt tattcatcaa
     1081 aactaaaaca aacaaggata gcaacagaag ctcaatattt ggttatgaaa tatgtttttg
     1141 aagaactctg ttaccgccgt tatgagtgga aatgtgatag tttgaatgca ccatctaata
     1201 attctgcaaa acgacttggt tttacttttg aaggaacttt tagacaagct gttgtatata
     1261 agggtcgtaa cagagataca aattggtatt ctattttaga taaagaatgg ccagagaaaa
     1321 aaacacggtt tgaaagatgg ctagatgatt caaactttga tgtaaatgag tatcaaatca
     1381 gatcactatc aagcattgag cagaagacaa aatagagtca tt
//
LOCUS       DATROY010000204         1416 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.205, whole genome shotgun sequence.
ACCESSION   DATROY010000204 DATROY010000000
VERSION     DATROY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.205"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            127..1410
                     /locus_tag="VBD11_001994"
     CDS             127..1410
                     /locus_tag="VBD11_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6954896.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
BASE COUNT          510 a          255 c          232 g          419 t
ORIGIN      
        1 ctatcattca ctgctttatt ttaggataac taaacaattt attgtttaaa atgaaaggat
       61 ttatgaatgt ctaggaaaac atttaaacgt atactatcga ttggagtttg cacgctcgta
      121 ctatcgatga gtctttatta cactgaaaaa gcccacgcta ttgctggtcc tagtgaccgc
      181 caatacgtag aaaacccaaa tcctcacatt attgtaaatg ttacaggtac tgatcaaaac
      241 ggaaatagca ttttaccgca ttatatcgaa gtcaatgtaa agatgggaca aactttaagt
      301 aaagaagaaa ttctagatta tattgctcga aatttaaact ctagtgttgg aggagaaagt
      361 aaaaacgttc aatacagcaa catcgagttt aaggaaagtg cttatctgaa gcgtcaatta
      421 gatgatggca agacagaaga aatagcaatt gataacgacg gtgttactgt acctaaagac
      481 ggtccaaaca aattttggat tgacgttcca gtaacttgta ctgttactcc tatcgtaaca
      541 gaaacacatg aagttcgatg ggggactcca gtcgctatat cacaccgtat ttactttgtt
      601 gaagaatctt ctggaaaagt tttagatgaa tacacaaatc tacacactgc tgattcggaa
      661 cttaacggtt atcgtgttgg agattatatc acagactatg cactttctaa gtctgcttac
      721 gaagcttttt taaactctcg tttagataaa gaaggttaca aacttcaaca tcgtattagc
      781 acgaatgtac gacaaaacct tcaaattaat aaattgattt tcaattatga ctttaatgag
      841 gaaaatattt actaccaaat cgggaatatc cgtccactat taagtcgctc atcagctgaa
      901 gtagaatctg acatcattac agaacgctac tatgtttcta aaaatgctaa aagtttagca
      961 cgtacagaat caaccatttc gattaaaatg gttgatgcca aaactgaaca accgctattt
     1021 aaccacacat taactggtta tcaattggta actgtctccc atgtctataa cagactcttt
     1081 gaagaaaatc ttatcccaac tacaaaatca ggagaaagat attttattca aaatatgaaa
     1141 aaaacagctg aacaagaata tactgtttat ctttcagaaa caccttattc taaagagaac
     1201 gctcccgtaa tttcttatga tgcaagacct gttgattggg attatcactc aggcgcttca
     1261 ggatcacttg aaaatcagcc taacatctat actgaagaag attcaactga atttttgggt
     1321 aataaaccac aagcagcttg ttatccaaac aaacaatttg cttgcgaaaa tactgactct
     1381 aaatacaact atagctattt agaaaaatag ttataa
//
LOCUS       DATROY010000205         1367 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.206, whole genome shotgun sequence.
ACCESSION   DATROY010000205 DATROY010000000
VERSION     DATROY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.206"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(133..444)
                     /locus_tag="VBD11_001995"
     CDS             complement(133..444)
                     /locus_tag="VBD11_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6954897.1"
                     /translation="MSNDSIFSGIYSPNSSSQVPTTPQYQAPSPSPQAPTTPQYQAPS
                     PSPQAPTTPQYQAPSPSPQAPTTPQHQAPSPSSQIPSTPNTPSNNFGSGDNGIDFGEG
                     L"
     gene            416..1321
                     /locus_tag="VBD11_001996"
     CDS             416..1321
                     /locus_tag="VBD11_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6954898.1"
                     /translation="MPENIESLDIQIWVNKLAKQSVKSVASGVKKSKKGHSKDFGAFV
                     HKLDDIFDFGVINFGLQSNPVKGIKIPPKPKSLKKRIRVLHDNELTIWLEYLEILENN
                     RANRRFKIICNTLLASALRINELLALTIDDLDFMTNEIIVSKTLVWKAGNKNKGIKGE
                     MICKQSPKSDSGNRRVLVPKEILNGLKDFHQEMNNYFEDHRLPTTKLIFPTIYGNYMC
                     DRNERLTLKKRLSSLNLPDYGFHIFRHTHASMMLNAGANWKELQVRMGHKSISTTMDT
                     YAELAPTKKQEAVEIFLNKISELTH"
BASE COUNT          476 a          190 c          252 g          449 t
ORIGIN      
        1 acttcccata agaattattt atatttgata atatatcata ttttataata ttatataact
       61 attaattaat caaaaagaac cttcaaaggt tctttttatt tttcttcaaa aattgatata
      121 aattctattt ttttataatc cctccccaaa atcaattcca ttgtctccac ttccaaagtt
      181 attagaaggc gtattaggtg tagacggtat ttgtgagctt ggagaaggtg cctgatgttg
      241 aggtgttgtc ggtgcttgtg gacttggtga aggtgcctga tattgaggtg ttgtcggtgc
      301 ttgtggactt ggtgaaggtg cctgatattg aggtgttgtc ggtgcttgtg gacttggtga
      361 aggtgcctga tattgaggtg ttgtcggtac ttgagagcta gaattcggag aatatatgcc
      421 tgaaaatatc gagtcattag acatccaaat atgggttaat aaattagcta aacagtctgt
      481 taaatcagtt gcatcaggtg ttaaaaagtc aaaaaaagga cattccaagg attttggagc
      541 tttcgtccat aaactcgatg atattttcga ttttggagta ataaattttg gattgcaaag
      601 caaccctgta aagggaatta aaattccgcc aaaaccaaaa tcattaaaaa aaagaattag
      661 ggtattacat gacaatgaac taactatttg gttagaatat ttagaaattt tagaaaataa
      721 tcgggctaat agaagattta aaataatatg taatactctt ctagcatcag cacttagaat
      781 taacgagcta ttagctttaa caattgatga tttagatttt atgacaaatg aaattatagt
      841 cagcaaaact ttagtgtgga aagctggtaa taagaacaaa ggtattaagg gtgagatgat
      901 ttgtaaacaa agtccaaagt cagactcggg aaatcgtcga gttctagttc caaaggaaat
      961 tttgaatgga ttaaaagact ttcatcaaga aatgaataat tattttgaag accataggtt
     1021 accaactaca aaattaattt ttccaacaat ctatggaaat tatatgtgtg atagaaatga
     1081 acgtcttaca ttaaagaaaa gattgtcatc acttaattta cctgattatg gctttcatat
     1141 ttttcgtcat actcatgctt ccatgatgct aaatgcaggc gcaaactgga aagagcttca
     1201 agttagaatg gggcataaat ccatttctac cactatggat acgtacgcag aattagcacc
     1261 tacaaaaaaa caagaagccg tagaaatttt tctgaataaa atttctgaat taacacatta
     1321 acagctctac atttttctct acattttctt ttaaatttag ataaaaa
//
LOCUS       DATROY010000206         1332 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.207, whole genome shotgun sequence.
ACCESSION   DATROY010000206 DATROY010000000
VERSION     DATROY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.207"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            64..>1332
                     /locus_tag="VBD11_001997"
     CDS             64..>1332
                     /locus_tag="VBD11_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954899.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFIIEMVFISIPALLGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSGLDINVLPKFI"
BASE COUNT          539 a          166 c          233 g          394 t
ORIGIN      
        1 cattttagaa ctaagtggta aaaagttgaa aaaagtaaat aaaatgaatt tggaggttga
       61 ataatgataa aaaatgcatt tgcttatgta actagaaaaa gcttaaaatc attaattatt
      121 atattagtta ttctatcaat ggcaacttta agcatcatta gtttatctat taaagatgcc
      181 acagatagag cttctaagga aacatttgct aatataacaa acagtttttc tatggaaata
      241 aatagacaag taaatccagg aacacctagg ggtggaggaa atgtaaaagg tgaagatatt
      301 aaaaagatat ctcaaacaaa cagtatagac tcctatgtaa aaagaataaa cagcgtcgca
      361 gacctagttg atcatgatat aattgaaact caagatacac ttgcaaatca atcacctgaa
      421 agagcaaaga attttaagag aacagttatg ttaacagggg ttaatgactc agcaaaagaa
      481 acaaaatttg tatcagaagc atataagtta gtagaaggaa agcatttaga aaataaagat
      541 aaaaataaaa ttttaatgca taaagatttg gcaaaaaaaa acaatctcaa agttggagat
      601 aaaataaaaa taaaatctaa tttgtttgat gctgacaatg aaaaagttgc aaatgaaact
      661 gtagaagtag aaattaaagg tttatttgat ggtcataata gcggtggggt aagtgcagca
      721 caagaactat acgaaaatac actaatcact gacgttcatt cagctgctaa agtttatggt
      781 aatacagaag atacagccgt ataccaagat gcaacattct ttgtaaaagg tgataagaat
      841 cttgatagtg taataaaaga tcttgggaaa ctagatataa attggagaga atataatttg
      901 attaaaagtt catcaaatta tcctgcgctg caacaatcta tatccggtat ctattctatt
      961 tcaaacaaat tatttgttgg ctcattaata tttgcaggag ttgtagtttc attattactt
     1021 ttcttatgga tgaatgccag aaagaaagaa atagcagtat tactatcatt aggtatatct
     1081 aaattagaaa tttttggtca atttataatc gagatggtat ttatatccat tcccgcactt
     1141 cttggatctt atttcttagc tcaatataca gctgataagc tagggaataa tatattgaat
     1201 aaagttactg gcgatatagc taaacaaata gctagacaat ctgcatctag tcaattaggc
     1261 gggggggcag aagctgaagg attcaacaag actctttcag gtttagatat aaatgtatta
     1321 cctaaattca ta
//
LOCUS       DATROY010000207         1301 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.208, whole genome shotgun sequence.
ACCESSION   DATROY010000207 DATROY010000000
VERSION     DATROY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.208"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBD11_001998"
     CDS             212..1288
                     /locus_tag="VBD11_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954900.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
BASE COUNT          465 a          181 c          219 g          436 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaata tgaatacaga a
//
LOCUS       DATROY010000208         1297 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.209, whole genome shotgun sequence.
ACCESSION   DATROY010000208 DATROY010000000
VERSION     DATROY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.209"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..104
                     /locus_tag="VBD11_001999"
     CDS             <1..104
                     /locus_tag="VBD11_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6954901.1"
                     /translation="SYFIKYELINNSKEELFNRKVIEKIIRTSIENY"
     gene            114..1100
                     /gene="dhaQ"
                     /locus_tag="VBD11_002000"
     CDS             114..1100
                     /gene="dhaQ"
                     /locus_tag="VBD11_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6954902.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
BASE COUNT          454 a          183 c          264 g          396 t
ORIGIN      
        1 tctcttattt tataaaatat gagttaataa ataactcaaa agaagaatta tttaatagga
       61 aagtcattga aaaaattatc agaacatcaa tagaaaatta ttaggacaag gatatgagaa
      121 aaacagaaca tcataaaatg gaatatgttt ttgaaacaat aaaagcaatc actaaaacta
      181 atcctaaagt agagcaaata aaagatttgc ccgctattca ttatttaggg caatccgagc
      241 aagtaattcc gattttatca ggaggaggca gcggacatga gccggctcac tttggatatg
      301 ttggtgaggg aatgctatcg gcagcgatta gtggtcctat ttttgtacca ccatgtgcta
      361 gtgatattct agagactatt agatttatca atcgaggcaa aggtgtcttt gttattatca
      421 aaaattttga agctgatttg gaagaatttt cccaagcgat tgaacaggct cgtcaagaag
      481 gcattccaat caaatacatc gtttctcatg atgacatctc tgttgaaaca tcaaatttta
      541 aaattcgtca tcgaggcgtt gctggtactg tgttgctaca taaaattatt ggacaagctg
      601 ctttagaagg agcaagctta gatgaattag aacagctagg actatctttg acaacttcaa
      661 tggctacttt aggtgtagca agtaagtcag ccactatttt aggtcagcat caacctgttt
      721 ttgatttaga agaaggttac atctcatttg gaatcggtat tcatggtgag ccaggatacc
      781 ggacaatgcc tttcgtgtcc atggaacatt tggcaaacga actagttaac aaattgaaaa
      841 tgaaattacg ttggcaagat ggagaagcat tcattctatt gataaataat cttggaggaa
      901 gttctaaaat ggaggaatta ctttttacaa atgctgtcat ggaatttctg gctttagacg
      961 atttacaatt accttttatt aaaacaggcc acctaattac aagtttagat atggctggtt
     1021 tatcagttac cttgtgtcgt gtaaaggata gtagatggat tgattactta aaacataaga
     1081 cagatgctag agcttggtga tagaagtaag tggtaagagt ttttagaata gaaaatactt
     1141 taaggagaac agagaaatct gttctctttt tgttcgctta aagatcatag aaaggtgaag
     1201 agaacatgga aggtattgaa ataatattag atatagaaac gttaggaatt tttcaagtca
     1261 tagatcatgg tatctctcca gatgatagag aaacata
//
LOCUS       DATROY010000209         1253 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.210, whole genome shotgun sequence.
ACCESSION   DATROY010000209 DATROY010000000
VERSION     DATROY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.210"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..318)
                     /locus_tag="VBD11_002001"
     CDS             complement(<1..318)
                     /locus_tag="VBD11_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6954903.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNP"
     gene            540..1136
                     /locus_tag="VBD11_002002"
     CDS             540..1136
                     /locus_tag="VBD11_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6954904.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT          470 a          221 c          176 g          386 t
ORIGIN      
        1 aggattatct aattcatata aaatattaat ggctaaacca tcctcataga aaacatgtgt
       61 ccataaaata cgattctcta aaaaaagctg tgaaatctct aaaggctttg cagcaaaaac
      121 aaggttacgt tcatttttta gaaggtgatt aatatctgcc atcaattctg gatagtctga
      181 tactggaaat gtttcgtagg ctactttgaa tttattccag aaatagcgag cttcatttgc
      241 acgcaaacca gccaaggctt ctgccactgg agaagtctcc aaacctttag ttgaaacggt
      301 tttaaagtcg ataatcatgt tctttccttt ttgattaaaa tcttcgataa tttagtatac
      361 tcttttttga agaaaaagta cattctaatc acttataaaa agtagtacat ttagaacact
      421 accacctcag taatttcttg acctttctct acttaatact tataattaat ctagacttaa
      481 tgtcaaagca tcaatcaagg tgtaaacctg attcacctaa aaagctgatg gagctaaaca
      541 tgaccaaaaa gatattctta tttatctgcg ctactctttt agtaggaata gccttatata
      601 caccaacaac ttctattttt aatcataaca acgtttatgc tgccaccaag aaagcaaaaa
      661 ttaaatttaa taaaactcaa aaaaaaattg tcaaaaaagc aagagaatat gctaagtctg
      721 gccatatgtc taaagatagt atcattgaaa aattaaaaaa ggattctaag aaatatcgtc
      781 aagaagacat caactttgtg attaataacc tcaaggttga ttacaaaaaa aatgccttga
      841 tttctgccaa aatttattca aagaccatga atttatctaa acaatctatc tttgaacagc
      901 tctattcgga gtctccagat aaggcaaccc actctgataa atttacaaaa gaagaatccc
      961 aatatgctat agaccatctc aaagtagatt tcaaagaaaa tgctcttgag actgctaaaa
     1021 gttatcaaag tagttcttct ttatccaaag aagaaatcta taaacaattg acatctacgc
     1081 tgggcgataa attcacaaat gatgaagccc aatatgctgt agatcatttg aaatgatgac
     1141 ataccaatag tctatcctta tgtaatagaa ttgatagctt atgctaatat tccagctgta
     1201 aaatagcaag aaccctccta agttcattaa gacttaggag ggtttttatc aat
//
LOCUS       DATROY010000210         1250 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.211, whole genome shotgun sequence.
ACCESSION   DATROY010000210 DATROY010000000
VERSION     DATROY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.211"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..498
                     /locus_tag="VBD11_002003"
     CDS             <1..498
                     /locus_tag="VBD11_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO6954905.1"
                     /translation="NSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKLNYPSSQL
                     LQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDITTLEKGN
                     LPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGLTSLGIVP
                     DLNKL"
     gene            509..1198
                     /locus_tag="VBD11_002004"
     CDS             509..1198
                     /locus_tag="VBD11_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6954906.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
BASE COUNT          457 a          176 c          235 g          382 t
ORIGIN      
        1 aatagtatta ctgcacaaga tctacaagcg gggagttttc ttgcaaatga ctataaggag
       61 attattacgt ctactgacgt tctagaaaaa gttatttctt ctgaaaaatt gaattatcct
      121 tcgtctcagt tgctacaaaa aataacagtt tctattttaa aagatacacg tgttatttca
      181 atatcggtcg aagatgctaa tccaaaaatg tctcaaaaat tagcaaattc agttagagaa
      241 gcagcagttt caaaaatcaa ggcagttact caagtagagg atatcactac tcttgagaag
      301 ggaaatttac ctaaagcacc atcttctcct aatattaaaa agaatgtact aatcgggttt
      361 attgttggtg caggattatc aactattgtt ttagttatta tggggatttt ggatgatcgt
      421 gttaatactg aagaagatat tgaaaaggtc ttaggactta cttctttagg tatagtacca
      481 gatttgaata aactttaagg agaatataat gactcgttta gaaatagttg atagcaagtt
      541 aagacaagca aaaaaaacag aagaatactt caatgcgatc cgtacaaaca tacagtttag
      601 tggaaaggaa aataaaattc ttgcaattgc ctctgttagg gaaggggaag gaaaatccac
      661 tacttcaaca agtttagctt tatctttagc tcaagcagga tttaaaacat tattaattga
      721 tgcggatact aggaactctg ttatgtctgg aacctttaaa gcaactggaa ctattaaagg
      781 cttgacgaat tatttatcag gtaatgcaga tcttggagat attatctgtg aaaccaatgt
      841 tcctagactg atggtcgttc cttcaggaaa agtaccacca aatccaacag cattacttca
      901 gaacgcttat tttaataaga tgattgaagc tattaaaaat atatttgatt atattatcat
      961 cgatactcca cctattggtt tagttgttga tgccgcaata atcgctaatg cttgcgatgg
     1021 ttttatttta gtaacccaag caggcagaat aaaacgtaat tatgttgaaa aagcaaaaga
     1081 acagatggaa caaagtggtt caaagttctt aggtattatt cttaataaag ttaatgaatc
     1141 tgttgctact tacggcgatt atggaaatta cggaaaaagg gatagaaaaa ggaagtaagg
     1201 gactctggta ttgaaagaaa aagaaaatat acaaaagatt attatagcga
//
LOCUS       DATROY010000211         1212 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.212, whole genome shotgun sequence.
ACCESSION   DATROY010000211 DATROY010000000
VERSION     DATROY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.212"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            358..>1212
                     /gene="ltrA"
                     /locus_tag="VBD11_002005"
     CDS             358..>1212
                     /gene="ltrA"
                     /locus_tag="VBD11_002005"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6954907.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     HFSEYSYGFRPNRSCETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     QDGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLR
                     FVRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFW
                     KSPKGWKCR"
BASE COUNT          390 a          238 c          288 g          296 t
ORIGIN      
        1 gcgtatatag cggatgaggg ggctataaac tctaaagtcc aaataggtag tcgtaaccta
       61 tatacgtaaa tcacgagagt aaacgaggaa agatgtccga cttatcccgt gaggtcctgt
      121 caagggtaaa acctagtaac aacgaacgac aagaagtcag ccgaacccat agtagtgatg
      181 aagtttctgt aatggaaatg gaacgaaggg gtgaacaact aatcgaagta atcctacttc
      241 acttgtgtct gtaaaacaaa cggtctgata gaactagatg cggttacgta ttgactagag
      301 gaaggttcac caatgtaaga tatcatgcag acaacgaaaa aagaaaggaa tacgcacatg
      361 tcagaattgc tagataagat atcatctcgg aacaatatgc tcgaagctta caagcaagtg
      421 aaatcaaaca aaggttctgc tggtatcgat ggggtcacta tcgagcagat ggatgactat
      481 cttcaccaaa attggcgaga aaccaagaaa ctcatcaaag agaggagcta taaacctcaa
      541 ccggttctca gggttgaaat cccaaaacca aacggaggag ttcgtaacct aggtatcccg
      601 acggctatgg atagaatgat tcagcaggcc atcgttcaag ttttgagtcc actctgcgaa
      661 aaacattttt cagagtatag ctatgggttc agacccaatc gctcctgcga aacagccatt
      721 gttcagctac ttgagtattt aaacgatggc tacgagtgga ttgtggacat tgacttggaa
      781 aagttcttcg atactgttcc gcaagacaga ttgatgtccc tggttcataa tatcattcaa
      841 gatggcgata cggagtcact gattcgtaag tacttccatt cgggagttgt tattaacgga
      901 cagcgacata agactttagt cgggacacct caaggcggga atctatcacc cctcctatct
      961 aatattatgc ttaatgagtt agacaaaggg ttggaaaagc gaggtcttcg ctttgtccgt
     1021 tacgccgatg actgtgtcat cactgtcgga agcgaagcag ctgctaagcg ggtcatgcat
     1081 tcggtcagta gctatattga gaagcgatta gggttgaaag tcaacatgac taagaccaag
     1141 attgtcagac cgaacaaact caaatacctc ggatttggtt tctggaaatc tccaaaaggt
     1201 tggaagtgtc gt
//
LOCUS       DATROY010000212         1196 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.213, whole genome shotgun sequence.
ACCESSION   DATROY010000212 DATROY010000000
VERSION     DATROY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.213"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..>1196
                     /locus_tag="VBD11_002006"
     CDS             <1..>1196
                     /locus_tag="VBD11_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6954908.1"
                     /translation="SLEEINGTIEVPKDLSFFKTLLAYSGPGALVAVGYMDPGNWSTS
                     ITGGQNFQYLLISIILMSSLIAMLLQYMSAKLGIVTQMDLAQAIRARTSKQLGIVLWI
                     LTELAIMATDIAEVIGGAIALYLLFHIPLAIAVFITVFDVLLLLLLTKIGFRKIEALV
                     VALILVIFLVFAYQVALSHPIWTDIFKGLVPTSEAFSTSHTVNGQTPLSGALGIIGAT
                     VMPHNLYLHSSVVQSRKLDHNNKKDIARAIRFSTFDSNIQLTVAFFVNSLLLIMGVAV
                     FKTGSVTDPSFFGLFKALSNSTIMSNSILAHIASSGILSLLFAIALLASGQNSTITGT
                     LTGQIIMEGFIHMKVPIWFRRIITRLISVIPVMICVLVTSGRSTVEEHIAINNLMNNS
                     QVFLAFA"
BASE COUNT          346 a          199 c          199 g          452 t
ORIGIN      
        1 tccctagaag aaataaatgg gacaattgaa gttcctaaag atttgagctt ttttaaaact
       61 ttactagctt attctggacc gggtgcactt gttgctgttg gttatatgga tcctggaaat
      121 tggtccactt ccataactgg cggtcaaaat tttcaatatt tactaatttc aattatctta
      181 atgtctagtt taattgctat gctacttcaa tacatgtcag ctaagctagg tattgttact
      241 cagatggatc ttgctcaagc tatccgagca aggaccagta aacagttggg aattgtctta
      301 tggattctca ctgagcttgc tataatggca acagatattg ctgaagttat tggtggggct
      361 attgcacttt atttactgtt tcatattcca cttgcgattg ctgttttcat tacagtattt
      421 gatgtattac tcttattatt gttgacaaaa ataggatttc gaaaaattga agctctagta
      481 gttgctttaa tattggttat tttcttagtt tttgcatatc aagtggctct ttctcatcct
      541 atatggacgg atatttttaa aggtttagta cctacatctg aagctttctc aacctctcat
      601 acagtaaatg gacaaacacc actttcaggt gccttaggca ttattggggc aactgttatg
      661 ccacataatc tttatctaca ttcttcagtt gttcaaagtc gaaaactaga tcataataac
      721 aaaaaagata tcgctagagc aattaggttt tcaacttttg attcaaatat tcaattaaca
      781 gtagcctttt ttgttaattc actattactc attatgggcg ttgctgtttt caaaacagga
      841 agtgttactg atccatcatt ctttggtcta tttaaagcac tatctaattc tactattatg
      901 agtaattcta ttttagctca tatagcaagt tcaggtattt tatcactttt atttgcaata
      961 gctttattag catctggaca gaattcaact attactggaa cattaacagg tcaaattatt
     1021 atggaaggat ttattcatat gaaagttcca atttggttcc gtcgtatcat tactcgccta
     1081 atttctgtta ttcctgtaat gatttgtgtc ttagtaacta gtggtagaag tactgttgaa
     1141 gaacatatag ctattaataa cttaatgaat aactctcaag tcttcttagc atttgc
//
LOCUS       DATROY010000213         1166 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.214, whole genome shotgun sequence.
ACCESSION   DATROY010000213 DATROY010000000
VERSION     DATROY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.214"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            599..>1166
                     /locus_tag="VBD11_002007"
     CDS             599..>1166
                     /locus_tag="VBD11_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6954909.1"
                     /translation="MKEKVTVIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGSTD
                     NSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMIEI
                     MLKNLITENADIAEVDFDISNERDYRKKKRRNFYKVFKNNNSLKEFLSGNRVENIVCT
                     KLYKKSIIGNLRFDENLKIGEDLLFNCKL"
BASE COUNT          420 a          138 c          193 g          415 t
ORIGIN      
        1 aaaaacatat ggaaaatatt ttaatatcct tactgttgac tatatgttca tacttttctg
       61 gcgctagaat actattggtc tgtatgttgg ttttattagc ttcgcttctt ttagattata
      121 tcctttttaa aactaatttg aaattgacca agaaaaacac ttttatactt ggtatgactt
      181 tcttatttat caccgcttgt ttttcttata acatatggtc aataattgaa aaaataatta
      241 tgtacagaaa ccaaagtact atcactagga tgatagttta tcaagaaagt attattgaag
      301 ttctaaaagg aaatatttta tttggacagg gtataaggat tccatcaagt gaaggaatat
      361 tcctaggatc gcattctact tatattagtg tcttttacag gacttcttta ttaggaattg
      421 ttctttattt ttctgccttt atacttttat ataaagaagc gatttcaaaa aattataaaa
      481 tctacagatt atttttttat acgttattat gttacacgct ctttgaggaa atagatccta
      541 atcattggag tattgtatta ttattctcaa cttttggtat agtgggaagg gctaaaaaat
      601 gaaagaaaaa gtaacagtca ttatacctat atacaactca gaagcatacc ttaaagaatg
      661 tgtgcaatcc gtactacaac agactcatcc attgatagaa gttatactaa ttgatgatgg
      721 atccactgat aatagtggag aaatttgtga taatttatct caggaagata atcgcatact
      781 tgtatttcat aaaaaaaatg gaggggtctc ttcggcaagg aacctaggtc tagataaatc
      841 cacaggagaa ttcataacat ttgtggatag tgatgatttt gtagcaccga atatgattga
      901 aataatgtta aaaaatttaa tcactgagaa tgctgatata gcagaagtag attttgatat
      961 ttcgaatgag agagattata gaaagaagaa aagacgaaac ttttataaag tttttaagaa
     1021 taataactct ttgaaagaat ttttatcagg taatagagtg gaaaatattg tttgtacaaa
     1081 attatataaa aaaagtataa ttggtaactt gaggtttgat gagaacttaa aaattggtga
     1141 ggatttactt tttaattgca aactct
//
LOCUS       DATROY010000214         1143 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.215, whole genome shotgun sequence.
ACCESSION   DATROY010000214 DATROY010000000
VERSION     DATROY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.215"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..415
                     /locus_tag="VBD11_002008"
     CDS             <1..415
                     /locus_tag="VBD11_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEO6954910.1"
                     /translation="KGPYVIKQKLLQKGIKSKIIESELKAINFQDLASKISQKLYKKY
                     QNKLPLKALKDKLMQSLTTKGFDYQIGHTVIQNLEIEKDQELEDDLIYKELDKQYQKL
                     SKKHDQYELKQRIINVLMRKGYQYEDIKSALREYL"
     gene            499..1032
                     /locus_tag="VBD11_002009"
     CDS             499..1032
                     /locus_tag="VBD11_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6954911.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          475 a          169 c          180 g          319 t
ORIGIN      
        1 taaggggcca tacgtgataa aacaaaaatt attgcaaaaa ggtatcaaaa gtaaaataat
       61 tgaatctgaa cttaaagcta taaactttca agacttagca tccaaaataa gccaaaaact
      121 ctataaaaaa tatcaaaaca aacttccact aaaagcatta aaagataaat tgatgcagag
      181 cttaactaca aaaggatttg attatcagat agggcatacc gtaattcaaa accttgaaat
      241 tgaaaaagat caagaacttg aagatgacct catttataaa gaattagata aacaatacca
      301 aaaattatca aaaaaacatg accagtatga attaaagcaa cgtataatca atgtcttaat
      361 gcgtaaagga taccagtacg aagatatcaa aagtgcttta agagaatatt tataagataa
      421 taactctcaa acgatgtatt ttacagaaaa ttatgataaa ctataacaga cgtataaatt
      481 gtagaaagtt ggtaggctat gagattacct aaagaaggcg actttattac aattcaaagt
      541 tacaaacatg atggtagttt acaccgaact tggcgtgaca ccatggtatt aaaaacaacc
      601 gaaaatgccc tcattggtgt taatgatcat actttagtaa cagaaaatga tggtcgacgc
      661 tgggtgacac gagagcctgc aatagtatac tttcataaaa aatattggtt taacattatc
      721 gctatgatac gtgaaactgg tgtctcctac tattgtaatc tagcaagtcc gtatatcttg
      781 gatcctgaag cactcaagta catcgactac gaccttgatg tcaaagtatt tgcagatggt
      841 gaaaaaagac tactggatgt ggacgaatat gaacagcata aagctcagat gaactatcct
      901 accgatattg attatatatt aaaggaaaat gtaaaaatat tggtagaatg gataaatgag
      961 aataaaggcc ccttttcatc atcatatatc aatatctggt ataaacggta ccttgaattg
     1021 aaaaagcgct aactaacaca ctaaatagtg tgtttttatt aatatcaaat ttaattacaa
     1081 tactattgca aaaatatata cttaaaataa aaaaagtaga aagtgatctt tctacttttt
     1141 taa
//
LOCUS       DATROY010000215         1136 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.216, whole genome shotgun sequence.
ACCESSION   DATROY010000215 DATROY010000000
VERSION     DATROY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.216"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            112..>1136
                     /locus_tag="VBD11_002010"
     CDS             112..>1136
                     /locus_tag="VBD11_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6954912.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELR
                     SEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFY
                     TQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQY"
BASE COUNT          409 a          193 c          153 g          381 t
ORIGIN      
        1 ttttttaatt catatttggt taaaaaggta tcgactacct aattattctg ttgactttaa
       61 agaaatattc tctaaaatag ataacatatt aaatcaaagg tggtaactca tatgattgat
      121 tttctactgt tgaaatcatt acacagtctt ctaggtctaa ctattactgt ttgtgatcaa
      181 aatttttctg ttatcagaga gtataaatcc gagaaaacta tttcactttt ttacaatcat
      241 taccttattt taagtaactt tagtaaaact caacatgatt ttttatttca ttatggctct
      301 ttaggagaac tttttcttgt ccaccatatt caacaatatt atattatcat cggtccctgg
      361 cgtagcaatg ttattgaccc tttacttctt aagaaaaagc tgactgaaac acaaatcaat
      421 gctagtgaac aagactactt tattgataga ctatcccaat tacctttctt ctcacttagt
      481 caaatccgag aattactaat agtaaccaat tattgtctaa ctggggttgt gaaagataaa
      541 ttatcagagc ccttacatta ctacacgaaa gggtggagca actcctttga tctagataaa
      601 attaagcaat tttctaaaca gaatatgagt tcttacaaat atcagtatca ttttgaaaat
      661 aatattctaa aagctgttaa atcaggtagt gaatttcttt tgaaagaaac agtggaacaa
      721 tttagcaatt ctatcgtacc tataatcagt ggagatgaat tgcgatctga aaaaaactac
      781 tccatcatga tttatgatcg tctctcacag gctaccatcc aagcaggact tgatatcgaa
      841 acagcttatc gagcacgaga tcgttttata aaagaaaacg agtcaactat aagtctaaat
      901 gaagttttaa aattacgtga tactgctatc ttattctata ctcaacaagt tcattcttta
      961 aaaaaacatc tcgaaacccc tcattctcaa actattgtcg cagtgattcg atatcttgaa
     1021 aataatctaa atcgctttat taaaacagaa gaaatagcta aagaatgtca catgagtgaa
     1081 tcgaagttac gaaaactatt taagcaagaa aaacacatca ctattcaaca atattt
//
LOCUS       DATROY010000216         1115 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.217, whole genome shotgun sequence.
ACCESSION   DATROY010000216 DATROY010000000
VERSION     DATROY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.217"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(62..316)
                     /locus_tag="VBD11_002011"
     CDS             complement(62..316)
                     /locus_tag="VBD11_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6954913.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(335..586)
                     /locus_tag="VBD11_002012"
     CDS             complement(335..586)
                     /locus_tag="VBD11_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954914.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
BASE COUNT          418 a          194 c          174 g          329 t
ORIGIN      
        1 aaaaagctct tttaaactga caaaaatgtc ttagtaatga ttagtctctc aatcttatta
       61 cttagagtaa atcaagcaaa aatgctagaa aataaaaaat atatgacaaa ctattccaaa
      121 caatatgctc gccaatcgaa taatgaatgg attttgtata gtggtgcaaa ctagcgaaaa
      181 tcaaacccat accaaaatag acaacaaagt cgacgacaga tagaccatgt aagtgagacc
      241 agctaaatag tatagaagat aagatcacaa ccaagggacc actcagggag ttcccaaatg
      301 ttctcatgac aaacaacctg aacgttatct cttcttaaat tggggcaata agacagatag
      361 tcaataataa gacaactcga aagtagtgat tgacctgatt catatcagct attaatccct
      421 cctggttttg ccttattggg aaaaatgttt ttactagtag tgcccagcaa aataaaacgc
      481 taaaagcacc tattgcaaat agccattttt tgaggttaga tgatgactct tggtagttac
      541 tgattggctc agtttttgtt agcacataac tcaacagaat catcattata agattaatgc
      601 caatccatat actccaactc cactctaacc ctattgatag taaactaatc acccaatgcc
      661 caagcaattg ttattattgt gttgttgcaa ctttggcaaa gatgaatata gcaggatgga
      721 aaacagattg tttctgaaac ttcaaaggta tcttcttatc acaagctatt atttggttat
      781 ctccataatt aatctaaatg gagcaatata tcgtttttct actaactgtt gacaaataaa
      841 aaactcccct ttaaagtctg gtaacttttc gatttttacc agaaacttaa ggagagtgag
      901 gcaagaacta atagaatctt atttaaagtt ttatgattgg gagaacagaa caactatcat
      961 aattactgaa aaattatgaa aggcatggca aacaacagat ggtcaaatcc gctctgttga
     1021 aaacaaattt taaatataat tcatttcact ttctaagttt attaaacata gagtgttcag
     1081 gagaaatgat acgacctaat gataaaatca ttcct
//
LOCUS       DATROY010000217         1114 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.218, whole genome shotgun sequence.
ACCESSION   DATROY010000217 DATROY010000000
VERSION     DATROY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.218"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..285)
                     /locus_tag="VBD11_002013"
     CDS             complement(<1..285)
                     /locus_tag="VBD11_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6954915.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEY"
     gene            342..491
                     /locus_tag="VBD11_002014"
                     /pseudo
     CDS             342..491
                     /locus_tag="VBD11_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(997..>1114)
                     /locus_tag="VBD11_002015"
     CDS             complement(997..>1114)
                     /locus_tag="VBD11_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6954916.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          402 a          199 c          140 g          373 t
ORIGIN      
        1 atattcctca tattctgaaa aatcttcatc tccaaaaaac gcatttacac tttctggatc
       61 actagcctta actcgtattc cctttaattt tgtttctacc aaattttgaa attcttcatc
      121 agaaacagat gatttatttt ttatttcaaa aggaatactt tgagttttaa cagattgaat
      181 caaaaacatt tttactgctg tgctcatatc caaccctaag gattgaaaaa gttctgtagc
      241 atcatctttt aattgatcat caactcttac tgctactgtt gacataatat cacctcaaat
      301 catttttgta taactattgt atcactaaat gcaatacaac gcaaacaaaa aaggaaatcg
      361 tctttcccaa tgaagaggct ctagaatact atctagtcac actatttaat gattacaact
      421 tcaagcaaga acaacgaatc cacaaaggtt ttggccaatg tacagacaca cttgaaagcc
      481 tatttgatta acaatcccag ctttgccagt gtgtttacac aacattattg acacactcct
      541 tttattttta taaattaccc ttaaatacat ttaattactt gaaaagacaa acagtcattt
      601 tagaaaattt tgcatctaac cttacatttg attacaccta aaaattttaa atcatcaaaa
      661 gagagcctag gattaacatc ttaggctcta atgatacact tcatttacaa atttcaacta
      721 aataggatca tttcttcagt tttatagtaa gcgtccaata acaaggtata ttggatgctt
      781 acggaatagg gaagaaaatg gaagatacct cgttattgga cgcttacaca aaatcaaaat
      841 cctatcgaaa ttaataatct cgataggatt tttcaagaat taaactcgaa aaattgaaac
      901 actttaatta agtttgttga agagagatac ataactttat taatgtgttc acaccaataa
      961 atggtgaaaa gtttagtgac attttaggtc aaaagattaa tcctcttttt tcttagaaac
     1021 agataataaa ccaactgatg acataattgt caaagctaca acattaaaga atggagttgc
     1081 attctcacct gttgctggta gtttatttcc ttta
//
LOCUS       DATROY010000218         1096 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.219, whole genome shotgun sequence.
ACCESSION   DATROY010000218 DATROY010000000
VERSION     DATROY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.219"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            80..571
                     /locus_tag="VBD11_002016"
     CDS             80..571
                     /locus_tag="VBD11_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEO6954917.1"
                     /translation="MDEKLKLDRQKYPPIWLAVNMLTFGQMVNLLELMSTTNLTQISS
                     TFSCSNPELISWLKCINLARNICAHNSNIIDFKFITVSKLKEEWKDFLFEYKEGVFSN
                     RIALPFLIVLEMMGKINPKYHFRDIIDSLHKLIKDEDTAKYYGFASMNTIKEIKKKKN
                     FRG"
     gene            726..926
                     /locus_tag="VBD11_002017"
     CDS             726..926
                     /locus_tag="VBD11_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6954918.1"
                     /translation="MSQEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
BASE COUNT          397 a          167 c          190 g          342 t
ORIGIN      
        1 caaacactac ctttcctata gtgaaaataa ctttaaaaaa cagttaaaaa gagagctaag
       61 aaaagcatca tcctctgaat tggatgaaaa attaaaacta gacagacaaa aatatcctcc
      121 tatttggcta gcggtaaata tgttaacatt tgggcagatg gttaatttgc tagaattgat
      181 gtcaacaact aatttaactc aaatttcaag cactttttca tgtagtaatc ctgaattaat
      241 ttcttggtta aaatgtatta atctagcccg taatatttgc gcccacaatt caaatatcat
      301 agattttaaa tttataactg tctctaagtt aaaagaagag tggaaagatt ttttgtttga
      361 gtataaagaa ggtgtttttt caaacaggat tgctctacct ttcttaattg tcttggaaat
      421 gatgggtaaa ataaatccta aataccattt tcgagatatc attgattctt tgcataaact
      481 tattaaggac gaggatacag caaaatatta tggctttgca tcaatgaaca ctataaagga
      541 aatcaaaaaa aagaaaaact ttagaggctg acgcttggat ttctcttccc cttattgaca
      601 ccctcgcata atcatgagat aatcacacta gcaagaatcg cctgacacta gcggttcttg
      661 cttttttatt tgcctagaaa taatcaaaat gttaccatag aataaaaata ataaggagcc
      721 acattatgtc acaagaaaaa ctaaaagcaa aagttgaaca agcgtcaggc agtcttaaag
      781 aaggtgcagg gaagctaacc ggtgataaag agttagaagc aaaaggattt gtcgaaaaaa
      841 caattgctaa aggcaaagaa ctagcagatg atgctaaaga tgctgttgaa gaggcagtag
      901 atgctgtcaa agaaaaactg aaataaatat taaccgctct ctaattgagg gcggtttttt
      961 gtgtgtctag agtttgcttt caattaattg ttttaattct aataagtctt cttttgtagc
     1021 atttttgtta ataaaactac gagcagtaga tcgttttgat agataggttc tatgttctct
     1081 attgttttct gcccac
//
LOCUS       DATROY010000219         1090 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.220, whole genome shotgun sequence.
ACCESSION   DATROY010000219 DATROY010000000
VERSION     DATROY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.220"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..269
                     /locus_tag="VBD11_002018"
     CDS             <1..269
                     /locus_tag="VBD11_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6954919.1"
                     /translation="HVRYFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYI
                     KEIQYGTDIKSHWYQDGLVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            270..1040
                     /locus_tag="VBD11_002019"
     CDS             270..1040
                     /locus_tag="VBD11_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6954920.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
BASE COUNT          365 a          152 c          226 g          347 t
ORIGIN      
        1 accatgttcg ttattttgat ttagatatga atggacatgt taataatagt aagtatctag
       61 aatggatgta tgatgtgttg gacttagatt ttctaagtag ccacattcct aaaaaaattg
      121 atttaaaata tattaaggaa attcaatatg gaacggatat taagtctcat tggtatcaag
      181 atggtcttgt gacacgtcat gatattattg gtggcgatgc tattcatgct caagctagga
      241 ttgaatggca ggaaaaaaag gaggattaaa tggcttataa aggatatctt attgatttag
      301 atggtactat ttacaaaggg aaaagtcgta tcccagcagg agagcgtttt atcgaacgtt
      361 tgcaagaaaa aggcattcct tatatgttag taactaataa tacgactcgc acaccagaaa
      421 gcgttcagga aatgcttcga ggcttcaatg ttgaaacacc acttgaaaca atctataccg
      481 caactatggc tacggttgac tatatgaatg atatgaaccg tggaaaaaca gcttacgtta
      541 ttggtgaaga agggcttaaa aaagctattg cagatgcagg ttatgtggag gatacaaaaa
      601 atccagccta tgtggttgtt ggtttagatt ggaatgttac ttatgataaa ttggcaactg
      661 ctacgttagc aattcaaaat ggtgctttat ttattggaac caatccagat ttgaatattc
      721 ctactgaacg tggtttatta cccggtgctg gctccttaaa tgctttatta gaagccgcaa
      781 cgcgtataaa accagtcttt attggaaagc caaatgccat cattatgaat aaggcacttg
      841 aaattttaaa tattcctaga aatcaagcag tcatggtcgg tgataattat ttgacagata
      901 tcatggctgg tattaataat gatattgata ctttgttagt aacaacgggt tttacaacag
      961 ttgaagaagt accagattta ccaattcaac caagttatgt tttagcaagt ttagatgaat
     1021 ggacatttaa tgaaggataa gttattagta gttttaactt ggatttggat tatatcgtta
     1081 gcaactctag
//
LOCUS       DATROY010000220         1064 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.221, whole genome shotgun sequence.
ACCESSION   DATROY010000220 DATROY010000000
VERSION     DATROY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.221"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(1..294)
                     /locus_tag="VBD11_002020"
                     /pseudo
     CDS             complement(1..294)
                     /locus_tag="VBD11_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            472..672
                     /locus_tag="VBD11_002021"
     CDS             472..672
                     /locus_tag="VBD11_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6954921.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            714..902
                     /locus_tag="VBD11_002022"
     CDS             714..902
                     /locus_tag="VBD11_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6954922.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          385 a          139 c          212 g          328 t
ORIGIN      
        1 acatgctaac tttgcatcca tacgatgact caatttccaa gcaatatatt ttctagaata
       61 gagatcaatt attgcgcaga gatagacata tttcttatat ccaatagaaa tataggtgaa
      121 gtcagtagac catacttgat taggtttatc aggattaaat ttctggttga gtaagttttg
      181 aggatacgtt ttttgagtct ttttaagagc tgtttttgtc ttaacggtag ccattttagg
      241 gagcgccata ttcttcatca gacggtaaat tcttccttca gagatttttg tgtcttttaa
      301 aacaggttat aacacacaaa gtgttgtcta tttttatagt atacatccat actcgcataa
      361 ccatgagata atcactaaaa caagagtcgc ctgacactag cgactcttgt ttttatttgc
      421 taaagaaaga tatagatgtt atgattaata aaaataataa ggaggtgcag tatgtcacaa
      481 gaaaaactaa aatcaaaatt agatcaagca aaaggtggtg ctaaagaagg ctttggcaaa
      541 ataaccggtg ataaagagtt agaagcaaaa ggatttatcg aaaaaacaat tgctaaaggc
      601 aaagaactag cagatgatgc taaagatgct gttgaagggg cagtagatgc tgtcaaagaa
      661 aaactgaagt aacaaataac cgctcattta acgagcggtt atttgttatc tttttgtcta
      721 tcagaacaga tatagatgag tttaaagaag cgattgataa aggctatatt tcagggaaca
      781 cagtagcgat agtgcgtaaa aacggaaaga tatttgatta tgtgttacta cacgaagaag
      841 tgagagaaga agaggttgtt acagttgaga gagtgcttga tgtactgagg aagttatcat
      901 aataacggac caaaattcgc cccaaaaagt attttaaaat agaataaaat aaaaaaatat
      961 gctttttatt atggatgtat cgtgtatttt aacctttaat atatcctatt ttaatggtag
     1021 agaagttggc aggggacata ttaaaacctt ttttgagatg ttag
//
LOCUS       DATROY010000221         1053 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.222, whole genome shotgun sequence.
ACCESSION   DATROY010000221 DATROY010000000
VERSION     DATROY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.222"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            244..>1053
                     /locus_tag="VBD11_002023"
     CDS             244..>1053
                     /locus_tag="VBD11_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954923.1"
                     /translation="MNKKHKLLKNIEEFKTITQKRLTERGKFPYDTVHSTFEIKDENF
                     IMERLKSSGLSMGKPVDYMGVNGIPIYTKTLSIDNKFAFENNSKDSSYSSNINISEDK
                     IKENDQKILDLIVKSGANNQNLTDEEKVIAFTKYIGEITNYDNEAYRARNVDTEYYRA
                     SDLFSVTERKLAMCVGYSVTAARAFNIMGIPSYVVSGKSPQGISHAAVRAYYNRSWHI
                     IDITASTYWKNGNYKTTYSDFIKEYCIDGYDVYDPAKTNNRFKVKYMESNEA"
BASE COUNT          437 a          129 c          176 g          311 t
ORIGIN      
        1 actaatctag ttgaaaaaaa cagtgaagat aagtttattc aagagttaaa taggtataaa
       61 acagagatac caaatttcaa aggttttaat gtttggattt taggtgataa aggttattac
      121 aaaaacctta tcaatttaga agaaattaaa aatatccaag ctacattaaa aaaagaaaga
      181 aatgaagaat atgtctttgt caaattaaat gggaaaatcg cacatgatac aacagtattc
      241 ttgatgaata aaaaacataa attacttaaa aatatagagg aatttaagac gataacccaa
      301 aaaaggctaa ctgagagagg aaagttccca tatgataccg ttcattctac gtttgaaatt
      361 aaggatgaaa actttatcat ggaaagattg aaatcatcag ggttatctat gggtaaacca
      421 gttgattata tgggcgtaaa tggtatccct atttacacta aaacattatc tatagataat
      481 aagtttgcct ttgaaaataa ttcaaaagat tcttcatatt cttcaaacat taatattagt
      541 gaagataaaa taaaagaaaa tgaccaaaaa attctagatt taatagttaa atctggagct
      601 aataatcaaa acttaacaga tgaagaaaaa gtaattgctt tcactaaata tataggtgaa
      661 ataactaatt atgacaatga agcttacaga gctagaaatg tcgacacaga atattacaga
      721 gcttcagatt tattttctgt aacagaaaga aaacttgcta tgtgcgttgg ctatagtgta
      781 acagccgcta gagcatttaa tattatgggg atacctagtt atgtcgtttc tggtaaaagt
      841 cctcaaggaa tatctcatgc agctgtgaga gcgtattata atagaagttg gcatatcata
      901 gatatcacag ccagtacata ttggaaaaat ggtaattaca aaacaacgta ttccgatttt
      961 attaaagaat actgtataga tggttatgac gtttatgatc ccgcaaaaac aaataataga
     1021 tttaaagtta agtatatgga atcaaatgaa gcg
//
LOCUS       DATROY010000222         1038 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.223, whole genome shotgun sequence.
ACCESSION   DATROY010000222 DATROY010000000
VERSION     DATROY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.223"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            79..957
                     /locus_tag="VBD11_002024"
     CDS             79..957
                     /locus_tag="VBD11_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954924.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
BASE COUNT          311 a          147 c          192 g          388 t
ORIGIN      
        1 ttgagacatt taatgttgaa tattcgaact tagaggcggc gtatctcaac ttgcttagag
       61 aaaaagaaag gaaaaacaat gtttgaacta atttctagaa atcgtaaagt ttacacgcga
      121 gatcggttag cgtttttcat gtctttttta tcagttatta tcttaatttt ggtctatcag
      181 attttcttgg gaaaaattca attagatgct ataaaagcag cattgaggag taatacagta
      241 tccagtgata ctattcacat ggttaattat tggctcattg caggtttgac gactattatt
      301 tcaatgacta gtacattagg tgcctttgga gttatggtgt ccgacaaaga aaaaaaatta
      361 aatgaagatt ttaaggtcag tcctatatca aatatcaaaa ttgaattgtc ctatgctata
      421 tttgccgttt tatttggtat tgtcatgacg atgttctctt gcatctttgc tatcggtatt
      481 tttaacggct ttaattcctt gctagagtat tcagctatgg attatttaac tattttaggg
      541 atcgtttcct tagggactgt tttatctgcg gcgatgattt tacctatttt agcctttatt
      601 catactagtt cagcttttac gactcttagc acaattgtag ggacttttat tggttttatt
      661 tcaggagtat atctctcaat tggctcggta ggaaaagcat tacaacaggt tatgacatgg
      721 tttccattga ctcaaatcaa ttctcttttg aaacaggtct taatgaaggg ttctattgcg
      781 aaggtatttg acaaagccaa cgaagccact gtctctaact ataaagaatc atatggtgtt
      841 gttttgcgta atgctgatgg agaaaggctg agtaatcact ttatgttgat ttatatcatt
      901 gccctcattc ttattttatt ggcaattcat tttattatta aaaaggttaa aaaatgaaag
      961 atgataataa attaaagatt tctgaagctt ccttagaaga ttattctgaa gtggttcatt
     1021 tatttaatag gaatcatg
//
LOCUS       DATROY010000223         1036 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.224, whole genome shotgun sequence.
ACCESSION   DATROY010000223 DATROY010000000
VERSION     DATROY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.224"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            11..934
                     /locus_tag="VBD11_002025"
     CDS             11..934
                     /locus_tag="VBD11_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6954925.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          339 a          178 c          180 g          339 t
ORIGIN      
        1 aactagaatt atgagaattc aacaattaca atatgttatt aagattgtag aaactggtag
       61 catgaatgaa gccgctaaac aactctacat tacccaacct tcactttcaa acgctgttag
      121 gaaccttgag actgagatgg gtatccaaat ttttattcga aatccaaaag gaataacgtt
      181 aacaaaagat ggtatggaat ttctgtcata tgcgcgacaa attctagaac aaacagcact
      241 tcttgaagag cgttataagg gtgataatac aagtcgtgag ctgttcagcg tttcttcaca
      301 acactatgca tttgtggtta acgcttttgt agctctcttt aatggcacag acatgaccca
      361 atacgaactt tttttacgtg aaactaggac ttgggaaatt attgatgatg ttaaaaattt
      421 ccgttcagag attggtgttc tgtttcttaa tagctataat agagatgtat taaccaaatt
      481 attcgacgat aattccttaa ttgccacaac tctttttact actacacctc atattttcgt
      541 gagcaaatcc aatcccttag ctaatagaaa aaaattaagt atgaaagatt tagaagatta
      601 tccttatctg agttatgatc aaggcctgca taattctttc tacttttctg aggaaatgat
      661 gtctcaaatt cctcacccaa aatccattgt tgtcagtgat agagcaactc tgtttaattt
      721 aatgattgga ttagatggtt atacagtggc aactggtatc ctcaactcaa aattgaacgg
      781 tgacgaaatt gtcgcaatcc ctcttgacgt ggatgatgtt attgatattg tctatatccg
      841 acacgacaaa gcaaaccttt caaaaatggg acaaaaattt attgactatt tattagagga
      901 agtttcgttt aatcataaaa ataaacagtc ctgagattgt tgtactgacc cccaaaagtt
      961 ggacaattta ttttaagtga aggatttagt tctgtattgc acaggactaa gtccttttag
     1021 ttttgctttg attcgc
//
LOCUS       DATROY010000224         1019 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.225, whole genome shotgun sequence.
ACCESSION   DATROY010000224 DATROY010000000
VERSION     DATROY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.225"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..351
                     /locus_tag="VBD11_002026"
     CDS             <1..351
                     /locus_tag="VBD11_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO6954926.1"
                     /translation="TITNDKLLTASMINNYVKHNYISKPIKKKYNRRQVARLIVITAF
                     KQIFAIQEISQTLELLTADNHSEEAYNGFAACMNKEEVHDLPPVVISACQTLNLYQET
                     QKLVLELGGKNKNA"
     gene            344..1000
                     /locus_tag="VBD11_002027"
     CDS             344..1000
                     /locus_tag="VBD11_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6954927.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
BASE COUNT          314 a          199 c          151 g          355 t
ORIGIN      
        1 acaataacta acgataaact actcaccgct tcgatgatca acaattatgt caaacataat
       61 tatatatcca aacctataaa aaaaaaatac aatcgccgtc aagtagcacg attgattgtt
      121 attacagctt ttaaacaaat ttttgctatt caagaaatca gtcaaacatt agaacttctg
      181 acagctgaca atcattctga agaagcatat aacggatttg ctgcttgtat gaacaaagaa
      241 gaggttcacg atttaccacc agttgtcatt agtgcctgtc agacgttgaa cctttatcaa
      301 gaaacacaaa aattagtttt agaactagga ggaaaaaata aaaatgctta aagaaatgtc
      361 tatcaaatta agtcctcaac tttcctttgg cgaagagctt gcaaatagta tcactcacgc
      421 tgtaggagct ttgcttatgc taatcctatt accaatcacc gctgtctata gtcacaacca
      481 cttcggatta caagcagcac ttggtacctc aatattcgtc acttctttat ttttgatgtt
      541 tttatcatca agtatttacc actccatgac ttataacagt cttcaaaaat atgttctacg
      601 catgattgat cactctatga tttatattgc tattgcaggt agttatactc ctgtcgcatt
      661 gtcacttatt ggcggctggt taggatatct catcattttt ttacaatggg ggattacatt
      721 atttggtatc ctttataaaa tcttcgctcc caaaatcaat gataaattta gccttgtact
      781 ttacctcatt atgggatggc tggttatctt tattttccct gctattatca caaaaactgg
      841 accagctttt tggggattac tcttggctgg tggtatttgc tatactatcg gtgcactctt
      901 ttatgctcgc aaacgtcctt atgatcacat gatttggcac ctttttattc tattagcttc
      961 tattttacaa tacattggaa ttgtctactt catgctataa aatttaacta cattttatt
//
LOCUS       DATROY010000225         1019 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.226, whole genome shotgun sequence.
ACCESSION   DATROY010000225 DATROY010000000
VERSION     DATROY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.226"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..308
                     /locus_tag="VBD11_002028"
     CDS             <1..308
                     /locus_tag="VBD11_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO6954928.1"
                     /translation="KKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSK
                     GGNGTPQGGGEGILLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            301..645
                     /locus_tag="VBD11_002029"
     CDS             301..645
                     /locus_tag="VBD11_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954929.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT          365 a          136 c          185 g          333 t
ORIGIN      
        1 ataaaaaaat gacaaaaaaa gaacaattag cttatctcaa agagcatgaa caagaaataa
       61 ttgattttgt aaaatctcag aataaaaaga tagaatctgt acaaattgat tggaatgatg
      121 ttcgatggag taaaggggga aatggtacac ctcaaggagg aggagagggg attttacttt
      181 ttggggagat taataatgat tctgaatcaa gttggagagt tgatattgat atagaaaaag
      241 gacggctaga cctaaaaaat atgtatttag gacaacctat acgaattgga ggtaaattat
      301 ttgagtaatt ggaacttaga aagttttgat aacctatttg ctactttagc agaatcaaca
      361 tacacaaaaa gaccaagtaa ctttacatat aatgaattga atcctaagtt gctacgacaa
      421 ctgaactcag gtagttctgt tgaattggat ttctcaaaat ctgttaagaa aggaaaaaaa
      481 gttatcgaag gcggtcagaa cctccccaat gatggtattg tctacctgca accagataag
      541 tccttaaaat ctattgatga aaaagtagag gttcgcgttc ctgatgtcaa tggcggttat
      601 cactctgagc attgtgttac taattcctat cagaaaggtt actgaaagat gatgaagcag
      661 tggctctata atcactaaaa gctcatcatt gctcagtgtt taagttaact tctaaagctc
      721 gaaccttgtt aaatcaaggt ttgtttactt ttaaaagtga cattttattt ttctaaaaat
      781 aaggttatcg ggattcctca ttctaagtta ctttgtttat atgtaccttt acttaaggtt
      841 tattttcatt atttttaatt taatattttg gagtatgtac gctccattat agtattttcg
      901 aagacatctt taaaagaact gtagaagtta aaatatttta acctagtatc taacgtcata
      961 tattggtaca tagttttaaa tatccttttg ataaccttgt acatatgaaa taggctaaa
//
LOCUS       DATROY010000226          997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.227, whole genome shotgun sequence.
ACCESSION   DATROY010000226 DATROY010000000
VERSION     DATROY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.227"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            291..986
                     /locus_tag="VBD11_002030"
     CDS             291..986
                     /locus_tag="VBD11_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6954930.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
BASE COUNT          305 a          151 c          173 g          368 t
ORIGIN      
        1 ttaagaatgg tgcaagtttt ttggtagtaa aattaaaagt aactaattaa acttcaaatt
       61 aagggtaaat gacttatgag attttcttta tcctttcata aagtgagatt gcttcaatgc
      121 tgaggctatg atatactaaa actacattta tcattgctta ataatttatt tagaactgtt
      181 aggctagcta gtgctaatat taatactatt ttaaaccaat ctattagaaa aaaagtgctg
      241 agcaagattg cccagtctta tttttgtttg ccataaagtt agaggaaaat atgtctaaaa
      301 agtctcaccg tcaatatcaa atatatgaag ggctcaggtg tgcggttgca ctctgtttta
      361 tttcaggtta tatcaatgct tttacttatg taacacaagg aaaacgtttt gcaggtgtac
      421 aaacagggaa tttgttatct tttgctattc atcttagtaa taagcattat agtcaagcgc
      481 ttgctttttt gctacctata atggttttca tgttaggtca atcgtttact tatttcatga
      541 atcgttgggc gaataaacat caactgcact ggtacctatt atctagtttt gcgttgacac
      601 aggttgctat agtaacaatt atattgactc ctttcttacc atcgtcgttt actgtggcag
      661 gactagcctt ctttgcttca atacaagtag atacctttaa atcgctcaga ggagcacctt
      721 atgctaatat gatgatgact ggtaatatca agaatgctgc ttatttatta acaaagggac
      781 tttacgaaaa aaattctgat attttcttaa ttgctagaaa tacgattatt attattggag
      841 gatttatctt tggtgttgtt tgctcgactt atttttcaag taaactgggc gaatggagct
      901 tatcattaat attaattcct cttttatatg tcaacttgct gttaggccat gaattttata
      961 acctacaagt agaaaggcac tcctaatgta tggcgtt
//
LOCUS       DATROY010000227          956 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.228, whole genome shotgun sequence.
ACCESSION   DATROY010000227 DATROY010000000
VERSION     DATROY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.228"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(32..884)
                     /locus_tag="VBD11_002031"
                     /pseudo
     CDS             complement(32..884)
                     /locus_tag="VBD11_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
BASE COUNT          345 a          198 c          113 g          300 t
ORIGIN      
        1 aatcatagat aggtgaagaa gataaaacct tttatctcaa caacctaact ttataaactt
       61 ctttgcaaaa acctttcata ctattaaaaa cacgatcagc ttttttctct gtagaacaca
      121 ttgaaaaaac agttggtcca cttcctgtca ttaatgcaac atcggctcca gaatttaaca
      181 tacgttcttt tattgtactt ataactggat ttttagtaat tgtaatatcc tcgagtgaat
      241 ttcccataga tttgaccatt aactgataat ctgatgacaa aatagcagac tttaataaat
      301 caatatcaac tctgcttata gacttacaat caatatctct aaaaatggat ttagttgaaa
      361 taccaaaatc cggcttaacc agaactatcc aacatggtct caatgtcggt aagggtttaa
      421 caatttcacc tttacctaat actaacgaac atcccccacc aaggcaataa ggaacatcac
      481 taccaatttt aaaaccaata gcaaccattt cgtcatggcc catttgaaga ttccataatc
      541 gattaagagc tcttattgta gcagcagcat cagtagaccc ccccccccag tcctgcacag
      601 ataggaatgg atttttctaa tctaatatga acacctttat taataccata ttgatttttg
      661 attatatctg cagctttaaa cacatcatta tcattattta aaggcatttt gctactatca
      721 gaatcgataa caatacaatc ttcctttagc tcagaaatgg taacgtagtc attaagatca
      781 atactaacca taatcatagc taattcatga taaccatcgt cacatcgtcc tttaatatct
      841 aatcctaaat taagtttggc aggagctttc tcaaaaattt tcataaaacc tccctaataa
      901 aatatagaat atccatatta taacataaca aatgacaaat tcggacaatt aagatg
//
LOCUS       DATROY010000228          952 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.229, whole genome shotgun sequence.
ACCESSION   DATROY010000228 DATROY010000000
VERSION     DATROY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.229"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(306..389)
                     /locus_tag="VBD11_002032"
     tRNA            complement(306..389)
                     /locus_tag="VBD11_002032"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(353..355),aa:Leu,seq:caa)
     gene            complement(400..471)
                     /locus_tag="VBD11_002033"
     tRNA            complement(400..471)
                     /locus_tag="VBD11_002033"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(437..439),aa:Gln,seq:ttg)
     gene            complement(478..550)
                     /locus_tag="VBD11_002034"
     tRNA            complement(478..550)
                     /locus_tag="VBD11_002034"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:His,seq:gtg)
     gene            complement(565..635)
                     /locus_tag="VBD11_002035"
     tRNA            complement(565..635)
                     /locus_tag="VBD11_002035"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Trp,seq:cca)
     gene            complement(642..722)
                     /locus_tag="VBD11_002036"
     tRNA            complement(642..722)
                     /locus_tag="VBD11_002036"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(686..688),aa:Tyr,seq:gta)
     gene            complement(734..806)
                     /locus_tag="VBD11_002037"
     tRNA            complement(734..806)
                     /locus_tag="VBD11_002037"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(771..773),aa:Phe,seq:gaa)
     gene            complement(809..882)
                     /locus_tag="VBD11_002038"
     tRNA            complement(809..882)
                     /locus_tag="VBD11_002038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(846..848),aa:Met,seq:cat)
BASE COUNT          277 a          230 c          190 g          255 t
ORIGIN      
        1 tttaattttt tagtcgtaac atatacactg aaaaatctta ttattttata ctacctatct
       61 atcattcaca aagactttta ttacttcaga acctatgaca tttaggagtc ctgtttgaat
      121 ttcatttaaa tgttgagtct ccactaactc ctgaaaaatt tccttattat ttctgcttgt
      181 tttaaaactt caataacaat tgcaataatg aaaaacaaat ataagtaatt ttcagtaact
      241 ttttcttaaa attaccagta caaaacaaaa aaagacaagg tttagaaacc ttgtctttta
      301 aagtataccg gcggccgggg tcgaaccggc acgtccgtga ggacactgga ttttgagtcc
      361 agcgcgtctg ccaattccgc cacgccggct atcttaaaac tggggtagct ggattcgaac
      421 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc atgaaaaggc
      481 gagtgatggg aatcgaaccc acgaatgtca gagccacaat ctgatgtgtt aaccacttca
      541 ccacacccgc catattagaa aaaacacggg cagtaggaat cgaacccaca ctgaaggttt
      601 tggagacctt agttctacct ttaaactatg cccgtttact atggagagag agggattcga
      661 acccccgaac ccgaaggagc ggatttacag tccgccgcgt ttagcctctt cgctatctct
      721 cctaaggtat aaatggcgcg agacggaatc gaaccgccga cacatggagc ttcaatccat
      781 tgctctacca actgagctac cgagcctatt gcgggagcag gatttgaacc tacgaccttc
      841 gggttatgag cccgacgagc tacctagctg ctccatcccg cgatatcttt aaaggaggat
      901 gtgggattcg aacccacgca cgcttttaca cgcctgacgg ttttcaagac cg
//
LOCUS       DATROY010000229          942 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.230, whole genome shotgun sequence.
ACCESSION   DATROY010000229 DATROY010000000
VERSION     DATROY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.230"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            38..226
                     /locus_tag="VBD11_002039"
     CDS             38..226
                     /locus_tag="VBD11_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954931.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
     gene            258..551
                     /locus_tag="VBD11_002040"
     CDS             258..551
                     /locus_tag="VBD11_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954932.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            596..811
                     /locus_tag="VBD11_002041"
     CDS             596..811
                     /locus_tag="VBD11_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954933.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            814..>942
                     /locus_tag="VBD11_002042"
     CDS             814..>942
                     /locus_tag="VBD11_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 domain-containing protein"
                     /protein_id="HEO6954934.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDK"
BASE COUNT          330 a          112 c          205 g          295 t
ORIGIN      
        1 ttgtttcaaa agcataatgg ttttaggagt tactcaaatg aaaaaggaat ttattattac
       61 aggtcggatg gttgcaagag ttatggaaac cctagtttgc ttatgtatgg taagtaatct
      121 tgttcttgat atgcttgata taatagaaat aaatgaaatt gtattttgtg tgttggtagc
      181 atcaattgca gggcttgagt tttgtttgca aaaagaaagg gagtagatag atggaatttt
      241 tggcttaatg gctttttatg gtcatgacgt atattggtgt acctaaagag caaaaggcac
      301 tcagtcgtag gggtaaacaa ttagaaaaaa taaatcaggg aggaaaaaca cttatgtcac
      361 aaatgattca agatttagta ggaagcacat gtatgattta tttagaggat ggttttcaaa
      421 cgcatcctta tgagatttta aaggtagatg aggattgggt acagattcgt cgcaacttaa
      481 aacgaagtaa agaggaggta cgtattctcc gtattcatga tatcaaggaa ttgcgtccag
      541 tcaataaata gagtgaggaa taaatctata tatcatttta taaaggagaa aaatgatggg
      601 attaatttta aaaaacaggc tcaaagaatt gcgtgcacga gatggtatca atcaaacaga
      661 actcgccaaa ttaacgggag tctcaagaca aacaattagc ctaattgaac gcggagaata
      721 tacgccatct gttattattg ctatgaagat ttctcaagta tttaaagaat ccctagagaa
      781 tgtttttagt ttagtgtcaa cggaggaata acgatgaaaa attttataaa acaaagtgat
      841 actacatttg ttcgaattat caaaagtctt ttaattggtg gttttatagg ggccatactt
      901 ggttccgtag gagctctttt taacatattt ggtcaagata ag
//
LOCUS       DATROY010000230          926 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.231, whole genome shotgun sequence.
ACCESSION   DATROY010000230 DATROY010000000
VERSION     DATROY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.231"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..374
                     /locus_tag="VBD11_002043"
     CDS             <1..374
                     /locus_tag="VBD11_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6954935.1"
                     /translation="IIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQQYYSYSI
                     STLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHFLTIFSFGLVYI
                     YLLPYQTTARLIFYRNITKNS"
     gene            complement(388..>926)
                     /locus_tag="VBD11_002044"
     CDS             complement(388..>926)
                     /locus_tag="VBD11_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6954936.1"
                     /translation="RLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQL
                     VFEKENRQVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQD
                     IVIDIIDQGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILV
                     ESQYQKGSKFSLVLKLQK"
BASE COUNT          296 a          170 c          136 g          324 t
ORIGIN      
        1 taatcatcat ttcaagtttt aggctacttt tgagtggtaa ccttatcctt gcccctgttt
       61 taatagttgt tagtagccta attactacta aggcagttat aaaactcgtt caacaatatt
      121 actcctatag tatctcaact ttagtatttt acactcaatt agaaagtgga aactatgagg
      181 gaccatctaa agtactagtt gctagtcgag aactaatgaa tggtaataaa cttagactct
      241 tccttttaga cttaagtttt attggttggc atttccttac aatcttcagt ttcggattag
      301 tctatatcta tctccttcct taccaaacta ctgcgcgctt aatcttttat agaaacatta
      361 caaaaaatag ctaggaataa ttcctagcta tttttgtaat ttaagtacta aagagaactt
      421 actgcctttt tgatactgac tttcgaccaa aatatcacca ttcagttgat gtgcaagttg
      481 tcgcgcaata taaagaccta ggccatgtcc tcctgtcttc atatttcgtg aagattctac
      541 acgataaaaa cgattaaaaa tagaagcaag atcttcatct tttatcccat agccttggtc
      601 aataatatcg ataacgatat cttgacgatt tgaataggcc ttgatagtca agggacttcc
      661 aggatcagaa tatttgactg cattgctaat caaattcaat aaaattcgtg ataatttatc
      721 atattgacta gataatttcg aaacatctgg cgcaacatca atcattactt gccgattttc
      781 tttttcaaac accaattgaa attcagataa aatatcaatc agtagtttat ccagataaat
      841 tgtttcttct ttatgaggtt cagccgtgtc tgacatagtc tctaatgtaa taaaacttaa
      901 ttcttcaact agatggttaa gacggt
//
LOCUS       DATROY010000231          920 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.232, whole genome shotgun sequence.
ACCESSION   DATROY010000231 DATROY010000000
VERSION     DATROY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.232"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            58..816
                     /locus_tag="VBD11_002045"
     CDS             58..816
                     /locus_tag="VBD11_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284767.1"
                     /note="The term CAMP (Christie, Atkins, Munch-Petersen)
                     factor is used for toxins found in group A and B
                     Streptococcus. Related toxins are also found in
                     Propionibacterium acnesother and other bacterial species;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor family pore-forming toxin"
                     /protein_id="HEO6954937.1"
                     /translation="MAKKQVVYFSIAILTGVALFSPAILEVHADQATNHVVVSQVNNQ
                     QQIKQKLDQDSVQLNTIKNQVQGTDYEKPVDTAINAVDKMKSSLGGNPATIYDLGSIG
                     PRIEAITDVIEAINFSTHNLTNKVSQANVDMGFGITKLVIRIADPFASVDSIKAQVAE
                     VKQLEQKVMTYPDLKPTDKATIYTKSKLDKAIWDIRVQRDQKVLNKKSFDVYNALNKA
                     ITHAVGVQLNPNVTVQEVDQEIVNLQMALQTALK"
BASE COUNT          345 a          141 c          156 g          278 t
ORIGIN      
        1 ctacaagtta aaaatactgc agtatattaa gttattttga tttaggaggg aaaaaccatg
       61 gcaaagaaac aagtagtcta tttttccata gctattttga caggagttgc attgttttct
      121 ccagcgattt tggaagttca tgctgatcaa gctactaatc atgtcgtagt tagtcaagtt
      181 aataatcaac aacaaataaa acaaaaactt gatcaagata gtgttcaatt gaatactatt
      241 aaaaatcaag ttcaaggcac agattatgaa aaacctgtag atactgctat aaatgctgtt
      301 gataagatga agtcttcttt aggtggtaat ccagcgacaa tctacgatct tggttctatt
      361 ggtccacgta tagaagctat cacagatgtt attgaagcaa ttaatttttc aacacataac
      421 ttgactaaca aagttagtca agctaatgtt gacatgggat tcggaataac taaattagtt
      481 attcgtattg ctgatccatt tgcatctgtt gattctataa aagcacaagt agcagaagtg
      541 aagcaattag aacaaaaagt aatgacttat ccagatttga agccaacaga taaagcaact
      601 atttatacta agtcaaaact ggacaaagca atttgggata ttcgtgtaca aagagaccaa
      661 aaagttttaa acaagaaaag ttttgatgtt tataatgctt taaataaagc tatcacacat
      721 gcagtaggcg tccaattaaa tcctaatgta accgttcaag aagtggatca agagattgtg
      781 aacctacaaa tggcacttca aactgctttg aaatgagttg atactaaacc agaacgcgat
      841 tactactaat ctccggtccg ttcctatcaa attaaataat aaatatggcc tatttattaa
      901 ataaaaagaa tgatagtttt
//
LOCUS       DATROY010000232          900 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.233, whole genome shotgun sequence.
ACCESSION   DATROY010000232 DATROY010000000
VERSION     DATROY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.233"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            34..552
                     /locus_tag="VBD11_002046"
     CDS             34..552
                     /locus_tag="VBD11_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6954938.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(624..900)
                     /locus_tag="VBD11_002047"
                     /pseudo
     CDS             complement(624..900)
                     /locus_tag="VBD11_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445921.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
BASE COUNT          347 a          101 c          158 g          294 t
ORIGIN      
        1 tattaataat atgtatcaga aaggagatgt tttttgagag tttatgaaaa taaagaagag
       61 ttgaaaaaag aaataagtaa aacatttgag aaatacatta tggaatttaa taatattcca
      121 gagaatctaa aagataaaag aattgatgaa gttgatagaa ctccagcaga aaacctttct
      181 tatcaggttg gctggaccaa cttggttctt aaatgggaag aagatgaaag aaagggactt
      241 caagtaaaaa caccatcgga taaatttaaa tggaatcaac ttggtgaatt atatcagtgg
      301 ttcacagata cctacgctca tttatctctg caagagttga aagcaaaatt aaatgaaaat
      361 attaattcta tctatgcaat gattgatttg ttgagtgagg aagaattatt tgaagcgcat
      421 atgagaaagt gggctgatga agcgactaaa acagcgactt gggaagtgta taagtttatt
      481 catgtaaata cggttgcacc ttttggaact ttcagaacta aaatcagaaa atggaagaag
      541 atagtattat aaattatatt tttaacttta aaaaatttca taaaaatggt taccaaagac
      601 gatagttttt tctaatacaa atattaaatt ctcacgacag atgtaatata tcttgctatt
      661 tcttcaggct cttcagtaaa gtcatgatct atccaattta aaataatact ttcaattgtt
      721 gagatataaa taccaagagc gaaagaatat ggaacttgat atgctgtttc cagttgtttt
      781 tgtaagtctg gtatatcgga atgtgtaatc atctctttga tatagttttt aattatgttg
      841 tcaatattcg gaaaaaacaa aggatttaat aatttcttta tttatataca tatagctaag
//
LOCUS       DATROY010000233          871 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.234, whole genome shotgun sequence.
ACCESSION   DATROY010000233 DATROY010000000
VERSION     DATROY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.234"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..416)
                     /locus_tag="VBD11_002048"
     CDS             complement(<1..416)
                     /locus_tag="VBD11_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEO6954939.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIK"
BASE COUNT          310 a          120 c          134 g          307 t
ORIGIN      
        1 tttttgatat cattatatcc tgtcatacta ggtttgatac attgaataaa cttaaaagag
       61 ctagtctgca acggttgtaa ataacgaagg ctaacttggt ctacaataga atatcctatt
      121 aaatctaagt gaaatttaat gctatctgtg atatgctctt tattttcaat cggatgaata
      181 gaatgcatac catttggacg gatcaagata atatcaccag attggctatt aaaaaaatca
      241 taatcgatgt gataacgtgc ggtcccctcg tggacatatg aaatttcaag ttccggatgc
      301 cagtgaaata aaatgtcagg gtaaccattt ctaacctcag tatgcatgtg ttcatagggt
      361 aataaatgcc ggttatcttc taaattatgt gatagtaaat tatcaagtga gttcatcgtt
      421 tttctttcta aaatcgtagg atagtattat tataacagaa aactatcaaa aaatgggttt
      481 cgtaggatag tattattttt tgaaaggata gtgtaagttt tgtataggaa catgacatat
      541 aatataagtc atagagaaaa cgctttaaaa tttttgctta gagtgtttct aataacttca
      601 aaatccttac agggtcgttt aatctcctta tctcatgtaa ggattttttg ttgtattaat
      661 atgaccaaat ttattaatat actcaattat ttattttttc aaaacagaat aactatgtga
      721 aaaaaaaata atgttaggtc tctcaaaaac acgtatagca atttcgaatc tcaaatattt
      781 tattatgact attgtaatag ttacgaatct attattcaac tcaacaataa gaaaactctt
      841 cagtaaatca ttatgttaaa caagctctga t
//
LOCUS       DATROY010000234          865 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.235, whole genome shotgun sequence.
ACCESSION   DATROY010000234 DATROY010000000
VERSION     DATROY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.235"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
BASE COUNT          293 a          165 c          117 g          290 t
ORIGIN      
        1 aaaaggtatt atcatctaac acccttttca aaaataaaat atagtttagt aaaggacgaa
       61 acctcgcatt ctaaactata ttttttattt ctttttgcaa acaaaaaagc cgacttgtga
      121 aagaagtcga catagaaaaa aatttctaaa taaattacaa cacgaaaggt aaataataac
      181 ttaccttata aaaatttaaa aaatatgctc ttatcttcgc atactacttt ctcaatgttt
      241 cacacatagg tactcgttcg aataagtcca ccttattcat tagctagata gtatctaaca
      301 caccgcccac aataccatac cttatccgcc tctctacgtt tttagtaatc attgtcaaca
      361 ccccttgcga ggtagcagtt ttagtttttt gctcaacaca ggctaactaa cataaacctt
      421 agtgtgcttt tgaggtctga actttacttg tttagtcatc actagaatga taaaatatat
      481 ttggtgattc tacatcatgc ccaactcctt ttagatctac ctgaaagcaa actttgaaca
      541 gaattgactc tccagagtta gtctaataag gaggtagggt atgatagaaa ttatcaccga
      601 agtgatgttt gctatcatga aaggattctt ccaattactt ggattatact ttcattgctt
      661 agttgaacac acagtaatta ctataatcat aactgcaatt gttttgcatc aatttttata
      721 gtaaccattt gctagatagt cttctatcta gctttttttg cttaaacaag caaagttcag
      781 acctcaaaag caagccagac aacttgtgta gtatactaca cctgctttac ttttgaagaa
      841 tttcacgtta taataaactt gctag
//
LOCUS       DATROY010000235          840 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.236, whole genome shotgun sequence.
ACCESSION   DATROY010000235 DATROY010000000
VERSION     DATROY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.236"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            76..558
                     /locus_tag="VBD11_002049"
     CDS             76..558
                     /locus_tag="VBD11_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6954940.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            548..>840
                     /locus_tag="VBD11_002050"
     CDS             548..>840
                     /locus_tag="VBD11_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6954941.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFV"
BASE COUNT          286 a          113 c          178 g          263 t
ORIGIN      
        1 gtgtgctact ttgatagtaa attgcctaaa taaaggctat tttttgttat aatatgagaa
       61 tgagaggagt ttgaaatgac taaattagca acaatttgtt acattgacaa tggcaaggaa
      121 ttacttctat tgcatcgcaa caaaaaagag aatgatgttc atgagggaaa gtggatctcg
      181 gtaggcggta aactggaggc aggtgaaact ccggatgaat gtgctaagcg tgaaattcta
      241 gaagaaaccc atttaacagt aaaaaaaatg gattttaaag gagtcatcac ttttcctgaa
      301 tttacgccag gtcatgattg gtatacctat gtctttaaag taacagatta tgaaggagag
      361 ttaatttcag atgatgaatc acgagaagga accttagaat gggtaccata tgatcaagtt
      421 ctctctaagc caacgtggca aggagattac gaaattttta aatggatttt agaagatgtt
      481 cctttctttt ctgctaagtt tgtttatgat gaacaccaaa atttaattga aaagacggtt
      541 aatttttatg aaaaatagag attttagggt tagagaccag ctcgaaacat tcggagtcag
      601 agtttctgct ttaatcatag aaaatcaaaa actgctatta atatatgctc ctcatctcta
      661 taaatactat ttgccaggtg gggcgcttca agttggagaa gatagcaata aggctgttgc
      721 gagagaagta cttgaggaaa taggtcttca cagccaagtt ggtgatttag cctatattgt
      781 tgagaatcag tttaatatta agagacatca ttatcactca gttgaatttt tatattttgt
//
LOCUS       DATROY010000236          817 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.237, whole genome shotgun sequence.
ACCESSION   DATROY010000236 DATROY010000000
VERSION     DATROY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.237"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..357
                     /locus_tag="VBD11_002051"
     CDS             <1..357
                     /locus_tag="VBD11_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002284916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage resistance protein"
                     /protein_id="HEO6954942.1"
                     /translation="RDKDNNVIEITKNFQDEHEWRYIPKLNPKELPLMLVEEEDIRAE
                     EINKIYTDSISQTHDGVLKFDVSDVRYIFVDTNQNRNQLIQFIRGKRKGKRLSTQEKD
                     ILISKIMVYDELKEDW"
     gene            357..>817
                     /locus_tag="VBD11_002052"
     CDS             357..>817
                     /locus_tag="VBD11_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002266884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954943.1"
                     /translation="MVTSKFNSVFSKKTNKEINTPTALRKKLNLEAPKGYSYELMAGT
                     KDMYVLRSKKNRKENSFQVRIKFPLEFEGMILHNIDELTEAMYRTQRSFKLDESLQKD
                     PPTIIHLGSSGVTKQIIGPVETFPALDPIEVKVGREKIIVPVKRVPYSSLT"
BASE COUNT          325 a          102 c          161 g          229 t
ORIGIN      
        1 agagataaag ataataatgt catagaaata acaaagaatt ttcaagatga gcatgagtgg
       61 cggtacattc ctaaattgaa tccgaaggag cttccgttaa tgttggttga ggaagaggat
      121 attcgagctg aagaaatcaa taaaatttat acggatagca tatcacaaac gcatgatggc
      181 gtactaaaat ttgatgtaag tgatgtgagg tatatttttg ttgatactaa tcaaaataga
      241 aatcaattga ttcaatttat cagaggaaaa cgaaagggga aacgattatc tactcaggaa
      301 aaagatattc taatatcaaa aataatggtg tatgatgagt taaaggagga ttggtgatgg
      361 ttacctcgaa gtttaattcg gtattttcta aaaagactaa caaagaaata aatactccta
      421 ctgctctaag gaaaaaactc aatctggaag ctccaaaagg atattcatat gaactgatgg
      481 cgggaactaa ggatatgtat gttttacgtt caaaaaagaa tcgtaaagag aacagctttc
      541 aagtgagaat aaaatttcca ttagaatttg aaggtatgat attacataat attgatgaat
      601 taactgaagc tatgtataga actcaaagaa gttttaaact agatgaatca cttcaaaaag
      661 atcctccaac tattattcat ttaggtagtt caggggttac aaaacaaata ataggtcctg
      721 tagaaacatt tcctgctctt gatccaatag aagtgaaagt aggaagagaa aaaattatag
      781 tacctgtgaa gagagtacca tattccagct taacgga
//
LOCUS       DATROY010000237          813 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.238, whole genome shotgun sequence.
ACCESSION   DATROY010000237 DATROY010000000
VERSION     DATROY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.238"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..145
                     /locus_tag="VBD11_002053"
     CDS             <1..145
                     /locus_tag="VBD11_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6954944.1"
                     /translation="AKSVGVARQTINLIENNKYNPSLDLCIRLAECLQTDLNTLFWDS
                     RHL"
     gene            245..778
                     /locus_tag="VBD11_002054"
     CDS             245..778
                     /locus_tag="VBD11_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6954945.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
BASE COUNT          289 a          141 c          113 g          270 t
ORIGIN      
        1 agcaaaatca gttggtgtcg ccagacaaac aataaacctt attgaaaata ataaatacaa
       61 tccttcgcta gatctttgta taagactagc ggaatgccta caaacagacc tcaatactct
      121 tttttgggat agtagacatc tataacagat tagactctct aaatctatga aaactcggga
      181 ggttaggtcg tattattatt tcaatctcaa actttattaa atagttcaag gataggagaa
      241 tattatgaaa gaaaagaaaa atttggggat aaaattaata aaatccctat atggtattaa
      301 tggcccattc gatgaataca gagaacaaaa aatcaattac cttggaaatc tattatttgc
      361 aaccactagt ctctatcaca ttttatcctc tatcatcgcc tggttcacgg taagtaaata
      421 tcccacagag actgcatgta ctctcctagc tttaacattc tttctatatt tttgtatttt
      481 tctaattgtt attccctctt atctcaacag tcatgattta ataacgccac tagaagtctc
      541 aaaaaatgac tatccagtag caataaagaa aaacaaacaa aaatttatca aaatcgggat
      601 attttatact gtcatccagt ggcttatctt gggtttaaca actaacaata acactccgct
      661 gattgtatca ctattttctt taaaagttat tttgtctagt attatgtccg gtgtagctta
      721 caatggagtt atgtatctaa tatttaaaaa caatgttgat attgaaaaag atgaataatg
      781 acaacatttc atctctaatt gttctcactg tct
//
LOCUS       DATROY010000238          798 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.239, whole genome shotgun sequence.
ACCESSION   DATROY010000238 DATROY010000000
VERSION     DATROY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.239"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            4..729
                     /locus_tag="VBD11_002055"
     CDS             4..729
                     /locus_tag="VBD11_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6954946.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
BASE COUNT          274 a          108 c          160 g          256 t
ORIGIN      
        1 acaatgttaa aaattgaaaa tgttacaggg gggtatgtta atatcccagt tttaaaaaat
       61 atttcttttg aagtaaatga tggagagtta gttggattaa ttgggcttaa tggtgcagga
      121 aaatcaacca ctattaacga gattattggt attttgagac cctatcaagg agatattacc
      181 attgatggca ttagtcttga agccaaccaa gaactttata gaaagaaaat aggctttatt
      241 cctgagacac caagtctcta tgaggaacta actctcagag aacatttaga aacggtagct
      301 atggcgtatg atattgccac cgatgaagtt atggcacgtg cgcaaaaact attagagatg
      361 tttcgtctaa cagataaatt agactggttt ccaatgcatt tttcaaaggg aatgaaacaa
      421 aaggttatga ttatctgtgc ttttgttgta tctccaagtt tatttattgt tgatgaacca
      481 tttttaggtt tggatccact agctatttca gatttgatta atcttctagc agaagaaaaa
      541 gctaaaggga aatctatttt gatgtcaaca cacgtattag attctgccga aaaaatgtgt
      601 gatcgttttg ttattttaca taaaggagaa atacgggcag ttggaaccct agaggaatta
      661 cgggctattt ttggagacag taatgctaat ttaaatgaca tctacatagc tctgacaaaa
      721 gaggtgtgat atgaaaaagt tatttaataa acgtcgaagt ctctttttaa ctcaaaatag
      781 taaatatttg agatacgt
//
LOCUS       DATROY010000239          781 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.242, whole genome shotgun sequence.
ACCESSION   DATROY010000239 DATROY010000000
VERSION     DATROY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.242"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBD11_002056"
     CDS             complement(<1..684)
                     /locus_tag="VBD11_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO6954947.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPRTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          244 a          146 c           98 g          293 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtacgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acataatatt ctcctttact ttattctgta tagctctata
      721 catagtaaag tataggcttt aaaaacaaat attagaatat ctatactttt ttatccaatc
      781 t
//
LOCUS       DATROY010000240          775 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.244, whole genome shotgun sequence.
ACCESSION   DATROY010000240 DATROY010000000
VERSION     DATROY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.244"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..135)
                     /locus_tag="VBD11_002057"
     CDS             complement(<1..135)
                     /locus_tag="VBD11_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6954948.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     V"
     gene            complement(132..740)
                     /locus_tag="VBD11_002058"
     CDS             complement(132..740)
                     /locus_tag="VBD11_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6954949.1"
                     /translation="MVLNDINFAIDEGDFVSIVGPSGSGKSTVLKLASGLMSPTAGHI
                     FFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYHIRHEKVDYRRVDDLFQ
                     RFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEVTSALDNHNKAIVEKVI
                     KSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK"
BASE COUNT          260 a          153 c          105 g          257 t
ORIGIN      
        1 cactgctcgg atagcgctaa taatcacttc tttttctagg tttaactttt gccagtaaga
       61 aaaaaataaa gtaatcaaaa ctaatgacga tgccattagt aatgacatta aagaaataga
      121 atttgcaccg ctcattttat aacctccatt gcttcaatac taccattgac aacttttaag
      181 actttattgg caaatcgacg actctgttcc tcatcatgcg taatccacaa aatcgttatt
      241 cctttgtcat gtaatgattt aataaccttt tcaacaatag ctttattatg attgtctaaa
      301 gccgaagtaa cttcatctaa gagtaaaact ttaggttcaa acaataattg cctaatcaga
      361 gcaatacgtt gcttttctcc tcctgatagt tttttgacat cctgtttcag gtaagactgg
      421 tccatttcaa aacgttgaaa taaatcatca acgcgtctat aatctacttt ttcgtgtcga
      481 atgtggtaag gaaaactaat attgtcttcc actgtattac caaataaatg tggcgtttga
      541 aaacagtaag aaatcatttt tcgactttca atcggttcta attggttcaa atcttttcca
      601 tcaaagaaaa tatgacctgc agtaggactc attaaaccac tggctaattt taatacagta
      661 ctcttaccac ttccagatgg ccctacaata gaaacaaaat caccttcatc aattgcaaag
      721 ttgatatcat ttaaaaccaa cttatcatcc gttttaaaat caacgtgttt aaagg
//
LOCUS       DATROY010000241          732 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.245, whole genome shotgun sequence.
ACCESSION   DATROY010000241 DATROY010000000
VERSION     DATROY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.245"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(101..493)
                     /locus_tag="VBD11_002059"
     CDS             complement(101..493)
                     /locus_tag="VBD11_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO6954950.1"
                     /translation="MQSQKLNILQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQAFSWLIDELDFRGFDEELGWIHVFAHGADLLTEIVKMK
                     CFSKDDSSKILTIIKRVMITDDILTLNG"
BASE COUNT          231 a          136 c           85 g          280 t
ORIGIN      
        1 catgcaatca taaacttaaa ttagcatgga aataccttta cgacctccaa ggattcaatt
       61 ttaaattttt ctgaacaact gattatgatt caaaatattt tcaaccgttt aatgttagta
      121 tgtcatcagt tatcatcact ctttttatga tagttaagat ttttgaactg tcatcttttg
      181 aaaaacattt catcttgact atttctgtta ataaatcagc tccatgagca aagacatgta
      241 tccatccaag ctcttcgtca aagcctctaa aatcaagttc atctataagc caggagaatg
      301 cctgattcat aatttttatc tgatcttctt cagatatcca acttttatta tttgagtcct
      361 ccaacaataa agctagtaat agagcagtaa aagatctgtt taacacagta ttactgttgg
      421 gtactcctaa tccttgtaaa agaaatttat cccttaaaac tctctgaagg atatttaatt
      481 tctggctttg catcagttta tgttctatta ctaatttaac ccaagaccca tatactattt
      541 catctcgaac tctcgggttc caatctccta tatgatgtaa tcaaaaagat aaaacattct
      601 catcaattgg ataattattt tctatctttt tgattacatc acttgcttcc atgtttctcc
      661 ttgcatgact ttatatttta tattttccaa agtgtaacat tagagttttc gttttcactt
      721 taaaatgatt at
//
LOCUS       DATROY010000242          704 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.246, whole genome shotgun sequence.
ACCESSION   DATROY010000242 DATROY010000000
VERSION     DATROY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.246"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..704)
                     /locus_tag="VBD11_002060"
     CDS             complement(<1..704)
                     /locus_tag="VBD11_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YydG family radical SAM peptide epimerase"
                     /protein_id="HEO6954951.1"
                     /translation="MNLSQKCNAKCDHCCFSCLPNSVNCLTDEEIEAVVRYAETHEDV
                     ELVSLTGGEALLRKSKVLETIHRLSILGKDVTLITNGFWATNDKNTKSLLTSLRTAGL
                     RYLTVSYDNYHSEYIPVDNIKRLFLHIKKFDIEVALNMVVDKKNRGVDLLDKLGESIF
                     GVPITIVPASPVGRAKNLNMEDLYLKTIDELELTCPATGWEFVIHHDGYVYPCCSPSV
                     FETNLRIGSIGDADIS"
BASE COUNT          238 a          142 c           95 g          229 t
ORIGIN      
        1 tctgatatat cagcatcacc aatacttcca attctcaaat tcgtctcaaa aactgatgga
       61 gagcaacaag gataaacata accatcatga tgaatcacaa attcccagcc cgttgcagga
      121 catgtaagtt ctaattcatc tatcgttttt aaatacaaat cttccatatt tagatttttg
      181 gctctaccta ctggagatgc aggaacaatc gtaataggaa caccaaatat tgactcacct
      241 aacttatcta ataaatcaac accacgattt ttcttatcta ctaccatatt aagtgcaact
      301 tcaatatcaa acttctttat atgtaaaaat aatcttttta tattatctac tgggatatac
      361 tctgaatgat agttgtcata actaacggtt aaataacgta aaccggctgt tcttaaagaa
      421 gttagtaaac tcttcgtatt tttatcatta gttgcccaga agccatttgt aattaacgta
      481 acatctttac ctaaaatcga taagcgatga atagtttcta agactttgct ctttcttaat
      541 agtgcttctc cgccagtcaa agaaactagt tcaacatctt catgagtttc cgcatagcgt
      601 actactgctt caatttcttc atccgtaaga caattgacag agttaggtaa gcaactaaaa
      661 caacaatgat cgcactttgc attacacttt tgggagagat taat
//
LOCUS       DATROY010000243          693 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.247, whole genome shotgun sequence.
ACCESSION   DATROY010000243 DATROY010000000
VERSION     DATROY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.247"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            35..>693
                     /locus_tag="VBD11_002061"
     CDS             35..>693
                     /locus_tag="VBD11_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6954952.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
BASE COUNT          247 a           96 c          127 g          223 t
ORIGIN      
        1 tgaacgaagt tcttagacag gaaaaaaata tttaatatca cttgaagatt actatctttt
       61 atctaagaaa ttttcccaag taatgacgct cgattcgcat agttcagacg acggctatac
      121 tatccgttcg ctatactttg attctttaga agatgttgat tggcaagaaa aggaagatgg
      181 tgttgagata cgaagaaaga ttcgtttgcg caattatggt agtcaaagca attttgcgaa
      241 attagaaatg aaacaaaaac aaggtgctaa tcaaaaaaag cgctctctaa aattaagcaa
      301 gacagatgct caagcgctga ttaatggtaa caatagtgtt ttattaaatt atccggatac
      361 atttgctgcg gagtgttatt caaggataac aacgcaactt tatcaaccta aaacggttat
      421 tgagtaccaa agaaaagctt ttgttgctaa ggaaaacaat atccgaatca cctttgacca
      481 ccatataata ggtacagagg ctagttatga tattttctca gaggatttaa atcaaaatta
      541 tttattagat ccttatcttg ttattttaga agttaaatat aatggatttt tattgtctta
      601 tattaaagaa ctcttagatt cggtagatag aagtgaactt tcagcgagta agtattgttt
      661 aggaagagct gttagtaaac attatcgttt tta
//
LOCUS       DATROY010000244          689 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.248, whole genome shotgun sequence.
ACCESSION   DATROY010000244 DATROY010000000
VERSION     DATROY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.248"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..134
                     /locus_tag="VBD11_002062"
     CDS             <1..134
                     /locus_tag="VBD11_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEO6954953.1"
                     /translation="WESLQETIRLMENDYLSHKVINGISQVKEKQVTKHGLIEVEDV"
     gene            127..381
                     /locus_tag="VBD11_002063"
     CDS             127..381
                     /locus_tag="VBD11_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEO6954954.1"
                     /translation="MFNFTEEAWKDYVSWQQEDKKILKRINRLIEDIKRDPFEGIGKP
                     EPLKYHYSGAWSRRITEEHRLIYMIEDGEIYFLSFRDHYK"
     gene            434..>689
                     /locus_tag="VBD11_002064"
     CDS             434..>689
                     /locus_tag="VBD11_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6954955.1"
                     /translation="MIDIQKIKKFLLRYTKKEEGGKQPKTKEVKQRTANFIVYGILGL
                     LFIVGFFGSLRAIGLSNQVQHLKETVIAVEKKSKHKKTDDS"
BASE COUNT          275 a           70 c          137 g          207 t
ORIGIN      
        1 gctgggaaag tttacaagaa acgattcgtt tgatggaaaa tgattatcta tcacataagg
       61 ttattaatgg tatatcacaa gttaaagaga agcaagtcac taaacatggt ttaattgagg
      121 tagaagatgt ttaattttac tgaagaagca tggaaagatt atgtttcttg gcaacaggaa
      181 gataaaaaaa tcttgaaacg aattaatcgt ttgatagaag atattaaaag agacccattt
      241 gaaggcattg gaaaaccaga acctttaaaa taccattatt caggggcttg gtctcgaaga
      301 attacagaag aacataggtt gatttatatg atagaagatg gagaaatcta ttttttatct
      361 tttagagatc attataagta gaagtagtcg ctaaaaaaag tgactgcttt tttattttat
      421 agaaaggggt taaatgattg atatacaaaa aataaaaaag tttctattga gatatacaaa
      481 gaaagaagaa ggaggtaaac agcctaagac aaaagaagta aagcaaagga cagccaattt
      541 tattgtctat ggtatcttag gtttgttatt tattgtagga ttttttggat cattgagggc
      601 aattggctta tctaatcagg tacaacattt aaaagaaaca gttattgctg tagaaaaaaa
      661 gtcaaaacat aagaaaactg atgattcat
//
LOCUS       DATROY010000245          688 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.249, whole genome shotgun sequence.
ACCESSION   DATROY010000245 DATROY010000000
VERSION     DATROY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.249"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..147
                     /locus_tag="VBD11_002065"
     CDS             <1..147
                     /locus_tag="VBD11_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6954956.1"
                     /translation="EKGYISSDTVMVVRKNGKIFDYVLPHEKVREEEVVTVERVEDVM
                     RELE"
BASE COUNT          260 a           92 c          131 g          205 t
ORIGIN      
        1 gaaaaaggat atatcagcag tgatacagtg atggttgtgc gtaaaaacgg aaagatattt
       61 gattatgtgt taccacacga aaaagtgaga gaagaagagg ttgtgacagt tgagagagtg
      121 gaagatgtta tgagagaatt ggagtaatgt tcgtcgtgac ataagcagct atcaatagcc
      181 tgccccacaa aaaagcatat tatagttggg gcgaatttgg ggcataagtt aaaaactaat
      241 ctatgttaac aatgatacga cttttacgtt tactactcat aacgacttat atagatatgt
      301 tttagacctt ataatgccaa ccgaaaaaac taactcttaa agacgctgtt aaataattcg
      361 tctagaaaaa ccttgtcata tcaatgttta ttgatagcga caaggttctt ttttatatcc
      421 aatactcaat aaacgactag agagctcaaa ggaatttaga gtttcaaatg tgttagagca
      481 tacccttgaa ttatcttaac tgtgatgtta taattcctaa aagataagtt aaaagagagg
      541 ttgcgaaaga gaatgtcaga aaaagctata ctattaatta tccttattgc ttttgttatt
      601 atcgctatta taaagatagc tctgttaaag ttatatatga atacgataaa acctaaagta
      661 gataatgaag gaaacatcat aacaaagc
//
LOCUS       DATROY010000246          680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.250, whole genome shotgun sequence.
ACCESSION   DATROY010000246 DATROY010000000
VERSION     DATROY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.250"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBD11_002066"
     tRNA            complement(78..151)
                     /locus_tag="VBD11_002066"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBD11_002067"
     tRNA            complement(163..247)
                     /locus_tag="VBD11_002067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBD11_002068"
     tRNA            complement(256..327)
                     /locus_tag="VBD11_002068"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
     gene            complement(361..433)
                     /locus_tag="VBD11_002069"
     tRNA            complement(361..433)
                     /locus_tag="VBD11_002069"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:tgt)
     gene            complement(444..525)
                     /locus_tag="VBD11_002070"
     tRNA            complement(444..525)
                     /locus_tag="VBD11_002070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Leu,seq:tag)
     gene            complement(535..607)
                     /locus_tag="VBD11_002071"
     tRNA            complement(535..607)
                     /locus_tag="VBD11_002071"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Lys,seq:ttt)
     gene            complement(610..680)
                     /locus_tag="VBD11_002072"
     tRNA            complement(610..680)
                     /locus_tag="VBD11_002072"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asp,seq:gtc)
BASE COUNT          183 a          199 c          150 g          148 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaattgtaa
      361 tgccggctac atgacttgaa cacgcgaccc tctgattaca aatcagatgc tctaccaact
      421 gagctaagcc ggcaatctac taatgcgggt gaagggactt gaacccccac gccgttaagc
      481 gccagatcct aaatctggtg cgtctgccaa ttccgccaca cccgcatttc taaatgaccc
      541 gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc aactgagcta
      601 acgagtctac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg
      661 ataaccgcta cactacggga
//
LOCUS       DATROY010000247          668 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.251, whole genome shotgun sequence.
ACCESSION   DATROY010000247 DATROY010000000
VERSION     DATROY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.251"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            36..>668
                     /locus_tag="VBD11_002073"
     CDS             36..>668
                     /locus_tag="VBD11_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6954957.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVK"
BASE COUNT          194 a          111 c          106 g          257 t
ORIGIN      
        1 atctagtaaa aaaatgatag aaagataata ctaaaatgat agaattcttt tctaatatca
       61 gaacagagat tccgcagatg cctttactta tccatagttt gattttatct gtcttacctt
      121 ttctgatgtg gctgactttg gttaatagag ataagccttt gtataaaact atttggagta
      181 tccttttagg acttcagtta attacgattt atacttggtt tttctgggca aaattgcctt
      241 tatctgaaag tcttcccctt taccattgtc gaataggcat gtttgtcgtt ctcttagcga
      301 ggcctagaat attaaaggat tatttcgcat tgttaggagt agtaggtgga gtattggcaa
      361 tgattcaccc agacttttac ccttaccagt ttctacatgt gactaatatt ttcttcttta
      421 ttggacattt tgctttattt gtccttagtt tactacatct gatgacacag tccaatttgg
      481 acaaattaaa tccaaaattg attatacaac taacgcttct cattaatatg tctttgattt
      541 ttattaacct cttgactggg ggaaattatg gctttatgat gaagacacca atactaggaa
      601 taacaaatcc ctttttaaac cttttgatag tgacaactct attaagtttt ctcgtcctat
      661 ttgttaaa
//
LOCUS       DATROY010000248          664 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.252, whole genome shotgun sequence.
ACCESSION   DATROY010000248 DATROY010000000
VERSION     DATROY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.252"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(37..651)
                     /locus_tag="VBD11_002074"
     CDS             complement(37..651)
                     /locus_tag="VBD11_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6954958.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT          259 a          114 c           94 g          197 t
ORIGIN      
        1 aaaggctctt ttataattca ttggtaacta ctatttttat tgtaactctt tcaaagatac
       61 aatagcagac aaaccacaat atgacgatgc tcctagtaac aacgacccta aaatatccgt
      121 taaaaaatga ttttctaaat aaagtcttga aaaaataact gatgcaagga ttactaatgc
      181 aaatattacg aaaactactt gtgacaatac tcttctcaat ctctgtcttg caaccactaa
      241 aacaagaatc attaataaaa acgttgatat cgtatgtcca ctgggaaaac tataacctga
      301 gtcaaaaata agttgattca taggtctatg acgtgcgatg atatctttta aaagcaatgc
      361 caaagcattg gtaccaaaaa ataacataaa tagccacaca gctatccttt ggtttaaaaa
      421 atattgatac ccaaggataa caagtgctat taatgacgtt gtcaaagggc tgacaactgt
      481 tgatattatc gtcattaagt tagttaaatc ggagtttcgt atgctttgaa aaaaagctga
      541 agtgatatta tccaaataac ttatcatatc actttgagat ttaactgcta atgttaacac
      601 taaaaaagaa acaagtagta taacacactg gaaaaaggat ttcttgttca ctacataacc
      661 tcac
//
LOCUS       DATROY010000249          629 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.254, whole genome shotgun sequence.
ACCESSION   DATROY010000249 DATROY010000000
VERSION     DATROY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.254"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(27..518)
                     /locus_tag="VBD11_002075"
     CDS             complement(27..518)
                     /locus_tag="VBD11_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6954959.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKT
                     NLI"
BASE COUNT          176 a           87 c          113 g          253 t
ORIGIN      
        1 aaggggcttt tttatattaa gcaatactag attaagtttg ttttcgagtt attagtaata
       61 tctgcgatag caagccaaat ttccatttga taatcagtat cttgtggatg ttgagatgaa
      121 taaatttcta agtttggcga gttagctggt tttagattat tctcttgaaa aaatccttgt
      181 attatttttc gccatgtttc ttgaagactc gatggcattg gacctttaac agaaatgact
      241 gcataggtag cgcttgggat cctaataatt tctaaccctt ttggggcttg gacacttgtc
      301 tctacttgat aagtggcact gtaatcgaaa cgaaaatcag gttgtgtttt acaaatgcca
      361 atggcttgct ggataccttt ttcttttagt tttgatggat tatattgttt atgtaatttt
      421 tcccaaagtt taggacattc ttgatttgac tttacatttt ctaaagttac tccagcaagt
      481 atcattgctg gcttttcaac aatttgaaat gatagcataa aaatctttat tctttatgat
      541 tccttaagta attatagagt cgttaaaata gtttcttttt agaataattg tcttgttagc
      601 aatcataatt ttcttttgga atgatatgg
//
LOCUS       DATROY010000250          622 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.255, whole genome shotgun sequence.
ACCESSION   DATROY010000250 DATROY010000000
VERSION     DATROY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.255"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            141..>622
                     /locus_tag="VBD11_002076"
     CDS             141..>622
                     /locus_tag="VBD11_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954960.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVINKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     "
BASE COUNT          211 a           80 c          110 g          221 t
ORIGIN      
        1 tttgtagacg aagttaaaac cttataatat ttattttata aaattattca tcttattttt
       61 gcgatttcaa tagtgatatg gtatatttaa agtaagctac gtatagtttt accagtcaga
      121 aatagtaagg atggttgagt atgagagaaa ctgaaacaca cgatcaccaa gcgctaattc
      181 aaaaattgtt agtcagtatt cattatttaa ccttgtttcg tgatgaaatt atattagtag
      241 aaaaaacccc atctttactg gggaaacatt tttctattgc tattgtgcag aatgaactag
      301 gtgagatact ttctaaaata gaagctttga gtaagcaaaa aaaactgatt cgttctattt
      361 attggtatga tgaatcgtca tttaaagtca taaataaggc tctggctatt gtagaagaat
      421 ggattaaggg cttagataat cttttagaat tctgtcaatc acagacggtt tttcaagcaa
      481 ttttaggtga tgaacgtgca catgtatttg gaatccttat tgatgtttat acttctttaa
      541 acattataaa tacttctttg aaagaagaaa attctagacc agtgtcctta tctgatttgg
      601 ctctaggtct taaggacgaa ga
//
LOCUS       DATROY010000251          620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.256, whole genome shotgun sequence.
ACCESSION   DATROY010000251 DATROY010000000
VERSION     DATROY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.256"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            34..620
                     /locus_tag="VBD11_002077"
                     /pseudo
     CDS             34..620
                     /locus_tag="VBD11_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_088186184.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
BASE COUNT          218 a           95 c          140 g          167 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttcagga agaaaatgag cgcttgagaa cggagaatgc ctttctaaaa
      481 aagttgaggg atttgcgctt gagggacgaa gccttacaga gcgaacggca gaaacaatta
      541 gagaaatggt cacaggagga ttccgactag acctcttact tgagataact aagatagctc
      601 gtgcaactta ctattatcaa
//
LOCUS       DATROY010000252          559 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.259, whole genome shotgun sequence.
ACCESSION   DATROY010000252 DATROY010000000
VERSION     DATROY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.259"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            16..>559
                     /locus_tag="VBD11_002078"
     CDS             16..>559
                     /locus_tag="VBD11_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6954961.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDI"
BASE COUNT          192 a           83 c          107 g          177 t
ORIGIN      
        1 gggttagaat tatttatggc taatatgtat tatacagaga atcctaatgt tgaacatgat
       61 attcatgagc taaatgtcaa attattagga gaatcattca gttttttaac tgatgcagga
      121 gttttctcta aaagaatgat agattatggt agtcaagtcc ttctaaatag tttacatttt
      181 gaaaaaaata aatccttgtt agaccttggg tgtggatacg gtccgctggg aatttctcta
      241 gccaaagtgc aaggagttaa agcaactatg gttgacatta atacgagagc attggaatta
      301 gctaagaaaa atgcaacacg caatggagta gtagttgaag tctttcagtc aaacatttat
      361 gaaaatattt ctaaaacttt cgactatatt ataagcaacc cacctattcg tgctggaaag
      421 caagttgttc attctattat cgaagaaagt atctgttacc ttaatacagg cggtagcctt
      481 accattgtca ttcaaaagaa acaaggcgct ccaagtgcaa aagctaaaat gctagatact
      541 tttggtaatt gcgatattt
//
LOCUS       DATROY010000253          555 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.261, whole genome shotgun sequence.
ACCESSION   DATROY010000253 DATROY010000000
VERSION     DATROY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.261"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..>555
                     /locus_tag="VBD11_002079"
     CDS             <1..>555
                     /locus_tag="VBD11_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO6954962.1"
                     /translation="KTFYLAKQAQRNYEKLLVESREGIPLNKETFWKIDRVLSNGVKK
                     GQRIYHILKTNDLEVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIK
                     EGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIE
                     TAKHIQVIKRTLYDNKRDFFELFPV"
BASE COUNT          187 a          124 c          107 g          137 t
ORIGIN      
        1 aagaccttct atcttgctaa gcaagctcaa agaaactatg aaaaactttt agttgaatct
       61 agagaaggaa tccctttgaa caaagagacc ttctggaaaa tagatagagt gctttctaat
      121 ggggtcaaga agggccaacg tatctatcat atcctcaaaa ccaacgatct agaagtgagt
      181 tcttcaaccg tttatcgaca catcaagaaa ggctacctat ccatcacacc aatcgaccta
      241 cccagagctg tgaagttcaa aaaaaggcga aagagcacac tccctcctat tccaaaagcg
      301 attaaagaag ggcgacggta cgaggatttt atagaacaca tgaaccaatc agagctgaac
      361 tcctggcttg aaatggacac ggttattgga cggattggtg gaaaggtcct tctcaccttc
      421 aatgtcgcct tctgtaactt catctttgcc aaactgatgg attctaagac agctatcgaa
      481 actgctaaac acatacaggt cattaagagg acactctatg ataacaagag agacttcttc
      541 gaactctttc ctgtt
//
LOCUS       DATROY010000254          515 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.262, whole genome shotgun sequence.
ACCESSION   DATROY010000254 DATROY010000000
VERSION     DATROY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.262"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..>515
                     /locus_tag="VBD11_002080"
     CDS             <1..>515
                     /locus_tag="VBD11_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6954963.1"
                     /translation="FLDIEIKNDEQRGLEVAKQIRQIDPYAQIVFVTTHSELMPLTFR
                     YQVSALDYIDKGLSQEAFSRRIEDVLLYANNACSKNLLEDSFYFKSRYSQVQVPFNDI
                     LYIETSLRSHRVVLYTAKDRMEFTATLGDILKQEPRLFQCHRSFLVNPLNIFKVDRSN
                     KLIYLKNGSNC"
BASE COUNT          176 a           80 c           86 g          173 t
ORIGIN      
        1 tttcttagac attgaaataa aaaatgatga gcagaggggc ttagaggtcg caaaacaaat
       61 tagacaaatt gatccttatg ctcagattgt ttttgttaca actcattcgg aattaatgcc
      121 tttaaccttt cgttatcagg tatcagcttt agactatatc gataaaggac tttcacagga
      181 agcatttagc agaaggattg aagacgtttt actatatgct aataatgctt gcagtaaaaa
      241 cttactagaa gattcttttt attttaaatc acgctatagt caagtacaag taccttttaa
      301 cgatatcctt tatatagaaa cgtcattaag gtcacataga gttgtccttt atactgcaaa
      361 agacagaatg gaattcacag ccactttggg ggatatttta aaacaagaac ctaggctctt
      421 tcaatgtcat cgctcttttt tagtaaatcc cttgaatatt tttaaagtgg ataggtcaaa
      481 taaactaatc tatttaaaaa atggttcgaa ttgtt
//
LOCUS       DATROY010000255          451 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.268, whole genome shotgun sequence.
ACCESSION   DATROY010000255 DATROY010000000
VERSION     DATROY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.268"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..>451)
                     /locus_tag="VBD11_002081"
     CDS             complement(<1..>451)
                     /locus_tag="VBD11_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6954964.1"
                     /translation="GGNFSTDTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFY
                     SNIFGRQGYLTLTLPVTTNQIICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFV
                     VAFPEIYKIISSSNAPLFIAYFFLSYVAGTLLIYLSIAVGQLFTNKRV"
BASE COUNT          179 a           68 c           89 g          115 t
ORIGIN      
        1 acacgtttgt ttgtaaataa ttgccctaca gcgatagaga gataaatcag taatgtccct
       61 gcaacgtacg atagaaagaa ataggcgata aaaagtggag cgttcgaaga tgaaataatt
      121 ttatagattt ctggaaaagc gacaacgaac tgaccaatac ctactaaagg taatatgacc
      181 aatatgattc caataatgac aataaaaata ttaaaaatac tccaaagaag cgaagctagt
      241 agctttgaac aaataatctg attcgtagtt actgggagcg ttaaggttag atatccttga
      301 cggccgaaga tattgctata aaaacgtttg ataataatag caagggtgga taacagagat
      361 cctgaaataa ccatcgctaa caataaaacg agtattatag tgaatatttg ggcgctagta
      421 ctattagtat cagtagaaaa attgccgcca a
//
LOCUS       DATROY010000256          443 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.270, whole genome shotgun sequence.
ACCESSION   DATROY010000256 DATROY010000000
VERSION     DATROY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.270"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..>443)
                     /locus_tag="VBD11_002082"
     CDS             complement(<1..>443)
                     /locus_tag="VBD11_002082"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6954965.1"
                     /translation="GISGSVAVGKSTTSRLLQLLISRTFKDSHVELVTTDGFLYPNEK
                     LIQNGILNRKGFPESYDMESLLNFLDTIKNGITAKIPIYSHEIYDIVPNQLQTIETPD
                     FLILEGINVFQNQQNHRLYMNDYFDFSIYIDAENKQIEEWYLQRF"
BASE COUNT          142 a           62 c           78 g          161 t
ORIGIN      
        1 aaatcgttgt aaataccatt cttcaatttg tttattttca gcatcaatgt atattgaaaa
       61 atcaaaataa tcattcatgt aaagtcgatg gttttgttgg ttttgaaaga catttattcc
      121 ttctaaaatt aaaaaatctg gtgtttcaat agtttgcaat tgatttggaa cgatatcgta
      181 gatttcatga gaataaattg ggattttcgc ggttataccg ttctttatag tatctaagaa
      241 atttagaagt gactccatat cataggattc cgggaaacct ttacggttta aaattccatt
      301 ttgtatgagt ttctcgttag gatataaaaa gccatcagta gtgactaatt cgacgtgact
      361 atctttaaaa gttcgtgaaa ttagtaattg tagtaagcga ctggtagttg attttccaac
      421 agcaactgac cctgatatgc cga
//
LOCUS       DATROY010000257          425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.271, whole genome shotgun sequence.
ACCESSION   DATROY010000257 DATROY010000000
VERSION     DATROY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.271"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..380
                     /locus_tag="VBD11_002083"
     CDS             <1..380
                     /locus_tag="VBD11_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6954966.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          129 a           79 c          116 g          101 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat cttgaacgac
      361 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
      421 ggcta
//
LOCUS       DATROY010000258          417 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.272, whole genome shotgun sequence.
ACCESSION   DATROY010000258 DATROY010000000
VERSION     DATROY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.272"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            <1..>417
                     /locus_tag="VBD11_002084"
     CDS             <1..>417
                     /locus_tag="VBD11_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6954967.1"
                     /translation="DETFIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDT
                     TLEGLVEDMISTMNTKIAPYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVF
                     YNRLSAGQALQSNIAILYAMGKLGDKTSLAEDAQIN"
BASE COUNT          161 a           61 c           70 g          125 t
ORIGIN      
        1 aagatgaaac gtttattaaa aaaatggtag ctaaatatcc aaatttacta ggaagtttac
       61 ctgataaatc aaaagctatt tatcaattgg aaggatattt attcccagct acctacaact
      121 attataaaga tacaacctta gaaggacttg ttgaagatat gatttcaaca atgaatacta
      181 agatagcacc ttactataac actattaaag cgaagaatat gtctgtaaat gatgttttga
      241 cattatcatc tcttgttgaa aaagagggat caacagacga agatcgccgt aaaatagcaa
      301 gtgtctttta taaccgatta tcagccggtc aagctcttca aagtaacatt gcaattttgt
      361 atgctatggg gaaattaggt gataaaacaa gtttagctga agatgctcaa attaata
//
LOCUS       DATROY010000259          411 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.273, whole genome shotgun sequence.
ACCESSION   DATROY010000259 DATROY010000000
VERSION     DATROY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.273"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            complement(<1..>411)
                     /locus_tag="VBD11_002085"
     CDS             complement(<1..>411)
                     /locus_tag="VBD11_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6954968.1"
                     /translation="LEKVVYLKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLK
                     HFADVKYLFTLAHGLFVILTFPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAF
                     LLGFDQFFTLFHEVLFPGDSTWQFNPLTDPVIWIL"
BASE COUNT          175 a           71 c           58 g          107 t
ORIGIN      
        1 aagtatccaa attactggat ccgttagagg attaaattgc caagtagaat ctcctggaaa
       61 aagcacctca tgaaatagag tgaaaaactg atcaaaaccc aacagaaaag cacatactac
      121 aataaaaata ggcaacataa tagctatctt gaagaagcct tcatatagaa agatactttt
      181 ttgcttccat ccacgtctta aaaaataaat aactggaaaa gttagtataa caaaaagccc
      241 atgcgctaga gtaaaaagat attttacatc cgcaaaatgc tttaaaccat cctctgaaga
      301 aggaaagcta ggcatattta aatcagagat aaaaggatga gtcagatata tcatcaactt
      361 attaaaatta taataaattg tttcagcctt taaatacaca accttttcta a
//
LOCUS       DATROY010000260          401 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.denovo.276, whole genome shotgun sequence.
ACCESSION   DATROY010000260 DATROY010000000
VERSION     DATROY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.denovo.276"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            166..>401
                     /locus_tag="VBD11_002086"
     CDS             166..>401
                     /locus_tag="VBD11_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6954969.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKV"
BASE COUNT          153 a           56 c           62 g          130 t
ORIGIN      
        1 gggtgttttt ttaaaatctt ttgataacct ttccgttttg aaagtaaagt agatgtaata
       61 taaatcgctg tttgtatttt taatagatta acctattatg agttttatct gtgtaaggaa
      121 catctaaata tgctatactg cagttaaaaa aatagggagc aaatgatgtc acaaattaaa
      181 ctaacacctg aagaactccg tatttcagca caaaaatata caacaggatc acaatcaatt
      241 acagatgtgt taacagtttt gactcaagaa caagctgtta ttgatgaaaa ttgggatggt
      301 acagcatttg atagctttga agcccaattc aatgaattat ctccaaaaat cacacaattt
      361 gcacaattat tagaagatat aaatcaacaa ttattgaaag t
//
LOCUS       DATROY010000261        14462 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699702-rid18552073.guided.041, whole genome shotgun sequence.
ACCESSION   DATROY010000261 DATROY010000000
VERSION     DATROY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699702
            Sequence Read Archive: SRR7789140
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790017.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790017.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:54:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,102
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 2,000
            CDSs (with protein)               :: 2,000
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 44 of 63
            Pseudo Genes (incomplete)         :: 8 of 63
            Pseudo Genes (internal stop)      :: 21 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14462
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699702-rid18552073.guided.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="Blood and cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2013-02-25"
                     /collected_by="RMPRU"
     gene            309..623
                     /locus_tag="VBD11_002087"
     CDS             309..623
                     /locus_tag="VBD11_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6954970.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            639..1025
                     /locus_tag="VBD11_002088"
     CDS             639..1025
                     /locus_tag="VBD11_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6954971.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1054..2439
                     /locus_tag="VBD11_002089"
     CDS             1054..2439
                     /locus_tag="VBD11_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6954972.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2442..2594
                     /locus_tag="VBD11_002090"
     CDS             2442..2594
                     /locus_tag="VBD11_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6954973.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2617..3822
                     /locus_tag="VBD11_002091"
     CDS             2617..3822
                     /locus_tag="VBD11_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6954974.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            3865..4086
                     /locus_tag="VBD11_002092"
     CDS             3865..4086
                     /locus_tag="VBD11_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6954975.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4203..4700
                     /locus_tag="VBD11_002093"
     CDS             4203..4700
                     /locus_tag="VBD11_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6954976.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            4675..5181
                     /locus_tag="VBD11_002094"
     CDS             4675..5181
                     /locus_tag="VBD11_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6954977.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5165..7612
                     /locus_tag="VBD11_002095"
     CDS             5165..7612
                     /locus_tag="VBD11_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6954978.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            7615..9792
                     /locus_tag="VBD11_002096"
     CDS             7615..9792
                     /locus_tag="VBD11_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6954979.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            9789..10790
                     /locus_tag="VBD11_002097"
     CDS             9789..10790
                     /locus_tag="VBD11_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6954980.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            10787..11722
                     /locus_tag="VBD11_002098"
     CDS             10787..11722
                     /locus_tag="VBD11_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6954981.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            11967..12083
                     /locus_tag="VBD11_002099"
     CDS             11967..12083
                     /locus_tag="VBD11_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6954982.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12099..14018
                     /gene="tet(M)"
                     /locus_tag="VBD11_002100"
     CDS             12099..14018
                     /gene="tet(M)"
                     /locus_tag="VBD11_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6954983.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTSFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14137..14304
                     /locus_tag="VBD11_002101"
     CDS             14137..14304
                     /locus_tag="VBD11_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6954984.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14364..>14462)
                     /locus_tag="VBD11_002102"
     CDS             complement(14364..>14462)
                     /locus_tag="VBD11_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6954985.1"
                     /translation="QIEKYMDSFTDKELSLMESLASGINEARNIED"
BASE COUNT         4645 a         2639 c         3058 g         4120 t
ORIGIN      
        1 cataaaaact ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt
       61 tactacttat gaatgagctt tgatacgacg atttatcctt gaaaagtgaa gatataaaga
      121 tacttccaat aaaatttgaa tatttaatag gtagacactt caaaaaatga ggtgtctatt
      181 tttttacccg attttgaaag gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg
      241 tcgttcccca ctctttaaga ccatcaaaca ttcattcagc caataaaaaa gaaaggatag
      301 gtaaaaatat ggaacttaaa tttgtgattc ccaacatgga aaaaacattc ggcaatttag
      361 aatttgctgg cgaggataaa gtcgttcagc gaagaatcaa cggacggcta actgtcttat
      421 caagaagcta taatctctat tctgatgttc aaagagcaga tgatattgtg gtggtgcttc
      481 ctgctgaagc tggcgaaaaa catttcggct ttgaggaacg tgtgaagtta gtcaatccac
      541 gtattaccgc agagggctac aaaatcggca ctcgtggttt tacaaattac cttttacatg
      601 ctgacgacat gataaaagaa taaagaaaga gaggaaaaat gatgagatta gcaaatggca
      661 ttgtattaga taaagacacg acttttggag aattgaaatt ctctgctcta cgtcgtgaag
      721 tgagaatcca aaatgaagac gggtcggttt cagatgaaat caaggaacgt acctatgact
      781 taaaatccaa aggacaagga cgcatgattc aagtaagtat tcctgccagc gtgcctttga
      841 aagagtttga ttataacgca cgggtggaac ttatcaatcc cattgcggac accgttgcta
      901 ctgccaccta tcaaggagca gatgttgact ggtatatcaa ggcagacgat attgtgctga
      961 caaaggattc tagttcattc aaagctcaac cacaagcaaa gaaagaaccg acacaagaca
     1021 aatagtcgct aggtagaaag gagacttttt cgcatgaaac agcgtggtaa aaggattcgc
     1081 ccatctggta aagatttagt ctttcatttt acgatagcgt cactcctgcc tgttttcctg
     1141 ctggttgtcg gactgtttca tgtgaagaca atccagcaga tcaactggca ggattttaac
     1201 ctatcacaag cagataagat tgacattccc tatttaatta tcagtttcag tgtcgcaatt
     1261 cttatctgct tgctggtagc gtttgtattc aaacgggttc gctatgatac ggttaaacaa
     1321 ctttaccacc gtcaaaaact ggcaaagatg atacttgaaa acaagtggta tgaatctgaa
     1381 caggtcaaaa cagagggttt ctttaaagat agtgctggtc gtacaaagga aaagataacc
     1441 tacttcccta aaatgtatta tcgacttaaa aatggcttga tacagatacg ggtggaaatc
     1501 acgctgggaa aatatcaaga ccaactctta cacttggaaa agaaattaga gagtggcttg
     1561 tactgtgagc tgacggataa agagttaaag gattcctatg tggaatatac tttgctctat
     1621 gacaccatag ccagtcgtat ttctattgat gaagtagaag ctaaagatgg taaacttcgc
     1681 ttaatgaaaa acgtatggtg ggaatatgat aagctccctc atatgttgat tgctggtggt
     1741 acaggtggcg gtaaaactta ctttatactg acactgattg aagccttgct tcatacagat
     1801 tcaaaactgt atattcttga cccgaaaaat gctgaccttg cggacttagg ttctgtgatg
     1861 gcaaatgtct actatagaaa agaagacttg ctttcttgca ttgaaacatt ctatgaagaa
     1921 atgatgaaac gtagtgagga aatgaagcag atgaagaact ataagactgg caaaaattat
     1981 gcttacttag gtctcccggc acacttctta atctttgatg aatacgtcgc tttcatggaa
     2041 atgctgggaa caaaagaaaa caccgcagtt atgaataagc tgaaacagat tgtcatgtta
     2101 ggtcgtcaag ctggcttctt tctaatactg gcttgtcaac gtccagacgc aaaatattta
     2161 ggcgacggaa tccgtgatca gtttaatttc agagtggctt taggtcgtat gtctgaaatg
     2221 ggctatggca tgatgtttgg cagtgacgta caaaaggatt tcttcttaaa gcgaatcaaa
     2281 ggtcgtggct atgttgatgt aggaacaagt gtcatatcag agttttatac tccccttgta
     2341 ccaaaaggat atgatttctt ggaggaaatt aaaaagttat ccaacagcag acagtccacg
     2401 caggcgacgt gcgaagcgga agtcgcaggt gtggactgat cttgctggct ggtgtggcaa
     2461 tagccacgcc agcacttaac cccccgtatc taacaggggg gtacaaatcg acaggaaaca
     2521 gtcaaaaaaa cattagaaaa tcctttggtt acaagggatt tacaaaattt cagcgtatgt
     2581 caaatgggct ttaaaagttg acatacgcct ttttgattgg agggattttt actgaatgaa
     2641 caaacttggt tacagcattt aaaagaaaaa cgcttggctt atggactatc tcaaaaccgt
     2701 ttagctgttg cgactggtat tacaaggcag tatctaagcg atattgaaac aggaaaagtc
     2761 aagccatcag aggatttaca gcagtccctt tgggaagctc tggaacgctt caatcccgac
     2821 gctccccttg aaatgctgtt tgattatgta aggattcgct ttccgacaac agacgtacag
     2881 caggtggtcg aaaacatctt acaactgaaa ctgtcctatt ttcttcatga ggactatggt
     2941 ttctattctt attcagagca ttatgcttta ggcgacatat tcgtcctttg ctcccatgaa
     3001 ctggacaaag gagttctggt ggaattgaaa ggtcgtgggt gcagacaatt tgaaagctat
     3061 cttctggcac aacaaagaag ctggtatgag ttctttatgg acgttttggt ggctggcggt
     3121 gtgatgaaac gccttgacct tgccattaac gataagacag ggattttgaa tatccctgta
     3181 ctcactgaaa agtgccaaca ggaagaatgt atctccgtct tccgcagttt taaaagctat
     3241 cgcagtggcg aactggtacg caaagaggaa aaggaatgta tgggaaacac cctctatatc
     3301 ggttcattac aaagtgaagt ttatttctgt atctatgaaa aggactacga gcagtacaag
     3361 aaaaatgata ttcccattga agacgcagaa gtaaaaaacc gttttgagat tcgattgaaa
     3421 aatgagcgtg cctattatgc agtccgtgat ttactcgtct atgacaatcc agagcatacc
     3481 gcctttaaaa ttatcaatcg gtatatccgt tttgtagata aagacgattc caaacctcgt
     3541 tctgattgga aactgaatga agaatgggct tggtttattg ggaacaatcg tgaacgatta
     3601 aaactaacca caaaaccaga gccttactcc ttccaaagga cgctgaactg gctatctcat
     3661 caagttgccc cgaccttaaa ggttgcgatt aaacttgatg aaatcaacca gacgcaggtt
     3721 gtaaaagaca ttctcgacca tgcgaaactg acagaccgac acaagcagat tttgaagcaa
     3781 cagtcagtaa aagaacagga cgtgataaca acaaaaaaat aactcaaata caaattcatt
     3841 gaatatagag aggagaacat ttttatgaat tttggacaaa acctttataa ctggtttcta
     3901 tcaaacgctc aatcactggt gcttttagca atcgttgtga ttggcttgta tcttggcttc
     3961 aagcgtgagt ttagcaaact gattggcttt ttaattattg cgattattgc ggttggctta
     4021 gtcttcaacg ctgctggagt aaaagacatt ttactagagc tattcaatcg cattattggt
     4081 gcttaaataa aaccgttctt ttgtggaata taagtggttt tcttatgttc cgcaaaggaa
     4141 tggtacacca aacgaagtgc ggtagggatt tttgaatctc tacaaagaaa ggacgtgaat
     4201 atatggacga tatgcaagtc tatattgcga atttaggcaa atacaatgag ggcgaattgg
     4261 tcggtgcgtg gtttaccttt cccattgact ttgaggaagt caaagagaaa atcggcttga
     4321 atgatgaata tgaggaatac gccattcatg actacgagtt accctttacg gttgacgaat
     4381 acacttccat tggcgaactc aatcgactat gggaaatggt atcggaatta cccgaagaat
     4441 tacaatcgga gctatctgct ctgctcactc atttttcaag cattgaagaa ctaagcgaac
     4501 atcaagagga tattatcatt cattccgatt gtgatgatat gtatgacgtg gcacgctact
     4561 acattgaaga aacgggtgct ttaggcgaag taccagctag tcttcaaaac tatattgatt
     4621 atcaagccta tggtcgggat ttagaccttt caggaacgtt tatctcaacc aatcatggga
     4681 tttttgaaat cgtctattaa atctgtcggt acattactac tggcagattt tctattttac
     4741 ggggtggctc aatcagctac ccctattttt tatgaaagga ttgattacat gaagaaaata
     4801 cgaagctata ccagtatctg gtctgtggaa aaggtactgt attctatcaa tgattttaga
     4861 cttccgtttc ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtg
     4921 atgatacttg gcaacttgcc ccctctttcc atgatagagg gagcatttct caaatacttt
     4981 gggattcctg tggctttcac atggtttatg tctacaaaaa cttttgatgg taaaaagcct
     5041 tatggatttt tgaagtctgt cattgcttat gcactgcgac caaagctgac ctatgcagga
     5101 aaaaaagtaa cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa
     5161 ttttatggca tatccaatta aatacattga aaacaatctc gtctggaata aagacgggga
     5221 atgttatgct tactatgagc ttgttcctta caattactca tttctaagtc cagaacagaa
     5281 aatacaagtg catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaaaattca
     5341 tgctttacaa atcagtacag aatccagcat acgttctgca caagagcgtt ccaaaaatga
     5401 agtcactggc aagctcaaag cggttgccta tgacaaaatc gaccaacaga cagacgcttt
     5461 aatatccatg attggcgaaa atcaagtgaa ctaccgtttc tttatcggct ttaagttgct
     5521 tctcaacgat caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgctttgtc
     5581 tgattttgtc tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga
     5641 tgaaatcctg cgttttcaga agatggaaaa gctcttagaa aataaaatct ctcgtcgttt
     5701 caaaatccgc aggttagata aggacgactt cggctatctg attgaacacc tttacggaca
     5761 gacaggcact gcctatgaag agtatgagta ccatctatca aagaaaaagc tggataatga
     5821 aacgctgatt aaatactatg acttgattaa gcctactcgc tgtttggtgg aagaaaaaca
     5881 gcgatatttg aaaatccagc aggaagatga aaccgtctat gtagcttact ttaccattaa
     5941 cagcattgtc ggagaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca
     6001 atttacattc ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct
     6061 atctactgtc cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag
     6121 tgataatgaa accagctcca atgtggcgga agctctggaa agtgtgaatg agctggaaac
     6181 caatttagac caaagcaagg aatctatgta caagctgtct tatgtggtaa gggtatcagc
     6241 aaatgatctt gacgaactca aacgtcgttg taatgaagtg aaagattttt atgacgattt
     6301 aagcgtaaaa ctggtacgac catttgggga tatgctcggc ttacatgaag aatttttacc
     6361 tgccagcaag cgttatatga atgattatat tcaatacgtg acctctgatt tcctcgctgg
     6421 tttaggtttt ggtgctactc aaatgctggg ggaaaatgag gggatttatg ttggctacag
     6481 cttagatact ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg
     6541 ttcagtaacc aatgcgttag cgtcggcttt tgttggttcg ctgggtggtg gtaaatcctt
     6601 tgcgaataac cttatcgtct attatgcggt gctttatggg gcacaagcag tgattgtaga
     6661 cccaaaagca gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa
     6721 tatcgtcact ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtgattat
     6781 gaaaaatccc aaagattctg aatcactggc tattgatatt ctgacattcc ttacggggat
     6841 ttcctctcgt gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa
     6901 tagtgaagta cgagggttga tgaaagtgat tgaggaatta cgggttgaga atacgccact
     6961 aagtaccagt atagccgacc atatcgaaag ttttacagac tatgactttg cacatttatt
     7021 attcagtaat ggttatgtgg agcagtctat cagcttagaa aaacaactga acattataca
     7081 ggttgcggac ttggtacttc ccgacaagga aacttccttt gaggaatata ccactatgga
     7141 gcttttatcc gttgctatgc tgattgtcat tagtaccttt gctttagact ttatccatac
     7201 agaccgaagc attttcaaga ttgtagattt agacgaagca tggagctttt tacaggtagc
     7261 acaaggaaaa acactatcta tgaagctggt tcgggctggt cgtgctatga acgctggggt
     7321 atatttcgtg acccaaaata cagacgacct cttagatgaa aaactgaaaa ataacctcgg
     7381 cttaaaattt gcatttcgtt ccactgacct taacgagatt aaaaagacct tagccttttt
     7441 tggtgtagac ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca
     7501 atgccttatc agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga
     7561 agaactgctc catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa
     7621 ccatcaatag taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca
     7681 atgacagtgg ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg
     7741 gttcaagctg cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac
     7801 ccacttgaaa actatcaact ggatttttat gtggataata gctggggctg gcttccgtgg
     7861 aactggtcgg acgggattgg aaaacaggtc atgtatggac tatatgccat taccaatttt
     7921 atttggacaa tcagtttgta tgtttccaat gcgacaggtt acttagtaca ggaagcctat
     7981 tccttagact tcatttccgc tacagcagat tccattggta agaatatgca gaccttagct
     8041 ggtgtgagtg caaacggatt ttcaacagag ggtttctatg ttggattcct cttactcttg
     8101 attttggttc ttggggttta tgttgcctat acgggactga taaagagaga aaccacaaag
     8161 gcaattcatg ccattatgaa ttttgtgctg gtgtttatcc tatcggcttc ctttattgcc
     8221 tacgctcccg actacattaa aaaaatcaat gacttttcat cagacatcag taatgccagt
     8281 ttatcacttg gcacgaagat tgtcatgccc cattccgata gtcaaggcaa ggacagcgtg
     8341 gacttaatca gagatagcct gttttccata caggttcagc aaccgtggct actgcttcaa
     8401 tacaacagtt cagacattga aagtatcggt attgaccgtg tggaaagcct gctctccacc
     8461 agcccagatt ccaacaatgg cgaagacaga gaaaaaattg ttgcggaaga aattgaagac
     8521 agaagcaata ccaatctaac cattacaaag accattaacc gtttaggtac agtcttcttc
     8581 ctatttgtct tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt
     8641 ttctcgcagg tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc
     8701 atgattccat catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt
     8761 ttgacacgag ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc
     8821 tataccttat cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt
     8881 gcaggaatct acttcaagct gggcgattta atgagtatgt tttctctaca gagtaacgat
     8941 tctcaaagtg tgggaagtcg tgtgatgaga aaacctcgta tgcttatgca cgctcacatg
     9001 caccgtctac agcggaaact tggacgttcc atgactactc taggggctgg gtctgccatt
     9061 gttacaggta aaaaaggaca gtcgggttcg gggagttctg caaggacaca agcagatcac
     9121 tcccgaccag acggaaagga aaaatcaaca cttggaaaac gtatcggtca aaccatcggt
     9181 acagtagctg ataccaaaga cagaatggta gacactgcta gtggtttgaa agaacaggtt
     9241 aaagatttgc cgaccaatgc aagatatgca gtatatcaag gaaaatccaa agtaaaagag
     9301 aatgtccgtg atttaaccag tagtatttct caaaccaaag cggacagagc cagtggacgc
     9361 aaggaacagc aggaacaaag gcgaaaaacc attgcgaagc gtcgctctga aatggaacag
     9421 gtcaaacaga aaaaacagcc tgcttcttct gttcatgaaa gaccgactac aagacaagaa
     9481 caatatcatg atgaacagac ctcaaaacag tctaatattc agacttcata taaggaatct
     9541 caacaagcca aacaagagcg tccagcagtt aagtccgatt tttcaagtcc aaaagtggaa
     9601 cgccaaggca ataccgttca agaaaaaacc gttcaaaagc cagcaacttc aaccactaca
     9661 gcagatagaa cttcacaacg tccaatcaca aaagaacgtc cgtctactgt tcaaagagta
     9721 ccactacaaa atacaagaag tagaccacca atcaaaaccg ccaccattaa gaaagtcggt
     9781 aagaaaccat gaagttgaaa actttagtga ttggtggttc tggattattc ttgatggtct
     9841 tctcactgct tctgtttgtt gccattttat tttcagatga acaggacagc ggaatttcca
     9901 atattcatta tggaggtgtg aatgtttccg cagaagtgct ggctcataag cctatggtag
     9961 aaaaatatgc caaagaatat ggcgttgaag aatatgtcaa catacttctt gcgattatac
    10021 aggtggaatc gggcggtact gcggaagatg ttatgcagtc ctcggaatcc ctcggtcttc
    10081 cacctaattc attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgaat
    10141 tattagccag tagcgaaagg ctcagtgtag atttagaatc ggttatccag tcctacaatt
    10201 atggtggtgg tttcttaggg tatgtggcta atcgtggaaa taaatatacc tttgaactgg
    10261 ctcaaagttt ctcaaaagag tattcaggtg gcgaaaaagt gtcttacccc aatcccatag
    10321 ccatacctat caatgggggc tggcgataca actatggcaa tatgttttat gtgcaactgg
    10381 taacgcagta tcttgtcaca acagagtttg atgatgatac ggtacaagcc atcatggacg
    10441 aagcactgaa atatgagggc tggcgatacg tttacggtgg agcttccccg actacttctt
    10501 ttgattgtag cggactgaca caatggacgt atggaaaagc tggaattaac ttaccacgaa
    10561 ccgcacaaca gcaatatgat gtgacccagc atatcccact atcggaagca caagctggcg
    10621 atttggtttt ctttcattct acctataacg ctggctctta tattactcat gttgggatat
    10681 accttggcaa taaccgtatg tttcatgcag gcgacccaat cggttatgcc gacttaacaa
    10741 gcccctactg gcaacagcat ttagtgggag caggacgaat caaacaatga gaaaggaaga
    10801 tttaatgatg aaatttagaa aaaatcagaa taaagaaaaa cagataccaa aggaaaagaa
    10861 acctcgtgtc tactataagg tcaatcctca taaaaaggtt gtgattgcct tgtgggtact
    10921 tttagggctt agtttcagct ttgcgatatt caagcacttt acagctatag atactcatac
    10981 tattcacgaa acaactatca tagaaaagga atacgttgat actcatcatg tagaaaattt
    11041 tgtagagaac tttgcgaaag tctactattc atgggagcaa tccgataagt ccattgataa
    11101 tcgaatggaa agtctaaaag gctatctgac agatgaactt caagctctca atgttgatac
    11161 agtacgcaaa gatattcctg tatcgtcttc tgtaagagga tttcagatat ggacggtaga
    11221 gccaactggc gacaatgagt ttaatgtaac ctacagtgta gaccagctca ttacagaggg
    11281 agaaaataca aagaccgtcc actctgctta tatagtgagt gtctatgtag atggttctgg
    11341 aaatatggta ctggttaaga atccgaccat taccaacata cctaagaaat caagttataa
    11401 accaaaagcc attgaaagtg aggggacggt tgattccatt acaaccaatg aaatcaatga
    11461 gtttttaacg acgttcttca agctctatcc tacagcgaca gccagtgaac tttcctacta
    11521 tgtgaatgac gggatattaa aaccaatcgg aaaagagtac atctttcaag aactggtaaa
    11581 tcctattcac aatcgtaagg ataatcaagt cacggtatcg ctgacagtgg agtatatcga
    11641 ccagcagacc aaagcaacgc aggtatctca atttgatttg gtacttgaaa agaacgggag
    11701 taattggaag attatagaat aacaaatatt ggtacattat tacagctatt ttgtaatcac
    11761 gtactctctt tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat
    11821 ttaagtatct atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt
    11881 caatttgata gtgggaacaa ataattggat gtcctttttt aggagggctt agttttttgt
    11941 acccagttta agaatacctt tatcatgtga ttctaaagta tccggagaat atctgtatgc
    12001 tttgtatgcc tatggttatg cataaaaatc ccagtgataa gagtatttat cactgggatt
    12061 tttatgccct tttgggcttt tgaatggagg aaaatcacat gaaaattatt aatattggag
    12121 ttttagctca tgttgatgcg ggaaaaacta ccttaacaga aagcttatta tataacagtg
    12181 gagcgattac agaattagga agcgtggaca gaggtacaac gaaaacggat aatacgcttt
    12241 tagaacgtca gagaggaatt acaattcaga cggcgataac ctcttttcag tggaaaaata
    12301 ctaaggtgaa catcatagac acgccaggac atatggattt tttagcagaa gtatatcgtt
    12361 cattatcagt attagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
    12421 aaactcgtat attgtttcat gcacttagga aaataggtat tcccacaatc ttttttatca
    12481 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
    12541 tttctgcgga aattgtaatc aaacagaagg tagaactgca tcctaatatg cgtgtaatga
    12601 actttaccga atctgaacaa tgggatatgg taatagaagg aaatgattac cttttggaga
    12661 aatatacgtc tgggaaatta ttggaagcat tagaactcga acaagaggaa agcataagat
    12721 ttcataattg ttccctgttc cctgtttatc acggaagtgc aaaaaacaat atagggattg
    12781 ataaccttat agaagtgatt acgaataaat tttattcatc aacacatcga ggtcagtctg
    12841 aactttgcgg aaaagttttc aaaattgagt attcggaaaa aagacagcgt cttgcatata
    12901 tacgtcttta tagtggcgta ctgcatttgc gagattcggt tagaatatcg gaaaaggaaa
    12961 aaataaaaat tacagaaatg tatacttcaa taaatggtga attatgtaaa atcgataagg
    13021 cttattccgg ggaaattgtt attttgcaga atgagttttt gaagttaaat agtgttcttg
    13081 gagatacaaa gctattgcca cagagagaga gaattgaaaa tcccctccct ctgctgcaaa
    13141 cgactgttga accgagcaaa cctcaacaaa gggaaatgtt acttgatgca cttttagaaa
    13201 tctccgacag tgacccgctt ctgcgatatt atgtggattc tgcgacacat gaaatcatac
    13261 tttctttctt agggaaagta caaatggaag tgacttgtgc tctgctgcaa gaaaagtatc
    13321 atgtggagat agaaataaaa gagcctacag tcatttatat ggaaagaccg ttaaaaaaag
    13381 cagagtatac cattcacatc gaagttccac cgaatccttt ctgggcttcc attggtctat
    13441 ctgtagcaca gcttccatta gggagcggag tacagtatga gagctcggtt tctcttggat
    13501 acttaaatca atcgtttcaa aatgcagtta tggaggggat acgctatggc tgtgaacaag
    13561 gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata
    13621 gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct
    13681 taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac
    13741 aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca
    13801 ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag
    13861 aatatcgtag tgatttaact tcctttacaa atggacgtag tgtttgttta acagagttaa
    13921 aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga
    13981 tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa
    14041 atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta
    14101 aaggaggaga tagttattat aaactacaag tggatattgt gtcctgtatg tggaaataaa
    14161 acacgattaa agataaggga agatactgaa ttaaaaaaat tccccctcta ttgtccgaaa
    14221 tgcagacaag aaaatttaat tgaaataaag cagttcaaag taactgtgat tacagagcca
    14281 gacgcaaaga cgcagagccg ataaaatgag attaatacaa tctcatttta tcggctcttt
    14341 ccgttatgta tggattcttt taattagtct tcgatgtttc ttgcttcgtt gataccgctg
    14401 gctaaagatt ccattaagga tagttctttg tctgtaaagc tatccatgta tttctctatc
    14461 tg
//
